LOCUS       DASYCU010000001         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_569, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000001 DASYCU010000000
VERSION     DASYCU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGM24_00005"
     CDS             complement(<1..383)
                     /locus_tag="VGM24_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4153765.1"
                     /translation="MRPIQMVDLRQQYVRIRQQVDAAIREVLDTAAFINGKAVTDFAG
                     SLSAYLDIPHVIPCANGTDALQIALMGLGLQPGDEVITPSFTFIATTEVVALLGLKPV
                     FADVDPKTFCMDPVSLEKQISPKTKA"
     gene            503..1771
                     /locus_tag="VGM24_00010"
     CDS             503..1771
                     /locus_tag="VGM24_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEY4153766.1"
                     /translation="MSILLYNLFLLLYQMAIRLISPWNKKAAAWVRGRRGVVAQIRQS
                     GVGNRGQVVWMHCASLGEFEQGRPVMEAIRKSYPGATLLITFFSPSGYSIRKDYPGAD
                     YVFYLPADSGPHARIFLDLVKPVLVLWIKYDYWFYYLRELHRRRIPTLLISPLFRPDQ
                     PFFKWYGNLHRLMLDSFQAFFVQNEASKKLLGSLGTRQAVIVSGDTRFDRVIDIAAGF
                     QPIPEIAAFCGSSRVVVAGSTWEEDEEELDHYANTHPEIRFIIAPHEIEEDDLRDTEA
                     LFHNSIRYSAWLKRQAAGSSGPEPHVLIIDNIGMLSSLYAYATVCYVGGGFGDDGVHN
                     VLEAAVYGKPVLTGPVIEKYAEVVDLAEAGGVIIIDNALEVESVLDRLFKNSGEYAFH
                     CRAALDFVYARQGATKKILAYIQENRLLTS"
     gene            complement(1744..2412)
                     /locus_tag="VGM24_00015"
     CDS             complement(1744..2412)
                     /locus_tag="VGM24_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153767.1"
                     /translation="MERLEHLISKLNEQSEQKDSPLQMLVTLKQIEAELTRMHTVNAT
                     GRKNPGVSVVMPSLNITHRVLANVAEEKAPETKQESIESAPRPAPAPQPWMGDPLVEV
                     PTLSHQPAGPELNDFIGSGSPSINDRMKENGSADRGSQLKGSPVKELRKAIGVNDRFV
                     FINELFRGDEPMYERSIKTINNFRILPEAEYWMERELKIKLGWDDSREIVQHFYQLVS
                     RRFS"
     gene            2499..>3051
                     /locus_tag="VGM24_00020"
     CDS             2499..>3051
                     /locus_tag="VGM24_00020"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY4153768.1"
                     /translation="MFIEPAIASGRRGWVEAICGSMFSGKTEELIRRLRRAEIARLKV
                     AVFKPAADTRYHPQNIVSHNESSLLSVPVAAATVISELAVEAEVVGIDEAQFFDDELP
                     AVCDAMASRGTRVIVAGLDMDFLGRPFGPMPVLLAKADYITKLHAICVQCGNIGHYSY
                     RKFGNPGLVELGKDDLYEPRCRSC"
BASE COUNT          644 a          756 c          869 g          782 t
ORIGIN      
        1 gcttttgttt tcgggctgat ctgtttttcc agggaaacgg gatccataca aaaggtcttc
       61 gggtcaacgt cggcaaatac cggcttaagt cccagaagag caactacttc cgttgtggca
      121 ataaaggtaa aggagggcgt gatcacctcg tcgccgggct gcaaaccgag ccccatcagg
      181 gcgatctgga gcgcgtccgt gccgttggca cagggaataa catggggtat gtccaggtag
      241 gccgaaaggc ttccggcaaa gtcggttacc gctttcccgt tgataaatgc cgccgtatcc
      301 aggacctccc ggatggctgc gtccacctgc tgcctgatcc tgacatactg ctgccggaga
      361 tccaccattt gaataggccg catactgttg tcattaacgg gcacaaacct acgctaaaat
      421 aatggttcag gcaatggttc aatcagttat gaacggtttc gggaaagggt tacatttgcg
      481 ggataaccta cgtccatttc atttgagcat cctgctgtac aatcttttcc tgctcctgta
      541 ccagatggcc atccggctga tttcgccatg gaataaaaag gcggcggcat gggttcgcgg
      601 gagaagggga gtcgtcgctc agatccgcca gtcgggcgtc ggaaaccggg ggcaggtggt
      661 ctggatgcat tgcgcctccc tgggtgagtt tgaacaggga agaccggtga tggaagccat
      721 ccggaaatcc tatccgggag ctacgctgct gatcactttt ttttcccctt cgggatacag
      781 catccggaag gattatcctg gagcggatta tgttttctac ctgccggctg attccggccc
      841 tcatgcgcgg atttttcttg atctcgttaa gcccgtgctg gtgctttgga taaaatatga
      901 ctattggttc tattatctgc gcgaattgca ccggcggaga attcccaccc tgctgatttc
      961 tccgcttttc cggcccgacc agccattttt taaatggtat gggaatctgc accggctcat
     1021 gctggatagc ttccaggctt ttttcgtcca gaatgaagcc tctaaaaaat tgctgggaag
     1081 tctgggtacc cggcaggccg ttatcgtcag tggggacacc cgtttcgacc gcgttatcga
     1141 tatcgcggca ggttttcagc cgataccgga aattgccgct ttttgtggca gcagccgggt
     1201 ggttgtggcc ggcagcacct gggaagaaga cgaggaggag ctggaccatt atgccaatac
     1261 gcatcctgaa atacggttta tcatcgcgcc ccacgaaatc gaagaggatg atttacggga
     1321 cactgaagcc cttttccaca acagcatacg gtattcagcc tggttaaaac gccaggccgc
     1381 cgggtcttcg gggccggagc cccacgtgct gatcatcgat aatatcggga tgttgtccag
     1441 tttatatgct tacgccacgg tatgttatgt gggcggcgga ttcggcgatg acggtgtgca
     1501 caatgtgctg gaggccgccg tatacggcaa gcctgtgcta acgggaccgg taatcgaaaa
     1561 atatgcggaa gtggtggatc tcgcggaagc gggtggcgtg atcatcatcg acaatgcgct
     1621 tgaagtggaa tcggtgctgg accggctgtt caaaaattcc ggggaatatg cgtttcactg
     1681 ccgggcggcg ctggattttg tttatgcgcg gcagggagcc accaaaaaaa ttctggctta
     1741 tattcaggag aaccgcctgc tgacgagctg ataaaaatgt tggacgattt cgcggctatc
     1801 gtcccatccg agttttattt tcaattccct ttccatccag tattcggctt ccggaaggat
     1861 cctgaaattg ttgatggttt tgatgctccg ctcatacatg ggttcatcgc cgcggaacaa
     1921 ttcattgata aacacgaagc ggtcgttcac gccaatggcc tttctcaatt cctttaccgg
     1981 ggatcctttt aattgcgaac cgcggtctgc cgatccgttt tctttcatcc ggtcgttgat
     2041 cgaaggactt cccgaaccga tgaagtcatt taattcaggc cccgcgggct gatgagagag
     2101 ggtcggcact tccacgaggg gatcgcccat ccagggctgt ggcgcgggtg ccggccgggg
     2161 agcggattca atgctttcct gttttgtttc gggagccttt tcttccgcaa cgttagcgag
     2221 cacgcggtgc gtgatgttca gcgaaggcat gaccacggaa acaccgggat tttttcttcc
     2281 ggtggcgttg accgtatgca ttcgtgtgag ttccgcttca atctgtttga gggtcaccag
     2341 catttgcagg ggactgtcct tttgttcgga ttgttcgttt aacttgctga tcagatgttc
     2401 tagtctttcc atcgtgtggc ttcaggcaaa gcgcatttgg taaatgagcg gcggtataaa
     2461 attaaagcaa atatttaacc aaactgattt atgtcaagat gtttattgaa cctgccatag
     2521 cctcgggtcg ccggggatgg gtcgaagcga tctgtggttc gatgttctcc gggaaaacgg
     2581 aggaactgat ccggagactg cgccgggcgg agatcgcgcg gctgaaagtg gctgttttca
     2641 aaccggctgc cgatacccgg tatcatccgc aaaatattgt ttcccataac gaatccagtt
     2701 tgttgtcggt tcccgtagcc gctgcgaccg taatttccga actggccgtg gaggccgaag
     2761 tagtcggcat cgatgaggcc cagttttttg atgacgaact acccgccgtt tgtgacgcca
     2821 tggcgtcccg cggaacccgc gtcattgttg cggggctgga catggatttt ctgggaaggc
     2881 ctttcggacc aatgccggtg ctgctggcta aggcggatta cataaccaag ttgcatgcca
     2941 tttgtgtgca atgcggaaat atcgggcact attcctaccg gaaattcgga aatcccggtc
     3001 tggtggaact cgggaaggac gatctgtacg agccccgctg ccgttcctgt t
//
LOCUS       DASYCU010000002         5298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3624569, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000002 DASYCU010000000
VERSION     DASYCU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3624569"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1244
                     /locus_tag="VGM24_00025"
     CDS             282..1244
                     /locus_tag="VGM24_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY4153769.1"
                     /translation="MQIAQSILREELIVFEKKFREAVKSQAPLLDRIMRFIVNRKGKQ
                     LRPMFVFLSARLCGPVNESTYRAASLVELLHTATLVHDDVVDESLERRGFFSTYALWK
                     NKVSVLVGDYLLAKGLLLSLDHDDFRILQLLSGAVRKMSEGELLQIEKARSLNLDESV
                     YFEIIRNKTASLLASSCAAGAYSSSGNEQLTEKLSRFGEAVGIAFQIKDDLFDYGNEQ
                     TGKPSGNDLKEKKLTLPLIYTLNTVDRSLRRELIYIIKNQNRDPAKIKYVVGRVVEAG
                     GIRYSTEKMNRYRDEALAILYEFEDSEVRRGLEELVRFTTDRKY"
     gene            1274..2986
                     /gene="recJ"
                     /locus_tag="VGM24_00030"
     CDS             1274..2986
                     /gene="recJ"
                     /locus_tag="VGM24_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY4153770.1"
                     /translation="MVKRWNIRNADEEKTIALQKVLKISPVLCRILTARGLESFEEAR
                     NFFRPDIRQLHDPWLMKDMDRAVDRLFQVIDQKERILIFGDYDVDGTTAVACMYLMLS
                     RIYDPSFVNYYIPHRHREGYGISKAGIDYAKENGYRLIISVDCGIKSIELVDYAAKAG
                     IDFIICDHHLPDSELPAAVAILNPKQTGCRYPYKDLCGCGVAFKLITALCGKLGRPEA
                     DYFCFLDLVAIAIAADIVPMTGENRTLAFYGLKKLNEEPNHGIRALMQLSGLKQPVSI
                     SSLVFMIAPRVNAAGRMDDARKAVQMFVAGDFDESLAFAKLLHADNSDRKEADLNITE
                     EALALIEGDELMRNRKSTVLYRSHWHKGVVGIVASRLIEKYYRPTVVLTLSGELISGS
                     ARSVLGFNLYEAIHACREYLLGYGGHFAAAGMTMAPEKVDLFSEKFEEIVSATIDPQL
                     LVPELVIDAEIQFREINFPFFHILDQMEPFGPENRKPLFISRQVCDTGHSRLLREKHI
                     RFSLRQEDQVISGIGFNMADKIGLLEKPVDIVFTLDENDWNGKKSIQLKVVDLKCAET
                     PQPA"
     gene            complement(2983..4836)
                     /gene="htpG"
                     /locus_tag="VGM24_00035"
     CDS             complement(2983..4836)
                     /gene="htpG"
                     /locus_tag="VGM24_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY4153771.1"
                     /translation="MMQKGSIRVQTENIFPIIKKFLYSDHEIFLRELISNAVDASQKL
                     KTLSSLGEAKGELGDLTIRVKLDKEAQTIRISDQGIGMTAEEVDKYLNQVALSGAEEF
                     VNKYKNQNENAIIGKFGLGFYSAFMVSSRVEVISKSFKEEAPAVKWECDGSPEFSLEE
                     AARTGRGTDIILHINEDSKEFLDEDRIRSILEKFCRFLPIPVSYEDKQINNTRPAWTR
                     KPNELTTQDYQDFYKELYPFSEAPLFWIHLNVDYPFNLTGILYFPKIKQSYEIQKDKI
                     QLYSNQVFVTDEVKDIVPEFLMLLQGVIDSPDIPLNVSRSYLQGDPNVKKINSHITKK
                     VADKLEEIFTSERKEYEQKWESLGLFVKYGMMTDDKFLEKANRFHIMEDADGSRFYTL
                     DEYRIATETLQKNKEGKQVIIYTSNPVQQDVFIREAKAKGFKVVRLETIVDAGFINQM
                     EAKWDSVHFARVDSDVVDNLVDKQDATETVLGKEEEEKLKALFKMDMPALHITVEVRG
                     LSPEALPVLATRPEFMRRMKDMAALNGGMGAFYASMPDEVTLTINGNHSIYKTILAEG
                     DGDKQQKLVHNLADLALLSQGLLKGNELTDFIQRSVELIGGGEKNRIILEA"
     gene            4996..>5298
                     /gene="rpsF"
                     /locus_tag="VGM24_00040"
     CDS             4996..>5298
                     /gene="rpsF"
                     /locus_tag="VGM24_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY4153772.1"
                     /translation="MNNYELMVIFTPVLSEEDFKAAQTKYTAFVKDNGGKVMHENPWG
                     LKSLAYPIQKKTTGLYWVLEYSAPSDFNEKLKIQILRDETVLRFMATRLDKYAVEYN"
BASE COUNT         1319 a         1283 c         1291 g         1405 t
ORIGIN      
        1 attgtcctga gcgaaagatt gtgaaagtaa agccaaaaag aaaatcacta acaacgagta
       61 cattttcccg gtcataaact gaagtttgat atgaacaaag tgattaaaac caattataca
      121 ctttagcaaa aatataagtt aacccttaaa tggcaaattt tttatcgttt aaagaaactg
      181 aaaaccacag atttgtattg aattagctct aatttcatgc cggccgattt gggtatctgc
      241 ttctccacca tataaaatag taatttgccg cccgtaattt tatgcagata gcccaatcca
      301 tacttcgcga agagctgatt gtttttgaga agaaattccg ggaagcagta aaaagtcagg
      361 ctcccctgct tgaccggatc atgcggttta ttgtgaacag aaagggcaaa cagctgcgac
      421 ccatgtttgt atttttgtct gcccggctct gcggcccggt aaatgaatcc acctaccggg
      481 cggcttccct ggttgaactg ctgcacacgg ccaccctcgt tcatgatgat gtggtggacg
      541 aatcactgga aagaagaggg tttttttcca cctatgcctt atggaagaac aaggtgtctg
      601 tcctcgtggg agactacctg ctggccaagg ggttactact ttccctggac cacgacgatt
      661 tcagaatttt acaactgtta tccggagcgg tccgtaaaat gagtgagggg gaattgcttc
      721 aaattgaaaa agcgcgctcg ctcaacctgg atgaatccgt ctattttgaa atcatccgga
      781 acaaaacggc ttctctgctg gcttcctcct gtgctgccgg cgcctattcc agcagcggaa
      841 atgaacagct gacggaaaag ctcagccgtt tcggggaagc cgtgggcatt gcttttcaga
      901 taaaggacga cctgtttgat tacggcaatg agcaaaccgg caaaccatcg ggaaatgatc
      961 tgaaagaaaa aaagttaacc ctgccgctga tctataccct gaatacggtg gaccggtccc
     1021 tgcgacggga actgatctac atcattaaaa accagaaccg ggatcccgcg aaaattaaat
     1081 atgtcgtggg ccgcgtggtg gaagccggag ggatccgtta ttccacggaa aaaatgaacc
     1141 ggtaccggga tgaggccctg gccattctct atgaatttga agacagtgaa gtgaggaggg
     1201 gtctggaaga gctggtgcgg ttcacgacgg acaggaaata ttagctggcg ggtgttggtt
     1261 gaataaattt ttcatggtaa aaaggtggaa tatcaggaat gcggatgaag aaaaaacaat
     1321 cgctcttcaa aaggtgctaa aaatcagtcc ggttctctgc cggatactca cggcacgcgg
     1381 gttggaaagt tttgaagaag cgagaaattt ttttcgtccg gatatccgtc agcttcacga
     1441 tccctggctg atgaaggata tggatcgggc ggtggaccgc ctgttccagg tcatcgatca
     1501 gaaagaaagg atattgattt tcggagatta tgatgtggat ggcaccaccg cggtggcctg
     1561 catgtacctg atgctttccc ggatttacga tccttctttc gtaaattatt atatccccca
     1621 ccgccatcgc gaaggctacg gaatttccaa agcgggtatt gactacgcaa aagagaacgg
     1681 gtacaggctg atcatttccg tagactgcgg aataaaatcg atcgaactgg tggattacgc
     1741 agcaaaggca ggaattgatt ttatcatttg cgatcaccat cttccggatt cggaactgcc
     1801 ggcggcagtg gccattctca atcccaagca aaccggctgc cgttacccgt acaaggatct
     1861 ctgcggatgc ggggtggctt tcaaactgat tacggccctg tgcggcaaac tgggccggcc
     1921 ggaagcggat tatttttgtt tcctggacct ggtcgccatc gccattgccg cggacattgt
     1981 tccgatgacc ggcgaaaacc ggacacttgc cttttacggt ttaaaaaaac tgaatgagga
     2041 gccgaatcac ggaatccgcg ccctgatgca actcagcggg ttaaaacagc ccgtttccat
     2101 cagttcgctg gtgttcatga ttgccccgag ggtaaatgcg gccggaagga tggacgacgc
     2161 ccgcaaggcc gtacaaatgt ttgttgcggg tgatttcgat gaatccctcg cctttgcaaa
     2221 actgctgcac gcggataatt cggaccggaa agaagcggac ttgaatatca cggaggaagc
     2281 acttgcactt attgaaggag atgagctgat gcgaaaccgg aaatccactg ttttataccg
     2341 gtcgcactgg cataaggggg tggtcggcat cgtcgcgtcg cggctgatag aaaaatatta
     2401 ccgcccaacg gtggtcctta ccctgagcgg ggaacttatt tcaggaagtg cccgcagcgt
     2461 actgggcttt aatttatacg aagccattca cgcctgccgc gaatacctgc tgggttatgg
     2521 cggccatttt gctgcggccg gaatgacgat ggctcccgaa aaggtggatc tcttcagtga
     2581 aaaatttgaa gagatcgttt cagcgaccat cgacccgcag ctgttggtgc cggaactggt
     2641 cattgacgcg gaaattcaat tccgggagat caatttcccc ttttttcata tcctggatca
     2701 gatggaacct ttcggtccgg agaacaggaa accacttttc atcagccggc aggtgtgtga
     2761 taccgggcat tcccgtctgc tcagggaaaa acatatccgg ttttccctgc gccaggagga
     2821 ccaggtgata tccggaatcg gtttcaatat ggccgacaaa atcggattgc tggaaaaacc
     2881 cgtggatatt gtttttacac tggatgagaa tgactggaac gggaagaaaa gcattcagct
     2941 caaagtggtt gacctgaagt gcgctgaaac gccacagccc gcttaagcct ccagaataat
     3001 ccggttcttc tcccctcccc cgatcaactc cacgcttcgc tgaatgaaat cagtcagttc
     3061 attccccttg agcaatccct gggacaataa agccagatcg gccaggttat gaaccagttt
     3121 ctgttgttta tccccgtcgc cttcagccaa aatggtctta taaatggagt gattcccgtt
     3181 tatggtgagc gttacttcgt cgggcatgct cgcataaaaa gcacccatgc ctccgttaag
     3241 agctgccata tctttcattc gccgcatgaa ttcaggcctt gttgccagca ccggcagggc
     3301 ttccgggctc agtccccgga cctccaccgt tatgtgcaat gcgggcatat ccattttaaa
     3361 caaggctttc agtttttcct cttcctcctt tcccagaacg gtttcggtgg catcctgctt
     3421 gtcgaccagg ttgtccacca catcgctgtc cacccgggcg aaatgaacgg aatcccattt
     3481 tgcttccatc tggttgataa aaccggcgtc aacaatagtt tccagccgga ccactttaaa
     3541 tccctttgcc ttggcttccc ggataaaaac gtcctgctga accgggttgc tggtataaat
     3601 gatcacctgt ttgccttcct tgtttttctg cagcgtttcc gtggcaatcc gatattcatc
     3661 cagggtatag aaccgggaac catcggcgtc ttccatgata tggaaccggt tggccttctc
     3721 caggaacttg tcgtcggtca tcataccata tttcacgaac aggcccaggc tttcccattt
     3781 ttgttcatat tcttttcttt cactggtaaa gatctcttcc agtttgtctg ccactttttt
     3841 ggtgatgtgg ctgttgatct tttttacgtt cgggtctccc tgcaaataac ttctgctcac
     3901 attcagggga atatccgggc tgtcgatcac cccctgcaaa agcatcagga attccggaac
     3961 aatatctttg acttcgtcgg taacaaacac ctggttactg taaagctgga ttttatcttt
     4021 ctggatctca tagctctgct tgatctttgg aaaatatagg atgcccgtga ggttaaaggg
     4081 ataatccaca ttcaggtgta tccagaaaag cggggcttcg ctgaaaggat ataattcctt
     4141 ataaaaatcc tggtaatcct gggtggtcag ttcatttggt ttgcgggtcc aggccggacg
     4201 ggtattattg atctgtttgt cttcataact tacgggtatg ggcaggaagc ggcagaattt
     4261 ttccaggatg gaacggatgc ggtcctcgtc caggaattcc ttgctatcct cattgatatg
     4321 aaggatgata tcggttcctc ttccggttct ggcggcttct tccaggctga attccgggct
     4381 gccgtcgcac tcccatttta ccgcgggagc ctcttcctta aaacttttgc tgatcacttc
     4441 cacccggctg ctgaccatga aagcagagta aaatcccagc ccgaatttcc cgattatggc
     4501 attttcgttt tggttcttat atttatttac gaactcttcg gcgccggaaa gcgccacctg
     4561 gttgagatac ttgtccactt cttcggccgt catgccgatt ccctggtcgg aaatacggat
     4621 ggtttgggcc tctttatcga gtttaacccg tatggtcagg tccccgagct cccccttggc
     4681 ttccccgagg gaagaaaggg tctttagttt ttgggaagca tctaccgcgt tgctaatcag
     4741 ctctctgagg aagatttcat ggtcgctgta aagaaatttc ttaatgatgg ggaagatgtt
     4801 ctctgtctga acccggatgc ttcctttttg cataataagt agattttagc cttgccgctc
     4861 aaacagaatg ccattgcttg cttctctgac atgttgacgg aaaataccca tcatccatcc
     4921 ggtccggcta ttcgtttttt cccccaaaac ccctaccttc gcagcccaat taattttttt
     4981 accaaaaaca gggaaatgaa caattatgaa ttgatggtga tttttacccc tgtgctgtct
     5041 gaggaggact ttaaagccgc ccagaccaaa tacacggcct tcgtcaaaga taacggcggc
     5101 aaagtgatgc acgaaaatcc ctggggtctt aaatcactgg cgtatcccat ccagaagaaa
     5161 acgaccggtt tatactgggt tctggagtac agtgcgccat ccgacttcaa tgagaagctg
     5221 aaaattcaga ttctccgcga tgaaaccgtg ttacgtttca tggctaccag actcgataaa
     5281 tacgccgtcg agtacaat
//
LOCUS       DASYCU010000003         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2030273, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000003 DASYCU010000000
VERSION     DASYCU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030273"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VGM24_00045"
     CDS             <1..785
                     /locus_tag="VGM24_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY4153773.1"
                     /translation="MFIWLLLRIIDFVALLLKKKAHLPGGQKDHQMLVFFRDFLKVIL
                     VIIGILMVFSMALGFEVSKLWTGLGIAGAALALSTKESIENLIASFIIFFDKPFQTGD
                     ILKVNNITGTVEKIGLRSTRIRTDQKTYVTVPNKQMVDSIVDNQTLRTQRKGEVRLQL
                     DSSTNSDTIQRLIEECKRILSKTYIDSPSVFLNDISGTAFQLNIDYFTPPIDAGDFNE
                     IKQKVNLEILKLLETLKVPLSGSSVSVKLMGEENKKEKQADA"
     gene            complement(847..1470)
                     /locus_tag="VGM24_00050"
     CDS             complement(847..1470)
                     /locus_tag="VGM24_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY4153774.1"
                     /translation="MDTFSQISLNFIEEPVLRQDPPPGQDDKPMNIGIRIKKLKYPEN
                     KEPEEVSVEAPETGHSESQVSGSNPDSPFQKPRSGRGRKSIRQISEEADLIQIPDDEQ
                     LFSKQYYTMGEVSDMFRVNQSLLRFWEAEFSILQPKKNKKGDRYFRPVDIKNLHLIYH
                     LLRQRKYTIEGAKDFLRKSKKAEEKFESIKKLEQVKGFLLEMKAMIR"
     gene            complement(1528..>2042)
                     /locus_tag="VGM24_00055"
     CDS             complement(1528..>2042)
                     /locus_tag="VGM24_00055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4153775.1"
                     /translation="DINGFIKNKEQLQACTPAIQPVSNKDLKRIASGFGYRVDPVYKT
                     TKFHAGLDFAAPLGTPIYATANGTVETAGNTGNGYGNNVVIHNGYGYSTLFGHMFRIK
                     VHSGEKVKRGEIIGWVGSTGKSTGPHCHYEVHRNGEPVDPVYYFYNDITPEQYDRLLK
                     LASASNQSFD"
BASE COUNT          518 a          491 c          468 g          565 t
ORIGIN      
        1 tcatgtttat ctggctgctg ctgcgcatca ttgatttcgt ggctttgctg ctgaaaaaga
       61 aagcccatct tccgggcgga caaaaagacc atcagatgct ggttttcttc cgggactttc
      121 taaaagtgat cctggtcatc attggtatcc tgatggtttt cagcatggca ttagggtttg
      181 aagtgagcaa gctctggacc ggtttgggta ttgccggcgc tgcactggcc ctgtccacca
      241 aagaaagcat cgaaaacctc attgcatcct ttattatctt ttttgataag ccatttcaaa
      301 caggcgacat tctgaaagtg aacaacatca cgggaaccgt ggaaaagatc ggcctccgga
      361 gtacaaggat caggacagac cagaaaacct atgttactgt ccccaataaa cagatggtgg
      421 acagcatcgt tgacaatcaa acccttcgca cccagcgaaa aggagaggtc cgcctgcagc
      481 tggattcatc aacgaattca gatacgattc agcgtttaat agaagaatgt aagaggatac
      541 tcagtaaaac ctatatcgat tccccttccg tttttctgaa tgatatttcg ggaacggctt
      601 tccagttaaa catcgattat tttacgccgc ccattgatgc cggggacttt aacgaaatca
      661 aacaaaaagt aaacctggag attttaaaac tcctggaaac cctgaaagtg ccgctttccg
      721 gatcgagcgt ttccgtaaag ctgatggggg aggaaaacaa aaaggaaaaa caggcggatg
      781 cctgaagcgg atcaatgctg tctcatcgct cctgcagccc ggaacagcct tttatttttt
      841 aattccttat ctgatcatcg ccttcatctc aagcaaaaag cctttgacct gttccagttt
      901 tttgatggac tcgaattttt cttctgcttt tttactcttt ctgaggaaat cctttgcccc
      961 ttcgatggtg tatttgcgct ggcggagcag atggtatatc aagtggaggt tcttgatgtc
     1021 gacggggcgg aaataccggt cgcccttctt attctttttt ggctggagaa tgctgaattc
     1081 ggcctcccag aaacgcagga gggattgatt cacccggaac atatccgaga cttctcccat
     1141 cgtgtagtat tgtttgctga aaagctgctc atcatccgga atctggatca gatctgcttc
     1201 ttcggaaatc tgacggatgg attttctgcc ccggcccgac ctgggtttct gaaaagggga
     1261 atccgggttg ctaccagaaa cctgggattc cgaatggccg gtttccggcg cttcaacgga
     1321 aacctcttcc ggttctttat tttcaggata tttaagcttt tttatcctga tcccgatatt
     1381 cattggctta tcatcctgac ccggaggggg gtcctgacga agaaccggtt cttcaataaa
     1441 attcaatgag atctgggaaa aggtatccat gcttgtacca aaaataacaa aaactattcc
     1501 gaaacgatct aaagttttcc gaaaaatcta atcgaagctc tggttactgg cggaggcgag
     1561 tttgagcaaa cggtcatatt gctccggggt aatatcatta taaaaataat acaccggatc
     1621 aaccggctcc ccgttgcgat gtacttcgta gtgacaatgc ggtccggtgc tttttccggt
     1681 actgcccacc caaccgataa tttcaccccg tttcaccttt tcaccggaat gcactttaat
     1741 ccggaacata tgcccgaaca aggtggaata cccgtatcca ttgtgaatga ccacgttgtt
     1801 tccataacca tttccggtat tgccggcagt ttccaccgtt ccatttgccg tggcatagat
     1861 gggcgtgccc aggggtgccg caaaatcaag ccccgcatgg aatttggttg tcttatatac
     1921 cggatccacc cggtagccaa accccgaagc aatgcgtttc aggtccttat tgctcaccgg
     1981 ttgtatcgcc ggggtacagg cctgcaactg ctctttattt ttaatgaagc cgttgatgtc
     2041 cg
//
LOCUS       DASYCU010000004         7830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2030951, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000004 DASYCU010000000
VERSION     DASYCU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2030951"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGM24_00060"
     CDS             <1..187
                     /locus_tag="VGM24_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY4153776.1"
                     /translation="IYVPSNSGLVVAVNRDGSVLWKHKISNALITCILPVSGNKVIAT
                     TMDGKLVCLRYSSGLHF"
     gene            232..1161
                     /locus_tag="VGM24_00065"
     CDS             232..1161
                     /locus_tag="VGM24_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4153777.1"
                     /translation="MKLFFPGLVFLFLLFANIPRIAFSQYKTDKKLESQLRTLVDSFH
                     GTAGIYVRNLKTGKEASINADTIFPTASIVKIPILIGIFNKIQQGAYTYHQPLVYLDS
                     AARGGSGLMQYFKDSTKTDLRTAITLMITHSDNTAAVWCEKLAGGGVSINSWLSDHGF
                     KDTRVNSRVAGREPDNKLYGWGQTTPREMAALLVLIRQGRAVSPEASERMYRILTHIF
                     WDEYALSEIPPYVQAASKQGMVNDSRSEAMLVNAPHGDYVFYVATKNNKDQRWEPDNE
                     AWVLARKLSALLWNYYEPHSGWKPAAGSEKYFH"
     gene            complement(1194..2423)
                     /locus_tag="VGM24_00070"
     CDS             complement(1194..2423)
                     /locus_tag="VGM24_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153778.1"
                     /translation="MKNLIVLLTVIFATASQAQTVTGYWYGQANVEMSGIQNNYLTEL
                     IIAQKGNKVKGFFGYYFKDVYQSFVVKGTYDKASRKVIIHDIPIIFYRTNSTMNSVDC
                     ITSFYGTLRVSRVKTELSGYFYRDKKYTYTCPDLRVNYTLYAEAKVQDSIFKDMASTR
                     SFWKPQPPPVELVKGNTAGTESELEAVPDSDRTAKKEAESSGISAGNKAGSAPSGNKA
                     RRATDGTVTDSTEGPAEARDQAATGNGAKPVSKTGAASVAVVKHNETIPPLAEERPVF
                     PPDIVKINTDFKKRKQILGKLIDVVSDSVRLSFYDNAEVDGDSISVFLNGALVIAHQE
                     LNERAFNVYVKLDSTKDINEVSMFAENLGKYPPNTALMVISDGEKRYETFLSSDFNGN
                     ATIRLRRRRNGIAGSGQ"
     gene            complement(2539..2976)
                     /gene="smpB"
                     /locus_tag="VGM24_00075"
     CDS             complement(2539..2976)
                     /gene="smpB"
                     /locus_tag="VGM24_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY4153779.1"
                     /translation="MTEIRNRAAYHEYQIEEKYDAGIVLAGTEVKSIREGKLSFADSF
                     CIFFKSELWVRNLHIAEYRFATTNNHIAVHDRKLLLNRRELRKLEARLKDKGYTIVPL
                     RVFLSEKGLVKMQIGLGKGKKLHDKRESIKERDTDREIKRYLK"
     gene            complement(3009..3920)
                     /gene="accD"
                     /locus_tag="VGM24_00080"
     CDS             complement(3009..3920)
                     /gene="accD"
                     /locus_tag="VGM24_00080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY4153780.1"
                     /translation="MKREEDFEANLSGEEGTTEETKTNWFKRIKKGILTSTAEKKETP
                     EGLWTKCPGCNYICTMTELRENLFVCPKCNHHHRIGSDEYFEILFNDNKYDVMYENIR
                     SKDFLGFTDLKSYEKRLEETWAKTDITDSIRVAAGQVKDQHMVVACMDFEFIGGSLGS
                     VMGERIARAIDYCLEHRAPLMIICKSGGARMMESAFSLMQLAKVSGKLSQLTDARIPY
                     ISLLTDPSFGGISASFGMLGDLNIAEPGALIGFAGPRVIRETIKKDLPEGFQRSEFLL
                     EHGFLDFIVNRKELKQKIATVLTLLRN"
     gene            4014..4697
                     /locus_tag="VGM24_00085"
     CDS             4014..4697
                     /locus_tag="VGM24_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEY4153781.1"
                     /translation="MKWSEFLTSSIGKKFIMSLTGISLIAFLVVHVGINACIWANDGG
                     ETFNKISHFMGTNIIIRIIEVGLFAGLILHIVQGLVLEVQNRSRRKTGYAIKLGNRGS
                     KWYSRSMGLLGTLLLFFLVIHLAHFWVPSRFGDLPTVDIDGKELANQYAEIVRVFQNP
                     VVDVIYILGCASLLYHLLHGFQSAFRTLGVPNGKYIDLIKGIGYAFAVIVSVAFAMMP
                     VSVYLDWVS"
     gene            4750..6723
                     /locus_tag="VGM24_00090"
     CDS             4750..6723
                     /locus_tag="VGM24_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY4153782.1"
                     /translation="MFDSKTPPGPLGQKWNEYKGHIKLVNPTNKRKIEIIVVGTGLAG
                     ASAAATLGELGYRVKSFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYETIKG
                     GDYRSRESNVHRLAEVSVDIIDQCVAQGVPFAREYGGLLSNRSFGGTQVQRTFYAAGQ
                     TGQQLLLGAYQALERQVALGNVTVYARHEMQELVVIDGKARGIITRNLLTGELERHFG
                     HTVVLCSGGYGNVFYLSTNAMGCNATAIWKAHKKGAYFANPCYTQIHPTCIPVSGDYQ
                     SKLTLMSESLRNDGRIWVPKNQNETRKANEIPEAERDYYLERRYPAFGNLVPRDVASR
                     AAKERCDAGYGVGTSRQAVYLDFAAAIHRYGHVVVHKQRIGNPTEEQIRNFGKEVVKE
                     KYGNLFDMYEKITGENPYEVPMRIYPAIHYTMGGLWVDYELMTSIRGLYCLGEANFSD
                     HGANRLGASALMQGLADGYFVIPYTIGNYLAEEIRTAEIPTDHPAFAEAEKAVASRMD
                     RLMQIKGTKTVDHFHKQLGKIMWDKCGMARNAAGLQEAIGEIRELKKAFYQDLKIPGD
                     IKEMNPELDKANRVADFLELGELMCMDALNRNESCGGHFREEMQTAEGEAKRDDEHFS
                     FVSAWEYKGEHDWELHKEALEFEVAKPSQRSYK"
     gene            6790..7560
                     /locus_tag="VGM24_00095"
     CDS             6790..7560
                     /locus_tag="VGM24_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY4153783.1"
                     /translation="MDHYKMNLTLNVWRQKNNLTAGRFETYKVENISSEMSFLEMMDV
                     LNERLVSEGKEPVAFDHDCREGICGTCSMYINGRPHGPWLANTTCQLHMRAYKDGDVI
                     TIEPWRANSFPVIKDLIVDRSAFDRIIQAGGYISVNTGNAQDANSLPVAKDLSDASFA
                     AAACIGCGACVAACKNSSAMLFVSAKVSHLAMLPQGMPERKSRARNMLAQMDKEGFGS
                     CTNTQACEAVCPKEISMTNIARLNAEYILAGFGERSDG"
     gene            complement(7563..>7830)
                     /locus_tag="VGM24_00100"
     CDS             complement(7563..>7830)
                     /locus_tag="VGM24_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153784.1"
                     /translation="VRKEDIASIRDPQNWILQRKVNYAPVIQTPGSPAKAEIRIMYIW
                     PEGAKRPRPAINLARLSKGKMIGVRYNKDQDWVGGSVALFDQNA"
BASE COUNT         1835 a         1997 c         2010 g         1988 t
ORIGIN      
        1 gatctatgtg ccttccaatt caggactggt cgtggcggta aaccgcgacg gatcggtttt
       61 atggaaacac aagatctcaa atgcactcat tacctgtatc ctgccggtaa gcgggaataa
      121 ggtaatagcc acgacgatgg atgggaaact ggtctgcctg cggtattcgt ccggcctgca
      181 tttctgaatc cgcctatttc ttaaatgcat aacttttcat aaaatcgctg tatgaaatta
      241 ttttttcccg ggcttgtttt tttattcctg ctgtttgcga atattccccg catcgcattc
      301 tcccaatata aaacggataa aaaactggaa agccaactcc ggacgctggt ggactcattt
      361 catggaaccg caggcatcta tgtccgcaat ctgaagaccg gaaaagaagc ttcgataaat
      421 gcagatacca ttttcccgac ggccagcata gtaaagattc ccatcctgat cggcatcttc
      481 aataagatcc agcagggcgc gtacacctat caccagcccc tggtatatct cgattctgca
      541 gccaggggag gatccggcct gatgcaatat ttcaaagaca gcacgaagac cgatttaaga
      601 acggccatca ccctgatgat cacccacagc gataatacgg cagcagtctg gtgcgaaaag
      661 ctggccggcg ggggcgtcag catcaacagc tggctttcgg atcacggctt taaggatacc
      721 cgcgttaact cccgggtggc gggccgcgag ccagataaca agttgtatgg ctggggacag
      781 accacgcccc gggaaatggc cgcgctgctg gtattgatcc ggcagggcag ggctgtcagc
      841 ccggaagcca gcgaacggat gtaccggatc cttacgcata ttttctggga tgaatatgcg
      901 ctttctgaaa ttcctcctta tgttcaggcg gcttccaagc agggcatggt gaatgattcc
      961 cgttctgaag ccatgctggt caatgcgccc cacggggatt acgttttcta tgtggccaca
     1021 aaaaataata aggatcaacg atgggagccg gataatgagg cctgggtgct ggcccgtaag
     1081 ctatcggcgc tgttgtggaa ttattacgaa cctcattccg gatggaaacc ggcagccggt
     1141 tcggaaaaat attttcatta aaggaattcc ttcgcctggt ctttctcccg ggcttattgc
     1201 ccggatccgg caataccgtt tcgccggcgg cgcagacgga tggtcgcgtt cccgttgaaa
     1261 tcgctggaaa gaaaggtctc gtatcttttt tctccgtcac tgatcaccat gagcgccgtg
     1321 ttgggcggat attttcccag gttctccgca aacatgctca cctcgttgat gtccttcgtg
     1381 ctgtcgagtt ttacgtacac attaaaagcc ctttcgttaa gctcctggtg ggcaataacc
     1441 aacgccccgt tgagaaatac ggaaatgctg tctccgtcca cttcggcatt gtcgtaaaag
     1501 ctcagccgca cgctgtcgga gaccacgtca atcagtttgc ccaggatctg ttttctcttt
     1561 ttaaaatcgg tgttgatctt tacgatgtcc ggaggaaata ccggcctttc ttctgccagc
     1621 gggggaatgg tctcattatg ttttaccacc gcaacggatg cggcgccggt tttcgatacc
     1681 ggttttgccc cgtttccggt ggccgcctga tccctggcct ctgccggtcc ttccgtcgaa
     1741 tccgtgaccg tcccatctgt tgctctcctt gctttattcc cggaaggggc ggatcctgct
     1801 ttgttccctg ccgatatccc ggacgattct gcctctttct ttgctgttct atcagaatcc
     1861 ggaacggcct ccagttcgga ttcggttccc gcagtattcc ctttcaccag ttccacagga
     1921 ggcggctgcg gtttccagaa cgagcgcgta ctggccatat ctttaaaaat gctgtcctgt
     1981 accttggcct cggcatagag cgtgtaattt acccgcaggt cggggcaggt atacgtatat
     2041 tttttatccc ggtagaaata accgctgagt tctgtcttta ccctggacac ccggagggtt
     2101 ccgtaaaaac tggtaatgca atccacgctg ttcatggtgg agttcgttct gtaaaatatg
     2161 atcgggatat cgtggatgat caccttgcgg ctggccttgt cgtaagttcc cttgacaacg
     2221 aaactctggt agacatcctt gaaataataa ccgaaaaacc cctttacctt atttcccttc
     2281 tgggcaatga tcagttcggt caggtagttg ttctggatac cggacatttc cacatttgcc
     2341 tgtccatacc aatatccggt aaccgtttgc gcctggcttg ctgtggcgaa aatcactgtt
     2401 aagagaacga tcaggttttt catgggtttc aatgatgggt attcgaactg atctaacgct
     2461 ttaatctgac aatcattata ccattctggg aattttttgg gctttccgct gaaaatgacc
     2521 ggattacccg ggattccgct atttcaggta ccgttttatt tcccggtccg tatccctttc
     2581 cttgatggat tcccgtttat cgtggagttt tttccctttc cccaggccaa tctgcatttt
     2641 aaccaggcct ttttcactaa gaaatacccg gagcggaacg atggtatacc ccttatcctt
     2701 caatctggct tccagtttac gcaactccct gcggttcagc aacaatttgc ggtcatgcac
     2761 ggcaatatgg ttgttcgtgg tggcgaaccg gtattccgct atgtgcaggt tcctgaccca
     2821 tagttcggat ttgaaaaaga tacagaagct gtcggcgaaa ctcagttttc cttcccggat
     2881 ggatttcacc tccgtgcccg ccaatacgat gcccgcatcg tatttttctt cgatctggta
     2941 ttcatgatag gccgctcggt tcctgatttc cgtcatgggg aataaaaatt aggcaggtta
     3001 ggctgatgct aattcctgag gagtgtaagg acggtggcaa tcttttgttt taattccttc
     3061 cggtttacga taaaatccag gaatccgtgt tcgagcagaa attcactgcg ctgaaaacct
     3121 tcgggtaaat ccttttttat ggtctcccgg attacacggg gtccggcaaa accgatcagg
     3181 gcacccggtt cggcaatatt gagatcgccc agcataccga atgatgcaga aatccctccg
     3241 aaagagggat ctgtcaataa ggagatatag ggaatgcggg catccgttaa ctgggaaagc
     3301 tttccggata ccttggccag ctgcattaaa gaaaatgcgc tttccatcat cctggccccg
     3361 ccggatttgc agatgatcat cagcggcgcg cgatgctcca ggcaatagtc gattgccctg
     3421 gcaatgcgtt cgcccatcac acttcccagg gacccgccga tgaattcaaa gtccatacag
     3481 gccaccacca tatgctgatc ttttacctga cccgcggcca cccggatgga atccgtgata
     3541 tcggtcttgg cccaggtttc ctccagcctt ttttcatacg acttcagatc ggtgaacccg
     3601 aggaaatcct ttgaccggat attctcatac atcacatcgt atttgttatc gttgaaaagg
     3661 atctcgaagt attcgtcgct gccgatgcgg tgatgatgat tgcacttggg acaaacaaaa
     3721 agattttcgc gcagttcggt catcgtgcag atatagttgc aacccgggca ctttgtccag
     3781 agaccttcgg gagtttcttt tttctcggcg gtgctggtca aaattccttt tttgatccgc
     3841 ttgaaccaat tcgtttttgt ctcctccgtc gttccttcct cacccgaaag attggcctca
     3901 aaatcttctt cgcgcttcat caaaaacaga tttttgagcg gctaagatag ggatttcccg
     3961 tttcctgtct tatttttgcc atattctact gattctcaat ttttcaagac aatatgaagt
     4021 ggtctgaatt tttaacctcg tccatcggca agaagtttat tatgtccctg accggaattt
     4081 ccctgatcgc cttcctggtg gttcatgtgg gcatcaatgc ctgcatctgg gccaatgacg
     4141 gaggagagac gtttaacaag atttcccatt ttatgggaac caacatcatc atccgtatta
     4201 tcgaagtcgg attgtttgcc ggcctgatcc tgcatatagt tcaggggttg gtcctggaag
     4261 tgcagaaccg cagccgccgc aaaaccggct acgccatcaa gctcggcaac cggggcagca
     4321 aatggtacag ccgcagcatg ggtttgctgg gcaccctgct cctgtttttt ctggtcatcc
     4381 acctggcgca tttctgggta ccctcgcgtt tcggggatct tcccacggtg gatatcgacg
     4441 gaaaagagct ggccaaccag tacgctgaaa tcgtgcgcgt gttccagaac ccggtggtgg
     4501 acgtgatcta tattctcggt tgtgcttccc tgttatacca cctgttgcac ggatttcaga
     4561 gcgcgttcag aaccctcggc gttcccaacg ggaagtacat cgatctgatc aaagggatcg
     4621 gatacgcctt tgcggtgatc gtttccgttg cttttgccat gatgccggtt tctgtttacc
     4681 tggattgggt gagttaggga atttccggat cgtcaaatta tctgaatatc aaattgtcta
     4741 attaactata tgttcgattc aaaaactccc ccggggccgt tagggcaaaa atggaatgaa
     4801 tacaaaggac atattaaact cgtgaacccc accaacaagc ggaagatcga aattatcgtg
     4861 gtgggaacag gtcttgccgg cgcttctgcc gccgcaacgc tcggcgaact gggttaccgg
     4921 gtgaagtcgt tctgtttcca ggattccccg cgtcgcgcac acagcattgc cgcccagggg
     4981 ggaatcaacg cggcaaaaaa ttatcagaac gacggggata gtgtttatcg cctgttttat
     5041 gaaaccatca aaggcggcga ctaccgctcc cgcgaatcca atgttcaccg gctggccgaa
     5101 gtcagtgtcg atatcattga ccagtgcgtt gcccagggtg ttccttttgc gcgggaatac
     5161 ggcggcctgc tgagtaaccg gtcttttggc ggcacgcagg ttcaaagaac cttttatgcg
     5221 gccgggcaaa ccggtcagca actgctgctc ggcgcctatc aggccctcga gcgccaggtg
     5281 gctctgggta atgtgaccgt atatgcccgt catgaaatgc aggaactggt ggtgattgac
     5341 ggtaaagcaa ggggaatcat tacgcgcaac ctgctcaccg gagaactgga aagacatttc
     5401 ggtcataccg tggtcctttg ttccggcggg tacggcaatg tattttatct ttccaccaat
     5461 gccatgggct gtaacgccac cgccatatgg aaagcacata aaaagggagc ttattttgcc
     5521 aatccctgct atacccagat tcatccgacc tgcataccgg tttccggaga ttaccagtcg
     5581 aagctgaccc tgatgtccga atccctgcgt aacgacggcc ggatctgggt tcctaaaaat
     5641 caaaatgaaa cgcgtaaggc aaacgagatc ccggaagcgg aaagggatta ttatctggaa
     5701 agaaggtatc cggctttcgg aaacctggtt ccgagggatg tcgccagccg cgccgccaaa
     5761 gaaagatgcg acgccggtta cggagtgggc acttcccgcc aggcggtata cctggatttc
     5821 gcagcggcca ttcaccgcta cggccacgtg gtggtccaca aacaaaggat cggaaatccg
     5881 acggaagaac agatcagaaa tttcggaaaa gaagtagtga aagaaaaata tggtaacctt
     5941 ttcgatatgt atgaaaagat taccggagaa aatccgtacg aagtgcctat gcggatctat
     6001 ccggcgattc attataccat gggagggctc tgggtggatt atgaactgat gaccagcatc
     6061 cgggggctgt attgcctggg cgaagcgaat ttctccgatc acggagcaaa ccggctggga
     6121 gcatccgccc tgatgcaggg acttgcagac ggctactttg tgattcccta tacgatcggg
     6181 aactacctgg ccgaggagat ccgcacagcg gagatcccaa cggatcatcc ggcttttgca
     6241 gaggcggaga aagcagtggc ttcccgtatg gaccggctga tgcagatcaa aggaacaaaa
     6301 accgtggatc attttcataa gcagctgggg aagatcatgt gggataaatg cgggatggcg
     6361 cggaacgcag ccggactgca ggaagccatc ggtgaaatcc gcgagctcaa aaaggcgttt
     6421 taccaggacc tgaaaattcc gggtgatata aaggagatga accccgaact ggataaagcc
     6481 aaccgcgtag ccgactttct cgagctgggg gaattgatgt gcatggacgc gctgaaccgg
     6541 aatgaaagct gcggcggtca tttccgggaa gaaatgcaaa ccgcggaagg cgaagccaaa
     6601 cgcgatgacg agcatttcag ttttgtttcg gcatgggaat ataaaggaga gcatgattgg
     6661 gaactgcaca aggaagcgtt ggaatttgag gtggcgaagc cctctcaacg cagttataag
     6721 tgatggcaat ggaaacggat tccgggagga caattttatt tataatcgga aaagaaatta
     6781 atataatcca tggatcatta taaaatgaac ctgacgctca acgtgtggcg ccagaaaaat
     6841 aatcttacag cgggtaggtt cgaaacatat aaagtggaaa atatttcctc ggagatgtcc
     6901 ttcctggaaa tgatggatgt gctgaatgag cgcctggtga gcgaagggaa ggagcccgtg
     6961 gctttcgatc atgactgccg ggaaggaatc tgcggcacct gttccatgta tatcaacggc
     7021 cgcccccacg gcccctggct ggccaatacc acctgccagc tccatatgcg ggcatataag
     7081 gacggagatg ttattacgat tgaaccctgg agagccaatt cctttccggt gataaaggac
     7141 ctgatcgtgg accgctctgc ctttgaccgg atcatccagg ccggcggata tatttcagtg
     7201 aataccggga acgcccagga cgccaattcc ctgccggtag cgaaagacct ttccgacgcc
     7261 tcctttgccg cagcagcatg catcggttgc ggagcctgcg tggccgcctg caagaacagt
     7321 tcggctatgc tttttgtctc ggcaaaagtt tcccatctgg ccatgctgcc ccagggcatg
     7381 ccggaaagaa aatcccgcgc ccgcaatatg ctggcgcaga tggacaagga aggattcggc
     7441 agctgcacca atacccaggc ctgtgaagcg gtttgtccga aggaaatctc catgacgaat
     7501 atcgcccggc tgaatgcaga atatattctg gcaggtttcg gcgaaaggtc tgatggctga
     7561 ccttatgcgt tctgatcaaa tagcgccacg cttcccccga cccaatcctg gtctttattg
     7621 taccgcacgc cgatcatttt ccccttgctt agcctggcca ggttgatggc cggcctgggt
     7681 cgttttgctc cttccggcca gatatacatg atccggatct ctgccttggc cggactgccc
     7741 ggcgtttgaa tcaccggcgc atagttcact ttgcgctgca ggatccagtt ttgcgggtcc
     7801 ctgatggacg cgatatcttc ctttcttaca
//
LOCUS       DASYCU010000005         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1741323, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000005 DASYCU010000000
VERSION     DASYCU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741323"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /gene="pheT"
                     /locus_tag="VGM24_00105"
     CDS             complement(<1..1457)
                     /gene="pheT"
                     /locus_tag="VGM24_00105"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY4153785.1"
                     /translation="MTISYHWLCDYLPDHPVKPSPEQLSKILTSIGLEVESLTRYESV
                     KGLLDGLVVGEVISCEPHPDADKLKLTRVDTGQKESLQIVCGAPNVATGQKVVVAPAG
                     TTLYPETGEPLRIKTTKIRGVESQGMLCAEDEMGLGKSHAGILVLPKNLAPGSAVADY
                     LKPYTDFIFEIGLTPNHMDAMSHIGVAREVCAYLSHQDNKEVRVKMPVTPVFSTDNRN
                     PPVKINIENRKACARYSGVSIRGIRIQDSPKWMQDKLRAIGIRPINNVVDITNFVLQE
                     MGQPLHAYDISQIKGNEIHVKNLPAGTAFITLDGKERKLDAEDLMICDAESPLCIAGV
                     FGGLNSGIGNNSIGLFLESAWFNPSGIRRTSFRHGLRTEAALHFEKGMDISGTVQALK
                     RAAALIREYAGGKIDTEIADLYPEPFPRNQILLKYHYLKKLSGKNYHSVTVKNILLSL
                     GFDILREGLDDLLVAAPHHKPDIQLPADIVEEIMR"
     gene            1671..>2509
                     /locus_tag="VGM24_00110"
     CDS             1671..>2509
                     /locus_tag="VGM24_00110"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEY4153786.1"
                     /translation="MKNITLIFFAAAFSFASCGPARVVETTSYDPAPEAAPPIVSYQS
                     FYDGLAPYGSWIDYPGYGYVWMPNAGPDFMPYNTNGNWIYTDAGWTWASNYEWGWAPF
                     HYGRWFYEGGYGWMWVPGSEWAPAWVSWRGGNDYYGWAPLAPDVSVSVALGSYDPPVN
                     YWNFVPRQYMGNPGWHSHYVDHSRNVTIINNTTIINNYSGTNRARYAFAPGPDPNEVR
                     RVSGTNIQPVRIREASRPGSRVSGNEFDIYRPRVDAAPATRGGAATRPAPARAQNFRD
                     LRP"
BASE COUNT          556 a          632 c          678 g          643 t
ORIGIN      
        1 atccgcatga tctcttccac gatatcggcc ggcagctgga tgtccggttt gtgatgcggt
       61 gcggcgacca gcaggtcatc cagcccctcc cggagaatat cgaagcccag ggaaagcaat
      121 atgttcttaa cggttaccga atgatagttt ttcccgctca attttttcag gtaatggtat
      181 ttaagcagga tctggttcct gggaaagggc tccgggtaaa ggtctgcgat ctccgtatct
      241 atttttcctc ccgcatattc ccggatgagg gcagcggctc tcttcagggc ctgaaccgtt
      301 cccgagatat ccatcccctt ttcaaagtga agggccgcct cggtgcggag gccgtgacgg
      361 aaagaagttc ttctgatacc ggagggattg aaccaggcgc tttccaaaaa cagcccgatg
      421 ctgttattgc cgattccgct gttcaacccg ccgaatacgc ccgcgataca tagcggggat
      481 tccgcgtcgc agatcatcag gtcttcggca tccagttttc tttctttacc gtcgagggta
      541 ataaaagcgg tcccggcggg aaggtttttg acgtggatct cattgccttt gatctgggaa
      601 atatcatacg catgcagggg ctgccccatt tcctgtaaaa cgaaattggt gatgtcgact
      661 acattattga tcggacgaat gccgatggcc cggagtttat cctgcatcca ttttggagaa
      721 tcctgtatcc ggatgccgcg aatgctgacg cctgaatagc gggcgcaggc tttccggttt
      781 tcgatattta tttttacggg cgggttccgg ttatcggttg aaaagaccgg ggtcaccggc
      841 attttcaccc gaacctcttt attatcctga tgtgataggt aggcgcaaac ttcccgggcg
      901 acgccgatat ggctcatggc gtccatatgg ttgggggtaa gaccgatctc gaatataaaa
      961 tccgtatagg gtttcaggta gtcggccact gctgagccgg gagcaagatt tttcgggaga
     1021 acaagaatgc cggcgtggct tttccccagc cccatctcgt cttcagcaca aagcattccc
     1081 tggctttcca caccgcggat ctttgttgtc tttatccgga gaggctcgcc ggtttccgga
     1141 tataaagtgg ttcccgccgg agccaccacc actttttgcc cggtggccac attgggcgct
     1201 ccgcaaacaa tctgaaggct ttccttttgc ccggtatcta cccgggttaa ttttagtttg
     1261 tcggcatcgg gatgaggctc gcaggaaata acctctccaa ccaccaggcc atccaggaga
     1321 ccttttaccg attcatagcg ggtcaggctt tccacttcca gtccgattga agtcagtatt
     1381 ttggaaagtt gttcaggcga aggttttacc ggatgatcgg gaagatagtc gcaaagccag
     1441 tggtagctga tggtcatgga aagagagcga tttttttaga acggtaaaga tagtttttat
     1501 ggcgtttcgc cggccttccc cagtcaaaag agccccgagt gccctgtatg gcaggatttt
     1561 agatagaaca gcgtactata attatttgtt aatcgtttgt aaataaatat acttttagct
     1621 tccaccggaa tcaaagagac ggactattta acccaataaa tgttagcgtc atgaaaaaca
     1681 ttacactgat ttttttcgca gctgcttttt cttttgcctc ctgcgggccg gccagggtgg
     1741 ttgagacaac ttcttatgat cccgcacctg aagcggcgcc gccaatcgtt tcttaccagt
     1801 ctttttatga tgggctggct ccttatggca gctggatcga ttatcccggt tatggatatg
     1861 tgtggatgcc gaatgcggga ccggatttca tgccatacaa tacaaacggc aactggattt
     1921 atacggacgc cggatggacc tgggcttcaa attacgaatg gggttgggct cccttccatt
     1981 atggccgctg gttttatgaa ggcgggtatg ggtggatgtg ggttcccgga agcgaatggg
     2041 cgccggcctg ggtaagctgg agaggcggaa acgattatta cggatgggct ccgctggccc
     2101 cggatgtttc tgtcagcgta gcactgggtt cttatgaccc tccggtgaat tactggaatt
     2161 ttgttccccg tcagtatatg ggaaatccgg gatggcacag tcactatgtg gatcattcgc
     2221 gcaacgtgac cattatcaac aataccacca ttataaataa ttacagcgga accaaccggg
     2281 ccaggtatgc ttttgctccg ggccctgatc cgaatgaagt gagacgggtt agcggaacaa
     2341 atattcagcc ggtccgcatc cgggaagcca gccgcccggg aagccgggta tccggaaatg
     2401 aatttgacat ctaccggccc cgggtagacg cagctcccgc cacccgcgga ggtgcagcga
     2461 ccaggccggc cccggcaagg gcgcaaaact tccgggacct gcgacccca
//
LOCUS       DASYCU010000006         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4350534, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000006 DASYCU010000000
VERSION     DASYCU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4350534"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VGM24_00115"
     CDS             complement(<1..1098)
                     /locus_tag="VGM24_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4153787.1"
                     /translation="MSAFGKNARTGLTGFKNTGRHLKIISSLLLAACLQLPANAYSQG
                     ISLSVKNASLDTVFARIQKQSPYRFIFTSEELQGTAKLSFTITEAGIREILHLCFKDQ
                     PLTYSMEDIYIIVKRKEKKENKQEEKGAEINVGGKVINEDGEGLAAVTVMNEDQTLGT
                     ASNEQGFFELKGVPADAVLLISSMGYEQQRLPLNGRTNLLLQLKTAANNLDELIVKGY
                     YSTSRRLNTGSVTRVSSEQIATQPVSNPVLALQGLVPGLLITQDNGLPGSRFTTLIRG
                     QNSIQSGNSPLYIIDGVPFLSDNESLTQLNGILANSPFNSIDPADIESIEVLKDADAT
                     SIYGSRGANGVILITTRKAKTGKSALSLNAYQ"
     gene            complement(1236..2435)
                     /locus_tag="VGM24_00120"
     CDS             complement(1236..2435)
                     /locus_tag="VGM24_00120"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY4153788.1"
                     /translation="MNGEQSIGENPEDEQAYRIAYLIAGYIREDLTKAEHTELDDWVT
                     ASLENQRLFEEMTDEKNLNAWLKWKEGLPEKEVLMRLKKRLDFTRHRKEPLVRSVWPY
                     AAAACILLVVFVLWRHQPAATKEENQAPLVAQDLGPGTDRATLTLADGKTIRLDSAEN
                     GHLLTMGNIRIDKQDRAQLNYEMINPALRAVKEADNILSTPSGGQFKIMLPDGTRVWL
                     NSGSSLKYPVSFAGSKRSVILSGEAYFEVAKDPSSPFEVKANGSLVEVLGTHFNINAY
                     TDELDLKITLAEGSVKVNHNTLIKPGEQALVNPSGSVRVVQADLQMELAWKNGLFLFK
                     QTELDGLMRQVSRWYDCEVVFQTRVSEHFNASISRDVPASKLLHLLEETGTVHFRIGN
                     KKITVMK"
     gene            complement(2593..3183)
                     /locus_tag="VGM24_00125"
     CDS             complement(2593..3183)
                     /locus_tag="VGM24_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY4153789.1"
                     /translation="MKKKSISPEAGLMEGFRQGRESAFTELFTSLYPALCFYALKITN
                     DQAAAEDIAEESFIRVWEKREIFFQFSVLKSYLYTTVRNAGINWLYENRKYRNLQNEL
                     GQNTRLSEGNPLELIIHAELFREMYSALDKLPPKCRQIVTMLFIEGKKVKDVARELQL
                     STGTIQTQKTRGLMLLRKYPLNFLLLLGSFLYSSGV"
     gene            complement(3350..>4522)
                     /locus_tag="VGM24_00130"
     CDS             complement(3350..>4522)
                     /locus_tag="VGM24_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY4153790.1"
                     /translation="GLVGNPLQRAKADSQMLAGMQQIADARMRAGWYVKDGVLIFSGK
                     GDNLCTEKKYGDFEMFVDWKINKDGDAGIYLRGSPQVQIWDTSRVDVGAQVGSGGLYN
                     NQVNPSKPLKLADNAIGEWNNFDIIMKGDRVTVYLNGVLVVDNTILENYWDRNLPIFP
                     EEQIELQAHGNEVSYRDIYIKEIPRQQPVTLSEEEKKDGFRMLFDGTNMHEWTGNTSG
                     YTMEDGDIVVRPEHGEHGNLFTKEEYSDFIYRFEFQLTPGANNGIGIRAPLEGDIAYT
                     GMEIQVLDNEAPIYKDLHPYQYHGSVYGVIPAKRGYLKPTGEWNSEEITAIGNKIKVV
                     LNGTVILDGDIAEASKNGTMDGKEHPGLKRTGGHIAFLGHGDVLRFRNVRVKSLSN"
BASE COUNT         1040 a         1181 c         1043 g         1258 t
ORIGIN      
        1 ctggtatgca tttaaggaaa gtgcgctttt cccggttttt gccttccggg ttgtaataag
       61 gataacgccg tttgcgcctc ttgacccata gatggaggta gcatctgcgt ctttgagaac
      121 ctcgatactt tcaatgtccg ccgggtcaat ggaattaaac gggctgttgg caagtatgcc
      181 gtttaactgg gtcagcgatt cgttgtctga caaaaaagga actccgtcaa taatatacag
      241 ggggctgttt ccgctctgaa tggagttctg accccgtatg agggtggtaa accgggatcc
      301 cggcaggccg ttgtcctgcg tgatcagcaa tccgggcacc aggccctgca aagccagtac
      361 gggattggat acgggttgtg tagcgatctg ttccgaagag acccgggtta cgcttccggt
      421 atttaagcga cgtgaagtgc tgtaatatcc tttcacgatc aactcatcca ggttgttggc
      481 agccgttttc aactgcagga gcaaattcgt tctgccgttc aacggcagcc gctgttgttc
      541 atatcccata ctgcttatca gcagaaccgc atcggccggc actcctttta attcaaaaaa
      601 cccctgttca ttgctggcag tacccagggt ctggtcctca ttcatcaccg taacggcagc
      661 cagtccctcc ccgtcttcgt tgatcacttt cccgccaaca ttgatctcag cccctttctc
      721 ctcctgttta ttctctttct tttctttcct ctttacgatt atataaatat cctccatgga
      781 ataagtgagc ggctgatctt tgaaacagag atggagaatc tcccggatac cggcttccgt
      841 gatggtaaaa ctcagttttg cggtaccctg cagttcctca ctggtgaaga tgaaccggta
      901 gggagattgt ttctgaattc tcgcaaatac cgtatccagc gaagcattct ttaccgagag
      961 gctgatgccc tgggaatagg cgtttgccgg aagctggagg caggccgcca acaggaggga
     1021 gctgattatt ttcagatggc gcccggtatt tttgaacccc gttaatccgg tacgcgcatt
     1081 tttgccaaat gcactcaaaa gcatagtttt ggttgtttgg gtaaatgaat ggttggtccc
     1141 gcaaaagaat tcatccagga attaactcaa gcttttggcc gggtggtgtc gcaagcactt
     1201 cccggttttt tgttggaccc ggagatgatt tctgattatt tcataacagt tatttttttg
     1261 ttcccgatcc ggaaatgaac agtcccggtt tcttccagca ggtgaagcag tttcgatgca
     1321 ggcacatccc gggatatgct ggcattgaag tgttcggaaa cgcgggtctg aaaaacaacc
     1381 tcgcaatcat accagcgcga tacctgccgc atgagtccgt ccagctcagt ctgcttaaag
     1441 aggaataatc cgtttttcca ggcgagctcc atctgcaggt cggcctgaac tacgcggact
     1501 gaaccggatg gattgaccag cgcctgttcc cccggtttga tcagggtatt atggtttacc
     1561 ttcaccgaac cttccgccag cgttattttg agatccagtt catccgtata tgcatttata
     1621 ttaaaatggg tcccgagtac ttccaccaac gatccgtttg cttttacttc aaacggactc
     1681 gacggatctt tggccacttc gaaataagct tccccgctga ggatcacgct ccgcttgctg
     1741 ccggcaaagg aaacgggata tttcagggaa gaacccgaat tcagccagac ccgggtgcca
     1801 tccggcagca ttattttaaa ctggcctccg gaaggagtgg atagaatatt gtctgcctct
     1861 ttaaccgccc gcagggccgg attgatcatc tcatagttca gctgggcccg gtcctgcttg
     1921 tcaatcctga tattacccat ggttagcaga tggccatttt cagcactgtc cagccggatg
     1981 gtcttcccat ccgccagggt tagcgttgcc cggtctgttc ccggtccgag atcctgtgca
     2041 accaggggcg cctggttttc ctctttcgtt gctgcgggtt gatgtctcca taatacaaat
     2101 actactaaca gtatacaggc tgctgccgca taaggccaga ccgaccgcac taagggttct
     2161 ttacgatgac gggtaaaatc caggcgtttc ttaagtcgca ttaaaacctc tttttccggc
     2221 aacccctctt tccattttaa ccaggcattc agattttttt catcggtcat ttcttcaaaa
     2281 agacgctgat tttccaggct ggccgtcacc cagtcgtcca gctctgtatg ctccgccttc
     2341 gtcaggtcct cccggatata tccggcgatc aggtaagcga tgcggtatgc ctgctcatcc
     2401 tccggatttt ctcctatgga ttgttcccca ttcattctat taagttaaag agaattcggc
     2461 aaaatggatt tcaacccgta agaccgggaa acggggaaaa agagagaaag gatttcaata
     2521 tttttaattc cgccaggaag aagaccggat cacgggtttt taatttcccg ggggaatggg
     2581 aggccagccg gattaaacgc ctgaactgta cagaaatgaa ccaagcagga gcagaaagtt
     2641 taacgggtat ttccttaaga gcataagccc cctggttttc tgggtctgga tggttccggt
     2701 cgaaagctgc aattcccggg ctacatcttt tacttttttg ccttcaataa ataacatggt
     2761 cacaatctgg cggcatttcg gaggcaattt atccagggct gaatacattt cccggaacag
     2821 ttccgcgtgg atgatcagtt ccagcggatt gccttcactc aaacgggtat tttgtcccaa
     2881 ttcattttga aggttgcgat atttcctgtt ctcgtataac cagttaatgc ctgcatttct
     2941 taccgtcgta tataaataag atttcaaaac actgaactga aagaatattt cccttttttc
     3001 ccagacccgg ataaaagatt cttcggcaat atcctcggct gccgcctggt cattcgtaat
     3061 tttcaaagca tagaaacaca gcgccggata aaggctcgtg aagagctcgg taaaggcgga
     3121 ttcccgcccc tggcggaaac cttccatgag tcccgcctcc ggagatatgc ttttcttttt
     3181 catccaacct tattgaagaa ccatcgggtt catcacacaa gataatatat tctcctatac
     3241 ctgtatagga tgaaaaaaat atattccccg ggttatttaa ccctgagctt aggggggaat
     3301 accggaagca tgcatcccgg ccctgtcgaa taaaatgatt tttatgcttc taattggaaa
     3361 gcgatttaac ccgcacattg cgaaaacgaa gcacatcccc atggcccagg aaagcaatat
     3421 gtccgccggt tcttttcagg cccggatgtt ccttcccatc catagttccg tttttggagg
     3481 cttcggcaat atccccgtcg aggataacgg ttccgtttaa taccactttt attttattcc
     3541 cgatcgccgt aatttcttcg gaattccatt ctcccgtcgg tttcaggtaa ccccgcttgg
     3601 ccgggatgac cccgtaaaca gagccgtggt actggtatgg atgcaggtct ttgtatatcg
     3661 gcgcttcatt atccagcacc tgaatttcca taccggtata ggcgatatca ccttccagcg
     3721 gagcacggat accgatacca ttgttggcgc ccggcgtcag ctgaaattcg aaccggtaaa
     3781 taaaatcgct gtattcctct ttagtaaaca ggttaccgtg ttcgccgtgt tcgggccgta
     3841 caacaatatc gccgtcttcc atcgtgtatc cggaggtatt ccctgtccat tcatgcatat
     3901 tggttccgtc aaacaacatc ctgaatccgt cctttttttc ctcctcactc agcgtcacgg
     3961 gctgctggcg gggaatttct tttatataga tatcgcgata ggacacttcg ttgccgtgcg
     4021 cctgcaattc gatctgttct tccggaaata tcggaagatt ccggtcccag tagttttcca
     4081 gaatcgtatt gtccactacc agtacgccat tgagatagac ggtaacccgg tcgcctttca
     4141 tgataatatc aaagttgttc cattccccaa tggcattatc cgccagcttt aacggcttgc
     4201 tgggattcac ctggttgtta tacagaccgc cggaaccaac ctgggcaccc acgtccactc
     4261 tggaagtgtc ccagatctgc acctgcggag aaccgcgcaa atagatgccg gcatccccgt
     4321 ccttatttat tttccagtca acgaacattt caaaatcgcc gtactttttt tccgtgcaga
     4381 gattgtctcc ttttccgctg aagatcagca ccccgtcttt tacataccag cccgcccgca
     4441 tgcgcgcgtc cgcaatctgc tgcataccgg ccagcatctg ggaatcggct tttgcgcgtt
     4501 gcagcggatt tccgacaagg cc
//
LOCUS       DASYCU010000007         7485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3770804, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000007 DASYCU010000000
VERSION     DASYCU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3770804"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VGM24_00135"
     CDS             <1..607
                     /locus_tag="VGM24_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEY4153791.1"
                     /translation="IPDVVSEAYSAQPPKPVVVTEPWYEFVEGNPTGRDIRFGAWTAI
                     LSGAAGHTYGGGHVWLADVPESPAEGGSWPLEKGFDRNTLNYEGAVSMSILSGFFRKL
                     EWWKMAPHPELISEYPDKYCLASPGLEYIIYLRWGGTCKLNLLSSRKEDEFEYYWFNP
                     ATGKESGHSTVKGGAVRFFSSPGGYPGSVHFSDWVLYLHKK"
     gene            complement(746..1198)
                     /locus_tag="VGM24_00140"
     CDS             complement(746..1198)
                     /locus_tag="VGM24_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY4153792.1"
                     /translation="MFNNKSKSEDPEASIPSGSASLIGSGTSMKGDITSSGDLRIDGT
                     LVGNIICSAKVIIGAGGVVEGDVSGQQADIMGKVNGTIKVKELLQLKSNCQVNGNLYS
                     AKLQIEPAATFNGQCHMTAEEPKNLNSNKTSSELRKDGFQKPPSLAIS"
     gene            complement(1231..1626)
                     /locus_tag="VGM24_00145"
     CDS             complement(1231..1626)
                     /locus_tag="VGM24_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153793.1"
                     /translation="MAKFDANSTLKRLQNRYRLVVMNDDTYEEVVTFKLSRLSVYVFL
                     STVFVLLTGLTVALIVFTPLKLYIPGYGDMNNTKELRELKIRTDSLEQEMIYRDQYFQ
                     SLRNVLQGSSSVKTDTTTLKIPKTEKIDD"
     gene            1762..4737
                     /locus_tag="VGM24_00150"
     CDS             1762..4737
                     /locus_tag="VGM24_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153794.1"
                     /translation="MKRIVTTVIIFSFIFFLNGFGQEGVTYDLKKPARYENRTLGYEK
                     TTQTKFKTPRRFLQNTITHYNFYYNTNNKLNEVVARAKANFQDNFTKLLPFYNYNLEI
                     TAHDKRNLDSVIDKVNTAILIRDLRNDWADNLYMLMGQAYYYKNNLDSAYVLFQFVNY
                     AFAPREKDGYPIPIGSNANADEGGNAFIISTNEKRNIVKRTFSLPPSRNEAFIWQTRT
                     FLMQGDLTRASVLIDMLKQDPNFPERLQTSLHELQALLYYRQSNYDSAAYHLDLALDN
                     AVTQAERARWEYLIGQLYERSNHNIAAKRYFEKSASHTLDPVMGVYARLNAIRQNKGN
                     AAGDDYIRKNLEALHRMTRKELFAPYRDIIYYAAGEMELERNNTAAAKAYFKKAIANS
                     GIEGTMRDQAFLKLGWLYLDEKKYPEAKSAYDSVSAADQLISDSVQILMDRKLALAKV
                     VRQMVIIESQDSLQRIAAMTEAERTAYIKKMIRAYRKQRGLSEEGSSAENGNQFRTNA
                     ANADMFNDNNSGEWYFNNPSLKSRGYNDFHSKWGSRPNVDNWQLQSLINKQKPGVAAG
                     RLNNGNGAEAGAAAPAPAELTPEGLLQQVPLTPEKLKRSQDSVENALYALGKALQDLI
                     PDYRSAILTYDSLEKRFPSTRYYQEALFNTYYCYVKLNDAENAARILALMKQKFPTGK
                     YVAIIENPHPVAPDQEIRNNATQNYEKIYQELIEGHFELALTEKKKQDSIYGDRYWTP
                     QLLYVEALYYMHTRHDSLAKATLNHIISTYTGTGMDDKAKNTLRVLNEREKIEEYLTN
                     LKVTRAKDDSLGLADNGKDQTKTATADTAAAKPVAKLKVDSARVSNNKPDSLQAKKPA
                     IFASAFTAAPARPHAVALIMDKVDPVYVTESKNAFDRYNRENFYGKHFDINIVALSDS
                     IKIVLINGFENADAALAYLDKTGKLAGREILPWLPANKYSFIIIDDQNLAVLQTNKDI
                     QAYKKFLSVYFPGKFPPLK"
     gene            4782..7184
                     /locus_tag="VGM24_00155"
     CDS             4782..7184
                     /locus_tag="VGM24_00155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY4153795.1"
                     /translation="MQKANPIFWKIFFGGLGLFIILLLLINFGAFGPLPSLKELENPS
                     IMLASEVYADDGTLMGKYYKDKSNRSNVEYRDISKEAIHALLATEDKRFYEHSGIDGM
                     GVLRAVVKLGHDGGGSTITQQLAKYMLGQGSKNMLRRGIEKLKEWITSVRLERNFTKE
                     EILALYLNAVPFGDNVYGIRNASRTFFQKEPDRLTVDEAAMLIGMLSGNTLYNPRRNP
                     KAAIERRNTVLNRMVESGYLGEAEATKLKQKPIDLSNYKKLDENNGLAPYFRDVIRED
                     LKKWCKEHKNPATGEPYDLYEDGLRIYTTINPRMQIYADEAVAKQMPMLQKALSAQAS
                     IRKGLVWKDHQNILESLIRNSERWQAMEEDGIPVAEIRKSFDQPVQMKVFAWNVKREK
                     DTVMTPLDSIKYNHQMLQTGFMAMDPMSGAVKAWVGGIDFKTYKFDHVNINTKRQVGS
                     SIKPLLYSDAIEDAGFTPETPCENEAQYFDGYGLVPAGGRCGGNGAVVSMATALAYSL
                     NCASAFIMKQVTPKRFVSFLKNINISSKLEPYPSICLGAVEISLYEMLWGYTMFPSGG
                     FSTKPYYLSRIEDKNGNVLDRFDTERKEVISQNTAYTMCRMMQGTVDFGTALGLRDRL
                     GISEMGGKTGTTNDNSDAWFMGYTPQILAGAWIGCDDRFIRLENGLGQGAEAARPIWE
                     YFMAKTLADKDLGLSRKAKFVQPENMKSDMMYDYMNIIDKTPPPGAEGENQGNGNANQ
                     YLDTSSPSIPIDSKLSSDEQNVLKEANAPARKPDASKTENPPPEKKKKGFFRRLFDRK
                     DK"
BASE COUNT         2113 a         1843 c         1858 g         1671 t
ORIGIN      
        1 gatccccgat gtggtcagtg aagcctattc cgcccaaccg ccaaaaccgg ttgttgtaac
       61 ggagccctgg tatgagttcg tggagggaaa tcctaccggg cgggacatcc ggtttggcgc
      121 atggacagct attctgtccg gtgcggcggg tcatacctac ggaggaggcc acgtgtggct
      181 ggcggatgtg ccggaatctc ccgcagaagg cggctcatgg ccattggaaa agggctttga
      241 ccggaatacg cttaattatg aaggcgccgt ttccatgagc attctttccg gcttcttcag
      301 aaaactggaa tggtggaaaa tggcgcctca tccggaactg atttcagagt atccggataa
      361 atattgcctg gcttctcccg gcctggaata tattatttac ctgcgatggg gaggaacctg
      421 caaactgaac ctgctctctt cccggaagga agacgaattt gaatactact ggtttaaccc
      481 ggcaacagga aaggaaagcg gccattcaac cgtaaaaggc ggagctgtcc ggtttttctc
      541 atcaccgggc ggctatccgg gctccgtaca tttcagtgac tgggtcctct atctccataa
      601 aaaataagtc cgggatcagc aggcacacaa gtcctcagcc ttctcacggg accggctaaa
      661 cgccggcaat agaaggccgg ccccggcatt tttcaaatgc ccggattcag gaaaagcaat
      721 aaatcaggga gcgggcaatg cccgttcacg aaatcgccag ggaaggaggc ttctggaatc
      781 cgtctttacg aagttccgaa ctggttttgt tcgaattcag gttcttcggt tcttccgctg
      841 tcatgtgaca ctgcccgtta aaagtggccg ccggttcaat ctgcaatttc gcggaataca
      901 gattgccgtt cacctggcag ttacttttta actggagaag ttccttcacc ttgatggttc
      961 cgtttacttt gcccatgatg tcggcctgct ggccgctcac gtccccttcc accactccgc
     1021 cggcgccgat aatcactttg gccgaacaga tgatgttgcc taccagggtg ccgtcgatgc
     1081 gcaggtcccc gctgctggtg atgtcgcctt tcatggaagt accgctgccg ataagacttg
     1141 cactgccgga gggaatggaa gcttccggat cttcagactt tgatttgtta ttgaacattg
     1201 gtatcagatt ttaaaaaagg atatcagacg ctaatcgtct attttctctg ttttgggaat
     1261 tttcagcgtg gtcgtgtccg ttttcactga actgctcccc tgcagcacgt tccgcaaact
     1321 ctgaaaatac tggtcccggt agatcatttc ctgctctaac gaatcagtcc gtatttttag
     1381 ttcacgaagt tccttggtat tattcatatc gccgtatccg ggaatataaa gcttaagtgg
     1441 agtaaataca attaaagcca ctgtaagtcc ggttaatagc acaaaaaccg tgctgagaaa
     1501 aacatatacg ctaagcctgg aaagtttaaa ggtcacgacc tcttcgtagg tgtcgtcatt
     1561 catgaccaca agccggtatc tgttctggag acgctttaac gtggaattgg catcgaattt
     1621 ggccatatgg acatattgcg gactaaagat aacaagttat tggtttgcag gttaaaacag
     1681 ccttaaaatc agtcattttt aaaataaaaa acgggatttt cagttaataa ccgattagtt
     1741 tgcaatacta aagggctccc ggtgaagcgg atcgttacca cggttatcat tttttcattt
     1801 attttttttc tgaacgggtt cgggcaggaa ggggttacct atgatctgaa aaaaccggcc
     1861 agatacgaaa accgcaccct gggatacgaa aaaacaacgc aaaccaaatt caagaccccc
     1921 cggcgcttcc tccagaatac cattacgcat tataattttt attacaatac caacaacaag
     1981 ctgaatgaag tggtcgcccg ggccaaagcc aactttcagg acaatttcac caaactcctc
     2041 cccttttaca actataacct ggaaatcact gcccacgata aacggaacct ggattcggtg
     2101 attgacaagg ttaataccgc aatcctcatt cgtgacctcc ggaacgattg ggcggataac
     2161 ctttatatgc tgatgggcca ggcttactat tacaaaaaca acctggactc ggcgtacgtg
     2221 ctttttcagt ttgtgaatta tgcttttgct ccaagagaaa aagacggcta ccccatcccg
     2281 atcggcagca atgccaatgc ggatgagggc gggaacgcgt ttatcatttc caccaatgaa
     2341 aaaagaaata tcgttaagcg cacattcagt cttccgccca gccgcaacga agcgttcatc
     2401 tggcagaccc gcactttcct gatgcagggc gaccttaccc gggcatccgt gctcatcgat
     2461 atgctgaagc aggacccgaa tttccccgaa cggctgcaga cttcactgca tgagctgcag
     2521 gccctcctgt attacaggca gtccaattac gatagtgccg cctatcatct ggatctggcg
     2581 ctggacaatg ccgttaccca ggccgaacgc gcccgctggg agtacctgat cggtcagttg
     2641 tacgaacgca gtaaccataa tatcgctgcc aaaagatatt ttgaaaaatc agcatcgcat
     2701 accctggatc cggtaatggg cgtatatgcc cggttgaacg ccatccggca gaacaagggc
     2761 aatgcggccg gagacgatta tatccgtaaa aacctggaag cgctgcatag aatgacccgc
     2821 aaggaattgt tcgcccctta ccgggatatc atttactatg ccgccggtga aatggagctg
     2881 gaaagaaata acaccgctgc ggcaaaagcg tattttaaaa aggcgattgc caattccggc
     2941 atcgagggca cgatgcgcga ccaggccttt ctgaagctgg gctggctata cctggatgaa
     3001 aaaaaatatc cggaggcaaa aagcgcctat gacagtgtga gtgctgcgga tcagctgatt
     3061 tccgattccg ttcagatcct gatggaccgc aaactggcgc tcgccaaagt agtacggcaa
     3121 atggtcatta ttgaaagcca ggatagcctg cagcgcattg ccgccatgac agaagctgaa
     3181 agaactgcct atattaaaaa aatgatccgc gcttaccgga aacaacgggg cctctccgaa
     3241 gagggatcat cggcggaaaa cggaaatcaa ttccggacaa atgccgcaaa tgccgatatg
     3301 ttcaatgata acaacagcgg cgaatggtac tttaacaatc cttccctgaa gtccaggggc
     3361 tacaatgatt tccactcgaa atggggcagc cgtccaaatg tcgataactg gcagctgcaa
     3421 tccctgatca ataaacaaaa accgggcgtt gctgccggca gattgaataa cgggaacggt
     3481 gcagaagcgg gcgcagctgc ccctgctccg gcagaactta cccccgaagg tcttttgcag
     3541 caggttccac tcacgccgga aaaattaaag agatcgcagg attcggtgga gaatgccctc
     3601 tatgccttgg gaaaagcact tcaggacctg atcccggatt accgttcagc catactcacg
     3661 tatgacagcc tggaaaaacg ttttccctcc acccgctatt accaggaagc cctgttcaac
     3721 acctattatt gctacgtgaa actgaacgac gcggagaatg ccgcccgcat actggccctg
     3781 atgaaacaaa aattccccac cggcaaatac gttgcgatta ttgaaaaccc gcacccggtt
     3841 gcgccggacc aggaaatcag gaacaatgcc acgcagaatt acgagaaaat atatcaggaa
     3901 ctgatcgaag ggcatttcga actggcgctc acggaaaaaa agaaacagga cagtatctac
     3961 ggcgacaggt actggacgcc gcagctgctc tacgtggaag ccctgtatta tatgcatacc
     4021 cggcacgaca gcctggccaa ggcaaccctc aaccacatca tttctacgta taccggaacg
     4081 ggcatggatg acaaagccaa aaataccctg cgcgttttaa atgaaaggga aaagatcgag
     4141 gaatacctga ccaaccttaa ggtgacccgg gccaaagacg acagcctcgg actggcagat
     4201 aacggcaaag accagacgaa gaccgccacg gctgataccg cagcggccaa gccggttgca
     4261 aaattgaaag tggacagtgc ccgggtatca aataataaac cggattccct tcaggctaag
     4321 aaacctgcga tatttgcctc tgcctttacc gctgctccgg ccagaccgca tgccgttgcg
     4381 ctgatcatgg ataaggtgga cccggtttac gtaacggaaa gcaaaaatgc atttgaccgg
     4441 tataaccggg agaatttcta tggcaaacat tttgacatca atatcgtggc attgagcgac
     4501 agtatcaaaa tcgtgctgat caacggattt gaaaatgcgg atgcggcact cgcctacctg
     4561 gataaaaccg ggaagctggc gggaagggaa attctgccct ggcttccggc aaataaatat
     4621 tcttttatta taattgacga tcagaacctg gccgtcctgc agacaaataa ggatatccag
     4681 gcttacaaaa agtttttatc cgtgtatttc cctggtaaat ttccgcccct gaaatagggt
     4741 cgtgaagaat accgggagat ccaatcgatt aataaaaaaa tatgcaaaag gcaaacccca
     4801 tattctggaa aatctttttt ggaggactag gcttattcat catcctcctg ctgctgatca
     4861 attttggcgc atttggaccg ctcccctcct taaaagaact cgaaaacccg tccatcatgc
     4921 tcgcgtccga agtgtatgcc gacgatggaa ctctgatggg aaaatattat aaggataaaa
     4981 gcaaccggag taatgtggaa taccgggaca tatccaaaga ggccatccat gccttgctgg
     5041 ctacagagga taaacgtttt tacgaacatt ccggaattga cgggatgggc gtattgcggg
     5101 cggtggttaa actgggccat gacggaggcg gaagcaccat cacccagcaa ctggccaaat
     5161 atatgctggg gcaaggatcg aaaaacatgt tacgccgcgg gatagaaaaa ttaaaggaat
     5221 ggatcacctc cgttcgcctg gaaagaaatt ttaccaagga agaaatcctg gccctgtacc
     5281 tgaacgccgt tccgttcggc gataatgtgt acggcatccg gaacgcttcc cgcaccttct
     5341 tccagaaaga accggaccga ctgacggtgg atgaagcggc catgctgatc ggcatgctca
     5401 gcggaaacac actatacaat ccccgaagaa atccgaaggc agccattgaa agacggaata
     5461 ccgtattaaa ccggatggtg gaaagcggct acctgggcga agccgaagca accaaactca
     5521 aacagaaacc cattgacctg tccaattata aaaagctgga cgagaataat ggcctggccc
     5581 cttatttccg ggacgtgatc cgcgaagatc tgaagaaatg gtgcaaggaa cataaaaatc
     5641 cggctaccgg cgaaccctac gatctgtacg aagacggatt gagaatttat accaccatta
     5701 atccgcggat gcagatctat gcagacgaag ccgttgccaa acagatgccc atgctgcaaa
     5761 aagcgctgag cgcacaggct tcgatccgga agggcctggt atggaaggat catcagaata
     5821 tcctggagtc cctgatccgg aacagtgagc gctggcaggc tatggaagaa gacggcattc
     5881 cggtggccga gatccggaag agctttgatc agccggtaca gatgaaagtg tttgcgtgga
     5941 atgtaaaaag ggaaaaagat accgtaatga cgccgctgga ttcaataaaa tataatcacc
     6001 agatgctgca gaccggcttc atggccatgg atccgatgag cggcgcggta aaagcctggg
     6061 tgggcggtat tgatttcaag acctataaat tcgatcatgt aaatatcaat accaaacggc
     6121 aggtgggttc ttccatcaaa cccctgctct attcggatgc cattgaagat gccggattta
     6181 cgccggaaac gccctgtgaa aatgaagcac agtattttga cggatacggg ctggtgccag
     6241 ccggcggaag atgcggaggg aacggcgccg tggtcagcat ggccactgcc ctggcttatt
     6301 cattgaattg cgcgtctgca tttattatga aacaggttac gcccaaacgg tttgtttcct
     6361 ttctgaaaaa tatcaatatt tcgtccaaac tggaacccta cccttccatt tgtctgggcg
     6421 ccgtggaaat ctccctatac gaaatgcttt ggggatatac catgtttccc tccggcggct
     6481 tcagcaccaa accctattat ttatcccgga ttgaagataa aaacgggaat gtgctggaca
     6541 ggtttgatac ggaaagaaaa gaagtcatca gccagaatac ggcctatacc atgtgccgca
     6601 tgatgcaggg tacggtagat ttcggaacag ccctgggtct gcgcgaccgt ctggggattt
     6661 cggaaatggg cggaaaaaca ggaaccacaa atgacaattc cgacgcctgg ttcatgggct
     6721 atacccccca gatcctggcg ggcgcctgga tcggttgcga cgaccggttc atccggctgg
     6781 aaaacgggct cgggcaggga gcagaagccg cccgtcccat ctgggagtat tttatggcca
     6841 aaaccttggc tgacaaagac cttggcctga gcagaaaggc caagttcgta caaccggaaa
     6901 atatgaagag cgatatgatg tatgactata tgaacatcat agataagacc ccgcccccgg
     6961 gagcggaagg agaaaaccag ggcaacggga atgccaacca gtacctggac accagctcac
     7021 ccagcatacc gattgattcc aaactttcca gcgacgaaca gaatgtactg aaagaagcca
     7081 atgctcctgc cagaaaaccc gatgcgtcaa aaacggaaaa tcctcccccg gaaaagaaaa
     7141 aaaagggatt tttcaggcgg ctatttgaca ggaaggacaa ataggtgaat caagggccgg
     7201 gaatttgagg cggaaagcat atatccagtg cacccggaaa atttttagcg ggagaggaac
     7261 cccttcccgg caaaggaaaa ataataaaaa aaggctcaaa aagagccttt attgttgcga
     7321 agggagggaa cggatcgtcc caatattcgc tgaaattctt tttgattgtc agtcatttat
     7381 aatttacgga cgcacaccgg acggcttaga aaaatcattt gttacacggt ttgttacaaa
     7441 aggtccttat ttttttttct tattagtgca aggtggtttg caatc
//
LOCUS       DASYCU010000008         5710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1597230, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000008 DASYCU010000000
VERSION     DASYCU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597230"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VGM24_00160"
     CDS             complement(<1..731)
                     /locus_tag="VGM24_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4153796.1"
                     /translation="MRLPKRLLWTASLVLAMTGVFAQSKNIRGRVTDSQTGNPLSSVT
                     VSLKNSTNRVATDPDGNFSIKPDGPGEMILECTIVGYATQQIKVSGNEFVRVSMQPEA
                     SGLNEVVVVGYGTQKRANLTGAVGTVKADALKDRSITNASQALAGQIPGLWVNQTSGQ
                     PGLDGATINIRGIGTLGNSTPLVLIDGIQGSFNGVNPNDIESISVLKDASSSAIYGSR
                     AANGVILIVTKSGKKGPLHVDLNSY"
     gene            908..1771
                     /locus_tag="VGM24_00165"
     CDS             908..1771
                     /locus_tag="VGM24_00165"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY4153797.1"
                     /translation="MKAQLIDISISGTKNIQIKKVDQFYLDTPFHFHKYCELVWIKES
                     FGKRVVGDKVDNFEEGDLVLMGPDLPHIWLNDPIFQLKKKGLRAKAIVVYFPHDFILN
                     LSDEPDVIQPTAGLLKKARRGLYFYGNTHKEVIRLLSKVEETGGFKKILTFLQVIEVL
                     SRSRDYTYLSSISYENLNDQKDTTRINKVYQFLMQNFHRDIKLQELASLSNMTPNAFC
                     RFFKSRTQKSFTRFLNELRIGHACKLLQDESYSVADVCYESGFNNPANFNKFFKSVTK
                     KTPSEYRKKSA"
     gene            complement(1781..2347)
                     /locus_tag="VGM24_00170"
     CDS             complement(1781..2347)
                     /locus_tag="VGM24_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEY4153798.1"
                     /translation="MEIIRSIQNRIYELRGERVMLDRDLAALYETETKALNLAVKRNI
                     KRFPKDFMFQLTREEFEGLRFQTESSNKREAALRLQNETSKGRGGTRYLPYAFTEQGV
                     AMLSGILNSDKAINMNIAIMRVFVEIRRFALQKTDLVVQLKEIQQRIGEHDIQLNGIY
                     DAIENLLDEKAAQTKWEDRERIGFKGKK"
     gene            complement(2497..4539)
                     /locus_tag="VGM24_00175"
     CDS             complement(2497..4539)
                     /locus_tag="VGM24_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153799.1"
                     /translation="MHWKRLILFFSFGSFITIPSLNAQVAYSGIQPGQAKAVAGNHRV
                     TLENQVLGLVFSDSNGRIAISSFQNKTTKEKLDFPETAPLFELRLKEGTRLSSADFVP
                     DGKIVTEDISGNPASGVFAGRLKGKRIRASFKDEKLRLHIEWEAELRDGSNYVRQVLR
                     FLPVDSVDFSRITLLSVPTSFGAHAVGVVDGSPMVKGTLFFAVEHPMSRIGEEGSALT
                     VFLSRSIPLTAGNPFTLSSVWGVTPENQLRRGFLYYLERERANPYHQFLHYNSWFDLS
                     YEDRVLNDSLCLDRIKVFADSLITRRKTPMDAFLFDDGWDDYRTIWQFNKGFPEGFSN
                     VRKAAEKYHAGIGVWMSPWGGYDIRKTQRLEYGARQNPPFEINENGFSLAGPVYFKWF
                     SQVVTDFIRKYHVSIFKFDGVGAGNGVSGVTGQYERDVESLIRLIGVIRKQKPDIFLS
                     LTIGTWPSVYWLKYGDVIWRAGDDTGLQGKGTKRQQWMNYRDGQTYRNVVERAPLYPL
                     NALMLHGLCIADHGLPGKLEMDNKQIADEIWSFFGTGTSLEEMYVNPHKLNTANWDCL
                     RDAIAWVRSKAGILPDVHWIGGDPAKEEVYGWAAWSPRGGVISIRNPSDQKKIFKINT
                     RAVFELPSGTPSRFNFYDARVKMNGKNKLVISNAASFDLELQPFEVKVLNALPEIK"
     gene            complement(4597..5628)
                     /locus_tag="VGM24_00180"
     CDS             complement(4597..5628)
                     /locus_tag="VGM24_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4153800.1"
                     /translation="MGTDKKKKKSGSAAPGRITVGVVGLGLMGCSISTCLLMAGHRVI
                     AVAPLPADLEHAESRIREHLLKSQKEGMVRKRPDHYMKNLELTENYQSLKPCGLVIEC
                     TLENLEIKKAVYNRIESVISSDSILTSNTSAIPISLLQVQTTHPERFLGLHWAEPSHT
                     TRFLEIICGDRSDVKKGEYLYDLAHAWGKEPTLVRKDIRGFITNRLMYAMYREAFNLV
                     ENGYASVEDVDRACRNNAGYWMTLVGVFRWMDLTGVAAYHTVMKDLFPTLNNSTAVPG
                     LIDGIVKAGGRGVSNAKGFYNYTKEEARLWEETFKDFSYEIRKLALKYPADVVKKKLG
                     KKRTGKKIL"
BASE COUNT         1427 a         1506 c         1238 g         1539 t
ORIGIN      
        1 cagtaggaat tgaggtcaac atgcaaaggg ccctttttcc cgctcttggt tacaatcagt
       61 attacaccgt tcgctgccct ggatccgtag atggcagaag atgaagcatc tttcaaaaca
      121 gaaatggatt caatatcatt cgggtttact ccattgaacg acccctgtat tccatcgatc
      181 aaaacaaggg gagtactatt tcccagtgtt ccgattcccc ggatatttat cgtcgcgccg
      241 tccaggccgg gttgccctga agtctgattc acccaaaggc cggggatctg acccgccagg
      301 gcttgtgatg cattcgtaat agaccggtct tttaatgcat ctgctttcac ggtacccaca
      361 gcgccggtca gattggccct tttctgggtc ccgtaaccca ctacgaccac ttcattcagt
      421 ccgctggctt cgggttgcat ggacacccgt acaaattcat tcccgctgac ttttatttgt
      481 tgtgtggcat aaccaacgat tgtacattcc aggatcattt cccccggccc gtccggctta
      541 atgctgaaat tgccatccgg atcagtggct acccggttgg tcgaattctt caggcttacg
      601 gtgacggaag ataacgggtt accggtctgg gaatctgtta cccgtcctct gatatttttg
      661 ctctgcgcaa aaacgccggt catagccagc acgaggctgg ctgtccatag caatcgtttt
      721 ggcaatctca taatagcatc atttttgaat tgctaaatta gcgaggaaag acagagcaat
      781 cctacaagcc gcttacctaa tatgtaaact attctaccaa ttttacatcc cgtttggcaa
      841 aatctggtta atttcatttc taaatgatcc tccggctgtc aaaagatcaa atttattttt
      901 aaacatcatg aaagcacaac tgattgacat atcgatatcc ggaaccaaga acatccagat
      961 aaaaaaagtg gatcaattct atctggacac gccctttcat tttcataaat actgcgaact
     1021 tgtctggata aaagaaagtt ttggcaaaag agtggtcggg gacaaggtgg ataattttga
     1081 agaaggcgac ctggtcctga tgggcccgga cctcccgcat atctggctaa acgacccgat
     1141 ttttcagctg aagaaaaaag gactgcgcgc aaaagcgatc gtcgtttatt tcccgcatga
     1201 ctttatcctt aacctgtctg acgaaccgga cgtcatccag cctactgccg gcctgttgaa
     1261 aaaagcgcgc cgcggccttt atttttatgg caatacccat aaggaagtca ttcgtttatt
     1321 gtcgaaggtg gaagaaacgg gcggttttaa aaaaatcctc acctttctcc aggtaattga
     1381 agtgttgtcc cggtccaggg actacaccta tctctccagc atttcctacg aaaacctgaa
     1441 tgatcagaaa gataccaccc ggatcaataa ggtataccag tttctgatgc aaaatttcca
     1501 tcgcgatatc aagctgcagg agctggccag cctgagcaat atgacgccca atgccttttg
     1561 ccgctttttc aaaagccgca cccagaaatc gtttacccgt tttctgaacg aattgcgcat
     1621 tggtcatgcc tgcaagcttt tgcaggatga aagttattcc gtggcggacg tttgttatga
     1681 aagcgggttc aacaatcccg ccaatttcaa caaattcttt aagagcgtta caaaaaaaac
     1741 accttccgag tacagaaaga aatctgcgta aatggttttc ctattttttt cctttgaaac
     1801 cgatccgctc ccggtcctcc cattttgttt gggcggcttt ttcgtcgagc aggttttcaa
     1861 tggcatcata aatcccgttc agctgaatat cgtgttcgcc tatccgctgt tgtatttcct
     1921 ttaattgtac gaccagatcc gttttctgca aggcgaacct tcttatttcc acgaaaaccc
     1981 gcatgatggc aatattcata ttgatggcct tgtcggaatt caggatcccg cttagcatgg
     2041 caacgccctg ctcggtgaac gcatagggca gataccgggt accgcccctt ccctttgagg
     2101 tttcattttg caacctcaaa gcagcttccc ttttgtttga ggattcagtt tgaaacctca
     2161 acccttcgaa ttcctcccgg gtaagctgga acataaaatc cttcggaaaa cgtttgatgt
     2221 ttcgttttac ggccagattc agcgcctttg tctccgtttc atataaggcc gccagatccc
     2281 ggtcgagcat tacccgttca cccctcaatt catagatcct gttttgaatg ctgcggatga
     2341 tctccatatg gatgatttta aaattttaaa agcagcctga acggcccttt aaagaaaagg
     2401 gattccgtca ttaattacaa gtagatgaaa acgcagggca aagaatgagc caatatttcc
     2461 ggcaattccc gggccgggaa tcccgggcct taaaaatcat ttgatttcag gaagggcatt
     2521 caacactttt acttcaaagg gctgaagctc cagatcaaag gatgctgcat tggaaatgac
     2581 cagcttgttt ttcccattca tcttaacccg ggcatcgtaa aaattaaacc gggaaggtgt
     2641 tccggagggc agctcaaaaa ccgccctggt atttatctta aaaatttttt tctgatccga
     2701 tggattccgg atgctgatca ctcctcccct gggagaccag gccgcccagc catatacttc
     2761 ttcttttgcc ggatcgcctc ctatccagtg aacatccggc aatatcccgg cctttgacct
     2821 gacccaggcg atcgcatccc gcaaacaatc ccagttcgcc gtattcagtt tatggggatt
     2881 cacatacatt tcctccaggc tggtgccggt accaaagaaa gaccagatct cgtctgcgat
     2941 ctgtttatta tccatttcca gtttccccgg aagtccgtga tccgcaatac acagcccgtg
     3001 aagcatgagg gcatttaacg gatacagggg cgcgcgttcc accacattgc ggtacgtttg
     3061 cccatcccgg taattcatcc attgctgacg ctttgttcct ttaccctgga gaccggtatc
     3121 atctccggca cgccagatca cgtctccata tttcaaccag taaacggaag gccaggtgcc
     3181 aatggtaaga cttaggaaga tgtccggttt ctgtttccgg atgactccga tcagccggat
     3241 cagcgattca acgtctcttt cgtattgacc cgttaccccg ctcactccgt tccccgcccc
     3301 cacgccatca aacttgaaaa tcgaaacatg gtatttccgg ataaaatcag tgaccacctg
     3361 gctgaaccat ttaaaataaa ccgggccggc cagcgaaaaa ccattttcat ttatctcaaa
     3421 aggaggattt tgccgtgcgc catactccag ccgctgggtt ttccggatat cgtatccacc
     3481 ccagggcgac atccacacac caatgccggc atgatatttt tccgccgctt tccggacatt
     3541 gctgaatcct tccggaaatc ctttattgaa ttgccagatc gttctgtaat catcccagcc
     3601 gtcatcgaat aaaaacgcat ccatcggcgt cttgcgcctg gtaatgagac tgtcggcaaa
     3661 tactttaatc cggtccagac aaaggctgtc gttcaatacc cggtcttcat aagacaaatc
     3721 aaaccaggaa ttataatgga ggaactgatg ataaggattc gccctttccc tttccaggta
     3781 atacagaaat ccccttctca attgattttc gggagtaact ccccaaacgg acgagagggt
     3841 gaagggattc ccggctgtca ggggtatgga cctggaaaga aagaccgtta aggcggatcc
     3901 ctcttccccg atccggctca tgggatgttc cactgcaaaa aacagagttc cctttaccat
     3961 cggggaaccg tccaccacgc caacggcatg agctccaaaa gaagttggga ccgagaggag
     4021 tgtgatccgg gaaaaatcaa ccgaatccac cggtaaaaac cggaggacct ggcgcacata
     4081 gttggacccg tcccttaact ccgcttccca ctcaatatgc aatctcagct tttcatcttt
     4141 aaaagaagcc cgtatccttt tgccctttaa cctgccggca aaaaccccac tggcggggtt
     4201 tccggaaata tcctccgtaa cgattttccc atccgggaca aaatcggcgg agctgagccg
     4261 ggttccctct ttcaaacgga gttcgaaaag aggcgccgtt tccggaaaat ccagtttttc
     4321 ttttgtcgtt ttgttctgaa aggaagaaat ggctattctg ccatttgaat cggaaaaaac
     4381 aagaccaagc acctggtttt ccagagtcac ccggtgattg cccgcgacgg cttttgcctg
     4441 tccgggttgt attccactat aggcaacctg cgcatttaaa gaaggaatcg taataaatga
     4501 tccaaaactg aaaaataaaa tcaaccgttt ccaatgcatg tctgaatatt tttatttagg
     4561 caatccccaa ccgggttccc gtttcttgtg atctccttat aaaatctttt tgcctgttct
     4621 tttctttccc agctttttct taaccacatc ggccggatat ttcagggcaa gcttccggat
     4681 ctcataagaa aaatccttaa acgtctcttc ccacaaacgg gcttcttctt ttgtgtaatt
     4741 gtaaaatcct ttggcgttgg aaaccccccg ccctcctgcc tttactatac catcaataag
     4801 cccgggaacg gctgtcgaat tattcagggt gggaaaaaga tccttcatta ccgtatgata
     4861 ggcagctacg ccggtcaggt ccatccagcg aaaaacgccc acgagggtca tccagtatcc
     4921 cgcattgttc ctgcaggccc ggtctacatc ctccacgctt gcataaccgt tttccaccag
     4981 attaaaggcc tcccggtaca tcgcatacat cagccggttg gtaatgaaac cccggatatc
     5041 ctttctgacc agggtgggct ccttgcccca ggcatgagcc agatcatata agtattcgcc
     5101 ctttttgaca tcgctccggt caccgcatat gatttcaagg aaccgggtgg tgtgagaggg
     5161 ctcggcccag tgtagtccca aaaacctttc cggatgtgtt gtctgtacct gaagcaggct
     5221 gatgggaatg gccgatgtgt tgctggtaag aatcgaatca gatgaaatga ccgattcgat
     5281 ccggttatag acagcctttt ttatttccag gttctccagg gtgcattcga tgaccaggcc
     5341 gcagggtttc agggactggt aattttccgt cagttccagg tttttcatgt aatgatccgg
     5401 gcgtttccgg accatccctt ctttttggga ttttagcaga tgctcccgga tcctgctttc
     5461 cgcatgttcg aggtcggccg gcaagggggc aacggcaata acccggtgac ccgccatgag
     5521 caaacaggtg ctgatgctgc atcccatcaa ccccaggccg accaccccta cggttattct
     5581 tccgggcgct gcactaccgg attttttttt cttcttatct gtacccataa atccgtttta
     5641 tttttttgac tcaataggtt gcccttccgc ccgaaagatc ataggtgaac ccggtagtaa
     5701 agctgttttc
//
LOCUS       DASYCU010000009        13773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2466462, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000009 DASYCU010000000
VERSION     DASYCU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466462"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VGM24_00185"
     CDS             <1..519
                     /locus_tag="VGM24_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HEY4153801.1"
                     /translation="EHAKARGARIYCEIAGCGATADAHHITAPHPEGLGAKNVMNAAL
                     RDAEMTASDIDYINTHGTSTPLGDISEIKAIMDVFGEHAFKVNISSTKSMTGHCLGAA
                     GVVEAIACIMSVVNDVVPPTINHFTDDPDLDPRLNFTFLKPQKRTVNAALSNTFGFGG
                     HNACVIVKKFEG"
     gene            592..1326
                     /gene="rnc"
                     /locus_tag="VGM24_00190"
     CDS             592..1326
                     /gene="rnc"
                     /locus_tag="VGM24_00190"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY4153802.1"
                     /translation="MGLLDWILKGDSSIVSFRKQLRNVLGFVPGNAVLYKTALTHRSV
                     KESSDQNNERLEYLGDAILSAVIADFLFKKYPYKEEGFLTEMRSKMVNRVQLNDIAVK
                     MGLRKISYFNKFDGSLKMSQIFGNTLEAVVGAIYLDKGYDKTRQWIMERIIQPYLFMD
                     DLENLEINHKNKLYGWANKNGKNLEFETLDEHMEGGRRLFTVGAVVNGELIAQGRAYN
                     KKDASQIAAQQAMEKLGLNKIEGEDN"
     gene            complement(1371..1775)
                     /gene="mce"
                     /locus_tag="VGM24_00195"
     CDS             complement(1371..1775)
                     /gene="mce"
                     /locus_tag="VGM24_00195"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY4153803.1"
                     /translation="MQKIEHIGIAVKSLADSIPLFEKLLNRTCYKTEMVESESVKTAF
                     FALGDHKLELLESVDPEGVIARYIQRQGEGIHHIAIAVEDIEAEMKRLVAEGFRLLKD
                     KPVPGADNKLVCFLHPKGTNGVLIELVADRAR"
     gene            1870..3033
                     /locus_tag="VGM24_00200"
     CDS             1870..3033
                     /locus_tag="VGM24_00200"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEY4153804.1"
                     /translation="MLKLPVYLDHNASTACDPRVLEAMLPYFTKKFGNAASRTHSFGW
                     EAEEAVKFARERVARLINAIPEEIVFTSGATEADNLAVKGVFDMYSAKGNHIITVVTE
                     HKAVLDTCKHLEKSGADITWLSVRSDGLLDAAGLEAAIKPNTILIAVMYANNEIGVLQ
                     PVRTIAEIARKHGVLFFTDASQAVGKIPVDVIRDGIDLMAFSAHKIYGPKGTGALYVR
                     RKNPRVRLTAQMDGGGHERGMRSGTLNVPGIVGFGEACDICLREMDQESARLRILRDK
                     LENALLQIEETVVNGNRESRLPQLSNISFRYVDENALMMGLNKEIALSSGSACTSANP
                     EPSYVLKALGLDDDLAHSSLRFGLGRETTEEEIDFTIKRVTETVGKLRLFSYL"
     gene            3045..3377
                     /locus_tag="VGM24_00205"
     CDS             3045..3377
                     /locus_tag="VGM24_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY4153805.1"
                     /translation="MIYVSEKAKEEVRRLMEEAAIADKPEYFLRVSVVSGGCSGLSYK
                     MDFDKEKKPMDQEFEDNGVKVVTDLKSFLYLVNTTLDFSEGLNGKGFFFNNPNASRSC
                     ACGESFAV"
     gene            3602..5911
                     /locus_tag="VGM24_00210"
     CDS             3602..5911
                     /locus_tag="VGM24_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4153806.1"
                     /translation="MKTYFRRMTNTRVLIVLMLFASCANTAQAQIPSFDFGKSYVNIS
                     KGLNGGTIEPGDILEIRATFVVGNTGYADSCGYFDAIPANTSFVPGSLAILTNEGKLY
                     KSFTDAPGDDEGYISGVNIRINIGYKTNPRATAFRRGRVSFNDKPSFYGSTCILIASF
                     RVVVTGTYGNSISLGGGSITYRPNGTGSVKSNVFSPDNVMFFKNLGICTNTSGTNAIL
                     SEFGGTFGSGKAKNRAASGKVSNNYIDTTFGPNSPQDYYYGISNNTSTGGVGYTTLNT
                     WPIPDVSIPPTSQTHRVFGIWDIIGDHTGAVNPLLGNPATDTVTGTGGYMAVINSSYR
                     TGIAFLDTVNNLCPNTYYQYYAWFRNICSRCACDSNGKPPGTSGYIPTGVNDSSGVHP
                     NLTFNVNGFDYYTSGNILYTGQWIRKGFTYLTGPAQTSMIIYIRNNAPGGGGNDWAID
                     DIGVATCTPQISLTPDKPDTLCRGADDTVRFKISAFFNNYTEWKIEKSVDGGSTWASP
                     GLDTLGNAASGSSVPVYNALSGLYEYTVTRYYRLNTADPIVIYRLVLASTPGNLNDAN
                     CRFITSSPKYVRTVDCNVVLPTELRLKGTVSSGYAQLQWISSQETGNILYSIERSDDE
                     GAHYITAGTASGTATAGNGASYSFQDPKSLTGAALYRIRINDQRYQNYSNTILLSSRD
                     IPLAISGLPNPFYSSVSFNLTAPEDQDITLSLYDAYGRLLTRESRTAYKGLNHFDLSR
                     TGTLQSGMYVLQVLYKDQMITRQLIKKLE"
     gene            6166..6888
                     /locus_tag="VGM24_00215"
     CDS             6166..6888
                     /locus_tag="VGM24_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4153807.1"
                     /translation="MWAVCKKEFRQFFSSLTGYIAIVVFLLVTGLLLFVFPDTSILDF
                     GYASLEKFFDLAPWVLLLLIPAISMRSFSEEFRSGTFEILQSKPLTQWQIVNGKYLAA
                     LLVIGIALIPTWIYYFSVQQLAAGTGMDTGAAFGAYIGLFLLTAVFAAIALCCSSFTT
                     NSIIAFIASGFSCFLIYTGFHAISRISSLPAGFDYYIDMLGIDFHYRNMSRGLITMRD
                     AVYFISVILFFLILTKNNLNKR"
     gene            6897..8609
                     /gene="gldG"
                     /locus_tag="VGM24_00220"
     CDS             6897..8609
                     /gene="gldG"
                     /locus_tag="VGM24_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HEY4153808.1"
                     /translation="MNKIFSFNSGWLFPLLLLILINAGASFWSLRFDLTSEKRYTLSA
                     PVKKMLNNIHDQVNIDIYLQGNLKSGLRKLRNSTEALLQEFNDYADGNIHYRMVDPVA
                     GGDDSTRSMILDSLGRMGIQPMTLVAQAKKGEEQTQRIVIPAAMVKYKNRIFPVDLLK
                     GVQRSREGQPPEQLYVNAETLLEYKFANAIDKLLKDTLPAVGYLMGNGEPLDFRVYSL
                     IQYLRENYRFGMLRLDSLPVIPSRVNALIMVKPSSKFTDDEKRKLDQFVVHGGSLICM
                     IDNLHAEPDSLQVSKEVIAYDRGLDLDDLLFHFGVRINQDLVEDMQNASINLVVGSQG
                     GKPQLQLEPWPYYPLLDGNPSSPISKNLDPVFSRFANSIDTVKAPGIRKSILLQSSPN
                     ARTISTPARITLESIKTATDPKAFNRQNIPVAVLLEGKFHSLYANRISPEMEDSLDKF
                     YHQPFLRTGEKTARVIVCADADVLMNELSDRGPLPLGFSKDINYTFANMDFLQNCLDY
                     CVSSSGIMEARSKDYTLRLLDPEKTEDDRTFWQFVNIVCPLLVILLGAVIFHFIRKRK
                     YSIR"
     gene            8641..9570
                     /locus_tag="VGM24_00225"
     CDS             8641..9570
                     /locus_tag="VGM24_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEY4153809.1"
                     /translation="MTHSQFAYLIFGIVLVLALVFDLGLLSKKGAVITIKKALLQTAF
                     WVILAMAFFLFLWVDENRSTALEYLSAYLMEWSLSIDNIFVFILIFSFFGVKEKFFSR
                     VLLIGILLAVFFRIIFITAGIVLVERFGWLLYIFGAILLYTGLTMFFAKKDKGTNLEE
                     NKVYRFLKRFLPLTSEDGGGKLRTRINGKKFYTSIFVVIVLLATTDIVFALDSIPAVF
                     GITQNRLVIYTSNIFAVLGLRSLFFLLRGAVDKFRFLQQGIATVLVFIGLKMLSELIH
                     IKVSVLISLVVILVCILTSIVVSLVWDKKHPSS"
     gene            9585..12062
                     /locus_tag="VGM24_00230"
     CDS             9585..12062
                     /locus_tag="VGM24_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HEY4153810.1"
                     /translation="MGYSIRHIASVINGNFLQFRQDDPVEYLLTDSRRLLFPETSLFF
                     ALYGPRRDAHSFLPDLYKRGLRNFIVYRKVEIKDFPDANIVLVGDSLKALQMLAAFHR
                     RRFDYPVIGITGSNGKTIVKEWLNELLEDKYQIVRSPRSYNSQIGVPLSVWLMNETHT
                     LALLEAGISKTGEMKALEEIIHPGIGVFTNIGGAHDEGFENTEAKIREKLLLFEASDR
                     LIYPSDDKVLHQTILNWQQSHSAVKLFSFGKKGSGLQVIAQKKNPDSTELTLSYEGKT
                     WPLLLGFVHDAAIQNTLTCITVLLYLGISPEDLSSRLLRLSPVAMRMEIQTGINHCSV
                     INDSYSADISSLKMALDFLSRQQQHPKRTVILSDILESGRKPEGLYQEVAVLLKKFKV
                     DRLIAIGGQMLANRNLFAGSGVKEQEFHPSTDLFLSSFQPHAFREETILLKGARVSGF
                     ERIDQLLSKQVHETLLEVNLEAMAHNLKQYQQLLEPSTRVMAMVKAFSYGSGGFEIAR
                     LLEFHKVDYLAVAYADEGISLRNSGIRLPIIVMNTEESAMDALLEYQLEPVLYSFSIL
                     RAMDKRLKREAIGLFPVHIELETGMNRLGFSAKEIPELLESLAHASFHVKSVFSHLAA
                     SEAADQDGFTQKQFQRYQSMLGQLQPGLPRPFLKHISNSAAVVRNPEFQLDMVRLGIG
                     LYGIDPSASKRLELKTVGTLKTTIAQIKSVAAGETIGYNRKGVADHPMQIATVRLGYA
                     DGYPRSLGNGTGKMWYKGRLVPTIGNLCMDMTMLDITGIENAREGDELIVFGPELPVE
                     QVAGWAGTIPYELLAGISQRVKRVYFE"
     gene            12127..12879
                     /locus_tag="VGM24_00235"
     CDS             12127..12879
                     /locus_tag="VGM24_00235"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEY4153811.1"
                     /translation="MNIKERKVLFIVLLIIVADQVLKIWVKTHMPLSHNWAAYYRLPV
                     SPYENGIQVLGKKFQLFFVENEGMAWGWKFGGNLGKMALTLFRLAAVIFGVYYIRLLI
                     KRNYHNGYIICAGLIFAGALGNLIDSMFYGLIFQESTYDHVAKIFPSHGYAGFLHGRV
                     VDMLYFPLIKTHYPHWFPFVGGEEFEFFSPVFNIADASISIGVIVLFLFQKRFVKKQA
                     EPETQDADHLRLKTEEETRNPEPLTPNPEQGL"
     gene            13042..>13773
                     /locus_tag="VGM24_00240"
     CDS             13042..>13773
                     /locus_tag="VGM24_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4153812.1"
                     /translation="MKKFLPVLSCLLLSFIGYGQIVSFNGEYFKIDTLYPRYDIPSHN
                     KGLSFPWEITYGPDDSLWVTEAHRYRIWKIHPGNKGNRILLDLEGLTTFNYYDPPNGY
                     PAPQGGLMGLAIHPLLLSGKPYVYVAMVYDVYPTSGSPNNNSCGGSGSVTNNPCFYKT
                     KIIRYTYNLVSHSLGSPVIVLDDLMGSNDHNSGRLKVSPVPESDGKYHLYYTIGDQGA
                     GQFNNGFRTNNAQNKDVYEGKVLRLN"
BASE COUNT         3440 a         3391 c         3433 g         3509 t
ORIGIN      
        1 gaacatgcaa aagcacgggg agccagaatc tactgtgaga tcgccggttg cggtgcaacg
       61 gccgatgccc atcatataac cgccccgcat cccgaaggac tcggtgccaa aaatgtaatg
      121 aacgccgctt tgcgcgatgc agaaatgacg gcttctgaca tcgactatat caatacccac
      181 ggcacttcta ccccgctggg tgatatttct gaaataaaag ccattatgga tgtatttggt
      241 gaacatgcgt ttaaggtcaa tatcagttcc accaaatcca tgaccgggca ttgcctcgga
      301 gcggccggtg tggtcgaagc cattgcctgc attatgagtg tggtaaacga tgtcgtaccg
      361 cccaccatta accatttcac cgacgatccg gacctggacc cccgcctcaa tttcaccttc
      421 cttaaacccc agaaaagaac cgtaaatgcg gcactgagca acaccttcgg gttcggcggg
      481 cacaatgcct gcgtgattgt gaagaaattt gaaggctagg cgaaagcaag ccggcccgcc
      541 ccttccacat tctctccttg ttttcacctt agaataacta tttttgatcc cgtgggatta
      601 ttagattgga tattaaaggg ggattcgtct attgtttcat tccgaaaaca actgcgcaat
      661 gtgctgggtt ttgtaccggg caacgcggtc ttatataaaa cggcccttac ccaccgctcc
      721 gttaaagaaa gctccgacca gaacaatgaa cggctcgaat acctgggaga tgcaatcctc
      781 agtgccgtca ttgccgattt tctttttaag aaatacccgt ataaagaaga aggatttctc
      841 acggagatgc ggagtaaaat ggttaaccgc gtgcaactga atgatatcgc cgtgaaaatg
      901 ggtcttcgaa agatcagtta cttcaataaa ttcgacgggt ccttaaagat gagccagatc
      961 tttggaaata cgctggaagc cgtggtgggc gccatatacc tggataaagg atacgacaaa
     1021 acccgccagt ggatcatgga acgcatcata cagccctacc ttttcatgga tgacctggaa
     1081 aatctggaga tcaatcataa gaacaaactc tatggctggg caaataaaaa cgggaaaaac
     1141 ctggaatttg aaacccttga cgaacatatg gaaggtggtc gccgcctctt taccgtgggc
     1201 gccgtggtaa acggcgaact gatcgcgcag ggacgtgcgt acaataaaaa agatgccagt
     1261 cagatcgccg cccagcaggc tatggagaag ctcgggctga ataaaattga aggggaagac
     1321 aattgaaaga cgggaagcca gcggcaggct taaaattaaa cactccccat ctatctggcc
     1381 cggtcggcaa ccaactcaat aagcactccg ttcgtccctt tcgggtgaag aaagcaaacc
     1441 agcttattgt ccgctcctgg caccggctta tccttcaata accgaaatcc ttcggcaacc
     1501 agtcttttca tttcggcctc gatatcctcc acggcgatgg cgatatggtg tattccttcg
     1561 ccctgccgtt gaatatatct ggcgatcacc ccctccgggt ccacactctc cagtaattcc
     1621 agcttatggt cgcccagggc aaaaaaggcg gtcttcaccg actctgattc aaccatttcc
     1681 gttttgtaac aggtccggtt caacaacttc tcaaacagcg gaatggaatc ggccaggctt
     1741 ttaaccgcaa tcccgatatg ttcgatcttc tgcatgagca aagatggggg aaaaagcccg
     1801 atgcccgccc accccccggg atcaactttt tccatcgtac ctttacgggt tcaataagaa
     1861 tattcggcca tgctgaaact gccggtttac ctggatcaca atgccagcac agcctgcgac
     1921 ccgagggtgt tggaggctat gctgccctat ttcacaaaaa agtttgggaa tgcggcaagc
     1981 cgcacccact cttttggctg ggaggcggag gaggcggtaa aatttgcgcg ggagcgggtg
     2041 gcccggctga tcaatgccat tccggaagaa atcgttttta cttccggcgc aacggaggcg
     2101 gacaacctcg cggtaaaggg ggtatttgat atgtattcgg ctaagggaaa ccatatcatt
     2161 accgtggtta cggaacataa ggcggttttg gacacctgta agcatcttga gaaatcaggg
     2221 gcagatatta cctggctttc cgttagatcc gacggcctcc tcgatgctgc cggactggag
     2281 gcggccatca aacccaatac gatcctgatt gccgttatgt atgcgaacaa cgagatcggt
     2341 gttttgcaac cggtaagaac tattgcggag atcgccagga aacacggcgt tttatttttt
     2401 accgatgcca gccaggcggt cgggaagatt ccggttgatg tgatccggga cggcattgac
     2461 ctgatggctt tctccgcaca taaaatttac ggacccaagg gaacgggcgc tttgtatgtc
     2521 cgccggaaaa atccccgggt tcgcttaacc gcccagatgg acggcggcgg acacgagcgg
     2581 ggaatgcgga gcggcacact gaacgttccg ggtatcgtgg gctttggaga agcctgtgat
     2641 atttgtttga gggaaatgga tcaggaaagt gcccggctaa ggatcctgcg cgataaactt
     2701 gaaaatgctt tgcttcagat tgaggaaacg gtggtgaacg gcaataggga atccaggctg
     2761 ccccagctca gcaatatttc tttccggtat gtggatgaaa atgcgctgat gatgggtcta
     2821 aataaagaaa ttgccttatc ctccggatct gcctgcacct cagcgaaccc ggagcccagc
     2881 tatgtgttaa aagcattggg cctggacgat gatctggcgc acagttccct gcggtttgga
     2941 ttgggaaggg agacgacaga agaagaaatc gactttacga taaagcgggt gacggagacg
     3001 gttggcaaac tcagattatt cagttattta taattaattc catcatgata tacgtatctg
     3061 aaaaagcgaa ggaagaagtc agacggctca tggaagaggc cgccatcgcc gacaagccgg
     3121 aatatttttt aagggtatcg gtggtcagcg gcggctgctc cggattgagc tataaaatgg
     3181 actttgacaa ggagaaaaaa ccgatggacc aggaatttga ggataatggg gtgaaagtgg
     3241 tcaccgatct gaaaagcttc ctgtacctcg ttaatacgac cctcgatttc tctgaaggat
     3301 tgaacgggaa aggctttttc ttcaacaacc ccaacgcaag caggtcctgc gcttgcgggg
     3361 agagttttgc ggtttagggc ggcgacttga taaacagttg atattcagtc atattttcgt
     3421 aaaacccggc ctgatggcta tatccggacc aggccgccaa atattttcga cgactggccg
     3481 cttttcttct ttttttatcc ccaaaaggta gatttacgct ttgaaatagg agggagagtc
     3541 actactttta agccgactaa acccattctg gtttattgat aacccagaaa agtccgatac
     3601 catgaaaacc tatttccgac gtatgaccaa tacgcgggtc ctaattgtcc tgatgctttt
     3661 tgcatcttgt gccaatacag ctcaggccca gattcccagt tttgacttcg ggaagagtta
     3721 tgtcaatatc agcaaaggat taaatggagg aactatcgaa ccgggtgata tcctggagat
     3781 ccgggccact ttcgtggtcg gaaacaccgg gtatgcagac agctgcggat atttcgacgc
     3841 cataccggcc aatacgagtt ttgttcccgg atcgctggcc attctgacaa atgaagggaa
     3901 actgtataaa agttttacag atgcaccggg cgatgatgag ggctatattt ccggtgtcaa
     3961 tatccggata aacataggtt ataaaacgaa tccgcgggca actgcttttc gccgcggccg
     4021 cgtctccttc aacgacaaac cctcttttta cggaagcacc tgtatattga ttgcgagttt
     4081 tcgcgtggtg gttaccggca cctacggcaa ttcgatcagt ctgggcggcg gatccatcac
     4141 gtaccggcct aatggtaccg gcagtgtaaa gtcgaatgtc ttttctccgg acaacgtaat
     4201 gttttttaag aatctcggaa tttgcaccaa cacttccgga accaatgcca tcctttctga
     4261 attcggagga acgttcggtt ccggcaaagc aaagaacagg gcggcctcgg gaaaggtttc
     4321 caacaattat atcgatacga cgtttggccc taattcgccg caggattact attacggcat
     4381 ttccaacaat accagcaccg gcggagtcgg atatactacc ctgaatacct ggcccatccc
     4441 ggacgtgagc ataccgccca cttcccagac gcaccgtgta ttcggaatct gggatattat
     4501 cggcgaccat accggcgcag tgaatccttt gctgggcaat ccggcaaccg atacggtaac
     4561 cggtacgggc ggatatatgg ccgtcatcaa ctcttcttac agaacaggga tcgcctttct
     4621 ggatacggta aataatcttt gtccgaatac ctactatcag tattacgcct ggttccgcaa
     4681 tatctgttcc cgatgtgcct gtgactccaa tggaaagccg ccgggcactt ccggttatat
     4741 tcctaccggc gtgaacgact cttccggtgt gcatcccaac ctcactttta atgtgaacgg
     4801 attcgattat tataccagcg gaaatatttt atataccggc cagtggataa gaaagggatt
     4861 tacctatctg accggtccgg cgcaaacgag catgatcatt tatatccgca acaacgcccc
     4921 ggggggcggc gggaatgact gggcaattga tgatatcggg gtggcgacct gtacacccca
     4981 aatctcgctg acgccggata agcccgacac gctgtgccgg ggcgcggatg atacggtaag
     5041 atttaaaata tcggccttct ttaataatta tacagaatgg aaaattgaaa aaagtgtgga
     5101 tggcggctct acctgggctt ctccgggtct ggataccctg ggtaatgcgg cttccggctc
     5161 ctctgttccg gtttataatg ccttaagcgg attgtatgaa tacaccgtaa cgcggtatta
     5221 ccggttgaac accgcggatc cgatcgtcat ttaccggctg gtgctggctt ccacgcccgg
     5281 caatctgaat gacgccaatt gccggttcat cacgtcctcc ccgaaatatg tgcgtaccgt
     5341 tgactgtaat gtggtgcttc cgaccgagct ccggttaaaa ggaacggtca gcagcggcta
     5401 tgcccagctc cagtggatca gcagccagga aacgggaaac attctttaca gcattgaaag
     5461 aagtgatgat gagggggccc attacataac cgcgggaacg gcttccggaa cagctaccgc
     5521 cggcaacggg gcaagctatt ccttccagga tccgaaatcc ctgaccggag ctgctcttta
     5581 ccgtatccgg atcaatgacc agcgatatca gaattatagt aatacgatcc tgctgagcag
     5641 ccgggatatt cctttagcca tcagcggcct gccgaatcct ttttatagtt cggtctcgtt
     5701 caacctgacc gctccggaag atcaggatat taccctgagc ttatatgatg cgtatggaag
     5761 gctactgacc agggagtccc ggacggcata caaaggattg aaccattttg acctcagccg
     5821 gacgggtacc ctgcaaagcg gaatgtatgt attgcaggtg ctgtacaagg atcagatgat
     5881 cacgcggcag ctgataaaaa aactggaata gcatccggct ggccagtggc caaaaaaaga
     5941 tcccgccgga gcgggatttt taagcaagta gcaatggtaa tcaattttca attaccggtt
     6001 attgttaaat gcgttggtgg gtggcccgca tctgtattcc ggcgtttttc aaaagcggga
     6061 cgaatatata agaaaatgat ttgttaaaaa aaattatttc catccccttc ctaggatttt
     6121 tgaacccccc gcgcagcaag ccattatatc tgttattttc gtgctatgtg ggcggtttgc
     6181 aaaaaagaat tccggcaatt tttcagcagc ttgacaggct atattgccat cgttgttttt
     6241 ttactggtta ccggcctgct gctttttgtt tttcccgata ccagtattct ggattttggg
     6301 tatgccagcc tggaaaaatt ttttgatctg gccccctggg tgcttttgtt gctgatcccc
     6361 gcgatcagca tgcgtagttt ttcggaagag ttccggagcg gcactttcga gatccttcag
     6421 tcgaaaccgc tcacccaatg gcagattgtc aatgggaaat acctggccgc cctattggtt
     6481 atcggtatcg ctttgatccc cacctggatt tactattttt ccgtacagca actcgccgcc
     6541 gggacgggaa tggacaccgg agccgctttc ggggcttata tcggactctt tctgctgacg
     6601 gccgtttttg cggccatcgc gctttgctgc agcagtttca ccaccaattc cattattgcc
     6661 tttatcgctt cgggcttttc ctgcttcctg atctataccg gttttcacgc catcagccgg
     6721 atttcttctt tacccgcagg gttcgattat tatattgaca tgctgggtat tgattttcat
     6781 taccgcaata tgagccgcgg actgatcacc atgcgcgatg ccgtttattt catcagcgtg
     6841 atcctgtttt ttctgatcct cacaaaaaac aatctgaata aaagatagcc tgacctatga
     6901 acaagatctt ctccttcaat tcaggctggc tctttccgct gctgctgtta atactgatca
     6961 atgccggcgc ttccttctgg tccctgcgtt tcgacctcac cagtgaaaaa agatatacgc
     7021 tgagtgcccc ggttaagaaa atgctgaata atattcatga ccaggtgaat atcgatatct
     7081 atctccaggg aaatttaaaa tcgggactga gaaaactgcg gaacagcaca gaagctttat
     7141 tacaggaatt caacgattat gccgatggca atatccatta ccgtatggtg gatccggtgg
     7201 ccggaggaga tgattccacc cgctccatga tcctggattc gctgggaagg atgggtattc
     7261 aacccatgac cctggtcgcc caggccaaaa aaggggaaga acagacccag cgcatcgtga
     7321 ttccggcagc catggtgaaa tataagaacc ggatatttcc ggtagacctg ctcaagggcg
     7381 ttcagcgcag ccgggaaggg cagcctcctg aacaacttta tgtgaatgca gagaccctgc
     7441 tggaatataa atttgccaat gccattgata aactgctgaa agatacgctt cctgccgtgg
     7501 gttatctgat gggaaacgga gagccgctcg acttccgggt ttatagcctg attcaatacc
     7561 tgcgtgaaaa ttaccggttc ggcatgctgc ggttagacag tctcccggta attccgtccc
     7621 gcgtgaatgc tctcattatg gttaaaccct ccagtaaatt caccgatgac gaaaagcgca
     7681 agctcgatca gttcgtagta cacggcggga gcctgatctg catgattgat aatttgcacg
     7741 ccgagccgga tagtttacag gtgagcaaag aagtaattgc ttacgaccgg ggcctggacc
     7801 tcgatgacct cttatttcat tttggcgtcc ggatcaacca ggacctggtg gaggatatgc
     7861 agaatgcgag catcaacctc gttgtcggca gccagggagg gaagccccaa ttacaattag
     7921 aaccctggcc gtactatccc ctgctcgacg gaaacccgtc cagccccatt tcaaaaaacc
     7981 tggatccggt tttttcccgg tttgccaatt ccatcgatac ggtaaaagct ccgggtatcc
     8041 ggaaatccat tctgttacaa agctcgccca acgcaagaac catttcgacg cctgcccgga
     8101 ttaccctgga aagcatcaaa accgccacgg atcccaaggc ctttaaccgg cagaatattc
     8161 ccgtggccgt tctgcttgaa gggaaattcc attctctgta tgccaaccgg atctcaccgg
     8221 aaatggagga cagcctggat aaattttacc accagccttt tttaaggacc ggggaaaaaa
     8281 ctgcccgcgt aatcgtttgc gcggatgccg atgtgctgat gaatgaactc agcgaccgcg
     8341 gccctcttcc cctcggtttt agcaaggaca tcaattatac ctttgccaat atggattttc
     8401 tgcagaactg cctggattac tgtgtgagct cttccggcat catggaggca aggtcgaagg
     8461 actacacgtt gcggctgctg gatccggaaa aaacagagga tgacagaaca ttctggcagt
     8521 ttgtcaacat agtttgtcct ttgttggtta tcctgttagg tgccgttatc tttcatttca
     8581 taagaaagag gaaatacagc atccgctgac aaataaatga tcaattaaat tttccaggtc
     8641 ttgactcatt cccagttcgc gtatttaatt tttggcattg tcctggtgct tgccctggta
     8701 tttgacctgg gcttgctcag caaaaaaggg gcggtcatca cgatcaaaaa ggccttgctg
     8761 cagactgctt tctgggtcat cctcgccatg gctttcttcc tgttcctgtg ggtggacgaa
     8821 aaccggtcca cggcccttga atacctcagt gcgtatctca tggaatggag cctcagcatc
     8881 gataatattt ttgtattcat cttgattttt tcgtttttcg gagtaaagga aaaatttttt
     8941 tccagggtat tgctgatcgg tatcctgctg gccgtttttt tccggatcat ttttattacg
     9001 gcaggcattg tgctggtgga aagatttggc tggctgctgt atatcttcgg agccattctg
     9061 ttatataccg ggcttactat gttttttgcc aaaaaagata aggggaccaa cctggaagaa
     9121 aataaggtct accgcttcct gaaacgcttt cttccgttaa catcggaaga tggcggcgga
     9181 aaactccgca ctcggataaa cggcaaaaaa ttttatactt ccatctttgt cgttattgtc
     9241 ttgttggcca ctacggatat cgttttcgcg ctggactcca ttccggccgt gtttggcata
     9301 acccagaaca ggctcgtgat ctatacatcg aatatttttg ccgtgctggg gcttcggtcg
     9361 ctgttttttc tgctgcgggg cgccgtggac aaattccgtt ttctccagca gggaattgcg
     9421 accgtgctgg tctttatcgg cctgaaaatg ctcagcgaac tcatccatat aaaagtctcc
     9481 gtgctgattt ccctggtggt catcctggtg tgcatattga catccattgt ggtttcgctg
     9541 gtctgggata aaaagcatcc ttcttcctaa aatcaatttt aacattgggt tattctatcc
     9601 ggcatatcgc ttctgttata aatgggaatt ttctgcaatt ccggcaggat gatccggttg
     9661 aatatttgct gacggacagc cgccgcctgc tttttccgga aacttcatta ttttttgcct
     9721 tatatggtcc ccggagagat gcccattctt ttttgccgga cctgtacaag cgcggactgc
     9781 gcaattttat cgtttaccgc aaagtggaaa taaaagattt tcccgatgcc aatattgtac
     9841 tggtcgggga tagcctgaag gcgctgcaga tgctggcggc attccaccgc cgccggttcg
     9901 attaccccgt aatcggcatt accggcagca acggcaagac cattgtaaaa gagtggctga
     9961 acgagttgct ggaagataaa tatcaaatcg taagaagccc gcgcagttac aattcccaga
    10021 tcggtgttcc cctcagcgtc tggctgatga atgaaaccca taccctggcc ctgctggaag
    10081 caggtatttc caaaacgggc gaaatgaagg cactggaaga aatcattcat ccgggcattg
    10141 gcgtgttcac caatattggc ggggcgcatg atgaagggtt tgaaaacacc gaggctaaaa
    10201 tacgggaaaa gctgctgctt tttgaagcgt ctgacaggct gatctatccg tccgatgata
    10261 aagtcctgca tcaaacgatc ctgaactggc agcaatctca ttcggcagta aaattatttt
    10321 ccttcggaaa aaaaggctcc ggcctgcagg tgatcgccca aaagaaaaac cccgacagca
    10381 cggagctgac gctttcttac gaaggaaaga cctggccgct gcttctcggg tttgtgcatg
    10441 atgcggccat tcagaacaca ttaacctgca taacggttct tttatatctg ggcatttcac
    10501 cggaagacct ctccagcaga ttgctgcgtc tcagcccggt ggccatgcga atggaaatcc
    10561 agaccgggat caaccactgt tccgtgatca acgacagcta cagcgcggat atcagttccc
    10621 tgaaaatggc gcttgatttt ttatcccgcc agcagcagca cccgaaacgt accgttatcc
    10681 tgtcggatat cctggaaagc ggacgcaagc cggaaggcct ctaccaggag gtagcggtct
    10741 tgctgaaaaa attcaaagtg gaccggctga tcgcgatcgg cggacaaatg ctggcaaacc
    10801 ggaacctgtt tgccggcagc ggagtgaaag agcaggaatt tcatccttcc accgacctct
    10861 ttctttcctc ctttcagccg cacgctttcc gggaggagac cattctgctg aaaggagctc
    10921 gggtgtccgg tttcgagcgt atagatcagc tgctttccaa gcaggtacat gaaacgctgc
    10981 tggaagtgaa cctggaagcg atggcccata acctgaagca gtaccagcag ttgctggagc
    11041 cttccacccg ggtgatggct atggtcaagg cattctccta tgggagcggc ggctttgaaa
    11101 ttgcgcgcct gctggaattt cataaagtgg attacctggc cgttgcttat gctgacgaag
    11161 ggatttctct tcgcaacagc ggaattcggt tacccatcat cgttatgaat acggaagaat
    11221 cggcaatgga tgctttactg gaatatcagc tggaaccggt gctgtattcg ttcagcatcc
    11281 tccgcgcgat ggataagcgc ttaaaacggg aagcgattgg tttgttcccc gtacatatcg
    11341 aactggaaac ggggatgaac cggttgggct tttccgccaa agaaattccg gaattgctgg
    11401 aaagcctggc ccatgcttcc tttcatgtaa agtccgtttt cagtcacctg gctgccagcg
    11461 aagccgcaga ccaggatggg ttcacgcaaa agcagtttca gcgataccaa tccatgctgg
    11521 ggcaactgca acccggcctg ccgcgtccct tcctgaaaca catttcaaat tccgcagcgg
    11581 tggtcagaaa cccggaattc cagctcgata tggtccggct cggtattggc ctttacggca
    11641 tcgatccctc ggcctccaaa cggcttgaat tgaaaaccgt gggaaccctg aagactacca
    11701 ttgcgcagat caaatccgtt gctgccgggg aaaccatcgg atataaccgg aagggcgtgg
    11761 ccgatcatcc gatgcagatt gccaccgtgc gactgggata tgccgacggg tatccgcggt
    11821 ccctgggcaa cggtacggga aagatgtggt ataaaggacg gctggtgcct accatcggaa
    11881 atctttgtat ggatatgacc atgctcgata ttaccggtat tgaaaacgcc agggaagggg
    11941 atgaactgat cgttttcgga cccgaacttc cggtggaaca ggtggctggc tgggccggga
    12001 ccattccgta tgaactgctg gccggcattt cccaacgggt taagcgggtt tattttgaat
    12061 aagcgctttc tttaggaaat cttaaggggt cgttcttttg gtaacttatt atatttgcgg
    12121 ctcattatga atatcaaaga aaggaaggtc ctttttatcg tattgctgat tattgttgcg
    12181 gatcaggtac tgaagatatg ggtaaagaca catatgcccc tgagtcataa ctgggcagcc
    12241 tactatcgct tacccgtttc cccttacgaa aacggcatcc aggtgctggg taaaaaattc
    12301 cagttgttct ttgtggaaaa tgaaggcatg gcctggggct ggaaatttgg cgggaacctg
    12361 ggtaagatgg cccttacttt attccgactg gctgccgtta tttttggagt atattatatc
    12421 cgcctcctga ttaagaggaa ctatcacaac ggatatatca tctgcgcggg actgatcttt
    12481 gccggtgcgc tgggcaacct gatcgacagc atgttctacg gccttatttt tcaggaaagc
    12541 acgtacgacc atgtggctaa aatatttcct tcgcacggct atgccggatt tctgcacggc
    12601 cgggtcgtgg acatgcttta tttccccctg atcaaaacac attacccgca ctggttcccc
    12661 tttgtcggcg gcgaagaatt tgaattcttc agtcctgtct tcaatattgc ggatgcctcc
    12721 atctctatcg gggtgatcgt tctctttctt tttcagaagc ggtttgtaaa gaaacaggcg
    12781 gaaccggaaa cgcaggatgc ggatcatctc cggcttaaaa ccgaagagga aacgaggaat
    12841 ccggaacccc tgacgccaaa cccggaacag ggattgtagg cagtgcataa cttgttaatt
    12901 atcaataatt tatatttcaa tattccgcgt ttaatacggt ccccgcaggt agtttttcta
    12961 taccaaagcc ccgtctgatt ttgtatgttt acgggtagca ggttcatcca aataccctgt
    13021 ctatccgcaa atccgatacc gatgaaaaag ttccttccgg ttctttcatg cctcctatta
    13081 tcttttatcg ggtacggcca gatcgtttca ttcaatggag aatatttcaa gatagatacc
    13141 ctgtatcccc gctatgatat tccttcccac aacaaggggc ttagttttcc ctgggaaatt
    13201 acctatggcc cggatgattc gctctgggtt acggaagccc accgctaccg catctggaaa
    13261 attcatccgg ggaataaagg aaaccggatc ttactggatt tggaaggact caccacattt
    13321 aattattatg atcctccgaa tggatatccg gctccgcagg gcggactcat ggggctggcc
    13381 attcatccgc ttctgctttc cgggaaacct tatgtgtatg ttgccatggt atacgatgtt
    13441 tatccaacca gcggctcacc caataacaat agctgcgggg gcagcggttc ggtgaccaac
    13501 aacccctgtt tttacaaaac aaagatcatt cgctatacct ataacctggt ttcgcattcc
    13561 ctgggttcac cggttatcgt actggatgat ctcatgggaa gcaacgacca taattccgga
    13621 aggctcaaag tgagcccggt acccgagagc gatggcaaat atcatttata ctacacgatc
    13681 ggtgaccagg gggcgggtca gtttaacaac ggcttccgga ccaataacgc acagaataaa
    13741 gatgtatatg agggaaaggt cttgcgcctg aat
//
LOCUS       DASYCU010000010         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1887210, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000010 DASYCU010000000
VERSION     DASYCU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887210"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VGM24_00245"
     CDS             <1..1219
                     /locus_tag="VGM24_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4153813.1"
                     /translation="AVTDARHLFLPSLKANEQIDLGTDNSIPGSYLSYGIIPSTSSGV
                     RKGNYAQSATGNIAILSAQYDLVDFGYRRAYIEKARSYVDLYQADREKELYTLKIQAA
                     RLYFNLLKNQFRLEVDKQNMERYENIFNVIRALTISGIKPGSDSSLAKAELSKSRITY
                     NQTTGQINQLKQELSFLSGIPARNLQIDTSTATYIRIRSEFSIQSPDSSRNPFLAYYE
                     SQKNIFLAQQHLISKAYLPKIVLTADGWARGSGISYNDEFNSFSQGLGYQRFNYLAGL
                     SFQYDLFNGLHRRDKLRVYQFETTASDEALQQEKLALHSAYLRAEGAIRTSETNLAEL
                     PVQLSAATDVYNQKLAQYKAGLINLVDLTNAAFVLDRSQNDFIETLADWYLAQVDRSW
                     ALGKLDSFINLIK"
     gene            1225..>2570
                     /locus_tag="VGM24_00250"
     CDS             1225..>2570
                     /locus_tag="VGM24_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4153814.1"
                     /translation="MVRAALKIPITVLVLFMGLLLFSIAAIRTIPIDIFPRLNLPAIY
                     VIESYGGMSPQQMEGFFSTRMMDQFLYVNGIKNIESKNIQGLTLIKLSFYETTDMAEA
                     SAQVALQVNRASKFFPPGALPPQVIRFDASSLPVGQLVFSSKTKTLKEIYDLVATRIR
                     PMFASVAGLSAPPPFGANSRSIVVNVDPEKLRRFNISPDQVVDAIAKNNSMTPAGNLR
                     IDSVMYVTTVNSLEDNVQEFQDIPIVSNGSSTVFIHDIATVADASDVTVDYALINGKR
                     SVYIPVVKTAAASTWDVVQQLKSKLPEMKNLLPDDVRISYEFDQSVFVINAVKSLMTE
                     GVLGAILTGLMVLLFLRDWRSSLVVIITIPVAILSSVLFLNLFGQTINIMTLSGLALA
                     VGILVDQATVTIENIHQHLEMGKPKRQAIYEACEEISFPLLLILLCILAVFAPALI"
BASE COUNT          629 a          679 c          625 g          637 t
ORIGIN      
        1 ggccgttacg gatgcccgcc atttattttt gccttctctg aaagccaacg agcagatcga
       61 tctgggcacc gacaattcca ttccgggcag ctatctttcc tatggcatca tcccttccac
      121 ttcttccgga gtaaggaaag gaaattatgc gcagtcagcc accgggaata tcgccatcct
      181 gtctgcccag tatgacctgg ttgattttgg ttaccgccgg gcctatattg aaaaggcaag
      241 gtcttatgta gacctgtacc aggcagaccg cgaaaaggaa ctctataccc taaagatcca
      301 ggcagcccgt ttatatttca atctcctgaa aaatcagttc cggctggaag tggacaagca
      361 gaatatggag cgttatgaaa atatcttcaa cgtgatccgt gcactgacca tttcgggtat
      421 aaaacccggt tccgattcat ccctggcaaa agcggaattg tcaaaaagcc ggatcaccta
      481 caaccagacc accgggcaaa tcaaccagct taagcaagaa ctttcttttt taagcgggat
      541 acccgcccga aacctgcaga ttgacacatc cacggcaacc tatatccgta tccggtcgga
      601 attttccatt caatcccccg acagcagccg caatcctttt ctggcctatt atgaaagcca
      661 gaagaatatt ttcctggccc agcagcatct gatcagcaag gcataccttc caaaaattgt
      721 gctgacggct gatggctggg caaggggctc aggcatttct tacaacgatg agttcaattc
      781 cttttcccag ggattggggt atcagcgttt caactacctg gcggggcttt cttttcaata
      841 cgatctgttc aacggtttac accggcgcga caaactccgg gtgtatcaat ttgaaaccac
      901 ggccagcgac gaggccctgc agcaggaaaa gctggcgctg cattcggcct atctgagggc
      961 cgaaggcgcg ataagaactt ccgaaaccaa tttagcggag ctgcccgtcc agctgtcggc
     1021 tgccacggat gtctataatc agaaactggc gcagtataaa gccggtctga tcaacctcgt
     1081 ggatctgacc aatgcggcct tcgtcctgga ccgttcacaa aatgatttta ttgaaaccct
     1141 ggcagactgg tacctggcgc aggtggacag atcctgggcg ctcggaaagc tggattcttt
     1201 catcaatcta ataaaataac cctaatggta agagcagcgt taaaaatacc catcaccgtt
     1261 ctcgtgctgt ttatgggatt actcttgttc tccatagcgg ccatacgcac cataccgatc
     1321 gatatttttc caagactgaa tctgcccgct atctacgtaa tcgaatccta tggcggcatg
     1381 tcgccgcagc agatggaagg atttttctcc acccgcatga tggatcagtt tctgtatgtg
     1441 aatggaataa aaaatatcga gagtaagaat atccaggggc tcacgctcat caaactttcc
     1501 ttctatgaaa ccacggatat ggccgaggct tcggcccagg ttgccctaca ggtaaaccgg
     1561 gcttccaaat tttttccgcc gggcgccctg cctccacagg tgatccgctt tgatgcttca
     1621 tcccttccgg tggggcagct ggtgttcagc agcaaaacca aaaccctcaa agagatttat
     1681 gacctcgtgg ccacccgcat ccggcctatg ttcgcttcgg tggccggttt atcggctcct
     1741 ccgcctttcg gcgccaattc ccggtcgatc gtggtgaatg tggatccgga gaaactgcgc
     1801 cgctttaata tttctcccga tcaggtagtg gatgccatcg ccaaaaataa ttcgatgaca
     1861 ccggccggga acctgcgcat cgacagcgtg atgtatgtga ccacggtaaa ctccctggaa
     1921 gataacgtgc aggaatttca ggacattccc attgtcagca atggctcttc aacggttttc
     1981 attcatgata tcgccacggt ggccgatgct tcggacgtta cggtggatta tgcgctgatc
     2041 aacgggaaac gctccgttta tatcccggtc gtaaaaacgg cagcggcttc cacctgggat
     2101 gtggtgcagc aattgaaatc gaagcttccg gaaatgaaaa acctcctgcc cgatgacgtg
     2161 cgcataagct atgaatttga ccagtccgtg tttgtgatca atgccgtaaa aagtctgatg
     2221 accgaagggg tgctcggggc cattctcacc ggattgatgg tattgctctt tttgcgggac
     2281 tggcgaagca gcctggtcgt gatcatcact attccggtgg ccatactgag cagcgttttg
     2341 ttcctgaacc tctttggcca aacgattaat atcatgaccc tcagcgggct ggcgctggct
     2401 gtagggatac tggtggatca ggctaccgtc accatcgaga atatccatca gcacctggaa
     2461 atgggcaaac ccaagcgaca ggcgatatat gaagcctgcg aggagatatc ttttccgttg
     2521 ttgctgatat tgctctgcat attggctgta tttgccccgg cgctgatcat
//
LOCUS       DASYCU010000011         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5076012, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000011 DASYCU010000000
VERSION     DASYCU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5076012"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1412
                     /gene="murD"
                     /locus_tag="VGM24_00255"
     CDS             72..1412
                     /gene="murD"
                     /locus_tag="VGM24_00255"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY4153815.1"
                     /translation="MLKRMIILGAGESGVGAAILASQKGYSVFVSDGGKIPEENKKTL
                     EERGIEYEEGKHEEKKILEGTEVMKSPGIPEKAAIVKQIRSKGIPVISEIELAYRYKG
                     ESRIVGVTGSNGKTTTTSLTYHICRQGGLDCALAGNIGISFARQVAENPKPWYITEIS
                     SFQLDDIKTFRPDIAILTNITEDHLDRYDYRFENYIRSKFSIVKYQTPEDYFIYNGDD
                     QVIRENLNEYPFQSNPLPITMQQEPNQGGFIRNGQMTLKTGRETVQMSIYDFALKGIH
                     NQYNTLAAGLAAATIGIRKEKIREAVQSFEALEHRMEFVSTVRGVEFINDSKATNVNS
                     TWYALESMQKPVILILGGIDKGNDYSLIRNLVREKVKAIVCMGVDNLKIQEAFKNDVG
                     VMVSTGSAKEAVKSSFHLAEKGDVVLLSPACASFDLFKNYEDRGRQFKEAVRDL"
     gene            1434..>1837
                     /locus_tag="VGM24_00260"
     CDS             1434..>1837
                     /locus_tag="VGM24_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY4153816.1"
                     /translation="MSETISIEKRAFKHLDSRTRGDKVIWAVVVLLALVSLLSVYSST
                     GLLAYKMYKGNTEIYLFKQFAFIIAGVLIIYFAHQVNYIIYSKVAKILFLVSIPLLVY
                     TLFFGVRLNEGSRWIRLPIINLTFQTSDLAKLA"
BASE COUNT          538 a          389 c          501 g          409 t
ORIGIN      
        1 cggattattc atgcttttga aaaaccccca atccgttcgg gtaaagaacg gaggagaaac
       61 ccggaaaggg catgttgaaa agaatgatca tactgggtgc cggtgaaagc ggtgtcggag
      121 cagccattct ggcatcccaa aaaggatatt ccgtatttgt ttcggatggc ggaaaaatac
      181 cggaggaaaa taagaagacg ctggaagaac ggggtatcga atatgaggaa gggaaacatg
      241 aggagaaaaa gatactggag ggaacggagg tgatgaagag cccggggata ccggaaaagg
      301 cggccatcgt aaaacagatc cggtccaaag gcattccggt gatcagtgag attgaactgg
      361 cctaccggta taaaggagag agccgtattg tgggcgtcac cggaagtaac gggaaaacca
      421 ccactacctc tttgacatat catatttgca ggcagggagg gctcgattgt gcgctggccg
      481 gaaatatcgg gatttcattt gcgagacagg tagcggagaa tccgaaaccc tggtatatca
      541 cggagatcag cagttttcag ttggatgaca tcaaaacgtt caggccggat atcgcgatac
      601 tgaccaatat cacggaggat cacctggaca ggtacgatta ccggttcgaa aactatatca
      661 ggagcaagtt tagcattgtt aagtatcaga ccccggaaga ttattttatt tataacgggg
      721 atgatcaggt gatcagggaa aatttaaacg agtatccatt tcaatctaac ccactaccaa
      781 ttaccatgca acaggaacca aaccagggag gtttcatccg gaacggccag atgacattaa
      841 aaacgggaag ggaaacagtc caaatgagta tatacgattt tgctttgaag ggaattcaca
      901 atcaatacaa caccctggca gccggtctgg ccgcggcgac catcggcatc cggaaagaaa
      961 agatccgcga ggccgtgcag agttttgaag cgctcgaaca ccggatggaa ttcgtcagca
     1021 cggtcagggg cgtggaattc atcaacgaca gcaaggcaac gaatgtcaat tccacctggt
     1081 atgcgctgga atccatgcaa aagccggtga tcctgatcct ggggggcata gacaagggga
     1141 acgattattc cctgatccgc aacctggtcc gggaaaaagt aaaggccatc gtctgcatgg
     1201 gggtggataa cctgaaaatt caggaggcgt ttaagaacga tgtcggcgtt atggtcagta
     1261 ccggaagcgc aaaggaagcg gtgaaaagca gttttcacct ggcggaaaaa ggcgatgtgg
     1321 ttttgctgag cccggcctgt gccagttttg atttatttaa aaattatgaa gacaggggaa
     1381 ggcagttcaa ggaagctgtc cgggacctgt gaagaaatta aaaaaaatag atcttgtccg
     1441 aaaccatttc catagaaaag cgagccttta aacacctgga ttccagaacc cggggggata
     1501 aagtgatttg ggccgtcgta gtgctgctgg ccctggtttc cttattgtcg gtttacagct
     1561 ccacgggcct gctggcctat aaaatgtaca agggcaatac ggagatctat ctctttaaac
     1621 aatttgcatt tattattgcc ggggtgctga tcatttattt cgcccaccag gtgaactata
     1681 tcatttattc caaagtggcg aagatcctct tcctggtttc cataccgttg ctggtctata
     1741 ctttattttt cggggtgcgg ctgaatgaag gcagccgctg gatcaggctg cccatcatca
     1801 acctgacatt ccagacctca gacctggcca aactggc
//
LOCUS       DASYCU010000012         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_148545, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000012 DASYCU010000000
VERSION     DASYCU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148545"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGM24_00265"
     CDS             <1..314
                     /locus_tag="VGM24_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153817.1"
                     /translation="VNAKLPRLLQYSAPTWNYENNSFWLQDASYLRVKNIQVGYSFPD
                     RLVGRIKFVKGIRLYVNGTNLFTFTKFQGLDPERFPTNTTGANQYPNIRILTGGVNIK
                     F"
     gene            332..1894
                     /locus_tag="VGM24_00270"
     CDS             332..1894
                     /locus_tag="VGM24_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4153818.1"
                     /translation="MKRRLIYFLVCSVMLSGLPACKKDFFDLYPRDQLSPGSFWKNQD
                     DAMAALTGCYNSLWNVTANVLPYMDVLTPNAYSEYPWEGWKDISKSTQTSEAPAGPAT
                     VWEGAYRGIGRTNTFLANIDKPEMDETIRARMKSEALFLRAYYYFTLADHFGGAPLIT
                     DPPDVSQATLARSPREDLIAQVLKDLDDAEPGLPLTVPSAENGRATQGAALALKARVL
                     LYDSKWDEAAAAAKAVMDLNVYQLFPSYRGLFLPQNENNSEVIFDVQFKQPEYPSAWD
                     TYLGIYDGNILTAAIGWSSIEPTTDFVDEYEMKDGTVWSPTNPIADPADKYNNRDPRM
                     DQTIFRKGKTYQGLPYPVDPDGYPGIYTGFSFKKYTVYDNAPGPQIDYNKSEINGIIL
                     RYADVLLMYAEAKNEVSGVDQSVYDAVNAVRTRAGMPGLPAGLSQDQMRDRIRHERRM
                     EFVIEGLYYSDVRRWKIAESVLNRDVELDGSYKRGIMDERTFNPAKDYVWPIPQREID
                     YSQKAIVQNPNY"
     gene            1934..>2496
                     /locus_tag="VGM24_00275"
     CDS             1934..>2496
                     /locus_tag="VGM24_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153819.1"
                     /translation="MKNLFFFLFLFLLSVRSDAQEEAGQVLITLNEKDSGRVFEGIGG
                     VSAGASSELLIDYPETQRKEILDYLFKPGFGASLQQLKVEIGADVCVVGAEASHARNL
                     NELRYPKKEYYERGYEYWLMKEAQKRNPGMIFCALEWGIPSYLTGFWTRENAAYITQF
                     VKGAKDFLGINMKYISPGKNESTIDGN"
BASE COUNT          682 a          574 c          645 g          595 t
ORIGIN      
        1 acgtgaatgc gaaacttccc aggctactgc agtacagcgc ccccacctgg aattatgaaa
       61 ataacagttt ctggttacag gatgcttcct acctgagggt gaaaaacatt caggtcggtt
      121 attcgtttcc ggacaggctg gtcggcagaa tcaaatttgt gaaaggcata cggctctatg
      181 taaacggcac caatctgttc acgttcacca aatttcaggg cttggatccg gaaaggtttc
      241 ccaccaatac aaccggtgca aatcagtatc ccaacatccg cattttgacc ggcggagtca
      301 acatcaaatt ctaatcatta aatcctggtt tatgaaaagg cgtttaatat atttcctggt
      361 atgttccgtt atgctctccg ggctaccggc atgcaaaaaa gatttctttg atctgtaccc
      421 tcgggatcag ttatctcccg gatctttctg gaagaaccag gacgatgcga tggccgcatt
      481 aacgggatgt tacaactcgc tctggaatgt aacggcaaat gttttgccgt atatggatgt
      541 gctcacgcca aatgcataca gtgaatatcc atgggagggc tggaaagaca tcagtaagag
      601 cacgcaaacc tcggaagctc cggccggacc ggccacggtt tgggaaggtg cctaccgcgg
      661 catcggaaga acaaatacct ttctggccaa tatagataaa ccggaaatgg atgagacgat
      721 ccgtgcccgg atgaagagcg aggccttatt tctgagggca tattattatt ttaccctggc
      781 agatcatttt ggcggcgcgc ctctgattac cgaccccccg gatgtttcgc aggcaacatt
      841 ggccagaagc cccagggaag atctgatcgc ccaggtctta aaagatctgg acgatgcgga
      901 gcccggcctg ccgctcactg ttccgtctgc agaaaacggt cgcgccaccc agggtgcagc
      961 gcttgcctta aaagcccggg tattattata tgacagtaaa tgggatgaag ccgccgctgc
     1021 cgccaaagcg gtcatggacc tgaacgtata tcagctgttc ccttcctacc ggggcctgtt
     1081 ccttccgcag aatgaaaata attcagaagt tatttttgat gtgcagttca agcagcctga
     1141 atatccttct gcctgggata cctatttagg aatttatgac gggaatatcc tgacggcggc
     1201 catcggctgg agctctatag agccgaccac ggattttgta gatgaatatg aaatgaagga
     1261 cggcacggtc tggagtccca caaatcccat tgccgatccg gctgataaat ataacaaccg
     1321 ggatccgagg atggatcaga cgattttcag aaaagggaaa acctaccagg gactgcccta
     1381 cccggttgat ccggacggtt atcccggcat ttatacgggc ttcagtttta aaaagtatac
     1441 ggtatatgat aatgcgccgg gaccgcagat cgattataac aagtcggaga tcaacgggat
     1501 cattttgcgt tacgccgatg ttttactgat gtatgcggaa gcgaaaaatg aagtgtccgg
     1561 tgtggatcag agtgtatacg atgcagtaaa tgcggtccgg accagagccg gcatgcccgg
     1621 cctgccagcc ggtttgagcc aggatcagat gagggatcgt atccggcatg aaagaaggat
     1681 ggaatttgta atagaaggac tttactattc cgatgtccgg cgatggaaaa ttgctgaatc
     1741 ggtgttgaac cgggacgttg aattggacgg atcttataaa agaggaatta tggatgaaag
     1801 aactttcaac ccggccaagg attatgtctg gcccattccc caaagggaaa ttgactacag
     1861 ccagaaagca atcgtgcaaa atccaaatta ctgataccgc gtacccgata catattctga
     1921 aaaaaaatac ctgatgaaaa accttttttt cttcctgttt ctttttctcc tttcagtgcg
     1981 gtcggacgca caggaagaag ccgggcaggt gctgattacc ttaaatgaaa aagactccgg
     2041 gcgcgttttt gaaggcatcg gaggcgtcag cgccggcgct tcttccgaat tgctcattga
     2101 ttacccggaa acgcaacgga aggaaatcct cgactatctt tttaaacccg ggttcggcgc
     2161 ttcccttcag cagttaaaag tggaaatcgg cgctgacgta tgtgtggtgg gcgccgaagc
     2221 aagtcatgcc cggaacctga acgaattgag gtacccgaaa aaagaatatt atgaacgggg
     2281 atatgaatac tggctgatga aggaagcgca gaaaaggaat ccgggaatga ttttctgtgc
     2341 gctggaatgg gggatacctt cctatctgac cggtttctgg acccgcgaaa atgccgctta
     2401 tatcacccag ttcgtgaaag gggcaaagga tttcctgggc atcaatatga aatatatttc
     2461 tcccgggaaa aacgaaagta cgatagacgg gaactg
//
LOCUS       DASYCU010000013         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2322029, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000013 DASYCU010000000
VERSION     DASYCU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322029"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /gene="fucI"
                     /locus_tag="VGM24_00280"
     CDS             <1..692
                     /gene="fucI"
                     /locus_tag="VGM24_00280"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841633.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEY4153820.1"
                     /translation="DVRTYWSPDAVKRVSGWEPAGVAANGFIHLINSGACTLDGSGRQ
                     NRNGKPSMKPFWEITPEESRDCLQATTWYPANNGYFRGGGYSSKFVSRGGMPVTMCRL
                     NLVKGIGPVLQIAEGETIELPEKVHKILDERTDRTWPTTWFVPRLTGKGYFKDVYSVM
                     AHWGANHGAISYGHIGHDLISMASMLRIPVCMHNVAEENIFRPAAWTAFGSDPEGADY
                     RACETYGPLYS"
     gene            831..2177
                     /gene="fucP"
                     /locus_tag="VGM24_00285"
     CDS             831..2177
                     /gene="fucP"
                     /locus_tag="VGM24_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEY4153821.1"
                     /translation="MNLRKGNKTPGLLSAGDGSSYLRPFIMITSLFLLWGFAHGLLDV
                     LDKHFQDTLHVSKMESGFVQFSLYIGYLVMAYPAGLFMKRYGYKKGIILGLSLFALGA
                     FLFYPAAKLAEFIPFLMALFIIACGLCCLETAANPYTTVLGPPAGAARRINIAQSFNG
                     LGWILGPLMGGLFIFGAGQTGNAGTFDSLLKPYMWVGSIVVLVAIVFAITKLPTIQEE
                     NADGIDANALTRELLKHPAFVLAVVAQFLYVAAQTGVNSFFINYVTETLTGVQATISA
                     MMEHLGRFGEFFMPKNSEQAASLILALGGMGSFWIGRLTGAYLMKFIRPQRLLAIYAV
                     INTFLTAIVVMRLGWISVIALFSTYFFMSLMFPTIFALGLRNLGPLTKKAASFLVMAV
                     AGGAFCPPLMGAIADHFGMSVAFLIPLGCFAFIAYYAISRKRTAGEEVNQPVLIHH"
     gene            2189..>3280
                     /locus_tag="VGM24_00290"
     CDS             2189..>3280
                     /locus_tag="VGM24_00290"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HEY4153822.1"
                     /translation="MDKHQKRRAFLKTLTLGSLGAAAPIQILKAEEKLSAERKTDLPF
                     SEEAVVKRKYNGPYKGSHLNRFAFPIGGLGAGMFCLEGTGAISHMSVRNKPEVFNEPA
                     MFAAISVKDLKNGAKVLEGQVPEWKYFGRSGSGNGLAGSTLGLPRFKSAECTTRFPYS
                     TINLQDSELPLHVRITGWSPFIPTDEDNSGLPVGALEYHFANSGTEKLEAVFSYNSKN
                     FLAGGEHAVNAIEKISNGFVLSQQGTKDRPQLQGSFAIFTDDDHAVIDHCWFRGGWWD
                     PLTMAWNNIREARVQAVDPVEKDAGGASLYIPFSLLPGTSKTIRLMMSWYFPETDLHI
                     GTIVTDEKKNCDPESGCCAGPSDLGVVNGK"
BASE COUNT          799 a          756 c          866 g          859 t
ORIGIN      
        1 ccgatgtgcg cacttactgg agtcccgatg cagtaaaaag ggtatccggc tgggagcctg
       61 ccggtgtcgc agcaaatggg tttattcatc tgattaactc cggcgcctgc accctcgacg
      121 gatccggaag gcagaacaga aacgggaaac cgtccatgaa gccattctgg gaaattactc
      181 ctgaggagtc gcgggactgt ctgcaggcaa cgacctggta cccggcaaac aatggttatt
      241 tccggggagg cggatattct tccaaatttg taagtcgcgg gggcatgccc gttaccatgt
      301 gccggctgaa tctcgtgaaa gggattggcc cggtattgca gattgcagaa ggggagacca
      361 ttgagcttcc ggaaaaagtg cacaaaatat tggatgaaag aacggacagg acctggccca
      421 ctacctggtt cgttccccgg ctaaccggca aaggttattt taaagatgta tattccgtga
      481 tggcacattg gggagccaat catggagcca tcagttatgg tcatatcggt catgacctga
      541 tctccatggc ttcgatgctg cggattccgg tatgcatgca caatgtggcg gaggaaaaca
      601 tatttcgccc ggctgcgtgg acagcctttg gatccgatcc ggaaggggcc gattaccgcg
      661 cctgcgaaac ctatggaccg ttatattctt aagttcgtcc ggtacacaag gaatgcatat
      721 agggaatccc ggaatattcc ggtcgtatac atgaatggat gagcaggatg cggataatga
      781 tttaaattta gaattgactg aaaagccatt gaaatattaa taccgtaaat atgaatctga
      841 gaaaaggaaa taaaactccg ggcctgctta gtgctggtga cggatccagc tatctgaggc
      901 cattcataat gatcaccagt ttgttcttac tctggggatt tgcacatgga ttgctggatg
      961 tactcgacaa acactttcag gatacgctgc acgtatcaaa aatggaatcg ggctttgttc
     1021 agttttccct gtatatcgga taccttgtaa tggcttatcc cgcaggatta tttatgaaac
     1081 gttatggata taaaaaaggg attatcctgg gtttatctct gtttgccctt ggcgcatttt
     1141 tgttttaccc tgcagcaaaa ctggcagaat ttattccttt tctgatggcg ctttttatta
     1201 ttgcctgtgg cttatgctgt ctggaaacag ccgccaatcc gtataccacg gttctcggac
     1261 cgcctgccgg tgctgcccgc cgcatcaaca tcgcgcagtc atttaacggc ctgggctgga
     1321 tactgggtcc cttaatggga ggtctgttta ttttcggagc tggacaaact ggcaatgccg
     1381 gaacatttga ttccctgctg aaaccctata tgtgggtggg aagcatcgtg gtgctggtgg
     1441 cgatcgtttt tgcaattacc aaattgccga ccatacagga ggaaaatgcg gatggcatag
     1501 acgcgaatgc acttacccgt gagttgttaa aacatcccgc ctttgtgctg gccgtagtgg
     1561 cgcagttcct gtacgtggca gcgcagaccg gcgtcaactc tttctttatt aattatgtta
     1621 ccgaaaccct tacaggtgta caggcgacca tttccgccat gatggaacat cttggccggt
     1681 ttggtgaatt ttttatgcca aagaattcgg aacaggctgc atcattgatc ctggcgctgg
     1741 gcggaatggg atctttctgg atcggccggt taaccggggc atacctgatg aaatttatcc
     1801 ggcctcaacg cctgctggcg atttacgccg tgatcaatac cttccttacc gcgattgtgg
     1861 tgatgcgctt gggatggatc agcgttatcg ctctgttctc tacttatttc tttatgtcgc
     1921 tcatgttccc gactattttt gcactgggcc tccggaatct cggaccgctt acaaaaaagg
     1981 cggcttcttt cctcgtgatg gcggtggccg gtggcgcgtt ttgtcctccg ctgatgggtg
     2041 ccatcgcaga tcattttgga atgtcggttg cctttctgat tcccttgggt tgttttgcgt
     2101 ttatcgccta ttatgccata tcaagaaaga gaacggccgg tgaggaagtg aatcagccgg
     2161 tattgataca tcattaaatc cggttaagat ggataagcat caaaagcgca gggccttcct
     2221 gaaaacactc acccttggaa gtttgggcgc tgctgcgccg atacagatac tgaaggccga
     2281 agaaaaattg tctgccgaaa gaaaaacaga tctgcctttc agtgaggaag ctgtcgtcaa
     2341 acgaaaatac aatggccctt ataaaggcag tcacctgaat cggttcgctt ttccgattgg
     2401 cgggttgggc gccggtatgt tctgcctgga aggaacagga gccatttccc atatgagtgt
     2461 aaggaataag ccggaggttt ttaatgaacc ggccatgttt gctgcaatct ctgtgaaaga
     2521 tttaaagaat ggcgcaaaag tattggaggg gcaggtgccg gaatggaaat attttggacg
     2581 cagcggcagt ggaaatggcc tggccggctc tactttagga cttccccggt ttaaatcggc
     2641 agaatgtaca acgcgttttc catacagcac gatcaacctg caggacagtg aattgcccct
     2701 gcatgtgcgc attaccgggt ggagtccttt catacccacc gatgaagaca attcaggttt
     2761 accggttggc gcattggaat atcattttgc aaacagcgga acagaaaaac tggaagccgt
     2821 attttcctac aattcaaaaa attttcttgc cggcggcgag catgccgtca atgcaataga
     2881 aaaaatctcc aatggttttg tactcagtca gcagggaaca aaagatcggc cgcaactgca
     2941 gggcagcttt gctattttca cggacgatga tcatgccgtg atcgatcatt gctggttccg
     3001 gggcggctgg tgggatcctc tgaccatggc ctggaataat atccgtgaag cccgcgtaca
     3061 ggccgttgat ccggttgaaa aagacgccgg gggtgcctcc ctttacattc ctttctccct
     3121 attgccgggt acttcaaaaa caatccggct gatgatgtcc tggtattttc cggaaacgga
     3181 cctgcacatc ggaaccattg tcaccgatga aaagaaaaac tgtgaccctg aatccggatg
     3241 ttgcgcaggg ccctcggatc ttggtgtagt caacggaaaa
//
LOCUS       DASYCU010000014         4122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4496884, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000014 DASYCU010000000
VERSION     DASYCU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4496884"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="VGM24_00295"
     CDS             complement(<1..1635)
                     /locus_tag="VGM24_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY4153823.1"
                     /translation="MKNVSKTGVAALLLICLNANAQQADYSIRAVPFTQVKLSDHLWT
                     SHIEINRTVTIPASFERCDKTGRVKNFIMAAKRSGKFCTTFPFDDTDIYKTMEGASYS
                     LAVHPDKKLEAYLDSLITIIGNAQEPDGYLYTARTIDPLHPHPWSGATRWVNEHDNSH
                     ELYNSGHLFEAAVAHFQATGKRNFLRIALKNADLLVNTFGPDKMHVAPGHEVVEMGLV
                     KLYRVTGKKEYLQLAKFFLDQRGKNIKYDKKSKNPWANGSYWQDNEPVVDQEEAEGHA
                     VRAMYLYSAMADVAALTGDSAYLEAIDRIWNNMAADKIYVQGGIGAVPDGERFGADYE
                     LPNATAYNETCAAIGSVMWNQRMFQLHGESKYIDVLEKTLYNNLLSGVGLDGKSFFYT
                     NAMEVKNNLSHPSLERERSGWFECSCCPTNMARFLPSLPGYIYAQHGSDVYVNLFING
                     SADIKIDRRSFQLVQQNNYPWDGRLLFRVNPGEAVPATLRIRIPGWARDQAMPTGLYA
                     FLHPSGGKIELKINGEPAGYKMDRGYAVISRKWKKND"
     gene            complement(1663..2439)
                     /locus_tag="VGM24_00300"
     CDS             complement(1663..2439)
                     /locus_tag="VGM24_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY4153824.1"
                     /translation="MPNAASYQIKLSQFEGPFDLLLFFIERDELDIYNIPIHQLIKDF
                     LAFIHAQEKLNIELSSEFILFVSTLMRIKARMLLPRKELDEQGNEIDPRQELVNKVLE
                     YKRYKEAAARLAELEEDRMQMLKRGNLYRELLQIGEEAGEGTEIQSITLFKLMKTFEK
                     LMQRVQDRNNRPMHTVIQYNYTMEESRSNMIGLLKREGTVAFERIISTCENRIQVIFL
                     FLSILELVQQHYMNILMGEGRNNFILEYNPEGELLQAEEG"
     gene            complement(2562..3500)
                     /locus_tag="VGM24_00305"
     CDS             complement(2562..3500)
                     /locus_tag="VGM24_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HEY4153825.1"
                     /translation="MKKILISLGAVLCAYTSFSQDKEVQELREEATRSIKKDPNDTIP
                     KTWKKGALINFNLNQSSQSNWAAGGEKSSVALNSLFSGYAFYKKGRNSWDNTLDLAYG
                     MVNTSSQGTRKSDDRIDLLSKYGYEVARSWYISALLNVKTQFAKGYAYPDGKEKVLTS
                     DFFSPGYVLLSPGVNYKPNDAFSVFVSPATARLIVVRNDSLASVGAFGVDSGKNSKLE
                     LGAYATISYLKKLGTAAVYTGRLDLFSNYGHKPQNVDVSMTNLLAVKITSVFAMTFAL
                     NLIYDDDVKQVKSDGTTGGAAAQVQELLGIGIAIKL"
     gene            complement(3589..3957)
                     /gene="mscL"
                     /locus_tag="VGM24_00310"
     CDS             complement(3589..3957)
                     /gene="mscL"
                     /locus_tag="VGM24_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY4153826.1"
                     /translation="MSMLKEFRDFAMRGNVVDLAVAVIIGAAFGAIISSLVDDVITPL
                     LLTPALKAANAQNLDQLSWGAVKYGKFLSAIIKFVIIAFILFLIVKAMKSIERKKVPP
                     ATGPSSTDVLLMEIRDELKK"
BASE COUNT         1002 a         1036 c          920 g         1164 t
ORIGIN      
        1 atcattcttt ttccatttac ggctgatgac cgcatagccc ctgtccattt tgtatccggc
       61 cggctcgccg ttaattttca attctatttt cccgccggat ggatgcagaa atgcatagag
      121 accggttggc atcgcctgat cgcgggccca tcccggaatc cggatccgca gggttgccgg
      181 tacggcctcc ccggggttca cccggaataa caagcggccg tcccagggat aattattctg
      241 ctggaccagc tgaaaagatc tccggtcgat ttttatatcc gccgaaccat tgatgaacag
      301 attgacataa acatccgagc catgctgcgc ataaatataa cccggcagcg agggcaggaa
      361 cctggccata ttggttggac agcaggaaca ttcaaaccag ccggatcttt cccgctccag
      421 cgaaggatgt gaaagattat tttttacctc catggcattg gtgtaaaaaa atgatttccc
      481 gtccaatccg accccggata ataaattatt gtataaggtc ttttccagta cgtcgatata
      541 ttttgattcg ccatgaagct gaaacatacg ctgattccac attacgctgc caatggccgc
      601 gcaggtttca ttataggccg tcgcattggg caattcgtaa tctgctccaa accgctctcc
      661 atcgggaacg gcgccaatgc ccccctgcac atatatttta tcagctgcca tgttgttcca
      721 tatcctgtca atggcctcca gataagccga atcccccgta agcgccgcca catctgccat
      781 cgccgaatag agatacatgg ctcttacggc gtgtccttct gcttcttcct gatccaccac
      841 gggttcattg tcctgccagt aactcccgtt tgcccagggg tttttgcttt ttttgtcgta
      901 cttaatattt ttcccccgct ggtccaggaa aaatttagcg agttgcaggt attccttttt
      961 ccccgtaacc cggtaaagtt tcaccaatcc catttccacc acttcgtgac ccggtgcaac
     1021 atgcattttg tccgggccga atgtgttcac cagcagatca gcatttttca aggcgatgcg
     1081 gagaaaattt cttttcccgg tagcctgaaa atgcgcgacg gccgcttcaa acaaatggcc
     1141 ggaattatac agttcatggc tgttatcatg ttcgttgacc cagcgggtgg cgccggacca
     1201 gggatgcgga tgcaacggat caatcgttct tgccgtatac aaatagccat cgggctcctg
     1261 ggcatttcca atgattgtaa taagagaatc caggtaagct tcgagttttt tatccggatg
     1321 cacggccagg gaataagaag cgccttccat ggttttataa atatccgtat cgtcgaaagg
     1381 gaaagtggtg cagaatttac cgcttctctt agccgccata atgaaattct taacccttcc
     1441 cgttttgtcg cagcgttcga aagaggcggg gatggtgacc gtccggttga tctcgatatg
     1501 cgaggtccat aaatgatccg acaattttac ctgggtgaaa ggaactgccc ggatcgaata
     1561 atcggcctgt tgggcattgg cattcaggca aattaagagc agcgcggcca ctcctgtttt
     1621 gctgacattt ttcattcccg gatttttttg ttgaataata gtttatccct cttccgcctg
     1681 cagcagttcc ccttcaggat tatattccaa aatgaagtta tttcttcctt cgcccattaa
     1741 gatattcata tagtgttgct ggacgagttc caggatggaa agaaacagga atatgacctg
     1801 aatccggttc tcacaggtac tgatgatccg ttcaaaagcg actgtccctt cccgtttaag
     1861 caggccgatc atattcgacc ggctttcctc catggtatag ttatactgaa taaccgtatg
     1921 catgggccgg ttgttccggt cctgcacccg ttgcatcagc ttttcaaaag tcttcattaa
     1981 tttgaaaagg gtgatggact ggatttcggt tccttctccg gcctcctccc cgatttgtaa
     2041 taattcccgg tagagatttc cccttttcag catttgcatc cggtcttctt ccagttcagc
     2101 cagtcgtgct gcggcttctt tgtaccgttt gtattccagt accttgttta ccaattcctg
     2161 gcgtggatca atttcattcc cctgttcgtc cagttctttg cgcggcagca gcattcttgc
     2221 cttgatccgc atcagggtgg agacaaacag gataaactca ctgctcagtt caatattcag
     2281 cttttcctgc gcatgaataa aggcaaggaa atctttgatc agctgatgaa tgggaatatt
     2341 gtaaatatcc agttcatccc gttcaataaa aaaaagcagc aggtcaaaag gcccttcgaa
     2401 ctgggataat ttaatctggt aggatgcggc gttcggcatg cgtctgcttt agttacaagg
     2461 atacataaaa atagattccg gacgggcctt tataaccgga gtttgtaaac atttatttca
     2521 ccctgcggaa gctatccgcc cgtttattca ggatttatac cctaaagctt aatcgcaatt
     2581 ccgatcccga gcagttcctg cacctgcgct gcggcgcccc ccgtcgttcc gtcactcttc
     2641 acctgtttta catcgtcgtc ataaatcagg ttcagcgcaa aggtcatggc aaaaacgctc
     2701 gttattttca ccgccagcag gttggtcatg gagacatcca cattctgcgg cttatggccg
     2761 taatttgaaa aaagatcgag cctcccggta tagacggcgg cggtgccaag ctttttcaaa
     2821 taggaaatcg tagcataggc acccagttcc agcttggaat tttttccgga atccaccccg
     2881 aaagctccca cactggccag ggaatcattc cgtactacga tcagcctggc cgtagctggt
     2941 gatacaaata ccgagaatgc gtcgttgggt ttatagttta cgccgggcga gagcagaaca
     3001 tatcccgggg aaaagaagtc ggatgtcaat actttttcct taccgtccgg ataagcatat
     3061 cctttggcga actgggtctt tacgttcagc agggcactga tataccagct cctggcaact
     3121 tcatatccgt atttagaaag caggtctatg cgatcatccg atttgcgggt tccctgactg
     3181 ctggtattga ccatcccgta agccaggtcg agggtgttat cccaggagtt cctgcccttc
     3241 ttgtaaaacg cgtatccgct aaagagactg ttcagggcaa cggaggattt ttctccgccg
     3301 gccgcccagt tgctttggga agactgattt aaattaaaat taatcagggc tcctttcttc
     3361 caggttttcg gaatggtatc gttgggatcc ttcttaatac ttctggtggc ttcctctctc
     3421 aattcctgaa cctctttatc ctgggaaaag ctggtatagg cacatagcac agcccccaga
     3481 ctgattaaaa tttttttcat ctttttttaa aattggtcaa ataataaatt ctctctgatt
     3541 ttctccggcg gggttcttga ttaaggcaaa caatcggagg acgggaaatt attttttcaa
     3601 ctcgtcccgg atttccatga gcaatacgtc ggtgctggaa ggtccggtag ccggcggaac
     3661 tttttttctt tcaatcgatt tcattgcttt aacaatgagg aataaaataa atgcgataat
     3721 gacaaatttt attatggccg acaaaaattt gccatacttg acggcccccc aggaaagctg
     3781 atccagattt tgggcgttgg ctgccttcag cgcaggggtc agcagaaggg gtgtaatgac
     3841 atcatccacc aaagaggata taatggctcc gaatgctgcc ccgataataa cagcaactgc
     3901 cagatcaacc acatttcccc gcatggcgaa atcccggaat tcttttagca tactcatagc
     3961 cgtagtttta aaagtcaaaa aattgaatgc ccaaaattaa taaataccct gatcaaacta
     4021 gtattccaac ccgttacccg ggcattaaac cgacatgatc tccttttctt tggcgttcag
     4081 gtgtttttcc acgagctgga tatgctgatc ggtcagatcc tg
//
LOCUS       DASYCU010000015         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1743768, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000015 DASYCU010000000
VERSION     DASYCU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743768"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1376)
                     /locus_tag="VGM24_00315"
     CDS             complement(105..1376)
                     /locus_tag="VGM24_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEY4153827.1"
                     /translation="MARLGATLFVFVLLANNAFSQMVTRPFPQHVIYHAGCIRPNNIS
                     QKQMDAQVLRFYQNWKSQYIHASPGMDQAYVFFQEEGSKMQSVSEGQGYGMLITALMA
                     GADPVAQGTFDSLYRYVLAHPSKPDSPLMAWSQLLNSHDKDQTSATDGDMDIAYSLLL
                     AHDQWGSSGKYNYLEAARHMINSIMKYEINQKSYNVLLSDAVEYDSEDYFDMRSSDFM
                     PLHFKSFRLATGDAQWNKVVDQNYRLFLQMQDNYSKDAGLIPDFIIHVNDNPRPAKPR
                     YLESRYDGFYNYNACRVPWRIAIDYLLTGDKRSSEIVNKINRWIRQTSSDNPDNISAG
                     YSLEGDDIRSRNYEALSFIGPFAVSAMVNASNQRWLNSIWDYLVRFRLQDFDYYDNSI
                     KMISLLIVSGNYWEPSAPSIAYHAPGENVQG"
     gene            1579..3987
                     /locus_tag="VGM24_00320"
     CDS             1579..3987
                     /locus_tag="VGM24_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HEY4153828.1"
                     /translation="MNKLFSACIRHFYLLIILTAFAGESVAQVHQMPAYPLISHDPYF
                     SIWSFSDTVTATPTRHWTGTDQPLMASLTVDSKNYRILGKEAPRYETVLASAEEKSEQ
                     VKYSETRPSGDWMNPDYRDDDWKTGGTPFGDNMTFAKTKWTSPDLWTRRSFPTREGGR
                     GELFLKIRHDDNVEVYLNGVNVYSVRGWTGKYIYVPVPDSLLKKTGEGNNVLAIHVAN
                     TAGGQWLDAGLVRKLQVAAEPLTGIQKQVTVTATRTIYDLECGPVIVRMSFISPLILN
                     DLHLLSRPVSYIDVRIRSVDLKTHRIQLHFMASANLAVNVPGQEVVCSKLSSTSLDLL
                     KAGTKDQPVLAKKGDDLRIDWGYLYVGAPKKERAVQTISGRELKTDFPPENISDKRKD
                     HLVLLGYDDLYSIQYFHHNLKPWWKLEAGSSIEKEMDAALAGYPSILKKCDSADQQVY
                     QDALAAGGETYARLCVAAYRQSVSAHKLVKSPDGDILFLSKENFSNGCINTVDVTYPS
                     SPLYLMYNPDLLKGMLNGIFYFCESGRYAKPYAAHDLGTYPLANGEIYGEGMPVEESG
                     NMIILTAAIVKKEGNAEFAVKHWKILTQWAEYLLQKGLDPADQLCTDDFAGHLARNAN
                     LSVKAIVALRCFGMMAAMKGDSLVAKRFTNMAEGMVRQWERLADAGDHYALTFNNKNT
                     WSQKYNQVWDKILDLHLFPDSVYRKEINYYLGKQNAFGLPLDSRKTYTKSDWILWTAT
                     LTNNRKDFEALIDPVYKYLTETPTRVPLSDWHETLDGKKVGFQARSVVGGYFMEALYR
                     QGHP"
BASE COUNT         1016 a         1010 c         1075 g          972 t
ORIGIN      
        1 gattaaggtc cggagccagt ctctcataaa ctttaaattt aaggccgaac gccattccgg
       61 tatggtattc aaaaagggtg cctgctcaaa tcttccttac ccaatcagcc ttgcacgttc
      121 tctcccggtg catgataggc aatggaagga gcggagggct cccagtaatt tccggacacg
      181 atcagcaggc tgatcatttt gatgctgtta tcgtaatagt cgaagtcctg gagccggaac
      241 cggaccaaat aatcccagat ggagttcagc cagcgctgat tggaggcatt caccatggcg
      301 gaaacggcaa agggtcctat aaaactcagc gcctcgtagt tccggctacg gatatcgtct
      361 ccttccagcg aataaccggc ggaaatatta tccggattat cggagctcgt ctgccggatc
      421 cagcgattga tcttattgac aatttcagag gaacgcttgt cgccggtgag caggtaatcg
      481 attgcgatcc gccagggaac ccggcaggca ttgtaattat aaaatccatc atatctcgat
      541 tcgaggtatc tcggcttcgc ggggcggggg ttgtcgttga catggataat gaaatccggg
      601 atcaggccgg catccttact gtagttatcc tgcatctgga gaaaaagccg gtaattttgg
      661 tccaccactt tattccattg ggcgtccccc gtcgccagcc ggaatgattt gaaatgaagg
      721 ggcatgaaat cggaagaccg catatcgaaa taatcctcac tgtcgtattc aaccgcatcg
      781 ctgagcagta cgttgtagga tttctggttg atctcgtatt tcatgatcga gttgatcatg
      841 tgccgtgccg cttcgagata attgtattta ccggatgaac cccactggtc atgagccagc
      901 agcagggaat aggcaatatc catgtccccg tccgtcgccg atgtctgatc cttatcgtgg
      961 ctgttcagta attgagacca ggccatcagc ggagagtccg gtttgctcgg atgcgccagc
     1021 acatagcggt ataaactgtc gaaggttccc tgggctaccg gatccgcacc ggccatcagg
     1081 gccgtaatga gcattccata tccctgtcct tcggagacgc tctgcatttt ggaaccttct
     1141 tcctgaaaaa aaacataggc ctgatccatt cccggagagg cgtgaatata ctggctcttc
     1201 cagttttgat aaaagcgcag gacctgggcg tccatttgct tttgggaaat attattgggc
     1261 ctgatgcagc cggcatgata aataacatgc tggggaaatg ggcgggtgac catctgggaa
     1321 aatgcgttgt ttgccagtaa aacgaaaacg aataaggttg ctccaagtct tgccatacac
     1381 gtaaaacggg gtttcagctg ccggattgct tactgcaaga tctgagccct gtttattttc
     1441 cgcctttttt ccctgctttt attattgtta atttttttta aatgaactac cgcacccaaa
     1501 agaaagtcat aaaatctatg gctcctgaaa accagtttgt tgtaatttgg gctccgggaa
     1561 tcacgcatac catcaatcat gaacaaactt ttctccgcct gcattcgtca tttttattta
     1621 ttgattattc tgaccgcctt tgccggggaa tcggttgccc aggtgcatca gatgcctgcc
     1681 tatccgctga tcagccatga tccatatttc agcatctggt cgttcagcga tacggttacc
     1741 gccacgccca cccggcactg gaccggtacg gatcaacccc tgatggccag ccttaccgta
     1801 gacagtaaaa attaccggat cctgggaaag gaggcccccc ggtatgaaac ggtcctggct
     1861 tccgcggaag aaaaatcgga gcaggtcaaa tattcagaga cccggccttc cggggactgg
     1921 atgaacccgg attaccggga tgacgactgg aagaccggcg gaaccccgtt tggcgacaat
     1981 atgacctttg ccaaaactaa atggaccagc ccggatctct ggacccgccg ttcatttccc
     2041 acgcgggaag gtggccgggg ggaattgttt ttaaagatcc gccacgacga caatgtggaa
     2101 gtatacctca acggcgttaa cgtctattcc gtcaggggat ggacgggtaa atatatctat
     2161 gtgccggttc cggattccct gttaaagaaa acgggcgaag gaaataatgt tcttgccatt
     2221 catgtggcca ataccgccgg gggacaatgg ctggatgccg ggcttgtccg caaacttcag
     2281 gtggcagctg aaccattgac cggaatacaa aaacaggtaa cggtaacggc tacccgaacc
     2341 atttacgacc tggaatgcgg accggtgatt gtccgtatga gctttatttc cccgttaata
     2401 ttaaatgacc ttcatttatt gtcccggccg gtttcctata ttgatgtccg gatccgttcc
     2461 gttgatctga agacacacag gatacagttg cattttatgg cttcggcgaa cctggctgtt
     2521 aacgtgccgg gccaggaagt cgtctgctca aaattgtcca gtacttccct tgacctctta
     2581 aaagcaggca ccaaagatca gcccgtgctg gcaaaaaagg gagacgacct tcgcattgac
     2641 tggggatacc tgtatgtggg cgcaccgaaa aaggaacggg ctgttcaaac catatcggga
     2701 agggaactga aaacggactt tccgccggaa aacatttcag acaaacggaa ggaccatttg
     2761 gtgctgctcg gttatgacga tctctattcg atccagtatt ttcaccataa cctgaaaccc
     2821 tggtggaagc tggaggccgg aagcagcatc gaaaaggaaa tggatgccgc gctggccgga
     2881 tatccttcca ttctgaaaaa atgcgacagt gccgatcagc aggtttacca ggatgccctg
     2941 gcggccggcg gggaaaccta tgccaggctt tgtgtggcgg cctaccggca aagcgtatcc
     3001 gcacataagc tggtgaagag cccggacggc gatatcctct ttctttccaa agaaaatttc
     3061 agcaacggtt gcatcaatac cgtggacgtt acttatcctt cctcccccct gtacctgatg
     3121 tataacccgg acctgctgaa gggcatgctc aacgggatat tttatttctg tgagagcggc
     3181 cggtacgcaa aaccttatgc cgcgcatgac ctgggcacct atccgctggc caacggggag
     3241 atttacggag aggggatgcc ggtggaagaa tccggcaata tgatcatcct gacggcggcg
     3301 atcgtgaaaa aagaggggaa tgccgagttt gcggtaaagc actggaagat cctgacgcag
     3361 tgggcagaat acctgctgca gaaggggctg gatccggcag accagctgtg cacggatgat
     3421 tttgcgggac atctggcgcg caacgccaac ctctctgtga aagccatcgt ggcccttcgc
     3481 tgttttggga tgatggcagc catgaaaggc gacagcctgg tggccaaacg atttacgaat
     3541 atggcagagg gcatggttcg tcagtgggag agactggcag atgccggaga tcattatgcg
     3601 ctcacgttta acaacaagaa tacctggagc cagaaataca atcaggtatg ggacaagatc
     3661 cttgacctgc atctttttcc ggattcggtg taccggaagg aaataaacta ttatctcggc
     3721 aaacaaaatg cgttcgggct tccgctcgac agccggaaga cctataccaa atcggactgg
     3781 atattatgga ccgccacact aaccaataac cggaaagatt tcgaagcgct cattgatccc
     3841 gtttataaat acctcaccga aacgccgacc cgcgtgcctt tgagtgactg gcacgaaacg
     3901 ctggacggga agaaagtggg gttccaggcc cgcagcgtgg tgggcggtta ttttatggaa
     3961 gccctgtacc ggcaagggca tccttgacag gaatggccgg cttcttatta tatttgcgcc
     4021 atgcattatg tttctgctga gggcctggga aagtcgttcg gggttaagcc act
//
LOCUS       DASYCU010000016         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5348, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000016 DASYCU010000000
VERSION     DASYCU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5348"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VGM24_00325"
     CDS             complement(<1..183)
                     /locus_tag="VGM24_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153829.1"
                     /translation="MAGKKAFVLRIDETVYKALEKWASDEFRSVNGQIEWLLQTQMKS
                     AGRLKSNPAENPEKQKN"
     gene            complement(195..1064)
                     /locus_tag="VGM24_00330"
     CDS             complement(195..1064)
                     /locus_tag="VGM24_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY4153830.1"
                     /translation="MEKNSKPVSGFIVLILALLLLAATIYFFITGAQQDNTPYAIIGA
                     LLMLALLFISKGFMIVNPNEARVLTFFGKYSGSVKDNGLLWVNPLYRSVRISLRSENL
                     EGTPLKVNDKMGNPIEIATVIVWQVRDTYKASFEVVNYNQYVRVQSEAAVRHLATTCP
                     YDHMEDEHAEITLRDGGDKVNELLEKELNERLAPAGIIIKEARISHLAYAPEIAGAML
                     QRQQATAIVAARFKIVEGAVAMVELALEQLSRKGIVQLDEEKKAAMVSNLMVVLCGEK
                     AATPVLNTGTLYQ"
     gene            complement(1212..2252)
                     /locus_tag="VGM24_00335"
     CDS             complement(1212..2252)
                     /locus_tag="VGM24_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY4153831.1"
                     /translation="MKKIRILSIDGGGIRGILPGTILSYLEAAINKIDPGGPQNIGDY
                     FDMIAGTSTGGILTCLYLMPGANGKARYSASDALNLYLDHGESIFKRSLWEKISSGSG
                     VIHSKYPVSKLESLLLQYCGETTLSDLIKPSLIPAYEMTDRKAVFFTSSDAGQDPIYN
                     FKVRDVARATAAAPTYFRPARISSLSGQIWSLIDGGVFANNPTLCAFAEARKTPFSDF
                     LHDPEKPSSPTARQMLIVTIGTGSVKKPYHYNKMRNAGEIGWLEPVIDILMSGNSETV
                     DYQLRQMFRSLDAADQADYYRLEPGLKEACSEMDIATPENIANLHQAGLTYITDHAGL
                     LNEIAAKIILHA"
     gene            2477..3262
                     /locus_tag="VGM24_00340"
     CDS             2477..3262
                     /locus_tag="VGM24_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4153832.1"
                     /translation="MNISVSGKYAVVCGSTQGIGLAAAGNLAELGANCILIARNESAL
                     VSIVSALPRVGEQHHQYVTADFSDPKQVRRAIESIVRGITVEILVNNTGGPKAGPVMD
                     ALPEAFEEAFRAHLVCNQILAQAVIPGMKASGYGRIINIISTSVKTPLPNLGVSNTIR
                     LAVASWAKSLSNEIAKYNITVNNVLPGLTETGRLQSLVRQSAEAESRTVAEVEGQMIQ
                     SIPMKRFGSPSEIANVIGFLASPAASYVNGTNIAVDGGRTAAL"
BASE COUNT          831 a          789 c          849 g          862 t
ORIGIN      
        1 atttttttgt ttttccgggt tttcggcagg gtttgatttc agccggccgg ctgatttcat
       61 ttgcgtctgc agaagccatt cgatctgccc gttcacactt cggaattcat cggaagccca
      121 cttttccaat gccttatata ccgtttcgtc tatccgtaaa acaaatgctt tttttccggc
      181 cataccacaa tgcattattg gtacaatgta cccgtattca ataccggtgt tgcagctttc
      241 tctccgcata gtaccaccat caggttgctt accatagccg cttttttctc ttcatcgagc
      301 tgaacaattc cttttctgga caattgctca agagccagtt caaccatagc cacggcgcct
      361 tccacaattt taaaacgggc agccacgatg gccgttgcct gctgcctttg aagcatcgca
      421 ccggcaattt ccggagcata ggcgaggtga ctgatccggg cctccttgat aatgataccg
      481 gcaggggcca gtctctcatt taattctttt tccagcagtt catttacctt atctcccccg
      541 tcgcgcaggg taatctcggc atgttcatct tccatatggt cgtacgggca ggtggtggcc
      601 agatgccgaa cggcggcctc gctctgcacc cttacatatt gattataatt caccacttca
      661 aaagatgcct tataggtgtc tctcacctgc caaacaatta ccgtggcgat ctcgatcggg
      721 ttgcccattt tatcattcac tttcaatggg gtcccttcca ggttctccga acggagagaa
      781 atacgaacgg agcggtaaag cgggttaacc cagagcaaac cattatcctt gaccgatccg
      841 gaatattttc cgaaaaaggt gaggacccgc gcttcattgg ggttgacaat cataaaaccc
      901 ttggaaataa aaaggagggc aagcatcagt aaagccccga taatcgcata cggcgtattg
      961 tcctgctgtg cgccggttat aaaaaaataa atggtggcgg ccagcagcag gagggctaaa
     1021 atcaggacga taaaaccgga taccggtttg ctgttctttt ccatgtgttt aaattaaaat
     1081 gatattaaaa tgatatcaaa tatagaaaga tttattgaaa aaggaaaaaa taaaaaatgg
     1141 gaaaaaatac ccggacggcg atgggcgaac cgcaccggaa gtttttgctc tttgggattc
     1201 tgccttcccg gttaagcgtg cagaatgatc ttcgcggcga tttcattcag cagcccggca
     1261 tgatccgtta tataggtaag tcctgcctgg tgaaggtttg cgatattttc cggggtggcg
     1321 atatccatct cggagcaggc ttctttcagt cccggctcca gccggtaata atccgcctgg
     1381 tcagccgcgt ccaggctcct gaacatttga cgaagctggt agtccactgt ttcagaattt
     1441 ccggacatga ggatatcgat aacgggctcc agccaaccga tctctcctgc attcctcatt
     1501 ttgttataat ggtagggctt tttcacagat ccggttccga tggttacgat cagcatctgg
     1561 cgggcagtag gagaactggg tttttccgga tcatggagga aatccgaaaa gggcgttttc
     1621 cgggcttctg caaaagcgca aagcgtgggg ttattggcaa ataccccgcc gtcaatcaaa
     1681 gaccagatct gacccgaaag ggagctgatg cgggcgggcc tgaaataggt tggggcggcg
     1741 gcggtggccc tggccacatc cctgacctta aaattataga tcggatcctg tccggcatcg
     1801 ctgctggtaa aaaataccgc cttacggtcc gtcatctcat aagcggggat gaggctgggt
     1861 ttgatcagat ccgagagggt ggtttccccg caatattgca gcagcaggct ttccagttta
     1921 ctcaccggat atttggagtg gatgacaccg gatccgctgg aaattttctc ccacagggat
     1981 cttttaaaga tggattctcc gtgatcgaga taaagattca gcgcatccga agccgagtat
     2041 ctggctttcc cgtttgcgcc tggcatcagg tagagacagg tgagaatacc tcccgtgctt
     2101 gtcccggcga tcatgtcgaa ataatcgccg atattttgag gtccgcccgg atctatttta
     2161 ttgatcgccg cctccaggta actgagaatg gtgccgggaa gaataccgcg gatgcctccg
     2221 ccgtctatgg aaagaattcg gatttttttc atggatcagg tgggtttgaa ttcaaagata
     2281 aaaattaaag ggttggccgg tccaaattat tcaccgggtc ccccgaacat gcgggagaag
     2341 gaattttcct tatggtataa ttcaattccc tgatcctccg gtttgggccg aacgatttct
     2401 ttgagaacgg aaggtttgca gtgcataact ttgaaatgat tttccaatgt tgttttttaa
     2461 atttgaaacg aatcgtatga atatatcagt gagcggtaaa tatgcggtgg tttgcggaag
     2521 cacccagggc atcggattgg cggcggccgg caatctcgct gagctcggcg ccaattgcat
     2581 tttaatagcc agaaacgaaa gtgcattggt aagcatcgtt tcagcactgc ccagggttgg
     2641 agaacagcat catcaatacg taacggctga tttttcagat cccaaacagg ttcgccgggc
     2701 aatcgaaagt attgttcgcg ggataacagt ggagatcctc gtaaacaata ccggcggacc
     2761 aaaagcagga ccggtgatgg atgcccttcc ggaggctttt gaagaagcct ttcgggcgca
     2821 tctggtttgt aatcaaatac tggcacaagc cgtgattcct ggaatgaagg cctccggtta
     2881 cggaagaatt atcaatatca tttccacttc ggtaaagaca ccgctgccga atttaggcgt
     2941 gtccaacacg atccgcctcg ctgtcgcatc ctgggcaaaa agcctttcga atgaaatagc
     3001 taaatataat atcacggtaa ataatgtgct tccggggtta acggaaaccg gccggcttca
     3061 gagcctggtc aggcaatcgg cggaggcaga atccaggacg gttgctgaag tggaaggcca
     3121 gatgattcaa agcattccga tgaaacggtt tgggagcccg tcggaaattg ccaatgtcat
     3181 tggctttctc gcttcgccgg cagcctccta tgtgaatggg acgaatatcg ccgtggatgg
     3241 tggaagaacg gcagcacttt aattcgggtt tattgaaatg cggggattgg caggtggccc
     3301 gggcaaccta aaaatactgg aatgaaacgc a
//
LOCUS       DASYCU010000017         5140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3485173, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000017 DASYCU010000000
VERSION     DASYCU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485173"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VGM24_00345"
     CDS             <1..1563
                     /locus_tag="VGM24_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY4153833.1"
                     /translation="AVDAGVGSVMSSFNVVDGIPATGNKWLLTELLRNQWKFKGLVVS
                     DYTSLNEMTAHGMGDLKTVATLAMNAGLDMDMVGEGYLTTLKQSLKEGKVKQAQIDQA
                     CRRILEAKYRLGLFTDPYRRMNTARAEKEILSDAHRKAARKFATHSFVLLKNSDQLLP
                     LKKSGTIALIGPLASNHRNMLGTWSVSGDPEKSVSVMEGIKNVAGDAVKILYAKGANI
                     SDDSTLVKRTNVFGPEIEPETRTPEEMLQEAVETAGKSDVVVAVLGEAADMTGESSSR
                     SDISIPESQENLLKALVKTGKPVVLVLFNGRPLTLEWENSHVPSILDAWFGGTEAGDA
                     IADVLFGNYNPSGKLSMSFPVSVGQIPVYYNARNTGRPWDGKDGPKFKSDYLDIPNEP
                     LYPFGYGLSYTSFSYSGLTPDHPEISPGHPVKIKLTVSNTGNYDGEETAQLYIRDMAA
                     SVSQPVKELKKFQKVFLKKGESRDIEFTLTTRDLQFVGSDLRWVYEPGEFRVFVGGNS
                     RDVVQGELLGKP"
     gene            1583..2200
                     /locus_tag="VGM24_00350"
     CDS             1583..2200
                     /locus_tag="VGM24_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY4153834.1"
                     /translation="MTNISNLIFDLGGVIMDIDVKRTTDAFTGMGVKNISDYFGHGFA
                     ASFFRDHEAGRISDEAFIRKIREGIRLENITDEEVIAAWNALLLCFPPERISLLDQVR
                     KKYRIFLYSNTNGIHYQRFAEMYRNAFPGRELNDHFEKAYYSHVLGHRKPDTTGFEII
                     IRENGLDPAETLFVDDSFMNVEGAIKAGLKGLFLPRGMTINDIRW"
     gene            complement(2274..2537)
                     /locus_tag="VGM24_00355"
     CDS             complement(2274..2537)
                     /locus_tag="VGM24_00355"
                     /inference="COORDINATES: protein motif:HMM:NF027444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HEY4153835.1"
                     /translation="MNIIFLALLIYLLYRFVAGFLIPVFRTTRHVREQFNQRKGGGPE
                     QPGSAGAGEAHSSQDSAPKGSRTNRRPNSSKVGEYIDFEEVKK"
     gene            2698..3189
                     /locus_tag="VGM24_00360"
     CDS             2698..3189
                     /locus_tag="VGM24_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HEY4153836.1"
                     /translation="MDNETQTGRVKEFLAEILAADPALFLVEVRVKPTNNLKVFLDGD
                     QGITIEKCAQVNRALYKKMEESGLFPGGDFSLEVSSAGLDEPLKSLRQYVKNAGRPVE
                     VILLDGSRKEGKLMEVNEEGIVLEESTGKPSPGKAVSKKRELINHTYLFNNIKSTKIQ
                     VVF"
     gene            3211..4458
                     /gene="nusA"
                     /locus_tag="VGM24_00365"
     CDS             3211..4458
                     /gene="nusA"
                     /locus_tag="VGM24_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY4153837.1"
                     /translation="MASINLIEAFQDFKEAENIDRPTMMKVVEDVFKTLLRKKYGSDE
                     NFDVIVNAEKGDLEIMRRRTIVENGTVEDPLAQIAYNEAVKIEPDYEVGEDLYEEIEI
                     LDFGRRAILAAKQTLASRIGDLKKNVLVKKYGERIGEIISAEVYQVWKKEILLLDEEG
                     NELILPKSEQIPQDYFKKGESIRAVVKKVDMKNNSPVIILSRTSPSFLAKLLEIEVPE
                     IFDGLIVIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INWTNNIQLLLQRSLTPSKITSMDIDSEKLHANVYLKPDQVSLAIGRKGVNIKLAQEL
                     TGYSIDVFRDNEGETTEFDIDLDEFSDEIETWVIDELKRIGCDTAHSVLDLTVEELER
                     RTDLEKETIEELRRVLKEEFEKE"
     gene            4562..>5140
                     /locus_tag="VGM24_00370"
     CDS             4562..>5140
                     /locus_tag="VGM24_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY4153838.1"
                     /translation="MAETTTPRLMAAAKEFNIGRDTLIDFLAGKGFSKDELKPTAKLT
                     EDMYRSLQQEFQGDKVAKIKSNQIDLPKGSVEAKRKKEDEAVLFRKETRKPLKEETAA
                     PVEEPRAPTVETPKKPKAEEPKKEPEIIKIEAPDIEGPKVIDKIDLSAIDSSTRPKKT
                     TRKKPTEEPAKEEPRPEEQKAAETPVAGETETD"
BASE COUNT         1488 a         1195 c         1409 g         1048 t
ORIGIN      
        1 gcggtggacg cgggtgttgg cagtgtgatg agttccttca acgtggtcga cggaatcccg
       61 gcaacgggga ataaatggct gctgacagag ctgttgcgca accagtggaa gttcaaagga
      121 ctggtggttt ccgattatac ctctctcaac gagatgaccg cgcacggcat gggcgatctg
      181 aaaaccgttg ccacgctggc aatgaatgcg ggcctcgata tggacatggt gggtgaaggc
      241 tacctgacca cgctgaagca gtccctgaaa gaaggaaaag tgaagcaggc ccagatcgac
      301 caggcctgcc gccggatcct ggaagcaaaa tacaggctgg gcctatttac cgatccttac
      361 cggcgcatga ataccgcacg ggcggagaaa gaaatccttt ccgacgcaca ccggaaagcc
      421 gcccgcaaat tcgcaacaca ttcttttgtc ctgttaaaaa acagtgatca gctgctaccc
      481 ctgaagaaat ccggcaccat tgcgctgatc ggaccgttgg ccagcaacca ccggaatatg
      541 ctgggcacct ggagcgtgtc gggagatccg gagaaatccg tttctgtaat ggaggggata
      601 aaaaatgtag ccggcgatgc cgtgaaaatc ctgtatgcga aaggcgccaa tatttccgac
      661 gatagcacac tggtaaaacg caccaatgta ttcggtcctg aaatagaacc tgaaacccgc
      721 accccggaag aaatgctgca ggaagcggtt gaaacagctg gaaaatccga tgtagtggtg
      781 gccgtgctcg gcgaagcggc ggatatgacg ggcgaatcgt ccagccgctc ggacatctcc
      841 attcccgaaa gtcaggagaa ccttttaaag gcgctggtaa aaacgggcaa gccggtcgtg
      901 ctggtcctct ttaatggcag gcccctgacc ctggaatggg aaaacagtca tgtgccgtcc
      961 attctcgatg cctggttcgg cggcacggaa gccggcgatg ccattgccga cgtgctgttc
     1021 gggaactaca atccctccgg aaaattaagt atgtccttcc ccgttagtgt gggccagatt
     1081 ccggtttatt acaatgcaag gaataccggc cggccctggg acggcaagga tggccccaaa
     1141 ttcaaatccg attaccttga catcccgaat gaaccgctgt atccttttgg ttacgggctg
     1201 agctatacga gcttctccta tagcggtctg accccggatc atccggaaat ttctcccgga
     1261 cacccggtaa agataaaact aacggtaagc aataccggaa attacgacgg cgaggaaacc
     1321 gcacagctct atatccggga tatggcggcc agcgtaagcc agccggtcaa agaactcaaa
     1381 aagttccaaa aggtcttcct caaaaaagga gaaagcaggg atatcgaatt caccctgacc
     1441 acccgggatc tgcagttcgt cggcagcgat ctgcgctggg tgtatgagcc cggagaattc
     1501 cgggtgtttg tgggcgggaa ttcacgggat gtggtacagg gggaactgct ggggaaaccc
     1561 tgaaagcgcg gaataaaaca atatgaccaa tatcagcaac ctcatcttcg atctcggcgg
     1621 cgttatcatg gacatcgatg taaagcggac tacggatgct tttacgggga tgggagtgaa
     1681 aaatatcagt gattacttcg gacacggctt tgcggcctcc ttcttccggg accatgaggc
     1741 gggcaggatc agcgatgagg ccttcattcg gaaaatcagg gaagggatcc ggctggaaaa
     1801 tataacggat gaagaagtga tcgcagcctg gaatgccctc ctgctttgct tcccgcccga
     1861 acggatctcc ctgctcgacc aggtcaggaa aaaataccgg atctttctat acagcaatac
     1921 caacggcatc cactaccagc ggttcgcaga aatgtaccgg aatgctttcc cgggccggga
     1981 gctgaacgat cactttgaga aagcgtacta ttcgcatgtg ctgggccatc gcaagcccga
     2041 cacgacggga tttgagatca tcatccggga gaacgggctt gacccggcgg aaacactctt
     2101 cgtggacgat tcgtttatga acgtggaagg ggctataaaa gccgggctga aaggactatt
     2161 cctgccccgg ggaatgacga tcaacgatat ccgctggtaa tttttggacc tgtaggctct
     2221 tccggcaatc caatgagacg caggcaggcg caataatttt acgaggggag tgactacttc
     2281 ttaacttctt caaaatcaat atactccccg actttggagc tgttgggacg gcggttggtc
     2341 cgggaacctt ttggagcgga atcctgggaa gaatgggctt ctcctgctcc ggccgaaccg
     2401 ggctgttccg gccctccgcc cttcctctga ttgaactgct cgcggacatg gcgggtcgtt
     2461 cggaaaacag ggatcaggaa tccggccaca aagcggtaga gcagataaat aagaagagcc
     2521 aggaatatga tattcatgca gtttcaaaat taaggcagaa ctcgcaaaaa gcctgttaaa
     2581 tagtcaacct ccattttaaa cgcttatttg gatagaatgg tttaaatcca ttaaatttgc
     2641 aaccggtaaa gtgatacaac aggaagggaa cgcaatcgtt cccttcttta ttttcgtatg
     2701 gacaatgaaa cacagactgg ccgggtaaaa gaattcctgg cggagatact ggcggccgat
     2761 ccggctttgt ttttggtgga agtccgggtt aaacccacca ataacctcaa ggtttttttg
     2821 gatggggacc aggggatcac gattgaaaaa tgcgctcagg taaaccgggc cctgtataaa
     2881 aaaatggaag aatccggatt atttcccggg ggtgactttt cactggaggt ctcttcggcc
     2941 gggctggacg aaccgctcaa atcactccgc cagtatgtaa aaaatgcggg ccgcccggta
     3001 gaagtgatcc tgctggatgg cagccggaaa gaaggcaagc tgatggaggt gaatgaagaa
     3061 gggatcgtgc tggaggaaag caccggcaaa ccatctcccg gaaaagcggt ttccaaaaaa
     3121 cgggaactga tcaatcatac ttatttattc aacaatataa aatcaacaaa aatccaggtt
     3181 gttttttaac cggatacaaa aagatctgtt atggcaagta tcaatctgat cgaagcattc
     3241 caggacttca aggaggctga aaatattgac cgtcctacca tgatgaaggt ggtggaagat
     3301 gttttcaaaa ccctgctccg taaaaaatac ggcagtgatg aaaattttga cgtaattgtg
     3361 aatgccgaaa agggagacct tgagatcatg cgccggcgga cgatcgtaga gaatggcacg
     3421 gtggaggatc cccttgccca gattgcttat aacgaagcgg tcaaaattga accggattat
     3481 gaggtggggg aagatctgta tgaagaaata gagatcctcg actttggccg ccgggccatt
     3541 ttagcagcca aacagaccct ggccagccgg atcggcgacc tgaaaaagaa tgtgctggta
     3601 aaaaaatacg gcgagcggat cggggagatc atcagcgcgg aagtttacca ggtatggaag
     3661 aaagagatcc tcctgctgga tgaagaaggc aacgagctca ttcttcccaa atccgaacag
     3721 ataccgcagg actatttcaa gaagggagaa agcatccggg ccgtggtgaa gaaagtggat
     3781 atgaaaaaca attccccggt gatcattctt tccagaacga gtccttcttt cctggccaag
     3841 ctgctcgaaa tagaagtgcc ggaaattttt gatggtttga tcgtgatcaa gaagatcgta
     3901 cgcgagccgg gtgaaagggc aaaagtggcg gtggaatctt acgacgaccg catcgatccg
     3961 gtgggcgcct gtgtcggaat gaagggaagc cgtattcatg ggatcgtacg ggaactgaag
     4021 aatgagaaca tcgatgtgat caactggacc aacaatatcc agctcctgct tcagcgttcc
     4081 ctgactccat ccaaaataac aagtatggac atagacagcg aaaagctgca tgcgaatgtc
     4141 tatttaaagc ccgaccaggt ttcgctggcg atcggaagaa agggcgtaaa tatcaagctg
     4201 gcacaggaac ttaccggtta ttccatagat gtgttccggg ataatgaagg ggagaccacc
     4261 gaattcgata tcgacctgga tgaattcagc gatgaaatcg aaacctgggt cattgacgag
     4321 ctgaaacgga tcggctgcga tacggctcac agcgtgctgg atctcaccgt ggaagaactg
     4381 gaacgcagaa cggatctgga aaaagaaacc attgaagaac tgcgccgggt actgaaagaa
     4441 gaattcgaaa aagaataacc tgtttatcat gcctatatga ttacgggttt tcataataaa
     4501 atatttatca ccgcatgacc aaggtccatg agcggaccag ctgttcatgg atttatttaa
     4561 tatggcagaa acgacaacac ccaggttaat ggcagccgcc aaggaattta atatcggcag
     4621 ggatacgctg atagattttt tggccggcaa gggattcagt aaagacgagc tgaaaccgac
     4681 ggctaagctg acggaagata tgtaccgttc gcttcagcag gaatttcagg gagataaggt
     4741 ggccaaaatc aagagcaacc agattgatct tccgaaaggc agcgtggaag ccaaacgcaa
     4801 gaaggaagat gaagccgtgc tgttccgcaa ggaaaccagg aagccgctaa aggaagaaac
     4861 ggcagctccc gtggaagagc ccagggcgcc aacggtggag acacccaaaa aaccaaaagc
     4921 ggaagaaccc aaaaaagaac ctgaaataat taaaatagag gccccggata ttgaaggccc
     4981 gaaagtgatc gataaaattg atctctccgc aatagattcc tctacccggc caaaaaagac
     5041 aacgcggaaa aaacccacgg aagaaccggc taaagaagaa cccaggccgg aggaacagaa
     5101 agccgcggaa acgcctgttg cgggggaaac cgaaacagat
//
LOCUS       DASYCU010000018         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4789798, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000018 DASYCU010000000
VERSION     DASYCU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4789798"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2364)
                     /locus_tag="VGM24_00375"
     CDS             complement(<1..2364)
                     /locus_tag="VGM24_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4153839.1"
                     /translation="MEFIHSRLQTARPVLPWLAFLILFAFSYQPLQAQQVRISGKVLS
                     SKDQSPLAGVSIRASGTPRGTTTNRDGGFMISAPGAATLYITMQGYLPKELPAGDGNL
                     GTILLDQDLKSLDSVVVVGYGTQKKVNLTGAVATVDMSEKEGHPITNVSNALHGVPGL
                     FVNLGNSQPGVDRSTIRIRGIGTLNENDPLVLVDGIEYSMDELNPEDIASISVLKDAS
                     AAIYGSRAANGVILVTTKTGKGKSHVNYGYYNGIQQSTFLPDAIWDPIAYMKLKDQAE
                     LNEGKQAVDYSDADIAEYQEGMKTDHIVYPASNWFDIALKNGHIQKHDLSVSGSGDKY
                     QYRFSLGYLDRDGIMIGPNNHEKKYSIGVNSSVEVSKRLKVGFTLDGYYRQYTQPFYN
                     SFWSYITRALPILTDTLADGRYGNSWLRTPGRNNWEQPRMIAYTGYGKKTVQRFVSSL
                     YADYKLPFDITYNIKFGVDKYDGILEEFTPQVQTFNPKTFAAINWNSPATAPRAQNTD
                     YNDLNIHFFNTLNWQHSFAASHHVGLMLGSSYDNFGSGSFAAAMTGYLDATLTALDAG
                     TVRYATSGNSTKDVLESYFGRLNYDYNGKYLLEATFRYDGSSRFALGNRWGFFPSVSA
                     GWRIDKENFFKWNDLFSLLKLRGSVGQLGNQAVPLYSYENSIVLGQNYSFDGTLVSGA
                     AATAYSDPNISWETTTTYDAGLDMELLKGRLSFTADLYKKRTSGILRTVNIPDQIGGL
                     AGPQENIGTMDNTGVELTAKYNNHVGAFGYGVSGSLSYNTNKVVDIDG"
BASE COUNT          592 a          676 c          644 g          700 t
ORIGIN      
        1 tccgtcaata tccaccactt tattcgtgtt atacgaaaga ctgccggaaa caccgtatcc
       61 aaacgcgcca acatgattgt tatatttcgc tgtcagttca acaccggtat tgtccatggt
      121 tccgatattt tcctggggac cggccaaacc gccgatctgg tcaggaatat ttaccgtccg
      181 gagaataccg gaagttcttt ttttatacag gtctgcggtg aagctcagtc tcccttttag
      241 caattccata tcgagacccg cgtcgtaggt ggtggtggtt tcccaggaaa tattcggatc
      301 gctgtaggcc gtggcagccg cacccgatac cagggtccca tcgaaactgt agttctgccc
      361 gagcacgatg gaattttcat aactgtacaa gggaacggcc tggtttccca gctggccgac
      421 cgatccccgg agtttaagga ggctgaacaa atcgttccat ttaaagaagt tttctttatc
      481 gatgcgccat ccggctgaaa ccgatggaaa aaatccccac cggttcccca gcgcgaaacg
      541 ggaagatccg tcataacgga aagtggcttc cagcaggtat ttgccgttat aatcataatt
      601 cagacgaccg aaataagact ccagcacatc ttttgttgaa tttcccgatg tggcataccg
      661 gacggttccc gcatccagcg ccgttagcgt tgcatccagg tagccggtca tggcagcggc
      721 aaatgatccg gatccgaaat tatcataact ggaacccagc atcagcccca catgatggga
      781 tgctgcaaaa ctgtgctgcc agttgagtgt attgaaaaaa tggatgttca ggtcattgta
      841 atccgtgttc tgtgcccgcg gcgcggtggc ggggctgttc cagttaatgg cggcgaatgt
      901 tttgggattg aaggtttgca cctgcggcgt gaattcttcg aggatcccgt cgtatttatc
      961 caccccgaat ttgatattat aggtaatatc aaagggtaat ttgtaatcgg catacaggct
     1021 gctcacaaaa cgttgcacgg tctttttccc atagccggtg tatgcgatca tacgtggttg
     1081 ctcccagttg ttccgcccgg gcgttctcaa ccaggaattt ccataccgcc cgtctgccag
     1141 ggtgtccgta agtatcggaa gggcacgcgt aatataactc cagaatgagt tatagaaagg
     1201 ctgcgtgtac tgcctgtaat atccatcgag cgtaaatccg actttcagcc gtttgcttac
     1261 ctccaccgaa ctgttaacgc cgatggaata ttttttttca tggttattgg ggccgatcat
     1321 gatgccgtcg cgatccaggt aacccagcga aaagcggtat tgatatttat ctccgcttcc
     1381 ggaaacactg agatcgtgct tttgaatgtg cccgtttttt agcgcgatat caaaccagtt
     1441 actggcggga taaacaatat ggtctgtttt cattccttcc tggtactctg caatatccgc
     1501 atccgaataa tccacggcct gcttgccttc attcagttcg gcctgatctt tcagtttcat
     1561 gtaggcgatc ggatcccaga tggcatccgg cagaaaggtg ctttgctgta tcccgttata
     1621 ataaccatag tttacatgtg atttgccctt gccggttttt gttgtgacca ggataacccc
     1681 gttcgcggcc cttgatccgt aaatggccgc agatgcatct ttcaacacgg agatgctcgc
     1741 aatatcctcg ggattcagtt cgtccatgga atattcaata ccatccacca ggacaagcgg
     1801 atcattttca ttcagcgttc cgatccccct gatccggatc gtggagcggt ccacccccgg
     1861 ctggctgttt cccaggttca caaacaggcc cgggacaccg tgaagggcgt tgcttacatt
     1921 ggtgatggga tgtccttctt tttcagacat atccaccgta gccacggcac cggtgaggtt
     1981 cacctttttt tgtgtaccgt acccaacgac gaccacggaa tccagggatt tcaggtcctg
     2041 gtccagcaat atcgtgccca ggttgccgtc ccctgccggc aattccttcg gcagatagcc
     2101 ctgcatcgtg atataaagcg ttgcggcgcc gggtgcactg atcataaagc ctccgtcccg
     2161 gtttgtggtt gttccgcgcg gagtgccgga tgcccggata ctcactccgg cgagcgggct
     2221 ttggtctttt gaagaaagca ctttgccgct gatgcggacc tgctgtgcct gtaacggctg
     2281 atacgaaaag gcaaaaagga tcaggaaggc cagccagggc agaacgggcc ttgcagtctg
     2341 caaccgggaa tgaatgaatt ccataatagt cgttttagta gataataatt gatttattta
     2401 aaggatccct gacgggtttt tcatttctgt tttaccatcg attgggatag aagcaaactt
     2461 tttctatagc ggcagatagc catccgccag tttcataaaa tcgatcagtt gttctctttt
     2521 ccagtcattg tcgcggccca gctcttctgc catcaggtcc gctactcttg gcgctgcagc
     2581 tatagccgcc ttcgcgtcga ggaacagcat cc
//
LOCUS       DASYCU010000019         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2761657, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000019 DASYCU010000000
VERSION     DASYCU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2761657"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VGM24_00380"
     CDS             <1..1064
                     /locus_tag="VGM24_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153840.1"
                     /translation="NLARIGDYSAALEYEHKADSTPVSDVENRQIFKSIQALKNIKNA
                     DARRFISFIASNYRVIMLNEAASKPLHRAFAISLLGDLYKRGYRYLAMEMLNPGPDQE
                     LSKLTYQTGQFTSEPVAGEMIRMALEMGFKLVAYEDANAVHHTPRQRDSIQAAHIYQI
                     LQADSSAKIFVYAGYGHIAEKSVSSDFVPMGMAFKKISGIDPLTIDQTDMTEGSNFSY
                     GKAFYEAYLQKYPVSSPSIALSDDEPVNITGSELYDLTVIHPKTVYRDSRPTWLSLQN
                     RRQPVYVKPSNKNTFLVQAYYQYESFNSKPGQVVPADQTYLQSGKGNYLLFLRRGKYI
                     LLFRDWKYKTLYTQHIEVN"
     gene            complement(1057..>1765)
                     /locus_tag="VGM24_00385"
     CDS             complement(1057..>1765)
                     /locus_tag="VGM24_00385"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4153841.1"
                     /translation="VYKEQHQIRQITYPHLFENAATVTDERAGTYAFYPNRDSLAYIP
                     VYGLADAATFIRTTLRHPDFCRGWKDIVAAGLTDDQKIIPSSVKTLADWSIALKPFLN
                     TFNKPLFDQLGLFEDGPLPGGVRCSADILQSLIESKWKMRDQDKDLIVMLHELEYSVH
                     LTEHSLKSSLLVKGENNLRTAMARTVGLPLGIAARLILENKIRLEGLHIPTIPEIYLP
                     VLKELEEQGIRFYESLS"
BASE COUNT          460 a          442 c          419 g          444 t
ORIGIN      
        1 agaatcttgc ccggatcggg gactattccg ctgcactgga atatgaacat aaggcggaca
       61 gcacccctgt ttcggatgtg gaaaaccggc agatcttcaa aagcatccag gcattgaaaa
      121 atataaaaaa tgcagacgcc cgccggttca tcagttttat agcatccaat taccgggtca
      181 tcatgctgaa tgaggctgcc tccaaacccc tgcaccgggc ttttgccata tcgcttctcg
      241 gggatttata taagaggggg taccgctatc tcgcaatgga aatgctgaat cccggaccgg
      301 atcaggagct gagtaaactc acctatcaaa ccgggcagtt caccagtgag ccggtagcag
      361 gtgaaatgat ccgtatggcg ctggaaatgg gttttaaact ggtcgcctac gaagatgcaa
      421 atgccgttca ccatacgccc cgacagagag actccattca ggcggcgcat atctaccaga
      481 tattgcaggc ggacagttcc gcgaagattt ttgtgtatgc tggttacgga catattgcgg
      541 aaaaaagcgt ttcttcagac tttgttccga tgggcatggc atttaaaaaa atatccggca
      601 tcgatccgct caccatcgac cagacggaca tgacggaagg aagcaatttc agctatggaa
      661 aagcttttta tgaagcctat ctacagaagt atccggttag ttcaccctcg atcgcacttt
      721 ccgatgacga accggtaaac ataaccggta gcgaactgta tgatcttacg gtcattcatc
      781 cgaaaaccgt ttaccgcgat tcccgtccca cctggctttc ccttcagaac cggcggcaac
      841 cggtttacgt taagccttcc aataaaaata cctttctcgt gcaggcttat taccagtatg
      901 aatcgtttaa cagcaaaccc ggccaggttg ttccggcgga tcagacctac cttcaatccg
      961 gcaaaggaaa ttatttactg tttctgcgcc ggggtaaata cattcttctc ttccgcgact
     1021 ggaaatataa aacgctctat acccagcata tcgaagtcaa ctaaggcttt cgtaaaaccg
     1081 gatcccctgt tcttccagtt cctttaaaac gggaagatag atttccggga tggtgggaat
     1141 atgcaaacct tccagacgga ttttattttc caggatcagc cgggcggcta taccgagcgg
     1201 aagcccgacg gtcctcgcca tggcagtccg taaattattc tctcctttta ccagcaggct
     1261 gcttttaagc gaatgttccg ttaaatggac cgaatactcc agttcatgta acatcacgat
     1321 caggtcttta tcctgatccc gcattttcca cttcgattca ataagagact ggagtatgtc
     1381 cgccgaacag cggactcctc cgggcaatgg accatcttcg aaaagaccca attgatcaaa
     1441 caggggctta ttaaatgtat tcaggaaagg tttgagggca atgctccagt ccgccaatgt
     1501 cttcacggag gaaggaatta ttttttgatc atcggtcagt ccggctgcca cgatgtcttt
     1561 ccagccccgg caaaaatcgg gatgccggag agtggtccgg ataaaggttg ccgcatccgc
     1621 taatccgtat acggggatgt aggccaggga gtcccggttt ggataaaatg cataggttcc
     1681 ggctctttcg tctgttaccg tagccgcatt ttcaaacagg tgcggatagg taatctggcg
     1741 gatctgatgc tgttctttat atacg
//
LOCUS       DASYCU010000020         5287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3051262, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000020 DASYCU010000000
VERSION     DASYCU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3051262"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VGM24_00390"
     CDS             <1..709
                     /locus_tag="VGM24_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153842.1"
                     /translation="FIQLNPVKAPLNDTSLITRSSVLINSFSYNKMNPKWGFDISNNQ
                     NTSKSLLTYGYQTQTLREWNLRTRWNISRSFLLTSAWKKGNNYLFTSSKNFDNSNYDV
                     NQFSIEPDLSYTQRANLRITLGYRYAEKKNAEIYGGDSYYSGSVNTDAKYNLVSSTSL
                     QAKFTYNDIHYTGNTNSTVSYIMLDGLLPGKNYLWDLDLTKKLGNNLELSIQYEGRKP
                     GEGHTIHTGRASLRAIL"
     gene            complement(740..2689)
                     /locus_tag="VGM24_00395"
     CDS             complement(740..2689)
                     /locus_tag="VGM24_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEY4153843.1"
                     /translation="MKKTALLLAVMAACFQLAAQDQNLLSGRYSRDSLGMILIPLARW
                     EPFPKISDRSGWAKADQTMMKAFVKQAESYIHYDWPSIPATTSLLIERTGDRSEYEAI
                     SFKKREVLGILLLAEIYENKGRFIDPIINGVWSICEESFWGVPAHLPRTKEYSGLMDV
                     SAPFVELFGAETASFLAWVDYFVGDKLDAVSPQIRKRIYYETNNRIFQPLMTKQHGWM
                     ARGADGRPPNNWNPWICSNWLNAALLLEKNEGRRADMVFRLLSVLDQFVNPYPADGGC
                     DEGPGYWDAAAASLYDNLSMLNLASNNAFQYVYQDEKIKNMARFIYRAQIGEKYFLNF
                     ADADPQPGMAAGMIYRFGKDIQDSNMMKFGAFYRKSEEGKTGQFHYFRNFFALFIQDE
                     FRKAPQGLPLPEDVWLPGIQVMIARDKQGSSDGLFLAAKGGNNDESHNHNDVGNFVVY
                     YKGLPVLIDVGRGTYTRKTFSDKRYEIWSNTSAYHNLPTVNGADQPAGPAFKAGQPVY
                     RKDKRSAELSLDITKAYPENAGIQSWRRSVRLNRGKNVELQDIYSFTSAGKITEHFMT
                     CYPAELVKPGELVIHSRPKNETAKDFVISYPADQMQPVVEKVPLTAMEDKGIKEKWGD
                     SIYRINFELNSPGASGRIRFTIAAK"
     gene            2839..3603
                     /locus_tag="VGM24_00400"
     CDS             2839..3603
                     /locus_tag="VGM24_00400"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY4153844.1"
                     /translation="MFSLQNKRAVITGGGSGIGKAIATLFAAQGAEVHIVDLNEANAN
                     AVAEEIRKKGGSSSVQVCDVSDQEQVKKIFNQIQPIDILVNSAGISHVGKLETTTEAD
                     FDKIYRVNVKGVYNCLYAAVLLMKEAKKGVIVNIASIANHVGLADRFAYSMSKGAVYA
                     MTLSVAKDYLSAGIRCNSISPARVHTPFVDGFIAKNYPGKEKEMFEKLSRTQPIGRMA
                     VTMEIAHLVLYLCSDESAFITGTDYPIDGGYITLNS"
     gene            complement(3604..4158)
                     /locus_tag="VGM24_00405"
     CDS             complement(3604..4158)
                     /locus_tag="VGM24_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153845.1"
                     /translation="MKKPVLGAVVGGIIIFLWQFISWAALNLHHASQEYTPKQDSILH
                     YLSTQFSEDGAYLLPNYPKGESREEMEKQMDLNKGKPWAQIQYHRALNVDMGMNIVRG
                     LLADIIMVALICWILLRLSSPGFGRIFTTCLLTGIIVFINSPYTIHIWYPKADIWMHF
                     WDAIVGWSLCGIWLGWLLRRKPAA"
     gene            4377..>5287
                     /locus_tag="VGM24_00410"
     CDS             4377..>5287
                     /locus_tag="VGM24_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4153846.1"
                     /translation="MFKNYFRITVRNLLRNKAFSVINIFGLAIGISTCLIILLFVLNE
                     FSYDRFNKKADQMVRVVFRGFVQGEKMKEANVMPPVAQALKADYPEVLDATRLRDYGR
                     PRFITGEKSFAEEPFAYVDSNFFQVFTFPLLQGNPKTALMQTHSIVISELVAKKYFGR
                     ENPMGKMMNFKGWDAPYKITGVMKDMPDNAQFHFDLLGSMTSLPESREQSWMTSNYYT
                     FLVLPKGYDYKKLEAKMPQVVEKYMGPQLQASMGMSLAEFRKKGNDLGLYLQPLTDVH
                     LHSDLMFELSTVGDLQQVCILGAIAVF"
BASE COUNT         1337 a         1315 c         1304 g         1331 t
ORIGIN      
        1 atttatccag ctgaacccgg tcaaagcgcc tttaaacgac acctccctga tcacgcggtc
       61 ctccgtgctg atcaattctt tttcctataa taaaatgaat ccaaaatggg gatttgatat
      121 cagcaataac cagaatacct ccaagtcgct gctcacctat ggataccaga cccagacatt
      181 gagggaatgg aacctgcgga cgcgctggaa tatttcccgg tctttcctgc tcacctcggc
      241 ctggaaaaaa ggaaacaact acctgttcac cagcagcaag aattttgaca acagcaatta
      301 cgacgtgaat cagttttcca ttgaaccgga cctgagctat acgcagcgcg ccaatctccg
      361 gatcaccctg ggctaccgct atgcggagaa aaaaaatgca gaaatatatg gcggggacag
      421 ctactattcc ggttcggtca ataccgatgc gaaatacaac ctggtatcga gcacctccct
      481 gcaggctaaa ttcacataca acgatattca ttataccgga aataccaaca gcacggtgag
      541 ttatatcatg ctggacggac ttctgcccgg caagaattat ctctgggacc tcgacctcac
      601 caaaaaactc ggcaataacc tggagctaag catccagtat gaaggacgca aacccggtga
      661 agggcatacc attcacaccg gccgcgcttc cctgcgggcc atcctctaag gggaaaataa
      721 aacgatcttt gcttattagc tattttgccg cgatcgtgaa acggatcctg ccgctggccc
      781 cgggggagtt cagttcaaaa tttatccggt aaatggaatc gccccatttc tctttgattc
      841 ctttatcttc catggcggtg agaggaactt tttccacgac cggctgcatc tggtccgccg
      901 gatagctgat cacaaaatcc tttgcggttt cgtttttcgg cctggaatga atgaccagtt
      961 ctccgggctt taccagttcg gccggataac aggtcatgaa gtgttccgta atcttgccgg
     1021 cggatgtaaa ggaataaata tcctgcagct ccacattttt cccgcggtta aggcggacgg
     1081 accggcgcca gctttggatg cccgcatttt ccgggtaggc ctttgtgatg tccagcgaaa
     1141 gttcggccga ccttttatcc ttgcggtaaa cgggctggcc ggctttaaag gccgggcccg
     1201 ccggctgatc ggccccgtta acagtcggca gattatgata ggccgaggtg ttggaccaga
     1261 tttcatatcg tttgtcgctg aatgttttcc gggtataggt ccctcttccc acatcgatca
     1321 gtaccggcag ccctttataa taaaccacaa aattgcctac gtcattgtga ttatggcttt
     1381 catcgttatt tccgcctttg gcggccagga ataatccgtc ggaacttccc tgtttatccc
     1441 ttgcgatcat cacctggatg ccgggcagcc acacatcttc cggcagggga agaccttgcg
     1501 gcgctttccg gaattcatcc tggatgaaga gagcaaaaaa gtttctgaaa taatggaact
     1561 ggccggtctt tccttcctcc gatttgcggt aaaaggcgcc gaatttcatc atattgctgt
     1621 cctgaatatc ttttccaaag cgatagatca tgccggccgc catacccggc tgcggatccg
     1681 catcggcaaa attcaaaaag tatttttcgc cgatctgcgc ccggtaaata aaacgcgcca
     1741 tgttttttat tttctcatcc tggtaaacat attggaacgc attgttgctg gcgaggttga
     1801 gcatggaaag gttatcatat aaagaggcag cggcggcgtc ccagtacccc ggaccttcat
     1861 cgcaaccgcc atcggccgga tagggattca caaactggtc cagaacggat aagagccgga
     1921 ataccatatc ggcgcgcctt ccttcattct tttccagcag cagcgcggca ttcagccagt
     1981 tcgaacagat ccagggattc cagttattgg gcggacggcc atccgcaccc cgggccatcc
     2041 agccgtgctg ctttgtcatc agcggttgaa agatccggtt gttggtttca taatagatcc
     2101 gcttccggat ctgcggcgat acggcatcca gtttatcgcc gacaaaataa tcgacccagg
     2161 caagaaagct ggcggtctcc gcaccgaaca gctccacaaa aggcgctgac acatccatga
     2221 gaccggaata ttcttttgtc cgcggaagat gcgccggaac accccagaag gattcttcgc
     2281 aaatggacca gaccccgttg atgatcggat cgatgaacct tcccttgttc tcgtaaattt
     2341 ccgccagcag caggatgccc agcacttctc tttttttaaa actgatcgct tcatattcgc
     2401 tccggtctcc ggtgcgctcg atcaagaggg atgtggtggc gggaatagaa ggccagtcgt
     2461 aatggatata ggattccgcc tgctttacga aagccttcat catggtttga tccgctttcg
     2521 cccagccgct tcggtcgctg atctttggaa agggctccca ccgggccagt ggaattaaga
     2581 tcattcccag gctgtccctg gaatatcttc cgcttagcag gttttgatcc tgcgccgcca
     2641 gttggaagca ggcggccatt acggcaagca gcaatgcggt tttcttcata atgctctttc
     2701 gttatccctt cgaaaataat ctaaaaatgc cttttcaaaa tattttcggg attatttgcc
     2761 cgttatgcgc ggcgcaggcc aaagcagtta tgtcttatat ttaacccgga aaatattttt
     2821 aaaatttaat tattccacat gtttagtctt cagaataagc gggcggtcat taccggcggc
     2881 ggcagcggga tcgggaaagc gatcgccacg ctgtttgcgg cgcagggagc agaagtccat
     2941 attgtcgacc tgaatgaagc aaatgcaaat gcggtggcgg aggagatccg gaaaaaaggg
     3001 ggcagttcct ccgtacaggt ctgcgatgta tcggatcagg agcaggtaaa aaagatcttt
     3061 aaccaaattc aaccgatcga catcctggtg aacagtgcgg gcatttccca tgtgggaaag
     3121 ctggaaacca ccacggaggc tgactttgac aaaatttacc gggtgaatgt gaaaggggtg
     3181 tacaattgtc tttatgcagc cgtactcctc atgaaggagg caaaaaaagg cgtcatcgtc
     3241 aatattgcct ccatcgccaa tcatgtgggg ctggccgacc gttttgccta ttccatgagc
     3301 aagggcgcag tatatgccat gaccctttcc gttgcgaagg attatttatc cgccggcatc
     3361 cgctgcaatt ccatatctcc cgcgcgtgtg cacacgccgt ttgtagatgg ctttatcgcc
     3421 aaaaactatc ccgggaaaga gaaggagatg tttgaaaaat tatccaggac ccagcccatc
     3481 ggccggatgg ctgtaacaat ggagatcgcg cacctcgttc tttatttatg cagcgatgaa
     3541 tctgctttta ttacgggaac ggattatccg atcgacgggg gatatatcac cctgaacagt
     3601 tgatcaggca gctggttttc ttcttagcag ccatcccagc cagatcccgc agagcgacca
     3661 gccgacaatg gcatcccaga aatgcatcca gatatctgct tttggatacc agatgtgaat
     3721 ggtatagggt gagtttatga atacgatgat cccggtaagc agacaggtag tgaagatcct
     3781 gccgaagccc ggggaggaaa gccggagcaa tatccagcag atcagggcga ccatgataat
     3841 atcggccagt aagccccgca cgatattcat tcccatgtct acgttgagcg cccggtgata
     3901 ctggatctgg gcccagggtt ttcctttgtt gagatccatc tgcttttcca tttcttcccg
     3961 ggattcccct ttgggataat tgggcagcag ataagcgccg tcttcggaga actgggtgct
     4021 gagataatgg aggatgctat cctgtttggg cgtatattcc tggctggcat ggtgcaggtt
     4081 gagtgcagcc caggaaataa attgccataa aaaaatgatt attccgccaa cgacggcgcc
     4141 aaggaccggc tttttcataa cggttatttt aggttaaaaa atcgggacgt ttaagttaag
     4201 gaatatccgg atgcaaaaac gctttttaaa aacggacacc cactatatca ttaacggaca
     4261 ctgttccggg agagccctta ttcaagcacc tgattatcaa cgatattcac aaatggcatt
     4321 tttttggaac ggttgcggcc gggtcattga gaagataaaa ctgaaaacca gatcgaatgt
     4381 ttaaaaatta tttccgcatc accgtccgca atctcctgcg caataaggct ttctccgtca
     4441 tcaatatttt tggattggcg atcggcattt ccacctgcct gatcatttta ctttttgtat
     4501 tgaatgaatt cagctacgac cggttcaata aaaaagcaga tcagatggtc cgggtcgtgt
     4561 tccggggttt tgtgcagggc gaaaaaatga aggaagcgaa tgtgatgccg cccgtagcgc
     4621 aggccctcaa agcggattat cccgaagtgc tggacgcgac ccggctccgg gattacggaa
     4681 ggccccgctt tattaccggg gaaaaatctt ttgcagagga accctttgcc tatgtagact
     4741 ccaatttctt tcaggtcttt acattccctc ttttgcaggg gaacccgaaa acggcgctga
     4801 tgcaaacaca ttccattgtg atctctgaat tggtggcgaa aaaatatttt ggccgggaaa
     4861 atccaatggg caagatgatg aatttcaaag gttgggatgc gccctataaa ataaccggcg
     4921 tgatgaagga tatgccggac aatgcacaat tccatttcga tctgctgggc tccatgacca
     4981 gtctgccgga atcccgggaa caatcctgga tgacctccaa ctattatact ttcctggtgc
     5041 ttcccaaagg atatgattat aaaaaattgg aggcaaaaat gccgcaggtg gtggaaaaat
     5101 atatgggacc gcagctgcag gcttcgatgg gcatgagcct ggccgaattc cggaaaaagg
     5161 ggaatgatct cgggttatac cttcaacccc tgaccgatgt tcatctgcat tcggatttaa
     5221 tgtttgagtt atctacggtg ggggatttac agcaggtgtg cattttagga gccattgcag
     5281 ttttcat
//
LOCUS       DASYCU010000021         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5080016, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000021 DASYCU010000000
VERSION     DASYCU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5080016"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VGM24_00415"
     CDS             complement(<1..361)
                     /locus_tag="VGM24_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY4153847.1"
                     /translation="MGETWNKKEREKKRLQNRKEKAEKKLERKENSKDGNDLDSMLAY
                     LDENGNLTSKPPDPRKKISINAEDIHVSVPKQEPLNPEDLIRKGTVTFFNDSKGYGFI
                     KDKETQESVFVHINSLTE"
     gene            760..2118
                     /locus_tag="VGM24_00420"
     CDS             760..2118
                     /locus_tag="VGM24_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY4153848.1"
                     /translation="MNFKAIVFSAFPSFIFFHSAIYAQQPANQKERMRIADTMENSLK
                     KELLNVWYPRAVDSLDGGFLSTFTYDFKPTGNQDKMIVTQARHVWVNAKASVRYPSVA
                     YYKTGARHGFEFLKNKMWDKTDGGFYALVDRKGDPVQGTDKQAYGNAFAIFALSAYYK
                     SSGDTNALNLAKKGFLWLEKHSHDPVYKGYFQHMKRDGTPIKRTADIPSTAETGYKDQ
                     NSSIHLLEALTELYGVWKNDLVRERLNEMLLLIRDKMVSPRGDLVLFFQPDWQPVSYS
                     DSGREIIMAHRNLDHASFGHDVETAYLLLEASRALGIRDESETRRVGKKMVDHALRNG
                     WDSAIGGFYDEGYYFKGDPGITIIKDSKNWWAQAEGLNTLLLMADLYTDDPMHYFDKF
                     KLLWKYTRIYLIDHEHGDWYEEGLDKEPERKTALKGHIWKAAYHNYRALTNCIDRLRT
                     TN"
     gene            complement(2108..3199)
                     /locus_tag="VGM24_00425"
     CDS             complement(2108..3199)
                     /locus_tag="VGM24_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY4153849.1"
                     /translation="MRNIFLPVFVFLLSQAALPGNAQTASAGGNNKGLKDYYRDYFPV
                     GVAVGPRNLSGAEADLILREFNSITPENAMKMGPIHPREDQYNWKNADSILNFAGRHG
                     LKVRGHNLCWHEQEPDWLFKDKDGKQVSKEVLLKRLKDHITAVVGRYKGRIYAWDVLN
                     EAIDDDSTKFLRNSLWYQICGDEFIAKAFQYAHEADPGAILFYNDYNATRPEKRERIY
                     KLLKRLVDAKVPINAVGIQGHWSLTEPTEKNLRDAIERFSSLGLDVQVTEMDISIYPW
                     EKNPRPKRETDLDVFTPELQQRQTNQYRMIFRVLRDYRKQITGVTFWNISDKETWLDR
                     YPVNGRKNYPLLFDQDLKRKKAYWEVVNW"
     gene            3355..>3855
                     /locus_tag="VGM24_00430"
     CDS             3355..>3855
                     /locus_tag="VGM24_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4153850.1"
                     /translation="MPNSAPALRRTLNSTMLWGLGVGYVISGMYFGWNLGLEKGGTLG
                     LGIATLFIIVMYLAFTFSYAELACSIPRAGGAFDYASRALGKDLGFLAGMAQNIEFIF
                     APPAIAFAIGAYFNLFFPQLPILTVAIFVYLLFTGLNIYGVKAAASFELLITILAVAE
                     LLLFSGI"
BASE COUNT          936 a          920 c          915 g         1084 t
ORIGIN      
        1 cttctgtcag ggaattgata tgtacaaaaa cactttcctg tgtttctttg tctttgatga
       61 atccgtatcc ttttgaatca ttgaaaaaag taacggttcc tttccggatc agatcttcgg
      121 gatttaaggg ttcctgctta ggcacactga catggatatc ctctgcattg atgctgatct
      181 ttttccttgg atccggaggc ttggaagtga ggtttccatt ctcatccagg taggccagca
      241 tgctgtccag gtcgttgccg tccttactgt tttccttacg ttccagtttt ttctcggcct
      301 tctctttcct gttctgcagt ctctttttct ctctttcctt cttgttccat gtttctccca
      361 tactactgaa taatttaatt ttaaatgtgt tattaaataa atacagatcc ggctaattaa
      421 ataccggtcc ggctaaaata acgaaaataa ttctacgaaa tgaaccgcgt attttaagcc
      481 tgaccccgcc agatccgatt aaagatacgt gaatattttt cttcttccca gccgggtttt
      541 acaataccct gaaaccctta tccacagtgc acttaaacga attaagacgg atgccggata
      601 acgagaaccg gggtatggtc agtggtgctt agccgatcct ggttttgatc ccgccaggct
      661 cttttggctg cccctgttgc ctggctgacg aaggattttt tttgccggat acggggcccc
      721 tgacccgaat tcaataaatt tatacttcaa cccgggatca tgaatttcaa agccatcgtt
      781 ttttccgcat ttcccagctt tatttttttt cattccgcta tatatgccca gcagccggcc
      841 aatcagaagg agcggatgag gatcgcggat accatggaaa attccttaaa gaaagaattg
      901 ctgaacgtgt ggtatccgcg cgccgtggat agcctggatg gcggcttcct gagcaccttc
      961 acgtacgatt ttaaacctac cggcaaccag gataaaatga tcgttaccca ggcaaggcat
     1021 gtatgggtga atgcgaaagc atcggtgcgt tatccttcgg tggcttatta taaaaccggc
     1081 gcccgtcacg gatttgaatt tctcaaaaat aaaatgtggg ataaaacgga tggtggtttc
     1141 tatgccctgg tggaccgcaa aggagacccg gtgcaaggga ccgataagca ggcttacggc
     1201 aatgcatttg cgatctttgc cctgtctgcc tattataaat cttcggggga taccaatgcg
     1261 ctgaaccttg caaagaaggg atttctatgg ttggaaaaac atagtcatga tccggtttac
     1321 aaaggctatt tccagcatat gaaaagagat ggcacgccca taaaaagaac ggccgatatc
     1381 ccttccacgg cggaaaccgg atataaagac caaaacagtt cgatccacct gctggaagcc
     1441 ctcaccgaat tatacggagt ctggaaaaac gacctggtgc gggagcgcct gaatgaaatg
     1501 ttattgctga tccgggataa gatggtttcg ccccggggcg acctggtgct tttctttcag
     1561 ccggactggc agccggtgtc ctacagcgat tccggaaggg aaatcattat ggcacaccgt
     1621 aacctggatc atgcttcctt cgggcacgat gtggaaacgg cctatcttct gctcgaagct
     1681 tcccgcgcat tgggcatccg ggatgaatcc gaaacccggc gggtcgggaa aaaaatggtg
     1741 gatcatgcgt taagaaatgg ctgggacagc gccataggcg gattttatga tgaaggatat
     1801 tattttaaag gagatccggg aatcaccata atcaaagaca gcaaaaactg gtgggcccag
     1861 gcggaaggat tgaatactct tttactcatg gcagacctgt atacggatga tcccatgcat
     1921 tattttgata aattcaaatt gctctggaaa tatacccgga tttatttaat cgatcacgaa
     1981 cacggggact ggtatgaaga aggattggat aaagaacccg aacggaagac cgcactgaaa
     2041 ggtcatatct ggaaagcggc ctatcacaat taccgggcgc tcacgaattg tatagaccgg
     2101 ctgaggacta ccaattaacc acttcccaat aggctttttt gcgtttcagg tcctgatcga
     2161 acaataaggg atagtttttt ctgccattta ccggataccg gtccagccag gtttccttat
     2221 cggaaatatt ccagaacgta actccggtga tctgcttccg gtaatcccgc aatacccgga
     2281 atatcatccg gtactggttg gtctgccttt gctgaagttc cggcgtaaag acatccagat
     2341 ccgtttcccg tttgggacgc ggattttttt cccagggata aatggaaata tccatttcgg
     2401 tcacctgcac gtctaaaccc agggaagaga accgttcgat agcatctctt aaattctttt
     2461 ccgtgggttc ggtaagcgac cagtgaccct ggatgcccac tgcatttatg ggtactttgg
     2521 catccaccag ccgttttaat aatttataga tacgctctct tttttcagga cgcgttgcat
     2581 tatagtcgtt gtaaaagagg atggcgccgg gatccgcttc gtgcgcatac tgaaatgctt
     2641 tggcaataaa ttcgtcgcca cagatctggt accaaaggga gttccggaga aattttgttg
     2701 aatcatcgtc aatggcttcg ttcagcacgt cccaggcata aatgcgtccc ttgtagcggc
     2761 caaccacggc cgtaatatgg tctttcaacc gtttcagcag gacttccttg cttacctgtt
     2821 ttccgtcctt gtctttaaac agccagtccg gctcctgttc gtgccagcat aaattgtgtc
     2881 ctctcacttt cagtccgtgc cgcccggcaa agttcagaat ggagtcggca tttttccagt
     2941 tgtactgatc ttcccgggga tggatcgggc ccatcttcat cgcattttcg ggcgttatac
     3001 tgttgaattc ccgcaggatc agatctgcct ctgctcccga aagattgcgt gggccgacgg
     3061 ccacgccgac cgggaaataa tcccggtaat aatccttcag tcctttgtta tttccgcctg
     3121 ccgaagccgt ttgcgcattt ccgggtagag cagcctggct tagaagaaag acaaaaaccg
     3181 gtaagaaaat atttcgcata aaaatatttt taaaggaatg gaaagaatcc cctgcctgtt
     3241 cttaaaaacc ccctgaaaaa taatctattt tcaaataaat acatgggata aaccgcggta
     3301 tccgccgtgg tgcaggtaac ggtaattaat actatattgt gagtaaacat ttccttgcca
     3361 aattccgctc ctgcattaag gcgaacgctc aactccacca tgctctgggg gctgggtgtc
     3421 gggtacgtta tttccgggat gtattttggg tggaacctgg gactggaaaa aggaggtacg
     3481 ctgggtttgg gcattgccac gctgttcatc atcgtcatgt acctggcctt cacgttcagc
     3541 tacgccgagc tggcctgttc cattcccagg gccgggggag cttttgatta tgccagccgg
     3601 gcattgggaa aggaccttgg ttttctggct ggaatggcgc agaatattga attcatcttt
     3661 gctcccccgg cgatcgcatt tgcgatcggg gcctatttca atttgttttt tccccaattg
     3721 ccgatcctca ccgtggcgat ttttgtttac ctgcttttca ccggtctgaa tatttatgga
     3781 gtgaaagctg ccgcttcctt tgaactgctg atcacgattc ttgccgtggc tgagctgctg
     3841 ctgttttcgg gcatc
//
LOCUS       DASYCU010000022         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3051726, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000022 DASYCU010000000
VERSION     DASYCU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3051726"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..713)
                     /locus_tag="VGM24_00435"
     CDS             complement(72..713)
                     /locus_tag="VGM24_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY4153851.1"
                     /translation="MKKIISFSLVAFFFLVNAGFKVSKTEGWVSLFDGKTLNNWKVGE
                     NAATFTVENGMIVAHGKTAHLFYVGDVKNHNFKNFDFKADVMTRPGSNSGIYFHTQYQ
                     EGGWPSKGYEVQVNNSHSDWRRTGSLYGIEDVKEVWVKDNVWFTEHITVQGKRVIIKI
                     NDRTVVDYTEPANVQRPKDMEGRIISSGTFALQGHDPNSQVYFKNLMVKPLDD"
     gene            complement(731..1621)
                     /locus_tag="VGM24_00440"
     CDS             complement(731..1621)
                     /locus_tag="VGM24_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY4153852.1"
                     /translation="MRTTYTRVLYMSIFFSGVLSTGYSQPDNGKLRVIMFGAHPDDCE
                     KASGTAALFAQMGYAVKFVSVTNGDAGHQVMKGEELAKRRYAEAQEAGRRLGVSYDVL
                     NNHDGLLMPSLDVRMQIIREIREWNADIVFAPRPNDYHPDHRYTGVLVQDAAYMVAVP
                     NILPETPALKKNPVFLYYQDRFQRPNPFRPDITVDISSVYEKKLAAFDAHVSQVYEWL
                     PWVGHYLEEVPQDKNERIQWLGKRYLERVKPETLAVLTKWYGKERAEKAQYVESFEIC
                     EYGMQPQTDDIKKLFPMLGK"
     gene            complement(1717..>2460)
                     /locus_tag="VGM24_00445"
     CDS             complement(1717..>2460)
                     /locus_tag="VGM24_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153853.1"
                     /translation="SISPDGKTIYLPSLEGDDWKVVDAKTGNVLHRVVLHSGSHNTIY
                     GPDGKKVYLEGLKSNYLTVVNTADQKVISATGPFVNHVRPFTINARQSRCFVNADGLL
                     GFEIGNLQTGKKIAHIEVKGFHTGPVKRHGCPSHGIALTPDEKEIWLADGFNEAIHIF
                     EINKDKVSQAGTIKLSDQPGWITFSVDGKYAMPSTGDIIDVRGKKIIATLRDETGAPV
                     QSEKVVEVQFGGKKLLKAGNQFGIGRVTR"
BASE COUNT          576 a          653 c          525 g          706 t
ORIGIN      
        1 tttattttaa ggaagtggct gtaagacccc tgcacaaaaa atagccgtcc tttctttacg
       61 gccagccggc atcaatcatc cagcggtttc accatcaggt ttttaaagta gacctggctg
      121 ttgggatcat gcccctgcag ggcaaacgtt ccgctggaaa tgattcttcc ttccatgtct
      181 ttgggacgct gaacattggc cggttcggta tagtctacga cggtcctgtc atttatttta
      241 atgatgaccc tttttccctg cacggtaatg tgctccgtga accatacatt atccttaacc
      301 cacacctcct tcacatcctc tatcccatac aggctgccgg tccggcgcca gtcggaatgg
      361 gagttgttca cctgtacttc atatcctttg gaaggccagc ctccttcctg atattgggta
      421 tgaaaataaa ttcccgaatt ggatccgggc cttgtcatta catctgcttt gaaatcaaaa
      481 tttttaaaat tgtgattttt aacatccccg acataaaaca aatgggcggt tttaccgtgc
      541 gccacaatca ttccgttctc cacggtaaac gtggccgcat tttcacccac tttccagtta
      601 ttaagcgttt tgccgtcgaa cagcgatacc catccttccg ttttactcac cttaaatccc
      661 gcgtttacca ggaagaagaa ggctaccagg gaaaaagaaa taattttttt cataaatgat
      721 cttgtttgtt ttatttgccc agcatgggaa aaagcttttt tatatcgtcg gtctgcggtt
      781 gcataccata ttcacatatc tcaaaggatt cgacatactg cgctttctcc gcccgttctt
      841 ttccatacca tttcgtcaga acagccaatg tttccggctt aacccgttcc aggtatcttt
      901 tgccgagcca ttggatccgt tcgtttttat cctgagggac ttcttccaga taatgaccta
      961 cccagggaag ccattcatat acctgcgaca catgcgcatc gaatgccgcc agctttttct
     1021 cataaacgga actgatatcc acggtaatgt ccgggcggaa gggattcggg cgctgaaacc
     1081 ggtcctggta atataaaaag acggggttct ttttcagagc gggggtttcc ggcagaatat
     1141 tgggcaccgc caccatatag gccgcatcct gcacgagcac gcccgtatac cggtggtccg
     1201 ggtgataatc gttgggacgc ggagcaaaaa cgatatctgc attccactcc cggatctccc
     1261 ggatgatttg catacgcaca tccagggaag gcatcagcaa accatcgtga ttgttcaaca
     1321 catcatagct tacccccagc cgtcttcctg cttcctgtgc ttccgcatat cttcttttgg
     1381 caagctcttc tcctttcatc acctgatgac cggcatcccc attggtcacg gatacaaatt
     1441 ttactgcgta gcccatctgt gcaaaaaggg ccgcggtccc ggacgctttt tcgcaatcat
     1501 cgggatgggc gccaaacatg atcacccgga gcttgccgtt atcgggttgc gaataaccgg
     1561 tagacagcac gccggaaaag aaaatgctca tatataaaac ccgggtatag gttgttctca
     1621 taaacaggaa tttggggagt gatcggggaa gcgtaattcg gattatgaag ataatgttta
     1681 tggacgatat aaacttttcc cgcgatttcc ggtggtttac cgggtaactc tcccaatgcc
     1741 aaactggttg cctgccttca gcagcttttt ccccccaaat tgcacttcga ccactttttc
     1801 actctgcacc ggcgctcccg tttcatcccg gagtgtcgca ataatctttt ttcccctcac
     1861 gtcgataatg tcgccggtgg aaggcatcgc atatttccca tcgacgctga atgtaatcca
     1921 tcccggctga tcgcttaatt ttatggtacc cgcctggctg accttatcct tgtttatttc
     1981 aaaaatatgg atcgcttcat taaaaccatc tgcaagccag atttccttct catcgggtgt
     2041 cagggcaatg ccatggcttg ggcatccatg cctttttacc gggcccgtgt gaaacccttt
     2101 cacttcgata tgggcgattt tcttccccgt ttgcagattc ccgatttcaa atcccagcag
     2161 gccatctgca ttcacaaaac aacggctctg tctggcattg atggtaaagg gcctgacatg
     2221 attgacgaac ggacctgttg ctgaaataac tttctgatcc gcggtgttta ccacggttaa
     2281 atagttcgat ttcaatcctt cgagatatac cttcttccca tcaggtccat aaattgtatt
     2341 gtgggatccg gaatgaagca ccacgcgatg aagaacattt cccgtttttg catccactac
     2401 tttccagtcg tccccttcga gggaaggcag gtaaattgtc tttccatccg gtgatatgga
//
LOCUS       DASYCU010000023         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_297755, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000023 DASYCU010000000
VERSION     DASYCU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297755"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VGM24_00450"
     CDS             complement(<1..1092)
                     /locus_tag="VGM24_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY4153854.1"
                     /translation="MRAWHILIILLTCSGTIYAQQDSIAERVHLPNGWSLTPVGSSLP
                     LGDLPLNIAVSPTKKLIAVTNNGQSTQTIQLIDAVRFRVLSQVVIPKSWLGLVFSGDG
                     KSLYASGGNDNRILKYNIVQGKLILSDSISLGKPWPVKISPAGMELDNASRKLYVVTK
                     ENNSLYTVDLRTGKSNSLALGAEAYTCLLSADRKELYISLWGGDKILVYNTVQKKIIR
                     RIAVGDHPNDICLTRNGKFLFVANANDNSVSVVDIKKGEVVETLNAALYPESRTGSTT
                     NGVALSQDDKTLYIANADNNCLAVFDVSRPGSCKSRGFIPVGWYPTAVKVIGNRVFVA
                     NGKGFTSLPNPRYRAFDTEKTLAYQKGAKK"
     gene            1241..1867
                     /gene="eda"
                     /locus_tag="VGM24_00455"
     CDS             1241..1867
                     /gene="eda"
                     /locus_tag="VGM24_00455"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY4153855.1"
                     /translation="MLSSFLTKQILPAVTLQKEEDALPVAEAMMKGGLEIMEITFRTD
                     IAARAIRRISTEYPEMIVGAGTILNTSQLQMAIDSGARFALSPAFNAPVCTEAKRLDF
                     PFIPGIMTPTEMEAAYGLDYRILKLFPSEQIGGIAFLKSLSGPYGHLGIQFIPMGGVT
                     LQNMDGFLKIPGVIAIGGSWLASQELIAEKNYPAITENVRRALQKVNN"
     gene            1937..>2671
                     /locus_tag="VGM24_00460"
     CDS             1937..>2671
                     /locus_tag="VGM24_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4153856.1"
                     /translation="MKKDAKPTRIRYSILAVIFINVVINYMDRSNISVAAPFILKYFS
                     LDTVRMGYIFSAFGWTYAFLQIPGGILTDRFGPRILYSISLILWSFASLLQGFAGGFA
                     KLLGLRIAIGAFEAPAYPMNNRLVTTWFPDGERASAIAIYTSGQFIGLAFLTPVLLTL
                     EHFLGWQGLFYCTGGIGLVWGIVWWFIYRDPLQHPRVNRAELEHIEKGGGLIHQNEKS
                     HTKFEWKNLRAVLSNRKLWGIYIGQFG"
BASE COUNT          653 a          683 c          635 g          700 t
ORIGIN      
        1 ctttttagct cctttctgat atgccagcgt tttctctgta tcaaatgccc ggtaacgggg
       61 attgggcagg gaggtaaacc ctttcccgtt cgcaacgaaa acgcggttgc cgattacctt
      121 aacggctgta ggataccagc ccaccgggat gaatcccctc gatttacagg atccgggacg
      181 actgacatca aataccgcca ggcaattatt atctgcattc gcgatgtaca gggtcttatc
      241 atcctgactg agcgctacgc cgttggttgt tgagccggta cgggattccg gatacagcgc
      301 tgcatttaag gtctccacta cttccccttt ctttatatcg accacagaaa cgctgttgtc
      361 atttgcattg gcaacaaaaa ggaatttccc gttcctggtc aggcagatat cattcggatg
      421 gtcccccacg gcaatccggc ggatgatctt tttctgtacg gtgttataaa ctaaaatttt
      481 atccccgccc cagagagaaa tataaagttc ctttctgtcc gccgacaaca ggcaggtata
      541 ggcttccgcc cccaacgcca gggaattgga tttccctgtt ctcagatcga cggtatacag
      601 actgttattt tctttggtaa ccacatacag cttccgcgag gcattatcca actccattcc
      661 ggcaggagaa attttcaccg gccagggctt cccgagtgaa atgctatccg acaaaatcaa
      721 ttttccctga acaatattgt atttcagaat acggttatcg ttgccgccgg acgcatacaa
      781 tgattttccg tccccgctaa aaactagccc cagccagctt tttggaatga ccacctggct
      841 caggaccctg aatcgtacgg catcaatcag ctgaatggtc tgggtgcttt gcccgttgtt
      901 agtaaccgct atgagttttt tggttggcga cacggcaata ttcagcggga gatctccaag
      961 aggcagagaa cttccgaccg gcgtcaggct ccatccgttc ggcagatgga cccgctccgc
     1021 aatggaatcc tgttgcgcat atatggttcc tgaacaggtc agcagtataa ttaatatatg
     1081 ccatgctctc atgattctca aatttgggaa ctccggcgcc tttacgaaac tatacaaaaa
     1141 taggtagcag gcattaatta aatgatattt tttaacgctc cggtatccct taccatttgt
     1201 cacggaataa aaatatattt gacaggcaca aaaaccaaag atgctatcct ctttcctgac
     1261 caaacagatt ttgccggcgg taacccttca aaaagaagaa gatgcgctgc ccgtggctga
     1321 agccatgatg aaaggcggcc tggaaatcat ggaaatcacg ttcagaacgg atattgcagc
     1381 ccgcgctatc cggcggatca gcactgaata tccggaaatg atcgtcggtg ccggcacgat
     1441 cctcaacact tcccaattac agatggcgat cgattccgga gcccgttttg cattgagccc
     1501 cgctttcaat gccccggttt gcacagaagc aaagcggctc gactttcctt ttattcccgg
     1561 gatcatgaca cccacggaaa tggaagccgc ttacggactg gattaccgga tcctgaaact
     1621 ttttccttcg gaacagattg gcgggattgc ctttcttaaa agtctttccg gcccatacgg
     1681 tcatctgggc attcagttta ttcccatggg cggcgtaacc cttcagaaca tggacggatt
     1741 tttgaagatc cccggagtga ttgccatcgg cggctcctgg ctggcctctc aagagctcat
     1801 tgcagagaaa aattatccgg ccattacgga aaatgtgcgg cgcgcgctac aaaaagtaaa
     1861 caactgaccc gggtttcctt tcgctgcggg aaggaaaacc ggatatagaa cgattgaata
     1921 aactttccag gggcttatga aaaaagatgc aaaaccgacc cggatccggt attcaatcct
     1981 ggccgtcatc tttatcaatg tggtgatcaa ttatatggac cgcagtaata tttcggtggc
     2041 ggctcctttt atcctcaaat acttttcact ggatacagta agaatgggat atatattttc
     2101 agcttttggc tggacttatg cctttctcca gattcccggt ggaatcctga ccgatcgttt
     2161 cggtccgagg atcctgtaca gcatcagtct gatattatgg tcgttcgcca gcctactgca
     2221 gggctttgcc ggcggctttg ccaagctgct gggattacgg attgccattg gggcttttga
     2281 agcgcccgcc tatccgatga ataaccggct ggtcaccacc tggtttccgg acggggaaag
     2341 agcttccgcc attgcgatat atacttccgg gcagtttatc ggacttgctt ttttaacgcc
     2401 cgtcctgctg accctggaac atttcctggg ctggcagggt cttttttact gcaccggcgg
     2461 gatcgggctg gtctggggaa ttgtctggtg gtttatttac cgcgacccgt tgcagcatcc
     2521 ccgcgtaaat cgggccgaac tggaacacat tgaaaaaggc ggcggcctga ttcatcaaaa
     2581 tgaaaagagc cacacaaaat ttgagtggaa aaatctccgg gccgttcttt ccaatagaaa
     2641 actctgggga atatatatcg gccagttcgg t
//
LOCUS       DASYCU010000024         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_442752, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000024 DASYCU010000000
VERSION     DASYCU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442752"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VGM24_00465"
     CDS             complement(<1..453)
                     /locus_tag="VGM24_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY4153857.1"
                     /translation="MKKLDWYILRKFLSTFVFTMLTITVIAVVIDTSEKADDFVKSGL
                     SSWEIVTHYFVGFVPFIMAMIFPLMVFIACIYFSSKMAGQSEFVAILAGGVRYPRMLR
                     PYLVGALILGLIYWLASRYWVPKANEIRTDFQATYIDRNSTYNSDPYRT"
     gene            590..2539
                     /locus_tag="VGM24_00470"
     CDS             590..2539
                     /locus_tag="VGM24_00470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY4153858.1"
                     /translation="MSAKRWLLMTAMIFLVSINSFSQDTVNYDQHEAFAPLFYPAYGD
                     QVRTAGGTPGPEYWQNEADYSIQANLDDENQKINGTVAITYKNNSPEQLSFVWLQLDQ
                     NTYRPDSRAQATTSVNGGRYANLDFNGGYDIKSVFILDGEREEKARYSISDTRMQILL
                     SEPLKAHGDSIKIRIEYSFGIPERGSDRMGRMQTDNGWVYEIAQWYPRMCVYDNVQGW
                     NTLPYLGAGEFYLEYGNYDFSITASSKFVVVASGELLNAAEVLNAEERKRLETARNSD
                     KTVIIRSEKEVKAESAKPGKGRQTWHFHCQRARDVAWAASAAFVWDAARINLPDGKKA
                     LAMSVYPVEYALDSGWKRSTEFVKGAIEHYSQKWLPFPYPNAVNVAGPVGGMEYPGIV
                     FCSAFSQKKDLWGVTSHEFGHTWFPMIVGSNERKYAWMDEGFNTFINSVADKYFNHGE
                     FYEAPRYRERALRFFKPNAESIMTIPEVVQPGYLAADAYDKPAMGLQLLREQVLGEGK
                     FDSAFRYYIRSWAYKHPTPWDFFHAIENYSGETLDWFWRGWFFNNWKLDQAVMNVEYL
                     ESNPVNGAIITIENREQLPMPATVEIKETGGKTGRVKLPVEIWQRGPVWKFFYPSIRK
                     ISSVTIDPDKDLPDNDESNNVWKSN"
     gene            complement(2540..3679)
                     /gene="tgt"
                     /locus_tag="VGM24_00475"
     CDS             complement(2540..3679)
                     /gene="tgt"
                     /locus_tag="VGM24_00475"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY4153859.1"
                     /translation="MPQLTFELVKTDSSSRGRAGLIRTDHGEIPTPIFMPVGTAGSVK
                     AVLSDQLEQEIKAKIILGNTYHLYLRPGTPVLESAGGLHRFMNWEKPILTDSGGYQVF
                     SLAGTRRITEEGVLFKSHIDGSPHLFTPEKVMDIQRSIGGDIIMAFDECPAFGSGYDY
                     AKSSMELTHRWLDRCLERFGREPDKYGYSQSLFPIVQGGIYEDLRKQSCEFISSRNAA
                     GNAIGGLSVGEPDEQLYEMTELCCSHLPEQRPRYLMGVGTPWNLLECIALGVDMFDCV
                     MPTRNGRNGMLFTSKGVINIDNKKWENDFSPLDDGIACRFSNQYSKAYLRHLMKSREL
                     AALTIASVHNLSFYLHLVSQAREHILLGDFCSWKNQQTELLKQRL"
     gene            3826..>4260
                     /locus_tag="VGM24_00480"
     CDS             3826..>4260
                     /locus_tag="VGM24_00480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4153860.1"
                     /translation="MITGIQLFYYAWFFQRLAFFRKPLKIKSQQHPVSVIICARDEAP
                     NLAANLPGVLVQTYTSTHEVIVVNHNSQDDTRYLLEEFKKTFRDLHIVNLEYEAKGIP
                     GKKYPLSMGIKEARHEVVLLTDTDCVPASECWIEKMQDGYDEG"
BASE COUNT         1110 a         1042 c         1095 g         1013 t
ORIGIN      
        1 cgtccggtag ggatcgctgt tataggtgct gttccggtcg atataggtgg cctggaaatc
       61 cgtccggatt tcattggctt taggcaccca gtaccggctc gccagccaat agatcagccc
      121 caggatcagg gcccccacca ggtaaggacg cagcatgcgg ggataccgca ccccaccggc
      181 caaaatggca acaaactcgg attgcccggc cattttcgaa ctgaaataga tacaggcaat
      241 gaaaaccatc agcgggaaga tcatcgccat gataaagggc acaaaaccga caaaataatg
      301 cgtgacaatc tcccaggaac tgagtcctga ttttacaaaa tcatcggctt tttcgctggt
      361 atcgatcacc acggcgatca cggtgatcgt aagcatggta aacacaaagg tgctcaggaa
      421 cttgcggagt atgtaccaat ccagtttttt catgatcaga aaagcattgc gaaaatacag
      481 gaatgcggtc tggtaaacga aaccggcttg ttaaatccag gaccttctat catattccgg
      541 acgcaaaaaa ccctgaaaaa tttaccttcg ctcccttaaa atggtaaaga tgtccgcaaa
      601 aagatggtta ctgatgacag ccatgatatt tctggtctcg ataaatagtt tttcccagga
      661 taccgtaaac tacgaccagc acgaagcgtt tgcaccgctc ttttatcccg cttatggcga
      721 ccaggtcaga acggccggcg gaacaccggg cccggaatac tggcagaacg aggcggatta
      781 ttccattcag gcaaacctcg acgatgagaa tcaaaagatc aacggcacgg tggccataac
      841 ctataaaaat aacagcccgg agcaattgtc ctttgtctgg ttacaactgg atcagaatac
      901 gtaccggccc gattcccgtg cgcaggccac tacttcggtg aatggcggac ggtatgcaaa
      961 cctggacttt aacggggggt atgatatcaa atctgttttt atcctggacg gagaaaggga
     1021 agaaaaagca aggtacagca taagcgatac gcgcatgcag atccttttat ccgaaccatt
     1081 aaaagcgcac ggagattcca taaagatccg gatcgaatac agtttcggta tccccgagcg
     1141 cgggtcggac cggatgggca ggatgcaaac cgataacgga tgggtttatg aaattgccca
     1201 atggtacccg cggatgtgtg tgtacgataa tgttcaggga tggaataccc tgccctacct
     1261 cggggccggt gaattctacc tggaatacgg caactatgat ttcagcatca cggcttcatc
     1321 aaaatttgtg gtagtggcat ccggcgaatt actcaatgcg gcggaggtgc tcaatgcgga
     1381 agagcgtaaa agactggaga ccgcccggaa cagcgataaa acggttatta tccgcagtga
     1441 aaaagaagta aaagcggaat ctgccaaacc gggaaaaggc aggcagacct ggcatttcca
     1501 ttgccagcgg gcgcgggatg ttgcctgggc cgcctccgcc gcttttgtgt gggatgccgc
     1561 aaggatcaac ctgcccgatg gaaaaaaagc gcttgccatg tccgtttacc cggttgaata
     1621 tgcattggat tccgggtgga agcgttctac cgaatttgtg aaaggcgcca ttgaacatta
     1681 ttcccagaaa tggctgcctt tcccttatcc caatgccgtg aatgtggccg gtcccgttgg
     1741 cggcatggag tatccgggca tcgtattctg cagtgcgttc agtcagaaaa aagatctgtg
     1801 gggagtgacc agccacgaat tcggtcatac ctggttcccg atgatcgtag gcagcaatga
     1861 aagaaaatac gcctggatgg atgaaggctt taataccttc attaattcgg tcgctgataa
     1921 gtatttcaat cacggagagt tttatgaagc gccccgctac cgggaaaggg cattgcggtt
     1981 ttttaagccg aacgcggaga gcattatgac cattccggag gttgtccagc ccggttacct
     2041 ggccgcggat gcttatgata aaccggccat ggggctgcag ttattgcggg aacaggtttt
     2101 aggcgaagga aaattcgaca gtgcctttcg ctattatatc cggagctggg cgtataaaca
     2161 tcccacgccc tgggattttt ttcatgccat agaaaactac agcggggaaa ccctggactg
     2221 gttctggaga ggctggttct tcaacaactg gaagctggat caggcggtca tgaatgtgga
     2281 gtacctggaa tccaacccgg taaacggtgc gatcatcacg atcgagaaca gggaacaact
     2341 gcccatgccg gcaacggttg aaataaagga gaccggaggt aaaaccggcc gggtcaaact
     2401 gccggttgaa atctggcagc ggggtccggt ctggaaattt ttttatcctt ccatcaggaa
     2461 aatttcttct gtcacgattg atccggataa agatctgccg gacaatgacg aaagcaacaa
     2521 tgtctggaaa tccaattgat cacaaccgtt gtttcagcaa ttccgtctgt tgatttttcc
     2581 agctgcaaaa atctccgagc aggatatgtt cccgggcctg gctgaccagg tgcagataga
     2641 aggaaagatt atgcacgctg gcgatggtta gcgcagcaag ttccctgctt ttcatgaggt
     2701 gccggagata ggctttgctg tattgattgc tgaaacggca ggctatgcca tcatccagcg
     2761 gcgaaaaatc attttcccat tttttattgt cgatattgat cacccctttc gaagtaaaaa
     2821 gcattccatt ccttccgttg cgggtaggca ttacacaatc gaacatatcc actcccagtg
     2881 cgatgcactc cagcagattc cagggcgtgc ccacgcccat gaggtaccgg ggtctttgct
     2941 ccggcagatg ggagcagcag agttccgtca tttcatacag ctgttcatcc ggttctccca
     3001 cacttaagcc gccgatggca tttcctgccg cattccggga cgaaatgaat tcgcaggatt
     3061 gtttgcgcag gtcttcgtaa atgcctccct gcacgatcgg aaacaggctc tgcgaatacc
     3121 cgtatttgtc cggctccctg ccgaaccgtt ccaggcaccg gtccagccag cgatgggtaa
     3181 gctccataga ggatttggca taatcgtatc cggaaccgaa agccgggcat tcatcaaaag
     3241 ccatgataat atccccgccg atgctccgct gaatatccat tactttttcc ggggtgaata
     3301 aatgaggcga gccatcgata tgagatttga ataagactcc ttcttccgtg atcctgcggg
     3361 ttccggcgag agaaaagacc tgatatccgc cgctgtctgt taaaatgggt ttttcccagt
     3421 tcatgaaacg gtgcaggcct ccggcacttt caagcaccgg agtcccggga cgcaggtaaa
     3481 gatggtacgt gttgcctaaa atgatcttcg cctttatttc ctgctccagc tggtctgata
     3541 aaaccgcttt tacgctcccg gccgttccta cgggcataaa aatgggcgtt ggaatttctc
     3601 cgtgatccgt gcggataagt cctgcgcggc cccgggagga agaatctgtt ttgacaagtt
     3661 cgaacgttaa ctggggcata tgataaacgc gcaagttaag gttgaattac tacccgcgta
     3721 acccctatat tgtgtaaatt tgctttcccc cactaaccgc actactgaat gaaccatttg
     3781 ccggcttttt tatcgtggaa tgagatttgg tttttctttt tttgtttgat caccgggatt
     3841 cagcttttct actatgcctg gtttttccaa agactcgctt tcttccggaa acccttaaaa
     3901 ataaaatccc agcaacatcc cgtcagtgtt atcatctgcg cgcgggatga agcgcccaat
     3961 ctcgccgcca atcttcccgg cgtgctggtg cagacctata ccagcaccca tgaagtgatt
     4021 gtggtgaatc ataacagcca ggatgatacc cggtacctgc tggaggaatt caaaaagacc
     4081 ttccgggatt tacatattgt gaacctcgaa tatgaagcca aggggattcc cgggaaaaaa
     4141 tatccgctca gcatggggat caaggaagcc cggcatgaag tggtattgct tacggatacc
     4201 gattgcgttc cggccagcga atgctggatt gaaaaaatgc aggatggtta tgacgagggt
//
LOCUS       DASYCU010000025         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4935748, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000025 DASYCU010000000
VERSION     DASYCU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4935748"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VGM24_00485"
     CDS             <1..793
                     /locus_tag="VGM24_00485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4153861.1"
                     /translation="KYLPRISPGNEAFGETYTDRARFIGRYFREQYALLKSREEQPAY
                     FREQLIYNYLYKGPVLEWYAKIKTRLEKNYQFFHELLPRQGHISDIGCGYGFMSYQLH
                     FAAPGRDITGFDYDEDKIAVANHCFSRDERIRFEAADVSLMQPEAADGFILADVLHYL
                     KPEQQLILLEKCMDKLNPGGVLLLRDGDRDLETRHGGTKLTEYFSTRVFAFNKTVHKQ
                     LFFLSGKAIRDLASRKNMNCRSIDPSKYTSNIIFVITHPGQYEKI"
     gene            780..>1589
                     /locus_tag="VGM24_00490"
     CDS             780..>1589
                     /locus_tag="VGM24_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY4153862.1"
                     /translation="MKRFDVLIIGSGMGGLVCGNILSREGYSVCLVEKNKQFGGCLQI
                     YVRDKVVFDAGVHYLGGLDKGQNLYQIFKYLGLMDKLKLERMDEAFDRILLDDDEKLY
                     EIAQGYDAFIQRLVRDFPEEENAIRTYCETVKEICDRFPMYRLRMEGEGKEKQEVMGI
                     SAKKFIDQLTNNEKLRAVLAGNNMLYAGAGEQTPLYVHALIVNSYMESAWRCTNGGSQ
                     ITKTLTANIHAAGGVLLRNREVVKIQVGDGRATHVLLKDGTHIEAKEFISNI"
BASE COUNT          453 a          341 c          418 g          377 t
ORIGIN      
        1 aaaatatctt ccccggattt ctccgggcaa tgaagccttt ggtgaaacct atacggatcg
       61 cgccagattc atcggaagat atttccggga acaatatgcc ttattgaaaa gcagagaaga
      121 gcaacctgcc tatttccgtg aacaactcat ttacaattat ctctataagg gcccggtact
      181 ggaatggtat gcaaaaatca agacccggct ggagaaaaat taccagtttt ttcatgagct
      241 tttaccgagg cagggacata tatccgatat cgggtgcggg tatggtttca tgagttacca
      301 actccatttt gctgctccgg gcagggatat taccggattt gactatgatg aagataagat
      361 tgccgtggcc aatcactgtt ttagtcggga tgagcggatc cgttttgaag cggcagacgt
      421 ttccctaatg caaccggagg ccgctgacgg ttttatcctg gccgatgtgc ttcattatct
      481 gaaaccggaa caacagttga tcctgcttga aaaatgcatg gacaaactga acccgggagg
      541 cgtattgctg ctgagggatg gggaccgaga cctggaaaca aggcacggag gaacaaaact
      601 taccgaatat ttctccaccc gcgtttttgc ctttaacaag accgtgcata agcaattgtt
      661 ctttctgtcc ggtaaggcaa tccgggacct ggcttcgcgt aaaaacatga attgccgctc
      721 catcgatccg tcgaaataca catcaaacat catttttgtc atcactcatc ccgggcaata
      781 tgaaaagatt tgacgtattg atcatcggca gcggcatggg cgggcttgtt tgcggaaaca
      841 tcctcagcag ggaaggatac agtgtctgcc tcgtggaaaa gaacaagcag tttggaggct
      901 gtctgcagat ctatgtgcgg gacaaagtcg tttttgatgc aggcgtgcat tatctcggcg
      961 gactggacaa gggtcagaac ctttaccaga tctttaaata cctgggactg atggacaaat
     1021 tgaaactgga gagaatggac gaagcttttg accggatcct gttggatgat gatgagaaac
     1081 tttatgaaat agcccaggga tatgatgcat tcattcagcg gctggtccgg gattttcccg
     1141 aagaggaaaa tgccatccgg acatactgtg aaacagtaaa agagatctgt gaccggttcc
     1201 cgatgtaccg gctccggatg gaaggggagg gaaaggaaaa acaggaagtg atggggattt
     1261 cagcaaaaaa attcattgac cagctgacga acaatgaaaa gttacgggca gtcctggccg
     1321 gaaacaatat gttatacgcc ggagcgggag agcaaacgcc cctatatgtg catgcgctga
     1381 ttgtaaacag ctatatggaa agtgcctgga gatgtacaaa tggcggatca cagatcacca
     1441 agacccttac ggccaatatt catgccgccg gaggggtcct tctcagaaac cgggaggtgg
     1501 ttaaaatcca ggtcggggac ggccgcgcaa cgcatgtcct gttaaaagac ggaacccata
     1561 ttgaagcaaa ggaatttatt tcgaatatt
//
LOCUS       DASYCU010000026         6177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4791946, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000026 DASYCU010000000
VERSION     DASYCU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4791946"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VGM24_00495"
     CDS             complement(<1..944)
                     /locus_tag="VGM24_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HEY4153863.1"
                     /translation="MNFCIRVSAGGVISRGKQLGACLGIFVACCLSMTSSAQVAVAFP
                     SKTGDSTNTVYIWHSDTLREVQKDSVAIQSLFGHVKLQSEKTIFYCDSLAINHKENFI
                     EAFGKVHINDNDSTDIYSQYMKYFVDTKFIIFKKNVLLKDGRGVLSTENLTYDMNQHI
                     GNYLDGGKVVNQKTTVTSREGTYYADTKDVYFKKDVVLKDPAYDLTADSLLYNSEQQL
                     ATFITSTFIRDSSGRTIRTSEGFYDLKNHRAQFGKRPTIRDGSQTITAEDIHFDDSLG
                     ISTATGNAVFRDTAEGLAVISNSMIADKKKNTLLATQKP"
     gene            complement(944..1939)
                     /locus_tag="VGM24_00500"
     CDS             complement(944..1939)
                     /locus_tag="VGM24_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY4153864.1"
                     /translation="MKISNETKIGALTAIAIALLILGFNFLKGKDLFEHSKKIYAVFK
                     GVEGLETSNAVKIKGLEVGSVYTISPVDKNVDGILVTISMKKDVNIPKNSVAVIDAGL
                     IGSASIILKKGDATDYIRDGDTLITQEKGNLLSEVQGNLSPVVSRLSSTLESLDSLIR
                     IIGGLFDPNLKNNLGAIIANLARSSASLEVLLNEKTGALAHTMVHLDSFTNNLANNNQ
                     RISGTLDNLEKTTNKLANAKIDETLQSAREAMTSLKDAVNKMNSRDGSLGLLLNDKKL
                     YQNLESTIRSLNTLLDDLRMHPKRYVNISVFGKKDKSGPLTAPLSDSTAKPPNQN"
     gene            complement(2055..3044)
                     /locus_tag="VGM24_00505"
     CDS             complement(2055..3044)
                     /locus_tag="VGM24_00505"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY4153865.1"
                     /translation="MLRSVSGFIFSIALLFSFSSFRGGELPGGRSAKIRTLIIDPGHG
                     GLYPGCRGLISTEKDVTLDVSLKLGEAIRKNFPNIKVVFTRTTDACVDNATNLHDDLH
                     ARAQIANQAKGDLFISIHCNSTRQPAGGYYEKRVIGHKKKLQYVGRGKKRRKRWVNAP
                     IYKSYWVKNMRVGTETYIWKADRNEKKGDAINERDEGGEDVTDSSTDESSFDLTSPEA
                     RMRAQLYEKKFFDNSALFASLVEQSFASAGRQSYGVKQRDVGIQVLQATGMPSVLIEI
                     GFLSNKEEEEYLNSNSGQDEVVQNIVDALKRYKDQLEGSPETIQAVPSGSDNQ"
     gene            3221..5830
                     /locus_tag="VGM24_00510"
     CDS             3221..5830
                     /locus_tag="VGM24_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEY4153866.1"
                     /translation="MPPSNKIPVPSNDTIPQKIVAAARDTIPADSSQADSLPFRSDSA
                     SLKTDTVHFPVSKDSLDAPVSYSAADSVVLDVPTKNVILYNKANTKYKDINLDAYQIR
                     MDQPRNLLTATYILDTANRIIGKPKMTQAGSTMQSDSMVFNMKTQKGITVNTFTQSGE
                     MFVMGEKMKKISKDEYYAFHGRFTTCNLDTPHFAFRTNKMKLINKKMAISGPVHPEFE
                     GVPIPIYIPFGFFPISQGRHSGFLPPTFNVSSQYGLGLEGIGYYKVLSDNFDVQFRAN
                     LYSYGGYNLYFTPEYRVRYRYSGRLNFVYQRTRTLSNSGKTPYDDSKTYSLNWSHVVD
                     SKAHPGQTFSANLNLMSTKFNRYVLNDPTANFNNQISSSIAYSKTWNGKYNLTVSGNH
                     SQNNQTGLVNVNVPSIGFTAVTIYPFAPKEFVGTQKWYQKLGIGLNTSISGLTSFYDS
                     LFSIHHMLDTFQYGAQNNIPISLSLPPLGPLQISPGVSYQNRILSKKFDFNWDEARQK
                     VDTSFNKGIYVANSLSFSMNFSTAIFGTFQGFGKKSNILGIRHTIRPSFGFSYSPDLA
                     KTYYKNIQVDKFGHYQNLNLFTGNVYPGFSPGTFGGINFGIDNNLEMKLKSKTDTSSG
                     ANQKVKLIDGFGFTGSYNYLADSFKLSNFNIYFRSTLFQKINISASTNLDPYQYDTLG
                     FRLDKYTWQGKGGFNPGHITGGNIAISTSFKSKPKDEKKAAEEKKGEDSQLPMTLEEQ
                     QAELNYIRNNPAEFVDFNIAWSVNISYSLNFSRVLRPDYSGYKTQLTSSLMLNGDFNL
                     TEKWKVSYSTYYDVKNLKIQSFTGSLSRDMHCWQLSINVTPVGLYRSFSITINPKSGI
                     LRDLRINRNRTFN"
     gene            complement(5823..>6177)
                     /locus_tag="VGM24_00515"
     CDS             complement(5823..>6177)
                     /locus_tag="VGM24_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4153867.1"
                     /translation="NEKSRRDSFIKMHEVLNMGLHMCIYPEGTRNTGTEPLKSFHDGA
                     FRLAIGSGKSIIPMVIFNSRRANPPLKGFFLLPVKLYLDFLPEVKSPPGETVEQLKNR
                     VHDLMKQYILSHSPN"
BASE COUNT         1472 a         1523 c         1489 g         1693 t
ORIGIN      
        1 ggtttctggg tggccagcag ggtatttttt ttcttgtcgg cgatcatgct gtttgaaatc
       61 acggcaagcc cttcggccgt atcccggaat acggcgtttc ccgtagcggt acttataccg
      121 aggctgtcgt caaaatgaat atcctctgcc gtaatggttt gtgatccgtc ccggatggtc
      181 ggccttttcc cgaactgcgc ccggtgattc ttcaggtcgt aaaatccttc gctggtccgg
      241 atggtgcgtc cgctgctgtc ccggataaat gtggaggtga tgaaggtggc gagctgctgc
      301 tcactgttat ataacaggga gtctgccgtg aggtcatagg ccggatcctt cagcaccacg
      361 tctttcttaa aataaacatc cttggtatcg gcgtaatagg tgccctccct gctggttacg
      421 gtggtcttct ggttcactac ttttccgcca tccaggtaat tgccgatatg ctggttcatg
      481 tcgtaggtca ggttctccgt gctgaggact ccccggccgt ccttcagcaa tacatttttc
      541 ttaaaaataa tgaatttggt atccacgaaa tatttcatgt actgggaata gatatcggtg
      601 gaatcattat cgttgatatg aaccttcccg aaggcttcga tgaaattttc cttatggttt
      661 atcgcgaggc tgtcgcaata aaaaatggtt ttttcacttt gcagctttac gtggccgaac
      721 aggctctgaa tggcaacgga gtctttctgc acttcgcgca gggtatctga atgccagata
      781 tagaccgtgt tggtactgtc gccggttttt gaaggaaagg caacggccac ctgggcggag
      841 gaggtcatac tcaaacagca ggcaacgaaa attcccaggc atgctcccag ttgttttccc
      901 cgtgaaatca ctccgccggc tgatacccgg atgcaaaagt tcatcaattc tgatttggcg
      961 gtttggccgt ggaatcgctg agcggggcag ttaacggtcc gcttttatcc ttttttccaa
     1021 aaacggaaat gttcacatac cgtttcgggt gcatgcggag atcgtccagc agggtattca
     1081 ggctgcgtat ggtggattcc aggttctgat ataatttttt atcattcagc aataaaccga
     1141 gtgagccatc cctgctgttc attttattga cggcatcctt gagggaggtc atggcttccc
     1201 gggcgctttg cagggtttcg tcaatttttg cattggccag tttattggtt gtcttttcca
     1261 ggttatccag ggtgccgctg atccgctggt tattgttcgc caggttgttg gtgaaactgt
     1321 ccagatgaac catcgtatgg gcaagggcgc ccgtcttttc attcagaagc acctccaggg
     1381 acgcagagga acgcgccagg ttggcaatga tggcgcccag attattcttc aggttgggat
     1441 caaaaaggcc gccgatgatc cggatcagcg aatcgaggga ctccagcgta ctgctcagcc
     1501 gggaaacgac cgggctgagg ttcccctgca cttcggataa gaggttgcct ttttcctggg
     1561 tgatcagggt atcgccgtcc cggatatagt ccgtggcatc tccttttttc agaatgatgc
     1621 tggccgaccc gatcaggccg gcatcgatca ccgcaaccga attctttgga atattcacgt
     1681 ctttcttcat gctgatggtt accaggatgc catccacatt cttatccacc ggactgatgg
     1741 tatatacgga gcccacttcc agccccttga tctttaccgc atttgaggtt tccaatcctt
     1801 ccactccctt gaatacggca tagatcttct tgctgtgttc gaagaggtcc tttcccttta
     1861 aaaaattaaa tccaagaatc aggagggcga tggcaatggc cgtgagtgcg cctattttcg
     1921 tttcatttga gattttcatt ccgggatctt ttttgagaaa caccttgcta aagcttcttt
     1981 cctgcttgtt tataagctta caaacctaca ggaaaaagat cattgggcaa agatggatcg
     2041 ggaggcccgc ggccctattg gttgtccgaa ccggagggaa ccgcctgaat ggtttccggc
     2101 gaaccttcca gttgatcctt atatcgtttc agggcgtcaa cgatattctg cacgacttca
     2161 tcctgcccgc tattgctgtt caggtattct tcttcttcct tattgctcag gaatccgatc
     2221 tctatcaaga cgctgggcat gccggtggcc tggagcacct ggatacccac atccctttgc
     2281 tttacgccgt aactctgccg gccggcagag gcaaaagatt gctctacgag cgaggcaaaa
     2341 agtgctgaat tatcaaagaa tttcttttcg tagagctggg cgcgcatgcg ggcctccgga
     2401 gaggtcaggt caaaagacga ttcgtcggta ctgctgtcgg tgacatcttc gccgccttcg
     2461 tcccgttcat tgatggcatc cccttttttc tcgttacggt cggctttcca gatataggtt
     2521 tccgtgccca cccgcatgtt ttttacccag taggatttat atatgggtgc attgacccat
     2581 ctttttcttc tcttttttcc gcggcccaca tactgtagct tttttttatg gccgataact
     2641 cttttttcat agtatccccc cgccggctga cgggtggaat tacaatggat gctgatgaag
     2701 aggtcgcctt tggcctggtt ggcgatctgg gcgcgggcat gcaggtcatc gtgtaaattg
     2761 gtcgcgttat caacacaggc gtctgttgtc cgcgtaaaca ccactttgat attgggaaaa
     2821 ttttttctga tggcttcgcc cagtttaagg gaaacatcca gcgtaacgtc cttttcggta
     2881 gaaatgagac cccggcatcc cgggtaaagc cctccgtgtc ccggatcgat aatcagggtg
     2941 cggatcttgg ccgatcttcc tccgggcagt tcgccgcccc ggaaggacga gaatgaaaat
     3001 aaaagggcaa tggaaaaaat gaatcccgaa accgatctca gcatactttt ctttttgaca
     3061 gtcaagatgt taatcttcac aatttcttag acaggatact cctatttttg cagcattaca
     3121 ttgtatgaat aacggaggca aaattagttt aaaatacgtt tccacgttga tttttgcacc
     3181 ggtattcctt tgtataactg ttaacagttc aggaagttat ttgcctccgt ccaataaaat
     3241 tccagttcct tcaaacgata cgatcccaca aaaaattgtt gccgccgcga gagatactat
     3301 ccctgcggat tccagccagg cagacagcct tccgttccgg tccgattcgg catccctgaa
     3361 aacggatacg gttcatttcc ccgtttccaa ggattcgctg gatgcgccgg tctcctacag
     3421 tgcggccgac tccgtcgtgc tggacgtgcc gaccaagaat gtcatccttt acaataaggc
     3481 caataccaag tataaggata tcaacctgga cgcctaccag atccggatgg accagccccg
     3541 gaacctcctc acggccacct atatactgga tacggcaaac cggatcatcg ggaaaccgaa
     3601 gatgacccag gcgggaagta ccatgcaatc cgattccatg gttttcaata tgaaaaccca
     3661 gaaagggatc acggtaaata cgtttaccca aagcggggaa atgtttgtga tgggggagaa
     3721 gatgaagaaa atttccaagg atgaatatta tgccttccac gggcgcttca ccacctgcaa
     3781 cctggatacg ccgcattttg cgttccggac caataagatg aagctcatca acaaaaagat
     3841 ggcgatctcc gggccggtac acccggagtt cgagggcgtg cccataccga tctatattcc
     3901 gtttggattc ttcccgatct cgcagggcag gcattccggc tttttgccgc ccacgttcaa
     3961 cgtgagttcc cagtatggcc tgggtctgga agggataggt tattataagg tgctcagtga
     4021 taattttgat gtccagttca gggcaaatct ctattcatac gggggatata atctgtattt
     4081 cacgccggaa taccgggtcc ggtaccggta tagcggccgg ttgaatttcg tttatcaaag
     4141 aacgcggacc ctgagtaatt cgggaaaaac gccctacgac gattcaaaga cctatagtct
     4201 caactggagc catgtggtgg acagcaaagc ccatccgggg cagacctttt cagcgaacct
     4261 caatctgatg tccaccaagt tcaaccgcta tgtcttaaac gatcctacgg cgaattttaa
     4321 caaccagatc agttcttcca ttgcctattc taaaacctgg aacggtaaat ataacctgac
     4381 ggtaagcggt aaccacagcc agaataacca gaccgggctg gtgaatgtga atgtgccttc
     4441 cattggtttt acggcggtca cgatttatcc ttttgccccg aaagagtttg tgggcaccca
     4501 gaaatggtac cagaaactgg ggatcggcct caataccagc atttccggcc tgaccagttt
     4561 ttatgattcg cttttcagca ttcatcatat gctggatact ttccagtatg gagcgcaaaa
     4621 caatattccg atctcgctgt ctcttcctcc gctcggtccc ctgcagattt cccccggcgt
     4681 gagctatcag aaccggatcc tgtctaaaaa attcgatttt aactgggatg aagcccggca
     4741 gaaagtggat accagcttca ataagggtat ttatgtagcc aattcccttt ccttctccat
     4801 gaatttcagc acggccattt tcggaacctt tcagggcttt ggtaaaaaaa gcaatatcct
     4861 ggggatcagg cacacgatcc ggcccagttt cgggttttct tattcgccgg acctggcaaa
     4921 aacctattac aagaacatcc aggtggataa gtttgggcac tatcagaacc tgaatctttt
     4981 taccgggaac gtgtatccgg gtttttctcc gggaactttt gggggcatca atttcggaat
     5041 agacaataac ctcgagatga agttaaaatc caaaacggat acctcttccg gagccaatca
     5101 aaaggtgaag ctgatcgatg gtttcggatt taccggaagt tataattacc tggcggactc
     5161 ttttaagctc tccaacttta atatttattt cagaagcacc ctgtttcaga agatcaatat
     5221 ttcggcttcc accaaccttg acccctatca gtatgatacc ctgggtttcc ggctggataa
     5281 atatacctgg cagggcaagg gagggtttaa tcccggccat atcaccggcg gaaatatagc
     5341 catttcgacc tctttcaaaa gcaagcccaa agatgaaaag aaggcggcgg aagaaaaaaa
     5401 gggagaagac agccagttgc cgatgaccct cgaagaacaa caggccgaac tgaattatat
     5461 ccggaacaat cccgccgaat ttgttgattt caatattgcc tggtccgtga atatctccta
     5521 ttcgctcaat ttcagccgtg ttctaaggcc cgattacagc ggctacaaga cccagctgac
     5581 ctcttccctg atgctaaacg gcgactttaa cctcacggag aagtggaagg ttagttacag
     5641 tacgtattat gatgtcaaga acctgaaaat acaatccttt accgggtctc tttcccggga
     5701 tatgcattgc tggcaattgt cgatcaatgt tacgccggtt ggtttatacc gttcattcag
     5761 cataaccatt aacccgaaat cagggatcct gcgcgatttg cgcatcaatc ggaaccgcac
     5821 atttaattag gagaatggct taagatgtat tgcttcatca ggtcgtgcac gcggtttttc
     5881 agttgctcca cggtttcccc gggcggggat tttacttcgg gaagaaaatc cagataaagt
     5941 tttacgggaa gcaggaaaaa tcctttcaag ggtggattgg ccctgcgact attgaatatg
     6001 accatgggaa taatggattt cccgctgccg atggccaggc ggaaagcgcc atcgtgaaag
     6061 gattttagcg gttccgtacc ggtgtttctt gttccttccg gataaatgca catgtgcagg
     6121 cccatgttca gtacttcgtg catttttata aaactgtcgc gcctgctttt ttcattt
//
LOCUS       DASYCU010000027         4978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4792401, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000027 DASYCU010000000
VERSION     DASYCU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4792401"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..721)
                     /locus_tag="VGM24_00520"
     CDS             complement(134..721)
                     /locus_tag="VGM24_00520"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEY4153868.1"
                     /translation="MQRDAERYGEKEKFMQALTTRAKTVGRKPMTFWERLYLPAIAKG
                     MSITFGHIFKRKPTISYPEQQREFSPVFRGVQILNRDEEGRERCTACGLCAVACPAEA
                     ITMEAAERLPGEEDLYREEKYAARYEINMLRCIFCGLCEEACPKDAVYLSETLVPSNF
                     NRQSFVYGKEELLIPHPLKEPEAFAKARGQKAARK"
     gene            complement(813..1886)
                     /gene="nuoH"
                     /locus_tag="VGM24_00525"
     CDS             complement(813..1886)
                     /gene="nuoH"
                     /locus_tag="VGM24_00525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY4153869.1"
                     /translation="MHALLLAVDWPLIIEKAVLISIILGISLFVAMYETYAERKVAAF
                     IQDRHGPNRAGPFGIFQPLADGLKLFSKEEIVPNHSNKFLFILGPGLAMLTAMMTSAV
                     VPWGGHIHAFGRDISLQIADVNIGILYIFGVVSMGVYGLMIGGWASNNKFSLLAAIRG
                     ASQIISFELAMGLSLIALLMMTGSLSLHTIVEQQQQGRYYVLYQPVGFVIFLVCAFAE
                     CNRTPFDLSEAESELNFGYHQEYSSMKLGFYLFAEYVNMFISSAVMATVFWGGYDIPF
                     VNDAAFGLDHGANLLALLQVLSLFVKIGLFIFFFMWVRWTVPRFRYDQLMRLGWKTLI
                     PLALLNMLLTGFVILLKQNHWHF"
     gene            complement(1923..3020)
                     /locus_tag="VGM24_00530"
     CDS             complement(1923..3020)
                     /locus_tag="VGM24_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY4153870.1"
                     /translation="MAEEKKLFKVTIDNIAIEVEPGTSILNAARKIGGEVAPPAMCYY
                     STLKGSGGKCRTCLVEVAAGSTADPRPMPKLVASCRTNVMDGMIVKNVTSEKVLDARK
                     GVVEFLLINHPLDCPICDQAGECSLQDLSYEHGNEGTRYEFKRRTFEKHDIGPYIQLH
                     MTRCILCFRCVYVADQLTSEREHGILDRGDHSEIATYIHKALENDFIGNVIDVCPVGA
                     LTDKTFRFKNRVWFLKPQDAHRDCPKCSGKVTLWNRGDEVYRVTARKDQWGEVKEFIC
                     NDCRFEKKKTADWIIEGPTRISRHSVISQGHYVDTVEPKETLPEVMGGRKPKLLMDIN
                     TVSEVNRPEIDLSEIQGPAHSMDFDLENGGA"
     gene            complement(3039..4400)
                     /gene="nuoF"
                     /locus_tag="VGM24_00535"
     CDS             complement(3039..4400)
                     /gene="nuoF"
                     /locus_tag="VGM24_00535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY4153871.1"
                     /translation="MGRKLLLEKAQVEGIRGYDVYRREGGYRSVEKAFKEMNPDQITD
                     EVKKSGLRGRGGAGFPAGMKWSFIAKPEGVPRHLVCNADESEPGTFKDRYLMEFLPHL
                     LIEGLIVSSYALGSHVTYIYIRGEYSWIIDILETAIAEAKANGWLGKNILGSGFDLEI
                     YVHRGAGAYICGEETALLESLEGKRGNPRIKPPFPAVRGVWDRPTVVNNVETLAAVVP
                     IILMGGEEYAKIGVGKSTGTKLISACGNINKPGVYEIDMTIPVEEFIYSDEYCGGIAN
                     GKKLKACIPGGSSVPILPANLLLKTAKGEKRIMNYESLSDGGFAKGSMMGSGGFIVLD
                     EDQCVVRHTYTLARFYRHESCGQCSPCREGTGWMEKILKNIDTGKGKMSDIDLLWDIQ
                     RRIEGNTICPLGDAAAWPVAAAIRHFRDEFEWHIKNPEESQKRNYGLAHYADPIEPVG
                     AAV"
     gene            complement(4412..4615)
                     /locus_tag="VGM24_00540"
     CDS             complement(4412..4615)
                     /locus_tag="VGM24_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153872.1"
                     /translation="MKKNFRTVILLFAVFSVVLLMGGIWTKDGQLRKMLFWMSAVVAV
                     SLVFQLLLFAIKPGLFRDRSNQS"
     gene            complement(4618..>4978)
                     /locus_tag="VGM24_00545"
     CDS             complement(4618..>4978)
                     /locus_tag="VGM24_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY4153873.1"
                     /translation="MDYVAELLNILPVEVYEVATFYSMYNLKPVGKYIFEVCQTGPCM
                     INGSDEIIQYIRDTLHINVGETTEDGLFTLKTVECLGACGYAPMMQLGKFYRENLTRE
                     KVDGIIRECREKAAANN"
BASE COUNT         1160 a         1425 c         1130 g         1263 t
ORIGIN      
        1 caatgatcag ccacaacaca ctgtgaacgg gattcttact aatgaccacc ataatggcac
       61 tgatcagaga cagtgcgctt aaaaaccaaa acaggaattg ggtcaggctc atgaattaat
      121 tgatattttt aaactatttc ctggcggctt tttgtcccct tgcctttgca aatgcctccg
      181 gttctttcag cgggtgggga atcaacagtt cttctttacc gtaaacaaag gattgccggt
      241 tgaaattgga cggtaccagg gtctcggata aataaaccgc atccttggga caggcttctt
      301 cacacaatcc gcaaaatatg cagcgcagca tattgatctc gtaacgggct gcgtattttt
      361 cttcccggta cagatcttcc tccccgggta aacgctcggc cgcttccatc gtgatggctt
      421 cggcggggca tgctacggca cacaagccgc aggccgtgca ccgctccctt ccttcctcat
      481 cccggttcag gatctgaacg ccgcggaaca ccgggctgaa ttcccgttgc tgttcgggat
      541 aactgatggt cggcttcctt ttgaaaatat gcccgaatgt aatggacatt cctttggcaa
      601 tagccgggag atacaatctt tcccagaatg tcatcggctt ccggccgacg gtctttgccc
      661 ttgtcgttaa tgcctgcata aatttttctt tttctccata ccgttccgca tcgcgctgca
      721 aaaatatttt tatcgactca accttccaat taaattctcc cggcaattca tatccgggct
      781 cattcaccct tcttccggaa acccggcaga atttaaaaat gccagtgatt ctgtttaagc
      841 aggatcacaa aaccggtcag cagcatattc aggagggcca gcgggatcag cgttttccag
      901 cccagcctca tcagctgatc ataccggaac cggggaaccg tccagcgcac ccacataaaa
      961 aagaatatga agaggccgat cttgacaaac aaactcagca cctgcaataa agccagcagg
     1021 ttggccccgt gatccaggcc gaaagcggcg tcattgacaa aggggatatc atagcctccc
     1081 cagaaaacag tagccatcac ggcgctgctg ataaacatat tcacgtattc ggcaaacaga
     1141 taaaacccga gcttcatcga agaatattcc tggtggtatc cgaagttcaa ttcgctctcc
     1201 gcttcagaca ggtcgaaggg tgtccggtta cactctgcaa aggcgcaaac caggaatatt
     1261 acaaatccca ccggctggta caatacataa tatcttccct gctgctgttg ttccacgatg
     1321 gtatgcaggc tgagcgaacc cgtcatcatc aacagggcga tcaggctcag gcccatcgcc
     1381 aattcaaagg aaatgatctg cgatgccccg cggatcgcgg ccagcaggga gaatttattg
     1441 ttggaagccc atccgccgat catcagcccg taaacgccca tgctcaccac gccgaaaata
     1501 tagaggatcc cgatattgac gtcggcgatc tggagggaga tatcgcggcc aaaagcatga
     1561 atatggcctc cccagggcac caccgcactg gtcatcatgg ccgtgagcat ggccagcccc
     1621 ggacccagga taaaaagaaa tttgttggag tggttgggta cgatctcttc cttgctgaac
     1681 agcttcaatc cgtctgccag gggttgaaaa attccaaagg ggcccgcccg gttcggaccg
     1741 tgcctgtcct gaataaaggc cgctactttt ctttccgcat acgtctcgta catggccacg
     1801 aataacgata tgccgaggat gatcgagatc agtaccgctt tctctatgat caaaggccag
     1861 tcgacagcta ataataatgc gtgcatttta tcttaataaa gtttcttcgt ttgtttgcca
     1921 gctcatgctc ccccgttttc caaatcaaaa tccatggaat gcgccggtcc ctggatctcc
     1981 gacagatcga tctccggccg gttcacttca ctcacggtat tgatatccat caggagcttt
     2041 ggctttcttc cgcccatgac ctccggaagc gtttccttcg gttccaccgt atccacatag
     2101 tgtccctgcg agataaccga gtggcggctg atccgggtag gcccttcaat gatccagtcg
     2161 gcggttttct ttttttcaaa ccggcaatcg ttacagataa attctttcac ttctccccac
     2221 tggtctttgc gcgcggtaac ccggtacact tcgtctcccc ggttccagag ggttactttt
     2281 ccggaacatt tcgggcaatc ccggtgggca tcctggggtt tcaaaaacca gacgcggttt
     2341 ttgaaccgga aggttttatc cgtgagcgcg ccaacgggac acacatcgat cacattcccg
     2401 ataaaatcat tttccagcgc tttgtgaata taggtggcta tttcggaatg atccccccgg
     2461 tcgagtatcc cgtgttcccg ttcgctggtg agctggtcgg ccacatacac acaccggaag
     2521 caaaggatgc aacgggtcat gtgcaactgg atataggggc cgatatcatg tttttcgaaa
     2581 gtacggcgtt taaattcata acgcgttcct tcattcccat gctcataact caggtcctgc
     2641 aaagaacatt ctcctgcctg atcgcaaatc ggacaatcca gcggatgatt gatgagcagg
     2701 aattccacca ctcctttgcg cgcgtccagc actttttcac tcgtcacatt ttttacgatc
     2761 atgccatcca tcacgttggt gcggcaactg gccaccagct tgggcatggg tcgcggatcc
     2821 gcggtggacc cggcggccac ttccaccagg caggtgcggc acttgccgcc gcttcctttc
     2881 agggtgctgt aataacacat tgccggaggc gccacctcac cgccgatctt ccgcgccgca
     2941 ttgagaatgc tggtgcccgg ctccacttcg atcgcaatat tatcgatcgt aaccttgaat
     3001 aatttttttt cttcggccat aattcctttt ttcaggttct aaacggctgc ccccaccggt
     3061 tctatcggat ccgcataatg ggcgagcccg taatttcttt tctggctttc ttccggattc
     3121 tttatatgcc attcaaactc gtcccggaaa tgccggatcg ctgcagccac gggccaggca
     3181 gcggcatcgc ccagcggaca aatggtattg ccttctatcc ggcgctgaat atcccacaac
     3241 agatcgatat cactcatttt tccctttccg gtatcgatgt tcttcaatat tttttccatc
     3301 cagcccgtgc cttcccggca gggagagcac tgaccgcagc tttcatggcg ataaaaacgc
     3361 gcgagggtat aggtgtgccg gacgacgcac tggtcttcat ccagcacgat gaacccaccc
     3421 gaacccatca tggagccttt ggcaaaaccg ccgtcggaaa gactctcata attcattatc
     3481 ctcttttctc ctttcgccgt ttttaacagg agattcgccg ggagaatggg cacggaagat
     3541 ccgccgggaa tacaggcctt cagttttttc ccgttcgcga taccgccgca gtattcatcg
     3601 ctgtaaatga attcttccac cggtatggtc atgtctattt catagacccc gggtttatta
     3661 atatttccgc aggcggaaat aagctttgtt ccggtcgatt ttccaactcc gatctttgca
     3721 tattcttctc cgcccatcag gatgatcggc accacggccg ccagcgtttc cacattgttt
     3781 accaccgttg gccggtccca gacaccgcgc acggccggga agggcggttt gatgcgggga
     3841 ttcccgcgtt tgccttccag gctttccagc agggccgttt cttcaccaca gatataagct
     3901 cctgcaccgc ggtgcacata aatttccagg tcgaaccccg aacctaaaat gtttttcccg
     3961 agccagccgt tggcctttgc ttcggcgata gccgtttcca ggatatcaat gatccaggaa
     4021 tattccccgc ggatataaat ataggtgaca tgactgccca gtgcatagga ggaaacgatc
     4081 aggccttcga tcagcaggtg cggcaggaac tccatgagat agcggtcctt gaatgttccg
     4141 ggttcgcttt catctgcatt gcacaccagg tgacggggca cgccttccgg cttggcaata
     4201 aaactccatt tcatgcccgc cgggaaaccg gcgccccctc tgccccgcaa tccgcttttc
     4261 ttcacttcat cggtgatctg gtcggggttc atctccttaa acgctttttc cacactccgg
     4321 tatccgccct cgcggcggta cacgtcgtag ccccggattc cctccacctg cgctttttcc
     4381 aataataatt ttctacccat gctgtataag attaggattg attggatcta tccctgaaca
     4441 atccgggttt tatagcaaac aacaacaatt gaaataccag cgatacggcc acaacggccg
     4501 acatccagaa aagcattttc cgcaactggc cgtctttggt ccagattcca cccatcagca
     4561 ataccacgga aaaaaccgca aacaggagga taaccgttct gaaatttttt ttcatgatca
     4621 attatttgca gccgcttttt ccctgcattc ccggataatg ccatccactt tttcccgggt
     4681 caggttttcc cggtaaaact tgcccagctg catcatcggt gcataaccac aggcgcccag
     4741 gcattccacc gtcttgagcg tgaacaatcc atcttccgtg gtttctccca catttatatg
     4801 cagggtatcc cggatatact ggatgatctc atcgctccca ttgatcatgc aggggccggt
     4861 ctggcagacc tcaaaaatat atttgcccac cggcttcagg ttatacatgg aataaaaggt
     4921 ggccacttca tatacttcca ccggaagaat attcagcagc tcagccacat aatccatg
//
LOCUS       DASYCU010000028         5855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4502400, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000028 DASYCU010000000
VERSION     DASYCU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4502400"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..914
                     /locus_tag="VGM24_00550"
     CDS             246..914
                     /locus_tag="VGM24_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY4153874.1"
                     /translation="MPLFYQHNINESTRLAIWHIEEDEAFFLKRVPLKRDVSHPHKRL
                     QHLAGRYLLTELFSDFPLAEILVADTRKPYLPGEKYHFSISHCGSFAAAIVSLRFRVG
                     IDVEQAGPAIGRIREKFLSADELAMACREPEHSGQWLRQLTLLWSAKESIFKWYSLGG
                     VNFREHIQWLEPCFTRAEEAPGKLGFAFLKGGNQDLTVHYKLFNDLVLSFVYSRDRLA
                     PDNA"
     gene            1038..3464
                     /locus_tag="VGM24_00555"
     CDS             1038..3464
                     /locus_tag="VGM24_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4153875.1"
                     /translation="MAYRLTNYRYPDSFVPLPMIKNIFKVALRNLVRNKIYSFINIAG
                     LATGMAVALLIGLWIHDELSFNKGFDHYNRVGQLWQFVKFDAEKSSYNVMPIPLAKEL
                     RSKYPEFKYVSLSSNKKFVLAAGDKQLTETGNYVEPDFAEMMSLKMLSGSRSGLNGMN
                     NILLSESLAKAFFGSANPINRLIKIDNKSIVKVAGIYRDFPDNSSFKEVHLLATWELY
                     AATDEGVKNSTPAWDDNSYEIYAQLQEGTDFKKVSAKIRDIRMKMDNPPGYKPEFFVF
                     PMSRWHLYSDFKDGVNTGGLISFVRLFAVIGIFVLLLACINFMNLSTARSEKRAKEVG
                     IRKTIGSARWQLITQFLGESLLMVASALVLSLILAQLALPFFNEVSGKQITIPWSDPF
                     FCLAGLGFSLLTGLIAGSYPAFYLSSFRPVKVLKSVFRAGRLATIPRKVLVVLQFTVS
                     VILIIGTIVVFRQIEFARDRPVGYNRNRLIEVSMNTPELSQHFDALRTDLLNTGAVSA
                     FSESSCPISRQWGGTTDTWWKGKNPQAQPLMMANSVTHDFGKTVGWQLEDGRDFSHQF
                     STDTSAIILNESAVKTIGFKHPLEGSVTVHGKPYQVIGVIKDMIRESPFEPVKPTFFL
                     LDYRNLSVIDIKLAPQSGTAEALGKIEKVFRKHNPGSPFTYSFVDADYNSKFTDEERI
                     GRLARFFAVLAIFISCLGLFGLASFVAEQRTREIGVRKVLGATVLNVWVLLSGEFLLL
                     VGIAFLIAVPVAYHFMYKWLQNYGYRTSLSTWIFLAAGAGTILITLITVSFQAIRAAL
                     ANPVKSLRTE"
     gene            complement(3498..4748)
                     /locus_tag="VGM24_00560"
     CDS             complement(3498..4748)
                     /locus_tag="VGM24_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEY4153876.1"
                     /translation="MSTIKCPNCHHEFAMEEAVSEQYKKDLREQMISFTRKKEEEFQQ
                     KSADFSKQLQLQQDLFNKKLNEEKNQLRLSLEENLRRTISTDFENRLALLQQSNNEQE
                     EKLKQARQKELELLQHEQELKNKAAEMELSVQRILREEREKMAGEIRGIEEQKTAAKE
                     TEHQLRLKEKEKQLDDQKRLIEELQRKAEQGSMQLQGEVQELALETLLRASFPFDLVA
                     PVGKGVRGADCSLTVRNPLGQECGKIIYESKRTKNFEADWVDKLKHDMRQQGAHLAVL
                     VTRVMPKDMECFGEKNGVWICNFSEVKALSQVLRDGIIRIFQAARTQQNKGDKMQLLY
                     DYLTGSEFSEQWKAVREGFSSMKMSIQRERDMMEKLWKAREKQLEKVLLNAAHIKGSV
                     EGIAGQDTFSMDLIDDTDEILGIE"
     gene            4819..>5855
                     /locus_tag="VGM24_00565"
     CDS             4819..>5855
                     /locus_tag="VGM24_00565"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY4153877.1"
                     /translation="MKYLFPVAFLFFFTTGFAQQTEKPPLHAKHWMAITGKPLGAEAG
                     SQIFQKGGNAVDAACAMLASVCTMWDVLSWGGETQAIIYNPKTKKVISLDALGFAPGA
                     ATAAFYKSKGYYFPPEYGPLAAVTPGTPGGLCYLLAEYGTMSLKEVLAPAMQLAAGYP
                     MEAQTANSIENNLKRIKEWPYSKKIFITHPGEKREAPEAGEIFVQQDLLNTLTKMVEA
                     EQDALKKKKSRKEAIFAASDRFYKGDIAREFVRGCREQGGIISMEDLARWKPVEEEPL
                     HVNYKGIDVYKLQQWSQGPALLQSLNILENFNLKEMGYNSTRYIHTLYQAMNLAFADR
                     DFYYGDPYLPP"
BASE COUNT         1332 a         1501 c         1475 g         1547 t
ORIGIN      
        1 tatgtttttt cgcgtccagg atatgctccc ggtaagtagc cagccattta cccagatgaa
       61 catcatagga gttgctgccc aggcaactcc ggttgtacgg ttccactttg atggttccct
      121 ttcccatttc ctccaggata cgcttgtcag ataaaatcat tgagtgtgac ggtttatttg
      181 tttatttgtt ccggtcttta ttccgcaccg gaatgcaggc gaatttcgcg ataagctttt
      241 tttggatgcc cttattttat caacataata ttaacgaatc tacgagactg gctatctggc
      301 atatcgaaga ggacgaggct ttttttctta aaagggtgcc gttgaaaaga gacgtgtccc
      361 atccgcacaa gagacttcag cacctggccg gccggtacct gctgaccgaa ttgttcagcg
      421 attttcccct tgccgaaatc ctggtggccg atacgcgtaa gccgtatctg cccggggaaa
      481 aatatcattt ttccatttcg cattgcgggt catttgctgc cgccatagtc agccttcgtt
      541 tccgggttgg gattgatgtg gaacaggccg gtccggcgat cgggcggatc cgggagaaat
      601 tcttatcggc agatgaactg gcgatggctt gccgggagcc ggaacattcc ggtcaatggc
      661 tgcgccagct gaccctgctc tggtctgcca aggaatccat attcaaatgg tacagccttg
      721 gcggcgtgaa tttccgcgaa catatccaat ggctcgaacc ctgttttacc cgggcagagg
      781 aagcgcccgg gaagctgggc tttgcttttc tcaagggagg aaaccaggac ctcacggtcc
      841 attacaagct gttcaatgat ctggtgctaa gctttgttta ttcccgggac cgcctggctc
      901 cggataatgc ataataaaag cagcatcgtt ttgccaggtg ggtgagccgg atttaaaacc
      961 ggtcggttgt gtatcaaaaa tgaacaatct ccgggttgtc cgggtcctct gctccctaag
     1021 tatcaattgg ataggatttg gcatatcgct tgacaaatta ccggtacccg gacagctttg
     1081 tcccgcttcc catgatcaaa aatattttca aggtcgctct gcgaaacctg gtcagaaaca
     1141 agatttattc tttcatcaat attgccggcc tggccaccgg tatggcggta gccctactca
     1201 tcggactttg gattcatgac gagctcagtt tcaacaaagg ctttgaccac tacaaccgcg
     1261 tcggacagct ctggcaattt gtaaaattcg acgcggagaa atcttcctac aacgtcatgc
     1321 ccattccgct ggcaaaagag ctgcgcagca aatatcccga gttcaaatat gtaagtcttt
     1381 cttcgaacaa aaaatttgta ctggctgccg gggataagca attgacggaa accggcaatt
     1441 atgtggaacc ggattttgct gaaatgatgt cgctgaagat gctgtcggga agccgcagcg
     1501 gcttaaacgg aatgaataat atacttcttt ccgaatccct ggcaaaggct ttttttggtt
     1561 cggcgaatcc catcaaccgg ctcattaaga tcgataataa atccattgta aaagtcgccg
     1621 ggatttaccg ggactttccc gataacagca gtttcaaaga ggtgcatttg ctggccacct
     1681 gggaattgta cgccgcgacg gatgagggcg tgaagaattc cacaccggcc tgggatgaca
     1741 attcttacga gatctatgca cagcttcagg aagggactga ttttaaaaaa gtatccgcca
     1801 agatccggga catccggatg aaaatggata acccgcctgg ctacaaaccg gaatttttcg
     1861 ttttccccat gagccgctgg catttatatt ccgattttaa ggacggggtg aataccggcg
     1921 gactcatcag ctttgtccgg ctctttgccg tcatcggtat ttttgtgctg ttgctggcct
     1981 gcatcaattt catgaacctg agtacggcgc gctctgaaaa gcgcgcaaaa gaagtcggca
     2041 tccggaaaac aataggttcc gcgcgctggc agctgatcac ccagttcctg ggcgaatcgc
     2101 tcttaatggt agcttctgca ttggtattgt cgctgatcct cgcgcaactg gcccttcctt
     2161 ttttcaatga agtatccggc aaacagatta ccatcccgtg gtctgatcca tttttctgtc
     2221 ttgccggcct ggggttcagt ttgctgaccg gactgattgc cggcagttat ccggcatttt
     2281 atttatcgtc tttccggccg gtaaaagtat tgaagtcggt attccgcgcc gggcgcctgg
     2341 ccacgattcc ccggaaagtg ctggtcgtgc tgcagttcac cgtctcggtg atcctgatca
     2401 ttggcaccat cgtcgttttc cgccagattg aatttgcgag ggaccggccc gtaggctaca
     2461 accgtaaccg gttgattgag gtaagcatga atacaccgga actttcccag cattttgatg
     2521 cccttcgcac ggatctcctg aataccggag ctgtttccgc attctccgaa tcctcctgcc
     2581 ccatttcaag gcaatgggga ggcacaacag atacctggtg gaagggaaaa aacccacagg
     2641 cgcaaccgct gatgatggcc aattcggtga cgcatgattt cggtaaaacg gtgggctggc
     2701 agttggagga tggaagggat ttttctcatc agttttcaac cgatacgtca gcgatcatcc
     2761 tgaatgaatc cgccgtaaag accatcggat tcaagcatcc gctggaggga tcggtgaccg
     2821 tgcatggcaa accataccag gtgatcggcg tgataaagga tatgatccgg gaatctccct
     2881 tcgagccggt gaagccgact ttttttcttc tggattaccg gaacctcagt gtgatcgata
     2941 ttaaactggc cccgcagtcc ggtactgccg aagcattggg gaaaatagaa aaggtattcc
     3001 ggaaacacaa tccgggcagc ccctttacct attcctttgt ggatgcggat tacaactcca
     3061 aattcacgga tgaggaacgg atcggcaggc tcgcccgctt ttttgccgtc ctcgccattt
     3121 tcatttcctg cctgggattg tttggactgg catccttcgt tgccgagcag cgcacccgcg
     3181 agatcggcgt aaggaaagta ctcggggcaa cggtattgaa tgtctgggtt ttgttgtcgg
     3241 gcgaattcct cctgctggtg ggcattgctt ttctcattgc cgtaccggtc gcctatcatt
     3301 tcatgtataa atggctgcag aactacggtt accgtaccag cctgagcacc tggatttttc
     3361 tggcagccgg cgcggggaca atactgatca cgctgatcac cgtcagcttc caggcgatcc
     3421 gggcggcgct tgcgaatccg gtaaagagtc ttcgtacgga ataaaaaggg gactcccttt
     3481 ttggaccggc catctggcta ttcaatcccc aaaatctcgt ccgtatcatc gatcaggtcc
     3541 atgctgaacg tatcctgtcc tgcaattcct tccacggaac ctttaatatg cgccgcattc
     3601 agcagcactt tctccagctg cttttcccgg gccttccata gtttctccat catgtcgcgt
     3661 tcgcgctgga tggacatttt catggatgaa aatccttcgc gcaccgcctt ccactgttcg
     3721 ctgaattccg aaccggtaag atagtcgtat agcaactgca tcttgtcgcc tttgttctgc
     3781 tgggtccggg cagcctgaaa aatccggatg attccgtcgc gcagaacctg cgaaagggct
     3841 ttgacctcgg aaaaattaca gatccatacg ccgtttttct ccccaaaaca ttccatgtcc
     3901 tttggcatga cccgggtgac cagcaccgcg aggtgggcgc cctgctgacg catatcgtgt
     3961 ttcagtttat ccacccagtc cgcctcaaag tttttcgttc ttttactttc ataaataatt
     4021 tttccgcatt cctggccaag cggattccgg acggtaagtg aacaatccgc cccgcgcacg
     4081 cctttcccga ccggcgctac gaggtcgaat ggaaaagaag cccgtaataa ggtttcgagg
     4141 gccagttcct gcacttcccc ctgcaattgc atggagccct gttctgcttt gcgctgcaac
     4201 tcttcaatga gtcttttctg atcgtcgagc tgcttttctt tttctttcag ccggagctgg
     4261 tgctcggttt ctttggccgc ggtcttctgc tcttcgatgc cgcggatttc acctgccatt
     4321 ttttcccgtt cttcccgcag gatccgctga accgacaatt ccatttcggc tgctttattt
     4381 ttcagttcct gctcgtgctg caggagttcc agttctttct gccgggcctg tttcagtttc
     4441 tcttcctgct cgttattgga ttgctgaagc agggcaagcc ggttctcaaa atcggtgctg
     4501 atggttctgc gaagattttc ttccagggac agccggagct ggtttttttc ttcattcagc
     4561 ttcttattga aaagatcctg ctgcagctga agctgtttcg aaaaatcagc cgacttctgc
     4621 tggaattcct cttctttttt ccgggtgaat gagatcatct gttcacgcag gtcctttttg
     4681 tattgttcag acacggcttc ctccatcgca aactcatggt gacaattcgg gcacttgatg
     4741 gttgacatga atgtttttgg ggtaaagtta aggaagggat gacggatggt ttcttacctt
     4801 taagaacaaa taccgcttat gaaatatctc ttcccggttg ccttcctgtt ttttttcaca
     4861 accggttttg cccagcaaac ggaaaaacct ccgctgcatg caaaacactg gatggcaata
     4921 accggaaagc cgctgggagc cgaagccggg tcccagatct ttcagaaagg cggcaacgcc
     4981 gtggacgccg cctgtgcaat gctggcgtcc gtttgcacga tgtgggatgt gctgagctgg
     5041 ggaggggaaa cgcaggccat catctataat cccaaaacga agaaagtgat ttctctggat
     5101 gccctgggtt ttgctcccgg agcggcaaca gctgcttttt ataaaagcaa aggatattat
     5161 tttccgccgg aatacgggcc tctcgcagcg gtgacgccgg gcacgcccgg agggctttgt
     5221 tacctgctgg ctgaatatgg aacaatgagc ctcaaagaag tgctggcacc cgccatgcaa
     5281 ctcgcagccg gctacccgat ggaagcgcaa accgccaaca gcatcgaaaa caatctgaag
     5341 agaatcaagg aatggcctta cagcaaaaag atcttcatca cccatcccgg tgaaaaaaga
     5401 gaagcgccgg aggcaggcga aatttttgtt cagcaggatc ttctgaacac gcttaccaaa
     5461 atggttgaag cggaacagga tgcgctgaaa aaaaagaagt cgcggaagga ggctattttt
     5521 gcggcttccg accggttcta taaaggagat atcgccagag aatttgtgcg cggttgccgg
     5581 gaacagggcg ggatcatctc gatggaagac ctggcccgct ggaagcccgt cgaagaagaa
     5641 ccgttgcatg tgaattataa aggcattgac gtatataaac tccagcagtg gagccagggg
     5701 ccggccctgt tgcaaagcct gaacatcctg gaaaatttta atctgaaaga gatgggctat
     5761 aacagtaccc gttatatcca taccctttac caggccatga acctggcttt tgccgaccgg
     5821 gatttttatt atggcgatcc ttatctgcct cccga
//
LOCUS       DASYCU010000029         4552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_589343, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000029 DASYCU010000000
VERSION     DASYCU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589343"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VGM24_00570"
     CDS             <1..1432
                     /locus_tag="VGM24_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY4153878.1"
                     /translation="ANCGAHSLTPFPTTPGVWSPRNGTGGEGCNMAAIKIAFNFAGVG
                     ADPRSLINGHYDSSGCVPLDVMFVDTLRNAKQYDWNFGDGSPDTITAAYQVNHTYTST
                     GTFHVRLIAIDSNSCNVRDTAYLNIRVRTDKAVLDFDITKLPPCESLNFQFENKSTAP
                     PGKPFTPDDFTWDFGDGTRVAGTGVITHAYAASGTYPVRLVLLDTSYCNYPDSLVKEL
                     RVSPLVKAQFELPDGCAPYEAAFNNTSLAGQQFFWDFGDGTTSTDVNPVHIYANPGSY
                     TVSLLAIDNGTCNIRDSTTRTIQVHEKPAAAFTTRPIPPEYNVPTVFENSSTGATRYT
                     YFFGDGDSTDKDNTDTVVHQYTETGSYEACLVAYNQYGCPDSACHNVDVLINPLLDVP
                     NAFTPGRFGQNSTIMVKGFGIESMDWKIYNRWGQVVFHSNSPYYGWDGTWKGVAQPMD
                     VYTYTLEATLSDGTKTTRKGDITLIR"
     gene            1473..2513
                     /locus_tag="VGM24_00575"
     CDS             1473..2513
                     /locus_tag="VGM24_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEY4153879.1"
                     /translation="MIRTRKLLLLLTVPLFLGLSLEAQDLHFSQWFNSPLTTNPANTG
                     FIPDADYRLGANYRNQWSSIMAQPYKTMGVWGDAQVFRDKIQSGWLGLGGVILHDVAG
                     TGSLSTTEIYGSVAYHQMLGYSSLLSIGFNTGYVNKSINNSNLKFPDQFDGKFFDAKM
                     PTSVIIDQPNVNYFDMQVGMNYAFFPNKNKYINVGFSVQHINGARETFFNTDVAGYDD
                     RIRPRYIAFINSSFKTSDQVIINPMGYYTTQAGASEAVIGLNVQYNLSGDGDQQLIGG
                     LYYRVSESVVPMIGFVYKNIRLMFTYDVTTSSLKQYNNGNGAWEFAVIRPGDYSEYNG
                     DRHQSLCPTFRN"
     gene            2549..3106
                     /locus_tag="VGM24_00580"
     CDS             2549..3106
                     /locus_tag="VGM24_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153880.1"
                     /translation="MKKLGSLLVVTFALLQFAVAQQSGPPQGFDKSKLFFGGNFGLNF
                     GTYTIINVSPQVGYHFTPQFAAGAGVNYIYYGYNDHYDLLKYTQSYAGLNVFGRFYPI
                     QQFFIQAQPELNYVWGKIRYYGAEQSFTKIPTQFVPSLLLGGGAAIPAGRGAIMISVL
                     YDVLQNTYSPYYHQALFGLGYNIGF"
     gene            complement(3103..3642)
                     /locus_tag="VGM24_00585"
     CDS             complement(3103..3642)
                     /locus_tag="VGM24_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY4153881.1"
                     /translation="MNIESVAVFCGSHLGNNPVYAEHVKELGKLLAMLKVRVVYGGGH
                     KGLMGVLADSVLAQEGHIMGIIPQLLIESEQQHKGLSEMAVVPDMHTRKKMMYERCDA
                     VIVLPGGFGTLDELFEILTWNQLKIHDKKIYILNSDGYYVHLQKHLNQMKKEGFLLGE
                     PAEKIFFCNSPVELFNRIS"
     gene            3726..>4552
                     /locus_tag="VGM24_00590"
     CDS             3726..>4552
                     /locus_tag="VGM24_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY4153882.1"
                     /translation="MSPALLFTFVIAYFALLLFVAWRTSKGSDNESFFIGNRNSNWML
                     VAFGMIGTSLSGVTFVSVPGAVARESFAYFQITLGYFLGYLLIAFVLLPLYYRLRLTS
                     IYNYLNERLGTRAYKTGAGFFILSRVLGATARLYLVVNILQEAILSSFHVPFWLTTLI
                     ILLMILLYTYEGGVKTIVWTDTLQTTCMLGGMVVCVIYILNHLNLNLSGAMEAMQAKG
                     YSKIFFTDPDSKLFFLKQIVAGMFITLTMTGMDQEMMQKSISVRTLKDSQKNMVVLG"
BASE COUNT         1076 a         1190 c         1111 g         1175 t
ORIGIN      
        1 cgccaattgc ggggcacata gtctcacgcc cttccccacc acccccggag tatggtcgcc
       61 gcgaaacgga accggaggag aaggatgcaa tatggccgcc atcaagatcg catttaattt
      121 tgccggagtg ggggccgacc cgcgctccct gatcaatggt cattacgaca gctcgggttg
      181 cgtaccgctc gatgtaatgt ttgtggacac cctgcggaac gccaaacagt acgattggaa
      241 tttcggcgat ggcagcccgg ataccatcac cgcggcgtac caggtgaatc atacctatac
      301 ttccaccggc acttttcacg tgcggctgat cgccattgat tccaattcct gtaatgtgcg
      361 ggatacggcc tacctgaata tccgggtgcg gacggataaa gccgttctgg attttgatat
      421 caccaaactt ccgccctgtg agtccctcaa ctttcaattc gaaaataaat ccaccgcgcc
      481 gccgggtaag ccgtttacac ccgatgattt cacctgggat ttcggagacg gtacccgggt
      541 agccggtacc ggcgtcatta cgcatgctta tgccgcttca ggcacctatc cggtgaggct
      601 ggtattgctg gatacgtctt attgcaacta cccggacagc ctggtaaagg aactgcgggt
      661 ttccccgctg gtcaaggccc agtttgagct gcccgatggc tgcgcccctt atgaagcggc
      721 cttcaacaac accagcctgg ccgggcagca gtttttctgg gattttgggg atgggaccac
      781 gtcgacggac gtaaatcccg tccatattta cgcgaacccg ggttcttata cggtttccct
      841 cctggccatc gacaacggaa cctgcaatat ccgggattcc accacccgga ccatccaggt
      901 gcatgaaaag cctgcggcgg cattcactac ccggcccatt cctccggaat acaatgttcc
      961 gaccgtattt gagaacagct ctaccggggc aacacgctat acctatttct tcggggacgg
     1021 ggattcaacg gataaagaca ataccgatac cgtcgtccac cagtataccg aaacgggttc
     1081 ttatgaggcc tgcctggttg cctataacca gtatggttgc cccgattcgg catgccataa
     1141 tgttgacgtg ctgatcaatc ccctgctgga cgtgccgaac gcctttacgc cgggccgatt
     1201 cgggcagaac agtacgatca tggtgaaggg attcggtatc gaaagcatgg actggaagat
     1261 ctataaccgg tgggggcagg tcgttttcca ttccaacagt ccgtattacg gctgggacgg
     1321 aacctggaaa ggggtggcgc agccgatgga tgtgtacact tataccctcg aagcaacact
     1381 gagtgatggt acaaaaacaa cccgcaaagg agatattacg ttaatcaggt aagatgtatc
     1441 tttatcaagg ccattcgaga aattctgtcc cgatgatacg gaccaggaaa ttattgcttc
     1501 ttttaacagt tccgttgttt ctgggtctat ccctggaggc acaggatctt catttttccc
     1561 aatggtttaa ctcgcccctg accaccaacc cggccaatac gggtttcata ccggacgcgg
     1621 attaccgttt gggtgcgaat taccggaacc agtggtcctc catcatggcc cagccctata
     1681 agaccatggg ggtttgggga gatgcccagg ttttccggga taaaattcag tcgggctggc
     1741 ttggattagg cggagtcatt ttgcacgacg tggccggcac gggcagcctg tccaccaccg
     1801 agatctacgg atccgttgcg tatcatcaga tgctggggta ttccagcctg ctttccattg
     1861 gattcaatac cggctatgta aataagagca tcaacaattc caacctgaaa ttcccggatc
     1921 agttcgatgg aaagttcttc gatgcgaaaa tgcccacctc ggtcattata gatcagccca
     1981 atgtcaatta ttttgacatg caggtcggca tgaactatgc tttttttccg aataaaaaca
     2041 aatatatcaa tgtcggattc tccgttcagc atatcaacgg cgccagggaa acctttttca
     2101 ataccgatgt agccgggtat gatgaccgca tccggccccg gtacatcgcg ttcatcaatt
     2161 ccagtttcaa aaccagcgac caggtgatca tcaaccccat gggatattat actacccagg
     2221 ccggcgcatc ggaagcggtg atcggattaa atgtccagta taatttatcc ggagacggag
     2281 atcagcagct gatcggcgga ttatactacc gggtgagcga atcggtggtt cccatgatcg
     2341 ggtttgttta taaaaatatc cggctgatgt tcacgtatga tgtcactact tcttccctta
     2401 agcaatacaa caatggaaat ggcgcctggg aatttgccgt tatccgtccg ggcgattatt
     2461 cagaatataa cggggaccgg catcagtccc tgtgcccgac gttcagaaac taaccgcaga
     2521 aataattaat tcatctaaaa tccggaaaat gaaaaagctt ggatctctgc tggtcgtcac
     2581 attcgcctta ttacagtttg ccgtggcgca gcaatccggc ccgccgcagg gctttgacaa
     2641 atccaaatta ttcttcgggg gaaatttcgg gctgaacttc ggcacctata ccatcattaa
     2701 tgtttctccc caggttggtt atcatttcac gccgcagttt gccgcggggg ccggcgtaaa
     2761 ctatatctac tacggctata atgatcacta cgacctgctg aaatatacac agagctatgc
     2821 cgggctgaat gtttttggcc ggttctaccc cattcagcag ttttttattc aggcacaacc
     2881 ggaactgaac tatgtctggg gaaaaataag atactatgga gcggaacagt cttttacaaa
     2941 aatacctacg cagttcgttc cttccctttt gcttggcgga ggggcagcca taccggcggg
     3001 ccgcggcgcg atcatgatca gcgtgctgta cgatgttctt caaaacacgt attccccgta
     3061 ttaccaccag gcccttttcg gactgggata taatatcggc ttttagctga tccggttaaa
     3121 aagttcaacc ggcgagttgc agaaaaaaat tttttctgcc ggctccccga ggagaaatcc
     3181 ctcctttttc atctggttga ggtgtttctg gagatgaaca taatatccat ccgagttgag
     3241 gatatagatt tttttgtcgt ggatcttcag ctggttccag gtaaggattt caaataattc
     3301 atcgagcgta ccaaaacccc cgggcaatac gatcacggca tcgcatcgct cgtacatcat
     3361 tttttttcgg gtatgcatat ccggtacaac ggccatttcg gaaagtccct tgtgctgctg
     3421 ttcggattcg atgagcagtt gaggaatgat gcccatgata tggccttcct gagccagcac
     3481 cgaatctgcc aggaccccca tcaggccttt atgcccgccg ccgtatacca ctctcacctt
     3541 gagcatggcc agcagtttgc ccagttcttt tacgtgttct gcataaaccg gattattccc
     3601 caggtgggac ccgcaaaaaa cggctaccga ctcgatattc ataagatgtt gctcctttca
     3661 gaaaactaaa ttgaaattta aatattgcat ttcctaattt tcaatcacta atttagcacc
     3721 cttacatgtc accggcttta ctatttactt tcgtcatagc ctattttgcc ctgctccttt
     3781 ttgttgcctg gcgcacttca aaaggatcgg acaacgaatc cttttttatc ggcaaccgca
     3841 attccaactg gatgctggtg gcattcggga tgatcggcac gtccctgtcg ggcgtaacct
     3901 ttgtaagcgt tccgggcgcg gtggcgcggg agtctttcgc ctattttcag atcacgctgg
     3961 gttatttcct gggctatctc ctgatcgctt ttgtgctgct gccgctgtat taccggctcc
     4021 ggctcacctc catttataat tatctcaacg aaaggctggg cacccgggca tataaaaccg
     4081 gcgccggatt tttcattttg tcccgcgtac tgggggccac ggcgcgttta tacctggtcg
     4141 tgaatattct gcaggaagcc atcctttcca gttttcatgt tccgttctgg ctcaccaccc
     4201 tgatcattct tctgatgatc ctgttgtaca cctatgaggg cggtgtgaaa accattgtat
     4261 ggacggacac gctgcaaacc acctgcatgc tgggcggaat ggtggtttgt gtgatctata
     4321 tattgaatca cctcaacctg aacctgtccg gagccatgga ggcgatgcag gccaaaggat
     4381 attccaaaat ctttttcacg gacccggaca gcaaattgtt ttttctgaaa caaatcgtgg
     4441 ccggcatgtt catcacgctc acgatgaccg ggatggacca ggagatgatg cagaagagca
     4501 tctcggtaag aacactgaag gattcccaga aaaatatggt ggtgctcggg tt
//
LOCUS       DASYCU010000030         2680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_879725, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000030 DASYCU010000000
VERSION     DASYCU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879725"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /locus_tag="VGM24_00595"
     CDS             complement(<1..1690)
                     /locus_tag="VGM24_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153883.1"
                     /translation="MPDKLYFSFFIAGMLLLSKAHSQDLGSIGSQKPFAVNGSAQLRG
                     IFYNASGIADRRTPFSYILSGNLNFNLYGVSLPANFTFSEQDRSFHQPFNQFGLSPHY
                     KWITVHLGYRNINFSPYTLAGYTMLGSGVELNPGKFRFGFMYGRLNRATTLDTSSLSL
                     VPISFTRKAYAIKIGYGTENNFIDLSMLKGKDDPSSVNAGKNMLDSLNVLPAANTVAG
                     ISARLTLYKKIFLEGDAATSIYTRDIHSPVSLDSIGDRFIKNIRSVAIVNGSSELCTA
                     MRAAIGYKDKLYTIKIQYQRIDPDFTSMGAYFFNSDIESYSLAPSFHLFRNKLRFNGS
                     IGFQHDNVQRQKEATSKKIIGTAMLSADFTKQLGIDINYSNFSNNQQPQTIRFADSVK
                     IVQNTQNISIAPRFMMANAISSHTVILSANLMQMNDFNNYFAQNAISRNINYQQFYLN
                     YTYGYIPSNFSISLNLSNTTANAAGTTDQNAGATLGINKSFFKSAFLVSLSGGYFLGK
                     RNGTDENTLNFSSNLQYKFLKRQSLNALFYYTNDRPKNISLLSPAFSEIRAELAY"
     gene            complement(1912..>2680)
                     /locus_tag="VGM24_00600"
     CDS             complement(1912..>2680)
                     /locus_tag="VGM24_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4153884.1"
                     /translation="ALGIIALLLYFIYLNKEKLNKILNRKNDQLGELNQQLATANETK
                     ARLFGIIGHDLRAPVGKIVQLLQLQKENPERLNEESRQKHEEKLKTASENVLDTMEDL
                     LLWSKSQMQHFTPEFFPVKIADTVNKEISFLQQPAEDKSLQISNRIPAGFIQTTDENF
                     LSVIVRNLLQNSVKYGVPGSTIVIEASGQRILITNETKQAMSEELNQRLLNNQVNSKR
                     SGMGLQISKDLASAIQAKISFHQPDALHLTAVISWEK"
BASE COUNT          681 a          570 c          675 g          754 t
ORIGIN      
        1 tgtatgcgag ctccgcccgg atttcagaga aggccggcga cagcagggat atatttttgg
       61 gccggtcgtt ggtatagtag aacaacgcat tcaaactctg cctcttcagg aatttatact
      121 gcaggttaga agaaaagttg agggtatttt catcggtgcc atttcgtttg ccaaggaaat
      181 agccgccgct cagggatacc aggaatgcgg atttaaaaaa agatttattg atgcctaaag
      241 tagctcccgc attctgatcg gtagtacccg ccgcatttgc cgttgtattg ctcaggttta
      301 aagaaatgga gaaattcgaa ggaatatatc catacgtgta gttgagatag aattgttggt
      361 agttgatatt cctgctgatg gcgttctgtg caaaataatt attgaaatca ttcatctgca
      421 tcaggttggc agacaggatc acggtgtgtg aagaaatggc attggccatc atgaatctcg
      481 gagcaatgga aatattctgg gtgttctgga caatcttgac ggaatcagca aaacggatgg
      541 tctgcggctg ctggttattg gagaaattac tgtagttaat atctatcccc agttgtttgg
      601 tgaaatcagc gctaagcatg gctgttccga tgatcttttt tgaagtcgct tctttctgcc
      661 gctgcacatt gtcgtgctga aacccgatac ttccgttgaa ccgcagttta ttcctgaaca
      721 ggtggaagct gggcgccaga ctgtaacttt ctatatcgct gttaaaaaag taggcgccca
      781 tggaggtaaa atccggatca atccgctggt attgaatttt gatggtatat aatttgtcct
      841 tatacccgat ggcggcccgc atagccgtgc ataattcgct gcttccgttc acgattgcca
      901 ccgatcttat atttttgata aagcgatccc cgatggaatc cagggacaca ggggaatgga
      961 tatcccgggt gtaaatgctg gtggccgcat cgccttccag gaatattttt ttataaaggg
     1021 tcagccgggc gcttattccc gctacggtat tggccgccgg taatacattt aaagaatcca
     1081 gcatgttctt tccggcattt acgctggacg gatcgtcctt tcccttcagc atactgaggt
     1141 caataaagtt attctctgtg ccataaccga tcttgatcgc ataggctttt ctggtaaagg
     1201 aaataggcac caggctcagg ctgctggtat ccagcgtggt ggcccggttc aggcggccat
     1261 acataaagcc gaaccggaat ttgccggggt tcaattcgac cccggaaccc agcatggtat
     1321 atccggccag cgtatagggg gagaaattta tattccggta acccaggtga acggtgatcc
     1381 atttgtagtg cggactcaga ccaaactggt taaagggctg gtggaaggac ctgtcctgtt
     1441 cgctgaaagt gaaattggca ggaagggaaa ccccgtaaag attgaaattg aggtttccgg
     1501 agagaatata ggaaaatggc gttcgcctgt ccgctatccc ggaagcatta taaaaaattc
     1561 ctcgcaattg cgccgagcca tttacggcaa atggtttttg agatcctatg ctccccaggt
     1621 cctgtgaatg cgctttggaa agcagcaaca tgccggcaat gaaaaaagaa aaatataatt
     1681 tgtctggcat agattatcgg ctagggttcc ttaattcgat atcaggtaga ggataatggc
     1741 caatagaaaa cagatgattg acagaatcag gaaaagcgat tttatccatt ttgtgcttcg
     1801 cattccggcc ataaccgtca actttaatga tttaaagatg gggcctaaaa aaagacgcgc
     1861 gcggaaaagc cgacaaacgt cccttttaaa tcgtcaaaag aaaatgtagg attatttttc
     1921 ccagctgata acagcggtga ggtgaagggc atccggctga tggaaggaga tctttgcctg
     1981 aatggcggat gccaggtctt tagagatctg caatcccata ccggaacgtt tgctgtttac
     2041 ctggttattt agcagacgct ggtttaattc ttccgacatg gcttgctttg tctcattggt
     2101 gatcagtata cgctgaccgc tggcctcaat gacgatcgtg cttcccggca cgccgtattt
     2161 aacggaattc tgaagcaggt tccggacgat caccgataga aaattctcat cggtggtttg
     2221 aataaaaccg gccggtatgc ggttgctgat ctggagactt ttatcttcag ccggttgttg
     2281 caggaagctt atctccttgt tcaccgtatc tgcaattttc acgggaaaga attccggagt
     2341 gaaatgttgc atctggctct tgctccagag cagcaggtct tccatggtat cgagtacgtt
     2401 ttcggaagcg gtttttaatt tttcctcgtg cttttgcctg gattcttcat tcagccgttc
     2461 cggattctcc ttttgcagtt gaagcaattg tacaatttta ccgacgggag cgcgcaggtc
     2521 atgcccgatg atgccgaata acctggcctt ggtttcattg gcagtggcca gctgctgatt
     2581 caattcgccc aattgatcgt tcttccggtt taaaattttg ttgagctttt ctttattcag
     2641 gtagataaaa tacaaaagca gggcgataat gcccaacgcg
//
LOCUS       DASYCU010000031         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1459426, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000031 DASYCU010000000
VERSION     DASYCU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459426"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..823)
                     /gene="rlmB"
                     /locus_tag="VGM24_00605"
     CDS             complement(74..823)
                     /gene="rlmB"
                     /locus_tag="VGM24_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY4153885.1"
                     /translation="MKPSSLIIGRQPLVEAMQSGRGIDKILFQRNVSGESIGEIRKLA
                     EKRGIPIQQVPQEKLFSLTRANHQGVLAFASLFQYIDLQAAVDQVLSLGTAPLFILLD
                     GITDVRNVGAIARTAVCCGAQALIIPDKGIAALNEEALKSSAGALEKIHICRVSSLLK
                     AVDSLHLNGIRAYASGMQAEKKVYELDLNGPCCIMMGSEDKGLQPYLRKAADEHFTIP
                     MAGNFDSFNVSVAAGIILYEAMKKRLPLPGH"
     gene            complement(831..1391)
                     /locus_tag="VGM24_00610"
     CDS             complement(831..1391)
                     /locus_tag="VGM24_00610"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4153886.1"
                     /translation="MSNHQNPWKILDQRPVYENPWIQVTEFQVLNPSGGNGIYGKVHF
                     KNLAVGVVPLDADLNIYLVGQYRFVLNEYSWEIPEGGGTAQEDPLAAAKRELMEETGL
                     VAREWSRLLTLHLSNSVSDELAVIYLARGLEQHNSMPEETESLVVKKIPITEAVSMLE
                     SGAITDAMSVASIQQVQLRLLQGRLK"
     gene            complement(1483..2736)
                     /gene="metK"
                     /locus_tag="VGM24_00615"
     CDS             complement(1483..2736)
                     /gene="metK"
                     /locus_tag="VGM24_00615"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY4153887.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALIDHFLAFDPHSKVACETL
                     VTTGQVVLAGEVKSKAYLDVQEIARSVIRKIGYTKSEYMFEANSCGIFSAIHEQSSDI
                     NQGVVRTKKEDQGAGDQGMMFGYASNETDNYMPLALDLAHMLLIELAALRREDKQIKY
                     LRPDAKSQVTLEYDDNNNPVRIDAIVISTQHDDFDTETKMRDKIKKDIVGILIPRVRS
                     KYKKYAHLFNEQIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSGK
                     DPSKVDRSAAYATRHIAKNLVAAGVADEVLVQVSYAIGVAQPTSIHVNTFGTAKVKLS
                     DGEIGKIVEKVFDMRPYFIEQRLKLRNPIYSETAAYGHMGRKPETVVKTFVSPDGKTI
                     QKKVELFTWEKLDYVPKVKQAFRIK"
     gene            complement(2859..>3686)
                     /locus_tag="VGM24_00620"
     CDS             complement(2859..>3686)
                     /locus_tag="VGM24_00620"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY4153888.1"
                     /translation="PIPFLMVAKKRILFIANEMSPYLELTEFAETVNRLAIKSNDNGF
                     EVRCIMPRFGVINERRHRLHEVVRLSGINVSVDNDDYPLQIKVASLPNARLQVYFLEN
                     EDLFKRKFIFHDEKEKWFEDNALRTVFFCKGALETVKKFGWPPDIIHCSGWMTGLIPF
                     YLKTVYKKEPVFAHSKVVFTIGQNTFKEKLGPDFTRVANIHASIKEKDLEPFAEATNS
                     ALFRGGALFADAITFGAEKTDKKLMEEFSKVRGKKVLPFNAESDLTDYLQLYTDLSQ"
BASE COUNT          883 a          968 c          864 g          971 t
ORIGIN      
        1 gccctgcatc gcatcgcggg gacattcaac cagtttgatg ttttttttca agataaaaag
       61 tataggttaa aaatcaatgc ccggggagcg gcagccgctt cttcatggct tcgtacaaaa
      121 taatccccgc cgcaacagaa acattgaaag aatcgaaatt ccctgccatg ggaatggtaa
      181 agtgctcatc cgctgctttg cggaggtaag gctgaaggcc tttgtcttca ctacccatca
      241 tgatgcagca aggtccattc aggtccagtt catacacttt tttttctgcc tgcatgccgc
      301 tggcataagc ccggatcccg ttcaggtgca ggctatcgac tgctttcaac aaactgctca
      361 cccggcagat atgaattttt tccagagcgc cggcagatga tttcagggcc tcttcgttca
      421 gggcggcgat gcccttatcc ggaataatga gggcctgggc gccgcaacaa accgcagtcc
      481 gggcaatagc gccgacattc cgcacatcgg ttattccatc cagcaatata aacaacggcg
      541 ccgttcccag cgacagcacc tgatcgaccg ccgcctgcag atcaatatat tgaaagagcg
      601 aagcaaatgc gagaacaccc tggtgattgg cgcgggtcaa tgaaaataat ttttcctgcg
      661 gaacctgctg aatgggaata ccgcgcttct ccgccagctt ccggatctcc ccgatggatt
      721 caccgctcac gttccgctga aaaagaattt tatctattcc cctgccggac tgcatggcct
      781 cgaccagggg ctgccggcca atgattaatg aagagggttt caaagattca ttattttaat
      841 cttccctgaa gcagtcggag ctgaacctgc tggatggaag ccaccgacat cgcatcggtg
      901 atggccccgc tctccagcat gctgactgct tcagtaatag gaattttctt tacgaccagg
      961 gattccgttt cttccggcat ggaattgtgc tgctccagcc ctcttgccag gtaaataaca
     1021 gccagctcat cggaaaccga attggaaaga tgcagggtca gcagcctgct ccattcccgg
     1081 gcaaccagtc ctgtttcctc catcagttcg cgcttcgcgg cagccagcgg gtcttcctgt
     1141 gccgttccgc cgccttccgg tatctcccaa ctgtattcat tcaaaacaaa ccggtattgg
     1201 ccaaccaggt aaatattgag atccgcatcc agggggacca cgcccaccgc caggttctta
     1261 aaatgcactt tcccgtaaat cccgtttccg cccgaaggat tcaacacctg gaattccgtc
     1321 acctggatcc agggattttc ataaaccggc cgctggtcca ggatcttcca ggggttttga
     1381 tgattactca taaccgaaaa tacaaattaa aacggctgtt ccggccggaa caaaaacagg
     1441 gttaaccgga tggccaaccc tgctgatcaa tgaattcctg atttatttaa tccggaatgc
     1501 ctgttttact tttggaacat aatccagttt ctcccaggta aacagttcaa cttttttctg
     1561 aatggtcttg ccgtcggggg aaacaaatgt ttttaccacg gtctccggct tgcggcccat
     1621 atgtccgtag gcagccgttt cgctgtagat gggattgcgc agtttcaacc gctgttcgat
     1681 aaaataaggc cgcatgtcaa agaccttctc cacgatcttt ccgatttccc catccgataa
     1741 ttttactttc gccgtcccga acgtattcac gtgaatggag gtcggctgag cgaccccgat
     1801 ggcataggaa acctgcacca gcacttcatc ggctacaccc gccgcaacga ggttcttggc
     1861 aatatgccgg gtggcatagg ctgcgctacg gtccactttg gaggggtcct tcccactgaa
     1921 tgcgccgccg ccgtgtgctc cctttccccc gtaggtgtcc acgatgatct tacggccggt
     1981 aaggccggtg tccccgtgag ggccgccaat cacaaatttt ccggtcggat taatatggta
     2041 tttgatctgt tcattgaaca ggtgtgcgta ttttttatac tttgacctca cccggggaat
     2101 gaggatccct acgatgtctt tcttaatctt gtcccgcatc ttcgtttccg tgtcaaaatc
     2161 atcgtgctgg gtggaaatca cgatcgcatc gatccggacg ggattattgt tatcgtcgta
     2221 ttccagggtg acctggcttt tggcgtccgg gcgcaggtat ttgatttgtt tgtcctccct
     2281 tctcaatgcg gccagttcga tcagcagcat atgcgccaga tccagtgcca gcggcatata
     2341 attatctgtt tcattgctcg cataaccaaa catcattccc tggtccccgg ctccctgatc
     2401 ctcttttttt gtccggacaa ccccctggtt gatatctgaa gattgttcat ggatggcgga
     2461 aaagataccg caggaattgg cttcaaacat atactcactt ttggtatagc cgattttacg
     2521 gatcaccgaa cgggcaattt cctgcacatc caggtaggct tttgatttca cttctcccgc
     2581 cagcacgact tgcccggtgg tcaccagggt ttcgcaggct actttggaat gcgggtcgaa
     2641 ggcaagaaaa tggtcaatga gggcatcaga aatctggtca gccactttat caggatgacc
     2701 ttcggaaaca ctttcggaag tgaataaata agacatgtta ggctttttga aattgaacga
     2761 acaaaaataa aaaaccacta actacaacgg taattaatgg atggcggtgc aaagataata
     2821 gttgaaccat taattcattt tgttaatcgc aaagatcttt attgggaaag gtctgtgtac
     2881 aattggaggt aatcggtcag gtcggattcc gcgttgaaag gcaacacctt tttaccgcgt
     2941 accttgctga attcttccat cagctttttg tccgttttct ccgccccaaa cgtgatggca
     3001 tccgcaaaca gggcgcctcc ccggaaaaga gctgaattcg tcgcttcggc aaagggctcc
     3061 aggtccttct ccttgataga agcatgaata ttcgccaccc gggtaaagtc agggcccagt
     3121 ttttctttga atgtgttctg accgatggta aagaccacct tactgtgggc aaaaaccggt
     3181 tcctttttgt aaaccgtttt aagataaaag gggatcaggc cggtcatcca gccgctgcaa
     3241 tggataatgt ccgggggcca gccgaatttt tttacggtct ccagtgcgcc cttgcagaaa
     3301 aaaacggtgc gcagcgcgtt gtcttcaaac cacttttcct tctcgtcgtg aaatatgaat
     3361 ttgcgtttga aaaggtcctc attctccaga aaataaacct gcaaccgggc gttgggaagg
     3421 gaggccactt taatctgcag gggatagtcg tcattgtcca ccgaaacatt aatgcccgaa
     3481 agcctgacca cttcgtgcaa gcggtgcctt ctttcattaa tcactccaaa acggggcatg
     3541 atgcatcgta cttcaaaacc attgtcgttc gattttattg ctaaccggtt tactgtttcg
     3601 gcaaactcgg tcagctccag gtaaggcgac atttcattcg caataaataa aatccttttc
     3661 tttgctacca ttaggaaggg aattgg
//
LOCUS       DASYCU010000032         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3199591, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000032 DASYCU010000000
VERSION     DASYCU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3199591"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGM24_00625"
     CDS             <1..312
                     /locus_tag="VGM24_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY4153889.1"
                     /translation="HNATMHLSPEQMQGHMQKWMEWMENLKKQERFLGAQPLANTGKT
                     IAGPGKLISDGPFMESKEMVGGYLLCLANDYDEAVDISKGCPILEFEDGKVEVREVME
                     M"
     gene            314..1588
                     /locus_tag="VGM24_00630"
     CDS             314..1588
                     /locus_tag="VGM24_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4153890.1"
                     /translation="MDSANDTIYQLTDHLFRHQSGKMIAVLTRIFGLHNIELAEDVVQ
                     DAFAQALRDWTYKIPANPAAWLMTTAKNKTVDLIRKEKHKKKFAEETSALLQSEYTIV
                     PVIENLFLPDEIKDSELRMIFTCCHPSLSGPDQIALTLKTCSGFGIEEIAAALLTNRE
                     TIKKRIQRAKKMIRENDLRFVIPAGNELTKRLDTVLRSIYLLFNEGYNSSSKSSLIRK
                     DLCEEALRLALMLTENEFTDLPKSFALVSLMTLLVARFESRLDPDGEIILLEEQDRSK
                     WNDQLIRIGCNYLNRSAEGNELTEYHIEASIIAEYSTAKDFATTNWKNILLLYDRLIS
                     VNPSPIVLLNRAIVVGKISGAKAAIAEINAIPGIEKFTRSNYLFPAVLGEMYKLENNK
                     AAAIRYFEQAIALPHSEVEKRLIQRKLQQMLN"
BASE COUNT          550 a          454 c          450 g          399 t
ORIGIN      
        1 cataacgcga ccatgcatct ttctcccgag caaatgcagg ggcatatgca aaaatggatg
       61 gaatggatgg aaaatctgaa gaaacaggaa aggttcctgg gcgcacagcc attggccaat
      121 accgggaaaa ccattgccgg cccgggaaaa ctgattagcg atggtccgtt catggaaagc
      181 aaggaaatgg tgggaggcta cctcctgtgc ctcgccaatg actatgatga agcggttgac
      241 atttcgaagg gctgtcccat cctggaattt gaagatggga aagtagaggt gagggaagta
      301 atggaaatgt aaattggatt cagcaaacga tacgatttac cagcttaccg accatctttt
      361 ccggcaccag tcgggaaaga tgatcgcggt cctgacccgc atcttcggcc ttcataatat
      421 tgaactggcc gaagatgtgg tgcaggatgc gtttgcccag gcactgcgtg actggaccta
      481 taagataccg gccaacccgg cggcctggct gatgaccacg gcaaagaaca agaccgttga
      541 cctgatccgg aaagaaaaac acaagaagaa atttgccgaa gaaacatccg ctctcctgca
      601 atccgaatac acgattgtgc cggtgattga aaaccttttc ctccccgatg aaataaagga
      661 cagcgagttg cggatgatct tcacctgttg ccatccctcc ctttccggcc ccgaccagat
      721 cgcgctgacc cttaaaacct gttccggatt cggcattgag gaaattgccg ctgcgctgtt
      781 aaccaaccgg gaaaccatta aaaaaaggat ccagcgggca aaaaaaatga tccgggaaaa
      841 cgatttacgc ttcgttatcc cggccgggaa tgaattgacc aaacggctgg ataccgtttt
      901 gcgctccatc tatttattgt ttaatgaagg ctataattca agcagcaaaa gcagcctgat
      961 ccgaaaggat ctgtgtgagg aggccctgcg gcttgccctg atgcttacgg aaaacgagtt
     1021 cacggacctg cccaaaagtt ttgccctggt ttccctgatg accctgctgg tggcccgctt
     1081 tgaaagccgc ctggatccgg atggggagat catcctgctg gaagaacagg atcgcagtaa
     1141 atggaacgat cagctcatcc ggatcggctg taattacctc aaccggtcgg cagaaggaaa
     1201 tgaattgacc gagtatcata ttgaagcttc catcattgcc gaatattcca cggctaaaga
     1261 ttttgcaacc accaactgga aaaatatttt attattatac gaccggctga tcagtgtaaa
     1321 tccatccccc atcgttttgt tgaaccgggc gatcgtggtc ggcaagatct ccggagccaa
     1381 agccgccatc gccgagatca atgcaatacc cggcatagaa aagttcaccc ggtccaatta
     1441 cctttttcct gccgtactgg gggaaatgta taaactcgaa aacaataagg cggcagccat
     1501 ccggtatttt gaacaggcca tcgccctgcc ccattccgag gttgaaaaaa gactgatcca
     1561 gagaaaactg cagcaaatgc tcaattgaac acgaaggctt gctccaaaaa gatcatagag
     1621 atcctgaaaa aatcaaacag acctggaaaa aatcataaag aacccgaaat cagcatgctg
     1681 acggaacgcc ttgtcaatcc gggcgggtat gaattacacc cgggtttaat cttccttatc
     1741 ttttgaatag aaataccggg agaatgccag gaaaatggca acagcaatgg catagatcat
     1801 ggcatccgga tattgataac caaaaaatcc gatcaaaaaa actccggcca tgc
//
LOCUS       DASYCU010000033         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_734975, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000033 DASYCU010000000
VERSION     DASYCU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734975"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VGM24_00635"
     CDS             <1..614
                     /locus_tag="VGM24_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY4153891.1"
                     /translation="GYLAIAPTMRAFGDTRTEADKKEGNSFSCHTQLMHDLLLGRTVI
                     GDRVWDMSRILDWALKTLPVDPRRIAITGNSGGGTVSLFAAACDSRIRVAVPSSAFCT
                     FEGSIGTIAHCDCNYIPGILNLGEMADIAGLIAPRPLCVVNGIQDPIFPIGETRKAYT
                     QLKKIYAASGRPQNLARYEGQGGHRYYKEGVWPFIKRYFEDPN"
     gene            676..1884
                     /locus_tag="VGM24_00640"
     CDS             676..1884
                     /locus_tag="VGM24_00640"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEY4153892.1"
                     /translation="MITKLALVLSLAFSLPCQAQKFEGLALTPPMGWNSWNTFSTKID
                     EVLVKQIAEALVSSGMKKAGYTYVVLDDGWMERERDSVTGDLVADHVKFPGGMKALAD
                     YIHSLGLKFGLYNCAGTRTCAGYPGTRGYEYQDARLYASWGIDYLKFDFCHSEGINAR
                     EAYSTMSKALRKAGRPVVFSLCEWGTTQPWTWASGIGHLWRTTGDIGNVFDGIKKYRT
                     WHENGVMTIVDMQDSLRKYAGPGHWNDPDMLEVGNGMTPAEDRAHFSIWCMLAAPLIS
                     GNDLRKMSAESLAILADSGAISVDQDPDGIQAFRYPEKDSVEVWFKPLQQGAWAACFL
                     NRSSRSGKISFDWQKENVNDELNHRMLGKGAEVYKLYDVWGKKNRGTTARPLTATLAS
                     HDVLMLILRQ"
     gene            1910..3769
                     /locus_tag="VGM24_00645"
     CDS             1910..3769
                     /locus_tag="VGM24_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4153893.1"
                     /translation="MKRSFRTAQSLLLFLAVFFATTARSIAQEDSRHFSRQFNAWKPY
                     RIYLPADYSTSKKQYPVIYFFHGNQGDEKFQMDGVQQLVNEASVILVAWNGRSVPSDI
                     RPYNVGYHSNINYSIQFKDYFPEFVAYIDSAYRTLSDRRHRALIGHSMGGFMSFLLAG
                     KYPQMVCAAVSSKGSPEFFVGYPNNHTIYNTRFMFKNLYGVRVRFDNGTDGEELHNLN
                     NEVNAGAVREYGLDYSYRTYEGGHIMRPPEFRDAFNFVLDAFKNPLNEPARWQHADLY
                     PDFNVWGYQVNSDLHQPGYIDLKGVTRGGMGIRTKKWEPDGGLIPQVQIKLRTAPVYK
                     PGSDYSLFDYNVTNGVKNNSTVRSDADGRISFTVNGQYHQIGINAENTPPEIVFLTYK
                     VNDKSIFLDHRKECNLKLNLLNRGGTDAKNIKITLSSTAKGVQIGNPVITADRLDKGE
                     SKWIPADFKVIASNEVTTDGSPFRVRFNLSITDDKGNHWNDEFDAPVFYDVPEFTDIG
                     IDDGDSEIFGSGNGDNIADPGETVMIYQHSNRTRLYYDDPYIDDERLHDDLQPDKWGD
                     GYAFSSLIHISKDCPIGHKIRFLACYEVKEWKTIKRDVTWGSFTITVGKANNN"
     gene            3799..>4726
                     /locus_tag="VGM24_00650"
     CDS             3799..>4726
                     /locus_tag="VGM24_00650"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153894.1"
                     /translation="MKRKNFERILFLPILLFCLDGAARDRTTRIGADDPRIQYQGRIG
                     RANGGVAEIYWPGSSAKIKFRGTGLKAVLRDEKGYNYFNVIIDDSPVTAIRLDTTEKT
                     YVLASGLPEGTHTIELSKRADWFRGKTWFYGFETDEGAQLFRLPEKKETIEFYGNSIT
                     VGAAVEDYHGDSGDSTMTNNYRSYASITARHFKANYSCIASSGIGLMLSWGSLIMPEI
                     YDRLNPDDSLSKWDFSRAKADIVVINLFQNDCALVNLPDHPQFKRRFGDRAPSQDFII
                     NAYRKFVTDIRRHYPEAAIICVLGSMDAVKAGS"
BASE COUNT         1222 a         1164 c         1272 g         1068 t
ORIGIN      
        1 agggttacct ggccatcgcg ccgaccatgc gcgcctttgg tgatacgcga acggaggcag
       61 acaagaaaga aggcaattct ttttcctgcc atacgcagct gatgcacgac ctgctgcttg
      121 gaaggaccgt gatcggggac cgcgtttggg atatgtcgcg cattctcgac tgggccttga
      181 agaccctgcc cgttgatccg cgccggattg ccatcaccgg caattccgga gggggcaccg
      241 tttctctttt tgcggcagcc tgtgattccc gcataagggt cgccgttccc tcctcggctt
      301 tctgcacttt tgaaggcagc atcggaacga tcgcgcattg cgactgcaat tatattcccg
      361 gcatattgaa tctgggagag atggccgata ttgccggttt gattgccccg aggcctctct
      421 gcgtagtgaa cggaatacag gacccgatat tcccgatcgg agaaacgcga aaggcatata
      481 cgcaattgaa gaaaatttac gcggcttccg gtcgccctca aaatctggcc cgctatgaag
      541 gacagggagg acaccggtat tataaggagg gcgtctggcc gtttataaaa agatattttg
      601 aggatccgaa ttgagaggga tcctgttttt tacaattatc tccggtggag aaattgaaat
      661 tcctttattt tatctatgat aacaaaactc gctctggttc tttccctggc tttttccctt
      721 ccctgccagg cacagaaatt tgaaggattg gcgctcacgc ctcccatggg ctggaacagc
      781 tggaatacct tcagcacgaa gatcgatgaa gtgctggtga agcaaattgc ggaggccctt
      841 gtttcctccg gaatgaaaaa ggccgggtat acttatgtcg tcctggatga cggatggatg
      901 gagcgggaac gggatagcgt caccggagac ctggtcgccg atcacgtgaa atttccaggt
      961 ggaatgaagg ccctggctga ctatattcat tccctgggat tgaaattcgg tttgtataat
     1021 tgcgcgggaa ccaggacctg cgccgggtac ccgggcaccc gcggttatga ataccaggat
     1081 gcgcgcctct atgcttcctg gggaatcgac tacctgaaat tcgatttttg tcattcggaa
     1141 ggaataaatg cccgggaagc atacagcacc atgagcaaag cattgcgcaa ggccggtcgg
     1201 ccggtcgttt tcagtttgtg cgaatggggc accacccaac cctggacctg ggcctccggt
     1261 atcggccacc tctggcgcac caccggggac atcggaaatg tattcgacgg gataaagaaa
     1321 tacaggacct ggcatgagaa cggcgtgatg accatcgttg acatgcagga tagtttgaga
     1381 aaatacgcag ggcccggcca ttggaatgat ccggatatgc tggaagtcgg caatggcatg
     1441 acccccgcgg aagaccgcgc tcatttttcc atctggtgta tgctggccgc accgctcatc
     1501 agcgggaatg acctgagaaa aatgagtgcg gaaagtcttg ccatcctcgc tgattccggc
     1561 gccatttccg ttgatcagga tccggacggg atacaggcct tccggtatcc ggaaaaagac
     1621 agtgtggagg tttggttcaa acccttgcag cagggtgcct gggctgcgtg ttttctgaac
     1681 cgcagcagcc gctccggtaa aatttcgttt gactggcaaa aggaaaacgt gaatgatgaa
     1741 ctcaatcacc ggatgctggg aaaaggggcg gaagtttata aactatacga tgtatggggt
     1801 aaaaagaaca ggggaacgac cgccagaccg cttacggcga ccctggcttc gcacgacgtg
     1861 ctgatgctta tcctaagaca atgaattttg attgaaaaaa gtttcagcaa tgaaaagatc
     1921 cttccggaca gcgcagtcgc tgcttttatt tttggcagtc ttctttgcca cgacggcgcg
     1981 aagtattgcg caggaggaca gccgtcattt cagcaggcag ttcaacgcct ggaaaccata
     2041 tcgtatttac ctgccggcag attattccac ttcaaaaaag caatacccgg tcatttattt
     2101 tttccatggc aaccagggag acgaaaaatt tcaaatggac ggggtgcagc aactcgtgaa
     2161 tgaggcatcg gtcatcctgg tggcctggaa cgggagatcg gttccctccg acatcaggcc
     2221 gtataatgtc ggctaccatt ccaatatcaa ttactccatc cagttcaaag actattttcc
     2281 cgaattcgtc gcctacatcg acagcgctta ccggacctta agcgaccgcc ggcaccgcgc
     2341 attgatcggg cacagcatgg gcggcttcat gtcgttcctg ctggccggaa aatatccgca
     2401 aatggtttgc gccgccgtca gctcaaaggg atctccggaa tttttcgtgg gctatcccaa
     2461 caaccacacg atctacaata cccggttcat gtttaagaat ctatacggag tccgggttcg
     2521 ttttgacaac ggaacggacg gcgaggaact gcataacctg aacaatgagg tgaatgccgg
     2581 ggcggtgcgg gaatacgggc tggattatag ctaccggacc tatgaaggcg gacacatcat
     2641 gaggccgcct gaattcaggg acgcgttcaa ttttgtactc gatgcgttta aaaatccgtt
     2701 gaacgaaccg gccagatggc agcatgccga cctgtatccc gattttaatg tctggggtta
     2761 ccaggtgaac agcgacctgc atcaacccgg atatattgat ttgaaagggg taacccgggg
     2821 aggcatgggc atacggacaa aaaaatggga gcccgatggg ggactgatcc cgcaggttca
     2881 gataaagctg agaacggccc ctgtttacaa acccggctcg gactattccc tcttcgacta
     2941 taatgtaacc aatggagtca aaaataattc taccgtcaga agcgatgcgg acggacggat
     3001 cagctttacc gtaaacggac agtatcatca gatcgggatc aacgcggaga atacgccacc
     3061 cgaaatcgta tttcttacct ataaagtaaa tgacaaaagt attttcctgg atcaccggaa
     3121 agaatgcaac ctgaaactta atttattgaa ccggggaggc acggacgcaa agaatattaa
     3181 aataacgctc agcagcacgg cgaaaggggt tcagatcggg aaccccgtca tcaccgcaga
     3241 ccggctggat aagggggaat cgaaatggat cccggcagat tttaaggtga tcgcgtcaaa
     3301 cgaggtgacc accgacgggt ctccgttccg ggtccggttc aatctatcca tcaccgacga
     3361 taagggcaac cattggaatg acgaatttga cgcgcccgtg ttttacgatg ttccggaatt
     3421 tacagacatc ggcattgacg acggcgacag cgagatattt ggcagcggaa atggcgataa
     3481 tattgcagat cccggggaaa cggtcatgat ctaccagcac agcaaccgca cccggttata
     3541 ttatgacgat ccttatattg atgacgaacg cctgcacgat gatcttcagc ccgacaaatg
     3601 gggagatgga tacgcgtttt cttccctgat ccatatttca aaagattgtc ccatcggcca
     3661 taagatcagg ttcctcgcct gttatgaagt gaaagaatgg aaaacaataa aaagggatgt
     3721 tacctggggc agttttacaa ttacggtggg taaggcaaac aataactgaa caactccatg
     3781 ccgccgaaaa taaataccat gaaaagaaaa aacttcgagc ggatcctctt ccttcccata
     3841 ctcctgtttt gtctggatgg cgcggcgcgg gaccggacta cccggatagg agccgatgat
     3901 ccccgtatcc agtaccaggg aaggatcggt agggcaaatg gcggcgtcgc cgaaatatac
     3961 tggccgggct cttcggccaa aataaaattc aggggcaccg ggttgaaagc cgtcctgcgg
     4021 gacgaaaaag gatataatta ttttaacgtg atcattgacg atagtccggt tacggccatc
     4081 cggttggata cgaccgaaaa aacctatgtc cttgcctcgg gcctcccgga aggaacgcat
     4141 acgatagaac tcagcaagcg ggctgactgg tttcggggaa agacctggtt ttatggtttt
     4201 gaaacggatg aaggagcaca gctattcagg ctgccggaaa aaaaagaaac gattgaattc
     4261 tacgggaatt ccatcactgt cggtgccgcc gttgaagatt accatgggga ttccggagac
     4321 agcaccatga ccaacaacta tcgcagctat gcgtccatca ccgccaggca tttcaaggca
     4381 aactattcct gcatagcttc cagtgggatc gggctgatgc tgagttgggg ttccctgatc
     4441 atgccggaaa tttatgaccg gctgaatccc gatgacagct taagtaaatg ggatttctcg
     4501 cgcgcaaaag ccgatattgt agtcatcaat cttttccaga acgactgcgc cctggtcaac
     4561 cttcccgatc atccgcaatt taagaggcgc tttggcgacc gggcgccgtc tcaagacttt
     4621 atcatcaatg cgtaccggaa attcgtgacg gatatcaggc ggcattaccc ggaagccgct
     4681 atcatttgcg tgttgggatc catggacgcg gtaaaggccg gctctg
//
LOCUS       DASYCU010000034         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_444896, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000034 DASYCU010000000
VERSION     DASYCU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444896"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VGM24_00655"
     CDS             complement(<1..550)
                     /locus_tag="VGM24_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4153895.1"
                     /translation="MNTETAAPVVLIRSLYKTFNQEEVLKGIDLCIDKAENLVVLGKS
                     GSGKSTLIKCMIGLSEPDKGSICIMGQNIAEVNYEELNKLRIKIGFLFQSGALYDSMT
                     VYENLAFPLRHHKKHLGKQKIDELIHEVLENVGLPDAGNRMPAELSGGMSKRVGLART
                     LILKPSIMLYDEPTTGLDTATAK"
     gene            complement(547..1329)
                     /locus_tag="VGM24_00660"
     CDS             complement(547..1329)
                     /locus_tag="VGM24_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4153896.1"
                     /translation="MSTAETSLPAWVPFKRTVLHAGEFSLFFFRFLRELFKLPFEGRE
                     FLFQCYRVGNKSLPLVGVTSFIMGLVLTLQTRPSLATFGAVSLLPGMVSVSVIKEIGP
                     VITAIICAGKISSRMGAELGSMKVTEQIDAMEVSAINPFRYLVVTRVLATTFMVPLLV
                     VFADCISLLSSFLAYNIHDNISIGRFFNMAISKLDWIDIFPSLIKSVLFGTTIGIIGC
                     YKGFRVDGGTASVGTAANSAVVTASLAIFFIDLIVVQITDIL"
     gene            1496..1699
                     /locus_tag="VGM24_00665"
     CDS             1496..1699
                     /locus_tag="VGM24_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY4153897.1"
                     /translation="MTSTKLVAGVLTGLAAGAVISLLISSKKNRYWSRKLVKKGNNLA
                     DDLKGKFNEFVDRLEDKFQGILK"
     gene            1730..2071
                     /locus_tag="VGM24_00670"
     CDS             1730..2071
                     /locus_tag="VGM24_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153898.1"
                     /translation="MAETDISIKSLLEKSQDYLETKIEIVKLKTIDKSSDVLSSIVVL
                     ISVILLGLLFFLFISIGLALYLGSLVGSAYAGFFIMAGLYGILLWLLYAFREKWIKTP
                     VTNLVIKKLMK"
     gene            2078..2506
                     /locus_tag="VGM24_00675"
     CDS             2078..2506
                     /locus_tag="VGM24_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153899.1"
                     /translation="MKRSITTEEELNEAIRALEKKADLQKQELKDQFSGVVENMKPLN
                     LIKHGLQSTFSGGNKPDLLKAAIGIGSGILGRRLLVGRAGGNVLRKIITAALEFGVVG
                     LVAKNAEKLKEKGSALIEKIFHKNPHTSSDLIPDPEQPVR"
     gene            complement(2503..>3068)
                     /locus_tag="VGM24_00680"
     CDS             complement(2503..>3068)
                     /locus_tag="VGM24_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153900.1"
                     /translation="IRMNIIETIQKNLGYAIVQKIDPNSQEVTDKESIHGNHALAQAA
                     IPSVLLGLFNRLEKEPDASWLVADQPTGRLLEKIFGKSEDQVVEQIAAYSGLTGLNVK
                     TEMEHIASESVRVVRDNISDLKNENKISAFVARHKQDVLLYLPATLGMGSLLGNNNLD
                     DRTNKMEGPVSGLMHRLEKQFNSSENN"
BASE COUNT          842 a          702 c          711 g          813 t
ORIGIN      
        1 cttttgctgt tgccgtatcg agcccggtgg tgggttcgtc ataaagcata atggatggtt
       61 tcagaattaa tgtgcgggcc agaccaaccc gcttactcat tcctccggaa agctcggcgg
      121 gcattctgtt tccggcatcc gggaggccaa cattctccag cacttcatga atcaattcgt
      181 caattttttg ttttcccaga tgctttttat ggtgtctcag ggggaaggcc aggttctcat
      241 aaaccgtcat cgaatcataa agggctccgc tctgaaaaag aaaaccaatt ttaattctaa
      301 gcttatttaa ttcctcatag ttgacctcag ctatattttg tcccatgatg cagatggaac
      361 ccttgtccgg ctcactcagc ccgatcatgc atttgatcag ggtggatttt cccgatccgg
      421 attttcccaa taccaccagg ttctccgcct tatcgatgca caggtcaatt cctttgagca
      481 cttcctcttg gttgaaggtt ttatagagtg aacggatgag caccaccggc gcggctgttt
      541 ctgtgttcat aatatatcgg tgatctgtac tacaatcagg tcaataaaaa agatagccag
      601 cgatgcggtt accacggctg aatttgcggc cgtaccgaca ctggcggtac caccgtctac
      661 gcggaatcct ttataacaac cgattattcc gatcgtcgta ccgaaaagca cagatttgat
      721 cagggaagga aatatatcaa tccagtcgag cttggaaatg gccatattaa aaaaccggcc
      781 gatgctgata ttgtcgtgaa tattataggc aagaaaggag gaaaggagag aaatgcagtc
      841 ggcaaaaaca accagaagcg gtaccatgaa ggtcgtagcc agaacccggg ttaccaccag
      901 gtagcggaaa ggattgattg cggaaacctc catcgcatca atctgctcgg tcactttcat
      961 ggatcccagt tcagcaccca tgcgtgaact gattttacct gcacaaataa ttgctgtgat
     1021 tacggggcct atttctttta tgactgaaac gctcaccatt cccggcaaca gggaaacggc
     1081 accgaaggtt gccagtgacg ggcgggtttg gagcgtgagt accaacccca tgataaatga
     1141 ggttacgcct accaggggca aagatttatt tcccacccgg taacattgaa acaggaattc
     1201 cctgccttcg aatggaagct taaacagttc tctgagaaat ctaaaaaaaa acaggctgaa
     1261 ttctcctgcg tgcaaaaccg tccttttaaa aggcacccac gcaggtaaac tggtttccgc
     1321 tgtagacata taatgactgg atgtactggt tatgaggatt cagtaagaag atcgtgcctg
     1381 aagtttaccg ggcagttaat aggtaacccg gctgttttgc aggaggcatc gtagaaataa
     1441 ctacacagca atccggcctg atttttatgc tatatatttt ataaaatcga actatatgac
     1501 aagcacaaaa ctggtagcag gtgttttaac cggactggct gcgggagccg taatcagcct
     1561 cctgatatct tctaaaaaga atcgctattg gagccggaaa ctggtgaaga aaggaaataa
     1621 cctggctgat gatctgaaag gtaaattcaa tgaatttgtg gaccggcttg aagataaatt
     1681 ccagggtatt ttaaagtaac taactttgcg gcaagcatca actattaata tggcggaaac
     1741 agatatatcc attaaatcgc tgctcgagaa atcacaggat tatttagaaa cgaaaattga
     1801 aatagtaaaa ctgaagacga tcgacaaatc gtcggacgtg ctgtccagca tcgttgttct
     1861 gatatccgtg atcctgctcg gccttctgtt tttcctgttt atcagcattg gtcttgcact
     1921 ttatctcggt tccctcgtgg gctccgccta tgccgggttt tttattatgg ccgggctgta
     1981 tgggatcctg ctttggcttc tctacgcctt ccgcgaaaaa tggatcaaaa ccccggttac
     2041 caatctggtc attaaaaaat tgatgaaata gtcagctatg aagagatcta tcaccacgga
     2101 agaggaactg aacgaggcaa ttcgggcatt ggagaaaaaa gcggatttac aaaaacagga
     2161 gttaaaggat cagttttccg gtgtagtgga aaatatgaag ccgctcaacc tgatcaagca
     2221 cgggcttcag tccacttttt cgggagggaa caaaccagat ctcctgaagg cggccatcgg
     2281 aattggctcc ggaatactgg gcagaagatt gctggttgga agagccgggg gaaatgtgct
     2341 tcgaaaaatc atcactgctg cccttgaatt cggcgtcgtg ggcctcgtag caaagaatgc
     2401 ggaaaaatta aaagaaaaag ggtcggcgct gattgagaaa atttttcata aaaaccccca
     2461 taccagttcc gaccttatac ctgatcctga acagcctgtc cgttaattat tttctgaaga
     2521 attgaattgt ttttccaggc gatgcatcag gcccgacacc gggccttcca ttttattggt
     2581 tctgtcatcc agattattat tgcccagcaa actgcccatt ccgagagtcg ccggcagata
     2641 aagcagtacg tcctgcttgt gccgggccac gaatgcgctt attttgtttt cgtttttcag
     2701 gtcagaaata ttatcccgga ccacccggac ggattcagac gcaatatgct ccatttcagt
     2761 ttttacattc aaaccggtta agccggaata ggccgctatc tgttcaacca cctgatcttc
     2821 tgacttaccg aaaatctttt ccaacagtct tccggtgggt tggtcagcga cgagccagga
     2881 agcatccggc tccttttcca gcctgttaaa aaggccaagc aatacagaag gaatcgccgc
     2941 ctgtgccaac gcatgattgc cgtgaatgct ctccttatcc gttacttcct gggaattggg
     3001 gtcgatcttc tggacgatcg cataacccag atttttttga atggtttcta taatattcat
     3061 acgtatgc
//
LOCUS       DASYCU010000035         6332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1460157, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000035 DASYCU010000000
VERSION     DASYCU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460157"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1092)
                     /gene="sucD"
                     /locus_tag="VGM24_00685"
     CDS             complement(220..1092)
                     /gene="sucD"
                     /locus_tag="VGM24_00685"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY4153901.1"
                     /translation="MAVLVNKGSKIIVQGFTGTEGSFHASQMIEYGTKVVAGVTPGKG
                     GSKHLDRPVFNTVAAAVEETGADVSIIFVPPAFAADAIMEAAEAGIPLVVCITEGIPV
                     QDMVKVKNYLVGKNTRLIGPNCPGVITADECKVGIMPGFVFKKGKIGIVSKSGTLTYE
                     AADQVAKAGLGISTAIGIGGDPIIGTTTREAVELLMNDPETEGIVMIGEIGGNMEAEA
                     ARWVKKNNSKPVVGFIAGQTAPPGRRMGHAGAIIGGAEDTAAAKMKILGECGIHVVQS
                     PADIGATMAKVIKK"
     gene            complement(1134..1943)
                     /locus_tag="VGM24_00690"
     CDS             complement(1134..1943)
                     /locus_tag="VGM24_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4153902.1"
                     /translation="MIEIKDLKKSFDGKTVIDGVSAVMQTGKCNLIIGSSGSGKTVFM
                     KCLVGLFEPDSGEISYGGQSFTAMNEEERKTLRQQIGMLFQGSALFDSMTVEQNIMFP
                     LDMFTKDSFSAKQKRVNEVLARVNLEDVNNKYPAEISGGMKKRVGIARAIVLNPKYLF
                     CDEPNSGLDPQTSLLIDKLIQEITREYNITTVVNTHDMNSVMETGDHIIYMYQGLKEW
                     EGSNKEIIFSKSQKLNEFIFASEFLRDAKDMRMLEMKGKIDNDRNMDELLK"
     gene            complement(1956..2693)
                     /locus_tag="VGM24_00695"
     CDS             complement(1956..2693)
                     /locus_tag="VGM24_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4153903.1"
                     /translation="MNIFTEFGRYLLMLKDMFSRPENFKMYWKELMHQCVEIGIRSLG
                     IVLIISFFMGAVSALQTAYQLVSPLLPRSTIAMIVRDTVILEFSPTLVCIVLAGVVGS
                     KIAGELGNMRVSEQIDALEIMGINSRTFLVLPKILAALITIPLLVVISMVLGIWGGRL
                     ASVATGIVAASQFDKGLLQDFLPYNVFFALAKAYTFSFIISSISSFYGYYVSGGSLEI
                     GRASTKSVIVSCVLILLADYVLSAVLL"
     gene            complement(2721..5051)
                     /locus_tag="VGM24_00700"
     CDS             complement(2721..5051)
                     /locus_tag="VGM24_00700"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY4153904.1"
                     /translation="MQKKQLRKEEALDYHSKGRAGKIEVIPTKEAKTQRDLSLAYSPG
                     VAEPCKEIINNAEDVYKYTAKGNLVAVITNGTAVLGLGDIGPEASKPVMEGKGVLFKI
                     FADIDVFDIEINEKDPEKFVQIVKALEPTFGGINLEDIKAPECFYIEKELRSALRIPV
                     MHDDQHGTAIISSAALINALELQRKKIDRVRFVVNGAGAAAMACIKLYVALGAKPANF
                     TVFDKDGVIHKDRPDLDGDRLQFAVDKPDQTLEKAMKDADVFVGLSIGNTVTPEMVKS
                     MARKPIVFAMANPDPEIDYEMATAVRDDLIMATGRSDYPNQVNNVLGFPFIFRGALDV
                     RATQINEAMKFAAVYALAGLAKTPVPDIVNMAYNEKNISFGPLYIIPKPLDPRLLSTV
                     APAVAKAAIESNVAQTRIENWDAYAIELNKRLGLDNQLIRVLGSKARRDPRRLVFAEA
                     DNLKILKVAQILFDEGVAYPILLGNKARIQAIAGHNKIDIEELPIVDPRSEEERERRK
                     NYSEIFFQKRQRRGFNKYESQKIMRDRNYFGCMMVETGEADAMISGLTKNYPDTIRPA
                     IEIIGTEENVKKIAGMYLLLTKKGPLFLADTTVNFNPTAEELADITLLVAKEVRHFNL
                     VPRIAMLSYSNFGTSNSPEARLVARATEMVKSKAPDLIVDGEMQASIALNNEILRENY
                     PFSSLVDQEVNTLIFPNLAAGNVAYNLLKEVGGADAIGPILLGMKRPVHILQLGSSVR
                     NIYNMALVAVIDAQIKCPSTPAETLRQSAGWKRTKK"
     gene            complement(5060..6244)
                     /locus_tag="VGM24_00705"
     CDS             complement(5060..6244)
                     /locus_tag="VGM24_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY4153905.1"
                     /translation="MLKEKIHQLAKQYAPETIDIRRHLHAHPELSYKEFQTSAFVQEK
                     LKDLGISFEIKAATGVVGMIKGKNPGKRVIALRADMDALPIREENEVPYKSQNDGVMH
                     ACGHDAHTSILLGASKILNELRDEWEGTVKLIFQPGEEKNPGGASLLIKEGVLHNPQP
                     QAIFGLHVHPGLEIGKLSFRSGPSMASADEIYITVSSKGGHAATPHLTVDTILVASQL
                     VVSLQQIISRNKNPFTPSVLSISSFQGGFTTNVIPSEVKLMGTFRAMDETWRFQAHNL
                     IRKMAKGLALSTGATIDVNVDVGYPVVNNDVQLYPVARTRAEEYAGKENILETEVRMG
                     AEDFGYYTQEIPGCFYRLGVMNEAKGITSSVHTPTFNIDESAIETGMGMMAWLGASVY
                     SS"
BASE COUNT         1432 a         1739 c         1484 g         1677 t
ORIGIN      
        1 cacgaaatgg catatgagcg caacagggtt tcccggggtc atcgggaaat gagaatggat
       61 aaacgcgaca tccggcacga ccggaaacaa ttcaaacggc atcaccggca tcaccgcggt
      121 cacagacgcc atcgcgtttc atagcattgg acaatttatt tccgtagaaa ttccggtata
      181 aaccggtaaa gcaaaaaccc ggctcggtgc cgggtttttt tattttttaa tcaccttggc
      241 cattgtcgca ccaatatccg caggactctg gaccacatga attccgcatt cccccaggat
      301 cttcattttt gccgcggcgg tatcttctgc gccgccgatg atggctcctg catgacccat
      361 ccggcggccc ggaggagcgg tttgcccggc aataaaccca acgacgggct tgctgttatt
      421 ttttttgacc catcgcgccg cctctgcttc catatttccg ccgatctctc cgatcatgac
      481 aattccttcg gtttccggat cgttcatcag caattccacg gcttcgcggg tggtggtccc
      541 gatgatggga tccccgccga tgccaatggc cgtcgaaatg cccagaccgg ccttggccac
      601 ctggtccgcc gcttcataag taagggttcc ggatttggaa acgataccga tctttccttt
      661 tttaaataca aagccgggca tgatgcccac cttgcattcg tctgccgtga taacgccagg
      721 gcagttgggg ccgatcagcc gggtattctt gcccaccagg taatttttta ctttcaccat
      781 atcctgcacc ggtattccct ccgtgatgca aaccaccagc gggattccgg cttccgccgc
      841 ctccataatg gcgtctgcgg caaaagcagg cggcacaaaa ataatggata catcagcgcc
      901 ggtctcttcc accgcagcgg caaccgtatt gaacacgggc cggtccaggt gtttgctgcc
      961 tcccttgccc ggtgtaactc cggcgaccac tttcgttcca tattcaatca tctgtgaagc
     1021 gtgaaaactt ccttctgttc ctgtaaatcc ctgaacgatg atttttgatc ctttatttac
     1081 taagactgcc attgatttgt gtttaggtct ttctgtttgt gccgcaaaat tagctatttc
     1141 agcaattcat ccatgttccg gtcattatca attttgcctt tcatttcgag catgcgcata
     1201 tcctttgcgt cccggaggaa ttcagaggca aaaataaact cgtttaattt ttgacttttg
     1261 ctgaaaatga tttccttatt actgccctcc cattctttca ggccctggta catgtagatg
     1321 atgtgatcgc cggtttccat cacgctgttc atatcgtgcg tattgacgac ggtggtgata
     1381 ttatactccc tggtgatctc ctggatgagt ttatcgatga gcagggaagt ctgcggatca
     1441 agcccggaat tcggttcatc acaaaaaagg tatttcggat tgagtacgat agcccgggca
     1501 atgcccacgc gttttttcat ccccccggaa atttccgccg ggtatttatt atttacgtct
     1561 tcgaggttca cccgggccag cacttcgtta acgcgttttt gtttggcaga aaaggaatcc
     1621 ttggtgaaca tgtccagcgg gaacatgatg ttctgctcca ccgtcatgct atcgaatagg
     1681 gccgaaccct gaaacagcat cccgatctgc tgcctcagcg tctttctttc ctcttcattc
     1741 atggccgtaa aactttgccc gccatagctg atctccccgg aatcgggttc aaaaaggccc
     1801 accaggcatt tcatgaagac cgttttgccg cttccgctgg aaccgataat caggttgcat
     1861 tttccggtct gcataaccgc actgacgccg tcaataactg ttttgccgtc aaatgatttt
     1921 tttaaatctt ttatttcgat catagtggat aatacttaca ataaaacagc ggataaaaca
     1981 taatctgcca acaggatgag cacgcaactc acgataaccg atttggtgct ggcccttccg
     2041 atctccagtg atccgccgct cacgtagtat ccgtaaaagg aagagatgct ggaaataata
     2101 aatgaaaatg tatacgcttt tgccagcgca aaaaacacat tgtaaggaag gaaatcctgt
     2161 aataatcctt tatcgaactg ggaagccgcc acgattccgg tggccacgct ggccagtctt
     2221 cctccccaga tccccaatac catggaaata accaccagca ggggaatggt gataagcgcc
     2281 gccaggattt tcggcagcac gagaaaggtc cggctattga tccccatgat ttccagggca
     2341 tcaatctgtt cgctgacccg catattaccc agttcgccgg cgatcttgct gcccaccacg
     2401 ccggccagca caatacagac cagggtggga gaaaattcga ggatcaccgt gtcccggacg
     2461 atcatggcaa tcgtggacct gggcaggagc ggactcacca gctggtaagc ggtttgtaat
     2521 gcagaaacgg cccccataaa aaaggagatg atcagcacga ttcccaacga gcggatgccg
     2581 atttcaacac actgatgcat gagttccttc cagtacatct tgaagttctc cggcctggaa
     2641 aacatatcct tcagcatcag taaataccgc ccgaattctg taaatatatt catgaatgat
     2701 tactggaatg aaagtacaat ttattttttg gttcgtttcc aacccgcgga ttggcgcagg
     2761 gtttcagcgg gggtggacgg gcatttgatc tgcgcatcga tgacggccac cagggccata
     2821 ttgtaaatat tccgcacgga actgcccagc tgaagaatat gcaccggcct tttcattccc
     2881 aataaaatcg ggccgatcgc atctgccccg cccacttctt tcaacaggtt ataggccaca
     2941 ttgcctgcgg ccaggttggg aaaaataagc gtattcactt cctggtccac cagcgaactg
     3001 aaaggataat tttcccggag aatttcatta ttcaaggcta tgctggcctg catttcccca
     3061 tccacgatca ggtccggcgc tttactcttc accatttcgg tggccctggc caccagccgg
     3121 gcttccggag aattactggt tccgaaattg gaataactca gcatcgcaat gcggggaacc
     3181 agattaaaat gcctgacttc cttggccacc agcaaggtga tgtcggccag ctcttctgcc
     3241 gtgggattaa aattcacggt ggtatccgca agaaaaaggg gccctttttt ggtgagcaaa
     3301 agatacatgc cggcaatctt tttcacgttc tcttcggtac cgatgatttc aatggccggc
     3361 cggatggtgt ccggataatt cttggtgagg ccggagatca tcgcgtcggc ctcccccgtt
     3421 tccaccatca tacaaccgaa atagttccgg tcgcgcatga tcttctggga ctcatatttg
     3481 ttaaatccgc gccgttggcg cttttggaag aaaatttccg aataattctt tctccgctcc
     3541 cgctcctcct ccgacctcgg atccacgatc ggcaattctt caatatcaat tttattatgc
     3601 ccggcgatgg cctgtatcct ggctttattc cccagcagaa tgggataggc cacgccctca
     3661 tcaaataata tctgggcaac tttcaggatc ttgagattgt cggcttcggc aaataccagc
     3721 cgcctcgggt cgcgcctggc cttacttccc aatacccgga tcaactgatt gtccagcccc
     3781 agccttttgt tcagttctat ggcataggca tcccagtttt caatccgggt ctgggctaca
     3841 ttgctttcga tggctgcttt ggcgactgcc ggcgctaccg tggaaagcag gcgcggatcc
     3901 agcggcttgg gtatgatata taaaggtccg aaactgatat ttttttcatt gtaagccatg
     3961 ttcacgatat ccgggacagg cgtctttgcc aggccggcca gtgcatacac ggcagcgaat
     4021 ttcatggctt cgtttatctg ggtggctcgc acatccagcg ccccccggaa aataaaagga
     4081 aaaccaagca cattattcac ctggttggga tagtcactcc ggccagtggc catgatgagg
     4141 tcatcgcgga ccgcagtggc catttcataa tcaatctccg ggtccggatt ggccatggcg
     4201 aaaacaatcg gtttcctggc catacttttc accatttccg gggtgacggt gtttccaatg
     4261 cttaaaccga caaaaacatc cgcgtctttc atcgcttttt caagcgtctg atccggttta
     4321 tccaccgcga actgaagcct gtccccgtcc agatccggcc tgtctttgtg aatgaccccg
     4381 tccttgtcaa acaccgtgaa atttgccggc tttgccccga gcgctacgta tagtttgata
     4441 caggccattg ctgccgcccc tgcgccattg accacaaacc tgacccggtc aatttttttt
     4501 ctctgcagtt ccagcgcatt gatgagggcg gcgctgctga ttatggccgt gccgtgctgg
     4561 tcatcatgca ttaccggtat ccggagcgca gaacggagtt ctttctcgat ataaaagcat
     4621 tcaggcgcct tgatgtcttc caggttgatt cctccgaaag tgggttccag tgcttttacg
     4681 atctgcacaa atttctccgg gtccttttcg ttgatttcaa tatcaaatac atcgatatcc
     4741 gcaaaaattt tgaacagcac ccctttccct tccatcacgg gttttgaagc ctcgggaccg
     4801 atatctccca gccccagcac cgccgtgcca ttggtgatca cggcaaccag atttcctttg
     4861 gcggtatatt tataaacgtc ttccgcattg tttatgattt ccttgcaggg ctcggccacc
     4921 cccggcgaat acgccaggga aagatcgcgc tgggtcttag cttccttggt gggaatcacc
     4981 tcaattttac cggcccttcc tttggagtgg taatcgagcg cttcctcctt cctgagttgc
     5041 tttttttgca tggaataagt taagaagaat aaacacttgc tcccaaccag gccatcattc
     5101 ccatgccggt ctcaatggcg gattcatcaa tattaaaagt aggcgtatgt acgctggaag
     5161 ttattccctt tgcttcattc atcaccccga gccgataaaa acatcccggt atttcctggg
     5221 tgtagtatcc gaaatcctct gcccccatac gcacttctgt ttccagaata ttttccttac
     5281 cggcatattc ttccgccctt gtcctggcca ccggatagag ctggacatcg ttattcacca
     5341 ccggataacc cacatccacg ttaacgtcaa tcgtggcgcc ggtggacaag gccagcccct
     5401 ttgccatttt ccggatcagg ttatgggcct gaaaccgcca ggtttcatcc atggcgcgga
     5461 aagtgcccat cagctttacc tccgatggga tcacattggt ggtgaagccg ccctgaaaag
     5521 agctgatgga gagcacggat ggggtaaacg gattcttatt ccggctgatg atctgctgca
     5581 gggaaaccac cagctgggaa gccaccagga tcgtatccac ggtgagatgc ggggtggccg
     5641 catgtcctcc tttactgctc accgttatat aaatttcatc ggcactggcc atggatggtc
     5701 cgctgcggaa actcagttta ccaatctcca ggcccgggtg cacgtgcagt ccgaagatgg
     5761 cctgcggctg cgggttgtgc aggaccccct ctttgatcag gagactggcg ccgccggggt
     5821 ttttttcttc tcccggctga aagatcagct tcaccgttcc ttcccattca tccctcaatt
     5881 cattcagaat tttagaggcg cccagcagga tactggtatg cgcatcgtgt ccacaggcat
     5941 gcattacccc atcattctgc gatttgtaag gaacttcatt ttcctcccgg atcggcaggg
     6001 catccatatc ggcccgcagg gcaatcaccc gtttaccggg attctttccc ttgatcattc
     6061 ccaccacccc cgtggcagcc ttgatctcaa aagaaatgcc caggtctttc aatttctcct
     6121 gcacgaaagc ggaggtttga aattctttgt agctcagctc cggatgggca tgcaggtgac
     6181 ggcggatatc aatggtttcg ggtgcatatt gtttggccag ttgatggatc ttttctttca
     6241 gcataagtat aaattactca ggtttcactt caatgatatc ggggaccagg taaacgccgg
     6301 acgggaaaaa tttggtgagc ttgtcgatat ag
//
LOCUS       DASYCU010000036        10481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4215022, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000036 DASYCU010000000
VERSION     DASYCU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4215022"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VGM24_00710"
     CDS             <1..962
                     /locus_tag="VGM24_00710"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEY4153906.1"
                     /translation="FDAIEALPPATHPMTQFVIAIMALQTESQFAKRYAEGLSKKDYW
                     EAIFDDSMNLIARLPRVAAYIYRRKYKNGDHIQPDGLLDWAGNFAHMLGYQNDTFQEL
                     MRLYMTIHADHEGGNVSAHTTHLVGSALSDPYLSLAAGMNGLAGPLHGLANQEVIRWI
                     MDMRKELNTELPSKEQIVEYVQKTLNEGKVVPGYGHAVLRKTDPRFTAQMEFGKKHIP
                     NDPLVKTVWNIYEAVPPVLQSLGKIKNPWPNVDAHSGALLVHFGLVEYEFYTVLFGVS
                     RSLGVLASLCWDRALSYPIERPKSVTTESVKAWLEGHGEIWGD"
     gene            complement(983..2149)
                     /locus_tag="VGM24_00715"
     CDS             complement(983..2149)
                     /locus_tag="VGM24_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY4153907.1"
                     /translation="MALHPVSKLIRTRAKQTGEMEHSSPLFLTSSFCFDNAEEMRAAF
                     ADENDDNIYSRFSNPTVQEFTDRMCVLEGMESGFATSSGMSAVFACFLSFLKQGDHLL
                     SCRSIFGSTHSVITKFLPRYGIEHSYVSAGDGSDWEAAIRPNTRMIFVETPTNPGLDV
                     IDLEAAGRLAKKHNLIFCVDNCFATPIGQLPARFGADLVVHSATKWLDGQGRVLGGIV
                     LGNKKLIHDVYLFCRSTGPALSPFNAWVLSRSLETLEVRMERHVSNAFYLAQELQKHP
                     AVSWLKYPFLESHPQYAIARKQMLNGGGLLCFELKGGLASGIKFLDKLHMLSRTANLG
                     DTRSIASHPASTTHGKLSEEERKAVNITPGLIRISAGLEHREDILEDIVQAMEG"
     gene            complement(2198..2620)
                     /locus_tag="VGM24_00720"
     CDS             complement(2198..2620)
                     /locus_tag="VGM24_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY4153908.1"
                     /translation="MAHVEVTRVSNDFGFEGTDATGHRVKMDSSPESGGHDSGIRPMQ
                     MLLIGLGGCSGIDIVSILKKQKQTVEGFRISIDGEREQGKEATRWKQIQIVFELKGNI
                     DPEKAKRACALSIDKYCSVAATLVGAGCRIQWECHISS"
     gene            2835..3878
                     /gene="metX"
                     /locus_tag="VGM24_00725"
     CDS             2835..3878
                     /gene="metX"
                     /locus_tag="VGM24_00725"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY4153909.1"
                     /translation="MEEFHIDEPFQLENGQSLSGIKIAYHTYGNLNDNADNVVWICHA
                     LTANSNVTRWWPGMVGENHIINPQTHFIVCANILGSCYGTSGPLSEDPGTGKIYFSHF
                     PAITIRDMVQAHILLRKQLGIRRIALLMGGSMGGYQALEWCVMEPEIIRDLFLIATSA
                     AETAWGIAVHTAQRLAIEADPGWKDLNEDAGSGGLKAARAIGILTYRNYMIMKEKQAD
                     PDPEKLDHFRASSYINYQGDKLVERFNAYSYWLLTKAMDTHNLSRGRGGDLKKVLSSM
                     RQRTLIIGITSDILCPLDEQKFISDYIPDCKMISIDSIYGHDGFMVESDKIADGLKKW
                     INLTSSVFPDNNP"
     gene            complement(3844..5283)
                     /locus_tag="VGM24_00730"
     CDS             complement(3844..5283)
                     /locus_tag="VGM24_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4153910.1"
                     /translation="MSTTMKWFRIWILSAAMAFHHTGYCQDSTATTLNLKQCVELAIQ
                     NNLQVKQSEIQMKTSGVQFKQAKDNLLPQINASGSQGLNYGRSINNLNNTYVDQQNNS
                     GNYGVNASLVLFSGLQNQNAIRQNALNFDAGKMDWQQQKDNITLNVILGYLLVLSNQD
                     QLTIARQQATVDSLQVVRLEIQNRAGAIAPATLYDLQGQYANDRVNLVNAINNLETSK
                     VNLFGLLNIPYQKEATYEAVPLDANTLQYGGSSDSIFQSALQTIPLIKAADLRVRSFE
                     KAVAVARGKLYPTLLFYGNINSNYTNIATTGIPGTEFKTSTGQFVDIGASSYDVQSFS
                     VQQRKISFGDQFKNNRYESIGLQLNIPILNYLSTRNNIKQAKINLENARVLAGASRNQ
                     LQQQVEQAWQNMNSAYGQYQGYLDQVKAYGESFRTAEIRFNNGVITSVDYVIAKNNYD
                     RANTNLTAARYNYIFRSKIMDYYQGRLSW"
     gene            complement(5280..5960)
                     /locus_tag="VGM24_00735"
     CDS             complement(5280..5960)
                     /locus_tag="VGM24_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4153911.1"
                     /translation="MIKLEHISKWVNIGNIRNFILKDINLQIGEGEFISVMGPSGSGK
                     STLLNIIGMLDTPSEGDYQFMDTDVYKLKEKQRSNLYKTHIGFVFQAYHLIDELTVYE
                     NIETPLIYQNIKTSERKAIVADILDRFQMVGKKDLFPTHLSGGQQQLVGIARSLVAKP
                     KLILADEPTGNLNSKQGEEIMELFTKLNQQDKVTIIQVTHSEKNAGYGKRIVHLLDGR
                     IEKTETNK"
     gene            complement(5985..8366)
                     /locus_tag="VGM24_00740"
     CDS             complement(5985..8366)
                     /locus_tag="VGM24_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4153912.1"
                     /translation="MLKNYFKIAFRNLWRHRVFSFINIMGLTVGMTACFLIFLYVNFE
                     LSYDSFHTKADRIYRVVTDLKTPSETIHTSGPSWAVGPHIMQDFPQVEAAVRTTGDEL
                     LVRKGNIKFQETNSLYADSLFFKVFDFKLLKGNPNTALNEPFSLVLSETAVKKYFGNA
                     DPVGQTVLFTGGGLPAKITGVMKDFPENSQIKADLLVSMSTLTRNFNKGLDDQWGNYG
                     NSTYVLLKPGTNAKALQAQLPAFLERRNGSEMKQMNMYPTIILEPLKKVYLYSTRNGS
                     NTGNINNVYIFSIVAAFLLLIACFNFVNLTTARSAERAKEVGIRKVVGAAKTQLTWQF
                     IGESVLLCLIAFLFTLGATTLLLHSFNQLAGKTISSGIFENMRFILLLFLAAVGIGLL
                     AGFYPALVLSSFRPVTVLKGRFSTGTRGVLLRKGLVVTQFSINIGLIIATIIVYKQLN
                     YMRNQDLGFKKNQMLVINTNFDPARESFRNAVASLPNVLSVAMSSSVPGGGNPGAYSE
                     IENKKGEMQIANLDLYFVDFDYIPQYKIKMVAGRPFSRDFQTDTTQAMILNEAAVRLF
                     GYSSPQQAIGQNFSQWGRKGKIIGVVKDFHFRSLQQEISPLSMRIEPQGCNLVSINLT
                     TQNLPKTIAAIESKWNALIPARPFSYFFLDEFFDKQYRSEERFGKLFVNFAVLAIFIS
                     CLGLLGLASYSTLQRTREIGIRKVLGASVSNIVNLLSRDFIKLVALAIVIASPVAWWA
                     MHNWLKDFAYQIKIGWWVFFVAALLAVLIALITVSFQAIKAAVANPVNSLRSE"
     gene            complement(8368..9045)
                     /locus_tag="VGM24_00745"
     CDS             complement(8368..9045)
                     /locus_tag="VGM24_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4153913.1"
                     /translation="MILIHDLEKIYKTEEVETIALNKLSFQVNDGEFVAVMGPSGCGK
                     STLLNILGLLDDPDNGSFMFNGIEVAHFNERKRADLRKRNIGFVFQSFNLIDELTVFE
                     NVELPLIYLGVKGEERKKRVEEMLDKMQIMHRRNHYPQQLSGGQQQRVAVARAVVNKP
                     KLILADEPTGNLDSANGNEVMELLTDLNEQGTTIIMVTHSEHDARYSHRIIRMLDGQT
                     VTENILA"
     gene            complement(9076..10332)
                     /locus_tag="VGM24_00750"
     CDS             complement(9076..10332)
                     /locus_tag="VGM24_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY4153914.1"
                     /translation="MVDRVIKKKKWPLKKVLTILGIVALVLLAFGSYYFTAGKSRLNV
                     ELDRITISTVTKGPFQETIPVNGVVLPITSIYLDASEGGRVEEKYVEDGAFVKKGQPI
                     LRLSNTDIQLALVTQQTNVFNLLTQMQISRNAAQQNTVNKRNQLTDVESQLREAKRIY
                     DLDKTLYAQKAIGSQEYLKAENDYDYYLQKKKLTDQIMQQDSVSTEQQLKSDQQSFKG
                     SQQALNVMHEKVGDLIVRARVDGQLTSLDAEVGQNKNKGERLGQVDVLSGFKVRVDVD
                     EHYLSRIYTGLKGKFSFADSNYDLKIFKVYSQITAGHFQVDMQFLGPVPKGLRRGQTL
                     QILLALSDERQSVLVPRGGFFQQTGGNWIFKVSEDGKSAYRVDIQLGNQNQDYYEVLQ
                     GLKPGDKVVTSSYETYGTMQELIFDK"
BASE COUNT         2499 a         2652 c         2576 g         2754 t
ORIGIN      
        1 tatttgatgc cattgaagcc ctcccgccgg ccacccatcc catgacgcag ttcgtgatcg
       61 ccatcatggc acttcaaacg gaaagccagt ttgccaaaag atatgcggag ggacttagca
      121 aaaaagatta ctgggaagcc atattcgacg attccatgaa cctgattgcg cggcttcctc
      181 gggtcgctgc ctatatctac cgtagaaaat ataaaaacgg agatcatatt cagccggacg
      241 gactgcttga ctgggccggc aattttgccc acatgctggg ttaccagaat gatactttcc
      301 aggaattgat gcgtttgtat atgaccatcc atgccgacca tgaaggaggc aatgtgagcg
      361 cgcataccac ccacctggtc gggtcagccc tcagcgatcc gtatctctcc ctggctgccg
      421 gcatgaacgg actggctggc ccgctgcacg gtctcgccaa tcaggaggtg atccggtgga
      481 tcatggatat gcgcaaagaa ctgaatacgg aacttcccag caaggaacag atcgtggaat
      541 atgttcagaa aaccctgaat gaaggtaaag ttgttcccgg atacgggcat gccgtactcc
      601 gtaaaacgga tccgcgcttc accgcccaga tggaattcgg gaaaaaacat atacccaacg
      661 atccgctggt aaaaaccgtt tggaatattt acgaggcggt gcctccggta ttgcaatctc
      721 tggggaaaat aaaaaatccg tggccgaatg tggatgcgca cagcggtgcc ctgctggttc
      781 attttggctt ggtggaatat gaattttata ccgttctttt tggcgtaagc cggtctctcg
      841 gcgtgctcgc cagcctttgc tgggaccggg cgctgagtta tccgatcgaa aggccgaaat
      901 ccgttaccac cgaatccgtg aaagcctggc tggaagggca tggcgaaatt tggggggact
      961 agctcctggt ccggccggct catcagcctt ccattgcctg aacaatatcc tccaggatgt
     1021 cctcccggtg ttcgagcccg gcagaaatgc gtataagccc aggcgttata tttaccgcct
     1081 ttctttcctc ttcacttaat tttccatgcg tcgtgctggc cggatgggaa gctatgctgc
     1141 gggtatctcc caggttggcg gtgcgggaga gcatgtgcag tttatccagg aattttatgc
     1201 cgctggccag tccgcctttc aattcaaagc ataaaagacc accgccattc agcatttgtt
     1261 tcctggcgat ggcatactgg ggatggcttt cgagaaaagg atattttagc cagctgacgg
     1321 ccggatgttt ttgcagttcc tgagccaggt aaaaggcatt ggatacatga cgctccattc
     1381 tcacttccag ggtttccagg ctccggctga gcacccaggc attgaaaggg gatagtgcgg
     1441 gccctgtact tcggcaaaaa agatagacat catgaatcag ctttttattg cccagcacga
     1501 tgccccccag aacacgtcct tgtccgtcca gccattttgt ggccgaatga acgaccaggt
     1561 cggccccaaa ccgcgccggg agctgcccga tgggcgtggc aaaacaatta tccacacaaa
     1621 agatcaggtt atgttttttt gccagcctgc cggcggcctc caggtcgatc acgtcgagcc
     1681 ccgggttggt gggcgtttcc acaaaaatca tccgggtgtt gggccggatg gccgcttccc
     1741 agtcactccc gtcgccggcg gatacataac tgtgttcaat gccgtatctg ggaagaaact
     1801 tggtaatcac actatgggtg cttccgaaaa tagatctgca ggataacagg tgatcgccct
     1861 gcttcaaaaa gctaagaaaa caggcaaata cggcactcat ccccgaggaa gtggcaaacc
     1921 cggattccat cccttccagc acacacatgc ggtcggtgaa ttcctgaacg gtggggtttg
     1981 aaaaacggct gtagatattg tcgtcatttt catcggcaaa tgcagcccgc atttcctccg
     2041 cgttgtcgaa acaaaaactg ctggtcagga agaggggaga agaatgttcc atttccccgg
     2101 tttgttttgc acgggttctg atgagtttgg aaacagggtg taatgccatg atcggatttt
     2161 taatatatga ttttgggtgg cggggaccat ttcccgctca ggaactgata tggcattccc
     2221 attgaatgcg gcatcccgcg cctaccaaag tggctgctac ggagcaatat ttgtcaatgg
     2281 aaagggcgca ggcccttttc gctttttcag gatctatatt cccctttaat tcaaagacga
     2341 tttggatttg tttccatcgg gtggcctctt ttccctgttc tctttctccg tcgatgctga
     2401 tccggaatcc ctctacggtt tgcttttgtt ttttgagaat ggaaacgata tcaatgccgc
     2461 tgcatccccc caggccgatc aataacatct gcatcggccg gatgccggaa tcatggcccc
     2521 cgctctccgg gctggagtcc atcttcaccc ggtgaccggt ggcgtcggtt ccttcaaatc
     2581 cgaaatcatt actaacgcgg gtgacttcaa catgggccat aaaaaatatt tttgcgaagg
     2641 taattcatcc ttaagtaatt ttcttttcaa aggcgggacc ggccccgccc accggctgtt
     2701 aataagttga ttcttatgcc cggattggtc ccggcggaat ttatctgaga atcccgggtt
     2761 gcatcaaaaa taattttcgt gcccggttta cagccatgta cttattttgc atcctaaaat
     2821 tctgaatcgg gagcatggag gaatttcata tcgacgaacc attccagctg gaaaacggcc
     2881 aatcgctgtc cggcatcaag atcgcgtacc atacgtacgg caacctgaat gataacgcag
     2941 ataacgtggt ctggatctgc cacgccctga cggccaattc caatgtgacc cgctggtggc
     3001 ccggtatggt cggggaaaac catatcatca atccccaaac acattttatc gtttgcgcca
     3061 atattctggg gtcctgttat ggcacgtcgg gcccgttaag cgaggatccc ggtaccggaa
     3121 agatttattt cagccatttt ccggcaatta ccatccggga tatggtgcag gcccatattc
     3181 tgctccgcaa acagctgggc atccgccgga ttgccctcct gatgggcggg agcatgggcg
     3241 ggtaccaggc cctggaatgg tgcgtgatgg aaccggaaat catccgggac ctatttctca
     3301 tcgccacttc tgccgcggaa acggcttggg ggatcgccgt gcatacggcg caacgcctgg
     3361 ctattgaggc cgatccgggc tggaaagacc tgaatgagga tgcgggatcc ggaggactga
     3421 aagcagcaag ggccatcggt atcctcacct accggaacta tatgatcatg aaagagaagc
     3481 aggcggaccc ggatcctgaa aaactggacc atttcagggc ttcctcctat atcaattacc
     3541 agggggataa gctggtggag cgtttcaatg catacagcta ctggttgctg accaaagcaa
     3601 tggataccca caatctctcc cggggaaggg gcggtgacct gaaaaaagtg ctgtcgtcca
     3661 tgcggcaacg aaccctcatc attggcatca ccagcgatat cctctgcccg cttgacgaac
     3721 aaaaatttat ttccgattat attccggatt gcaaaatgat cagcattgac tccatctacg
     3781 ggcatgacgg attcatggtg gaatcggata aaattgcgga tgggttgaag aaatggataa
     3841 accttaccag ctcagtcttc cctgataata atccatgatt ttcgacctga aaatataatt
     3901 gtaccgggcg gcggtcaggt tggtattggc ccggtcataa ttgttttttg cgatcacata
     3961 atccactgaa gtgatcaccc cgttattaaa acggatttct gccgtgcgga aagattcgcc
     4021 ataagctttt acctggtcca gataaccctg gtactgcccg taggccgaat tcatattctg
     4081 ccaggcctgc tccacctgtt gctgtaactg gttccgggag gcaccggcta aaactcttgc
     4141 gttctccagg ttgatcttgg cctgttttat attgttccgc gtggacagat aatttaatat
     4201 cgggatattc agctgcagcc cgatggactc atagcggttg tttttaaact gatcgccgaa
     4261 agagatcttc cgctgctgca cggaaaagct ctggacatca tagctggaag cgccgatatc
     4321 aacaaactga ccggtactgg tcttgaattc cgtacccggg attccggtcg tggcgatatt
     4381 cgtataattt gagttgatat tgccgtagaa cagcagcgtc gggtacagtt tgcccctggc
     4441 cacggcaacg gctttctcaa aagaccttac ccggagatcc gccgccttga tcaggggaat
     4501 ggtttgcagg gccgattgaa aaatactgtc cgaactgccc ccatactgca gtgtattggc
     4561 atccagcgga acggcctcgt aggttgcctc tttctgatag ggtatattga gcaggccgaa
     4621 gagattcact ttcgaagtct ccaggttatt gatggcgttt accaggttca cccggtcatt
     4681 ggcatactgg ccctgcagat catacaaggt agccggggca atggctccgg cgcggttttg
     4741 aatttccagc cttaccacct ggaggctgtc cactgttgcc tgctgacggg caatggtcag
     4801 ctgatcctgg ttgcttagca ccagcagata gccgagtatc acattcaggg tgatattgtc
     4861 cttctgctgt tgccaatcca ttttgccggc gtcgaagttc agcgcattct ggcggatggc
     4921 gttttgattt tgcaatccgc tgaacaaaac aagacttgca ttcaccccat agttgccgga
     4981 attgttctgc tgatcaacat aggtgttgtt caggttattt atgcttctgc cgtaattaag
     5041 tccttggctg ccgctggcat tgatttgcgg caggaggttg tctttggcct gtttgaactg
     5101 gacgccgctg gtcttcatct gaatctcact ttgtttcacc tgcaggttgt tctgaatggc
     5161 cagttcaacg cattgcttca ggttgagggt ggtagccgtg ctgtcctggc aatagcccgt
     5221 atgatgaaaa gccatggcgg ccgataatat ccagatgcga aaccacttca ttgttgtgct
     5281 catttatttg tctccgtttt ttctatccgc ccgtcaagca ggtgaacgat ccgctttccg
     5341 tagccggcat ttttttctga atgggtaacc tgaatgatgg ttaccttatc ctgctggttg
     5401 agtttggtga ataattccat gatctcttcc ccctgcttgg aattgagatt acccgtgggt
     5461 tcgtcggcca ggatcagctt gggtttggct accagggacc gggctatgcc cacgagctgt
     5521 tgttgtccgc cggataaatg ggttgggaac aaatcttttt ttcccaccat ctgaaaccgg
     5581 tcgagaatat cggccacgat ggccttgcgt tccgaggttt tgatattctg atagatcagg
     5641 ggagtttcga tgttctcgta taccgtgagc tcatcaatca ggtgataggc ctgaaaaaca
     5701 aacccgatat gggttttata gagattcgat ctttgctttt cctttaattt ataaacatcc
     5761 gtgtccataa actggtaatc gccttccgat ggcgtatcca gcattccaat gatattcaac
     5821 agggtggatt ttcccgagcc ggaaggaccc ataacggaaa taaattcccc ttccccgatc
     5881 tggagattga tatccttgag aataaaattc cggatgttac cgatattgac ccatttcgag
     5941 atatgttcta gcttgatcat aatatgtttg tcttgtttat tttctcactc cgatcttaaa
     6001 ctgttcaccg ggttcgccac cgcggcctta attgcctgga aactaacggt gatcagggca
     6061 atcaaaacgg ccagcagcgc cgcaacgaaa aatacccacc agccgatctt tatctgataa
     6121 gcaaaatctt tcagccagtt atgcattgcc caccaggcga cgggggaggc aattacaatg
     6181 gccagggcaa ccagctttat aaaatccctg gacagaagat tcacgatatt cgaaacggaa
     6241 gcgcccaaca ccttacggat gccaatttcc cgggtgcgct gcagggtgct gtaggaggcg
     6301 agccccaata agcccaggca ggatatgaag atggcaagga cggcgaaatt cacgaagagt
     6361 tttccaaatc tttcttcgct tcggtattgc ttgtcaaaaa attcatccag gaaaaaataa
     6421 ctgaaagggc gtgccggaat taaggcattc catttgcttt ctatggctgc aattgttttt
     6481 ggtaaattct gcgtggtcag gttaatggaa accaggttgc agccctgcgg ttcaatccgc
     6541 atggaaaggg gactgatttc ctgctgcaaa gaccggaagt gaaaatcttt tacgactccg
     6601 atgatctttc cttttcttcc ccactggctg aaattctggc caatggcctg ttggggagag
     6661 ctgtatccga acagcctgac tgctgcttca ttcaggatca tggcctgcgt ggtatcggtt
     6721 tgaaagtcgc gcgaaaaagg acgcccggcc accatcttta tcttatactg ggggatataa
     6781 tcaaagtcaa caaaatataa atcgaggttg gcgatctgca tttccccctt cttattctca
     6841 atttcggaat aggcgcccgg gtttccaccg cccggtacgc tggaagacat ggcgacggac
     6901 agcacattgg gcagactggc tactgcattt cggaaagact ccctggccgg atcaaaattg
     6961 gtattaatca ccagcatctg gtttttcttg aaccccagat cctggttgcg catataattt
     7021 aactgcttgt aaacaatgat cgttgcaatg atcaacccga tgttaatact gaactgggta
     7081 accaccagtc cttttcgaag aaggacaccg cgggtgccgg tggaaaagcg gcctttcaga
     7141 acagttaccg gccggaagga agacaacacc agcgccggat aaaaaccggc cagcaagcca
     7201 attccgaccg ccgccaggaa aagcagcaga ataaaacgca tgttttcaaa gatgcccgag
     7261 ctgatggttt ttcctgcgag ctgattgaaa gaatgcagga gcagggtggt ggcaccgagc
     7321 gtgaagagaa aggcaatcag gcacaacagc accgattcgc cgatgaactg ccaggtgagc
     7381 tgagtctttg ccgcacccac cactttacgg atgcccactt cctttgcgcg ttcggcagag
     7441 cgggccgtgg tcaggttaac aaaattgaaa caggcaatca gcaaaagaaa agcggcaaca
     7501 atcgaaaaga tatatacatt attgatattc cccgtattgc ttccgttccg ggttgaatac
     7561 aggtaaactt tttttaaagg ctccagaatg atcgtcggat acatgttcat ttgcttcatt
     7621 tctgatccgt tccttctttc gagaaaagca ggaagctggg cctgaagcgc cttcgcattg
     7681 gtacccggtt ttaaaagaac gtaagtggaa ttcccgtagt tcccccattg gtcatccaac
     7741 cccttgttaa agttgcgggt cagcgtgctc atggagacca gtagatctgc tttgatctgg
     7801 gaattctccg gaaagtcctt cattaccccg gtaatttttg ccggaagtcc gcctccggta
     7861 aacaatacgg tttgaccgac cggatccgca ttgccaaaat actttttcac ggccgtttcg
     7921 gataaaacca ggctgaaggg ctcattcagt gcggtgttgg ggtttccttt cagtaattta
     7981 aaatcaaaga ccttaaaaaa cagggaatct gcatacagcg agttggtctc ctggaatttg
     8041 atattgcctt ttctgaccag caattcatct cccgtagtcc gtacggcagc ttccacctgg
     8101 ggaaagtcct gcataatatg cggacccacg gcccaggacg gacccgaagt atgaattgtt
     8161 tccgaaggcg ttttgagatc ggtcaccacc cggtatatcc ggtcggcctt agtgtgaaat
     8221 gaatcatagc tcaattcaaa gttcacgtaa aggaagatca ggaaacaggc cgtcatcccg
     8281 accgttaatc ccattatatt gatgaatgag aatacccggt gccgccagag gttccggaag
     8341 gcgattttga aatagttttt tagcatttta ggccagaatg ttctctgtca ccgtctgccc
     8401 gtccagcatc cggatgatcc ggtggctata gcgggcatcg tgctcggagt gcgtgaccat
     8461 aatgatggtt gttccctgtt cattcagatc cgtcagcaat tccattactt cattcccatt
     8521 ggctgaatcc aggtttcccg taggctcatc ggccaggatc aatttgggct tattcaccac
     8581 ggcccgggca accgccaccc tctgctgctg accgccggac agctgctgcg gatagtgatt
     8641 cctccggtgc atgatctgca ttttatccag catctcttcc actctttttt ttctttcctc
     8701 gcctttaacg ccgagataga tcagcgggag ctccacgttt tcaaatacgg tgagttcgtc
     8761 gatcaggtta aaactctgaa acacgaaccc gatattcctt tttctcaggt cggctctttt
     8821 cctttcattg aagtgggcta cttctattcc gttgaacata aagcttccgt tatcgggatc
     8881 atccagcagg cccaggatat tcaataaggt tgatttaccg cagccggaag gtcccattac
     8941 ggccacgaac tccccatcgt tcacctggaa cgaaagttta ttcagcgcaa tggtttccac
     9001 ttcttctgtt ttgtaaattt tttccaggtc gtgaatcagt atcatttttt ttattttaaa
     9061 ctgttattgg ggaaattatt tatcaaaaat gagctcctgc atggttccgt aggtttcata
     9121 actgctggtc accactttgt cccccggttt gaggccctgc aatacctcat aataatcctg
     9181 gttctgattg cccagctgga tatctacccg gtaagccgat ttgccgtctt cgctcacctt
     9241 gaaaatccag ttgccgccgg tctgctggaa gaatccgccg cggggcacga gcaccgactg
     9301 cctttcgtca cttagcgcca gcaggatctg cagggtctga cctcttcgaa ggcctttggg
     9361 cacgggacct aaaaattgca tatcgacctg gaaatgcccc gcggtgatct gtgaatacac
     9421 cttgaatatc ttcagatcgt aattgctgtc ggcaaaggaa aacttgcctt tgagtccggt
     9481 gtaaatacgg gaaagataat gttcgtcaac gtccaccctc actttaaaac cgctcagcac
     9541 gtcgacctgt cccaaccgtt ctcccttgtt cttattctga cccacttcag cgtccagaga
     9601 ggtcagctgg ccgtccaccc gggcccgtac gatcaggtcg cccacttttt cgtgcattac
     9661 attcagggcc tgctgggacc ctttgaaaga ctgctggtcc gatttcagtt gctgttccgt
     9721 agaaacggag tcctgttgca tgatctgatc cgtcagtttc tttttctgca ggtaataatc
     9781 atagtcgttc tccgctttga ggtattcctg ggacccaatg gctttttgcg cgtacagggt
     9841 tttgtccaga tcgtagatcc gtttggcctc ccgcaactga ctttccacat ccgttagctg
     9901 gttcctcttg ttcaccgtat tctgctgcgc cgcattccgg gagatctgca tctgggtaag
     9961 caggttaaat acgttggtct gctgggtaac cagggcgagc tggatatcgg tattggacaa
    10021 ccggagaata ggttgtcctt tcttcacgaa tgctccgtct tccacatatt tttcctccac
    10081 ccgtccgcct tccgaggcat ccaggtaaat actcgttatg ggaagaacaa ccccgttcac
    10141 gggaatggtt tcctggaaag gacccttcgt aacggtggaa atcgtaatgc ggtcaagttc
    10201 cacattcagg cgggatttgc cggcggtgaa ataataactt ccgaaagcca gcaaaaccag
    10261 cgctacaatg cccagaatag tcagcacctt cttgagcggc catttctttt tctttataac
    10321 ccggtctacc atatttacat tattttttaa gaatcaccag gtataaagat acccggtgta
    10381 tattattaaa gctgaaacta agaaaataca ggtcatctaa atgcaaccac gtgccagaac
    10441 caaatcagaa tgatttacaa ccatttagaa aacccgatgc a
//
LOCUS       DASYCU010000037         7615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_736166, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000037 DASYCU010000000
VERSION     DASYCU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736166"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VGM24_00755"
     CDS             <1..1153
                     /locus_tag="VGM24_00755"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4153915.1"
                     /translation="SLAMNALTECGTAKEDKNSFKDKLDKVSAEISGNSGMDYATLRL
                     NCIKSDLDAVWPLYVDALTSPRFDSMEFNRIKQDAINNLKSQASQPDYAIDKMAKETA
                     FAGKDYAKDPDGTEETVSKLSASQTKAYYSSILTRSRMLIVVVGDLDRDMLEKRIQEL
                     TALIPEGKPFKQNRYSYVPAKNSFTSEKKELATNYIEGITAAPQPGTADFDAYILASR
                     IFYNRHFLEVRTNNGLSYAPIVDFNGGLSPSTSIVVSTTNPDKYKEVLDQLISKIKKE
                     GFTPAEVKNGKTIYVTSFYYKMETNAAQASSLASNEVLHNNWRRALTINEDLKKVSLA
                     DVNRAFNKYVNHITWVYQGDSSKVNPALYTGTAAKPGLPASKLKTDNKK"
     gene            complement(1157..2077)
                     /locus_tag="VGM24_00760"
     CDS             complement(1157..2077)
                     /locus_tag="VGM24_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEY4153916.1"
                     /translation="MASVIITGGSGMVGTALSKLLVSKGYSVIILSRNPSPKRAPSPA
                     IRYAAWNIEDQTINEEAVSEAEYIIHLAGAGVADKRWTAKRKKEIQESRVKGSDLIVK
                     ALKQIPNRVKAVVSASAIGWYPETATVQSVETDPPAPGFLGDTCRLWEEHIQPVTAMG
                     KRLVILRTGIVLSNNGGALPEFRKPVRMGIAAILGNGRQIISWIHLDDLCRLYLEAMT
                     DPAWSGVYNAVAPQPVTNKTMMIGLAKRIKHNFYIPLPVPGFVLRLLFGEKSIEVLKS
                     SNVSCEKIKKQKFQFIYPTLDAALRDLLRR"
     gene            complement(2179..4059)
                     /gene="ftsZ"
                     /locus_tag="VGM24_00765"
     CDS             complement(2179..4059)
                     /gene="ftsZ"
                     /locus_tag="VGM24_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY4153917.1"
                     /translation="MIQFDLPKERSSIIKVIGVGGGGSNAVNHMFSQNIEGCNFIICN
                     TDAQALAQSRVPNKIQLGPLLTQGLGAGANPDIGRQATEESLEEIKSLLEVNTKMAFI
                     TAGMGGGTGTGGAPILSKICKDLGILTVGIVTTPFSYEGRKRQQQAEEGIQLLKDYVD
                     TLLVISNDKLRHQFGNLKMKEAFSKADNVLATAAKCITDVINSTGQINVDFADVCTVM
                     RNGGVAILGSSAASGNNRAQQAIEEALNSPLLNDNDIRGAKWILININSSEGEHEFTM
                     DEVEIIQHHLISQAGPDTDVILGLGYDNKLDDQIGITIIATGFEYKDPFPRQQKTPEP
                     KEDEKIVLPLKMPSQEAGLAVEKPLVEVQEKIKTIFSINALAPELQPKLVEAKSAEQA
                     KSMGTVVVFPSGAGNREPVENPVEKNIRQEPIEWIFSQEPVPAIAEKQKDNPPQKNDP
                     FITGSGGYLSRPSNIYADEPVNHIGSLPENIPLNDQKILAPVAENPVPGEAGSPDMHL
                     VFKNEIPAADAPGAHPAQPVSGAEQPAEEPALQDEAEGLKRRAAERINKLRNLSFNMN
                     AADPNNEFETVPAYIRRNLELYNTVNNVETFYSQYEVKADQHNHGEISTINSFLDGKK
                     PD"
     gene            complement(4093..5478)
                     /gene="ftsA"
                     /locus_tag="VGM24_00770"
     CDS             complement(4093..5478)
                     /gene="ftsA"
                     /locus_tag="VGM24_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY4153918.1"
                     /translation="MNQEQPIIVGLDIGTTKIAAIAGRKNEFGKLEILGFGRANSNGV
                     KHGQVLNIDETIKAIRIALDNCYASNPNLSISDVYVGIAGHHIKSLQTRGDIVRQNTE
                     DEITQKEIDQLVADQYKTYIPAGDQIIDVIPQEYAVDNFQNIISPIGYGGVKVGANFH
                     IITGDKNAIKNISRAVEKSGLKTKDLVLQPLASAAAVMGQEDLEAGVAIVDIGGGTTD
                     LAVFYEGILKHTAVIPFGGENITNDIKTGLGVLKTQAEQMKVQFGSALSNEAKGNAFI
                     TIPGLRGMPAKEISVRNLANIIQARMSEIMDFVSYHLKQVGLDNKMLNGGVVLTGGGA
                     QLKHLIQLTEYVTGLNARIGYPTEHLASGQIEELAKPTYSTCIGLILKGYSDYEHNRK
                     QFEQSHLKMEVPRGLRSAAAEKAEMAEMETGEEEIQGTRVKNRKGIKDFWGKFKDGII
                     DLFKEEEDHAL"
     gene            complement(5512..6483)
                     /locus_tag="VGM24_00775"
     CDS             complement(5512..6483)
                     /locus_tag="VGM24_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153919.1"
                     /translation="MNLGASIKKILSVVLWCALGGAALGLLVAAINKKSSSLCKGVEI
                     QINAGGKGICLDKKDVLHLLEKEGLKDIRDKRVQSLNLHQFEMALSRHPWIKDAQLYF
                     DNNQVLNVRITERQPAARIFMVSGNSWYMDSSGKQLPLAGSIPLRLPVFTGYPAQKMG
                     LHADSALDQQIKSLSSFLNTDAFWSDAIEQVSITPSKTFELIPLIGNQVIEFGDGNDY
                     ENKFHRLLVFYKRVMTKTGFEKYSRIKVEYAGQVIGTRRGGAVSRADSLQALRNVMEI
                     IRMARRMESDTAGIREVKPLEKDQMTEQNLKSYDFPEETENSPDQKK"
     gene            complement(6484..>7615)
                     /locus_tag="VGM24_00780"
     CDS             complement(6484..>7615)
                     /locus_tag="VGM24_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY4153920.1"
                     /translation="DHPGLQYYRDRGYEVVKRSEVLGLITASSLNVCVAGTHGKTTIS
                     TLIAFILRDSGFGCTAFLGGISANYNTNFWSDERNVCVVEADEYDRSFLKLNPDIGVI
                     SSMDADHLDIYGTAAAVEEAFVEFSGRIKTGGTLISNYGLPVPHPGKNIQQYRYSLTD
                     PRADLYAENIRINEGSYWFDVAGTTWTLKNVRMNIGGRHNMENALAAIAVSKKLGLGD
                     EQILQAVDRFQGVRRRFEYHARGKGVIYIDDYAHHPKELNALIGSAKELYPERKCVVI
                     FQPHLFSRTRDFAPAFAESLDLADEVILLPVYPARELPIEGVSSKTILERMNIHKKQI
                     LDKAEVLDWVKTLTGKDKILLITAGAGDIDGLVNPIAEIING"
BASE COUNT         1728 a         2055 c         1760 g         2072 t
ORIGIN      
        1 aagcctggca atgaacgcgc tcacggaatg cggaacagcc aaagaagata aaaacagttt
       61 taaagacaag ctcgataagg tcagtgcgga gatcagcgga aattcaggga tggattacgc
      121 cacactccgg ctgaattgca ttaagagcga cctggacgcc gtatggccgc tatatgtgga
      181 tgcactcacc tctccccgct tcgacagtat ggaatttaac cgcattaaac aggatgcgat
      241 caataacctg aagtcccagg cttcccaacc ggactatgca atcgataaaa tggccaagga
      301 aacggctttt gccggaaagg actatgccaa agatccggat ggaacggaag aaacggtgag
      361 taaactgagc gcctcccaaa ccaaagccta ttattcttcg atccttaccc gttccaggat
      421 gttgattgtg gtcgtcgggg accttgaccg cgatatgctg gaaaagcgaa tacaggaact
      481 gaccgcgctt attcccgaag gaaagccgtt taagcaaaac cggtatagtt atgtcccggc
      541 aaaaaacagc tttacctcag agaaaaaaga actcgccacc aactatattg aaggcatcac
      601 cgccgctccg caacccggaa cggcagattt tgatgcgtat atcctggctt cgcgtatttt
      661 ctataaccgg cattttctgg aagtgagaac caacaacgga ttgtcgtatg cacccatcgt
      721 ggattttaat ggcggattgt ctccctccac cagcattgtg gtttccacca ccaatcccga
      781 taaatacaag gaagtgctcg atcagctgat cagtaagatc aaaaaagaag gcttcacgcc
      841 cgcagaagtt aaaaacggca agaccattta tgtcacttcc ttctattaca agatggaaac
      901 caatgcggcg caggcttctt cgctggcatc gaatgaagtt ttgcacaaca actggcggcg
      961 ggcgctgacg atcaatgaag acctgaaaaa ggtaagcctg gcggacgtaa atcgggcatt
     1021 caataaatat gtcaaccaca tcacctgggt ttaccaggga gacagcagca aggtgaaccc
     1081 ggctttatat acgggtacgg cagcaaaacc cggtcttccc gcatccaaat taaaaaccga
     1141 taataaaaag taagatttac cggcgcagaa ggtcccggag cgcggcatcc agtgttggat
     1201 aaataaactg aaatttctgt ttctttattt tttcgcagct cacgttgctg cttttcagga
     1261 cctcgatgct tttttctccg aacaggaggc ggagaacaaa accaggtacc ggcaacggaa
     1321 tataaaaatt atgcttaatt cgctttgcca gcccgatcat cattgtttta ttcgtgaccg
     1381 gctgcggcgc cacggcattg taaacgccgg accaggccgg gtcggtcatg gcctccaggt
     1441 ataaccggca tagatcatcc aggtgaatcc aggaaatgat ctggcgtcca ttgcccagga
     1501 tggcggcaat gcccatacgc acgggtttcc tgaattccgg tagggcgccg ccgttattgc
     1561 tgagtacgat cccggtccgg agaataacca atcgtttacc catcgccgtt accggttgaa
     1621 tgtgttcttc ccacagacgg caggtgtccc ccagaaaacc gggagccggc ggatcggttt
     1681 ccacgctttg aacagtggct gtttcgggat accagccgat cgccgaggcg ctgacaacgg
     1741 ctttcacccg gttggggatc tgtttgagcg ctttcacgat caggtcgctt ccttttacac
     1801 ggctttcctg aatttccttc ttccgttttg ccgtccaccg tttatcggcg accccggcgc
     1861 cggcgaggtg gatgatgtat tccgcctcgg aaaccgcttc ttcgttgatc gtctggtctt
     1921 caatattcca ggccgcatac cggatggcag gggacggggc ccgcttcggg gaaggattgc
     1981 gggaaagaat gatcacggaa tatccctttg agaccaggag ttttgaaagc gcggttccga
     2041 ccattccgga tccgccggta ataatgactg aagccatgtt attgatatta tccggttctt
     2101 ccgtcgaagt aaaaatccct ccggaaaaac cggagggaaa taaacaacta aaaacaaacg
     2161 atattggtaa agtggcggtt agtccggttt cttgccatcc aggaaactgt taatggtgct
     2221 gatctctcca tggttatgct ggtcggcctt tacttcatac tggctgtaga acgtctccac
     2281 attgttcacc gtattatata attccagatt tcggcggatg taggccggca cagtttcgaa
     2341 ctcattattc ggatccgcag cattcatgtt gaacgacagg ttgcgcaact tgttgatcct
     2401 ttctgctgca cggcgtttca ggccttctgc ttcgtcctgc aaagcaggtt cctctgcagg
     2461 ctgttccgcc ccggataccg gttgagctgg atgggcccca ggcgcatccg ccgctggaat
     2521 ttcattctta aataccaaat gcatatccgg agagccggcc tcgcccggca ccggattctc
     2581 tgccaccgga gctaagatct tttgatcgtt caaaggaata ttttcaggca gggacccgat
     2641 atgattcacc ggttcatcag cataaatatt ggatggtctg gagagatatc ctccggatcc
     2701 ggtaataaac ggatcatttt tctgcggagg gttatccttt tgtttttcag ctattgcggg
     2761 aacgggttcc tgcgaaaata tccattctat cggctcctgc cggatatttt tttccaccgg
     2821 gttttcaacc ggctcccggt tacccgcccc acttggaaag accaccaccg tacccatcga
     2881 cttcgcctgt tccgccgatt tggcttccac cagtttgggt tgaagttccg gggccagtgc
     2941 gtttatacta aaaatggtct tgatcttctc ctgtacttcc accaacggct tttcgaccgc
     3001 taatccggct tcctgggaag gcatctttaa aggaagtacg atcttttcat cttctttggg
     3061 ctccggggtc ttttgctgac ggggaaaggg atccttgtac tcaaacccgg tagcgatgat
     3121 ggtaataccg atctgatcat ccagtttgtt gtcataaccc aggccgagga tcacatcggt
     3181 atccgggccg gcctgactga tcaggtgatg ctgaatgatt tccacttcgt ccatggtgaa
     3241 ttcatgttct ccttccgagg aattgatatt gatcaggatc cattttgctc cgcggatatc
     3301 attatcattc aacaggggag aattcaatgc ttcctcaatg gcctgctgcg cgcggttatt
     3361 tccggacgcg gctgaacttc ccaggatggc tactcctccg ttacgcatca cggtgcacac
     3421 atcggcaaaa tccacattga tctgtccggt gctgttgatc acgtccgtga tgcatttggc
     3481 cgccgtggcc aacacattgt cggcctttga aaaagcttct ttcattttca ggttcccgaa
     3541 ctggtgacgc aatttgtcgt tgctgatcac cagcagggtg tccacataat ctttcagcaa
     3601 ctgaatccct tcttccgcct gctgttgcct cttccttcct tcataagaaa aaggcgtggt
     3661 aacaatgccc acggtgagaa tgcccagatc cttgcagatc ttggaaagga tcggggctcc
     3721 ccccgtgccg gttcctcccc ccatgccggc agtaataaaa gccatcttgg tattgacttc
     3781 aagaaggctt ttgatctctt ccaggctttc ttctgtggcc tggcggccga tatccggatt
     3841 tgccccggca cccagtccct gggtgagcag gggccctaac tggatcttgt tgggaacccg
     3901 gctctgggcc agcgcctggg cgtccgtatt gcagataata aaattgcagc cttcgatatt
     3961 ctgactgaac atatggttaa ccgcattgct gccgccgcca cccacgccaa tgactttgat
     4021 aatggatgac ctttcttttg gcaaatcaaa ctggatcata ataggatgat taatggttag
     4081 tatgcaaaat ttttatagcg cgtgatcttc ttcttcctta aacagatcaa tgataccgtc
     4141 tttgaatttg ccccagaaat ctttaattcc tttccggttc tttacccggg tgccctgaat
     4201 ttcttcttct cccgtttcca tttcagccat ttcggccttt tcagcggcag cgcttctcaa
     4261 cccgcgcggt acttccattt tcaggtggct ctgttcaaac tgtttccggt tgtgttcata
     4321 atcactgtac cctttcaata ttaatccgat acaggttgaa taagtgggtt tggcgagttc
     4381 ttcaatttgc cccgatgcca ggtgttcggt cggataacca atgcgggcat tgagcccggt
     4441 cacatattcg gtgagttgaa tgaggtgttt caactgggcg ccgccgccgg taagtacaac
     4501 acctccgttg agcattttat tatccagtcc cacctgtttc aaatgataag aaacgaaatc
     4561 catgatctca ctcattctgg cctggatgat gttggcgagg ttacggacac tgatctcttt
     4621 agccggcatt ccgcgaagtc caggaatggt aatgaacgcg tttcctttgg cttcattgga
     4681 aagcgccgat ccgaactgga ccttcatctg ttccgcctgc gtttttaaaa cacccaaacc
     4741 ggttttgatg tcgttggtga tattctcccc gccaaacgga attacggcag tatgcttcag
     4801 aatgccttcg taaaacaccg ccaggtcggt ggttccaccg ccaatgtcca cgatagccac
     4861 gccggcctcc agatcctcct gccccataac cgccgcagcg gaggccagcg gctgcagaac
     4921 cagatccttg gttttgagtc cgcttttttc aaccgctcgg ctgatatttt ttatcgcatt
     4981 cttatcgccg gtgatgatat ggaaattcgc tcccactttc actccgccat atccgatggg
     5041 gctgataata ttctggaaat tatccacggc gtattcctgc ggaataacat ctatgatctg
     5101 atcgcccgcc ggaatatagg ttttatactg gtccgccacc agctggtcga tctccttctg
     5161 ggtgatctcg tcttccgtat tctggcgaac gatgtctccc ctcgtctgca ggctttttat
     5221 gtgatgcccc gcaatgccga cgtacacatc cgatatactc agattgggat tggaagcata
     5281 acaattgtcc agcgcaatcc ggatggcttt gatggtttca tcgatattca atacctgtcc
     5341 gtgcttaacc ccgttggagt tagcgcggcc aaagccaagt atttccagtt tgccgaattc
     5401 atttttacgg cctgcaatgg cggcaatctt ggtggtgcca atatcgaggc ctactataat
     5461 gggttgttcc tggttcatgg tttggtttta tttaatagtt gtttttactg tttatttttt
     5521 ttgatccggg ctgttttcgg tttcctccgg aaaatcatag ctcttcaggt tctgttcggt
     5581 catctgatct ttctccagtg gcttcacttc ccggattcct gccgtatcgg actccatcct
     5641 gcgcgccatc cggataattt ccatcacgtt ccgcagggcc tgcagggaat ccgctctgga
     5701 aactgcgcct cctcttcgtg ttccgataac ctgtcccgca tattccactt taatgcggga
     5761 atatttttca aatcctgttt tcgtcatgac ccttttataa aacacgagga ggcggtggaa
     5821 tttattctca tagtcgttcc cgtctccaaa ctcgatcacc tggttgccga tcagcgggat
     5881 cagttcaaag gtcttcgatg gggtgatgct cacctgttca atcgcatccg accaaaaggc
     5941 atccgtgttg agaaaagagc tcaatgattt gatctgctga tccaatgcac tgtcggcatg
     6001 caatcccatc ttttgagccg gataccctgt aaaaacaggt aatctcagcg ggatcgatcc
     6061 ggccagcggc aactgttttc cgctactgtc catataccag ctgtttcccg aaaccataaa
     6121 aatgcgggct gccggttgcc tttcggtgat ccgtacgttc agcacctggt tattatcaaa
     6181 atacaactgg gcgtctttta tccagggatg cctgctcaat gccatttcga actgatgcag
     6241 gttaaggctc tgaacccgtt tgtcccggat atctttcaat ccctctttct ccagcaggtg
     6301 aagcacatct tttttgtcca ggcatattcc ctttcccccg gcattgatct gtatctccac
     6361 ccctttgcag agactgctgc tttttttatt gatcgccgcg accagcaggc ccagtgccgc
     6421 cccgccgagt gcgcaccaga gcaccacgga taatattttt tttatggagg ccccgagatt
     6481 catttatcca tttatgattt cagcaatcgg gtttaccagc ccgtcgatat cgccggctcc
     6541 cgcagtgatc aacaatattt tgtccttccc tgtcagcgtt tttacccagt ccagtacttc
     6601 cgctttatcc aaaatctgtt ttttatgaat gttcattctt tccaaaatcg ttttactgct
     6661 gaccccttcg atgggcagtt ctcttgccgg atacaccggt aaaaggatca cttcatcggc
     6721 caaatccagg ctctccgcaa aagcgggtgc gaaatcccta gtccttgaaa ataaatgcgg
     6781 ctggaaaatc accacgcatt ttctttccgg atacaattcc tttgcgctgc cgatcagggc
     6841 attcaactct ttcggatgat gtgcatagtc atcgatataa atgaccccct tcccccgggc
     6901 atgatattca aacctcctcc gcactccctg gaagcggtcc accgcctgca ggatctgctc
     6961 atcccccaat cccagtttct ttgaaaccgc gatggccgcc agtgcgttct ccatattatg
     7021 cctgcccccg atattcattc tgacattttt cagcgtccag gttgtacccg ctacgtcaaa
     7081 ccaataactg ccctcgttta tccggatatt ttctgcatag aggtcagccc gcggatccgt
     7141 caggctatac cggtactgct gaatattttt tcccggatgc ggaaccggca agccgtaatt
     7201 gctgatcagg gtcccgccgg tttttattct gccactgaac tcgacaaatg cttcttccac
     7261 cgcagcggcc gttccgtaga tatccaggtg atccgcatcc atggagctga tcaccccgat
     7321 atcgggattc agcttcagaa aactccggtc atattcgtct gcctccacca cgcatacatt
     7381 tctttcatca ctccagaaat tcgtgttgta gttagcggat attccgccca gaaaagcagt
     7441 acagccgaag ccgctgtccc gcaggatgaa cgcaatcagg gtgctgatcg tggtcttccc
     7501 gtgtgttccc gccacacaaa cattcaaaga acttgcggtg atcagtccca aaacctcgct
     7561 tcgtttcacc acttcatatc cccggtcgcg gtaatattgc aaacccggat gatcc
//
LOCUS       DASYCU010000038         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_591371, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000038 DASYCU010000000
VERSION     DASYCU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591371"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1277
                     /locus_tag="VGM24_00785"
     CDS             186..1277
                     /locus_tag="VGM24_00785"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY4153921.1"
                     /translation="MQYKVIGLMSGSSLDGLDIVYVHFEETAGKWDFEIRETACIPYS
                     SEWQAKLSGATGLAAREYLLLHTEYGHYLGAAVNAFIEERGLAYQVQLVASHGHTSFH
                     IPEKKVTAQLGEGAAIAAVTGIHSITDFRSSDMALGGQGAPVVPIGEKLLFAEYDLFL
                     NIGGIANLSISRPEPAGFDVCPANRILNLLAAGTEKGYDEEGKLASAGSLQEALLGQL
                     NGFAYYQRPFPKSLSNEFGLKEIFPVIKKANLSKEDALRTYVEHIALQISDAVSRLLR
                     HAGREGEGLKLLVTGGGAHNRFLLERLKAALPVKRIELSVPAKELVDYKEALIIALMG
                     ALRWREEENVLSSVTGSTRNNINGAIWSS"
     gene            complement(1274..>1996)
                     /locus_tag="VGM24_00790"
     CDS             complement(1274..>1996)
                     /locus_tag="VGM24_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEY4153922.1"
                     /translation="DDEPLARSIIREYLLQYPQIQLLTECNDGFEGVKAIQQYQPDLI
                     FLDIQMPKINGFEMLELIEDPPAVIFTTAFEEYAIKAFESHAIDYLLKPFSRERFNKA
                     INKLFAQEKSGTVKTSLTSFLDSAARSPAEQNRVVVRLSGKIRIIPVDDIHYLEAADD
                     YVKIHTKEGSFLKNKTMGHFETVLKNDQFVRTHRSYMVNISEITRIDPYEKENHLAIL
                     RSGARVPVSKSGYLKLKSVLGL"
BASE COUNT          496 a          456 c          541 g          503 t
ORIGIN      
        1 gatttcgccg gaaccgcatt ctgtcctgtt gcagggtttc atcgcgtaac tctgcaaccc
       61 aatcgcagag gatggaatag tcccgactca gatttatcac catatataaa aattaaaaat
      121 ccgatattcg aaaagaggcc ggtgataaaa ctaaatcaaa agatcgaaaa agaaaatcat
      181 ttcctatgca gtataaggtg attggattga tgagcggaag ctcgctcgac ggattggata
      241 ttgtttatgt gcatttcgaa gagaccgcgg gaaaatggga ttttgaaatc cgggaaacgg
      301 cctgtatccc gtattcctcc gaatggcagg caaagctctc gggggcgacc ggccttgccg
      361 ccagggagta cctgttgctg catacggaat acgggcatta cctgggcgcg gccgtaaatg
      421 ctttcataga agaaagggga ttggcttacc aggttcagct tgttgccagc catggtcata
      481 ccagttttca tattcctgaa aaaaaggtga ccgcccagct gggcgagggc gcggccattg
      541 cggccgtcac gggcattcat tccataacgg atttcagaag cagcgatatg gcattgggcg
      601 gccagggtgc gccggtggtt cccatcgggg aaaaattgtt gtttgcagaa tatgatcttt
      661 ttctgaatat cggcggaata gcgaatctct ccatcagccg gcccgaaccg gcgggctttg
      721 atgtatgccc ggcgaaccgg atcctgaatt tactggctgc cggtacagaa aagggatatg
      781 atgaagaggg gaagctggct tccgcaggat cccttcagga ggcactgctt ggtcaactca
      841 acgggttcgc ctattatcag cggccctttc ccaaatccct ttcgaatgag ttcggcctga
      901 aagagatctt tccggtgata aaaaaagcaa atctttcgaa ggaagacgca ttgcgcacgt
      961 atgttgagca tatcgcgctg cagatctcgg atgcggtaag ccggttatta aggcatgccg
     1021 gcagggaagg ggaagggctg aaattgctgg tcaccggcgg aggagcgcat aaccggttcc
     1081 tgctggaacg gttaaaagcg gcattgccgg taaagcggat tgaactttct gttccggcga
     1141 aggaactggt ggactataag gaagccctga tcattgccct gatgggtgcg ctccgctggc
     1201 gggaggaaga gaatgtcctt tcctccgtta cgggttcaac ccgaaacaat atcaatgggg
     1261 caatctggag cagttaaagg ccgagaacgg atttcagttt cagatatcct gatttgctga
     1321 ccggaacccg cgcaccggac ctcaatatgg ccaggtgatt ttctttttcg taagggtcga
     1381 tgcgggtgat ctcggaaata tttaccatgt aggagcggtg ggttcttaca aactggtcat
     1441 ttttcagcac ggtttcaaaa tggcccatgg tcttattttt cagaaaagat ccttccttcg
     1501 tatgaatttt tacatagtca tccgccgctt ccaggtaatg gatatcatct accggaatga
     1561 tccggatttt tccggaaagc cggaccacca cccggttctg ttcagccggg gaccgagcag
     1621 ccgaatccag aaaagaggta agactggttt ttacggttcc gcttttttcc tgcgcaaaca
     1681 atttattgat ggccttgtta aaccgctccc ggctgaatgg ttttaacaga taatcgatcg
     1741 catgactttc gaaagccttg atggcatatt cttcaaaggc cgttgtgaag atcaccgcag
     1801 gcgggtcttc aatcaattcc agcatttcga agccattgat cttcggcatc tggatatcga
     1861 gaaagatcag gtcgggctgg tattgctgaa ttgcctttac tccttcaaat ccatcattgc
     1921 attccgtgag taactggatt tgcgggtatt gcaggaggta ttcccggata atggaacgcg
     1981 cgagcggttc gtcatc
//
LOCUS       DASYCU010000039         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3924801, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000039 DASYCU010000000
VERSION     DASYCU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3924801"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1291)
                     /locus_tag="VGM24_00795"
     CDS             complement(8..1291)
                     /locus_tag="VGM24_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4153923.1"
                     /translation="MLNRKTAPAITDAVKFHLDLPAHEKVALRNGVDVFLVNMGTLDT
                     LMMNIVFYAGNCFESDNLVASATNFLLKNGTQAKNAYEINEYFEYYGAYLSRQAGHET
                     AELTLHCLNKHVDELLPVVAELIHDSVFPAEELKIFKKNMQQRLEVNLRKSDFVAGRL
                     IDACLFGKSHPYGRYSSIEEYDALNVEQVRGFYKKYYRQGRCMIFVAGLLPDNIVEQI
                     EKHFGSLPFAGSARPDLPEVPMKPAAQKKYHIVNDPDGVQGAIRIARNFPNRHHPDFQ
                     KMQVLNNLFGGFFGSRLMANIREDKGYTYGIHSYLTNLIQESCLMISTEAGKEVSEAT
                     IKEVYLEMDRLRNEPIPEEELQTTRNFMIGTLLGDLDGPFQVAGRWKSLMLNGLKEDY
                     FYRGVELIKTITPADLQELANKYLRPDDFYELVVL"
     gene            complement(1312..2553)
                     /locus_tag="VGM24_00800"
     CDS             complement(1312..2553)
                     /locus_tag="VGM24_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4153924.1"
                     /translation="MPIPFKKFTLSNGLRFVVHEDRSTPMAVLNVLYDVGARDEDPGQ
                     TGFAHLFEHLMFGGSVNIKDFEAPLQMAGGENNAFTTNDLTNYFIQIPAENVETAFWL
                     ESDRMLSLAFSEKSLDVQRKVVSEEFKEHYINRPYGDVWFKLRELTYQVHPYRWMTIG
                     KELSHIENARLNDVKRFFFKHYNPANAILVLAGNVRTDQALALAEKWFGSIPAGEKYN
                     RRLPEEPVQLSPRTMEVKTDVPLDALYKAYHVGSRTDPAYYAMDLITELLGGGGSARL
                     FQSLVKEKKLFSNIECYHLGSSDPGLLVIEGKLIRGVRMEEANGAVEEELRKLNAAKI
                     EEKELTKVKNKTESAILFEDMSLMNRASNLAMYELMGDAEYMNTELQKYMDVTGEEIL
                     RTGKLIFDEKNSNSLFYYSNN"
     gene            complement(2625..>3285)
                     /locus_tag="VGM24_00805"
     CDS             complement(2625..>3285)
                     /locus_tag="VGM24_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEY4153925.1"
                     /translation="SIVNIPVESARIRDDKERFCRFYFDTGAGLCLLLSSDFTKDSTI
                     FSSDKKMLLTQAQGMGGKANMMITTMKEFRFGPYRFRNVPTHIFDDVYNITSYPYLAG
                     LIGNDVLRRFNIILNYDKRTFYLTPNTHFRDPFDYSYTGLGLYWTDGQIRVGDVMKDS
                     PADLAGFRVDDVVIGMNNNLSQNLQLYKSMMQNVGDKIKFLISRPEGLLEINLKVKSI
                     M"
BASE COUNT          792 a          812 c          786 g          895 t
ORIGIN      
        1 taacccctca caacacaacg agttcataaa aatcatccgg cctcaggtat ttattggcca
       61 gttcctgaag atctgccggc gtgatcgttt tgatcagttc gactccccgg tagaaatagt
      121 cttctttaag tccgttcagc atcaggcttt tccatctgcc cgccacctgg aaagggccgt
      181 ccagatcacc cagtaaagtg ccgatcataa aattccgggt ggtttgcaat tcctcctccg
      241 ggatgggttc attgcgaagc cggtccattt cgaggtagac ttctttgatg gttgcttctg
      301 aaacttcttt tcctgcctcg gtggaaatca tcagacagct ttcctggatc aggttcgtca
      361 gataactgtg aatgccatag gtatatccct tgtcttcccg gatattggcc attaagcggg
      421 aaccgaaaaa tccgccgaac aggttattca ggacctgcat tttctggaag tccgggtgat
      481 gccggtttgg aaaattccgg gcgatccgga tggcgccctg aacaccatcg gggtcattca
      541 ctatatgata ctttttttgg gcagccggct tcatggggac ttccggaagg tccggtctgg
      601 ccgatccggc aaagggcagg cttccaaaat gtttttcgat ctgttcaacg atattatccg
      661 ggagcaggcc ggcaacaaag atcatacatc gtccctgccg gtaatatttt ttataaaatc
      721 ccctcacctg ttccacattc agcgcatcat attcctctat ggaactgtac cggccgtaag
      781 gatgcgattt accgaaaaga caggcatcta tgaggcgtcc ggcaacgaag tcgctttttc
      841 tcaggttgac ttccagccgc tgttgcatat tttttttgaa gatcttcagt tcttctgccg
      901 ggaaaacgga atcatggatc agttcagcca ccaccggcag caattcgtct acatgtttat
      961 tcaggcagtg cagggtcagt tccgccgttt cgtggcccgc ctggcggctc agataggccc
     1021 cgtaatattc aaagtattca ttgatttcgt atgcgttttt agcctgagtg ccgtttttca
     1081 gcaaaaaatt ggttgcactg gccaccaggt tatcggattc aaaacagttt cctgcataaa
     1141 aaacgatatt catcatcagt gtatccaggg ttcccatatt gaccaggaaa acatctaccc
     1201 cgttgcgaag cgccactttt tcatgcgcgg gaagatcaag atggaatttt accgcgtcag
     1261 taatcgccgg cgcagtcttt ctgttcaaca tgctgtattc agttaaaaaa gttaattgtt
     1321 gctgtagtaa aacaggctgt tgctgttttt ctcgtcaaaa atgagcttgc cggtgcgaag
     1381 gatttcctct ccggtcacgt ccatgtattt ctggagttcg gtgttcatat attctgcatc
     1441 tcccatcagc tcatacatcg cgaggtttga cgcccggttc atcagactca tatcttcgaa
     1501 aagaatcgcg gattccgttt tatttttcac cttggtcaat tccttctctt cgattttcgc
     1561 cgcgtttaat ttccggagct cttcctccac tgccccattg gcttcttcca tccggacacc
     1621 ccggatgagc ttgccttcga taaccagcag tccgggatca gaagacccga ggtgataaca
     1681 ttctatattg ctgaaaagct ttttttcctt gaccagggat tgaaaaaggc gggctgagcc
     1741 gccacctccc agtagctccg tgatgaggtc catggcgtaa taggccggat cggtccgcga
     1801 gccgacatga tacgctttat ataaggcatc cagggggacg tccgttttta cctccatcgt
     1861 gcggggggaa agctggacgg gttcctcggg caggcggcgg ttgtattttt cacctgccgg
     1921 aatgctcccg aaccattttt cagcaagggc cagcgcctga tcggttctta cattgccggc
     1981 aagcaccagg atggcattgg ccgggttata atgtttgaag aagaaacgct ttacatcatt
     2041 cagcctggcg ttttcaatat gcgaaagttc cttcccaatg gtcatccagc gatagggatg
     2101 cacctggtag gtaagctccc ttaatttaaa ccacacgtcg ccgtaaggcc ggttgatata
     2161 gtgttcctta aattcttccg aaacgacctt acgttgcaca tccaggcttt tttcactaaa
     2221 agccagggac agcatccggt cgctttccag ccagaaggcc gtttcaacat tttcagcagg
     2281 aatctggata aagtaattgg tcaggtcatt ggtggtaaag gcattgttct ccccgccggc
     2341 catctgaaga ggagcttcaa aatccttaat attcaccgaa ccgccgaaca tcaggtgttc
     2401 gaacagatgg gcaaaaccgg tctggccggg atcttcatcg cgagcaccga cgtcgtacaa
     2461 aacattcagc acagccatcg gtgtggaacg gtcttcgtgt acgacgaagc gcaatccatt
     2521 acttaaagtg aattttttaa aaggtatggg catgaaatac gctttcgttt ccgttgaaaa
     2581 gaaagtgcaa gttaataaga cttcaagaac cggaaaggaa agatttacat gatgcttttg
     2641 actttcagat ttatctccag caggccttcg ggccggctga tgaggaattt aattttatcc
     2701 cccacatttt gcatcatgga tttataaagc tggagattct gcgacaggtt attattcatg
     2761 ccaatgacga catcgtccac ccggaagccc gccaggtcgg ccggggaatc cttcatcacg
     2821 tcccctaccc ggatctgccc gtcggtccag tataaaccca atccggtata agaataatca
     2881 aagggatcgc ggaaatgggt attcggagtc aggtaaaagg tccttttatc gtagttcagt
     2941 ataatattaa atctgcgcag cacatcgttc ccgatcaatc ccgcgaggta aggataggag
     3001 gtaatattat aaacgtcgtc aaaaatatgg gtgggtacat tccggaaccg gtaaggtccg
     3061 aaccggaatt ctttcatggt ggtgatcatc atattggcct tgccgcccat gccctgggct
     3121 tgggtaagca gcattttttt atcgctgctg aaaatcgtgc tgtctttggt gaagtcggat
     3181 gaaagcagaa ggcagaggcc ggctccggta tcgaaataaa agcggcagaa ccgttctttg
     3241 tcatcccgga tccgggcaga ttccaccggg atgttcacta tggaa
//
LOCUS       DASYCU010000040         4325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4794843, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000040 DASYCU010000000
VERSION     DASYCU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4794843"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /gene="ssb"
                     /locus_tag="VGM24_00810"
     CDS             complement(<1..425)
                     /gene="ssb"
                     /locus_tag="VGM24_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY4153926.1"
                     /translation="MIKLQVIGNLGKDCVTNLVNGKNVINFNVAHTEKFRDAQGNQKE
                     KTVWVDCAYWTDRTAIAPYLKKGTQVYAEGTPDLRTYQKTDGTQGVSLTLRVQSVQLL
                     GSRNTNDSAGYGNDIQNQASPSMSGIPSPGELSAPVDDLP"
     gene            530..1636
                     /gene="mnmA"
                     /locus_tag="VGM24_00815"
     CDS             530..1636
                     /gene="mnmA"
                     /locus_tag="VGM24_00815"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY4153927.1"
                     /translation="MSRNGKVLVAMSGGIDSTITALMLHDQGYEVIGITMKTWDYASS
                     GSSKKETGCCNLDSFNDARMAAVQHGFPHFVLDIREEFGTAVIDNFVEEYLAGRTPNP
                     CVMCNTHIKWRALLKRADALDCDFIATGHYANIRQQGGGRYFLSRGIDELKDQSYVLW
                     GLQQDLLSRTLLPLGNFRKTQIRQMALDYGYPELAKKSESYEICFVPDNDYRGFLKKK
                     MPALEQSVEGGSFVDVHGKIIGKHRGYPFYTIGQRKGLDIALGRPVYVTAIHPENNTV
                     VLGEETDLAGQTLLVGGVNLMKYDAVPDAMEALTKIRYKDKGSLSNLYPGDKQIRVQF
                     YEPVKSIAPGQSAVFYEGDDVIGGGIIRRSVNGL"
     gene            1664..2140
                     /locus_tag="VGM24_00820"
     CDS             1664..2140
                     /locus_tag="VGM24_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY4153928.1"
                     /translation="MAKAHQYNTHLRWTGNTGKGTENYRAYERAYTVSIQGKPEMTGS
                     SDPAFRGDPARYNPEEMLVVALSACHMLSFLHFCSAAGVVVVDYSDAATGTMEESADG
                     NGRFTEVLLKPMLIVKDASMIEKANALHEKAHQFCFIANSCNFPVLHQPVCTAENN"
     gene            complement(2160..2663)
                     /locus_tag="VGM24_00825"
     CDS             complement(2160..2663)
                     /locus_tag="VGM24_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153929.1"
                     /translation="MSVQFLGEKMKELENALFVSEARSLSKLPTHIVQIAEVDEEGNA
                     WFIIPRPTQIMESFDREMPAKLDFFKKGKGFYLKLRGLASIVHSEELLPRTRVFQELS
                     GSFKKKELVAVKLKMQSAEYFENTPKSPSNPVVNIGSMLYNWLLNPLFNSRQPQWVTI
                     PVYAENN"
     gene            complement(2967..4298)
                     /locus_tag="VGM24_00830"
     CDS             complement(2967..4298)
                     /locus_tag="VGM24_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4153930.1"
                     /translation="MKKFYLLILTTVFLFTSLLSRAQGTYTSISPNSGPGAPYTLDDP
                     RFWTGNVAPPNPCNLCTIVINSSVNMVPNDGSTTSLAGGGVSLNDIVISNSTLQVNGG
                     VNLNVETYVEMVNTNVLIGSDATTAASVFINDQVVLSGTSTIQLANVNTFVNANNDFS
                     HPINGPLKRYNGASGVAGVYYLDASDPKGYDFVLSLPGIGNSTNMNIDGASPTNLSMY
                     TINCDGNGPPNSNTCLAGIINGPSLSFYDAVNKYLSFQQTTTLPVVLVQFLATRNADG
                     SIKLSWATAEEINSASFEVERSADQGTWEKIGTVAAKGYSSTTANYSYIDQSPFPGNN
                     YYRLKMIDLDGRFKYSKVVAVTSDKDNRPLVIYSNPFNDQIRMKVNVDRAQNLILTVS
                     DIIGKTYLKQSYNAQAGGNLINLVPAGAAGGMYILHIQGNSYEQTVKLVRQ"
BASE COUNT         1099 a         1044 c         1086 g         1096 t
ORIGIN      
        1 ggaagatcgt cgaccggggc agagagctcg cccggagaag gaatgcctga catacttggg
       61 gaagcctggt tctggatatc atttccatag ccagcactat cattcgtatt ccggctgccg
      121 agcaattgta cactctgtac acgcagcgtt aaggatacgc cctgcgttcc atctgttttc
      181 tggtaggtgc gcaaatccgg agtgccttct gcatacacct gtgttccttt tttcaggtaa
      241 ggagcgatcg ctgtccgatc cgtccagtaa gcacagtcca cccacacggt tttctccttc
      301 tgatttccct gggcatcccg gaatttctcc gtatgcgcca cattgaaatt gatcacattt
      361 ttaccattca cgagattggt cacacaatct tttccaagat ttccgatgac ttgcaatttg
      421 atcataactt aaaatttagc tgcgtactta aaattgtttg aaatggcaat ttacaggttt
      481 tagaaggtca ataaaatact attttgcggg aataagtatc tttgcgccca tgagcagaaa
      541 tggaaaagtg ctggtagcca tgagcggggg tatcgacagt accatcaccg ccctgatgct
      601 tcatgaccag gggtatgaag tgatcggcat tacgatgaag acctgggatt acgcctcgtc
      661 cggatcttcc aaaaaagaga ccggctgctg caacctggat agcttcaacg atgcccgtat
      721 ggctgccgtg cagcacgggt ttccgcattt cgtgctcgat atccgggagg aattcggaac
      781 cgcggtgatc gataattttg tggaagaata cctcgccgga cggaccccga atccctgcgt
      841 gatgtgcaat acccatatta aatggcgcgc cctgctgaaa agggccgatg cgctggactg
      901 cgattttatc gccaccgggc attatgcaaa tattcgtcag cagggcggcg gacggtattt
      961 cctcagcagg ggtattgatg aattgaaaga tcagagttat gtgctttggg gcctgcagca
     1021 ggatctgctc agccgcaccc tgcttcccct gggtaatttc cgcaagaccc agatccgtca
     1081 gatggccctg gattatggct atcccgaact ggccaaaaaa agcgaaagct atgagatctg
     1141 ctttgtcccg gacaatgact accggggttt cctgaaaaag aaaatgcccg cactcgaaca
     1201 gtcggtggaa ggaggaagtt ttgtggatgt tcatggaaag atcatcggca aacaccgcgg
     1261 ctatccgttt tataccatcg gccagcggaa aggactcgat attgccctgg gcagacccgt
     1321 gtatgttacg gccattcatc cggaaaacaa caccgtggtt ttgggagaag aaacggatct
     1381 ggccggacaa accctcctgg tcggcggcgt gaacctgatg aaatacgatg ctgttcccga
     1441 tgcgatggag gccttgacca aaatccggta caaggacaaa ggaagcctga gtaatttata
     1501 tcccggagac aaacagatcc gcgtacagtt ttacgagccg gttaaaagca tcgcccccgg
     1561 tcaaagcgcc gttttttacg aaggagacga tgtgatcgga gggggaataa tccggcgatc
     1621 ggtcaatggc ctgtgaatac cctccggaat taaatgctca actatggcta aagcccatca
     1681 atacaatacc catctccgct ggaccggcaa taccggaaag ggaacggaga attaccgggc
     1741 ttatgaaaga gcctacaccg tttcaataca aggcaaaccg gagatgacgg gttcttcgga
     1801 tccggccttt cgcggcgacc cggccaggta caacccggag gaaatgctgg tcgttgcctt
     1861 gtctgcctgc catatgcttt cttttctgca tttctgctcg gcagccggcg tagtggtggt
     1921 ggattattcg gatgccgcca cggggaccat ggaagaatca gccgacggaa acgggcggtt
     1981 tacggaagtg cttctgaagc cgatgctgat cgtgaaagac gcttccatga tcgaaaaagc
     2041 aaatgcgctt cacgaaaaag cgcatcagtt ttgttttatt gccaactcct gtaattttcc
     2101 ggtgctgcat cagccggttt gtacggccga aaacaattag cggaaggaaa tccagccctt
     2161 taattattct ccgcatagac cggaatcgtt acccattgcg gctgcctgct gttgaataaa
     2221 ggattcaaca gccagttgta cagcatactc cctatgttaa ctaccgggtt tgaaggagat
     2281 ttgggtgtat tttcaaaata ttcggccgat tgcattttga gtttcacggc caccagttcc
     2341 ttctttttaa aggatccgga caattcctgg aagacccggg tgcggggcaa taattcttcc
     2401 gagtgaacga tcgaagccag accgcgcagc ttcagataaa atcccttccc tttcttaaaa
     2461 aaatccagct tggcgggcat ttccctatca aaggattcca taatctgcgt gggccttgga
     2521 ataatgaacc aggcatttcc ttcttcatcc acttctgcaa tctgcacgat atgcgtggga
     2581 agttttgaca aagacctggc ttcagaaaca aatagagcat tttccaattc cttcatcttt
     2641 tctccgagaa actgaactga catatcctgt accatagttg cttagtttcc cccatagagg
     2701 caagccctgt accaaaccgg gaaattatga taatgaggga tttgcaaaat ttcgcccctg
     2761 ctttttgttc ctgttttggg agtttatttc cagaaaagga cgatccgtcc ctttaattcc
     2821 caacccggaa cgagggcccc attatcggtt atgcataacc gttagtaatc ccgagggcag
     2881 atcttttaaa aggcaatgcc actgtcaggg gatgctcaat cctcccgaac agtggcatac
     2941 aactgaatcg tgattatcaa atcatattac tgcctgacca gcttgaccgt ctgttcatag
     3001 gaattaccct gaatgtgcag aatatacatt ccgccggcgg cacccgctgg taccaggttg
     3061 atgaggtttc cacctgcctg ggcgttatat gattgtttta aataggtttt accaataatg
     3121 tccgaaacgg tcagtattaa attctgcgcc cggtccacat tcactttcat gcggatctga
     3181 tcgttgaagg gattgctgta gatgaccagg ggccggttgt ctttatcgga agtaacggca
     3241 accactttac tgtatttgaa tcttccgtcc aggtcaatca tcttcaggcg gtagtaatta
     3301 ttgcccggga acggagactg atcaatataa ctatagtttg ccgtagtgga ggaataccct
     3361 ttggcggcca cggtgccgat cttttcccag gttccctgat ccgcgcttct ttccacctca
     3421 aaggaggcgg aattgatttc ctcggcggtg gcccaggaaa gcttgatgct gccatccgca
     3481 ttccgggtgg ccaggaattg aaccaggacg accggcaaag tggtcgtttg ctggaaagat
     3541 aaatatttat taactgcatc atagaaggag agagaggggc cattaatgat gcctgccaga
     3601 caggtatttg aattgggcgg gccgttcccg tcgcagttaa tcgtatacat ggacaaattg
     3661 gtcggggacg caccatcgat attcatattg gtggaattgc caataccggg cagacttaaa
     3721 acaaaatcat atcccttggg atccgaggca tccagatagt aaacaccggc aacgccactg
     3781 gcgccgttgt atcttttcag cggtccgttg atcggatggc tgaaatcatt gttggcattg
     3841 acaaaggtat tgacattggc cagctggatg gtggaagtgc cgctgagaac cacctgatcg
     3901 tttataaaaa ccgaggccgc ggtggtcgca tcacttccga taaggacgtt cgtattaacc
     3961 atttccacat aggtttccac gttcaggttc actcccccgt ttacctgtag ggtactgttg
     4021 gaaattacta tatcatttaa agacactccg ccgcctgcca gcgatgtcgt ggaaccgtcg
     4081 tttggcacca tgttgacgct ggaattaata acaatggtgc agagattgca gggattgggg
     4141 ggggccacat taccggtcca gaaccgcgga tcatccagcg tgtagggagc acccggccct
     4201 gaattcggag aaattgaagt gtatgtaccc tgcgcccgcg acaaaagaga agtgaataga
     4261 aaaaccgtgg tgagaatcag aaggtaaaat tttttcataa tcaattcatt aagctgttaa
     4321 tcggt
//
LOCUS       DASYCU010000041         4562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3635744, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000041 DASYCU010000000
VERSION     DASYCU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635744"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VGM24_00835"
     CDS             <1..1676
                     /locus_tag="VGM24_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153931.1"
                     /translation="SAVSAQSVGLGIAEPDASAMLDMTSTTKGLLVPRMNSSNIHSIP
                     VPAKGLLVYDSVKNELMVNMGTSATPDWQPIAANNVWGLTGNSHTDSAVNFLGTTDDQ
                     PLHFRINDQWAGMIDSASNSTYMGYGSGRNGAGTLGNTAYGFKALEQNAAGNYNTALG
                     VQTLSVNANGTDIGSYNTAVGYQALMNNPGTIANTAVGYQALFNNSTGFNTAIGYQAL
                     YNGGGGSNTATGAQALFNGGGNSNTAIGSKALYNNAASFNTASGYEALFTNVSGSSNT
                     AAGYQAMRSNYSGQYNAAFGSGALYANSYGSYNTAIGFNTLANTTGSDGNTALGYNAG
                     ATYDNGYYNCFIGSETDANGAGYYNCIALGHGTIVTAPSMMSVGNGATGSIGGQVGWS
                     TISDGRVKRNIQENIPGLDFIMKLRPISYTIDLAAVDAIVQPPGQKSGTGLLGEGSAQ
                     PMATAKPFSPQMIAARKAKEQIVYTGFAAQEVEQAAQSLHYNFSGVDAARNDKDLYSL
                     RYGAFVVPLTKAVQELSARNDRLEKENELLTKQSQQIRKRIAILKQKMN"
     gene            1700..3061
                     /locus_tag="VGM24_00840"
     CDS             1700..3061
                     /locus_tag="VGM24_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4153932.1"
                     /translation="MKKVLIATAAFVLMLFSTKNARAQFTIAGGAQFSMSGNLQLTLS
                     NTDLVNNGTLSAGTGTISFTGNAPSGILGSQPTQFYELKISKDAGNSVTLQRPIGVGR
                     QITFISGFLDLNGYDADLGNTGLLNGEQESSRVTGANGGELIFNGPLNAPAAVNPGNL
                     GILISSSQNLGNTTIKRGHQSQTSSGGTGSSILRYYDIVPANNSGPNATLRFQYFEGE
                     LNGLDENALILWEKTAAQPWTALGFDSRNINPHYVEKTGVSSFDRFTLSAPGDPLPVI
                     FNFFRARCNGNQVELTWQTAQEENSHYFTAERTADGNTWIPLGNIPAAGNSPNEHDYS
                     FTDQAPIQKGQYRIAEYDMDGRVQYTKIAAPGCSILDTFRVWPNPVRGTLYINITADT
                     VSAAIIKLFDSKGATIKRQTGILFPGNNLLTLDLRAVAQGVYFLSIIRNNGQVQVREL
                     IKE"
     gene            complement(3078..3635)
                     /locus_tag="VGM24_00845"
     CDS             complement(3078..3635)
                     /locus_tag="VGM24_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY4153933.1"
                     /translation="MGKLKLQMQISLDCVTTDRGGANFSWDSEVKSFVLANIDGVDRI
                     ILGRNTANGFVPHWASISAKPDDPDYEIGKRLTEIPKIVFSKTLAKSSWPNTELARGE
                     TVEEVQRLKKRAGGDLIVYGGSSFVSSLIKEGLIDQYYFLLNPVALINGETIFKSLKG
                     KLVLTLLESRTFACGTVLLVYEPKS"
     gene            complement(3741..4097)
                     /locus_tag="VGM24_00850"
     CDS             complement(3741..4097)
                     /locus_tag="VGM24_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEY4153934.1"
                     /translation="MKNIPFQTIDWTSIEKVEYKGETGSAFWQTIQLDGLRVRLVEYS
                     SGYLADDWCRKGHIVHCLEGEFISEQRTGERMKFSRNETCIVSDERSEHRSISQNGVK
                     LLIIDGDFLKYEPADK"
     gene            4190..4375
                     /locus_tag="VGM24_00855"
     CDS             4190..4375
                     /locus_tag="VGM24_00855"
                     /inference="COORDINATES: protein motif:HMM:NF018826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HEY4153935.1"
                     /translation="MFTIEQIKTAHANVKTGADYPRYVQEIIALGVTGYETWVGDGNT
                     IYFGAGNILVVESIAKP"
BASE COUNT         1186 a         1238 c         1092 g         1046 t
ORIGIN      
        1 actccgctgt gtccgcacaa agcgttggcc tcggcatcgc agaaccggat gcctcggcca
       61 tgctggacat gaccagcaca accaaaggtt tgctggtacc caggatgaat tcttccaaca
      121 tccactccat tcccgttccc gcaaaaggat tgctggtcta tgattcggta aagaacgagc
      181 tgatggtgaa tatgggcacg tctgccacgc ccgactggca gccgatcgcg gcgaacaatg
      241 tctggggact gaccggaaac agccacaccg acagcgccgt caattttctt ggcacaaccg
      301 acgatcagcc gctgcatttc cggataaatg atcagtgggc aggtatgatc gattcggcga
      361 gcaacagcac gtatatgggc tatggatcgg gcaggaacgg tgccggaact ctcggcaaca
      421 ccgcctacgg ctttaaggcg ctggaacaga atgccgctgg aaattacaat acggcactgg
      481 gggtccagac cctttccgtt aacgccaatg gaaccgatat aggcagttac aatacggccg
      541 tcggctacca ggccctcatg aataatcccg gaacgatcgc gaacaccgct gtcggctatc
      601 aggcactttt taacaacagc accggattca acacggccat cggttatcag gctttataca
      661 atggcggagg cggttccaat accgccaccg gtgcgcaggc ccttttcaat gggggtggca
      721 acagcaatac cgccatcgga tcaaaagccc tctataataa cgccgcgagc tttaacaccg
      781 ccagcggtta cgaagcgctt ttcacgaacg tttccggaag ctccaacacc gccgccggat
      841 atcaggccat gcggagcaac tactcaggcc aatacaacgc cgcattcgga agcggagcgc
      901 tttatgcaaa ctcgtatgga agttataata ccgccattgg attcaatacc ctggcaaaca
      961 ccaccggctc cgacggcaac accgcgttag gatataatgc gggggcaacc tacgacaacg
     1021 gctactacaa ttgctttatc ggttcggaaa ccgacgcgaa cggagccggt tactacaact
     1081 gcattgccct gggtcacggc accatcgtca cagcgcccag catgatgagt gtgggaaatg
     1141 gcgcaacggg ttccattggc ggccaagtgg gctggtccac catctcggac ggcagggtga
     1201 agagaaatat tcaggagaat attcccggcc tcgatttcat catgaaacta agacccatca
     1261 gctatacgat cgacctggcc gcggtggatg ctattgtaca gccgcccggt caaaaaagcg
     1321 gaaccggcct tttaggggag ggctctgcac aacccatggc gactgcaaag ccattctccc
     1381 cgcagatgat cgccgcacgc aaggcaaaag agcagattgt ttacaccggt tttgcagccc
     1441 aggaggtgga gcaggcggct caatcgctgc actacaattt cagtggcgtc gatgcggcca
     1501 ggaacgataa ggatctgtac agcctgcgct atggagcgtt tgtggtgcca ttgacaaaag
     1561 cagttcagga gctttccgcc cggaacgacc ggttggaaaa agaaaatgaa ttactgacaa
     1621 aacaaagcca acagattagg aaaaggattg cgatactaaa gcagaaaatg aattgatttt
     1681 tacaccgatc ttttacataa tgaaaaaggt actcattgca actgccgctt ttgttttgat
     1741 gctgttcagc acgaagaatg cccgggcgca gttcactatt gccggaggag ctcaattttc
     1801 catgtccggc aatctgcagc taaccctttc caatacggac ctggtcaata acggaacgct
     1861 ttccgctgga acaggcacca tttcctttac cggcaatgcc ccttcgggta ttcttggcag
     1921 tcagcctacc caattctacg agctgaagat cagcaaagac gccggaaatt ccgttacgct
     1981 gcaaaggccc atcggtgttg ggcggcaaat tactttcatt tccggcttcc tggatctcaa
     2041 cggttatgat gccgacctcg gcaacacggg cctgctgaac ggcgaacagg aatccagccg
     2101 cgtcactggc gccaacggag gagagctgat cttcaatggc ccgctcaatg cccctgccgc
     2161 cgtcaacccc ggcaacctcg gtatcctgat cagctcatcg caaaacctgg gcaataccac
     2221 cataaaacgc ggtcatcagt cgcagaccag cagcggtggt accggaagtt ccatactcag
     2281 gtattacgat attgtgccgg ccaataatag cggcccgaac gccaccctgc gtttccagta
     2341 ctttgaagga gaactcaacg ggctggatga aaacgcgttg atcttatggg agaaaaccgc
     2401 tgcgcaaccc tggactgccc tggggtttga cagccggaat atcaacccgc actacgtgga
     2461 aaagacgggt gtttcctctt ttgaccggtt caccctgtcc gcccccggcg atccgctgcc
     2521 cgtaattttt aatttcttca gggcccgttg caatggaaac caggtggagc taacctggca
     2581 gacagcccag gaggagaaca gccattattt tacagcggaa agaactgctg atggcaacac
     2641 ctggattccg ctgggcaata tccccgcggc cggcaactcc ccgaatgaac acgactattc
     2701 ctttacagat caggcgccca tccaaaaggg ccaataccgc attgccgaat atgatatgga
     2761 cggccgtgta cagtatacca aaatagcagc ccccggttgc agcatcctgg atacattccg
     2821 cgtctggccc aacccggtgc gcggaaccct ttatatcaat atcactgccg ataccgtttc
     2881 agcagccata ataaaactct tcgacagcaa gggcgccacg ataaaacggc agaccggtat
     2941 tctgtttccc gggaataatc tgctgaccct ggatctgaga gctgttgccc aaggggtgta
     3001 ctttttaagc atcatacgta ataacgggca ggtgcaggtg cgggagctga taaaagaata
     3061 attgttggcc ggcgcgctta gctttttggt tcgtaaacaa gcaatacggt tccgcaggca
     3121 aaggtcctgg attccagaag ggtcagaaca agctttccct ttaagctttt gaatatcgtt
     3181 tctccattga tcagtgcaac cggatttaag agaaaataat attggtcaat gagcccttct
     3241 ttaatcagcg acgatacaaa actgcttccg ccataaacaa tcaggtctcc gcccgcccgt
     3301 ttttttagtc tttgtacttc ttcaaccgtt tcccctcttg ccaattccgt atttggccag
     3361 ctggatttgg ccagcgtctt tgaaaaaaca attttcggaa tctccgttaa ccgctttcca
     3421 atttcataat ccggatcatc cggcttggcg gatatcgaag cccagtgcgg tacaaagcca
     3481 ttggccgtat ttcgaccaag gatgatccgg tcaactccgt ctatattggc caggacaaaa
     3541 gacttcacct cgctatccca actgaaattc gctcctcccc tatccgtagt cacgcaatcc
     3601 agggatatct gcatctgaag ttttaatttc cccatataaa tattgattta aattgttcac
     3661 acaaatctac aggctgcaga tttcctgatg agggtgtgaa aatgacatta tagggggctg
     3721 atcgcgacaa cccgaagctt ttacttatcc gccggttcat atttcagaaa atctccgtca
     3781 atgatcagca gcttaactcc gttttgtgaa atggaacggt gttcactccg ttcatcggat
     3841 acaatacagg tttcgttcct ggagaatttc attctttctc cggtgcgctg ttcgctgatg
     3901 aattcacctt cgaggcaatg cacgatatgg cctttccggc accagtcatc cgccagatac
     3961 ccgcttgaat attccaccag gcggacccgg aggccgtcca actgaatggt ctgccagaag
     4021 gccgatccgg tttccccttt atattcaacc ttttcaattg atgtccagtc aatagtctgg
     4081 aatggtatat tcttcatttt tgtaatggtt cgttaacttc aaatttattg aatatccgga
     4141 atgaacattc aaacgctttc gacgtatttt gcattcaaaa taaaatcgca tgttcacgat
     4201 cgaacaaata aaaacagcgc acgcaaacgt aaagacagga gcggattatc cgcggtatgt
     4261 tcaggaaatt atcgccttgg gcgtaaccgg ctatgaaacc tgggtcggcg acggaaatac
     4321 gatctatttt ggagccggaa acatcctggt ggttgaatcc atcgccaaac catgagaaag
     4381 gaattgcccg taaattttac tttacccttt atgcaaacag taagcaaatc atttcaaacc
     4441 ggcagatcaa ttcagaccag cacgctgatc ctggcccttt tgctcgtccc ttttttttcc
     4501 agcgcccaaa aaaaactgcg gaagataacc acgccggaag cctggtcgca ggactatccg
     4561 cc
//
LOCUS       DASYCU010000042         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4361172, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000042 DASYCU010000000
VERSION     DASYCU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4361172"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VGM24_00860"
     CDS             complement(<1..427)
                     /locus_tag="VGM24_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153936.1"
                     /translation="MLNNLKPTFRQLAFHHDASLHVKIDSGENLRNSLHYHPEIELIL
                     LKRSVGMRIIGNSFESFKHKDVFLIGKNTPHRFLHEEDYLLNHFDTPEAIVVQFDDTF
                     LGKEFLALPEFKEIQQLFTIAKLGVSLTEQGKTEVIPLIE"
     gene            891..>3011
                     /locus_tag="VGM24_00865"
     CDS             891..>3011
                     /locus_tag="VGM24_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153937.1"
                     /translation="MIKKIVAPLIFQRWDNGNRLLQVGRRRAGNYHHLNERRVSLLAF
                     AILGFFLTASFSAFSQSENRMPFPAAPLTEKGDLSALMVSGIDQFLSAETQRQRAGRD
                     QLWHRDFSSAGAFNQSISAQRQLLSERLGVVDQRVRPKLELMTNDQLQPFVAATTTCT
                     IQAVRWEVFDGLSAEGLLIRPKGKITARIVLIPDADGTPEVLAGLHKGGPGYDIGRRL
                     AASGWEVLIPTLVSRADTFSGNPSLGLSTNLTHREWIYRQGYEVGRHVIGYELQKIFS
                     GIDWFTLRNGAEGKALPIGIAGYGEGGLLALYAAALDTRISASLVSGYFNQREQLWRE
                     PIYHNVFGLLTNFGDAELAVMAWPRALVVEQSKAPEIAGPPLASRRRSGAAPGSLATP
                     AYATARDEWARAKEMEPKGNENVRWCSNGNTDFKAPFSEAALAGLASGLHLKTPESHS
                     LPLPLKQPQVWVDPIRRQERTVREMEQTVQSVLTLSERTREEKFWAMLKGDTAAQKPI
                     KAVFRAKLWDQLGQLPTPSMPANPRARLLQQTAKWTSYEVMLDVWPGVFAWGILMVPN
                     DIKPGERRPVVVCQHGLESVPMDVVTTDSTAKNFHFYRGFASRLADRGYIVFAPCNLY
                     IGGNKFRVLQRKANPLGLTLFSVMIGQHQRILQWLKGLSFADPDRIGFYGLSYGGKSA
                     MRIPAVVQGYALSICSGDFNEWIRK"
BASE COUNT          770 a          729 c          751 g          761 t
ORIGIN      
        1 tttctatcag gggaatcact tcagttttac cttgttccgt caggctgact cccagttttg
       61 caatcgtaaa cagctgttgg atttctttaa attcaggaag agcaaggaac tcttttccaa
      121 gaaaagtatc atcaaattga acgactatcg cttccggggt atcaaaatgg ttcagcaaat
      181 agtcctcttc atgaaggaaa cggtgaggcg tgtttttacc tatgagaaat acatctttat
      241 gcttaaagct ttcgaaactg tttccgataa ttctcatacc tacggacctt ttaagcagga
      301 tgagttctat ttcaggatga taatgcaatg agtttcgtaa attctcaccg gaatcaattt
      361 tgacatggag tgaagcatca tgatggaatg ccaattgacg aaacgttggt tttaagttat
      421 tgagcataca ggtatggata attttaacat atagaaatcg attgtaattt ataaaaaatt
      481 atgcaattac aaatttaatc tgcagcgctt cttcccagaa cattttgtac tatagcctgt
      541 atcaattgac gttttaagtc gctttcatca tatggctccc cgactttttt aggtttaatt
      601 tatttttgaa aatcgatttc caaattgtgt ttttagttag cctggatggc agaaacagcc
      661 tccaaattaa atcctgttac tgaaattatg ggcacggccg ggcgcgcagg ttgcccgtat
      721 gagccggaat ataacgcggc cctgaccggg caaccttttt ttaacggggc aattgtcagg
      781 ataaaatatg tgagcgatac gctaatattt agtaataaaa acaagacaaa acacggttat
      841 ttgcattcct gattctataa ccagttcaaa cttgataaaa agtaaatagc atgatcaaaa
      901 aaattgttgc tccgcttata ttccagcgat gggataatgg taaccgcctg ctccaggtcg
      961 gccgaagaag agccggaaat taccatcacc taaatgaacg gcgggtatcg ttattggctt
     1021 ttgcgatttt aggattcttc ctcaccgcat ccttttctgc attctcccaa tcagaaaaca
     1081 gaatgccttt tcccgcagct cctttaactg agaagggtga tctttctgcg ttgatggtat
     1141 ccggaatcga ccagttcctc agcgcggaaa cccagcgcca aagggccggc agggatcagc
     1201 tctggcaccg cgacttttca agtgccggcg ccttcaatca atctatttcc gcacaacgcc
     1261 agctgctgtc agaacgcctt ggtgtagttg accagcgggt caggccaaag ctcgaactga
     1321 tgacaaatga tcagttacaa ccttttgttg cagccacgac aacctgcacc atccaggcgg
     1381 ttcgctggga ggtgttcgac ggattgtcgg cggagggcct gctgatcagg ccaaaaggaa
     1441 aaataacagc ccgtatcgtg cttataccgg atgccgacgg gacgcccgaa gtccttgccg
     1501 gtttgcacaa aggcgggccg ggatatgata taggacggcg gcttgccgca tccggctggg
     1561 aagtcttgat tccgacgctg gtcagccggg ccgatacctt ttccggaaac ccttcccttg
     1621 gcctttccac caacctgact caccgcgaat ggatctaccg gcaggggtac gaggtcggtc
     1681 gtcacgtcat cggctatgaa ctgcaaaaaa tattttccgg catcgattgg tttacactga
     1741 ggaacggggc tgaaggaaag gcattgccca tcggcatagc aggttatgga gaaggcggtt
     1801 tactggcgct gtatgcagct gctctggata cccgtatttc cgccagcctg gtaagcggct
     1861 attttaacca aagagaacaa ctctggaggg agccgattta ccataatgtt tttggattac
     1921 tgacgaattt cggagatgca gaactcgccg taatggcctg gccacgggcc ttagtggtcg
     1981 aacaatcgaa ggcccctgaa atagcggggc cgccccttgc ttcccggaga cgatctggcg
     2041 ctgctcccgg cagcctggca acgcctgctt atgcaacggc acgggatgaa tgggcacggg
     2101 caaaggagat ggaaccgaag ggcaatgaaa atgtgcgctg gtgctccaat gggaataccg
     2161 attttaaagc acctttttcg gaggccgccc ttgccggcct ggcttccggg cttcacctga
     2221 aaacgccgga gagccattcg cttcctctgc cgttaaagca accgcaggta tgggtcgatc
     2281 ccatcaggag acaggagcgt accgtgcgtg aaatggagca aaccgtgcaa agcgtcctca
     2341 ccctgtctga aagaacacgc gaagaaaaat tctgggcaat gctgaaaggc gatactgctg
     2401 cacaaaagcc gataaaagcg gtcttcagag caaaactctg ggaccagctc ggccaattac
     2461 ctaccccctc catgccggct aatccgagag cccgcctgct tcaacagacg gctaaatgga
     2521 ccagctatga agtcatgctc gatgtgtggc cgggggtatt tgcctggggg atactgatgg
     2581 ttccaaacga tatcaaaccc ggggagcgcc gtccggtcgt agtttgccag catggcctgg
     2641 aaagcgtacc tatggatgtc gtcaccaccg attccaccgc aaagaacttt cacttttacc
     2701 ggggttttgc ctcccggctg gccgaccgtg gctatatcgt gttcgcaccc tgcaacctgt
     2761 atattggcgg gaataaattc agggtgcttc aacggaaggc caaccccctg ggcttaacct
     2821 tattctctgt gatgatcgga cagcatcaac ggatcctcca atggctgaaa ggactttcat
     2881 ttgcagatcc tgatcgtatc ggattttacg gcttatccta tggcggaaaa tcggctatgc
     2941 ggataccggc agtggtacag ggttacgcgc tctcgatttg ttccggtgat tttaatgagt
     3001 ggatacgcaa a
//
LOCUS       DASYCU010000043         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2186354, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000043 DASYCU010000000
VERSION     DASYCU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186354"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VGM24_00870"
     CDS             <1..1080
                     /locus_tag="VGM24_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY4153938.1"
                     /translation="IRGDKGLDYENWCLTSSEFGKQAILSDLLYFKYYFLDTLQLPYD
                     KEKLVADFEIFASDLSKVDYKYFMFRDFQSRNIMIENEKVHFIDYQGGMKGALQYDVA
                     SMLWQAKAELPDEWKNKLLEYYMDCVESELGRPVDRERFIRQFRGYVLIRLMQVLGAY
                     GFRGLFERKAHFLISIPLGLKNLRDFLQNHFTGRGLSEYERVLHLIVEDQVIQRFVPL
                     RASAETPLQVHINSFSYKKGIPADSSGNGGGFVFDCRSILNPGRLDAFKSKTGRDKEV
                     KDFLEQETKMPEYLNSVFDLVDISVEDYINRGFKSLMVNFGCTGGQHRSVYAADALAR
                     HLKNKFGVNIGIHHVEQELKNWENG"
     gene            complement(1098..1862)
                     /locus_tag="VGM24_00875"
     CDS             complement(1098..1862)
                     /locus_tag="VGM24_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY4153939.1"
                     /translation="MKAMIFAAGLGTRFKPWTEKHPKALAPINGKSLLERNIRYLQQF
                     GITEVVLNVHHFADQIMEAIAKNEAWGSRIAISEEIGEVLETGGGLKKASWFFTDGPF
                     VVMNADILTDLDLNNMIAFHRQHTPLATLAVTNRKSSRYFLFNEKNRLCGWRNIKTGE
                     ERLVISGPGVPPVKSGDEPAQGSLPVLNEKAFSGIHIISPDLFSFLHREGKFSIVDVY
                     LELAGRKPILEFDHSGSKLVDVGKPEAVAVAERMFI"
     gene            complement(1911..3512)
                     /gene="nadB"
                     /locus_tag="VGM24_00880"
     CDS             complement(1911..3512)
                     /gene="nadB"
                     /locus_tag="VGM24_00880"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY4153940.1"
                     /translation="MIRKTDFLVVGSGIAGLTYALKVARACPGQQVLVITKTIADETN
                     TKYAQGGIAGVTDLEHDSYEKHIEDTLIAGDGLCNPRTVEIVVKEGPERIREMIEWGA
                     RFDKDEEGEFKRGKEGGHSEFRILHHKDVTGREMERALLNTVSAQKNIEIIKHYFVVD
                     ILTQHHLGYLVTKATPDIECYGVYALDLRENRIVKILSRLTVFATGGNGQVYRTTTNP
                     VIATGDGIGMVYRAKGRIENMEFIQFHPTALYQQGVSPSFLITEAVRGDGGILRNQNE
                     EAFMARYDERKDLAPRDIVARAIDNEMKKAGTEHVYLDCRQMDIEKFRDHFPNIYEKC
                     KSIGIDVARQMIPVAPAAHYSCGGIKTDEWGRTSIGNLYACGECASTGLHGANRLASN
                     SLLEALVFAHRCYLDSVQKIKTISFNEKIPDWNAEGTTAPNEMILITQSIKELQLLMS
                     DYVGIVRTNIRLERAMKRLDLLHEEIEALYEATVVSPQLCEVRNLITTGYLIVKAAQF
                     RKESRGLHYNTDYPQKSKLVQNIVL"
     gene            complement(3528..>4192)
                     /locus_tag="VGM24_00885"
     CDS             complement(3528..>4192)
                     /locus_tag="VGM24_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153941.1"
                     /translation="TVNTDMSQLLEAMQNYLGKEEMEKQMPAGKKMDTTVLMKNLVDS
                     MKDVTPESKELVKDGKVHMQLDLDQKIFKSDIQIPFNSQDNLQRLYNTMNNQNLGFTQ
                     LFKGMSRNPDSAGNNNVPEMNQFNAIYDFQSHDGLLSRKLNQQKWKDLQQSPQFGQMK
                     DAGSMGISIPYTLTINLPRPVKKIDNALAKLSADKKQVVIQYNLIEVFDHPEKFEYTI
                     VY"
BASE COUNT          943 a         1022 c         1059 g         1168 t
ORIGIN      
        1 atccgcggag ataaaggact tgactatgag aactggtgcc tcacctccag cgaattcgga
       61 aagcaggcca tactcagtga cctgctgtat ttcaaatatt attttctgga tacccttcaa
      121 ctgccgtacg acaaggaaaa gctcgttgcg gatttcgaga tctttgcctc ggatctcagc
      181 aaggtggact ataaatattt tatgttccgc gattttcaaa gccggaatat catgatcgaa
      241 aatgaaaaag ttcattttat cgattaccag ggcgggatga aaggcgcgtt gcaatatgat
      301 gtggcttcca tgctgtggca ggccaaggcc gagttgccgg atgaatggaa gaacaaactg
      361 ctggaatatt atatggattg tgtggaatcg gaactgggaa ggcccgttga ccgggaacgt
      421 ttcatccggc agtttcgggg atatgttctg atccggctga tgcaggtgct gggcgcttat
      481 ggattccgcg ggttgtttga gcgcaaagcg catttcctga tcagcattcc gctgggatta
      541 aaaaacctgc gtgatttttt gcaaaaccat ttcaccggcc ggggtctatc cgaatacgaa
      601 agggtgctgc atctgatcgt ggaagaccag gtgatccaac gctttgtacc gctgcgcgcc
      661 agtgcggaaa ctccgttgca ggttcacatt aacagttttt cctacaagaa gggcattcca
      721 gccgattcgt cgggtaacgg cggcggattc gtatttgatt gccgcagcat tttaaatccc
      781 ggaagactgg atgcatttaa gtcaaagacg ggccgggata aggaagtaaa agattttctt
      841 gaacaggaaa ctaaaatgcc cgaatacctc aacagcgttt ttgaccttgt ggatatatcc
      901 gtggaggact atatcaaccg cggatttaag agtctgatgg taaattttgg ctgcacgggc
      961 ggccagcacc gcagtgttta tgctgccgat gcgctggcca ggcaccttaa aaataagttc
     1021 ggagtgaaca tcgggatcca tcatgtggag caggaattga agaactggga aaacggatga
     1081 gtccgtcgcc gggataatta aatgaacata cgctccgcca ccgcaacggc ctccggtttc
     1141 ccgacatcca ccagtttcga accggaatgg tcaaattcaa gtatgggctt ccggccggcc
     1201 agttccaggt aaacatcaac aattgaaaac tttccttccc ggtgaaggaa ggaaaacagg
     1261 tccggtgaga taatatggat cccgctgaat gctttttcat tcaataccgg cagggaaccc
     1321 tgagcgggtt catcaccgga ttttacaggt ggcactccgg gaccggaaat aaccaggcgt
     1381 tcttccccgg tttttatatt ccgccagccg cagagccggt ttttttcatt aaaaagaaag
     1441 taacgggacg atttgcggtt tgtcacggcc agcgtggcca gcggcgtgtg ctgccggtgg
     1501 aaagcgatca tgttgttcag atccaggtcg gtcagtatat cagcattcat gaccacaaac
     1561 gggccgtccg taaaaaacca ggatgctttt tttaacccgc cgcccgtttc cagcacttcc
     1621 ccgatctcct cactgatcgc gatccggctg ccccaggctt cgttctttgc tattgcttcc
     1681 attatctggt ctgcgaaatg atgcacgttc agcacgactt cggtaatgcc aaattgctgc
     1741 agataacgga tgttccgctc cagaagactt ttcccgttaa tgggcgcaag cgctttggga
     1801 tgcttttcgg tccagggttt gaaccgtgtt cccagccctg cggcaaagat catggctttc
     1861 ataaacgaaa attatcttaa tgggttcggc tcaggcaaaa cctcggttta tcagagaacg
     1921 atattctgta ccagtttgct tttctgcgga tagtcggtgt tgtaatgcag accgcggctt
     1981 tctttccgga actgtgcggc cttgacaatc aggtagccgg tggtgatcag gttcctgact
     2041 tcacatagtt gcggggagac caccgtggct tcatacaatg cttctatttc ttcgtgcagc
     2101 aggtcgagtc ttttcatagc cctttccaaa cggatattgg tccggacaat gcctacatag
     2161 tcgctcatca gcagctgcag ctctttaatg ctttgcgtga tcaggatcat ttcgtttggc
     2221 gcggtggtgc cttccgcgtt ccagtccggg attttttcgt tgaaagaaat agtttttatt
     2281 ttctggacgc tgtccaggta acagcgatgc gcaaagacca gtgcttccag cagtgaattg
     2341 ctggccagcc ggtttgcccc gtgaagcccg gtggatgcac attctccgca ggcatagagg
     2401 ttgccgatgg aggtacggcc ccattcgtcc gtcttaattc cgccgcagct gtaatgggcc
     2461 gccggcgcaa cggggatcat ttgtctggcc acgtcaatgc cgatggattt acatttctca
     2521 taaatattcg ggaagtggtc ccggaatttt tcaatatcca tctggcggca gtccaggtat
     2581 acatgctcgg ttcccgcttt tttcatttca ttgtcgatag cgcgggctac gatatcgcgg
     2641 ggcgcaaggt ctttccgctc gtcgtacctg gccataaaag cttcttcgtt ctgatttcgc
     2701 aaaatgcctc cgtcgccgcg caccgcctcg gtgatcagga aggaaggact tacgccctgc
     2761 tggtacaggg ccgtgggatg aaactggatg aactccatat tctcaatcct tcctttagcc
     2821 cggtatacca tgccgatgcc gtcgccggta gcgatgacgg gattggtggt ggtgcgatat
     2881 acctgtccgt ttcccccggt ggcaaaaacg gtaagccggg ataagatttt tacgatgcgg
     2941 ttttcccgca gatccagcgc atatacgcca taacattcga tatcgggcgt ggctttggta
     3001 acgagatatc caagatgatg ctgggtgagg atatccacca caaagtaatg cttgatgatc
     3061 tcgatatttt tttgggctga caccgtattc aggagagccc gttccatttc tcttccggtt
     3121 acatctttat ggtgcaggat ccggaactcc gaatggcccc cttccttccc gcgtttaaat
     3181 tcaccttcct catccttgtc aaaccgggcg ccccattcta tcatttcccg gatgcgctcg
     3241 ggtccttctt tcaccacgat ctccacggtt ctgggattgc agagcccgtc cccggcaatc
     3301 agcgtgtctt caatatgttt ttcgtaactg tcgtgctcga gatccgttac gccggcaatc
     3361 ccaccctgtg catatttggt attggtttcg tcggcaatgg ttttggtgat caccagcacc
     3421 tgctggccgg ggcaggccct ggctactttc aatgcatacg tgagtccggc aatacccgaa
     3481 ccaacaacca gaaaatccgt ttttcttatc atagtcggaa actgttccta ataaacgatc
     3541 gtgtattcga atttttcagg atgatcaaac acttctatga gattgtattg gatcactacc
     3601 tgttttttat cggcggataa tttggcaagt gcgttatcta ttttttttac cggccggggt
     3661 aagttgatcg tcagcgtgta aggaatggaa atgcccatgc ttccggcatc tttcatttga
     3721 ccgaactggg gactctgctg aaggtccttc catttttgtt gatttagttt gcgggagagg
     3781 agtccgtcat gactttgaaa atcataaatg gcattgaact gattcatctc cgggacatta
     3841 ttgttaccgg cggaatccgg attacgcgac atgcctttga acaattgggt aaaaccaagg
     3901 ttctgattat tcatcgtatt ataaaggcgc tgaaggttat cctgactgtt gaagggtatt
     3961 tgaatatcgc ttttgaagat cttctggtcc aggtcgagtt gcatgtgtac tttgccgtcc
     4021 tttaccagct ctttgctttc gggtgtaacg tctttcatgc tgtcgaccag gtttttcatt
     4081 aacaccgtag tgtccatttt tttacctgca ggcatctgtt tttccatttc ctcctttccc
     4141 aggtaattct gcatggcctc gagcaactgg ctcatgtcgg tattcaccgt ca
//
LOCUS       DASYCU010000044         7977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_157593, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000044 DASYCU010000000
VERSION     DASYCU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157593"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1495
                     /locus_tag="VGM24_00890"
     CDS             74..1495
                     /locus_tag="VGM24_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY4153942.1"
                     /translation="MRKLVLFEWLLLVALITAGISCRTGKNYQRPEVPLPDRFGQQSS
                     YSDTSSIADIDWKTFFADTALQQLIDSGISRNYDLQLALKRIGIAQEQMKQARLLWYP
                     QVDLQISGQVNRPSDNSLNGISANSFLGKSYIEDYNASLNMTWEIDIWGKISRQKEST
                     LAQYLQSYEGAKAVQTRLVADIAQGYFNLLMLDQQLAVSRQNLELNENFEKITRLLFD
                     AGEVTYLAVQQAVSQKQSTALLVPQLEQSIAIQENALQLLTGSLPGAIARQERLSDMH
                     FRDSLSAGLPVAMLSRRPDVRSSEMALVYANAQVGIAQADMYPAINLTAGVGLESFKA
                     SNWFNIPNSLFGLATGTILQPLFRRRELKTRFETAKLEREEAVIQFRQSVLTATTEVS
                     NAQVQIDKLKEQEGIARAQTDTLKSAVSNAEYLFKSDMANYLEVITAQQNALQSQLNL
                     ASIQRQKLDAMVELYRALGGGWK"
     gene            complement(1497..2294)
                     /locus_tag="VGM24_00895"
     CDS             complement(1497..2294)
                     /locus_tag="VGM24_00895"
                     /inference="COORDINATES: protein motif:HMM:NF025037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C/glycerophosphodiester phosphodiesterase family protein"
                     /protein_id="HEY4153943.1"
                     /translation="MTPFFLRFRGLLITCALYFIGANANAQPAHYSISNTHSHNDYEQ
                     AHPFSTAHQAQFGSIEADIFLVNNKLLVAHSPSELSLNRSLEGLYLKPVLQALSENGN
                     FPYPDTSRQLQMLIDIKTDSVKTLDALISLLKKYPDLTRSPKLKWVISGNRPAPDHFD
                     SYPDFIWFDGELKRTYSEKALSRILMMSDNLARYTRWKGKEPLPENDRLLLEQIISKA
                     HRLGKTVRFWNAPDFKEAWETLISLGVDYINTDHIEALSQFLNSRKR"
     gene            2402..4021
                     /locus_tag="VGM24_00900"
     CDS             2402..4021
                     /locus_tag="VGM24_00900"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY4153944.1"
                     /translation="MNRRNFLRTGSLTGVGLTAAGLSSFQAPSRRLASPSATEHFPAI
                     ELEEITIAGLQKKMQQHQATSVSITKAYLDRIQKMDKEGPHLNAIIEINPDAISIAEG
                     LDRERNQGKIRSALHGIPVLVKDNINTGDRMMTTAGSLALSGNIAREDAFIVRQLRNA
                     GMVLLGKTNLSEWANFRSSRSASGWSSRGGQTKCPYILDRNPSGSSAGSGSATASNLC
                     AVSVGTETDGSIVSPASVNGLVGIKPTVGLWSRSGIIPISATQDTAGPMARTVTDAVI
                     LLGAGTGADPKDPITSESTGKALQDYTSFLIRDGLKGKRIGIEKSHLKENPDLVALLR
                     NAMRLLESQGATVVEVDVQSELDKLGHLEYQVLLYEFKDGLNRYLASSTSGVKSLKEL
                     IAFNLANEPRAMPFFKQEILMESEEKGNLETREYRDALSKTWGFRKTITDLMSANKLD
                     ALSGITNGAACCIDLVNGDYGNGPSLSTPAAITGFPHITVPMGLVHGLPIGISFYGEA
                     YSEPALISMAYAYEQASNQRRKPVFQKDLLG"
     gene            complement(4026..5567)
                     /locus_tag="VGM24_00905"
     CDS             complement(4026..5567)
                     /locus_tag="VGM24_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153945.1"
                     /translation="MSLGNWQQLTGKGNHWTFQDISVMLDQKGQETAVQLTSPGREIE
                     KIRIGWKHHSPSGAAFLGDHWERSYGDLAWKPAAAERKMPWYMLIHNGRETAAFGVKT
                     GAGSICYWQAGPEQIELFLDTCSGGMGVMLGDRVLSAAEIISTKSLPGETPFQTDARF
                     CKMMCPKPVLPEKPVYGINDWYFAYGNNSKELILRITGLMADLAGNASNPPFSVIDDG
                     WSLKSARGNEVCCWGDDYTRSNSTFGDMAVVAADIKKLGMRPGLWTRPLLANSSDPLT
                     ALTPLRKGQTNEKDRYLDPTIPENLQRIRHTFRLYGDWGFEMVKHDYSTYDFFGRWGF
                     EMNEEMTSPGWHFNDRSGTNAEIILTLYRTIREAAGRMYLIGCNTVSHLSAGLFELNR
                     IGDDTSGKEWARTVKMGVNTLGFRLPQHNAFYAADGDCVGLTTQIPWKENKQWLQLLA
                     ESSAPLFISAQPEALGAAQKEAIRKSLSMASGYQPLGEPLDWLANPRPAAWKLNGRTV
                     NFDWA"
     gene            complement(5760..7013)
                     /locus_tag="VGM24_00910"
     CDS             complement(5760..7013)
                     /locus_tag="VGM24_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY4153946.1"
                     /translation="MKLKGRFRNYLKILGPGMITGASDDDPSGIATYSQVGAAFGLQL
                     LWLAPFTFPLMASLQGMCARIGIVTTHGLTNTLKKFYPPAILYLMLFFSIPAIILNIG
                     SDIAGMGAVSNLLVPAVPPAVFSVFFTILLMVFIVLFSYQKIARVLKWLCLAMLLYLL
                     VPFIVNVDWKEVLFYTFVPHIRFNKAFFEMIVAILGTTISPYLFFWQATMEAEDVNHK
                     VTKVIVDKRILSDMAADVNTGMFASNLVMLFIILTTGVVLHNQGILQVDTVDQAAGAL
                     APLAGKLSYLCFALGVIGTGFLAIPVLAGSLSYILAESFGWQQGLDKKFHEARGFYIA
                     IILSLAVGLLINFTGIPAMKALLYTAILYGVTSPVLIAVVLHICNNRKIMQDHTNSRL
                     SNILGFITLLVMAAGALALLYFQFV"
     gene            complement(7119..>7977)
                     /locus_tag="VGM24_00915"
     CDS             complement(7119..>7977)
                     /locus_tag="VGM24_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY4153947.1"
                     /translation="LFDPEKYFLVCVNMPGSCYGSIGPLENNPETGQPYYHDFPFFTT
                     RDMIRAYQPLREFLGIKKIKIGIGGSMGGQQLLEWAIEEPDLFEHIVPIATNAIHSPW
                     GKAFNASQRLCIELDPEWLQRHPLAGVQGMKVARGIALLSYRHPETYDLYQGDPDINS
                     LGPYRSESYQVYQGEKLARRFNAFSYYRLSQSMDSHHVGRGRGSILQALKRIRAKTHV
                     VSIHSDLLFPQKEQEFLAANIPGASYFVIPSLFGHDGFLIESEALSVIINDCIKTEDL
                     SPRSSYSPG"
BASE COUNT         2028 a         2001 c         2094 g         1854 t
ORIGIN      
        1 taaggagcat aagaaaagga atcgtttccg ctgaatcgcg ggttacggag aaaaaaaatt
       61 taaacgttca gggatgagaa aacttgtttt gttcgaatgg ctgctcctgg tcgcgctgat
      121 tacggccgga atttcctgca ggacgggaaa aaattatcag cggccggaag tgcccctgcc
      181 ggaccggttc ggtcagcaat ccagttattc ggataccagc agtattgcgg atatcgattg
      241 gaaaactttc tttgccgata cggcgcttca gcaactgata gacagcggga tcagccgcaa
      301 ttacgatttg cagctggcgc tgaaaagaat cggcatcgca caggaacaga tgaagcaggc
      361 caggctgttg tggtacccgc aagtcgatct gcagataagc ggacaggtaa accggccgtc
      421 tgataacagc ctgaacggga tcagtgcaaa tagttttctg ggaaaaagtt atatcgagga
      481 ttataatgca tcgctgaata tgacctggga aatagatatc tggggaaaga tcagtcgcca
      541 gaaagaatct accctggcgc agtatctcca gtcctacgaa ggcgcaaaag cggttcagac
      601 caggctggtg gcagatatcg cccaggggta ttttaatctc ctgatgctgg atcagcagct
      661 tgcggtctcc aggcaaaacc tggaactcaa tgaaaacttc gagaagatca cccgtctgct
      721 ttttgatgca ggcgaggtca cctatctcgc cgttcaacag gccgtatccc agaaacaatc
      781 caccgctctg ctggtccccc agctggagca gagcatcgcc atacaggaga atgcgctgca
      841 attgcttacg ggaagccttc ccggcgctat cgcgcggcag gagcgcctga gcgacatgca
      901 tttccgggat tccctgtctg ccggcctgcc ggtggccatg ctgagccgga ggcccgatgt
      961 acgttccagc gaaatggcgc tggtatatgc caatgcccag gtgggtatcg cgcaggcgga
     1021 tatgtatccg gcaattaatc tgacggccgg cgtgggcctg gagtctttta aagccagcaa
     1081 ctggtttaat attccgaatt ccctgttcgg actggccacc ggtacgatcc ttcaacccct
     1141 gttcagacga cgggagctga aaacccggtt tgaaaccgcg aagctggaaa gagaagaagc
     1201 ggtcatccag ttccggcaat ccgtcctgac ggccaccacc gaagtgagca atgcccaggt
     1261 gcaaattgat aaattgaagg aacaggaagg gattgcccgc gcacagaccg acaccctgaa
     1321 gtcggccgtt tccaacgcgg aatatttgtt caaatccgac atggccaatt acctggaggt
     1381 gatcacggcc cagcagaacg cattgcagtc ccagttaaac ctcgcttcga ttcagcggca
     1441 gaaactcgat gccatggttg aattatacag ggccctgggg ggagggtgga aataagttat
     1501 cgtttacggg agttcaaaaa ttgggaaagg gcttcaatat gatcggtgtt aatataatca
     1561 acacccaggc tgatcagggt ttcccaggct tctttaaaat ccggggcatt ccagaacctg
     1621 accgtttttc caagccggtg tgcttttgaa atgatttgtt ccagcagtaa gcgatcgttt
     1681 tccgggagcg gttcttttcc cttccagcgg gtgtaccgcg ccaggttgtc gctcatcatc
     1741 aggatccggg acagtgcttt ttcagaatag gtcctcttca attctccatc gaaccagatg
     1801 aaatcgggat aactgtcaaa atggtctggg gccggccggt ttccggaaat cacccatttt
     1861 agttttggag aacgggtcag gtccggatat tttttcaata aagaaattaa ggcatccagc
     1921 gtttttacgg aatcggtttt tatatcaata agcatttgca gctgccggct ggtatccgga
     1981 taaggaaaat ttccattttc ggacaatgcc tgcagaaccg gtttcaggta gaggccctcg
     2041 agcgaacgat tcaaagaaag ttccgaagga ctgtgtgcca cgagtaattt attattcacc
     2101 agaaaaatat ctgcttcgat ggatccgaat tgcgcctggt gagccgtgga gaacgggtgc
     2161 gcctgctcgt agtcgttgtg ggaatgcgta ttggagatac tgtaatgggc gggttgcgcg
     2221 tttgcgtttg ctccgatgaa atacagagcg caggtgatca gcaatccgcg aaaccgcagg
     2281 aaaaagggag tcattttctt taaaaaattg gatccggaga taaatataat gaaatgatct
     2341 ctgtttattt tttgttcccc ggactccagc ctttgtctta attttaaata aacaacggct
     2401 tatgaacagg agaaattttt tacgcaccgg ttccctgacc ggtgtgggat tgactgctgc
     2461 cggtctttct tcctttcagg cgccttcccg gcgtctggcg tctccttcgg cgacggaaca
     2521 tttcccggcc atcgagctgg aagaaattac catcgccggg ttacagaaaa aaatgcagca
     2581 gcaccaggcc acatccgttt ccattacgaa ggcctatctc gaccggattc aaaagatgga
     2641 taaagaaggg ccacacctga acgccatcat cgagatcaat cccgatgcca tatcgattgc
     2701 agaaggcctt gaccgggaaa gaaatcaggg aaagatcagg agtgcattgc atggcatccc
     2761 ggtactggta aaggataata taaataccgg ggaccggatg atgaccaccg ccggctcact
     2821 ggccctttcc gggaatatcg ccagggagga tgcttttatc gtccgtcagc tccggaatgc
     2881 gggaatggtc ctgctgggaa aaaccaacct gagcgaatgg gccaattttc gctcctccag
     2941 atcagccagc ggatggagca gtcgcggcgg ccaaacaaaa tgcccctata tcctggaccg
     3001 gaacccgtcg ggatccagtg ccggctccgg ttccgctacg gcatccaatc tctgtgcggt
     3061 aagtgtcggt accgaaaccg acggttccat tgtttcaccg gcatccgtaa acgggctggt
     3121 cgggatcaaa ccaaccgtgg gcttatggag ccggtcgggg attattccca tttccgcaac
     3181 acaggatacg gccggaccga tggcgagaac cgtcacggac gccgttattt tattaggcgc
     3241 gggcaccggg gcggatccga aggatcccat tacctcggaa agcaccggga aagcgttgca
     3301 ggattatacc tcttttttaa tccgggacgg actgaaggga aagcggatcg gtattgagaa
     3361 atcccatcta aaagaaaatc cggatctggt tgcgctgctg aggaacgcga tgcgcctgct
     3421 ggaatcccag ggggcaacgg ttgtagaagt ggacgtacag agtgaactgg ataaattggg
     3481 gcatctggaa taccaggttc ttttgtatga attcaaagac ggattaaacc gctacctcgc
     3541 ttcttcaaca tccggcgtaa agtcgttgaa ggaactcatc gcttttaacc tggcgaatga
     3601 accccgcgcc atgcctttct ttaaacagga aattctgatg gaaagtgaag aaaagggaaa
     3661 tctggaaacc cgggaatacc gggatgcgct ttccaaaaca tgggggtttc gcaaaaccat
     3721 tacagacctg atgtcggcca ataaactgga tgcgctttcc gggattacaa acggggctgc
     3781 ctgctgcatt gacctggtga acggcgacta tggcaacgga ccttccctgt caacacccgc
     3841 cgccattacc ggatttcctc atattaccgt tccgatggga ttggtacatg gattacctat
     3901 cggtatttct ttttatggag aagcctatag tgagccggca ttgatctcca tggcttacgc
     3961 ttatgagcag gcttccaatc aacgaagaaa acccgttttc caaaaagacc tgctcggcta
     4021 gcgcgtcagg cccagtcaaa attcaccgtc cttccgttta atttccaggc ggcaggtctt
     4081 ggattggcca gccagtcgag tggttccccc aggggctggt atccggaagc catcgaaaga
     4141 cttttcctga tggcttcttt ttgcgcggca cccagggctt cgggctgggc ggaaataaat
     4201 aaaggagcgc tgctttctgc cagcagttgc agccattgtt tattctcctt ccagggaatc
     4261 tgcgtggtga ggccaacaca gtcgccgtcg gcggcgtaaa aggcattgtg ttgcggaagc
     4321 cggaacccca gcgtattcac gcccattttt acggttcttg cccattcttt gccgctggta
     4381 tcgtcgccga tgcggttcag ttcaaacagt ccggcagaaa gatgactcac ggtattgcac
     4441 ccgataagat acatccggcc ggcggcttcg cggatggtcc ggtataacgt caatatgatt
     4501 tccgcattgg ttccggaccg gtcattgaaa tgccatcccg gggaagtcat ctcttcattc
     4561 atctcaaatc cccagcggcc aaaaaaatca taggtgctgt aatcgtgctt caccatttca
     4621 aatccccaat ccccgtaaag acggaaagta tggcggattc gctgaagatt ttcgggaatg
     4681 gttggatcaa gataccggtc tttttcattc gtctgaccct tacggagagg ggtaagcgcc
     4741 gtcaggggat cgctgctgtt tgccaggagc ggtcgggtcc agaggccggg ccgcatgccc
     4801 agctttttaa tgtcggcggc gaccacggcc atgtcgccaa atgttgaatt ggatcgggtg
     4861 taatcatcgc cccagcagca gacttcattt cctctggccg atttgagcga ccaaccgtca
     4921 tctataaccg aaaatggcgg attggaggca ttaccggcga gatcagccat caaaccggtt
     4981 atccgcagga tcagctcttt tgaattatta ccataggcga aataccagtc gttgattcca
     5041 tacaccggct tttccggaag gaccggtttg gggcacatca ttttgcaaaa acgggcatcg
     5101 gtctggaagg gggtttctcc gggcaaactt ttggtgctga tgatttccgc tgcggataaa
     5161 acgcggtctc ccagcataac gcccattcca ccggaacagg tatctaaaaa aagttcaatc
     5221 tgctccggtc ctgcctgcca gtagcagatg cttccggccc ctgttttgac gccgaaagca
     5281 gccgtctccc ggccattatg gatcagcata taccagggca ttttgcgttc ggctgcagcg
     5341 ggcttccagg cgaggtcgcc ataggatctt tcccagtgat cacccaggaa tgctgcgccg
     5401 gaaggggaat gatgtttcca tccaatcctt attttttcta tttcccggcc gggagatgtc
     5461 aactgtaccg ctgtttcctg tcccttctga tccagcatta cggagatatc ctgaaaggtc
     5521 cagtgattcc cctttcccgt taactgctgc caattcccca ggctcaatac ggagacttcg
     5581 tcgggatgaa tgagcgcctg gtatgcctga ccggaaagcg gatttatttt tgtacatagt
     5641 aaggaagcag cggaaaaacc ggtgagctgt cagaattttc ttctttgcat ggaatgaatt
     5701 tgaatccgga catgcaagat agggtgaatc cggttcatcg gaaacccatc cgtcactccc
     5761 tacacaaatt gaaaatagag cagtgccaat gctcccgcgg ccatcaccag cagcgtaatg
     5821 aagccgagta tgttcgacag ccggctattg gtgtgatcct gcattatttt cctgttgttg
     5881 cagatatgca atacaacagc aatcaggacc ggggaagtta ctccatacag gatggccgtg
     5941 tagagaagcg ctttcatagc cggtatgccg gtaaaattga tcagaagacc gaccgccagc
     6001 gacaggatga tggcaatata aaaccccctg gcttcatgaa attttttatc caggccctgt
     6061 tgccagccga aggattcggc caggatatag gataatgaac ctgccaggac cgggatggcc
     6121 agaaagccgg tgccgataac ccccagcgcg aagcagaggt aagatagctt tccagccagg
     6181 ggagccagcg cccccgcagc ctgatccacc gtatccacct ggagaatccc ctggttatgc
     6241 aatacgacgc cggttgtcag tatgataaat aacatcacca ggttcgacgc aaacatcccg
     6301 gtgttcacat cggcggccat atcgctgagg attcgcttat ccacaatcac ttttgtcact
     6361 ttatggttga cgtcctcggc ttccatcgtc gcctgccaga agaacagata aggagaaatc
     6421 gtggttccca atatggccac gatcatttca aagaaagcct tgttgaaacg gatatgcggt
     6481 acgaaagtat agaacaacac ttctttccag tccacgttta caataaaggg caccagcagg
     6541 taaagcagca tggccaaaca aagccacttg agcacacgcg ctattttctg ataagaaaaa
     6601 agtacaatga agaccatcag taagatcgtg aaaaacacac tgaaaacggc tggcggcacc
     6661 gccggaacaa ggagattgct caccgctccc attccggcga tatcggatcc gatatttaat
     6721 atgatggccg gaatactgaa gaacagcatc aggtaaagga tagccggagg atagaatttt
     6781 ttcagcgtgt tcgtcagacc gtgcgtggtt acgattccga tccttgcaca cattccctgc
     6841 agggaagcca tgagcggaaa ggtaaaaggg gccagccaca ataactgcag accaaatgcg
     6901 gcgccgacct gcgagtaggt ggcaataccg gatggatcat catcgctggc gccggtgatc
     6961 attccgggac ccagtatttt cagatagttg cggaacctgc cttttaattt catgaatgaa
     7021 tggttgtcat tcccctccgc tgcgaaaggt aaattaaaaa tcgtaccgcc gcgtacggca
     7081 gcccaatgac cggtgatgca gtcaaaaagg ccaggtcctt acccgggaga ataagaggac
     7141 cggggagaaa ggtcttctgt tttaatacaa tcattaatga tcacagaaag cgcttcggat
     7201 tcgataagga aaccatcatg accgaaaaga gaggggatca cgaaatagga tgcccccggg
     7261 atatttgctg caagaaactc ctgttctttt tgcggaaaca gcagatcgga atgaatgcta
     7321 acgacatggg tctttgccct gattcttttt aatgcctgca ggatgcttcc gcgtccccgt
     7381 cccacatgat gcgaatccat actttgggaa agacggtaat aactgaacgc attgaagcgg
     7441 cgcgccagtt tctctccctg gtagacctgg taggattcac tgcggtaagg ccccagggaa
     7501 ttaatgtccg gatccccctg gtacaaatca taggtttccg gatgacggta ggaaagcaaa
     7561 gcaatgcccc gggccacttt catcccctgc acacccgcca ggggatggcg ttgaagccat
     7621 tccggatcaa gttcaatgca taatcgctgg gaagcattga aggcctttcc ccagggggaa
     7681 tgaatggcat tggtggcgat gggaacaatg tgttcaaaca gatcgggttc ttcgatggcc
     7741 cattccaaca gttgttgccc acccatggag ccgccaatgc caatttttat ttttttaatt
     7801 cccaaaaatt cacggagcgg ctggtaggcc cggatcatat cccgcgtggt aaaaaacggg
     7861 aagtcgtgat agtaaggctg gcccgtttcc ggattatttt ccagcggacc aatgctgcca
     7921 taacaactcc ccggcatgtt cacgcagacc agaaaatatt tttcgggatc gaataaa
//
LOCUS       DASYCU010000045         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3203403, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000045 DASYCU010000000
VERSION     DASYCU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3203403"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VGM24_00920"
     CDS             complement(<1..411)
                     /locus_tag="VGM24_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY4153948.1"
                     /translation="MDRIILASASPRRKQLLEWAGISFDIVPADADERIPSGLTWEEI
                     PVEIARRKAALVKKNTEPGRIILAADTLVIVDREVIGKPVDRADAVRMLSLLSGRRHQ
                     VITGVVITDARKEIAFAEITEVWFHDLDICGIEYY"
     gene            complement(399..1364)
                     /locus_tag="VGM24_00925"
     CDS             complement(399..1364)
                     /locus_tag="VGM24_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HEY4153949.1"
                     /translation="MKWNATIAFFRLIRWPNLLFIALTQCLFYYCILLPSFYNADPSR
                     ENILRPPEFVLLCISSVLIAAAGYIINDYFDLHIDRVNKPEKIVVEKIIKRRWAIIWH
                     WILSILGVGIGFYLSWKLRNIFIGPSNLACVLLLWFYSTTFKKKLLFGNILISFLTAW
                     VIGVLYLCEFRLHRFVNPEFHAVLSRVYKFAILYASFAFIISLVREVVKDMEDLEGDA
                     RYGRRTMPVVWGINASRVFAGTWLMLLMAALIGIEFYILPYRWWGVVIFSALFVIFPM
                     LWVLKKLYRADTPADFHRLSRMIKIIMMMGILSMIFLKIYTSWIG"
     gene            complement(1385..1906)
                     /locus_tag="VGM24_00930"
     CDS             complement(1385..1906)
                     /locus_tag="VGM24_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY4153950.1"
                     /translation="MNLLEFFKPVTSFIFDIDGVLTDGTIQVFESGEQVRQMSVKDGF
                     ALQLAVKKNYQVAVVSGANGLGAKNRLLKLGIRDVFLQVSDKKKQIEEYLQQHNLQWK
                     EVLYMGDDIPDMAALKMAGLPCAPSDAVPEVLQMVKYISTRPGGSGCVRDVIEKVLRL
                     QGNWDLEAGVSSR"
     gene            complement(2015..>2474)
                     /locus_tag="VGM24_00935"
     CDS             complement(2015..>2474)
                     /locus_tag="VGM24_00935"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY4153951.1"
                     /translation="YGVLYPLQSFRRELEDQPEFPLLIDANSPGSLEELKKMAAGLSK
                     TILPAGNEMRLKYHLAAVLAGNFSNHLYSLTESWCRREEIDFGLLQPLIRETAGRLLN
                     ELPENLQTGPAIRNDTETLEKHRELLQKYPELSQLYEVMTRSIQQFHHIQ"
BASE COUNT          663 a          625 c          590 g          596 t
ORIGIN      
        1 ataatattcg attccgcaaa tatccaggtc atgaaaccat acttccgtga tttcagcaaa
       61 agcaatttcc tttctggcat ctgtaataac aacaccggta attacctggt gccttctgcc
      121 ggaaagcaga cttagcatgc ggacggcgtc tgctctatcc accggctttc cgatcacttc
      181 ccggtctacg atcacgaggg tatcggccgc caggatgatc cttccgggct ccgtattctt
      241 ttttacgagg gcggctttcc ggcgggcaat ttccaccgga atttcttccc aggtgaggcc
      301 ggagggaatc cgctcatcgg catcagccgg aacaatatca aatgaaattc cggcccattc
      361 cagtaattgt tttcgcctgg gggaagccga agccaggatt atccgatcca tgaagtatat
      421 atttttagaa agatcatgga aagaatgccc atcatcatga tgattttgat cattcggctg
      481 aggcggtgaa aatccgccgg agtatcggcc cggtataatt ttttaaggac ccacagcatg
      541 ggaaaaataa caaataaggc ggagaagata accactcccc accaccggta gggcaatatg
      601 taaaattcga tcccgatcag cgctgccatc agcagcatca gccaggtgcc ggcaaagacc
      661 cgggatgcat tgattcccca aaccaccggc atggtcctcc ttccgtaccg cgcatctcct
      721 tccaggtctt ccatatcctt aaccacttcc cgcacaaggg aaatgatgaa tgcaaaagag
      781 gcatagagaa tggcgaattt atataccctg gaaagaacgg catgaaattc cgggttcaca
      841 aaccggtgga ggcggaactc acacagatat aagaccccga ttacccaggc tgtcagaaag
      901 gagatcagga tattgccaaa caataatttt tttttgaacg tggtactgta aaaccacaat
      961 aacaatacac aggccaggtt ggaagggccg ataaagatat tgcgcagctt ccaggagaga
     1021 taaaatccga tcccgacgcc cagaatcgat aagatccagt gccagatgat ggcccatctc
     1081 ctttttatga ttttttccac gactattttt tccggtttgt tcacgcggtc gatgtgcaga
     1141 tcgaaataat cgttgataat atatcctgcc gcagcaataa gcacggaaga aatacataaa
     1201 agcacgaatt cgggcggtct gagaatattc tccctcgacg gatctgcatt ataaaagctg
     1261 ggcaacagga tgcaatagta aaacaggcac tgggtaaggg cgatgaacaa aagattcggc
     1321 cagcggatca aacgaaaaaa agcaatcgtg gcattccatt tcatagtaca aagtttgcag
     1381 ccgtttagcg ggaacttacc cccgcttcca gatcccagtt cccctgcaac cggagaactt
     1441 tttcaattac atcccgcaca cagccgctgc cgcccggacg ggtggaaata tatttaacca
     1501 tctggagtac ctccggcacc gcatcggaag gcgcgcaagg caaaccggcc attttcagcg
     1561 cggccatatc cggaatatca tcccccatgt acaatacttc tttccattgc aggttgtgtt
     1621 gctgaagata ttcttcaatc tgctttttct tatctgaaac ctgcaggaag acatcccgga
     1681 tgccgagttt taacaatctg ttttttgccc ccagcccatt ggctccggaa acgaccgcca
     1741 cctggtaatt tttttttacg gcaagctgaa gggcaaagcc gtccttcaca ctcatctgcc
     1801 tgacctgctc gcccgattcg aatacctgga tggttccatc ggtcagtacg ccgtcgatgt
     1861 caaatataaa actggtaacc ggcttgaaga attcgaggag attcatagat tgaattggga
     1921 aaaaggtaaa ggtgaaaacg ctttgcctgc aaaagtattg gattaaatgc gaaacgcata
     1981 acgcggaagg ttccggcgcc ggagcgacaa tagtctactg aatatggtgg aattgctgaa
     2041 tgctccgggt catgacttca tagagttgag aaagctccgg atatttttgg agaagttcgc
     2101 ggtgcttttc taaggtttcc gtgtcgttgc ggatcgcggg tccggtttgc agattctccg
     2161 gcaattcatt cagcagacgg ccggccgttt cccggatcag gggctgcaac aggccaaaat
     2221 caatctcctc ccggcggcac caggattccg tgagggaata taaatggttg ctgaaattgc
     2281 cggccagcac ggcggccaga tgatatttta aacgcatttc atttcctgcc ggaagaatgg
     2341 ttttgctcag gccggcggcc atttttttta attcctccag gcttccggga ctgttggcat
     2401 cgatcaataa gggaaactcc ggctggtctt ccagttcccg ccggaaactc tgcaacgggt
     2461 agagcacgcc gtac
//
LOCUS       DASYCU010000046         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_883770, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000046 DASYCU010000000
VERSION     DASYCU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883770"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VGM24_00940"
     CDS             <1..747
                     /locus_tag="VGM24_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153952.1"
                     /translation="AVSLLATQKPLMILKEDKDSIYIRADTLFSGKIPDSLINRIDSV
                     QGTAIIKTAHNPNDSSLRFLQAYHHVRIFSDSLQAVADSLYYSGMDSIFRLYTNPVAW
                     AGGYQITGDTMFLYTRNKKADRLYVFENGLVVGKVVGNMYNQVKGNTINGYFRDGVID
                     YMRAKGSAESVYYVKDDSMALVSVNRVNKADVIDMIFKDKALNKVVLRNDVEGVMNPI
                     RKVNPEDMKLKNFKWLDALRPKTKFELFEN"
     gene            complement(815..2047)
                     /locus_tag="VGM24_00945"
     CDS             complement(815..2047)
                     /locus_tag="VGM24_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HEY4153953.1"
                     /translation="MALNLIWIIFFLIAFVTALIRLVFYGDTDIFKTIVDGVFDSANV
                     SVQIAIGLIGVMALFLGFMNVGEKAGAVRFLSRIIGPFFNRLFPEVPKNHPSMGHMVM
                     NFSANLLGLDNAATPFGLKAMMSLQELNPNKQTASNAQIMFMVLHASGLTIIPVSIIA
                     QRAILKSTDPTSIFIPCMIATFAATVVALTVVAIKQRINLFQPVILLWVCGISGLIGG
                     LVFILRTHSSYIDPFSKIFSNGLILLLFVVFIIGGMIKKLNVYEAFIDGAKEGFQVAV
                     TIIPYLVAMLVAISVLRSCGVFDYIVKGIGSFFVMLHVNTDFVPSLPTALMRPLSGSG
                     GRGMMIETMRIYGPDSFPGKLSCVFQGTADSTFYIAALYFGSVGIKNIRYAIPAALLA
                     DLAAIVTAILVSYLFFMH"
     gene            2159..>2540
                     /locus_tag="VGM24_00950"
     CDS             2159..>2540
                     /locus_tag="VGM24_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY4153954.1"
                     /translation="MALKAGIVGLPNVGKSTLFNAVSSSAKAQASNYRFCTIEPNLGL
                     VDVPDARLDRLAELVHPERIVPTQIEIVDIAGLVKGASKGEGLGNKFLANIREVDAII
                     HVIRCFEDENILREEGAINPVSDKE"
BASE COUNT          722 a          621 c          645 g          552 t
ORIGIN      
        1 gcggtgagcc tgctggccac ccagaaaccg ctgatgatcc tgaaagaaga caaggattcc
       61 atctatatcc gggccgatac gctgttttcc ggaaagatcc cggattccct gatcaaccgg
      121 atcgattcag tgcagggaac agccatcatc aaaacggcac ataatccaaa cgacagcagc
      181 ctccgctttt tgcaggctta ccatcacgtg cgcatcttct cggactccct gcaggcggtc
      241 gccgatagtt tatattattc cggcatggat tcgattttcc gtttgtatac caacccggtg
      301 gcctgggcag gcggttacca gataacggga gacaccatgt tcctttatac ccggaataaa
      361 aaagcggacc gcctctatgt atttgaaaac ggactggtcg tcgggaaagt ggttggaaat
      421 atgtataacc aggtaaaggg aaataccatc aatggttatt tcagggatgg cgtgatcgat
      481 tatatgcgcg ccaagggaag cgcggagagc gtttactatg taaaggacga cagtatggcc
      541 ctggtgagcg taaacagggt gaataaggcc gatgtgatcg atatgatctt caaagacaaa
      601 gcactgaaca aggtcgtcct gcgcaacgat gtggaagggg tcatgaatcc gatccgcaaa
      661 gtgaacccgg aggatatgaa actcaaaaac tttaaatggc tggacgcgct gaggcccaag
      721 acgaagtttg aattatttga aaactgatcc ttttcttttt cagtcgtacc cgttttccgg
      781 tgccctgggt taccggcaaa tccgggatcc gcgcctaatg cataaaaaat aaataggaaa
      841 ccaggattgc cgtgacgatc gccgccagat ccgccagcaa cgcggcagga atggcataac
      901 ggatattctt gatgccgacc gaaccgaagt acagggcagc gatataaaaa gtggaatccg
      961 cggtaccctg gaaaacacag gaaagtttgc cgggaaacga atccggtccg tatatccgca
     1021 tggtctcgat catcatgccc ctgccgccgc taccgctgag cggccgcatg agcgccgtcg
     1081 gcagggatgg gacaaaatcc gtatttacat gcagcatcac aaaaaaagaa ccgatgccct
     1141 tcacgatata atcgaagacc ccgcagcttc tcagtacgct gatggccacg agcatcgcca
     1201 ccaggtaggg gataatggta accgccacct ggaatccttc ctttgcgcca tcgataaaag
     1261 cctcgtacac gttcagcttc ttaatcattc cgccgataat aaaaacgacg aataataaca
     1321 gaatgagccc gttgctgaag atttttgaaa aaggatcaat ataactgctg tgcgtgcgca
     1381 atataaagac cagccccccg atcagccccg aaattccgca gacccataac aggatcacgg
     1441 gctggaataa attgatccgc tgtttaatgg ccacgaccgt cagcgcaacc acggtggcgg
     1501 caaaagtggc gatcatgcag ggaatgaata tactcgtcgg gtcggttgat ttcagaatgg
     1561 ccctttgagc gataatgctg acggggatga tggtcagacc cgatgcatga agcaccatga
     1621 acatgatctg cgcgttcgag gcggtttgtt tattcgggtt gagctcctgg aggctcatca
     1681 tggcttttaa cccgaagggc gttgccgcat tgtccagccc cagcaggttg gcggaaaaat
     1741 tcatgaccat atgccccatc gaagggtgat ttttcggcac ttccggaaac agccggttaa
     1801 aaaatggccc gatgatccgg gaaaggaacc ggacggcgcc ggctttttcg cccacattca
     1861 taaatcccag gaaaagcgcc atcacgccga tgagcccgat ggcaatctgt acgctcacat
     1921 tcgcagaatc aaaaacgccg tccacgatgg tcttgaaaat atccgtatcg ccgtaaaaaa
     1981 caagccggat cagggcggta acaaaagcaa tcagaaaaaa aatgatccag atgagattca
     2041 gcgccatatc cttccattaa ttatgggttg aatataagta ataaaataac gatctcttaa
     2101 atacttctat ctttgcgggc tcaaaaacat ttcgtaccgg aattaaaata aacaagtcat
     2161 ggcattaaag gcaggcattg tcggtttacc caatgtaggc aaatccacct tgttcaatgc
     2221 agtcagcagc agtgcgaagg cccaggccag taattaccgc ttttgtacga ttgagccgaa
     2281 cctgggactg gtggatgttc ccgacgcccg tttggatagg ctggcggaac tggtccatcc
     2341 cgagcggatt gtacccaccc agatagaaat tgtggatatt gccgggctcg tcaaaggagc
     2401 cagcaagggc gaaggtctcg gaaataaatt cctggccaat atccgtgaag tagacgccat
     2461 catccatgtg atccgctgtt ttgaagatga aaatatattg cgggaagaag gtgccatcaa
     2521 tccggtaagc gataaggaaa
//
LOCUS       DASYCU010000047         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2622511, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000047 DASYCU010000000
VERSION     DASYCU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622511"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..888
                     /locus_tag="VGM24_00955"
     CDS             412..888
                     /locus_tag="VGM24_00955"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4153955.1"
                     /translation="MQKISPFLWFDDNAEEAMNFYLSVFKNSGVIRKAHNPPGGPGPE
                     AALLVASIELEGQEITLMNGGPGHPLTDAISLTINCSSQEEVDRYWNKLLEGGGKEVA
                     CGWLKDRFGLYWQVTPTMLPELLSDPDRAKAARVMQAMMKMIKLDIPALKAAADAK"
     gene            complement(895..>1551)
                     /locus_tag="VGM24_00960"
     CDS             complement(895..>1551)
                     /locus_tag="VGM24_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEY4153956.1"
                     /translation="KETKRMIGLVRNFTGFREYPKYSMISRYWVYKQALLKEAGQLLR
                     AGVIQEKEDIYFLRFEELREVLSINKLDYRIIDKRKDEFRSYEKLTPPRVMTSDGEII
                     SGRYNRENLPAGAIAGLPVSSGVVEGRARVILKMEDADLEEGDILVTVFTDPSWTPLF
                     VSIKGLVTEVGGLMTHGAVIAREYGLPAVVGIENATRLIPDGQRIRVNGTEGYVVILE
                     "
BASE COUNT          379 a          402 c          368 g          402 t
ORIGIN      
        1 ttcagcaaaa aaacctaggg tcacagcgat aaatatcatt aacccttcca gcaaatactc
       61 tttaaggttt ttatttacaa gatggggatt atgatgaact tccatttctt cagtttgttg
      121 aggaggagtt attttatcat cgcttgggtt cgcgttttca gtcatttggg gtctgcatta
      181 aatgctcaaa gaattgcggt atttaaatgg cctaatatac agtagattta tccccggatg
      241 cgcaacgact ttccggaaag ataagaaagc ttatttaact tttttttgcc ggacaaaaaa
      301 aaccggcttc cctatcggtc acccgattgc tttcacttat taattggaag aatcggcaca
      361 aactggcaaa cgatttgata tgcttaagag caagtcctta aaatttattt tatgcaaaag
      421 atcagtcctt ttctctggtt cgacgacaat gcggaagaag cgatgaactt ctatctatcc
      481 gttttcaaga attccggggt gattcgaaag gcccacaacc cgcccggcgg accgggtccc
      541 gaagctgccc tgctggtggc gtccatcgaa ctggaggggc aggaaattac cctgatgaat
      601 gggggaccgg gccatcccct gacggatgcg atctcgctta cgatcaactg cagctcccag
      661 gaagaggtcg atcgctactg gaacaagctc ctggagggtg gcggcaagga ggtcgcctgc
      721 ggctggctga aagaccggtt cggtctttac tggcaggtaa cgcccaccat gttgccggag
      781 ctgctcagcg atccggaccg ggcaaaagcg gcccgcgtca tgcaggccat gatgaaaatg
      841 atcaagctgg acatcccggc cctgaaggct gcggcggatg caaagtaacc gaggctattc
      901 cagtattacc acatatccct ccgttccgtt cacccggatc ctttgtccgt ccgggatgag
      961 ccgggtggca ttttcaattc cgaccacagc gggcaagcca tattcacggg cgatgaccgc
     1021 tccgtgggtc atcagtccgc ccacttcggt aaccaggccc tttatggata caaacagggg
     1081 tgtccaactg ggatcggtaa acacagtaac caggatatcc ccttcttcca gatcggcatc
     1141 ttccattttt aatatgaccc tcgcccttcc ctccacgacg ccggaagaaa ccggcagacc
     1201 ggcaattgct cccgccggga gattctcccg gttataccta cccgaaatga tttccccatc
     1261 agatgtcata acccgcggag gagtcagttt ttcataggat ctgaattcat cctttcgttt
     1321 gtctatgatc cggtaatcca gtttatttat gctcagcact tcccgaagtt cttcaaaacg
     1381 gagaaagtaa atatcttcct tttcctggat gaccccggct cgcaaaagtt gcccggcttc
     1441 tttcagcaat gcctgtttat aaacccagta gcggctgatc atgctgtatt tcgggtattc
     1501 gcgaaagccg gtaaaattcc ggaccaggcc gatcatccgt tttgtttctt t
//
LOCUS       DASYCU010000048         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_884025, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000048 DASYCU010000000
VERSION     DASYCU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884025"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGM24_00965"
     CDS             <1..891
                     /locus_tag="VGM24_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153957.1"
                     /translation="YEEFAEMCVAYVKIIKQQAGIDVYALSIQNEPRFSQSYESCVYD
                     GNALRDLLKTVGSRFKSEGITTKLFLPEDIGYLEGVEGMIKPSLDDAEARQYADIVAV
                     HGYALDGVTAASPDAQTWKTMYSWGAAYGKPLWMTETSGYPDNFDGAMALAKAMLTAI
                     KFGNVSAWLFWQLSQSSIDEFSLMSTAGAKSKRYYASKNFYKFIRPGAVRISAEAPED
                     SKIYPVAFRSAAGDSTILVLINDNPDQGKLVKLSSAGLPSSFELYTTTETDDCADKGA
                     VNTAEPFMLPPNSIVTLAGK"
     gene            complement(903..>2279)
                     /locus_tag="VGM24_00970"
     CDS             complement(903..>2279)
                     /locus_tag="VGM24_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY4153958.1"
                     /translation="KKAWRQNVLPPMAKMLGLDYLYETPVSTKKKASLSGAEWEAIVR
                     YYLGKAPDSLPGQGRAPVRAFSNLFQAKRVLVPEGKFPSTAYVRIDPGNHLIYQGNAS
                     DSSLNTYDDRLNPVAVRNIHGVLIDMNFSTSLQEAGERTGILTNIGMMNPNDLKTGSA
                     DSFRITPKGTLSYLARVLDSMPRPVQTISCDLNQDGIRDYLVCGFGNMAGALYWVTKE
                     KKGNTEEKIIRALPGAIKAYIDDYNGDGRPDMMVLMAQAQEGIFLFLNKGNGSFETRE
                     VLRFPPIYGSSYFELDDFNGDGFKDILYTCGDNGDFTKHVLKNYHGIYIFLNDGRNHF
                     TQKYFFPIHGCYKAMARDFDQDGDLDIAAISFFPDTANQPQESFVYLEQTKSLEFSPY
                     TIKEFNEGNWLTMDVGDVDGDGDEDIVLGSLIPPYPGREQKWLKEGAYKTSLLLLENK
                     LKRRPGPK"
BASE COUNT          544 a          594 c          559 g          582 t
ORIGIN      
        1 tatgaagaat ttgccgaaat gtgcgtggcc tatgtaaaaa ttatcaaaca gcaggccggc
       61 atagatgtat atgcattaag tatccagaac gaaccgcggt tttcccaatc ctacgaatcc
      121 tgtgtgtacg acggaaacgc tttgagagat ctgctaaaga cggtgggaag ccggtttaaa
      181 agcgagggca taacaacaaa actgttcctg ccggaagata taggctatct ggagggggtt
      241 gagggaatga ttaaaccttc gctcgatgat gcggaggcca ggcagtatgc cgatatcgtt
      301 gcggtgcatg gttatgcgct ggacggagtg accgccgcct cgccggatgc acagacctgg
      361 aaaaccatgt acagctgggg cgccgcctac ggcaagccct tgtggatgac ggagacctcc
      421 gggtatcccg ataattttga cggggccatg gccctggcca aagccatgct aaccgctatt
      481 aaattcggca atgtctcggc ctggcttttc tggcaactga gccaatcctc catagacgaa
      541 ttttccctga tgagtacggc aggcgcaaaa agcaaaaggt attatgcgtc caaaaacttt
      601 tacaaattta tccgtccggg agccgtccgc atttccgccg aagcgccgga agattcaaaa
      661 atttatccgg ttgcattccg gagcgccgcc ggcgattcaa ccatcctggt attgatcaat
      721 gataacccgg accaggggaa gctggttaaa ttatcttccg cggggcttcc ttcttcattt
      781 gaactgtata ctactaccga gaccgatgat tgcgctgaca aaggcgccgt taatacggcc
      841 gaacccttca tgcttcctcc gaacagcata gtcaccctgg ccgggaaata agaccagcat
      901 ctttatttcg gtccgggcct tctctttaat ttattttcca ggaggagcag cgaggttttg
      961 taagcacctt ctttcagcca tttctgttcc cggcccggat agggcgggat caggctgccc
     1021 agcacgatat cctcatcgcc gtcgccgtca acatccccga catccatggt aagccagttc
     1081 ccttcattaa attcttttat ggtatacggg ctgaactcca gactttttgt ttgttccagg
     1141 tacacaaagg attcctgcgg ctggttggcg gtatcgggaa agaaagatat cgcggcaatg
     1201 tcgaggtccc cgtcctgatc aaaatccctg gccatcgctt tgtaacatcc gtggatgggg
     1261 aagaaatact tctgggtaaa atgattcctg ccatcattca gaaagatgta aatgccgtga
     1321 taatttttca ggacatgttt tgtaaaatcg ccgttatctc cgcaggtata taatatatcc
     1381 ttaaacccgt ctccgttaaa atcatccagt tcaaaataac tcgatccgta tatcggaggg
     1441 aaacgcaaaa cttcccgggt ttcaaaacta ccgttccctt tattcaagaa taaaaatata
     1501 ccttcctgcg cctgcgccat gagcaccatc atatccgggc ggccgtcccc gttataatca
     1561 tcgatatagg ccttgatcgc tccgggcaat gcccgtatta tcttctcttc cgtgttccct
     1621 ttcttttctt tcgtaaccca gtataaggcg cctgccatgt ttccgaaacc gcagaccagg
     1681 taatcccgta ttccgtcctg gttgaggtcg caggaaatgg tctgaacggg cctgggcatg
     1741 ctgtcgagca cccgggccag ataggataat gttcccttcg gagtgatccg gaaactgtct
     1801 gcagatcctg ttttcagatc gttcggattc atcatcccga tattcgtcaa tatcccggtc
     1861 ctttctccgg cttcctgcag ggatgtgctg aaattcatat cgatcaggac gccgtgaata
     1921 ttgcggacag ccacggggtt tagccgatca tcataggtgt tcagggaaga gtccgatgca
     1981 tttccctggt agatcaggtg attgcccgga tcaatcctga catacgcggt ggaagggaat
     2041 ttgccttcgg gaacaagcac ccgtttagcc tgaaaaagat tgctgaacgc ccggaccggc
     2101 gccctgccct gccccggcag ggagtcgggc gctttgccaa gataataccg cacgatcgct
     2161 tcccactctg cgccggaaag ggatgccttt tttttggtgc ttaccggagt ctcatacaga
     2221 taatccaatc ccagcatttt tgccatgggc ggcagaacat tttgtctcca cgctttttt
//
LOCUS       DASYCU010000049         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_884324, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000049 DASYCU010000000
VERSION     DASYCU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884324"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..800)
                     /locus_tag="VGM24_00975"
     CDS             complement(24..800)
                     /locus_tag="VGM24_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY4153959.1"
                     /translation="MKAIWTGAIGFGLVNIPVRIFSAVQDSELDLDMLDKKDHARIRF
                     KRVNEVSGREVDWNNIVKAYDFNGKYVVVTDEDFAKASPEKSKIIEIMEFVKESEIDS
                     IFYETPYFLEPQKAGEKAYALLREALKKSGKVGVGNYVMRSKEALCILKPMDDVIILN
                     RIRFAQEIRNVNEINVPGKTSIKPGEMQMAQALISQLSGKFDISKYKDSYSDELLKLI
                     RAKAKGKKTEMPQMRVVHNKSKDLMSQLKASLSTKKKKVS"
     gene            complement(848..1192)
                     /locus_tag="VGM24_00980"
     CDS             complement(848..1192)
                     /locus_tag="VGM24_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4153960.1"
                     /translation="MPEKRVLIIDDEADFCLLIKNYFVKKNYEVHISHTLKEGMESLE
                     NFKPDILFLDNNLPDGLGWEKTEYITKNYPNIRINLISAYQYNPTISEKFSSVKIWEK
                     PISMSELNQYLN"
     gene            1443..1589
                     /locus_tag="VGM24_00985"
     CDS             1443..1589
                     /locus_tag="VGM24_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEY4153961.1"
                     /translation="MRTLLYVIAVILIIGWILGFFVYSAGGLIHILLVIAIIALILGV
                     IRRA"
     gene            1695..2252
                     /locus_tag="VGM24_00990"
     CDS             1695..2252
                     /locus_tag="VGM24_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153962.1"
                     /translation="MNIIETIQKNLGFEPIQKIDPNSQEVANKEAIHGNTALAQAAIP
                     SILLGLFNRLEKERDASWLAGDQPTGRLLEKIFGQSNDKVIEIVSVYCLTTDQNVKQE
                     MEHIASESVRVVRDHVADLTSEDSIAAFVSQHKHEVLLYLPASLQLGSILGNDNLDDR
                     TNKMEGPVSGLMHRIEKQFNSSGNN"
     gene            complement(2230..2676)
                     /locus_tag="VGM24_00995"
     CDS             complement(2230..2676)
                     /locus_tag="VGM24_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153963.1"
                     /translation="MKSSIETGAELNEAIAALEKKAVFQKEELKEHFAGVMENMKPLN
                     LIKNGLQSTFSGENKQDLLKTAIGIGSGMLGRKLLIGRAGGSILRKIFGAALEFGVVG
                     LVAKNAEKIKLKGSEFIENLFHKKKSSRSVLLPDPDQPGSNYSRKN"
     gene            complement(2683..3024)
                     /locus_tag="VGM24_01000"
     CDS             complement(2683..3024)
                     /locus_tag="VGM24_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153964.1"
                     /translation="MAERDISIKSLLEKSQDYLETKIEIARLKTIDKSSDLVSSVIVL
                     ISVILMALLFFLFISIGVALYLGTLVGSAHAGFFIVGGAYGIVLLLLYAFREKWVKAP
                     VANLIIKKLLK"
     gene            complement(3055..3258)
                     /locus_tag="VGM24_01005"
     CDS             complement(3055..3258)
                     /locus_tag="VGM24_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY4153965.1"
                     /translation="MTSKKLIAGILTGLAAGAMISILMASKKNRTFSKKLIRKGNNLA
                     EDLKGQFNDFVDRLEDKFQGILK"
     gene            3477..4259
                     /locus_tag="VGM24_01010"
     CDS             3477..4259
                     /locus_tag="VGM24_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4153966.1"
                     /translation="MATAEIRLPAWVPFKKAVLHAGEFSLFFFRFLRESVKLPFEGRE
                     FLFQCFKVGNKSLPLVGVTSFIMGLVLTLQTRPSLATFGAVSLLPGMVSVSVIKEIGP
                     VITAVICAGKISSRMGAELGAMKVTEQIDAMEVSAINPFRYLVVTRVLATTCMVPLLV
                     IFADCIALFSSFLAYNIHGEISITRFFDLALSKLDWIDIFPSLIKSVLFGTAIGIIGC
                     YKGFRVDGGTASVGTAANSAVVTASLAIFFIDLIAVQITDIL"
     gene            4262..4999
                     /locus_tag="VGM24_01015"
     CDS             4262..4999
                     /locus_tag="VGM24_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4153967.1"
                     /translation="METTEPIVRISSLRKTFNQVEVLKGIDLSIDKAENLVVLGKSGS
                     GKSTLIKCMIGLSEPDEGSIFIMGQNMAEVNYEELNKLRVKIGFLFQSAALYDSMTVR
                     ENLAFPLRHHKKHLGKEKINELIREVLENVGLPDAGDKMPAELSGGMSKRVGLARTLI
                     LKPSIMLYDEPTTGLDTATAKEISQLILDLQRKYQISSLIITHDISCAKMTADRIVML
                     KEGAIVAEGTYEELMASEDEWVKSFFG"
     gene            5019..>5430
                     /locus_tag="VGM24_01020"
     CDS             5019..>5430
                     /locus_tag="VGM24_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEY4153968.1"
                     /translation="MKSNAGLKWKLGLFIISGLVLLVLGLFYIGKQKNLFDSHFRLNA
                     VFSNVSGLKTGNNVRLGGINIGTVEGIELITDTSVMVRMSVKEKLQKFIHGNAIASIG
                     SEGLMGDRVVFITPGAAGGETVKDNAVLGSRAPVE"
BASE COUNT         1386 a         1279 c         1246 g         1519 t
ORIGIN      
        1 cccgaaacag caaaggttta aattcaggag acttttttct ttttggtact cagactggcc
       61 ttgagctggc tcatcaggtc ttttgatttg ttgtgcacca cccgcatctg tggcatctcg
      121 gtttttttgc cctttgcttt ggccctgatg agtttcagca gttcatccga ataactgtcc
      181 ttatatttgg aaatatcaaa ttttccgctg agctggctga tcagcgcctg ggccatttgc
      241 atttcgcccg gtttgatgct ggttttgccc ggcacattga tttcgttcac attccggatc
      301 tcctgggcaa accggattct gttcagtata atcacatcat ccatcggttt taaaatacaa
      361 agcgcttcct tgcttctcat cacgtaattc ccgacaccca cttttccgct tttctttaat
      421 gcttcccgaa gcagggcgta cgctttttcg cctgccttct ggggttcaag aaaataaggt
      481 gtttcgtaaa aaatggaatc aatttccgat tcttttacaa attccatgat ttcgatgatt
      541 tttgattttt ccggactggc ttttgcaaaa tcctcatccg taaccacaac gtatttcccg
      601 ttaaaatcat aggctttaac aatattattc caatcgactt cgcggccgga tacttcattt
      661 actcttttaa aacggatccg cgcgtggtcc tttttatcca gcatgtccag atccagctcg
      721 ctgtcctgca ccgcactgaa tatccgaacc ggaatgttta cgagtccaaa cccgatggct
      781 cccgtccaaa tggctttcat acaaaagatt tatactgtaa ggaactaaaa caatgccata
      841 tccgtgatca attcaggtat tgattcaatt ccgacatgct gatgggcttt tcccagattt
      901 ttacggatga gaatttttcg gaaatggtgg ggttgtactg ataagcgctg atcaggttga
      961 tgcggatatt ggggtagttt tttgtgatgt attctgtttt ctcccagcca agaccatccg
     1021 gcaggttgtt gtccaggaaa agaatgtcgg gtttaaaatt ctcgagggac tccatgcctt
     1081 ctttcaacgt gtgtgaaata tgtacttcgt agttcttctt gacaaaataa tttttgatta
     1141 aaagacagaa atctgcttca tcatcaatta tcagtactcg tttttccggc atcgtgaata
     1201 aggttcatgg gttgtttaaa agtcaaggga gtcagaccaa aaacctgcaa atcgttttca
     1261 aatataagga ctgtttataa aaaacgcctt gatcatttta cctttttttt gtagaaaatg
     1321 atctacaaac agcgggcaaa atctgcaaat gcacaaataa atgaatagga tccggtaaaa
     1381 gccatgctgg catgaaatta ttacctgttg tcaaacgatt agttaaccct aaaattaaat
     1441 ttatgcggac tctattgtat gtcattgccg ttattctgat cattggatgg atactgggat
     1501 tttttgttta cagtgccggc ggactcattc atatcctgtt ggtcattgcc attattgccc
     1561 tgattctcgg cgttatccgg agagcctgac gccattatct gtcaaagtgg gaagccattc
     1621 aatgctcgtt cacctgagaa tgggcatttt catttactgg catgtttttt agccaataac
     1681 cataaaagac gatcatgaat attattgaaa ccattcaaaa aaacctgggc ttcgagccca
     1741 ttcaaaaaat agatcccaat tcccaggaag tggcaaataa agaggccatt catggcaaca
     1801 cggcccttgc ccaggccgcc atcccttcca tattgctcgg cctgttcaac cggctggaaa
     1861 aggaacggga cgcctcctgg ctagccggag atcagcccac cggccggttg ctggaaaaaa
     1921 tttttggcca gtccaatgac aaagtgattg agatcgtatc ggtctattgc ctgacgacag
     1981 atcagaacgt aaagcaggaa atggagcata ttgcatcgga atccgtccgg gtggtccggg
     2041 atcacgtcgc agacctcacc agcgaggaca gcattgccgc attcgtttcc cagcataagc
     2101 atgaggtgct cctttatctg ccggcttccc tgcaactggg gtccatactc ggaaacgata
     2161 atctggacga ccggaccaat aaaatggaag gcccggtatc gggcctgatg caccggatag
     2221 aaaaacaatt taattcttcc gggaataatt agaaccgggt tggtccggat caggtaaaag
     2281 aacggaacgg gaagactttt ttttatgaaa gaggttctcg atgaattccg aacccttgag
     2341 ttttattttt tccgcattct tcgccaccag gcctaccact ccgaattcca gtgccgcacc
     2401 gaatattttt cgcagtatac taccgccggc tcttccgatc agcaattttc ttcccagcat
     2461 ccccgatccg atgccgatgg ccgtcttgag caaatcctgc ttattttccc cggaaaaagt
     2521 ggattgcaac ccgttcttga tcaggttaag cggtttcata ttttccatca cgccggcaaa
     2581 atgctccttt aattcctcct tctgaaagac cgcttttttc tccagcgctg caatcgcctc
     2641 attcaattcc gctccggttt ctatagaact cttcatggtt gattatttca gcagtttttt
     2701 aatgatcaga ttggcgaccg gtgcttttac ccatttctcc cggaacgcat agaggagcaa
     2761 aagcacgatt ccataagcac ctcccacgat aaaaaacccg gcatgcgcgg accccaccag
     2821 ggtgcccagg taaagggcca cgccgatact gataaaaaga aaaaacagga gggccattaa
     2881 gatcacggat atcaggacaa taacagaaga taccagatcg gacgatttat caatcgtttt
     2941 cagcctggct atttcaattt tcgtttctaa ataatcctgt gatttctcca gcagcgactt
     3001 gatggatata tctctttctg ccataatgat agttaatgct tacgccaaag ttagtcactt
     3061 taaaatcccc tggaatttat cttccagccg gtccacaaaa tcattgaact ggcctttcag
     3121 gtcttctgcc aggttattac ccttcctgat gagttttttg ctgaaggtgc gattcttttt
     3181 agacgccatc aggatgctga tcattgcccc cgcggccagc cccgttaaaa taccagctat
     3241 taatttcttg cttgtcatat gtttctgttt tatgaaagta tagcataaaa atcaggccat
     3301 gccggtccga tttttattta taaatcccgg attctgcctt caaagggacc acgcggatgc
     3361 tgggtacata tttccacgaa tcccgctttg aaatcgcgca gttcaatcct gatgccccga
     3421 taatgtcccg gcacgatctt cttactgaca ggctaaccac tacatccaat cagtttatgg
     3481 ctacagcaga aatccgttta ccggcatggg tcccttttaa aaaggccgtc ctgcatgcgg
     3541 gagaattcag tttatttttt ttccggtttc tccgggaatc cgttaaactt cccttcgaag
     3601 gaagggagtt cctgttccaa tgttttaagg tggggaataa atcattgccc ctggtgggcg
     3661 taacctcctt tattatgggc ctggtgctga ccctccagac gcgtccttcg ctggccacgt
     3721 tcggggcagt ttcccttttg cccggaatgg tcagtgtttc cgtgatcaaa gaaatcgggc
     3781 cggtgatcac ggcggttatt tgtgccggta agatcagttc ccgcatgggc gcggagctgg
     3841 gcgccatgaa ggtcacggag cagattgatg cgatggaggt atcggcgatc aacccttttc
     3901 gctacctggt cgtgacgcgg gtattggcca ccacctgtat ggttccgctc ctggtcattt
     3961 ttgccgactg cattgccctc ttctcctcct tcctagccta taatatccac ggtgaaatca
     4021 gcattacccg gttctttgac ctggcgcttt ccaaactgga ctggattgac atatttcctt
     4081 cgcttatcaa atccgttctc ttcggaacgg caatcggaat aatcggctgt tacaagggat
     4141 tccgggtgga tggaggtacg gcgagtgtgg gaacagcggc caattccgcg gtggtaacgg
     4201 cctcgctggc catatttttc attgacctga ttgcggtaca gatcacggat attttgtgaa
     4261 aatggaaaca acagaaccga tagtacgaat cagttccctc cgcaaaacgt tcaaccaggt
     4321 ggaagtgctc aagggaattg acctttccat agacaaggcg gaaaacctgg tggtgcttgg
     4381 aaaatccgga tctggaaaat ccacgctgat caaatgcatg atcggcctga gcgaaccgga
     4441 tgaagggtcc attttcatta tgggtcaaaa tatggcagaa gtcaattacg aagagctgaa
     4501 taagctacgg gttaaaattg gttttctctt tcagagtgcc gccctttacg attcgatgac
     4561 ggtcagggag aacctggctt ttcccctccg gcatcataaa aagcatctgg gaaaagaaaa
     4621 gatcaacgag cttatccggg aggtgctgga gaatgtgggg cttccggatg ccggggataa
     4681 aatgccggct gagctctccg gcggaatgag taaacgcgtg ggactggccc gaacgctgat
     4741 cctgaagcca tccattatgc tttatgatga accaaccact ggtctggaca cggcaacagc
     4801 aaaagagatc agtcagctga tcctggacct gcagcgtaag tatcagattt cttcactgat
     4861 cattacgcac gatatcagct gcgcaaaaat gaccgctgac cggatcgtga tgctgaagga
     4921 gggagccatc gtggcagaag gcacgtatga ggaattgatg gccagtgaag atgaatgggt
     4981 aaaatcgttt ttcggttaaa cggataaata gcacgcatat gaaatcaaat gccggtctca
     5041 aatggaaatt aggcctcttt atcatcagcg gcctggttct gctggttctt gggttgttct
     5101 atatcggtaa acaaaaaaac ctgttcgact cccatttccg gctgaatgct gttttcagca
     5161 acgtaagcgg attaaaaacc gggaataatg taaggctggg cgggatcaat atcgggaccg
     5221 ttgaagggat agaactgatc accgatacct cggtgatggt gcgcatgtcg gtaaaagaaa
     5281 aactgcaaaa gtttattcac gggaatgcta ttgccagcat tggttctgaa ggattgatgg
     5341 gcgaccgggt agttttcatt acaccgggag ccgccggcgg ggagaccgta aaagacaacg
     5401 cagtactggg ttcccgggca ccggtagaaa
//
LOCUS       DASYCU010000050         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2477970, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000050 DASYCU010000000
VERSION     DASYCU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477970"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VGM24_01025"
     CDS             <1..262
                     /locus_tag="VGM24_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153969.1"
                     /translation="QNKLKALVAFGEVKTAEIQSMLSQEISRKIKDLSQMEKESRKLA
                     AQKQKELQPLINKVRENIKQQQNEIRMLKKQLKATAEGIIFI"
     gene            314..832
                     /locus_tag="VGM24_01030"
     CDS             314..832
                     /locus_tag="VGM24_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153970.1"
                     /translation="MNLDYRNLLPSEFHPGSRVWIYQSSRLFTIPEALQAEDLLSEFV
                     EGWLSHGSPVKGFATIFFGQFIVLMADETEVSLGGCSTDTSVRLIKSIENRFKLHLFD
                     RQSLAFIVNDKIQLLPYTQLAYAIENGFINPETLYFNNTVQCKSDLEENWIIPLKNSW
                     LKNRVVFKQLTG"
     gene            889..961
                     /locus_tag="VGM24_01035"
     tRNA            889..961
                     /locus_tag="VGM24_01035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:922..924,aa:Gly,seq:ccc)
     gene            1066..2487
                     /gene="miaB"
                     /locus_tag="VGM24_01040"
     CDS             1066..2487
                     /gene="miaB"
                     /locus_tag="VGM24_01040"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY4153971.1"
                     /translation="MEVLTTRMNKEKMQGEVYAPDKAVPPGSTKKFYLESYGCAMNFA
                     DSEIVASILKDSGFTASPTYLDADLVLVNTCSIREKAEQTIRNRLHLFRQAKRSSPGM
                     LIGILGCMAERLKAKLLEEEKLVDLVVGPDGYRTLPVLIEAAEGGQKSVNVLLSREET
                     YADISPVRLNSNGVNAFVSIMRGCNNMCSFCVVPFTRGRERSRDARSILNECQDLFDQ
                     GYREITLLGQNVDSYHWHSPDAEAFSFAMLLEQVARISPLMRVRFSTSHPKDITDEVL
                     VVMSRHENICNYIHLPVQSGSNRVLQLMNRTYTREWYLAKVRRIRELIPDCGISSDII
                     AGFCTETEDDQQETLSLMEEARFDMSYMYFYSERPGTLAARRYTDDVPEAVKKRRLAE
                     IVKWQNKISQLSNRRDIGTISKVLIEGDSRKSDQDWMGRNSQNKVVVFPKQGHILRKG
                     DYAILLIVDCTGATLIGALISGS"
     gene            2538..>3115
                     /locus_tag="VGM24_01045"
     CDS             2538..>3115
                     /locus_tag="VGM24_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HEY4153972.1"
                     /translation="MELQSIKNRFGIIGNSAELNYALTVATQVANTDLTVLIFGESGV
                     GKEAFSHIIHSLSARKHNPFIAVNCGAIPEGTIDSELFGHEKGSFTGAVDSRKGYFET
                     VNGGTIFLDEIGELPLGTQARLLRVLEAGEFIRVGSSKVQKTDVRVIAATNKDLLQLT
                     ERGKFREDLYYRLSTVPIRVPSLRDRKEDIPL"
BASE COUNT          827 a          738 c          813 g          737 t
ORIGIN      
        1 tcagaataaa ttaaaagccc tcgtggcttt cggggaggtg aaaacggctg aaatacaatc
       61 catgctcagt caggagatca gccgcaaaat caaagacctg tcgcagatgg aaaaggaaag
      121 ccggaaactg gccgcacaaa aacaaaagga actccagcct ttgataaaca aggtccgcga
      181 aaatataaag cagcagcaga acgagatcag gatgctgaaa aaacaattaa aagcaaccgc
      241 tgagggaatt atctttatct gacgccctgc gcgatccgct tttcatttcg cgtaaaagaa
      301 ttaattttac tctatgaacc tcgattaccg gaatcttttg cccagtgaat ttcaccccgg
      361 ttcacgagtg tggatctatc agtccagccg cctgtttacg atccctgaag ccctgcaggc
      421 agaggatctg ctgagtgaat tcgtcgaagg ctggttgtct cacgggtcgc cggtaaaagg
      481 ctttgccacc atattcttcg gacaattcat tgtattgatg gctgacgaaa ctgaagtcag
      541 cctgggcgga tgcagcaccg atacctctgt acgactcata aaatccatag aaaaccggtt
      601 taagctgcat ttattcgacc ggcagtccct cgcctttatc gtgaatgaca aaattcagct
      661 cctgccttat acccagttgg cttatgccat tgaaaacggg ttcatcaacc cggagaccct
      721 gtatttcaac aatacggtcc aatgcaagtc agacctggag gaaaactgga tcattccgct
      781 taagaacagc tggttgaaga accgggtcgt ttttaagcaa ttgaccggtt aatccgctgt
      841 atttattctt aacttagccg ccgccggtaa aaataatctg atcgatttgc ggaagtagct
      901 cacctggtag agcgacagct tcccaagctg taggtagcgg gttcgagtcc cgtcttccgc
      961 tcaaaattct tctctcctgc cgggttcccg gcatttgctc ctgtcatcct gtcatttctc
     1021 ccatctttta attatttttg cagtcccgta atacgggagc cgcccatgga agtactgacc
     1081 acaagaatga acaaggaaaa aatgcagggg gaagtatatg ccccggacaa agcggtcccg
     1141 cctggatcaa ccaaaaaatt ttacttggaa agctatggct gtgccatgaa ttttgcggac
     1201 agtgagatcg tggcgtccat cctgaaggac agcggtttta cggcttcccc cacttatttg
     1261 gatgccgacc tggtgctggt caatacctgt tccatccgcg aaaaggcgga acagacgatc
     1321 cgaaaccggc ttcacctttt ccggcaggca aaaagatcga gtcccggcat gctcatcggc
     1381 attttgggct gcatggccga aaggcttaag gcaaaattgc tggaggagga aaagctggtt
     1441 gacctggtgg tgggcccgga cggataccgt acgctcccgg ttttgatcga agcggcggaa
     1501 ggcggtcaaa aatccgtgaa cgtattgctt agccgggaag aaacgtatgc cgacatctct
     1561 cccgtaaggc tcaacagcaa tggagtgaat gcttttgtaa gtatcatgcg gggctgtaat
     1621 aatatgtgct ctttctgtgt agtgcctttc acccggggaa gggaaagaag ccgcgatgcc
     1681 cgttccatcc tcaacgaatg ccaggatctt tttgatcagg gctaccggga aattaccctg
     1741 ctcggacaaa atgtggatag ttatcactgg cattcccccg atgcggaagc cttttctttc
     1801 gcgatgttgc tggagcaggt ggcccgcata tccccgctga tgagggtgcg tttttccacc
     1861 tctcacccga aagatattac ggatgaagta ttggttgtca tgtcccggca tgaaaatatc
     1921 tgtaactata ttcacctgcc ggtgcaaagt ggatccaacc gggtgttgca gctgatgaac
     1981 cggacctata caagggagtg gtacctggct aaagtgcgga gaatccggga actgataccg
     2041 gattgtggca tcagttcgga tattattgcc gggttctgta cggaaaccga ggacgaccag
     2101 caggaaacat tatcgcttat ggaagaagcc cgcttcgaca tgagttatat gtatttctac
     2161 agtgaaagac cgggaacgct ggcagccaga agatatacgg acgatgttcc ggaagccgtg
     2221 aagaaaagaa ggctggcgga gattgtgaaa tggcagaata agatttccca gttgagcaac
     2281 cgcagagata tcggaaccat ttccaaagta ctgatcgaag gagattcgag gaagagcgat
     2341 caggactgga tgggaagaaa cagccagaac aaggtagtgg tatttcctaa acagggccat
     2401 atcctgcgca aaggggatta tgcgatactg ctgattgtgg actgcaccgg ggcaaccctg
     2461 atcggtgcgt tgatttcggg atcataaaat tcaggattca ccggcagcac cgggactgga
     2521 tcaataattg aaacaaaatg gaacttcagt cgataaaaaa caggtttggt atcatcggta
     2581 attcagctga gctgaattat gcgctgaccg tggccaccca ggtggccaat accgatctga
     2641 ccgttttgat atttggggag agcggtgtgg gcaaagaggc cttctctcat atcatccatt
     2701 ctctttcggc ccgaaaacac aatcccttta ttgccgtaaa ctgcggggcc attcccgagg
     2761 ggacgattga ctccgaatta ttcggacatg aaaaggggtc attcaccggt gcggtggatt
     2821 cccggaaagg atattttgaa acggtaaatg gaggtactat tttcctggac gagatcggag
     2881 aactgccgct gggtacgcag gcccgtctcc tccgggtgtt ggaagccgga gaattcatcc
     2941 gggtgggctc ctccaaagtg cagaaaacgg atgttcgcgt gattgccgcc accaataagg
     3001 atttgcttca gcttacggag cgcggaaaat tcagggaaga cctttattac cggttgagca
     3061 ccgtgcccat ccgggtccct tccctgagag accgcaaaga agatatcccc ctttt
//
LOCUS       DASYCU010000051         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3783189, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000051 DASYCU010000000
VERSION     DASYCU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3783189"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VGM24_01050"
     CDS             <1..995
                     /locus_tag="VGM24_01050"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4153973.1"
                     /translation="DKYLQAAKDAAAAIMAESYSLSPNFLTAYNSDNLGPDNNEVLLY
                     KRYESGLLMHSTIAYLYSSTVLSGLTKSAVESFLCTDGKPITVSPLYQGDDNIGDVVA
                     NRDERLKFTMDSTNYYYNGHSILSLTSTTGYRPMKFIPDSNKLKTIQTGINTNFTDAP
                     LFWLSEIYLIYAEAAAELDQMGEYVMTQADLDNTINKLRARGGVAPMILAGHQDPGYT
                     DPKKDADVTSLIWEIRRERRTELMMDGFRVQDLFRWKKGVYMDSNLNPDSFLGAKVPA
                     NGKIILNPDGYMQPYASNLQRTFIDPKYYLSAVPTSQILLYPPDIQAGMQNPGW"
     gene            1066..>2813
                     /locus_tag="VGM24_01055"
     CDS             1066..>2813
                     /locus_tag="VGM24_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY4153974.1"
                     /translation="MIKRFPLFLLLLCSLPAFCQTGFKFAHVSDTHIGSHNSEEDLRR
                     TVNNINADSSLQFVILTGDITDFGADSEFHTAKRILDSLNKPWHIIPGNHDANWSESG
                     TNSFRKIFGSETADFSYGGYRFLGTVCGPNMRMSPGQVSRENIVWLDSTLKHMRDPNE
                     PVIFLNHYPIDSSLNNWYEVIDLLKTGNTQLILCGHGHRNRVFDFEGIPGIMGRSNLR
                     AKEPVGGYNVVTIKDGTVTYEERNPVTNEQKKWAEVKLFRHDFAADTTHYFRPSYAVN
                     QVYKKVKTVWQFNDNSDIGSGVALSGKLVIASNSNGLIYALDLKNGKKVWSFPTQGKI
                     YSTPAVDQGLAVVASTDGIIYCLNAGNGSLAWKFETQKPIVANPLIKNGTVFIGSADG
                     HFRALNLNTGKLKWDFEGVKGFVVTRPLFYRDHIYFGSWGTEFYALDSETGKLAWKWN
                     NGSNNRMYSPAACYPVATKGRIFIVAPDRYVTVFDAETGSVIWRKRDPQYRVRESMGL
                     SANSTLVYAKTMEGELIGISTKADSMVVSWKSATGLNYEIAPTAIVEYKNLIYVPSNS
                     GLVVAVNRDGSVLWKH"
BASE COUNT          756 a          675 c          716 g          666 t
ORIGIN      
        1 ccgataaata cttgcaggcc gcaaaagatg ccgctgccgc tattatggca gaatcctaca
       61 gcctcagccc gaattttctg accgcataca attccgataa cctgggcccg gataacaatg
      121 aggtcctgct gtataaaaga tatgaatccg gattgctgat gcattcaacc atcgcgtacc
      181 tgtatagcag caccgtgctc agcgggctga ccaagtctgc cgtcgaatcc tttctctgta
      241 ccgatggcaa accgatcacg gtttctcctt tgtaccaggg cgatgataat atcggcgatg
      301 tggtcgccaa ccgggatgaa agattgaaat tcacgatgga cagcaccaat tattactata
      361 acgggcattc gatcctgagc cttacctcca ccaccgggta ccggccaatg aaattcattc
      421 ccgattccaa taagcttaaa accattcaga cggggatcaa caccaatttt accgatgctc
      481 ccctgttctg gttgtctgaa atttacctca tctatgcaga ggccgcggct gaactggatc
      541 agatgggcga atacgtgatg acccaggcgg atctggataa taccattaat aaactgaggg
      601 caagaggagg cgtggccccg atgatcctgg ccggtcacca ggatccggga tacactgacc
      661 cgaaaaagga tgcggatgtt acttcactta tctgggagat ccgccgcgaa agacgcacgg
      721 aactcatgat ggacgggttt cgcgtgcagg atctgttccg ctggaaaaaa ggcgtttata
      781 tggactcgaa tctgaatccc gattcattcc tgggcgcaaa agttccggcc aacgggaaga
      841 tcattctgaa tcccgacggg tatatgcagc cctatgcttc aaatctgcag cggactttta
      901 ttgatcccaa atattattta tcggccgttc cgacctccca gattctgttg tatcccccgg
      961 atatccaggc ggggatgcag aatccgggct ggtaatgcaa ggttttctat ggaaacagga
     1021 agtcccgcga cttcctgttt tacttttata caatatctgt agccaatgat aaaaagattt
     1081 ccgttattcc tgctgctgct ctgcagtttg cccgctttct gccagaccgg attcaaattt
     1141 gcccatgttt ccgatacgca tatcggcagt cataattcgg aagaagacct gcgaaggacc
     1201 gtaaataata ttaatgcgga ttcatcactg cagtttgtca tcctgaccgg cgacatcacg
     1261 gatttcggcg cggattcgga attccataca gccaaacgga tcctggacag tctgaacaag
     1321 ccctggcata tcattcccgg caatcacgat gcaaactggt ccgaaagcgg aaccaacagt
     1381 ttcaggaaaa tattcggttc ggaaacggct gatttctctt acgggggcta tcgattcctg
     1441 ggcaccgttt gtggtcccaa tatgcgcatg agccccggac aggtatcccg ggaaaatatc
     1501 gtttggctgg attcaacgct gaagcatatg cgcgacccga acgagcccgt cattttttta
     1561 aatcattacc cgatcgacag ctcattgaac aactggtatg aagtgattga cctcttgaaa
     1621 acaggaaata cgcaattgat attgtgcggg cacggacacc gtaaccgcgt gtttgatttt
     1681 gaaggcatac ccggcatcat gggaagatcg aatctaaggg cgaaagaacc ggtagggggt
     1741 tataatgtgg taacgataaa ggacgggacg gtcacctatg aagaacgcaa cccggttacg
     1801 aatgagcaaa agaaatgggc ggaagtgaaa ttattccggc atgatttcgc tgcggatacc
     1861 acgcattatt tccgcccgtc ttatgcggta aaccaggttt ataaaaaagt aaaaacggtc
     1921 tggcagttta atgataatag tgatatcgga tcgggtgtgg cgcttagcgg aaagctggtc
     1981 attgccagca attcgaacgg gttgatctat gccctggatt taaaaaatgg taaaaaggtc
     2041 tggtcctttc cgacacaggg aaaaatatat tccactcccg ccgtggacca gggactggcc
     2101 gtcgtggctt ccaccgatgg tataatttat tgtctgaatg cgggaaacgg atcactggca
     2161 tggaaatttg aaacgcaaaa acccatcgtg gccaatccgc tgataaaaaa cgggacggtc
     2221 tttataggtt cggcagatgg acatttcagg gcgctaaacc tgaataccgg aaaattaaaa
     2281 tgggattttg aaggcgtgaa gggatttgtc gtcacccggc ccctgtttta ccgggatcat
     2341 atttattttg gcagctgggg tactgaattc tatgcactgg acagtgaaac gggcaaactg
     2401 gcctggaaat ggaataacgg ttcgaacaac cggatgtatt ctcccgccgc ctgttacccg
     2461 gtggccacga agggcaggat ctttattgta gcgcccgacc ggtacgtgac ggtatttgac
     2521 gcggaaaccg gatccgtgat ctggaggaag agagatccgc aataccgggt aagggaatcg
     2581 atgggcctct ccgccaacag caccctggtc tacgccaaaa caatggaagg ggagttgatc
     2641 ggcatctcca caaaggcaga cagcatggtg gtatcctgga agtcggccac cggattaaac
     2701 tacgaaattg caccgacggc catcgtggaa tataaaaacc tgatctatgt gccttccaat
     2761 tcaggactgg tcgtggccgt aaaccgcgac ggatcggttt tatggaaaca caa
//
LOCUS       DASYCU010000052         5900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4508123, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000052 DASYCU010000000
VERSION     DASYCU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4508123"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..294)
                     /locus_tag="VGM24_01060"
     CDS             complement(82..294)
                     /locus_tag="VGM24_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153975.1"
                     /translation="MKTLGIVLIVLGIAMIIIRGISVPTQKKVVDIGSLEINKTENKW
                     IGWPTYAGAVVAVIGVVLTVSGKKRT"
     gene            385..2205
                     /locus_tag="VGM24_01065"
     CDS             385..2205
                     /locus_tag="VGM24_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4153976.1"
                     /translation="MVSNQLKPSWKDRLKALRNLPAFFKLVWAASAWMTIANIGLRLL
                     RSAIPVSVLYIGRLIIDEVIFLNKDHGNGPTAHLWTLIGIEFALALVSDALGRCVNLL
                     DNLLGDLFGKMSSVRIMEHAATLDLEQFEDAVFYDKLERARQQTTGRTVLLSQVLSQV
                     QDLITMGFLTVGLILFNPWLILLLFISILPSFIGESYFNDQTYALSRRQTPERRELDY
                     LRYLGASDETAKEIKIFNLSGFIIDKFKTLADRFYLSNRKIAISRSVWGTGFTLLGSL
                     GYYSAYVYIIFETIHGWISIGQLTFLAGSFRQLRSLMESVLSRFTAVSQGAIYLSDFF
                     EFFEIQPGIISGRRARPFPHPVKQGFVFENVGFRYRHAEEWATRNLNFTLKAGEKIAL
                     VGENGAGKTTLVKLLARLYEPVEGRILLDGFDLKEYDLNSLRKNIGVIFQDYLRYQMR
                     VSDNIAVGNIEEKEDKPRIISSAEQSLAADLVARLPGGYDQMLGKRFFEGVELSGGEW
                     QKVALARAYMRDAQVLILDEPTSALDARAEFEVFQRFAAITKGRTAVLISHRFSTVRM
                     ADRILVLQQGKLIESGTHEELILKKGMYAELFNLQAKGYQ"
     gene            2235..2594
                     /locus_tag="VGM24_01070"
     CDS             2235..2594
                     /locus_tag="VGM24_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HEY4153977.1"
                     /translation="MNGKPEYCPRCKKAFSCNKEDISACDCATVNPSNDTRSFLSKTF
                     YGCLCNDCLKALDQLVAREKEYPFPGPGGELMEGIHYYNEGEYRVFTEFYHVSRGYCC
                     RSGCRHCAYGFKKRYAE"
     gene            complement(2609..2836)
                     /locus_tag="VGM24_01075"
     CDS             complement(2609..2836)
                     /locus_tag="VGM24_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY4153978.1"
                     /translation="MASTRIIVKSNGSLKIEGDFEIVDLTGARYGLGGREIISICRCG
                     LSQNKPFCDGSHKGHFEHEAIAFDMAPKKTD"
     gene            complement(2876..3958)
                     /locus_tag="VGM24_01080"
     CDS             complement(2876..3958)
                     /locus_tag="VGM24_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4153979.1"
                     /translation="MKYYLLFIPTLFVFTACSYNRAADRKPEEKKTISALYRLAAIEQ
                     AGVPSVIKLPGQLAAYEEVSIYPKVNGYVKSVRVDIGSRVSKGQLLMELEAPELLQAS
                     LEAGEKYARARADFSMDKEHYQRLLEASGTPGAISPLDLSTIKSRMAADSALSNAEKN
                     NWQMQQTMQTYLKVTAPFDGVITERNVHPGALISAETKDKPMLELKQTDRLRLQVDVP
                     EGIAVNLKDKDRISFYTSALPEKKMTGFISRKSMNVNVQFRSERMEVDVMNKDGLLSP
                     GMYADVLVYSKGNPAAFRVAKSAVITTTERKYIVIVKNKKAYQTDVTTGNEANGQIEV
                     YGNLSAGDRVVVNPEDDIRNGDVVSE"
     gene            complement(3969..>5900)
                     /locus_tag="VGM24_01085"
     CDS             complement(3969..>5900)
                     /locus_tag="VGM24_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4153980.1"
                     /translation="ISFPLLLILLCILAVFAPSFVMKGVPRAMFLPLSMSIGFAMVFS
                     FIAAQTLVPVISNWLLKEDMFRYRHTGYHAHAGLALDEEELKEVKKHDRQDKIHASEN
                     GFFQRLKTRLTRRLEKWMPKRKIIVSLYLLITLAAAGLCFIFIGKDLLPKTNNGQLQI
                     RIKEPDGTRLEKTERTVKGILTLLDSTVNGHVAISSAYVGLVPSSYGTSNLYIFNSGT
                     NEAVMEVELDKSYKTNLDQLKDKLRQNIRAAYPAVNISFEPIELTEKIMAQGASTPIE
                     VRVAGKNFDHIKNYSERLIHNLKQIPYLRDVQIAQPLHFPTINMHIDRFRLAQMGLSL
                     NEVARSITDATSSSRFTEKVQWLDKKVAYTYQVQVEVPEYLMNSLEQLKSISLVKGKD
                     RPVLSDVAQFSMDTLPGEYDRSGPRRFLTVSANINHKDLGTATLAVQKAIRDLGTPPT
                     GLITQVRGMSSLLTETLNSLQTGLMVAIVVILLLLAANYQSFGLAISVLATVPAVLLG
                     AMLMLLATGATLNLQSYMGIIMSTGVSVANAILIVTNAEALRPEYKNPFKAAWVSAGI
                     RLRPILMTSIAMIAGMIPMASGIGEAGDQSAPLGAAVIGGLAASTFAALFIVPLVYGW
                     VRQKSNYEPVSLLPEASNH"
BASE COUNT         1372 a         1482 c         1525 g         1521 t
ORIGIN      
        1 agaaggccaa atagttcgga tccatcaata aacatgctgc atcaggtgat cctgctgcag
       61 catgtttttc cgtaaccggg ttcaggttct tttttttccg gataccgtta gcacaacccc
      121 aatcacggcc accacggctc cggcataagt cggccacccg atccatttat tttctgtttt
      181 gttaatttcc agggagccga tgtcgactac ttttttctga gtcggcaccg aaatgccccg
      241 gatgatgatc atggcaattc caaggacgat aagaacgatt cccaaggttt tcatgtgctg
      301 ttgttttgaa acatactgaa aacatcgtac cagccaggcc gaaacagcat tacgctgggg
      361 aatttgtagt tttgcccgga ataaatggtt tcaaatcaac tcaaaccaag ctggaaagac
      421 cggctgaaag cgcttcgcaa cctcccggct ttttttaaac tggtttgggc tgccagtgcc
      481 tggatgacca tcgccaacat cggcctgcgg ttgttgaggt cggccattcc cgtatcggtt
      541 ttatacatcg gccgcctcat tatagatgaa gtcattttcc ttaacaagga tcacggcaac
      601 gggccgacgg ctcatttgtg gactttaatc ggcattgagt ttgccctggc gctggtcagt
      661 gatgcccttg gccgctgcgt taacctgctg gataatttac tgggcgacct gttcggcaag
      721 atgagttccg tgcgcataat ggaacatgcg gccacactgg acctggaaca atttgaggac
      781 gccgtctttt atgataagct ggaaagggcg cggcagcaga ccaccggccg taccgttttg
      841 ctgtcgcagg tgctgagcca ggtacaggat ctgattacga tggggtttct gacggtcggt
      901 ctcattttgt tcaatccctg gcttatcctt ttattattca tcagcatcct gccttctttt
      961 atcggggaat cgtattttaa tgatcagacc tatgcattga gccgccggca gacaccggaa
     1021 cgccgggagc tggattacct gcgttacctg ggcgccagcg atgaaacggc caaagaaata
     1081 aagatcttca atctctcggg gttcatcatc gacaaattca aaacgctggc tgaccggttt
     1141 tacctcagca accggaagat tgcgatcagc cggtccgtgt ggggaacggg tttcacgctg
     1201 ttgggaagtc tgggttatta cagtgcctat gtctatatta tttttgaaac catacatgga
     1261 tggatcagca tcggtcaact gacctttctg gccgggtcct tccggcaatt gagatccctg
     1321 atggaatccg tcctttcccg gtttacggcc gtatcccagg gagcgatcta cctcagcgat
     1381 ttttttgaat tctttgaaat tcagcccggg atcatttccg gccgccgggc acgccctttc
     1441 ccacatccgg taaaacaggg ttttgttttt gaaaatgtcg gcttccggta ccgtcatgcg
     1501 gaagaatggg ccaccagaaa cctgaatttt acgttaaaag caggagagaa aatcgctctg
     1561 gtgggtgaaa acggggccgg gaagaccacg ctcgtaaaat tgctggccag gttatatgaa
     1621 ccggtcgaag gccggatcct cctggatgga tttgatctga aagaatatga cctgaacagc
     1681 ctgcggaaaa atatcggtgt gatctttcag gattatctgc gctaccaaat gcgcgtatcc
     1741 gataatattg cggttggaaa tatcgaagag aaggaagata agccgcgaat catttcttct
     1801 gcagaacaaa gtctggccgc cgacctggtg gcccgcctgc ccggcggtta tgatcagatg
     1861 ctgggtaaac gattttttga aggggtggaa ctttccggcg gtgaatggca aaaagtggct
     1921 ttggcgcgcg cctatatgcg ggatgcccag gtgctgatcc tggatgaacc gacttccgca
     1981 ctcgatgcgc gggctgaatt tgaagtattc cagcggtttg cggcgatcac caaaggccgg
     2041 accgccgtgc tgatctctca ccggttctcc acggttcgca tggcggaccg gatcctggtg
     2101 ttgcagcagg gtaagctgat tgaatccgga acgcacgaag aactgatact aaaaaaggga
     2161 atgtatgccg aactctttaa ccttcaggcc aaagggtatc aataaaagag tatgaaatca
     2221 atttataaca acagatgaat gggaaaccgg aatattgccc ccgttgcaaa aaagctttct
     2281 cctgtaataa ggaggatatc agtgcatgcg attgtgcaac cgttaatcca agcaatgaca
     2341 cccggagctt cctgtcaaaa acgttttatg gctgcctttg caatgactgt ctcaaggccc
     2401 tggaccaact ggttgcccgc gaaaaagaat atccttttcc cggtccgggc ggggaactga
     2461 tggaggggat ccattattac aatgaagggg aataccgggt attcacggaa ttttatcatg
     2521 tgtcccgtgg gtattgctgc cggagtggct gccggcactg cgcctatggg tttaagaaac
     2581 ggtatgcgga atgatggatg cccgaaaatc aatccgtttt cttcggggcc atgtcaaatg
     2641 caatggcttc atgttcgaaa tggcccttgt gggaaccgtc gcagaaaggt ttgttttgcg
     2701 ataacccgca acggcaaatg gaaatgatct ctcttccgcc gagcccgtac cgggcccccg
     2761 tcaggtccac gatttcaaaa tccccttcaa ttttcagaga gccattgctt tttacgataa
     2821 tccgggtact ggccatagtg ttattctgat tttgaatgaa attaggaaaa ctatttcatt
     2881 cactcaccac atcgccattc cggatatcat cctccgggtt aaccaccacc cggtcgcctg
     2941 cgcttagatt tccgtaaact tcaatttgcc cgttggcttc attgccggtg gttacgtcgg
     3001 tctggtatgc tttcttattt ttaacgatta caatatattt tctttcggtg gtagtgataa
     3061 cggccgattt agccacccgg aatgcagcgg ggtttccttt tgaatagacc agtacgtcgg
     3121 catacattcc gggggatagc aatccgtcct tattcattac atccacctcc attctttcag
     3181 aacggaactg gacgttcaca ttcatggatt tccggctgat gaaaccggtc atttttttct
     3241 cgggcaaggc gctggtgtaa aacgaaatgc ggtccttgtc tttcaggttg acggcaatgc
     3301 cctccggcac gtccacctgc agccgtaaac ggtctgtctg cttgagttcc agcatcggct
     3361 tatcttttgt ttccgcgctg atcagggcgc ccggatgaac gttgcgttcg gtaatcacgc
     3421 cgtcaaaggg ggcggtgacc ttcaggtagg tttgcatcgt ttgctgcatc tgccagttat
     3481 ttttttcagc attgctgagc gcgctgtccg ccgccatcct ggattttatc gtcgacaaat
     3541 ccaacggcga tatggctccc ggagtgccgg aggcttccag tagtcgctgg taatgttcct
     3601 tatccattga aaaatcggcc cgggcgcggg catatttttc accggcttcc aggctggcct
     3661 gcagcaattc cggtgcttcc agctccatga gcagctggcc cttggatacc ctggaaccta
     3721 tatccacccg caccgatttt acatatccgt tcactttggg gtagatactc acttcttcat
     3781 aagcggccag ctggccgggt agcttgatca cggaaggcac tcccgcctgt tcgatggcgg
     3841 ctaaccggta taaagcggaa atggtttttt tctcttccgg tttccggtct gctgcccggt
     3901 tataggaaca ggcagtgaaa acaaaaaggg taggaatgaa gagcagataa tatttcatga
     3961 taagtatttt aatgattgga agcctccggt aataaggata cgggttcata atttgatttt
     4021 tggcgcaccc atccatacac cagcggtaca ataaacaggg ctgcaaaagt ggatgctgcc
     4081 agtccgccga tcacggcggc tccgagtggg gcagactggt cgccggcttc gccgatgcca
     4141 ctggccatgg ggatcatgcc ggcgatcatg gcaatactgg tcattaatat cggtcgcaac
     4201 cggatgcccg cgctcaccca ggctgctttg aatgggtttt tatattcagg ccgcaacgct
     4261 tccgcattgg ttacgatcag aatggcgttg gccacggaaa caccggtcga cataatgatg
     4321 cccatatagg actgcaggtt cagcgtggca cccgtggcga gcagcataag cattgcgcct
     4381 aataaaacgg ccggcaccgt agccagaacg gaaatggcta aaccgaagga ttgatagttg
     4441 gcggccagca gcaaaaggat gaccacaatg gccaccatca gcccggtctg cagggaattc
     4501 agggtttccg ttaacagaga agacatgccc cggacctgcg tgatcagccc ggtgggcggc
     4561 gttcccagat cgcggatcgc tttctgtacg gcaagggtag cggttcccag gtctttatga
     4621 ttgatattgg cactcactgt aaggaatcgc ctgggcccag accggtcgta ctctcccgga
     4681 agcgtatcca tgctgaactg cgccacgtca gacagcacag gccggtcctt tccttttacc
     4741 agtgaaatgg atttcagctg ttccagggaa ttcatcaggt attccggaac ttcaacctgt
     4801 acctggtagg tgtacgccac ttttttgtcc agccattgca ccttctccgt gaaacggctg
     4861 gaggaagtgg catcggtaat gcttcgggcc acctcattca ggctcagccc catctgtgcc
     4921 agacggaaac ggtcaatatg catgttgatg gtcgggaaat gcaggggctg ggctatctga
     4981 acatcccgca ggtaagggat ctgtttcaga ttatggatca gcctttcaga atagtttttt
     5041 atgtgatcga aatttttgcc tgccacccgc acttcgatcg gcgttgaagc cccctgggcc
     5101 atgatttttt ccgtaagctc gatgggttcg aatgaaatat tcactgccgg ataagcggca
     5161 cgaatatttt gcctcagctt atctttcagc tgatccaggt tcgttttata ggatttgtcc
     5221 aactccactt ccatgaccgc ttcgttggtt ccgctattga aaatataaag attgctggtc
     5281 ccgtaactgc tgggaaccag tcccacatag gcggagctga tcgccacatg cccgttcacg
     5341 gttgaatcga gtaaagttaa aatgccttta accgtccttt ctgttttctc cagccgggtg
     5401 ccatccggtt ctttaatccg gatctgcaac tgcccgttat tggttttggg cagcaggtcc
     5461 tttccgataa aaataaaaca aaggccggcc gccgcgagcg tgatgagcag gtagagactg
     5521 acaatgattt tccttttagg catccatttt tccagtcttc gcgtgagacg cgttttcagc
     5581 cgctgaaaga atccgttctc cgaggcatgg atcttgtcct gacggtcatg tttttttact
     5641 tctttcaatt cctcctcgtc cagggccagg cccgcatgcg catgataacc ggtatgccgg
     5701 taacggaaca tatcctcttt cagcagccag ttggaaatga ccggcaccag ggtctgcgcc
     5761 gcaataaacg aaaagaccat ggcaaagccg atggacatgg acaggggaag gaacatcgcc
     5821 ctgggcacgc ccttcatcac aaaggaaggt gcaaataccg ccaggatgca taataagatc
     5881 agcaggagcg ggaaagagat
//
LOCUS       DASYCU010000053         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_594825, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000053 DASYCU010000000
VERSION     DASYCU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594825"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VGM24_01090"
     CDS             <1..1377
                     /locus_tag="VGM24_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY4153981.1"
                     /translation="WSDAFSLGSDYRASLALNPFQSFFSSLKFRKSGYRLNEVKQAYP
                     LLKPYYGFTGDSADLNYDRFTRTVPGSASPNIVVVICESFSAYKSSMWGNPLNTTPFF
                     KGMTDNGIFFDRCITPSYGTARGVWAVLTGIPDVGQANSTTSRNPAAVDQHSIVNDFT
                     GYEKFYFLGGSASWANIRGLLTDNIKGLHLYEQPDYKAPKIDVWGISDKNLFLEANQV
                     LNKQTKPFIAIIQTADNHRPYTIPAEDEAAFHQIRVSADSLKQFGFESNEEMNAFRYT
                     DFCYQKFIGAARAEKYFSNTIFVFVGDHGIAGNAGKMFPEAWTEERLDNMHVPLLFYA
                     PSLLKPQRIHEFVSQVDLLPTAAGLAKISYHNTSLGRDLLDSAQAPAKAFSFIYDPDQ
                     GYIGLLMGDYLYREQMTTHKGQLYSAVSNDPVDPVLSKDSLAKMAALTRGIYQTSLYL
                     LLHNKKKP"
     gene            1385..>1956
                     /locus_tag="VGM24_01095"
     CDS             1385..>1956
                     /locus_tag="VGM24_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153982.1"
                     /translation="MDTSRLVLFSFLALCFSCGNRRQEKNINVPGKIRKIPVTRDQVY
                     DLSGYADGRGNPFNLFDENAYVDPRYTRAGEPYIPVTDCQPTLHPEIYFPAGIGSRIV
                     VDLLVPYDLKEIYLYDRSSVSDSVWIYSGTMKHWQQITALETASEPGSRGWKKISADI
                     RSRFVMIRFSSNETRITEMVLYGTAAAAIPP"
BASE COUNT          461 a          534 c          500 g          461 t
ORIGIN      
        1 tggagcgatg cgttttccct gggcagtgac taccgggcca gcctggcatt aaatcccttc
       61 cagtcttttt tcagcagcct gaaattccgt aaatcgggct accgcctgaa tgaagtgaag
      121 caggcttacc cgctgctgaa accttattac gggtttaccg gcgattctgc ggatctcaac
      181 tatgaccggt tcacccggac cgttccggga tctgccagtc ccaatattgt agtggtcata
      241 tgcgaatcct tcagtgctta caaaagttcc atgtggggaa acccgctgaa cacgacgcct
      301 ttttttaaag ggatgaccga taatgggatt ttctttgacc gctgcattac gccttcctat
      361 ggaacggcgc gcggcgtatg ggccgtcctg accgggattc ccgatgtggg gcaggccaat
      421 agcacgacca gccgaaatcc ggcggcggtg gaccagcaca gcatcgtgaa tgattttacg
      481 ggatacgaaa aattctattt ccttggcggg agcgccagct gggcgaatat ccggggcctg
      541 ctcaccgata acattaaagg acttcacctg tacgagcaac cggattacaa agcgcccaag
      601 atagatgtgt ggggaatcag cgacaagaat ttatttcttg aagccaacca agtgctgaac
      661 aaacagacca aaccttttat tgcgatcatt caaacggccg acaaccatcg tccctatacc
      721 attccggcgg aggatgaggc cgccttccac cagatccggg tatcggcgga ttccctcaaa
      781 cagttcgggt ttgaaagcaa tgaagaaatg aatgccttcc gctataccga tttctgttac
      841 cagaaattca tcggggccgc ccgggcggaa aaatatttct ccaacacgat ttttgttttt
      901 gtcggcgacc acgggattgc aggaaatgcc ggaaagatgt ttccggaggc ctggacggag
      961 gagcgcctgg ataatatgca tgtgcccctg ctgttttatg ctccgtcctt attaaaaccc
     1021 caacggatcc acgaattcgt ttcccaggtg gacctgttgc cgacggcggc cggcctcgct
     1081 aaaatttcct atcacaatac gagccttggc agggatctgc tggattcggc gcaagcgccc
     1141 gcaaaagcgt tttcttttat ttatgatccg gatcagggct atatcggcct ccttatgggg
     1201 gactacctgt accgggaaca gatgaccacg cataagggac agctctattc cgccgtttcc
     1261 aacgacccgg tggatccggt gctgtcgaaa gacagcctgg cgaaaatggc tgccctcacc
     1321 cgcgggatct atcaaacctc cctgtacctg ctgctgcaca ataaaaagaa accctgattt
     1381 cagtatggat acctcgcgcc tggtcctttt ctcttttctg gcattgtgtt tttcctgcgg
     1441 aaaccgtcgg caggagaaaa atataaatgt gcccgggaag atccggaaaa tccctgtcac
     1501 ccgcgaccag gtatacgatc tcagcggata cgccgatggc cggggcaatc cctttaacct
     1561 gtttgatgaa aatgcctatg tggatccccg ttatactcgc gcgggagagc cctatatccc
     1621 ggtgaccgac tgccagccca cccttcatcc tgaaatttat ttccctgccg gcatcggaag
     1681 ccggatcgtg gtcgacctgc tggtgcctta cgatctgaaa gaaatttatt tgtacgaccg
     1741 gtcttccgta agcgatagcg tatggatcta ttccggcacc atgaaacact ggcagcagat
     1801 cactgccctg gaaacggcct cggaaccggg atcccgcggc tggaaaaaga tcagcgcgga
     1861 tatccgttcc cgttttgtca tgatccgctt cagcagtaat gaaaccagga tcacggaaat
     1921 ggttttgtat ggaacggcgg cagcggccat tccccc
//
LOCUS       DASYCU010000054         7977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2188684, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000054 DASYCU010000000
VERSION     DASYCU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2188684"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VGM24_01100"
     CDS             complement(<1..520)
                     /locus_tag="VGM24_01100"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEY4153983.1"
                     /translation="MIKSTLKYCLGLSVLFLLLTVTGNGQTNSPKSSDEGWTQLFNGK
                     DLTGWGFSPTSDSDKESARKSHESDPNAPAWPIVNQRVDFDGQQTSSDGRFIAKNGRL
                     IVAIPSEGRRIQELSTRQTFPKDFILRLEFRAVPNADSGIFIRGEQLQCRDYPLAGPY
                     KKLKNYRPGDWNE"
     gene            644..1717
                     /locus_tag="VGM24_01105"
     CDS             644..1717
                     /locus_tag="VGM24_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4153984.1"
                     /translation="MTKKNATPLAGVKEIARLAGVSIATVDRVIHKREGVSAKTKKKI
                     EAIIGKLNYQPNILAQRLALASRGTIRLAVLLPNISQETEFWMAPLEGIRQAQGEIKR
                     YNVHVENYFFDQNDQQSFVKQMKALAKYKTDGILFTPTFPEESLQFIESLEKAECPYV
                     LINCDIPGHDSLGYIGPDILHSGYLAGQLLHYCIKGGEKILIVNISGEIDNNSAILRK
                     EEGFRRYFKENGLENELLSFNTTQTDYSSVSRKLDALLKKQKGVEAIFVTNSRVSVLA
                     RYLEKAGLNDMLLLGYDFVKDNVTYLKKGVIDFLICEKPQEQGYRGIMTLFQHLVFSS
                     PIEKKYLMPIDIITKENCQFYRN"
     gene            1722..3611
                     /locus_tag="VGM24_01110"
     CDS             1722..3611
                     /locus_tag="VGM24_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153985.1"
                     /translation="MNTIPSRTARFLPVIFLLFITPRIHAQELALKKEYAKSLIEWSD
                     ALLKRQMTTPGDPNRGGIWCAHCRVWHTRAAESVFPFSVCWSITKDARYLKGAKDLAA
                     WLIRQQQPDGSWKETPEEWTGTTTDQLLMLLMSYEMISPQLNKKEQSDWQQSMKKAAD
                     YLARVMRPEFASINYVATTTATLAKAGLVFKADSYLQKARELARRTISKMDEDGFLNG
                     EGGKSHGNKMGVDLGYDMEMSLWGLGLYARLTGDTLVNSAVRKALINHLYFIYPDGSM
                     DGSWGIRSNKWTGYGGATSDGCQVLFSLFADDDPRYAAAGWRNLRFLRTNTVQDLIGY
                     GPQHAELFDGPPCIYPTFAKAKNLAMAYVFETHEKRPMPSLPVDETGWMKLFPTLNVV
                     EVRTANFMATITGYHYKDQFAGWKGKYMYRPDGGAMSHVWLKDHGFLQASSPTVYTRP
                     EPMSFPEAPGVVSLTPRIEYSDTAGYFTNLYEFDSRLDTSRNSKGEFEITARGELKDK
                     YWLTGGVSYRMDYLIADRFYQKTIRLTYHDAWPLVKIIEPFIDFKGMKFSPVDDQSME
                     IQAGRRKLLFRILTPGVRLIAGRDKEKYWTPYPALRAFPLEMELKPEPGHFIQTVTYR
                     LSVEE"
     gene            3708..4646
                     /locus_tag="VGM24_01115"
     CDS             3708..4646
                     /locus_tag="VGM24_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY4153986.1"
                     /translation="MNNQPYPDQITCAYLYPITKYGYPPDIRETVEHIGEMSAMGFRS
                     IELEGIGEENILYLYRNHDRIGEELARTSCRVPVLCVVLPQLSGVDALQRARALELFE
                     MGCSVAQRLGAGFVLDNGPLLPMQSPAGAPIRRHYAGDAQAVREILSGKSWEEYWHEL
                     VSTYREACGIAAKFNLGYYLHPCEGSLITGTDSFLHFSQAVNCGNLLFNLDTANQFYF
                     KDNLQLSVLRLAGKTGYIHISDNSGLRVEHLVPGDGNIQWDLFFSALQDTGFKGRFAI
                     DIGGAETHIPDIRKAFLRCADWLEEQLIRYSLNNQS"
     gene            4643..6190
                     /locus_tag="VGM24_01120"
     CDS             4643..6190
                     /locus_tag="VGM24_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEY4153987.1"
                     /translation="MKPGYFFLMAGRVLCFTGMILGLGLTVHAQFFDVTAFGAVGDGK
                     TDNTFSIQRAIDSCAQKGGQVYFPAGKFLSATLHLKNRVTLYLSGGATLLGQPDPKKY
                     PYLDAGIRFYGDAWARQSLIFCRDQENVAIEGSGTIDGQGAKFVVTTLKKPDRYRDRP
                     YLLWFAGCRNVSVKGVQLRNSAYWMQHYLGCDGVKIDGINIWNHSNKNNDMMDIDGCR
                     NVIVSNVTGDSDDDGITIKSTSPLISEYISITNCLISSHCNAIKFGTESTGGFRNVVI
                     SNCVIRPSAQHTTIYGRPAGMSGLALEVVDGGVMENISIDHILISGPQVPFFVRLGNR
                     ARKYKEGVAEPTAGSIKNIHLSNIVATGADETGCFLTGLPNAPLEDIFLNNISMETKG
                     SDSVFDLNRPVEEMETAYPEGTMFGKLPAFGFFIRHAKGVNVSDISIHSLNRETRPGI
                     LVSNTSRFSLSGLDIQPHGQTKALVYIAHSSEGTVIPEAVFPAGRPFVLKDKYSEKIT
                     VGKIVNQ"
     gene            6191..>7977
                     /locus_tag="VGM24_01125"
     CDS             6191..>7977
                     /locus_tag="VGM24_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4153988.1"
                     /translation="MKYLWLAYFFVSCCATACFPKQPASDFLFDAGPDSSGTDRYFVK
                     INASDLYQVKKGFGWVVPPRQIFAGANGKIPQRLLHDGVLTTDSMVFRADLPDGNYFM
                     RLFLGVPAPDSSKMIIAVNGETLRDTVETPWYRIPYRTICRKLSIRHGQVQIRIRALS
                     GMSAGLYGVEFRRATENEPIPFKTALGQDSTEIGNFAHSLEKQIAAHPHDIALINQLN
                     NIDNYLLACYDYDCGGWSRFVKKTGFSLIYRMYSAADLLEPIVADTTDPLYDKSLYLL
                     AKIYYWLDQEDDNLYHSIKFNSYFSKLKNKYPGDTLIRMYLGEKIFDPPAFDTTAGNA
                     PKWAVYERETMKRMLKEINWWVNERQIPDGEMGGKYGDDVELLDWWLPAILGADDSTA
                     RKGYQRLADGVWHSDAMERGFAKRLDDVEHSAELFRDTHPAMFLLNYGDPEYVERCLI
                     SMQNFRDVWTGITPLGHLHFKSYYLSATRTLSGPDSGIDVALNARAILPGLWAAWYNH
                     NPTLIKLFSAWGYSWISDADRKDNGKPAGLMPSAVSFAQDRIGGNSNEWYDPQLSYDY
                     YLWDHLGHVGELFNHLVGMYGITGNAFFL"
BASE COUNT         1983 a         1990 c         2149 g         1855 t
ORIGIN      
        1 tttcattcca atcacccggg cgatagttct tgagtttctt gtaggggccg gccaggggat
       61 aatcccggca ctgcaattgt tcgccgcgga tgaaaatacc gctgtcggca ttgggaacgg
      121 cccggaactc caggcggaga atgaaatcct tgggaaaggt ttggcgagtg gagagctcct
      181 gaattcgacg gccttcggac ggaatggcca caatcaaacg cccgtttttg gctataaacc
      241 ggccatccga actggtctgc tgcccgtcaa agtccacccg ctgattaaca attggccacg
      301 ctggcgcgtt cggatccgat tcatgcgatt ttcgggcgga ctccttatcg gaatccgaag
      361 tcgggctgaa accccagccg gtaagatcct ttccgttgaa aagctgtgtc catccttcgt
      421 cggaagattt gggtgaattc gtttggccgt tacccgtaac ggtcagtaac agaaataata
      481 cgcttaaacc caggcaatac tttagtgttg atttgatcat ggcatttgaa attgactata
      541 aatctaagca taaaaaagat atcttcttca aagcggtaag gagttcgccg caacaaactg
      601 aggatttatt ctatagattt gttgcgaaac ctcgattcag tttatgacta aaaagaatgc
      661 aaccccgttg gccggagtaa aggaaattgc ccgcctggcc ggggtatcca tcgccacggt
      721 agaccgggtt attcataaaa gagaaggggt ttctgcaaaa acaaaaaaga agatagaggc
      781 catcatcggg aagctgaact accagccgaa tatcctcgcg caacggcttg cgctggcttc
      841 gcggggaacg atccggctgg ccgtcctcct gccgaatatt tcgcaggaaa ccgaattctg
      901 gatggctccg ctggaaggca tccggcaggc ccagggggag atcaaacgat acaatgtgca
      961 tgttgaaaat tattttttcg accagaacga ccagcagtcg ttcgtgaaac agatgaaagc
     1021 gctcgcgaaa tacaagaccg acggcattct gttcacgccg actttcccgg aggaatcgct
     1081 gcagttcatt gagagcctgg aaaaggcaga atgcccttat gttttaatca attgcgacat
     1141 ccccgggcac gacagcctcg gttatatcgg tcccgatatt ctgcacagcg gatacctggc
     1201 gggtcagttg ctccattatt gcataaaggg cggggagaag atcctgatcg tgaatatttc
     1261 cggtgagatc gacaataact ccgccatcct gcggaaggaa gaagggttcc ggaggtattt
     1321 taaagagaac ggcctggaga atgagctcct ttccttcaat accacgcaaa ccgattattc
     1381 ctctgtttcc cgcaaactgg atgcgcttct gaaaaagcag aaaggggtcg aagcgatctt
     1441 tgtaaccaat tcccgcgtgt cggtgctggc ccgttatctt gaaaaggccg ggctgaatga
     1501 tatgcttctg ctgggatatg attttgtaaa ggacaacgtc acctacctga aaaaaggagt
     1561 gatcgatttc ctgatctgcg aaaaacctca ggagcaggga taccggggga tcatgacgct
     1621 tttccagcac ctggtctttt ccagtccgat cgaaaaaaaa tacctgatgc ccattgatat
     1681 tatcacgaaa gagaattgcc agttttaccg caattaagaa catgaatacc ataccctccc
     1741 ggacggcccg gtttcttccg gtcatttttt tattattcat tactccgcgc atccacgcgc
     1801 aggaactggc tttgaagaag gagtatgcca aatccctgat cgaatggtca gatgcgctct
     1861 tgaaaagaca gatgacgacc cccggcgatc caaaccgggg cgggatctgg tgcgcccatt
     1921 gccgggtctg gcacacccgg gccgctgaaa gcgtatttcc cttttccgtc tgctggtcga
     1981 ttaccaaaga tgcgcgttat cttaagggcg caaaggatct ggcggcctgg ctgatccgtc
     2041 agcagcaacc ggatggaagc tggaaagaaa caccggaaga gtggacgggc acgacgacgg
     2101 atcagttgct gatgctgttg atgtcctatg aaatgatctc tccgcagctg aataaaaaag
     2161 agcagtcgga ctggcagcag tcgatgaaga aggcggccga ttatctcgcg cgggtgatgc
     2221 gtcccgaatt tgccagcatc aattatgtgg caaccaccac ggctacgctg gccaaggcag
     2281 ggctggtctt taaggccgat tcctacctgc aaaaagcaag ggagctggcg cgtcgcacga
     2341 tttccaaaat ggacgaagat ggtttcctca atggcgaggg cggcaagagt cacggcaaca
     2401 agatgggcgt tgacctgggc tacgatatgg aaatgtcctt atggggcctg ggtctttatg
     2461 cgcgtttaac cggcgatacg ctggtgaaca gtgccgtccg gaaagccctt ataaatcatc
     2521 tttattttat ctatcccgat ggttcgatgg atgggtcctg gggcatccgc tcaaacaaat
     2581 ggacaggata tggcggagcc acttccgacg gctgccaggt tttattttca ctcttcgccg
     2641 atgacgatcc gcgatatgcc gcggctggct ggcgcaacct ccggtttctc cgtaccaata
     2701 ccgttcagga cctgatcggc tacgggccgc agcatgcgga attgttcgac gggccgccct
     2761 gcatctatcc gacttttgca aaggcgaaga acctggcgat ggcctatgta ttcgaaaccc
     2821 atgaaaaaag accgatgcct tcactacccg tggatgaaac cgggtggatg aaattatttc
     2881 ctaccctgaa tgtggtcgaa gtgcgcaccg ctaatttcat ggcaacgatt accgggtatc
     2941 attacaaaga ccagttcgcc ggatggaagg gaaaatacat gtaccgtccc gatggcggcg
     3001 ccatgagtca tgtgtggctg aaagatcatg gatttctcca ggcttccagt ccgacagttt
     3061 atactcgtcc cgaacccatg agctttccgg aagcgccggg ggttgtatcg cttaccccac
     3121 ggatcgaata cagcgacacg gcgggctatt ttacaaacct ctacgaattc gacagccgcc
     3181 tggatacatc gaggaacagc aaaggggaat ttgagatcac cgcccgggga gaattgaaag
     3241 ataagtactg gctcacggga ggcgtaagct atcgaatgga ttaccttatc gcagaccgtt
     3301 tttatcaaaa gaccatccgg ctcacgtatc acgatgcctg gccgctggtg aagatcattg
     3361 aacccttcat tgattttaag ggaatgaagt ttagtccggt ggatgatcag agcatggaga
     3421 tacaggccgg ccggcgaaaa ttattattcc ggatactgac acccggcgtc cggttgatcg
     3481 ccggccgcga taaagaaaaa tactggaccc cgtacccggc gctccgggcc tttccgctgg
     3541 agatggagct gaagccggag cccggtcatt ttatacagac cgtcacctac cgtctgtccg
     3601 tcgaggaata aaaccggatc ccgtctgctc ccaaaaaaga tttttccccg gttttacgtg
     3661 tacgtacccg gaaaaccgtt agtttcgttc tcactaagga gccgtacatg aataatcagc
     3721 catatccgga tcagatcacc tgcgcatatc tttacccgat caccaaatac ggttacccgc
     3781 cggatatccg ggaaaccgtt gaacatatcg gtgaaatgtc ggccatggga ttccgttcca
     3841 ttgaactgga agggatcgga gaagaaaata ttctttatct gtaccgcaat cacgatcgca
     3901 tcggggaaga gctggccaga acatcctgcc gggttcctgt tctttgtgtg gtattgcccc
     3961 agttgagcgg cgtggatgcg ttgcagcggg cgcgcgccct ggaattgttt gaaatgggat
     4021 gcagcgtcgc gcagcggctg ggtgcgggtt ttgtattgga caatggcccg ttgctgccca
     4081 tgcaatctcc tgccggggcg ccgatccgcc ggcactatgc cggagatgcc caggcggtgc
     4141 gtgaaatatt atccggaaaa tcctgggaag aatattggca tgaactcgta tcaacgtacc
     4201 gggaggcctg cgggattgcc gcgaaattca atctcggata ttatttacat ccctgtgaag
     4261 gaagtcttat cacgggaacg gattccttcc ttcacttttc gcaggcggtg aactgcggaa
     4321 atctcctgtt caatctcgat acggccaatc aattctattt taaggacaac cttcaactga
     4381 gcgtcctgcg gctggcgggg aagacgggtt atattcatat ctcggataac agcggactcc
     4441 gggtggaaca cctggtaccg ggagacggaa atattcagtg ggaccttttt ttttccgcgc
     4501 tgcaggatac cgggttcaaa ggacgattcg ctattgatat cggcggggcg gagacccata
     4561 tcccggatat ccggaaagcc tttctgcgtt gcgcggactg gctggaagag caactgatcc
     4621 gctattcttt aaataatcag tcatgaaacc cggatatttt tttttaatgg caggccgcgt
     4681 tttgtgcttt accggtatga tcctgggtct ggggcttacg gttcatgccc agttttttga
     4741 cgttacggca tttggcgccg tcggcgacgg aaaaacggac aatacttttt cgatacagcg
     4801 ggcaatagac agctgtgcac aaaagggcgg acaggtttat ttccccgcgg gaaaattttt
     4861 atccgccacg cttcatctca aaaaccgggt caccctgtat ttgtccggcg gagcaacgct
     4921 tctcggccaa cccgatccga aaaaatatcc ttacctggat gccggcatcc ggttttatgg
     4981 cgacgcctgg gccaggcaga gcctgatctt ttgccgtgat caggaaaatg tggccattga
     5041 aggaagtggt acgatcgacg ggcagggagc aaaattcgta gtgaccacgc tgaaaaaacc
     5101 ggatcgttac cgggaccgcc cctacctgct ctggtttgcc ggctgccgga atgtttccgt
     5161 aaaaggggtg cagctccgga attccgctta ctggatgcag cactacctgg gatgcgacgg
     5221 agtgaaaata gatggcataa acatctggaa tcattccaac aagaataacg acatgatgga
     5281 tatcgatggc tgccggaatg tgatcgtttc gaacgtgacc ggcgactcgg atgatgacgg
     5341 catcaccatc aagagcacat cacccttgat ttcggaatac atcagcatta cgaattgcct
     5401 gatcagcagc cattgcaatg caataaaatt cggcacggag tccaccggcg gattcaggaa
     5461 tgtggtcata tccaattgtg tgatccggcc atccgcgcag catacgacca tatatggccg
     5521 gcccgccgga atgagcggcc tggccctcga agtagtggac ggcggcgtta tggagaatat
     5581 cagtatcgac cacatcctga tctcggggcc gcaggtgccc ttcttcgtcc ggctgggcaa
     5641 ccgggcccgg aaatacaaag agggggttgc agaacccact gccggcagca taaaaaatat
     5701 tcatctatcc aatatcgtgg caaccggcgc agatgaaacc ggatgctttc ttacgggcct
     5761 tcccaatgct ccgctggaag atatattctt aaacaatatc agcatggaga caaaaggcag
     5821 cgacagtgtc ttcgacctga accggcctgt ggaggaaatg gaaaccgcat atccggaagg
     5881 cacgatgttc ggcaagcttc ccgctttcgg ctttttcata cgccatgcaa aaggagtgaa
     5941 cgtgtccgat atcagtatcc attcccttaa ccgcgaaacc cgcccgggaa tcctggtttc
     6001 caataccagc cggttctccc tgtcgggtct ggacattcag ccgcatggtc agacaaaggc
     6061 cctggtctat atcgcacata gcagtgaagg aacggtgatc ccggaggcgg tctttcctgc
     6121 cgggcggccg tttgttttga aagataaata ttcagaaaaa ataaccgtcg ggaaaattgt
     6181 aaatcagtaa atgaaatatc tgtggctggc atatttcttc gtcagctgtt gtgcaacagc
     6241 ctgctttccg aagcaacccg catccgattt tctttttgat gccgggccgg acagttcagg
     6301 caccgacaga tattttgtaa aaatcaacgc ttcggatctg taccaggtta aaaaaggatt
     6361 tggctgggtt gttcctccgc ggcaaatttt tgcgggcgca aacggaaaaa taccgcaacg
     6421 cctgctgcac gacggggtgc ttaccaccga cagcatggtt ttcagggccg atctcccgga
     6481 cggaaattat tttatgcgct tattcctggg tgtgcctgcc ccggattcat cgaaaatgat
     6541 cattgcagta aatggggaga cgctgcggga taccgttgaa acgccgtggt accgcattcc
     6601 gtaccggacc atttgccgaa agctgagtat ccggcatggg caggtgcaga tcaggatcag
     6661 ggccttgtcc ggaatgtccg ccgggctgta tggggtcgag ttccggaggg caacggaaaa
     6721 tgaaccgata cctttcaaga cggcgctcgg acaggacagc acggagatcg ggaattttgc
     6781 gcattccctg gaaaaacaaa tcgctgccca tccccatgat atagccctga tcaatcaatt
     6841 gaacaatatc gacaattacc tcctggcctg ttacgattat gattgcgggg gctggtcccg
     6901 gtttgttaag aagaccggct tcagcttaat atacaggatg tattccgcag ccgatctgct
     6961 ggagcccatt gtagcggata ccaccgaccc gctctacgac aaatcgcttt atctgctggc
     7021 taaaatatat tactggctgg accaggaaga cgacaatctt tatcatagta tcaaattcaa
     7081 ttcctacttc tccaaactaa aaaataaata tcccggggat acgctgatcc gcatgtacct
     7141 gggagaaaag atattcgatc cgccggcatt tgatacaacc gcggggaacg cgcccaaatg
     7201 ggccgtctat gagcgggaaa ccatgaagcg gatgttgaaa gaaattaact ggtgggtgaa
     7261 tgagcgtcaa attccggacg gcgaaatggg cggaaaatac ggagatgatg tggaattatt
     7321 ggactggtgg ctgccggcca tactcggcgc cgatgattcc accgcaagga aaggatatca
     7381 acggctggcc gacggcgtat ggcacagcga tgcaatggag aggggatttg cgaaacggct
     7441 ggatgatgtg gaacattctg cggaattgtt cagggatacg catccggcca tgttcctcct
     7501 gaattatggc gatccggaat atgttgaacg ttgtctgatc agtatgcaaa acttccggga
     7561 cgtatggacc gggatcactc ccctgggtca tcttcatttc aaatcctact acctgtctgc
     7621 cacccggaca ctgtcggggc ccgacagcgg gatagatgtg gccctgaatg ccagggctat
     7681 ccttccgggt ctgtgggcag cctggtataa tcacaatccg acgctgataa aattattttc
     7741 tgcctggggt tattcctgga tcagtgatgc ggacagaaaa gataatggca aacccgccgg
     7801 cctgatgccc tctgcggtca gcttcgcgca ggaccggatc ggcggcaatt cgaatgaatg
     7861 gtacgatccg cagctcagct atgattatta cctgtgggat cacctcggcc atgtggggga
     7921 attgtttaat cacctcgtgg gcatgtatgg tattacgggg aacgcgtttt tcctgaa
//
LOCUS       DASYCU010000055         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1899492, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000055 DASYCU010000000
VERSION     DASYCU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899492"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VGM24_01130"
     CDS             complement(<1..1153)
                     /locus_tag="VGM24_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4153989.1"
                     /translation="MTRIAMLGSGFIGRFYADSLQGQRSRDRIVTVYSRREESAKKFA
                     SDYGVSHWTINMEEAIAHPETDVVCISLPNNLHEAAVLLCCKHKKAVITTKPLGRTAA
                     EAKRMLEAVEKAGIFNGYLEDLVYTPKFLKAYESVKNGALGRILWSKSRETHPGPHSD
                     WFWDLEQAGGGCILDLGCHCVEIGRNFIGKDIKPVEVMCWADTQVKPIEAEDHAIGLV
                     KYENGAIGQFEVSWTFRGGLDLRDEVMGTEGTIWINNFLRTGLEMFTTGKGADYVAEK
                     AESNTGWLFPVGDELNDLGYNHMFADMFNAMEKGSAPKETFYDGYIVNAVLDAAYESA
                     KTKLWEPVQLDIWRGRTGVSKDSHLVEYDADHYLVKEEMTHYGAKKVIVK"
     gene            complement(1210..2412)
                     /locus_tag="VGM24_01135"
     CDS             complement(1210..2412)
                     /locus_tag="VGM24_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HEY4153990.1"
                     /translation="MKPVTTFLLCLMMFLEYFVWGAWYVTMSTYMGANLHSTGVQIGA
                     AYSALAIATMISPFFIGLIADRYFAAQRIMGVLHLIGAVLLFLASSVTVNAPFYWIIL
                     FYSLLYMPTIALSNSIAFYQMSDPGRQFPWIRVFGTLGWIVAGLLIGNLQWEKTANTF
                     YLAAAASAGIGLISFILPNTPPKARSTEVSAAKALGTDAFVLFKNKAYLIFIIAAVLV
                     CIPLSFYYGFANAFLNEIGLHNAASKMILGQISEGVFILAIPFLFNRIGVKNMLLMGM
                     TAWILRYVCFAYGDLSSNLWMLYAGILLHGVCYDFFFVTGYMYTEKKAGAGIKNAAQG
                     LFTFATYGVGMFIGTWFSGFVVDNYKLDEGHNWMKVWFVPAYIALGVLLYFILFFREK
                     RAAAAIST"
     gene            2502..3158
                     /locus_tag="VGM24_01140"
     CDS             2502..3158
                     /locus_tag="VGM24_01140"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY4153991.1"
                     /translation="MRVLSDSYRHKGLRKQLVALIRSKGITEERVLAAIQEIPRHFFM
                     DSAFDQIAYEDRAFPIAEGQTISQPYTVAYQTQLLEVRPHEKVLEVGTGSTYQACVLA
                     EMDAQVYTIERQKNIFEQNKLFNYPRKYPSIRYFYGDGYEGLPTFAPFHKIIVTAAAP
                     EIPQKLLDQLMPGGKMVIPVGDGKVQRMIRINKYPDGSVGEEAFDDFSFVPMLQGKNN
                     "
     gene            3166..3756
                     /locus_tag="VGM24_01145"
     CDS             3166..3756
                     /locus_tag="VGM24_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4153992.1"
                     /translation="MTPERHQRLTQVLDKRQNDLTVVLENVFDPHNISAVMRTCDAVG
                     IQEINVLNTRIGPHKKWGAKSSSSAAGWLTINQYSDPADCFARLRKQYDLILTTHLSA
                     NAISLYEIDFTRPVALIFGNEHDGVSEEIRQMADGNFIIPQVGIIRSLNISVACAVTL
                     YEAFRQKKAAGHYEQVKLRKEEYERIQHEWGFIADP"
BASE COUNT         1031 a         1033 c          934 g          933 t
ORIGIN      
        1 ttttgacgat cactttcttt gctccgtagt gggtcatttc ttcttttacc aggtaatggt
       61 ctgcgtcata ttcgaccagg tggctgtctt tgcttacgcc ggtccggcct ctccagatat
      121 ccaattgaac cggttcccac aattttgttt ttgccgattc gtatgcggca tccagcaccg
      181 cattcacgat atacccatcg taaaaagttt ctttcggagc gctccctttt tccatcgcat
      241 tgaacatatc ggcaaacata tggttatagc cgaggtcgtt gagttcatct cccaccggaa
      301 ataaccagcc cgtattggac tccgcttttt cagccacata atcggcgcct tttccggtcg
      361 tgaacatttc cagtccggtc cggagaaaat tattgatcca gatggtgcct tcggttccca
      421 tcacttcatc ccgcaggtcc aggccaccgc gaaaggtcca gctgacctca aactggccga
      481 tcgctccgtt ttcgtatttc accaggccga tggcatggtc ttccgcttcg atgggtttca
      541 cctgcgtgtc cgcccagcac attacttcca ccggtttgat atctttcccg ataaaattcc
      601 ttccgatctc cacgcaatgg caacccagat cgaggatgca gccgccacct gcctgctcca
      661 ggtcccaaaa ccagtcgctg tgcgggccgg gatgggtttc ccttgacttg gaccacagga
      721 ttcttcccag ggccccgttc ttcacacttt cgtaggcttt taaaaatttg ggggtataca
      781 ccaggtcttc caggtagcca ttgaatatcc ccgctttctc caccgcttcc agcatgcgct
      841 ttgcttcggc agccgtacgt ccgagcggtt tggtggtaat gaccgctttc ttgtgtttgc
      901 agcaaagcaa aaccgccgct tcatgcaggt tattcggaag cgaaatgcaa accacatccg
      961 tttccggatg ggcaatcgcc tcctccatat tgatcgtcca gtgagaaact ccgtaatccg
     1021 aagcaaattt ctttgcgctt tcctcgcggc gggaatacac ggtcacgatg cggtcgcggc
     1081 ttcgctgccc ctgtagtgaa tcggcataaa acctcccgat aaatccggaa cctaacatgg
     1141 caattctcgt catgattatt tatttaaatt tggattaaat gtttactggc gacggatgag
     1201 ccggatgaac taagtgctga tggcagccgc ggcccttttc tcccggaaaa ataaaataaa
     1261 atagagcaat acgcccagcg caatgtaggc aggtacaaac caaaccttca tccagttgtg
     1321 gccttcgtcc aatttataat tatccaccac aaatcccgag aaccaggttc cgatgaacat
     1381 acccacgcca taggtggcaa aggtaaataa tccctgcgca gcatttttga taccggcgcc
     1441 tgcttttttc tcggtataca tatatccggt aacaaaaaag aaatcataac agaccccatg
     1501 cagaaggata ccggcataca gcatccagag attgctgctt aaatcgccat aggcaaaaca
     1561 aacataccgt aaaatccagg ccgtcatccc cataagcaac atattcttta ccccgatccg
     1621 gttaaacagg aaaggaatgg ccagaataaa tacgccttcc gagatctggc ccagaatcat
     1681 ttttgaagcc gcattatgaa ggcctatttc attcaaaaaa gcgttggcaa agccgtaata
     1741 gaaagaaagc ggtatgcaaa ccaggaccgc tgcaataata aagatcaggt aggctttatt
     1801 tttaaaaaga acaaacgcat cggttccgag ggccttagcc gcggagacct ccgtggatct
     1861 ggctttgggc ggggtattgg gaagaatgaa actgatcagc ccgatcccgg cggatgcagc
     1921 cgctgccagg taaaacgtat tggctgtttt ttcccattgc agattgccga tcagcaaacc
     1981 cgcaacaatc caacccaggg ttccaaaaac ccgtatccag ggaaactggc ggcccgggtc
     2041 actcatctgg taaaaagcaa tgctgttgga aagcgcgatc gtcggcatat acaaaaggga
     2101 ataaaacaga atgatccagt aaaaaggagc attcaccgta acgctggaag ccagaaagag
     2161 cagtacggcg ccgatcaggt gcaggacgcc catgatccgt tgcgcggcaa aataccggtc
     2221 ggcaatcagg ccgataaaaa aaggagagat catggtggcg atggccagcg cgctgtacgc
     2281 cgccccgatc tgaacgccgg tggagtgcag gttagcaccc atgtaggtgc tcatggtcac
     2341 ataccaggcc ccccagacaa aatattcgag aaacatcatc aggcagagca gaaacgtggt
     2401 gactggcttc attaatatgc atttttaggg agggcttctc ccgatgaaaa gttacaaatg
     2461 aaaaagactc attgtaaata attcttcaat tatcttgcag tatgcgcgtg ttatcggact
     2521 catatcgcca caagggcctg agaaagcaac tggtggccct gatcagaagt aaaggcatca
     2581 cggaggaaag agtgctggcc gccatccagg agatcccgcg ccatttcttt atggattccg
     2641 cctttgatca gattgcctat gaggaccggg cctttcctat cgccgagggc cagacgattt
     2701 cccagcccta taccgtggcc taccagaccc agttgctgga agtcaggccc catgaaaaag
     2761 tgctggaagt cggaaccgga agcacttacc aggcttgcgt gctggcggaa atggatgccc
     2821 aggtatatac cattgaacgg caaaaaaaca ttttcgaaca aaataaattg tttaactatc
     2881 cccggaaata cccgtccatc cgttattttt atggagatgg ctatgaaggc ctgcctacat
     2941 ttgccccctt ccacaaaata atcgtcacgg ccgctgcgcc ggaaattccg cagaaattac
     3001 tggatcagtt gatgccgggc ggaaaaatgg tgatcccggt cggagatggg aaagtgcagc
     3061 ggatgatccg catcaataaa tatccggacg gttccgtcgg ggaagaagct tttgatgatt
     3121 tttcatttgt cccgatgctc cagggaaaaa ataattaagc tgttcatgac gccggaaaga
     3181 catcaacgac tgacccaggt tttggataaa aggcaaaacg acctgaccgt ggtgctggaa
     3241 aatgtgtttg acccgcataa tatttctgcc gtaatgcgga cctgcgatgc ggtggggatc
     3301 caggaaatca atgtactgaa tacccgcatc gggccgcata aaaaatgggg ggcaaagagc
     3361 agcagcagcg cagccggatg gctgaccatt aaccagtatt ccgatccggc ggactgcttc
     3421 gccaggctgc gcaaacaata tgatcttatt ttaacgaccc atctctccgc aaacgccatt
     3481 tccctttacg aaattgattt caccaggccg gttgccctca tttttgggaa tgaacacgac
     3541 ggggtgagcg aagaaatccg gcaaatggcc gatggcaatt tcattatccc ccaggtcggt
     3601 atcatccgtt ccttaaatat ctccgtggcc tgcgccgtca ccctctatga agccttccgc
     3661 cagaaaaaag cggccggtca ttatgaacag gtaaaattgc ggaaagagga atacgagcgt
     3721 attcagcacg agtggggatt cattgctgac ccctgatcca ttaaccgaat tcattatgca
     3781 aacgaaaatc tacgggccgg gtattgttat tttgaccgcc ggtatcgtcg ccggcacggc
     3841 cgacgccctg gccgctattc ttttttatac gccatccatc agtctgaaca acagcatccg
     3901 gatttttcaa ggcattgcga gcggtctgtt c
//
LOCUS       DASYCU010000056         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4508638, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000056 DASYCU010000000
VERSION     DASYCU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4508638"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..402)
                     /gene="trxA"
                     /locus_tag="VGM24_01150"
     CDS             complement(85..402)
                     /gene="trxA"
                     /locus_tag="VGM24_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY4153993.1"
                     /translation="MATEFTDANFQEKVIASDKLTVIDFWAEWCGPCRAIGPVIEELS
                     KEYDGKINIGKVNVDHNPELSVNYGITSIPAILFIKGGKVVDKLVGAQPKSNFVKKIE
                     SHI"
     gene            complement(522..>2318)
                     /gene="dnaE"
                     /locus_tag="VGM24_01155"
     CDS             complement(522..>2318)
                     /gene="dnaE"
                     /locus_tag="VGM24_01155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY4153994.1"
                     /translation="KTALELIVQNHGIAIDIDQIPLDDKKTFELYQHGETIGTFQFES
                     PGMQKYLRDLKPDKFDDLTAMNALYRPGPMAYIPAFIDRKQGREPIRFDLPEMEEYLK
                     DTYGITVYQEQVMLLAQKLAGFSKGDADVLRKAMGKKQKSILDKMKNQFISGAAKKGH
                     DAVVLEKIWSDWEAFAQYAFNKSHSTCYAYVAYQTAYLKSHYPSEYMAAVLNHCGSIE
                     KITFFMEECKRMGLKVLGPDVNESKQGFAVNSKGEIRVGLGGLKGVGEAAVGAIIEER
                     EKGTPFISIFDLIRRINQRTVNKKTLESLAYAGAFDCFPELHRAQYFFVAPGDTSTGL
                     EKIIRFGNVVQAQNVQHSNTLFGDSGMDFDVQPPKIPNCEPWTLTELLNHEKDVTGMF
                     MSGHPLDHFKFEISHYGIIHLADFNEIREAVTLQSNPGKNYRLAGLVTDAQHRVTKTG
                     KQFGSFTLEDYSGKSEFILWSEDYARFSNYLEKGKNIFLTGYFKARYNREEFEFKVDK
                     MMMLESIKPVMTRQLVLDVEARHLSEPMVRFMEKNVRKHPGKSSLKFNILESRTNQRI
                     SMYSMDNGFEMNDEMAVFLQNSPEFGVQIVYS"
BASE COUNT          521 a          604 c          534 g          659 t
ORIGIN      
        1 aaaaatagcg ggtaaattcc taaaattctg ctaaataaaa aagcgcccca ttccggcggc
       61 gctgtgattt tattttttac tttttcagat atgcgactcg atcttcttca caaaattgct
      121 cttgggctgc gcaccaacca gtttgtcgac gaccttcccg cccttgatga aaaggatggc
      181 cgggatggaa gtaatgccat agttcacaga caattccgga ttgtgatcca catttacttt
      241 tcctatgttg atcttgccgt catattcttt tgaaagttct tcgatgaccg gaccgattgc
      301 gcggcaggga ccacaccatt cagcccagaa atcgatcacg gttaatttat cagaagctat
      361 caccttttcc tgaaagttcg cgtcggtgaa ttcagttgcc atagtattcg ttttaatttt
      421 aggtaaaatt cagtttattt atttataatt tttgtcgttt caacgacaga gatgcaaaaa
      481 tgtttccact cccaaaatcc cttcattctg tcagcttccg gtcaggaata tacgatctga
      541 acgccgaact ccgggctgtt ttgtaaaaag acggccattt cgtcattcat ttcaaaacca
      601 ttgtccatac tgtacatgct gatccgctgg ttggtcctgg attccaggat attgaatttc
      661 agggaggatt tgcccgggtg cttccggacg ttcttttcca taaagcggac catcggttcg
      721 ctgaggtgcc gcgcctccac gtcaagcacg agctgccggg tcatcaccgg cttaatgctc
      781 tccagcatca tcattttatc cactttgaac tcgaactcct cgcggttata cctcgctttg
      841 aaataaccgg tcaggaagat gttctttcct ttttccaggt aattcgaaaa ccgtgcatag
      901 tcttcgctcc ataaaatgaa ttcgcttttt ccggaataat cttccagcgt aaaagatccg
      961 aactgctttc ctgttttggt cacccggtgt tgggcatccg tgacgagccc ggccagccgg
     1021 taattttttc ccggattgga ttgcagagtc accgcttccc tgatctcatt gaaatccgcc
     1081 aggtgaatga tcccgtaatg actgatctca aacttgaaat gatccagcgg atgcccgctc
     1141 atgaacattc ccgtaacgtc tttttcgtga ttgagcaatt cggtcagggt ccagggttca
     1201 caattgggaa tcttgggcgg ctgcacgtcg aaatccatgc ccgagtcgcc gaaaagggta
     1261 ttgctgtgct gaacgttttg ggcctgcacc acattcccga agcggatgat cttttccagc
     1321 ccggtgctgg tatctcccgg agcgacaaaa aaatactggg cgcggtgcag ttccgggaaa
     1381 caatcgaaag cgccggcgta ggccaggctt tccagcgtct ttttatttac ggtgcgctga
     1441 ttgatccgcc tgatcaggtc gaaaatggaa atgaagggag tgcctttttc tctctcttcg
     1501 atgatggcgc ccacggccgc ttcacccacg ccttttaatc cgccgagtcc cacccggatc
     1561 tctcccttac tgtttacggc gaagccctgt ttggattcgt tcacatccgg tccgagcact
     1621 ttcagtccca tccgtttgca ttcctccatg aagaaagtga tcttttcaat gctgccgcaa
     1681 tggttgagca cggcggccat gtattcggaa gggtagtggg atttcagata agccgtctgg
     1741 taagctacgt aagcataaca ggtggaatgc gatttattaa aggcatactg cgcaaaagct
     1801 tcccagtcgg accagatctt ttcgagcacc accgcatcat gccctttttt cgcagcgccg
     1861 gagatgaact ggttcttcat tttatccagg atcgatttct gcttcttacc catcgccttt
     1921 cgcagaacat ccgcatcccc cttgctgaac ccggccagtt tttgcgccag cagcatcacc
     1981 tgctcctggt atacggtgat cccatacgta tccttcaggt attcctccat ttcgggaaga
     2041 tcaaagcgga tgggctcccg gccctgcttg cggtcaatga aggcagggat ataggccatc
     2101 ggccccggac gatatagggc attcatcgcc gtcaggtcgt cgaatttatc gggtttcaga
     2161 tcgcgcaggt atttttgcat acccggactt tcaaactgga aagtgccaat ggtttccccg
     2221 tgctggtaaa gctcgaaggt ctttttatca tccagcggga tctggtcgat atctatggcg
     2281 atcccgtgat tttgaacaat cagttccagc gcggtctt
//
LOCUS       DASYCU010000057         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4943122, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000057 DASYCU010000000
VERSION     DASYCU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4943122"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..3270
                     /locus_tag="VGM24_01160"
     CDS             133..3270
                     /locus_tag="VGM24_01160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY4153995.1"
                     /translation="MNQNEVFRKLYEKLNPQQKLAVDTLEGPVMVIAGPGTGKTQILG
                     ARIGKILLETDTDPGNILCLTYTDAGAIAMRRRLLSFIGPDAYKVNIYTFHAFCNDII
                     QDNLSYFERTSLDPVSVLEQVQYLKQLIDGFAKNHPLKRFRSDAYYEVGNLRALFSSM
                     KKEGWTPGFIGKKIDEYLEDIQTREKYIYKKSGKGFSKGDKKQADLDEEGIRMEKLRS
                     AANEFERFQLLMRNKSRYDFDDMINWVIRAFSENEQLLRRYQEKYLYILVDEYQDTSG
                     TQNKLVELLISFWDIPNVFVVGDDDQSIYRFQGANMDNMLHFANRYKKDLLTIVLTNN
                     YRSTQPILDVSKALISRNAERLVNKIEGLSKELLSSNETIRHLTGLPVIREYDTERNE
                     MIDIVMQVEQLISSGVPPGRIGIIYKENRYGEELLRFFKLKNLPVYSRRSVNLLKDPL
                     IKKILLLISYLATEQEIPFSGDEMLFEILHADWFHIPAIEIASLSSEVAQKQYTKEKT
                     SLRKWLSDKSRQPARDLFSAPVNERLAGAGRVLESLISKVSNNTLQSLFECIFRETGL
                     LNEIMNSHEKPELLQILTAFFDFIKEETRRTPSMNLKELVTHLELMDKEDIPLPLVEV
                     SGRTDSVNLMTVHGSKGLEFDQVFIAGSNAHYWEKKRKPSTTFHYPDTLFITKDNEED
                     HEELRRLFYVALTRAETHLFISYSRFRNDGKEAEPSVFIEELRAGFDLETEKPVPSRQ
                     AVSGFQLLLLQGEHAPEIERTELDFATRLLDNFQMNVTALNNFLNCPLEFYYKNLLRI
                     PSPKNENTEFGSAVHYSLEQLFRNMQENNNEFAGIPFFLNAFDWYMNRHRESFTSEQF
                     ARRMDYGHIVLPEYYTKYVHSWNKIVSIERTVRNVAIQGVPLKGKIDKLEFEGKLVNV
                     VDYKTGNPENGKKKLARPSEKDPNGGDYWRQAVFYKLLLDQQAQRDWQVLSVEFDFIE
                     PDNKKVYRREKLIVSPEDTDTVTRQITGTWRKIQNHEFYTGCGKPDCHWCNFVKTNRL
                     DIAEAAGEEEEHASL"
     gene            3329..4744
                     /locus_tag="VGM24_01165"
     CDS             3329..4744
                     /locus_tag="VGM24_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HEY4153996.1"
                     /translation="MKTFLSLLGIGIILLRFPGFTGCANIIPPTGGPRDSIPPVLVSA
                     EPDENSLHFKSNKIVLTFDEFIDLKDVRQNLIVSPVPKIQPNVEFKLKTLTIRLKDTL
                     RPNTTYSLNFGKAVVDNHEGNVLKNFTYVFSTGDYIDSLKYSGRVVMAYTGKPDSTLT
                     VILHDKLDDSAVVKERPRYITRLDSSGNYTFYNLKPGTYALYALKDESGSHTYTTKSQ
                     IFAFADSPVQLKENMTAPELYAYADTSGEKPAKKLLLAPPVKPKKEDKDKPKRLSVSG
                     NIPGGLLDLHDQFEFIFVVPIRNFDSTKVHFSKDSFETISAHHFVEDSLHKKVTLVYQ
                     WTPDTRYQLILEKDFAEDTLGEKLLKTDTISFATKKESDYGNLRLRFKNLDLSRHPVL
                     LFIQSDKIMQQYVFGRSVRYDNKLFEPGDYELRILYDTNQNGVWDPGNFFEHRQPEKV
                     QAIRRKLSVKANWDNEVDITL"
     gene            4755..>5068
                     /locus_tag="VGM24_01170"
     CDS             4755..>5068
                     /locus_tag="VGM24_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEY4153997.1"
                     /translation="MQFSSVLLETAVNEFAKLPGIGRKTALRLVLHLLKQDPAAARGF
                     GEILIKMRQEIRFCQRCHNVADADICSICANSMRRQDMICVVETVRDVIAIESTQQFS
                     GT"
BASE COUNT         1387 a         1314 c         1237 g         1130 t
ORIGIN      
        1 cactttgaac gactgggggt tcttcatatt tcggattata tcggattgta cggatctata
       61 acgataaaac ggcgggaata atattccgga tttaagaaag tttccggatt ttgaataact
      121 tttcaatcat atatgaatca gaacgaggtc tttagaaaac tgtacgaaaa actcaatccc
      181 cagcagaagc tggccgtgga taccctggag ggcccggtta tggtgatcgc cggcccggga
      241 acgggtaaaa cccagatcct gggcgcccgg atcgggaaaa tcctgctgga aacggacaca
      301 gatcccggga acatcctttg cctcacctat acggacgccg gggccatcgc catgcgccgc
      361 cgcctcctgt cttttatcgg tccggacgcc tacaaggtga atatctatac ttttcacgcg
      421 ttctgcaacg atatcatcca ggacaacctg tcctatttcg aaaggaccag cctggacccg
      481 gtctccgttc tggaacaggt acaatacctg aaacagctga tcgatggctt cgcgaaaaat
      541 catccgctca agaggttccg gagcgacgcg tattatgaag tgggcaacct gcgggctctt
      601 ttcagcagta tgaaaaaaga aggctggaca cccggtttca tcgggaagaa gatcgatgaa
      661 tacctggagg acattcaaac ccgggaaaaa tacatctaca agaaaagcgg aaaagggttt
      721 agtaagggcg acaaaaaaca ggcggatctg gacgaggaag gaatacgcat ggaaaaactg
      781 cgctccgccg ccaatgaatt tgaacggttc cagctgctga tgcgcaacaa aagccgctac
      841 gatttcgatg atatgatcaa ttgggtgatc cgggcttttt ctgaaaacga acaactgctg
      901 cgccgctacc aggaaaaata cctctacatc ctcgtggatg aataccagga taccagcgga
      961 acccagaaca agctggtgga attgctgatc agtttctggg atattcccaa tgtgttcgtg
     1021 gtgggagacg atgatcagag catctaccgg ttccagggcg ccaatatgga caatatgctc
     1081 cattttgcaa accggtataa aaaggatctg ctcaccattg tgctcaccaa caattaccgc
     1141 tccacccagc cgatcctgga tgtttcaaaa gccctgatca gccggaatgc ggaacggctg
     1201 gtaaataaga ttgaagggct tagcaaggaa ctcctttctt ccaatgagac catccgccac
     1261 ctgaccggcc tgccggtcat ccgcgaatac gatacggaac gcaacgaaat gatcgatatc
     1321 gttatgcagg tggaacagct gatcagctcg ggtgtccctc ccggccggat cggcattatc
     1381 tataaagaaa accggtacgg tgaggagttg ctccggttct tcaaactgaa aaacctcccg
     1441 gtttacagca ggcgaagcgt gaacctcctg aaagatcccc tgattaaaaa aatcctgctg
     1501 ctgatctcct acctggccac ggaacaggag attccgttca gcggggatga aatgcttttt
     1561 gagatcctgc acgcggattg gtttcatatt ccggccatcg aaattgcaag cctcagctcg
     1621 gaggttgccc agaaacagta taccaaagaa aaaacttccc tgagaaaatg gcttagcgat
     1681 aaaagcaggc agccggcccg ggatcttttt tcagcacccg tgaatgagcg cctggcaggc
     1741 gccggccgtg ttttggaatc cctgatcagc aaggtgtcca acaacaccct gcagtccctg
     1801 ttcgaatgta ttttccgcga aaccggtctg ctgaatgaaa tcatgaacag tcacgaaaaa
     1861 ccggaattgc ttcagatcct caccgcattt ttcgatttca taaaagagga aacgcgcaga
     1921 acgccttcca tgaacctgaa ggaactggtg acccatctcg aactgatgga caaggaagac
     1981 atcccccttc cgctggtgga agtgagcgga cgcaccgatt ccgtcaacct gatgacggtg
     2041 cacggttcca agggacttga attcgaccag gtgttcattg ccggctccaa tgcccattac
     2101 tgggagaaaa agagaaagcc gtccactacc tttcattatc ccgacactct ctttattaca
     2161 aaggacaatg aagaagacca tgaagagctg agaaggcttt tttatgtagc gctcacccgc
     2221 gccgaaaccc atctttttat ttcttacagc cggttccgaa acgatgggaa agaggccgag
     2281 ccttccgttt tcatcgaaga actccgggcc gggttcgacc tcgaaacgga aaaaccggtc
     2341 ccttcccggc aagccgtttc cggtttccag ctcctcctcc tccagggcga acacgctccg
     2401 gaaatagaac ggaccgaatt ggatttcgca acccggctgc tggacaattt ccagatgaat
     2461 gttaccgcac tcaataattt cctgaattgt ccgctggaat tctattataa aaacctgctc
     2521 cggatccctt ccccgaaaaa tgaaaacacg gaattcggtt ctgccgtcca ttattcgctt
     2581 gaacaattgt tccggaacat gcaggagaac aataatgagt ttgccggcat ccctttcttc
     2641 ttaaacgcgt tcgactggta catgaaccgg caccgcgaaa gttttacttc ggaacaattt
     2701 gcgcggcgaa tggattacgg ccatattgtt ttgccggaat actatactaa atatgttcat
     2761 tcctggaata agatcgtgag cattgaaaga acggtgcgca acgtggccat ccagggcgtt
     2821 ccgctgaaag gcaagatcga taagctggag tttgaaggaa aactggtgaa tgtggtcgac
     2881 tacaaaacgg gtaatccgga aaacgggaag aaaaaactgg cccggccttc agagaaagac
     2941 cccaatggcg gggactactg gcggcaggcc gttttctata aattgctgct ggatcagcag
     3001 gcccagcgcg actggcaggt gctgagcgtc gaatttgatt ttattgaacc ggataataaa
     3061 aaagtctatc gccgggaaaa attgatcgtc agcccggagg acacggacac cgttacccgg
     3121 cagatcaccg ggacctggcg gaaaatccaa aatcacgagt tttacaccgg ttgcggcaaa
     3181 ccggattgcc actggtgcaa ctttgtaaag accaaccggc tggatattgc agaagcagcc
     3241 ggggaggagg aagaacacgc ttccttataa aatcataaat ctgtcaaaat cccatcgggg
     3301 tcaactaagt ttgcaggcga caaagtatat gaagaccttt ctttctttac tcgggattgg
     3361 cattatttta ctccggttcc cgggttttac gggttgcgca aatatcattc cgcccaccgg
     3421 cggcccccgg gatagcattc cccccgtgct ggtgtctgcc gaacccgatg aaaattcact
     3481 tcacttcaag agcaacaaga tcgtactcac cttcgatgag ttcatcgatc tcaaggacgt
     3541 gcgccagaac ctgatcgtat cccccgttcc gaagatccag cccaacgttg agtttaaact
     3601 caaaaccctt accatccgct tgaaggatac cctccggccc aacactacct actcgctgaa
     3661 tttcggcaag gcggtggtgg acaatcatga agggaacgtg ctgaaaaatt tcacctatgt
     3721 tttttcaacg ggagactata tcgactccct caaatacagc ggcagggtag tgatggccta
     3781 tacgggcaag ccggacagca ccctcaccgt gatcctgcac gataaactcg atgattctgc
     3841 ggtggttaaa gaaaggcccc ggtatatcac ccggctggac agttccggaa actatacttt
     3901 ctataatctg aagcccggca cctacgcgct gtatgcgctg aaagatgaat ccggatcaca
     3961 tacctacacc accaaatcac agatttttgc ctttgcagac agcccggtgc agcttaaaga
     4021 aaacatgacc gcccccgaac tctatgcgta cgccgatacc agtggcgaaa agccagctaa
     4081 aaaactcctc ctcgctccgc cggtaaaacc aaaaaaagaa gacaaggaca aacccaagcg
     4141 gctttccgtc tccggaaata ttccgggagg tcttctggac ctgcacgatc agtttgaatt
     4201 tattttcgtg gtgccgatcc ggaatttcga ttctaccaaa gtccatttca gcaaggacag
     4261 ttttgagact atcagcgcgc accatttcgt ggaagacagc ctgcataaaa aagtgaccct
     4321 ggtctaccaa tggaccccgg acaccaggta ccagctgatc ctggaaaagg attttgcgga
     4381 ggataccctg ggcgaaaaat tactgaaaac ggataccatc agcttcgcta ccaaaaagga
     4441 atcggactat ggaaatctgc gcctgcggtt taaaaatctg gacctctccc ggcatccggt
     4501 gcttctgttc atccagagcg ataagataat gcagcagtat gtttttggca ggtccgtccg
     4561 gtacgataat aaactctttg aacccggcga ttatgagctt cgcatactct acgataccaa
     4621 tcagaacggg gtctgggacc ccggtaattt ctttgagcac cggcaaccgg aaaaggtcca
     4681 ggccatccgg cgaaagctct cggtaaaggc gaactgggac aacgaagtgg atattacttt
     4741 gtaattttgc tcccatgcaa ttttcttcgg tcctgctaga aacggcggtc aatgaattcg
     4801 ccaaattacc cggcatcgga aggaaaacgg ccctccggct ggtactccat ttactcaagc
     4861 aggatcctgc cgcagcaagg ggattcgggg agatcctgat caaaatgcgg caggagatcc
     4921 gattctgcca gcgctgccac aatgtggccg atgcggatat ctgctccatt tgcgcgaatt
     4981 ccatgcgccg ccaggatatg atctgcgtgg tcgaaaccgt ccgggatgtg atcgccattg
     5041 aaagcaccca gcaattcagc ggtactta
//
LOCUS       DASYCU010000058         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_304952, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000058 DASYCU010000000
VERSION     DASYCU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304952"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /gene="manA"
                     /locus_tag="VGM24_01175"
     CDS             complement(<1..807)
                     /gene="manA"
                     /locus_tag="VGM24_01175"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEY4153998.1"
                     /translation="MERSKDKLMPIRGKIQHYAWGGDFYLPKLLNLPVKKGETYAEYW
                     LGTHSRGSAELTDKKGGRILLRDYIRAYPKETLGEKTAAQYGELPYLLKILDVKEMLS
                     IQVHPSKANARKEFDAENQKGIDLSAPERNYKDDNHKPELMLALSEFWLLHGFKPVKQ
                     LERILHEIPELHFLVPVFGNGNYKALYRQVMEMPQAQVNRHLKTLLDRIVPHYQVNKL
                     KKNKEDFWAARAALTYQHKGDIDRGIFSIYFFNLVQVLPGHAVFQDAGVPH"
     gene            840..1199
                     /locus_tag="VGM24_01180"
     CDS             840..1199
                     /locus_tag="VGM24_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY4153999.1"
                     /translation="MAAHNEFGKAGEEMAAGWLCRKGFHLLAMNWKSARFEIDIIASR
                     GGVLHFIEVKTRHDDRFGLPEDWVGKKKLGHLLKAGVAYQEKYPEWNQVQYDILSILV
                     DQTGKPDFFFIEDVYCW"
     gene            1199..>1901
                     /locus_tag="VGM24_01185"
     CDS             1199..>1901
                     /locus_tag="VGM24_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154000.1"
                     /translation="MDTRNRQRSEMIPFYQENIQRQQDLIRQKKKIKAGLAAARLAVF
                     LFAAGLVYYFWPSVGSACLSAGVCGIVLVVLIFTDAGKTGEIRNHERLIRINRHETDA
                     LQQQLNEYDDGRVFANPLHAYASDLDLFGPASLFQFISRCHADQSKKLLADQLLNPLP
                     VQTIGEKQAAAKEVSDKVGWSQQFQSAAMANPLTFQTEKRMKQWMQEQPGIFEKGYWK
                     GIVILYSLISLSLLTL"
BASE COUNT          464 a          467 c          472 g          498 t
ORIGIN      
        1 atgcggcacg ccggcatcct gaaaaacagc atgacccggc agcacctgca ccagattgaa
       61 gaaataaatc gaaaaaattc cccggtcaat atctccctta tgctggtagg taagggcggc
      121 ccgggcggcc cagaaatctt ccttgttttt tttcaacttg ttcacctggt aatgcggaac
      181 gatccggtcc agtaaagttt tcagatgccg gttcacctgg gcctgcggca tttccatcac
      241 ctgccggtaa agggccttgt aatttccgtt cccgaaaacg ggaaccagaa aatgcagttc
      301 cggaatttcg tgcaggatgc gctccagttg ctttaccggc ttaaagccat gcaataacca
      361 gaattcgctt agggccagca tgagttccgg tttatgatta tcgtctttat aatttctttc
      421 gggagccgaa agatcaatcc ccttctgatt ttctgcgtca aactccttcc gggcgtttgc
      481 ctttgacgga tgcacctgga tggagagcat ctccttcaca tccagaattt tcagcagata
      541 gggcagttcc ccgtactgag ccgctgtttt ttcgccgagg gtctctttgg gataggcccg
      601 gatataatcc cggagcagga tcctgccccc ttttttgtcc gttaattcag cacttcccct
      661 gctatgcgtt ccaagccaat attccgcgta tgtttcaccc ttttttacgg gtaaattcag
      721 caatttcgga agataaaaat ccccgcccca tgcgtagtgt tgtattttcc cccggatagg
      781 catcagctta tctttactcc gttccatatt ttttattagt ggctcaaagt aaagcaaaaa
      841 tggccgcaca taacgaattt ggaaaagcgg gtgaagaaat ggctgccgga tggctttgtc
      901 ggaaaggttt tcatctgctg gccatgaact ggaaatccgc ccggtttgaa atcgatatca
      961 tagcttcccg gggtggagtc ttgcatttta tcgaggtcaa gacaaggcac gatgaccggt
     1021 ttggcctccc ggaagactgg gtggggaaga aaaaactggg ccatttgtta aaagcgggcg
     1081 tggcttatca ggaaaagtat ccggaatgga accaggtcca gtacgatatc ctttccattt
     1141 tagttgatca gaccggcaaa ccggatttct tttttataga agatgtatat tgctggtaat
     1201 ggatacccga aataggcagc gttccgagat gattccattt tatcaggaga atattcagcg
     1261 gcagcaggac ctgatccgtc agaaaaagaa aataaaagcg ggtttggctg cagcgagact
     1321 ggccgttttc ctgttcgccg ccgggctggt ttattatttc tggccttctg tgggttcggc
     1381 ctgcctgtcc gccggagtct gcgggatcgt tctggtcgtc cttatattca ccgatgccgg
     1441 gaaaaccggc gaaatcagga atcatgaaag gctgatccgg attaaccgcc acgaaacaga
     1501 tgcattacag cagcagctca atgaatatga cgatggacgg gtattcgcca atccgcttca
     1561 cgcctatgca tcggatctgg atttgttcgg acccgcttcg ctttttcaat tcatcagccg
     1621 ctgtcatgcg gaccagtcca aaaaattact ggctgatcaa ttgttaaacc cgcttccggt
     1681 ccaaactatc ggcgaaaaac aggctgccgc aaaagaggtt tcggataagg tgggctggtc
     1741 acagcaattt caatcggcgg cgatggccaa cccgctgact ttccaaaccg aaaagcggat
     1801 gaagcaatgg atgcaggaac agccaggcat ttttgaaaag ggatactgga agggaatcgt
     1861 tatcctgtac tcgctgatca gcctttccct gctcacgctg t
//
LOCUS       DASYCU010000059         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4220108, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000059 DASYCU010000000
VERSION     DASYCU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4220108"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VGM24_01190"
     CDS             <1..1437
                     /locus_tag="VGM24_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4154001.1"
                     /translation="DKDAWKLTGGANNWMGMIVDSKTGIAYIPIGSTSMDFYGGKRKG
                     ADLYGDCLLALDASTGKLKWHFQYIHHDTWDWDPPSAPVLLTVNHDGKSIDAVAQTTK
                     TGFVFLFNRENGNPLFPIVETPVDTVTELTGEKLWPTQPVPQKPLPFVRQSFSEKDIN
                     PYLSPEEYADVKQQLSTYSTGMFKPQSKAGTVIFPGFDGGSEWGGPAVDPRTGILYVN
                     ANQMTWILKMLDIQNKPQSKENFGEAGQRLFKQNCMSCHGPDRKGGGNYPSILDVNKK
                     YDKNNFISFISAGRRMMPAFQFLKPEEKEAIASFILENKKEQVKPFVLELTAEDKFRQ
                     VPYSISGYNRFVSKSGQPAIAPPWGTLNAIDLNSGDLKWSVVLGEEDSLKARGIPQTG
                     TENYGGPVVTAGGIIFIAATKDAKLRAFNKENGKLLWQADLPAAGFATPATYEIDGRQ
                     YVAIACGGGKLNKISADAYVVFALPEKK"
BASE COUNT          461 a          329 c          396 g          354 t
ORIGIN      
        1 gacaaagatg cctggaaatt gaccggcggg gcaaataact ggatgggcat gattgtcgat
       61 tcaaaaacag gtatcgccta tattcccatc ggatcgacgt ccatggattt ctatggcggg
      121 aaaagaaagg gtgcggattt atatggagat tgtctccttg ccctggatgc atcaaccgga
      181 aaattaaaat ggcatttcca atatattcat catgatacct gggactggga tccgccttcg
      241 gcccctgtat tgctgaccgt gaaccacgat ggaaaatcca ttgacgcggt tgcacagaca
      301 accaagacag gatttgtatt cctctttaac cgggaaaacg gaaatccgct ttttccgatt
      361 gtggaaaccc ccgtcgatac ggtcaccgaa ttgactggcg agaaactctg gcctacgcaa
      421 ccggtaccac aaaaacccct gccgttcgtg cggcaatctt tttccgaaaa agacattaat
      481 ccctatttgt ctccggaaga gtatgcagat gtaaaacagc aattgtccac ctacagcacc
      541 ggcatgttta aacctcaatc caaagcggga actgtgatct ttcccggatt tgacggagga
      601 tcagaatggg gagggcccgc cgttgacccg cggaccggca tattgtatgt gaatgcaaat
      661 cagatgacct ggatactgaa aatgctggat atccagaata agccgcagag taaagagaat
      721 ttcggagaag caggacaacg cttattcaaa caaaattgta tgtcctgcca tggccccgac
      781 cgaaaagggg gtgggaacta tccctccatt ctggatgtga acaaaaaata cgataaaaac
      841 aattttatca gttttatcag tgcaggaaga agaatgatgc ctgcatttca gtttctgaaa
      901 ccggaggaaa aagaagccat tgcgtctttt attctcgaaa ataaaaaaga acaggttaaa
      961 ccctttgtac tggagttgac ggcagaggat aagttcaggc aggtgccgta tagcatttcc
     1021 ggatacaaca ggttcgtttc aaagtccggc cagccggcca tcgcgccgcc ctggggaaca
     1081 ttaaatgcga ttgacctgaa cagcggggac ctgaaatgga gcgttgtgct gggcgaggaa
     1141 gacagtctga aagcaagagg gattccgcaa accgggacgg aaaattatgg cggccccgtg
     1201 gttactgccg gtgggatcat atttattgca gcaacaaaag atgcaaaact gagggcgttc
     1261 aataaagaga acggtaaact gttgtggcag gcggatttac ccgctgcagg atttgctacg
     1321 ccggcaacct atgaaatcga cggacgtcaa tatgtggcca ttgcctgcgg gggtggaaaa
     1381 ttaaataaga tctctgctga tgcctatgtg gttttcgcgc tgcctgaaaa gaaataaaat
     1441 gcagaaaggc agagtgcaaa aacaggacgg gagcaaataa aaattcgggt tatgaaatta
     1501 tttatattaa aatggacagg cgctgtgctg tttgcaattt
//
LOCUS       DASYCU010000060         5646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4943633, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000060 DASYCU010000000
VERSION     DASYCU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4943633"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2624)
                     /locus_tag="VGM24_01195"
     CDS             complement(114..2624)
                     /locus_tag="VGM24_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4154002.1"
                     /translation="MSKDFTKSPGPPRLAALIFLLLCPCLWVAAQKPGAIISGKVLDE
                     NENPLANVSIIQLGRETGIRSSDSGSFRMHVPADKAFALVFSYTGYKTVQQNFLLNEK
                     EEEVILIRLEKSNQVLPGVTVSDERDRKEAGLIRINPKDAIHIPTPTGGIESLIKVFV
                     GSTNELTSQYSVRGGNYDENLIYVNDFEIFRPYLVSNAQQEGLSFINPEMTRNVSFYN
                     GGFQAKYGDKISSVLDIQYKKPKQFGGSAYVGLLEQGMELEGSSGNNRFTYLVGLRNR
                     SNKDLLSSQETKGSYVPSSADIQGLFTYQLNQKNSIELFSVFSKTAFSLIPQSAQLTT
                     SVFSPYFSSNLALDVFFNGQEKDRYETSLLGLTWNQQLNKDLKLKWMVSEFSDQEKES
                     YDIAGDYLFGERDFDQSSSEFGAIVNPLGAGRYQNYARNLLNISILNLSHKGYLSKKN
                     HYLQWGLTAARETVHDHLNEWQYQDSAGYSLPDQPGSLLLFSAQKGDADLVTGRFSGF
                     VQDNISFNGAHAFTVQAGARFNYNTLNREMLVSPRLDFSWKPKKTNRDVVYRGAIGIY
                     DQPPFYRELRKPDGSLNKELKAQKSWQLTGGADFNFHANDKPFRLSVEAYYKNMWDVN
                     PYDVNNVHIQYFGTNNARAYAMGIEGRLFGNLVKDAESWISIGFMRTREKIDGAQYYD
                     YALDSLNRPVDSSLVRQGWIRRPTDRLFTLGMFIQDYLSTNKNFKVYLNLLYGSNLPF
                     NIPGSVKYRNALVIDPYIRADIGFSALLLDNDKSNRRSHSPFRNLENIWATLEIFNLI
                     DRENTISYLLIKDFSNTTYAMPQRLTPRLINLKLVTRF"
     gene            complement(2631..3278)
                     /gene="rpe"
                     /locus_tag="VGM24_01200"
     CDS             complement(2631..3278)
                     /gene="rpe"
                     /locus_tag="VGM24_01200"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY4154003.1"
                     /translation="MPLVAPSLLASDFLHLAAVCELVNESEADWFHLDVMDGRFVPNI
                     SFGIPVIEHIRKAAKKICDVHLMIEEPEKLTPAFKKAGADTLIVHLETCRNLHSNVQQ
                     IRSLGMKPAVAINPHTPVEFLQDILPDLHHVLIMTVNPGFGGQSFIPHSLEKVREMRK
                     MIVKTGKEILIEVDGGITLENAPSLVEAGADVLVAGSTVFQARDPKQIIHLLKNC"
     gene            complement(3377..4411)
                     /locus_tag="VGM24_01205"
     CDS             complement(3377..4411)
                     /locus_tag="VGM24_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4154004.1"
                     /translation="MAPSFRAYFVISVLFFLTGLAGCGNAPENFPGPAVERNPAYAAL
                     NDSIRSFPKDASLYLRRARRLSLENAHELAYSDFKKAWSLQPSVETGLPYAANLQILG
                     KTSERLALLKAGNRQFPENQQMQRLLADAYSGSGNRDLALRQYGEILAKDPKDFETWY
                     EQGLLLEQLKDTAAAISSLQKAYALQAVATYGLELAHLYAEQKNAKALEICNNILKAD
                     SASQLIDPLFIKGIYYSNISLYPKAIAQFDSCIQRDWKTTDAYLEKGIAYFRMQNYDA
                     AIHTFNMAITVSNTDPDAYYWLGRSYEAIHRKADAISNYQRTLSLDRRFTEAKEAIER
                     LKSGFGDASH"
     gene            4492..4950
                     /locus_tag="VGM24_01210"
     CDS             4492..4950
                     /locus_tag="VGM24_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154005.1"
                     /translation="MQAVLLEVHSILRWVILLLLLLSIIQSFAGWRKKRELREGDAKL
                     WLFTMVSVHITLLIGLYLLFFGRFGVFSAGLPEGVELMKDKFYRFFWIEHPVGMLIAT
                     TLVTLARGVVRKQIQDPLKYKRAFWLFLFALIIILAVIPWPGRNIVGRPL"
     gene            5045..5443
                     /locus_tag="VGM24_01215"
     CDS             5045..5443
                     /locus_tag="VGM24_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154006.1"
                     /translation="MIKFHEIKNGDIVQVDYEGQRFEGEVQELDHEDKLVRVQTGEQE
                     FWYAPVDLFPIPLDDNQLTKLKFSKEQNGDQSVKYMRGPFRILLPEKDQFGQFEMWYR
                     EDRRHISHPIGVHELQNFYHQMTKVDLTRD"
     gene            5561..>5646
                     /gene="infA"
                     /locus_tag="VGM24_01220"
     CDS             5561..>5646
                     /gene="infA"
                     /locus_tag="VGM24_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779202.1"
                     /note="stimulates the activities of the other two
                     initiation factors, IF-2 and IF-3; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY4154007.1"
                     /translation="MAKQALIKQDGTIVEALSNAMFKVKLENG"
BASE COUNT         1356 a         1340 c         1429 g         1521 t
ORIGIN      
        1 ttgccaattc ttaagtgatt gattataagt tcgttgtcaa tagcctcgcg gggtgaattc
       61 tccctatttg ggaaatttta attctttttt ggacaagatt cgcttccagc aggctaaaac
      121 cgggtaacga gtttcagatt aatcagccgc ggtgtaagcc gctgaggcat ggcataagta
      181 gtattcgaga agtccttgat gaggaggtac gaaatggtat tttcccggtc aattaaatta
      241 aatatctcca gggtagccca gatattttcg aggttccgga acgggctatg acttcttctg
      301 ttgcttttgt cattgtccag caacagcgcg ctgaaaccaa tatctgcgcg aatatatgga
      361 tcgatcacga gcgcattccg gtattttacg ctgcctggaa tattgaaggg caggttgctt
      421 ccatacagga ggttcagata gaccttgaag tttttattgg tggaaagata atcctgtatg
      481 aacataccca gcgtgaacag gcgatcggtt ggcctcctga tccatccctg ccggaccagg
      541 ctgctgtcca ccggccggtt cagggaatcc aaagcataat cataatactg agctccatca
      601 attttttccc gggtgcgcat gaatcctatg ctgatccagc tttcggcatc ttttaccagg
      661 ttgccgaaaa gccttccttc aatgcccatg gcataggcgc gggcattatt cgttccgaaa
      721 tactggatat gcacattgtt tacgtcgtag ggattcacgt cccacatgtt tttataatag
      781 gcttcgacgg ataagcgaaa aggtttgtcg ttcgcatgaa aattgaaatc tgcgcccccc
      841 gtaagctgcc agctcttttg tgctttcagt tctttattga gagagccgtc cggcttccgg
      901 agttcccggt aaaatggagg ctgatcatat atgccgatgg ccccgcgata taccacatcc
      961 cggttggttt ttttaggctt ccaggaaaaa tcaagccgcg gagaaaccag catttcgcgg
     1021 ttgagtgtgt tgtaattgaa tctagctccg gcctgtaccg tgaacgcatg tgcaccgtta
     1081 aaggaaatat tatcctggac aaatccgctg aaccggccgg tgaccaggtc ggcatctcct
     1141 ttttgcgcac tgaacagaag cagggaacca ggctggtccg gcaatgaata tccggcagaa
     1201 tcctgatact gccactcatt caggtgatca tgcacggttt cccgggcagc agtcagcccc
     1261 cactggagat agtggttttt cttactgaga tatcccttgt gtgataaatt caggatggaa
     1321 atattcagca ggttgcgggc gtaattctga tatcggccgg ctcccagggg attgacaatt
     1381 gcgccgaatt ccgagctgga ctgatcgaaa tccctttctc cgaacaaata atctccggca
     1441 atatcgtagg actctttttc ctggtcgctg aattccgaaa ccatccattt taatttcagg
     1501 tccttattca attgctgatt ccaggtaagc cccaggagac tggtctcata acgatctttt
     1561 tcctggccat taaaaaagac atcaagggcc aggttggagg aaaaataggg agagaaaacc
     1621 gaggtcgtca gttgggctga ttgcgggatc aggctgaagg cggtctttga aaacacggaa
     1681 aataattcaa ttgaattctt ttgattgagc tgataggtga acagcccctg gatatcggct
     1741 gaggaaggca catagctccc cttggtttcc tgtgaactga gcaggtcttt attgcttcgg
     1801 ttgcgcagtc cgaccaggta ggtgaaccgg ttatttccgg atgaaccctc cagctccatg
     1861 ccctgttcca gtaatccgac ataggcagaa ccgccaaact gttttggttt tttatattga
     1921 atgtcgagca ccgaggaaat cttatctccg tatttagcct gaaatccgcc attataaaaa
     1981 cttacgttcc gggtcatttc cgggttgata aagctaaggc cttcctgttg cgcattactg
     2041 acgaggtaag gacggaaaat ttcaaaatca tttacataga tcaggttctc atcatagttt
     2101 ccgccgcgca cggaatattg ggaagtcagt tcgttggtgg aaccgacaaa tacttttatc
     2161 aggctctcaa taccaccggt gggcgtgggg atgtggatgg cgtcttttgg attaatgcgg
     2221 atgagcccgg cttctttccg gtcccgctca tcgcttactg taacgccggg caaaacctga
     2281 ttggatttct cgagccggat aaggatcacc tcttcctcct tttcattaag caggaaattc
     2341 tgttgtacgg ttttgtaacc ggtataggag aagaccagcg caaatgcctt gtcggccgga
     2401 acgtgcatcc ggaaactgcc tgaatccgaa ctgcggattc cggtttcacg gcccagctgg
     2461 ataatggata cgttggccag cggattctca ttttcatcca gcacttttcc ggaaatgatg
     2521 gccccgggct tttgtgcggc gacccagaga cagggacaca gcaggagaaa tattaaagca
     2581 gccagccttg ggggtcccgg agatttggta aagtctttcg acatttcggc ttagcagttt
     2641 ttaaggaggt ggattatctg tttcggatcc ctggcctgga atacggtaga tccggccacg
     2701 agaacatctg ctccggcctc cacgaggctg ggtgcattct ccagcgttat tccgccatcc
     2761 acttcgatca ggatttcttt tccggttttc acgatcattt ttctcatctc ccgtactttt
     2821 tccagggaat ggggaatgaa ggattgtccg ccgaatcccg gattgaccgt catgatcagc
     2881 acgtgatgaa gatcgggtaa aatatcctga aggaattcaa cgggcgtgtg tggattgatc
     2941 gctactgccg gtttcattcc gagggaccgg atctgctgga cattgctgtg caggttccgg
     3001 caggtttcca ggtgaacgat cagggtatcg gcccccgctt tcttaaatgc gggggtgagt
     3061 ttttccggtt cttcgatcat caggtgcaca tcacagattt tttttgccgc tttgcggatg
     3121 tgttcgataa ccggaatacc gaaactgata ttgggaacga aacggccgtc catcacatcc
     3181 aggtgaaacc agtccgcctc actttcattc acgagttcac aaacggcagc caggtgaaga
     3241 aagtcggatg cgagcaggga aggagctaca agcggcatga aaagatttac tttaattgaa
     3301 aaattgaaaa tcaggaatgg cgaacggaaa gtcggaacgc atccctgcag ggcttttcgg
     3361 ctggacaaat gtactcttag tgagaggcat ccccaaatcc ggatttaagt ctctcgatgg
     3421 cctcttttgc ctctgtaaaa cgtctatcca gcgaaagcgt cctctgataa ttactgatgg
     3481 catctgcttt ccggtggatc gcttcgtagc tcctgcccaa ccagtaatag gcatcgggat
     3541 ccgtgttgga aacggtaatg gccatattga acgtatggat ggcggcatca taattctgca
     3601 tgcggaaata ggcaatcccc ttttccaggt aggcatcggt ggttttccag tcgcgctgga
     3661 tacaggaatc aaactgggcg atcgcttttg gatataagct gatgttggaa taataaatcc
     3721 ccttgatgaa aagcggatct atcagctggg aagcagaatc ggccttcagg atattattgc
     3781 agatctcgag tgcctttgca tttttctgtt ccgcatagag atgggccagt tcaagcccgt
     3841 aggtagccac tgcctgcaat gcatacgctt tttgcaggga gctgatcgcg gccgccgtat
     3901 ccttcagttg ttccagcagg agcccctgct cgtaccaggt ctcaaaatct ttaggatctt
     3961 ttgccagtat ttccccgtat tgtctgaggg cgagatcccg gtttccggat ccggaataag
     4021 cgtctgccag aagcctttgc atttgctggt tttccgggaa ctgacggtta ccggctttca
     4081 gcagggccag tctttcggac gttttgccga ggatctgcag attggcggca tagggcaggc
     4141 cggtttccac cgaaggctgc agggaccagg cttttttaaa gtcgctgtag gccagttcat
     4201 gggcattttc cagagaaagc ctcctggccc tgcgcaggta gagggaggca tcttttggaa
     4261 atgaacggat actgtcgttg agcgcggcat aggccggatt gcgctcaacc gccggcccgg
     4321 ggaaattttc cggcgcattt ccacagcccg ctaggccggt taaaaaaaag agaacggaaa
     4381 taacaaaata agccctgaag gaaggtgcca tccggcaaaa ctacaatcat tccgcctggt
     4441 tcctaaagga tggcaacccg aaaacatcta atttcaacct aaattttttt tatgcaagct
     4501 gtccttttag aggttcacag tattctccgg tgggtaatcc tcctgctgct cctgctttcc
     4561 attatccaga gttttgccgg ctggcgcaag aaaagggaat tgcgggaagg ggatgccaag
     4621 ctttggcttt ttaccatggt cagcgtgcat atcactctgc ttatcggatt atatcttttg
     4681 ttttttggcc gttttggcgt cttcagcgcc ggacttccgg aaggggttga gctgatgaaa
     4741 gacaaattct accggttttt ctggatagaa catccggtcg gcatgctaat cgcgaccacc
     4801 ctggtcaccc tggccagggg cgttgtccgg aaacagatcc aggaccccct gaaatataaa
     4861 cgggcattct ggcttttcct gttcgccctg atcattattc ttgcggtcat tccctggccg
     4921 ggcagaaata ttgtcgggcg tccgctctga tttcaaccct tacattaaaa cccggacgaa
     4981 attcccgggc tgcgctgcct tttagtataa tttttttata ttcatatcct caaaagacgg
     5041 caatatgatt aaatttcacg aaatcaaaaa cggcgatatt gttcaggtag actatgaggg
     5101 tcagcgcttt gaaggggagg tgcaggaact ggaccatgaa gacaagctgg ttcgcgttca
     5161 gaccggcgaa caggaattct ggtatgctcc ggtggacctg tttcccatcc ccctggatga
     5221 taatcaactc accaaattaa aattcagcaa ggaacagaac ggagaccagt cggtcaagta
     5281 tatgaggggt ccctttcgca tcctcctgcc ggaaaaagac cagttcggtc aatttgagat
     5341 gtggtaccgg gaagatcgcc gccatatcag tcatcccatc ggggtccatg agctccagaa
     5401 tttctaccac cagatgacaa aagtggacct gacccgggat taagccctga ttttccgggt
     5461 tttcggccca ttccgggcta tcgcgctggt tatatggata tttctcttac gaaattttat
     5521 tctttcgctg agtaatatta tctttgcgct cccaaaatct atggctaaac aagcgctcat
     5581 taaacaggat ggtactattg tggaagcact ttctaatgcc atgttcaagg tgaagctcga
     5641 aaacgg
//
LOCUS       DASYCU010000061         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4221256, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000061 DASYCU010000000
VERSION     DASYCU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221256"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VGM24_01225"
     CDS             <1..1393
                     /locus_tag="VGM24_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154008.1"
                     /translation="LVTFLAGVLALVLVQFAMPFFRTITGKALSLRTFGILPTIGFTG
                     LFSIIVGLISGIYPSVFLSRFKTTPALKGNMGNMSGTILFRKSLLVLQFVITVFMISA
                     SFVIYQQLKYVTHSDLGFNKDQVLTFHIDDPNLRAQIPAIKAQLLQNPLIEGVAAAGN
                     PIGNNDLGKRGYRYEKNDKAIAQNTKAAQELIVDADYIPTMQIKLAEGRNFSSEIPTD
                     QTNSILVNETLVKELGWTHPVGMKMQKVGGSEEGMKTVIGVFRDFHTYSLQHKVQSLV
                     LTMPPNVREQDNLYVRVAKGKSKQALAWLDQVYKSFDAGNPAEYHFLDQNFARQYEAE
                     QKQGTLAFAFTQLAILLSVLGLFGLVTFTAQQLTKEIGIRKVLGASIVNIVQLVSKDF
                     LKLVTLAAFIALPLSWFAMQRWLLDFAYRVPLHWWVFVIAGLISAGIALITLSIRAVQ
                     AARVNPVKSLRAD"
     gene            1571..2170
                     /locus_tag="VGM24_01230"
     CDS             1571..2170
                     /locus_tag="VGM24_01230"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEY4154009.1"
                     /translation="MNAEQNPVIIKDTHANPAPLGLFGFGMTTVLLNLHNAGIYEMNS
                     MILAMGLFYGGTAQVIAGIFEYKKNNTFGMTAFISYGFFWISLVALIFMGKWGWIPSA
                     TGSAMVAYLILWGIFTLLLFFGTFRLNKALQFIFGTLTLLFFLLAASDATKNESLQHF
                     AGYEGIVCGASAMYAAAAVVLNELYGRTVLPLGMAKARQ"
     regulatory      2276..2480
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2586..>3269
                     /locus_tag="VGM24_01235"
     CDS             2586..>3269
                     /locus_tag="VGM24_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY4154010.1"
                     /translation="MQEETELLLKENKDRFVILPINYPRIWEMYKKHEASFWTAEEID
                     LSGDQKDWQSLNDGERHFISHILAFFAASDGIVNENLAVNFMSEVQLPEARCFYGFQI
                     MMENIHSETYALLIDAYIRDPKEKDRFFHAIDTVPVVKKKAEWALRWIQNGSFAERLV
                     AFAAVEGIFFSGSFCSLFWLKKRGLMPGLTFSNELISRDEGLHCEFACLLYGMLSKKL
                     SEEAVHAIIG"
BASE COUNT          774 a          782 c          823 g          890 t
ORIGIN      
        1 gctggtcacc tttcttgccg gagtgctggc cctggtgctg gtgcagtttg cgatgccttt
       61 cttccggacg atcacgggca aggccctgtc tctccggact tttggcattc ttcccacgat
      121 cggctttaca gggttgtttt ccataatagt cgggttgatc agtggcattt acccttctgt
      181 ttttttatcc cggttcaaaa ccactcctgc gctgaaaggc aatatgggaa atatgagcgg
      241 aacgatcctg ttccgcaaat cgctgctggt attgcagttt gtgattaccg tgtttatgat
      301 ttccgcgtct tttgtgatct accagcaatt aaagtatgtt acgcattccg acctgggttt
      361 taataaggac caggtactga cttttcatat tgatgacccg aacctgcggg cacagatccc
      421 ggccatcaag gcccagttat tacaaaaccc cctgattgaa ggcgttgccg ccgcgggaaa
      481 cccgatcggg aacaatgatc tggggaaaag gggataccgg tatgagaaga acgataaggc
      541 cattgcgcaa aataccaagg cagctcagga actcatcgtg gatgcagact atattcccac
      601 gatgcagatc aaactcgcgg aaggaagaaa tttttcatcg gaaattccga ccgaccagac
      661 gaattccatc ctggtgaatg aaacgctggt aaaagaactg ggatggaccc atccggtcgg
      721 gatgaaaatg caaaaggttg gaggatcaga ggagggaatg aaaaccgtta tcggcgtgtt
      781 ccgggatttt catacctatt cattacagca taaagtgcag tccctggtac tcaccatgcc
      841 gcccaatgtt cgggaacagg ataatttata tgtacgtgtg gcgaagggga aatcgaagca
      901 ggcattggcc tggctcgatc aggtttacaa aagttttgat gccgggaatc cggcggagta
      961 tcattttctg gatcagaatt ttgcaaggca gtatgaagcc gagcaaaaac agggaacact
     1021 ggcttttgcc tttacgcagt tggccatcct gctatccgtg ctgggattat tcggcctggt
     1081 cacctttacc gcccagcagc tcaccaaaga gatcggcatc cgcaaagtgc tgggtgcttc
     1141 catcgtgaat atcgttcagc tggtatccaa agattttcta aagctggtga ccctcgccgc
     1201 tttcatcgcc ctgccgcttt cctggtttgc catgcaacgc tggctgctgg attttgccta
     1261 ccgggttccg ttacactggt gggtttttgt gattgccggt ctgatttctg cggggattgc
     1321 gctcattacg ctgagcattc gcgcggttca ggctgcgcgg gtgaacccgg ttaaaagcct
     1381 gcgggcggac tgatatctgc ggatggcctt ctctatattt tctccggggt tttcccgttt
     1441 tctgcttccc cgaactccaa acgggaataa gccgggattt ttaaaatcct gacccacaat
     1501 ctgtgcggcc atattgaatt aagcattatt tttagacggt caacgattgt ctaaaccata
     1561 aaacaacaat atgaatgccg agcaaaaccc cgttatcata aaggataccc atgcgaatcc
     1621 cgcaccgctg ggattatttg gatttggcat gaccacggta ttactgaacc tgcacaatgc
     1681 gggaatctat gaaatgaatt ccatgatcct tgccatggga cttttttatg ggggaaccgc
     1741 ccaggtgatc gcgggaattt ttgaatacaa gaaaaataat acgttcggca tgacggcctt
     1801 catttcctat ggtttcttct ggatcagcct ggtggccctg atcttcatgg ggaaatgggg
     1861 atggatccct tccgctaccg gatccgccat ggtcgcttat ttaatcctct ggggaatttt
     1921 tacgctcctg ttattcttcg gcacgttccg cctgaacaaa gcgctgcaat tcattttcgg
     1981 gaccctgacc ttactcttct tcctgctggc tgccagcgat gcaaccaaaa atgaatccct
     2041 ccaacatttt gcgggttacg aaggaatcgt ttgcggcgct tccgccatgt atgcagccgc
     2101 tgcggtcgtc ctgaatgaat tgtacggaag aacggtgctg cccctgggga tggctaaggc
     2161 ccggcagtga tttgatcatc cggattccgg caggacagga gaaatttttt tccccatcct
     2221 gtgcgtaatt catcttccat tctattttta tcaggtctat attcgttatc gctcttgcag
     2281 ataatttgat atcggtgcag ttgatcaata cctgttgatc agcctgctta aaagggaacc
     2341 gtgtgtaatc cacgggctgt cgggcaactg taagtaacct caggctgatt cgttttattc
     2401 cattgcttct ttcagaacgg agcgagaagg aagatcagct gttacgagcc aggagacctg
     2461 ccgatttcag ttattgcccg gaaccttccc ggaaaaggta tcggcaaaga tgatcttccc
     2521 ctgatcttca ctggaatttt tgtctcttgt cgtttcacat tttttaactg tttaaactca
     2581 tgcacatgca ggaagagacg gaattattac tgaaagaaaa caaggaccgt tttgtgatcc
     2641 ttcccataaa ctacccccgg atctgggaga tgtataaaaa acatgaggcc agcttctgga
     2701 cggccgagga gatcgacctg agcggcgatc agaaagactg gcaatcgctc aatgatggcg
     2761 agcgacattt catttcccat atcctggcct tttttgcggc cagcgatggt atcgtgaatg
     2821 agaatctggc ggtcaatttt atgagcgagg tgcaattgcc ggaagcgcgc tgtttttatg
     2881 gttttcagat catgatggaa aatattcatt ccgaaaccta tgccctgctg atcgacgcct
     2941 atatccgtga tcccaaagag aaggaccggt tttttcatgc catcgatacc gtaccggtcg
     3001 tgaagaaaaa ggccgaatgg gccctgcgct ggatacagaa cggatccttt gcggaacgcc
     3061 tggtggcctt tgcagccgta gaaggaattt tctttagcgg aagcttttgc tctcttttct
     3121 ggttaaagaa gcggggcctc atgcccggtc tcaccttcag taatgaactg atcagccggg
     3181 acgaaggact tcattgcgag tttgcctgtc ttttatacgg catgctgagt aagaaacttt
     3241 cggaggaagc ggtccatgcc atcatcgga
//
LOCUS       DASYCU010000062         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4221289, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000062 DASYCU010000000
VERSION     DASYCU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221289"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="VGM24_01240"
     CDS             complement(<1..1851)
                     /locus_tag="VGM24_01240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEY4154011.1"
                     /translation="MRKFLIPLLAAVGLLTVGDPALAQKPTAYVDPFIGSGGHGHVFV
                     GASVPFGAVQAGPDNFYKGWDWCSGYHYGDSVLIGFAQTHLSGTGIGDLGDVLIMPYT
                     GAVKTEKGTETIRGNGYASRYSHRQEQARPGYYSVKLLDYDVDVELTASERAAFHQYH
                     FPAGKEAHVIIDLKEGINDRATDTYIRRVDQYTLEGYRFSKGWAKDQRLFFAIRSSLP
                     LEQFSVYDQDRLLETDSGRGTAIKGLISFAHAPDTLKLKVGISPVSAANALANIDAEI
                     PGWDFQRTAREADEKWNRELSKVEVETKNEKDKRIFYTALFHTMIDPALFNDHNRDYR
                     GADKKIYVKAPFNNYSVFSLWDTYRAENPLFTILQPQRVNDLINSMLAIYRQQGRLPI
                     WHLMGNETGTMPGISSLQVIAEAFLKGFKGFNGDSAWTAINASAKSDPLGMKYVRDLQ
                     WIPSNEQRSSVARAMEYAVSAGSIALMAKKMGRIDDFHYFQKRAENYKLNFDTGTQFF
                     RGRNSDGSWALPFDPFKTTGAAARAYSEGNAWQYLWLAPQDVKGLIATLGGEKKFLAR
                     LDSFFVAEPAVKDTAVLSDITGTIGQYAHGNEPGHHIIYLYACAGEPRKTA"
     gene            complement(2029..2505)
                     /locus_tag="VGM24_01245"
     CDS             complement(2029..2505)
                     /locus_tag="VGM24_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY4154012.1"
                     /translation="MKLTNQIAQQFRDVHFGGNWTASNLKESLSDINWQQATVRLYSL
                     NSIAALVYHMNYYVSAVLLVLDGNPLRTGDKYSFDHPPIQSREDWDKLLDKTWADAEK
                     FAARIEQFPESRLEDDFSGNKYGNYYRNIQGITEHIHYHLGQIVLIKKILLQTDQN"
     gene            2736..3035
                     /locus_tag="VGM24_01250"
     CDS             2736..3035
                     /locus_tag="VGM24_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154013.1"
                     /translation="MRTQFFIAVNIKTASGPETIARFRIGNNPEQARQIYYLLKGDDT
                     LIETNNLFLDLMEMKDGLPVNLKMISCTLDQAAENCRIIMNELSMFSSLEEQGKT"
     gene            complement(3046..>3364)
                     /locus_tag="VGM24_01255"
     CDS             complement(3046..>3364)
                     /locus_tag="VGM24_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HEY4154014.1"
                     /translation="RSGILVVRLWPGEEQLENGKPVQLKLNGVMKTFRYGEKIFLRAG
                     ERITLLPRVGHEFHPESDECIIGEVSTHNDDLTDNFFENKAIGRFSSIVEDEPAVARL
                     VNE"
BASE COUNT          822 a          894 c          755 g          893 t
ORIGIN      
        1 tgctgtcttg cggggttcgc cggcgcaggc gtataaataa ataatgtgat ggcccggttc
       61 attaccgtgc gcatactgac ctatggttcc ggtgatatcg ctgaggacgg ccgtatcctt
      121 cacggccggt tccgcgacga aaaaggaatc caggcgggca agaaattttt tttcaccgcc
      181 aagcgtggct atcagcccct tcacatcctg cggcgccagc cacaggtatt gccaggcatt
      241 gccttctgaa taagcccggg cggccgcacc ggttgttttg aacggatcga atggcagcgc
      301 ccagctgcca tctgaattcc ggccgcggaa gaactgcgtg ccagtatcga aatttagctt
      361 atagttctcc gctctttttt gaaaataatg aaagtcgtca atccttccca ttttttttgc
      421 catgagcgcg atgcttcctg cgctgacagc atattccatg gccctggcca cggaactgcg
      481 ctgctcattg gagggtatcc attggaggtc ccggacatac ttcattccca ggggatccga
      541 cttcgccgaa gcgtttatcg ctgtccaggc agaatcgcca ttgaatcctt taaatccttt
      601 caggaaagcc tcagcgatca cctggagact gctgatcccc ggcatggttc cggtttcatt
      661 gcccatcagg tgccaaatgg gcagcctccc ctgctggcga taaatggcca gcattgaatt
      721 gatcaggtcg ttcacccgtt gcggctggag gatggtgaaa agcggatttt ctgcacggta
      781 ggtatcccac agcgaaaaaa cggaatagtt attgaaaggc gcctttacat atattttttt
      841 atccgccccc cggtagtccc ggttatgatc gttgaacaag gccgggtcaa tcatggtatg
      901 aaacagggcc gtatagaaaa tgcgtttgtc cttttcgttt tttgtttcaa cttccacttt
      961 cgacaattcc ctgttccatt tttcatcggc ttcccgggcc gttctctgaa aatcccagcc
     1021 ggggatctcc gcatctatat tcgccagcgc attcgcagcg cttacgggag agatccccac
     1081 cttcagtttc agtgtgtccg gcgcgtgcgc aaaactgatc agccctttta ttgctgtgcc
     1141 ccgtccgctg tctgtctcca gcagtctgtc ctgatcgtat acgctgaatt gttcgagcgg
     1201 cagggaggaa cggatggcaa agaacaagcg ctgatctttt gcccatcctt tggaaaaccg
     1261 gtatccttcc agcgtgtatt ggtcaacccg tctgatatag gtatcggtgg cccggtcatt
     1321 aatcccctct ttaagatcga tgatcacatg cgcttcctta ccggcaggaa aatgatactg
     1381 atggaatgcg gccctttcgg aagctgtcag ttccacgtcc acatcataat ccaggagttt
     1441 aacggaataa tatccgggcc tagcctgctc ctggcggtgc gaatagcggg aagcataacc
     1501 gtttccccgg atggtctctg tccctttttc cgttttcacc gctccggtat agggcatgat
     1561 gagcacatcg cccagatcgc caatgccggt accgcttaaa tgcgtttgcg caaaaccgat
     1621 cagcacgctg tctccgtaat gatatccgga acaccagtcc cagcccttgt aaaagttatc
     1681 cggccccgcc tgcacggcgc cgaaaggaac acttgcgccg acgaaaacat gaccatgtcc
     1741 ccccgagccg ataaagggat ccacgtatgc ggttggtttt tgggccagag ccgggtcacc
     1801 aacggtcagc aaaccaaccg cggccaacaa gggaattaga aattttctca ttcaggctgt
     1861 ttaatgcaaa tatccattca ccgtaaaaaa aataagcatc cgttcccaaa taaaagatcc
     1921 tgagattaat aaaaacacta ttaacagatt aattctctcc gattgggaca atattttttt
     1981 ataccgaatg cgctatagct acctttcttc cgacgggcca ttcccgggtc agttttggtc
     2041 tgtctgcagg agtatttttt ttatcagtac gatctggcct aaatgataat gaatatgttc
     2101 tgtaattccc tgaatatttc tgtagtaatt cccatattta ttcccggaaa aatcatcctc
     2161 cagcctgctc tccgggaact gctcaatccg cgcggcaaac ttttccgcat ccgcccaggt
     2221 tttatccaat aatttgtccc agtcttcccg agactgtata ggcggatggt caaagctgta
     2281 cttatcaccg gtccggagcg gattcccatc caggaccagc aggaccgcac tcacataata
     2341 attcatatgg tagaccaggg ctgcaatgct gttcagggaa tataatctca ctgtcgcctg
     2401 ctgccagttt atatcactca ggctttcttt caggtttgac gccgtccagt ttccgccaaa
     2461 atgaacatcc ctgaactgct gtgcgatttg gtttgtcaat ttcatattat agggattttt
     2521 cagaatagaa acgatcgtag agttactata aaatcgagtg ttcccgcgtt ataataaaag
     2581 gcagcagtgg cttaattttg ctctatccaa tccaatgaaa gatctatatc cggaaaacca
     2641 cctgtccaat tcctaagtaa aaacaagaat tcattccttg aaaaaccgct gatttattct
     2701 ggcggttttt tattttctgt tccaaatcca aggtcatgag aacgcaattt tttatcgcgg
     2761 tcaatataaa aacggcatcg ggaccggaaa ccattgcccg gtttcgcatc ggcaataacc
     2821 cggaacaggc ccggcagatc tattatctgc tgaaagggga tgataccctt atcgaaacca
     2881 acaacctctt tctggactta atggaaatga aggacggcct gccggtaaat ctcaagatga
     2941 tcagctgcac cctggatcag gccgctgaaa actgcaggat cattatgaac gagctatcca
     3001 tgttctccag cctggaagaa cagggaaaga cctgaatcaa accgcttatt catttaccag
     3061 cctggcaact gccggttcat cttccactat actggaaaag cgtccgattg ccttattttc
     3121 aaaaaaatta tccgttaaat catcattatg ggtagagact tcccctataa tgcattcatc
     3181 cgattccgga tggaactcat gtccgacacg gggaagcagg gtgatccgtt caccggcacg
     3241 aagaaatatt ttctccccat accggaatgt tttcatcacc ccgtttaatt tcagctgcac
     3301 cggctttccg ttctccagct gttcttcccc gggccaaagc cgaaccacca ggattccact
     3361 cctg
//
LOCUS       DASYCU010000063         3431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2771507, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000063 DASYCU010000000
VERSION     DASYCU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2771507"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..785
                     /locus_tag="VGM24_01260"
     CDS             69..785
                     /locus_tag="VGM24_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154015.1"
                     /translation="MRTGGRIPAHLFFLLLSSTGFAQQPFSGRVFKKGSTEVLAGINV
                     TNLTGQKHNVSDLGGNYRIPAAPGDSIIFSSAGYLPDTIVLNPACFADPYPVFLTAHI
                     VALPPVTVGEESGYQVDSLQRRKDYGFIYDKKHPVKLWNEKRPGDEPGFSFSPAGFFS
                     KTEKEKRRLKKRLQQEEEDDYIDLRFPRNRVAQLTRLTGDSLQQFMIRYRPSYRFCRN
                     ASNQDMFFYINDKLILFKKG"
     gene            complement(828..2339)
                     /locus_tag="VGM24_01265"
     CDS             complement(828..2339)
                     /locus_tag="VGM24_01265"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEY4154016.1"
                     /translation="MKELLKCSFIALFFLVLPALAWCQNKTITYTESKEDFSNPERGF
                     YLPTGTRASHFVPLDGEKIKAYRLKPQKPGRASYSVEASLILRGYELDSFKDRPLSDS
                     FLTDLQKDFDEVREAGLKMILRFAYINKTHAGDCGDQYHICPPYGDAPEAIVLTHIQQ
                     LKPLFQKNEDVIAVLQMGFIGIWGENFYTDYFGDASANGPGKIMDSSWVHRKEVLKAL
                     LEALPADRMIQVRTPQIKQKYVYGPSAAVTSAPLQESEAFSESDKARIGFHNDCFLAS
                     ADDYGTYYDYGSSAQPKQPATEPLRKYIEADTRFTVVGGETCDDAFSPQNDCAPAGYA
                     EEEIRQMHYSFLNSAYNTSVNNDWDSGGCLDNIKRKLGYRFVLRKAQFPQKVGRAGSL
                     NCRLFLDNIGYASPYNPRPVEMIFRNIVTGKEFMAALKLKPQLLFPGSHQIPLVIQMP
                     AGITPGAYELYLFLPDAGSSISKRPEYAIRLANENMWEESTGYNDLHQELQVK"
     gene            complement(2456..3130)
                     /locus_tag="VGM24_01270"
     CDS             complement(2456..3130)
                     /locus_tag="VGM24_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY4154017.1"
                     /translation="MKKKVIVMLRLSAFFMATLLFASLNASAQEQQTSMESGLRPKLG
                     IKGGLNLTNLYVSDVSKEHMKAGFNAGLFAKLPVTQGFSIQPELLYSVKGAKDDYNNV
                     VQGSGEYRFNLGYIEMPVLAVVNLARNFNLHAGGYAAYLVNANVKDVNSDGDIQGANE
                     LNADNFKRWDVGLVGGLGFDIENFTIGARYNYGLTNIGKSGSLSGDLTKNSKNAGVSI
                     YLGFGF"
BASE COUNT          881 a          828 c          795 g          927 t
ORIGIN      
        1 aaacggccgt ctttcaatga ttaaatttat tcttcaaacc gcgctcgcaa attataaccc
       61 ttatttgtat gcgaactgga ggaagaattc ctgcccatct gtttttcctg ctgctttctt
      121 ccaccggatt tgcccagcag ccattcagcg gcagggtttt taaaaaagga agtacggagg
      181 tactggccgg cattaatgta acgaacctta ccggacaaaa acataatgta tccgatctgg
      241 ggggaaatta caggatcccc gcagctcccg gagattccat cattttttct tctgccgggt
      301 acctgccgga tacgattgtg ctgaacccgg cctgctttgc cgatccgtat cctgtttttc
      361 tgacagcaca tattgtggct ttgcccccgg taaccgtggg agaagaatcg ggttatcagg
      421 ttgactcgct gcaaagacgc aaagactacg ggtttattta tgataaaaag cacccggtaa
      481 aattatggaa tgaaaaaaga cctggtgatg agcccgggtt tagctttagt ccggccggtt
      541 ttttttccaa aacagaaaag gaaaaaagga gattgaagaa acggctgcaa caggaggaag
      601 aggatgatta catcgatctc cggtttcccc gcaaccgggt tgcacaatta accagattaa
      661 caggagattc ccttcagcag ttcatgatcc ggtacagacc ttcctaccgg ttttgccgga
      721 atgccagcaa ccaggatatg ttcttttata taaatgacaa gctgatcctg ttcaaaaaag
      781 gctgaaggaa tttcccgcgt tccgaagacg gtttggctga gcgttcttta ttttacctgc
      841 aattcctgat gaagatcatt atatccggtt gattcttccc acatgttttc attcgccaga
      901 cggatggcat attcgggtct tttagagatc gaacttccgg cgtcaggcag gaacaaatac
      961 aattcatagg ctccgggagt tatgccggcc ggcatttgga tgaccagggg aatctggtga
     1021 gaacccggga acaaaagctg cggcttcagc ttcagggcag ccataaattc ctttccggta
     1081 acgatattcc ggaagatcat ttcaaccggc ctgggattgt aaggggatgc atatccgata
     1141 ttatccagaa aaagccggca gttcagcgat ccggcccgtc ctaccttctg cggaaattgc
     1201 gcttttctca aaacaaaccg gtagcctaat tttcgcttga tgttatccag gcagcctccg
     1261 ctgtcccagt cattattcac actggtattg taggcggaat tcaaaaagct gtagtgcatc
     1321 tgccggattt cttcttctgc atacccggcg ggtgcgcaat cgttttgagg gctgaacgcg
     1381 tcatcgcagg tttctccacc cactacggtg aaccgggtgt ctgcttctat gtatttccgg
     1441 agcggttcag tagcgggctg ttttggctgt gcggaacttc catagtcgta gtaagtgcca
     1501 taatcgtccg ccgaagccag aaaacaatcg ttatgaaatc caatccttgc cttatcggat
     1561 tcgctaaagg cctcagactc ctggagcggc gcgctggtta ccgccgcgga aggtccgtaa
     1621 acatattttt gctttatctg gggggtgcgc acctggatca ttctgtcggc tggcagggcc
     1681 tccaggagcg ccttcaaaac ttccttccgg tgaacccagc tgctgtccat aatttttccc
     1741 ggtccgtttg ccgaagcgtc cccgaaataa tccgtataaa aattttctcc ccagatacca
     1801 atgaatccca tctgcagcac ggcgattaca tcttcattct tttgaaacaa gggctttaac
     1861 tgctgtatat gcgtgagcac gatggcttcc ggtgcatccc cataaggcgg gcaaatatgg
     1921 tattggtccc cgcagtctcc ggcatgtgtt ttatttatat atgcgaagcg gagaatcatc
     1981 ttcagaccgg cttcccgcac ctcatcaaaa tctttttgga ggtcggttaa aaaagaatcc
     2041 gacaggggac ggtctttaaa actgtccaat tcatatccgc ggaggataag cgaggcttct
     2101 accgaataac tcgctctgcc aggtttttgc ggcttcaaac gataggcctt tattttttcg
     2161 ccatccaggg gtacaaaatg actggcccgg gttcccgttg gtaaataaaa acccctctcc
     2221 gggttggaaa aatcttcttt gctctcggta tacgtgatgg tcttattctg gcaccaggca
     2281 agggccggca atacaagaaa aaagagcgca ataaaagagc atttcagtaa ttccttcatg
     2341 aaaaaaagat tagccggcct gcgttaaaat cagggctatg aagataggga aagatattcc
     2401 acaaaaaagg cctgtcccaa atagaaacag gcctcttcaa taaaaacatt ccaattcaaa
     2461 atccaaatcc gagataaatg gatactcctg cgttctttga atttttagtc aggtctccgg
     2521 aaaggctgcc cgattttccg atattggtca gcccatagtt ataccgtgcg ccgatggtaa
     2581 aattctcgat atcaaaaccg agcccgccga ccagcccaac gtcccatctt ttaaaattat
     2641 ctgcattcag ttcgtttgcg ccctgaatgt cgccgtcgct gttgacgtct tttacattcg
     2701 cattaaccag gtaggcggca tatcctcccg catggagatt aaaattcctg gccaggttaa
     2761 ccaccgcaag cacgggcatt tcaatgtatc ccaggttgaa ccggtattct ccgcttccct
     2821 gaacaacatt gttgtagtca tcttttgctc ccttcacgct gtataaaagt tccggctgaa
     2881 tggaaaagcc ctgggtaacc ggcagcttgg caaacaaacc ggcgttaaaa ccggctttca
     2941 tgtgttcttt ggaaacatca gaaacataca ggtttgtaag attgagcccg cccttgatcc
     3001 ccaatttcgg ccggagtccg ctttccatgg acgtttgctg ttcctgggcg gatgcgttta
     3061 aagatgcaaa tagtaatgtc gccataaaaa aggcacttaa cctcagcatc acaattactt
     3121 tctttttcat attgatttta tttgtttcca tgcatattca atagttgtgc ctgagcccgg
     3181 gagctttttt atcgtgttta tgagcaattt cactgctgta aacgggttga aattgaagcg
     3241 gttagcagga ggagaaggaa ggcgcacgga gaacggattt gcgttacaac cggtaaccgg
     3301 atgcaccggg gatgagtata tttatcatca ttttaacgcc ccgctgggga tttcaaatca
     3361 tccaataaat tgcatttctt caggccggaa tccgattgca ggaacgggct ctttttgcaa
     3421 actgaaaaat a
//
LOCUS       DASYCU010000064         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3786346, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000064 DASYCU010000000
VERSION     DASYCU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3786346"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /gene="pckA"
                     /locus_tag="VGM24_01275"
     CDS             complement(<1..1544)
                     /gene="pckA"
                     /locus_tag="VGM24_01275"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY4154018.1"
                     /translation="MSVPVMGLPLKKLMNLGLNVTGSIHYQLSPEELISQCLRRKEGK
                     LTDTGALWISTGEFTGRCPKDRYLVKDEISAGNMNWNNYNQPLEETQFDQIFRQVTAY
                     LNQLPEIWVRDSSVCADPAYRLNLRVITETPALNLFAYNMFLRPEEEGFRADWHILAA
                     PGLKLNPASAGIRQQNAVIISFWHQLILLAGTGYTGEIKKSVFSVLNYLLPFQKGVLT
                     MHCAANMGKAGDTAIFFGLSGTGKTTLSADRSRRLIGDDEHGWNGNGIFNFEGGCYAK
                     CIHLKEENEPDIFHAVRTGALLENTGFFPGTDRVNYDDLSKTENTRVSYPIDYIPHSV
                     VPSLGRIPSNIFFLTCDAFGVLPPVSRLTVEQAMYQFISGYTAKIAGTEAGINEPKST
                     FSACFGAPFLPLHPVFYAKMLGEKIRKEQVRVWLINTGWSGGAYGRGSRIPLSYTRAI
                     VSAILDGKLGNCNWKVHPVFHFAMPETCPGLPEKILNPESSWENPAEYQLKARELAGR
                     FIENFK"
     gene            1813..>2056
                     /locus_tag="VGM24_01280"
     CDS             1813..>2056
                     /locus_tag="VGM24_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY4154019.1"
                     /translation="MLSSSIAIFVSNDEMPSNGDALYPFKQNSDLYWLTGIVQEESML
                     ILFPDHPDPQFREVLVLIRPNELKEKWDGKRLRGEEG"
BASE COUNT          516 a          526 c          479 g          535 t
ORIGIN      
        1 ttcttaaaat tttcaatgaa acggcctgcc agttcccttg ctttcagctg gtattcggcc
       61 gggttttccc aactggattc cggattcagg atcttttccg gtaagcccgg acaggtttcc
      121 ggcatggcaa aatgaaatac cggatgcacc ttccaattgc aattccccag ttttccgtcc
      181 aggatagcac tgacgatagc ccgcgtgtag gaaagaggta tccgtgaacc cctgccatag
      241 gcgccgccgc tccatcccgt attgatcagc cagacccgca cctgttcttt acggatcttt
      301 tcacccagca ttttagcata gaagaccgga tgcagcggaa gaaatggcgc tccaaaacag
      361 gcgctgaacg tggatttcgg ttcattgatg ccggcttccg ttccggcaat tttagccgta
      421 tatccggaaa tgaactggta catcgcctgc tcaaccgtca gtcgcgatac cgggggcagc
      481 acaccgaagg catcgcaggt aagaaaaaaa atattggacg ggatcctgcc cagggacggg
      541 accacggagt gcgggatata gtcgatcggg taggacaccc gggtattctc cgtctttgaa
      601 agatcatcat aatttacccg gtctgttccc ggaaaaaatc cggtattctc cagcagggcg
      661 ccagtccgga cggcatgaaa aatatcgggt tcgttttctt ctttcagatg aatgcatttc
      721 gcataacaac ccccctcaaa attgaaaatg ccgtttccat tccacccgtg ttcatcatcc
      781 ccgatcagcc ttctgctccg gtcggcgctc agcgtggttt taccggtacc gctgagtccg
      841 aaaaaaatag cggtatcgcc tgcctttccc atattggccg cacaatgcat ggtcagcacc
      901 cctttctgga aaggaaggag gtagttcagc acggagaaca ccgatttttt tatctctccg
      961 gtatacccgg ttccagccag caggatcagc tggtgccaga atgaaatgat cacggcattc
     1021 tgttgccgga tgccggctga tgccggattc agcttcaggc cgggagcagc caggatatgc
     1081 cagtccgccc tgaatccttc ttcttccgga cgaagaaaca tattataggc aaagagattc
     1141 aatgccgggg tttccgttat cacccgcaga ttaagcctgt atgcgggatc ggcacaaacc
     1201 gaactatccc tcacccatat ttcaggaagc tgattcaggt aagcggtcac ctgccggaag
     1261 atctggtcaa attgtgtttc ttccaggggc tggttgtaat tgttccagtt catattacct
     1321 gcagagatct cgtctttcac caggtaacgg tcttttggac aacgacccgt gaattcgccg
     1381 gtgctgatcc acagtgcacc ggtatcggtc agtttccctt ctttcctgcg taaacactgg
     1441 cttatcagtt cctccgggct taactgataa tggatgcttc cggtaacgtt tagaccgaga
     1501 ttcattaatt ttttcagcgg aagacccatg accggtactg acatatgcaa tcatttgaac
     1561 cccaaaacta aaattaaatt atatctaaaa aaagcctttt ttatacggca taaatttgat
     1621 aatatttatt caaataccct tatatacgat atttattcaa aatactgccg ttaaccggtt
     1681 ttgatagctg aaatatgagg gtttcatttg cctgttgtga tttggccaat cctccatact
     1741 tttacaagga tgaaatacct gccgctcaat cccttgatat ttaagcagaa ccgtcagaga
     1801 ttccaaaagg aaatgttgtc ctcttccatc gctattttcg taagcaacga tgaaatgcct
     1861 tccaatggag acgcgctgta tccttttaag cagaacagcg acctctactg gctcaccgga
     1921 attgtccagg aagagagcat gctgatcctt tttccggatc atcccgatcc gcaattccgg
     1981 gaagtgttgg tcctgatcag gccgaatgaa ctcaaggaga aatgggatgg gaaaagactc
     2041 cgcggggagg agggga
//
LOCUS       DASYCU010000065         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_162615, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000065 DASYCU010000000
VERSION     DASYCU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162615"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VGM24_01285"
     CDS             <1..912
                     /locus_tag="VGM24_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY4154020.1"
                     /translation="PDRVTIAGQSAGSASVNCLLASPLARGLFEAAIAESGSLVLENP
                     LIRMKSLEVAEKQGLELAEKINAKNIQELRALPAEIIQNTLGFYAPIVDGYMMPASVA
                     EIYKQSRQTHVPLLTGWNADEGFLLGIKKKAIFREEAKDFGPDSTIFTKYFPSETDAQ
                     SEASQIALSVDKTIGVPQYAWALKQNENNKAKTFLYRFTRKPPATGDKKRFGAYHTAE
                     IGYALNNLDSIQRPWEPADRKLEEIMSDYWANFVKTGNPNSAQLPDWPAFSDKSAMTM
                     FLGQDPSAKELPDQGALNFLYSKYPGK"
     gene            998..2203
                     /locus_tag="VGM24_01290"
     CDS             998..2203
                     /locus_tag="VGM24_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154021.1"
                     /translation="MMFRRTLTIALQLLLPFFLIAQSSDTQDFEDGGKDVLDGIHRIF
                     HRKNIATDSIFRQRHIRIGILPAMGYTLQTGFAAVLSTNMVIYKRRKQDTILPSTVIA
                     SVSYTQQKQVIFPIQSVIYFKHNRAMLISDWRFLKYPSFTYGLGMHSGPADENQLDFQ
                     YFKFHESILFQLSPGLYAGGGYALDYFWNVKELHPHPSPANDFENYGYRTQTISSGLS
                     FHLLRDTRDNPINAYSGTYSSIAVNPRFKFMGSDASWTTMLLQWRGYFRFPANSANTM
                     ALWSYNWFTLAGKPPYLMLPATGWDKSYNTGRGYIQGRFRSNNMIDGEAEYRIRLTNN
                     GLLGMVLFGNLESFSEINTWRFESVAPAGGLGLRIKLNKYSRTNISIDYGWGKQGSRG
                     FFVNLGEVF"
     gene            complement(2247..>3064)
                     /locus_tag="VGM24_01295"
     CDS             complement(2247..>3064)
                     /locus_tag="VGM24_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4154022.1"
                     /translation="DLANNYGPPPGSAEENFGRIFKQDFKSYRDELIISSKAGWGMWP
                     GPYGDLGSKKYLVASLDQSLKRMGLDYVDIFYHHRPDPDTPLEESMSALDLIVRQGKA
                     LYVGISSYQPEETARAVKILRDLGTPCLIHQPKYSMFDRWIEDGLTDVLKKEGVGCIC
                     FSPLAQGMLTDKYLNGIPEGSRAGSHRGNGAIEESALTTERIGQARKLNEIAKQRGQS
                     LAQMALSWVLRDPAVTSVLIGVSKPAQLTDSIRCLENYSFSAEELKKIDLLLR"
BASE COUNT          787 a          753 c          716 g          808 t
ORIGIN      
        1 cctgaccggg taacgattgc gggtcagtca gccggttctg ccagcgtaaa ctgcctgctc
       61 gcctctcccc ttgccagggg tttgtttgag gctgccattg cggaaagcgg atccctggta
      121 ctggaaaatc ccctgatccg gatgaagtct ttggaggttg ctgaaaaaca gggactggaa
      181 ctggcggaaa agataaatgc aaaaaatatt caggaactga gggcgcttcc ggccgaaatc
      241 attcaaaata ccctgggttt ttacgctccc attgtggatg gctacatgat gccggcttct
      301 gtggcagaaa tttataaaca gtcccggcag acccacgtgc cgttgctgac cggctggaat
      361 gccgatgagg gatttttgtt gggaataaag aagaaagcca tttttagaga agaggcaaaa
      421 gactttggac cggattcgac catttttaca aaatactttc cttcagaaac cgatgcccag
      481 tcagaggctt cccagatcgc tctgagcgtg gataaaacca tcggtgttcc gcaatatgcc
      541 tgggcgctca aacaaaatga aaacaacaaa gcaaagacct ttctgtaccg gtttaccaga
      601 aaaccacccg caaccggaga caaaaaaaga ttcggggctt atcataccgc ggagatcgga
      661 tatgcgttga acaacctgga cagtattcag agaccctggg aaccggccga ccggaaactg
      721 gaagaaatca tgagtgatta ttgggcgaat tttgtaaaaa ccggaaatcc aaatagcgct
      781 caattgccgg actggcctgc gttttccgac aaatctgcaa tgactatgtt cctgggccag
      841 gatccatcgg cgaaagaatt acccgatcag ggtgcactca attttctgta ctcgaaatat
      901 cccgggaaat aataggcaaa ttcctttatc attccgctta acttaaaaat tattttgaat
      961 tgattatctt gggacctgtg actaaccctg accccgaatg atgtttcgaa gaacactgac
     1021 aattgccctg caactgctat tgcctttttt tctaattgca caatcttccg atacccagga
     1081 ttttgaagat ggcggtaaag atgtcctcga tggcattcac cggatttttc atagaaaaaa
     1141 catagccaca gacagtattt tccggcagcg gcatatccgg atcgggatac ttcctgccat
     1201 gggatatacc ctgcaaacgg gatttgcagc ggttttgagt acaaatatgg tgatctataa
     1261 gagaagaaaa caggatacga tcctaccctc cacggtgatt gccagtgttt cctacacaca
     1321 acaaaagcag gtcatatttc ccattcagtc tgtcatttat tttaagcata accgggccat
     1381 gctgatctcc gactggcgtt tcttaaaata tccttccttt acatacgggc tgggtatgca
     1441 ttcgggtccc gctgatgaaa atcaactcga ttttcaatat tttaaattcc atgaatccat
     1501 ccttttccag ctttctccgg gtttgtatgc cggtggcggt tatgccctgg actatttctg
     1561 gaatgtgaaa gaattgcatc cccatccaag tccggcaaat gatttcgaaa actatggata
     1621 tcggacgcaa accatttctt cgggtctttc ctttcatctc ctgagggata cgcgggacaa
     1681 tccaatcaat gcttattcag gaacatattc aagcattgcc gtgaatcccc ggttcaaatt
     1741 tatgggatcg gatgccagtt ggacaaccat gctcctgcag tggcggggtt attttcgttt
     1801 tccggcaaac tcggcaaata caatggccct gtggagttat aactggttca cccttgccgg
     1861 taaaccgccc tacctgatgt tgcccgccac gggctgggac aaatcctaca ataccgggcg
     1921 gggttatatc cagggacgct tcagaagtaa taatatgatc gacggtgaag ccgaataccg
     1981 catccggctg acgaataacg ggctgctggg catggtcttg tttggaaacc ttgagagttt
     2041 ttccgagatc aatacctggc ggtttgaatc cgttgcacct gccggaggac ttggtctgcg
     2101 catcaaactc aacaaatatt cacgcaccaa tatctccatc gattacggat ggggaaaaca
     2161 gggatcgcgg ggattttttg tgaatttggg ggaagtcttt tgaggcttaa gttttaacca
     2221 gcctttattg atcatccacg gttccttcag cgcaacagca aatcaatctt cttcaattct
     2281 tccgcactga aagaataatt ttccagacaa cgaattgaat ccgttaactg tgccggttta
     2341 ctcaccccga ttaaaacgga tgtcactgcc ggatcgcgga gaacccagga aagtgccatc
     2401 tgcgccagac tctgaccgcg ttgttttgcg atctcattga gcttgcgcgc ctgcccgatc
     2461 ctttccgtgg ttagggcgga ttcctcaatg gctccatttc cccggtgact tcccgcgcgg
     2521 gatccttccg gaattccatt gaggtattta tccgtcagca tgccctgcgc caatggagaa
     2581 aagcagatgc aacctactcc ttcttttttc aggacatccg tcaaaccatc ttcgatccat
     2641 ctgtcgaaca tggaatattt aggctggtga ataaggcagg gcgtgcccag gtcacgaagg
     2701 atctttactg cccttgctgt ttcttcgggt tgataactgg aaatgcctac ataaagtgcc
     2761 ttcccctggc gaacgatcag atccagtgca gacatgcttt cttccaatgg cgtatcggga
     2821 tccggccggt gatgatagaa aatgtccaca tagtccaggc ccattctctt cagactttgg
     2881 tccaggctcg ccacgagata ttttttagat cccagatctc cgtagggccc gggccacatg
     2941 ccccatcctg ctttggaaga gatgatcagt tcgtcacggt aacttttaaa atcctgctta
     3001 aaaattcttc cgaaattttc ttcggcggag ccgggtggcg gtccgtaatt atttgccagg
     3061 tcaa
//
LOCUS       DASYCU010000066         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1032108, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000066 DASYCU010000000
VERSION     DASYCU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032108"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /gene="xylA"
                     /locus_tag="VGM24_01300"
     CDS             complement(<1..1228)
                     /gene="xylA"
                     /locus_tag="VGM24_01300"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY4154023.1"
                     /translation="MNIIKGSREFFPGIGQIKFEGRESDNPLAFKWYDENKMIAGKKL
                     KDHVKFAVAYWHSFCNTGGDPFGPGTKRYPWNQSPDAIQAAKDKMDAAFEFITKLGAP
                     YYCFHDTDLVDEGPSSAEYEKRLQTLVDYAKQKQAASGVKLLWGTANVFSNPRYMNGA
                     STNPDFSTVAYAGLQVKNALDATISLGGENYVFWGGREGYMSLLNTDMKREVEHLGRF
                     LGMARDYARKQGFNGIFFIEPKPCEPTKHQYDYDCATVIGFLRQFGLDKDFKLNIEVN
                     HATLAGHTFQHELQVAADAGMLGSIDANRGDYQNGWDTDQFPMQLNDLIESMLVILEA
                     GGLGGGGVNFDAKTRRSSTDPEDLFLAHIGGMDTFARAVIIADNILQHSPYKKFRKER
                     YASFDSGKGKEFEEGKL"
     gene            complement(1238..>1513)
                     /locus_tag="VGM24_01305"
     CDS             complement(1238..>1513)
                     /locus_tag="VGM24_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEY4154024.1"
                     /translation="RVRAGFANMFLSNLFAQTFANLSGCQVELYNTDGSLGAARGAGF
                     GSGYYADLTACFCGMEIVKQLDPHPRERERLREVYEKWKEGLYKLIK"
BASE COUNT          351 a          408 c          341 g          413 t
ORIGIN      
        1 ccagtttacc ctcttcaaat tcctttcctt tgccggaatc gaaagaggca tatctttctt
       61 tcctgaattt tttgtaaggg gaatgctgaa gaatattatc cgcaataatc actgcccggg
      121 caaacgtatc cataccgccg atatgcgcca ggaaaagatc ttccggatcc gttgagcttc
      181 tcctggtttt ggcatcgaag ttcacgccgc cgccacccag tcccccggct tccagaatga
      241 ccagcatgga ttcaatcaaa tcattcagct gcatcgggaa ctgatccgta tcccatccgt
      301 tttgatagtc tccgcggttg gcatcaatgc ttccgagcat accggcatct gccgccacct
      361 gcagttcgtg ctgaaaggta tgtcctgcca gcgtcgcatg attcacttca atatttagtt
      421 tgaaatcttt gtcgaggccg aactgccgga ggaatccgat gaccgtggca caatcataat
      481 cgtactgatg tttggttggt tcacaaggct tgggttctat aaaaaatatt ccgttgaagc
      541 cctgtttcct ggcatagtcg cgcgccatgc cgagaaagcg gccgaggtgt tccacttccc
      601 ttttcatatc cgtgttcagc aaggacatat acccttccct tccgccccag aaaacataat
      661 tctcaccacc cagggagatg gttgcatcca gggcattctt tacctgcagg ccggcatagg
      721 cgactgttga aaaatcggga ttggtggatg cgccgttcat atagcgggga ttggaaaaaa
      781 cattagctgt tccccagagt aatttcacgc cgctggccgc ctgcttctgt tttgcgtaat
      841 ccaccagggt ctgcagtctt ttttcatatt cagcggaaga tggcccctca tccaccaggt
      901 cagtatcgtg aaaacaataa tagggagcgc ccagtttggt gatgaattca aaggcggcat
      961 ccatcttgtc tttcgcagcc tggatcgcat cgggagactg gttccaggga taccgcttgg
     1021 tacccgggcc gaacggatcg cctccggtat tacagaagct gtgccagtag gccaccgcaa
     1081 attttacatg gtcctttaac tttttcccgg cgatcatttt gttttcgtca taccatttaa
     1141 acgcaagcgg attatcagat tcgcgtcctt caaatttgat ctgcccgata cccgggaaga
     1201 attcccggct gcctttgata atattcatga tcagtcttta ttttattaat ttatataatc
     1261 cttctttcca tttttcataa acttcccgga ggcgttccct ttcccttgga tggggatcca
     1321 gctgttttac gatctccatt ccgcaaaaac aggctgttaa atccgcatag tatcccgagc
     1381 cgaaacccgc tcccctggcg gctcccagag aaccatcggt attgtacagt tccacctggc
     1441 aaccactgag attggcaaag gtctgggcaa acaggttact caggaacata ttggcaaatc
     1501 cggcccgaac gcg
//
LOCUS       DASYCU010000067         4071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1468691, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000067 DASYCU010000000
VERSION     DASYCU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468691"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VGM24_01310"
     CDS             complement(<1..328)
                     /locus_tag="VGM24_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY4154025.1"
                     /translation="MVKSMTGFGRAEETVAGKTFLVDVKSLNGKQFELQLKLPGFLKP
                     YEFDIRKLLSEKLGRGSLDCTISLKQTGNAKPVSINTDLAKAYYKALAELSKELNLDP
                     SAILSTL"
     gene            complement(361..957)
                     /locus_tag="VGM24_01315"
     CDS             complement(361..957)
                     /locus_tag="VGM24_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY4154026.1"
                     /translation="MFRYFIIYKPFQVISGFSPESGKTCLKDFFKVPADVYPLGRLDF
                     DSEGLLLLTNDKSLNYRVLHPSFAHEREYWVQVEGDISNRALDQLSEGVPISINGKSY
                     LTRRAVATRFPETPKVPDRNPPIRFRKNIPSSWIKLVLTEGKNRQVRKMTAAAGFPTL
                     RLIRYRISALSLQSMLPGDLIEMNKRECYHKLGLDALI"
     gene            1199..2149
                     /locus_tag="VGM24_01320"
     CDS             1199..2149
                     /locus_tag="VGM24_01320"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY4154027.1"
                     /translation="MKFPAPVSLSWLSQFLGASLIGETQASATGINEIHKVEKGDLVF
                     VDHSKYYASCLQSAASFIIIDQVVECPPGKALLVVAEPFEAYLKIVKHFRPFVGAEQV
                     VSPDAQIGKTSFIYPGAFIGHEVQIGEGCVIHPNVSILDHCIIGNNVIIQAGTVIGSD
                     AFYYNTKKNRAVWYKKMLSCGRVVIADDVEIGAGCTIDRGVTSDTMIGKGSRLDNMVH
                     IGHDTILGRNCLLAAQVAIAGAVEVGDGVILWGQVGVSKTLRIADNVTVLAQSGVGRD
                     LEKGKIYFGSPCEEAAIKKRELVWVKRIPEMWKKIMNTEH"
     gene            complement(2164..3243)
                     /locus_tag="VGM24_01325"
     CDS             complement(2164..3243)
                     /locus_tag="VGM24_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4154028.1"
                     /translation="MKSLLFSCLALILSAAASGQEKAFSIHGKVTDLKTGNPLAGASV
                     FCQNTTTGTISNNDGDFSLRMANGGYDLVISYTGYETQVLRVSNSSKEKDSLVISLKE
                     EDKSLSEVVVTGGGALADGWARYGEFFLENFIGTSNNAAECSILNKDDLLFYYYKKSN
                     RLKVRAKAQLVIINAALGYKIHYQLDSFVNHYDTHISSYTGYPLFEELTGTTDQQTRW
                     KKNRYLAYLGSRLHFMRSWYDSTLNDEGFQIETLDSTNPDKGTLLENPYDSTFYSLDS
                     AVVDIGLRGRIRISYKNRAPDKTYLIKNKFPLSAKIQISALEIPNGFSIEENGYFFEQ
                     SEVINSGYWAWKKLAELVPYDYNPE"
     gene            complement(3414..>4071)
                     /locus_tag="VGM24_01330"
     CDS             complement(3414..>4071)
                     /locus_tag="VGM24_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="3-beta hydroxysteroid dehydrogenase"
                     /protein_id="HEY4154029.1"
                     /translation="YISSVSALGKIVGRETVNEQKKWESPGQLSGYAISKYYAEMEVW
                     RGMGEGLRPLILNPSTILGFGDWNQGSCAIFKTAYNEFPWYANGSNGFVDVEDIARAV
                     TGLMDSPVRDERFIVSSENWSYRQLFDRIADAFNKNHPSKEATPFLAGIAWRTEKIKS
                     IFNGSRPLLTRETAALARKSSFYDNSKLLKALPGFQFTPLEDSIRNACSRYLNNRQPA
                     "
BASE COUNT          996 a          967 c         1018 g         1090 t
ORIGIN      
        1 ccagggtact caggatagcg gaaggatcca ggttaagctc tttggataat tctgcaagcg
       61 ccttataata ggctttggcc agatcggtat tgatggagac cggttttgca tttccggttt
      121 gtttcaggct gatggtgcaa tccagtgatc cgcgtccgag tttttctgaa agcagtttcc
      181 ggatatcgaa ctcgtagggt ttcaggaagc cgggcaattt caattgaagt tcaaactgtt
      241 ttccattgag cgatttcaca tccaccagaa aagttttccc cgccaccgtt tcttccgctc
      301 tgccaaaacc cgtcatcgat tttaccatga taatcctgtt tgtgcctgaa aggtaataaa
      361 ttaaatgagc gcatcgaggc cgagcttatg atagcattcc cttttattca tctcgatcag
      421 atcgccgggt aacatggatt ggaggctcaa cgcggaaatc cggtaacgga tcaggcgaag
      481 ggtgggaaag cctgccgctg cagtcatttt cctgacctgt ctgtttttgc cttcggttag
      541 caccagttta atccaggagc tgggaatatt ttttctgaac cggatcgggg gatttcgatc
      601 cggcaccttg ggtgtttcgg gaaaccgggt ggcgactgcc cttctggtca ggtagctttt
      661 tccattaatg gaaatgggaa cgccctccga caactgatcc aatgcccggt tgctgatatc
      721 cccttccacc tgcacccagt actcccgttc atgcgcaaag gagggatgga ggacccggta
      781 attcagagat ttgtcattgg tcagcaaaag caatccctcg ctgtcgaaat ccaaacgccc
      841 cagggggtaa acatcggccg ggaccttaaa aaaatctttc aggcaggtct tcccgctctc
      901 cgggctgaac ccggaaatta cctgaaacgg tttgtatatg atgaaatatc tgaacaagtg
      961 aattcgatga aactattgcg ggcaaagggt ttcaaaagat cgtaaaaaat aaaaagcccc
     1021 gctggcggcg agacttttac tatttggatg ggttagtaag tacagggaaa taaaattccc
     1081 tacacaatat tagaaataat tttgtcgatc tggaaaaatt ttccaatata ttttatgcac
     1141 aatttttttt cgggtgtgga aaagcacttt aatgtaggtt tacgcaaaat tttctcccat
     1201 gaaatttcct gctccggtat cgttgtcctg gctgtctcaa tttctcggcg cttccctgat
     1261 cggggaaacg caggcatcgg ccaccggcat aaatgagatc cataaagtgg aaaaaggcga
     1321 tctggttttt gtagaccatt ccaagtatta tgcctcctgt cttcagtccg ccgcctcatt
     1381 catcatcatt gatcaggtgg tggaatgccc tcccgggaag gctttactgg tagttgcgga
     1441 acccttcgag gcttatctga aaatagtgaa acactttcgc ccctttgtag gagcggaaca
     1501 ggtagtgagt ccggatgcgc agatcggaaa gacttctttc atctaccccg gcgcctttat
     1561 aggtcatgag gtgcagatcg gggaaggctg tgtgatccat cccaacgtaa gcattctgga
     1621 tcattgtatc attggaaaca acgtgatcat ccaggccggt acggttatcg gctccgatgc
     1681 attttattac aacacgaaaa aaaaccgggc ggtttggtat aagaaaatgc tgagctgcgg
     1741 aagggtcgtc atagcggacg acgtggagat cggggccggc tgcaccatcg accggggggt
     1801 gaccagcgac acgatgatcg ggaagggaag ccggttagac aatatggttc atatcggtca
     1861 tgatacgatc ctgggcagga attgcctgct cgcggcacag gttgccattg ccggcgccgt
     1921 cgaagtgggc gacggcgtga tcttatgggg ccaggtcgga gtgagtaaaa cgctgcgcat
     1981 tgccgataac gtgaccgttc tggcacagag cggcgtaggg cgtgatctgg agaaaggtaa
     2041 aatctatttc ggcagtccct gtgaagaagc cgctataaaa aaacgggagc tcgtttgggt
     2101 taaacggatt ccggagatgt ggaagaagat aatgaacacc gaacactgaa ccggggcgcc
     2161 ccattactcc gggttataat cgtacggaac cagttccgcc agttttttcc aggcccagta
     2221 cccgctattg atcacttcgg attgctcaaa gaaataacca ttctcttcta tcgaaaatcc
     2281 attgggaatc tccagcgccg atatctggat tttggcactg aggggaaatt tgtttttgat
     2341 caggtaggtt ttatccgggg cccggttctt gtagctgatc cggatccttc cccggagacc
     2401 gatgtctact acggcgctgt ccaatgaata aaaagtggaa tcatacggat tttccaggag
     2461 ggtgccttta tccgggtttg tggaatccag ggtttctatt tgaaagcctt catcattcag
     2521 ggtgctgtcg taccagctcc tcataaaatg caggcgcgat ccgagatagg ccaggtagcg
     2581 gttctttttc cagcgcgtct gctgatcggt tgttccggtc agttcttcaa acaacgggta
     2641 accggtataa ctgctgatat gggtatcata atgattcacg aaggaatcca gctgataatg
     2701 aattttataa ccgagcgccg cattgataat caccagttgt gctttggctc tcactttcag
     2761 ccggttgctt tttttataat aataaaagag caggtcatcc ttgttcaaaa tgctgcattc
     2821 tgccgcatta ttgctggttc cgataaaatt ttccaggaaa aattcgccgt atctggccca
     2881 tccgtctgct aatgcgccac ctccggtaac caccacttca gacagactct tatcctcttc
     2941 tttcagggag ataaccaggg agtccttttc cttgctgctg ttgctgaccc gcaatacctg
     3001 ggtttcatat ccggtatagg aaatgacgag gtcgtatcct ccgttggcca tccggagtga
     3061 aaaatcgcca tcattgttgg aaatggtccc ggtggtggta ttctggcaaa acaccgatgc
     3121 gcctgccagc ggattgccgg ttttcagatc cgttacctta ccatggatac tgaatgcttt
     3181 ttcctggccg gatgcggccg ctgaaagtat aagcgcaagg cagctgaaga gaagtgattt
     3241 catccaaaat atttaatctt tttgggagcc ggacggattc ccgcgcccgg tatcctgaaa
     3301 aatagattat gtccgggttc atcaggcagc acaaagtata acgaaatctc ctgtaatgaa
     3361 agtatccgga tattaattta tccggtacga aagaaggtaa agaaaaccgg ctcttatgcc
     3421 ggctgcctgt tattaagata ccgggaacag gcattccgga ttgaatcttc cagtggagta
     3481 aattgaaaac caggtaaggc ctttaacagt ttactgttat cataaaagct gctcttccgc
     3541 gccagggctg ccgtttcccg cgttagcagg ggtctgcttc cattgaagat ggattttatt
     3601 ttttctgttc tccaggcaat tcccgccagg aaaggggtcg cctccttgga cgggtgattc
     3661 ttgttgaatg catccgcgat ccggtcaaac aattggcggt agctccagtt ttccgagctg
     3721 acaataaacc gttcgtcgcg gaccggggaa tccatcagtc ctgttactgc gcgcgcgata
     3781 tcttccacat caacaaatcc gttgctgcca ttggcatacc aggggaattc attataggcc
     3841 gtcttaaaaa ttgcgcagct tccctggttc cagtcgccaa aacccaggat cgtagaagga
     3901 tttaaaatta atggccttag tccctcgccc attccgcgcc acacttccat ctctgcgtag
     3961 tatttgctga tggcatagcc ggaaagctgg cccgggcttt cccatttttt ttgctcattg
     4021 accgtctcgc gacccactat tttccccaaa gcgctcaccg agctgatata g
//
LOCUS       DASYCU010000068         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1032950, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000068 DASYCU010000000
VERSION     DASYCU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032950"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="VGM24_01335"
     CDS             complement(<1..1497)
                     /locus_tag="VGM24_01335"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY4154030.1"
                     /translation="MDLSKHYDPAVVESRWYAHWTARHYFNSFPDERKPFTVVIPPPN
                     VTGVLHMGHTLNETVQDILVRRARMSGFNACWVPGSDHASIATEARVVAMLREKGIDK
                     NSLSREAFLQYAFEWKDLYGGIIYDQIAKLGCSVDWNRTNFTMDDHYYQAVIKVFVDL
                     YRKKLVYRGARMIHWDPAARTALSDEEVEYRDVEGILYYIKYPVAGSADFITIATQRP
                     ETIMGDTGVCVNPDDPRFRQLNGKQVIVPLVNRPVPVIFDDYIDISFGTGALKVTPAH
                     DINDYNLGLKHGLAVIDTLNEDGTLSPAAEILVGMDRFEAREKVVELLREAGLLVKEE
                     KYATRLGYSQRSHAVVEPRISTQWFVKMKPLAEPALEQVLSGNIRIHPGEKFLATYKY
                     WLENVKDWCISRQLWWGQRIPAWYDGKRNFVVAENEQAAMAAYAAEFGNLPAEHLTQD
                     EDVLDTWFSSWLWPIEVFHGITDPGNADINYYYPGNVLVTGQDIIFFWV"
BASE COUNT          419 a          463 c          454 g          410 t
ORIGIN      
        1 cacccagaaa aaaataatat cctgaccggt gaccagcacg tttccgggat agtaatagtt
       61 gatgtccgcg ttgcccggat cagttatccc atggaagact tcgatcggcc aaagccagga
      121 ggaaaaccag gtatccaaca catcctcatc ctgtgtgagg tgctcggcgg gcaggtttcc
      181 gaattctgcc gcataagcgg ccatcgccgc ctgctcattt tcggcaacga caaaattccg
      241 tttgccatca taccaggccg gtatccgttg tccccaccaa agctgacggg aaatacacca
      301 gtcctttaca ttttccagcc agtatttgta agtggcgaga aacttttccc cgggatggat
      361 ccggatattt cccgaaagca cctgctccag ggccggttcg gccagcggtt tcattttgac
      421 aaaccactgg gtggagatcc tgggttcaac cacggcatgg gatctctgtg aatagcccaa
      481 ccgggttgca tatttttcct ctttcaccaa aagtcctgct tcgcggagca gttcaaccac
      541 tttttctctt gcctcgaaac gatccatccc gacaaggatt tccgcggcgg gagaaagggt
      601 gccgtcttcg ttcagggtat cgatcaccgc caggccgtgt tttagtccca ggttataatc
      661 gttaatatca tgggccggcg tcactttaag cgcacccgtt ccaaagctga tgtcgatata
      721 atcgtcaaaa ataacgggca cgggccggtt caccagcgga acaatgacct gcttcccgtt
      781 cagttggcgg aatcggggat catcggggtt tacgcagact ccggtatcgc ccatgatggt
      841 ttccggtcgt tgagtggcta tggtgatgaa atccgcactg ccggccaccg gatattttat
      901 gtagtaaaga atgccctcca cgtcccgata ctccacttct tcatcgctga gggccgtcct
      961 ggcggcggga tcccagtgga tcatccgggc gccccggtaa accagtttct tccggtagag
     1021 gtccacaaaa accttgataa ccgcctgata ataatggtca tccatggtaa aattggtccg
     1081 gttccagtcc acactgcagc ccagtttcgc gatctggtca tagatgatgc caccatacag
     1141 gtccttccat tcgaaagcat attgcagaaa ggcttcgcgg gaaaggctgt ttttgtcgat
     1201 ccctttttcc cggagcatgg ccaccacccg cgcttcggtg gcaatggagg catgatcgct
     1261 tcccggcacc caacaggcat tgaaaccgct catgcgcgcg cgccgcacca ggatgtcctg
     1321 gacggtttca ttgagggtat ggcccatgtg gagcacgccg gttacattgg gcggcggaat
     1381 caccacggta aacggtttcc gttcatccgg aaaactattg aaataatggc gggccgtcca
     1441 gtgggcgtac cacctggatt caacgaccgc gggatcatag tgcttactca ggtccatccg
     1501 ggcatctttt aaaggaatgc aaagttaatc aatagcttat caggatggga gggatggggg
     1561 cacaaaaaaa gtttccttgg gaaaggaaac tttgtcttag gtgagggaat ataaaattcc
     1621 gctcttatcc tgggaataga ggttctgaat taattattca gcaggtcagc ttgtgccggt
     1681 tatgcaaacc agtacaaata ggacagcagc ataacgaggg ccagaaaacc aaaggcgaac
     1741 agggcg
//
LOCUS       DASYCU010000069         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4369074, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000069 DASYCU010000000
VERSION     DASYCU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4369074"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VGM24_01340"
     CDS             <1..930
                     /locus_tag="VGM24_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4154031.1"
                     /translation="KRVVGQDEAIAAVADAIRRSRAGLQDPKRPIGSFIFLGTTGVGK
                     TELAKALAGYLFDDEGMLTRIDMSEYQEKHTVSRLIGAPPGYVGYDEGGQLTEAVRRK
                     PYSVLLLDEIEKANPDVFNILLQVLDDGRLTDNKGRIVNFKNTIIIMTSNMGSQIIQT
                     AFEDISEVNKEEVVEQTRLEVMNLLRQTLRPEFLNRIDEVIMFQPLLKTEIRGIIRIQ
                     LNNLSRMLVKNGIDLRFSDYAVDYLAENGFDPQFGARPLKRLIQKEIVNPLSKKILAG
                     NIHHNSPVLVDVFDGLVVFRNTREEHEKTGATP"
     gene            1127..1696
                     /locus_tag="VGM24_01345"
     CDS             1127..1696
                     /locus_tag="VGM24_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY4154032.1"
                     /translation="MAFKKEIIEIIEPRDVFVGPKTAPVTLVEFGEYENEDCAKAHEV
                     VKKIMEEYEGKVRFNFRHFPLTKIHQRSLKAGEASVAAAQEGKFWEMHNILFHNRHNL
                     GTTSLKLHSREAGISNKKFLDHLVNATYGWQVQGDLREGIQKGVTTVPAFFINGEPFS
                     GKPTFDNLSKGIDAALRKAKKKEKAAVVR"
     gene            1882..>3272
                     /locus_tag="VGM24_01350"
     CDS             1882..>3272
                     /locus_tag="VGM24_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4154033.1"
                     /translation="MKISGTSKGFQEKLKLFSVVRSLARLKEKGYQRFFQGHNKIFSS
                     GMEVLSHKSIQFLVEIKSLGFTKTMDELEKGKLSVFNQLNFFQFLTGIMVPMICFFGN
                     NKFPLTFFLLASLPAWVSLLVLYLNFYYRYEAGMITYFILYPLVTSIVYMSGMNLGVE
                     LYFILNGILAVFFLPLISQMLFCVGLSMVSYFVLVVINNHYNYQLQSANLFLYLFNQI
                     TAILFIFYALFLIKKESSLYQVGILASNKALQETNEKIERQKAEIQEKATELSELNSL
                     KNKLFSVISHDLKTPMYALRNLFGSMQQSNMSGEEIKSIIPDVVTDLNYTTGLMENLL
                     HWVKSQMNAASVYPQPLDLEMITEEVVNVQQLQASSKNISIGCSIPKESTAFADKNMV
                     KLILRNLLSNAIKFTPVNGKIGIRIKPEGVFCKISVKDNGIGMDEASIACIHQNNYYT
                     TNGTAKESGTGLGL"
BASE COUNT          935 a          715 c          799 g          823 t
ORIGIN      
        1 aagcgcgtgg tggggcagga tgaagccata gcggccgtgg cggatgcaat ccgccgcagc
       61 cgggcgggac tccaggatcc caaaagacca atcgggtcat tcattttcct gggaacgacc
      121 ggagtcggaa aaacagaact ggcaaaggcg ctcgcgggct atttattcga tgatgaaggt
      181 atgctgacgc gaattgacat gagcgaatac caggaaaaac atacggtgag ccgtctgatc
      241 ggcgcccctc ccggatatgt cggatacgac gaaggcggcc agctgacaga agccgttcgc
      301 cgtaaaccat acagtgtgct gctcctggac gaaattgaaa aggcaaaccc ggatgtattt
      361 aacatattac ttcaggtgct ggatgacgga aggttgacgg acaataaggg aaggatcgtg
      421 aatttcaaga ataccatcat catcatgaca agtaatatgg gtagccagat catccagacg
      481 gctttcgagg atatttccga agtcaataag gaggaagtcg tggaacagac ccggcttgaa
      541 gtgatgaacc tcctgaggca aaccctgcgc ccggaatttc tgaaccgaat tgatgaggtg
      601 attatgttcc agcccctgct gaaaacagag atccggggaa taatccgcat ccagttgaat
      661 aacctgtccc ggatgcttgt taagaatggg attgacctcc gctttagcga ctacgcggtg
      721 gattacctgg ctgagaacgg attcgaccct cagtttggag cccgcccgtt aaaaagactt
      781 attcagaagg agatcgttaa ccccttgagc aaaaagatct tagctggcaa catccatcat
      841 aattcgccgg tcctggtgga cgtgtttgac gggttggtgg ttttccggaa tacccgggaa
      901 gagcatgaaa aaacgggtgc cacaccttaa taagttgtta actaatttat taaccttccc
      961 ggccacccta aaataagtaa gaatttcggg gaaaattgct atttttagcc gcctctgcca
     1021 atccgtctgg ttaagaggct tttttttcaa cttttggggt ccaaaactgt tatagtaaaa
     1081 gaaaggcaaa aaacgggaag aaaataaata aaaaggaata taaaccatgg cttttaagaa
     1141 agaaatcatc gagatcattg aacccaggga cgtattcgtt ggccctaaaa cagctccggt
     1201 aaccctggtg gagtttggag aatacgaaaa cgaggattgt gccaaggcgc acgaagtggt
     1261 taaaaagatt atggaagaat atgaaggaaa ggtgagattc aatttccgcc attttccgct
     1321 gaccaaaatt caccagcgca gcctgaaagc aggggaagct tccgttgcgg ccgcccagga
     1381 aggcaaattc tgggaaatgc ataatattct attccacaac cgccataacc tgggaaccac
     1441 cagcctcaaa ttacattccc gggaagccgg gatttccaac aagaaatttc tggatcacct
     1501 ggtgaatgcc acttatggct ggcaggtgca gggagacctc cgggaaggaa tccaaaaagg
     1561 ggtaactacc gttccggcct tctttatcaa tggtgaacca tttagtggca aaccgacttt
     1621 tgataacctg agtaaaggaa tcgatgcggc attgaggaag gcgaagaaga aggagaaggc
     1681 tgcggttgtc aggtaaccgg acttaagcag aaaaatcttc aggagcgcca tcggcgctct
     1741 ttttttgtgc cggtccataa ggaaatcccg gcgccgggat tttcccgcgg tcttccggga
     1801 atggccttct ttgtgattat cataattaat tcctaatttg ttcttatccc tggcagcagg
     1861 tttccttaag accaatattg aatgaaaatt tccggaacat caaagggctt tcaggagaaa
     1921 ttgaaattat tctctgttgt ccggtctctc gcacggctaa aggaaaaggg gtaccagcgt
     1981 ttttttcagg gtcacaacaa aattttttcc tccggaatgg aggtgctctc ccataaatca
     2041 attcaattcc tggtcgaaat taaatccctg gggttcacca aaaccatgga tgaacttgaa
     2101 aaagggaaac tgagtgtatt taatcagctc aatttttttc agttccttac cggaattatg
     2161 gttccgatga tttgcttttt cggaaacaat aaatttcccc tgacattttt tctgctggcc
     2221 agcctgcccg cctgggtcag cctgctcgtc ctttacctga acttttatta ccggtatgaa
     2281 gccggaatga tcacttattt tatattatat cccctcgtga cgtcgattgt atatatgagc
     2341 ggaatgaacc tgggggttga actttatttt attctgaacg gaatcctggc cgtatttttt
     2401 ctcccgctca tcagccagat gcttttctgt gtcgggcttt ccatggtcag ttatttcgtg
     2461 ctggtcgtaa taaataatca ttataattac cagctgcaat cagccaacct gttcttatac
     2521 ctgtttaacc agatcaccgc catcctgttt atattctacg cgttgttcct gatcaagaaa
     2581 gaaagcagtc tttatcaggt gggaatactg gccagcaata aggcgttgca ggaaaccaat
     2641 gaaaaaatag aacggcagaa agcagagata caggagaagg ctaccgagct gagcgagctg
     2701 aattccctga agaataaatt gttctccgtg atttctcatg atctcaaaac gcccatgtat
     2761 gcgttacgga atttattcgg cagtatgcag cagtcaaaca tgagcgggga ggagatcaaa
     2821 tcaattattc cggatgtggt cacggatctc aattacacga ccgggcttat ggagaatctg
     2881 ctgcattggg taaaaagcca gatgaatgcg gccagtgtct atccgcagcc cctggatttg
     2941 gaaatgatca ccgaagaagt ggtgaacgtg cagcaattgc aggcttcttc caaaaacatt
     3001 tccattggtt gttccattcc gaaagagagt accgcttttg ctgataagaa tatggtaaaa
     3061 ctgattttga ggaacctgct gtccaatgcc attaagttta ctcctgtaaa tggaaaaatc
     3121 ggcatccgga tcaaaccgga aggggttttt tgcaaaatct ccgtaaagga taacgggatc
     3181 gggatggatg aagcatccat tgcctgcatc catcagaata actattacac taccaatggt
     3241 acggcaaagg aaagcggcac aggccttggc ct
//
LOCUS       DASYCU010000070         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_165007, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000070 DASYCU010000000
VERSION     DASYCU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165007"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..508)
                     /locus_tag="VGM24_01355"
     CDS             complement(98..508)
                     /locus_tag="VGM24_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY4154034.1"
                     /translation="MLSKKTQYAFQALMYLSEKSSDGPVLIAEISKKKKIPLKFLENI
                     LLELKKAGILESKKGKGGGYFFAKDPDSVTLATVMRLIDGPISLLPCVSLYFYEKCWN
                     CDEKHCGLHEVLVQVRDANLRLLEKKTIADLRIK"
     gene            complement(570..1286)
                     /gene="dapB"
                     /locus_tag="VGM24_01360"
     CDS             complement(570..1286)
                     /gene="dapB"
                     /locus_tag="VGM24_01360"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY4154035.1"
                     /translation="MKIALIGYGKMGRAIEKTALSRGHEIALKIDLDNARDLTPENLS
                     GIDAAIEFTGPSSAPANLRECLEAGIPVVCGSTGWLKEYESVRNAFEKKGTPFLYASN
                     FSIGVNIFFELNKKLAALMAAHPEYAVGIRETHHTQKKDAPSGTAITLAEQVMEYIPS
                     LKSWVNQAARSREELSIVSERIDPAPGIHEVVYSSEIDDLEIKHTAHNREGFARGAVL
                     AAEYIHNKKGIFSMKDVLGF"
     gene            complement(1302..2021)
                     /locus_tag="VGM24_01365"
     CDS             complement(1302..2021)
                     /locus_tag="VGM24_01365"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HEY4154036.1"
                     /translation="MRQVFFFLLLLTGSFLNQAAYGQHTDSLPARPATDTILHPVADT
                     VRKKADSIPVRSGILPDSVQHKKDSVTKKKHDPRRATIYSAILPGAGQIYNHKYWKLP
                     IVYAAIGIPVYTFFYNRSWYKKCQYALAVAVSQSQGDSLARVDPQLVNFVKAGDVNDI
                     ITYRDSFRKSQDYSVLYFLLFYGLNIVDATVDAHLRDFNVNSDLSFKVKPMLIPGPNM
                     VAGFSVVFDFHKGRPRSLNMN"
     gene            complement(2031..2951)
                     /locus_tag="VGM24_01370"
     CDS             complement(2031..2951)
                     /locus_tag="VGM24_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY4154037.1"
                     /translation="MTTPNKKDKEALGKGIRSLLQNIDTDLKTPAGGLRPAVAENVTG
                     ILRIPLDLIETNPKQPRRDFEETALQELAASIRIHDIIQPITVSKLAGGKYRIVSGER
                     RYRAGKLAGIKDIPAYIRQANDQQLLELALLENLQREDLNAMEIALSYKRMMDELNFT
                     QEQVSERMGKERSTVANYVRLLKLPPDIQVALRNNIISMGHARALINVDTVDRQLYLF
                     QEIRNRSLSVRQTEELVRKLYKTAPAVKESVKAGLPEGYRKIEDTLATHFSTRVKLSH
                     NNKGSGNISIEYYSVQELNKILDQLGVQIN"
     gene            complement(2975..3775)
                     /locus_tag="VGM24_01375"
     CDS             complement(2975..3775)
                     /locus_tag="VGM24_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4154038.1"
                     /translation="MARIIGVANQKGGVGKTTSAINLAASLAVLEFRTLLVDADPQAN
                     STTGIGFDLHNITQSLYDCMVNQTAARDVVLKSEVPHLDLIPSHIDLVGAEIEMINYP
                     ERESVLKNILEPIRGSYDFIIIDCSPSLGLITVNALTASDSVIVPVQTEFFALEGLGK
                     LLNTVKIVQSRLNPNLAIEGILMTMYDGRLRLCNQVVSEVRKHFDEIVFNTIIHRNTK
                     ISEAPSVGKPVILYDAYSKGAMNYLNLAKEVLQKNNMTKMTQEERILE"
     gene            3952..4449
                     /locus_tag="VGM24_01380"
     CDS             3952..4449
                     /locus_tag="VGM24_01380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY4154039.1"
                     /translation="MYLIISGTNRKGSYTLRIAELYRQLLKGKNIEAPILSLVDLNVL
                     ERNSSILGLEQELLIPAEKIVFISPEYNGSIPGVLKCLIDVTDSRKVWGGKKALLTGI
                     SRGRAGNLRGMDHLTGILHYVSMVVHPNKLPISIVDTLFDQNGMGHAETLRAINNQLD
                     EFIAF"
BASE COUNT         1043 a         1175 c         1161 g         1264 t
ORIGIN      
        1 ggtcctggaa tgggcggaat ggattattaa ttcagccatt acttataaaa aaaaccctct
       61 tccaaagaag agggtttttt ttgcgggtat actgttttca ttttattctc aggtctgcaa
      121 tcgttttctt ttccagcaac ctcagattcg cgtcgcgcac ctgcaccagc acctcatgca
      181 ggccgcagtg tttttcatcg cagttccagc atttttcgta gaaatagagg ctcacgcagg
      241 ggagcaggga aatgggtccg tcaatcagcc gcatgaccgt ggccagggtg accgaatcgg
      301 ggtctttggc gaaaaaataa ccgcctcctt ttcctttctt gctttccagg atgccggctt
      361 tctttaattc caggagaata ttttcgagga atttcaacgg gatctttttc tttttggaga
      421 tttcggcaat aagaacgggc ccgtcactgg atttctccga caggtacatc agcgcctgga
      481 atgcgtattg ggtttttttg gaaagcatac gacggtttga ttacaagtta tattttatta
      541 ataacagttt ggccgccgga cagttctttt taaaatccca gcacatcctt catggaaaaa
      601 atcccctttt tgttatggat atattcggct gccagaacgg cgccgcgggc aaacccttcc
      661 cggttatgtg ccgtatgttt tatttccagg tcgtctatct ccgaagagta aaccacctca
      721 tgaattcccg gagcgggatc gatccgttca ctgacaatgg aaagttcttc ccgggaacgc
      781 gccgcctgat tcacccagga tttcagggaa ggaatgtatt ccattacctg ctcggccagc
      841 gtaatggccg ttccgctggg cgcatctttt ttttgggtgt ggtgcgtttc ccggatcccc
      901 acggcatatt ccggatgcgc cgccatcagg gcggccagtt ttttattcag ttcaaagaaa
      961 atattcacgc cgatactgaa attgctggca tacaaaaaag gagttccttt tttttcgaac
     1021 gcatttctga cggattcgta ttcttttaac cagccggtgg atccgcagac cacgggaatg
     1081 ccggcttcaa ggcattcccg gagattggcc ggcgcactgg aagggccggt gaactcaatg
     1141 gccgcatcga tcccggacag gttctccggg gttagatcgc gggcgttatc caggtcgatt
     1201 ttcaaagcga tctcatggcc cctggacaag gctgtttttt ctattgcccg gcccattttt
     1261 ccatatccga ttagtgcaat cttcataagg gatcaaagat attaattcat gttcagtgat
     1321 ctcggccttc ctttatggaa gtcaaagacc acgctgaagc cggccaccat attggggccc
     1381 gggatcagca tcggcttcac cttaaagctc aggtcgctgt tcacattgaa atcccgcaaa
     1441 tgcgcgtcca ccgtggcgtc cacaatattc agcccgtaaa acaacaggaa gtacaggacg
     1501 gaataatcct ggcttttccg gaaactgtcg cggtaggtga tgatatcgtt cacatccccc
     1561 gctttaacaa aattgaccag ctggggatcc acccgggcga ggctgtcgcc ctggctttgg
     1621 ctgacggcaa ccgccagcgc gtactggcat ttcttatacc agctcctgtt gtaaaaaaaa
     1681 gtataaaccg gtatcccgat ggccgcgtaa acgatgggca gtttccagta tttatggtta
     1741 tatatctggc cggcgccggg aaggatcgcg gaatagatcg tggcccggcg cgggtcatgt
     1801 ttcttcttcg tcaccgaatc ttttttatgc tgaacgctat cgggaagaat gccgctcctg
     1861 accgggatgc tgtcggcttt tttcctcacc gtatcggcca cgggatgaag gatggtatcg
     1921 gtggcgggac gggcgggcag gctgtcggta tgctgaccgt aagcagcctg gttaagaaac
     1981 gatccagtca ataaaaggag aaagaaaaag acctgcctca taccgaacgc ttaattgatc
     2041 tgcacaccca gctgatccag gatcttattg agttcctgaa cggaataata ttctatggaa
     2101 atattgccgg accctttgtt attgtgactg agctttacgc gggtactgaa atgtgttgct
     2161 aaagtatctt caatttttct gtatccttcc ggcagtccgg cttttacaga ttctttaaca
     2221 gcgggtgctg tcttgtataa tttcctcacc agctcttcgg tctgccgcac cgagagggag
     2281 cggttccgga tctcctggaa gagatagagc tgcctgtcca cggtatccac attgatcagt
     2341 gctctggcat gacccatgga gatgatatta tttctgagcg ccacctggat atccggcggc
     2401 aatttcagca ggcgcacata attggcgacg gtgcttcttt ccttgcccat tctttcggat
     2461 acctgttcct gcgtgaagtt cagttcgtcc atcatgcgct tgtagctgag agcgatctcc
     2521 atggcgttga gatcctcgcg ctgcaggttt tccaggaggg caagctcgag cagttgctga
     2581 tcattggcct gccggatata agcggggata tctttaatgc cggccagctt cccggcgcgg
     2641 taccgccgct cccctgaaac gatccggtat ttaccaccag ccagcttcga aacggtgatg
     2701 ggttgaatga tatcgtgtat ccggatggaa gcggcaagct cctgcagagc ggtctcctca
     2761 aaatcgcggc ggggctgttt tggattcgtt tcgatcaggt cgagcggaat gcgcaggatg
     2821 ccggtcacgt tttcggccac ggccgggcgc aggcctccgg caggcgtttt caggtcggtg
     2881 tcaatattct gaagcagaga gcgaattcct tttcccaggg cttctttatc ttttttattc
     2941 ggcgtggtca tgtttgattt gtattaccat tcagctattc caggatgcgt tcttcctgcg
     3001 tcattttggt catattgttt ttctgcagca cttctttcgc caggttgagg tagttcatgg
     3061 cccctttgct gtacgcatcg tacaggatca ccggttttcc cacgctgggc gcttcgctga
     3121 ttttggtgtt gcggtgaatg atggtattaa aaacgatttc gtcaaaatgt ttccttactt
     3181 cgctcaccac ctggttgcac agccgaaggc ggccatcata catcgtcatg aggatccctt
     3241 cgatggccag gtttggatta agccggctct gcacgatctt gacggtattc agcagcttac
     3301 ccaatccttc cagggcaaaa aattccgttt gcaccggcac gatcacggaa tccgaggccg
     3361 tgagcgcatt gaccgtgatc aatcccaggg aaggggagca gtcgatgatg ataaaatcat
     3421 agctgccgcg gatgggttcc aaaatatttt taagaacgct ttcccgttcc ggataattga
     3481 tcatttctat ttccgcaccc accaggtcga tatgggaggg gatcagatcc aggtgcggga
     3541 cctccgattt caggacgaca tcccgggcgg cggtctgatt caccatgcaa tcgtacaggc
     3601 tctgggtgat attatggagg tcgaagccga tcccggtcgt gctattggcc tggggatctg
     3661 catccaccag cagggtcctg aattcaagca ccgcaaggct tgccgctaaa ttgatggcgg
     3721 aagtggtttt tccgacccct cctttctggt tcgccacccc gataattctt gccatacgcc
     3781 cttcagtttt aaaaatgggt gcctggccgc aaaaacggca aagctcgtaa agatgtatcc
     3841 tatcgggaaa agtacaaaat tttgtttgcc ttcccggctc tttctcctgt aaaaatacaa
     3901 acttgcccgg cgacttgaga aaaatacccg ttccccctaa cttgcacttc catgtacctc
     3961 atcatatccg gcacgaaccg caaggggagt tatacccttc ggattgcaga attgtatcgg
     4021 cagcttttga agggaaaaaa tattgaagcg cccattcttt ccctggtgga tctgaatgtg
     4081 ctggaaagaa attcttccat cctcgggctg gaacaggaat tgctcattcc cgctgaaaag
     4141 atagtattca tcagtcccga gtacaatgga agtatcccgg gcgtgctgaa atgcctgata
     4201 gatgttacgg actcccggaa ggtctggggc ggaaaaaaag ccctgctgac cggcatctcc
     4261 aggggaaggg cgggaaacct ccggggaatg gaccacctca ccggcatttt gcattatgtg
     4321 agtatggtgg ttcatccgaa caaactgcct atttccattg ttgacaccct gttcgaccaa
     4381 aacggaatgg gtcatgcgga aacactgagg gcaatcaaca accaactgga cgaattcatt
     4441 gctttttaaa tattcagata aattcctttc atgcgcaaca caagttttat ctggctggtg
     4501 gccctcgcta tggcgctgct cgacctctac gtttttcaga ttgtaaaatt actttcgcag
     4561 agcttttccc accggtcaaa gaccattctt ttcaccacct actggaccat ttccattgca
     4621 gttgtcattt tattcctgct cct
//
LOCUS       DASYCU010000071         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4369267, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000071 DASYCU010000000
VERSION     DASYCU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4369267"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..971
                     /locus_tag="VGM24_01385"
     CDS             222..971
                     /locus_tag="VGM24_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY4154040.1"
                     /translation="MAIFKSGNPALNVFQNTITIPGSGTMTEKGTLNKFFLLFLLVMG
                     SASLTWKAFYDGVNVMPWIMGSAILGFIVALVVVFKKEWASFLTPVYGLLEGVFVGGI
                     SAVYNNVFAKTAPGIVTQAVLLTFGAVIAMYLLYRFRVIRATEKFRSVIFIATAGIAI
                     FYVLSMVLRMFGVDIAFIHEGSTLGIAFSLFVVAIAALNLILDFDMIEKGVAAGAPKY
                     MEWYGAFGLLVTIVWLYLEILRLLAKLSSRR"
     gene            complement(1035..1832)
                     /locus_tag="VGM24_01390"
     CDS             complement(1035..1832)
                     /locus_tag="VGM24_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEY4154041.1"
                     /translation="MNNHEIDYKIYGEELQFVEVELDPQETVIGEPGGFMMMDDGIEM
                     QTILGDGSPQQGSGLLGKLLNAGKRVLVGENLFMTAFTNTGQGKKRVSFAAPYTGKII
                     PLDLQLLGGTLIAQKDAFLCAAKGVSVGIAFQRRLGTGIFGGEGFIMEKLEGDGLAFV
                     HAGGYVMERELKAGEILKVDTGCVVAYTPGIDFDIQFIRGVRNWMFGGEGLFFALLRG
                     PGKVWLQSLPISRLASRIIQYSPQLGSRKEEGSILRGLGNLMDGDGF"
     gene            complement(1859..>3387)
                     /locus_tag="VGM24_01395"
     CDS             complement(1859..>3387)
                     /locus_tag="VGM24_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4154042.1"
                     /translation="VAIGASDPQSGDRSTRPELGRIQVSFLEFEKRHGFHTSVYLDSV
                     RRVLKGIPGATISVDQEASGPPTDPQINIEISSDNFDDLTKTAEGLKNYLDSLQTPGV
                     EELKMDVDLTNPEITLTVDRQRAMAEGVNSATIGQQIRTALFGKEVSKIKENKEEYKI
                     QLRNNEVQRQNLSDLLNMKVVFREPTGAIKQIPIVNLVKIDYTSTYGSIKRKSLKRVI
                     TLYSNVLNGYTPTAVNAQLKQAIDNFTHKADDVTITQTGEGKQQQETGAFLFKALIYA
                     LMTILIILVIQFNSISKPVIILTEILFSVIGVLLGFGFTGMTISVVMTGLGIVGLAGI
                     VVKNGILVIEFADVLRSRGLKTREALIQAGKTRIIPVMLTALAAILALIPLAIGFNIN
                     FISLFAELNPHIFFGGDSAVFWKPLSWTIIFGLAFAFFMTLIILPSMYLIAERLKRPM
                     RRYFGGKWISMMGIPPLTFIFLLILIPVTIVRHFIEKARRRRRLAGKGVSETWIGSWF
                     "
BASE COUNT          812 a          885 c          778 g          912 t
ORIGIN      
        1 gccgccccgc aacaaataag aggccgtgat ccggcagaag aaaagcggag agacccgttc
       61 ctggcttaga cagcctggat cccgggagaa aatctttccg gacgcgattc ccggaaatcc
      121 gtgccgtttt gttaaaattg accgaagtgc tttttctgct ttccgcctct ttacaatgcc
      181 tttgcttttt cgtaaattgc attccataaa cgaatcaaac tatggctatt tttaaatctg
      241 gcaatccggc actcaatgtg ttccagaata cgatcacaat acccggttcg ggtaccatga
      301 cggaaaaagg aacgcttaat aagttcttcc tgttgttttt actggtgatg ggttctgctt
      361 cattgacctg gaaagctttt tacgatggcg taaatgttat gccctggatc atgggatcgg
      421 ccatcctggg attcatcgtc gccctggtgg tggtcttcaa aaaagaatgg gcttcttttc
      481 taactccggt ctatggatta ctggaaggcg tttttgtggg cgggatttct gccgtataca
      541 ataatgtatt cgcaaaaacg gcaccgggca tcgtaaccca ggccgtgctg ctgacatttg
      601 gcgcggtgat cgcgatgtat ttgttatacc gtttccgggt aatccgggct actgaaaagt
      661 tcaggtccgt catttttatt gcaacggccg gcatcgcgat cttttatgtg ttatccatgg
      721 tgctgcgcat gttcggggtc gacattgcat ttattcatga aggttccacc ctgggcattg
      781 ctttctccct gttcgtggtt gccattgcgg cattaaacct gatcctggat tttgacatga
      841 ttgaaaaagg agttgctgca ggcgctccga aatatatgga atggtatggc gctttcggcc
      901 tgctcgtcac catcgtctgg ctatatctgg aaatccttcg cttactggcc aagctttcca
      961 gcagaagata attattgtta aacgcagtga agccgcggaa tttgaatagg attcaaactt
     1021 ccgtggcttt ttctttagaa tccgtctccg tccatcaggt taccaagacc tcttaaaata
     1081 cttccttcct ccttccgcga tcccagctgc ggactgtatt gaatgatccg gctggccaaa
     1141 cggctgatgg gtaatgattg caaccagacc tttccgggtc cgcgcaacaa ggcaaaaaat
     1201 aacccttcgc cgccaaacat ccagttgcgg acgccccgta tgaattgaat atcgaaatct
     1261 attcccggag tataagccac gacacagcct gtatccacct taagtatttc gcccgctttc
     1321 aattctcttt ccatcacgta tcccccggca tgaacaaagg caagcccgtc tccttcaagt
     1381 ttctccataa taaacccctc tccgccgaat atcccggttc ccagtcttct ttgaaaagca
     1441 atacccacac tcacgccctt tgcggcacaa agaaaggcat ccttctgtgc aattaaggtc
     1501 ccgcccagca actgaagatc caaaggaata attttacccg tatatggtgc ggcaaaactg
     1561 accctttttt taccctggcc ggtattggta aatgcggtca tgaacaggtt ttcgccgacg
     1621 agcacccgtt tgccggcgtt gagtaatttg cctagaagcc cggaaccctg ttgcggggaa
     1681 ccgtctccca gaatggtttg catttctatc ccatcatcca tcatcataaa accgcccggt
     1741 tcaccgatca ccgtctcctg cgggtccagt tccacctcca caaactgcag ttcttcccca
     1801 tatattttat aatctatttc atgattgttc ataaaaaaaa tttagacaaa attaagtatc
     1861 agaaccagga tccgatccag gtctcgctca ctccttttcc tgccagccgt cttcttcgcc
     1921 tggctttttc aatgaaatga cgcacgatgg tcaccgggat caggatcagc aggaaaataa
     1981 atgtcagcgg cgggatgccc atcatagaga tccattttcc gccaaaatac cggcgcatgg
     2041 ggcgttttag cctttccgca atcaggtaca tactgggcag gatgatcagc gtcataaaaa
     2101 aggcgaaggc cagcccgaaa ataatggtcc aggataaggg cttccagaat acggcactgt
     2161 caccaccaaa aaaaatgtgc ggattgagtt cggcaaacag ggaaatgaaa ttgatgttga
     2221 atccgatggc cagcgggatc agcgccagga tggccgccaa agctgtcagc ataaccggga
     2281 tgatccgggt tttgcctgcc tgtatcaggg cttccctggt tttcaggcca cggctcctca
     2341 gtacatccgc aaattcaatc accaggattc cgtttttaac cacgatgcct gccagaccaa
     2401 caataccaag tcccgtcatc accaccgaaa tcgtcatgcc ggtaaatcca aacccgagta
     2461 aaacaccgat cacgctgaac aggatttccg tcagaatgat caccggttta ctgatggaat
     2521 taaactggat aacgagaatg atcaaaatgg tcattaacgc gtatatgagc gccttgaaaa
     2581 gaaatgcgcc ggtctcctgt tgctgtttgc cttctccggt ttgagttatc gtaacatcgt
     2641 ctgccttgtg ggtgaaatta tcgatggcct gtttcagctg ggcgttcact gccgttggcg
     2701 tatacccgtt cagcacattg gagtaaagcg tgattacgcg cttgagggac tttcgcttga
     2761 tactgccata ggtactggta taatctattt ttaccagatt gacgatcggg atctgcttga
     2821 tggccccggt aggttcccgg aaaaccactt tcatattcag caggtcagac aaattttgcc
     2881 gctgcacctc attattacgc aactgaatct tgtattcttc tttattctct ttgatcttcg
     2941 aaacttcctt accaaaaagg gctgtccgga tctgctgacc gatggtagcg ctgttgacgc
     3001 cttccgccat cgcccgctga cggtctacgg tgagcgtgat ttcgggattc gtcaggtcca
     3061 catccatctt gagctcttcc actccgggcg tctgaaggga atccaggtaa tttttcagtc
     3121 cttcggccgt tttggtcaga tcatcaaaat tgtcgctgct gatctcgata ttaatctggg
     3181 gatcggtggg cggcccgctg gcctcctggt caaccgatat ggttgctccc ggaataccct
     3241 tgagtacacg ccgtacggaa tcgaggtaaa cagaggtgtg aaatccatgg cgtttctcaa
     3301 attccagaaa actgacctgt atccgcccaa gttccggacg cgtgctccgg tcgccgcttt
     3361 gcggatcact cgcgccaatg gccacat
//
LOCUS       DASYCU010000072         3034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4078256, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000072 DASYCU010000000
VERSION     DASYCU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4078256"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="VGM24_01400"
     CDS             complement(<1..1698)
                     /locus_tag="VGM24_01400"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154043.1"
                     /translation="MNKKIFQYLPLLFICLTLRAQGPPVFTGSTAARARSTAMVSKYL
                     SPVRIVWQSAHDSSILNAELLLLPGHGQADLTGSRMCELKSTKTNHPALLLDFGMELQ
                     GGLQLITGALPGHLPLNLRVRFGESASEAMSDIEPEHNATNDHAVRDEILQVPMYGGR
                     ETGNTGFRFVRIDLLDDSAELRLKEVSAIFVYRDIPYLGSFHCSDTLLDKIWMTGAYT
                     VHLNMQDYLWDGIKRDRLVWIGDMHPEISTICAVFGSNEVVPKSLDLARDITPLPKYM
                     SGISSYSMWWVIIQRDWYLHTGDLNYLQRQKTYLSALLRLLAEKINSDNSEDLNGRRF
                     LDWPSSEDPAAIHAGLQALMILCMNSGSYLCSVLNDQATERICKNAVEKLRKNIPDVK
                     NSKQAAALLALAGLMPARRANEIIAEDGAHRFSTFYGYYMLQAKARANDYEGALQNIR
                     EYWGAMLRMGATTFWEDFNLDWLPNASRIDELPGPGQKDIHGDFGAYCYIGFRRSLCH
                     GWSSGPTPWLTEHVLGIKVIKPGCREIKIEPHLGDLRFAEGSFPTPFGLVKVKHVRQS
                     "
     gene            complement(1728..2822)
                     /locus_tag="VGM24_01405"
     CDS             complement(1728..2822)
                     /locus_tag="VGM24_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY4154044.1"
                     /translation="MIRKIQICAGLFLLLLTFGANAQQRGSITHSDAPGKVWSAEKAN
                     AWYNRHKWLTGADYIPATAVNQLEMWQADSFDPEEIDRELGWAEAIGFNTMRVFLHSI
                     AWKEDPEGFKKRIDRFLSITDKHHIQPMFVFFDDCWNKQPRPGPQPAPKPGVHNSGWV
                     QDPGQPASENPANFPALEKYVKDVLRRFAHDRRILLWDLYNEPGNSGKNEASLPLLKK
                     IFEWARSVNPDQPLTAGLWDWNFEKLNAFQVANSDIITYHDYEEPAWHQRVISLLKAF
                     GKPLICTEYMARPRNSRFSNILPMLKARHVGAINWGFVSGKTNTIYAWDKPIPDGSEP
                     VEWFHDIFRKDGTAYKQDEVDLIKKLNGIQ"
BASE COUNT          709 a          797 c          747 g          781 t
ORIGIN      
        1 tgactgcctc acgtgtttta cttttaccag accgaaaggc gttggaaaac ttccttcggc
       61 aaaacggaga tcgcccagat gtggttctat tttaatttcc ctgcaacccg gttttatcac
      121 ttttattccc agcacatgtt cggtaagcca gggagttgga ccggacgacc agccatggca
      181 gagactgcgc cggaaaccaa tatagcagta agctccaaaa tcgccgtgga tatctttttg
      241 tcccggcccg ggcaactcat caatacggga cgcgttgggt agccagtcca gattaaaatc
      301 ttcccagaaa gtagtggcgc ccatcctcag catggcgccc cagtattccc ggatattctg
      361 gagggcgcct tcgtaatcat tcgccctggc ttttgcctgg agcatataat agccataaaa
      421 cgtcgaaaac cgatgtgccc catcctctgc tattatctca ttggctcggc gggcaggcat
      481 cagtccggcc agcgcgagta aagcagccgc ctgttttgaa ttttttacgt ccggaatatt
      541 tttacgaagt ttttcaacgg cgtttttaca gatccgctcc gtagcctgat cattcagcac
      601 agagcacaaa taagaacctg agttcatgca caggatcatc agggcctgca atccggcatg
      661 tatggctgcc ggatcttcac ttgaaggcca gtccaggaac cggcggccgt ttagatcttc
      721 gctgttatcg gaatttatct tttcagccag taaccttaac agagcggata aataggtttt
      781 ctgccgttgc aggtaattca gatcaccggt gtgcaggtac cagtcccgct gaataatcac
      841 ccaccacata gaataggagg atatgccact catgtatttt ggtaatgggg taatatcccg
      901 ggcaaggtca aggctttttg ggaccacctc attgcttcca aacacggcgc agatcgtgga
      961 aatttcagga tgcatatccc cgatccaaac caggcggtcc cgcttgatgc cgtcccacag
     1021 ataatcctgc atatttagat gaaccgtata cgcgccggtc atccagatct tgtcgagcag
     1081 cgtatcgctg caatgaaagg aaccgagata aggaatatcg cggtatacaa aaatcgcgct
     1141 cacttctttc aaccgcaact cggcagaatc atccagcaaa tcgattctta caaaccgaaa
     1201 gccggtatta ccggtttccc gtccgccata catgggcacc tgcaggattt cgtcccgcac
     1261 ggcatggtcg tttgtggcgt tgtgttcggg ttctatatcg ctcattgctt cactggccga
     1321 ttcaccaaag cggacgcgca ggttcagcgg caggtgcccc ggcaacgctc cggtaatcaa
     1381 ttgcaggccc ccctgcaatt ccatcccaaa atcgagcagc agggccgggt gatttgtttt
     1441 tgtgcttttc agctcgcaca ttcttgaacc ggtcagatcg gcctgcccat ggcccggcag
     1501 cagcaggagc tcggcattta agatactgct gtcgtgagca gactgccata caattcttac
     1561 gggagataaa tatttgctga ccattgcggt gctccttgcg cgggccgctg tgctgccggt
     1621 aaaaaccgga ggcccctggg ctctcaaagt cagacaaata aacaggagcg gcaggtactg
     1681 aaatattttt ttattcatcg gccggtgttg aaaaacggaa tatccggtta ttgaatgccg
     1741 tttaattttt tgatcaggtc cacctcatcc tgtttgtagg cggttccgtc ttttctgaat
     1801 atatcatgga accattcaac aggctcactg ccgtccggaa tgggtttgtc ccaggcataa
     1861 atggtatttg ttttaccact tacaaaaccc cagttgattg ctccgacatg cctggctttc
     1921 aacatgggca gaatgttgga aaaacggctg tttctgggac gggccatata ttcggtacag
     1981 ataagcggct ttccgaaagc ctttaaaagg gaaatgaccc gttggtgcca ggccggttcc
     2041 tcataatcgt ggtaggtaat gatatccgag ttagccacct gaaacgcatt cagcttttcg
     2101 aaattccagt cccataatcc cgcggtcagc ggctgatcgg gatttacgga tcttgcccac
     2161 tcaaatattt tcttcagtaa aggaagagag gcttcgtttt ttccactgtt tcccggctca
     2221 ttgtacaggt cccatagtaa aatgcgcctg tcgtgcgcaa agcggcggag gacatccttc
     2281 acatattttt ccagcgcggg aaagttagcc gggttttcgg aagcaggctg gcccggatcc
     2341 tgcacccagc ctgaattgtg aactcccggt ttgggtgccg gctggggtcc gggccggggc
     2401 tgtttattcc agcaatcatc aaaaaatacg aacatgggct ggatatgatg tttatccgta
     2461 atggataaaa accggtctat tcttttctta aacccttccg gatcctcttt ccaggcaatc
     2521 gaatgcagaa aaacgcgcat cgtattaaat ccgattgcct ctgcccatcc gagctccctg
     2581 tcaatttctt ccggatcgaa gctgtctgcc tgccacatct cgagctggtt gaccgcagtg
     2641 gccggaatat aatcggcccc ggttaaccat ttgtgccggt tataccaggc atttgctttt
     2701 tcagcggacc atactttccc gggcgcgtcc gaatgggtga tgctgccccg ctgctgtgca
     2761 ttggcaccga aggtgagaag aagcaggaat aatccggcac agatctgaat ttttcgaatc
     2821 attggctgat catttatttt taaaattaaa tggcaatcgg tctaacccgc cggagcggga
     2881 atggctatgc ctgctctcac cggcaatcca aagtccggct ctccgccggc atcccaggta
     2941 aatggctggg cgcgcggcga ccgggagttg ccacaaccct gatccggttt tgaattggca
     3001 tggtatagaa tccagtcttc ttttccatcg ggcg
//
LOCUS       DASYCU010000073         4941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1904961, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000073 DASYCU010000000
VERSION     DASYCU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904961"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..604
                     /locus_tag="VGM24_01410"
     CDS             17..604
                     /locus_tag="VGM24_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY4154045.1"
                     /translation="MENILDRGGEAFYFPEFFLAEESDRYFQELLREIAWNQEPVKIF
                     GKMLMQPRLTAWYGDPGKPYRYSGITMHPKNWTTPLLEIKQRIEKRSPALFNSALLNL
                     YRDQKDHVGWHRDNEKSLGAQPVIGSVSFGATRRFLFRYYADKKIKRPLELTHGSFLL
                     MQGQTQHFWEHCIPKQALAAGPRINITFRNIQEPV"
     gene            complement(589..1575)
                     /locus_tag="VGM24_01415"
     CDS             complement(589..1575)
                     /locus_tag="VGM24_01415"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY4154046.1"
                     /translation="MLHGKELILATRPYAKEVRVKSWFHTLSTIFFLALAFAGVIFIP
                     YLAVRIVFSIISGLLLVRMFIIYHDHQHHSILTHSKIANFIMTAFGFYILSPTSIWKR
                     SHDHHHNHNSKLFSASIGSYPIVSRKKFQEMSSRDKFAYLAIRHPLTIGLGYFTMFIF
                     GMCIASFASNPRKHYDSLIALVLHVTASILIFVFFGWQMWFCLFFLPFIVAFGLGAYL
                     FYAQHNFPGVTFAENNEWAYEKAAMESSSYMVMNPVMAWLTGNIGYHHIHHLNSKIPF
                     YRLPEVMRDFKELQNCKRTSLSPRAVIACLRLKIWDAEKHQMVGLRDLHTGS"
     gene            complement(1586..2464)
                     /locus_tag="VGM24_01420"
     CDS             complement(1586..2464)
                     /locus_tag="VGM24_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4154047.1"
                     /translation="MSAILYYLALPFIYLLSILPFPLLYGLSNIVYYLLFHVLGYRRE
                     VVLNNLRNSFPEKTEKELLLIRRRFYRYFCDLFLETFKTLSISRERMLKHCRMEEESQ
                     NLFKELAAAGQSIMIVMGHLGNWEWAGNTFSLLCKHQLYVIYHPLSNKYFNRLVYKMR
                     TRFGTKLISMKDTLREMLNNRNELNATAFIADQSPQPDRAYWMSFLNQDTPVFLGIEK
                     IALKIRYPVVYVSIERVKRGYYRLHAERIELPLAESRPGEITEFHTRKLEEDIRRQPE
                     IWLWTHRRWKHTRKTS"
     gene            2597..3334
                     /locus_tag="VGM24_01425"
     CDS             2597..3334
                     /locus_tag="VGM24_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154048.1"
                     /translation="MKLKPGLVFLISLTALIFSGCNKKNPLKTDIPADYYPIQVGKYI
                     IYRMDSLKFINIGSQDTTISYLAKEVVEDSLTDNLGRPSYRVVRYLSDTTGTQPWEPS
                     VAYLVTPTSQSVEVVDNNLRFIKLVTPVQDYISWKGNVYIDTKSVNSQVPYMDDWNYT
                     YANTGQPYDVLEGTIPETVIVNEENETSGIEGSYSQTIISVEVYGKGIGPIYKNFLHS
                     EYQAPNLSNPAGTTIGYGLTFNMVSHN"
     gene            complement(3363..4550)
                     /locus_tag="VGM24_01430"
     CDS             complement(3363..4550)
                     /locus_tag="VGM24_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fmu (Sun) domain-containing protein"
                     /protein_id="HEY4154049.1"
                     /translation="MMFDNLLRYAVRIVDAYSGEKPLHVWLKEFFRANPQMGSRDRKL
                     VSEMVYCFYRLGRSLPEAGSGERILAGMFLCNQRGMQALEFLAPDWNAAIEMPVEEKV
                     QILKKKFPGFSIDQIFPFKDLLSAGVDPAAYCLSFLKKPELFIRIRKGKEAVVRKKLV
                     ENKIAFQELAPDDRLIFTACSFPNGTRLDGVLDVNREAVVQDLSSQKTAGFLKMPDAD
                     PEKIIRAWDCCAASGGKSILLADLYPTLDLTVSDIRKSILENLRTRFTEAGIIRYQSF
                     EGDLTLERDLPKQAFDLIVADLPCTGSGTWSRTPESLYFFDPDTVGRYGEIQRTILKN
                     IMHCMKPGGMLVYITCSVFAAENERVSAYLQDSGKLRLIREEMIGGFSERADSLFAAV
                     FRK"
     gene            complement(4566..>4941)
                     /locus_tag="VGM24_01435"
     CDS             complement(4566..>4941)
                     /locus_tag="VGM24_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY4154050.1"
                     /translation="SMAEIEKKVVELAGKARDNKLSMEEMQGGTFTITNGGVFGSLMS
                     TPIINIPQSAILGMHKIEERAVVVNGQIVVRSMMYLAVSYDHRIIDGRESVSFLVRVK
                     ELLENPDQLLFGKDPVKTLLEL"
BASE COUNT         1324 a         1242 c         1195 g         1180 t
ORIGIN      
        1 ctagtaaaag aagaatatgg aaaacatcct ggaccggggc ggagaggctt tttatttccc
       61 ggaatttttc ctggcggaag aaagcgaccg ttatttccag gagctgctcc gggaaattgc
      121 atggaaccag gaaccggtaa aaatattcgg gaagatgctg atgcagcccc gtttaacggc
      181 atggtatggg gatcccggca aaccctaccg gtattcgggc atcaccatgc atccgaaaaa
      241 ctggacgacc cctctgctgg aaataaaaca acggatagaa aaacggtcgc cggccctatt
      301 taacagcgcc ctgcttaatt tataccgcga tcaaaaagac catgtcggct ggcaccggga
      361 taatgaaaaa agcctggggg cacaaccggt catcggatcg gtcagcttcg gagcgacccg
      421 ccggttcctg ttccgctatt atgccgataa gaagattaaa cgcccgctgg agctgaccca
      481 tggaagtttt ctgctgatgc agggacaaac gcagcatttc tgggagcact gcattccaaa
      541 acaggccctg gccgcaggtc ccagaataaa tataacgttc agaaacattc aggagccggt
      601 atgaagatct cttaatccca ccatctggtg cttttccgcg tcccagatct ttaaccgaag
      661 acaggctatt acggcccggg gacttaaaga ggtccgtttg caattttgca gttccttgaa
      721 atcccgcatc acttccggca aacggtaaaa agggatttta gaattgaggt gatgaatgtg
      781 atgatacccg atattgccgg taagccaggc catcaccgga ttcatgacca tataactcga
      841 agattccatg gccgcttttt cataagccca ttcgttgttt tcggcaaacg tgacaccggg
      901 gaaattatgc tgggcataaa acaggtaagc gcccaaaccg aaagccacaa taaacggaag
      961 aaagaaaaga cagaaccaca tctgccagcc gaagaataca aaaataagaa tggatgctgt
     1021 tacgtgcagg accagagcga tcagggaatc atagtgtttc cgcgggttgc tcgcaaaaga
     1081 ggcaatgcac atgccgaaaa taaacatggt aaaataacca aggccgatgg tgagcggatg
     1141 gcggatggcc aggtaggcga atttatcgcg ggaagacatc tcctggaatt tctttctcga
     1201 aacgatcgga taggaaccga tgctggcgct gaataatttg gaattgtgat tgtggtggtg
     1261 atcgtgggaa cgcttccaga tactggtcgg cgaaagaata tagaatccaa aagccgtcat
     1321 gatgaaattt gcgatcttcg aatgggtgag tatggaatga tgctggtggt catgatagat
     1381 aatgaacatc ctgaccagca acaaaccgga aatgatactg aatactattc tgaccgcgag
     1441 gtaaggaatg aaaatgactc ccgcgaaggc aagcgccaga aaaaagatcg tagacaaagt
     1501 atggaaccag cttttaaccc ggacttcctt tgcataaggt ctggtcgcta aaataagttc
     1561 cttaccgtga agcatattcg aatgattaac tggtttttcg ggtatgtttc catctccggt
     1621 gtgtccacag ccagatctca ggctgccgcc tgatatcctc ttcgagtttc ctggtatgaa
     1681 actcggttat ctctcctggc cggctctccg ccaaaggtag ctcaattctt tcggcatgca
     1741 accggtaata gcctctttta actctttcaa tggagacata aaccaccggg tacctgatct
     1801 ttaacgctat tttctcaatg cctaaaaata cgggcgtatc ctggttcaga aagctcatcc
     1861 agtaagcccg gtccggttgc ggcgactggt ccgctatgaa agccgtcgcg ttcaattcat
     1921 tccggttgtt aagcatttcg cggagcgtat ccttcatgga aatcagtttg gtgccgaaac
     1981 gggtgcgcat cttataaacc agccggttga aatatttatt gctcagggga tgataaatta
     2041 catacaactg atgtttacaa agcaggctga aggtatttcc cgcccattcc cagtttccca
     2101 gatgccccat tacgatcata atgctttgac cggcagccgc cagctccttg aaaagattct
     2161 gcgactcctc ctccatccgg cagtgcttca gcatccgttc gcgggaaatg ctgagtgttt
     2221 taaacgtttc caggaaaaga tcgcagaaat accggtagaa ccggcgccgg atcaaaagga
     2281 gctccttttc cgttttttcc ggaaaggaat ttctcaggtt atttaatacc acttccctgc
     2341 ggtagccgag aacatggaac agcaggtagt agacgatatt ggataatccg tacaaaagcg
     2401 gaaagggaag aatggaaagc aggtaaatga aaggaagggc aaggtagtat aggatggcag
     2461 acaaaggtta acaattcgtt tgagccaaag ataaggctat ttattaccgg caaaccggta
     2521 ttaacatata aaaaccgcct tgtctggggc aataaatccg gtttgattcc gttatctttt
     2581 gttattattt tcccagatga aattgaaacc cggccttgtt ttcctgatct ctcttaccgc
     2641 attgatcttt tccggctgca ataaaaagaa ccctttaaaa acggatatcc cggctgacta
     2701 ttacccgatc caggtgggta aatatattat ctaccggatg gattcgctca agttcattaa
     2761 tatcggctcg caggatacca cgatcagcta tctcgccaaa gaagtggtgg aagacagcct
     2821 caccgataac ctgggccgcc cgagttaccg tgtggtccgt tacctgagcg acaccacggg
     2881 cacgcagcca tgggagcctt ccgtcgccta cctcgttacc ccaacttccc agtcggttga
     2941 agtggtggat aataaccttc gctttattaa actcgttaca cccgttcagg attatatttc
     3001 ctggaaaggc aatgtctata tcgataccaa atcggtgaat tcccaggttc cttatatgga
     3061 cgactggaac tacacttatg ccaacaccgg ccagccctat gatgtgctgg agggaacgat
     3121 accggaaacg gttattgtca atgaggagaa cgagacttcc ggaattgaag gctcgtacag
     3181 ccagacgatc atcagcgtgg aagtatatgg aaaagggatc ggtccgatct ataaaaattt
     3241 cctccattcg gaataccagg cgccgaattt gagcaacccc gccggaacaa ccatcggata
     3301 cgggctcacg tttaatatgg taagccataa ctgatccgca aaagccgatg ttctgcttct
     3361 cattatttcc tgaaaacagc agcaaataaa gaatcggcac gctccgaaaa tcccccgatc
     3421 atttcttccc ggatcaaccg caattttccg gaatcctgca ggtaagcgga cacccgctca
     3481 ttctccgcag caaaaacgga gcaggtgata taaacgagca ttccccccgg tttcatgcaa
     3541 tgcatgatat ttttgaggat cgtcctttgt atttcgccgt agcggccgac ggtatccgga
     3601 tcaaaaaaat acaaggattc cggcgtgcgg ctccaggttc cgctgcccgt gcagggcagg
     3661 tccgccacga tcaggtcgaa tgcttgtttc ggaagatccc tttccagcgt aaggtcccct
     3721 tcaaaggact ggtaccggat tatcccggct tccgtgaagc gcgtccgcag gttttccagg
     3781 atcgattttc ggatatctga caccgttaga tcgagcgtgg gataaagatc cgcgagcaga
     3841 atggatttcc ccccgctggc cgcgcagcag tcccaggccc ggataatttt ttccggatcc
     3901 gcatccggca ttttcagaaa gccggccgtt ttctgggaac tcaggtcctg caccaccgct
     3961 tcccggttca catccaggac accgtcgagg cgggtaccat tgggaaaaga acaggccgtg
     4021 aaaatcagcc ggtcatccgg cgccagctcc tggaaagcga tcttgttttc aaccagcttt
     4081 ttccgcacca cggcttcctt ccctttccgg atacgtataa acaattccgg cttttttaaa
     4141 aaagacaaac aataggctgc aggatccact cccgcagaga gcaggtcttt aaaaggaaat
     4201 atctggtcaa tggaaaaccc cgggaatttt tttttcaata tctgaacctt ctcttccacc
     4261 ggcatctcta tcgcggcgtt ccaatccggc gcaaggaatt ccaatgcctg catcccgcgc
     4321 tggttgcaca ggaacattcc ggcgaggata cgttcgccgg atcctgcctc cggcagggaa
     4381 cgacccagac ggtaaaaaca gtaaaccatt tcgctcacca gcttccggtc gcgcgaaccc
     4441 atttgcggat tggcccggaa gaattcttta agccatacat gcaggggttt ttcccccgaa
     4501 taggcatcaa cgatccggac ggcataccgg agcaggttgt caaacatcat gcggggattg
     4561 aagcatcaaa gctcaaggag cgtctttacc ggatcctttc caaataacaa ttgatcggga
     4621 ttttccagca attctttaac gcggaccagg aagctgacgg attccctgcc gtcgatgatg
     4681 cggtgatcgt aactgaccgc cagatacatc atggaccgga ccacgatctg gccgtttacg
     4741 accacggccc tttcttctat tttatgcatg ccgaggatgg cagactgggg aatattaatg
     4801 atgggagtag acatcaggga gccgaacaca ccgccattgg taatggtaaa cgtgccgccc
     4861 tgcatttctt ccatgctgag tttattgtcg cgcgctttgc ctgcgagttc taccaccttt
     4921 ttctcaatct ccgccatgct c
//
LOCUS       DASYCU010000074         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4514769, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000074 DASYCU010000000
VERSION     DASYCU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4514769"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /gene="serS"
                     /locus_tag="VGM24_01440"
     CDS             complement(<1..1166)
                     /gene="serS"
                     /locus_tag="VGM24_01440"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY4154051.1"
                     /translation="MLQLAVIRQQPDWIEERLRVKNFEAGEAIRSILSLDEQRKLLQL
                     ELETAQAQLNSISKEIGQWIARGDKAKAEEKKQAVSAFKTAIPPLQEKLNRLDQQLHE
                     LLVILPNLPAKEVPAGKTPEDNQIVREGGERPGLPEGAVPHWDLAKKYNLIDFELGNK
                     ITGSGFPVYINQGARLQRAMIQYFLDFNTGAGFTEYQPPLMVNEASAFGTGQLPDKEG
                     QMYFAAEDKFYLIPTSEVPLTNIFRDVIVKENELPIRMTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIVHPEKSYAALEEMVLHVERLLQSLKLPYRILRLCGGDMSFASA
                     ITYDFEVFSAAQEKWLEVSSVSNFESFQTNRMKIRFKDAQGKMNLLHSLNGSSLA"
     gene            1234..1581
                     /locus_tag="VGM24_01445"
     CDS             1234..1581
                     /locus_tag="VGM24_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154052.1"
                     /translation="MDGKEDLQVRWWALEAKLMERFGKKPDMEALLFLVGIQELGEIR
                     KKFTKEQKQDLMHVAVCSLFAQSGYYELEKVDEEGWPHFRQLKALPVMNMMEQENFMK
                     DHMLLYFEKQGLD"
     gene            1664..2044
                     /gene="gcvH"
                     /locus_tag="VGM24_01450"
     CDS             1664..2044
                     /gene="gcvH"
                     /locus_tag="VGM24_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY4154053.1"
                     /translation="MSFPKNLRYTKEHEWVRLEDGQTAIVGITEYAQSELGDIVYVEV
                     ETIGRTLEAGAVFGTVEAVKTVSDLFLPVAGTIAELNPELASAPDLVNSDPYGKGWMI
                     KLKLTDSSSVNDLLDAEAYAALVG"
     gene            2082..2621
                     /locus_tag="VGM24_01455"
     CDS             2082..2621
                     /locus_tag="VGM24_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4154054.1"
                     /translation="MGSTSTYNEQDLIMALKARNNQAFGYLYDHYGGSLYSIILQIVQ
                     DPTLAKDVMQEVFISIWRRIDTYDPAKGKLFTWMLNISRNASIDLLRSRNYLNSRKNQ
                     PVDDIVYKNIRATQTGTDSIGLTKFLERLRPDHRVLLELAYFKGYTQEEIAQIQEIPL
                     GTVKTRIRNALLQLREYLK"
     gene            2622..>2923
                     /locus_tag="VGM24_01460"
     CDS             2622..>2923
                     /locus_tag="VGM24_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154055.1"
                     /translation="MNIQEYISSGIVESYVLGLASPEEGREFERICSEQAEVLAAREA
                     FEKELEYHLQQSAIPPPRELKSSIFSRIGMEEEPQTPQNDRNKRVVPGKPERVPRLG"
BASE COUNT          775 a          700 c          726 g          722 t
ORIGIN      
        1 gcgagcgaac tgccgttaag cgagtgcaac aggttcattt taccctgtgc atccttaaac
       61 cggatcttca tccggttggt ctggaagctt tcaaaattgc ttaccgaact cacttccagc
      121 catttttcct gggcggcgct gaatacttca aaatcatagg taatagcgga cgcaaaagac
      181 atatcgccgc cgcatagccg gagaatgcga taagggagct ttaacgattg aagcaatctt
      241 tctacgtgaa gcaccatttc ttccaaagcc gcataacttt tttcaggatg gacgatctgt
      301 acgatctcca ccttatcgaa ctgatgcaac cggttcaaac cccgcacgtc tttcccgtag
      361 cttcctgctt cccggcgaaa acagggcgta taggcagtca tccggatggg aagttcattt
      421 tcttttacaa tcacatcccg gaagatattg gtcagcggca cttcggaggt gggaatgaga
      481 taaaatttat cttccgcggc gaaatacatt tgtccttcct tatccggcag ttgtccggtg
      541 ccaaaggcac ttgcttcatt caccattaac ggaggctgat attcggtaaa tccggctccc
      601 gtattgaaat ccagaaaata ctggatcatg gcccgctgca aacgggcgcc ctggttaatg
      661 tataccggga atccgctgcc cgtaatttta ttcccgagtt caaaatcgat caggttatat
      721 ttttttgcca ggtcccagtg tggcaccgcc ccttcgggaa gccctggacg ttcgccgccc
      781 tcccggacga tctgattgtc ttccggtgtt tttcctgcgg gaacctcttt cgccggaagg
      841 ttagggagaa tgaccagaag ttcatgcagt tgctgatcca gccggttcag tttttcctgt
      901 aaaggcggaa tggccgtttt aaaagccgac acggcctgtt ttttttcctc ggcctttgct
      961 ttgtcgcccc gggctatcca ctgaccaatt tcctttgaaa tggaatttaa ttgtgcctgg
     1021 gctgtttcaa gttccagctg gagcagtttg cgttgttcat ccagggaaag aattgaacgt
     1081 atggcctccc cggcctcaaa attttttacc ctcaaccgct cttctatcca gtctggctgc
     1141 tggcggataa ccgctaactg taacatgaac ctaaaatttt ggcaaaggta accgaactgc
     1201 acatagattc ctgcaccggg ttatctttgc cggatggatg gaaaagaaga tcttcaggtc
     1261 cgctggtggg cgctggaggc gaaactgatg gaacgttttg ggaaaaaacc ggatatggaa
     1321 gccctgcttt ttctggtcgg catccaggag ttaggtgaaa tcaggaaaaa gtttaccaaa
     1381 gaacaaaagc aggacctgat gcatgtagct gtctgcagcc tgtttgccca gagcggatat
     1441 tatgaactgg aaaaagtgga tgaagaagga tggccgcatt tccgccagct aaaagccctg
     1501 cccgtaatga atatgatgga gcaggaaaat ttcatgaaag atcacatgct cctctatttt
     1561 gaaaagcagg gactggactg aacgaagatt tcccccggcg ggaagaatat ccaaatcctt
     1621 ctatatttgc actggccttt ttcaacacga ataaatttat tacatgagtt tcccaaaaaa
     1681 tctccgctac acgaaagagc acgaatgggt ccggctggaa gatggacaaa ccgctattgt
     1741 cggaattacg gaatatgcgc aaagcgaatt gggcgacatt gtttatgtgg aagtggaaac
     1801 catcggacga acccttgaag cgggagctgt ttttgggacg gtggaagcgg taaaaaccgt
     1861 atccgatctt tttcttccgg ttgccgggac catagcggag ctgaatccgg aattggcctc
     1921 cgcacccgac ctcgtaaaca gcgatcctta tggcaaagga tggatgatca aactaaagct
     1981 gactgattcc tccagcgtta atgatctgct ggatgcggaa gcctacgctg ccctggtggg
     2041 ttgattcaga ctgggtatga tacttctatt taccggaata cttgggttca acttcaacat
     2101 acaatgagca ggatctgatc atggccctga aagccaggaa taatcaggcg tttggctacc
     2161 tctatgacca ttacggcggg tccctttaca gtataatatt gcagatagtt caggatccaa
     2221 ccctggccaa agatgtgatg caggaggttt tcataagtat ctggcgcagg atcgatacct
     2281 atgatccggc taaaggaaaa ctatttacct ggatgctgaa tatcagccgg aatgcctcca
     2341 tagacctgct caggtcaaga aattacctga acagccggaa aaaccagccc gtggatgata
     2401 tcgtatataa aaatatccgg gcgacccaaa ccggtacaga cagcattggt ttaacaaaat
     2461 tcctggagag actccggccg gaccaccgcg ttttgctgga acttgcctat tttaagggct
     2521 atacgcagga agagatcgcg caaattcagg aaatacccct gggaaccgtc aaaacacgaa
     2581 tccggaatgc tttattgcag ttaagagaat atttgaaata agtgaacatt caggaataca
     2641 tatcgagcgg aatagttgag agctatgttc tcgggttagc cagccccgag gaaggaaggg
     2701 aattcgagcg catctgttcc gagcaggcgg aggttcttgc tgcccgggag gctttcgaaa
     2761 aagaactgga ataccacctg caacagtcgg cgatcccccc tccgcgcgaa ttaaaatcca
     2821 gcatattttc ccggatcggt atggaagaag aaccgcaaac cccgcagaat gaccggaata
     2881 aacgggtagt cccgggcaaa ccggaacggg ttccccggct agg
//
LOCUS       DASYCU010000075         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4514823, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000075 DASYCU010000000
VERSION     DASYCU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4514823"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VGM24_01465"
     CDS             complement(<1..573)
                     /locus_tag="VGM24_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY4154056.1"
                     /translation="MMKYWIFFLSLVLSLETGQAQPGPDGQQNQIRPPAYSKFVPGQR
                     WYDSNGELINAHGGGILYIHKKYYWFGEKRGKRGSGGINLYSSNDLYNWKYEGLVLAP
                     DEADTTSDISKGCSMERPKVIYNKKTGRYVLWFHLELRGKGYSAARAGVAVSDKIAGP
                     YKFIGSSRPNGNMSRDMTLFKDDDGSAYIIY"
     gene            755..1855
                     /locus_tag="VGM24_01470"
     CDS             755..1855
                     /locus_tag="VGM24_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154057.1"
                     /translation="MYFSFDKITGDGSNKPSRKKPIFPVNDLLRDYLKQHGREVRLPV
                     SYNDLRLIAYAVPLKDKEGIDALWETVSYDMQHWQYIRAGLVKMYAILKTEGDMTFSK
                     HLDVARVDFCSFANSSPFRIRIVNKFNDNYDHYYVKQADASRVYGLELEHLLSPNRIT
                     YFTHNSTLIEEHIPGIPGDVFIRKYLDEPITNRIRFAKEFVKFNERCFVRLLGDMRSY
                     NFVVNIIPDIEDFQYRIRSIDFDQQSYEGRKSLYLPQFFKENKALVDLCIKYLNRDSI
                     TQYQTEERTMMAFRVASSRFRLMHLLNIMSKDQISTPEKLMQLKQELAQAFDAPIFLK
                     SRSMGQVVKRQLKQTLQKNLALIQKNLGRFED"
     gene            1965..3032
                     /gene="fbaA"
                     /locus_tag="VGM24_01475"
     CDS             1965..3032
                     /gene="fbaA"
                     /locus_tag="VGM24_01475"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY4154058.1"
                     /translation="MAKYKAGVLYGEELEALYQDAKDSQFALPAVNVTGTDTINAALE
                     AAAKVNSPIIIQFSNGGAQFVAGKGMPNDKLQANISGGVAGALHIHNVAKYYGVPVIV
                     HTDHAAKKWLPWISGLLDAGEQYFREKKQPLYSSHMLDLSEESLEENIHTSVEFFKRM
                     KPLGMAIEIELGVTGGEEDGVDNSGVENDKLYTQPQHVAYAYTELSKVGRLFSVAAAF
                     GNVHGVYSPGNVELRPVILKNSQDFIEKELKTKPKPVYFVFHGGSGSPQNQIREAIGY
                     GAIKMNLDTDLQWAFWEGVQKYYKKNEAYLQAQLGNPEGADRPNKKYYDPRVWLRKGE
                     ESFISRLQTAFEDLNCVNRNA"
     gene            3132..>3335
                     /locus_tag="VGM24_01480"
     CDS             3132..>3335
                     /locus_tag="VGM24_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5118 domain-containing protein"
                     /protein_id="HEY4154059.1"
                     /translation="MKFFLTVLFCAGLSVPVLSQRKPRTPLPKPATDSTHPVKPKNPI
                     AGNPKPYKEVIPDSAVTQKGLFTV"
BASE COUNT          898 a          842 c          776 g          819 t
ORIGIN      
        1 ataaatgata tacgctgaac catcgtcatc tttaaaaaga gtcatgtccc tcgacatatt
       61 tccattcgga cgggaacttc cgatgaattt ataggggcct gctattttat cgctaactgc
      121 aacccctgcc cgggcggcgc tatagccctt ccctctcaat tccaggtgaa accacaggac
      181 gtacctgccc gtcttcttgt tataaataac tttcggccgc tccatactac agcctttgct
      241 gatatcactt gtcgtatcgg cctcatcggg agccaatacc aacccttcat atttccagtt
      301 gtataaatca tttgaagaat acaggtttat tcccccggaa ccccttttgc cccttttctc
      361 tccaaaccag taatactttt tatgaatgta aagaataccg cccccgtgcg cattgatcag
      421 ttctccgttg ctgtcgtacc agcgttggcc gggaacaaat ttactatagg ccggaggccg
      481 gatctggttt tgctgcccgt ccggaccggg ttgtgcttgt cctgtctcta aactcaaaac
      541 caggctcagg aaaaagatcc agtatttcat cattacatcc cttttgtttt ccgcccttta
      601 atggccggca tattaaaaaa acgaacgccc ggaaattgat caccggaatt cttcgggaga
      661 agcaaaattt gaattcaccg gattcagaga tgcccgggac gacccggatc ctaccctgct
      721 gatctccatt atagcgttcc gcgtatattt gcctatgtac ttttctttcg acaaaattac
      781 gggagatggt tccaataaac cttcccgtaa aaaacccatt ttcccggtca atgatttatt
      841 gcgggactac ctgaaacagc acggaaggga agtaagactg ccggtttcct acaatgatct
      901 gcgtctgatc gcatatgccg ttccgctgaa agacaaggaa ggcatagatg ctttatggga
      961 aacggtctcc tatgatatgc agcactggca gtatatccgg gccgggctgg tgaaaatgta
     1021 cgccattctg aaaacagagg gagacatgac cttcagcaaa cacctggatg tggccagggt
     1081 ggatttctgc agttttgcga attccagccc ttttcgtatc cggattgtaa ataaattcaa
     1141 cgacaattac gaccattact atgtgaagca ggccgacgca tcgcgggttt acgggctgga
     1201 gctggaacac ctgctttctc ccaaccgcat tacgtatttc acgcataaca gcacgttaat
     1261 tgaagaacat atccccggga taccgggaga cgtctttatc cgtaaatacc tggatgaacc
     1321 catcaccaac cgcatccgtt ttgccaaaga atttgtgaag ttcaatgaac ggtgttttgt
     1381 ccgcctcctg ggagatatgc gctcgtataa ttttgtggtg aatatcatac cggacatcga
     1441 ggatttccag taccgcatcc gttccattga ttttgatcag caatcctatg aaggccggaa
     1501 aagcctctac ctgccccaat tctttaagga aaacaaagcc ctggtggatc tgtgcataaa
     1561 atacctgaat agggattcga taacccagta ccagaccgag gaaagaacca tgatggcttt
     1621 tcgcgtggcc tcctcacgtt tccggctgat gcatttgctg aatattatgt cgaaagacca
     1681 gatctcaacg ccggaaaaat taatgcagct aaaacaggag ctggcacagg catttgatgc
     1741 accgatcttt cttaaatccc ggtcgatggg ccaggtggtg aaacgccagc tcaagcaaac
     1801 ccttcagaaa aatcttgcgc tcattcaaaa aaacctgggc cggttcgaag attgagcgat
     1861 atcccggata tgagacgccg gaattaaccg gttttttacc cgtcttgcat cgtcaaaacc
     1921 ctggggaacc cttatctttg cggcttctta tttaaaaaat aaatatggcc aaatacaaag
     1981 caggcgtttt atacggcgaa gaactggaag ccctttacca ggatgcaaaa gacagtcagt
     2041 ttgcgttacc ggcggtaaat gttacgggca ccgacaccat caatgcggcg ctggaagcag
     2101 cggcaaaagt gaattctccc atcatcattc aattctccaa cgggggtgcg caattcgttg
     2161 ccggaaaagg aatgcccaat gataaactgc aagccaatat ttccggtggg gtagccggag
     2221 cccttcatat tcataatgtg gctaaatatt atggcgttcc ggtgatcgtg cataccgatc
     2281 atgcggcaaa gaaatggctt ccctggatca gcggcctgct ggatgccggt gaacaatatt
     2341 ttagagagaa aaagcaaccg ctatatagtt ctcacatgct ggatctgtcc gaagaatcac
     2401 tggaagaaaa catccatact tccgtggaat ttttcaaacg gatgaagccg ctgggcatgg
     2461 ccattgaaat cgagctcggc gtgaccggcg gggaagaaga cggcgtggac aacagcgggg
     2521 tggaaaatga taaactctat acccaaccgc agcacgtggc ctatgcttat accgaactca
     2581 gtaaggtggg ccgtttattc agtgtggccg ctgccttcgg aaacgtgcac ggggtgtaca
     2641 gcccgggcaa tgtggagctt cgtcccgtga tcctgaagaa cagccaggat tttattgaga
     2701 aggaattaaa aacaaaaccc aagccggtct attttgtatt ccatggcggc agcggatccc
     2761 cgcagaacca aatccgtgaa gccattggct acggcgccat caagatgaat ctcgacaccg
     2821 acctgcagtg ggcgttttgg gaaggggtac agaaatacta caagaaaaac gaagcctacc
     2881 tccaggccca gctgggcaat ccggagggag ctgaccggcc gaataaaaaa tattacgatc
     2941 cgcgggtctg gttacgcaaa ggcgaagaaa gttttatcag ccgccttcag acggcatttg
     3001 aagatttgaa ttgtgtgaac agaaatgcct gattcattcc gttaccggat cctaccgtcc
     3061 atcaactatc cgtgcccgta agcgggcatt ctgcgtaaaa tccatatatt cattctccat
     3121 taagaccatt tatgaagttc ttcctgaccg tcctgttttg cgcgggcttg tcggtcccgg
     3181 ttctttcgca aagaaaaccc cggacgccac tcccgaagcc ggctacagac agcacccatc
     3241 cggttaaacc caaaaatccc atagccggca atcccaaacc ctataaggag gtgataccgg
     3301 acagtgcggt tacgcagaag ggtttgttca cggtt
//
LOCUS       DASYCU010000076         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1616408, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000076 DASYCU010000000
VERSION     DASYCU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616408"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VGM24_01485"
     CDS             complement(<1..711)
                     /locus_tag="VGM24_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154060.1"
                     /translation="MKKLIAVFLMVSAAWAVQAQSSREGMLYLNKPLSGDAIQQVEAN
                     TSGGGIQVSGVQPSEARIEVYISGNEGRFSGLSKEEIQKRLDADYDFSVTVSNHKLTA
                     NARTKKGFRDWRKSLNISFSIYVPNEVSTELATSGGGISLQNLSGTQDFRTSGGGLHV
                     KEVSGHITGRTSGGGIHVSDSKDDIDLETSGGGIEAVHCSGKIHLNTSGGSLHLTDLD
                     GTITATTSGGGVDADRIKG"
     gene            complement(758..1258)
                     /locus_tag="VGM24_01490"
     CDS             complement(758..1258)
                     /locus_tag="VGM24_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154061.1"
                     /translation="MLKNYLLIAIRNLKKDRIFSFINILGLACSLLILLWIQDERSMD
                     KFNKNTSRLFSVYERQYYDNKVDAFHSTPGVLADEMKRVLPQVQYASGMAWENPSTFQ
                     VGEKIMQETGNHAGMVDVCAGGDCSHSDYFIYGELPGAESGSCQSGEKPAYGVGPGTP
                     ETFIMT"
     gene            complement(1538..1690)
                     /locus_tag="VGM24_01495"
     CDS             complement(1538..1690)
                     /locus_tag="VGM24_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154062.1"
                     /translation="MGNGKQIEKGDFNGWSNSRVVIAVKLVQKYDNPEHPAMEAIQWF
                     VQLFHF"
     gene            1744..2304
                     /locus_tag="VGM24_01500"
     CDS             1744..2304
                     /locus_tag="VGM24_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY4154063.1"
                     /translation="MTDNHKLIRDCIRGKPAAQRELYEQFAGNMLGVCYRYTKSMSDA
                     EDVLQEGFVKVFRYLEQFKGDGELAGWIRRIMVNTAINYLKRNRHYQADLSFTDPGLH
                     PVSTDNPDVQINAKELADLIRQLPPGYQTIFNLYAVEGYSHIEIGKMLGISNVTSRSQ
                     YSRARALLITWLEKESDDLKKNSYAR"
BASE COUNT          635 a          608 c          569 g          649 t
ORIGIN      
        1 tcctttgatc cggtcagcgt ccactccgcc tccgctggtg gtagcagtga tcgttccgtc
       61 caggtcggtc aaatgcagcg acccgccgga agtattcagg tggattttgc cgctgcaatg
      121 aacggcctcg atcccgccgc cactggtttc cagatcgata tcgtctttag aatcagaaac
      181 atggattccc ccgccggacg tacgcccggt aatatgcccg gaaacctctt tcacatgcag
      241 gccgccgccg ctggttctga aatcctgcgt tccggaaagg ttttgcaggc ttattcctcc
      301 cccactggtg gccagttcgg tggaaacctc gttgggaaca tagatggaaa atgaaatgtt
      361 caggcttttc cgccaatcgc gaaatccctt ttttgtcctg gcattggctg tcagtttatg
      421 attggagacg gtcactgaaa aatcatagtc cgcatccagc cgcttttgaa tttcttcttt
      481 ggacaatccc gaaaagcggc cttcgtttcc ggatatatac acttcaatcc gtgcttccga
      541 tggctgtacg ccggatacct gtataccgcc tccggaagta tttgcttcca cctgctggat
      601 cgcatccccc gataagggtt tgttgagata tagcattccc tctctggaag attgggcctg
      661 gacggcccat gcggcgctga ccatcaggaa aacggcaatc aattttttca taaactgttt
      721 tttttattaa tgttaggatt tctatttaaa aagttattca ggtcattata aaagtttcag
      781 gggtacccgg ccctactccg tacgcaggct tttcaccgga ttggcaagag ccgctttcag
      841 cgcctggtaa ctcaccgtaa ataaagtaat cagaatggct acaatccccg ccagcacaaa
      901 catcaaccat cccggcatgg ttgcccgttt cctgcatgat tttttctccc acctggaagg
      961 tgctcggatt ttcccaggcc atgccactgg catattgcac ctgcggcagc acccgtttca
     1021 tttcatctgc cagcactccc ggagtggagt gaaaggcatc tactttatta tcgtagtact
     1081 gccgttcgta aacgctaaat aaccggcttg tgtttttatt aaatttatcc atgctgcgtt
     1141 catcctgaat ccacaacagg atcaggagac tgcaggcgag gcccaggata ttgatgaaag
     1201 aaaagatcct gtcttttttc agatttctga tggcgatcag cagatagttt tttagcatca
     1261 gtgaaaattt tagggattgt gcgcttggcc cgctgaataa ttcagtctaa ccaactcaat
     1321 gccagatctg taaatctcta cataccaata atttattctg tgatcaatgg aagaaactgc
     1381 ccggttttga tacgctgcct gtccgcttta accagataaa aagccggccc ggggcctcga
     1441 tttgggagaa agacccgtta atccggaatt tgttgcacgg ggagggaaaa caaaatgaag
     1501 aataaggaga aaaaccctgc atttttaaac caattagtta aaaatgaaat agttgcacaa
     1561 accattggat tgcttccatg gcgggatgct ccgggttatc gtatttttga accagcttta
     1621 ccgcaatgac cacccttgaa tttgaccagc cattgaaatc gcccttctct atctgtttcc
     1681 cgttacccat tttatatctt tcatgcaacg gtttatcccg aatacttgtc aacctcatac
     1741 cagatgaccg ataatcataa actgatcagg gactgcatcc ggggaaagcc ggcagcccag
     1801 cgggagttgt atgagcaatt tgcaggcaat atgctgggcg tatgttaccg gtacaccaaa
     1861 agcatgtccg atgccgaaga tgttttgcag gagggctttg taaaggtatt ccgctatctg
     1921 gaacagttta agggggacgg cgagctggca ggatggatcc gcagaattat ggtgaatacc
     1981 gcaataaatt atctgaaaag aaaccggcat tatcaggctg atctctcctt tacagatccc
     2041 gggctccatc ccgtttcaac cgacaatccc gacgtgcaga taaatgcaaa ggaactagca
     2101 gacctgatcc ggcaattgcc accgggatat cagacgattt ttaatctgta tgccgttgaa
     2161 gggtattcac atattgaaat cggaaagatg ctgggcatca gcaatgtcac ctcccgctcg
     2221 cagtattccc gggcaagagc cctattaatc acctggctgg aaaaagaatc ggatgattta
     2281 aaaaaaaata gttatgccag atagtcattt tgaaaatagc gccaaacaaa agctggatga
     2341 actgacgttc agcccatccg gtgctgtctg gcaaaaagtg gaggctcaga taaaaaagga
     2401 taaagaccgc cggaggctga tgatctggct ccccctgtta tgtctcctgc tgggagccgg
     2461 c
//
LOCUS       DASYCU010000077         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3934598, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000077 DASYCU010000000
VERSION     DASYCU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3934598"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VGM24_01505"
     CDS             <1..440
                     /locus_tag="VGM24_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY4154064.1"
                     /translation="KYFGGGLKGKHFALWGLAFKPNTDDIREAPALYIIDALIKEGAS
                     LAVYDPEAMKNVKAQIGDVVVYGESQYDVLNSADALIIATEWNEFRTPNFLKMVTSMK
                     NKVIFDGRNLFDINAIRELGFYYESVGRATAIPVNNNYHKKDA"
     gene            437..1417
                     /locus_tag="VGM24_01510"
     CDS             437..1417
                     /locus_tag="VGM24_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY4154065.1"
                     /translation="MSKKRVLITGAAGFLGSHLCDRFIREDFLVTGMDNLITGDLKNI
                     QHLFKLKEFEFYHQDVSKYIHVPGQLDYILHFASPASPIDYLKIPIQTLKVSSLGTHH
                     CLGLAKAKNARILVASTSEIYGDPQVHPQPEEYWGNVNPVGPRGVYDEAKRFQEAITM
                     AYHTFHKLETRIVRIFNTYGPRMRLNDGRALPAFIGQALRGEDLTVFGDGSQTRSFCY
                     VDDLIEGIFRLLMSDYALPVNIGNPSEITLQDFAEEVISLTGTNRKIIYKPLPADDPR
                     QRKPDISKAKKILDWEPKVDRREGLKITYDYFKSLPPEEWYKLPKEFESK"
     gene            1472..2533
                     /gene="rfbB"
                     /locus_tag="VGM24_01515"
     CDS             1472..2533
                     /gene="rfbB"
                     /locus_tag="VGM24_01515"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY4154066.1"
                     /translation="MTEYKKTIMITGGAGFIGSHVVRLFVNKYPEYRIVNLDALTYAG
                     NLENLRDLQGAPNYIFEKGDITVTGEITGLFEKYHFDSVIHLAAESHVDRSIQDPLAF
                     VKTNVMGTCTLLNICRQQWKANTERKLFYHVSTDEVYGSLGPTGFFTEASPYDPRSPY
                     SASKASSDHFVMAYHHTYGLPVILSNCSNNYGSHHFPEKLIPLMINNIKEMRPLPVYG
                     KGENVRDWLYVDDHARAIDTIFHKGRVGEKYNIGGFNEWKNIDIVHLLCRIMDKKLGR
                     APGESAKLISFVKDRPGHDLRYAIDAGKLNQELGWKPSLQFEEGLEKTVDWYLQNEEW
                     VKKVTSGEYQHYYEKQYQK"
     gene            2542..3102
                     /gene="rfbC"
                     /locus_tag="VGM24_01520"
     CDS             2542..3102
                     /gene="rfbC"
                     /locus_tag="VGM24_01520"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY4154067.1"
                     /translation="MPFTETPLPGLFVFEPSVYPDERGYFFESYNKRVFGENAIRNEF
                     VQDNQSFSCYGVIRGLHFQREPHAQSKLIRVLRGRVLDVVVDLRAGSPGFGKSFSIEL
                     SSENKKQLFIPRGFAHGFSVLSETTEIAYKCDQFYNKQSEAGIRYNDPTLKIDWKIPS
                     DKSLISEKDALLPEFDPGITIFKFSE"
     gene            3104..3985
                     /gene="rfbD"
                     /locus_tag="VGM24_01525"
     CDS             3104..3985
                     /gene="rfbD"
                     /locus_tag="VGM24_01525"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY4154068.1"
                     /translation="MTEKKISTIAVSGMNGQLGKELAVLAPHFPQFEFLFLSREVFPL
                     DVPEKIESWLTGHPVDVLINCAAYTAVDKAESESGMANQINGEAPGIISGLLSRSGGK
                     LIQLSTDYVFDGLSSRPLDEEAVTDPVNIYGASKLMGERAAMANNPQTLILRTSWLYS
                     AFGNNFVKTMIRLLKTKESIRVVSDQKGSPTYAADLASAILLILSAEKFIPGIYHYSN
                     EGETTWFDFASEIKEMTGSSCTVSPVESSGYPTAARRPAYSLLDKSKIKKNYGLTIPG
                     WKTSLAACLEILLRSEV"
     gene            complement(4007..4255)
                     /locus_tag="VGM24_01530"
     CDS             complement(4007..4255)
                     /locus_tag="VGM24_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154069.1"
                     /translation="MEAIIDLLQAIQLNMLEVTIAGLFIFGIGYLLGTKKVRRLTQEV
                     YGLQKDVLDLNEELLYGVSDTPVIGLKPENLKQAKFAK"
BASE COUNT         1222 a         1074 c         1055 g         1069 t
ORIGIN      
        1 aaaaatattt tggcggcggt ctgaaaggca agcattttgc gctctggggc ctcgccttca
       61 aacccaatac cgacgatatc cgggaggcgc ctgccctgta tatcatagac gccctgataa
      121 aagaaggcgc cagcctggct gtatatgatc cggaagcaat gaaaaacgtc aaggcccaga
      181 tcggggatgt ggtggtttac ggagaaagcc agtacgatgt gctcaacagc gccgatgcgc
      241 tgatcattgc cacggagtgg aatgaattcc ggacgccgaa ttttctgaaa atggttacca
      301 gcatgaagaa caaagtgatc ttcgacggaa gaaacctgtt tgacatcaat gcgatccgcg
      361 aactgggatt ctattatgaa agcgttggcc gggccacggc cattccggtg aacaataatt
      421 atcataaaaa ggacgcatga gcaaaaaacg tgtactcatt acgggtgcag ccgggttttt
      481 aggctctcat ctctgcgacc ggttcatccg ggaggatttc ctggttaccg gaatggacaa
      541 cctgatcacc ggcgatttaa aaaatatcca gcacctgttc aaactgaaag agtttgaatt
      601 ctatcaccag gatgtcagca aatacattca tgttcccgga caactggatt atatcctgca
      661 ttttgcgtcc ccggcgagcc cgatcgatta tttaaagatc ccgatacaga ccttaaaagt
      721 gagttccctc ggcacccacc attgcctcgg actggcaaag gcaaaaaacg cccgtatcct
      781 ggttgcttcc acttctgaaa tctacgggga tccgcaggtc cacccccaac ccgaagaata
      841 ctggggaaat gtgaatcccg tcggcccccg cggcgtctac gatgaagcca aacgttttca
      901 ggaagccatc accatggcct accatacttt tcataaactc gaaacccgca tcgtgcggat
      961 cttcaatacc tatggcccgc gcatgcggct caacgacggg cgtgcgctgc ctgcatttat
     1021 cggacaggcc cttcggggag aagacctgac ggtattcgga gacggcagcc agacccgttc
     1081 tttctgttat gttgacgacc tcattgaagg aatcttccgt ttgctgatga gcgattatgc
     1141 ccttccggta aacatcggaa atccctccga gatcacgctg caggattttg cggaggaagt
     1201 gatctccctc accggaacaa accggaagat catttacaag ccgcttccgg cagatgatcc
     1261 aagacagcgc aagcccgata tttccaaagc aaaaaaaata ttggactggg aaccgaaggt
     1321 tgaccgccgg gagggtctga agatcaccta cgactatttt aaatccctcc cgccggaaga
     1381 atggtacaaa ctgcctaagg aatttgagag caaatgatcc cggataaaaa tacgggaaca
     1441 aatgggttaa cctgaaatac tgaaccttac catgacagaa tataaaaaaa cgatcatgat
     1501 caccgggggc gcgggcttta tcggctccca cgtggtcagg ttatttgtga acaaataccc
     1561 ggaataccgg atcgtcaacc tcgacgcact aacctatgcc ggcaacctgg aaaacctccg
     1621 cgacctgcag ggcgccccca actatatttt tgaaaaggga gacattaccg ttaccggaga
     1681 aatcacggga ttatttgaaa aataccattt tgattccgtg atccacctgg cagcagaaag
     1741 tcatgttgat cgctccatcc aggatccgct ggcctttgtg aagaccaatg tgatgggcac
     1801 ctgcacgctg ctgaatatat gccggcagca atggaaggcg aatacggaaa gaaaattatt
     1861 ttaccatgtt tccacggacg aagtgtacgg ttccctggga cccaccgggt tttttacgga
     1921 ggccagtcct tacgatcccc gatctcccta ttcggcatca aaagcttctt ccgatcattt
     1981 tgtgatggct taccatcata cgtacgggct tcccgtcatc ttgtccaact gttccaataa
     2041 ttacggttcg caccattttc cggagaaact catcccgctg atgatcaata atattaagga
     2101 gatgcggccg cttccggtat atggaaaagg ggagaatgtg cgcgactggt tatacgtgga
     2161 cgatcatgcg cgggccatcg ataccatatt ccataaggga agggtgggcg aaaaatataa
     2221 tatcggcgga ttcaatgaat ggaaaaatat tgatatcgtg catttacttt gccggatcat
     2281 ggataaaaag ctgggccggg cgccggggga atccgccaaa ctgatcagtt ttgtaaagga
     2341 ccggccgggc catgacctgc gctatgccat tgacgccggt aaactgaatc aggaactggg
     2401 gtggaagccc tccctgcagt ttgaagaagg gttggagaaa acagtggact ggtatctgca
     2461 gaatgaggaa tgggttaaaa aggtaacttc cggcgaatat cagcactact acgagaaaca
     2521 atatcagaaa taattccagt catgcctttt accgaaacgc cgttacccgg actttttgtt
     2581 tttgaacctt cggtctaccc cgacgaacgc ggctattttt tcgaatccta caacaagcgc
     2641 gtatttgggg aaaacgcgat ccggaatgaa ttcgtgcagg ataaccagtc tttctcctgt
     2701 tacggcgtta tccggggatt gcatttccag cgtgaacccc atgcccagtc caagctcatt
     2761 cgtgttttac ggggcagggt tttagatgtc gtagtggacc ttcgggcagg atctcctgga
     2821 tttggaaaaa gtttcagcat cgaactttct tcagaaaata aaaagcagct ttttattccc
     2881 aggggttttg cccatgggtt ttctgtttta agcgaaacaa cggaaatcgc ttataaatgc
     2941 gatcagtttt ataacaaaca aagcgaagcc ggtatccggt acaatgaccc gacgttaaaa
     3001 atcgactgga agattccttc cgataaatcg ctgatctcag aaaaggatgc attgctacca
     3061 gaatttgatc cgggtattac gattttcaaa ttcagcgaat aagatgacgg aaaaaaaaat
     3121 aagcaccatt gcggtcagcg gcatgaatgg ccagctggga aaagaactgg ccgtgctggc
     3181 gccacatttt ccgcaatttg aatttttgtt tctttccaga gaggtattcc cgctggacgt
     3241 cccggagaaa atcgaatcct ggctgaccgg tcatcccgtt gatgtattga ttaactgcgc
     3301 cgcctataca gcggttgaca aagccgaatc cgaatccgga atggcgaatc agatcaatgg
     3361 agaagccccg gggattatca gcggactcct ttcccggagc ggaggaaagc tcattcagtt
     3421 gtccacggac tatgtatttg acggtctttc ttccagaccg ctggatgaag aagccgttac
     3481 ggatcccgtg aatatttacg gggcgtccaa actgatggga gaacgggcgg cgatggcgaa
     3541 taatccgcag accctgatcc tccgcacttc ctggctgtat tctgcgttcg ggaataattt
     3601 tgtaaagacc atgatccgcc tgctgaaaac caaagaatcc atccgggtgg tgagcgatca
     3661 gaaaggatct cccacctacg ccgcggatct ggcgtccgcg atactgctca ttctttctgc
     3721 cgaaaaattt attcccggca tttatcacta tagtaatgaa ggagaaacca cctggtttga
     3781 ttttgcctca gagataaaag aaatgaccgg aagttcctgc accgtttccc cggtggaaag
     3841 ctccggttat ccgacagccg cccggaggcc ggcttattca ttgctggata aatcaaaaat
     3901 aaaaaagaac tacggcctga ccattcccgg ctggaaaacc agcctggccg cctgtcttga
     3961 aattctgcta agatctgaag tgtaataagg atcgataagg ttctttttat tttgcgaatt
     4021 tggcttgctt caggttttcc ggcttgagcc cgataaccgg tgtgtcgctt accccgtaca
     4081 ataattcttc attgaggtca agcacatctt tctgcaaacc gtaaacctcc tgtgtaagtc
     4141 ttctaacttt cttagtgcca agcagataac caattccaaa aataaaaaga ccagcgatcg
     4201 tcacttccag catattaagc tgaattgcct ggagtaaatc aataatggct tccatagttg
     4261 ttagttttta taggattgga tcaaacacat ctaaaagtcg atgaataacc atattcaccc
     4321 gattaaatgc acttaaaagt agattaacaa actcataagt acaacgtaga actcccatga
     4381 attatttggt ataaatacgg aattcccccg ccagtgcccg
//
LOCUS       DASYCU010000078         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2630429, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000078 DASYCU010000000
VERSION     DASYCU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630429"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VGM24_01535"
     CDS             complement(<1..295)
                     /locus_tag="VGM24_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY4154070.1"
                     /translation="MLNIESQQSKARAFRDLHSGKSLLILPNIWDPLGAALLEDLGYP
                     AIATASASIAYTHGMQDGENNKFGDLLLLLKKISASVSVPVTADIESGYAGTED"
     gene            456..2672
                     /locus_tag="VGM24_01540"
     CDS             456..2672
                     /locus_tag="VGM24_01540"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEY4154071.1"
                     /translation="MLKLLLTLLLASVTNLSALSQETDVFPPDSVKKEIKAIPISGSL
                     HIDGIMNEPEWSLAGPSPRFIQIEPAQGKPANFETLVKVLYNRQFLYVGVFAKDTLGK
                     KAIMATDFMRDFDFLRHDLVSLAFDGFNDRRNAMSSVTDAYGVQRDLLSFDDLYYDLD
                     WDGLWKVRTNRTDSGWTAEFAIPWKTLRYPKTADSIQSWGFNVYRNRRLTNEISALSE
                     FPRVFTSLRMNYAGRLTNLQPPPPKPNIRVQPYLLSSYDHYKNFDPPVQESQTHIKLG
                     GDLKWAINPNSVLDLTTNTDFAQADADLQVNNVSRFSVFFPEKRQFFLENASLFGIGI
                     GQSADAAGGTMRIQPFFSRTIGLDTNGSPIPIDAGARFVHRSSKLNYGAILIRQRGIP
                     DMPGTNFFVGRLSENFGTQNRIGLLSTIKNNRDGSNIGTTVDGFFRLSDAQSLNTMFM
                     HTYSTQTGKQGFSGFAQYYITTNHFKIWWTQSVVTRDFDPEMGFISRKDVIGTTPGIF
                     YYYRGKHLPLKKILLAWEPSISPEIYNQASTGKLIETSFTTYPVWLNFKSGAYFGYGF
                     IPTYQGLTEPFEPLGVKIAAGDYHYLRHQIWASTNPSKIVNVQLIYTWGPYFNGRLNS
                     ADWKFQFAPIPNISLTGEFNRNRFIGVGELKSYSTVDLYIIQGRFALTPRMQLIGFYQ
                     KNSENSSQNYNIRFSWEYSPLSYVYLIYNHVEFSNPLGKNPGEDHVIAKISYLKQF"
     gene            complement(2749..>4188)
                     /locus_tag="VGM24_01545"
     CDS             complement(2749..>4188)
                     /locus_tag="VGM24_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154072.1"
                     /translation="EEKPTYFLTPETPVSNLKDARAFTDASYQNLQGLLAGQSGSYGG
                     NVWNLMEFMTGKSNSDLGQTGFINFQNLSYNGTSFYVDTWWQQMYLGIGACNLAIEKV
                     PTIVAAGLTDTARTNMLAEAHTLRALYYFYLVRMYGAVPKVTETPKDLNLKLPRTPAK
                     DIYDSLIIPDLLLAEQSTLPWKDPNGRVSMGAVKSILADVYLTYAGAAINGGENFYAE
                     SAKRSLDVIQNGGYTLFPEYTDMIKPSNKNSGGFIFQVQYAASVPSTNPLTALTIPNY
                     SAISKYSDEYGSVYPTPQFVASFPSGDKRAQEKQFFYTHYPNINTGAIVNFKGPYIYK
                     WFDSVAVTSTVKSDLNYTLYRLADVMLMYAEASNRAEGGPNATAISYVNAIRARANLA
                     PIGALSEDAFEQEVWLQRYFELCFENKMWFDMLRTRKVHNDVTGNWDDFVGHTTVFGA
                     SFTEKQLLFPLPKQETDVNPNLLPNNPGF"
BASE COUNT         1014 a         1137 c          988 g         1049 t
ORIGIN      
        1 catcctccgt tccagcatat ccgctttcga tatccgccgt taccggcacg ctgacgctgg
       61 cgctgatctt cttcagcagg agcagcaggt cgccgaactt attattctct ccatcctgca
      121 taccgtgagt atacgcaatg gacgcactgg ccgtggcaat ggcgggatag cccaggtcct
      181 ccagcagggc ggctcccagg ggatcccaga tattcggcag gatcagtaag gatttgccgg
      241 aatgcaggtc ccggaatgcc ctggctttgc tttgctgcga ttctatattc agcattttta
      301 ttttttttat tatatacccg gatagctcta atccggtaat gcctgtacaa cggtccggac
      361 caaaattaat cattgccatt catggattcg ggttcagtcc tttttttgct taatttgagt
      421 ttcataaaag cgaacaggct tcgggtacca cgaccatgct gaaactactg cttacactat
      481 tgcttgcttc cgtaaccaac ttgtctgcac tctcccagga aacggatgta ttcccgccgg
      541 actccgttaa aaaagaaatt aaggccatcc cgatttccgg ctcccttcac attgatggaa
      601 taatgaatga accggaatgg tcactggcag gcccttcccc gcgttttatc cagatcgaac
      661 cggctcaggg taaaccggcg aattttgaaa ccctcgtgaa agtgctttac aaccgccagt
      721 tcctgtatgt cggcgttttt gccaaagaca ccctgggtaa aaaagcaatc atggccaccg
      781 atttcatgcg ggatttcgac ttcctgcggc acgacctggt ttcgctggct tttgacggat
      841 tcaatgaccg ccgaaatgcg atgtcctctg taaccgatgc gtacggcgtg cagcgcgacc
      901 tcttatcgtt cgacgatctc tattatgatc tcgactggga cgggctgtgg aaggtcagga
      961 ccaaccggac agattccgga tggaccgccg aatttgccat tccctggaag accctgcgat
     1021 atcccaaaac ggcagactcc atccagtcct ggggctttaa cgtttaccgc aaccggagac
     1081 tgaccaatga gatctccgca ctgtctgaat ttccacgggt ctttacctcg ctccggatga
     1141 attatgccgg ccggctgacc aacctgcaac cgcctccgcc caaacccaat atccgcgtcc
     1201 agccctacct gttaagctct tacgaccact ataaaaattt tgacccaccg gtacaggaat
     1261 cgcaaacgca tatcaaactc ggtggcgatc tcaaatgggc tatcaacccg aattccgtac
     1321 tggacctcac cacgaacacc gattttgccc aggccgatgc cgacctgcag gtgaacaacg
     1381 tgagccggtt ctctgttttc tttccggaaa aaaggcaatt ctttctggaa aatgcttccc
     1441 tgtttggcat cggcatcgga caaagcgcgg atgctgccgg aggaaccatg cgtatccagc
     1501 cttttttcag ccggaccatc ggcctggata caaacggcag cccgatcccg atcgacgccg
     1561 gggcccggtt tgtgcatcgc tcctccaaac tgaattacgg cgccatactg atccggcagc
     1621 ggggtatacc cgatatgccg ggaacgaatt tttttgttgg ccgcctgtct gaaaatttcg
     1681 gcacacaaaa ccggatcggg ttgctgtcaa ccatcaagaa caaccgggac gggtcgaata
     1741 tcggtacaac cgtcgatggt tttttccgat taagcgacgc acaatcgctg aacacgatgt
     1801 tcatgcatac ctattccact caaaccggca agcagggttt ctccggcttc gcccagtact
     1861 atatcaccac caatcatttt aagatctggt ggacccagtc tgtggtgacc cgggacttcg
     1921 atcccgaaat gggttttatt tcgaggaaag atgttatcgg aaccacgccg ggcatttttt
     1981 attattaccg cggaaagcac ctgcccttaa aaaaaatatt gcttgcctgg gagcccagca
     2041 tcagtccgga gatctataac caggcgtcta ccggcaaact gatcgaaacc agttttacga
     2101 cctatcccgt atggctgaat ttcaaaagcg gcgcctattt cggatatggt tttattccta
     2161 cttaccaggg attgaccgag ccctttgaac ccctcggcgt aaaaattgcc gcgggggact
     2221 atcattatct ccggcaccag atctgggcca gcaccaatcc atccaaaatc gtcaacgtgc
     2281 agctgatcta tacctgggga ccgtatttca acggccgcct gaattcagcc gactggaaat
     2341 ttcagtttgc tcccatcccc aatatttccc taacggggga gtttaaccga aaccggttta
     2401 tcggagtagg cgaacttaaa tcctattcga ccgttgacct gtatatcata cagggacgtt
     2461 tcgcgcttac cccccggatg cagctgatcg gcttctatca gaaaaattcc gaaaacagtt
     2521 cacagaatta caacatccgc ttttcctggg agtattcccc gctttcctat gtatacctca
     2581 tatacaatca tgtcgaattc agcaacccgc tgggaaagaa tccgggagaa gatcatgtga
     2641 ttgcgaaaat cagttacctg aagcagtttt aaggatatcc gttccgggac ctgcaaaaaa
     2701 attgcccggc actatccgcc gggcaatcca ttcaaaaagc cttcggaatc aaaagcccgg
     2761 attattggga agaaggttcg gattaacatc tgtttcctgt ttcggaagcg gaaacagcaa
     2821 ctgcttttca gtaaagctgg cgccgaatac cgtggtatgc ccgacaaaat catcccagtt
     2881 gccggtaaca tcattatgca ccttcctcgt ccgcagcatg tcgaaccaca ttttgttctc
     2941 aaagcacagt tcaaaataac gctgcaacca cacttcctgt tcaaaagcgt cctcgctcaa
     3001 agcccctatc ggagccaggt ttgccctggc ccggatagca ttgacataac tgatagctgt
     3061 tgcgtttggc cctccttccg cgcggttgga cgcttccgca tacatcagca tcacatccgc
     3121 caaccggtaa agcgtatagt tcagatctga ttttaccgta ctggtcacgg caaccgaatc
     3181 aaaccatttg taaatatatg gccctttgaa attgacgatt gccccggtgt taatgttggg
     3241 atagtgggta taaaagaact gtttttcctg ggctctctta tcacctgaag gaaaagaggc
     3301 cacaaattgc ggggtcgggt aaacagatcc gtattcatcc gaatatttgg atatcgccga
     3361 atagttcgga atcgtaaggg cggtaagcgg attggtggac ggaacagacg ccgcatattg
     3421 cacctggaaa atgaacccac cgctgttttt attggaaggt ttgatcatat ccgtatattc
     3481 cgggaacagg gtataccctc cgttctggat cacatccagg gatcgtttgg cagactccgc
     3541 gtaaaagttt tctcctccgt tgatggccgc cccggcatag gtgagataca catccgccag
     3601 aatggatttt acagcgccca tagaaacccg gccgttcgga tccttccagg gcagcgtgga
     3661 ttgttcagcc agcagcaggt cgggaatgat caggctgtcg tagatatcct ttgccggggt
     3721 ccgcgggagt ttcagattga ggtccttggg agtttccgtt actttcggaa ccgcaccgta
     3781 cattctgacc agataaaaat aatacagggc cctcagcgta tgcgcttccg ccagcatatt
     3841 ggttcttgcc gtatcggtca gcccggctgc cacaatcgtg ggcacttttt caatggccag
     3901 attgcatgcc ccaatgccca gatacatctg ctgccaccag gtatccacat aaaaggaagt
     3961 gccgttatag gagagattct gaaaatttat gaagccggtc tgtccaaggt cgctgttgga
     4021 tttgccggtc atgaattcca tcaggttcca tacgtttcca ccataggagc cggactgtcc
     4081 tgccagcagt ccctggagat tctgataaga tgcatcggtg aatgcccggg cgtctttcag
     4141 gttactgacc ggtgtttccg gagtcagaaa ataggtgggc ttttcttc
//
LOCUS       DASYCU010000079         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3066779, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000079 DASYCU010000000
VERSION     DASYCU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3066779"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VGM24_01550"
     CDS             <1..308
                     /locus_tag="VGM24_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154073.1"
                     /translation="FEPTVPHIINGQAAGKGIAIFSTPAPDFELGRIHLIRGQELGLV
                     SQSVQLFIVVEGSVEVLEKEGGEFARKKGESWVSFDGAGSSLKALDDTVIYRAAIPD"
     gene            346..921
                     /locus_tag="VGM24_01555"
     CDS             346..921
                     /locus_tag="VGM24_01555"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HEY4154074.1"
                     /translation="MKHTKTLIWSFILLVFIAAVYRIIPGRPYGFAPQWAMAVFAGAV
                     IKNRKWALIMPVLSMFISDLFYQLLYMGGLTEMAGFYDGQWQNYILFALLVFIGFAIK
                     KYNVLPIAAASLAAPTVYFLLSNFIVWSGWDGTRGLGRPKTWSGLMMCYTDGIPFYIN
                     SLLATVIFSAILFGSFLLIMKSKQRLSPTGH"
     gene            complement(918..1388)
                     /gene="rnhA"
                     /locus_tag="VGM24_01560"
     CDS             complement(918..1388)
                     /gene="rnhA"
                     /locus_tag="VGM24_01560"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY4154075.1"
                     /translation="MHQDPIIIYTDGASRGNPGPGGYGTILQWKGHVKELSAGYQKTT
                     NNRMELMAVIAGLEALKKEGMKVQIYSDSQYVVKSVEQGWLKNWIATDFKGGKKNKDL
                     WLRYAGLAKKHHIRFHWVKGHASNPHNNRCDELATQAADGKRLLPDEGFTGTDG"
     gene            complement(1399..3030)
                     /locus_tag="VGM24_01565"
     CDS             complement(1399..3030)
                     /locus_tag="VGM24_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY4154076.1"
                     /translation="MDLDFNRNEDKMKQALSLLRNRFQKIVLGGGVASIDKQHDKNKL
                     TARERIAYLIDDHSSFTEIAAFAGYEMYEEYGGCPAGGTVAGIGYVSGRQVVILANDQ
                     TVKAGAWFPVTGKKNLRLQEIAMENHLPVIYIVDSAGVFLPMQDEIFPDKEHFGRIFR
                     NNARMSAMGITQLSAVMGPCVAGGAYLPIMSDETLMVEGKGSIFLAGPYLVKAAVGET
                     TDSETLGGAVTHTAISGIADYKFKTEEECLDQIKKIIGTLGHQIQSAGFDRINPAAPS
                     KDGKDIYGLIPEDPTKPYDMLEIISRIVDAPGIQQFKEDYGKTIICGYARLEGWAVGI
                     VANQRIVVKTKKGEVQMGGVIYNDSADKAARFIMNCNQKKIPLIFLQDVTGFMVGSRS
                     EHSGIIKDGAKMVNAVANSIVPKFTVIIGNSYGAGNYAMCGKAYDPRLIVAWPSARIA
                     VMGGDQAAKTLLQIQVSSLQSRGQPVDPAEEKKLLATIRERYEKQTSPYYAAARLWVD
                     ALIDPLDTRQLLGEALKAANHNPRFSEFKTGVFQV"
     gene            3072..>3384
                     /locus_tag="VGM24_01570"
     CDS             3072..>3384
                     /locus_tag="VGM24_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HEY4154077.1"
                     /translation="MQWKRFAPWLLPVIPAAGIKIFTFFPDAIEKYYSNGLYPFLARL
                     LRFAFGWIPFSAGDVLYGILFVYGLVSMGHFIKKAYQRRLHREYLFYCAGRLASGVLW
                     IY"
BASE COUNT          701 a          884 c          847 g          952 t
ORIGIN      
        1 aatttgaacc aaccgttcct catattatca acgggcaggc tgccggaaag ggaatcgcca
       61 ttttttccac ccccgcgccg gacttcgaac tgggacggat tcatctgatc cgcggacagg
      121 aactgggcct ggtctcccaa tccgtacaat tatttattgt agtggaaggg tcggtggaag
      181 tgttggagaa ggagggcggc gaatttgcca gaaaaaaggg cgaatcctgg gtaagtttcg
      241 atggggccgg gagcagcctg aaggctttgg atgatacggt catttatcgg gcggcgatac
      301 cggattaacg aaaatcagtt aattttacat ttctaaattc aaatgatgaa gcatacgaaa
      361 acactgatct ggtcctttat cctgctggta tttattgccg ctgtttaccg gattataccc
      421 ggccgccctt atggatttgc cccgcagtgg gcgatggctg tttttgccgg ggcggtgatc
      481 aaaaaccgga aatgggccct gatcatgccc gtgctttcca tgtttatatc cgatttgttt
      541 tatcagttgc tttatatggg cggtctcacg gaaatggccg gtttttacga tggccagtgg
      601 cagaattaca ttctgtttgc cctgctggtg tttatcgggt ttgccattaa aaaatacaat
      661 gtgcttccta tcgcggcagc ctcccttgcc gctccgacgg tctatttcct gctttccaat
      721 tttattgtat ggtcgggatg ggatggcacg cgcggactgg gccggcccaa aacctggagc
      781 ggactgatga tgtgttatac ggacggaatt cccttttaca taaatagcct gctggcgacc
      841 gttatcttct ccgctattct tttcgggagt ttcctgttga tcatgaaaag caagcagcgc
      901 ctttctccaa ccggtcatta accgtccgtt ccggtaaacc cttcatccgg caatagccgc
      961 ttcccgtcgg ctgcctgcgt agccagttca tcgcagcgat tattatgggg gttcgatgca
     1021 tgccctttca cccaatgaaa gcggatatga tgttttttgg cgagtccggc ataacgcagc
     1081 cagaggtctt tgtttttctt tccgccttta aaatccgttg cgatccagtt tttcagccag
     1141 ccctgttcga ccgatttcac cacatactga ctgtcggaat aaatctgcac tttcataccc
     1201 tcttttttca atgcttccag cccggcaatc acggccatca attccatgcg gttattggtg
     1261 gtcttctgat atccggctga aagctctttt acatgaccct tccattggag aatggtgccg
     1321 tagcctccgg gaccgggatt gccccttgaa gcgccatctg tatatatgat tatgggatcc
     1381 tgatgcatag cccgccggtt aaacctggaa aaccccggtt ttgaattcgc tgaaacgcgg
     1441 attgtggttt gccgctttaa gggcttcccc aagcagttgc ctggtatcga gcggatcgat
     1501 cagcgcatcc acccagaggc gggccgccgc ataatagggg ctggtttgct tttcataacg
     1561 ttcccggata gtcgccaata gttttttctc ttcggccggg tcaaccggct ggcccctgga
     1621 ttgcagggaa ctgacctgga tctgcaaaag ggttttcgcc gcctggtccc cgcccatcac
     1681 cgcgatccgc gcgctgggcc aggcaactat caaccgggga tcataggctt ttccgcacat
     1741 ggcatagtta cccgctccgt acgagtttcc gatgataacg gtaaatttcg gcacgattga
     1801 attcgcaacg gcattcacca tctttgcccc gtctttgatg atgccggaat gttcgcttct
     1861 cgaacccacc ataaacccgg taacgtcctg gagaaagatc aggggaattt ttttctggtt
     1921 gcagttcata ataaagcggg cggctttatc cgcactgtcg ttgtagatca ctccgcccat
     1981 ctgcacttct cctttctttg ttttcaccac gatgcgctgg ttggccacaa tgcccaccgc
     2041 ccatccttcc agtctggcgt acccgcagat gatggttttt ccatagtctt ccttgaactg
     2101 ctgaataccg ggagcatcca cgatccggct gatgatttcc agcatatcat agggtttcgt
     2161 cgggtcttcc gggattagcc cgtaaatgtc ctttccgtct tttgaaggag ctgccggatt
     2221 gatccggtca aatccggcag actgtatctg gtggccgagg gtgccgatga tcttttttat
     2281 ctggtccagg cattcctctt ccgttttaaa tttataatcg gcgatgccgg aaatggccgt
     2341 gtgcgtgaca gcgccgccaa gggtttctga gtcggttgtt tctccgacgg cagccttgac
     2401 caggtaaggt ccggctaaaa atattgagcc cttgccttcc accatcaggg tttcgtcgct
     2461 catgatgggc aggtaggcac ctcccgccac gcagggaccc ataaccgcag atagctgggt
     2521 gatccccatg gcactcatcc gggcattgtt ccggaagatg cggccgaaat gttccttgtc
     2581 cggaaaaatc tcgtcctgca tcggcagaaa taccccggcg ctgtccacga tgtaaatgac
     2641 gggcagatga ttttccatcg caatctcctg caaacgaaga ttttttttcc ccgttaccgg
     2701 aaaccaggca cctgccttca ccgtttgatc gttggccagg atcacgacct gccggccgct
     2761 cacataaccg atgccggcca ccgttccgcc cgcagggcag ccaccgtatt cttcatacat
     2821 ttcatatccg gcaaaagccg cgatttccgt aaaggaagaa tgatcgtcaa tcaggtaggc
     2881 aatccgttcc ctggcggtga gcttattttt atcgtgttgc ttatcaatgg atgcgacccc
     2941 tccgccgagc acaatttttt gaaatcggtt ccggagcagg ctgagggcct gcttcatctt
     3001 atcttcattc cggttaaaat caagatccat ggtgctggta tatgctgcaa taataatgta
     3061 tttttaaagg tatgcaatgg aaacgctttg ctccctggtt gcttccggtt attcctgcag
     3121 ccggcattaa aatttttact ttttttccgg acgccatcga aaaatattat tcaaatggcc
     3181 tgtatccttt tctcgcgcgg ctgctgcgct ttgcgttcgg atggatcccg tttagcgccg
     3241 gcgatgtact gtatgggatt ttatttgttt acggactggt ttcaatgggt cattttataa
     3301 aaaaagcata ccagcggcga ttgcaccggg aatatctttt ttattgtgcc gggcggctgg
     3361 cttcaggggt cctgtggatt tatg
//
LOCUS       DASYCU010000080         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1471665, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000080 DASYCU010000000
VERSION     DASYCU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471665"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VGM24_01575"
     CDS             <1..1637
                     /locus_tag="VGM24_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4154078.1"
                     /translation="IGMKKSLHLQPIAEIHTTAGYDAEAGKTVSSTFLHLLILIAILI
                     QVIACINFMNLSTARASKRAKEVGIRKVAGAGRKSLIGQFLGESFLLVLLSVLIAVPM
                     LAWTMPLLNRITETDIQLSLLTNGNLWILLAAIILVTGIAAGSYPAFYLSAFQTIKVI
                     KGNFTNHISATGIRRSLVVFQFVLSIMLITSIIIIYSQLEFIKNKDLGFEQNQQLIFS
                     FHTGDTKNKMEAFESDLRSLPEVKTASKANNFPGAVSYNDWQVYLAGGNLATATDQQN
                     ISSDENIVKALGVTLISGRDFHLHDSGAVIINETLMKRLNLKPETAPGTRLFTGDGES
                     YGIAGVMKDFNYNSLRDLVYPFMFIYNPGRDDINHLIVNIRSTNYSALMGKMGKIWHK
                     DLPSTPFVVKFLDEEVQKQYETEISMSRIINSFTLMAILISCLGLFGLATFSAEQRSK
                     EIGVRKVLGASVTGIVGLLSGDFLKLVLIAFLIATPVSGWVMQEWLQRFAYRISVSWW
                     MFGLAGGLAVFIALFTISFQAIRAALANPVKSLRTE"
     gene            1728..2744
                     /locus_tag="VGM24_01580"
     CDS             1728..2744
                     /locus_tag="VGM24_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY4154079.1"
                     /translation="MKFQIGDKVTVLHSNEEAEVVEMINDKMVMVDVRGVKFPAYIDQ
                     LDFPYFRRFTEKKIFPVKKEKKYVDQVPVDRTPAKNNPLDGVWLSFLPVSDTDEFGDE
                     VVESLKIHLVNHSAAALHFKYRLLYFGKMDFELENEVSPGKDFYLHDMAFATLNDSPS
                     FDLDFSLAVPDKKKAPHFERSLKLKPRQVFMRIEEVRRKGGASFTYKLFDVYPDKMPE
                     EDKPDLNLLAGKGYKMYEASQSRQHLEPARYEVDLHIEKLTDSWSHLSNFEKLTLQLN
                     TFEKYLDLAIAHHQHNLIVIHGVGSGKLKDEIHEILKSRRGVRSFINQYDPRYGYGAT
                     EIFL"
     gene            2756..3143
                     /gene="rnpB"
                     /locus_tag="VGM24_01585"
     ncRNA           2756..3143
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGM24_01585"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3174..4124)
                     /gene="plsX"
                     /locus_tag="VGM24_01590"
     CDS             complement(3174..4124)
                     /gene="plsX"
                     /locus_tag="VGM24_01590"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY4154080.1"
                     /translation="MNIAFDMMGGDFAPGEVLKGIRDYLADHSAQAFLLLIGDEAVLK
                     PLLDEYPLSPKTYKIVHASEVIGMHEHPLKALKEKQQSSIAIGFHLLSAGKADAFISA
                     GNTGAMLIGSLYTIKPIEGIQRPTIATIVPRENGKTGLLLDVGANADCKAEHLNQFAV
                     LGSLYAQHILGIDNPRVGLINIGEEEGKGNVLAQATYPLLKTNPQINFIGNIEGRDIF
                     NDSADVMVCEGFTGNIILKLAESLHEVIGRQQIKNEYFDRFNFEDYGGTPVLGVNKPV
                     IIGHGISHAKAYRNMLLLAEKMIESKLLEKMSESFAAPDA"
     gene            complement(4161..4352)
                     /gene="rpmF"
                     /locus_tag="VGM24_01595"
     CDS             complement(4161..4352)
                     /gene="rpmF"
                     /locus_tag="VGM24_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY4154081.1"
                     /translation="MPNPKRRHSQQRSAKRRTHYKATKVTLSKDSTTGELHVRHRAHV
                     SEGKLFYKGKVVAEKSPVK"
     gene            complement(4382..>4919)
                     /locus_tag="VGM24_01600"
     CDS             complement(4382..>4919)
                     /locus_tag="VGM24_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY4154082.1"
                     /translation="SRREFEIAFVGLKPGVHEFEYQITDRFFEAYQQQDFEHCDVQVK
                     LFLEKNQGFMILRFEVVGKVDVQCDRCGNPLPLDLWDEFNVLVKLVEDPEIMNQEEED
                     PDVYYISRTESHLHLADWIYEFINLSIPVQRMCGEDEIGGPNCNREVLEMLKKLDSGT
                     GNESNPLWKGLEKFRTPE"
BASE COUNT         1175 a         1217 c         1281 g         1246 t
ORIGIN      
        1 cgattggcat gaagaaatca ctccatcttc aaccgattgc tgaaattcat accacggcag
       61 gatacgatgc ggaagcaggg aaaacggtga gcagcacctt cctgcatctc ctgatcctga
      121 tcgccatcct gatccaggtg attgcctgca tcaatttcat gaacctttcc acggcgaggg
      181 cctcaaagcg ggcaaaggaa gtggggatcc gcaaagtagc cggtgcgggc cgcaaaagcc
      241 tgatcggaca attcctgggt gaatcctttt tgctggtcct gttaagcgtg ctgattgccg
      301 tgcctatgct ggcctggacg atgcccctgc ttaaccggat cacggaaacg gatatccaat
      361 tatcgctcct gaccaacggg aacctgtgga tcctgctcgc agctatcatc ctggtcaccg
      421 ggatcgccgc gggcagttac ccggcttttt atctttccgc gtttcagacc ataaaagtga
      481 tcaaagggaa tttcacaaat catatttccg ccaccggcat tcgccgttct ctggtggtat
      541 tccagtttgt gttatccatt atgctgatca cgtccatcat tatcatatac agccagctgg
      601 aattcatcaa aaataaggac ctgggttttg aacagaacca gcagctcatt ttcagcttcc
      661 ataccggcga taccaaaaac aaaatggagg cttttgaatc cgatctgcgg agcttaccgg
      721 aagtgaagac ggcgagtaaa gcaaataatt ttcccggggc ggtttcctat aacgactggc
      781 aggtctatct ggccggggga aacctggcca cggccacaga ccagcagaat atttcttccg
      841 acgagaacat tgtaaaagcg cttggcgtta ccctgatcag cggaagggat tttcatctgc
      901 atgattcggg agcagtcatt attaacgaaa ccctcatgaa acgcctgaac ctgaagccgg
      961 agacggcgcc gggcacccgc ttatttaccg gtgatggcga atcctacggg attgcgggag
     1021 ttatgaaaga ttttaattac aattcactcc gggatctggt ttatccgttc atgtttatct
     1081 ataatccggg ccgcgacgat atcaatcatt taattgtgaa catcagaagc accaattact
     1141 ctgcacttat gggaaaaatg ggaaagatct ggcacaaaga tctgccgtcc accccgtttg
     1201 tggttaagtt cctcgatgaa gaagttcaga aacaatacga aacggagatc agcatgtccc
     1261 gcatcatcaa ttccttcacg ctgatggcga tcctgatttc ctgcctgggg ttgttcggcc
     1321 tggccacctt cagtgcggag cagagaagta aagaaattgg cgtgcggaag gtattgggcg
     1381 cgagcgtgac cggcatcgtc ggattgctgt ccggggattt tctaaaactg gttttgatcg
     1441 cttttctgat tgcgacgcct gtttcggggt gggtgatgca ggagtggtta caaagatttg
     1501 cctatcgcat ttcagtcagt tggtggatgt ttggactggc cggcgggctg gctgttttca
     1561 tcgccttgtt caccatcagc tttcaggcga tccgggccgc cctggcgaat ccagtgaaga
     1621 gtttgcgtac cgaataggaa gactcccggt taaaaaggat tgaagaattg tttatccggt
     1681 tggatccggc ggacagtatt cggtcaactc ttttgtaact ttgcccaatg aagtttcaga
     1741 tcggcgacaa ggttacggtt ctccattcca atgaagaagc ggaagtggtg gaaatgatca
     1801 atgacaagat ggtgatggta gatgtgcggg gcgtgaaatt tccggcctat atcgatcagc
     1861 tggacttccc ttatttcagg agatttacgg agaagaagat tttcccggtg aagaaagaga
     1921 aaaagtatgt ggatcaggtg ccggttgacc ggacaccggc aaagaacaac cccctggacg
     1981 gggtatggct cagctttctt ccggtttccg atacggatga gttcggggat gaggtggtgg
     2041 aaagcctgaa gatccacctg gtcaatcatt ccgccgctgc ccttcatttc aaataccggc
     2101 tcctgtattt tggtaagatg gattttgaac tggagaatga agtatctccc ggaaaagatt
     2161 tttacctgca cgatatggct tttgccaccc tgaacgacag cccttccttc gacctggatt
     2221 tcagtctcgc cgtgccggat aagaagaaag cgcctcactt tgaacgttcc ctcaagctaa
     2281 agcccaggca ggtctttatg cgcatcgaag aggtccgcag aaaaggggga gccagtttta
     2341 cgtataaact tttcgatgtc tatcccgata agatgccgga agaggataag ccggatctta
     2401 atctgctggc gggaaaggga tataaaatgt atgaggcatc ccaaagccgg cagcatctgg
     2461 agccggccag gtatgaagtc gatctgcaca ttgaaaagct cacggattcc tggagtcatc
     2521 tcagcaattt tgagaagctc acgcttcagc tcaatacatt tgaaaagtac ctggacctgg
     2581 ccattgcgca tcaccagcac aacctcatcg tcattcatgg agtcgggagc ggcaaactca
     2641 aagacgagat ccatgagatc ctgaagtcgc gtcgtggcgt gcgctctttc atcaaccagt
     2701 acgatccgcg ctacggatac ggcgctacgg agatatttct ctaatttcgc ggcaagctac
     2761 cggccgggcc atcgctccgg aaacggggag gaaagtccgg acagcacagg gcaatgcacc
     2821 ggttaagcgc cggggtcatc attgcgaggg ctaatgatac agatagtgcc acagaaaata
     2881 accgcctctc cggaggtaag ggtgaaaacg tagggtaaga gcctacggcg gcgttcagta
     2941 atgggcgcgg cgggtaaacc ttgtatgctg taatgccatg tatgttctgt tgattgagcc
     3001 ggtccggctc aaaaggtgaa cggtccgttc acatcgccgc agggcgtaac aggacgggta
     3061 ggcagcaaga tccgttcagt aatgggcggg caagataaat gatggcagag aaacagaatc
     3121 cggcttatga gccggtagct tttttttcct acctgatata atgggaaacc gggttaagcg
     3181 tcaggagcag caaagctttc actcattttt tccagcagct tcgattcaat catcttttca
     3241 gccagcagca gcatgttccg gtatgctttg gcgtgagaga tgccatgacc gatgatgacg
     3301 ggtttgttta cgcccagcac gggcgttccc ccgtaatcct cgaagttgaa gcggtcgaaa
     3361 tattcattct ttatctgctg gcggccgatg acctcgtgga gagattctgc cagtttcagg
     3421 atgatattgc cggtaaatcc ttcgcagacc atcacgtcgg ccgaatcatt gaagatatcg
     3481 cggccctcta tatttccgat gaaattgatc tggggattgg ttttgagcag gggataggtg
     3541 gcctgtgcga gtacattgcc ctttccttct tcttccccga tattgatgag ccctacccgg
     3601 ggattgtcga ttcccagaat atgctgggca taaagcgatc cgagtaccgc aaactggttg
     3661 aggtgctccg ccttgcaatc cgcattggcg cccacatcca ggagcaggcc ggtctttccg
     3721 ttttcgcggg gaacaatagt cgcaatggtg gggcgttgaa taccctcgat cggcttgatc
     3781 gtatagagtg aaccgatcag catggcgccg gtgtttccgg cgctgatgaa ggcatctgcc
     3841 ttcccggcag agagcagatg aaaaccgatg gcgatcgaag actgctgctt ttccttgagc
     3901 gctttcaacg gatgttcgtg catgccgatc acttcggatg catgtactat cttatacgtc
     3961 ttgggggaaa gcgggtattc gtccagcagg ggtttcagga cggcttcgtc accgataagc
     4021 aggaggaatg cctgagcgga atggtcggcc agatagtccc ggatcccttt aagcacttct
     4081 ccgggtgcaa agtctccgcc catcatatcg aatgcaatat tcatccaggt tagatctaca
     4141 ttaaatatgc caaacgaatt ctactttacc ggcgattttt cagccactac tttaccctta
     4201 taaaataatt ttccctcgct cacatgggcg cggtggcgaa catgcagttc tccggtagtg
     4261 ctatccttgc tcagggtaac cttggtcgct ttgtaatgtg ttcttctctt ggcgcttctt
     4321 tgctgcgaat gtctccgttt gggatttggc atgactcttc aatttatttt aacctaaata
     4381 tttattcggg cgtccggaac ttttccaacc ctttccagag cggattcgat tcgttgccgg
     4441 taccggaatc cagttttttt aacatctcca gcacttcgcg gttacagttc ggaccgccga
     4501 tctcgtcttc cccgcacatc cgctgaaccg ggatactcag gttgatgaac tcgtagatcc
     4561 agtcggccag gtgaagatgg ctttccgttc gcgagatata ataaacgtcc ggatcctctt
     4621 cctcctggtt cataatctcc gggtcctcca ccagcttgac cagcacgttg aattcatccc
     4681 agaggtcaag gggaagcggg ttcccgcaac ggtcacactg aacgtccact tttccgacca
     4741 cctcaaaccg gagtatcata aagccctggt tcttttccag gaacaatttc acctgtacgt
     4801 cgcagtgttc aaaatcctgc tgctggtatg cttcaaagaa cctgtccgtg atctgatatt
     4861 caaactcatg aacgccgggc ttgagtccca caaacgcaat ttcgaattct ctgcggctg
//
LOCUS       DASYCU010000081         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1180735, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000081 DASYCU010000000
VERSION     DASYCU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180735"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1663
                     /locus_tag="VGM24_01605"
     CDS             257..1663
                     /locus_tag="VGM24_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4154083.1"
                     /translation="MYIQQLYTGCLSEAAYYIEDQGEAAIIDPLRDIEEYLLIAKERK
                     ATIKYIFETHFHADFVSGHLDLAQASGAPIIYGPQTETAFPIYKATDGEKFKLGDLTI
                     EVLHTPGHTVESSCYLLRDANGKAHAIFTGDTLFVGDVGRPDLSSGDLDKEELAGMLY
                     DSLQEKIKPLADDVIIYPAHGQGSSCGKNIGPDTYSTVGEERQTNYALKTQTREEFIK
                     AVTEGLNAAPQYFSINARINKEGYESLDGVMESALNPLSLEAFKVLIKDDDTIILDTR
                     NSADFTDGYIPGSISIGLDGRFAEWAGSILPFDKPIVLVTEPGKEKESAIRLARVGFD
                     QMQGFLNGSFETWKSAGETIDLVINVEPDELAMDLRFDPKLLVVDVRREAEFGDGHLI
                     DARNIPLETLTDPGTMADFNDTDNIYLHCTGGYRSVIAASLFKRQGIHNLRNIIGGYE
                     AIKGLEGVDVVKENSVLN"
     gene            complement(1660..2550)
                     /locus_tag="VGM24_01610"
     CDS             complement(1660..2550)
                     /locus_tag="VGM24_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4154084.1"
                     /translation="MYYVVYSICYLFSLLPLWALYGLSDLLMIFLYYIVPYRKKLVFS
                     NLAIAFPEKTEMERKRIAKDFYRNFIDHFLETLKLLSANKKFLLNHFTIDNPELFEQY
                     FLQGRRCQLHLGHSFNWEWANNSFPLVSEYPFIVIYMPISNKIFNRLFNHLRTRTGTI
                     LVAATDARRQMLAYRKLPYMLTLVADQAPGSPENAYWLNFFGKPAPFVRSPERGARAG
                     NTPAIFVHFYKPSRGKYRVKLFLGSDNPAGLPEGALTRKYVEFLEWSIGEQPALYLWT
                     HNRYKYGWKPEYESLMVDGR"
     gene            2664..>3257
                     /locus_tag="VGM24_01615"
     CDS             2664..>3257
                     /locus_tag="VGM24_01615"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4154085.1"
                     /translation="MKDKISLAEYYPQIDSVRAIAVLFVLFHHYLSERITSLLPLGSF
                     GVDIFFTLSGFLITGILISYRSIKPVGAAIRKFYFRRILRIFPIYYLYILIAAIIFGH
                     EISRTIILWASVYGLNFYIINHGLLPSYVFAHFWSLAVEEQFYLVWPFLILLFPFRQL
                     RYLIIFAIFISIGFSYYHISRPIMAYHPIACMQALAMG"
BASE COUNT          847 a          783 c          815 g          812 t
ORIGIN      
        1 ctatccttcg ccgtgccggt atcgtaaaaa ccgaacgcga tggcaagttc atccattata
       61 ccataaatcc gcaccggatt gcggatatta tgaagacggt gcagttgttg attgtttaag
      121 agcccttagg aaaggttgac cgttgacggt tgaccgttga ccgacttcag gtgcgggttc
      181 gcaataaaag tccgtcaacc gtcaaccgcc atccgtcaac aattaaatcg gtaaatttgt
      241 acctcaatca actcccatgt acattcagca actctacacc ggatgcctga gcgaggcggc
      301 ttattatatt gaggatcagg gagaggcggc cataatagac cctctgcggg atattgagga
      361 gtatctgctg attgcaaagg agcggaaagc cactattaaa tatatattcg agacccattt
      421 tcacgcagat tttgtgagcg gacatcttga tctcgcacag gcttccgggg cgccgattat
      481 ttatggccct cagaccgaaa ccgcattccc gatttataag gcaaccgacg gggaaaaatt
      541 caaactgggc gacctgacca tcgaagtatt gcataccccc ggacataccg tggaatcttc
      601 ctgctatttg ctgcgggatg ctaacggcaa agcccatgcg atatttaccg gcgacaccct
      661 ttttgtcgga gatgtgggca ggcccgacct cagcagcggc gatctggaca aggaggaact
      721 ggccggcatg ctctatgatt cgctgcagga aaagatcaaa ccactggcgg atgacgtgat
      781 catttaccct gcacacggcc agggcagcag ttgcgggaaa aatatcggcc cggataccta
      841 cagcacggtc ggagaagaac ggcaaaccaa ttacgccctg aaaacccaga ccagggagga
      901 gttcatcaaa gcagttaccg aaggacttaa tgctgcccct caatattttt ccatcaatgc
      961 ccggatcaac aaagaaggat acgaaagcct ggacggggtc atggaatcgg ccctgaatcc
     1021 cttgtctctt gaagcattca aggtcctcat taaagacgac gataccatca ttctggatac
     1081 ccggaattcc gcggatttta cagacggtta tatacccgga tccatcagca tcggactgga
     1141 tggaagattt gccgaatggg caggcagtat tcttcccttc gacaaaccca tcgtactcgt
     1201 tacggagccc ggcaaggaaa aagaaagcgc catccggctt gcccgtgttg gttttgacca
     1261 gatgcaggga tttttaaacg ggagtttcga aacctggaaa tcagccggtg aaaccatcga
     1321 tctggtcatc aacgtggagc cggatgaact ggcgatggac cttcgcttcg accccaaact
     1381 gctggtggtg gacgtgcgcc gcgaagccga attcggcgac ggccacctga tcgacgcccg
     1441 caatatcccg ctggaaaccc tcacagatcc aggcacgatg gccgatttca acgataccga
     1501 caacatctat cttcattgca ccggcggcta ccgaagcgtc atcgcggctt ccctgttcaa
     1561 gcgccagggc attcataatc tgcgaaatat tattggagga tatgaggcca ttaaggggct
     1621 ggagggagtg gatgtggtga aggagaacag tgtattaaat tagcgaccat ccaccatcaa
     1681 cgattcatac tcgggcttcc acccatactt atacctgttg tgcgtccaca aatacaaagc
     1741 gggctgctcg ccgatgctcc attccagaaa ttccacgtat ttgcgggtaa gcgcgccttc
     1801 ggggaggccg gcagggttgt cgctgcccaa aaataatttc acccgatatt ttccgcggga
     1861 gggtttataa aaatggacaa agattgcggg ggtatttccc gcccgggctc cgcgttccgg
     1921 tgaccggaca aagggggcgg gctttccgaa gaaatttagc cagtacgcgt tttccggaga
     1981 gccgggggcc tgatctgcaa ccagggtaag catgtacgga agtttacggt aagccagcat
     2041 ttgtctgcgg gcatcggtcg ccgccaccag gatggttccg gtgcgggtac gcagatggtt
     2101 aaaaagccgg ttaaaaattt tattgctgat gggcatgtag atcacgatga atggatattc
     2161 tgaaaccagg gggaatgaat tatttgccca ctcccagtta aaggaatgac ccaggtgcag
     2221 ctggcagcgc cggccctgta gaaaatattg ttcaaataat tccgggttgt cgatggtaaa
     2281 atgattcagg agaaattttt tattggcgga aagcagtttc aatgtttcaa gaaaatggtc
     2341 aatgaaattc cggtaaaaat ctttcgcgat tcttttcctc tccatctccg ttttttcggg
     2401 aaaagcgatc gccaggttgg aaaaaacgag ttttttccgg tagggcacga tataataaag
     2461 aaagatcatg agcaagtcgg acaggccata aagtgcccac agcggaagca gggaaaaaag
     2521 ataacagatg gaatagacta cataatacat gcgggcacac tatgccgcaa ataaccgttg
     2581 attttctgga aacgcaaaaa gaggaatggg agcgccgggc ctgatgaacg gcataatttt
     2641 aagttttctt aactttcctg ctgatgaaag acaaaatatc attagcggaa tactatcctc
     2701 aaatagattc tgtccgtgcc atcgccgtgc tttttgtatt gtttcatcat tatctttctg
     2761 aaaggataac cagcttgttg cccctgggct cattcggagt ggatattttc tttacgctca
     2821 gcgggtttct gatcaccggc attctgattt cttaccggtc aatcaaaccg gttggcgcgg
     2881 caatcaggaa attttacttc aggaggatcc tgcgaatttt tccgatttat tacctgtata
     2941 ttctgattgc cgcaataata ttcggacatg aaatttcacg gaccattatc ctgtgggcat
     3001 cggtttacgg gctgaatttc tacatcatca atcatggcct gttgccgagc tatgtttttg
     3061 cccatttctg gtccctggcc gtggaagaac aattctacct ggtctggccc tttcttattt
     3121 tgctttttcc ctttagacag ctcagatatc ttatcatatt tgctattttt atcagcatcg
     3181 gatttagcta ttatcatatc tccaggccga ttatggccta ccatccgatt gcctgcatgc
     3241 aggctctggc gatgggt
//
LOCUS       DASYCU010000082         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3212052, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000082 DASYCU010000000
VERSION     DASYCU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212052"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..906)
                     /gene="kdsA"
                     /locus_tag="VGM24_01620"
     CDS             complement(85..906)
                     /gene="kdsA"
                     /locus_tag="VGM24_01620"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY4154086.1"
                     /translation="MKAFLQELFRGQQYDEKNFFLIAGPCVVENEELLDTVASKVSVL
                     CANLGIPYIFKASYRKANRTSSSSFAGIGDEQALSLVQKTGQKYKVPTTSDIHAHDEA
                     APAADFVDVLQIPAFLCRQTDLLVAAAETGKVINVKKGQFVSGPAMKFAAEKIIRAGN
                     EKIIFTERGTTFGYQDLVVDYRNIPWMQDLGFPVVMDCTHSLQQPNQTAGVTGGNPQL
                     IGTIAKAAIAAGAEGLFIETHPNPAIAKSDGANMLKLDLLEELLGKLVKLRKAVV"
     gene            1014..1451
                     /locus_tag="VGM24_01625"
     CDS             1014..1451
                     /locus_tag="VGM24_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154087.1"
                     /translation="MKFALLALITVLSFSACKKTTVVNETVDQAYSAIYTIDPADWVQ
                     GQDPGSGNFFYSSTLTIPELDDIINTHGGVQVYISFDDLNAATKTYESLPEVFDGVAY
                     GTLHSTGSVTIDLRDAGGGSLTSPITQSITAKVVLIDAQPLDN"
     gene            complement(1464..3053)
                     /locus_tag="VGM24_01630"
     CDS             complement(1464..3053)
                     /locus_tag="VGM24_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY4154088.1"
                     /translation="MGYFAFTDHSTLAMPKKIQTDSGIEIKPFYQATDAAPMLEKAGE
                     FPYTRGIYPDMYRSKRWTMRQYAGFSTAAESNKRYHYLLNQGVSGLSVAFDLPTQIGY
                     DSDHPIAEGEVGKVGVAIDSIQDMEELFAGIQLEQVSTSMTINATGFILLALYVALAK
                     KQGADLKKISGTVQNDILKEYAARGTYIYPPAPSMRIITDTFEWCSRELPKWNSISIS
                     GYHIREAGSTAVQELGFTLSNGKAYVKAALEKGLDINVFGKRLSFFFNAHNNLFEEAA
                     KFRAARRMWAQIMKEQGATDPQALMLRFHAQTGGSTLTAQQPLNNISRVTIQALSAVL
                     GGAQSLHTNGYDEALGLPTEEAAGIALRSQQIIGYESGAADTADPLGGSYFVESLTSS
                     IEQAAWELIKKVDELGGSVAAIEEGYIQNEIANSAYLHQREVEAKEKIIVGVNAFQSP
                     EKEPAPVFRVDDSIRQVQTEKLARLRAQRDHAKCDQLLQLLNDKASSGENIMPVVIEA
                     VEAKCTLGEIADTLREIFGEY"
     gene            3138..3668
                     /locus_tag="VGM24_01635"
     CDS             3138..3668
                     /locus_tag="VGM24_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154089.1"
                     /translation="MTLSNTELIIAIIFAVSIAGALAFLFWKQRKEVKRGTIHPAQTE
                     NVSVTRTLKLQAYERLILLADRIAIPNLVSRTNQPGLSAREMQSLLTQVLRQEFDHNI
                     TQQIYVSPEAWDAVRNLKEQNLLIVNQVASFLPAEASGQDLNKSLLELVMQNPKVSLH
                     NVVADALAFEAKKMMD"
     gene            3725..4069
                     /locus_tag="VGM24_01640"
     CDS             3725..4069
                     /locus_tag="VGM24_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY4154090.1"
                     /translation="METIIQSPLHFTPQAVQEIRRLMNEEGFDRNAFLRIGVKGGGCS
                     GLSYILGFDKMQENDEKYESEGLSFIMNPAHGIYLGGMEIHWENGLNARGFTFNNPNA
                     SKTCGCGTSFAV"
     gene            complement(4089..>4635)
                     /locus_tag="VGM24_01645"
     CDS             complement(4089..>4635)
                     /locus_tag="VGM24_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4154091.1"
                     /translation="SKRLLEVVPISLLLIFLLLFIMFGNLKDAGLVFLNVPFAVVGGI
                     LALLITGTNFSISAGIGFIALFGICILEGVLLITAFKQNLEKLRGENPLFTSVKRGVN
                     SLIRPVLMTALMATIGLLPAALSTGIGSESSRPLARVVIGGLICATIFSLLIFPVVFF
                     LSYRNVDTRHQGENEDRIETT"
BASE COUNT         1099 a         1166 c         1180 g         1190 t
ORIGIN      
        1 ctcgctttct ggctgaacat taaacgatat atcggtttgg ccaaaaggaa tccgctgaat
       61 agttaaggct ttctccggcc tggcttatac cacggccttt ctgagcttca ccagtttccc
      121 cagcaattct tccagcagat ccagtttgag catattggcg ccgtcgcttt tcgcaatggc
      181 gggattggga tgggtttcaa tgaacaggcc ttccgctccg gcagcaatgg ctgctttggc
      241 aatagtgccg atgagttggg gattgcctcc ggtaacgccg gcggtctgat tgggctgttg
      301 gagggaatgg gtgcaatcca tcacaacagg aaatccgaga tcctgcatcc agggtatatt
      361 ccggtagtca accacgaggt cctgatagcc gaaggtggtt cctctttccg taaaaatgat
      421 tttttcattt cctgcccgga taattttctc tgcggcaaat ttcatggcgg gtccgcttac
      481 aaactggcct ttcttaacgt tgattacttt ccccgtttcc gcggcggcca ccaggagatc
      541 ggtctgccgg cacaagaatg ccggaatttg caaaacgtcc acgaaatccg ccgccggggc
      601 ggcttcatca tgggcatgaa tatccgaggt ggtgggaacc ttatattttt ggccggtttt
      661 ttgaactaac gacagagcct gctcatcacc aattccggca aaggaagagg agctggtccg
      721 gttggcttta cgataggacg ctttaaagat ataggggata cccaggtttg cgcaaagaac
      781 ggaaactttt gaagccaccg tgtccagtaa ttcttcgttt tccaccacgc agggacctgc
      841 gatgagaaag aagttctttt catcgtattg ctggccccgg aagagttctt gcaaaaaggc
      901 tttcatgaaa aaaagttaaa taaatccagg gcgaaagtaa tttgattttt ttttacggga
      961 ttaaactatt ttcatgcccg ggagtctgag gatcgtacaa caaaaacaat catatgaagt
     1021 ttgctctatt agccctaatt accgttctat cattttctgc ctgtaaaaag acgacggtcg
     1081 taaacgagac ggttgatcag gcctattcag ccatttacac gattgatccg gctgactggg
     1141 tccaggggca ggatccgggc agcgggaatt ttttctattc ttcaacgctt actattccgg
     1201 aactggacga tatcatcaat acgcatggcg gggtacaggt atatatttct ttcgatgacc
     1261 tgaatgcagc tacaaaaacc tatgaaagct tgccggaagt ttttgacgga gtggcttatg
     1321 gaaccctgca ttcgaccgga tcggtaacca ttgatttaag ggatgccggc ggtggcagtc
     1381 tgacaagccc cattacccag tctattaccg caaaggtcgt actgatcgat gcgcagccgc
     1441 tggataatta aattctgccc ggatcaatat tcaccgaata tttcccggag ggtatcggca
     1501 atttccccca gcgtgcattt tgcctcaacg gcttcgatta cgacgggcat aatattttct
     1561 cctgacgaag ctttgtcgtt caggagttgc aataactggt cacatttcgc gtgatctctc
     1621 tgcgcgcgaa gtctggccag tttttctgtc tgaacctgcc ggatcgaatc atcgacccgg
     1681 aataccgggg caggttcttt ttcgggggac tgaaatgcat tcactccaac gattattttt
     1741 tctttggcct ccacttcccg ctgatgcagg taggcgctgt tcgcaatttc attctggata
     1801 tatccttctt caatggcagc cacgctacct ccgagttcgt ccactttttt tataagctcc
     1861 caggcagcct gttcaatgga tgaggtgagg ctttcaacaa aataactgcc gccgagcgga
     1921 tctgccgtat ctgccgcacc gctttcatat ccgatgatct gctggctgcg cagggcgatg
     1981 cccgccgctt cttcggtagg taaacccagt gcttcgtcat acccgttggt gtggagtgac
     2041 tgggctccgc caagcaccgc ggaaagggcc tgaatggtca cccgggaaat attattcagc
     2101 ggctgttggg ccgtcagggt gcttccgccg gtttgcgcat ggaaccggag catcagggcc
     2161 tgcgggtccg tcgctccctg ttctttcatg atctgggccc acattcggcg ggcggcgcgg
     2221 aatttggccg cttcttcaaa cagattattg tgggcattga aaaaaaaaga gagtcttttg
     2281 ccaaaaacat taatatccag tcctttctcc agtgcagctt tcacataggc tttcccgtta
     2341 gaaagagtaa acccaagttc ctgcacggcg gtactgccgg cttcccggat atgataaccg
     2401 gaaatcgaaa tgctgttcca tttgggaagt tccctgctgc accattcaaa ggtgtccgtg
     2461 ataatccgca tggaaggcgc cggcggatag atataggttc cccgggcggc atattctttt
     2521 aatatatcat tctgtaccgt acccgaaatt tttttgagat ccgctccctg tttttttgcc
     2581 agcgctacat acagggccag caggatgaag ccggtggcat tgatggtcat ggaagtactc
     2641 acctgctcga gctggattcc ggcaaacagt tcttccatat cctgaatgga atcaatggcg
     2701 acccctactt ttcccacctc tccttcggcg atcggatgat cgctgtcgta cccgatctgg
     2761 gtgggcaggt cgaaagccac actcaacccc gataccccct gattcaacag gtaatgatag
     2821 cgtttgttgc tttccgccgc tgtgctgaag ccggcatact ggcgcatggt ccaccttttc
     2881 gagcggtaca tgtccgggta gattccgcgg gtatacggga attcccctgc cttttccagc
     2941 atgggggcgg cgtctgttgc ctggtaaaac ggttttattt ctattccgga atcggtttga
     3001 atttttttcg gcatagccag ggttgaatga tcggtaaagg caaagtaccc cattttttga
     3061 ttctgccgct tacaattatt tcatcctcat ggaaatattg catttcgtat tttcgtttac
     3121 tcttaaaata aaagccaatg accctcagca atacggaact gatcatcgcc attatttttg
     3181 ccgtttctat tgcgggagca ctggcttttc tcttctggaa gcaacgaaaa gaggtgaaac
     3241 gcgggaccat ccatccggcc caaacggaaa atgtatcggt cacccggacg ctgaaactgc
     3301 aggcttacga aaggctgatc ctgctggccg accggatcgc cattccgaac ctggtcagca
     3361 gaaccaatca acccggactc agtgccaggg aaatgcagtc gttgctgacg caggtgcttc
     3421 gccaggagtt tgatcacaat atcacccagc agatttatgt cagtcccgaa gcctgggatg
     3481 cggtccggaa cctcaaagaa caaaacctgc ttatcgtgaa tcaggtggcc tcttttcttc
     3541 cggccgaggc cagcggtcag gacctcaaca aaagcctgct ggagctggtt atgcagaatc
     3601 cgaaggtctc cctgcacaat gtagtggcgg atgccctggc ttttgaggct aagaaaatga
     3661 tggattagaa agctgcgggg aagagccgga ataagattaa atttgcagat taattttgac
     3721 aggtatggaa accattattc agtcgcccct tcactttacg ccgcaggccg ttcaggagat
     3781 ccgccgcctg atgaatgaag aggggtttga cagaaatgct tttctccgga tcggggtgaa
     3841 gggtggcgga tgctccggcc tcagctatat cctgggattt gataaaatgc aggagaacga
     3901 tgaaaaatac gaatccgaag gcctttcctt tatcatgaat ccggcgcacg gcatttacct
     3961 gggcgggatg gagatccact gggaaaacgg cctcaatgcc cgcggcttca ccttcaataa
     4021 cccgaatgca tccaagacct gcggatgcgg aacttcgttt gcggtttgat tcccggtcat
     4081 ccgctttttc aggtggtttc aatcctgtct tcattctccc cctggtgccg ggtatccaca
     4141 ttccggtaag aaagaaagaa taccaccggg aaaataagta aagagaaaat ggtagcgcag
     4201 atcagtccgc cgatcaccac gcgggcaagt ggtcttgaac tctccgagcc gatgccggtg
     4261 gaaagggcgg ccggcagcaa tccgatggtt gccatcagcg ccgtcatcag aacgggccgg
     4321 atcagggaat tcacaccgcg cttcacggag gtgaacaaag gattttcgcc ccgcagtttt
     4381 tccaggtttt gtttaaaggc ggtgatcagc agaactcctt ccaggataca gataccgaac
     4441 agggcgataa atccgatacc tgccgaaatg ctgaaattcg ttccggttat caacagggcc
     4501 agtatgccgc cgacaaccgc aaacggcaca ttcaggaata ccagtccggc atccttcaga
     4561 tttccaaaca tgatgaaaag caggaggaag atgaggagaa gactgatggg tactacttcc
     4621 agcaatcgtt tggat
//
LOCUS       DASYCU010000083         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_168035, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000083 DASYCU010000000
VERSION     DASYCU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168035"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VGM24_01650"
     CDS             <1..1386
                     /locus_tag="VGM24_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 39"
                     /protein_id="HEY4154092.1"
                     /translation="PSAVSVHWDKPILVSKTTPTLQVVVNPPLRPGQPLSAAAYQALK
                     DLGADYVRFVPWLPYPRLAVAELKPPAGGQTFWDFSLLDSTMKAFMEATQGHSVVINF
                     STIPAWMWKTESPVRYPDDPYQPSWDYNQGTQLRDSSMKEVAAYYARVFSWYAKGGFT
                     DELGKFHQSGLYYKIPYWEVLNEPDLEHNIPPQVYTKIYDAVVGELKKISPSTKFVGI
                     TVAFNNDPEWFEYFLNPKNHKPGIPLDAISYHHYSTPSDAKQGVDQWQYTFFDKADDF
                     LHRVRYIESIRKRLSPKTITTINEIGTIINDSPTREYYSLSGALYAYIFLELTKMGID
                     VAGESQLVGFPSMFPDVSMTDWETGNPNARFQVLKLIKDNLGPGDQLVETVSGSGIET
                     QAFITAKGKKLLLINKRNREVQVKLPEDLKGAVADYVDESTNGLPSSREALMSNVILL
                     KPFAVAVVRLK"
     gene            1497..1580
                     /locus_tag="VGM24_01655"
     tRNA            1497..1580
                     /locus_tag="VGM24_01655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1531..1533,aa:Leu,seq:cag)
     gene            1749..>2518
                     /locus_tag="VGM24_01660"
     CDS             1749..>2518
                     /locus_tag="VGM24_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY4154093.1"
                     /translation="MTIEDRLSANGKKTYYRFVWGRKSSDKMSAGVFTYSKPKTQTEK
                     NHNKEALAILEIKRSRLVLDRQAIGTGYIPAHRYKHNFVDFFEDFVKKNIRKGKRHLH
                     ACFEHFKTFIGKRSLPPVEITEEFCQRFRNYLLDKFNGDTPMNYFSEFKRMIKAATKQ
                     GYFRNNPAEDIKAKAGKNRRLKEHLEVEEYLQLLKTPCLNEEVREAFIFCCYTGLRWV
                     DVKQINWLDLEKDTIKTRLIQAKTGQAVVITAHPIAKA"
BASE COUNT          684 a          594 c          621 g          619 t
ORIGIN      
        1 ccatcggcgg tatccgttca ttgggataag ccgatactgg tctcgaagac cacgcccact
       61 ttgcaagtgg tcgtgaatcc accgctgcgg ccggggcagc cgttgagcgc agcggcttac
      121 caggcgctca aggacctggg cgcggactat gtacgctttg tgccgtggct tccctatccg
      181 cggctcgcgg tggccgagct gaagccgccg gcggggggac agaccttctg ggatttcagc
      241 cttctggaca gcacgatgaa agcctttatg gaagccacgc agggtcattc cgtcgtgatc
      301 aatttcagca ccatacctgc ctggatgtgg aaaacggaat cgccggtccg ctacccggat
      361 gatccttacc agccttcctg ggattataat cagggaaccc agctgaggga cagcagcatg
      421 aaagaagtcg ccgcctatta tgcgcgggta ttcagctggt atgccaaagg tggatttacg
      481 gatgagctgg gcaaattcca ccaatccggt ttatactata agataccata ttgggaagtg
      541 cttaatgagc cggacctcga acacaatatc ccgccgcagg tctatactaa gatctacgat
      601 gccgtcgtcg gcgaattaaa aaagatatct ccgtccacaa aatttgtcgg gatcacggtt
      661 gccttcaaca atgacccgga atggtttgaa tattttttaa atccgaaaaa tcacaaaccg
      721 ggaattccgc tggatgcgat ttcctaccat cattattcca cgccttcgga cgcgaagcag
      781 ggggtagatc aatggcagta tacttttttc gacaaggcgg atgatttcct gcaccgggtg
      841 cggtatatcg aaagcatcag aaaacggctt tctccgaaaa ccattacgac catcaatgag
      901 atcgggacga tcatcaatga cagtccgacc agggaatatt acagtctttc gggcgccctc
      961 tatgcctata tctttcttga attgacaaaa atgggaattg atgtggccgg agaatcccag
     1021 ctggtcggat tcccatccat gttcccggat gtaagcatga cggactggga aacggggaat
     1081 cccaatgcgc ggttccaggt attaaagctc attaaggaca acctgggccc cggagatcaa
     1141 ctcgtggaaa cggtatcggg atccgggatc gaaacccagg catttataac ggcaaagggg
     1201 aaaaagctcc tgctgatcaa taagcgcaac cgggaagttc aggtaaagct gccggaggac
     1261 ctgaagggcg ctgtggccga ttatgtggat gaatccacca atgggctgcc ctcttcccgc
     1321 gaggccctga tgagcaacgt gattctatta aagccgtttg cggttgcggt ggtccggttg
     1381 aaataaaggg tttgctgaaa gttctatccg ttgttttcct ttcccggaac caggaatgac
     1441 aaaaacaacc gcctgtaatt ttttgttttc gatgaatcct tctacatttg ctgcccgccc
     1501 gggtggcgaa attggtagac gcactgtgtt caggtcgcag cgtgggaaac catgtgctgg
     1561 ttcgaatcca gtcccgggca cggaaaaccc tcattaaatc attgattttc agagggtttt
     1621 gtatttttaa catgcttttt ttctccactt ttctacgctt atccacactg gtttgcaccc
     1681 ataaatcacc cacacttgtt cccacacttt tggaacttag tttgtggcat agtcataaag
     1741 cccaatttat gacaattgaa gaccgactct ctgccaacgg aaagaagact tattaccgat
     1801 ttgtttgggg aaggaagtct tccgataaaa tgagcgcggg tgttttcacc tatagcaaac
     1861 ccaaaaccca gaccgaaaaa aatcacaata aggaagcact tgctatcctt gaaataaaaa
     1921 ggtctcggct ggtactagac cgtcaggcca ttggaacggg atatatacct gcccaccggt
     1981 acaagcataa ctttgttgat ttttttgagg atttcgttaa aaaaaatatt cgcaagggca
     2041 aaaggcattt acacgcctgt tttgaacatt ttaaaacctt cattggaaaa agaagcctgc
     2101 cgcctgtaga aatcaccgaa gaattttgcc aacgcttccg caattattta cttgacaaat
     2161 ttaatgggga taccccgatg aactattttt cggaattcaa acggatgatt aaggcggcga
     2221 caaaacaggg ctattttcgc aataatcctg cagaggatat aaaagcgaag gctgggaaaa
     2281 accgaagatt gaaggaacac ctggaggtag aggaatacct gcaacttttg aaaacacctt
     2341 gtttgaatga agaagtccgg gaagcattta ttttttgctg ctataccggt cttcgctggg
     2401 ttgatgtgaa acagattaat tggttagatc tggaaaaaga cactatcaag acccggctga
     2461 tccaggcaaa aactgggcag gctgtcgtaa ttactgcgca cccgatcgca aaagccat
//
LOCUS       DASYCU010000084         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_312042, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000084 DASYCU010000000
VERSION     DASYCU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312042"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..2518)
                     /locus_tag="VGM24_01665"
     CDS             complement(134..2518)
                     /locus_tag="VGM24_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154094.1"
                     /translation="MIKNLLLVAIRNLKKDKWYAVINITGLMIGITFSLFLIFYITDE
                     LSYDRYNSNAARIYRVVSFIKEPDRDLSHNASTQFPLVPEIRKDFPEAEEAVRLAGKD
                     RTLLVNGNLKIYEDKIFFSDSNLFNVFTYSFIEGNPNNALVAPNSMVLTQSTAKKYFG
                     KDRTVVGKTLTNDKGEIFRITGVIKDVPKNSHIIFNALISMSSLGQGYGNNWGGFGTY
                     SYVLLRPSSNVAAFEKKMLTLYDNFMAPIFAQYNIKIHYGLQPITAIHLHSDMANEPE
                     QLGSISYIYIFSVVAAFMLLIACINYMNLTTARSAGRAKEIGIRKVTGSTRTNLVLQF
                     LIESVLTTIIAFAFSLVLFGLFLPTFNLLSGKSLELSVLVRPANLLMLLGIALFVGLL
                     GGSYPAFYLSKFNPVSMLKGSLSKSSSNATLRRALVIFQFSVSMAMLTCTWIVFKQLQ
                     YLRQKDLGFNKEQVLTLSADASTDIRSNILAFTNEIKKNPDIRSVSTSQSVPGGLNSF
                     YLFSIETKDGFTQKGVGNYAVDENYFNTLGIKIIKGRNFRGLSDTLRSIIVNEAMAKQ
                     YGWKDPIGKKVKFPEDSSSSYFEVVGVVKDFNQSALYNPIAPLILFYQPNNNRIQVKL
                     EAKNIPAAIASLEKTWKTDFTDIPFSYTFLDRDFDSQYNADQRRGKIFMAFSILTVMI
                     TCLGLLGLIAFITMQRQKEISIRKVMGARVGQIVPLLTGNFIWLVIISSLIAFPVAAI
                     FMAKWLKLFFYNTGLTVQPFLFSALIVLLITVITVIFHTVKAATASPVKGLRSE"
     gene            2764..>3456
                     /locus_tag="VGM24_01670"
     CDS             2764..>3456
                     /locus_tag="VGM24_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY4154095.1"
                     /translation="MKSHSAAGSGQFINFNGALLDSATPVISASNRSFRYGDGLFETM
                     RWENGEIRLGAFHFDRFFGGLKILQMECPADFTSGFLSAEIAELCRKNKRQRARVRLN
                     VYREESAALYPENNRPRFVIECSDLPEAGLKSLRATIYSAEKKPAGILSNLKTNNYLL
                     NVMAVKFARQNGFDDALILNSGDRLCEAGSSNLFFIKERSIYTPSLAEGCVAGVMRRH
                     LLNHLPQLGFSVH"
BASE COUNT          913 a          793 c          830 g          920 t
ORIGIN      
        1 gcaggggtgg ccaacccggt aaagagcctg aggacggaat gatcaaaagt ctccgcaact
       61 tttaaatgaa tgaactgatc gaacaagcct actttctgcc acggcaacaa taagttggat
      121 gattactaag ttcctattcg cttctcaatc cttttaccgg acttgccgtt gctgctttaa
      181 ccgtatgaaa gatgaccgta attacggtga tcaacagtac gataagcgct gaaaacaaaa
      241 acggttgaac ggttaagccg gtgttgtaaa agaagagttt cagccatttg gccataaata
      301 ttgctgcgac cggaaaagca atcagggagg agataatgac cagccagata aaattccccg
      361 tcagcagggg aacgatctgc ccgacgcgcg ctcccattac tttcctgatg ctgatttcct
      421 tttgccgttg catggtaata aaagcgataa ggcccagcag tcccaggcag gtgatcatca
      481 ccgtcaatat tgaaaaggcc atgaatattt tacctcttct ttgatctgca ttgtattgtg
      541 aatcaaaatc ccggtcaaga aaggtgtaag aaaagggaat gtccgtaaaa tccgtcttcc
      601 aggtcttttc gagggaagca atggcggcgg gaatattttt tgcttcgagt tttacttgta
      661 tccgattatt gtttggctgg taaaacagaa tcagcggcgc aatgggattg tacaaggcgg
      721 actggttaaa atctttaacc acgcccacta cttcaaagta agaggaggag ctatcttccg
      781 gaaattttac tttttttccg atgggatcct tccagccata ttgcttagcc atggcctcat
      841 tgacaatgat gctcctgagg gtgtcggata gccccctgaa attccttccc tttattattt
      901 ttatcccgag ggtgttgaaa taattctcat ccacggcgta attcccaact cccttttgag
      961 tgaatccgtc ttttgtttcg atcgaaaaca ggtagaaact gttgagtcct cccggaacgc
     1021 tctgcgacgt gctcaccgaa cggatatcgg gattcttttt aatttcattg gtaaaggcca
     1081 gaatattgct gcgaatatcg gtgcttgcat cggcggataa ggtcaacacc tgctccttgt
     1141 tgaacccgag gtccttttgg cgcaggtatt gcaattgctt aaatacgatc cacgtgcagg
     1201 tgagcatggc catggaaacg gagaactgga aaatcaccag cgcgcgacgc agggtggcat
     1261 tgctggagct tttggaaaga cttcccttca gcatgctgac cgggttgaat tttgaaagat
     1321 aaaaggcagg ataacttccg ccaagcagac cgacaaaaag cgcgataccc aaaagcatca
     1381 gcaggttggc aggccggacc agcacggaga gttccaatga tttccccgaa agcaggttaa
     1441 atgtggggag aaatagtccg aataatacca ggctgaaagc aaacgcaatg atcgtggtca
     1501 ataccgattc gatcaggaac tgcaggacca ggttcgtcct tgttgaaccc gttactttcc
     1561 ggataccgat ctcctttgct cttcccgccg aacgcgcggt ggttaaattc atgtaattga
     1621 tgcaggcaat gagcaacatg aaggcagcga cgacggaaaa aatatagata taagaaatgc
     1681 tgccgagttg ttccggttca ttggccatat cggaatgcaa atggatcgcc gtaatgggct
     1741 gcaggccata atgtattttg atattatatt gtgcaaaaat gggcgccatg aaattatcat
     1801 aaagcgtcag catctttttt tcaaacgcgg ccacattgct gctgggtctc aaaagaacgt
     1861 agctatacgt gccaaaacct ccccagttgt ttccatagcc ctggccaagc gaactcatgg
     1921 agatcagggc attaaagatt atatgcgagt tttttggaac gtcttttatc acgccggtga
     1981 tccggaatat ctctcccttg tcgttcgtca gcgtttttcc cacgacagtc ctgtcttttc
     2041 caaaatattt tttggcagtt gactgtgtca gcaccatcga gttgggggca accagcgcat
     2101 tattggggtt cccttctata aaggaatagg taaatacgtt gaatagattg ctgtcactaa
     2161 aaaatatctt gtcctcgtaa atcttcagat tgccgttcac caataaggtt ctgtcctttc
     2221 cggcgagacg gaccgcctcc tctgcttcgg gaaaatcctt ccggatctcg ggtaccaggg
     2281 ggaactgggt ggaagcgtta tgagacaaat cccggtccgg ctccttaata aacgatacca
     2341 cgcgatagat ccttgcggca ttgctgttgt accggtcata acttaattca tccgtaatat
     2401 aaaaaatcag gaaaagactg aacgtgatgc caatcataag ccccgtaata ttgattaccg
     2461 cataccattt atcttttttc agattgcgga tggcgacgag cagaagattt tttatcatgg
     2521 acttattttt attctgacct cagactttgc gtccgacgca ccggtttaaa ccggtacggg
     2581 ttgttcggtg aaatttcatg ccagaaagca agagattaaa tatcaatcca ttgaaacctg
     2641 agaggaagaa aaagctgatc gtttttgata catcttgttt gtttttgatg cacagcatca
     2701 tccctcattc tctacgcacc gggcggagcg aaaaatccta attaaggtta acttcgccgc
     2761 ttcatgaaat cccattccgc ggccggttca ggccagttca tcaatttcaa cggggcactg
     2821 ctggattccg caacgcccgt gatctccgct tccaaccgga gtttccggta tggagacggg
     2881 ttatttgaaa ccatgcgctg ggaaaatggg gagatccgcc tcggggcttt tcatttcgat
     2941 cgcttcttcg ggggattgaa gattttgcag atggaatgcc cggcggattt tacctccgga
     3001 tttctttccg cagagatcgc tgagctctgc agaaaaaata agcggcagcg cgcgcgcgta
     3061 cgactcaatg tttaccggga agaaagtgcg gcattatatc cggagaataa ccgtccccgg
     3121 ttcgttatcg aatgttcgga tcttccggag gccggactga aatcccttcg ggcaacaatt
     3181 tattccgcag aaaaaaaacc ggcgggcatt ttaagcaatc tcaagaccaa taattacctg
     3241 ctcaacgtga tggcggttaa attcgccagg caaaacgggt ttgacgatgc cctgatcctg
     3301 aattccgggg accgtctctg tgaagccggt tcgagtaatc ttttttttat taaagaaagg
     3361 agcatttata cgccgtccct ggcagagggc tgtgtcgcgg gcgtgatgcg aaggcatctt
     3421 ctgaaccatt taccgcagct gggtttttcc gtgcat
//
LOCUS       DASYCU010000085        10138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3502188, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000085 DASYCU010000000
VERSION     DASYCU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502188"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGM24_01675"
     CDS             complement(<1..188)
                     /locus_tag="VGM24_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4154096.1"
                     /translation="MDAKGKKILIADDEPDILEVLQYNLAREGYEVSTAKDGDDALYK
                     AKMTHPDLIILDIMMPKK"
     gene            365..2584
                     /locus_tag="VGM24_01680"
     CDS             365..2584
                     /locus_tag="VGM24_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154097.1"
                     /translation="MKKFTRFVIFPILLFPALAVLGQMARPPFKIQQERKLWHENIDR
                     EQQRLLALDGKADTIIQVSKDENVNLEIADVMIRQVDELQEKIELDSVLTAQMKIKNL
                     RSLESMLKGYTSHYRQKDFPVSMAQALFDAFVRAMELDRRNKSIEPVIAENSYGIGKI
                     LVECFLLPVENVGVRDSRNILIRKYCELHPDQIFTILSNNPSLPFADSLIILGGKHNI
                     RKLYDYASGNSALSSRIRNSPDSLIRTVAKIAASRSGQLYLPFLDNLLHRKITVDEIN
                     KVKDDNLNYYRLLVKTRLDYVERMLPPQHDTAVEMESLTRMLESKGKDVFIREINALH
                     DKPASVRFKILDKLTPAELYYLVVLSEDEIYTSSYLGVYDRIFQRMNVPRGDSLIMQM
                     HGDYFRKFIKMAAAYNKLDHFLATMDKYNAATIMKAFIIGLERTNEEDAVDVADSYSS
                     IFDKNPSLAAFVLNEVKWNYKRNLLNDDKKGTIIYHLLEALFESADTSNHTDLSARLG
                     IPPVYSIDYDSLKDDSGRVIQQVFFYGDEDKDGQHSYDDYLRLFLHKPEWKITMNPTK
                     EWVTIQSVKGKPVIIFANRPLYGEDDPDDKAQHHLDDYLYDNKLKPTIYIHRGHSYHV
                     RYTLQQIQPSAKIVVLGSCGGYNNLSEVLSISSDAHIISSKQVGTKSVNEPILQAINN
                     NLLEGNNIDWLPIWKGLTEKFRKDPVARERFDDYIPPYKNLGVIFIKAYRKAMGEED"
     gene            2612..4423
                     /locus_tag="VGM24_01685"
     CDS             2612..4423
                     /locus_tag="VGM24_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4154098.1"
                     /translation="MADSVSRKKIFDFSLLQRVLRYAAPYKKWFYGSIALSILLAVIS
                     PLRPFLIQYTVNHYIRGGITGDPSLRERMISLIVYISVVQIGLLLIETVFRFFFSFLT
                     SWLGQTVVKDLRIAVYKKILGLNLSQYDKTPIGTLTTRTINDIEAINDIFSEGFIPIV
                     SDLLSIVSILLFMFLANWQLTLVCLIPFPFLILSTYFFKEAVNKSFIRVRNAVAALNA
                     FVQEHITGMQIVQAFAAEDQEYKSFRKINSEHRNANIRAIFAYSVFFPIVEIVLAVST
                     GLLVWWGAELVLDLPPAQAARLTGEIIAFFLYLNLLFRPLRLIADKFNTLQMGMVASE
                     RVFRVLDNEDHIRSEGGYAPARLLGKIEFDRVYFAYTDERYVLKNISFLVQPSETLAI
                     VGHTGSGKTSIISILNRLYSIQKGQIRLDDRAIEMYDLDALRSSIGVVLQDVFLFSGS
                     ILENITLRNPDIPFEKVVQAAKLIGMHDFILELPGQYNYNVMERGSTLSLGQRQLLSF
                     IRALLFDPSILILDEATSSVDTGSERLIQEAIGKLIADRTSIVIAHRLSTIRKASKIL
                     VLDKGEIKETGTHEELMEIQGFYYRLHQMQFEEKVDS"
     gene            4544..5665
                     /gene="atpB"
                     /locus_tag="VGM24_01690"
     CDS             4544..5665
                     /gene="atpB"
                     /locus_tag="VGM24_01690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY4154099.1"
                     /translation="MMQGRFKSLLVAGFSVFLLLFSGVLRAQENQAEKGGKKHQTFDA
                     NEVIFNHVLDAHEFHFLSYRSLEGREHQVSIPLPVILYSPQRGFSSFLSSRFHHGEEA
                     YQGYKLAGNKIVPVNADGSVDPSVKVYDISLTRNVVQMMIALALLVFLLVRIAGKYKS
                     GTGTTRAPTGFQNLMEPVITFVRDEVAVPNLGTKYQKYLPYLLTVFFFILINTMVGLV
                     PGSANVTGNIAFTLVLGMISFIVITVSGNKHYWGHIINPPVPFGIKFIMIPVEILGIF
                     TKPFALIIRLFANMVAGHIIIICLVSLIFIFGELNKGIGWGFSPVSIGFAVFIYLIEI
                     LVAFIQAFIFANLTAVFIGQAIENNETHSGNGHDDAVIV"
     gene            5704..5952
                     /gene="atpE"
                     /locus_tag="VGM24_01695"
     CDS             5704..5952
                     /gene="atpE"
                     /locus_tag="VGM24_01695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY4154100.1"
                     /translation="MDLGTLLLSTTPWANAGGAIGAGLAAIGAGIGIGQIGKGAVEAI
                     ARQPEASNDIRANMILAAALVEGVALFGVIAGILAMFV"
     gene            6035..6529
                     /gene="atpF"
                     /locus_tag="VGM24_01700"
     CDS             6035..6529
                     /gene="atpF"
                     /locus_tag="VGM24_01700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY4154101.1"
                     /translation="MDLLIPSFGLIFWTLLAFVIVVMVLKKFAWKPILQSLNDREKTI
                     SDSLATAERVKAEMAQMKSENEALLARAREERALMLKEARDTKDKMISEARDQAKTEA
                     GKILSDAQVAIEQQKMAAITDLKNQMGNLVIEVSEKILRRQLENKSEQEKYIRQLTSE
                     VKLN"
     gene            6588..7151
                     /gene="atpH"
                     /locus_tag="VGM24_01705"
     CDS             6588..7151
                     /gene="atpH"
                     /locus_tag="VGM24_01705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY4154102.1"
                     /translation="MLNPRIAGRYAKSLIDLAVEKDQLEAVYQDMVYLQNLNQASPEF
                     VRILKSPVIKGDKKEKILEALTKDKMGNISQSFNRLLVKKGREAYIPEIVSAFIQQYK
                     DYKGIHIVSLTTAVPLTEESRSAIVNRINESAQLKEVELRTKVDPDIIGGFIIEVDGR
                     LMDASILYDLNTIKKQFESNDFIYKIR"
     gene            7182..8759
                     /gene="atpA"
                     /locus_tag="VGM24_01710"
     CDS             7182..8759
                     /gene="atpA"
                     /locus_tag="VGM24_01710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY4154103.1"
                     /translation="MAEIKPDEISAILRQQLSNFNVSADMEEVGTVLQVGDGIARVYG
                     LNNVSSGELVEFENGVRAIALNLEEDNVGVVLMGESGDIKEGAKVRRTGKIASIQVGE
                     GMLGRVVNTLGLPIDGRGPVQGEKHEMPLERKAPGVIFREPVKEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQKEFHKAGKPVICIYVAIGQKASTVAGIMKT
                     LTDHGAMEYTVIVAASASDPAPLQFYAPFAGAAIGEFFRDTGRPALIIYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIISDDAVAKNMNDLPESLKSQVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLEGNLFNSGIRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQALYREMEAFSKFGGDLDASTKLVLDKGARNVEILKQPQY
                     SPVAVEKQVAIIYLGTQGLLRNVPVKRIKAFEEHFLMEMENKLPEVLAEFKKGGLSDD
                     AIRRTTALANELIPQYK"
     gene            9113..>10138
                     /locus_tag="VGM24_01715"
     CDS             9113..>10138
                     /locus_tag="VGM24_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154104.1"
                     /translation="MKQIHLRRLKGESGRMIRNGLTVLFFLSQYTGLHAQSLVPELVF
                     MNPQLKTGSGCPDAGKDGAVYIFSNVGVGIDALVSIQGRSSSKVNLSAIDLKGPEQDP
                     AKGTGYDNAWQPKVSYGNGVATAHSNWWMEFKISFVKHDNPDQSVSVNQFFVSGLDID
                     GDGSRLHEFQSYYKMQYFTLEKNTDMASSSVSGCLNDPQLEGKRFDGPVKNYEGITTS
                     ASEVMVSNFYNNASSLVVRVGAETGSAGSQATDRMYALWFKSLTYDVPVSAPLPLTHI
                     AFNAQLRNNKDVHLNWTTSMEQNISHFTLQRSVNGKDFDDAAVLFTEGNSAGKKNYQL
                     TDHLPPVS"
BASE COUNT         2679 a         2408 c         2497 g         2554 t
ORIGIN      
        1 ctctttttcg gcatcatgat atccagaata atcaggtcgg gatgggtcat ttttgcttta
       61 tacagggcat cgtccccgtc ctttgccgtt gaaacttcat atccctcccg ggccagattg
      121 tactggagga cctccaggat gtccggctca tcatcagcga tcaggatttt cttgcctttg
      181 gcatccatta acatataatt tacgcaaacc taatagaaat atagcgttag gcaggagaaa
      241 cagtccgagt ttacaatttt ttaatacgga gacggcggat gccctgtttg accagccttt
      301 tcgcggggct tgcgcttacc ggaaatggct gtatttttgc ctcgctctgt tcagtcgcct
      361 atatatgaaa aaattcaccc ggttcgtcat tttcccgatc cttctctttc ctgccctggc
      421 ggttttgggc caaatggcca ggcccccctt taaaatacag caggagcgaa agctttggca
      481 cgagaatatc gaccgggagc agcagcgcct cctggctctg gacggcaagg cagacacgat
      541 tatccaggtt tcaaaagatg aaaacgtgaa tctggagatc gccgatgtca tgatccgcca
      601 ggtggatgaa ctccaggaaa agatcgaact ggattccgtg ctgaccgccc agatgaaaat
      661 caagaacctg cgttccctgg aatccatgct gaaaggctat accagtcatt accggcagaa
      721 ggattttccg gtttcaatgg cccaggccct ctttgatgcc tttgtccggg cgatggaact
      781 ggaccgccgg aataaaagca ttgaacccgt tatcgcagaa aacagttacg gcatcggcaa
      841 aatcctggtg gaatgctttc tgttaccggt tgaaaatgta ggcgtaaggg attcccggaa
      901 cattctgatc cggaaatatt gtgagctgca tcccgaccag atctttacca ttctgagtaa
      961 caacccctcc cttccttttg ccgacagcct gatcattctc ggcggaaagc ataatatccg
     1021 caaactgtat gattatgctt ccggcaattc ggctctttct tcgcggatcc gcaacagtcc
     1081 cgattccctg atccgcacgg ttgctaaaat agccgccagc cgcagcggtc agctttatct
     1141 tccttttctg gacaacctgc tccatcgtaa aattaccgtt gacgagatta ataaagtaaa
     1201 ggacgacaac ctgaattact atcgactgct ggtaaaaaca agactggatt atgtggaacg
     1261 catgctgcca ccccagcatg acaccgccgt ggaaatggaa tcccttaccc ggatgctgga
     1321 aagcaaggga aaggatgttt tcataagaga aataaatgcc ctgcacgaca agcccgcctc
     1381 ggtccggttc aagatcctgg acaagcttac gccggctgaa ttgtattacc tggtggtgct
     1441 gagtgaggat gaaatttata cctccagcta cctgggcgtg tatgaccgta tttttcaacg
     1501 gatgaatgtt ccccggggag acagcctgat catgcaaatg cacggcgact atttccggaa
     1561 attcataaaa atggcggcgg cttacaataa gctggatcat ttcctggcca ccatggacaa
     1621 gtacaatgca gccaccatca tgaaggcttt tattatcggg cttgaacgca ccaatgaaga
     1681 agacgccgtt gatgtggccg attcctacag tagcatattc gacaagaacc cttcgctcgc
     1741 agcctttgta ctgaatgagg tgaaatggaa ctataaacgc aatctgctca atgacgataa
     1801 aaagggtacc atcatctacc atctcctgga agccctgttt gaatctgccg ataccagcaa
     1861 tcataccgat ttatccgcca ggctgggtat cccgccggtt tacagcattg actatgactc
     1921 gctgaaagat gatagcgggc gggtgatcca gcaggtattt ttttatgggg acgaagacaa
     1981 ggacggacag cattcctacg atgattatct aagacttttc ctgcacaagc cggaatggaa
     2041 aattacgatg aacccaacca aagaatgggt gaccattcaa tcggtaaaag gcaagccggt
     2101 cataatcttt gccaaccggc cgctttacgg tgaagacgat ccggatgata aggcccagca
     2161 ccacctggat gattatctgt acgacaataa actgaagccc accatctata ttcaccgcgg
     2221 tcatagctat catgtccgct acaccctgca gcagattcag ccatccgcaa aaatagtggt
     2281 gctgggctcc tgcggagggt ataataatct cagcgaagta ctgagcatca gcagcgatgc
     2341 acacatcatt tcttcgaaac aggtgggtac aaaatccgtg aatgagccca tcctccaggc
     2401 cataaataat aacctgctgg aagggaacaa tattgactgg ttgcccatct ggaaagggct
     2461 gactgaaaaa ttccgtaaag acccggtcgc gcgcgagcgg ttcgatgatt atatcccgcc
     2521 ttataaaaac ctgggggtca tcttcattaa ggcataccgg aaagccatgg gagaagaaga
     2581 ttaaattttt tacctattac ttttttataa aatggctgac tccgtctcca ggaaaaaaat
     2641 attcgatttc agtctgctgc agcgtgtatt aagatatgcg gcaccgtata aaaaatggtt
     2701 ttacggctcc atcgcattat ccatcttgct ggccgttatt tcccccttgc gtcccttcct
     2761 gatacagtat acggtaaatc attatatccg gggtggcatt acgggtgatc cctccctaag
     2821 ggaaaggatg atcagtttga tcgtttatat cagtgtcgtg caaattggcc tgctgctcat
     2881 tgaaaccgta ttccgttttt tcttttcctt ccttacttcc tggctgggtc agaccgtggt
     2941 aaaagacctc cggatagccg tttataaaaa gattctcgga ctgaacctgt cacaatacga
     3001 taaaacgccg atcggaacgc ttaccacgcg aaccatcaat gatattgaag cgatcaatga
     3061 tatattttcg gagggtttta ttcccatcgt atccgattta ttatccattg tttccatcct
     3121 gctgtttatg tttctggcca actggcagct taccctggtc tgtctgatcc ctttcccctt
     3181 cctgatcctc tctacttatt tttttaagga agcggtgaac aaatcgttta tccgggtgcg
     3241 taatgcagta gctgccttaa acgcatttgt acaggaacat ataaccggaa tgcaaatcgt
     3301 tcaggccttt gcggccgagg accaggaata caaaagtttc cggaagatca attcggaaca
     3361 ccggaatgcc aatatcaggg ccatttttgc ctattccgtc ttttttccca ttgtggaaat
     3421 tgtgctggct gtttccaccg gcctgctggt gtggtggggt gccgaactgg tgctcgacct
     3481 cccgccggca caggcagccc gcctcaccgg ggagatcatt gccttctttc tgtacctgaa
     3541 tttattgttt cgtcccctgc gcctgattgc ggataaattc aataccctgc agatgggcat
     3601 ggtcgccagc gaacgtgtat tcagagtact ggataatgaa gaccacatcc gttcggaggg
     3661 agggtatgca ccggcacggc tcctggggaa aatagaattt gaccgggttt attttgccta
     3721 tacggatgag cgctatgtgc tgaagaatat ttcctttctg gttcagccca gcgaaaccct
     3781 ggccattgta ggccataccg gaagcggcaa gacctccatc atcagcattc tcaaccggct
     3841 gtattccatt cagaagggcc agatccggct ggacgatcgc gcgatcgaaa tgtacgacct
     3901 ggacgcttta cggtcatcca tcggcgtggt tttgcaggat gtattccttt tttcgggatc
     3961 cattctcgag aatatcacgc tgcgtaaccc cgatatccca tttgaaaagg tggtccaggc
     4021 agccaaactc atcgggatgc acgatttcat tctggaactc cccggccagt ataattataa
     4081 tgtgatggaa agggggagta cgctttctct cgggcaaaga caattactgt cctttatccg
     4141 ggcccttttg ttcgatccgt ccatcctgat cctggatgaa gcaacctctt cggtggacac
     4201 cggttcagag cggcttatcc aggaagccat cggaaaactg atagccgacc gcacctccat
     4261 cgtgatcgcc caccgcctct ccaccatccg gaaggccagc aagatcctgg tcctggacaa
     4321 gggcgagatt aaggaaacag gcacacacga agaactgatg gaaattcagg gattttatta
     4381 ccgtttgcac cagatgcaat ttgaagaaaa agtggatagc tgagaaaacc gggcagcttc
     4441 ccgcggccaa ccaaaaatac ccggtttttt ccgcaacaaa accacattcc ttcttgcaaa
     4501 ttcagccccc tcccttatct ttgcggcaaa atttggatac cgcatgatgc agggaaggtt
     4561 caaatcatta ttggtagcgg gtttcagcgt ttttttactc cttttttcag gggttctccg
     4621 ggcacaggaa aaccaggcgg aaaagggcgg taaaaagcac cagacttttg acgccaacga
     4681 ggtgatcttt aaccatgttc tggatgccca cgaatttcat tttttatcct accggagtct
     4741 ggaaggcagg gaacatcagg tgagcattcc cttgccggtc attttatatt cgccgcagcg
     4801 cggattttct tcctttttat cttcccggtt tcatcatggc gaagaggctt accaggggta
     4861 taagctcgcc ggcaataaga ttgttcccgt aaatgcggat ggttccgtgg acccctccgt
     4921 taaggtatat gatatttccc tgacgcgcaa cgtggtgcag atgatgatcg ccctcgccct
     4981 gctggttttc ctcctggtca ggattgccgg aaaatacaaa agcggtaccg gaaccacccg
     5041 ggcgccaacg ggcttccaga atttaatgga acccgtaatc acttttgtgc gggacgaagt
     5101 ggctgttccc aacctcggta caaaatacca gaaatacctg ccttacctgc taacggtatt
     5161 tttcttcatt ctcatcaata ccatggtcgg gctggttccc ggttctgcca acgtaaccgg
     5221 caatattgcg tttaccctgg tattgggtat gatttccttt atcgtgatca cggtgagcgg
     5281 gaacaaacat tattgggggc atattattaa tccgccggtc ccattcggga ttaaattcat
     5341 tatgatcccg gtggagatcc tgggaatatt taccaagccc tttgccctga ttattcgtct
     5401 ttttgccaat atggtggcgg gtcatatcat tatcatttgc ctggtttccc tgatctttat
     5461 tttcggggaa ctgaataaag gaataggatg gggattttcg cccgtatcga tcgggttcgc
     5521 ggtgtttatt taccttatcg aaatactggt ggcgttcatc caggctttta tatttgcaaa
     5581 cctgacggct gttttcattg gtcaggcaat tgaaaataat gaaacacata gcggcaacgg
     5641 acacgacgat gcggtgattg tttaacggaa acggactttt taaaccaata taaattacca
     5701 agtatggatt taggaaccct gttattgagt acaaccccct gggcaaatgc cggaggtgcg
     5761 atcggtgctg gtctggctgc cattggcgcc ggtatcggta tcggtcagat tggtaaaggc
     5821 gcggtggaag ccatcgcccg tcagccggaa gcatccaacg acatccgtgc caacatgatc
     5881 ctcgccgcgg cgctggtgga aggggttgca ctgttcggcg ttattgccgg tatcctggca
     5941 atgttcgttt agaacatcat tcgtactgca tccgaagtac aggacggagg atgcagtata
     6001 tttttaaagg aaacacccga tataaattat tttcatggat cttttaattc cttccttcgg
     6061 cctcattttc tggacgctgc tggcgttcgt gatcgtggtt atggtattga aaaagtttgc
     6121 ctggaaaccc attctgcaat ctctcaatga ccgcgaaaag accatttccg attctctggc
     6181 cacggctgaa cgcgtaaagg ctgaaatggc tcagatgaaa agtgaaaatg aagctctcct
     6241 ggccagggcc cgggaggaaa gagcactgat gctgaaagaa gcccgcgata caaaggataa
     6301 aatgatcagc gaagccaggg accaggcgaa aacagaagcc ggaaagatcc tgtcggatgc
     6361 ccaggtggcc atcgaacagc aaaaaatggc agccatcacg gatctgaaga atcagatggg
     6421 taacctcgtg atcgaagtgt cggaaaaaat tctgcgtcgt caactggaaa acaaatctga
     6481 acaggagaaa tatatcaggc agcttacctc cgaggtgaag ctgaactgac cgagtaattt
     6541 gaaacccatt tccggttttg taatcatcaa attttcaaat tgaaataatg ctcaatcccc
     6601 gcatagccgg cagatatgca aaatccctga ttgacctggc tgttgaaaaa gatcagctgg
     6661 aggccgtcta tcaggatatg gtctatttgc agaacctgaa ccaggctagc ccggagtttg
     6721 taagaatact taaaagcccg gtgatcaagg gagacaaaaa agaaaagatt ctcgaggccc
     6781 tgacaaagga taaaatgggt aatatcagtc aatcgtttaa ccgcctgctg gtaaaaaagg
     6841 gacgcgaggc gtatatcccg gaaatcgttt ctgcttttat tcagcaatac aaggattata
     6901 agggaataca tattgtttcc ctgaccacgg cggttccttt aactgaagaa tccaggtcgg
     6961 ctatcgttaa caggatcaac gaatccgctc aattgaaaga agtggaactc cggaccaaag
     7021 tagatccgga tattattggc ggatttatta ttgaagtgga tggccggtta atggatgcca
     7081 gtattctcta tgacctgaat accatcaaga aacaattcga aagcaacgat tttatatata
     7141 aaatccgcta gcagaaaaat aattcattct aaaaagaata tatggcagaa ataaaaccag
     7201 acgaaatatc ggcgatacta cgccagcagc tgagcaactt caacgtgtcg gccgatatgg
     7261 aagaagttgg cacggtgctc caggtggggg atgggatcgc gcgcgtttac ggcctcaaca
     7321 atgtgagcag cggggaactc gtggaatttg aaaacggggt cagggccatc gcattgaatc
     7381 tggaagaaga taatgtgggc gtcgtcctga tgggagaaag cggagatata aaagaagggg
     7441 caaaagtgcg ccgtaccggg aagattgctt cgatccaggt aggggaaggc atgctgggaa
     7501 gggtagtgaa taccctgggt ctgccgatag acggccgggg tccggtgcag ggtgaaaaac
     7561 acgaaatgcc gctcgaacgt aaggctccgg gagtgatctt ccgggagccg gttaaggaac
     7621 cgcttcaaac tggcataaag gccatcgatg cgatgatccc catcggccgc ggacaacgtg
     7681 aattaatcat cggtgaccgt cagacaggaa aaacagccat cgcgatagac accattatca
     7741 atcaaaaaga atttcacaaa gcaggtaaac cggtaatctg catatatgtt gcgatcgggc
     7801 aaaaagcatc cacggtggcc ggcatcatga aaacccttac ggatcatggt gcaatggaat
     7861 atacggtaat cgttgctgct tcggcttctg acccggctcc tctccagttc tacgcaccgt
     7921 ttgccggtgc ggcaatcggg gagttctttc gcgataccgg tcgccccgcc ctgatcattt
     7981 acgatgatct gtccaaacag gccgttgctt accgggaggt ttccctgctg cttcgccgcc
     8041 cgcccggaag agaagcttac ccgggggacg tattctatct gcatagccgt ttgcttgaac
     8101 gcgcagccaa aatcatcagt gatgatgcag tcgccaagaa tatgaacgac ctcccggaat
     8161 ccctgaaaag ccaggtgaaa ggaggaggat cgcttacggc cttgcctatc attgaaacgc
     8221 aggccggaga tgtttctgcc tatataccaa ccaatgtgat ttcgattact gacggacaaa
     8281 tctttctgga gggcaatctg tttaactccg gtatccggcc agccattaac gtaggtattt
     8341 cggttagccg cgtgggtgga aatgcccaga ttaaatccat gaaaaaagtt gccggtacat
     8401 tgaaacttga tcaggcgcta taccgggaaa tggaagcctt ctcaaaattc ggcggtgacc
     8461 tggatgcttc taccaagctg gtattggata agggagcccg taacgtggaa atcctcaaac
     8521 agccccaata ttctccggta gccgtggaga aacaggttgc gatcatttac ctgggaaccc
     8581 agggcctgct ccgcaatgtg cccgtgaagc ggatcaaagc ctttgaagaa catttcctga
     8641 tggaaatgga aaacaaattg ccggaggtgc tggcagagtt caaaaaaggg ggcctttcgg
     8701 atgatgccat tcgccggaca acggcattag ccaatgagct gattccgcag tacaaataaa
     8761 aaaattaaga cgtttgtgta atcgtggcgg tatagagcgg acggcggaca gatacccggg
     8821 cggagaagca tcaagactcc gttagctgtc tgctctgtac gcgccatttt gccatccgca
     8881 tccaaactat tccaagaata tttttcgaaa cacttgcatt tttgaaaaac gcttgcaatc
     8941 tttgtcctgt cagtcatccg taccaatctc ctgattaact ccccttgcca attttcctga
     9001 atccgattta ctgtttctga ttttatataa tacactttcc aatttcttgg tagaaggcct
     9061 ttgtttccaa taactatggg atagtattaa ttcccatgcg ctaaaataag ttatgaaaca
     9121 aattcacctg cgccggttga aaggagagag tgggcgtatg attcgaaacg gtttgacggt
     9181 tctttttttc ttaagccaat acaccgggct gcacgcccag tccctggttc ctgaactggt
     9241 tttcatgaat ccgcaattga agacgggatc gggttgcccg gacgccggaa aggacggagc
     9301 ggtatatatt ttttcaaatg ttggtgtggg gatcgatgcc ctggtaagta ttcagggcag
     9361 aagcagttca aaagtaaact tatccgccat tgacctgaaa ggacctgagc aggatccggc
     9421 caaaggcacg ggatacgaca acgcctggca gcctaaagtg agttatggaa acggtgttgc
     9481 cacggctcat agcaactggt ggatggaatt taagatttca tttgtaaaac atgacaatcc
     9541 cgatcagtct gtatcggtaa atcagttctt tgtttccgga ttggatattg acggggacgg
     9601 cagccgcctg catgaatttc agtcgtatta caaaatgcag tatttcacgc tggaaaaaaa
     9661 tacggatatg gcttccagtt cagtgagtgg ctgtttgaat gatccgcagc tggaaggaaa
     9721 gcgattcgac gggccggtaa agaattacga aggcataaca accagtgcat cggaggtgat
     9781 ggtgagtaat ttttataaca atgccagcag cctggtggtg agagtgggcg ctgaaacagg
     9841 ctctgcgggc agtcaggcga cggaccggat gtatgcgctc tggttcaaat ccctgaccta
     9901 cgacgttccg gtgagtgccc cccttccgct tacccacatt gcctttaatg ctcaattgcg
     9961 gaataataag gatgtgcatt tgaactggac aaccagtatg gagcagaaca tcagtcattt
    10021 tacgttgcaa cgaagcgtga atgggaaaga ttttgatgat gccgctgttc tttttaccga
    10081 agggaacagc gcgggcaaaa agaattatca gcttacggat catctgccgc cggtaagc
//
LOCUS       DASYCU010000086         2466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4081655, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000086 DASYCU010000000
VERSION     DASYCU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4081655"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..744
                     /gene="msrA"
                     /locus_tag="VGM24_01720"
     CDS             85..744
                     /gene="msrA"
                     /locus_tag="VGM24_01720"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY4154105.1"
                     /translation="MLSAILFSLFTTASCYSSNTQKPVMNDETIPAGTKTDTATFATG
                     CFWCTEAIFQELKGVLKVTSGYSGGTVPNPSYEEVCSGTTGHAECLQVIYDPKKITYD
                     ELLEVFWESHDPTSLNRQGNDEGTQYRSAIFYHDAEQKEKAEQYKAKLNQSGAYNKPI
                     VTEITPFSKFYPAENYHQDYYRLHGSQPYCSFVIRPKVEKFEKVFKDKLKTNDKLSTG
                     K"
     gene            complement(753..1355)
                     /locus_tag="VGM24_01725"
     CDS             complement(753..1355)
                     /locus_tag="VGM24_01725"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY4154106.1"
                     /translation="MIKKKKRNSWFIFLLIVIAWTGCRENPRPVKTMGKGGETIYSNQ
                     TSLQTELQLPDGTKLMMDSATIIRLRAGFNQGNRDLYLEGEAIFRVDPSGVKPFVVHT
                     RALLTAVLDTSPAIFKIYGYPASPGEEVDLFAGNLKVTKSYHSETDNEPVILRAGEMA
                     MVNTDIDLMEKEKLDSAELRSGMLSPKGICSPVFGGTNKD"
     gene            1478..>2466
                     /gene="sucC"
                     /locus_tag="VGM24_01730"
     CDS             1478..>2466
                     /gene="sucC"
                     /locus_tag="VGM24_01730"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY4154107.1"
                     /translation="MNLHEYQAKELLKKYHVPVQEGIPVDTANAAEEAYKQIKVQTGN
                     SFAVVKSQIHAGGRGKGKIRGTEQRGVAVAKNAEDVRNFASNMLGGTLVTIQTGETGK
                     VVHKVLVAQDVYYPGANPTKEFYLSVLLDRAKGQNVIMYSTEGGMDIEEVAHKTPDRI
                     FKEWVFPGGALQPFQARKIAFNLGLSGEAFKNCVKFVSNLYNAYVGLDCSMLEINPLF
                     KTSDEKIIAVDCKMNLDDNALMRHPDLEGMRDISEEDPTEVEAGKYNLNFVKLDGNVG
                     CMVNGAGLAMATMDMIKLSGGEPANFLDVGGTANAQTVEAGFRIILKDPKVKA"
BASE COUNT          694 a          593 c          603 g          576 t
ORIGIN      
        1 taaaaggctt cgattgtcat atatattgca atccggagcc ctcatctctt cgtatattac
       61 caaagaaaaa gaatgaaaat taccatgtta tcagctatcc tgttcagcct ctttacaacg
      121 gcttcctgct attcatcaaa cacacaaaaa ccagttatga acgacgagac cattccggcc
      181 ggaacaaaaa ccgataccgc aacctttgcc accggctgct tctggtgtac agaagccatt
      241 tttcaggagt tgaaaggcgt cctgaaagtt acttccggct attccggcgg aacggtgccc
      301 aaccccagtt acgaggaggt ttgttccggc accacggggc atgcggaatg ccttcaggtg
      361 atttacgacc ctaaaaaaat aacctatgat gaactcctgg aagtattctg ggaatcacat
      421 gacccgacct cgctcaaccg tcagggtaat gatgaaggca ctcaataccg gagcgctatt
      481 ttttaccatg atgcagaaca gaaagaaaaa gcagaacagt ataaagcaaa actgaatcag
      541 agcggggcgt acaataaacc cattgtaacg gagattacgc cgttcagcaa attttacccc
      601 gccgaaaatt accaccagga ctattaccgc ctgcacggat cacagccgta ttgcagcttt
      661 gtgatccggc cgaaggtgga aaagttcgaa aaggtgttca aggataagct caaaacaaac
      721 gataagctca gtaccggaaa ataagattca gatcaatcct tatttgttcc gccaaagacg
      781 ggtgagcaga tccccttcgg agacagcatg cccgacctta gttctgcact gtctaatttt
      841 tccttttcca tcaggtcgat gtcggtattg accatggcca tttcaccggc ccggagtatg
      901 accggctcat tgtctgtttc cgaatgataa gatttggtca ctttcaggtt tcccgcaaac
      961 agatccactt cctccccggg gcttgcggga taaccatata ttttaaaaat agcaggcgat
     1021 gtatccagaa cggctgtcag caaggcccgg gtatggacca caaacggttt tacaccgctg
     1081 ggatccactc tgaatatggc ttctccttcg aggtacagat ccctgttccc ctggttaaaa
     1141 cctgcccgta gacgaatgat cgtggcggag tccatcatca gctttgtccc atccggcaat
     1201 tgcagctccg tctgcaagga agtctgattg ctataaatcg tctctcctcc tttgcccatc
     1261 gtttttaccg gccgggggtt ttctctgcaa ccggtccagg cgataacaat caataaaaaa
     1321 ataaaccagg aatttctctt cttctttttt atcatttcag gtccgcttaa gatacagcgc
     1381 cgggtgatgc cgggcggaaa agctaaagat gattgcaagg actatcgcag caaatatcta
     1441 tttttgcaat taattatatt taataaaatt tttgccgatg aatcttcacg aataccaggc
     1501 caaggaactc cttaagaaat atcacgtgcc cgttcaggag ggcattccgg tagacacggc
     1561 caatgcggct gaagaagcgt acaaacagat aaaggtgcag accggcaatt catttgccgt
     1621 cgtgaaatcc cagatccatg cgggcggccg ggggaagggc aagatcaggg gaaccgagca
     1681 gcggggcgtg gcggtcgcca aaaatgcgga ggacgtccgg aattttgcat cgaacatgct
     1741 gggtggcacg ctggtgacca ttcaaacggg ggaaaccgga aaagtggttc ataaagtgct
     1801 cgtggcacag gatgtttatt atccgggcgc caatccaacc aaagaatttt atctctctgt
     1861 gctactcgac cgggcaaaag gacagaatgt gatcatgtac agcacggagg gcggaatgga
     1921 tattgaagaa gtggcgcaca aaacgcctga tagaattttt aaggaatggg tattcccggg
     1981 tggggccctc cagccctttc aggcccgcaa gatcgcgttt aacctaggcc tttcgggtga
     2041 agcgttcaaa aattgcgtaa agttcgtcag caacctctat aatgcgtatg tgggactcga
     2101 ttgcagcatg ctggaaatta acccgctttt taaaaccagc gatgagaaaa tcattgccgt
     2161 ggattgcaaa atgaacctgg atgacaatgc cctgatgagg caccctgatc tggaaggcat
     2221 gcgggatatc agcgaggaag atcccacgga agtggaagcc ggcaaataca acctcaattt
     2281 tgtaaaactc gacggaaatg tgggttgtat ggtgaacggg gccgggctgg ccatggcgac
     2341 catggacatg atcaaactca gcggcgggga acctgccaat ttcctggatg taggaggtac
     2401 cgccaatgca caaacagtag aagcaggctt tcgtatcatt cttaaagatc ccaaggtaaa
     2461 ggcgat
//
LOCUS       DASYCU010000087         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4373061, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000087 DASYCU010000000
VERSION     DASYCU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4373061"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VGM24_01735"
     CDS             <1..1148
                     /locus_tag="VGM24_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154108.1"
                     /translation="YKPADRPLNQPGMDPEDPGSYSRHARMMWNLAAFFGNTKVDTAL
                     LSISNRPKQSGRGSMSMYENGNEEDGWWTGKKYCNPVEYFAQSTADWDGDEGRLGRRA
                     GIRNADPHSLLMMSGLIGLDTNRVKVYKFLSENLREDHQFPWPGGIQYHYYCTRNGRG
                     ISPEADSLRWRLTQARNCSRRIVPAAECFLGENGYDKGQASRQGTPLLPGYSVSQSQG
                     IMLLRSINATFFSGFDAYILYWLRDSYPEDAPDLYLTSGILRQMPDGKTMVYPGWYYI
                     STMVNQLGNYTADRVISEKGNVWIYKYRHRLFHDSVAYFVYSPTVIGAKIRQYALSVG
                     NITDDIAREIIFKDDSEQGSESRLTATGSRVIIPVEEKPLLILCRER"
     gene            1232..>1641
                     /locus_tag="VGM24_01740"
     CDS             1232..>1641
                     /locus_tag="VGM24_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY4154109.1"
                     /translation="MDLVWKLPYIFYPVLTLLSCSYSSKATKKLYQQALDKQPYDAII
                     VPGVPFEIGQWSRVMKGRIYWSKFLFDRGITRNIIYSGSAVYTPYIEADIMAGYAIAL
                     GIPKEHVFRETHAEHSTENVYYSYKLARSLGFKK"
BASE COUNT          442 a          393 c          402 g          404 t
ORIGIN      
        1 gatacaaacc ggcagacagg cccctgaatc aacccggaat ggatcccgaa gaccccggaa
       61 gctattcccg tcacgcaagg atgatgtgga acctggcagc ttttttcggg aacacaaaag
      121 tagataccgc cctgctctcc atctccaacc ggccaaaaca atccggacgc ggatccatgt
      181 ccatgtatga aaacggcaat gaggaggacg gatggtggac cggaaaaaaa tattgcaatc
      241 cggtggaata ctttgcccaa agcaccgccg actgggatgg agatgaagga aggcttggaa
      301 ggcgggccgg catccggaac gcagatccgc actccctttt aatgatgagc gggttgatcg
      361 ggctggacac gaaccgggtg aaagtatata aatttctttc ggaaaatctg cgggaggatc
      421 accagtttcc ctggccgggc gggattcaat atcattatta ttgtacccgg aatggcaggg
      481 gaatttctcc ggaagccgat tcccttcgct ggcgtctgac acaggcccgc aactgcagca
      541 gaagaatcgt acctgctgcc gaatgttttc tgggagaaaa tggttatgac aaaggacagg
      601 cgtcccggca gggaacacct cttcttcccg gatactccgt atcccagagt cagggcatca
      661 tgttgctccg ctccatcaat gccacatttt tttccggttt cgatgcgtat atcctgtatt
      721 ggcttcggga cagctatccc gaagatgctc ccgatcttta cctgacgagt ggtattttaa
      781 ggcagatgcc ggacggaaaa accatggttt atccgggctg gtattacatc agcacaatgg
      841 ttaatcaatt gggaaactat acagcggacc gggtaatatc ggaaaaaggg aatgtctgga
      901 tatataaata ccgccaccgc ctgtttcacg attctgttgc ctatttcgtg tattcaccga
      961 ccgtcattgg agcgaaaatt cggcaatatg ccctttctgt aggaaatata acagatgata
     1021 tcgcccgtga aatcattttc aaagacgatt ctgaacaggg atctgaaagc aggctgaccg
     1081 ccacagggag ccgtgtaata attccggtgg aagaaaaacc gctgctgatt ttatgccggg
     1141 agcgctgaaa ttgcagatcg cgcatggttg acggatcatc agtcacccgc tattttcccc
     1201 attcattcct tatcttcatc ctttaaagca tatggattta gtctggaaac tcccttatat
     1261 attctacccg gttctgacct tattgtcctg ttcttattct tcaaaagcca caaaaaaact
     1321 ctatcagcag gcactggaca aacagccata cgatgcgatc atcgttccgg gcgttccttt
     1381 tgaaatcgga caatggagcc gggtaatgaa aggccggata tactggtcta aatttttgtt
     1441 tgatcgggga ataacccgca atattattta ttccggctcc gccgtttaca cgccatatat
     1501 tgaagcggat atcatggccg ggtatgcgat cgctttaggc attccaaaag agcatgtttt
     1561 cagagagaca catgccgaac acagtacgga aaacgtatat tactcatata aacttgccag
     1621 gagtctgggg tttaagaaga t
//
LOCUS       DASYCU010000088         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1181473, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000088 DASYCU010000000
VERSION     DASYCU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181473"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VGM24_01745"
     CDS             <1..686
                     /locus_tag="VGM24_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154110.1"
                     /translation="IKYLNPLTKAVDGDGVYDGDRCQANVLFHNNIRYPVYLRLGYIF
                     YKDKPYLDRTYQLLNPAGTQTLPVNTYMALIQGLLITKIPYTIPWKGALFSYIQPNGQ
                     NMKIKKGADPEGVWSALYNPNTMEDIIGSMQAPNSSYTISSNKSFAVGKSFYHSLYYS
                     DTLNYSHVHADVAICQCMAHGAWEIGSGLLPNDYPIPPEKTSEEITRRFGFPQGSPIM
                     NDKRSEKKH"
     gene            705..>2950
                     /locus_tag="VGM24_01750"
     CDS             705..>2950
                     /locus_tag="VGM24_01750"
                     /inference="COORDINATES: protein motif:HMM:NF037611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEY4154111.1"
                     /translation="MKKLMLLFFLFAFRGTSHASAQQDGKPIWSIGKADHSAAEFALA
                     PDGFKDFVGHDFGYEDKFFLVGFSKEKNDFPYVLPGPVDTWGGTWPTAGWRTNEVNIL
                     FGVQDLPSNGDYKLIIRLSDYAKKFLPLLKVQINDQDERIQLDAPGYDVAGQPRPRQN
                     EPYVDSAALKGDLSSATPRTLEIPIKAGVIRRGGNRVCITVVQGSWIKFDEVGLEGPG
                     AVHLRTPGQMFVRSVKPADYELETNGKKGQPLLVNVQYLKGSPQLSVALDDKIIFKEN
                     VEKGNYVFEALMPAVTASRRSRYKILENGKLLEEGIVERSRQKLQTLADYVDTRMGTA
                     HSRWMIAPGPWMPFSMVKMSPDNQNAGWQAGYQPTYESIGTFSHIHEWTMAGLGIFAS
                     NGKLKTKIGDELKPGSGYRSGIDKKTEEAPIGYYKAQLTDYHIKAEVTATTRCGFERF
                     TFPADRDSARILVDLHVPAEYDYQLKEISVKKVSDYRIEGFAHQLTPRVWSNDAGQDY
                     TIHFVIEFDRPIKEMGSWINDQVQYADTLEAKDIKNAGFFLQFDAKRDPVVQVRSAIS
                     LVSTENAAMNLKKEITQPFGWDFAAVRKNQLNVWNDIFNRVKITTTNRLEKMRFYNSM
                     YRSVCSRNTWSDLNGEWRGTDGKVQKLKSSDDVALGCDAFWNTFWNLNQVWNLVTPEW
                     SNRWVNSQLAMYDAYGWLAKGPAGMNYVPVMVAEHEIPLMVSAYQMGIRNFDANKLLE
                     AAAKMQTTP"
BASE COUNT          812 a          658 c          795 g          685 t
ORIGIN      
        1 aaataaaata tctgaatccg cttaccaaag ccgtagatgg ggatggcgtc tatgacgggg
       61 accggtgtca ggccaatgtg ctcttccata ataatatccg ctatccggtt tacctccggc
      121 ttggctatat attttataag gacaaaccct atttggacag gacctaccag ctccttaatc
      181 cggcgggtac ccaaaccctg ccggttaaca cctatatggc cctcatccag ggacttttga
      241 tcaccaaaat accttatacg attccgtgga agggagcgct gttctcctat atacaaccga
      301 acgggcagaa tatgaaaatc aaaaagggag cggacccgga aggcgtctgg tctgcgcttt
      361 ataacccaaa cacgatggag gatatcatcg gatcgatgca ggcgcccaat tcttcttaca
      421 ccataagcag caataaaagt tttgcggttg gtaaatcgtt ttatcattct ttatattaca
      481 gcgatacgct gaattattcc catgtacatg ccgatgtggc gatttgtcag tgcatggccc
      541 atggcgcctg ggaaatcgga agcgggttgc ttccgaatga ttatcccatt cctccggaaa
      601 aaacgagcga agaaatcacc cgccgctttg gttttccgca gggatctccg ataatgaatg
      661 ataaacggtc agagaaaaaa cattaactat ttaagtcaag tacaatgaag aagctgatgc
      721 tgttattttt cctgtttgcc ttccggggga catcccatgc cagcgcacag caggacggaa
      781 aaccaatctg gagtatagga aaggcggatc attccgccgc tgaattcgcg ctggcgcccg
      841 atgggtttaa agactttgtt gggcatgatt tcggatatga ggacaagttt ttcctggtcg
      901 ggttttccaa agaaaaaaac gattttccct atgtgctgcc cggtccggtg gatacctggg
      961 gcggtacctg gcctacggcg ggatggcgga ccaatgaagt gaatattttg tttggcgttc
     1021 aggatttgcc gtcaaacggg gactataaac tgatcatccg gttatcggat tacgcgaaga
     1081 aattcctgcc cctgcttaaa gtgcagatca atgaccagga cgaaagaatt cagctggatg
     1141 ctcccggata tgatgtagcc ggccagccga ggccgcggca aaacgaaccc tatgttgact
     1201 ctgccgcact gaaaggggat ttatcttcgg cgactccccg gacgctggaa attccgatca
     1261 aggcaggcgt gatcaggagg gggggaaacc gggtgtgcat caccgtggtg cagggctcgt
     1321 ggataaaatt tgatgaggtc ggcctggaag ggcccggggc cgtgcattta agaacgccgg
     1381 ggcagatgtt tgttcgtagt gtaaagccgg ctgactatga gctggagacg aatggaaaaa
     1441 aaggacagcc tttgctggta aatgttcaat acctgaaagg ttcgccccaa ctcagcgtcg
     1501 cattggacga taaaataatt tttaaggaga atgttgaaaa aggaaattat gtttttgagg
     1561 cattgatgcc ggccgtgacc gcttccaggc gaagcaggta taagattctg gaaaacggaa
     1621 aactgctgga agagggcatt gtagagaggt caaggcaaaa gttgcaaacg ctggccgatt
     1681 acgtggatac ccgtatggga accgcgcatt cccgctggat gatcgccccc ggtccctgga
     1741 tgcctttcag tatggtgaaa atgagcccgg ataatcagaa tgcgggatgg caggccggat
     1801 accagcccac ctatgagagt attggcacgt tcagccatat tcatgagtgg acgatggcgg
     1861 gcctgggcat attcgccagc aatggaaaac tgaaaaccaa aataggagac gagctgaagc
     1921 ctggatccgg ttatcgttcc gggatcgaca aaaaaacaga agaagcgccg atcggatatt
     1981 ataaggcgca acttaccgat taccatatca aggccgaagt gacggctacc acgcgctgcg
     2041 gttttgagcg ttttaccttt ccggcggatc gcgacagcgc gcgcatcctg gtggatttac
     2101 atgttcccgc tgaatatgat tatcaactga aagagattag cgttaagaaa gtgagtgatt
     2161 accggataga aggatttgct catcagctta ccccgcgcgt ttggagtaat gatgccgggc
     2221 aggattatac gattcatttt gtaattgaat ttgacaggcc aataaaagaa atggggagct
     2281 ggatcaatga ccaggttcaa tatgccgata cgctggaagc gaaggatatt aagaatgccg
     2341 ggttctttct gcagtttgat gcaaaacggg atccggtagt gcaggtgcgg tcggccatat
     2401 cactggtcag cacagaaaat gccgctatga atttaaaaaa ggaaataacc caacctttcg
     2461 gatgggattt tgctgccgta cgaaaaaacc agctgaacgt atggaatgat attttcaatc
     2521 gcgttaaaat cacgacaacc aaccggctgg aaaaaatgcg tttttacaac tcgatgtacc
     2581 ggtctgtttg cagccgcaat acctggagcg atctgaacgg cgaatggcgc ggcaccgacg
     2641 ggaaagttca aaaattaaaa agcagcgatg atgtagcatt gggatgcgat gctttctgga
     2701 acactttctg gaatctcaat caggtatgga acctggtcac gccggaatgg agtaaccgct
     2761 gggtcaattc ccaactcgcc atgtacgatg cctacgggtg gctggcaaaa ggtcctgccg
     2821 gaatgaatta cgtgccggtg atggttgcgg aacacgaaat acccctgatg gtctctgcct
     2881 accagatggg aatacgaaat tttgatgcga ataaactgct ggaggcagct gccaaaatgc
     2941 aaacgacccc
//
LOCUS       DASYCU010000089         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3792661, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000089 DASYCU010000000
VERSION     DASYCU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792661"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VGM24_01755"
     CDS             <1..1455
                     /locus_tag="VGM24_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY4154112.1"
                     /translation="VKGRAGPDGKWKVRFPGLAKNTSFDINITTGEGEEKVIRNAVAG
                     QVWLCSGQSNMGMPVSASDVKEKLKDSDVHEIRFFLAQNDPSATELHRLTGHWVICTT
                     QTIGSCSAVGLSFAWNLHQHLRQPVGLIINAVGGTPIESWTPLTTVKEKAYNIHVFQE
                     RAQWQRDRNVYEKEYQLKLKNREIAVQQAREEGRPLPPKIYPPFALRENWNPGSLYNS
                     LVCPVRDFVFKGIIWYQGESNANYPFVYRYQLADMIRCWRKLFNKKKLPFYIIQLPDY
                     QCADDWAVIRESQASVGSLNDTRLIVTLGLGDSLNIHPVRKMEVGRRVSLQVLRKEYG
                     YDLVASGPRLKKMEITGEKIRLSFDCSGSLIKSSDGRPLRNMEISDDGLVFFPAQVRI
                     DRNTLLVWSDKIKHPGMVRYAWKNVPEPINFVNQDGLPAPPFFSGGKEQHILMEKNER
                     KNNLGSADQGRRKIPQIIRDFYSGVRKGTEAGPA"
     gene            1506..2213
                     /locus_tag="VGM24_01760"
     CDS             1506..2213
                     /locus_tag="VGM24_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HEY4154113.1"
                     /translation="MQLKRSVINKSIDIARHVIEHFGVCLPEFSGWSPEDWNRAGADY
                     NEIRHCMLGWDVTDFGSNDFYNIGRVLFTLRNGRSGNPDYPKEYAEKLLIDPENQRAP
                     AHFHVSKREDLICRGGGNILVQLTKADAAGKRSPNDFTIQVDGCSVKMKPAGIVRLEP
                     GQSICIPPGTIHQFWAEEGTGWKMDGVGYTISTEVSSVCDDINDNIFIETAGIRFPEI
                     EEDEARQYLLCNEYPVI"
     gene            2237..>3733
                     /locus_tag="VGM24_01765"
     CDS             2237..>3733
                     /locus_tag="VGM24_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY4154114.1"
                     /translation="MKRKREFCTAPAEIERGRIFRMAGFALLFCILTGFDAAGGITTA
                     KLNVQKDKSIVSGLHAEFRNGQVFLTWNEASGNSKNLRVYISGEPINARSLSSAKLLT
                     AQLEPHSANDWYDDTAECWRAKGPAHGWIIEAGRAPLDRTNGLFVHTVLQRDPSYAYF
                     AVLGEDENAAKLRSGINSLKKPVSISTGNIQAIWQSAGTQPVANGKPLMLFLHSHEGR
                     PAGELTQLFFGDSSMGWREGLPFKFKVSIRPDAVLLEPFDRVWINRRMSGDEARANNG
                     MYDTLYHEIESWWYGTNSKINDAAALSTGTATNYTENWLLWALDWVQQHYGTDPQKVY
                     AFGASMGTGVLRLVLRNPARFASVDLLVPILDPFGEGNVGNRMKSRVGEPQSICSDGI
                     ELSARLNTFNAIRSAKTDLPPIVIRVGRTDQSVFWIRKPEFMRTVQGEKQALFAGWDN
                     GTHSTAMRKRYEGFPNWFDFKWYISHFALNKSYPVFTACSLDDHPGNGL"
BASE COUNT         1024 a          834 c         1016 g          859 t
ORIGIN      
        1 gttaaaggcc gcgcgggccc tgacggaaaa tggaaagtac ggtttccggg tttggcaaaa
       61 aatacatcct tcgacatcaa tataacaacc ggtgaaggag aagaaaaagt gatccggaac
      121 gcggtggccg gccaggtatg gctttgcagc ggccagtcga atatgggaat gccggtttcc
      181 gccagcgatg tcaaagaaaa gctgaaggat tccgatgttc atgaaatcag atttttcctt
      241 gcgcagaacg acccatctgc gacggaactg caccggttaa cgggtcattg ggtgatctgt
      301 acaacgcaaa cgatcggcag ttgttccgct gtcggattga gttttgcctg gaatcttcat
      361 cagcatttac ggcagccggt tggtttaatt ataaatgcgg tcggaggaac gcctatagaa
      421 tcctggacgc cgctcacaac cgtaaaagaa aaagcctata acatccatgt ttttcaggaa
      481 agggcacaat ggcaaaggga caggaacgtc tatgagaagg aatatcagct caaattaaaa
      541 aacagggaaa tagcagtgca acaggccagg gaagagggaa gaccgctgcc tcccaaaatt
      601 tacccgccct ttgcgttgcg cgaaaactgg aatcccggga gtctttataa ttctctggtc
      661 tgtccggtcc gcgattttgt ttttaaaggc attatctggt atcagggtga atccaatgca
      721 aactacccct ttgtataccg gtaccagctt gccgatatga tccgctgctg gcgaaagctc
      781 tttaataaga aaaagctgcc tttctatatt atacagctac cggattatca atgcgccgat
      841 gactgggccg tgatccggga aagccaggct tccgtcggtt ctctgaatga tacccggctg
      901 attgtaacgc tcggtttggg cgactccctg aatattcatc cggttagaaa aatggaagtg
      961 ggacgaaggg tttcgttaca ggttctccga aaagaatatg gatatgatct ggtagcatcg
     1021 ggaccccggc tgaaaaagat ggaaataacc ggagaaaaga taaggctctc ctttgattgc
     1081 tccgggagcc tcataaaatc ttccgatggc cggccgctgc ggaacatgga aatttcggat
     1141 gacggattgg tatttttccc ggcgcaggtc cgcatcgacc ggaatacgct gctggtctgg
     1201 agcgataaaa taaaacatcc cgggatggtc cggtatgcct ggaagaatgt tccggaaccg
     1261 ataaattttg taaatcagga tggtcttccc gctcctccat tcttttctgg tggcaaagag
     1321 caacatattt taatggaaaa aaatgagcgg aaaaacaatt tgggatccgc agatcagggg
     1381 cgacggaaga ttccgcagat catccgggac ttctattccg gggtgcgcaa ggggactgaa
     1441 gccggtccgg cgtaacaccg gaaaagcgga tagaatcgct aaaattatta acctaaaaca
     1501 gaacaatgca acttaaacgg tcagtgataa ataaaagcat agacatcgcc agacacgtaa
     1561 tagaacattt tggagtatgc ctgccggaat tttccggatg gagtccggaa gattggaaca
     1621 gggccggagc ggattataac gaaattcgtc attgtatgct gggttgggat gtaacggatt
     1681 ttggaagtaa cgatttttat aatatcggca gggtattatt tacccttcgc aatggaagat
     1741 ccggaaatcc cgattatcca aaggaatatg cggaaaagct tttgatcgat ccggagaacc
     1801 agcgggcgcc ggctcatttc cacgtctcca aacgggaaga ccttatttgc aggggtggag
     1861 gaaatatatt ggttcagctg accaaagccg atgcggccgg caagagatcg ccgaacgatt
     1921 ttacgataca ggtggacgga tgtagcgtaa aaatgaaacc ggccggaatc gtccggctgg
     1981 agccgggaca aagtatttgt attccgccgg gaacgataca tcaattttgg gcggaagaag
     2041 gtaccggctg gaaaatggat ggtgttggct atacgatcag cacggaggtt tccagtgttt
     2101 gcgatgatat caacgacaat atatttattg aaacggcggg aatccgtttt ccggaaattg
     2161 aagaagatga ggccaggcaa tacctgcttt gcaatgagta tccggttatt tgaaaacgct
     2221 actaaacata cgaatgatga aaaggaaacg agaattttgc acagcgccgg ctgaaataga
     2281 aaggggaagg atattccgga tggcaggctt tgccctcctg ttttgtattt tgaccggttt
     2341 tgatgcggcc ggcggtatca caacagcgaa actaaatgtt caaaaggata agagcatcgt
     2401 atccggcctg cacgcagagt tccggaacgg acaggttttt ctgacctgga atgaagcttc
     2461 cggaaatagt aaaaatctgc gggtttatat ttcaggggaa cctataaacg cccgcagtct
     2521 atcttccgcg aagctgctga ccgctcaact ggaaccgcat agtgccaatg actggtatga
     2581 cgacacggct gaatgctggc gtgcaaaggg accggcacat ggatggatta tcgaagcagg
     2641 ccgtgcgccg ctcgaccgga ccaatggctt gtttgttcac acggttttac aaagagatcc
     2701 atcctatgct tattttgcgg ttttgggaga agatgaaaac gcggcaaagc tgagatccgg
     2761 aatcaattca ttgaagaagc cggtttccat tagcaccgga aacattcagg ccatttggca
     2821 gtcggccgga acccagcccg tggcgaatgg caagcccctt atgctctttc ttcattcgca
     2881 tgagggccgg ccggccggcg aactcacaca actttttttt ggagacagca gtatgggctg
     2941 gcgcgaggga ctgcctttta aattcaaggt aagcatccgc ccggacgcgg tgctgctgga
     3001 gccgttcgac cgggtatgga tcaatcgcag gatgtcgggc gacgaagcca gggccaataa
     3061 tggaatgtac gatacccttt atcacgagat cgaaagctgg tggtatggta caaacagcaa
     3121 aatcaacgat gcggccgctc tttcaaccgg tacggcgaca aattacaccg aaaactggct
     3181 tctctgggcc ctggactggg ttcagcaaca ctatggaacg gacccgcaaa aagtatatgc
     3241 attcggagcc tccatgggca ccggcgtact acgactggtg ctcagaaacc cggcccggtt
     3301 tgccagtgtc gatctgctgg tgccgatact tgatccattt ggagaaggta atgtaggcaa
     3361 ccggatgaaa tcccgggtgg gagaaccgca aagtatttgt tccgacggca tcgaattaag
     3421 cgcacggctc aatacattca atgcgatccg gtctgccaaa acagacctgc cgccgattgt
     3481 gatccgcgta ggacggaccg accaaagtgt attctggatt cggaaaccgg aatttatgag
     3541 aaccgttcag ggggaaaaac aggcgctctt tgccggctgg gataatggta cacacagtac
     3601 cgctatgcgg aaacggtatg agggatttcc aaactggttt gactttaaat ggtacatcag
     3661 tcattttgct ttaaataaga gctatccggt gttcactgcc tgctcgctgg atgatcatcc
     3721 cggcaatggc ctc
//
LOCUS       DASYCU010000090         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2922673, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000090 DASYCU010000000
VERSION     DASYCU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2922673"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VGM24_01770"
     CDS             complement(<1..1040)
                     /locus_tag="VGM24_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY4154115.1"
                     /translation="MGKLTGFLEYKRELPKKRPVQERIKDYKEFVNPFEPAQLNQQAA
                     RCMNCGIPFCSHGCPLGNVIPEFNDAIYRNAWLEAYEILSSTNNFPEFTGRICPAPCE
                     SSCVLGINQPAITIEEIEKHIIEIAFEKGFVKAKEPNVKTGKKIAIVGSGPAGMAAAA
                     QLNSAGHAVTVFERDDAPGGLLRYGIPDFKLEKWVIDRRIALLEQEGVVFRCNANVGV
                     NIHINDLMREYHAVVLAGGSTIPRDLPIAGRELNGVHFAMDFLRQQNKRVSSTAIREA
                     DILASNKNVIVIGGGDTGSDCVGTSNRHGAKSITQFELLPAPPKERTQFMPWPTYPMI
                     LKTSSSHEEGCE"
     gene            complement(1062..>3450)
                     /locus_tag="VGM24_01775"
     CDS             complement(1062..>3450)
                     /locus_tag="VGM24_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY4154116.1"
                     /translation="PEQLKKNYVKAVNEGLLKVFSKMGISTLQSYQGAQIFEIIGLNR
                     SVVDKYITGATSRIEGMGLDEIARETLGKHLFAFSKKDIPVDRLPAGGVYQWKRKGEF
                     HLFNPSTIHFLQYSTHMNDYASFKKYSKLINDQMEKAATLRGMLSFKRNRPSISLDEV
                     EPEENILRRFATGAMSFGSISHEAHSTLAIAMNRIGGKSNTGEGGEDEFRFEPLPNGD
                     SMRSSIKQVASARFGVTSYYLTQADELQIKMAQGAKPGEGGQLPGHKVDEWIGKVRHS
                     TPGVGLISPPPHHDIYSIEDLAQLIFDLKNANRKARISVKLVSKAGVGTIAAGVAKAK
                     SDVILISGHDGGTGASPVSSIRHAGLPWELGLAESQQTLVRNKLRSRVVLQTDGQLKT
                     GRDIAIATLLGAEEWGVATAALVVEGCIMMRKCHTNACPVGVATQNPELRKRFTGNAD
                     HVVNLFKFLTRELREIMAELGFRTINQMVGQIECLEVNDNVRHWKAKKLNLSPILFKD
                     PASANTGIHNMEEQDHGLNELLDWKLLAVAKPAIESGQKVFASFSVKNTDRTIGTILS
                     NEISKKFKSKGLPQDSIHFKLNGTAGQSFGAFNTAGVTLELEGDANDYFGKGLSGARL
                     IVYPAADAGFVPEENFIIGNVAFYGATSGEAFIRGKAGERFAVRNSGADAVVEGIGDH
                     GCEYMTGGRVVILGSTGRNFAAGMSGGIAYVYDVGGKFASLCNPDMVELDPLDEEDAS
                     AMKEMIAKHYAYTASTVARFVLDDFENQLRNFVKVFPTDYKKVLLEKKSRVKANS"
BASE COUNT          757 a          940 c          803 g          950 t
ORIGIN      
        1 ctttcacatc cctcttcatg ggaagaagag gtttttaata tcatcggata ggtgggccag
       61 ggcatgaact gcgttctttc ctttggtggc gctggtagta attcaaactg cgttatggat
      121 tttgccccgt gccggttaga ggttcccaca cagtcgcttc cggtgtctcc gcccccgatc
      181 acaattacat ttttattgga agcgagtatg tccgcttccc tgatcgccgt tgaacttacc
      241 cgtttgttct gctgccttaa aaaatccatg gcaaaatgaa ccccattcaa ttccctgccc
      301 gctatgggta agtcgcgggg aatggtagat ccccccgcca gcacgacagc atgatattcc
      361 cgcatcaggt cattgatatg aatatttacg cccacattgg cattacagcg gaatacaacg
      421 ccttcctgtt ccaggagggc aatccttctg tcgatcaccc atttttccag tttgaaatcg
      481 ggaattccgt aacgaagcag tccgcccggt gcgtcatctc tttcaaaaac ggtaaccgca
      541 tgcccggcgc tgttgagctg tgccgcggcc gccattcccg ccggaccgga gcccacaatg
      601 gctattttct tacccgtttt tacattcggt tcttttgctt tcacaaaccc tttctcaaag
      661 gcgatttcaa tgatatgctt ttcgatctct tcgatggtga ttgccggctg gttaatgccc
      721 aatacgcaac tgctttcgca gggagccgga caaattcttc cggtaaactc cggaaaatta
      781 tttgttgaag aaaggatctc gtatgcttcc agccaggcat tgcggtaaat ggcgtcattg
      841 aattccggga tcacattccc aagcgggcat ccgtggctgc agaatggaat tccgcagttc
      901 atgcagcgtg cagcctgctg attcagctgc gccggctcga acgggttcac aaattcttta
      961 tagtctttta ttctttcctg aacgggacgc ttttttggaa gctcacgttt atattccagg
     1021 aaacctgtca gttttcccat gtcttaatgt ctcttaatag tttatgaatt ggcctttacc
     1081 cgggacttct tttccagcaa cacttttttg taatccgttg gaaatacttt cacaaaattc
     1141 cggagttgat tttcaaaatc gtccagtaca aaccgggcta ccgtgctggc cgtataggca
     1201 taatgtttcg ctatcatttc tttcatcgcg gaggcgtctt cttcatccag cggatccagt
     1261 tcaaccatat cgggattgca aagggaagca aatttgccgc ccacgtcgta aacataagcg
     1321 atgccgccac tcatcccggc cgcaaaatta cgcccggtgg atcccaggat gaccacccgg
     1381 ccgccggtca tgtattcaca accatgatct ccgattccct ccacgacggc atctgcccct
     1441 gaattccgga cggcaaagcg ttctcctgct ttcccgcgga taaaggcttc gccggacgta
     1501 gctccgtaaa aggccacgtt tccgataatg aaattttctt ccggaacaaa cccggcatcc
     1561 gcagcgggat atacaatcaa ccgggcgccc gataaaccct ttccgaaata atcattcgca
     1621 tctccttcca gctccagggt cacccccgct gtgttgaagg cgccaaaact ttgtccggcc
     1681 gttccattga gcttgaaatg tatactgtcc tggggcaatc cttttgactt gaattttttg
     1741 gatatctcat tggagagaat agtgccgatg gtgcggtccg tattttttac actgaaagag
     1801 gcaaatactt tctgccccga ttcaatggcc ggttttgcca ccgccagcaa tttccagtcg
     1861 agcaactcat ttagaccatg gtcctgttct tccatgttat ggattccggt attcgcagaa
     1921 gcagggtcct tgaataatat cggagagagg tttaatttct tagccttcca gtgtcttaca
     1981 ttgtcattta cttccaggca ttcaatctgt cccaccattt gattaatggt ccggaagcca
     2041 agttcagcca tgatctcgcg caactcccgg gtcaggaact taaacaggtt taccacgtga
     2101 tccgcattgc cggtaaatct ttttcttaat tccggattct gggtggctac ccccaccgga
     2161 caggcgtttg tatggcattt ccgcatcatg atgcaacctt ccaccaccag ggcggccgtg
     2221 gcaacgcccc attcctctgc accgagaagg gtggctatgg caatatcgcg gccggtcttt
     2281 aactggccat ctgtttgtaa taccaccctg ctgcgcaact tgtttctgac cagggtctgc
     2341 tgggattcgg cgagtcccag ttcccagggg agtccggcat ggcgaatgga actgaccggc
     2401 gatgcccccg ttcctccgtc gtgacccgat atcaggatta catccgactt tgcttttgca
     2461 acgccggccg cgatggtccc cacacccgct tttgatacca gcttgacgct gatacgcgct
     2521 ttccggtttg catttttcag gtcgaagatc agctgggcaa ggtcttcgat ggaataaata
     2581 tcgtgatggg gcgggggaga gatcagtccg acgcccggcg tggaatgcct tactttcccg
     2641 atccattcat ccactttatg accgggaagc tgtccgcctt cacccggctt ggccccctgg
     2701 gccattttga tttgcagctc atcggcctgg gtgagataat agctggtaac accaaacctg
     2761 gcggaggcca cctgttttat cgaagaccgc atggaatccc cgttcggcag gggttcaaac
     2821 cggaattcat cttccccgcc ttcaccggta ttgcttttcc cgccgatgcg gttcatggca
     2881 atggccaggg ttgaatgggc ttcgtgggaa atggaaccga aactcatggc gcctgttgca
     2941 aaccgcctca ggatattttc ttccggctcc acttcatcca gggaaataga cggccggttt
     3001 cttttaaaac tgagcatacc gcgaagggtt gccgcttttt ccatctgatc gttgatcagc
     3061 ttcgaatatt ttttaaacga agcataatca ttcatgtggg tggaatactg caggaaatgt
     3121 atggtggacg gattgaataa atgaaattcc cccttccgtt tccattgata tacgccaccc
     3181 gccggcaggc gatccaccgg aatatctttc ttactgaaag caaataaatg ttttccgagg
     3241 gtttcccggg cgatttcgtc caggcccatt ccttcgatac gggaagtggc gccggtaata
     3301 tatttatcca ctacggaccg gttcaatccg atgatctcaa agatctgtgc gccctggtat
     3361 gactgcaagg tggagatccc cattttcgaa aacactttca gcaatccttc attaactgct
     3421 tttacgtaat ttttcttgag ctgctccgga
//
LOCUS       DASYCU010000091         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5098655, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000091 DASYCU010000000
VERSION     DASYCU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5098655"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VGM24_01780"
     CDS             complement(<1..126)
                     /locus_tag="VGM24_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY4154117.1"
                     /translation="MAISRRGIILMNLGSPDSTAVKDVRKYLNEFLFDSRVIDYSW"
     gene            299..1531
                     /gene="hemA"
                     /locus_tag="VGM24_01785"
     CDS             299..1531
                     /gene="hemA"
                     /locus_tag="VGM24_01785"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY4154118.1"
                     /translation="MSRNPVAELSKFFVVGVNYKKTDTLIRGSFAVSSAQYEQILQNA
                     AGFGVHDLFVLSTCNRSEIYGLAENPGQLVRLLCSQTEGSEDTFNELAYCKEGLNAIR
                     HLFDVAAGLDSQILGDYEIVGQIRQAVKFARERKFIHTFLERTLNQVLQSSKDIKNQT
                     ALSDGTVSVAFAAVHYIRDNIQQVKTKNILLLGTGKIGSNACKNMVDYLGTTKITLIN
                     RTNEKAEDLASALNLRYASMDQLPEEIKRADIILVATNAADPVIRAEQLESLDSNKLI
                     IDLSIPCNVDKSVALLSSITLIDVDSLSRTKDETLQRRAGEVPKAEAIIAGHMRELLD
                     WHEMRKHVPVLREVKIKLQEIYNSPLFEQPVPARTLSMATDQKIQRVLNGMASKMRRE
                     NQRGCQYIQAINEFIAGG"
     gene            1543..2463
                     /gene="hemC"
                     /locus_tag="VGM24_01790"
     CDS             1543..2463
                     /gene="hemC"
                     /locus_tag="VGM24_01790"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY4154119.1"
                     /translation="MSKTFRIGTRDSQLAIWQATRVQHLLKSHGISAELVPVKSEGDL
                     DLETPLYAMGVQGIFTRSLDQALLNQRIDLAVHSMKDVPVQLPKGIVEAAVLKRDSPF
                     DILVLREETADSFSDRHSPVRIASSSLRRRSQWLHRFPQDGVEPIRGNVNTRLKKLAE
                     SDWKGAIFAAAGLERIGLRPSNAIELDWMLPAPAQGALLVVCRGNDDELLRACTKLND
                     DHTAFSAKIERDFLSALMGGCSTPVGALAEIENEEVYFRGNLTSIDGKKQVSIEKYIS
                     VELAASLGTSAAQELLEDGASEILQEIKHA"
     gene            2456..>3027
                     /locus_tag="VGM24_01795"
     CDS             2456..>3027
                     /locus_tag="VGM24_01795"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY4154120.1"
                     /translation="MPDRVCTILSTGSLMFGESPVPANRNAGIPGIIEEVPFIKIILN
                     TDENTRGQILSYSRQSLAAVFTSANAVRAVTAALDDIPDWDIYCVGNETRKQVACFFG
                     STRILGAAGNAEGLAERIVPQEGEKKIVFFCGDQRRNVLPDKLNEKGIEWQELVVYQT
                     RHTPVRLTKNYDAILFFSPTAVASFFSINMV"
BASE COUNT          805 a          760 c          760 g          702 t
ORIGIN      
        1 ccaggaatag tcaatcaccc ggctatcaaa gaggaattca ttcaggtatt ttctgacatc
       61 ttttacggcc gtggaatccg gtgaccccag gttcatcaag ataatacccc gtctcgaaat
      121 tgccatttgc aaatgtaatt acagaataca acagatattc aacccgccgg cattttatgg
      181 cccgggggag gcctgccggc cctatccatg aattgtacaa atgacattat tatgacacta
      241 aaatgctgag ttaagccacc tgccaagtaa ttttgcgttt ctactattaa acctccggat
      301 gagcaggaac cctgtagctg agctttctaa attttttgtg gttggtgtca attataagaa
      361 aaccgatacc ctgatccgcg gttcatttgc ggttagttca gcacaatatg aacaaatcct
      421 gcaaaatgcg gccgggttcg gagttcatga tctttttgtt ttatccacct gcaaccggtc
      481 cgaaatttac gggctcgcag aaaatcccgg acagctcgtc cgtctgcttt gttcccagac
      541 cgaaggatca gaagacacat tcaacgaatt ggcctattgc aaggagggac taaatgccat
      601 ccggcacctc tttgatgtgg ctgcgggcct ggattcccag atcctgggcg actatgaaat
      661 cgtgggtcag atccggcagg cggtgaaatt tgcccgggag agaaaattca tacatacttt
      721 cctggaaagg accctgaacc aggtattgca atcatccaag gacattaaga accaaaccgc
      781 actgagcgat ggaaccgttt ccgtggcgtt tgcggccgtt cattatatcc gggacaatat
      841 tcagcaggtc aaaaccaaaa acatcctttt gttaggcacc ggaaagatcg gcagcaacgc
      901 ctgtaaaaat atggtggact acctgggtac caccaagatc accctgatca accggaccaa
      961 tgaaaaagca gaagatcttg cttccgcatt gaatttgcgg tatgcctcca tggatcaact
     1021 gccggaagaa atcaaacggg cggatattat tctggtagcg accaatgcgg cagacccggt
     1081 tatccgcgca gaacagctgg aaagcctgga ctccaataaa ctgatcatcg atttgtccat
     1141 accctgcaat gtagataagt cagttgctct tctttcttcc attaccctga ttgacgtgga
     1201 cagtctctcc cggacgaaag atgaaacgct gcagcgccgc gccggcgaag tgcccaaagc
     1261 agaagcgatc attgcaggtc atatgcgtga actgctggac tggcatgaaa tgcgcaaaca
     1321 cgttccggtt ttgagggaag taaaaataaa acttcaggaa atttacaatt cccctttgtt
     1381 tgagcagccc gttcccgccc ggacactctc catggcgaca gatcagaaaa tacaacgcgt
     1441 attaaacggg atggcttcta aaatgcgtcg cgaaaatcaa cgcggctgcc aatatatcca
     1501 ggccatcaac gagtttatcg caggaggata aaccactcaa tcatgtctaa aacgttcagg
     1561 atcgggaccc gggacagtca gctcgcaatc tggcaggcca cccgcgttca gcatttatta
     1621 aaaagccatg gaatatcagc cgaactggtt ccggtaaaaa gcgaaggcga cctggacctg
     1681 gagactcctt tatatgctat gggtgttcag ggaatattca cccgcagcct ggatcaggcc
     1741 ctgctgaatc aacgcattga cctcgccgtt cattccatga aagatgttcc ggtacagctt
     1801 cccaaaggaa ttgtggaagc cgccgtgctg aaaagggata gccccttcga tattctggtt
     1861 ctccgcgaag aaacggcgga ctctttttca gaccgccatt cccccgtgcg gatcgcctcc
     1921 agcagcctcc ggagaagatc gcagtggctg caccgttttc cccaggacgg ggtggaacca
     1981 atccgcggaa atgtgaacac ccgtttaaaa aagctggcgg agagtgattg gaagggagcc
     2041 atttttgccg cggccggact ggaacggatc ggcctgcgtc catccaacgc gatcgaactg
     2101 gactggatgc ttcctgctcc ggcacaaggg gcgctcctgg tcgtctgccg cggaaatgat
     2161 gatgaacttc tacgggcctg tacaaaactc aatgatgacc acaccgcgtt cagcgccaaa
     2221 atagaaagag attttttatc tgccctgatg ggcggctgtt ccaccccggt cggcgccctg
     2281 gccgaaattg aaaatgaaga agtttatttt cgcgggaacc tgaccagcat cgacggaaaa
     2341 aaacaggtga gcattgaaaa atacatttct gtggaactgg ccgcatccct tggcacgtcc
     2401 gccgcacagg agttgctgga agacggggca tctgaaatac ttcaggaaat aaaacatgcc
     2461 tgatcgcgta tgtacgatat tatccaccgg ttccctgatg ttcggggaat ccccggtacc
     2521 ggcaaaccgg aatgcgggga tcccgggcat tattgaagag gtccccttta taaaaataat
     2581 tttgaatacg gatgaaaata cgcgcgggca gatcctttcc tattcccgtc agtccctggc
     2641 ggcggttttt accagcgcca acgccgtgag agccgtgacc gccgccctgg atgatattcc
     2701 ggattgggat atatattgtg taggcaacga gacccggaaa caggttgctt gcttttttgg
     2761 ttcgacccgc atcctgggcg ctgcaggaaa tgcggaagga cttgccgaaa gaatcgttcc
     2821 gcaggaaggg gaaaagaaga ttgttttttt ctgcggggac cagcgcagga atgtattgcc
     2881 ggataaactg aatgaaaagg gaatcgaatg gcaggaattg gtggtctatc aaacccggca
     2941 tacccccgtg cggctgacca aaaactatga tgccatttta tttttcagtc cgacggcagt
     3001 ggccagtttt ttctctataa atatggt
//
LOCUS       DASYCU010000092         4823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4374904, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000092 DASYCU010000000
VERSION     DASYCU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4374904"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1323)
                     /locus_tag="VGM24_01800"
     CDS             complement(244..1323)
                     /locus_tag="VGM24_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY4154121.1"
                     /translation="MWERIRSYLTFTRKERLGVLVLLFIILVLFIAPYLFGPETGSPD
                     PQAYQKYQEGIRKFQSAGREGPAEKTNTNGINGRRSGPFTDQLNKPTGPLDFVSEKTG
                     SGEMKNRPASFYFDPNRISADDWRRLGLPDKLILTISHYIQKGGIFRTPADLKKLYGL
                     KTEEYERLFPYVRIREPPNQEFHSAAAYHPSKRNRNFFERGKDSSTSRYDKPYLPAWQ
                     MHAYSIKKPEDLDINQADSSIWCRFPGIGIKLASRIVHFREKLGGFYSVDQLHETFGL
                     PDSTFQQIRPFLHISSPGLQKIDLNTADREILQAHPYIGWKLAREIILFREQHGGFRS
                     AGELQQLVQMDPEKYKKLEPYLEVK"
     gene            complement(1407..2120)
                     /locus_tag="VGM24_01805"
     CDS             complement(1407..2120)
                     /locus_tag="VGM24_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154122.1"
                     /translation="MKKTIWITVLMFGIMGYSFGQSAIEFIPMGGYTFPDKLSFDHSF
                     GRIEDGYNVGGSFQFNFNRHFGLELMYNRVDAQAKMYDYGSSMSQAPFYQTRSATNYI
                     MLGPVSSVQVPGSPVQLFFGAMLGAAIYTPGPDDFSSNASFAWGLQTGTNIYFTRRLG
                     LRLSARLLSATASDHGSGYYFGSFGESHGGYFSNPPIYQFGFNAGLVIGLGRALPEYR
                     RPARPQRRPAPHPRRYYYY"
     gene            complement(2202..2984)
                     /locus_tag="VGM24_01810"
     CDS             complement(2202..2984)
                     /locus_tag="VGM24_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4154123.1"
                     /translation="MNNPSLKITFLGSGTSSGVPMIACPCPVCHSADSKDKRLRSSIL
                     VESAATRLVVDTTPDFRYQMLRADVRKLDAVLFTHPHKDHIAGLDDIRAFNYFSGKPM
                     DIYANEMTQEVIIREFPYAFSDFHYPGIPEIRLNTIATDPFVIGDIEVIPIQVWHLKM
                     PVLGFRFGDFTYITDANRVDEPERKKIRGSKRVVINALRMEKHISHFSLPEAIDFIRE
                     LQIPEGYFTHISHQLGKHGDVQRDLPSGMNLAYDGLVLTPDP"
     gene            complement(3117..3380)
                     /locus_tag="VGM24_01815"
     CDS             complement(3117..3380)
                     /locus_tag="VGM24_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154124.1"
                     /translation="MKYNTGTLDKIEKLLHATGYLIRYERGNFQSGYCILEEKKVLVL
                     NKFLPVEGRINTLIDIIPQLKIDAATLPAESRKIFENLLKAEV"
     gene            complement(3441..>4823)
                     /locus_tag="VGM24_01820"
     CDS             complement(3441..>4823)
                     /locus_tag="VGM24_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HEY4154125.1"
                     /translation="GLETLAKIKEANQQIPVVLITKNETENLMDEAIGRQITDYLIKP
                     VNPNQVLLSLKKIIDNKRLVAETTTTAYQQQFRELFMALNDNPDYNEWMDLYKKLVYW
                     ELEMEKSNSPEMQEVFQTQKQEANTEFFKFISKNYSKWVGTKQNGAPTMSHSLMRNKV
                     FPNLEKGVPMFFVLIDNLRFDQWKAIQPVFAENFRIQAEETFYSILPTATQYCRNAIF
                     AGLMPLEISALYPSLWKNDEEEGGKNLAEEEFFRSQLKRLKMDHLKTSYTKVLNHQAG
                     QDLVNNMHNLMGNDLNVIVYNFVDMLSHARTEMEVLKELASDEASYRSITNSWFEHSP
                     LHQALKKIADKKITIILATDHGSVRVKTPYKVIGDKQTTTNLRYKHGRNLNYEAKEVL
                     AFRDPKEAGLPVPTINSSFIFARGDGFLCYPNNYNYYANYYRNSFQHGGVSMEEMIVP
                     VIKMTGKGGS"
BASE COUNT         1183 a         1182 c         1151 g         1307 t
ORIGIN      
        1 tcccattcca taacatgtgg cttaatatac cggagcgcaa aatccctggc cgtagcggca
       61 gcctgacggg aaagttcttc taattcaaaa ttcataagtt ggtgttcaga ataaaaaagg
      121 caacaacctg aggttgccgc ctaaacagca aacaatcgtt agcgaatacg taagactcat
      181 gtgactgtga tgcaatttac gaatttttat tctaaatact aacagatgtt agtttttggc
      241 ccgctatttt acctccagat agggctccag ttttttatat ttttcaggat ccatttgaac
      301 gagttgctgc aactctccgg cggatctgaa tccaccatgt tgttcccgga acaggatgat
      361 ttcccgggcc agtttccacc cgatataagg atgggcctgc agtatttccc ggtcagccgt
      421 attcagatct attttctgca gcccgggtga gctaatgtgt aagaacggcc ggatctgctg
      481 aaaagttgaa tccggcaagc caaaggtttc gtgcaactga tcaaccgaat aaaacccacc
      541 cagcttctcc cggaaatgaa cgatgcggga cgccagtttt attccgattc ccggaaagcg
      601 gcaccagatg gaagaatccg cctggttgat atcgaggtcc tccggttttt ttattgaata
      661 agcatgcatc tgccaggctg gcaaatacgg tttatcatac cggctggtgg acgaatcctt
      721 tccgcgttca aaaaaatttc tgtttctttt ggagggatgg taggctgctg cagaatgaaa
      781 ttcctgattt ggcggctccc ggatccggac ataaggaaac agccgttcat attcttccgt
      841 tttcaaacca tacaattttt ttaaatcggc tggcgtccgg aatatgccgc ctttctgaat
      901 ataatggcta atggtcagaa tgagtttatc cggcaggcca agccgtcgcc agtcatcggc
      961 ggatatccgg ttgggatcga aataaaaaga agccggccgg tttttcattt cgccggatcc
     1021 ggttttttct gaaacgaaat ccaatggacc ggttggctta ttcagttgat ccgtaaatgg
     1081 accggagcgc cggccgttaa tcccgtttgt attggttttt tcagccggtc cttcccggcc
     1141 cgccgattga aatttccgaa tgccttcctg atatttctga taggcctgcg gatccggaga
     1201 acccgtctcc ggcccgaaaa gataaggagc tatgaacaag accaggatga tgaacagcaa
     1261 aaccaacacg cccaggcgct ccttccgcgt aaatgtcagg tatgaacgaa tcctttccca
     1321 cataatcttt ttattaaaga taaatgggta ttgcgcccgg cacaataccc acttagtgga
     1381 aaaatggaat ccgtctccgg aaaaaatcaa taataataat atctccgcgg gtgaggggcc
     1441 ggccggcgtt gtggtctggc cggacgcctg tattcaggca atgcccgtcc cagcccgatc
     1501 accagcccgg cattgaaacc aaactgatag atcggcggat tggaaaaata tccgccatga
     1561 ctttctccga aactcccgaa ataatatcct gatccatgat ccgaggcggt ggccgacaat
     1621 aagcgggcgc tcaaccgcag gcccagcctc ctggtaaaat aaatatttgt ccccgtttgc
     1681 aagccccagg caaaactcgc attgctggaa aaatcatccg ggcccggtgt atagatcgcc
     1741 gctcccagca tggcgccgaa aaacagttgc acgggagatc cggggacctg cacggaggag
     1801 accggaccga gcattatata attggtggcc gaccgggtct gataaaaggg cgcctgactc
     1861 atggaactcc cgtaatcata catctttgcc tgcgcgtcca cccggttata catcagttcc
     1921 aggccaaaat gccggttgaa attaaactgg aatgaaccgc cgacattgta cccgtcttca
     1981 atacggccga aagaatggtc aaaactgagc ttatccggaa atgtatatcc tcccatcggg
     2041 atgaattcga tggccgattg tccgaaggaa tatcccatga ttccgaacat caaaacggta
     2101 atccagatcg tttttttcat attgtttgtt ttgcggtgtc ttggacacag gcaaaacgaa
     2161 aaagtttaag gttttgattg ttaaaggcat gctaaaaagc atcaaggatc aggcgttagg
     2221 acaagcccgt cataggccag gttcattccg gaaggaagat cccgctgcac atccccatgt
     2281 tttcccagct ggtggctgat atgggtaaaa tatccttcgg gtatttgcag ttcccgtata
     2341 aaatcaatgg cttcggggag gctgaaatgg gaaatatgct tttccatccg gagggcattt
     2401 atgacaaccc tcttgctacc ccggatcttt ttcctttccg gttcatccac ccggttggcg
     2461 tcggtgatat aagtgaaatc gccgaaacga aatccaagca cgggcatttt taaatgccac
     2521 acctggatgg ggatcacttc aatatctcct atcacgaaag gatcggtggc aatcgtattc
     2581 aggcggatct ccggaatccc ggggtaatga aagtctgaaa acgcataggg gaattcgcgg
     2641 atgatcactt cctgtgtcat ctcatttgca taaatatcca tgggcttccc ggaaaaataa
     2701 ttgaaggcgc ggatatcgtc gagcccggca atatgatcct tatggggatg tgtaaataag
     2761 accgcatcca gtttgcggac atctgcgcgg agcatctggt aacggaagtc cggcgtagtg
     2821 tccaccacca gcctggtggc ggccgattca acgaggatgc tggaccgcag acgtttgtct
     2881 ttggaatctg cggaatgaca aaccgggcag gggcaggcaa tcatgggaac tccgctgctc
     2941 gtacccgatc caagaaatgt aatttttaaa gagggattat tcacacactg aaaatacttt
     3001 aaataagctg atcccgggtc tgtccgctga agtttccgga taccggtatt ctttcagccc
     3061 gtttatcccg ttgaaagcaa aatttttaag gggtaataag taccggaagt ataagatcat
     3121 acttccgcct tcagcaggtt ttcgaagatc ttccggcttt ccgcgggaag cgtcgcggcg
     3181 tctatcttta gttggggaat gatgtctatc agggtgttga tccttccttc caccggcagg
     3241 aatttattca gcactagcac tttcttttct tccaggatac aataaccgct ttgaaaatta
     3301 cctctctcat accggatcag gtacccggtt gcatgcagca gcttctcgat cttatccagt
     3361 gtgccggtat tgtatttcat ggttcaaagt tctttattcg gctccaaaaa aagtcaggag
     3421 tttatccaca gcttactccg tcaggagccg cctttccccg tcatttttat caccggcaca
     3481 atcatttcct ccatgctcac cccgccgtgc tgaaagctgt tccggtaata atttgcgtaa
     3541 taattatagt tattgggata acagaggaac ccgtctcccc tggcaaagat aaaggatgaa
     3601 ttgatggtgg gtaccggcag ccccgcttct ttggggtcgc ggaaggccag cacttccttt
     3661 gcctcataat ttaaattgcg tccgtgttta tagcggaggt tggtggtggt ctgtttatcg
     3721 ccaatcactt tatacggggt tttcacgcgc acgctgccat gatcggttgc gagaatgatc
     3781 gttatttttt tatcagcgat ctttttcagg gcctgatgaa gaggcgaatg ttcaaaccag
     3841 ctgttggtaa tgctccggta actcgcttca tcgcttgcga gttctttgag cacttccatt
     3901 tcggtccggg catgactgag catgtccaca aaattataca cgatcacatt caggtcattg
     3961 cccatcaggt tatgcatatt attcaccagg tcctgccccg cctgatgatt cagcactttg
     4021 gtataggatg ttttcaggtg atccattttc aaccgcttca gctggctacg gaaaaattct
     4081 tcttccgcca ggttttttcc gccttcctct tcatcattct tccaaaggga aggatatagc
     4141 gcactgatct ccaagggcat cagcccggcg aaaatcgcat tccggcaata ctgggtagcc
     4201 gtcggaagaa tgctgtaaaa tgtttcttcg gcctgtatcc ggaagttctc ggcaaacacc
     4261 ggctggatgg ctttccactg gtcgaaccgc aggttgtcga tcagtacaaa gaacatcggc
     4321 acccctttct ccaggttcgg aaatacctta tttcgcatta aggaatgcga catcgtggga
     4381 gctccgtttt gtttcgtacc cacccatttg gaataattct ttgagataaa tttgaagaac
     4441 tcggtattgg cttcctgctt ctgggtttga aaaacttcct gcatctcggg actgttgctc
     4501 ttctccattt cgagttccca gtacaccagc tttttgtaca ggtccatcca ttcattataa
     4561 tccgggttgt cgttcaacgc catgaacaat tcgcggaatt gctgctggta agcggtggtg
     4621 gtggtctctg ccaccagtct tttgttgtca atgatctttt tcaggctaag caatacctgg
     4681 ttcggattta ccggtttgat caggtaatcg gtaatctggc ggccaatggc ctcgtccatc
     4741 agattttccg tttcgttctt ggtgatcagg accaccggga tctgctggtt ggcttccttg
     4801 atcttggcca gggtttcgag acc
//
LOCUS       DASYCU010000093         4655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3504714, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000093 DASYCU010000000
VERSION     DASYCU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504714"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VGM24_01825"
     CDS             complement(<1..1105)
                     /locus_tag="VGM24_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154126.1"
                     /translation="MKFPFVLSSLLIFCSLRSFSQAGPVGLFDRQQDIGYVKHAGSTS
                     YDEATQTYTIKGAGANIWFNEDQFHYSYKKLGGNFILTADFAFVGDTAGSTGHRKMGW
                     MIRESADADAASINATSHIDGLTVLQWREIKGAFMRDPADEIFFPKKGLQTIQLERKG
                     KRIIMRMANPGEPLQWVGEHIIPDMPDSVLAGVFICSHDSNALAEARVWNVRIDGPVE
                     NQYQPNPQLAKPSSAEVLGSRLEIMNVFDGKRKVIYESAGRFEAPNWMPDGKKLLFNE
                     GGSLFTIPIEGGAREKLNTGSANRINNDHGISFDGKTLAISNSREGLPGGGSSVYVLP
                     LTGGEPRMLTEKTPSYWHGWSPDNKEVAFVAQRG"
     gene            complement(1161..3950)
                     /locus_tag="VGM24_01830"
     CDS             complement(1161..3950)
                     /locus_tag="VGM24_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY4154127.1"
                     /translation="MKSFLSFSFIFFLYPVALSAQNRPVTQVKLSHFFLQSSAVVFTG
                     GDTLSLPGYPSPDYWFPVTVPSTVLTGLVQNKIYPDPYSGMNNMLIPDASDSFNHQYH
                     LEQYSYLPNDPNPWKKPYWYRTVFSVPASDAGKRFQLIFKGINYRAEVWVNGKRIADS
                     SLMVGMFAEYSLNISSEIRPGKENGLAVKIYPLDYPGLPAHPQLNALGDFYANGGPTG
                     DIGKNVTMLSSVGWDWIPAVRDRNMGIWLPVYLRTSGHTVISHPQITTDLPALPDTSV
                     ARIHLHLTLANDSHTRQEGNLKITITPENFKGQALSFTRPASVDGNGSLGIALGPEDV
                     RALTIHRPTLWWPNGYGQPNLYRIRIQFVQPAGISDDTSFLFGIRTVSSKTEMVNGWV
                     RRDFYVNGKRVHLVGGAWVPDMMLNRDSVRYDYELHLCRNANVNLVRIWGGGLGETDD
                     FYNLTDRYGLLVWQDFWITGDTHGGFKGSADWPMETHVFVDNVVSTIYRIRNHPSLLV
                     WTGGNEGHAREELYNAMRDNVATLDGTRPFIPCSSGFSKAPKEWKGSWPDDKPAGVYS
                     GGPYSWVGEQQYFKLADRGRDWVFKDETGLPSQPPYNSLIKIIPDLVPDKDLPYPLNN
                     SWGYHDACSGNGKYDIYFKAMESRFGKPESLKDFSVKMQLMNASGYRAIFEAAGHKLN
                     ETGGVMLWKLNAAFPSVVWQIFDWYLEPNAGYYFMQNACEPVHIQLNYDDSSVAVVNR
                     SYIPRPGLGWHVEIFGLDGKPFFKQSGKLNSDTTSVKQVLTLNNILQKAQGISFVVLN
                     LTDKQGNPVSHNVYWLSPDNDFHTLTEMPVTKLETRLVHAKRTGAENQWTFEIRNNTR
                     QLAFFVNPQLWNDQGEIMPTFWSGNYFSLSPGEKMVLTAACPAKLSGQTPLLKLEGWN
                     VPEEKIGLGPAVPGK"
     gene            complement(3984..>4655)
                     /locus_tag="VGM24_01835"
     CDS             complement(3984..>4655)
                     /locus_tag="VGM24_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter TolR"
                     /protein_id="HEY4154128.1"
                     /translation="PVNTGNIKNNNNDHVISFDGKMLGLSSSVRALGGSIIYTVPIDG
                     GSPKQITPKGPSYLHGWSPDGKFLVFCGERNGEFDVYKIPAEGGKEIRLTQTKGLDDG
                     PEYSPDGKYIYFNSVRSGSMQIWRMKPDGTDPEQVTDDGFNNWFAHISPDGKWIVFIS
                     FLKEEVQPGIHPPYKHVYLRLMPFGGGPAKVIAYFYGGQGSINTPSWSPDSKRIAFIS
                     NSVLK"
BASE COUNT         1124 a         1225 c         1143 g         1163 t
ORIGIN      
        1 ttccccgctg cgctacaaaa gcaacttctt tattgtcggg actccagcca tgccagtaag
       61 aaggtgtttt ttccgtgagc atgcggggct ctccgccggt cagggggagc acataaacgg
      121 atgaacctcc gccgggcagt ccttcccggg aattactgat ggccagggtt ttcccatcga
      181 aggaaattcc gtgatcatta ttgatccggt tggccgatcc ggtatttaat ttttcccggg
      241 caccgccttc aatgggaata gtaaaaaggg aacctccctc attaaacaat aattttttgc
      301 cgtccggcat ccagtttggc gcttcaaacc gtccggctga ttcataaata acctttcttt
      361 tcccgtcaaa aacattcatg atctccaacc ggctgcccaa cacttccgca gatgaaggtt
      421 tagccagctg cggattgggc tgatactgat tttcaaccgg tccgtcaata cggacattcc
      481 atacccgggc ttcggcaagc gcattggaat catgggaaca gatgaatact ccggccagca
      541 ccgaatcggg catgtccgga atgatatgtt ctccaaccca ctgaagcggc tcccccggat
      601 tggccatgcg cataatgatc cttttaccct tgcgttccag ctggatggtt tgaaggccct
      661 ttttcggaaa aaagatttca tccgcggggt cccgcataaa agcccctttt atttcccgcc
      721 attgcaatac cgtcagcccg tcaatatgcg aggtggcgtt aatactggcc gcatcggcat
      781 ccgccgattc ccggatcatc cagcccatct tacgatgacc ggtagaaccg gcggtatctc
      841 cgacaaaagc aaaatcggct gtgagtataa aatttccccc taatttttta tagctgtaat
      901 gaaactgatc ttcgttgaac cagatattcg caccggcccc cttaatcgta tacgtctggg
      961 tagcttcatc gtatgaggtt gatccggcat gtttaacata cccgatatcc tgctggcgat
     1021 caaagaggcc aaccgggccg gcctgggaga atgaacggag gctgcaaaaa ataagcaggc
     1081 tgcttaagac gaaaggaaat ttcatggaac tccgttttta tgcttaaaaa atacttcggt
     1141 atgagaatga agggaacaat ttattttccc ggaacggctg ggccgagccc tattttttct
     1201 tccggtacat tccatccttc cagcttaagc aggggcgtct gccccgataa tttagccgga
     1261 caggcagctg tgagcaccat tttttctccc ggagaaagtg aaaaataatt ccccgaccag
     1321 aaggtcggca taatttctcc ctgatcattc catagctggg ggttgacaaa gaaagccagc
     1381 tgacgggtgt tgttccggat ctcgaaagtc cattgatttt ctgccccggt tctttttgca
     1441 tgaaccagcc tggtttcgag tttcgtaacc ggcatttccg ttagcgtatg gaagtcatta
     1501 tccgggctga gccaatagac attatgcgaa accggatttc cctgtttgtc ggtcaggttc
     1561 agaacaacaa acgaaatgcc ctgggctttc tgaagaatat tgttcagcgt caatacctgt
     1621 tttacggatg tagtgtcgct attaagtttt ccagattgtt taaagaaggg tttaccatcg
     1681 aggccaaata tttcgacatg ccagccaaga cccggcctcg gaatatagct gcggttcacc
     1741 accgcaacgg atgaatcgtc atagttcagc tggatatgaa ccggttcgca ggcgttctgc
     1801 atgaaataat aaccggcgtt tggttccagg taccagtcaa agatctgcca gaccacactg
     1861 ggaaaagcgg cattgagctt ccacagcatc accccgccgg tttcatttag cttatggccg
     1921 gccgcttcaa agatcgcccg gtatccggag gcattcatca attgcatttt aacagaaaaa
     1981 tctttaaggc tttccggctt gccgaaccgg ctttccatag ctttaaaata aatatcgtat
     2041 ttgccatttc cagaacaggc atcatgatat ccccaggaat tattcagcgg ataaggcagg
     2101 tccttgtccg gaaccagatc cgggattatt ttaatgagcg aattataggg cggctgggaa
     2161 gggaggccgg tttcatcctt gaagacccag tccctgcctc tgtccgccag tttaaaatat
     2221 tgttgttctc ccacccagga ataggggccg ccgctgtaga caccggcggg cttatcgtcg
     2281 ggccaggatc ctttccattc tttcggagcc ttggagaacc cggatgaaca gggaatgaat
     2341 ggtcttgtac catccagcgt ggccacattg tcgcgcatgg cattatacaa ttcttcccgg
     2401 gcatgccctt cattgccgcc ggtccatacc agcaggctgg gatgattccg gatgcggtag
     2461 atggtgctca ccacgttgtc tacaaataca tgcgtttcca tcggccagtc cgccgatccc
     2521 ttaaatccgc cgtgcgtgtc tccggtgatc cagaagtcct gccacaccaa taatccatac
     2581 cggtcggtca ggttatagaa atcatccgtt tcccccaggc cgcctcccca gatccttacg
     2641 aggttcacat tggcattgcg gcagagatgc agttcataat cgtaccgcac cgaatcccgg
     2701 ttcagcatca tatccggaac ccaggcccca cctaccaggt gaaccctttt tccattcacg
     2761 tagaagtccc ggcgcaccca gccgttcacc atttccgttt tggaggaaac cgtgcgaatg
     2821 ccgaacagaa aagaagtatc gtccgaaatc ccggcgggct ggacaaactg tatccggatg
     2881 cggtacaagt tcggttgtcc atatccgtta ggccaccaga gggttggccg gtggatggtc
     2941 agcgcacgga catcctccgg acccagcgca atcccgaggc tgccgttccc gtcaacgctg
     3001 gccggccggg tgaaactcag ggcctgtcct ttgaaatttt ccggcgtaat ggttatttta
     3061 aggttgccct cctgccgggt atgactgtcg ttagccagcg tgagatgcaa atggattcgg
     3121 gcgacgctgg tatcgggcaa tgcggggaga tccgttgtga tttgaggatg ggaaatgacg
     3181 gtatgcccgc tggtccggag gtaaaccggc aaccagatgc ccatattcct gtcccttacc
     3241 gccggtatcc agtcccaacc cacggaactc agcatggtta cgtttttacc gatatccccg
     3301 gtgggtccgc cattagcata aaaatccccc agcgcgttta attgcgggtg ggcgggcagc
     3361 ccgggataat ccaggggata aatcttaacc gccagcccgt tttcttttcc cggccttatt
     3421 tcactactta tattcaggct gtattcggca aacatcccta ccatcaaaga agagtctgcg
     3481 atgcgctttc cattcaccca tacttccgcc cggtaattga tccccttaaa gatcaactga
     3541 aaccgtttac ctgcatccga tgccggcaca gaaaagacgg tccggtacca gtacggtttt
     3601 ttccaggggt tgggatcgtt gggaaggtag ctgtactgtt ccaggtgata ttgatgattg
     3661 aaggaatcgg aggcatccgg gatcagcata ttgttcatgc cggaataggg atccggataa
     3721 attttatttt gcaccagacc ggtaagtacc gtggatggaa cggtaacggg aaaccaatag
     3781 tccggagatg ggtagccggg taatgaaagg gtatcaccac ccgtaaacac taccgctgag
     3841 gactggagaa aaaaatggct gagttttacc tgggtcaccg gtctgttttg tgcagacagc
     3901 gccaccggat acaaaaaaaa gataaaggaa aacgaaagaa aagatttcat tacggtcata
     3961 ttatcgggta gacgggagca catttacttc agcacagaat tgctgataaa agcaatccgc
     4021 ttgctgtctg gcgaccagga tggagtattg atgctaccct gcccgccata aaaataggca
     4081 atcacttttg ccgggccgcc gccaaagggc atcagccgca gatagacatg cttataggga
     4141 ggatgaatgc ccggttgtac ttcctctttc aaaaaggaaa tgaaaacgat ccatttgccg
     4201 tccggcgaga tatgcgcaaa ccaattgtta aatccatcgt cagtgacctg ttcgggatcc
     4261 gtgccgtccg gcttcatccg ccagatctgc atggagccgg accttaccga attaaaatag
     4321 atatactttc catccgggct gtattccgga ccgtcatcca gtcctttggt ttgcgtgagc
     4381 ctgatttctt ttccgccttc ggccggaatt ttatacacat caaattctcc gttccgttca
     4441 ccgcagaaaa cgaggaattt tccatcaggc gaccatccat gcagatagga aggccccttc
     4501 ggggtaattt gtttcggtga cccgccgtca atgggcacgg tataaatgat ggagcctccc
     4561 agcgcccgca cactactact tagccccagc atctttccat caaaggagat cacgtggtcg
     4621 ttgttgttat tctttatgtt gccggtattt accgg
//
LOCUS       DASYCU010000094         4771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3070874, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000094 DASYCU010000000
VERSION     DASYCU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070874"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VGM24_01840"
     CDS             complement(<1..628)
                     /locus_tag="VGM24_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEY4154129.1"
                     /translation="MKIPFVDLKAQYESIKPEIDGAIAAVIGQTAFIGGYFVTRFEEE
                     FAKTYGVKHVLGCANGTDSLYILMKMMGIGKGDEVITSANSWISSSETVTQTGARPVF
                     VDVHPEYYSLDETKLEEKITTRTRVVIAVHLQGQLCDIEEIRRICDKHKLFLIEDCAQ
                     SHFSEFKGKRAGLFGHAGSFSFYPGKNLGAYGDAGCIVTNDDALADKCR"
     gene            complement(625..1674)
                     /locus_tag="VGM24_01845"
     CDS             complement(625..1674)
                     /locus_tag="VGM24_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154130.1"
                     /translation="MNRLLFRLSTIVLPVGALICLFNYFVDPANIFTSKKYVAGIAAI
                     LSQGHNVDQVSNYNERLLQEQMVRRLSKTPDIIILGSSRIMEIGSDFFPGKKVLNCGV
                     SHANVNDLVALTGLLDSLGRLPAELYINLDPDLISEKGTSEWQSLSAYYDRFINRNSP
                     AGTATEETGGSNEAHKIYSLVSFAYFKESVNFLIKGSTKKYYDVGSDQPPLYGRFSDG
                     SICYPASYTHPDTLKIASDALRIGSSQGLFQPDSARMELLGSLIDFLNKKNVRIHFIM
                     LPFHPAFYQGVNNHQPGLFNKYEKYFRNLASQKRVSIKGDFDATALHIPGSSFYDMYH
                     CSKAAIQSLSLTDQP"
     gene            complement(1671..3137)
                     /locus_tag="VGM24_01850"
     CDS             complement(1671..3137)
                     /locus_tag="VGM24_01850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY4154131.1"
                     /translation="MLFNSFEFAIFFPVVTILFFLLPHRLRWLLLLLASCFFYMFFKP
                     VYIFILLFTIVIDYFAGRWIAAEHVGRKKKQLLLLSIFANIGVLVVFKYYNFIAENLN
                     IVLRSVKENGQIPILNILLPIGLSFHTFQAMSYTIEVYRGNQKPERHFGIYALYVMFY
                     PQLVAGPIERPQNMIHQFHEVKKFNYENVRSGLILIAWGLFKKVVIADRLALFVNQVY
                     GNPFNYTGLPLIIGSVFFAFQILCDFSGYSDIAIGSAKCMGYHLMTNFDRPYMATTIG
                     EFWRRWHISLSTWFRDYVYIPLGGNRVSRGRWYFNILIVFLLSGIWHGANWTFAVWGL
                     LHGFYLLGETSFYRRFQKLREASGVLATLIKRTVVFILVLIAWVFFRSKDIAQAFYII
                     RHFPQHIPEQLKLVLSNEHFSRLSLLYLNQPGSEFIFAVLFILILILIHIVQKKQKFE
                     QWLIRKPVYARWSFYYGMVLAFIYFGVFNRTEFIYFQF"
     gene            complement(3144..3617)
                     /locus_tag="VGM24_01855"
     CDS             complement(3144..3617)
                     /locus_tag="VGM24_01855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4154132.1"
                     /translation="MNKPGIVKAGITEVEFGLNVKIVEPVNLYGCRIGENCFIGPFTE
                     IQKGVVIGEKTKIQSHSFICEWVTIGHHCFIGHGVMFINDPFTSGGPAAGDKNKWKAT
                     RIGNYVSIGSNATLLPVSICDQVVIGAGSVVTKDIVEPGVYMGNPAKKTTRSKGE"
     gene            complement(3610..4635)
                     /locus_tag="VGM24_01860"
     CDS             complement(3610..4635)
                     /locus_tag="VGM24_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154133.1"
                     /translation="MNTKYKFAIIGCGRIAQRHAEHISRLGKLIAVCDQVPEKAKALA
                     EKYQAKEFTALEEMLLACPDIDVVAVCTPNGLHAEHSIRSLKAGHHVLCEKPMALTVS
                     DCSAMINAAERANKRLFIVKQNRFNPPVEAVKKLIDQDKLGKIYSVQLNCFWNRNEDY
                     YKNSWKGTKNLDGGTLFTQFSHFIDLLYWFVGDVESVSALTGNFHHQGLIEFEDTGMA
                     VLKFINGAIGTVNYTVNAYRKNMEGSLTIFAGKGTVKIGGQYLNELEYQSLEGDPVNN
                     LPPGNPPNNYGQYFGSMSNHDKVYENLIDVLNGKGSIATSGMEGLKTVEIIDRIYRQA
                     KKNNTSE"
BASE COUNT         1278 a         1193 c         1046 g         1254 t
ORIGIN      
        1 tcctgcattt atccgcaagg gcatcgtcat tggtaacgat gcatccggca tctccatagg
       61 cgcccagatt tttcccggga taaaaactga acgaaccggc gtgaccaaac aggcccgccc
      121 gttttccttt gaactcggaa aaatgagatt gggcgcaatc ttcaatcagg aaaagtttgt
      181 gtttgtcgca gattcgccgg atctcttcta tatcacacag ctgaccctgc aggtgcacgg
      241 caatgaccac ccgggtgcgc gtagtgatct tttcttccag tttggtttcg tccaggctgt
      301 agtattccgg gtgaacgtcc acaaaaacag gcctggcgcc cgtctgcgta acggtctcgg
      361 aactggagat ccagctgttg gcagaggtga tcacctcatc ccctttgccg atgcccatca
      421 tcttcattaa tatatacagt gagtccgtgc cgttggcgca gcctaacacg tgcttaaccc
      481 cgtaggtttt cgcgaactct tcctcaaaac gggttacaaa gtagcctcca ataaaagccg
      541 tctgcccaat cactgctgcg atcgctccgt caatttccgg tttgatggat tcatactggg
      601 ctttcaggtc gacaaaagga attttcatgg ctgatcagtt aatgaaaggg attgaatcgc
      661 cgccttcgag caatgataca tgtcataaaa tgatgacccg ggtatgtgca atgccgtcgc
      721 gtcaaaatcc cccttaatgg aaacccgctt ttgggaagcc agattccgga agtatttctc
      781 atatttatta aacagacccg gctgatgatt attcactccc tggtaaaatg ccggatgaaa
      841 aggcagcata atgaaatgaa tccggacatt tttcttattc agaaaatcga tcagcgagcc
      901 cagcaattcc atccgggcag aatcgggttg gaataaaccc tgactgcttc cgatgcgtaa
      961 agcatccgaa gctattttaa gcgtgtccgg atgggtatag ctggcgggat aacaaataga
     1021 tccatccgaa aaacgtccat acagtggcgg ctggtcggag cctacgtcat aatacttctt
     1081 cgtacttcct ttaatcagaa agttcaccga ttctttgaag taagcaaagg agaccagcga
     1141 ataaatttta tgggcttcat tcgacccccc tgtttcttcc gtcgcggtcc ccgcgggact
     1201 attccggtta ataaaacggt cataataggc agaaaggctc tgccattcgg atgtcccctt
     1261 ttcagagatc agatcgggat ccaggttaat ataaagctcc gccggcagac gtcctaaact
     1321 atctaacagg ccggtcagcg cgaccaggtc attaacattc gcatgcgata ccccgcaatt
     1381 caggactttt ttcccgggaa aaaaatccga cccgatttcc atgatacggc tggaacccag
     1441 aatgatgatg tccggcgttt tagaaagtct cctcaccatc tgttcctgga gcaatcgctc
     1501 attgtagttt gaaacctggt ccacattatg accctgggag aggatggcgg caattccggc
     1561 cacgtatttc ttggaagtaa agatattggc gggatcaaca aaataattga ataaacaaat
     1621 cagcgccccg accgggagca caatggttga aagcctaaac aggagccggt tcaaaactga
     1681 aagtaaataa attctgtacg gttaaatact ccaaaataaa taaatgccaa aaccattccg
     1741 taataaaacg accatctggc ataaaccggt ttcctaatca gccactgctc aaatttttgt
     1801 ttcttttgca caatatggat caggatcagg atcaggataa aaagaacggc aaatatgaat
     1861 tcgcttcccg gctgattcag gtacaaaagc gacaaccggg agaaatgttc attgctgagg
     1921 accagcttca gctgttcggg gatatgctgt ggaaagtgcc ggatgatata aaatgcctgc
     1981 gcgatgtctt tggaacggaa aaagacccag gcgatcagga ccaatataaa aacaaccgtt
     2041 ctttttatca gagtggccag cacgccggaa gcctcccgga gcttttgaaa ccggcgatag
     2101 aagctggttt cccctaaaag gtaaaagccg tgcaacaggc cccagacagc gaacgtccag
     2161 ttcgcgccgt gccagattcc gctgagtaaa aaaacaatca ggatattgaa ataccatctt
     2221 cccctggata cccggtttcc gcctagtgga atgtatacat aatccctgaa ccaggtggat
     2281 aaagaaatat gccagcggcg ccagaactcg ccgatggttg tggccatata aggccggtca
     2341 aaattcgtca ttaaatgata gcccatacac ttcgccgaac cgatggcaat gtctgaatat
     2401 ccggaaaaat cgcacaatat ctggaatgcg aagaacacac tcccaataat cagcggcagt
     2461 ccggtataat taaacggatt tccatatacc tgattcacaa aaagggccag ccgatccgcg
     2521 atcaccacct ttttaaaaag cccccaggca ataaggatca ggccactgcg tacattttcg
     2581 taattaaatt tttttacttc gtgaaactgg tgaatcatgt tttggggcct ttctatggga
     2641 ccggccacca attgcggata aaacatgacg tataaggcat aaatgccaaa atgcctttcc
     2701 ggtttctgat ttccccggta tacctcgatg gtataactca tcgcctgaaa agtatgaaag
     2761 gaaagtccga tcggaagcag gatgtttaat atagggatct gtccgttttc tttaaccgac
     2821 cgcaatacga tattaagatt ttccgcaatg aaattataat acttgaaaac gaccagcacc
     2881 ccgatattgg caaaaatgct gagcagcaac aattgctttt tctttctccc cacgtgctcc
     2941 gcggcaatcc atcttccggc aaaataatca atgacaatcg taaacaggag tatgaaaata
     3001 tacaccggtt taaaaaacat atagaagaaa caactcgcca gtaacagtaa cagccagcgg
     3061 agccggtgcg gcagcagaaa aaacagaatc gttacgaccg gaaagaaaat ggcaaattca
     3121 aaggaattaa aaagcatgct tctctattct cctttggacc gggtggtctt ttttgccgga
     3181 tttcccatat agacaccggg ctccacaatg tcttttgtga ccacggaccc ggcgccaatc
     3241 accacctggt cacaaatgct gactggaagc agggtggcgt tggatccgat agaaacataa
     3301 ttgccgatcc gggtcgcctt ccatttattt ttatcgcccg ccgccggacc tccggatgta
     3361 aaagggtcat tgataaacat taccccatgg ccaataaaac agtggtgacc gatggttacc
     3421 cattcgcaga taaaactgtg ggactggatt ttcgtctttt ccccaatcac cactcctttc
     3481 tggatttcgg taaacggccc gatgaaacaa ttttctccga tccggcagcc atacaggttc
     3541 accggttcga ctatttttac gttaagcccg aattccacct ctgtaattcc cgcttttacg
     3601 atgcctggtt tattcactgg tattattttt tttcgcctgg cgatagatcc tgtcgatgat
     3661 ctctacggtt ttcaaccctt ccattccgct ggtggcaatg cttcccttcc cgtttaacac
     3721 atcaatgagg ttttcgtata ccttgtcgtg gttggacatg gatccgaaat attgcccgta
     3781 attgtttggc ggattccccg gaggaaggtt gttcaccgga tcaccttcca ggctttgata
     3841 ttccagttca ttgagatatt ggccaccgat cttcaccgtt cccttcccgg caaagatcgt
     3901 gagagagccc tccatatttt tcctataggc attcaccgta tagttaaccg tgccgatcgc
     3961 gccgttgata aacttcagga ccgccattcc ggtatcttca aactctatca acccctgatg
     4021 atgaaaattt ccggtcaggg ccgaaacaga ttccacatct cccacaaacc aatagagcag
     4081 gtcaataaag tggctgaact gggtgaataa agttccaccg tccaggtttt ttgtcccttt
     4141 ccaggaattt ttataatagt cttcgttccg gttccagaag caattcaact gcacactata
     4201 gattttcccc agcttatcct ggtctatcag cttttttact gcttcaaccg gtgggttaaa
     4261 gcggttttgc ttaacgataa acaaccgttt attggcccgt tccgccgcat tgatcatggc
     4321 agaacaatcg gaaacggtca gggccatcgg tttttcgcat aatacatgat gacctgcttt
     4381 cagggacctg atgctgtgtt ccgcatgcag cccattaggc gtgcagacgg caaccacgtc
     4441 aatatccggg caggccagca gcatctcttc cagggcggta aattcctttg cctggtattt
     4501 ttcggccaat gcctttgctt tttccggaac ctggtcacat accgcgatga gttttcccag
     4561 cctgctgatg tgctccgcgt gcctttgggc aatgcgcccg caaccgatga tagcgaattt
     4621 atattttgta ttcatttccc aattcctctc ttaagtgatc ttctgatcgg ttctgttctg
     4681 atcagcatgt tcatatatat ttccttggat ttgcggtccg atttaaacgc ctggtgtttt
     4741 tgcaccggca ggtgcatgat tttttcaaat t
//
LOCUS       DASYCU010000095         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3505313, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000095 DASYCU010000000
VERSION     DASYCU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505313"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VGM24_01865"
     CDS             complement(<1..1464)
                     /locus_tag="VGM24_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY4154134.1"
                     /translation="MFNNLRNSILILTTGLGLAACHGHSTGNKKTFRYNEPTGIATLD
                     PAFAKNQSIIWPVHQLYNTLVETDSQLHIVPSLARSWEISANKLVYRFHLRTDVFFHD
                     NDAFPGGKGRKMLASDIVYSLSRIVDRKTASSGAWIFNERIAGPQAFRAPDDSTFELT
                     LLTPFHPMLGLLSMQYCSVVPREVVEKYGKDFRSHPCGTGPFRFKSWEEGQDLVLLKN
                     GHYFEKDAAGRSLPYLDAIKISFFDNRATEFLLLQQGRLDFMNDIDPSFKDEVLNKKG
                     ELKKEWVGRLQLSKSPYLNTEYLGIVVDSSLPMIRHSPLRLLKVRQAINYGFDRRKMM
                     MYLRNSIGTPAESGFIPPGLPCFDSTLVKGYHYDPARARRLLKEAGFPDGQGLPLITL
                     KTIDTYADLAGFIVRELQDIGIRVQVDIVQKSLLLEETAKSEAPFFRGSWIGDYPDAE
                     NYLSVFYSENPAPPNYTRYHNPQFDDLYRKSLLENNDS"
BASE COUNT          406 a          451 c          444 g          391 t
ORIGIN      
        1 gctgtcatta ttttccagca gtgatttccg gtacagatca tcaaactgcg gattatggta
       61 ccgcgtataa ttgggcggag ccggattttc agaataaaaa acgctcaggt aattctccgc
      121 atcgggatag tcgccgatcc agctgccccg gaaaaaagga gcttccgatt tggccgtttc
      181 ttccagcaac aggcttttct gtacgatatc cacctgcacg cggatcccga tatcctgcag
      241 ttcccgtaca ataaacccgg cgaggtcggc ataggtatct atcgttttca gcgtgatcag
      301 cggcaaaccc tgaccatccg ggaatccagc ctccttcagc aagcggcggg ccctggccgg
      361 atcatagtga taccctttca ccagcgtgga atcgaaacag ggcaggcccg gcggaataaa
      421 accgctctcc gcgggagtgc cgatggagtt gcggaggtac atcatcattt tcctccggtc
      481 aaatccgtag ttgatcgcct ggcgcacttt aagcaggcgt aacggcgaat gcctgatcat
      541 gggaaggctg ctgtcgacca caatacccag gtattccgta ttgaggtaag gacttttcga
      601 aagctgcaac cggcctaccc actctttttt cagctctcct ttcttgttca gcacttcatc
      661 cttgaaagac ggatcgatat cgttcatgaa atccagccgg ccctgctgca gcaataaaaa
      721 ttccgtagcg cggttatcaa agaaactgat cttgatggcg tccaggtatg gcagggaccg
      781 gcccgcggcg tccttctcaa aataatgccc gttttttagc aggaccaggt cctgcccttc
      841 ctcccagctt ttaaaccgga agggaccggt tccgcaggga tggctccgga aatccttacc
      901 gtatttctcc accacttccc ggggaaccac cgagcaatac tgcatgctga gcaatccgag
      961 catgggatga aagggcgtga ggagcgtcag ttcgaacgtg gaatcgtccg gcgcgcggaa
     1021 cgcctgcggg cccgcaatcc gctcattaaa gatccaggcg ccgctgctcg ctgttttccg
     1081 gtccacgatc cgggacaggc tgtacacgat gtccgaagcc agcatcttcc ggcctttgcc
     1141 gccgggaaaa gcatcgttgt cgtgaaaaaa aacatcggtg cgcaaatgaa aacggtagac
     1201 cagcttgttt gccgagatct cccaggaacg cgccagggaa ggaacgatat ggagttggga
     1261 atcggtttcc accagggtat tgtacaactg gtgaaccggc cagataatgg attgattctt
     1321 tgcaaatgcc ggatccaggg tcgcgatccc cgtcggctca ttataccgga atgttttttt
     1381 atttccggtg gaatggccgt ggcaggcggc cagcccgagc ccggttgtta aaatcaggat
     1441 gctgttccgc agattattga acaaatggtt tattttcatt gaatgcggcg aacggtaaag
     1501 attaaaagta atcaggaatc attaaacgcc tttagacttc aaggaaaaaa caaaattaac
     1561 ggatgaaaag aacagccgtt ttatggatgc tgacctgcgg ggctttttgc ttttccaaag
     1621 cgcaggaccg gatggcgcct ccgtcattca ctcaccagga tacactccgg ggctccctta
     1681 atccggagcg gg
//
LOCUS       DASYCU010000096         5865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_460513, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000096 DASYCU010000000
VERSION     DASYCU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460513"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..441)
                     /locus_tag="VGM24_01870"
     CDS             complement(223..441)
                     /locus_tag="VGM24_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154135.1"
                     /translation="MKVKLPGVRIPPSPQKRILAPAAKLRVFCLEQTRELVRSGCEEQ
                     KTKAANGRLKVQKWDFNPPQKIAEGKLL"
     gene            complement(400..484)
                     /locus_tag="VGM24_01875"
     tRNA            complement(400..484)
                     /locus_tag="VGM24_01875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(448..450),aa:Ser,seq:tga)
     gene            703..1263
                     /locus_tag="VGM24_01880"
     CDS             703..1263
                     /locus_tag="VGM24_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY4154136.1"
                     /translation="MPACAEGNKSAPDFKCLFDIYKNRVFRYAQAISHSPFIAEEITQ
                     EIFIKLWLYRDTLSRVDNLDAYIFRMARNKTLNYLRRAAHETKILKELQSRMIPENNN
                     AAEHLLAADQHKMLQEAITRLSPQRRLVYQLSRNQGLNHKEIASQLRLSPNTVKNHLV
                     NALKFIREYLNVLNSVIVFLLLFAGL"
     gene            1353..2375
                     /locus_tag="VGM24_01885"
     CDS             1353..2375
                     /locus_tag="VGM24_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY4154137.1"
                     /translation="MDRSSRLNYLFEKYLHRLSSAEEVEELVRLLQLDQPPGALRDRL
                     SDLWESVKEEPVPDTVDWENMYQTVIHSEERLPVIRERKNRTWLKIAAALFILVSGSV
                     LYRNLNSRVAGTVKPVTGTTVRPANIARSNKKQTIHLPDGSTVILNTDSHLDYPSAFT
                     GKTREIYLTGEAYFDIKHDSKKPFLVHTGSIVTRVLGTAFDIKAYPGDESIRVTVTRG
                     KVQVLNKNKSLGLIGANQQISFSRKSETVMQKVVDPKPLIAWKPEEIAFNDIAMLDAA
                     KEIERRFGTKVEFANPLIKNCRVTATFSEDDMLDEILAVICGVTRSQYIIQNNKIIIN
                     GKGCNE"
     gene            2458..>5865
                     /locus_tag="VGM24_01890"
     CDS             2458..>5865
                     /locus_tag="VGM24_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154138.1"
                     /translation="MKFLFPVMGRMHDSLSAVNPKYKSRIMKISCLLIAVGCSGLQLL
                     MANSGKAQGLNDVKVTLELNNEPLKAAFAKIEKQTDFRFAYNKRQVDQYERISVEKDD
                     YPLQQLLNILLDKTDLSFRLIRNKIIIFQKDKAETAPAAVESSPTADGGIKGRITDEK
                     GQAVAAASVVIVGQKNKGTAADPQGEFTITGIQPGKYTLRISGVGFETLNQEVTIADN
                     QVQELTFQLKSNNNSLDQVIVTGYSRQSKRDVTGAASTVSADIISQTPVTDVTTALQG
                     RVAGVSVDDQGGPGNSGVIRIRGVGSLGNNDPLYVIDGVQVRIGGGTGSQNIANLLNP
                     NDIESVTVLKDPSLTALYGSEGSNGVIVITTKSGKKGEPQLEYSSYVGEQLPNKFPSL
                     ISPQQQADALYKSYSNSGLGFPYGSFYGSGATPVLPDYIIEGSTPNVGVSANDPAADP
                     SLYNFLNYRILKANKSGTDWWKELFKPAFVQNHQLAISGASDKSNYAITLGYLNDNGT
                     LLNSYFKRLSLRVNTDFKVKPWLRVGENVEFSYTTNNTISTSNEANNQNDFNNNITDL
                     YSLSPLLPTHDIKGNIAGTNGAVILGGSDPLISRSSANNSKNYTESVIGSAYIEIEPI
                     KNLTFQSKIGAQFVPNQYHAFSDSFPQEPIPSHLTYYYEGGSTYTDWRWTNKIAYSMT
                     FRDIHKISVFAAYEARELRSRYNTIVMTNLISGLPNFQYVSDGVLNPNFPPTGSGSVQ
                     TSTSVFGNLTYSLMDKYLFSGTLRNDGSSIFGLQHQQGIFPAVSAGWRISQEGFMDKM
                     TWINDLKLRGSWGRAGNDAIPPGQQFGLIRSNDPIYGGYDLEGTNISQILGAYASQNG
                     NQRIHWETNETTNIGLDAAFLNNRLTLGFNWFDRNTKDLLYEPPYTGTAGAPSAPYKN
                     IMSFTNKGIEIELGFRGGNSNKFSYDMNFNISGYRSNVDYIDGDSATFIDLAIFAPTH
                     YNLTRNMVGHPVSSFFGFVQTGIFQNMDDVQNSPTQAGISKTDPSGIGHFKFKDITGP
                     NGKPDGNVGPEDQTFIGNPNPKFSFGYNLNLNYDHFDLGLFIQGVSGNKIFNYWRSFS
                     EWPGSLRSGSLDTWTTDNPTAKLPIYTQDNINGNNDNVPSSFFVEDGS"
BASE COUNT         1648 a         1446 c         1367 g         1404 t
ORIGIN      
        1 atatgttcat atcaaaactt aggaaaaaac tggaatttga tccgaatatc aaaattgccg
       61 ttatacgcgg taaaggatat aagctggaga ttaacgctta agaagaatct cctcgccaaa
      121 acggatctca tcagacttca atccagcatt tgtaaaattc tatcgtaaac tgctgagatg
      181 gtcagcggga atcgaacttt ttggttttat taacaatctc atttacaata acttgccttc
      241 ggcgatcttt tggggagggt taaagtccca tttttgcacc ttcaggcggc cgttggccgc
      301 cttcgttttt tgttcttcgc atccgctgcg aacaagctcg cgcgtctgct ccaaacaaaa
      361 aacccgcagc ttcgctgcgg gtgcaagaat gcgcttttgc ggagagggag ggattcgaac
      421 ccccggaagt ttgaccttca acggttttca agaccgccgc aatagaccac tctgccacct
      481 ctccggtcgc aaaattaggg tttgagggga aatcacaaaa tataaatgac cagcttgcgg
      541 ccgggctttt ccgtcataaa ataaattcat tctgcaagag ccttccctgc cgggttccgg
      601 gttcaaccag cagtcgaaat ccggccgttc atttaattga accggctaat tgataattta
      661 ttacttttac gccactgatt gcttaggtta aaaacgacgg ctatgcctgc ttgtgctgaa
      721 ggaaataagt ctgcccccga ttttaaatgt ctttttgaca tctataaaaa ccgggtattc
      781 cggtatgccc aggccatctc ccactcccct tttattgccg aagaaatcac gcaggagata
      841 ttcatcaagc tctggcttta ccgggatacg ctgagccggg tagacaacct ggatgcctat
      901 attttccgta tggcaaggaa caaaaccctc aactacctcc ggcgggcggc acatgaaacg
      961 aaaattctga aggagcttca aagccgaatg atcccggaaa acaataatgc agcggagcac
     1021 ctgctggccg ccgaccagca taaaatgctc caggaagcaa taacccggct ctccccccag
     1081 cgccgtctgg tttaccagct gagccggaac cagggtttaa atcataagga aatcgccagc
     1141 cagctccggc tttcccccaa tacggttaag aatcacctgg tcaatgccct caaatttatc
     1201 cgggaatacc tgaacgtatt gaattcagtc atcgtttttc tactgctgtt tgccgggcta
     1261 taaaaaaatc tttccctgca ttagtcctat ttcttttccc aggcgtcttt atttgtaacc
     1321 cagcccgaaa accggttatt caaccccctc atatggatcg atcgtccagg ctgaactatc
     1381 tttttgaaaa gtatctccat cgtctttcca gtgcggagga agtggaggaa cttgtccgct
     1441 tattgcagct ggaccagcct ccgggtgccc tcagggaccg gctaagtgat ctttgggaaa
     1501 gtgtaaagga agagcctgtt cccgatacgg tggattggga aaacatgtat caaacggtca
     1561 ttcattcgga agaaagactg ccggtgatcc gggaaagaaa aaaccggacc tggctgaaga
     1621 tcgctgccgc tttgtttata ctggtttccg ggtcggttct ttaccggaat ttgaatagcc
     1681 gggtggccgg taccgtaaag ccggtcaccg gtacaacggt aagaccggcg aatatcgcca
     1741 gatccaataa gaaacaaacc atccatctgc ccgatggaag tacggtaatc ctgaatacgg
     1801 acagccacct ggactatcct tctgccttta cgggaaaaac cagggaaatc tatctgacgg
     1861 gtgaagccta ttttgatatc aagcatgatt caaaaaagcc attcctggtt cataccggat
     1921 ccatcgtgac ccgggtactg ggaacggcct ttgacattaa agcgtatccc ggggacgaat
     1981 ccatacgggt taccgtgacc cgcggaaagg tacaggtcct gaacaaaaat aagagcctgg
     2041 gtctgatcgg tgcgaatcag cagatcagct tcagcaggaa atcagaaacc gtaatgcaaa
     2101 aagtagtgga ccccaaaccc ctgattgcct ggaaaccgga agagatcgct tttaatgata
     2161 tcgcgatgct cgatgcggca aaagagatcg aacggcgttt cgggacaaag gtcgaattcg
     2221 ccaatccgct cattaaaaac tgccgcgtga cggcaacttt ttccgaagat gacatgctgg
     2281 acgagatcct ggcggttatc tgcggagtaa ccagatcgca atatattatt caaaacaata
     2341 aaataatcat caatgggaaa ggttgtaacg aataaataaa aatacacccc gggtggtgcc
     2401 gggatgtagg gtcattttaa acaattagag cgtaatcatt taaaacatat caaaactatg
     2461 aaatttttat ttcctgttat gggaaggatg cacgattccc tttctgcagt caacccaaaa
     2521 tataaatccc ggatcatgaa aatcagttgt cttcttattg ctgtcggctg cagcggattg
     2581 cagttgttaa tggccaattc cggtaaggcc cagggattga atgatgtgaa agtgaccctg
     2641 gaactcaata acgagccgct gaaagccgca ttcgcaaaga tcgaaaagca aaccgatttc
     2701 cgttttgctt acaataaaag acaggtggac cagtacgaac ggatctcggt ggagaaggat
     2761 gattatcccc ttcagcagct gcttaatatt ttattggaca agaccgatct ctccttccgg
     2821 ttgatccgga acaagatcat aatatttcaa aaggataaag cggagaccgc tcccgctgct
     2881 gtggaaagca gtcccacggc agatggcggc atcaaaggca ggataaccga tgaaaagggc
     2941 caggcggtag ctgccgcttc cgtggtgatc gtcggacaga aaaacaaagg cacggcagca
     3001 gatccccagg gggaatttac gataaccggt atccagcccg gaaaatatac cctccggatc
     3061 tcgggtgttg gtttcgaaac ccttaaccag gaagttacga tagcggataa ccaggttcag
     3121 gagttgacct tccagttgaa atcgaataac aattcgcttg atcaggtgat cgtcaccggt
     3181 tattcccgcc agtccaaaag agatgtgacc ggcgccgcgt caacggtttc ggcagatatt
     3241 atttcccaga caccggttac tgatgttacg accgcattgc agggaagagt ggccggggtc
     3301 agcgtggacg accagggcgg tccgggcaat tcaggcgtta tccggatccg gggagtcgga
     3361 tccctgggca ataacgatcc cctttatgtg atcgatggcg tacaggtgag aattggcggg
     3421 ggaacgggca gccagaatat tgccaacctg cttaatccaa acgatattga aagcgtaacg
     3481 gttttaaagg acccttccct aaccgcctta tatggttccg aaggatcaaa cggagtgatt
     3541 gtcataacca ccaagtcggg aaagaaagga gaaccgcaac tggaatattc ttcctatgtg
     3601 ggagaacagt tgccgaataa attcccctct ttaatttcac cccagcagca ggcagatgct
     3661 ttatataaat cctacagcaa ttccggcctg ggttttccat acggcagttt ttacggatcc
     3721 ggtgccaccc cggtattgcc ggattatatc attgaaggtt ccacccccaa cgtcggcgta
     3781 tccgcaaatg atccggctgc cgatccgtcc ctgtacaatt ttttaaatta ccggatatta
     3841 aaagccaata aatcaggaac cgactggtgg aaggaactgt tcaaaccggc attcgtccaa
     3901 aatcaccagc tggccatcag cggagcatcc gacaaaagca attatgccat caccctgggc
     3961 tacctgaatg ataacggaac gctgctgaat tcgtatttca aaagattgtc attgcgggta
     4021 aatacagact ttaaagtaaa accctggtta agggtcgggg aaaatgtgga attctcctac
     4081 accacgaaca atacgatctc caccagcaat gaagccaata accagaatga ttttaacaac
     4141 aatatcactg acctctattc tttgtcccct ttactgccca cacacgatat caagggaaat
     4201 attgccggaa ccaacggcgc cgttattctg ggcggaagcg atccgctgat cagcagatcg
     4261 agcgcaaaca attcaaaaaa ctatacggaa agcgtaatcg ggtccgcata tattgaaatc
     4321 gagcctataa aaaacctgac cttccaaagc aagatcggag cccagtttgt tccgaaccaa
     4381 taccatgcat tcagcgattc gtttccgcag gaacccatcc cgagccatct gacctattat
     4441 tatgaaggcg gttccacata caccgactgg cgctggacga ataaaattgc ctacagcatg
     4501 accttccggg acattcataa aatttctgtt tttgccgcct atgaagcccg cgaactgcga
     4561 tccaggtata ataccattgt gatgaccaac ctcatatccg gcttacccaa ctttcaatat
     4621 gtttccgatg gggtgctgaa tccgaatttc ccgcctaccg gatccggatc ggtgcaaacc
     4681 agtacctctg tttttggaaa cctgacttat tccctgatgg acaaatatct tttttccggt
     4741 accctgcgca atgacggatc ttccatattc gggcttcagc accagcaggg tattttccct
     4801 gcggtcagcg caggatggcg gatatcccag gaaggattca tggataaaat gacctggata
     4861 aacgatctga aattaagagg ctcctggggc cgcgccggta acgatgccat accgccggga
     4921 cagcagtttg gactcatccg gtcaaacgat ccgatttacg gaggatatga tctggaaggg
     4981 accaatatca gccagatcct cggcgcctat gcctctcaaa atggaaacca gcgaatacac
     5041 tgggaaacca atgaaacaac caatatcgga ctggatgccg cctttttaaa taaccggctc
     5101 acacttggat ttaactggtt cgatagaaat acaaaggatc ttctctatga accgccttat
     5161 accggaacgg cgggcgcacc ttcggcgccc tacaagaata taatgagttt caccaataag
     5221 gggatagaaa ttgaactggg tttccggggc gggaattcca ataaattcag ttatgatatg
     5281 aacttcaata tctccggcta caggagcaac gtggactaca ttgacgggga ctctgcaacc
     5341 tttatcgacc tggccatttt tgcccctacg cattataatc tgacgcgcaa catggtcggc
     5401 cacccggtat ccagtttctt cggttttgtt caaacgggga ttttccaaaa tatggatgat
     5461 gtccagaaca gtccgaccca ggccggaatc agcaagaccg atcccagtgg aatcgggcat
     5521 tttaaattta aggacataac gggccccaat ggtaaaccgg atggaaatgt agggccggaa
     5581 gaccagacct tcatcggaaa tccgaatcca aaattttctt tcggttacaa cctgaacctg
     5641 aattacgatc attttgacct gggcctgttc atacaggggg taagcggaaa taaaatattc
     5701 aactactggc gttcgttctc cgaatggccg ggctccctgc gatccggatc cctggatacc
     5761 tggacaacgg ataatcccac ggcgaaactt ccgatctata cccaggataa tatcaacggc
     5821 aataacgata atgtgccttc cagttttttt gtcgaagacg gcagt
//
LOCUS       DASYCU010000097         5411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4808993, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000097 DASYCU010000000
VERSION     DASYCU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4808993"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1221)
                     /locus_tag="VGM24_01895"
     CDS             complement(82..1221)
                     /locus_tag="VGM24_01895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4154139.1"
                     /translation="MKVVLISRATLYSVKGGDSVQVLQTAKQLGRLGISADVKLTNEP
                     INYSNYDLLHFFNITRPADILFHIRKAGKPFVISTILIDYSNYDQQYRKGLPGIFMRL
                     FPADNLEYVKCIARYLKGQDKLMTLSYLWMGQNRSIRELLLKASLLFSNSQLETTRIE
                     QQFRIKTNCIPVHNGVDTELFRYNDSLKKDKYLVLCAARIEGIKNQLNLIRALRGTEY
                     TLLLVGAPAPNHQSYYRECRKIASSNIRFIGFTDQAELVHYYQRAKVHVLPSWFEACG
                     LSSMEAAAMGCNIVITSKGYTREYYGEYAFYCDPGSPDSIFKAVNEAANAGFRNKLRE
                     KILSDYTWPKAASRIARAYHQILPADGHPEGNSKMREYFQSLQEF"
     gene            1398..2321
                     /locus_tag="VGM24_01900"
     CDS             1398..2321
                     /locus_tag="VGM24_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154140.1"
                     /translation="MAADLTDSILKVLAYFDLFDYPLRKEEIFAFLDQPVQPVVLSEA
                     LEELLERENIFLFQPFYSIRDNKELACRRVTGNLRAQPLLRIGNRISRILYQCPYVRA
                     IGISGSLSKNFAAEDADIDYFIITRSDRLWIARTMLHLIKKFSFLLGRQHWFCMNYFI
                     DEEALEIEEKNIFTAIELLTLLPLRGNSCLNEFYSGNTWTKEFCPNYRNRSAAADGPV
                     SDSRLKKILESLFDGRAGDWLDNYFMRLTSRRWKKKEGLQKRSVKGNLMSLQTGKHFS
                     KPNPGLFQKEILRLYQDNLKNRVPDRDRILL"
     gene            complement(2318..3133)
                     /locus_tag="VGM24_01905"
     CDS             complement(2318..3133)
                     /locus_tag="VGM24_01905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4154141.1"
                     /translation="MDINEQRVAAAFTKQSGIFDEYFSGNSIIRYKRKRVRDVIQEFI
                     PRPGAVLELNSGTGEDAVWLAEQGHRVHATDLSSGMLEVLRDKVALRGLQESVSSECC
                     SYTALHALKRKGPYDLIFSNFAGLNCTNELANVLDSFDRLLKPGGTVILVVMPKFCLW
                     ETALLFTGKFKTAFRRFFGKKGASARVEGNEFSCWYYNPSFIARTLTNSFQLARTEGL
                     CFLVPPSYIEQFPEKHPVIYRFLKKAEERLKTKWPWNRIGDYYIIALKKKTSG"
     gene            complement(3151..4611)
                     /locus_tag="VGM24_01910"
     CDS             complement(3151..4611)
                     /locus_tag="VGM24_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY4154142.1"
                     /translation="MNKILFSHSYFLRFDPKQWTIGQPYPPLGTLYGAAFVRAAGNEV
                     SLFDTQFSRHPSEIIPALDEFNPDFFVIYDDGFNYLTKMCLTNMREAAFGMIRLAKTR
                     GCISIVSSSDSTDQFKQYLDEGADFILLGEAEETLLELITAIQNGQAGFSQIKGLAFK
                     QEDKYIKTERRPVIRNLDALPMPAWDLIDLSPYRNTWISHAGYFSLNTATTRGCPFKC
                     NWCAKPIYGNRYNARSPRHVVKELKWLKSRYSFDHIWFCDDIFGLKPGWVNEFADLIE
                     AEKLKFKFKIQARADLLLEENNVSDLARAGCDNIWMGAESGSQKILDAMDKGITVEQI
                     KEATRLIRKYHMKPSFFIQFGYPGETKEDIEMTIRMINELLPYELGISVSYPLPGTVF
                     YEKVKHDLIQKSNWTDSDEMALMFRNTFQPAFYKQLHRFVHKSYRKHIAFENLRQLVK
                     HPAGGRFSQVLKALSGLYYVPAAYIDKRKLNQLEKN"
     gene            complement(4608..>5411)
                     /locus_tag="VGM24_01915"
     CDS             complement(4608..>5411)
                     /locus_tag="VGM24_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154143.1"
                     /translation="DNFGSGSVTDQSFSWRINNFFNHFFYSEQVLFLPGLAGYFFIKK
                     RNDLFLIWSICEFIGISLLGIFSQQHFKNLLAPLSLMTALSIERLLEIYSIPARQTMI
                     VVLICFFPKIWEPIGSFKNLFRSPEKTISPDSPSMNNQEDDYSKKLLGLWIRSNTDPG
                     SRVLIAGFAAIAQAYSERVSASIYFNVTQTQLAKNIFYQDIKRNAPGMICVPTFPTYF
                     QNVNADIRDSIDQLVSRKYILRDHRYGYNIFLKNEQPKWSFHKIINRMQ"
BASE COUNT         1355 a         1261 c         1328 g         1467 t
ORIGIN      
        1 aaaatgtatt tgacagctgg ctggatcccg ccagcaggac catcaagccg atggttgaaa
       61 tcgaatgagt ttcgccggtg ttcagaactc ctgtagggat tgaaaatatt cccgcatctt
      121 actatttccc tccggatgcc cgtcagcggg tagtatttgg tgatacgccc ttgctatacg
      181 ggatgcggct ttcggccagg tgtaatcaga gaggatcttt tcccggagtt tgttccggaa
      241 cccggcattt gcggcctcat tcacggcctt gaaaatagaa tccggcgagc cgggatcaca
      301 ataaaaagca tattccccgt agtattcccg ggtataccct ttggacgtga tcacgatatt
      361 gcaacccatt gccgccgctt ccatcgaaga aaggccgcag gcctcaaacc agcttggcag
      421 cacatgcact ttcgcccgtt gataataatg aaccaattca gcctgatcgg taaatccgat
      481 gaaccggata ttggaggaag ctattttgcg gcattcccgg tagtaggact gatgattggg
      541 tgccggggct ccgacgagca gtagcgtgta ctccgtgccc cgcagggccc tgatcaggtt
      601 tagctgattt ttaattcctt cgatgcgggc cgcgcaaagc accaggtact tgtctttttt
      661 cagggaatca ttataacgga ataattccgt gtccaccccg ttatgcaccg gaatgcaatt
      721 cgttttaatc cggaactgct gttctattct tgttgtttcc agctgggagt tcgaaaaaag
      781 cagggaagct ttcagcagca gctcccggat gcttctattc tgccccatcc acaaataaga
      841 aagggtcatc agtttatcct ggcctttcag ataacgggca atacatttca catattccag
      901 gttatcggcc gggaatagcc gcatgaagat cccggggagc cctttccggt attgctgatc
      961 gtaattgctg taatcaatta atatggttga gatcacaaat ggttttcctg ctttccggat
     1021 atgaaataga atatcggccg gccgggtaat gttaaaaaaa tgcagcaggt catagttcga
     1081 atagttgatc ggctcgttgg ttagtttcac atccgccgaa atgcccaggc ggccaagttg
     1141 tttcgcggtt tgaaggacct gtacgctgtc tcctcctttt acgctgtaca aagtcgccct
     1201 gctgatcaaa actactttca tatttatccg cctccggtat gatttgtaaa tcaggtgagt
     1261 aatggtgaga tgaggtctac actgaaaacg catcctgaaa tggaggggtt gcgtgaataa
     1321 atgatttccc gccgctattc ggaccggacg caacttggtg atcaaaatag ccgtttttac
     1381 tgctgaatac ctcttgcatg gcggcagatt tgacggatag catattaaaa gtgttggcct
     1441 attttgatct ttttgattac ccgctcagaa aagaggagat cttcgctttt ctggaccagc
     1501 ccgtgcagcc ggtagtcctt tcagaggcgt tggaggaact cctggagcgg gagaatatat
     1561 ttcttttcca gccgttttat tcgatccggg ataataagga gctcgcctgc cgccgggtga
     1621 ccggtaatct gagggcccag ccgctcctgc gtatcgggaa ccggatctcc cgcattcttt
     1681 accagtgtcc atatgtgcgt gcgatcggta tctccgggtc cctttccaag aattttgccg
     1741 ccgaggatgc ggatattgat tattttatta ttaccaggtc cgatagatta tggattgcca
     1801 gaacaatgct gcacctgatt aagaaattca gttttctgct gggccgccag cactggtttt
     1861 gcatgaacta ctttatagat gaagaggcgc ttgaaatcga agagaaaaat atctttaccg
     1921 caatagaact ccttaccctt ttgcccttgc ggggaaatag ctgcctgaat gaattctata
     1981 gcgggaatac ctggacgaag gagttttgtc cgaactaccg caaccggagc gccgcagcgg
     2041 atggaccggt cagtgactca agacttaaga aaatcctgga gtctttattt gatggccggg
     2101 cgggcgactg gctcgataat tactttatgc gcctaaccag ccgtcgctgg aagaaaaagg
     2161 aaggcctgca aaaaagatct gttaaaggga acctgatgag ccttcaaacc ggcaaacact
     2221 tctccaaacc caatccgggt ctttttcaaa aagagatcct ccgtttatac caggataatc
     2281 tgaaaaaccg ggttcctgac cgggaccgga tcctgcttta gcccgacgtc ttcttcttta
     2341 aggctatgat gtagtagtcg ccgatgcggt tccagggcca ttttgttttc agccgctctt
     2401 ctgctttttt gaggaaccgg tatatgacgg gatgtttttc cggaaattgt tctatatagg
     2461 aaggcggaac aagaaagcaa aggccttcag tccgggccaa ttgaaatgaa ttggttaatg
     2521 tcctggcaat aaaggatgga ttataatacc agcacgaaaa ttcatttccc tccacccggg
     2581 cggatgcgcc ttttttaccg aagaaacgcc tgaatgcggt tttgaatttt cccgtaaaga
     2641 gaagcgctgt ttcccagagg caaaatttgg gcatgaccac gaggatgacc gtgccgccgg
     2701 gttttaataa acggtcaaag ctgtcgagca cgttggcgag ttcgttcgtg caattcaatc
     2761 cggcaaaatt ggaaaagatc agatcatagg gcccttttct tttaagggca tgcagggcgg
     2821 tgtaggaaca gcactcggag ctgaccgatt cctgcaatcc acggagagct actttatccc
     2881 tgagcacttc cagcatgccg gacgaaagat cggttgcatg tacccggtgt ccctgttcgg
     2941 cgagccagac ggcatcctcg cccgtaccgc tgttgagttc cagtacggca cccggccggg
     3001 gaatgaattc ctgtataaca tcgcggaccc ttttccgttt gtaccggata atgctgttcc
     3061 cggaaaaata ttcatcaaat attccggatt gttttgtaaa cgccgcggcg acacgctgtt
     3121 catttatatc cattgggccg attggattga tcaatttttt tccagctggt ttaattttct
     3181 cttatcgata taggcagcgg gcacataata aagcccggac aatgctttta gcacctggct
     3241 gaaccttccg cctgccggat gcttcactaa ctggcgcaaa ttttcaaagg cgatgtgttt
     3301 ccggtaactt ttgtgaacga accggtgtaa ctgtttataa aacgccggct gaaaggtatt
     3361 tctaaacatc aatgccattt catcagaatc ggtccaattg gatttttgaa tcaggtcgtg
     3421 tttaaccttt tcatagaaaa cagttcccgg cagcggataa gaaacagaga tgcccaattc
     3481 atagggcagc aattcattga tcatgcggat ggtcatttca atatcttctt ttgtttcccc
     3541 cgggtagcca aactgaataa agaaggaagg cttcatgtgg tatttccgga tcagccgggt
     3601 ggcctctttt atctgttcca ccgtgatgcc cttatccatc gcatcgagta ttttttgcga
     3661 cccgctttcg gcacccatcc agatattgtc acatccggcc cgggccaggt cgctgacatt
     3721 attttcctcc aaaagcaaat ctgcccttgc ctgtattttg aatttgaatt tgagcttttc
     3781 ggcttcaatg aggtcggcaa attcgtttac ccatcccggc tttaagccga aaatgtcatc
     3841 acaaaaccag atatgatcaa aagaatagcg cgatttcagc catttcagtt ctttcaccac
     3901 atgccggggc gaccttgcgt tgtaccggtt tccatatata ggttttgcgc accagttgca
     3961 tttaaagggg catccccggg tagtggccgt gttcagtgaa aaatagcctg cgtggctgat
     4021 ccacgtattg cggtacgggc taaggtcgat caggtcccag gcgggcatgg gtaaggcatc
     4081 caggttcctg atcaccggcc ggcgctcggt ctttatgtat ttgtcctcct gcttaaaggc
     4141 aagtcctttg atctgggaaa aaccggcctg gccgttttga atggcggtta ttaactcaag
     4201 taatgtttct tcggcttctc cgagtaaaat aaaatctgct ccttcgtcga gatactgttt
     4261 aaactggtcg gtggagtccg agctggatac gatgctgata caacccctgg ttttagccag
     4321 ccggatcatt ccgaacgcgg cttcccgcat attggtcagg cacattttgg ttaaataatt
     4381 gaatccatcg tcatagatca cgaaaaaatc cgggttgaat tcatccaggg caggaataat
     4441 ttcggatgga tgcctcgaaa actgggtatc gaacagggaa acctcatttc ctgccgccct
     4501 cacgaaagca gcgccgtaca gggtgccgag gggagggtag ggctggccga tcgtccattg
     4561 ttttgggtcg aaccgtagaa aataggaatg cgaaaagaga atcttattca ttgcatccgg
     4621 ttaatgattt tatgaaaact ccatttgggc tgttcatttt tcaagaaaat attatatccg
     4681 taccggtggt ctctaagaat atatttccgg ctgacgagct gatcgatgga atcccggata
     4741 tccgcattca cattctggaa ataggttgga aaagtgggta cacagatcat tcccggggca
     4801 ttccgtttga tgtcctgata aaaaatgttt ttagccagtt gcgtttgggt cacgttaaaa
     4861 taaatactcg cggagacccg ttcggaataa gcctgcgcaa tggccgcaaa tccggcaatg
     4921 agcacccggc tgcccggatc cgtatttgat ctgatccaga gcccgagcag tttctttgaa
     4981 taatcgtcct cctggttatt cattgaaggg ctgtctgggg atattgtttt ttccggcgac
     5041 ctgaaaagat ttttgaagct cccgatgggt tcccatattt tagggaaaaa gcagatcagc
     5101 accacgatca tggtttgcct ggccggtatg gaatagattt ccagcaaacg ctctatcgaa
     5161 agggccgtca ttaaggatag gggagctaac agatttttaa agtgctgctg ggaaaaaatg
     5221 cccagcaggc tgatgccaat gaattcacag atagaccaga taagaaaaag atcgtttctc
     5281 tttttaataa agaagtaccc ggccaggccg ggtaaaaaaa gcacctgttc ggaataaaag
     5341 aaatgattga agaaattgtt tattctccag gaaaaggatt gatctgtaac gctgcccgag
     5401 ccaaaattat c
//
LOCUS       DASYCU010000098         3959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2925287, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000098 DASYCU010000000
VERSION     DASYCU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2925287"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VGM24_01920"
     CDS             complement(<1..996)
                     /locus_tag="VGM24_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154144.1"
                     /translation="MLKNYFKTAWRNLSGNKVYSFINIGGLAVGMAVAMLIGLWMYNE
                     VSANKHFKNYDSICQVITHQTNGGEISTNWVTPFPLGDELKSKFNDFKAVAMCDGGAR
                     HSLQYDGKRVAKDGYFIGEEAVDMFSFNILYGDRNPLHDPYSIVLTDETARILFNTTN
                     AVGKIVKLDNAYDLKVTAVVARQPENSSFVFDCLLPWKLQERIYPDTKNYINDWGNNS
                     WRTFVQLNDQANVKNVNAKIRNVVLDHFTGDRIMLASKPQIELFPMSKWRLYTDFENG
                     KNVGGYIKYVRMFGILGLVVLLIACINFMNLSTARSSKRAREIGIRKTLGSLRKQI"
     gene            complement(1200..1724)
                     /locus_tag="VGM24_01925"
     CDS             complement(1200..1724)
                     /locus_tag="VGM24_01925"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY4154145.1"
                     /translation="MKKYFESAARFVLILCSVAFLSFALVDDSSFLSKASEANLAEID
                     AGKLAAAKAGSAWVKKYAEKMVADHTAAQAELAELAKKESATIPKEADSAHQILTKQL
                     AGLTGNAFDSAYIKSQLTDHQAAVSLFTEELTSGTDPDAVAYAKKYLPALKAHLAMIQ
                     QSGASMNILPDSTH"
     gene            1943..2266
                     /locus_tag="VGM24_01930"
     CDS             1943..2266
                     /locus_tag="VGM24_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4154146.1"
                     /translation="MNLRRDVFQAIADPTRRAILLLVTSQSMTAGAIASNFDTARPTV
                     SKHLQILTECELLEPTQYGREIYYYINAKKMKEVADFIEPFRKMWDDRFNKLESIMKK
                     YKPKK"
     gene            2273..2740
                     /locus_tag="VGM24_01935"
     CDS             2273..2740
                     /locus_tag="VGM24_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY4154147.1"
                     /translation="MEQKTKINAGDGKQDLTITREFDLPPELLFKALSEPGIIEQWMG
                     TKVLKLENKKHGGSQFETRDAEGNVVFKANGVFHEFVPNRKITRTFEMENAPFGVQLE
                     FLELEKLAEDRSKLNMHVVYESVAKRDQVLQLPFAAGINMAHDRLQDVVSKLK"
     gene            2747..3136
                     /locus_tag="VGM24_01940"
     CDS             2747..3136
                     /locus_tag="VGM24_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4154148.1"
                     /translation="MKKRDRIIYWISTLWLALGMVSTGAVQLIKGKTGPGGTDMMTQL
                     GFPLYFLTILGVWKFLGALAVLIPKFPVLKEWAYAGFFFAMSGAIVSHIASGTPNEIF
                     PAILLLILTLVSWYFRPLDRKTAPAIG"
     gene            3210..3785
                     /locus_tag="VGM24_01945"
     CDS             3210..3785
                     /locus_tag="VGM24_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HEY4154149.1"
                     /translation="MNKANSNKKGLPEKQRDEVLNGIKVRFEKNMNRHKSLKWAEIQA
                     RLEADPEKLWSLHEMETTGGEPDVVGQDKKTGEYIFYDCSAESPKGRRSFCYDHEALA
                     SRKENKPENSAVGMAAGMGIKLLTEEQYRDLQQMGNFDMKTSSWVSTPSDVRKLGGAL
                     FMDRRYDKVFLYHNGAESYYAARGFRGSLRV"
BASE COUNT         1075 a          919 c          972 g          993 t
ORIGIN      
        1 tatttgtttg cgcaatgaac cgagtgtttt gcggatgccg atctccctgg cccgttttga
       61 tgatcgcgcc gtgcttaaat tcatgaaatt gatacaggcg atcagcaaca cgacgagtcc
      121 caatattccg aacatccgca cgtatttgat atatccgccg acattttttc cgttttcaaa
      181 atccgtgtac agcctccatt tggacatggg gaacaattcg atttgaggct tggatgccag
      241 catgatccgg tcacccgtaa aatgatccag caccacattc ctgatttttg catttacgtt
      301 cttaacattt gcctggtcgt ttaactggac gaatgttctc cacgaattat ttccccaatc
      361 gttgatgtaa tttttcgtgt cgggataaat ccgctcctgt aatttccacg gaagcaggca
      421 atcaaaaaca aatgaactgt tttccggttg cctggcaacc actgcggtta ctttcagatc
      481 gtatgcatta tccagtttta caattttccc tactgcattg gttgtattaa aaagtattct
      541 ggcggtttca tcggtcagaa cgatcgaata cggatcgtgc aaagggtttc tgtcgccata
      601 cagtatatta aacgaaaaca tgtcaacggc ctcctcgccg ataaagtagc cgtccttcgc
      661 cactcttttg ccatcgtatt gcagggaatg cctggctcct ccgtcgcaca tggccaccgc
      721 tttgaagtcg ttgaattttg atttgagctc atcacccagg ggaaaagggg ttacccaatt
      781 ggtggatatc tcccctccat ttgtttgatg cgtgatcacc tggcaaatgg aatcgtaatt
      841 tttgaaatgc ttattcgcgg aaacttcatt gtacatccaa agcccgataa gcatggccac
      901 tgccatacct accgccagcc cgccgatatt gataaaggaa taaaccttat ttccggagag
      961 atttcgccag gctgtcttga aataattctt aagcattacc ttaagttaaa ttaaattcag
     1021 gaacaaaaaa cagccatcgg ctgaattgcc tgcgtggcgc ccttttcttc cggttattca
     1081 attaaataaa tgcgccgggg aaaagtcccg ggaaggccga cagagccttt aaattattgg
     1141 ttttcagcag cttttatatt tcacccggaa caaagacctt ttgcggaaag gcgcccggtt
     1201 taatgggtgg agtccggaag gatattcatt gatgcacccg attgctggat catagccagg
     1261 tgtgccttca gtgccggcag atactttttt gcataggcta cggcatccgg gtctgttccg
     1321 ctggtcagtt cctctgtgaa cagggacacg gccgcctggt gatccgttaa ctggcttttg
     1381 atatacgccg aatcgaaagc attgccggtt aggccggcca attgttttgt taagatctgg
     1441 tgtgcggagt ccgcctcttt cgggatggtc gcactttctt ttttagccag ttcggccagc
     1501 tccgcctggg ctgcggtatg atctgcaacc attttctccg cgtatttttt gacccaggca
     1561 ctccctgctt ttgccgcagc cagtttcccg gcatcaatct ccgccaggtt ggcctcgctt
     1621 gctttggaca ggaaggagga atcgtctacg agggcaaagg aaagaaaggc cacagaacag
     1681 agaatgagaa cgaaccgggc ggcggattcg aagtattttt tcataattcc tggttttctg
     1741 atcaatcttt caattcccat accaggggat ccggacacca atgaaaatgc ccgggagatc
     1801 cggattgacc tccggctttt ttggaaacag ggaaggtctt catccgggat ataccgcctg
     1861 tccatgggcg accgcaaaaa aatcccctga aatttgcgta gttaaataac tgcacatata
     1921 tttgcagtca aatgactacg caatgaatct gagacgggac gtatttcagg cgatagcaga
     1981 ccccaccaga agggccattc tgctgttggt aacctcgcaa tccatgacgg cgggtgccat
     2041 cgcctcaaac tttgatacgg ccagaccgac cgtttccaaa caccttcaga tactcaccga
     2101 atgcgaattg ctggaaccaa cgcaatacgg ccgggagatc tactattata tcaatgcaaa
     2161 gaaaatgaaa gaagtggcgg actttattga gccattccgc aaaatgtggg atgaccggtt
     2221 taacaaactg gaaagcataa tgaaaaaata caaaccaaaa aaatagaata ccatggagca
     2281 gaaaacaaag atcaatgccg gagacggcaa acaggattta acgatcaccc gggaatttga
     2341 tctgcccccg gaattgcttt ttaaagccct ttcagagccc gggatcattg agcagtggat
     2401 gggaacgaag gtgttgaagc tggaaaacaa aaaacacggc ggctctcaat ttgaaaccag
     2461 ggatgcggag ggaaacgtgg tttttaaagc caatggcgta ttccatgagt ttgtaccgaa
     2521 ccggaagatc acccggacat ttgaaatgga gaatgctcct ttcggtgttc agctggagtt
     2581 cctggaactg gaaaaactcg ctgaggaccg cagcaagctc aatatgcacg tggtgtatga
     2641 atcggttgca aaaagagacc aggtgctgca gttgcccttt gccgcgggaa taaatatggc
     2701 gcatgaccgg cttcaggacg tcgtcagtaa attaaaataa ccccatatga aaaagagaga
     2761 tcgaatcatt tactggattt ccaccctctg gcttgcattg ggaatggtct cgaccggggc
     2821 ggtacagctg atcaaaggaa aaacgggacc aggcggaacg gatatgatga cccaattggg
     2881 ctttcccctc tattttttga ccatactggg tgtttggaaa tttttaggcg ctttggccgt
     2941 gcttatacct aaatttcctg tactgaagga atgggcttat gcaggctttt tctttgcgat
     3001 gtccggagcg atagtttccc atatcgcatc gggcactccc aatgagatat ttcctgctat
     3061 cttgctgctg atcctgacgc tggtatcctg gtatttcaga cctttggaca gaaaaaccgc
     3121 tccggctatc ggataaagga acgaaagcat ttgaaataaa ttcaggtaat cgaaggccgg
     3181 cccatctggt gaaaagaaat aggtacagta tgaacaaagc aaatagcaat aaaaagggat
     3241 tgccagaaaa acaacgggat gaagtgctga acgggatcaa ggtccgtttt gagaaaaaca
     3301 tgaaccgcca taaaagcctc aaatgggcgg aaatacaggc aaggcttgaa gcggatccgg
     3361 aaaaactgtg gtcactccat gaaatggaaa caacgggcgg tgagccggat gtggtcggtc
     3421 aggataaaaa aacgggcgaa tacatttttt acgattgttc ggcagaaagt ccgaaaggcc
     3481 gcagaagctt ttgttacgat catgaagcgc tggcgtccag gaaagaaaat aagcccgaaa
     3541 acagtgctgt cgggatggcc gccggcatgg gcattaaact gttaacggag gaacaatacc
     3601 gcgacctgca gcaaatggga aatttcgata tgaaaacctc gagttgggtg agcacgcctt
     3661 ccgatgtccg gaagctcggc ggggccctct ttatggatcg ccgctacgac aaagtctttt
     3721 tatatcacaa cggcgccgaa tcctactatg cagccagggg attccgcgga tctttaagag
     3781 tctgagtgaa tgaaggcggc gatggagatc gcatacctga tttaaaacga ataaaaaaaa
     3841 catgggaatt cgcacccgct tgcccggcag cctgatctgc attggattcc ttcttatggg
     3901 cgcgtctttc cgggacagga ttccgcaaac cgctgcagcc ggagatcctc cgaaaatag
//
LOCUS       DASYCU010000099         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5100905, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000099 DASYCU010000000
VERSION     DASYCU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5100905"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="rpoB"
                     /locus_tag="VGM24_01950"
     CDS             <1..687
                     /gene="rpoB"
                     /locus_tag="VGM24_01950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611286.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY4154150.1"
                     /translation="KLKVGDKMAGRHGNKGIVAKIVRAEDMPFLEDGTPVDIVLNPLG
                     VPSRMNLGQIYETVLGWAGEKLGLKFATPIFDGATVEEIAEHIDKAGLPTFGHTYLYD
                     GETGDRFDQKATVGIIYIIKLHHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGE
                     MEVWALEAYGASNILQELLTLKSDDIIGRAKTYEAIVKGDSIPRAGIPESFNVLVHEL
                     RGLGLDLRFD"
     gene            complement(774..2075)
                     /gene="thrC"
                     /locus_tag="VGM24_01955"
     CDS             complement(774..2075)
                     /gene="thrC"
                     /locus_tag="VGM24_01955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY4154151.1"
                     /translation="MLFYNLRHPSEKVDFRTATIQGLGRDKGLYFPERIPVFPGEWIH
                     NIESFSNEEIAFSVIRPFVGDEIPEDVLRGIAAETIAFNIPLVPVSDRIAVLELFHGP
                     TLAFKDVGARFMSRCLGHFMRGSERPVTVLVATSGDTGGAVAHGFYDVEGVRVVILYP
                     SGKVSSVQELQLTSLGKNISALEVDGSFDDCQTLVKQAFSDKDLNESLFLTSANSINV
                     ARWLPQQIYYFLAWKQWKNKTRPPVISVPSGNFGNICAGLLARASGLPVAHFIAACNA
                     NAVVPEFLRSGDYKPVAAKVTMSNAMDVGDPSNFVRILELFNNQSGQLKKIMTGESVS
                     DEDTAATIRSVLATRGYLLDPHGAVGYLALERYLAAHPELSGIFLETAHPVKFFDVVE
                     KVTGQTVAVPAAISSLLASKKSAVSIRADYPRLKNFLLEAH"
     gene            complement(2099..>2628)
                     /locus_tag="VGM24_01960"
     CDS             complement(2099..>2628)
                     /locus_tag="VGM24_01960"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY4154152.1"
                     /translation="SGVKHADNIAPCIYGGITLIRSIFPLDIIPITPPPLFVTVVHPQ
                     IEVRTSDAREILRKEVLLKDAIRQWGNIAGLVAGLMKQDYELIGRSLEDVIIEPVRSI
                     LIPGFDEMKKLCRAAGAIGGGISGSGPSVFMLSANREIALAVEQAMRSVCDRIGLNYH
                     TYVTTISTQGVTCIV"
BASE COUNT          638 a          718 c          706 g          566 t
ORIGIN      
        1 aagctgaaag tgggtgataa gatggccggc cgtcacggta acaagggtat cgtagccaag
       61 atcgtgcggg cggaggatat gcccttcctc gaagacggaa caccggtgga tatcgtgctg
      121 aatccgctcg gcgtgccttc ccgtatgaac ctgggacaga tttatgagac cgttctggga
      181 tgggcaggtg aaaagctcgg attgaaattc gccaccccga tttttgacgg agccaccgtg
      241 gaggaaatcg ctgaacacat cgacaaggcc ggtttgccga cctttggcca tacttatttg
      301 tatgacggcg aaacgggtga ccgtttcgat cagaaagcca ccgtgggcat aatctatatc
      361 atcaaacttc accacatggt ggatgataaa atgcatgccc gcagcatcgg gccctacagt
      421 ctcatcacac agcaaccgct gggtggtaag gcccagtttg gcggccagcg tttcggagaa
      481 atggaagtat gggcgctgga agcttatggc gcatccaata ttcttcagga actgcttacg
      541 ctcaagtccg atgatatcat cggtcgcgcg aaaacgtatg aagccatcgt aaagggagac
      601 agcattccga gagccggaat acccgaatcc ttcaacgtac tggtccatga gttgcgcggt
      661 ctggggttgg atctgagatt cgattaaact ctgcttttac agaaaatata aagccggact
      721 gaatggtccg gcttttttta tgcctgctgt agcccacaga acctgaaaag aaattagtgc
      781 gcctccagca gaaaattttt cagccgcgga tagtcggccc tgatgctgac ggccgatttt
      841 ttgcttgcca gcagggatga tatggccgcg ggcacggcta ccgtttgccc cgtcactttc
      901 tccaccacat caaaaaactt taccgggtgg gccgtttcca gaaagatccc cgatagctcg
      961 ggatgggcgg ccagataccg ttccaatgcc agataaccaa ccgcgccgtg ggggtccagc
     1021 aaataacccc gggtggccag gacggaccgg atggttgccg cggtgtcctc atcggacacg
     1081 ctttcgcctg tcattatctt cttcagctgc ccggattgat tgttgaatag ttccagtatc
     1141 cggacgaaat tgctcggatc gccgacgtcc atggcgttgg acatcgtcac tttcgccgcg
     1201 acaggcttgt aatcaccgct gcggagaaat tcaggaacaa cggcattggc attgcaggcc
     1261 gcgataaaat gcgccacggg aaggccggaa gcccgggcca gcaagcctgc gcagatattt
     1321 ccgaagttcc cgctgggcac cgaaatcacc ggcggtctgg ttttattttt ccattgcttc
     1381 caggcgagaa aataatagat ctgctgggga agccagcggg ccacattgat ggagttggcc
     1441 gaagtgagaa acagggactc attcaagtcc ttgtcggaaa acgcctgttt tacgagcgtc
     1501 tggcaatcgt cgaaactgcc atcaacctcc agggcgctga tatttttgcc cagggaggta
     1561 agctgaagct cctgcacgct gctcaccttt ccggacggat aaaggatcac cacccgaacg
     1621 ccttccacat cataaaagcc atgcgcaacc gcgccgcccg tgtcgccgga ggtggccacc
     1681 agcaccgtta cgggccgttc gctgcccctc ataaaatgac cgagacatcg gctcataaac
     1741 cgcgcgccga cgtctttaaa tgccagggtg ggcccgtgaa ataattccag gacggctatc
     1801 ctgtccgaga ccggcacaag gggaatattg aatgcgatgg tttccgcggc gatcccgcgc
     1861 agcacatctt ccggtatttc atcacccaca aaaggccgga tcacggaaaa agcaatttcc
     1921 tcgttggaaa acgattcgat attgtgaatc cattctcctg gaaaaaccgg aatccgttca
     1981 ggaaaataca gccccttgtc ccttcccaga ccctgaatgg ttgccgttcg gaagtccact
     2041 ttttctgaag gatggcgaag attataaaac agcatgccgg ttgaaattaa tgatggttct
     2101 aaacaataca ggtcacgccc tgcgtgctga tggtggtaac gtaagtatga tagttcagtc
     2161 cgatccggtc gcacaccgac cgcatcgcct gctccacggc cagggcaatt tcccggttag
     2221 cgctgagcat aaacaccgac ggtccggatc cggaaatgcc gccgccgatg gctcccgcag
     2281 ccctgcacag ttttttcatc tcgtcaaaac ccggtatcag aatcgaccgc acgggctcga
     2341 tgatcacatc ttcgagcgat cgtccgatca gttcataatc ctgtttcatg aggccggcga
     2401 caagcccggc aatattgccc cactgccgga tggcgtcctt caaaagcact tctttccgca
     2461 ggatctcccg cgcgtccgaa gtgcgcactt caatctgcgg atgcaccaca gtaacaaaaa
     2521 gcggaggcgg agtaatggga ataatatcca gcgggaaaat ggaccggatg agggtaatgc
     2581 ccccatagat gcagggcgcg atattgtcag catgctttac gcccgaag
//
LOCUS       DASYCU010000100         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3505970, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000100 DASYCU010000000
VERSION     DASYCU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505970"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="VGM24_01965"
     CDS             <1..1398
                     /locus_tag="VGM24_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY4154153.1"
                     /translation="RKIELLKSNGFNAIRCSHNPPSEKFLETCDRLGMLVIDEAFDQW
                     EKPKNPDDYHRFFDEYWQKDFSSMILRDRDHPCVILWSLGNEIEERADPAGLEIAKKL
                     KSRANELDPSRAVTEAVCEFWDHPGRPWQDTAPTFALLDVGGYNYKWREYENDHRQFP
                     DRIMMGTESVPQEAYQNWQQVEKHPYVIGDFVWTAMDYLGESGIGHSRCDTGKDSQLE
                     PWPWFNAYCGDIDLAGNKKPQSYFRDIVWNRSKIEMAVGAPHSPGCKETVSFWGWPDE
                     QQSWTWPGNEGKTLNVTVYTRYPTIRLLLNGKIAGEKNLSDSSNLKASFQIPYQPGEL
                     KAEAIENGKEVAATALTTTGSAKSIRLVADRPEIRASRNDLAYVTVEVVDEKGNRVPN
                     ANIPLQFSISGNGELAGTGNANPADMASFKQPRRNSFRGRCLVILRPKGPAGTIILEA
                     RATGLQAAQITVHTK"
     gene            complement(1458..>2053)
                     /locus_tag="VGM24_01970"
     CDS             complement(1458..>2053)
                     /locus_tag="VGM24_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY4154154.1"
                     /translation="TDHENMLTWLFTFRNKATLAPVVVQDYVKLLAPFDPTHTGVDSL
                     RLGTEHHWNTVGLDFVSKPQSVFTYDFSLRYGGFYDRGTKFTFSNDVGYRFQPYVSIA
                     ISTSCNVLRLPQPWGNTTFWLIGPRIDVTMTNTLFFTAFVQYNQQVKNMNINTRLQWR
                     YKPASDLFIVYTDNYYSTPFSVRNRAVVLKFTYWWNI"
BASE COUNT          563 a          491 c          550 g          449 t
ORIGIN      
        1 cgaaagattg aattgctgaa atcaaatggc tttaatgcca tccgctgcag tcataatccg
       61 ccatcggaaa aattcctgga aacctgtgac cggctgggca tgctggtgat tgatgaagcc
      121 tttgatcaat gggagaagcc caaaaacccg gacgattatc accgtttttt tgatgaatac
      181 tggcaaaagg atttttcatc aatgatcttg cgggaccgcg atcatccctg cgttatcctg
      241 tggagcctgg ggaatgaaat tgaagagcgt gcggatccgg cgggcctgga gatagcaaag
      301 aaacttaaat caagggcgaa tgagcttgac ccttccaggg ccgttacgga agcggtttgt
      361 gagttctggg accacccggg acggccctgg caggatactg cgcccacttt tgccttgctg
      421 gatgtcggcg gctataatta taaatggcgg gaatacgaaa acgatcaccg gcaatttccg
      481 gatcgcatta tgatgggcac cgaatccgtt ccgcaggagg catatcaaaa ctggcagcag
      541 gtagaaaaac atccttatgt gatcggtgat tttgtgtgga cggcaatgga ttatctgggg
      601 gaatcaggca tcgggcacag ccgttgcgac accggaaaag attcgcaact ggaaccctgg
      661 ccctggttca atgcgtattg cggcgatatc gacctcgccg gcaataaaaa accgcaatct
      721 tatttccggg atattgtatg gaatagaagt aaaattgaaa tggcggtcgg cgcgccacat
      781 tcgccgggat gtaaggaaac ggtgagtttc tggggatggc cggatgaaca gcaaagctgg
      841 acctggcccg gcaatgaagg aaagaccctg aacgtgaccg tgtatacgcg ttatccaacc
      901 atccgtttgc tgctcaacgg aaaaatagcc ggagagaaaa acctttccga cagcagcaac
      961 ctgaaagcct cattccagat tccgtatcaa cccggtgaat taaaagccga agccatcgaa
     1021 aatggaaaag aagtggccgc taccgcactg accaccaccg gttccgccaa aagtatccgg
     1081 ctggttgctg accggccgga gatccgggca agccggaacg acctcgctta tgtaaccgtg
     1141 gaagtggtgg atgaaaaagg aaaccgggtt ccgaatgcaa acattccctt gcaattttca
     1201 atcagcggca acggagaact ggccggtacc ggcaatgcaa atcccgcaga tatggccagc
     1261 tttaagcaac cccggcggaa cagtttccgg gggagatgcc tggttatact ccggccgaaa
     1321 ggtccggcag gcactattat cctggaggca agggccaccg gactccaggc ggcgcaaatt
     1381 accgttcaca ctaaatgatt gtttaaaatc aggcggtatc gcgttctcac cggcggccgt
     1441 ttcattccgc caataattca gatattccac caataagtga acttcaatac cacggcgcgg
     1501 ttccggaccg aaaacggagt gctgtaataa ttatccgtat agacaataaa cagatccgat
     1561 gccggcttat agcgccattg aaggcgcgtg ttgatgttca tgtttttaac ctgctggtta
     1621 tactgaacaa aggcagtaaa aaacagggta ttggtcatgg taacgtcaat gcgcggcccg
     1681 atcagccaga acgtggtatt tccccagggt tggggtaacc ggagaacatt acaggaagtg
     1741 cttattgcaa tgctcacgta aggctgaaac cggtaaccga catcattact gaaagtaaat
     1801 tttgtacccc ggtcgtaaaa tcctccgtat cgcaaagaga aatcataggt gaatacactt
     1861 tgcggtttgg aaacaaaatc aagcccgacg gtattccaat ggtgctcggt tcccagccgc
     1921 agcgaatcga cgcctgtatg tgtcgggtca aatggggcca gcaatttgac gtagtcctgg
     1981 acgacaaccg gcgcgagggt ggccttattc ctgaaagtaa acagccaggt aagcatattt
     2041 tcatgatccg ttt
//
LOCUS       DASYCU010000101         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_315980, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000101 DASYCU010000000
VERSION     DASYCU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315980"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2354)
                     /locus_tag="VGM24_01975"
     CDS             complement(<1..2354)
                     /locus_tag="VGM24_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY4154155.1"
                     /translation="MNPPGIKSLFSFCRQCLWLGFFIFPSAGRGQKFNFLNYNVQQGL
                     VQSQVSGICQDASDNLWISTWGGISRFDGRSFSNFTETDGLANNFTNCIMASRSGFIW
                     VGTRDGLSRFDGSRFVNYRFTRWPSGNYVQSIAEDRSGVIWVRATNMIYTIDSWQRVL
                     PVNVTSIYDTVTALAADPQGTLWAAVFHKGIYRLEGQRWLLAIPLNPMHRNDHFLKII
                     FDRENRDRIFLLGKARLVSAEHGRIMESPSVLPYPKNDFYVDLFQDKSNKLWLLTRQA
                     LFQCVDFHPVLYSIGNGYTNNSTMAVFQDRENNIWFGTNGSGLMRYAGQPFIIYDQFN
                     TSKNAVIMYLLEGNDHRLYIGTDGSGVFVYDGDSMIPVRIPSEMALDKRIICLSKGSN
                     NEIYILTANSTLWKYADNRVTPVRLPILDYCTNAVLADEKGGFYVAGCHGCFYYSSSG
                     KTIRVLTAYCNNLLHIDRDSILVSALNGLYLVKDSQTFRKINFPGVDSCAFMSIKTLN
                     KFYLLATGNRGFILYNTITHATTRFTTKNGLNSDFVYSVVHDNGKQIWLGTGRGMNKL
                     VLDTANGTAEISDISTPGDISSSECNQNAAAYDYRQNLWFGTGSGLMEFFPVLGKKDL
                     YLPPITLQKLELFSQKIPAGKFSDSSSPEYQIPYHLSLPHDQNHLSFSYGCASFLNAD
                     KIVYQYRLEGMDKDFSAFTTNRAVTYSGLPPGRYTFRARAFLPGYGYSRNSIRYPFVI
                     SAAFYQTTLFRLLIILFGLCLVLWIQWLRMNHRIKRLKQIEAIK"
     gene            2496..>3056
                     /locus_tag="VGM24_01980"
     CDS             2496..>3056
                     /locus_tag="VGM24_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4154156.1"
                     /translation="MNDQPVIRIVIVEDDKTIREGYCFLIGKTPGYSIINSYASAELA
                     LKKLAADQPDLVLLDVGLPGMSGIEAIPKIRSLLPRVLVLILTVHESEEIVFQALSQG
                     ASGYLTKNSPSAKIMESIRDLANGGGSMSANIAQLVIKSFQRNAQSPLSKRETQILEE
                     IARGKNRRNIAEELFIDHETVKSHIKN"
BASE COUNT          863 a          722 c          727 g          744 t
ORIGIN      
        1 ttctttatcg cttcaatttg tttgagtctt tttatccgat gattcattct tagccattgt
       61 atccataaaa ccaggcacag cccgaataaa ataattaaaa gacgaaaaag agtggtctga
      121 taaaaagcag ccgaaatcac aaaaggatag cgaatggaat tccgggaata accatagccg
      181 ggtaaaaacg cccttgcccg gaatgtatac cgcccggggg gaaggcccga ataggtgacc
      241 gcccggttcg tagtaaacgc ggagaagtcc ttatccattc cctccagccg gtattggtaa
      301 acaattttat ctgcattcag aaagctggca cagccataac tgaatgacag atgattctgg
      361 tcatgaggaa gggaaagatg atatggtatc tgatattcgg gagaagaact gtctgagaat
      421 ttcccggcag gaattttttg tgaaaacaac tccagttttt gcagcgtgat cggcggaagg
      481 taaagatcct ttttccccag gaccggaaaa aactccatca gtcctgaccc ggtaccaaac
      541 cagagattct ggcggtagtc ataagcggcc gcattctgat tgcattccga agaggagatg
      601 tcgccgggcg tggagatatc tgatatctcc gccgttccgt ttgccgtgtc cagaaccagc
      661 ttgttcatcc cccttcccgt acccagccag atctgctttc cattgtcatg gaccacgcta
      721 taaacaaaat cggagttcag gccgtttttt gtagtaaacc gggtcgttgc atgggttatc
      781 gtattataga ggataaagcc cctgttcccg gttgccagca aataaaattt attcagcgtc
      841 ttaatgctca tgaatgcgca ggaatccact ccgggaaaat ttatttttct gaacgtctgg
      901 gaatctttca ccaggtacag accatttagc gcggaaacca ggatggaatc ccggtcgata
      961 tgcaacaggt tattgcaata agctgtcagc acgcggatgg tttttccgga ggaagaataa
     1021 taaaagcatc catggcatcc ggctacgtaa aaaccgccct tttcatcagc cagaaccgca
     1081 ttcgtgcaat aatccagtat gggaagccgg actggcgtta cccggttatc cgcatatttc
     1141 caaagcgtgg aattggcggt tagaatgtag atctcgttat tgctgccttt ggacaggcag
     1201 atgatccgct tgtccaaggc catttcagaa ggaatacgaa ccggaatcat gctatcgccg
     1261 tcgtatacaa atacacccga tccatccgta ccgatatata gccgatggtc attgccttcc
     1321 aacaggtaca tgatcactgc attcttcgag gtgttgaact gatcataaat tataaaaggc
     1381 tgaccggcat aacgcatgag cccggagccg ttggtgccaa accagatatt attttcgcgg
     1441 tcctgaaaaa cggccatggt gctgttattg gtgtacccgt tcccgatact gtaaagcacc
     1501 ggatgaaaat caacacactg aaaaagagcc tgccgggtga gcagccagag tttatttgat
     1561 ttatcctgaa aaagatctac atagaaatcg tttttcggat agggtaatac gcttggactt
     1621 tccatgatcc ggccatgttc tgccgaaacc agcctggctt tgcccaggag gaatatccgg
     1681 tccctgtttt cccggtcaaa aatgattttt agaaaatgat cattgcgatg cattggattc
     1741 agtggaatgg ccagcaacca tcgttgccct tccaggcggt atatgccttt atgaaaaaca
     1801 gccgcccaca aagttccctg aggatcggca gccaatgccg taacggtatc gtaaatcgac
     1861 gttacattga ccggtaaaac cctttgccag ctgtcaatag tatagatcat attggtagct
     1921 ctgacccaga tgactccgga acggtcttct gctatggatt gaacataatt tccggagggc
     1981 cagcgggtaa accggtaatt cacgaaccgg gaaccatcaa accgggagag cccgtcccgg
     2041 gtgcctaccc aaatgaatcc cgagcggctg gccataatgc aattggtgaa gttattggcc
     2101 agtccgtccg tttcggtgaa attgctgaat gagcggccgt caaaccttga tatgccaccc
     2161 caggttgaaa tccacaggtt atccgaagcg tcctggcaaa tgccactaac ctgcgactgg
     2221 acaagtccct gctggacgtt gtaattcagg aaattgaatt tttgtcccct ccccgccgaa
     2281 gggaaaataa aaaaacccag ccacaggcat tgcctgcaaa atgagaagag ggatttgata
     2341 ccgggtgggt tcatctggga aatattcata aaggccgaag ctcaccaaag taaaagataa
     2401 attaacttta tacgccttaa tctccataat aaatcccccg ttgaggggga ttttggaatg
     2461 aatgaaaagc cggaatttta taaaaaccga attgagtgaa tgatcagcct gttattcgga
     2521 tcgtaattgt ggaggatgat aagacgatcc gggaaggtta ttgtttttta attggcaaga
     2581 cgcccggata ttccatcatc aattcctacg cttccgccga actggcattg aaaaaactgg
     2641 cggctgacca gccggacctg gttttgcttg acgtagggct acccggcatg tccggcatcg
     2701 aagccatccc aaaaataaga tccctgttac ccagggtact ggtactcatt ctgaccgttc
     2761 acgaatcgga ggaaattgtg tttcaggcac tcagccaggg ggcctcggga tatctgacca
     2821 aaaacagccc gtctgcgaaa ataatggaat ctatccgcga cctggccaat ggcgggggtt
     2881 ccatgagcgc caatatcgcc cagctggtga taaaatcttt tcagcggaac gcccaatccc
     2941 ccctgtcgaa aagggaaacc cagatcctgg aagaaattgc ccgcggaaaa aacaggagaa
     3001 atattgcaga agaattattc atcgatcacg aaacggtgaa atcgcatatt aaaaat
//
LOCUS       DASYCU010000102         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2781461, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000102 DASYCU010000000
VERSION     DASYCU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781461"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGM24_01985"
     CDS             <1..243
                     /locus_tag="VGM24_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY4154157.1"
                     /translation="HPNLFHRSEANLSDHPRWSVISCYNLQSNLAYNETSVSWRMPVD
                     EVPDEALLEWEPESLSHADFLKKENDPALKETGWEK"
     gene            complement(257..329)
                     /locus_tag="VGM24_01990"
     tRNA            complement(257..329)
                     /locus_tag="VGM24_01990"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:Ala,seq:ggc)
     gene            390..578
                     /locus_tag="VGM24_01995"
     CDS             390..578
                     /locus_tag="VGM24_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6728 family protein"
                     /protein_id="HEY4154158.1"
                     /translation="MLIDFNMGMLRQIAEYLYLKKKDPNAPTSDFIRYMHGINRISIF
                     MFIIGILIILYRLFFRRH"
     gene            complement(580..1734)
                     /gene="lpxB"
                     /locus_tag="VGM24_02000"
     CDS             complement(580..1734)
                     /gene="lpxB"
                     /locus_tag="VGM24_02000"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY4154159.1"
                     /translation="MKYYLIAGEASGDLHGSNLVRELRKKDPAAEIRGWGGDLMQAAG
                     AEIVKHYRDLAFMGFTEVIRNLPVILRNIRFCKKDIESFQPDAVIFIDYPGFNLRIAG
                     WAKKSGYKTVYYISPQVWAWKESRVRTIRDCIDKMLVILPFEKAFYQKWNYAVEYVGH
                     PLVEVISNFKNSEDSRTPPDIIYPGLQKAAPDRPLIALLPGSRKQEILKKLPVMLEAS
                     RSFPDYQFVVAEAPGQEDSFYDEILKNYPDTGRVRNQTYSLLLHARAACVTSGTATLE
                     TALFAVPELICYKGSPVSYQIAKRLIRVKYISLVNLIMDKPVVKELIQDEMTSGNIVT
                     ELKKLLTDPETISRIRKDYADLKELLVQGGNASEKAAESIVRFCSASPSR"
     gene            complement(1782..2081)
                     /locus_tag="VGM24_02005"
     CDS             complement(1782..2081)
                     /locus_tag="VGM24_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154160.1"
                     /translation="MILKKDDLRLGIVMGIIAPLLAMLIYYFWNFSRSLTISEYFFLL
                     RTNKQLLTAISSISLLGNAVIFTVYINTHRDQTAKGIFVATLLYGIFVLVYKLVS"
     gene            complement(2078..>2485)
                     /locus_tag="VGM24_02010"
     CDS             complement(2078..>2485)
                     /locus_tag="VGM24_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY4154161.1"
                     /translation="IESIPSAGFSLLDHSVEADFTGARKYARIVVQQMLKTKLDKHTV
                     LNINIPAVPAELIKGIRICKQAYAKYEEDFVERNDPNGKKYFWLTGEFVNFDKGKDAD
                     VWALEHNFVSVVPVQFDLTNYAMKAKLEKSWKF"
BASE COUNT          625 a          618 c          590 g          652 t
ORIGIN      
        1 catcccaacc tgtttcaccg ttcggaagcc aatttatccg atcatccgcg ctggtccgta
       61 atttcctgtt ataacctgca atccaacctg gcatataatg aaacgtctgt ttcctggaga
      121 atgcccgtgg acgaagtacc tgatgaagcc ttactggaat gggaaccgga atccttatcc
      181 catgcggact ttctgaagaa agaaaatgat ccggcgctga aggagacggg ctgggaaaaa
      241 taaaagcctt aaagagtgga ggatatcggg ctcgaaccga ccacctcaaa catgccatgc
      301 ttgcgctcta ccagatgagc taatccccca aaaagagctg caaatatata caatccgaac
      361 aatccggaat aggtccttgt taaatatctt tgctgattga tttcaatatg ggaatgctaa
      421 ggcaaatagc tgaatatctt tatcttaaaa agaaagatcc gaatgcgccc accagcgact
      481 tcatccggta tatgcacggc ataaaccgga tctcgatctt catgttcatc atcgggattc
      541 tcattatcct ttaccgcctg tttttcagaa ggcactgatt taccttgatg gggaagcgga
      601 gcagaaccgg acaatgctct ccgccgcttt ttccgaagca ttaccgccct ggaccaataa
      661 ttctttcagg tcggcgtagt ctttccgtat ccggctgatg gtttccggat cggttaataa
      721 ttttttcagt tcggttacga tgttcccgga ggtcatttca tcctggatca gttcttttac
      781 caccggtttg tccatgatca ggttgaccag ggaaatatat tttacgcgga tcagcctttt
      841 ggcaatctgg taagaaacgg ggctgccttt gtaacagatg agttccggca cggcaaacag
      901 cgcggtttcc agcgtggctg ttccggaagt aacgcaggct gcccgcgcat ggagcaataa
      961 agaataggtc tggttcctga cccgtccggt gtccggataa ttctttaata tttcatcata
     1021 gaaggaatct tcctggcccg gcgcctcggc caccacaaac tggtagtccg gaaaggaacg
     1081 gctcgcttcc agcatcacgg gaagtttttt caggatctcc tgcttacggc tgcccggcaa
     1141 taaagcgatc agcggacggt cgggggccgc tttttgcagt ccgggataga tgatatccgg
     1201 aggggtccgg gaatcctccg aatttttaaa attcgagatc acttccacca gcggatgacc
     1261 cacgtattcc accgcatagt tccatttctg atagaacgct ttttcgaacg gaaggatcac
     1321 cagcatcttg tcgatgcagt cgcggatcgt ccttacccgg ctttctttcc aggcccagac
     1381 ctggggagaa atatagtaaa cggttttata tcccgatttt tttgcccatc ccgcgatgcg
     1441 gagattgaac ccgggataat cgatgaatat caccgcatcg ggttgaaagc tttcgatatc
     1501 tttcttacaa aaacggatat tccggagaat gacgggcagg ttgcggatca cttccgtgaa
     1561 tcccataaag gcgagatcgc ggtaatgctt cacgatctcc gctccggcgg cctgcatgag
     1621 atcgcctccc cagccccgga tttcggcggc gggatccttc ttccttaatt cgcggaccag
     1681 gttgctccca tgcagatccc cagatgcttc cccggcaatg aggtaatatt tcatccgtta
     1741 atttgtcttg agataaaatt tatcatgtac gattcctgaa actagctgac cagtttatat
     1801 accagcacaa aaataccata gagaagggtg gcaacaaaaa tgcccttggc cgtttgatcg
     1861 cggtgggtat taatataaac agtgaaaatg acggcgttgc ccagaagaga aatgctgctg
     1921 atggctgtca gcagttgttt attggttctc aaaagaaaaa aatattcgga aatcgtgagc
     1981 gagcgggaga aattccagaa atagtagatc agcatagcca gcaaaggggc aatgatgccc
     2041 attacgatgc ccagcctcag atcgtctttt tttaatatca aaatttccag gatttttcga
     2101 gtttggcttt catcgcataa ttggtcaggt cgaattgcac cggcaccacg ctgacgaaat
     2161 tatgctccag ggcccacacg tccgcatcct tgcctttatc aaaattcacg aattcaccgg
     2221 taagccagaa gtattttttc ccgttgggat catttctttc cacaaaatcc tcttcatact
     2281 tggcatacgc ctgtttgcag atccggatcc ccttgatgag ttcggccggc accgccggga
     2341 tattaatatt gagtacggtg tgcttgtcca gtttggtctt gagcatctgc tgcaccacga
     2401 tgcgggcata tttcctggcc ccggtaaaat ccgcttccac gctgtggtcc agcagggaaa
     2461 agccggcgga gggaatgctt tctat
//
LOCUS       DASYCU010000103         4327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1185052, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000103 DASYCU010000000
VERSION     DASYCU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185052"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..866
                     /locus_tag="VGM24_02015"
     CDS             489..866
                     /locus_tag="VGM24_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY4154162.1"
                     /translation="MKKRTGINPWMFRLEMAVLYYAFRDLRTPFYAKIPAVISLIYLL
                     SPVDLIPDFIPLVGYLDDLIIVPFLISASIRLLPKEVKIESLAKARKKTRQINLVFAG
                     LILLIILLLGGIFFAFQHLWRTG"
     gene            complement(1048..1494)
                     /locus_tag="VGM24_02020"
     CDS             complement(1048..1494)
                     /locus_tag="VGM24_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154163.1"
                     /translation="MPARPGMKVMYQNASSAEKKKPFQDWLREARSLEDDETAEQAIA
                     AYEKIRRAYPLKEESYDRLMVLYRKTKRYKEEIKLIGEAISIFETRYGTLKKKVKGKI
                     ATLSKALLNTTGLADKKGRPLYQMQPVGRWQKRKLLLQEKLKKATG"
     gene            complement(1556..1738)
                     /locus_tag="VGM24_02025"
     CDS             complement(1556..1738)
                     /locus_tag="VGM24_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154164.1"
                     /translation="MPLNPEILAQRIAELTKAYQRQVDKAGHPEYDPALTELLKKDLT
                     WLQDQQEQGEQVPKEK"
     gene            2259..2693
                     /locus_tag="VGM24_02030"
     CDS             2259..2693
                     /locus_tag="VGM24_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154165.1"
                     /translation="MNKRKPRFDIVNEILEECILAYPISPFIISLYKQYQQRGSLSKK
                     QLQGLYGKASKIKHLPPGKLATIEAIIKKMTTRHKAEIPIGEPLFKKEEAVGEMIAEI
                     LERYPQHKRVLFLKSKYDNNEPLSSSDLADLKKFRQILIEPR"
     gene            complement(2720..3181)
                     /locus_tag="VGM24_02035"
     CDS             complement(2720..3181)
                     /locus_tag="VGM24_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154166.1"
                     /translation="MRSKFLLTAVFFSAVLYSCKKDKTAPVSAQTLMLLQNKWALVSE
                     VTVVPTQPTYNTTYTGAPTDYYLFSTEDTLFVHKRSPVYSYMLPITDTLPYSVRNDHT
                     LAYGPNTNVEIIIEKLTTDSLILNNPVTVSFVNVDNSVTSYSGTKTITLAR"
     gene            complement(3253..>4327)
                     /locus_tag="VGM24_02040"
     CDS             complement(3253..>4327)
                     /locus_tag="VGM24_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY4154167.1"
                     /translation="GLAHFKPTDLSKLLSGRQLSVSPYISERLEGIRGTSTPKDLETA
                     LQLVHLYFTSPRKDSTIFRNIIDQSITAIANRNTDPNSVFADTVSAVLGDHSIRRTGP
                     TVEKLNQIDLEKSLAIYKERFANAGDFTFVFDGNFSVDSIRPLLEKYLGSLPGQPQKE
                     SARDLKIHIPTGKIEATAIKGREPKASVRLVVSGDYQYSPENNIQLTALGEILQFRII
                     DRLREKEGGTYSPGVRVTYNKYPANRYAFTISFGCAPDNVEKLINDTKEEIAKLKTSG
                     VTALDITKFSTEEQRQYELHLKDNGFWLNWLIAQYENGDDPATILKYPELIKQVNPAS
                     VKAAANLYLNEDNFIKLVLLPEK"
BASE COUNT         1110 a          977 c          971 g         1269 t
ORIGIN      
        1 atcaggcggc cgccggaacg attaccggtt gatggtcctg accgtaacca catcctgccg
       61 gatcagaaaa aatcgcaaaa accgtttggt tctgcctgat ctttccgcga agaattcgcc
      121 ctggctccgg agtgaaccgg actttttaga aagctttcgg tatgctctct taatttcgtt
      181 cggactggca ttgaatgata ttccaagctg ttgtagataa tctggtaaag tcatttcagt
      241 gtgcatgggt ttaaataggg tttttaaaaa acggccttag ccggaagtta ttatttatga
      301 cctggaatat ttcctttatt tggagcggag ctccaaacgg caaggcgaaa tgaaaggcag
      361 gtgtagtggt ttccactaca gggttaccct gctttattaa gcccgcccgg gccacaactc
      421 attattgaac tattgttttg tattttagaa ggagaatatt cttgtagaaa ataaccaaaa
      481 gccctggctt gaaaaaacgg accggaataa atccatggat gttcaggctg gaaatggccg
      541 tgttgtatta tgcattccgg gatttgcgaa ccccttttta tgcaaaaata ccggctgtca
      601 tttccctgat ctacctgctt agtccggtag atctcattcc agattttatt ccccttgtcg
      661 ggtacctcga tgatctgatc attgttccgt tcctgatcag tgcctcgatc cgtttattac
      721 ctaaagaagt aaaaatagaa agtcttgcaa aagcaagaaa aaagaccagg caaataaacc
      781 tggtttttgc cgggctgatc cttctgatca tcctattgtt gggaggtata ttttttgcct
      841 ttcaacattt atggcggacg ggataatcct gccattgaaa gggagaatca ataaacagtg
      901 tgtttttttt gctaacttta tccttcatca aagaaaagga ggtattcatg aaatctacag
      961 tcaattcatg gaaacgtccc gttagtttcg tctaaccagg atttttcata aaatagctgt
     1021 ggcgccacgg gcgccgaagc cataggttca tcctgtggct ttttttagct tctcctggag
     1081 caataatttt cgtttttgcc agcgtccgac cggctgcatt tggtaaagcg gacggccctt
     1141 cttatccgcc aggccggtgg tattaagcaa tgccttactg agggtagcta ttttcccttt
     1201 cacttttttt ttcagcgtcc cataccgggt ttcaaaaatg ctgatggctt ccccgatcag
     1261 cttaatctct tctttgtacc ttttcgtttt ccggtagaga accatcagcc ggtcataact
     1321 ttcttctttc agcggatagg cccttcggat cttttcatac gctgcaatag cctgttctgc
     1381 tgtttcatca tcttccagcg atcgcgcttc ccggagccag tcctggaatg gttttttctt
     1441 ctctgcggaa gaggcattct ggtacatcac tttcataccc ggtctggctg gcattttaaa
     1501 cgtaactgtt taaaaaatta tgccattctg agcggcccac cggcaggagt ccgtattatt
     1561 tttctttggg aacctgttct ccctgctcct gctggtcctg cagccaggta agatcctttt
     1621 tgagcaattc ggtgagcgcc gggtcgtatt cgggatggcc ggccttgtcc acctggcgct
     1681 ggtaagcttt cgtaagttcg gcaatccgct gggcgagtat ttcggggttt aaaggcatat
     1741 cgcaatatcg tgaaaatttt gtttcagcaa aggccattta taaaaaccgg ccggtttacc
     1801 agtttattgg ctgttcccgg ttggggcaag ccaaaataaa acagcccttt tcagggccgt
     1861 ttgaatattt caggcgttta ataagaaaga agaaaagccg gcactttccc cggttattcg
     1921 ttgaatttaa acggataacg ggatctgctc tttactgtgt ttcaaggata cgcctatttg
     1981 ctggtttttc aatggatccg gattaccggt ttctcaggaa tggataggat tcggtttttt
     2041 ctgacggata ctgattttca ggtttttcag gatttaattt ttcaggggat ttccggatta
     2101 tgtatttagt aaccggaggg aaagcccgcg taaccgcttt tgccttttat tttatatttt
     2161 ataaataact gattttcaat aatattcctt actctccccg cctcccgggc agacaaaagc
     2221 ataaaacatt atttattttt gccttctttc gatcataaat gaataaacgg aaacccaggt
     2281 ttgatatagt caatgagata ttggaagaat gcattttggc ctaccccatt tccccgttta
     2341 ttatcagtct gtacaagcaa taccagcaga gaggaagcct cagcaaaaaa caactgcagg
     2401 gtctctatgg aaaagcgtca aaaataaaac atctccctcc gggcaaactg gcaaccatag
     2461 aagctataat aaaaaaaatg accacccggc ataaagccga aatacccatc ggagaaccct
     2521 tattcaaaaa agaagaagct gtcggcgaaa tgatcgcgga aatactggag cggtacccgc
     2581 agcataaaag ggttctgttt cttaaatcaa aatacgacaa caacgaaccg ttatcttctt
     2641 ccgacctggc ggatctgaag aaattcaggc aaatattaat tgagccgcgt tagttgccaa
     2701 cgtccgccgg gaaacgacgt tatctggcta aagtaatggt ctttgttccg gagtaactgg
     2761 taacggaatt gtccacattc acaaatgaaa ccgttacggg attatttaga atcagcgaat
     2821 ccgtggtaag tttctcaata ataatttcga cattggtatt cgggccatag gccagggtat
     2881 ggtcattccg gaccgaatac ggcagggtat cggttatcgg cagcatgtag gaatagactg
     2941 ggcttcgctt gtgaacaaaa agggtgtcct ccgtgctgaa caaataataa tcggtcggcg
     3001 cgcccgtata cgtggtatta taggttggct gcgtgggcac cacagtcact tccgaaacca
     3061 gtgcccattt attttgcaat aacattaagg tctgagcgga aaccggcgcg gttttatcct
     3121 tcttgcagga ataaagtacc gcggaaaaga aaacagccgt cagtaaaaat tttgatctca
     3181 taatcaactg aattaaagca aaaatttaaa gccgggcaac agccagcggc tcataaaaag
     3241 cggatccggt aattattttt ccggcagcaa taccagttta ataaaattat cttcgttcag
     3301 gtaaaggttt gcagcggctt taaccgatgc cggattcacc tgtttgatta attccggata
     3361 ttttaatatc gtagccggat cgtccccgtt ttcatattgg gcaatgagcc agtttagcca
     3421 aaacccgtta tcctttaaat gcagttcata ttgccgctgc tcttcggtgg agaatttggt
     3481 aatgtcgagg gccgttacac cgctggtctt taatttagcg atttcttctt tggtgtcgtt
     3541 gatcagtttc tccacattat ccggcgcaca accaaatgaa atggtaaagg cataccggtt
     3601 tgccgggtat ttattatagg ttacccgcac tcccggacta tacgtgcctc cttctttttc
     3661 tcttaagcgg tcgatgatcc ggaactgcag gatttctccg agagccgtca actgaatatt
     3721 gttctccgga ctgtactgat aatctccgct gaccaccaaa cgcacggaag ccttcggttc
     3781 cctgccctta atagccgtgg cttcgatttt tcctgtcgga atatgaattt tcaggtcgcg
     3841 tgcggattcc ttttgcggct ggcccgggag ggaacccaaa tatttttcca gcagcggccg
     3901 tatgctgtcc accgaaaaat tcccatcgaa cacaaaggta aaatcacccg cattggcaaa
     3961 gcgttctttg tagatcgcaa gcgatttttc caggtcaatc tgatttaatt tttcaacggt
     4021 ggggcctgtt cgccggatgg agtgatctcc cagcaccgcg gacaccgtat ctgcaaatac
     4081 actattcgga tcagtattcc ggtttgcgat cgccgtgatc gattgatcaa tgatattccg
     4141 gaaaatggta ctgtcctttc tgggagaagt aaaataaaga tgaaccaact gcagggcagt
     4201 ctccaggtct ttcggtgtgc tggttccccg gataccttcc aatctttcgg aaatataagg
     4261 cgaaacgctc aattgcctgc cgcttaacag cttcgagaga tccgtcggtt tgaaatgcgc
     4321 cagtcct
//
LOCUS       DASYCU010000104         7230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4231505, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000104 DASYCU010000000
VERSION     DASYCU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4231505"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VGM24_02045"
     CDS             <1..310
                     /locus_tag="VGM24_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEY4154168.1"
                     /translation="LVSYVIYHSTNPTVRFGDINHDGMLSPDEKQRAGNLRYVYYIVL
                     SSHIILSAVIIPFVLFTLQKAFQEKFLRHKKIARITWPMWFYVAVSGVVVYWMISPYY
                     "
     gene            complement(318..707)
                     /locus_tag="VGM24_02050"
     CDS             complement(318..707)
                     /locus_tag="VGM24_02050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY4154169.1"
                     /translation="MASKKPVPKLKLKDLKPSGKQDQSFFEQVYEVARQIPAGRVTSY
                     GAIAACLGAKSSARMVGWAMNGADNVSPTVPAHRVVNRVGLLSGKIHFKPPEKMERLL
                     KKEGVLVKKDTVVHFREKFWDPGKELL"
     gene            complement(739..1452)
                     /gene="trmB"
                     /locus_tag="VGM24_02055"
     CDS             complement(739..1452)
                     /gene="trmB"
                     /locus_tag="VGM24_02055"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY4154170.1"
                     /translation="MAQKKLQRFQAIRSFKNVLEYPENMAGKWGDFFKNENSITLELA
                     CGKGEYTVALSALHPEENFIGVDRKGNRIYTGASRCLQNNQHNAAFLRTQIENIDGYF
                     LPGEVSAIWITFPDPQLRTSRAKKRLTHPRFLRLYQKILKPGGQIHLKTDSPVLFHFS
                     RLIVDLYQLPVLASVEDLYAASDIPEELRIKTHYEALDIAGSRKIYYLQFSLPEEPLP
                     AGDARLQELLKEMEKPVLS"
     gene            1531..2073
                     /locus_tag="VGM24_02060"
     CDS             1531..2073
                     /locus_tag="VGM24_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154171.1"
                     /translation="MTYFSELYRHNKWLCLGVAVFCALTIAANLLKVEATPVFIWGMY
                     SDKETAPQEYRILKVVVNDSIPINYSAGYPDATRFYLLSPLTYYWSIVENDGKDPEET
                     YYREKLGKYFKLLEPVRKHLFNDSGSTSPFIKWYAGYLEEVTRIPVSRLEVTVLTVAY
                     HGAGRPEIQQKSTLVKWSRP"
     gene            2058..2897
                     /locus_tag="VGM24_02065"
     CDS             2058..2897
                     /locus_tag="VGM24_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154172.1"
                     /translation="MEQTLEEQPSVFPGLRDRLCRITFSLILISLIYSFYSHTLTSQL
                     LKPVLIFPSNDLTYWVIHLTRLPAIITGHLAIAVAFDLILFLSCVWCILHPQRRVWPV
                     LFFIFYLLYFVIFNSYGLFHAHKRVGVLLLPIPFMMAGDRSFLLIWKGLRYYVCYIYG
                     TAFLWKLFRGSGFYGQQAVLIIKRNLTAYLYYDPGSLLSKIYSWMILHPQVPALLSAA
                     GFMLEGLFIIGFFTRRMDRGLFLLSIILPLGFLFMADTLFLELIFLAICFYDLPAGKQ
                     VRR"
     gene            3267..3740
                     /locus_tag="VGM24_02070"
     CDS             3267..3740
                     /locus_tag="VGM24_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY4154173.1"
                     /translation="MASKFNLDKLDLQIIHEMMENAEISYADLGKKLFVSGGTIHVRI
                     KKLQEFGIVKGTKLNVNLKHLGYDVIAFIGIYLEKSSLYDSVAKELRRIPDIVRLNYT
                     TGNYSMFAEIVCKDITQLRYVLHDELQKIKGIERTETFISLDESFTRNVQVYKEP"
     gene            complement(3768..4850)
                     /locus_tag="VGM24_02075"
     CDS             complement(3768..4850)
                     /locus_tag="VGM24_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154174.1"
                     /translation="MSNANPIQSSLWRRLRKNPGAVFGIVLISISVLVAVFAYVLAPD
                     HSPDANRMTLEIGGQKPGFHQSFLLIRKPRPPVSKSRIAAWFTGEDDRYEYIPIDSWK
                     QERDSLIVNKFIDEGIVERRSYFHPPADPGMQIKKKTFWLGTDRYGRDILSRLIIGVR
                     VSLAVGLITVLLSLSIGILLGALAGYFGGRTDHILMWFLNVIWSIPTLLLVFALTLLL
                     GKGFWQVFIAIGLTMWVSVARIVRGQVLAVKQLEYVEAARAMGYSTFRIIGKHILPNI
                     MGPVLVIAASNFASAIVIEAGLSFLGIGVQPPQPSWGLMIKENYNFIITHNPMLALVP
                     GFAIMLLVLAFNLLGNGLRDALNVKE"
     gene            complement(4956..6086)
                     /locus_tag="VGM24_02080"
     CDS             complement(4956..6086)
                     /locus_tag="VGM24_02080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4154175.1"
                     /translation="MPIFIAITGALLLLYSLLILYYHWIWDRIPPSKNGDPVPGVTVS
                     VIIPARNEELHIGLCLQTILRQTYPSSLTEIIVVNDFSTDRTASIVEQYSGRVKLLDL
                     KDFVSGDDLNAYKKKAIETGIAQSSGELIACTDADCIARPEWLETLVSAYTGWHCQIL
                     AAPVKIETTGSALSVFQALDFISLQGITGAAVYKDLYPMCNGANLAYPRRAFTEVGGF
                     SGIDHIASGDDMLLMGKIQSRFPGRAAFLKDPKAIVSTRPAADLRSFLRQRIRWASKV
                     SHYGHPFIFLTLALVYALNACLLLLFLLSFYYPRWHWLVFYLVLKCFAEFFFVSRVAG
                     FFEQRPLMKYFIFCQPFHILYTVIAGAFGAFGKYHWKDRRVK"
     gene            6239..6679
                     /locus_tag="VGM24_02085"
     CDS             6239..6679
                     /locus_tag="VGM24_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY4154176.1"
                     /translation="MPNNHFKKGELYSFVTGKASIAIARRLQKKFNQAGLNITIEQWS
                     VLYQLWKQDGQSQQELCRATFRDKPSITRLLDNLEKLQLVKRVASSNDRRINRICLTK
                     LSFKLQEQTMLLAEDTLNEALERVPGEQVEVCKSVLQIVYDNLK"
     gene            6701..>7230
                     /locus_tag="VGM24_02090"
     CDS             6701..>7230
                     /locus_tag="VGM24_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4154177.1"
                     /translation="MSTATTKPEQIKGGEWLIRESAGPDIFIPEDFSEEQRMVKEMCA
                     SFLDTEVLPFMDRIDKLEPGLMPSLLVKAGEQGLLGASIPENLGGLGKDFVTSTIINE
                     GLGGGYSFSVALAAHTGIGTLPILYFGTEAQKEKYIPKLASGEWKASYGLTEPNSGSD
                     ALSAKTTATLTKDGKY"
BASE COUNT         1847 a         1796 c         1799 g         1788 t
ORIGIN      
        1 cctggtatcc tatgtgatct atcacagtac aaaccctacg gtaagattcg gggatatcaa
       61 tcacgatggg atgttaagcc cggatgaaaa acaacgcgcg ggcaacctgc gctatgttta
      121 ttatatcgtg ttgagctcac atattatctt atccgccgtg atcattcctt ttgtattatt
      181 caccctgcaa aaggcttttc aggaaaaatt cctgcggcat aaaaaaatcg cccgcatcac
      241 ctggccgatg tggttttatg tagctgtgtc gggggtggtg gtttactgga tgatcagccc
      301 gtattattga tttatcctca aagcaattcc ttgccggggt cccagaattt ttccctgaaa
      361 tggacaacgg tatccttttt aacgagcacc ccttcttttt ttaataagcg ttccattttt
      421 tcggggggtt tgaaatgaat tttcccggag aggaggccaa cccggttcac cacccggtgc
      481 gcaggtacgg tcgggctcac attgtccgct ccgttcattg cccagcccac catacgtgcg
      541 gaacttttag caccgaggca ggcggcaatg gcgccgtaac tggttacccg gcccgcgggg
      601 atctgccgcg ctacttcgta aacctgttca aagaacgact ggtcctgttt cccggaaggc
      661 ttcaggtctt tgagcttgag tttcggaacc ggttttttgg aagccataca gatgatttta
      721 aataagcttg ttcttcaatt aggaaagtac cggcttttcc atttctttca aaagttcctg
      781 caaccgggcg tcgcccgctg gcaggggctc ctccggcaat gaaaactgca ggtaataaat
      841 cttcctggac cccgctatat ccagggcttc ataatgcgtt ttaatccgga gctcttccgg
      901 aatatcagac gccgcataca gatcttcaac gctggcaaga accggaagct ggtacagatc
      961 aacgatcagc ctgctgaagt gaaacaggac gggagaatct gttttcagat gaatttgtcc
     1021 gcctggtttt aaaattttct gatagagccg gagaaagcgg ggatgggtta accttttttt
     1081 tgcgcgcgaa gtgcgtagct ggggatcggg aaaagtgatc cagatcgcgc tgacctcacc
     1141 cggtaaaaaa tagccatcga tattttctat ctgggtgcgc aggaaagcgg cattatgctg
     1201 gttattttgc aggcaccggg acgctccggt atagatgcgg ttccctttcc ggtccacgcc
     1261 gataaaattc tcttccggat gcagggcgga aagcgccacg gtgtattctc cttttccgca
     1321 ggccagttcc agggtaatgg agttttcgtt cttaaagaaa tcaccccatt tccccgccat
     1381 gttctccggg tactccagga catttttaaa cgacctgatg gcctggaagc gttgaagttt
     1441 tttctgtgcc atgacctgca aaaataaggc ttgacgggga acctgattat ttaaattacc
     1501 ttagccagac tcatttaccg ggaagcaggg atgacctatt tttcagaact atatcgtcat
     1561 aataaatggc tttgcctggg cgttgccgtt ttttgcgcac tgaccatcgc ggcaaacctg
     1621 ttaaaagtgg aagccactcc tgtttttatc tggggaatgt attcggataa ggaaacggca
     1681 ccgcaggaat accggatatt gaaggtggtt gtgaatgaca gtattcccat aaactatagt
     1741 gccggttatc cggacgccac gcggttctat cttctctctc cgctcacgta ttactggtct
     1801 attgtggaga atgatggaaa ggatcccgaa gaaacctatt accgcgaaaa gctgggaaaa
     1861 tattttaagc tgctggaacc cgtaagaaag catttgttta atgattccgg gagtacgtcc
     1921 ccatttatta aatggtatgc cggctacctg gaagaagtta cgcgtattcc ggtcagcaga
     1981 ctggaagtga cggttttgac ggtggcatac catggagcgg gaagaccgga gattcaacag
     2041 aaatccacat tggtaaaatg gagcagaccc tagaagagca gccatcggtg tttcccggcc
     2101 tcagagatcg cctctgcaga attacctttt ccctgattct catctcgctg atttattctt
     2161 tttattccca taccctcacg tcccagctcc tgaagccggt ccttatattt ccgtcaaacg
     2221 atctgaccta ctgggttatt catctgaccc gcctgcccgc tatcattacg ggccatttgg
     2281 cgatagctgt tgcgtttgac ctcattttat tcctgagttg tgtatggtgt atcctccatc
     2341 cgcaacggcg tgtatggccc gttctttttt ttatatttta tctcctctat ttcgtcattt
     2401 tcaacagcta cggattattt cacgcgcata aacgggttgg cgtattgtta ttgcccattc
     2461 cttttatgat ggccggcgac cgcagctttt tactgatctg gaaggggttg agatattatg
     2521 tgtgctatat ttacggcact gcgtttttat ggaaattatt ccgaggctcc gggttctacg
     2581 gtcagcaggc cgtactgatc attaaaagga atctgacggc ctatttatat tatgatcccg
     2641 gatcccttct ttcaaaaata tattcctgga tgatcctgca tccgcaggtg ccggccctgc
     2701 tatcggcagc gggatttatg ctggaaggcc tgtttattat cggatttttt acccgccgga
     2761 tggaccgggg actctttctt ctttcgatta ttttaccgct gggatttctg ttcatggccg
     2821 atacgctgtt tctggagctg atctttctgg ccatctgttt ctacgatctt ccggccggca
     2881 aacaggtccg gcggtaaagc aaaaaccctg aaacccggat acgatccgga ttccagggct
     2941 tttcaagctg tagagggagg attcgaacct ccacggggca gttagccgta gcacaatgtt
     3001 agtggtggtc aaccccagtc ggccacgctt tgcagcgagg acggcattat gctatgttta
     3061 tcctgtgatc cccacccccg agacaagagg gcatgtctgc caaagttcca tcactccaca
     3121 gtaaagtcac tgcgctttcg caggaacatg caatattaag gtaaatcggt gaattattga
     3181 attatattta ataaaaattt tgaaatattc gaatatattt aaatattatt tatatttatt
     3241 gaatattctt aaaaaccctc tttaaaatgg caagtaaatt taacctggac aagcttgacc
     3301 tgcagatcat tcatgaaatg atggaaaatg cagaaatctc ttatgctgat cttggaaaga
     3361 agctttttgt ttcagggggt acgatccatg tgcggataaa gaagctgcag gaattcggta
     3421 tcgttaaagg tactaaactg aatgtaaatc taaaacattt ggggtacgac gtgattgcct
     3481 ttatcgggat ctatcttgaa aaaagttctc tgtatgattc ggtggcgaaa gagttgcgga
     3541 ggattcccga tatcgtgcgg ttgaactaca ccacgggcaa ctacagcatg tttgcggaga
     3601 tcgtctgcaa ggatatcacc cagctccggt atgtgctgca cgacgaactg caaaaaataa
     3661 aaggcattga acggaccgaa acattcattt cgctggatga aagtttcacc cggaatgtcc
     3721 aggtttataa agagccctga tcgcaggcgg agatccttcc catttcttca ctctttcaca
     3781 ttcaaggcat cgcgcaaccc gtttcccagc aaattgaagg cgagcaccag cagcatgatg
     3841 gcaaagccgg gaaccagcgc cagcatagga ttatgggtta taatgaaatt ataattctcc
     3901 ttgatcatca gcccccagct gggttgcggc ggctgaacgc cgatacctag aaagctgagc
     3961 ccggcctcaa tcacgatggc cgaggcgaaa ttggacgctg cgatcaccag cacgggtccc
     4021 atgatattgg ggaggatgtg ttttccgatg atccggaacg ttgaatatcc catcgcacgc
     4081 gccgcttcca catattccag ttgcttcacg gcgagtacct gtccgcgcac gatacgcgcc
     4141 acgctcaccc acatcgtcag tccgatcgca atgaacacct gccagaagcc cttgcccagt
     4201 aaaagggtga gggcaaatac gaggagcaag gtgggaatgc tccagatcac attcagaaac
     4261 cacatcagga tatgatcggt ccttcctccg aaataaccgg ccagggcgcc cagcaggatg
     4321 ccgatgctga gcgagagcag tacggtgatg aggccgacgg ccaggctgac ccgcacgccg
     4381 atgatcagcc ggctgaggat atccctgccg taccggtccg tgcccagcca gaatgttttc
     4441 tttttgatct gcatgccggg atccgccggt ggatgaaaat acgaccggcg ttcgactatg
     4501 ccttcatcaa taaatttatt cacgatcagg ctgtcccgct cctgcttcca ggaatcaatg
     4561 ggaatatatt cataacggtc atcctcaccc gtaaaccagg ctgcgatccg ggatttggag
     4621 accgggggcc gcggtttccg gatcagcagg aaggattgat gaaaacccgg tttttgtccg
     4681 ccgatttcca gcgtcatccg gttggcatcc ggactgtggt cgggggccag tacatacgca
     4741 aacaccgcaa ccagtaccga aatggaaata agtacgatgc cgaaaaccgc gcccggattt
     4801 ttccttagcc gccgccagag ggaagattga attgggttag cgttggacaa tgcgttgccg
     4861 gtttacattc atattgatcc gcgttggagc ttgcaagttc gaaataaatc cggatattca
     4921 tccaccgccc gctggctgaa tgatccgctc tcctcctact tcaccctccg gtctttccaa
     4981 tggtatttcc caaatgcgcc gaatgcgccg gcgatcacgg tatacagaat atgaaaaggc
     5041 tgacagaaaa taaaatattt catgaggggc ctttgctcaa agaagccggc gacccgggaa
     5101 acaaaaaaga attcggcaaa gcacttaagc accaggtaga agaccagcca atgccaacgc
     5161 ggataataaa aactcaaaag gaataaaagc aggaggcagg cattcagcgc ataaaccagg
     5221 gcaagcgtga gaaaaataaa cggatgcccg taatgggaga ccttgcttgc ccagcggatc
     5281 cgctgccgga gaaaggatcg cagatccgct gcgggccgcg tgctcacgat cgctttgggg
     5341 tctttgagaa aggccgccct tccgggaaac cgggactgga tcttccccat gagcagcata
     5401 tcgtcgccgg aagcgatatg atcgatgccg ctgaagccac ccacttccgt gaaggcccgg
     5461 cgcggataag ccaggttcgc cccgttacac atcgggtaaa gatctttata aacggcagct
     5521 cctgtgatgc cctgcaaaga aataaaatcc agcgcctgga atacggagag agccgatccc
     5581 gttgtttcta ttttcaccgg ggcagccagg atctgacaat gccatccggt atacgcactt
     5641 accagggtct ccagccattc cggtcgcgcg atgcaatcgg catccgtgca agcgatcagt
     5701 tcccccgagc tctgtgcgat cccggtctcg atggcctttt tcttatacgc gttcaaatcg
     5761 tccccgctta caaaatcctt caggtccagg agcttcaccc tgccggaata ttgctccacg
     5821 atggaggcgg tccggtctgt ggagaaatca ttcaccacga tgatctccgt taaggaagaa
     5881 ggataggtct gccggagaat ggtttgcagg cagaggccga tatgcagttc ctcatttctt
     5941 gcgggaataa tgaccgagac ggttacgccc ggaacgggat ccccgttttt tgacggaggg
     6001 atccggtccc atatccagtg ataatataag atcagcaggg agtacagtaa caacagcgca
     6061 ccggtaatgg caatgaaaat aggcatcggg tcaatatcca gttggaaata taagaaaagt
     6121 aaaaagcaaa acccggcagc cggcaatccg cttccccgaa tccttcaaaa aaagaattcc
     6181 tgaaacttgc gcgcttagcc ggaaataact agatttgaat agttgtttaa ctaactatat
     6241 gccaaacaac catttcaaga agggggagtt atacagtttt gttaccggca aggcgtccat
     6301 cgccatcgcc cgccggctgc agaaaaaatt caaccaggcc gggttgaata tcaccattga
     6361 acagtggagc gttttatacc agttatggaa gcaggacgga cagagccagc aggagctgtg
     6421 ccgggccacc ttccgcgata aacccagcat tacgcgcctg ctggacaatc tcgaaaaatt
     6481 acaattggtc aaacgcgtgg cctcttccaa cgaccgccgg ataaaccgga tctgcctcac
     6541 gaagctctct tttaaattac aggagcaaac catgctcctg gcggaagaca ccctgaatga
     6601 agccctggaa agagtgcccg gagaacaggt ggaagtgtgt aaatcggttc tgcagatcgt
     6661 ttacgataat cttaaataaa gaaaataaaa ataacctaag atgagcacag caaccaccaa
     6721 acccgaacag atcaaaggcg gcgaatggct gatccgtgaa tctgccggcc cggatatttt
     6781 tatcccggaa gacttcagcg aggaacaacg catggtgaaa gaaatgtgtg cgtccttcct
     6841 ggatacggaa gtgcttccgt tcatggaccg catcgataag ctggaacccg ggctgatgcc
     6901 ttcgctcctg gtgaaggccg gagaacaggg tctcctgggt gcttccattc cggaaaacct
     6961 gggtggtctg ggaaaagatt tcgtcacctc caccattatc aatgaagggc tcggcggagg
     7021 ttattctttt tccgtagccc tcgccgcgca taccggcatc ggaaccctgc ccatcctgta
     7081 tttcggaacg gaagcgcaga aggaaaaata tattccgaag ctggcaagcg gggaatggaa
     7141 ggcctcttac ggattgaccg agcccaatag cggaagcgat gccctcagtg caaaaacgac
     7201 cgcaacctta acgaaggatg gcaaatatta
//
LOCUS       DASYCU010000105         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1185576, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000105 DASYCU010000000
VERSION     DASYCU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185576"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VGM24_02095"
     CDS             <1..196
                     /locus_tag="VGM24_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154178.1"
                     /translation="FPVNLSQVLFFSRGNRIVEKISDGRMTKTNYQTIEFVFVNNKEI
                     YWYFNSEGDRDEEFSRLLTL"
     gene            complement(263..336)
                     /locus_tag="VGM24_02100"
     tRNA            complement(263..336)
                     /locus_tag="VGM24_02100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            481..1827
                     /locus_tag="VGM24_02105"
     CDS             481..1827
                     /locus_tag="VGM24_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY4154179.1"
                     /translation="MKKFRKLAMLPLAAVIFFTVSGGPYGIEPLIGYAGALSIPLLLV
                     IPLLWDIPCMLTVLELNSMMPVEGGYYQWVKRGLGIRWAFYEGWWTWLYTFVDLAIYP
                     VFFVEYANFFFPGINMYKIPVCLAMIWISAGLNIRGIVPVGKSALVLTAVIMIPFLLL
                     FFTGLIHPAHAAAIGVPDHPVFSSISMALYTIMWNYIGWDNATTYAGEVDRPARSYLF
                     SILLAFSAVYFIYAAVTWLALRSGIPANEFADKGIPFLGAMMGGEWLGSLLSIGGMAS
                     MLGIFVAVLLSVSRVPAVMGEDRLLPKAFTRIHSRYQTPFISIIVCAAVVSLLVLRPL
                     SDLLVIDISLYGAGISLEFIALIRLRRKEAAASRPFRIPLQKRGLVLLFIPPLLVFSI
                     ALGGALLGSGQGFIPALFAVAAICSAQPAWFIVSRRKNFIAHQRDSQAKSDSPVIP"
     gene            complement(1824..2213)
                     /locus_tag="VGM24_02110"
     CDS             complement(1824..2213)
                     /locus_tag="VGM24_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4154180.1"
                     /translation="MDNETEIRQLVENWAQAVRNKDIDGILAHHAENLVMYDVPGPFQ
                     SAGINAYRKTWDLFFKYTKPGVFDIHELNVIADGSVGFVYAKMQCADKSGTPDYIPLD
                     FRLTVGLKKIDGQWTILHEHHSIPATT"
     gene            complement(2236..3231)
                     /locus_tag="VGM24_02115"
     CDS             complement(2236..3231)
                     /locus_tag="VGM24_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY4154181.1"
                     /translation="MKIKIYIITYFLFGLCVFQNAQGQQQAAYPAGIIFDSDMGPDYD
                     DVGAITLLHAFADEGKIKILATMASTRYDHVAAVLNVFNTYFRRSEIPIAVPRQNGLL
                     LRDWQHWTDTLVLKYPHSIKTNAEVPEPVDLYRKILSASADTAISIVTVGFLTNLAAL
                     LKSPPDKYSALDGYDLVKKKVKRLVSMGGKFPSGREFNIDQDAASSIYTYSHWPTDVW
                     FSGFEIGEKIKTGLPLIRRESIRHSPVKDVFRICIPMAAEDSAGRKSWDETAVLVAAN
                     GYSPYYTLQKGRIRIAADGSDSWDESGSGQYRLVEREDPEKVRQVINGLIQHQPQ"
     gene            complement(3276..>3750)
                     /locus_tag="VGM24_02120"
     CDS             complement(3276..>3750)
                     /locus_tag="VGM24_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY4154182.1"
                     /translation="FVPELTEVDKFREVPYSISGYNKFISKSGKPAISPPWGTLNAID
                     LNSGDLEWTIPLGEDDTLKAKGVPQTGTENYGGPVITAGGILFIAATKDAKFRAFNKR
                     NGKLLWETRLPAAGFATPSTYEINGRQYVVIGCGGGKLNTVSGDAFVAFTLPETK"
BASE COUNT          781 a          972 c          911 g         1086 t
ORIGIN      
        1 atttcctgta aatctttcgc aggtattgtt tttcagccgg ggtaaccgga ttgtggaaaa
       61 gatttcagac ggccggatga ccaaaacaaa ttaccagacc atagaatttg tctttgttaa
      121 taacaaggaa atttactggt attttaacag cgaaggggac cgcgatgaag aattttcccg
      181 tctcctgacc ctgtgaaccc ggcaaaaaaa aagaccggca taaaaaatat gtcaatcttt
      241 ctttccatcc gatgcaaacc tgtgactccg ataggattca aacctataac ctcttgatcc
      301 gtagtcaagt gctctattca attgagctac ggagccattt taaaaaatca aatctgcaaa
      361 tgattccccg tgtccggggg cggcaaatat aaggcataaa tttttaagca aaaaacgggc
      421 tctgctcccc gcttctcttt gattttatcg taggttgccg gcgtttatat catttctttc
      481 atgaaaaaat tccggaagct cgccatgctc ccgctggctg ctgttatttt ttttaccgtt
      541 tccggtggtc cctatggtat cgagccgctc attggttatg ccggcgccct gtccattcct
      601 ctcctgctcg tcattccact gctctgggac attccctgca tgcttaccgt gctggaactg
      661 aatagcatga tgcctgtgga aggcggatac taccagtggg tgaaacgcgg tctcggaatc
      721 cgttgggctt tttatgaagg atggtggacc tggctttaca cctttgttga cctcgccatt
      781 tatccggtgt tctttgttga atatgccaac tttttctttc ccggaataaa catgtataag
      841 atcccggttt gcctcgcgat gatctggatc agcgccggtc tcaatatccg cggtatcgtg
      901 ccggtgggta aatccgccct ggtgctgacc gccgtgatca tgatcccctt tctcctgctt
      961 ttcttcacgg gactgataca tccggcgcat gccgcagcga ttggcgttcc ggatcatccg
     1021 gttttttctt ccatcagtat ggcgctatat actatcatgt ggaattatat cgggtgggat
     1081 aacgcgacca cctatgccgg ggaagtggat cgcccggcaa ggtcatacct tttttccatc
     1141 ctgcttgctt tttcggcggt ctattttatt tatgccgcgg taacctggct ggccctccgt
     1201 tccggaattc cggccaatga gtttgcggat aagggaattc cgttcctcgg ggccatgatg
     1261 ggaggcgaat ggctgggcag cctgctttct attggaggaa tggccagtat gctgggcatt
     1321 tttgtagctg ttctgctttc cgtttcccgc gtgcccgcgg tcatgggcga ggaccgtctg
     1381 ctgccaaaag ccttcacccg gattcattcc agatatcaga cgccttttat ttcgatcatc
     1441 gtatgcgctg ccgttgtgag cttgctggtg ctcaggcccc tttccgatct gctggtcatt
     1501 gatatttctc tttacggcgc gggtatttcg ctcgagttta ttgcgctgat ccggctccgg
     1561 aggaaggaag ccgccgcttc aagacctttc agaatcccgc ttcaaaaacg gggattggtt
     1621 ttattattca ttcctcccct gctggttttt tccatcgctc tcggaggcgc cctgctgggc
     1681 tccgggcagg gctttattcc tgcactcttt gccgtagccg ctatctgttc tgcccagccc
     1741 gcctggttca tcgtaagccg gagaaagaat tttattgcgc atcagcggga ttcgcaagca
     1801 aagagcgatt cgccggtcat tccctaggtg gtggccggaa tggaatgatg ttcgtgtaaa
     1861 atggtccact ggccatctat ttttttcaga ccgaccgtta atctgaaatc caggggaata
     1921 taatccggag tgccgctttt gtcggcgcat tgcatttttg catatacaaa gcctacactc
     1981 ccgtctgcaa ttacattaag ttcatgtatg tcaaacaccc cgggtttggt gtatttaaag
     2041 aataggtccc aggttttgcg ataggcgttt atgccggcag actggaaagg gccgggcacg
     2101 tcgtacatta ccaggttctc cgcgtgatga gcgagtatcc cgtcaatatc tttgttgcgg
     2161 actgcttgtg cccaattttc tacgagctgc cggatttcgg tttcgttgtc cataatattt
     2221 tttttcagcc ggtaattact gcggctggtg ctgaataagc ccgttgatca cctgccttac
     2281 tttttccggg tcttctcttt ccacgaggcg gtactgaccg ctgccggact cgtcccagga
     2341 gtctgagccg tctgccgcta tccggatccg gcctttctgt aaagtataat acgggctata
     2401 cccgtttgcg gctaccagca ccgccgtctc gtcccagctt tttcttccgg cgctgtcttc
     2461 tgctgccatg ggtatgcaga tgcggaatac atctttcacc gggctgtgcc ggatagattc
     2521 ccggcggatc agtggcaggc cggtttttat tttttcgccg atctcaaagc cgctgaacca
     2581 gacatcggtc ggccaatggc tataggtata aatggatgag gcggcatcct gatcaatatt
     2641 aaattccctg ccggatggaa attttccacc catactgacc agccgtttca ctttcttttt
     2701 caccaggtca tatccatcca aagcggaata tttatccggc ggcgatttca acagggcggc
     2761 caggttggta agaaatccga ccgttacgat gctgatggcg gtatccgcac tggccgataa
     2821 tattttccgg taaaggtcca ccggttccgg aacttccgca ttggttttta tgctgtgcgg
     2881 atattttaaa accagggtat ccgtccagtg ctgccagtcc ctcagcaaca aaccgttttg
     2941 ccggggaacg gcaatgggga tttcactccg gcgaaaataa gtattaaaga cgttcagcac
     3001 agccgccaca tggtcgtaac gcgtgctggc catggttgcc aggattttta tttttccttc
     3061 atccgcaaag gcatgcagga gggtaatggc gccgacatca tcataatccg ggcccatatc
     3121 cgaatcgaaa atgatccctg ccggataagc ggcctgctgc tgcccctgcg cgttttgaaa
     3181 aacgcaaagg ccaaacagga agtaagtaat tatgtatatt ttaattttca tcgtccatct
     3241 gttcttttgc cggtttcgac tccgcctccg gaatcttatt tcgtttccgg cagcgtaaat
     3301 gccacgaaag catctccgga aacggtattt agtttcccgc ctccgcagcc tatgaccaca
     3361 tattggcgcc cgttgatttc gtaggttgag ggagtcgcga atccggcagc aggcagccgg
     3421 gtctcccaaa gtagtttacc gttgcgctta ttgaatgcac ggaattttgc atcctttgtt
     3481 gctgcgatga ataagatgcc gcccgccgtt atcaccggac ctccataatt ttccgttccg
     3541 gtttgcggaa ctccttttgc tttcagcgtg tcatcttctc ccaacggaat ggtccattcc
     3601 agatccccgc tgttcagatc aatcgcgttt aatgttcccc agggtggcga aatggccggc
     3661 ttgcccgatt ttgaaatgaa tttattgtaa ccggaaatac tgtaggggac ctccctgaat
     3721 ttatccactt ccgttaattc cggcacaaat
//
LOCUS       DASYCU010000106         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2200580, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000106 DASYCU010000000
VERSION     DASYCU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200580"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VGM24_02125"
     CDS             <1..299
                     /locus_tag="VGM24_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154183.1"
                     /translation="PVAGLLLHYFISCIWAACYYFISRNPIRKNFLLLNGLGIAVVVW
                     AVMNLLVIPIAFGRAIQHPLAGALKGIPILFVSIGLPFVFIINPAKGKRKKDAG"
     gene            complement(307..1257)
                     /locus_tag="VGM24_02130"
     CDS             complement(307..1257)
                     /locus_tag="VGM24_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY4154184.1"
                     /translation="MIRLEKIIRNHSVYRFITDRTKKIFLPGFEGASLYDVVIFFKSQ
                     VQKVGLNDRARSIAFSFLMAIPAATIFICTLIPYLPVSKKLTRQLLMLTREVTPNQNT
                     YQLVSNFLNDFLNKPRGGLLSFGFLVALFYASNAMLGLMRSFNKSLTQYARRNFLQNR
                     LIAIRLTLVVILLVIGSIVILVTQDELLRLVFKEFHIRDRSMRGVFKTLRWVVIIPLF
                     YFAIAFIYRHAPAVKKKWKLYSPGTMLATVLMIITTFAFSIWVNKFGNYNKVYGSIGT
                     ILILMIVIYINSMILLIGYELNVSIHSLKHISLERNAAGK"
     gene            complement(1254..2291)
                     /gene="mltG"
                     /locus_tag="VGM24_02135"
     CDS             complement(1254..2291)
                     /gene="mltG"
                     /locus_tag="VGM24_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY4154185.1"
                     /translation="MKKLFLIAIAIVFFAGAVSVYLIFGPGTSFNNPKYDLYIRTGMN
                     FNSLMSNLREEKVISHPAVFSWIAARTGYPEKIKAGKYEIRSGMSLFNLMKMLKNGRQ
                     SPVNLVITKLRTKEDLAAMVGRKLECDSATFLAYLNNNDTLRNYSLDSNTVMTAILPN
                     TYTYFWNTNPTRVFRKMETASRTFWTAARREQAASHQLNPVTAYILASIVEEETTRKE
                     DKPKIASVYLNRLAKGMKLAADPTVKFALRDFELKRVYSKYLMVESPYNTYRNIGLPP
                     GPICTPSRESLEAVLNAPDTRYLYFVAKPDFSGYSNFAETYEEHLQNARSYQKSLDEQ
                     MRIGQESKSNP"
     gene            complement(2367..2678)
                     /gene="secG"
                     /locus_tag="VGM24_02140"
     CDS             complement(2367..2678)
                     /gene="secG"
                     /locus_tag="VGM24_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY4154186.1"
                     /translation="MIFFVILLLLACVFLGLIILIQNPKGGGLAGNVAGFSNQFMGVK
                     QTNNVLEKGTWILAIIVALFCIFSSLIIPKTGSSRVRDVKAAPTSVPSGNIPQAPLPV
                     K"
     gene            complement(2675..>3529)
                     /locus_tag="VGM24_02145"
     CDS             complement(2675..>3529)
                     /locus_tag="VGM24_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154187.1"
                     /translation="EITVNEPYEYAPESGPVANDPPSTINEEYREPEITVNEQYEAVP
                     ESGPVADYPPSTINEEYREPEITVNEQYEAAPESGPVAEHAPSTINEEYREPEITINE
                     QYEEALEAYPIVNQELAGYTEPEAPVAPAAPDFEAEAIPSSADSPALPPFDAKKAESI
                     VFAPYHMIDYFASQGIKLVLEDQPSDSFGKQLKSFTDWLKVMKRLPGQRFVPEKADER
                     ESERIRHFAADSLEERDILTETMAEVLAKQGMYENAIALYQKLSLIYPPKSVYFASRI
                     EQLKASLS"
BASE COUNT          860 a          836 c          890 g          943 t
ORIGIN      
        1 acccggttgc gggtttgctg ctgcattatt ttatttcctg tatctgggcg gcgtgctatt
       61 attttatatc ccggaaccct atccgaaaaa atttcctcct gctgaatggc ctgggtattg
      121 cggtggttgt ctgggccgtc atgaacctcc tcgttatacc catcgccttt ggccgggcca
      181 ttcaacatcc cctggcggga gcgctcaagg gcatccccat tctgtttgtc agcatcggac
      241 ttccatttgt attcattata aatccggcga aagggaaaag aaaaaaagac gccgggtaag
      301 ctatttttat ttcccggccg cattccgttc cagggaaata tgcttcaggg aatggatact
      361 cacattcagc tcatacccga tcagcaggat catcgaattg atataaatga cgatcataag
      421 gatcaggatg gtgccgatgg aaccatatac cttgttataa tttccgaact tgttgaccca
      481 gatggaaaag gcaaaagtcg taatgatcat cagcacggtg gccagcatgg ttcccggcga
      541 atagagcttc cactttttct tcaccgccgg ggcatggcgg tagataaacg cgatggcaaa
      601 ataaaaaaga ggaatgatca ccacccaccg aagggtttta aaaaccccac gcatcgaccg
      661 gtcccggata tggaattctt taaagaccag tcgcaggagt tcgtcctggg taaccaggat
      721 aacaatggat ccgatcacca gcaggatcac taccagggta agccgtatgg cgatcagccg
      781 gttttgcaga aagtttctcc tcgcatactg ggtaagggat ttattgaagg aacgcatcag
      841 ccccagcatc gcattggaag cgtagaaaag ggcgaccagg aatccgaagg aaagtaatcc
      901 gccccgcggt ttattgagaa aatcgttcag aaaattactg accagctgat aggtattctg
      961 gttgggcgta acctcccggg tcagcatcag taattgccgg gttagttttt tggagacggg
     1021 cagataagga atcagcgtgc agataaatat agttgccgca ggaatcgcca tcaggaaact
     1081 gaaagcaatg gaacgggcgc ggtcattcag gcccactttc tgaacctggc tcttaaagaa
     1141 gatcaccaca tcataaagag aagccccttc aaatccgggc aaaaatattt ttttggtcct
     1201 gtccgtgata aaccggtaca cgctgtggtt cctaatgatt ttctcgagcc ggatcatgga
     1261 ttcgatttgg actcctggcc gatacgcatt tgttcatcga gtgatttttg gtatgagcgg
     1321 gcattttgga ggtgttcttc atacgtttcg gcaaaattgg aatacccgga aaaatcgggt
     1381 ttggccacaa aatacagata ccgggtatcc ggcgcattta aaacagcttc caggctttcc
     1441 cgggaagggg tgcaaatcgg accgggaggc aaaccaatgt tccggtaagt attataggga
     1501 gattccacca tcaggtattt tgaataaacc cgtttcagtt caaaatcccg cagggcaaat
     1561 ttcaccgttg gatccgcagc gagcttcatt ccctttgcca gccggttcag ataaacgctg
     1621 gctattttcg gcttatcctc ttttcttgtc gtttcttctt ccacaatgga ggccaggata
     1681 tacgcggtta ccggattaag ctggtgggac gctgcctgtt cccggcgggc tgccgtccag
     1741 aaggtcctgg aagccgtttc cattttcctg aaaacccggg ttgggttcgt gttccagaaa
     1801 taggtatagg tattgggaag gatcgccgtc attaccgtat tactgtccag ggaatagttt
     1861 cgaagcgtat cgttgttgtt gaggtaagct aaaaaagtgg ctgaatcaca ttccagtttt
     1921 cgtccgacca tagcggcgag atcttctttt gtccgcagtt tggtgatcac cagatttacc
     1981 ggcgactggc ggccattctt tagcattttc atcaggttga acaggctcat tccggaccgg
     2041 atctcatatt tgccggcttt tattttttcc ggataacccg ttcttgcggc aatccaacta
     2101 aatacagccg ggtggctgat cactttttct tcccggagat ttgacatcag cgaattgaag
     2161 ttcattcccg tccggatata caaatcgtat tttggattgt taaatgaagt gcccggtccg
     2221 aatattaaat agaccgaaac ggcaccggca aaaaagacga tggcgatcgc gataagaaac
     2281 agctttttca cccgtatttg gatttggctt caaaataaac aaaaaaccct gtctttcgaa
     2341 gacagggccc ttaatattgt taaatgctat tttaccggaa gcggagcctg aggaatattt
     2401 cccgagggaa cagaagttgg agcggctttc acatccctta cccggctgct gcctgtttta
     2461 ggaatgatca ggctggagaa aatacagaac agggccacga tgatcgccag aatccaggtc
     2521 cccttttcca atacgttatt ggtctgttta acccccatga actggttgct gaagccagct
     2581 acattaccgg caagaccgcc acccttcgga ttctggatca gaatgatcag tccgagaaat
     2641 acacaggcca gtaacaaaag gataacgaag aagatcatga taaagaggct tttaattgct
     2701 caattctgga cgcaaaataa acgcttttgg ggggataaat caaacttaat ttctggtaaa
     2761 gggcaatcgc attttcatac attccctgct tggccagcac ctctgccatg gtttccgtga
     2821 ggatatccct ttcttccagg gaatctgctg caaaatgacg aattctttcc gactcccgct
     2881 catctgcttt ttccggaaca aatcgctgcc ccggaagtct tttcatcact ttcagccagt
     2941 cggtgaaact cttgagctgc tttccgaaac tgtccgacgg ctgatcctcg agcaccagtt
     3001 ttattccctg ggatgcaaag taatcgatca tatgataggg cgcgaagacg atcgactcgg
     3061 ccttttttgc atcaaatggg ggcagagccg gcgagtctgc agaggaggga atggcctccg
     3121 cttcgaagtc cggcgcagcg ggggcgacgg gtgcttccgg ctctgtgtat ccggccagct
     3181 cctggtttac gataggatac gcttcaaggg cttcttcgta ttgctcattg attgtaattt
     3241 ccggttcgcg gtactcttcg ttgatggttg atggcgcatg ttctgcgacc ggtccggatt
     3301 cgggggcggc ctcatactgt tcgttgactg taatttccgg ctcccggtac tcctcgttga
     3361 tggttgatgg cggatagtct gcgacgggtc cggattcagg gacggcctca tactgttcgt
     3421 tgactgtaat ttccggctcg cggtattctt cgttgatggt tgatggcgga tcatttgcga
     3481 ccggtccgga ttcgggggcg tattcatagg gctcgttgac tgtaatttc
//
LOCUS       DASYCU010000107         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3941467, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000107 DASYCU010000000
VERSION     DASYCU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3941467"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..847)
                     /gene="panB"
                     /locus_tag="VGM24_02150"
     CDS             complement(23..847)
                     /gene="panB"
                     /locus_tag="VGM24_02150"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY4154188.1"
                     /translation="MHSTQYQMVAKKITTHILQNMKAGGEKISMLTAYDFSFAKLFDR
                     AGIDVILVGDSASNVMAGHETTLPITLDQMIYHASSVIRGVSRSLVIVDLPFGSYQSN
                     SKEALISAIRIMKETGAHGVKLEGGEEVMESVKRILAAGVPVMGHLGLTPQSIYKFGT
                     YSVRAREDAEAAKLKADAKLLQEAGCFAVVLEKIPALLAKEVSENLLIPVIGIGAGKY
                     CDGQVLVMHDLLGINTEFKPRFLRQYLNLHEPITEAVAHYIRDVKSNSFPNEQEQY"
     gene            complement(899..1804)
                     /locus_tag="VGM24_02155"
     CDS             complement(899..1804)
                     /locus_tag="VGM24_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY4154189.1"
                     /translation="MTLGAIDIGSNAARLLITEVTVTEKKAIFEKLNLVRVPLRLGFD
                     VFDKKEISPEKANMFLNCIRSFKYLMDAYGVTHCKAAATSAMRDARNSSEIIESVKEA
                     TGIEIEVISGDDEASFIYENHIAENLNSENAYLYIDVGGGSTELTCFADNRLLFKKSF
                     NIGTIRLIKNQVTEAHWDQMKDFLKKEMKGMSKHLTAIGSGGNINKVFSLSKRKDGKP
                     LSLELLRDYYKELNGFSLEDRMRLYNMKADRADVIVPALQIYVNVMRWIDAAEIYVPK
                     IGLADGLIQALYRELCALKTVTETT"
     gene            complement(1823..>2140)
                     /locus_tag="VGM24_02160"
     CDS             complement(1823..>2140)
                     /locus_tag="VGM24_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154190.1"
                     /translation="GKEKVYISSADWMVRNMDHRVEATCPITDITIRKELKEILNIQL
                     ADNVKARWLDNGLQNKYKKDQSKKVRSQIEIYQYLNQKSIKTPAEPAEQGPAAIPEIL
                     VPI"
BASE COUNT          475 a          526 c          504 g          635 t
ORIGIN      
        1 taatcaatga caggagcaat agttagtatt gctcctgttc gtttggaaaa ctatttgatt
       61 ttacgtcgcg gatataatgt gcgaccgctt ccgtaatggg ttcatgcagg ttcaggtact
      121 gcctcagaaa ccggggtttg aattcggtat tgatacccag caaatcgtgc atgaccaaaa
      181 cctgcccatc gcaatacttg ccggctccga tgccaatcac cgggatgagg aggttttcag
      241 atacctcttt cgctagcagg gccggtattt tttccagaac gacggcaaaa catccggcct
      301 cctgcagcag tttcgcgtct gcttttaatt ttgccgcttc cgcatcctcc cttgcccgta
      361 cgctataggt gccgaattta taaatggact ggggagttaa tcccaggtga cccatcaccg
      421 gaacgcccgc ggccagaatt ctttttaccg attccatcac ctcttctcct ccttcgagct
      481 ttacgccgtg ggcaccggtt tccttcatga tccggatggc ggatatcagg gcttctttgg
      541 aattggattg ataggatccg aagggcagat ccacgatgac cagggagcgg ctaacgcccc
      601 gtataaccga agaggcgtgg tagatcatct ggtccagcgt tataggaagc gtggtttcat
      661 gtccggccat aacgttggag gcggaatctc ccaccaggat cacgtcaatt cccgcgcggt
      721 cgaacaattt ggcgaaggaa aaatcatagg cggtaagcat cgaaatcttt tcccccccgg
      781 ccttcatgtt ttgcagaata tgagtcgtga ttttttttgc taccatctgg tattgggttg
      841 aatgcataaa gataattcca ttcaatctga atgggtggaa ttatctttcc cggtatattc
      901 aggtcgtttc ggtaaccgtt ttcagggcgc agagttcgcg gtaaagagcc tggatcaatc
      961 cgtcggccag gccgattttg ggaacgtaaa tttccgctgc atcgatccag cgcatcacat
     1021 ttacataaat ctgcagggca ggaacgatca catcggcgcg gtcggccttc atattataaa
     1081 gccgcatacg atcttccagg gaaaagccat ttaactcctt atagtagtct cttaataatt
     1141 cgaggctcag cggtttccca tcctttcgtt tcgaaaggga aaatacttta ttgatattgc
     1201 cgccggaacc gatggccgtt aagtgtttgg acattccctt catttctttt ttcaggaaat
     1261 ccttcatctg gtcccaatgc gcctcggtta cctggttctt gatcaggcgg atggtgccga
     1321 tattaaaaga ttttttgaac aggaggcggt tgtcggcaaa acaggtaagc tccgtgctgc
     1381 ctccccccac gtcaatatac agataggcat tttccgagtt gagattttct gcaatatgat
     1441 tttcgtaaat aaaggatgct tcgtcgtcgc cgctgatcac ttcaatttct attccggtgg
     1501 cttcctttac cgattctatg atctccgaac tgttccgcgc atcccgcatc gcggaagtgg
     1561 cggcggcctt gcaatgggtc actccgtatg catccatcag gtatttaaag gaccggatgc
     1621 agttgaggaa catgtttgct ttttcgggag agatttcttt tttatcaaat acgtcaaacc
     1681 ccagccggag gggcacccgc accaggttta atttttcaaa aatcgctttc ttttcggtta
     1741 cggtcacttc ggtgatcagc agcctcgctg cattacttcc gatatctatg gctcccagtg
     1801 tcatttatag tttcatggtt tatcagatgg gcaccagtat ctccggaatg gctgccggtc
     1861 cctgttctgc cggctctgcc ggtgttttta tgcttttctg gttcaggtac tgataaatct
     1921 caatttgcga acggaccttt tttgattgat ctttcttgta tttattttgc aggccgttgt
     1981 cgagccagcg ggctttcaca ttatcggcca gctgaatatt caatatttcc tttaactctt
     2041 tccgaatggt tatatcggta atggggcagg tggcttccac ccggtggtcc atattccgga
     2101 ccatccagtc agccgaagat atataaactt tttctttgcc
//
LOCUS       DASYCU010000108         5136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4811239, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000108 DASYCU010000000
VERSION     DASYCU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4811239"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..826
                     /locus_tag="VGM24_02165"
     CDS             236..826
                     /locus_tag="VGM24_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4154191.1"
                     /translation="MAKIRSRKNSSKKDVIIEKASKLFREKGFGAASMRDLAEHVGVE
                     AASLYNHIRSKSEILQAICFKVANDFISHLNAVQSSPESALKKLELIIRFHIRMMMEQ
                     YEFVYISDHEWRHLPEPYLSNFLNQRRNYRRSLSDLIARGIEKGEMKDIDPFVAVLTI
                     LSAISGIESWHRSRKSISSEALENNMVKYLIDGLKK"
     gene            831..1916
                     /gene="paaE"
                     /locus_tag="VGM24_02170"
     CDS             831..1916
                     /gene="paaE"
                     /locus_tag="VGM24_02170"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEY4154192.1"
                     /translation="MAKHFRSLIIKEVRKETPECISVSFHIPEEWAEEFRFHAGQNIT
                     IRTQIPGEELRRSYSICSSPHENELRIAIKKADGGRFSTYAVDSLKAAQSLEVLAPTG
                     NFQLNLQKENRNHYIAFAAGSGITPVISLIKAALKEEPHSRVTLIYGNRNRNSVIFRE
                     ELENIKNVYPERFQLIHIFSREKMDAAIQEGRIDADKCELIFKQLVPLSPASVFLLCG
                     PSSMIFGVRDWLIQQGVDSHKIHFELFSDPGEKTRALTKTGTAAEISEGQKAAVTIHL
                     DGVSHDFQLPFKGETILEAAIRQGADLPYACKAGVCASCRAKLLEGKITMDQNYALAE
                     EELEDGFILTCQSHPASQKIVIDFDTR"
     gene            2012..2875
                     /locus_tag="VGM24_02175"
     CDS             2012..2875
                     /locus_tag="VGM24_02175"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEY4154193.1"
                     /translation="MKNAISVLLLFMLLDGLSVSAQSRTPQNRPATDRRPPAQRSPAQ
                     RPAQAPRPAVSRSSAPRPEQHQRVQRPAVQRQPVQRPPSNRNLANQRQSANHHYATNR
                     RPAINYRPAGWRPSRPAYTRSPHIYGGHRYYTYHHYYYHPYRPYVYGPAWHPAGVFLG
                     ALAATATIIAIDNMQYRYDQGVYYEPVTGGYQVVPAPVDAVVPSLPSGYATLNLGDTE
                     YYYYGGIFYIMSPNGYQVVEAPAGAIVTTLPEGCEEVQADNITYLKFNNTYFQPIQYN
                     GEDAYEVVEIE"
     gene            2955..3890
                     /gene="paaA"
                     /locus_tag="VGM24_02180"
     CDS             2955..3890
                     /gene="paaA"
                     /locus_tag="VGM24_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEY4154194.1"
                     /translation="MSSNEQIFEEKIRQEVRIEPKDWMPEDYRRLLIRQMSQHAHSEI
                     VGMLPEGNWITRAPSLRRKATLIAKVQDEAGHGLYLYSATETLGITRGETIDQLHSGK
                     AKYSSIFNYPTLSWADIGAIGWLVDGAAIMNQVPLCRASYGPYARAMIRICKEESFHQ
                     RQGFEILLTLSRGSEAQQRMCQDAINRWWWPSVMMFGPADEASPHSQKSMQWKIKRFS
                     NDELRQKFIDVCAEQVKTLGMELPDAGLKWNEETGHYDFGEINWAEFRNVVNGNGPCN
                     KERLEARKKAHEEGAWVRDAAAAHTAKKNHSSLSH"
     gene            3894..4193
                     /gene="paaB"
                     /locus_tag="VGM24_02185"
     CDS             3894..4193
                     /gene="paaB"
                     /locus_tag="VGM24_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEY4154195.1"
                     /translation="MEENSNADGWPLWEVFIRPRQGLDHKHAGSLHAADAQMALENAR
                     DVYTRRNEGISIWVVESRFIHASSPEEAGSFFDPANDKVYRYPTFYSLPDELKNI"
     gene            4204..4980
                     /gene="paaC"
                     /locus_tag="VGM24_02190"
     CDS             4204..4980
                     /gene="paaC"
                     /locus_tag="VGM24_02190"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY4154196.1"
                     /translation="MDPLFNYIIQLADNALILGHRNSEWTGHGPILEQDIALSNIALD
                     LLGQARNLYQLAAKRLNTLSPEKKPVTEDDLAYLRDTTEFKNGVLLEQPNGDWGKTIL
                     RQFYFSQFQLLQYQALLDSKEAGLAAISEKSIKETRYHVKWAGEWVCRLGDGTAESRA
                     RMLNAAEELDPWVPELFGATEYEREAAEAGWGVNPAGLYRPWLESVNKIMEMATLPLY
                     PAEKKEGFGLSGKNGFHSEHLGYILAEMQFLQRAYPGCEW"
     gene            4985..>5136
                     /locus_tag="VGM24_02195"
     CDS             4985..>5136
                     /locus_tag="VGM24_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEY4154197.1"
                     /translation="MEKEKIISLLNEVMDPEIPVLSIADLGILREVEVSGNHVRVIIT
                     PTYSGCP"
BASE COUNT         1357 a         1315 c         1341 g         1123 t
ORIGIN      
        1 caaaaaaact ccggccatgc ataacaatga cttaaaaatg gcaggtatta atctgtttgt
       61 tttcataatt ttttggtttt agatgtgctg gtctgaattt gccgggaccc caaatttacg
      121 gcttttcctg cctttgaaac gggaatccct ttatccgctg gcctttcagg ccaaagggca
      181 gtttcccctg cctcccgttt actaacgaac gttcgttcta tctttgcact ccactatggc
      241 aaagatcaga agcaggaaaa acagcagcaa gaaggacgtg atcatcgaaa aagcatccaa
      301 gctcttccgg gaaaagggat ttggggccgc ctccatgcgc gacctggcag agcacgtggg
      361 cgtggaagca gccagcctgt ataatcatat ccggtccaaa tccgagatcc tgcaggccat
      421 ctgttttaag gtggccaatg attttatttc ccacctgaac gcggtgcaat ccagtcccga
      481 gtccgcactg aaaaaactgg aactcatcat ccgctttcat atccggatga tgatggagca
      541 atatgagttc gtttacattt ccgatcatga atggcggcat ctgccggaac catacctttc
      601 caattttttg aaccagcgga gaaattaccg gagaagttta tctgacctga tcgcccgggg
      661 cattgagaaa ggggagatga aggatatcga tccctttgtg gccgtgctga ccattctgtc
      721 tgccatcagc ggaattgaat cctggcaccg gtcaagaaaa tccatcagca gcgaagcatt
      781 ggaaaataat atggtaaaat acctgattga cgggctaaaa aaataagatt gtggcaaaac
      841 atttccgctc cctgatcata aaggaagttc gcaaagaaac ccccgaatgc atttccgttt
      901 cctttcatat tccggaagaa tgggccgaag agttccgctt ccatgccggc cagaatatta
      961 cgatccggac tcaaataccc ggggaagaat tgcgccggtc ctattcaatc tgcagcagcc
     1021 cgcatgaaaa cgaactccgg atcgccatca aaaaagcgga cggcggaagg ttctccacct
     1081 atgccgttga ttcgcttaaa gcggcgcagt cgctggaggt gctcgcaccc accgggaatt
     1141 ttcaactgaa cctgcagaaa gaaaatagaa atcattatat cgcttttgct gccggcagcg
     1201 gcatcacgcc cgtcatctcg ctgataaagg cggcgctgaa agaagaaccg catagccggg
     1261 tcacccttat ttatggaaac cgcaaccgga attcggtcat tttcagggaa gaactggaga
     1321 atataaaaaa cgtctacccg gaacggtttc agctgatcca tattttcagc agggaaaaaa
     1381 tggatgcagc gatccaggaa ggaagaattg atgccgataa atgcgaattg atcttcaaac
     1441 agctggtgcc tttatcccca gcctctgttt tcctcttatg cggaccttct tccatgatct
     1501 ttggcgtccg ggactggctc atacagcaag gcgtggattc ccataaaata catttcgagc
     1561 tgttttcaga tccgggagag aaaacccgcg ctcttacaaa aaccggaacg gccgcggaaa
     1621 taagcgaagg gcaaaaggcc gcggtgacca ttcatctgga tggggtcagc cacgatttcc
     1681 aacttccgtt taaaggagag acgatattgg aagcggccat aagacagggc gccgatcttc
     1741 cctatgcctg caaggcgggc gtttgcgcca gctgccgggc caagcttttg gaaggcaaaa
     1801 taaccatgga tcagaactat gcgctggcgg aggaagagct ggaggatgga ttcatactga
     1861 cctgccagtc gcatccggct tcccaaaaga tcgttattga ttttgatacg cggtaacccc
     1921 cggccccgta caggaaacta gcacgggatt tgaaataata attagttagt tttaaggaag
     1981 cggctgtttc aggcccgcaa attcagaaat tatgaaaaac gctatttcag ttctcctgct
     2041 ttttatgctg ctggatggtt tgtccgtttc ggcacaaagc cgtactccgc aaaaccgacc
     2101 ggcaacggac cgccgcccac cggcacaacg ttcaccggcg cagcggccgg cacaggcccc
     2161 gcgtcccgca gtttccagat catcagcacc aagaccggag cagcatcagc gggtacaaag
     2221 gccggcggtt cagcgtcaac cggtgcaaag gccgccttcc aaccgcaacc tggcaaatca
     2281 gcggcaatca gctaatcatc attatgcaac gaatcgccgt ccggccataa attaccgtcc
     2341 cgccggttgg aggcccagcc gcccggctta cacccgttct ccgcatatct atggcggtca
     2401 tcgctattac acctatcatc attattacta ccatccttac cgtccttatg tatatgggcc
     2461 ggcctggcat cctgcgggtg tcttcctggg cgccctggcg gcaacagcca ctataatagc
     2521 aattgacaat atgcaatacc gttatgacca gggagtgtat tatgaacccg tgaccggagg
     2581 ttaccaggtg gtaccggccc cggtggacgc cgttgtgccc tcgttgccat ccggatatgc
     2641 cacgctgaat ctgggcgata cggaatacta ttattatgga ggcatctttt atattatgtc
     2701 tccgaatggt taccaggtgg tagaggcgcc tgccggtgca atcgtaacta ccttaccgga
     2761 gggctgtgaa gaggtgcagg ctgacaatat cacctacctt aaatttaata acacctactt
     2821 tcaacccatt caatataacg gggaggatgc ctatgaggtg gtggagatcg aatagtggtg
     2881 agaagcccgg tggctgcacg ttcatatact aacaaccgtt agttttttca gcccaaataa
     2941 agtaattttg gggcatgtca tccaatgaac aaatattcga ggaaaagatc cggcaggaag
     3001 tgcgaataga gccgaaagac tggatgccgg aagactaccg gaggctgctg atccggcaga
     3061 tgtcccagca tgcgcacagc gaaattgtgg gcatgcttcc ggagggaaac tggattaccc
     3121 gggctccttc ccttcgcagg aaagcgacgc tgatcgccaa agtgcaggac gaggccgggc
     3181 acgggctcta tttatattcg gcaacggaaa ccctggggat cacccgcggg gaaaccatcg
     3241 atcagctgca ttccggaaaa gcaaaatatt cttcgatatt taattatcca acccttagct
     3301 gggccgacat cggcgccatc ggctggctcg tggatggtgc ggccatcatg aaccaggttc
     3361 cgctctgccg cgcatcctac gggccttatg cccgcgctat gatccggata tgcaaggaag
     3421 aaagtttcca tcagcggcag ggctttgaaa tcctgctcac gctcagccgg ggatccgaag
     3481 cgcagcaacg catgtgccag gatgccatca accgctggtg gtggccttca gtgatgatgt
     3541 tcggccccgc agacgaagct tccccgcaca gtcagaaaag catgcagtgg aagatcaaac
     3601 ggttcagcaa tgatgaactg cggcaaaaat ttattgacgt ttgtgcggag caggtgaaga
     3661 ccctgggtat ggaactgccg gatgccgggt tgaaatggaa tgaagaaacc gggcattatg
     3721 attttggcga aatcaactgg gcggaattcc ggaatgtggt gaacggaaac ggaccctgca
     3781 ataaagagcg gctcgaagcc aggaaaaaag cacatgaaga aggagcctgg gttcgcgatg
     3841 ccgccgccgc ccacaccgca aaaaaaaatc attcatccct tagccattga tccatggaag
     3901 aaaattcaaa cgctgacggc tggccgctct gggaagtgtt catcaggcca cggcagggcc
     3961 tcgatcataa acatgcgggc agcctgcatg ccgcagatgc gcagatggcc ctggagaacg
     4021 caagggatgt ttatacccgc cgcaacgaag ggatcagcat ctgggtggtg gaaagccggt
     4081 tcattcatgc ttccagcccg gaagaagccg gatccttctt tgatccggcg aacgataagg
     4141 tgtaccggta tcccactttc tattcccttc ccgatgaact gaaaaacatt tgatcccgtt
     4201 ccaatggatc cgcttttcaa ctatatcatt cagctcgccg acaatgcgct catcctgggt
     4261 caccggaaca gtgaatggac gggccacgga cccatactcg agcaggatat tgcgctctcg
     4321 aatattgcgc tcgacctcct cgggcaggca agaaacctgt accagctggc agccaaacgg
     4381 ctgaatactt tatctcccga aaaaaaaccg gtaacggaag acgatctcgc ctatctgcgg
     4441 gatacaacag agttcaagaa cggggttctg ctcgaacaac cgaacggcga ctgggggaaa
     4501 accattctcc gccagtttta tttcagccag tttcaattac tgcagtatca ggcactgctg
     4561 gacagcaaag aagccggact tgccgctatc agtgaaaaat cgataaaaga aacccgttat
     4621 catgtaaaat gggccggtga atgggtatgc cggcttggag acggtacggc ggaaagtcgt
     4681 gcccgcatgc tgaacgcagc ggaggagctg gatccctggg tcccggaatt atttggggcg
     4741 acggaatacg aacgcgaggc agcggaagca ggatggggag tgaatcccgc cgggctttac
     4801 cggccctggc tggaaagtgt gaataagata atggaaatgg cgaccttgcc tttatacccc
     4861 gcagaaaaaa aagaggggtt tggtctatcc gggaaaaacg gatttcattc cgaacacctg
     4921 ggctatattc tggccgaaat gcaatttctc cagagagcct atcccggttg cgaatggtga
     4981 accaatggaa aaagaaaaga tcatatcgtt attaaatgaa gtgatggatc cggagatccc
     5041 cgttctttcc attgccgacc tgggcatact gcgggaggtg gaggtatcgg ggaaccatgt
     5101 cagggtaatc ataaccccca cctatagtgg ttgccc
//
LOCUS       DASYCU010000109         4860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3362951, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000109 DASYCU010000000
VERSION     DASYCU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3362951"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2071
                     /locus_tag="VGM24_02200"
     CDS             <1..2071
                     /locus_tag="VGM24_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154198.1"
                     /translation="KYQRVNFRANINANLSDRLRVGVDLSLSNAIQDKLSSSGDAPGV
                     IRHAMLRPPVLGVYKSPDDPTYSASDPYTDLPFYADNLAANGGTWNGAQNFFEFDMNP
                     IAIVHYTDNTLNTFRTFGNVFAEYSLLRDKSLKFKTNLGVDIAFNHNKDFEQNFGDDD
                     GAGGVYAGMGRNNRPNSLNELRGQTNTFTFSNTLNYQKTLNGKHSINAILGEESISSR
                     YDFISGSRINYDNSTDPFRYLDNGSTTLINNSGTQPNNWNLVSFFGSVTYGYDNRYFL
                     TATAREDGSSRFGPSNKWGFFPSVSGAWVVSQEKFMQNLDWISSLKIRGSWGVSGNQE
                     IPNDAYQTVVTQVGGVVNKIRYGNPDVKWESTTQTNIGLDLGVLNNRITLNADYFNKN
                     TDDILLAVPLPAVSVGVIESTYINSGSVNNKGIELGLRYQNFDHAFKYSVSANGATLK
                     NVVTRLYTFVPNIIDNINNTKTQAGHPINAYFGYQFDGIYQNTAEISKTLFTSTNGVQ
                     PGDIKFKDINGDGQIDANDRTFIGNPVPKFTYAFSFNAEYKHFDFSFLFQGVSGVDRY
                     LDLKQILNYDTRPFNSTTAVLGAWTGEGSTNSTPRLSFNDVGGSKISSIFVEDASYGR
                     LKNIELGYTLNLPGARIKSLRFYVSGQNLITVTKYTGLDPESTSLKDQGTYPQMRAFI
                     FGAKINL"
     gene            2092..3570
                     /locus_tag="VGM24_02205"
     CDS             2092..3570
                     /locus_tag="VGM24_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154199.1"
                     /translation="MKKISIIISILGLLVAAGCKKQLTSKSIGLLTQDQVNATPTITT
                     VETSVKSSYQLLSNTLNILGEWDWAGGLVFQNDIVISDVESDDMEKKWAADGDQPWID
                     DINNFTFTSSNGGPNGLWKYDYEGIKRLNIALVFLTNPDLESITGMSTERKNQLLGEA
                     YFLRSYYYFSLVTNFGDVPLITSSVETYQEAFKVAVRVSKDSVWDKIKSDLATAKGLL
                     PDAKLSSATEPWRVSKGAVIALQAKAALYTQDWNSVLSLVSELETTGFYSLNADYFSS
                     FNTKFTDNEVIFAYDHESQQNPLDGNGICAPLSWGFFAPTTDFMNSFEPNDPRKLFTV
                     DVPNQNVNKLLGTLDGTNKGNDDASNNKVLIRYADVLLWKAEAYLETGDYTNAVAYIN
                     KIRARARTTPTATGGTVPAGSLPDRPASTDEATVKAWLVSERRAELGFENQRMPDLKR
                     WGMAKDVMNAAGKNFQDKHYLYPIPQSEIDASAGTLTQNPGY"
     gene            complement(3572..4183)
                     /locus_tag="VGM24_02210"
     CDS             complement(3572..4183)
                     /locus_tag="VGM24_02210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4154200.1"
                     /translation="MKYALGALFLFFFTGIKAQPTVTEQFQKIGTVQEAQAYIDANAA
                     LKPALLHLTLGKDSSLIDKRLLRQNKGDIFSVGYVTYKVLEASDSIAFRASYIFLDAA
                     TLSPSQIDSLQKLIVQKATAGASFEQLSDQYSMDGNTTHGDTGWFFGPAMLPKEFQDA
                     VQNHKKGDIFFVDVPDKQWHYIVKKTYDDRENKEVTILRANGR"
     gene            4511..>4860
                     /locus_tag="VGM24_02215"
     CDS             4511..>4860
                     /locus_tag="VGM24_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY4154201.1"
                     /translation="MPVKQTNTLIIGASISGLSSAASLQKQEIEYIIIEKQDRIAAPW
                     HNHYDRLHLHTPKAFSNLPYKKFGSAIPRYPDRQQVLDYLEDYRGEFNIRPVFNSEAV
                     SVKKEAGFWITETS"
BASE COUNT         1344 a         1118 c         1184 g         1214 t
ORIGIN      
        1 taaataccag cgggtgaatt tcagggcaaa tatcaatgcc aacctgagcg accgccttcg
       61 ggtgggcgtc gatttatccc tcagcaacgc catccaggat aaactttcat ccagcggcga
      121 tgcgcccgga gtgatccgtc atgccatgtt gcggccaccg gttttagggg tatacaaaag
      181 tccggacgac ccgacttatt ctgccagcga tccgtatacg gacctgcctt tttacgcgga
      241 taacctggcg gcaaacggag gaacctggaa cggcgcgcaa aacttttttg aattcgacat
      301 gaacccgatc gccattgtgc actatacaga taataccctg aatacgttcc ggacctttgg
      361 aaatgtattt gcagagtaca gtctgttaag ggataaatca ttgaaattta agacaaacct
      421 cggggtggat attgccttta accacaataa ggattttgag cagaatttcg gagatgatga
      481 tggcgcgggc ggcgtttacg caggtatggg aagaaataac aggcccaaca gcttaaatga
      541 attgagaggg cagaccaaca cctttacttt cagcaatacg ctgaattatc aaaaaaccct
      601 gaatggcaaa catagtataa acgcgatcct gggggaggaa tccatcagca gccggtatga
      661 tttcatcagc ggttccagaa tcaattatga taactcgacc gatccgttcc gatacctgga
      721 taacggcagt acaaccctca tcaataattc gggaacacaa cccaataact ggaacctggt
      781 ctccttcttt ggatcggtca cttatggcta tgacaaccgg tattttctga ccgccacggc
      841 cagggaagac gggtcctccc gcttcgggcc aagcaataaa tggggattct ttccatctgt
      901 atccggcgcc tgggtggtat cccaggaaaa gtttatgcaa aacctggatt ggatttcaag
      961 cttaaagatc cggggaagct ggggcgtttc gggtaatcag gaaattccaa acgatgctta
     1021 ccagaccgtc gtaacgcagg tgggaggtgt ggtaaacaag atccgctatg gcaatcccga
     1081 tgttaaatgg gaatccacta cacagacgaa tatcggtctg gatttaggtg tgctgaataa
     1141 ccggatcacc ctgaacgccg attacttcaa caaaaatacg gatgacattt tgctggctgt
     1201 tccattgccg gccgtatccg tcggcgtaat tgaaagcacc tatatcaatt caggttcggt
     1261 gaacaataaa ggaattgaac tggggttaag ataccagaat tttgatcatg cttttaaata
     1321 cagtgtcagt gcaaacggag ccaccttaaa gaatgtggtg accaggctct atacttttgt
     1381 gcccaacatc attgacaata tcaataatac caaaacccag gcaggccacc ccatcaatgc
     1441 ctatttcggt taccagttcg acggtattta tcaaaatacc gcggagatca gcaaaaccct
     1501 gtttaccagc accaatgggg tacagcccgg agatatcaag tttaaggaca tcaatggaga
     1561 cggccagata gatgccaatg accggacatt cattggaaac ccggttccga aatttaccta
     1621 tgcgttttca tttaatgcgg agtataagca tttcgatttc tcctttttgt tccagggggt
     1681 aagcggcgtg gaccgttacc tggatctaaa gcagatcctg aattatgata cccggccttt
     1741 taattccacg acggcggttc tcggcgcctg gacgggtgaa ggatctacca acagcacacc
     1801 tcgcctttct tttaatgatg tgggaggcag taaaatctcc agcatattcg ttgaggacgc
     1861 ttcttacggg agattgaaaa atattgaact gggctatacc ttaaatcttc ccggcgcccg
     1921 gattaagagt ttgcgctttt atgtatcggg tcagaacctg atcaccgtca caaaatacac
     1981 cggcttggat ccggagtcga cttctttaaa ggatcaggga acctatccgc aaatgagagc
     2041 atttattttt ggcgccaaaa tcaatctttg ataaccattg aaatgagttt tatgaaaaaa
     2101 atatcaatca tcatcagcat cctgggatta ctggttgcag ccggctgtaa aaaacagctt
     2161 acgtcaaagt ccatcggatt gttgacgcag gaccaggtga atgccacacc gaccattacc
     2221 acggtggaaa cttctgttaa gtcatcctat cagctactgt ccaatacgct gaatatcctg
     2281 ggcgaatggg attgggcggg cggcctggta tttcaaaatg atatcgtgat atccgatgtg
     2341 gaatcggatg atatggaaaa gaaatgggcg gctgacggag atcagccctg gatagacgat
     2401 atcaataact tcacctttac ttcttccaac ggcggcccga atgggttgtg gaaatacgat
     2461 tatgaaggga taaaaaggct gaatattgca ttggttttcc taaccaaccc cgatctggaa
     2521 tccattacgg gaatgagcac ggaaagaaaa aaccagttgt tgggagaagc ctattttctc
     2581 cggtcctatt attatttttc cctggtcacc aatttcgggg atgtacccct gatcaccagt
     2641 tctgtggaaa cgtatcagga agcttttaaa gtggccgtaa gggtgagcaa ggacagcgtt
     2701 tgggataaaa ttaaatcgga tctggccact gcgaaaggtt tgctgccgga tgcgaaactg
     2761 agttcagcaa ccgaaccctg gcgggtttcg aagggagccg taattgccct gcaggccaag
     2821 gctgccttat acactcagga ctggaattcc gttttaagcc tggtgtctga attggaaacc
     2881 accggctttt acagtctgaa cgcagattat tttagcagtt tcaataccaa gttcacggac
     2941 aatgaagtga tatttgccta cgaccacgaa agtcagcaaa atccgctgga cggcaatggc
     3001 atatgcgctc cgctgagctg gggtttcttt gcacccacta ccgattttat gaattctttt
     3061 gagcccaacg atccgcgtaa gctttttacg gtggatgttc caaaccagaa tgtgaacaag
     3121 ctactcggca cgctggatgg gaccaataaa gggaatgacg atgcctccaa caataaagta
     3181 ctgatccgct atgcggatgt cctgttgtgg aaggcggaag cttacctgga aacaggtgat
     3241 tacaccaacg ccgttgcata tataaataaa ataagggcaa gggcaaggac cacgccgacg
     3301 gctacgggtg gaacagttcc tgccggttct ttgccggatc gcccggcctc cactgatgaa
     3361 gcaacggtta aggcctggct ggtatccgaa agaagggcgg aactgggatt cgaaaatcaa
     3421 agaatgccgg atctgaaaag atggggcatg gcaaaagatg taatgaatgc agccgggaag
     3481 aactttcagg acaagcatta tttatatccc attccacaga gtgagatcga tgcttctgcc
     3541 ggcaccttaa cacaaaaccc gggatactag actatcttcc gttggcccgc aggatcgtca
     3601 cttctttgtt ttcgcggtca tcgtaagtct ttttaacgat gtagtgccat tgcttgtccg
     3661 gaacatctac gaaaaagatg tcgccctttt tgtggttctg gacagcatcc tggaattctt
     3721 tgggcagcat ggccggtccg aaaaaccagc cggtgtcccc gtgcgtggtg tttccatcca
     3781 tgctgtattg atcggagagc tgttcgaagg aagcccctgc ggtagccttt tgcacgatca
     3841 gtttttgaag gctgtcgatc tgggacgggg atagcgtagc cgcatccagg aaaatataac
     3901 tcgcccggaa ggcaatgctg tcggatgcct caagcacctt atatgttaca taaccgacgg
     3961 aaaagatatc tcctttattt tgccgtaata gccgcttgtc gatgagggaa ctgtcttttc
     4021 ccagggtcag gtgaagcagg gccggcttaa gcgccgcgtt ggcatcgata tacgcctggg
     4081 cttcctgaac ggttccgatc ttctgaaact gttcggtcac ggtcggctgt gctttgatcc
     4141 cagtgaaaaa gaaaaggaat aaagccccga gagcatattt catatacatt tttgtgaacg
     4201 gacgcattat gagcatagct tatttttcag tttgtacgta ataaaaaccc tttttatgaa
     4261 ggtacatttt ttcatattat ttcaattcat cggatgaacg ggaaaatagg gaggggagac
     4321 ctaaatatga aaataaattg ttaataatat caattccatc cgccgctttc cggtatgaaa
     4381 ccggaagaga ttccatcccc cggatgaata tccggtgcgc ccgacgcggg atccgcgact
     4441 cccggaggag aaattagctt caacttttgt aattttaatc cgtacggtcc cgtaactttt
     4501 aaaagatgtt atgcccgtga agcaaaccaa tacgctgatt atcggagcaa gtatttcagg
     4561 attatcttcc gccgcctctt tgcaaaaaca ggagattgaa tatatcatta tcgaaaaaca
     4621 ggaccggata gccgcaccct ggcataatca ttacgaccgc ctgcatttgc atacccccaa
     4681 agcgttttca aatttgccat ataaaaagtt tggcagcgca ataccccggt atccggaccg
     4741 gcagcaggtg ctggactacc tcgaggatta ccggggggaa ttcaacatcc ggccggtttt
     4801 taatagcgaa gcggtatccg taaaaaaaga agccggcttt tggatcaccg aaaccagcag
//
LOCUS       DASYCU010000110         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3797847, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000110 DASYCU010000000
VERSION     DASYCU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3797847"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VGM24_02220"
     CDS             <1..524
                     /locus_tag="VGM24_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY4154202.1"
                     /translation="GISGINLEDYDLRNRKLFSVDAQSRRIGLVRQTAESMGVPLFIN
                     ARTDVYIRGTNSVTKEEKWEETRARGKTYLEAGADGIYPITLTDKEDIGNLVSALACP
                     INILAIPGIPDLKTLAALGVARLSLGPGFLKIAIRAMKQAALELRNGEGLRSVTENEI
                     SSDYLKKLVSSSR"
     gene            complement(547..927)
                     /locus_tag="VGM24_02225"
     CDS             complement(547..927)
                     /locus_tag="VGM24_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154203.1"
                     /translation="MATLTSLQKTLHITYAVVPIVAGLDKFTNLLTNWSAYLGTNIKS
                     MLPMDPMTFMKVVGIIEIIAGILVIIKPLYGAYLVMVWLICIALVLIIGGNYFDVAVR
                     DLVMAVGAYTFAQITLLTKRPPSI"
     gene            complement(1046..2038)
                     /locus_tag="VGM24_02230"
     CDS             complement(1046..2038)
                     /locus_tag="VGM24_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154204.1"
                     /translation="MAITRWGILGCGRIARKFASDLQYVKDARLVAVGSRTQAKADAF
                     AAEFPATYRHDSYEALAENPEVDVIYIASPHSLHHEHTLLCLDRKKAVLCEKALALNS
                     KQVKEMIEKARKEKVFLMEALWTKFLPHYEKLQQILADGELGGIRSMQVNFGFIPQAP
                     VPARLLDPALGGGTMLDIGIYNVFMTLSVLGRPDEIEAVMTPASTGIDEQCAVLFRYK
                     NGALAQLFSSFSSNLATEADINGNKSRIRLTTRFYEPSASIEFYPSKPDSKKIIPFQK
                     EPGFGYQYEARHVGECLRKGLTESPVMTHADSLLLMETLDRVRQAAGIVYSADL"
     gene            2154..3506
                     /locus_tag="VGM24_02235"
     CDS             2154..3506
                     /locus_tag="VGM24_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY4154205.1"
                     /translation="MLDNIKNTETYKTIHLAVPVIIGELAQMSLHLIDTAMVGAISYK
                     HLAAASLVMNAVNIPFVIGMGMTISVSQMVSMANGRRDAQRVSHYLFNGFILCSVFAV
                     LISLGLISCRRILFHLGQDPEVAELAYPFMLLVGISIIPMLLFMTLKQFTDGLEYTRT
                     AMILSLGALPINAFLNWLFIYGHWGFPRLELAGAGWATLITRTVIFVVLGLIVLKHGT
                     FRKYMIISSRQWKLNRKTFRELLHIGIPSSLQLGVEVSAFAVSGIIIGTISAVAQAAH
                     QIALSCASFTFMVSMGLSQAGSIRVSNAYGRSDWTRIRVIGKTTLVTALIYGSCCALC
                     FILLRRQLPALFNQDPDVVQLAGFLLLFAAVFQISDSTQAIGAGLLRGIKDVKIPTGF
                     VSIAYWAIGLPLGYILAFHFRMGAPGIWLGFIAGLTMASFLLVIRFIRMAGKQQNIYG
                     "
BASE COUNT          842 a          903 c          943 g          941 t
ORIGIN      
        1 cgggcatttc cggaattaac ctggaggact acgatctgcg aaaccggaaa cttttttcgg
       61 tggatgcaca atccagaaga atcgggctgg tcaggcaaac ggccgaatcc atgggagttc
      121 cgctttttat caatgcccgg acggatgtct atataagagg aacaaattcc gttacgaagg
      181 aagaaaaatg ggaagagacc cgcgcccgcg gaaaaaccta tctggaagcg ggtgctgacg
      241 ggatctatcc gatcaccctg acggataagg aggatatagg gaacctggtt tctgctcttg
      301 cctgcccaat aaatattctg gccattcccg gaattcccga tctgaaaaca ctggccgcat
      361 tgggtgtggc caggctcagc ctggggccgg gtttcctgaa aattgccatc cgggcaatga
      421 aacaggccgc gctggaattg agaaatgggg aagggcttcg atcagtaacg gaaaatgaga
      481 tcagcagcga ctacctcaaa aagctggtga gctcttcacg ctgaattcca tcgttcgttt
      541 tcgttatcaa atggagggtg gtcttttcgt aagcaatgtt atctgtgcaa acgtataggc
      601 tccgaccgcc attaccaggt cccggacggc cacgtcgaag taatttccac cgatgattag
      661 taccagggct atgcaaataa gccagaccat caccaggtaa gcgccgtaaa gaggtttaat
      721 aattaccagg atcccggcga tgatttctat tatgccgacg accttcataa aggtcatggg
      781 atccatggga agcatggatt taatattcgt tcccaggtaa gcggaccagt tggtgaggag
      841 attggtgaat ttgtccaatc cggccactat gggcacgaca gcataagtga tatgcaatgt
      901 tttttgcaga ctagtaaggg ttgccataat gatttattta atgaatgatc gggttgtctg
      961 ccaattagag ttggggcagg cggaaaggtt acaaaaaggg gctgtttttc cgcacctgat
     1021 gaaatagtag ccttcggcaa cctggctaga gatctgctga ataaacaatt cccgcagcct
     1081 ggcggactcg gtccagggtt tccataagga gcaaactatc ggcatgcgtc atgaccgggc
     1141 tttccgtcag tcccttccgg aggcattctc ccacgtgccg cgcttcatac tgataaccga
     1201 aaccgggttc tttctgaaaa gggatgatct ttttgctgtc gggctttgaa ggatagaatt
     1261 cgatcgatgc agagggctca taaaaacggg tggtcagccg gatgcggctt ttgtttccgt
     1321 tgatatcggc ctcggtggcg aggttggaag aaaagctcga gaacagctgc gccagcgccc
     1381 cgttcttata tctgaaaagg accgcacatt gttcgtcaat gccggtgctg gccggcgtca
     1441 taaccgcttc gatctcatcc ggtcttccca gaacggaaag ggtcatgaac acattgtata
     1501 tcccgatatc gagcatcgtt ccgccaccca gagcaggatc caataaccgc gcaggaacgg
     1561 gcgcctgggg tataaatccg aaattcacct gcatgctgcg gatcccgccc aactctccgt
     1621 ctgcaaggat ctgctgcagt ttctcatagt ggggaagaaa tttcgtccaa agcgcttcca
     1681 tcagaaaaac cttttccttt cgcgcctttt caatcatttc ctttacctgc ttcgaattca
     1741 gggcaagggc tttttcacaa agcacggcct tttttctgtc caggcacagc agggtatgtt
     1801 catggtgtaa agaatgcgga gaagcgatat agatcacatc cacttccgga ttttcagcca
     1861 gggcctcata actgtcatga cgataggttg ccggaaattc cgctgcaaag gcgtctgcct
     1921 ttgcctgcgt cctggatccg acggcgacca gccgggcatc tttcacatat tgaagatcgg
     1981 aggcgaattt ccgggcgatg cgtccgcaac ctaaaattcc ccagcgcgta atggccatct
     2041 gattaatttt tttacaagtt aactattctt ccaaacaact tattcagatc ggggaatgcc
     2101 ggcccctttc ctgtggtact tattttacat ttgttgtttt ttccgaacga agcatgctgg
     2161 acaatattaa aaacaccgag acttacaaaa cgatccacct cgccgtgccg gtcatcatcg
     2221 gggaactggc gcagatgtca ctccacctca ttgatacggc aatggtcggc gcgatcagtt
     2281 ataagcacct ggcggcggcc tctctggtaa tgaatgccgt taatattcct tttgtaatcg
     2341 gaatgggaat gaccatttcc gtttcccaga tggtttccat ggcaaacggc cgccgggacg
     2401 cccagcgggt atcccattac ctttttaacg gtttcatcct ctgctccgtt tttgctgtcc
     2461 ttatttcact gggtctgatt tcctgccgcc gcattttatt tcacctgggt caggatccgg
     2521 aagtggcgga gctggcttat ccctttatgc tgctggttgg aatttccatt attccgatgt
     2581 tgttgttcat gacgctgaaa caatttacgg acgggctgga atatacccgg acggccatga
     2641 tcctttccct cggggccctg cccataaatg cctttttgaa ctggttattc atctatggcc
     2701 attggggttt tccccggctg gagctggccg gagcgggatg ggcgaccctg atcacccgta
     2761 cggtcatctt cgtggtattg ggcctgatcg tgttaaaaca cggcacgttc cggaaataca
     2821 tgattatcag cagcaggcag tggaaattaa acagaaagac cttccgggaa ctgctccaca
     2881 tcggtatacc cagcagcctg cagctggggg tggaagtaag tgcttttgcc gtttcgggga
     2941 tcattatcgg caccataagc gccgttgccc aggccgctca tcagattgcc ctgagttgcg
     3001 cttcctttac ctttatggtt tccatgggcc tttcgcaggc aggttccatc cgggtgagta
     3061 atgcctacgg aagatccgac tggaccagga tccgggtgat cggaaaaact acgctggtca
     3121 ccgcgcttat ctatgggagt tgttgcgccc tgtgttttat cctgctccgc cggcagttgc
     3181 cggcactctt taaccaggac cccgatgttg ttcaactggc aggctttctt ttgctatttg
     3241 ctgccgtttt tcagatatcg gatagcaccc aggccatcgg ggcaggactt ttgcggggga
     3301 ttaaggatgt aaaaattccg acgggctttg tttcgatcgc ctattgggcc atcggacttc
     3361 ccctggggta tatactggct tttcattttc ggatgggggc ccctggcata tggctcggat
     3421 ttatagccgg cctgaccatg gcctcgtttc tactggtgat ccggtttatc cggatggccg
     3481 gaaaacaaca gaacatttac ggatgaatca aaccacccag caattaagca atctcctgga
     3541 ttcgatgacc acggggagtg gggtcatgcg cagcgatatc tatctcaaaa tgatagatga
     3601 agtgcaggac tatgccatta tcttattgg
//
LOCUS       DASYCU010000111         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2928295, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000111 DASYCU010000000
VERSION     DASYCU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928295"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VGM24_02240"
     CDS             complement(<1..1256)
                     /locus_tag="VGM24_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154206.1"
                     /translation="MQRKNNSRRTFLRNTAGALAAFTIVPRHVLGRGFLAPSDQLTKA
                     IVGVGGMGQNHIAYAGTRVVAICDVDRNHLASTLAKLDKGTKTFSDYRELIQLPEVDI
                     VHIATPPHWHGIISADAVRAGKDVWCEKPMTATIGEGKRVIEAVQQHGRIFRLNTWFR
                     FEDIFYGMGTPVKPIKKLVESGLLGWPLKVTISKHTGFDWKFYWVGKTNLPELPVPPE
                     LDYNTWLGPAPYKPYNPHRVHQTFRGYWDYDGGGLGDMGQHYLDPVQYFLGKDDTSPV
                     SVEVDAPQQHPDAVGTWRRITYTYADGCKIILDGEGSETNAAYIEGPNGKLFPGFRSD
                     IADLEKKLASFPDPAPQVTDFIDAVRNRKKFALNESNGHRSCTLVNLGKAALKLGRSL
                     QFDPVNQVFVNDEGANAQLVKPMREP"
BASE COUNT          334 a          405 c          388 g          390 t
ORIGIN      
        1 cagggttcac gcatcggttt taccagttgt gcgttcgctc cctcatcatt cacaaatacc
       61 tggttaaccg gatcaaattg cagggaacgg ccgagtttta atgcggcttt cccgaggttt
      121 accagggtac aggaacggtg accgttggat tcattcaatg caaacttttt cctgttcctg
      181 acggcatcga taaaatccgt cacctgcggc gccgggtcgg gaaaagaagc gagctttttt
      241 tccagatcgg cgatgtccga acgaaaaccg ggaaacaatt ttccattcgg tccttcaata
      301 taagcggcgt ttgtttcgct gccttctcca tccagaatga ttttacagcc gtcggcatag
      361 gtataggtga tccggcgcca ggtgcccacg gcgtcgggat gctgctgggg cgcatcaacc
      421 tctacggata ccgggctggt atcgtccttt ccaagaaaat attgaacagg atccaggtaa
      481 tgctgaccca tgtctcccaa ccctccgcca tcatagtccc agtatccccg gaaagtctgg
      541 tgtacccggt gcggattata gggcttgtac ggagcgggcc caagccaggt attataatcc
      601 agttccggcg gcaccggaag ctctggcagg ttggtttttc ccacccagta gaacttccag
      661 tcgaacccgg tatgtttgct gatggtcact ttcagaggcc agcccagcaa cccgctttcc
      721 accagtttct taatgggttt gaccggggtt cccataccgt aaaagatatc ttcaaaacga
      781 aaccaggtat tcaaccggaa aatccgcccg tgctgctgca cggcttctat cacgcgtttg
      841 ccttccccga tggttgccgt catgggtttt tcacaccata catcctttcc ggcgcgcacc
      901 gcatccgcag aaataatgcc atgccagtgc ggaggggtgg cgatgtgtac gatatccact
      961 tccggcagct ggatcaactc ccggtaatcc gaaaatgttt ttgttccttt atccagtttg
     1021 gccagcgtgc tggccaggtg gtttcggtcc acgtcgcaga tggcgaccac ccgcgtgccc
     1081 gcataggcaa tatgattctg ccccattccg cctacgccta caatggcttt ggtcagctgg
     1141 tcactcggag ccagaaaacc gcggcccagc acgtggcggg gaacaatcgt aaaggccgcc
     1201 agggcgccgg ctgtatttcg caggaaggtt cgcctggaat tattttttct ttgcatgagg
     1261 ttaaataaat tcagtgctaa aaataagcat cctgctttat ataattgccg gaagctggta
     1321 attatccggc aatgctatct cacctcatta tccgttggtt ttggacaggt cacgggctta
     1381 gccccggtta ttgtcccgga tcaggaatcc gcttatgctt ccgttgattg ggaaaaggag
     1441 taaaaatgca ttaactttgg cggttcatta tccgggggtg cctggttttg acagcgtaga
     1501 tctttgaaag cataagc
//
LOCUS       DASYCU010000112         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1625554, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000112 DASYCU010000000
VERSION     DASYCU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625554"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VGM24_02245"
     CDS             <1..655
                     /locus_tag="VGM24_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY4154207.1"
                     /translation="VGGDTSSSQKGFVISVTAIGEVSPDKFVQRGTAQKGDLLCCSGD
                     LGAAYLGLVFLEREKKIFLENPGVKPDLEGEKYVIGRMLKPEAAKEVIDFFEKEEIIP
                     TAMMDISDGLSSEILHICKKSNLGCVVYEEKIPIAEETRIAAYKFELDPTACALSGGE
                     DYELLFTIKQTDFDKLVLNEKISVIGYMTAPDDGKKIITRGGNQFDITAQGWNAFTK"
     gene            733..2190
                     /locus_tag="VGM24_02250"
     CDS             733..2190
                     /locus_tag="VGM24_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY4154208.1"
                     /translation="MSCASSHKTYSPDKKYSVRTLQADYKIFRSILEAAHPSLYWYRS
                     KDSMDYYFNEGYAALKDSMTEDQFRTGLSYVIAKINCGHTSIKASKAYTRYIDTAEEK
                     TFPFIVKFWDDSMVVAANLWRQDPVFRRGTILKSINGYDAEQLTDTLFNYITTDGFSQ
                     SGKYQTLSTGFTFANLYKHVIGLSDSMNVRYLDSLGNERQAYVRLYDFRSDTLRKATK
                     GEAPDEKQTKSKPPKLIYFSSVNLQLDTGMKTAYMTVATFDHNNHLRKFFRNSFKEIK
                     KDHITDLVIDVRSNGGGDAGISTLLTRYLINRKFRLADSLYTVRAPSKYNKYIEKDFW
                     YNMLTHFVTRKESDGKYHFRYFERHAFSPKEQDHFDGQVYLLTGGNSFSATTLFVGAL
                     KGQKNVTVVGEETGGGFYGNTAWIIQDVTLPNTKIRFRLPRFRMVIDRNRGKDGRGIM
                     PDVWALPSSEAIRGGIDYKAMKVKELIDLRKLQSN"
     gene            2213..>2538
                     /locus_tag="VGM24_02255"
     CDS             2213..>2538
                     /locus_tag="VGM24_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154209.1"
                     /translation="MKYFLILLFVLATGRATAQDSLLQARLKSLKGVVMPFSAIAKKD
                     SLFLVCFWATTSDESVNELNAINANLEKWKSALAFRMLGISEDEGKMLNRVRPTYNMN
                     SWTFEV"
BASE COUNT          749 a          552 c          614 g          623 t
ORIGIN      
        1 ggtgggcggc gacacttctt cttcacaaaa agggtttgtt atttccgtca cggcaattgg
       61 cgaagtgagt ccggataaat ttgttcagcg gggtacagcg caaaaaggcg acctgctctg
      121 ctgcagcggt gacctgggcg ccgcttacct gggacttgtt tttcttgagc gtgaaaaaaa
      181 gatattcctc gaaaaccccg gagtaaaacc ggatctcgaa ggagagaaat atgtcattgg
      241 ccgaatgttg aaacccgaag ctgcaaaaga ggtgattgat ttttttgaaa aggaagagat
      301 cattccgacg gcgatgatgg acatcagtga cggactgagt tcggagattc ttcacatctg
      361 caaaaaaagc aatctcggct gtgttgtcta tgaagaaaaa atccccatcg cagaagaaac
      421 ccgcatcgct gcgtataaat ttgaacttga tccgacagcc tgcgcactca gcggaggaga
      481 agattacgag ttgttgttta ccattaagca gacagatttt gataaactgg ttttaaatga
      541 aaagatcagc gtgatcggat atatgaccgc tccggacgac ggaaagaaga ttattaccag
      601 gggcggaaac caatttgata tcacagctca gggctggaat gcgtttacaa aatgaatatt
      661 ttccgttccc cgatgaaaat aggcccgatc ataaaattta tttggctccc cgggatcata
      721 tgcatggctt tcatgtcctg cgcttcttcc cacaaaacct attctccgga taaaaaatat
      781 tcggtccgga ccctgcaggc cgattacaaa atattccgca gcatcctgga agccgcccat
      841 cccagccttt actggtacag gtcaaaagac agcatggact actatttcaa tgaaggatat
      901 gctgcattaa aggattccat gactgaggat cagttcagaa ccggattaag ttatgtgatc
      961 gcgaaaataa attgtggcca caccagcata aaagcatcca aagcctatac ccggtacata
     1021 gacacggccg aggaaaagac cttccccttt atcgttaaat tctgggacga ttccatggtg
     1081 gttgcagcca atctctggcg gcaggatccg gtattcagga ggggcacaat tttaaaaagc
     1141 atcaacggat atgatgcgga gcagttaacc gatacgctct tcaattacat cacaacagat
     1201 ggatttagtc aatccggcaa atatcaaacc ctcagtacgg gttttacttt tgccaacctt
     1261 tacaagcacg taatcgggtt atcggattct atgaatgtcc ggtacctgga tagcctggga
     1321 aacgaaaggc aggcctatgt aagattgtat gatttcaggt ccgatacgtt gcgtaaagca
     1381 acaaagggag aagccccgga tgaaaagcag acgaaaagca aaccacccaa actcatttat
     1441 ttctcttcgg taaacctgca gctggatacc gggatgaaga ctgcttacat gactgtggcc
     1501 acttttgacc acaacaatca cctgagaaag ttcttcagaa attcgtttaa ggaaataaaa
     1561 aaagaccata tcaccgatct ggtgattgac gtccggtcca atggcggcgg agatgcaggc
     1621 atttcgacct tacttacccg gtatctgatt aaccgcaaat tcaggctggc cgactcattg
     1681 tataccgtca gagcgccatc aaaatataat aaatacatcg aaaaggactt ctggtataac
     1741 atgctgaccc atttcgttac gcggaaggag tcggatggaa aatatcattt ccgttatttt
     1801 gaaagacacg cattttctcc gaaagaacag gatcatttcg acggacaggt ttatctgttg
     1861 accggaggca attccttttc cgcaactact ttatttgttg gggcgctgaa gggtcaaaag
     1921 aatgtgaccg ttgtcgggga agaaacgggt ggggggtttt atggcaatac cgcatggatc
     1981 attcaggacg taactttgcc caacacgaag atccggttcc ggcttccgcg tttccggatg
     2041 gtgatagaca ggaaccgggg aaaggatgga aggggaatca tgccggatgt ttgggccctg
     2101 cccagttccg aagcaattcg tggaggaata gattataagg cgatgaaagt gaaagaactg
     2161 attgatctgc ggaaactgca gagcaactaa tccttcatct aaatcaaaga agatgaaata
     2221 ttttctgatt ctgttattcg tattggcaac cggccgggca acagcccagg attccctttt
     2281 gcaggcgaga ttaaaatcgc tcaaaggagt ggtcatgccg ttttcggcga tagcaaaaaa
     2341 agattccctt ttcctggtct gcttctgggc caccacttcc gatgaaagcg tgaatgaact
     2401 caacgccatc aatgccaatc ttgaaaaatg gaaatcagcg ctggcattcc ggatgctcgg
     2461 catatctgag gatgaaggga aaatgctaaa cagggtgagg ccaacctata atatgaattc
     2521 ctggaccttt gaagtttt
//
LOCUS       DASYCU010000113         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1914428, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000113 DASYCU010000000
VERSION     DASYCU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914428"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VGM24_02260"
     CDS             <1..1007
                     /locus_tag="VGM24_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4154210.1"
                     /translation="PFSGYFSSNSATWPAAMKTGNLTIRPFSIVTDVLYDKDKRRATG
                     VRILDAETMKTEEYYARVIFLNASTINTAFILLNSVSDAFPEGFGNSSGQLGHHLMDH
                     HLGVGASGHIEGNEDGYYYGRRPTVAYIPQFRNIDENTRHPDFVRGFSYGGGAGRSGP
                     HRHIDGVPIGAGLKEALSEPGSWSFDLSAMGETLPYFENKVSLDRGKKDKWGLPLLAI
                     DCELKGNEKKMRLDMRSTAAEMLDKVGAKNISTWDSAEQEGQRIFSVHDMGIARMGRD
                     PKTSVLNEHNQMHDVKNVFITDGSCMASSSCLEPSLTYMALTARGCDFAVKELKKGDL
                     "
     gene            1145..2524
                     /locus_tag="VGM24_02265"
     CDS             1145..2524
                     /locus_tag="VGM24_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY4154211.1"
                     /translation="MVKINPDLKPAALHGKLQRFWALSAEKIILLEKNYDVSRGAPVF
                     TVKGAYTSRGWTEWTQGFQYGSAILQYDATGDRSFLEPARRETVARMAPHLSHTGVHD
                     HGFNNVSTFGNLLRLMQEGKLDADEWEKNFYALALKVSAGVQASRWTEIKNGGFIHSF
                     NGPHSLFVDTIRSVRSLVLGHCLGHILQAEGDRRISLLERAVQHLKATAQYSVFYGEF
                     RDVYDVWGRTAHEAIFNTKDGNFRCVNSQQGYSGFSTWTRGLGWAICGFAEQLEWFDT
                     VDEAELNAFSGKKEIISFLLRAARAVCDFYLSESPADGVPYWDTGAPGLTELGDYLDR
                     PADPFNGFEPVDSSAAAIAAQGLLRLGRYLQQQQETEAGDRYWQAGLTVLNALFDAPY
                     LSTDPAHQGLLLHSIYHRPNGWDYIPEGSKIPCGESCMWGDYHIREAALYLQRIINNE
                     PYHTFFHIQ"
     gene            2521..3354
                     /locus_tag="VGM24_02270"
     CDS             2521..3354
                     /locus_tag="VGM24_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY4154212.1"
                     /translation="MNSKENKDLSRLCIHSITTRPWDIEEAAKNFSAAGVKGITVWRD
                     ALNGRNIRQTGKMLRERDLTVVSLCRGGFFADKNGDKRKSAIDDNRRAIEEAAELGTT
                     MLVLVCGADPAQSLEESRKQIRDGIESLIPEASAAGVKLTIEPLHPMYADSRSAINTL
                     AQANDLAEALNSPFVGIAVDVYHLWWDPDLEKEIRRCGENGNLMAFHICDWKLPTRDL
                     LLDRGLMGEGCIPVRQIRSWVEDAGFGGFNEVEIFSNSYWEKNQAEFLNEIIEAYQRH
                     A"
     gene            3392..>3925
                     /locus_tag="VGM24_02275"
     CDS             3392..>3925
                     /locus_tag="VGM24_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154213.1"
                     /translation="MKEHKIGIILNGVTGRMGTNQHLMRSIAEIIRQGGVKINAGETI
                     MPDPVLVGRDENKLKALCSLSGVKKMSTDLDEVLADPHNTIYFDAQTTGRRADAVRKA
                     VKAGKHIYCEKPIAVDTTQAMELYQLCKNSGVKNGVVQDKLWLPGMIKLKRLMQQGFF
                     GRILSVRGEFGYWVFEGD"
BASE COUNT         1037 a          884 c         1089 g          915 t
ORIGIN      
        1 gcccgttttc cggttacttc agcagcaatt ccgccacttg gcctgcagcg atgaaaacag
       61 ggaacctgac cattcgcccc ttctccattg taacggatgt gctgtacgac aaagacaaaa
      121 gaagagcgac cggcgtacgt atcctggatg ctgaaactat gaaaacagaa gagtattatg
      181 cccgggtaat atttctgaat gcatcgacca tcaatacggc ctttatctta ttgaattccg
      241 tttctgatgc ctttccggag ggttttggca acagcagcgg tcagctgggt caccatttaa
      301 tggatcatca tctgggtgtg ggcgcgagtg gccatataga gggtaatgaa gacggctact
      361 attatggcag aagacccacc gttgcttata ttccacaatt cagaaatatt gatgaaaata
      421 ccaggcatcc ggatttcgta cgtggcttct cttacggagg gggtgccgga cgcagcggtc
      481 cgcaccgtca tatagacggg gtgccgattg gcgccggcct gaaagaagcg ttatcagaac
      541 cgggctcctg gtccttcgat ttatctgcca tgggtgaaac gcttccttat tttgaaaata
      601 aagtgagcct ggatcgtggc aaaaaagata aatggggttt gcctctgctg gccattgatt
      661 gtgagctaaa gggcaatgaa aaaaaaatgc ggctggatat gcggagtacg gcggccgaaa
      721 tgctggataa ggtcggtgca aaaaatattt ctacctggga tagcgccgaa caggaggggc
      781 aaaggatctt tagcgtgcac gatatgggca tcgccagaat gggccgggac ccgaaaacat
      841 ccgtactgaa tgaacataac cagatgcacg atgtaaagaa tgtttttatt acggatggct
      901 cctgtatggc ttcgtcttcc tgcctggaac catcactcac ctatatggcg ttaacggcca
      961 gaggatgtga ttttgccgtg aaggaactga agaaaggaga cctttgattt cgtgttaagc
     1021 acccttaatt tgcagggaat tgctttatta attaattatt taatggtatt ggattacttt
     1081 taaatgcgga tgggagtggc atatcagggc ggggcgtttt gattatagta aaattcgcaa
     1141 attaatggtt aaaataaatc cggatttaaa gcccgctgcc ctccatggca aactccagag
     1201 attctgggca ctttccgcgg agaaaataat acttcttgaa aagaactacg atgtatccag
     1261 gggcgctccc gtttttacgg ttaaaggagc atatacttcc agaggctgga cggaatggac
     1321 acagggtttt caatatggtt ctgccatttt acaatatgat gccacgggag accggtcgtt
     1381 cctggaaccg gcgcgcagag agacggttgc ccggatggct ccgcatctca gccataccgg
     1441 cgtgcatgat catggattta ataacgtgag cacatttggc aatttgctcc ggctgatgca
     1501 ggagggaaaa ctggacgcgg atgaatggga aaaaaatttt tatgcgctgg cattgaaagt
     1561 ttccgccggc gttcaggcca gccgctggac ggaaattaaa aacggaggat tcattcattc
     1621 attcaacggg ccgcattccc tgtttgtgga taccatccgg tccgtccggt ccctggttct
     1681 cggtcattgc ctgggacata tcctgcaggc cgaaggagat cgccggatca gcttactgga
     1741 gcgggccgtt cagcacctga aagcgacggc gcagtattcc gttttctatg gcgagttccg
     1801 ggatgtttat gatgtctggg gccgtaccgc acacgaagcg atcttcaata caaaagacgg
     1861 aaatttccgt tgtgtcaatt cccagcaggg ttattccggt ttcagcacct ggacaagagg
     1921 attgggatgg gcaatctgcg gatttgccga acagctcgaa tggtttgata ctgttgacga
     1981 agcagaactc aacgcttttt cgggaaagaa agagatcatt tccttcctgc tcagggcagc
     2041 ccgggccgtt tgtgattttt acctgtctga atcaccggca gacggtgtgc cttattggga
     2101 taccggagct cccggcctta ccgaactggg ggattacctg gacaggcccg cggatccgtt
     2161 caacggcttc gaacccgttg acagttcggc tgctgccatt gctgcacagg gtttgttaag
     2221 actgggaaga tatcttcagc aacagcagga aaccgaagca ggagatcgtt actggcaggc
     2281 gggattaacg gttctgaatg ctcttttcga tgcgccctat ctcagtacgg atccggccca
     2341 ccagggattg ttgctgcatt ccatttatca ccggcccaat ggctgggatt atatcccgga
     2401 gggaagcaaa attccctgcg gcgaatcctg tatgtggggg gattatcata tccgcgaagc
     2461 agccctctac cttcaacgga taataaacaa tgaaccatat cataccttct tccatattca
     2521 atgaattcaa aagaaaataa agatctttcc cgcctgtgca ttcacagcat cacgaccagg
     2581 ccctgggata ttgaagaggc cgcaaaaaac ttttctgcgg ccggtgttaa aggaatcacc
     2641 gtatggcgcg atgcgctgaa cggaagaaat atccggcaaa caggaaaaat gctgagggaa
     2701 cgggatctga cggtggtatc tctttgccgg ggaggatttt ttgcggataa gaacggggat
     2761 aaaagaaaat cggcaatcga tgataaccgg agggccattg aagaagcggc agaactggga
     2821 accaccatgc tggtgctggt atgcggcgcc gacccggcgc agtccctgga ggagtcccgg
     2881 aagcagatcc gtgatggaat cgaatccctg atcccggagg cgtcggcggc gggagtgaag
     2941 ctgacgatag aacccctgca ccccatgtat gccgatagcc gttcggcgat caataccctg
     3001 gcccaggcca atgatctggc tgaagcactg aattcgccgt tcgtggggat cgccgtagac
     3061 gtgtatcatc tctggtggga tcccgatctg gaaaaagaga tccggcgttg cggggagaat
     3121 ggaaacctga tggcatttca tatctgcgac tggaaactgc ctacccggga cctgctgctc
     3181 gaccggggac tgatggggga aggttgtatc ccggtccggc agatccggtc atgggtggag
     3241 gatgcgggat ttggcggatt taacgaggtt gaaatttttt ccaattctta ttgggaaaaa
     3301 aatcaagcgg agtttctgaa tgaaattatt gaagcgtatc agcggcacgc ctgaacaaga
     3361 cattaaaaaa caaaaccggg acaaataaaa tatgaaagag cataaaatcg gcatcatcct
     3421 gaatggcgtt accggccgca tgggtacgaa ccagcactta atgagatcca tcgccgaaat
     3481 aatcaggcag ggcggagtaa aaataaatgc aggcgaaacc attatgcctg acccggtatt
     3541 ggtaggccgg gatgaaaata aattaaaagc attgtgttcc ctgtcgggag taaaaaagat
     3601 gagcaccgat ctcgacgagg tcttggccga tccccacaat acgatatact tcgacgccca
     3661 aacgaccggc aggcgggcag acgccgtccg gaaagcagta aaagcgggta aacatatcta
     3721 ctgtgaaaag cccattgccg tggatacaac gcaggcaatg gaattatacc agctctgtaa
     3781 aaacagcggg gtgaaaaacg gggtcgtgca ggataagctc tggcttccgg gaatgatcaa
     3841 attaaaaagg ctgatgcagc agggattttt tggccgcata ctttcggtgc ggggagagtt
     3901 cggctattgg gtatttgaag gggac
//
LOCUS       DASYCU010000114         3673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_901218, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000114 DASYCU010000000
VERSION     DASYCU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901218"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGM24_02280"
     CDS             <1..211
                     /locus_tag="VGM24_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154214.1"
                     /translation="MNWIMSRKNVNDPGLNYYIGLEGALPVKELIPQKGNGVLVLTAS
                     YTDHGSKDLPNHNRKGRDIVVISVK"
     gene            complement(258..1091)
                     /locus_tag="VGM24_02285"
     CDS             complement(258..1091)
                     /locus_tag="VGM24_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HEY4154215.1"
                     /translation="MVKMGEYNILKLIKEKPMGVFLDDGESGILMPRRFVPAGARVGD
                     ELEVFLYHDGEGRPIATTQKPFGVLGDIVLLKAVSVTPQGAFLDWGLMKDLFIPKSAM
                     LSFIQPMGEYLVKIVMDEQTGRLAATEKLDYFLSNENLTVKELEEVELLVYRRTDIGY
                     LVIINNRHTGVLHFNEVFRNIQTGDRFPGFIKKISAENKIDVVAGKPGYKRVEDETQK
                     LLRLLRENDGYLPYHDKSSPEDIYAFFGMSKKTFKMTTGNLYKQHKIQFTKTGIRLTG
                     E"
     gene            1212..2810
                     /locus_tag="VGM24_02290"
     CDS             1212..2810
                     /locus_tag="VGM24_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154216.1"
                     /translation="MKEEILPDDKSPNTPEMNRISAPGSNARINRRKFVEVAASGALA
                     FTIVPRHVLGGKDFVAPSDKITLAYIGVGTQGIRELLPLLTMQQIQVTAVCDPNKEAR
                     GYRDWSKDGLRNEIRKTIKNPDWNSGGDNLIPGGRENGKSIVDSFYANVRPEQKFKGC
                     TAYADFRELLDKEKDLDAVKVMTPDHLHGLIATAALKKNKHVLMHKPLSNRVLEGKNL
                     IELARSSKKITHLVPWDSNGSMETVMNWINGGSIGTLLEVHDWTNRPVWPQYTTLPVD
                     TPPVPEGLDWNLWLGPEADRPYSPNYTNMVFRSWYDFGGGSMADMGYYSLWTVFNALQ
                     LENPVEIEPNLSHHTDFKDSVPFRIHNDFSFPTSSTVRFKFPAKGSRPPVELCWYDGG
                     MRPACPAELAAENKELPIEGMMFVGDKGKILAGFNVQDPRLLGPKGMEGTGNAKAGRP
                     AQEEKMETSLQLFIDACKSGKQYPGSFAEADHIMEAINLYAVALRSGHLLKYDAAARQ
                     ISNFPEANKYLSREYRTGWDPATI"
     gene            2839..>3673
                     /locus_tag="VGM24_02295"
     CDS             2839..>3673
                     /locus_tag="VGM24_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY4154217.1"
                     /translation="MHPLNRRKFIGKAAMGLGSAWALSQIPAKLLAESGSVFNNRPIG
                     FQTYPIREMIAKDFSGTLKMMAGMGYQLVEMCSPPGYVSSGFGPLVNMKPADMRRIIN
                     DAGLHCPSCHFTFGELTDKLDDRIEFARQLGLTQMICSSFWLPAGAGMKDYLDSVDKL
                     NKIAEQIKKAGLETGFHNHDMEFRKLDGELIYDTLMSHFDPDLVKMQFQTEVINLGYK
                     AADYFKKYPGRFISSHLSDWTAEKKQVPIGQGIIDWKEFFAAAKTGGVKNFFVEMDLD
                     TF"
BASE COUNT          995 a          882 c          899 g          897 t
ORIGIN      
        1 gatgaactgg atcatgagca ggaaaaatgt caatgacccg ggcttaaatt attacatcgg
       61 attggaaggc gccttaccgg ttaaagaact gattccgcaa aaaggcaacg gagtactggt
      121 attaacggcc agttacaccg atcatggatc aaaagattta ccaaatcata accggaaggg
      181 ccgggatatc gtcgttatat ccgtcaaata aatcccttgc cgcaggcttc tatcaaaaat
      241 acccagacgg cctgccccta ttctcccgtt agccggatac ccgtttttgt aaactgtatt
      301 ttgtgctgct tatataaatt tccggtagtc attttgaacg tttttttgct cattccaaag
      361 aatgcataaa tatcctccgg tgaggacttg tcgtgatatg gaagataacc atcattctcc
      421 cggagcaacc tcagaagctt ctgcgtctca tcttccaccc gtttatatcc cggcttaccg
      481 gcaaccacgt ctattttatt ttcagctgat atctttttga tgaagccggg aaaccggtcg
      541 ccggtctgaa tgttccggaa cacttcatta aaatgcaaaa caccggtatg ccggttattg
      601 atgatgacca gataaccgat gtccgtacgg cgatatacca gtaactccac ttcctccagc
      661 tcctttaccg taaggttttc attggagaga aaataatcga gcttctccgt tgccgcgagt
      721 cgtccggttt gctcatccat cacaattttt acgaggtatt cccccatggg ctgaataaaa
      781 ctcagcatcg ccgacttggg aataaaaaga tccttcatca ggccccagtc caggaaagcg
      841 ccctggggag tgacgcttac ggctttcagc aggacaatat cgcctaatac gccaaagggt
      901 ttttgggtgg ttgcgatggg tctgccttcc ccgtcatgat ataaaaaaac ttccagttca
      961 tctcccaccc gggcgcccgc cggaacaaaa cgcctgggca tcaatatccc cgattcgcca
     1021 tcatccagaa aaacgcccat tggtttttcc ttgatcagct ttaatatatt gtattcgccc
     1081 attttcacca tagcggcaga cctctgttat aggcgaagtt agcttccgga aaggatcctc
     1141 ccgccaatta atttttgtca tacaaatttt tatcggtaaa ttttattcct ctaacgatta
     1201 atacaaacat tatgaaagaa gaaattctcc ccgatgataa atctcccaac accccggaga
     1261 tgaataggat atccgccccc ggcagcaatg cccggatcaa tcgccggaaa tttgtggaag
     1321 tggccgcttc cggcgcgctg gcctttacga tcgttcccag gcatgtgctg ggtggaaaag
     1381 actttgtcgc ccccagtgac aaaatcacac ttgcctatat cggagtggga acgcagggaa
     1441 tccgggaatt gctgcccctg ctgacgatgc agcaaattca ggtaaccgcc gtttgtgatc
     1501 cgaacaaaga ggcgcgggga tacagagact ggtcaaagga tggcctccga aatgaaatcc
     1561 ggaagaccat taaaaatccg gattggaatt caggaggtga caatttaatt ccgggcggta
     1621 gagaaaacgg aaaatcaatc gttgacagct tctatgccaa tgttcgtccg gaacaaaaat
     1681 tcaagggctg cacggcctat gcggatttcc gcgaactgct cgataaggaa aaagacctgg
     1741 acgccgttaa ggtgatgact ccggaccatc ttcatggcct gatcgccacc gcggcgctga
     1801 aaaagaacaa gcatgtcctg atgcacaagc cgctttccaa ccgggtattg gaaggaaaga
     1861 atttgattga gctggcgaga agcagcaaga agatcaccca tttggttccc tgggattcaa
     1921 acggatctat ggaaacggtg atgaactgga taaacggggg ttcgatcgga acacttctgg
     1981 aagtgcatga ctggaccaat cgtccggttt ggcctcaata cactaccctg ccggtcgata
     2041 cgcctcccgt tccggaaggc ctggactgga atttatggct cggtccggaa gctgaccgtc
     2101 cctatagccc gaactatacc aatatggtgt tcaggagttg gtatgatttc ggtggcggat
     2161 caatggccga catgggttat tacagtcttt ggaccgtttt taatgccctg caactggaaa
     2221 acccggtgga aattgaaccg aacttaagtc atcataccga tttcaaagat tccgtaccgt
     2281 tccggataca taacgatttt tcctttccga cttcctccac cgtgcgattt aaatttccgg
     2341 cgaaagggtc cagacctccc gttgaattgt gctggtatga cggcgggatg cggcctgcct
     2401 gtcctgcgga attggcagca gaaaataaag agctgccgat agaaggaatg atgttcgtcg
     2461 gcgacaaagg aaagatcctt gccggcttca atgtgcagga cccgaggctg ctcggcccca
     2521 aaggcatgga gggaaccgga aatgcaaaag ccggaaggcc cgcccaggag gaaaagatgg
     2581 agacgtcttt acagttattc atcgatgcct gcaaaagcgg gaaacaatac ccgggcagtt
     2641 ttgcagaagc agaccatatc atggaagcga taaatttata tgctgtcgcc ctgagatccg
     2701 gtcatttgct gaaatacgac gccgctgccc gccagatcag caattttccg gaggccaata
     2761 aatatctgtc ccgggaatac cggaccggat gggaccctgc gaccatctga aaaacagttc
     2821 atcttttaaa aattgatcat gcatccgtta aacagaagaa aatttatagg gaaagccgcc
     2881 atgggcctgg gatctgcctg ggccttgtcc cagataccgg ctaaactgct ggccgaatcg
     2941 ggttcggtat tcaataaccg gcccatcgga ttccagacct atcctatccg ggaaatgatc
     3001 gcaaaggatt tttcaggcac actgaaaatg atggccggca tgggatatca gctggtcgaa
     3061 atgtgctctc ctccagggta tgtgagttcc ggattcggcc cgctggtgaa tatgaaacct
     3121 gccgacatgc gccggatcat aaatgatgcg ggcctccatt gcccaagctg tcattttacg
     3181 ttcggggaac ttacggataa actggacgac cggatagagt ttgcccggca actgggcctt
     3241 acgcaaatga tttgttccag tttctggctg cctgccggcg ccggaatgaa ggactatctg
     3301 gattcggtgg ataaactaaa taaaattgcc gaacagatca aaaaagcagg cttggaaacc
     3361 ggttttcaca atcacgatat ggaattcagg aaactggatg gcgagttaat atacgataca
     3421 ctgatgagtc atttcgatcc ggacctggta aagatgcagt ttcaaactga agtgataaat
     3481 cttggttata aagccgcaga ttattttaag aaatatccgg gaaggtttat ttcctcccat
     3541 ttatcggatt ggactgcgga aaagaaacag gtgccgatcg gccagggaat cattgactgg
     3601 aaggaatttt ttgcagcggc caaaacgggt ggcgtgaaga atttctttgt tgaaatggat
     3661 ttggacacgt tta
//
LOCUS       DASYCU010000115         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2784692, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000115 DASYCU010000000
VERSION     DASYCU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2784692"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..543
                     /gene="rpsK"
                     /locus_tag="VGM24_02300"
     CDS             133..543
                     /gene="rpsK"
                     /locus_tag="VGM24_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY4154218.1"
                     /translation="MAKAQQQQTSAKAAAKKRVVKVDAYGDAHVSASFNNIIISITNK
                     NGQLISWSSAGKMGFKGSKKNTPYAAQMASADAAKTALDAGLKRVDVYVKGPGSGRES
                     AIRSLANNGIEVVMIKDVTPLPHNGCRPPKKRRV"
     gene            664..1269
                     /gene="rpsD"
                     /locus_tag="VGM24_02305"
     CDS             664..1269
                     /gene="rpsD"
                     /locus_tag="VGM24_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY4154219.1"
                     /translation="MARYTGPKTKISRMFGEPILGNGKWLNKNSNPPGQHGATRKRKT
                     LGEYALQLKEKQKAKYTYGVLERQFRKTFEEASRRKGVTGENLIRLLEARLDNTIYRL
                     GIAPSRPGARQLVSHKHITVNGEVVNIPSFQLKPGDSIGLKPSSQGNAGITGLAKGKG
                     NKINWLDWNEAEMKGTFIAYPERDSVPENIKEQLIVELYSK"
     gene            1307..>1794
                     /locus_tag="VGM24_02310"
     CDS             1307..>1794
                     /locus_tag="VGM24_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY4154220.1"
                     /translation="MAILNFQKPDKIILQKATDFEAQFEFRPLEPGYGVTIGNALRRV
                     LLNSLEGYAIIGVKIEGADHEFATLKGVTEDVVEIILNLKQVRFKKKVDHEVAQEKIT
                     LSLKNKTEFTAATIGEVLPSFEVMNPGLQICTMDASARLDIEITIGKGRGYVPAEDNK
                     PK"
BASE COUNT          549 a          417 c          459 g          369 t
ORIGIN      
        1 acacggaaag gaaaacgcag aacggtcgct ggcaaaaaga aggctcctaa aaagtaaaca
       61 gtccgtatcc tgaaggggat ggggattata tcatagcact attttcagaa atcagattac
      121 ctagttcgaa caatggcaaa agcacagcaa cagcaaacca gcgctaaagc cgctgcaaaa
      181 aaaagggtcg ttaaagtgga tgcttacggg gatgcacatg tatctgccag ctttaacaat
      241 atcatcatca gcataaccaa caagaacggc cagctcattt cctggagcag tgccggcaag
      301 atgggattca aggggtctaa aaaaaatacc ccgtatgctg cgcagatggc gtctgccgat
      361 gcggccaaaa cggcactgga tgcaggtctg aaaagagtgg acgtatatgt gaagggcccg
      421 ggatcaggaa gggaaagcgc gatccgctcg ctggccaata acggcattga agtggtaatg
      481 atcaaggacg tgacgcccct gccgcacaat ggctgccgtc cgcctaaaaa gagaagggtt
      541 tagtatttcc ccggcaggct ggtaaaaaat cagcttccgg tatttataat caagctgggc
      601 aggattccat ttttaagatt tttcataatc ctgtccgggt actcaaaaat ctgagattta
      661 tttatggcac gttacaccgg tccgaagacc aagatctccc gcatgtttgg ggagcctatt
      721 ttaggtaatg gaaaatggct gaataaaaac agcaatcccc ccggacagca tggggcaacc
      781 cgcaagcgta aaacccttgg cgaatatgcg ctccagctaa aagaaaagca aaaggccaag
      841 tacacctacg gggtgcttga gcgccagttt cgcaaaacgt ttgaagaagc ttcccgacgc
      901 aaaggggtga ccggtgaaaa cctgatccgt ttgctggagg cgcggctgga caataccatt
      961 taccggctgg gcattgcccc atcccgtccc ggtgcccgcc agctcgttag ccataaacat
     1021 attacggtta acggggaggt ggttaatatc ccttctttcc aactgaaacc cggagacagc
     1081 attggcctga aaccttcatc ccagggaaat gccggcatca ccggtctggc caaaggaaag
     1141 ggaaataaaa tcaactggct ggactggaat gaagcggaaa tgaaaggcac attcattgcc
     1201 tatccggaaa gggatagtgt tccggaaaat atcaaggaac aactgattgt ggagttgtac
     1261 agcaagtaag cgtattcatc attaataaat aatcgaaaat ttaaatatgg ccattttaaa
     1321 tttccagaaa ccagacaaaa tcattcttca aaaagcaact gatttcgaag cccagttcga
     1381 attccgtcct ttggagcccg gttacggggt aaccatcggt aatgcccttc gccgggtatt
     1441 gctgaattcc ctggaaggat atgcgatcat cggcgtaaag attgaaggag ctgatcacga
     1501 attcgccacc ctcaaggggg ttacggaaga tgtggtggaa atcattctga acctgaaaca
     1561 ggttcggttc aagaagaagg tggatcatga agtggcacag gaaaaaatca cgctcagcct
     1621 caagaacaaa accgaattca ccgcggccac catcggagaa gtattaccca gtttcgaggt
     1681 gatgaacccc ggattgcaga tctgcacgat ggatgcctcc gccaggctgg acattgagat
     1741 caccatcggc aaaggccgcg ggtacgttcc ggcggaagac aacaaaccca agga
//
LOCUS       DASYCU010000116         5517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1769440, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000116 DASYCU010000000
VERSION     DASYCU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769440"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VGM24_02315"
     CDS             complement(<1..954)
                     /locus_tag="VGM24_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4154221.1"
                     /translation="MPNLERTDESPEKPGNYRWVVCSLLFFATTINYLDRQVMGWLKP
                     VLAEKFHWTETDYSHIVMAFTAAYALGLLVFGRVIDKISSKLSYTFSVTIWSVAAMLH
                     AAVKTTFGFGLARAMLGIGESGNFPAAVKTVAEWFPKKERALATGFFDSGSNIGALLV
                     PLFIPWILSRYGWQEAFIITGALGFIWLIAWRLFYEIPEKQKRLLPAELEYIKSDHSP
                     GEKTKEAGPGIRWIQLFGLRQTWAFIVGKFFTDPIWYFFLFWLPSYFSNTYDMDLKKP
                     SLPMILVYSGATIGAIGGGYLSSRLIKKGWPVFRARKLALLV"
     gene            complement(947..2254)
                     /gene="gluP"
                     /locus_tag="VGM24_02320"
     CDS             complement(947..2254)
                     /gene="gluP"
                     /locus_tag="VGM24_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HEY4154222.1"
                     /translation="MMSLSAALLMEPSDQRQYRKNITIIGVFFFIFGFITWINATLIP
                     YLRIACELKEWEAYLVTFAFYISYTVMAIPSSRLLQRTGMIKGMSIGLAVMGLGCLIF
                     IPAAVTRQYSLFLTGLVFAGGGLTLLQTAVNPYITFLGPENSAAKRISIMGICNKTAG
                     ILAPWIMSNIILKDSGGLLDQLKQMNAEEKKARLDALSQTVITPYICLSAILFLIALL
                     IRYAHLPELKTSSGPQTSTGENKPLGKAVRTQYILGFTAIFFAVGCEVMSGDTIGNYG
                     LYHGINLDISKHLASYPLTAMMIGYITGVVCIPKYISRQKAFSFSCRLGLFITPLIVI
                     LPGRFSIACVALLGLANAMLWPAIWPQALKGLQGKALAKGSAILIMAIAGGALMPLTY
                     GWLSTIINNQYAYLLLLPAYLYLLYYSGVAKREMNLSQKTSNA"
     gene            complement(2300..3100)
                     /locus_tag="VGM24_02325"
     CDS             complement(2300..3100)
                     /locus_tag="VGM24_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEY4154223.1"
                     /translation="MTESEAGKLKVRIYENRNLLGTDAAEMAATRINELLETKDMVNI
                     IFAAAASQNEFLDAISRKQVNWERVNAFHMDEYIGLSEDAPQQFGKWLKERIFGKLPF
                     HRIYYLNGQATDLPAECARYGELLEQHPPDLTFMGIGENAHLAFNDPHVADFRDPRLV
                     KVVDLDEACKQQQVNEQCFGTISQVPAYALTLSIPALLKAAYIYCMVPGASKSEAVYC
                     TLNAGITERCPSTILRTHPNAWLFLDWESASRILEEKKDQPPAITKNV"
     gene            complement(3277..>5517)
                     /locus_tag="VGM24_02330"
     CDS             complement(3277..>5517)
                     /locus_tag="VGM24_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4154224.1"
                     /translation="EQIMSTQENGQRSVDLDGIPVGLLASMEVVKTLTPDLDGDAIAG
                     VVNLQTPKATSTHTKLSADMGLGYNNLRSSLNGLGNLSVGKRFLVDDKNPNGKLGIML
                     NGSYYRTKNGYDELNAQVWQQKDFGKGDIYFPTDVRYLFVQNERTRKGASATVDYNFN
                     STSSLVANVMYSDYYTDILRYRKRTRMQSANTTQNGTGDFVTAKGRSYNEVKSENTDV
                     RNLNFSLEGETKVGRVKLDGAVFATNADYRDNTGTYNFITGNIPLSIHDIGTDNLLAT
                     GTDWRNDASLFTYNTVEHESYKNNGKNFVARANMTYPYKIGNNSAIFKAGFKIKNTHS
                     QRYYPDNVVISTYAGDKAVGKLTNFEGKQEVSSKLMDGTVDFGLGVDKDKTINFYNSD
                     QGPDFPLDNSSTRETIDTYFYDAVENITAGYLMNRIQFNRLMLLAGLRVESTDVNYKG
                     NIVDEDADGLWMSTTPNQKKTNYTKFLPNLQAKYDLNRSTVLRGGLTFNYARPDFVEL
                     VPGRIISKLAETVVDGNPDLQPAFATNVDLALEKYLSNLGILSINGFYKNISDFQYSS
                     VTILQGNEFENASPYVGYQWFRTLNGNTAKVFGIELNAQTNLSFLPGFLKGFSVLANY
                     TYTHSNADAQFRKDIRLPGQATNTANASLAYTIKGFTIQGNLNYNGSYTVSLGADAAT
                     DVIRDGRLQLDANASYRISKRFTVYAEALNLTNAPQVDYFGERSRIYTKQYYSFWGRA
                     GIKFRL"
BASE COUNT         1313 a         1402 c         1410 g         1392 t
ORIGIN      
        1 aaccagcagg gccaatttgc gggccctgaa tacgggccat cctttcttga tcagccggga
       61 ggataaataa cccccgccga tggccccgat ggttgccccg gaatacacga ggatcatcgg
      121 aaggcttggc tttttgagat ccatatcata ggtattggaa aaataggaag gcagccagaa
      181 aagaaaaaaa taccagatgg gatcggtaaa gaatttacca acgatgaacg cccaggtctg
      241 acgcaggccg aacagttgaa tccaccggat gcccggacca gcttccttcg ttttttcacc
      301 gggagaatga tcgcttttta tgtattccag ttcggccggg aggaggcgtt tttgtttctc
      361 cggtatctca taaaaaagac gccaggcgat cagccagata aatcccagcg cgccggtaat
      421 gataaaggct tcctgccagc cgtagcggct cagtatccag ggaataaaca ggggaaccag
      481 taaggcgccg atattggagc ccgagtcgaa gaagccggtg gccagcgctc tttctttttt
      541 cggaaaccat tctgccacgg tcttgaccgc cgcgggaaaa ttaccggatt caccgatgcc
      601 cagcatggcc ctggccaggc caaagccgaa cgtggtcttt accgccgcat gcaacatggc
      661 cgccacgctc cagatcgtga cggaaaaagt ataactcagt ttgctgctga tcttatcgat
      721 aacccgcccg aatacgagca ggcccagcgc ataggcggcc gtaaaagcca tcacgatatg
      781 actgtagtcc gtttccgtcc agtgaaattt ttccgccaga accggcttca gccaacccat
      841 cacctggcgg tccaggtaat tgatcgtggt agcaaaaaaa agcaggctgc aaacgaccca
      901 gcggtaatta cccggtttct cgggggattc atccgttctc tccagattag gcattgctcg
      961 ttttttgtga aagattcatt tcccttttgg ccacgccgct gtaatacaaa agatagaggt
     1021 aagcgggaag caggagcaga taagcatatt gattattgat gatggtcgac agccagccat
     1081 aggtcagcgg catcagcgcg ccgccggcaa tggccatgat caggatggcc gaaccctttg
     1141 ccagcgcctt accctggagg ccttttaacg cctgcggcca gatggcgggc cataacatgg
     1201 cattggccaa tcccaacagc gccacgcagg ctatggaaaa cctcccgggc agaataacaa
     1261 tcagcggagt gatgaataac ccgagccggc aggaaaaaga aaaagccttt tggcgggata
     1321 tgtatttcgg aatgcacacg acgccggtaa tataaccgat catcatagcc gttaacggat
     1381 aggaagcaag atgcttcgaa atatccaggt ttatgccatg atatagccca taatttccga
     1441 tggtatcgcc agacatcact tcgcagccga cggcaaaaaa aatggccgtg aacccaagta
     1501 tatattgtgt ccggaccgcc tttcccaacg gcttgttttc gcctgtggaa gtctgcggac
     1561 cggaggaagt tttcagttcc ggcaggtgcg cataccggat cagcagggca atgagaaaca
     1621 ggatcgcgga aagacagata taaggtgtga tcaccgtctg cgacaaggca tcgagccgcg
     1681 cttttttctc ctccgcattc atctgcttca actggtccag caatccgccg gagtccttta
     1741 agatgatatt ggacatgatc cagggcgcaa ggattcctgc cgtcttattg cagataccca
     1801 tgatgctgat ccgttttgcc gcagaatttt ccgggccgag aaaagtgatg tagggattga
     1861 ccgctgtctg caaaagagta aggccgccac ccgcaaaaac caatcccgtc aggaacagac
     1921 tgtactgcct cgttaccgcc gcggggataa atatcagaca gccgaggccc atgaccgcta
     1981 acccgatgct catgcctttg atcatcccgg tccgctgcag caaccggctg gagggaatgg
     2041 ccatgacagt gtaagaaata taaaaggcga aagtgaccag gtaggcttcc cactctttca
     2101 actcgcaggc gatccgcagg taaggaatca gggtggcatt gatccaggta atgaaaccga
     2161 agatgaaaaa gaacaccccg atgatggtaa tgttctttct gtattgtctt tgatcggatg
     2221 gttccatcaa gagtgctgca gataatgaca tcattttttg tttaaccaaa tgtaggcatc
     2281 tctgggttta cccgcatgtt catacgtttt ttgtgatcgc cgggggttga tctttctttt
     2341 cctctaaaat acgcgaagca ctttcccaat caaggaacag ccaggcattc gggtgcgtgc
     2401 gaaggatggt tgaagggcac cgttcggtga taccggcgtt caaggtacaa tacaccgcct
     2461 ctgacttact ggccccgggc accatgcaat agatatacgc cgccttcagc agggcgggaa
     2521 tgctcagggt cagcgcatac gcaggaacct ggctgatcgt accaaaacac tgctcgttca
     2581 cctgttgttg cttgcacgct tcgtccaggt caactacttt caccagccgg ggatccctga
     2641 aatcggcaac atggggatca ttgaaggcaa gatgtgcatt ttcgccgata cccataaaag
     2701 ttaaatccgg gggatgctgt tccagtaatt cgccgtaccg ggcgcattcg gccgggagat
     2761 cggtcgcctg cccgttgaga taatagatcc ggtgaaaagg aagtttcccg aatattcttt
     2821 ctttcagcca tttaccgaat tgttgcgggg cgtcttcgct caacccgata tattcatcca
     2881 tatgaaatgc atttacccgt tcccagttta cctgtttccg gcttattgca tccaggaatt
     2941 cattctgcga cgccgccgcc gcaaaaatga tattgaccat gtctttcgtt tccagcagtt
     3001 catttattct ggtggccgcc atctctgctg catctgtccc gagcaggttc ctgttttcat
     3061 agatccttac tttcaacttg cctgcttctg attctgtcat ggttaattct gttggggttt
     3121 ccgtttccgg taatggcgtc cggcaaaccg gtggctgttg cagaaaatag tttctaaaga
     3181 gaatagctgc aaccggccac cgtttgcgga cggcttcgga ggtaaatcat aggcagaaag
     3241 attaaagccg ggtccgatat tccggctttg acgcgtttac aatctgaatt taataccggc
     3301 gcgtccccag aaagaataat attgtttggt gtagatcctg gaacgttctc caaaataatc
     3361 tacctgcggc gcattggtca gattgagcgc ttccgcatac acggtgaacc gtttgctgat
     3421 gcggtaagaa gcattggcgt ccagttgcag ccttccgtcc cggataacat cggtagcggc
     3481 atcggcaccg agactaacgg tataagaccc gttataattc agattgccct ggatggtaaa
     3541 tcctttgatg gtataggcaa gcgacgcatt cgccgtattg gtggcctgtc ccggcagccg
     3601 gatgtctttt ctgaattgcg catccgcatt ggaatgtgta taggtatagt tggccaggac
     3661 ggaaaatccc ttcaggaaac ccggaaggaa gctgaggttg gtctgcgcat tcaattcaat
     3721 tccgaatacc ttagccgtat tcccgttcag cgtgcggaac cattgatatc ccacataggg
     3781 actggcgttt tcaaattcat ttccctgcag gatcgttacg ctgctatact ggaagtcact
     3841 gatattcttg taaaaaccat tgatggaaag aatacccagg ttggaaagat atttttccag
     3901 ggcgaggtcg acattggtgg caaaagcggg ttgcagatcc ggattaccat ccactacggt
     3961 ctcggcgagt ttgctgataa tacggccggg caccagttca acgaaatcgg gccgtgcata
     4021 attgaaggtg agtccgccac gtaataccgt actccggttg agatcatact tggcctgcag
     4081 gttgggcagg aacttggtat agttggtttt cttctggttc ggcgtggtgg acatccagag
     4141 gccgtctgca tcttcatcca cgatatttcc tttataattc acatcggttg attccacccg
     4201 cagtccggcc agcagcatga gccggttaaa ctggatacgg ttcatcaggt aacccgctgt
     4261 gatattttca acggcgtcat agaaataggt atcgatggtt tcccgcgtgc ttgaattgtc
     4321 aagcggaaag tccggaccct ggtcgctgtt gtaaaaatta atggtcttgt ccttatccac
     4381 gcccaatccg aaatccaccg tgccatccat taatttggaa gacacttcct gcttgccttc
     4441 gaaattggtc agctttccga cggccttgtc gccggcatag gtgctgatga ccacattgtc
     4501 tggatagtaa cgctgactgt gggtattctt gatcttgaac ccggccttga aaatggcgga
     4561 gttgttgccg atcttatagg gataggtcat atttgccctg gccacgaaat tcttaccgtt
     4621 gttcttataa ctttcatgct ccacggtatt gtaggtgaac aggctggcat catttctcca
     4681 gtccgtgcct gtcgccagca ggttatcggt tccgatgtcg tggatggaca gcggaatatt
     4741 gccggtaata aaattatagg tgcccgtgtt atcccggtag tcggcattgg ttgcgaaaac
     4801 agctccgtcc agttttaccc ggccgacctt tgtttcgcct tccaggctga agttgaggtt
     4861 ccgtacgtcc gtattctccg atttcacttc attgtaggac ctccctttgg cggtcacaaa
     4921 atcaccggtt ccattctggg tggtgttggc gctttgcatc cgggtacgct tccggtaacg
     4981 caggatgtcg gtgtaataat cactgtacat cacgttggcc acgaggctgg aggtggaatt
     5041 gaaattataa tccaccgtag ctgacgcgcc cttgcgggtg cgttcattct gcacaaacaa
     5101 atagcgtaca tcggtgggaa aataaatatc tcccttaccg aaatccttct gctgccagac
     5161 ctgggcattc agttcatcat acccgttctt tgtacggtaa taactgccgt ttagcatgat
     5221 gcccagtttg ccgtttggat tcttgtcgtc caccaggaaa cgtttgccga cgctgaggtt
     5281 cccaagtccg ttcagactgc tccggagatt attgtaacca agccccatgt cagcgcttag
     5341 tttggtatgg gtggaagttg cttttggtgt ttgcagattg accactccgg cgatcgcatc
     5401 gccgtcgaga tccggggtaa gcgttttcac cacttccatg gaagcaagca ggcctaccgg
     5461 aattccatcc aggtccacgc tgcgctgtcc gttttcctgc gtgctcatga tctgctc
//
LOCUS       DASYCU010000117         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4088496, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000117 DASYCU010000000
VERSION     DASYCU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4088496"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGM24_02335"
     CDS             <1..268
                     /locus_tag="VGM24_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HEY4154225.1"
                     /translation="ILIGDPKNLDDSPTDATPHVHRIIGILHRKFPTIPVIPVDERHT
                     SMMARRSLLEMGLKKSKRREKGTTDQVAATLMLQEFLQQRDTGA"
     gene            297..872
                     /gene="def"
                     /locus_tag="VGM24_02340"
     CDS             297..872
                     /gene="def"
                     /locus_tag="VGM24_02340"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY4154226.1"
                     /translation="MILPIIAYGAQVLRTRGQEITADYPGLTKLIEDMWETMYESSGV
                     GLAAPQINKSIRLFIVDSTQIFRNLDEADKDKYPDGPGIKRVFINPKILKLNGEPWAY
                     SEGCLSIPKIHEEILRNEEVTLEYDDELFQQEKKTFNGITARIILHEYDHIEGKLFID
                     YLKPLKRKLLRSKLDDISKGKVNVDYKMSFG"
     gene            complement(940..2118)
                     /locus_tag="VGM24_02345"
     CDS             complement(940..2118)
                     /locus_tag="VGM24_02345"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY4154227.1"
                     /translation="MSRKPKEDKQVLQGTPEIAAGSGAAFAPPSGASGENILPPGEPE
                     EGNPEEMPEASDTLYERMNLVVDRGQEPVRLDKFLTARIENISRNKVQQAIDGGRVLV
                     NDKIVSANYKIRPGDNIISYSGRQAQGEEIIPEKMPLNIFFEDEEVLIINKPPGLVVH
                     PASGNYHGTLINGVAWYLQEKNKAISEETLPRFGLVHRIDKNTSGLLVLAKTSRAVAS
                     LARQFFDHTVKRQYVAVVWGDLQNEEGTIVAHVGRHQRFRKQFEAYPDGEHGKEAITH
                     YRVLERFGYVTLVQCVLETGRTHQIRVHMKSIGHPLFNDDTYGGDKIVKGTVFSKYKQ
                     FVENCFAVCKRQALHAKTLGFIHPATKEEKLFVSELPADMEELIGKWRKYTATLKGER
                     "
     gene            2211..2942
                     /gene="lipB"
                     /locus_tag="VGM24_02350"
     CDS             2211..2942
                     /gene="lipB"
                     /locus_tag="VGM24_02350"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY4154228.1"
                     /translation="MREPVLFRDLGKMPYAEAWDYQECLLQENVQVKTRRRSGEKGSE
                     DLSTSNYLLFVEHPPVYTLGKSGHEENVLLNESEMAARGIRLFHTNRGGDITFHGPEQ
                     IVGYPILDLEKFGTDIGKYLRNLEEIIIRTLADYGIRGERSKGETGVWLQPGIPGKER
                     KIAALGVRCSRWITMHGFAFNVNTDLSYFEGIIPCGIRNKQVSSLARELGRKVDLAEV
                     KAHLKIHFGEVFQAVLIQSAIPGQE"
     gene            complement(2979..>3634)
                     /locus_tag="VGM24_02355"
     CDS             complement(2979..>3634)
                     /locus_tag="VGM24_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154229.1"
                     /translation="YMFTKSKKAITEKIVLPKEPPKQPVARAEAAEEKELAIPKIDNS
                     RISYLQDKRNLKNPAVNSPDKIKTPAIQVEKTLFVKKGDSVIMKLAGNKVRQFSDSLL
                     KETRDSLLFVSTDTLLIKPFVEPPVEKKDVYRISPYVFTAKDGNVNIALADWNNKKYS
                     VRFFEDDNTPLIEIKDIKDPLLIIDKTNFLHSGWFRFELYEDGRLKEKNKLFIPKEF"
BASE COUNT          858 a          885 c          930 g          961 t
ORIGIN      
        1 gatcctgatc ggcgatccga agaatctcga tgatagtccg acggatgcca ctccccatgt
       61 tcaccggatc atcggtatcc ttcaccggaa attcccaact attcccgtaa ttcccgtaga
      121 tgaacggcac acgtcgatga tggccaggcg gtccctgctg gaaatgggcc ttaaaaaaag
      181 caaacgcagg gaaaagggaa ccacggatca ggtggcggca accctgatgc tgcaggagtt
      241 tttgcagcag cgggatacgg gagcttaaat gatctgattt tattaaattt gcggctatga
      301 ttttaccgat tatcgcatac ggggcgcagg ttctgagaac ccgggggcag gaaataacgg
      361 ccgattatcc cggtttgacc aaactgatcg aagatatgtg ggaaaccatg tatgaaagca
      421 gcggggtcgg attggccgct ccccagatta ataaatccat ccggcttttt attgtcgaca
      481 gcacccagat ctttcgcaat ctggatgagg cggataaaga taaatatccg gacggacccg
      541 gtattaaacg ggtttttatc aaccctaaaa ttctgaaatt aaacggagaa ccctgggcgt
      601 attcggaagg ttgcctgagt attccaaaga tccatgaaga gatcctccgg aatgaagaag
      661 tgaccctgga atacgatgac gaacttttcc agcaggagaa aaaaacgttc aatgggatta
      721 cagcccgcat catcctgcat gaatacgatc atatcgaagg aaaacttttc atagattacc
      781 tgaaaccact caaacgcaag ctcctgcgca gcaaactcga tgatatctcc aaaggcaaag
      841 tgaatgtgga ttataaaatg agttttggat aagaaatcct ccgcggcccg gtaccagtag
      901 aggaacggca ggctctcgcc aggatacagg gttttgccct taacgttccc cctttagggt
      961 tgccgtatat tttctccact tcccgatcag ttcctccatg tcggcgggca gttcacttac
     1021 aaatagtttt tcttctttgg ttgcggggtg gatgaagccg agggtctttg cgtgcagcgc
     1081 ctgccgtttg cagacagcaa aacaattttc aacaaattgt ttgtacttgc tgaataccgt
     1141 tcctttcaca attttatctc cgccataggt atcgtcgttg aaaagcggat gaccgatgga
     1201 tttcatgtgc acccggatct ggtgggtccg gccggtttcc aatacgcact ggaccagggt
     1261 tacgtatccg aatctttcca agacccggta atgcgtgatg gcttcctttc cgtgttctcc
     1321 atcgggatag gcttcaaatt gttttcggaa gcgctggtgc cggccaacat gtgcaacgat
     1381 ggttccttcc tcgttttgga ggtctcccca aacgaccgcg acatactgac gtttaaccgt
     1441 gtggtcgaag aattgcctgg ccaggctggc taccgcgcgg ctggtctttg ccagcaccag
     1501 caggccgctg gtatttttgt ctatccggtg aacgagtccg aaacgcggca gggtttcctc
     1561 cgatatggct ttgttctttt cctgaagata ccaggccacc ccgttgatca gtgtgccgtg
     1621 gtaatttccc gaggcgggat gaaccaccag tcccggaggt ttgttgatga tcagcacttc
     1681 ctcgtcttca aaaaaaatat tcaacggcat tttttccggg ataatttctt ccccctgggc
     1741 ctgcctgccg gaataactga tgatattgtc gccgggtctg atcttgtaat tggccgaaac
     1801 gattttgtcg ttcaccaata cccttccgcc atcaatcgcc tgctgaactt tattccggga
     1861 aatattttca atccgggcag tgaggaattt atcaagacga accggttcct gacccctgtc
     1921 cacgaccaga ttcattcttt catacagcgt gtccgatgct tccggcattt cttccgggtt
     1981 gccctcctcc ggttctccgg ggggaaggat attttcaccg gaagcgcccg atgggggggc
     2041 aaaggcagca ccggagccgg cggcaatttc cggggtccct tgtaaaacct gtttatcctc
     2101 tttcggcttc ctgctcattc cggcaaaaat actgccttcc gggatgatac ccccaaagtg
     2161 gcctaatgat cgtcccccgg cccttccgcc ggtttcctta attttggagt atgagagagc
     2221 ccgttttatt cagggatctg ggaaaaatgc cgtatgccga ggcctgggat taccaggaat
     2281 gcctgctgca ggaaaatgtg caggtcaaaa ccaggcgcag gtccggggaa aagggatcgg
     2341 aagatctttc caccagcaat tacctgttgt ttgttgagca tccgccggtt tataccctgg
     2401 gcaagagcgg ccacgaagaa aacgtattgc tgaatgaatc cgaaatggct gcccgcggga
     2461 tccggctttt tcataccaac cggggagggg atatcacttt tcacgggccg gaacagatcg
     2521 ttggataccc gatcctggat ctcgaaaaat tcgggacaga tatcggtaaa tacctgcgta
     2581 acctggagga aatcattatc cggaccctgg ctgattacgg gatccgggga gagcgttcaa
     2641 aaggggaaac cggcgtttgg cttcagcccg gaattccggg aaaggaaaga aagatcgcgg
     2701 cgctgggcgt tcgctgcagc cgctggatca ccatgcacgg atttgccttc aatgtcaata
     2761 ccgacctgag ctattttgag ggaatcattc cctgcggcat ccggaataaa caggtgagtt
     2821 cccttgccag ggaactgggc agaaaggttg atcttgctga agtgaaagca catttgaaga
     2881 tccatttcgg cgaagtgttt caggccgttc ttatccagtc cgctattcca gggcaggaat
     2941 aaaaaacagg aatcccgttg ttcgccgggg ggcgccggct aaaattcttt cgggatgaag
     3001 agcttgtttt tttccttgag cctgccgtct tcatacaact caaagcggaa ccagccggaa
     3061 tgtaaaaaat tcgttttgtc gatgatcagc aatggatctt tgatgtcctt tatttcgatc
     3121 aggggggtat tgtcgtcctc aaaaaaccgg accgaatatt ttttattgtt ccagtcggcc
     3181 agggcgatat ttacatttcc gtctttggca gtgaatacat agggggaaat ccggtaaaca
     3241 tctttctttt ccaccggtgg ctcaacaaag ggtttgatca gcagggtgtc tgtactcaca
     3301 aacaacaggc tgtccctggt ttcttttaga agggaatcgc tgaattggcg aaccttattg
     3361 ccggcaagct tcatgatcac cgagtcgccc ttttttacaa acagcgtttt ttccacttga
     3421 atggcaggcg ttttgatttt atccggactg tttacggccg ggtttttcag gttccgttta
     3481 tcctgtaaat aagagatcct ggaattgtcg atttttggaa tggcgagttc tttttcttcc
     3541 gcggcttccg ccctggcaac cggctgtttt ggcggctctt tgggtaatac tattttttcc
     3601 gtaatggctt ttttggactt ggtgaacatg tatt
//
LOCUS       DASYCU010000118         7682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2494585, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000118 DASYCU010000000
VERSION     DASYCU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2494585"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2308)
                     /gene="alaS"
                     /locus_tag="VGM24_02360"
     CDS             complement(<1..2308)
                     /gene="alaS"
                     /locus_tag="VGM24_02360"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY4154230.1"
                     /translation="MMGSAEIREKFLDFFRSKGHVIVPSAPIVVQHDPTLLFTNAGMN
                     QFKDYFLGNKMAPHPRVADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYF
                     KKEAVAWSWELLTGIYGLPADRIYITVFGGDPSENLPADEETRNEWKKWVPQDRILAG
                     SKKDNFWEMGDTGPCGPCTEIHVDCRSEEERRTRDGKTLVNNDHPQVIEIWNNVFIQF
                     NRNRSGTLEPLPLKHVDTGMGFERLVRVLQNRQSNYDTDIFTGTIREVAAITGIPYSG
                     SENGKADIAFRVIADHIRAICFTIADGQLPANTGAGYVIRRILRRAVRYYYSYLGRQE
                     PLLFMLVPRIADQFALVFPELQRQEAFVARVIQEEEESFLRTLEKGLKIFNEYVEGEK
                     TKTKQISGSFAFKLNDTFGFPVDLTSLLAREIGWTVDEAGFEKELQLQKNRSRSATQI
                     DTGDWIPVHENGNGLFDGYAQLETDTEVSRYRKVKIKGKEAYQLILNKTPFYAESGGQ
                     VGDTGSLQFTDGNIAVLDTKKENGLIVHFIEKLPDRMEGPVHAVVDAVKRKNTAIHHS
                     ATHLLHAALRKVLGTHVAQKGSLVNEGHLRFDFSHFAKLKDTELASIEQIVNEKIREN
                     IPVIIREMPREEALRTGAMALFGEKYGDMVRVVIIDPEFSIELCGGTHVTHTGELGFF
                     KIVSESAVAAGVRRIEAVSGIAAERYVSDQFSMLHSIKELLKNPKDLQKSVLDLTAEN
                     SRNRKKAESLEALQLLQKKQDLLKQVIKY"
     gene            2438..3409
                     /locus_tag="VGM24_02365"
     CDS             2438..3409
                     /locus_tag="VGM24_02365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4154231.1"
                     /translation="MKKIKYYYNTNTLRYEKLETPLRVTLLRILGFLSASIVTALILV
                     SLAYRYFPSANEKRLMQANDALKDNYFVLNAQVKKMQEQIGGLEKRDNEVYRAIFEAN
                     PIPDSARAKFLAKEQEIQRVQGMDQDNLASSIVSSLNNLESRIGAQSKSYDDINGFIK
                     NKEQLLACTPAIQPVSNKDLKRIASGFGYRVDPVYKTTKFHAGLDFAAPLGTPIYATA
                     NGTVETAGNTGNGYGNNVVIHNGYGYSTLFGHMFRIKVRAGQKVKRGEIIGWVGSTGK
                     STGPHCHYEVHRNGEPVDPVYYFYNDITPEQYDRLLKLASASNQSFD"
     gene            3467..4057
                     /locus_tag="VGM24_02370"
     CDS             3467..4057
                     /locus_tag="VGM24_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY4154232.1"
                     /translation="MDNLTQISLNFIEEAPLRQDLPVNPEDQPLNIGIRIKKLKQPER
                     MEPVPEPAIPDTKPAEPFLKPKSGRGRKSIRQISEEADLIQIPEDEQLFSKQYYTMGE
                     VSEMFRVNQSLLRFWEAEFSILQPKKNKKGDRYFRPVDIKNLHLIYHLLRQRKYTIEG
                     AKDFLRKNKKAEEKFESIKKLEQVKEFLLEMKAMIR"
     gene            complement(4070..5188)
                     /locus_tag="VGM24_02375"
     CDS             complement(4070..5188)
                     /locus_tag="VGM24_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY4154233.1"
                     /translation="MQDFWNQPILDNPVKSYLLVVLTIVIGLLFKRILSRLIAGQLYR
                     FASTLDSGLDKQSFVKLLLAPVETFLLAFIAIVAIEKLHFPKVLEFEIYEISSRIIIH
                     SAAKTVLILMFIWLLLRIIDFVALLLKKKANLTGGQKDHQMLVFFRDFLKVILVIIGI
                     LMILSISFGFEVSKLWTGLGIAGAALALATKESIENLIASFIIFFDKPFQTGDILKVN
                     NISGTVEKIGLRSTRIRTDQKTYVTVPNKQMVDSIVDNQSLRTQRKGEIRLQLDPSTT
                     SDTIQQLIGECKKILSKTYIDNPTVFLNDISGTAFQLNIDYFTPPISFADFNEIKQKV
                     NLEILGLLEMLRITLSGSSVSVKLMGGNEGKTDKPKKG"
     gene            complement(5192..6001)
                     /locus_tag="VGM24_02380"
     CDS             complement(5192..6001)
                     /locus_tag="VGM24_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154234.1"
                     /translation="MKKLISYIILSWALLSADAQVDSPGLKAHFIPGSFIDFEVDNLG
                     NIYALSTDNRLKKMNAAGDSLAVFNDVRRYGRISYMDVANPLKILLYYQEFGTIVMLD
                     RFLNNINSIDLRKFNLFQVKSIGLAYDNNVWVYDELEGKLKKIGDDGSLILETTDIRQ
                     LTDSIPDPARLTDQGGLVYLYDSLKGVFIFDHYGGFQKFLPLQNWQHFSVIDHTILGW
                     EGHYYLKYTEKTGEQEKQEIPAYFLPAIKMTIRPENVYVLKTDGIHIYKNG"
     gene            complement(5998..6783)
                     /locus_tag="VGM24_02385"
     CDS             complement(5998..6783)
                     /locus_tag="VGM24_02385"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY4154235.1"
                     /translation="MQIAEGKKIYFLSDFHLGSPDRLTSLQREKYIIQFLESIRSRAA
                     EIFILGDLFDFWYEYRTVVPKGYVRILGKLAELTDSGIPIQFFVGNHDMWMNNYFEKE
                     LNIPVYYEPKTFIRNGKKFLIGHGDGLGPGDKGYKFLKRIFRNKYCQWLFGILPPAAG
                     VGLANYFSRKSRVATGLADDRFLGTGGEWLIGYCKNVLDKEFFDYLIFGHRHLPIDFT
                     FPNGSRYINLGDWIRYFTYAEFDGGSLSLQSFDASLNNQIIRK"
     gene            complement(6797..7435)
                     /locus_tag="VGM24_02390"
     CDS             complement(6797..7435)
                     /locus_tag="VGM24_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY4154236.1"
                     /translation="MKVSPAKENILKRIRQALTEPTPIPFPQSEGNSSVYRPSSQEKE
                     IEFAEQLAQVQGKFIYCADQDEMLRQLKLLIAKNAWAQIFCRETALRRILDEPLKGLL
                     TAGGLPEADASITSCELLIARTGSILLSAAQESGRTVSVYAPVHICIAYSNQLVYDIR
                     EGLEFLQDKYKKGLPSLITLASGPSRTADIEKTLVVGVHGPKDVYVFLIDAE"
     gene            complement(7443..>7682)
                     /locus_tag="VGM24_02395"
     CDS             complement(7443..>7682)
                     /locus_tag="VGM24_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154237.1"
                     /translation="RELLTKKNSQLHLLAEALLEKEVLFQSDVETLIGKRPFEEKRAL
                     DVSEHELDDVPELKLPDNGLPAPPVAVPPIAEEPI"
BASE COUNT         1964 a         1938 c         1770 g         2010 t
ORIGIN      
        1 tgtattttat tacctgtttt aacaggtcct gctttttctg gaggagttga agggcttcca
       61 gggattctgc cttttttcta ttccttgaat tttcggcagt aaggtccagc acgctttttt
      121 ggaggtcctt tggattcttt agcaattcct ttatggagtg cagcatcgaa aactgatccg
      181 atacgtaccg ctctgccgca atacccgaaa cggcttcgat ccgtcttacg cccgccgcaa
      241 cagctgattc ggatacaatt ttaaaaaagc ccaattcacc ggtgtgggtc acatgggttc
      301 cgccgcacag ttcgatggaa aactccggat caataatcac gacccggacc atatctccgt
      361 acttttcccc aaaaagcgcc atggcgcccg ttctcagtgc ttcttcgcgt ggcatttccc
      421 ggatgatgac gggtatgttt tcccttatct tttcatttac gatttgttct atggaggcca
      481 gttcggtatc cttcagtttt gcaaaatgag agaaatcaaa acgcagatgc ccttcattca
      541 ccaacgatcc tttttgagcg acatgcgttc ccagcacttt tcgcaaggcg gcatgaagca
      601 ggtgcgtagc cgaatgatga atggcagtgt tctttctttt aacagcgtcc accacggcat
      661 gaacaggccc ttccatgcgg tccggcaatt tctcaataaa atgaacgatg agtccatttt
      721 ctttttttgt gtccagcacg gctatgtttc cgtctgtgaa ctgtaaacta cccgtgtccc
      781 cgacctgtcc tccgctttcc gcataaaatg gggtcttgtt aagtatgagc tgataggctt
      841 cctttccttt gatctttact ttccggtacc ggctcacctc cgtatccgtt tcaagctgag
      901 catatccgtc aaaaaggcca ttcccgtttt cgtgaaccgg aatccagtcg ccggtgtcga
      961 tctgggttgc cgagcgggag cggttttttt ggagctgaag ttctttttca aaaccggctt
     1021 catctaccgt ccagccgatc tcccgggcca gaagcgaagt gagatcgacc ggaaatccaa
     1081 acgtatcgtt cagtttgaaa gcaaaggagc ccgaaatttg tttggttttt gtcttctccc
     1141 cttccacata ttcattaaat attttcaatc ccttctccag ggtcctcaga aaagactctt
     1201 cttcttcctg tattactcgc gccacaaaag cttcctgccg ctgtaattcg ggaaatacca
     1261 gcgcaaactg atccgcaatg cgcgggacca gcataaacag caggggctcc tgccggccga
     1321 gatatgaata ataataacga acggcccggc gaagaatccg cctgatcaca taacctgcac
     1381 cagtattcgc aggcagctga ccatccgcaa tcgtaaaaca gattgcacgg atatgatctg
     1441 cgatcacgcg aaaggcaata tccgccttgc cattttccga tcccgaatac ggtatcccgg
     1501 taatggcggc tacctcccgg atggttccgg tgaaaatatc cgtatcatag ttgctttgcc
     1561 tgttttgcaa aacacgcacg agcctttcaa aacccattcc ggtgtccacg tgttttaaag
     1621 gaaggggttc gagcgtaccg gaccggtttc ggttaaactg aataaataca ttgttccata
     1681 tttcaatgac ctggggatgg tcattgttca ccaaagtctt tccatccctg gttcttcttt
     1741 cttcctctga gcggcagtcc acatggatct ccgtgcaggg cccgcagggt ccggtatctc
     1801 ccatttccca gaaattatcc ttcttgcttc cggcgagaat acggtcctgc ggaacccatt
     1861 ttttccattc gttgcgggtt tcttcatctg caggcaggtt ctccgaagga tctccgccga
     1921 ataccgtgat atagatccgg tcggccggca gcccataaat tccggttagc aattcccagc
     1981 tccaggcaac ggcttccttt ttaaaatagt cgccaaaact ccagttaccc agcatttcaa
     2041 acatggtatg gtggtaagtg tccacgccca cttcctccag atcattgtgt ttcccgctta
     2101 cgcggagaca tttctgcgta tccgccaccc ggggatgggg agccatttta tttcccagaa
     2161 aataatcctt gaactgattc atcccggcat tggtgaaaag cagtgtggga tcgtgttgga
     2221 cgacaatcgg tgcagaagga acaataacat ggcctttcga cctgaaaaaa tcaaggaatt
     2281 tttcccgtat ctcagcgctt cccatcatat tttaacagac tatctttggt tggaggatta
     2341 taaaaggata ataatttgta caattgcgtt attaatcagg cgctgcaaag gtacagcaat
     2401 cattttcgta aaccgactgc gtgtactacc ggactgcatg aaaaagatca aatattatta
     2461 taataccaat accctccggt acgaaaaact tgagactccg ctacgcgtaa ccctcctgcg
     2521 catattgggt ttcctgtctg cctccattgt aactgccctg atcctggttt ccctggcata
     2581 ccgttatttt ccctctgcga acgaaaaacg cctgatgcag gccaatgatg cattgaagga
     2641 caactatttt gtgctgaatg cccaggtaaa gaaaatgcag gagcagatcg gcggacttga
     2701 aaaacgggac aatgaagtat accgggccat ttttgaggcc aatcccatac cggatagtgc
     2761 gcgggcgaaa tttctggcca aagaacagga gattcagcgg gtccagggca tggatcagga
     2821 taacctggcc agctctatag tcagctcttt aaacaacctg gaatcaagga tcggcgccca
     2881 gtccaaatct tatgacgata tcaacgggtt catcaagaac aaagaacagc ttctcgcctg
     2941 tacccccgca atacaaccgg tgagcaataa ggacctgaaa cgcattgcct ccggcttcgg
     3001 ctaccgggtg gatccggttt ataaaacgac caagtttcac gcggggcttg attttgcagc
     3061 ccccctggga acgcctatat atgccacggc caacggaacg gtggaaacgg ccggcaatac
     3121 gggaaatggt tatgggaata atgttgtgat ccataatggg tacgggtatt ccaccttatt
     3181 cggacatatg ttccggatca aggtccgggc cggccaaaaa gtgaaaaggg gtgaaattat
     3241 cggctgggta ggcagtaccg gcaaaagcac aggaccgcat tgccactacg aagtacatcg
     3301 caacggagag ccggttgatc cggtttacta tttttataac gacatcaccc ccgagcaata
     3361 cgaccgcctg ctcaaactgg cctccgccag caaccagagc ttcgattaga tttttcagaa
     3421 tagtttaggt tgtttcggaa tagtttttgt tatttttggt gaaggcatgg ataatctcac
     3481 ccagatctca ttgaacttta ttgaagaagc acctcttcgc caggatcttc cggtgaatcc
     3541 cgaagatcag ccactgaata ttggcatccg gatcaagaaa ctcaaacagc cggaaaggat
     3601 ggagcctgtt ccggaacccg cgatcccgga tactaaaccg gcagagccct ttctgaaacc
     3661 taaatcgggc aggggcagaa agtccatccg ccagatttca gaagaagcgg acctgatcca
     3721 gattccggag gacgaacagc ttttcagcaa gcagtactat accatgggag aggtttcgga
     3781 aatgttccgc gtcaaccagt ccctgctgcg tttctgggaa gcggagttca gtattctcca
     3841 gcccaaaaaa aataaaaaag gcgaccggta cttccgcccc gtggatatca agaacctcca
     3901 cctgatctat catctgctcc gccagcgcaa atacaccatc gaaggagcta aggattttct
     3961 cagaaaaaat aaaaaagctg aagagaaatt cgagtccatt aaaaaactgg aacaggtaaa
     4021 ggaatttttg ctggagatga aggcgatgat ccggtaaatg cggttttgcc tacccctttt
     4081 tcggtttatc cgttttcccc tcattacccc ccatcagctt cacagaaaca ctggatcccg
     4141 aaagcgttat ccgcagcatt tccagcagcc ccaggatttc cagatttact ttttgtttga
     4201 tctcgttgaa atccgcgaat gaaataggcg gggtaaaata gtcgatattc agctggaatg
     4261 ccgttccgga aatatcgttc aggaaaaccg ttggattgtc tatataggtt ttactgagta
     4321 tttttttgca ttccccgatc agttgctgaa tggtatcgga cgtggttgac ggatccagct
     4381 gtagccggat ctcccctttt cgctgcgtcc ggagcgactg gttgtccacg atgctgtcca
     4441 ccatttgttt attgggaacg gtaacatagg ttttctgatc cgtgcggatc cgggtgctcc
     4501 gcaggccgat cttttccacc gttccggaaa tattgttcac tttcaggatg tccccggtct
     4561 gaaacggttt atcgaaaaag ataataaacg aggcaatgag gttctctata ctttccttgg
     4621 tggccagagc aagggcagca cccgcaattc ccaaaccggt ccacagcttg ctcacttcaa
     4681 atccgaaaga aatgctcaaa atcatcagta tgccgataat aaccaggatc acttttaaga
     4741 aatcccggaa gaagaccagc atttgatgat ctttttgtcc cccggtcagg ttcgcctttt
     4801 ttttcagcag cagggccacg aaatcgatta tgcgcagcag cagccagata aacatgagaa
     4861 tgagaacggt cttggcggcg gaatgaataa tgatccggga ggagatctca tatatttcaa
     4921 attccagaac ctttggaaaa tgaagttttt caatggccac aatagcaata aaggccagca
     4981 gaaaggtttc caccggagcc agcagcagtt ttacaaagga ttgtttgtcc agccccgaat
     5041 cgagggtgct cgcaaaccgg tagagttgcc cggcgataag gcgggacagg atccgcttga
     5101 aaagaagtcc gataacgatg gtcagaacaa ccagcaggta gcttttcacc gggttatcca
     5161 atataggctg attccagaag tcctgcataa attatccatt cttataaata tgaatgccgt
     5221 ctgttttcaa cacatacaca ttttccggtc gtatggtcat tttgatcgcc ggcaaaaaat
     5281 aggcaggaat ttcctgtttt tcctgttctc ccgttttttc cgtgtatttc aaataataat
     5341 ggccttccca gcccagtatg gtatgatcaa ttacagaaaa atgctgccag ttctgaagcg
     5401 gcaggaattt ctgaaatccg ccataatggt caaaaataaa aactcctttg agggaatcat
     5461 ataaatagac cagcccgccc tggtctgtca ggcgggcggg atccggaatc gaatcggtga
     5521 gttgacggat atccgtcgtt tccaggatca ggctgccgtc atctccgatt tttttcagct
     5581 ttccttccag ctcgtcatag acccagacat tattatcgta ggccagtccg atacttttta
     5641 cctgaaacag gttaaatttc cgaaggtcaa tgctgttgat attattcagg aaacgatcca
     5701 gcattacgat ggtgccgaac tcctgataat ataaaagtat tttgagcgga ttggcaacat
     5761 ccatatagga aatccgccca taccggcgaa cgtcgttaaa tacagccagg gaatccccgg
     5821 cggcattcat ttttttcagc cggttgtctg tactcagcgc atagatattt cccaggttat
     5881 ccacttcaaa atcgatgaag ctgcccggga tgaaatgggc cttcagcccc ggactgtcca
     5941 cttgcgcatc tgcggaaagc agcgcccagc taaggattat atacgaaatc agtttcttca
     6001 cttgcggatg atctggttat taaggctggc atcgaaggat tgaaggctta aggatccacc
     6061 atcaaattcc gcgtaggtaa aataccggat ccagtcgccc aggttgatgt accggctccc
     6121 gttgggaaag gtaaaatcga taggcagatg ccggtgccca aagatcaggt aatcaaaaaa
     6181 ttctttatcc agcacatttt tacaatatcc gatcagccat tcccccccgg ttcccaaaaa
     6241 ccggtcgtct gccaaccccg tagccacccg gcttttacga ctgaaatagt tagccaatcc
     6301 aactcctgcg gcggggggaa gaatgccgaa cagccattga caatatttat tccggaaaat
     6361 ccttttgagg aatttgtatc ctttatcccc gggccccagt ccgtccccat gcccgattaa
     6421 aaacttcttt ccgttccgga taaaagtctt cggctcataa tacaccggga tattcagttc
     6481 cttttcaaaa taattattca tccacatatc gtggttgccg acaaaaaact ggatggggat
     6541 accggaatcg gttaattcgg ccagtttccc caggatacga acatagcctt tgggcacgac
     6601 cgtacggtat tcgtaccaaa aatcgaagag gtcgcctaga ataaagatct ctgcggcccg
     6661 ggaccggatg gattccagaa attgaatgat gtatttttct ctttgcaggc tggtgagccg
     6721 atcgggagat cccagatgga agtcagataa aaaataaatt tttttccctt ctgcgatttg
     6781 catgggcaaa tataaattac tctgcatcga tcaggaaaac atatacgtct ttcggcccgt
     6841 gaacacccac gaccagcgtt ttttcaatat ccgcggtccg gctggggcct gaggccaggg
     6901 tgatcaggga aggcagccct tttttgtatt tatcctgcag gaattccagc ccttcccgga
     6961 tatcgtaaac caactggttt gaatacgcga tacagatatg aaccggggca tatacactaa
     7021 ccgttcttcc gctttcctga gcggcgctta acaggatact gccggtccgc gcaatcagca
     7081 actcgcagga cgtgatggag gcgtctgctt ccggaaggcc gccggcggtt aacaggcctt
     7141 tgagcggctc atcaaggatc cggcgcaggg cggtttccct gcaaaaaatc tgggcccagg
     7201 cgttttttgc gatcagcagc ttcagctgcc tgagcatttc atcctgatct gcacaataaa
     7261 taaatttccc ctgaacctgg gcgagttgtt cggcaaactc tatttctttc tcctggctgg
     7321 agggccggta tacagaacta ttcccctcac tctggggaaa agggatcggt gtcggctccg
     7381 tcagggcctg cctgatcctt tttaaaatat tttcctttgc gggcgatact ttcataaggc
     7441 actcagatcg gttcttcagc aatggggggg acagctaccg gcggcgcggg caaaccgtta
     7501 tccggaagtt tcaattcggg aacatcgtcc agctcatgtt cggaaacatc cagcgccctt
     7561 ttttcttcaa aaggcctttt tccgatgagg gtctccacat cgctctgaaa aagaacttct
     7621 ttttccaata aggcttcggc gagtaaatgg agctgggaat tctttttcgt gaggagctcc
     7681 ct
//
LOCUS       DASYCU010000119         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1770312, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000119 DASYCU010000000
VERSION     DASYCU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770312"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VGM24_02400"
     CDS             <1..1551
                     /locus_tag="VGM24_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154238.1"
                     /translation="CQAFLLRASSLPRSTIFRSDYSDRKVLSGLKEGTFCIPYSFFFN
                     LKKQTDMKGSYPASRRHFLQQMSGTALALAAANSSLAFADQWQNRVDSYSKKISPNDK
                     IRIGVIGIGIQGNNDLEAALKVPGIEMAAACDLYKGRLERAREVYGKDLFVTQDYREL
                     LQRKDIDAVIIATSDHWHSRISIDALNSGKAVYCEKPMVHQISQGLGVIEAWEKSKKT
                     MQIGSQRVSSLATIKAHEAFRSGAIGQLSCIEANFDRQSALGAWEYTMPTDQSTQTVD
                     WKRYIQGMPDQPYDPKKFFWWRNYRAFGTGVAGDLFVHLLSGIHAITDSKGPEKIYSS
                     AQLCYWKDGRDVPDVMAGVIQYPETKEHPEFQLMLRVNFISGNNESGGTTFIGSEGVL
                     TMEGNGYTIHHHKMSKAPGIGGWDALTTYPKAMQEELLKQYNQKYSAEDQRDHSDPDT
                     VFRTPHGYDEHMEHFANFFAGVRNGGPVLEDPVFGFRAAAPCLAANESYFQDKIIRWD
                     PVNMKLIS"
     gene            1563..2645
                     /locus_tag="VGM24_02405"
     CDS             1563..2645
                     /locus_tag="VGM24_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEY4154239.1"
                     /translation="MEKIFSYQDLWRFTKDIFCSIGCPEQDAELAATGLLRADLRGID
                     SHGIARLTGYVRLWEAKRANSRPQIQIVHETPSTGVVDGDSGLGLVVAPFAMDLAIRK
                     AKNAGSGWIAVRNSNHFGIAAQHALKALEEDMIGIVMTNASALVAPTFSIERLLGTNP
                     ICVAVPSGKEPPFVADLATTTAANGKLEVLQRKNESAPEGWIQDKNGNISTSPHELKA
                     GGALLPLGGDREHGSHKGYALGAIVDIFSGVLSGANYGPWVPPFPAYVTIPENMPGKG
                     IGHFFGALRIDGFRKPEEFKKDMDQWLVRFRTAKPGMGHEKVIVPGDPEREMEAIRKK
                     QGIPLMKSVLEDLDWLSSRFRITPLP"
     gene            2989..>3577
                     /locus_tag="VGM24_02410"
     CDS             2989..>3577
                     /locus_tag="VGM24_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154240.1"
                     /translation="MKQIYEPTVKTYSTHIKKTLREAIFPGASAGRKGLAVLAIVLGV
                     LTLQFSSVPVFAQDCATSGSHNQTKNENTYYPGTQATVPAGSTSITLGAVPTGYGGNK
                     ITSGDIILIIQMQGAQIYVPAGSTSTLYGASTSGLESGFITTNLMAGNMEFAVAANNV
                     PLTGGTLNITAGLTYSYAYKAFGTTGQYTYQVIRVP"
BASE COUNT          947 a          861 c          950 g          819 t
ORIGIN      
        1 tgtcaggcat tccttctccg ggcgagctct ctgccccggt cgacgatctt ccgttctgat
       61 tattccgata gaaaagtgct ctccgggttg aaggagggca ctttttgtat accttattcc
      121 ttttttttta atttaaaaaa acagacagac atgaaaggat cttacccggc ttcccgcaga
      181 cattttctac aacagatgag cggaaccgcg ctggcattgg ccgcggccaa ttcttccctg
      241 gcctttgctg accaatggca gaaccgggtg gacagctatt ccaaaaaaat ctccccaaat
      301 gataaaatcc ggatcggcgt gatcggcatt ggcattcagg gcaacaatga tctggaagcc
      361 gcgctgaaag taccgggtat cgaaatggcg gccgcctgcg atctctacaa aggcaggctg
      421 gagcgggcca gggaagtgta cggaaaagac ctttttgtca cgcaggatta ccgggaactt
      481 ctccagcgga aagacatcga tgcagtgatt attgcgacga gtgatcactg gcattcccgg
      541 atatcgattg atgccctgaa tagtgggaaa gccgtttatt gcgaaaaacc catggtgcac
      601 cagatcagtc agggactggg ggtgatcgaa gcctgggaaa aaagtaaaaa gacaatgcag
      661 atcggaagcc agcgggtgag cagcctggca acgatcaagg cacatgaagc atttcgttcg
      721 ggagcgatcg gccagttgtc ctgtatagaa gcaaattttg accggcaaag tgcattgggc
      781 gcctgggaat ataccatgcc gacagaccag tcaacgcaaa cggtggattg gaaaagatat
      841 atccagggaa tgccggatca accctacgat ccgaagaagt tcttctggtg gagaaattac
      901 cgggctttcg gaaccggcgt ggcgggggac ctgtttgtcc acctgctctc cggcattcat
      961 gcgatcaccg attcaaaagg accggaaaaa atctattcat ccgctcagtt atgctactgg
     1021 aaagacggcc gggacgttcc ggacgtgatg gccggagtga ttcaataccc ggaaaccaaa
     1081 gaacatccgg agtttcaact catgctgcgc gtgaatttca tcagcggaaa taacgaatcg
     1141 ggcggaacaa cgtttatcgg atcggaaggg gtattgacca tggaagggaa cgggtatacg
     1201 atccatcatc ataaaatgtc caaggcgccg ggtatcgggg gatgggatgc actgaccacc
     1261 tatcccaagg ccatgcagga ggagttgctg aaacaatata atcagaaata ttccgcggaa
     1321 gaccagcgtg atcattccga tcccgatacg gtattccgca cgccgcacgg ttatgatgaa
     1381 catatggaac atttcgcgaa ttttttcgca ggggtgcgga acggggggcc ggtgctcgag
     1441 gatcccgtgt tcggcttccg cgctgcagcg ccctgcctcg ctgccaacga aagttatttt
     1501 caagataaaa tcatccgttg ggatccggta aatatgaagc tgatatcctg accgctccca
     1561 atatggaaaa aatttttagt taccaggacc tttggagatt tacaaaagat attttctgca
     1621 gtataggttg tcctgaacag gatgcggaac tggctgccac cggtttactt cgggcagacc
     1681 tgcgcggaat agattcgcac ggcatcgccc ggttgaccgg atatgtcaga ctttgggaag
     1741 cgaaaagggc gaacagcaga ccgcaaattc aaattgttca cgaaacaccg tctaccggcg
     1801 ttgtggatgg cgacagcgga ctgggattgg tggttgcgcc atttgccatg gacctggcga
     1861 tccggaaggc aaaaaatgcc ggatcgggat ggatagccgt ccggaattca aatcattttg
     1921 gcattgctgc ccagcacgcg ttaaaggccc tggaagaaga catgatcggg attgtcatga
     1981 ccaatgccag cgccctggtg gcccccacct tttcaatcga acggttgttg ggaaccaacc
     2041 cgatttgtgt ggccgttccc tccggcaaag aaccgccctt tgtagcggac ctggcaacca
     2101 ccacagcagc aaacggcaag ctggaagtgc tgcagcgtaa aaacgaatcc gctccggaag
     2161 gatggatcca ggataaaaac ggaaatattt ccaccagccc ccatgagctg aaagcaggag
     2221 gcgccctgct tcccctggga ggtgaccggg agcatggaag ccacaaagga tatgccctgg
     2281 gggccatcgt ggatatcttt tccggagtgc tcagcggagc caattacgga ccctgggtac
     2341 ctccgttccc ggcctatgtg accataccgg aaaatatgcc gggcaagggc attgggcatt
     2401 ttttcggggc actgcgcatc gacggattcc ggaagccgga agaattcaaa aaagatatgg
     2461 accagtggct ggtccggttc cgtacggcaa aacccgggat gggtcatgaa aaagtgatcg
     2521 tgcccggcga tccggaaagg gaaatggaag ctatccggaa gaaacaggga attccactga
     2581 tgaagtcggt gctggaggac ctggactggc tgtcctcgcg gttcaggatt accccccttc
     2641 cctgacccct tctatatttt cagattaggc aataattcgg ctttcatcta agttattaca
     2701 cttagctata gggaatttta tttcgctgat tattcgttta tcgaatttca tccgatgctc
     2761 atcgaatatt attaggatct gtacgttcag aaggttattt ttcaagaccc tctgtagtgt
     2821 gtaaagaccg atccgtcagc ggcacaactt cagactaaaa tcaaaatcgg aaaaagaatc
     2881 tcaaaatggg aaaattccat ttagagagtt gagtgtaaac ccttatagtt caggaagatc
     2941 tggtttggta tagcaattgg aacagctatg atgtcaaaac cgaaccttat gaaacaaatt
     3001 tacgaaccta ctgtgaaaac atacagtacc catataaaaa aaaccctccg ggaggctatc
     3061 ttcccgggag cttctgccgg cagaaagggc ctggctgttc tggccatcgt tctgggtgtt
     3121 ctgacgctgc aattttcttc ggtccctgta tttgcacagg attgcgcgac ttccggatct
     3181 cataatcaaa caaaaaatga aaatacttat tatcccggca cgcaggcaac tgttcctgcc
     3241 ggttcaacat ccatcacgtt aggtgctgtt ccaaccggat atggcggaaa taaaattact
     3301 tcgggtgata ttatcctgat cattcaaatg cagggcgccc agatctacgt gcctgccggt
     3361 tcaacaagca cgctttacgg tgcgagcacc agtggcctgg agagcggttt tataaccacc
     3421 aacctgatgg cggggaatat ggaatttgcg gttgcagcga acaatgttcc gctgaccggg
     3481 ggcacactga atataacggc agggcttacc tattcttatg cttataaagc gttcggtaca
     3541 accggtcaat atacttacca ggtgatccgg gtgcctt
//
LOCUS       DASYCU010000120         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2061708, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000120 DASYCU010000000
VERSION     DASYCU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061708"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VGM24_02415"
     CDS             <1..821
                     /locus_tag="VGM24_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEY4154241.1"
                     /translation="PLGEKLSTSMRVKAAYNNSKNVIAKITGSKRPDEYIIYTAHWDH
                     LGIGRPDKNGDSIYNGALDNASGTAALLEIARAFKNSAVKPERSILFLAVTAEEQGLL
                     GSEYYSTHPLYPLNKTVADLNMDVMNADGKTKDIVISGMGQNDLEDYVGAVAKAQGRY
                     LAPESHPEAGHYFRSDHFSFAKMGVPALSVGSGIDNAEKGKAYGRKLEEDYTMNHYHQ
                     PSDEYDSSWTFEGGLQDMDLLFRIGKQLASESTWPEWKPGSEFKMIREKSEKAP"
     gene            881..1336
                     /locus_tag="VGM24_02420"
     CDS             881..1336
                     /locus_tag="VGM24_02420"
                     /inference="COORDINATES: protein motif:HMM:NF028184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HEY4154242.1"
                     /translation="MEKDVAMTGKLGFDIQVSHLSANEISFCRNAVENYKRLQNTINF
                     GLLYRLISPYHNNQAALMYADSNSRRAVLFAYNLNTTNEDNFPRLILQGLDPTKKYQV
                     EEINGQEGKRTAFKQSGQEFTGDYLMKEGLNWWLRAPLTSSILELNAVE"
     gene            complement(1350..>1989)
                     /locus_tag="VGM24_02425"
     CDS             complement(1350..>1989)
                     /locus_tag="VGM24_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY4154243.1"
                     /translation="TKLVNDLLQIVLKYDFDGVDVDLEGSDIDENYENLAVDLATVFK
                     SKRKLVTSAIAVFYKDQLSDRALAQYDFVNIMSYDHTGPWDPSKPGPHSTYEQAVEDL
                     DYFSTVRKIPKEKMVLGVGFYGYGFGPARSSPPTEMNFGEIVSAFPGSENADQWNMPG
                     GAVLYYNGIPTIRRKTMLAKEKASGIMIWQIMGDAPGEKSLLKVIYDTAYAK"
BASE COUNT          567 a          464 c          482 g          476 t
ORIGIN      
        1 ttccgctcgg agagaaacta tccacttcca tgcgggtgaa agcggcctac aataactcca
       61 aaaacgtgat tgccaaaatt accggaagca aacgaccgga tgaatatatt atttacaccg
      121 cacattggga tcatttaggt atcggccgtc cggataaaaa cggcgattcc atttacaacg
      181 gagccctgga taatgccagc ggtaccgccg ccctgctgga aatcgccaga gcttttaaga
      241 acagtgccgt aaaaccggaa cgcagcatcc tttttctggc cgttacggca gaagagcagg
      301 gcttgctcgg atccgaatat tacagcactc atcctctgta tcccttaaat aaaaccgtgg
      361 ccgatttgaa tatggatgtt atgaatgccg acggaaaaac aaaggacatc gttatttcag
      421 gaatgggtca gaatgatttg gaggattacg ttggcgcagt ggctaaagcg cagggaaggt
      481 acctggctcc cgaatctcat ccggaggcgg gacattattt tcgttccgat catttcagtt
      541 tcgcgaaaat gggcgtgccg gcgctgagtg tgggaagcgg aattgataat gcggaaaaag
      601 gaaaagcgta cggcagaaag ctggaagagg attacaccat gaatcattac catcagcctt
      661 cggacgaata cgattccagc tggacatttg agggaggatt gcaggatatg gatctcttgt
      721 tccggatcgg aaaacaactg gcttcggaaa gtacctggcc ggaatggaaa cccggatccg
      781 agtttaagat gatccgggag aaatcggaaa aagctccctg atccggtgtc aaaaggtaac
      841 ggttgaagga catgtatatt tttaataaga aatatcgtag atggaaaaag atgtggccat
      901 gaccggcaaa ctgggttttg acatccaggt gagccacctg agcgcgaacg aaataagttt
      961 ttgccggaac gcggtggaaa attacaaaag acttcaaaac acgatcaatt tcggattatt
     1021 gtaccggctc atttctccct atcataacaa ccaggctgcc ctgatgtatg cagacagcaa
     1081 tagccggcgt gcggtcctct tcgcttataa cctgaacacg accaacgaag acaattttcc
     1141 caggctgatc ctccaggggc tggatccaac aaaaaaatac caggtagagg aaatcaatgg
     1201 ccaggaagga aagagaacag ctttcaaaca aagcggtcag gaatttaccg gggattatct
     1261 gatgaaggaa ggtctcaact ggtggctgcg cgcgccattg accagctcaa tattggaatt
     1321 aaatgccgtc gaataaaaaa gattttccat tatttggcat aagcggtatc ataaatgacc
     1381 ttgagcagcg atttttctcc gggagcgtcg cccatgatct gccagatcat gatcccggat
     1441 gctttctctt ttgccagcat ggttttccgc cggatggtag gaatgccatt atagtacaga
     1501 acggcccctc cgggcatatt ccactgatcg gcgttttcag atccgggaaa cgcactgaca
     1561 atttccccga aattcatttc cgttggcgga gagctgcggg ccggtccgaa gccatatcca
     1621 taaaacccca cacctaaaac cattttctcc tttggaattt tcctgacggt actaaaataa
     1681 tccaggtcct ccaccgcctg ctcataggtt gaatgaggcc cgggtttgga cggatcccag
     1741 ggtcctgtgt ggtcataact cataatattg acaaaatcat attgggccag ggccctgtcg
     1801 ctcagctgat ccttataaaa aacggctatg gctgaagtga ccagtttacg cttcgattta
     1861 aataccgttg ccagatcaac cgcgaggttt tcataatttt catctatatc actcccctct
     1921 aaatcgacat ccacgccgtc aaaatcatat ttgagaacga tttgcagcag gtcattaacc
     1981 agttttgtc
//
LOCUS       DASYCU010000121         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5106266, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000121 DASYCU010000000
VERSION     DASYCU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5106266"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VGM24_02430"
     CDS             complement(<1..797)
                     /locus_tag="VGM24_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4154244.1"
                     /translation="MDFLKQLNIHSENSGTSTGSNWMAGSAKPLDSFSPVDGKRIASV
                     TLTGNASYNEVVEKAQKAFQVWSTWPAPKRGEMVRQIGEALREKKTALGRLVSYEMGK
                     SLQEGLGEVQEMIDICDFAVGLSRQLHGFTMHSERPRHRMYEQWHPMGVVGIISAFNF
                     PVAVWSWNSMLAWVCGNVCIWKGSEKTPLCSVACQQIVSSIFEKNEVPEGTSCLITGD
                     REAGEWMGDDSRISLVSATGSTRMGKALGAQVAGRMGKSLLELGGNNA"
     gene            complement(850..1548)
                     /locus_tag="VGM24_02435"
     CDS             complement(850..1548)
                     /locus_tag="VGM24_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY4154245.1"
                     /translation="MMTCIAIDDEPLALQVLKKYIEKIYFLECKGLFTDPEEARKFLE
                     SGSVDIIFLDIQMPDINGIEFSKSLSMKHTAVIFTTAFSEYAIEGFNVDAVDYMLKPI
                     EYDRFLKGVYKAKEYVDYLNSQELQQGYIFVKSDYQMVKILLKDIIYIEGLDDYIKIY
                     LPQRSVLTLMTLKTITQKLPAKEFLRVHRSYIVPVSKIDHISKSKIRVMDKDIPIGIS
                     YSESFFSAMEQRMQ"
     gene            complement(1548..2594)
                     /locus_tag="VGM24_02440"
     CDS             complement(1548..2594)
                     /locus_tag="VGM24_02440"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY4154246.1"
                     /translation="MNKKAAQIIVHIAAWGCFLMLPLIFYPRPVDASFIPEQAVTPYF
                     FISNFFFIAFYYFNTHVLFPRLLEKNRLLSYYLIIIGIMIFFGSMPRLYQSYFGHFQR
                     FSTAIPLHPYRNFHPAILSPGSIAIFLLVFLFSTGIKVMQKWMNSERRNKQIENEKLH
                     AELSFLKSQINPHFLFNTLNNIYSLAESKSDLTPVAVMKLSSIMRYVLTEARQDTVPL
                     EKEMQFINDYIELQKIRNTDKTSVGFTVVGDISGKKISPLILLPFTENAFKYGVSTRE
                     LSPINILIESHKNHIHFKIKNNKHNNGKNSLVNTGIGINNTRRRLELLYPGKYDLNIE
                     DEPQTYTIHLNIHS"
BASE COUNT          798 a          673 c          735 g          765 t
ORIGIN      
        1 gcgttattgc cgcccagttc gagcagggat tttcccatcc ggccggccac ctgggcgccg
       61 agggctttcc ccatgcgggt ggaaccggtg gctgaaacca ggcttatccg gctatcgtca
      121 cccatccatt cgccggcttc ccggtctccg gtaatcagac agctggtccc ttcggggacc
      181 tcattcttct caaaaatgga ggacacaatc tgctggcagg caaccgaaca taaaggcgtt
      241 ttttcggaac ctttccaaat acagacattc ccgcaaaccc aggcgagcat gctattccag
      301 ctccaaacgg caacgggaaa attgaaggcg gaaataatgc cgaccactcc catcggatgc
      361 cattgttcgt acatccggtg ccggggtctt tcgctgtgca tcgtaaaacc atgcagctgc
      421 cgggaaaggc caacggcaaa atcacagatg tcaatcattt cctgaacttc acccaggcct
      481 tcctgaagac tttttcccat ttcataagaa accagccggc ccagggccgt ctttttttcg
      541 cggagcgctt cgccgatctg gcggaccatt tcgccccgtt tcggagcagg ccaggtgctc
      601 cagacctgga aagccttttg ggctttctcc actacttcat tataggaagc attgccggtg
      661 agggttacgg aggcaatccg ttttccgtcc accggagaaa acgagtccag cggttttgcg
      721 cttcccgcca tccagttgct cccggtggat gttccgctgt tttcggagtg aatattgagc
      781 tgtttgagaa aatccatgga attcgattga acccgcaagt tataaagaac aggaataatg
      841 ccgggtatcc tattgcatgc gctgttccat ggcggaaaag aagctttcac tgtaactgat
      901 gccgatcgga atatccttgt ccattaccct gattttgctc ttgctgatat gatcaatttt
      961 gctcacggga acgatatagg accggtgaac gcggagaaac tccttagccg gcagtttctg
     1021 ggttatggtc ttgagggtca tcagggtaag gaccgatcgc tggggaagat agatcttgat
     1081 atagtcatca agcccctcga tataaataat atccttcagt aagatcttca ccatctggta
     1141 atcggatttt acaaaaatat agccctgctg aagttcctgg ctgttgaggt aatccacgta
     1201 ttccttggct ttatagacgc ccttcagaaa acggtcgtat tctatgggct tgagcatgta
     1261 atccacggcg tccacattga atccctcgat cgcgtattcg ctgaaagccg tggtaaaaat
     1321 gacggccgta tgcttcatgg ataaggattt ggaaaattca attccattga tgtcgggcat
     1381 ttggatatcc aggaaaatga tatccacgct gccggactcc aggaatttac gcgcttcttc
     1441 gggatccgtg aaaagtcctt tacactccag gaaataaatt ttttcaatgt atttttttaa
     1501 tacctggagg gcaagcggtt catcgtctat cgcaatacag gtcatcatca ggaatgaata
     1561 tttaaatgaa tggtataggt ctggggttcg tcttcaatgt tcagatcata tttgcccgga
     1621 tatagcaatt ccagtctccg gcgcgtattg ttaatgccga tcccggtatt gaccagggaa
     1681 ttctttccat tattgtgttt attgtttttg atcttgaagt ggatatggtt tttatgggat
     1741 tcaatcagga tattgatggg cgaaagttcc cgggtgctta ccccgtattt gaatgcgttt
     1801 tccgtgaagg gcagcaggat cagcggggag atcttctttc cggatatatc gcccaccacc
     1861 gtgaagccga ccgatgtttt gtctgtgttc cggatcttct gcaattcaat ataatcgttt
     1921 atgaactgca tttccttttc cagcggcacg gtatcctgcc gggcttccgt aagtacataa
     1981 cgcatgatgc tggataactt cataacggcc acgggagtaa gatcagattt gctttctgcc
     2041 agcgaataaa tattattcag cgtgttaaaa agaaaatgcg ggttgatctg ggatttcaaa
     2101 aaagagagtt cggcgtgaag cttttcattt tcgatctgtt tgttacgcct ctccgaattc
     2161 atccatttct gcatcacctt aataccggta ctgaataaaa agaccagcag gaaaatggca
     2221 atagatccgg gcgagaggat ggcgggatga aaattccggt acggatgcag ggggatggcc
     2281 gtggagaagc gctggaaatg tccgaaatag gactgataca accggggcat gcttccgaaa
     2341 aagatcatga tcccgataat aatcagataa taggagagga gcctgttctt ttccagcagc
     2401 cgggggaaaa gcacatgggt attgaaataa taaaatgcga taaaaaagaa attgctgata
     2461 aagaagtatg gagtaaccgc ctgttccgga atgaaagaag catccaccgg cctgggataa
     2521 aaaatcaggg gaagcataag gaagcaaccc caggcagcaa tatgaacaat aatctgggcg
     2581 gcctttttat tcaaagagaa aaatttaagg ttaaaagaac ccgctttcca cggcttgtat
     2641 gacttcagag taatcaaaac taaagttgtt ttttaaagag ataaagttaa agaccggtca
     2701 gaagcggata tatcgcggat aatagacgaa tgccgtatta taatcggtga atcctccccg
     2761 ggtaaaatta ataataaaga aggaagggga aggcccaacc ccacgctcaa ttcgtaccgg
     2821 gtatgaaggg ccgccggcgc ggcaggaaag gaaacacaga aaaaatagga atattacggc
     2881 agaataagcg ccggttcggt agaatggcaa gcagatcaat agtttacctt gaaccgccgg
     2941 ccatgggccc caggttctgg tgaccgtatt t
//
LOCUS       DASYCU010000122         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4672869, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000122 DASYCU010000000
VERSION     DASYCU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4672869"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VGM24_02445"
     CDS             <1..733
                     /locus_tag="VGM24_02445"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY4154247.1"
                     /translation="SFIWNSKHNIIHHSYTNVAGVDDDIDIQPWMRMSTSQKRLRLHK
                     YQHFYFWFLYSLLYILWIFLLDYNKYFTSKVGEVPLKKMKLTDHINFWAFKLLNGFLF
                     VALPIYMTGFMPWLTGFLIVTTVAGLVISVVFQLAHTVEHTAFPVANIETGKMENEWA
                     IHQVMTTANFATGNRLVSWLVGGLNFQIEHHLFPKISHVHYPALSKIIRQACADHGLV
                     YIEYRRVHQAIASHVHFLKQMGKAS"
     gene            798..>1535
                     /locus_tag="VGM24_02450"
     CDS             798..>1535
                     /locus_tag="VGM24_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEY4154248.1"
                     /translation="MGKIRIGMIQMSCVRDKSANLKKAAGKIREAAAAGAQIICLQEL
                     FTSLYFCDQEDYDNFGLAEPIPGPSTDTLRELAAETNTVIIGSLFEKRAAGIYHNTAA
                     VIDADGTYLGKYRKMHIPDDPAFYEKFYFTPGDLGYQVFTTKYARIGVLICWDQWYPE
                     AARISSLKGAEILFYPTAIGWSTAQDLPVNEEQYNAWQTIQRSHAVANGVHVVSVNRV
                     GLEQEGAMKFWGGSFISNPFGRVLSQAS"
BASE COUNT          385 a          404 c          367 g          379 t
ORIGIN      
        1 cagttttatc tggaacagca aacacaatat cattcatcat tcctatacca atgtggccgg
       61 cgtggatgat gatattgaca tccagccctg gatgcgcatg agcacttcgc aaaaaaggct
      121 gcggctgcat aaataccagc atttctattt ctggttcctg tactccctcc tctacattct
      181 gtggatcttc ctgctggatt acaataagta ttttaccagc aaggtcggag aagtgccgtt
      241 gaagaaaatg aaattaaccg atcatattaa tttctgggcc tttaaactac tgaacggatt
      301 tcttttcgtg gcgctaccca tctacatgac cggctttatg ccctggctca ccggattcct
      361 gatagtgacc acggtggccg gcctggtgat cagcgtcgta ttccagctgg cccatacggt
      421 ggaacatact gcatttccgg tggccaatat agaaaccggt aaaatggaaa acgaatgggc
      481 cattcaccag gtgatgacaa cggccaattt tgccaccggc aaccggttag tctcctggct
      541 tgtcggcgga ttgaattttc agatcgaaca tcatttattc cccaagatct cccatgttca
      601 ttacccggct ctaagtaaga tcattcgcca ggcctgcgca gatcacggcc tcgtatatat
      661 agaataccgc cgcgtacatc aggccattgc ctcccatgtc catttcctga aacaaatggg
      721 caaagcgtcc tgaacccttt ccgtataatc gtcccgatcc gctaattttg cgtttcaaca
      781 ggattaatca cagcaatatg ggaaagatca ggatcggaat gatacagatg tcctgcgtcc
      841 gggacaaatc cgccaatctc aaaaaagccg ccggaaaaat ccgggaagcc gccgcggccg
      901 gcgcacagat catttgcctg caggagcttt ttacttctct ttatttctgt gaccaggagg
      961 attacgacaa tttcgggctg gctgaaccca tacccggacc ttccaccgat acgctccggg
     1021 aattggccgc tgaaacaaat acggtgatca tcggttcgct ttttgaaaaa agagcagccg
     1081 gcatttatca taataccgcc gccgtaattg atgcggacgg aacctatctc ggaaaatacc
     1141 gcaaaatgca tatcccggac gacccggcct tttacgagaa attttatttt actcccggcg
     1201 atctgggtta ccaggtgttc acgacgaagt atgccaggat cggggtgctg atctgctggg
     1261 accaatggta tccggaagca gcccggatca gttctttgaa aggggccgag atcctttttt
     1321 atcctacggc tatcggctgg tcaaccgccc aggatctacc ggtcaacgaa gaacaatata
     1381 atgcctggca gaccatccag cgaagtcatg ccgtggccaa cggtgtccat gtggtcagcg
     1441 tgaaccgggt gggactcgag caggaagggg cgatgaaatt ctggggcggt tcattcatat
     1501 caaacccgtt cgggcgggtc ctttcccagg cttcc
//
LOCUS       DASYCU010000123         7464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2206204, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000123 DASYCU010000000
VERSION     DASYCU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206204"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VGM24_02455"
     CDS             complement(<1..353)
                     /locus_tag="VGM24_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4154249.1"
                     /translation="MEIKRCGSQPSAKGSTEYFTGTARIDPLFTPAEPARVSMAMVTF
                     EPGARTAWHTHPFGQTLIVTSGCGWANREGGPREEIRAGDVVYFSPGEKHWHGATATT
                     AMSHIAVQEKLNGSPV"
     gene            complement(492..1712)
                     /locus_tag="VGM24_02460"
     CDS             complement(492..1712)
                     /locus_tag="VGM24_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY4154250.1"
                     /translation="MFKTEYRKIISVLCKHFGFDRIAVAEDIASDTFLTAAETWGQKS
                     IPPNPVAWLYNVARNKAKNQLLREANFANKISKELGAPADNNTLEIDLSPENINDSQL
                     QMMFAICNPAIPTDAQIGLSLRILCGFGIAEIAEAFLTSTETINKRLFRAKEKLRDEK
                     IQIEIPGPSEMDNRLGAVLTTIYLLFSEGYYSSTRDQLIRKELCLEAMRLCLMLVENR
                     ITNKPEANALLSLMCFHSSRFDARTNREGQLVIYEEQDTSLWDTELIRKGEYFLNCAA
                     TGNKLSRYHIEAAIAYWHTHKADTGEKWENILQLYNQLLQIQYSPVAALNRTFALSKA
                     NGKPEAIAEAEKLNLTGNHLYYSLLGNLYTGIDNEKAIKYLKKAIALAKTQVEKNSIR
                     VVMEKINGHTFPAG"
     gene            complement(1766..2107)
                     /locus_tag="VGM24_02465"
     CDS             complement(1766..2107)
                     /locus_tag="VGM24_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY4154251.1"
                     /translation="MKEFLLLFRRDASQMGELSPGEMQAAIKPWQDWMGSLAAQDKLA
                     GSGNRLDSDGRVLRGNTVTNGPYVEVKESIGGYIMVRAKDIDEAAGLAKGCPILKSGG
                     NVEVRPVVSTD"
     gene            complement(2129..2587)
                     /locus_tag="VGM24_02470"
     CDS             complement(2129..2587)
                     /locus_tag="VGM24_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY4154252.1"
                     /translation="MEQRVNFYENERAIGALAALGRYLAKSPVEKGLLHLIHFRVSQI
                     NGCAFCLDMHAKDLRAEGESEQRLYVLDAWREAPFYTERERAALAWAESLTIIKGGLV
                     PDEIYAAARKQFSEAELTDLTIAVIQINSYNRINIAFGSAVGTYEVGQFN"
     gene            2763..4154
                     /locus_tag="VGM24_02475"
     CDS             2763..4154
                     /locus_tag="VGM24_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY4154253.1"
                     /translation="MDGTKGISLTGRIRIRLKRIFDRVGNEKLKNNFLQAVPFWIASL
                     ATGLVAVFYSKVFLLAESTTSHFFQKTPWLLFLVTPVCFLTAWWFIVRLDPFARGSGI
                     PQVMAAIELANPKDNHKIKKLLSLRIIGVKMLSSFAMILGGGLIGREGPTIHIAGSIF
                     RKINQWLPSWWPKISKRNMIMTGAAAGLAAAFNTPLGGIVFAVEELTKTHISYFKTAL
                     FSAVIISGLTAQALFGPYLYLGYPGVNNLSVYIFLGVILVALLSGLAGSITSRLMLSL
                     MKWKAGFKKRNHHIIYVLICGFAVALLAFFSNPGLIGSGKQIMTTVLFTPEKYLHWYA
                     PLVRITGSIFSYTTGAAGGVFAPALTSGASIGSVLSSWMHLSDSDTNLLILSGMVGFL
                     TGVTRTPFTSAILVLEMTDRHSLIFYLMLAGMLAGIVSVLVDKHSFYDHLRVQFLREM
                     ELEEMTKRNQKND"
     gene            complement(4179..4631)
                     /locus_tag="VGM24_02480"
     CDS             complement(4179..4631)
                     /locus_tag="VGM24_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4154254.1"
                     /translation="MNKKGYEWAPLFLRLIIGYGFMAHGLAKLSRGTAGFEKLLAQTG
                     VPFPHSASRIVPYVELLGGFAILTGSFVSLVAIPLVVTMLVAMFTVQIKYGFSSVNTI
                     GLTSEGPLFGPPGYEINLLYIAGLISLILSGGGVFSVDALIGRKKRKK"
     gene            complement(4670..5965)
                     /locus_tag="VGM24_02485"
     CDS             complement(4670..5965)
                     /locus_tag="VGM24_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HEY4154255.1"
                     /translation="MKQNQRKPWLMLSAILFLASCDKHKDLPTRSGVITIENVLDSKP
                     LVESGTFKGAGSPALILPGQSVSFTFSAAKNQALSFATMYGWSNDLFFAPGNPGIKLY
                     SDDGTPVTGDVSAEIKLWDNGTRINQAPGMNVTHPGTAEAADKTIKEVNGADDYGNSY
                     LAASRLMKVSLAYNGNSSFTVTLLNNSGGTANETPFSPGVWGISYVVGGSLLLPAPIY
                     SANALSANGLTDIAEMGDNTAAHAYLTRITGIFTPLSPVLVVVYHGVGNPIYQVGEKD
                     RGEGLKDLAQKGNADILAATLKGKPGIRSVYVLKVPGTTVLLPKINGASGGKVSQDLE
                     IAGGDKIAIATMYGFSNDWFFATTADDMGSFGKGSWSPSIGLFDDGTAINQFPGAGVT
                     QFNLAGTPLEESKPIEAVPNPNNFTTLPDIDRIIKVTFQ"
     gene            6099..6803
                     /locus_tag="VGM24_02490"
     CDS             6099..6803
                     /locus_tag="VGM24_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4154256.1"
                     /translation="MPKVLIIEDDIHIVELLSIHLNDLGCEVVPVSNGQDGLELARDR
                     CFDLIILDIMLPALNGMEICRRIRQTDRHTPILILSARSEEIDKVVGLETGADDYLTK
                     PFSVREFIARVKVIFRRNEEHSPAGEKGALSPVIRFSGLEIDTEKRKVTLDRNRVELS
                     PKEFDLITLLASNPGKSYNRKRLLNLVWGYDFEGYEHTVNSHINRLRGKIEKDISNPK
                     FILTTWGVGYRFNEEL"
     gene            6813..>7464
                     /locus_tag="VGM24_02495"
     CDS             6813..>7464
                     /locus_tag="VGM24_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY4154257.1"
                     /translation="MSLNTSRGNLLFWKIAAVFSGLLILLGFIFVLIASRLSRSYYTM
                     AHQELYGNVASHLATFTHPLKNGKPDTAVTHDIIHSIMVANPSVEVYLLDTNGKITDY
                     VVPDKSVRNHRVNISKVKEWLAVKGGERPLGDNPKQPDEPGIFSAAPVYENGQLTGYV
                     YAVLASEKQREILAALNSQLYHRLGIYVFFAALFIAFIVGLITFFLITNSISQVSGV"
BASE COUNT         1802 a         1899 c         1776 g         1987 t
ORIGIN      
        1 accggcgaac cattcagttt ttcctgtacg gcaatatggc tcatggccgt ggtcgcggtg
       61 gcgccatgcc aatgcttttc gcccggcgag aagtaaacca catctccggc ccggatttct
      121 tctcttggcc ctccttcccg atttgcccag ccgcagccgc tggtaacaat caaggtctga
      181 ccaaaaggat gggtatgcca tgctgtgcgg gcacccggtt cgaacgtaac cattgccatc
      241 gaaacccggg cgggttctgc tggcgtaaag aggggatcga tgcgtgccgt tccggtaaaa
      301 tattctgtag agcctttagc cgaaggctgt gacccgcatc gtttaatttc catattttcc
      361 tttttaaata ctgattaagt tctttttccc cttgaaaaaa cgggctttct gtttttgcca
      421 accgctttat cctctgttgc aaaggtctca cttatttttc agtaagtagg aaatactatc
      481 cacggaacac ttcacccggc ggggaatgta tggccattga ttttctccat aacaacccgg
      541 atggaatttt tttccacctg ggtctttgcc agggcaatcg ctttcttcag atactttatg
      601 gccttttcat tatcaatccc cgtatacagg ttacccagca gggaatagta aagatgatta
      661 ccggttaaat tcagtttttc ggcctctgct atggcctccg gtttcccgtt tgccttggac
      721 agggcaaagg tccggttaag cgcagcaaca ggtgaatatt gaatctgcag caactggtta
      781 tataattgca gaatattttc ccatttttct cccgtatccg ccttgtgcgt atgccagtac
      841 gctatggccg cttctatatg gtacctggac agcttattac cggttgccgc acagttgagg
      901 aaatattccc ctttgcggat cagttcggta tcccacaagc tggtatcctg ctcctcatag
      961 ataacaagtt gtccctccct attggtcctt gcatcaaacc gggacgagtg aaaacacatc
     1021 agggaaagca gggcgtttgc ttccggctta ttggtgatcc tgttttcaac cagcatcagg
     1081 cacaagcgca tggcctccag gcaaagttcc ttccggatga gttggtcccg ggtactggaa
     1141 taatagcctt cgctgaataa taaataaata gtcgtcagaa cagctcccag ccggttatcc
     1201 atttccgaag ggcccggaat ttcgatctgg attttctcgt cccttagttt ctccttggcc
     1261 cggaacaacc gtttattgat cgtctctgta ctggttaaaa aagcttcggc tatttcagcg
     1321 atcccaaatc cgcataaaat gcggagcgaa aggccgatct gcgcgtccgt aggtatagcc
     1381 ggattgcaaa tggcaaacat catctgcagc tggctgtcat taatattttc aggcgacaga
     1441 tcgatttcca gggtattgtt gtccgctggg gctcccagtt cctttgatat cttattggca
     1501 aaattcgctt ctctcaggag ttgatttttc gccttgttcc tggccacatt gtacagccag
     1561 gctaccgggt tggggggaat gcttttttgc ccccaggtct ccgcagcggt cagaaaagta
     1621 tcgctggcaa tatcttctgc aacggcgatc cggtcaaatc caaaatgttt acagagtaca
     1681 gaaataatct ttctgtactc tgtcttaaac aaatgcgcta ttaattcagg gtgttgcata
     1741 tgccagcaaa gctacccagg ttgtatcaat cggtcgatac caccggtctt acctccacat
     1801 tgccgcctga tttcagaata ggacagcctt tcgccagccc ggctgcctcg tcaatatcct
     1861 ttgccctgac catgatgtat ccgccgatgg attctttcac ctcgacataa gggccattgg
     1921 taacggtgtt tccgcgcagc acccggccat cagaatcaag gcggttacct gaaccggcca
     1981 gcttatcctg tgcggcaagg ctgcccatcc aatcctgcca tggctttatc gccgcctgca
     2041 tttccccggg ggataattcc cccatttgcg atgcatcccg acggaataat aaaaggaatt
     2101 ctttcattgt atttttgttt aaagatgatt aattgaattg tcccacttcg tacgtgccca
     2161 cggccgaacc aaaggcaata ttgatccggt tatagctgtt aatctggatc acggctatcg
     2221 tcaggtcggt cagctctgct tctgaaaatt gttttcttgc tgcggcataa atttcatccg
     2281 gcacgaggcc tcctttaatt atggtaagcg attcggccca ggcaagcgct gcccgttcgc
     2341 gctctgtata aaacggcgct tcccgccagg catccagtac atacagccgc tgttccgatt
     2401 ccccttccgc ccgcaggtct ttggcgtgca tgtccaggca aaaagcgcag ccattgatct
     2461 gcgatacccg gaaatgaatt aaatgcagca ggcctttttc taccggtgat tttgccagat
     2521 acctgcccag ggcagccaat gccccaattg ctctttcgtt ctcgtaaaaa ttcactcttt
     2581 gttccaattt tcttaaattt ttattgttat gaaatgtttg aattacataa ccataacaat
     2641 cgaaccttct aaagttggac agaatttcag aaaaaaaccg gaattacgga aaagtctgcg
     2701 aaaacgattg attattccct ctggtccgtt tgcggttatg ggcagtgttt tatctttgcc
     2761 ggatggacgg aacgaaagga ataagcctga ccggaagaat aagaataagg ctaaaacgga
     2821 tttttgacag ggtcgggaat gaaaaactca aaaataattt tctgcaggcg gtcccttttt
     2881 ggattgcgtc gctggctacc ggactggtgg cggtttttta ctctaaagtg tttctactgg
     2941 cggaaagcac cacctctcat ttctttcaga aaaccccctg gttattattt ttagtaaccc
     3001 cggtgtgttt tttgaccgcc tggtggttca ttgtccgtct tgatcccttt gcccggggaa
     3061 gcggaatccc gcaggtgatg gccgccatcg aactggccaa tccgaaagac aaccataaga
     3121 taaaaaaact gcttagtctg cggatcatcg gggtaaaaat gctttccagt tttgccatga
     3181 ttctcggggg cggactgatc ggcagagaag ggcccaccat tcatattgcc ggttctattt
     3241 tccggaaaat aaaccaatgg ctgccgtcct ggtggccgaa gatatccaaa cggaatatga
     3301 tcatgaccgg agccgcggcc ggcctcgccg cggccttcaa taccccgctt ggcggaatcg
     3361 tatttgccgt ggaggaactc accaaaacgc atatcagcta tttcaagacg gccctttttt
     3421 cggcagtcat tatatccggg cttactgccc aggctttatt cggtccctat ctgtatctgg
     3481 gatatccggg ggtaaataat ctttcggttt atatcttttt gggagtgatc ctggtagcgc
     3541 tgctctccgg actggcagga agtataactt cacgcctcat gctttcgctg atgaaatgga
     3601 aagccggatt taaaaagagg aatcaccata ttatttatgt tttgatatgc ggattcgcag
     3661 ttgccctgct ggcctttttt tcaaaccccg gcttgatcgg ttcaggaaaa cagatcatga
     3721 ccaccgtcct tttcacacca gaaaaatacc tgcactggta tgcccctctg gtccgtatta
     3781 cgggttcaat attttcctat actaccggtg cggccggcgg tgtatttgcc ccggctctga
     3841 cctccggcgc cagtattggg tctgtattat cttcctggat gcacttgtcg gattccgata
     3901 ccaacctgct cattctttcc ggaatggtcg gatttctgac cggcgttacc cgaactcctt
     3961 ttacgtctgc gatcctcgtt ctggaaatga ccgaccggca cagcctgatc ttttatttaa
     4021 tgctcgccgg tatgctggca ggaatcgtct cggttctcgt tgacaaacat tccttttacg
     4081 accatctaag agtacaattc ctcagggaaa tggaactgga agaaatgaca aagagaaacc
     4141 agaaaaatga ttaaccgtca gtaagcggac cgcaaagctc atttcttcct ctttttccgg
     4201 ccaatcaggg cgtctacgga aaaaacacct cctccgctga ggataagaga tatcaaacct
     4261 gcgatgtata gcagatttat ctcatacccg ggggggccga acagcggtcc ttccgaagtt
     4321 aatccaatgg tattaacaga actgaatcca tatttgatct gaacggtaaa catggctacc
     4381 agcatcgtaa ccacgagggg aatcgcaacc aggcttacaa aactgcccgt taaaattgca
     4441 aatccgccca acagttcaac atagggcact atccgggaag ctgaatgggg aaagggaacg
     4501 ccggtttggg caagcagttt ttcaaaaccg gccgttcccc tgctcagttt ggcaaggcca
     4561 tgagccataa agccatagcc aatgatcagg cgcaaaaata aaggcgccca ctcatatcct
     4621 tttttattca tctgtcatca ttttcgcaat ccggagacga tcattgaacc tattgaaaag
     4681 tcactttgat gatacgatca atatccggca aagttgtaaa attattggga tttggcaccg
     4741 cctcaatggg cttgctttcc tccagcggag tgccggccag attgaactgc gtaacgccgg
     4801 caccgggaaa ctgattgatc gccgttccgt catcaaacaa tccgatggag ggagaccagc
     4861 tcccctttcc gaaactgccc atatcgtcgg ccgttgttgc aaaaaaccag tcgttggaaa
     4921 aaccatacat cgtggcaatg gcaattttgt cgccccctgc tatttcaaga tcctgcgaga
     4981 ccttgccgcc actggcgccg ttaattttag gaagcagaac cgttgtgccg ggaactttca
     5041 gcacatatac gctccggatg cctggcttcc ctttcaatgt tgctgccaga atatccgcat
     5101 tccccttttg cgccagatcc ttcagtcctt ccccgcgatc cttttcacca acctggtaaa
     5161 tgggattccc gactccgtgg taaaccacca ccaataccgg cgacaaaggt gtaaaaattc
     5221 ccgtaatgcg ggtaagatag gcatgcgccg cagtgttatc gcccatttcc gcaatatccg
     5281 tcagcccgtt agcgcttagg gcattggcgg aataaatggg agccggcagc aggagactgc
     5341 cgccaaccac ataggaaata ccccagacgc ccgggctgaa tggcgtttca ttcgccgtgc
     5401 cacctgaatt attcaggagg gtgaccgtaa agctggagtt gccattgtag gccagagaca
     5461 ctttcatcaa tcgggaggca gccagatagg aatttccata atcatccgca ccatttacct
     5521 ccttaatggt tttatctgcc gcttccgctg ttcccggatg ggttacattc atacccggag
     5581 cctggttgat gcgggtgcca ttatcccata gttttatttc cgcggaaacg tcccccgtga
     5641 ccggcgtgcc atcatcgctg tataatttaa tgcccggatt tccgggggca aaaaacaggt
     5701 cgttgctcca gccatacatg gtggcaaagc tcagggcctg atttttggcg gcggagaaag
     5761 taaatgacac cgactgcccg ggaagtatca gcgccggcga tccggcgccc ttgaaggtcc
     5821 cggattccac cagcggttta ctgtccagga cattttcaat ggttatcact ccggatctgg
     5881 ttggcagatc cttatgtttg tcgcatgatg ccaggaataa aatcgcagac agcatcagcc
     5941 atggttttcg ctggttttgt ttcattttgt ttgttttttg atacagcaaa ataagcgtga
     6001 cgatatccca aagaggttgc ggaattataa caaatgtatc accgcgctat tcggatacta
     6061 tttttacgga cttttgattt cccattcata tcaacccaat gccgaaggtc ctgatcattg
     6121 aagacgatat ccatatcgtg gaactgctga gcattcatct gaatgatctt ggttgcgaag
     6181 tggttccggt ttccaacggc caggacgggc tggaactggc cagggaccgc tgcttcgatc
     6241 tcatcatcct ggatattatg ctgccggcac tgaatggcat ggagatttgc cgccggatca
     6301 ggcaaacgga ccggcatact cccattctga ttttgtcggc caggtcggaa gaaattgata
     6361 aagtggttgg ccttgagacc ggcgcggacg attacctgac aaaacccttt agcgtacggg
     6421 agttcatagc ccgggtgaag gtcatattcc ggagaaatga ggaacattcg ccggcaggcg
     6481 aaaaaggggc tttgtccccc gttatccggt tctccggcct ggaaatagat accgaaaagc
     6541 gcaaagtgac actggacagg aaccgggttg aactctctcc caaagagttc gatctgatca
     6601 cgctcctggc atccaatccc ggcaaaagct ataaccgcaa acggctgtta aacctggtat
     6661 ggggatacga ttttgaaggt tacgagcata cggtgaattc ccatatcaac cggctaaggg
     6721 gaaagattga gaaagatatt tcaaacccga aatttattct gaccacctgg ggagtgggtt
     6781 accggtttaa tgaagaatta taaaatggaa ttatgtctct aaacacaagc cggggaaatc
     6841 ttttattctg gaaaatagcg gccgtattct cgggcctgct catcctcctg ggcttcatct
     6901 tcgtgctcat agcgtcccgg ttatccaggt cttattatac catggcccac caggaattgt
     6961 acggcaatgt ggcttcccac ctggcaacgt ttacgcatcc gctgaaaaac gggaaaccgg
     7021 atacggcggt cacgcatgac atcatccatt cgatcatggt ggccaacccc agtgttgagg
     7081 tttatctgct ggacactaat ggaaaaatta ccgactatgt ggtgccggat aaatctgtcc
     7141 ggaatcaccg ggtgaatatc agcaaagtga aagaatggct ggcggtcaaa ggcggggaac
     7201 ggccactggg agataatccc aaacagccgg atgagccggg tatcttttcg gctgcacccg
     7261 tttacgagaa cggacagctg acgggctatg tgtatgccgt tttggccagc gagaaacaaa
     7321 gggaaatcct ggcggcgttg aacagccagc tatatcaccg gttgggcata tacgttttct
     7381 tcgcagcgct attcattgcg tttatcgtgg gccttatcac ttttttcctg ataaccaata
     7441 gtattagtca ggtttcgggg gtag
//
LOCUS       DASYCU010000124         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4673269, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000124 DASYCU010000000
VERSION     DASYCU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4673269"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..>1766
                     /locus_tag="VGM24_02500"
     CDS             22..>1766
                     /locus_tag="VGM24_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEY4154258.1"
                     /translation="MKAEHARLDSYYLYFQSPKDKWFTENETNQQKVSGTPNESIFVK
                     DAFHDAVIRGENAEALRKIKKGTKFSPVYQFNIEGGKSQTIFLRLSNKSLGNPFTFGF
                     EKIFQQRKEEANAFYDEILEPQSDARLKLLQRQALSGLLWSKQYYHFDVEKWLTRSDG
                     ITAVNAGKLMGRNHDWKHLKNQDIVAMPDKWEYPWYAAWDMAFQCIPMSMVDPNFAKN
                     QLLLIMREWYMKPDGQLPAYEWNFSDVNPPVQAWAALQVYRLEKEKSGYADINFLKRI
                     FQKLLINFTWWVNRKDPSGNNMFEGGFLGLDNIGVFNRSIELPGKMKLEQADGTSWMG
                     MYALNMLDMALEIAMKDLTFEDTATKFFEQFVLIAEALNEQGMWNEEDRFFYDTLSIA
                     GAAPVQLRIHSIVGITSLFAVSIIEKKKLDKLSDFKKRINWFEHHRLTHKKFWPNEEK
                     TDGETILLSLVPQERLVSLLIRLLNEEEFLSPGGIRALSKYHEKNPYSVTIDNINYTI
                     QYDPGDSTSNLFGGNSNWRGPVWMPINYLIIQSIRKYGLFYGNTLKVECPTGSGNYLN
                     LEEVADFLTQRVISL"
BASE COUNT          501 a          393 c          444 g          428 t
ORIGIN      
        1 agcctgcgga ataaaaattc tgtgaaagcg gaacatgcgc ggctggacag ctactatttg
       61 tatttccaat caccaaagga taaatggttc acggaaaatg aaaccaatca gcagaaagtt
      121 tccggaaccc cgaacgaatc catttttgta aaagatgcat ttcacgatgc ggttatccgc
      181 ggggaaaatg cagaagcgtt gagaaagata aaaaagggaa ccaagttttc tcccgtatat
      241 caattcaata ttgaaggggg aaaatcccag acaatttttc tccgcctctc aaataaatcc
      301 ctcggtaacc ccttcacgtt tggatttgaa aaaattttcc agcaacgcaa ggaagaggct
      361 aatgcttttt atgacgaaat actggaacct caaagcgatg cccgtctgaa gctgctgcag
      421 cggcaggcgc tctccggctt gctctggagc aaacaatact atcattttga tgtagagaaa
      481 tggctgacca ggagcgatgg gatcaccgcg gtaaatgcgg ggaaactgat gggcaggaat
      541 cacgactgga agcacctgaa aaaccaggac attgtcgcga tgccggataa gtgggaatat
      601 ccctggtacg ccgcttggga tatggccttc caatgcattc cgatgtccat ggtagacccc
      661 aattttgcaa aaaaccaatt gctgctcatc atgcgcgaat ggtatatgaa accggatggg
      721 caacttcccg cttatgaatg gaatttcagc gacgtgaatc cgccggtaca ggcctgggcg
      781 gcattgcagg tttaccggct ggaaaaggag aagtcggggt atgcggatat aaatttttta
      841 aaacggatct ttcaaaaact gctgatcaat tttacctggt gggtcaaccg gaaagatccg
      901 agcggcaaca atatgtttga aggcgggttc ctgggcctgg acaatatcgg ggtctttaac
      961 cggagcattg agctgcccgg aaagatgaag ctcgaacagg ccgatggcac cagctggatg
     1021 ggcatgtatg ccctgaatat gctggatatg gcattggaga ttgccatgaa ggatcttacg
     1081 tttgaagata cggccacaaa attttttgag cagttcgtgc tgattgcgga agcgctgaac
     1141 gaacagggca tgtggaatga ggaagacagg tttttctacg acaccctttc gattgccggg
     1201 gctgcaccgg ttcaacttcg tattcactcc attgtgggaa ttacttccct gtttgcggtt
     1261 tcgattattg aaaagaaaaa actcgacaag ctctcggatt tcaaaaagag gatcaactgg
     1321 tttgagcatc accggctgac gcataagaaa ttctggccca acgaagaaaa aacggatgga
     1381 gaaaccattc tcctgtcgct ggttccgcag gaacggctcg taagcctttt gatcaggttg
     1441 ctgaatgagg aggaattcct ttcacccggc ggtatcaggg cgctttcaaa ataccatgag
     1501 aaaaatccgt attccgtaac cattgacaat atcaattata ccattcagta cgatccggga
     1561 gattccacat ccaatctgtt tggcggcaat tcaaactggc gcggacccgt ctggatgccg
     1621 atcaactacc tgatcattca gtccatccgg aaatacggtc ttttttatgg aaataccctg
     1681 aaggtcgaat gtccgaccgg ttcgggcaat tatctcaacc tcgaagaagt ggccgatttt
     1741 ctaacccagc gggtgatcag tctttt
//
LOCUS       DASYCU010000125         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3367273, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000125 DASYCU010000000
VERSION     DASYCU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3367273"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VGM24_02505"
     CDS             complement(<1..1216)
                     /locus_tag="VGM24_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEY4154259.1"
                     /translation="MKRLSFLPLLFLVFVGFSQILQPASPDRIYGDLFTEVQMQHIFP
                     DGKTFVDCIPKRKPSEVMYDYGMQKGPNFDLKKFVDENFEIPQPVAANYKTDSTQDVT
                     THIKNLWTVLKRNPDKEVEGSSLLPLPYPYIVPGGRFREVYYWDSYFTMLGLKESGEV
                     EMIENMIRNFAYLIQNYGHIPNGNRTYYLGRSQPPFFSSMVELLAGIKGDSIYQTFLP
                     DMEKEYAFWMDGADKVKPGQAYRRVVKMKDGRLLNRYWDDSTSPRPESYREDFLTTEK
                     SGRDKIEMYQDLRASAESGIDFSSRWFADNKSITSILTTDILPVDLNCLLYHLEWTIA
                     KARLINGDQEGARLFREKALKRGELIDKYFWNKKMSFYTDYNYKTGMPRNNITPAGLY
                     PFCFIREHPDYLS"
     gene            complement(1261..1476)
                     /locus_tag="VGM24_02510"
     CDS             complement(1261..1476)
                     /locus_tag="VGM24_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154260.1"
                     /translation="MNPTGATGIVPAYHVWDWSLSWHFLKYYHLSGGLNNVANAKYFT
                     RRINMYPGPGILPADGRSFYVSFGVKI"
     gene            complement(1619..2536)
                     /locus_tag="VGM24_02515"
     CDS             complement(1619..2536)
                     /locus_tag="VGM24_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154261.1"
                     /translation="MALTTSQKIHYTLRVGTALCFIGHGSFGIITKAVWCNYFAVFGI
                     GTRLAYQLMPWLGSFDILLGLIMLILPLRIIPAWLFIWGLITAACRPLSGEPVAEFFE
                     RAGNFGVPFTLLVLSGGLTKANLFSTIDPDLPADDKTLRRVFLFLKVIVFMVLTGHGI
                     LNLMDKKSLLDQYGSLGFSNPAKVALTVGLFEITAAVSVLIRPVRSLVLLFFVWKMGT
                     ELFYPHYEFFEWVERGGSYSALLALWFAIGANRLTRTAKDINSMEEGRPGSIFPGLIA
                     RTSYIVRSAFSTALHSAIHRTRSASGVKW"
     gene            complement(2642..3727)
                     /locus_tag="VGM24_02520"
     CDS             complement(2642..3727)
                     /locus_tag="VGM24_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154262.1"
                     /translation="MKKYFTVTICLILFFAARSLAQGCIAIRSTGGTSMLAHPEKDDE
                     KAWTFSASARYFKSFRHFSGTEENKSRQTQGSEVINHQASLDLAILRTLSNRWSFLLD
                     VPVLANSRSSLYEHGGKDRGVTHSFGIGDVRFAAYAWLFDPKKSRGNIQLGLGIKLPT
                     GDYDYKDYWTNIGPDKTRELRDVDQSIQLGDGGTGFTVEVNAFYHLTPALTVYGDGFY
                     LLNPREQNGARTYRETLSPTLANEAICSVPDQYMMRAGFAYSIAAIKGFSVAGGVKLE
                     GIPVNDLVGGSGDFRRPGYIWSAEPTLNYSTKNINFYLSVPVAFYRNRTQSVTDKENS
                     VTQGKHVQGDAAFADYVINAGFSIPLK"
     gene            complement(3929..>4454)
                     /locus_tag="VGM24_02525"
     CDS             complement(3929..>4454)
                     /locus_tag="VGM24_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154263.1"
                     /translation="LLFNWVGYQLYTSIMEQRADKTLIANLDENNYSESDLISIKVPT
                     VHFSYYVNSKDFERVDGKIEIEGVQYNYVKRRLFNDSLELLCIPNKTATQLKTAREEF
                     FKLVNDLQHPGHARKADQHTASFKCFNGEYYSDQHQFDLEARTSSSPKTSDHFLVQIP
                     SVYLSPAGQPPDFA"
BASE COUNT         1147 a         1038 c         1116 g         1153 t
ORIGIN      
        1 aactcaggta gtcgggatgt tccctgatga agcaaaacgg ataaagccct gccggggtaa
       61 tattatttct cggcatcccc gttttataat tgtaatcggt atagaacgac atctttttgt
      121 tccagaaata tttatcgatc agttctcccc tttttaaagc tttttcccgg aacagccggg
      181 cgccctcctg gtcgccgtta atgagtctgg cttttgcgat ggtccattcc aggtgataca
      241 gcaggcaatt caggtctacc ggcagaatat ccgttgtaag gatgctggta atacttttat
      301 tgtctgcaaa ccacctgctg ctgaaatcaa ttccgctttc tgcactcgcg cgtaaatcct
      361 ggtacatttc aattttgtcc cgtccggatt tttctgtggt gagaaaatct tcgcgatagc
      421 tttcgggacg cggagaagtg ctgtcgtccc agtatctgtt aagcagtctg ccatccttca
      481 ttttaacgac gcgccggtaa gcctgtccgg gtttaacctt atcggctcca tccatccaga
      541 atgcatattc tttttccata tccggaagaa aggtttgata gatactgtct ccctttattc
      601 cggccagcag ttcaaccatg gaggaaaaaa acggcggctg cgatctgccc aggtaatagg
      661 tccggttccc attgggaata tgaccatagt tctggatgag gtaggcgaaa ttgcggatca
      721 tattttcgat catttccact tccccgcttt ctttgaggcc cagcatggta aaataagaat
      781 cccagtaata tacttcgcga aaacggcctc cgggaacaat atacgggtat ggcaggggaa
      841 gcagggaact gccttccacc tctttgtccg ggtttctttt gagcacggtc caaagatttt
      901 ttatgtgggt ggtcacatcc tgcgtactgt ctgttttgta attcgcggca accggctgtg
      961 gaatctcaaa gttctcgtcg acaaattttt tcagatcgaa attgggtcct ttttgcattc
     1021 cgtaatcata catcacttcg gaaggctttc gtttggggat acagtcgaca aatgtttttc
     1081 cgtcagggaa aatatgctgc atctggacct cggtaaaaag atcaccgtaa atacgatccg
     1141 gggaggccgg ctgcaggatc tggctgaacc cgacaaaaac caggaaaagc agcgggagga
     1201 aggatagacg cttcatcggg gaatgtttgt tttaaagata acattttacc cccaaccgca
     1261 ttaaatcttc actccgaatg aaacataaaa agaccgcccg tcagcgggca ggatgcccgg
     1321 gccggggtac atgttgatcc ggcgggtaaa atatttggca ttggctacat tgttcaggcc
     1381 gccggacagg tgataatatt tcaaaaaatg ccagctcagg gaccagtccc atacatgata
     1441 ggcgggcacg atcccggttg ccccggtggg attcacccgg gtgttattgg catcgctgaa
     1501 gccctttccg gtaaagctgt actggaggct ggtgattcat gatttaatta ttttaatgca
     1561 gtaagaaaaa gaacccctgc gaagaatcgc agggatttgt attgcttatt actattgatt
     1621 accattttac gccggatgcg gaacgggtcc ggtgtatcgc tgaatgaagg gctgtggaaa
     1681 aagctgacct gacgatataa ctggttcttg ctataagccc cgggaaaatg ctccccggtc
     1741 tgccttcctc catggagttg atgtctttcg cggtccgggt taaacggttc gcgccgatcg
     1801 caaaccataa ggccagcaaa gcggagtaac tgccgccccg ttccacccat tcaaaaaatt
     1861 catagtgcgg ataaaaaagt tctgtgccca ttttccagac aaaaaagagg aggaccagcg
     1921 accggaccgg gcggatcagc acggatacgg ctgctgtaat ttcaaacagc cccacggtga
     1981 gcgccacttt tgccggattt gaaaaaccca gggaaccata ttgatcgagc aggctttttt
     2041 tatccatcag gttcagtatg ccatgcccgg tcaacaccat aaagacaatt actttcaaaa
     2101 aaagaaatac ccgccgcagg gttttatcat ctgccggtaa atcaggatct atcgtactga
     2161 agagattggc ttttgttaaa ccgccggaga gcacaagaag ggtaaagggc actccgaaat
     2221 tacctgccct ttcaaagaat tccgcgaccg gttcaccgga gagggggcgg caggctgccg
     2281 taataagacc ccagataaag agccaggccg gtataatacg aaggggaagg atgagcataa
     2341 tcagtcccag caggatatcg aaggatccca gccagggcat cagctggtag gccagcctgg
     2401 tcccgattcc gaaaacggca aaataattac accagaccgc tttggtgatt attccaaagg
     2461 agccatgccc gataaaacat aacgcggtgc caacccggag ggtataatgt attttctgac
     2521 ttgttgtaag tgccattacg ttcagtttat tttaagctct taaaaagaca gcacgaagaa
     2581 tcgggccgtc tggtttgctt tgtaccatag aaaaataatt agaaagtgct ggttataact
     2641 tttatttcag ggggatcgaa aagccggcgt tgatcacgta gtcggcaaat gcggcatcgc
     2701 cctgaacatg ttttccctgg gttacggaat tctctttatc ggtaacgctc tgcgtacggt
     2761 tgcggtaaaa agccaccgga acggagagat agaagttgat atttttcgta ctgtaattca
     2821 gggtgggctc ggctgaccag atatagcccg gcctgcggaa atcgccgctt ccgcccacca
     2881 gatcattaac cggaatacct tcgagtttta cgccgccggc tacagaaaat ccttttattg
     2941 ccgctatgga ataagcgaat ccggctctca tcatgtactg atccggcaca ctgcaaatgg
     3001 cttcattggc cagggtaggg gaaagggtct cgcggtaggt gcgggcgcca ttctgttcgc
     3061 gtggattcag caggtaaaac ccgtcgccat aaacagtcaa agcaggcgtg aggtggtaga
     3121 acgcatttac ttccacggtg aatccggttc caccgtcgcc cagctgaatg gactgatcca
     3181 catcccgtaa ttccctggtt ttatccggac caatatttgt ccagtaatct ttataatcgt
     3241 aatccccggt cggcagttta atgcccagac cgagctggat gttcccgcgg ctctttttgg
     3301 gatcaaatag ccaggcataa gcagcaaacc ggacatcgcc gataccaaaa gaatgggtca
     3361 ccccgcgatc cttccctccg tgttcgtaca gggaagaccg ggaattagcg agaaccggta
     3421 cgtccagcag aaaggaccat cggttgctga gggtgcggag gatggccagg tcaaggcttg
     3481 cctgatgatt gatcacctca ctaccctgtg tttgcctgct tttgttttct tctgttcctg
     3541 aaaaatgccg gaaagatttg aagtagcgcg cggacgcact gaaagtccat gctttttcat
     3601 cgtctttttc cggatgggcc agcatgcttg tgccaccggt agaccggatg gcgatacagc
     3661 cctgggcaag gcttctggct gcaaaaaaca gaatcaggca aatggttact gtaaaatact
     3721 ttttcataga cttgttttaa gctgaaagtc agtctggttt tttaataatt gtgtttacgg
     3781 gccggatcgg tctatccgtc cgttggtgaa actcccggtt gctattgcgt tacggcaaca
     3841 acgactggat aagatgatga taaaatggtt tgctgaaacg tattgcttca ttcacccgga
     3901 acgggtaaaa acggtacaat aaaatgtact aggcaaaatc cggaggctgt ccggcgggac
     3961 tgagatatac agaagggatc tgtactaaga aatgatcgct tgttttcggg gatgaggagg
     4021 tcctggcttc caggtcaaac tgatgctgat cggaataata ttccccgtta aagcatttga
     4081 aagaggccgt atgctgatcg gctttcctgg catgacccgg atgctgcagg tcattcacca
     4141 gtttaaaaaa ttcttccctg gctgttttaa gctgggtagc cgttttattg ggaatgcaga
     4201 gaagttccag tgaatcattg aagaggcggc gtttgacata attatattga acgccctcaa
     4261 tttcaatctt gccgtccacg cgttcgaaat ctttggaatt tacataatag gaaaaatgaa
     4321 cggtgggaac tttaatggag atcaggtcgg attcggaata gttgttttca tccagattgg
     4381 cgatcagcgt cttatccgca cgctgctcca tgatggaagt gtacaactga tacccaaccc
     4441 aattaaacag gagt
//
LOCUS       DASYCU010000126         8785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4959115, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000126 DASYCU010000000
VERSION     DASYCU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4959115"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VGM24_02530"
     CDS             complement(<1..940)
                     /locus_tag="VGM24_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154264.1"
                     /translation="MQKNLRISHNYFLIGLGLISLLAYTLLLCAFGLIRGYPISFWQF
                     PAAVVLMGITQFFASKYVFGKTATAPFFRSVGILAGIAVVSIIFARTIYDSSFNGQRY
                     QQELVNQLKTGFNPYLRTMPVPEEEMVSAPADSCCSATVQSPNEAGPAAVPPADPLYL
                     NINHSPKGIAIVEAAIYRFTDRIETGKAVNLMVLVASFCLGLSLLYKLNRFSAAKKWL
                     ITALAVFNPVSIAQLSTYATDGILYSVMLCLFLLFCFCLTEINKYYLYLLGLLIMISV
                     NIKFISLLYAGIFFAGFLLILIFQKNWLLCKRIFYAG"
     gene            1054..1950
                     /locus_tag="VGM24_02535"
     CDS             1054..1950
                     /locus_tag="VGM24_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4154265.1"
                     /translation="MKCILWPAFLLLSLHVRAQKTDHRLQKQIEQLLTGFNGHIGVYV
                     HDLKKDRVAELNGDSLFPTASVIKIPILIGICRRIQLQELDYQQEMIYKDTLNYDPGE
                     DILASFKPEEKIRLSKLMLLSITLSDNTASLLLQGLAGGGTRINGLMDSLGLKYTRVN
                     SRTAGREQDRNKYGWGQTTPREIASLMERIAFGKIFSSRLSERMLRILGRQYWDENAL
                     SQIPANVFVASKSGAVDESRNEVLYVNGPHPYVFSIFTKDNKDQRWDYNNEACVLTRR
                     LSALLWKYFNPAPDWTGVPVKN"
     gene            complement(1960..4281)
                     /locus_tag="VGM24_02540"
     CDS             complement(1960..4281)
                     /locus_tag="VGM24_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY4154266.1"
                     /translation="MKRQIIWSLFFAFPFYAMAQRWKKLAPARGEITLIRNRGGQTLG
                     YSGKSGVKIIIADGYAFKDLNKNGKLDPYEDWRLPADTRAKDLASKMSVEQIAGLMLY
                     SRHQPIPAAVNGPFAGTYHGKPFPQSGAAASDLSDQQIEFLTRDNVRHVLITSVQNAA
                     VAAQWNNNEQELAESMGLGIPANNSSDPRNGTVASAEYNAGAGGSISMWPGSLGMAAS
                     FDPELVRQFGHIAAREYRALGISTALSPQIDLATDPRWMRVSGTFGEDPELSTDMARA
                     YIDGFQTSSGRKEISGGWGYESVNAMVKHWPGGGTGEGGRDAHYAYGKYAVYPGNNFD
                     RLLDPFTQGAFKLEGKTGMAAAVMPYYTISFHQDTKRGDNVGNNYNAYIINDLLRNKY
                     HFDGVVCTDWQVTGDETAIDQFLSGKSWGVENLSIPERHYRILMAGVDQFGGNNDAAP
                     VMEAYRMGVKEHGEAFMRDRFEVSAIRLLRNIFRVGLFENPYLDTEISKKTVGKADYM
                     RAGYEAQLKSIVLLKNKSQVLPLTKNKTVYLPKRYTPAGRNFLGMETPEKLDYPVNRE
                     IVEKYFRVTDNPDEADYALVFISNPNSGGGYSREDVKSGGNGYLPISLQYGEYTAKEA
                     RDSSLAGGDPLENFKNRSYKGKSVKAINITDLAMVTDTYGKMKGKPVIVSINLSNPMV
                     FSEFEKDANAILVNFGVQDQAILDILTGSREPSALLPLQMPLDMNTVEKQAEDLPLDM
                     KCYQDSEGNSYDFGFGLNWKGVIQDKRVVTYKK"
     gene            4439..5254
                     /locus_tag="VGM24_02545"
     CDS             4439..5254
                     /locus_tag="VGM24_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154267.1"
                     /translation="MRIKTGFYPLVLFTAVSCVTPRAYFMSPADINTNSYHTLPMVAD
                     SQKSASYADLVFNTGGSNQELEDIVLGGRLDLHRAHQFGKFQAYYGAGLSLGNYQVAD
                     AYHFSNTYSGTGNDTAYHFRKADKFYGICGLNGGINLVIPFGNGRGEWRAIGLETSFQ
                     KEFGDYLRYRKGIPDSVVDVLATYDRMFTLGGMTEIVGKTRHGTEFGFKMAWGGILFP
                     RGNYRGDESHDRPFYFYNTLHITKKRVTGFLQVNIGDHAASFMFGVNYRISKK"
     gene            5307..5909
                     /locus_tag="VGM24_02550"
     CDS             5307..5909
                     /locus_tag="VGM24_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154268.1"
                     /translation="MKKLFFPAILSLTVAVTMGQPAFNDSLAQSRNRITEKAMLTLGS
                     WATANIAAGFIIASQTQGEAKYVWRMNAYWNFINLGLAGMGYLNARKAMGKKYDLAGN
                     LAAQQGIEKLYVFNLGLDLVYITGGFYLRERGNSETKPDHRDLFKGYGTSIAIQGGFL
                     LLMDGVLIALHHKNGIRLNNQVRRIELNAGPGGLGMVYHF"
     gene            complement(5906..7012)
                     /locus_tag="VGM24_02555"
     CDS             complement(5906..7012)
                     /locus_tag="VGM24_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HEY4154269.1"
                     /translation="MEFRSWYEIDQVDKIDSPALCIYKDRIRENIRRLLENVPAQQVR
                     PHVKTNKTAEVCRMMMEAGISKFKCATIAEAEMLGMTGAKDVLLAYSPTGPKISRFLR
                     LIKKYPDARFSCLVDSAEGARAVSSAFNNENRIADIFIDLNVGMNRTGISPDRAMALY
                     ETIRNLPGIRLTGLHAYDGHIHEKDFDERKAACIEAFREVPALKKAMETLAGYSISLV
                     AGGSPSYLIHARQGDRDCSPGTFIFWDKGYKDGLPEQPFDYAALLLCRIISIPAPNTL
                     CVDLGHKSVAAENPQPRVFFLNAPDAIPTAQSEEHLTLTVTDTGAYQIGQVLYGVPRH
                     ICPSVALYDKALVIEKHQLVETWKVIARQREISV"
     gene            complement(7022..7483)
                     /locus_tag="VGM24_02560"
     CDS             complement(7022..7483)
                     /locus_tag="VGM24_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4154270.1"
                     /translation="MTPEENFKNLGLELPPAPAPSGVYVPLLIDGRHCYVSGHGPVLP
                     DRKMLHGKLGAGLSMDDGKLAARQVGLTILSTLKSHLGSLDKIKRVIKVLGMVNADIS
                     FEKHPYVINGCSELFAQVWGKENGIGVRSAVGMGSLPDNIPVEIEALFELV"
     gene            complement(7494..>8785)
                     /locus_tag="VGM24_02565"
     CDS             complement(7494..>8785)
                     /locus_tag="VGM24_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEY4154271.1"
                     /translation="LLLILLISWLKLNTFLSFLIVSLGIGFANGMSVTAITDSVQKGM
                     GDLLGSLLVILVVGAMLGKLIADSGAAQRIVSALMKRFGMKYIVWALVITAFIVGIPL
                     FYGIGFVLMVPLVITLSYKYDIPAVYIGLPMLASLSVTHGYLPPHPSPVALIRQYHAD
                     LGLTLLYGFIAAVPAIIIAGPVFALTLKKYKQKPLATFAGKPIAEEDLPGLADSLITA
                     LLPVVLIAGATIVNLSGAPQNFLVRVITALGDPIVALLLTLIYALYALVLKKGKKLGP
                     CMVSLGDAVRDISMILLIIAGAGALKEILTQSGTADQIAAAMQGFSKYPILASWGIAA
                     IIRVCLGSATVAGLTTAGIMAPMLSASTVNPSLIVLATGAGSLMFSHVNDSGFWMFKE
                     YFNLSIKDTIRTWSMMETIVSVVGLFAVLGISFFVSH"
BASE COUNT         2206 a         2209 c         2237 g         2133 t
ORIGIN      
        1 aaccggcgta aaaaattctt ttacacaaca gccagttttt ttgaaagatc agaatcagga
       61 gaaagccggc aaaaaaaatg ccggcataca gcaatgaaat gaacttgatg tttacgctga
      121 tcatgatcag caaacccaat aagtacaggt aatatttatt gatctccgtc aggcagaaac
      181 aaaagagcag aaaaaggcaa agcataacgg aatacaggat accgtccgta gcatacgtgc
      241 tgagttgcgc gatgctgacg ggattgaaaa cggcgagggc cgtgatcagc catttctttg
      301 cagcggaaaa gcggttgagc ttatataaaa gagaaagacc caggcaaaag gaggcaacta
      361 aaaccatcag gttgacggcc tttccggttt cgatccggtc agtgaagcgg taaattgcag
      421 cttccacgat cgcaatccct ttcggggaat gattaatatt cagataaagc gggtctgccg
      481 gcggaacggc ggcagggccg gcctcattcg gtgactgaac cgtagcggaa cagcaggaat
      541 ccgccggtgc ggagaccatt tcctcttccg gaacgggcat ggttctcagg tagggattaa
      601 aacctgtttt taactggttg accaactcct gctggtaccg ctggccatta aaagaactgt
      661 cataaatggt cctggcaaaa ataatggaga ccacggcgat ccccgcaaga atgcctacgg
      721 atctgaaaaa gggcgcggtg gcggttttgc caaatacata tttcgaagca aaaaactggg
      781 taatccccat caggactacg gctgccggga attgccagaa tgaaatggga tagccgcgga
      841 taagaccgaa ggcacagagg aggagggtat aagccagcag gctgatcagc ccaaggccga
      901 ttaaaaaata attatgggag atgcggagat tcttttgcaa agcggatcgg aagttgggat
      961 tttgaaatca ggtaaagaat tgtttcttat ctgcaaatta ggcatttcat tcccgactct
     1021 ccattatcca tatctttatt gaaatggtcc atcatgaaat gcatactttg gccggcgttc
     1081 ctcttacttt ctttacatgt ccgggcacaa aagaccgatc accgcctgca aaagcaaatc
     1141 gaacagctac tgaccggttt caacgggcat atcggggtgt acgtgcacga tctgaaaaaa
     1201 gaccgcgttg cggagctgaa cggggacagc ctttttccca cggccagcgt gataaaaatt
     1261 cctatcctga tcggtatctg ccgcaggata cagcttcagg agctggacta tcagcaggaa
     1321 atgatttata aagacacgct taactacgac ccgggagaag atatcctggc ttcttttaaa
     1381 ccggaggaaa agatccgtct gagtaaactg atgctgcttt ccattacctt aagcgacaat
     1441 accgccagcc ttttactgca gggactcgcc ggaggaggaa cacgcataaa tggactgatg
     1501 gatagccttg gccttaaata tacccgggtc aacagccgca cggccggacg cgaacaggac
     1561 cggaacaaat acggatgggg gcagacgacc ccgcgcgaaa tagcttccct gatggaacgg
     1621 attgccttcg ggaaaatatt cagctcccgg cttagcgaac ggatgcttcg gattttagga
     1681 cggcaatact gggatgagaa cgcgctttcc cagattccag ccaatgtttt tgtcgcttcc
     1741 aaaagtggcg cggtggatga aagccggaat gaagtgctct acgtgaacgg gccgcatccc
     1801 tatgttttca gcattttcac caaggacaat aaagaccagc gctgggacta taacaatgaa
     1861 gcctgcgtgc tgacgcgtag attgtccgcc ctgctttgga aatatttcaa ccccgccccg
     1921 gattggaccg gagtgccggt gaagaactga tgggcgccct cactttttat aggtgaccac
     1981 gcgtttgtcc tgaataactc ccttccagtt caatccgaaa ccgaaatcgt agctgttccc
     2041 ttccgagtcc tgatagcact tcatatcgag cggcagatct tccgcctgtt tttcaaccgt
     2101 gttcatgtca agaggcatct gaagcggcaa caaggcggaa ggttcccggc tgccggtgag
     2161 tatgtccagg atggcctggt cttgtactcc gaaattcacc agaatggcat ttgcgtcttt
     2221 ttcaaattcg gaaaaaacca tggggttcga cagattgatg gaaacgatca ctggtttccc
     2281 tttcatcttc ccgtaggtat cggtcaccat ggccagatcg gttatattaa tggctttgac
     2341 cgatttccct ttatagctgc ggttcttaaa attttccagc ggatcgcccc cggccaggct
     2401 gctgtcccgc gcttctttgg ctgtgtattc cccatattgc aaactgatgg gcaggtatcc
     2461 gttaccgccg gattttacat cttctcggct ataaccgccg ccactgttcg ggttgctgat
     2521 gaataccagc gcgtagtctg cctcatccgg attatcggtc acccggaaat atttttctac
     2581 tatttcccgg ttcacggggt agtccagttt ttccggtgtt tccataccca gaaaatttct
     2641 cccggcgggg gtatatcttt tcgggagata gaccgtttta ttttttgtca gcgggagaac
     2701 ctgcgattta tttttcagca aaacaattga ctttaactgg gcttcgtagc cggcacgcat
     2761 gtaatctgct ttcccgactg tttttttcga tatttcggtg tccaggtatg gattttcaaa
     2821 aaggcctacg cggaaaatat ttctcaagag gcggatggcc gatacttcaa aacggtcacg
     2881 cataaacgct tctccatgtt ctttaacgcc catccggtac gcttccataa ccggagcggc
     2941 atcgttattc cccccaaact ggtccacgcc cgccatcagg atccggtaat gcctttccgg
     3001 gatggagaga ttttccacgc cccaggattt tccggaaaga aactggtcaa tggccgtttc
     3061 atcgccggtc acctgccagt ccgtgcagac cacgccgtca aaatgatatt tatttctaag
     3121 caggtcatta atgatatagg cattgtaatt atttcctacg ttatctcccc gtttggtatc
     3181 ctgatgaaaa gaaatcgtgt agtagggcat aacggcggcc gccatgccgg tcttcccttc
     3241 cagtttaaac gcaccctggg tgaacggatc caggagccgg tcaaaattat tgccgggata
     3301 aacggcatat ttcccgtagg cgtaatgggc atccctgcct ccttctcccg ttcctccccc
     3361 gggccagtgc ttgaccatcg catttacact ttcataaccc catccgccgg aaatttcctt
     3421 tcttccggaa gaagtctgaa aaccgtcaat ataggcgcgg gccatatccg tggacagttc
     3481 cggatcttcc ccaaacgtgc cgctaacccg catccagcgc ggatccgtgg cgaggtcgat
     3541 ctggggggaa agggctgtgc tgatgcccag tgcgcggtat tcccgggccg caatatggcc
     3601 gaactgccta accagttccg gatcaaagct ggccgccatt cccagggatc cgggccacat
     3661 ggaaatggat cctcctgcac cggcattgta ttctgccgat gccacggttc cgttccgggg
     3721 atccgaactg ttatttgcgg gaatgcccaa tcccatcgat tcggccagtt cctgttcgtt
     3781 attattccat tgcgctgcca ctgccgcatt ttgaacggaa gtgatcagca catgacgaac
     3841 attgtcccgg gtcaggaatt caatctgctg atcggagaga tcggatgccg ccgcaccact
     3901 ctgcggaaaa ggttttccat ggtaagtgcc ggcaaaaggt ccgttgaccg cagccggaat
     3961 cggctgatgc cggctgtaca gcatcagacc ggcgatctgt tccacagaca ttttcgaagc
     4021 aaggtccttt gccctcgtat ctgccggtaa acgccagtct tcgtaaggat ccagtttccc
     4081 gtttttattc aggtccttga aggcgtatcc gtcggctata ataattttta ccccggattt
     4141 gccggaatag cccagggttt ggcctccccg gtttctgata agggttattt cgcccctggc
     4201 aggcgccagc tttttccatc tttgggccat tgcataaaag ggaaacgcaa agaacagcga
     4261 ccagatgatc tgccttttca tattttaccc gattttttct tctttaaagt tattgtttta
     4321 ggtgctctga aagaatatct tttatcaggc gttggatttg tttattcttt aacagaaaaa
     4381 acgcagcagt attcaataaa atttgccttt atcgggataa accacagcct gctgctccat
     4441 gcgaatcaaa accggttttt acccgcttgt cctgtttact gccgtttcct gcgtaacgcc
     4501 ccgcgcctat ttcatgagcc cggcagatat caatacgaac agctatcata ccttgccgat
     4561 ggtcgctgat tcgcagaaat cggccagcta tgcagacctt gtttttaata ccggcggctc
     4621 taaccaggaa ttggaagaca tcgtcctggg cggaagactg gatcttcacc gggcccacca
     4681 gttcggcaaa ttccaggctt attatggtgc cgggttgagt ttgggaaatt atcaggtggc
     4741 tgatgcgtat catttttcaa atacctattc cggcacaggg aacgacacgg cctatcattt
     4801 ccgcaaggcg gataaattct atggaatctg tggtttaaac ggcggtatca acctggtcat
     4861 tcccttcgga aatggcaggg gagaatggcg ggcgattggt ttggaaacct cttttcaaaa
     4921 agagtttggc gattacctcc gttaccggaa agggatcccg gattccgtgg tagacgttct
     4981 ggccacctac gaccggatgt tcacccttgg cggaatgacg gaaatagtgg gaaaaacccg
     5041 tcacggtaca gaatttggat ttaagatggc atggggcgga atcctttttc cccggggaaa
     5101 ttaccgggga gatgaaagcc atgaccgtcc gttttatttt tataatacct tgcatataac
     5161 aaaaaaaagg gttacgggat ttttacaggt caatataggc gaccatgcgg ccagctttat
     5221 gtttggtgtg aattaccgga taagtaagaa ataacgatgc cggcggaaag atcgtcccgg
     5281 aaaacaattc aaatcgtctt taaagcatga aaaaattatt ttttccggct atcctgagcc
     5341 tgacggttgc ggtgactatg ggccaaccgg catttaacga cagcctggca caatccagga
     5401 accgcatcac ggagaaagcc atgctcactt tgggctcctg ggccacggcc aatatcgcgg
     5461 ccggatttat cattgcatca caaacgcagg gagaagcgaa atatgtatgg agaatgaacg
     5521 cttactggaa ttttatcaac ctgggcctgg ccggtatggg atatctcaat gcgcgtaaag
     5581 ccatgggaaa gaaatatgat ttggcaggta acctcgccgc gcagcagggt attgaaaaat
     5641 tatatgtttt taatctgggg ctcgacctcg tttatatcac cggcgggttt tatttgcggg
     5701 agcggggaaa ttcagaaaca aaacccgatc accgcgatct gttcaagggt tatggaacca
     5761 gtatcgccat ccagggcgga tttcttttgc tgatggacgg ggtgttgatc gcgcttcatc
     5821 acaagaacgg tatccggtta aataatcagg tccgccggat cgaattaaat gcgggcccgg
     5881 gcggattggg aatggtctat catttttaaa cgctgatctc ccgttgccgg gcaatgacct
     5941 tccaggtttc cacaagctga tgcttttcta ttaccagggc tttgtcgtag agagcaacgg
     6001 aggggcaaat atggcgggga acgccatata atacctggcc gatctgatac gcacccgtgt
     6061 ccgtgaccgt tagcgtcagg tgctcttcgc tttgtgcagt gggtatggca tccggcgcat
     6121 tcagaaaaaa aacgcggggt tgcggatttt cagccgcaac cgatttatgc ccgagatcca
     6181 cacaaagcgt atttggagcc ggaatggaaa tgatgcggca caagagcaga gcggcatagt
     6241 caaacggctg ttccggaagc ccatccttat agcccttgtc ccagaaaata aaagttcccg
     6301 ggctgcagtc ccggtcaccc tggcgggcat gaataagata ggagggggaa ccgccggcaa
     6361 ccagggaaat ggaatagcct gcgagggttt ccatcgcttt tttcaaagcg ggaacttccc
     6421 ggaaggcttc gatgcatgct gctttccgtt cgtcgaaatc cttttcgtga atatgtccgt
     6481 cataggcatg caatcccgtc agccgtattc cgggcaggtt ccggatcgtt tcatacaaag
     6541 ccatcgcgcg gtcaggcgaa atgccggtgc ggttcatgcc cacattgagg tcaataaaaa
     6601 tatcagcgat ccggttttcg ttattgaacg cggaagagac cgccctggcg ccttccgcgg
     6661 aatccaccag gcaggagaaa cgggcgtccg gatatttttt tatcaatcgg aggaagcggc
     6721 tgatcttcgg accggtaggg gaataagcca gcaacacatc ttttgctccg gtcatcccca
     6781 gcatttctgc ttctgcaatg gtggcgcatt tgaattttga tatcccggct tccatcatca
     6841 tccggcagac ttcggcggtt ttattggtct ttacatgcgg ccggacctgc tgcgccggaa
     6901 cgttttccag caaccggcgg atattctccc ggatcctgtc tttataaata cacagcgcgg
     6961 gggaatctat tttgtccact tgatctattt cataccagga gcggaattcc ataagggaaa
     7021 attaaaccag ttcaaacaaa gcttcaattt caacagggat attatccggc agcgaaccca
     7081 tgcccaccgc gcttctcacc ccgatcccgt tttcttttcc ccagacctgg gcaaacaatt
     7141 cgctgcatcc gttgatcaca taaggatgtt tttcaaagga aatatcggca ttcaccatcc
     7201 ccagcacttt aatcacgcgt tttattttat cgaggcttcc cagatgtgat tttaaagtgg
     7261 agagtatggt aagtcccacc tgcctggcag ccagctttcc atcgtccata ctcaaaccgg
     7321 ctcccagttt gccgtggagc attttccggt ccggaagaac ggggccgtgt ccggaaacat
     7381 aacaatgcct gccgtcaatc agaagcggaa cataaacccc cgagggggcc ggggccgggg
     7441 gtaattcgag gccgagattt ttaaaatttt cttccggggt catattcata tttttaatgg
     7501 ctgacaaaaa aactgattcc caaaacagca aataatccaa cgacggatac aatggtttcc
     7561 atcatactcc aggtgcggat ggtatccttg atgctcaggt tgaaatattc tttaaacatc
     7621 cagaatcccg aatcgttcac gtgggagaac attaaactcc ccgctccggt agccagcacg
     7681 atcaggctgg ggtttacggt gctggcggac agcatgggcg ccatgatgcc ggccgtggtg
     7741 agccccgcca cggtggcgga tcccaggcaa acccggatga tcgcggcaat accccaggaa
     7801 gcgaggatgg ggtatttgct gaatccctgc atggctgccg caatctgatc agccgtgccg
     7861 ctttgcgtca ggatctcttt caatgcgccc gctccggcga tgatcagcag gatcattgaa
     7921 atatccctga ccgcatctcc aagggaaacc atgcaggggc ctaatttttt ccccttcttc
     7981 agcacgagcg catagagcgc gtagatcagg gtgagcagca gagccacgat cgggtcgccc
     8041 agtgcggtga tcacccggac cagaaaattt tgaggcgcgc cggataagtt aacgatggtg
     8101 gcgccggcaa tcagtacgac gggcagcaaa gccgtgatca ggctatctgc caggccgggc
     8161 aggtcttcct cggcgattgg ttttccggca aaggtggcca gcggtttttg tttgtatttt
     8221 ttaagggtca gggcaaaaac cggcccggca ataatgattg ccggaacggc tgcaatgaac
     8281 ccgtatagca aggtaaggcc aagatctgcg tggtactgcc tgatcagggc aaccggggaa
     8341 gggtgaggcg gaaggtaacc gtgagtaacc gataaagagg ccagcatggg aagcccgata
     8401 tagaccgccg gaatatcata cttataagaa agggtgatca ccaggggaac catcaggaca
     8461 aaaccgattc cgtaaaacag gggaatacct acgatgaagg cggtgattac cagggcccac
     8521 acaatatatt tcatcccaaa cctcttcatg agagccgaca caatacgctg ggctgccccg
     8581 ctgtcggcga tcagtttacc cagcatggcg cccaccacca ggatcaccag cagggatccc
     8641 agtaaatcgc ccatcccttt ttgtaccgaa tccgttatgg ccgttacgga catgccattg
     8701 gcaaacccga tacccagcga cacgatcaga aaagacaaaa atgtatttaa tttaagccag
     8761 ctgatcaaaa gaatgagtaa gagaa
//
LOCUS       DASYCU010000127         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_177829, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000127 DASYCU010000000
VERSION     DASYCU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177829"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..998
                     /locus_tag="VGM24_02570"
     CDS             57..998
                     /locus_tag="VGM24_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4154272.1"
                     /translation="MQYSEINNRRLSKLTLGTVALGKDYGISNKSGKPALTDSREILS
                     SALNAGINFIDTANDYGNAEQIIGEYLEKYKNGKQVNVVTKFKISKANLLNKERARAE
                     AFRSVRSSLQLLKTKRVAICLFHKSMDQPVEQVLEILPSLFSDLINDGLADTCGVSVF
                     HPDELNTFLSHPVLQAFQSPMNLFDQRLIKNGALKKLNEEKKILFIRSVFLQGLFFMS
                     PDELTGNLAQASEYIRTLQGISTESGMSIAQLAFSYLRDLDGISSLVFGAVAPEQVMQ
                     NAGLLNGPALSRELRLQIEKRFADVPEEIITPALWSL"
     gene            1025..1813
                     /locus_tag="VGM24_02575"
     CDS             1025..1813
                     /locus_tag="VGM24_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4154273.1"
                     /translation="MDIFRLDGKIAIVTGGAGYYGKPICLALAEAGARVIIASRDVEK
                     CREYAATLKNRYLKADGMFLDLADDQSINELVSRVYQEYGKIDILVNNAVSRHGFKDL
                     EQISRDEFENSQRINSTGLLLITQAAIRVMREQRSGNIINIGSIQGVVGPNFPVYGDT
                     GMTSPVNYTCDKWAMVGFTKWIANYYGRFNIRANCISPGGYGPGVAETIGDNEFVQNY
                     KRLTPLGRFAEDDDIKGPVVFLASAASAYITGHNLLVDGGWTSW"
     gene            1863..>2540
                     /locus_tag="VGM24_02580"
     CDS             1863..>2540
                     /locus_tag="VGM24_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4154274.1"
                     /translation="MSRQNTFTADSGSTADQAELKPDARAWLVVALLCIVGTLNYLDR
                     IMITTMRSSIIDAMPMTDAHFGLLTSVFLWVYGMLSPFAGFLADRFNRSRVIIVSLFV
                     WSAVTVLTSYASTFQELLITRALMGVSEACYIPAALALIADYHKGSTRSLATGIHMSG
                     IMAGQSLGFLGGWIAENHNWNYSFSILGIIGIIYSVILSFTLRDGPRIVNRASVAESK
                     LDFFRAVR"
BASE COUNT          687 a          572 c          660 g          621 t
ORIGIN      
        1 cgggagtaag gaccaacgcg gttacctcat taagagatga actaaaataa cagagcatgc
       61 agtattcgga aataaacaac cggcgcctat caaaactcac actgggaacg gtggcactcg
      121 gaaaggatta cggaatctcc aataagagcg gcaagcccgc attgacagac agccgggaaa
      181 tactttcttc cgcgctgaat gcaggcatta attttattga cacggccaac gactacggaa
      241 atgccgaaca gataatcggt gaatacctgg agaaatataa gaatggaaag caggtgaacg
      301 tagttacaaa attcaagatc agcaaggcaa acctgttgaa caaagaacgg gcaagggcgg
      361 aagcattcag gagtgtgcgg tcttcccttc aattgctaaa gacaaaacgg gttgctatat
      421 gcctgtttca taagtcaatg gatcagcccg tagagcaggt gctggaaatt ctcccctcgc
      481 tgttttccga cctgataaat gacggactcg cagatacctg cggggtgtcg gtttttcatc
      541 cggatgaatt aaatacgttt ctgagccatc cggtattgca ggcatttcaa tcccccatga
      601 acctgtttga tcagcgatta ataaaaaacg gggcgttaaa aaagctaaat gaagagaaga
      661 aaatattatt tatccgcagt gttttcctgc agggactttt ttttatgtcg cccgacgaat
      721 tgaccggaaa cctggcccag gcatcagaat atatcaggac cttacagggt atctcaacag
      781 aatcgggaat gagcatagcc cagctcgcct tttcctattt aagggatctg gacggcatca
      841 gcagcctggt ttttggagca gttgcgccgg agcaggtaat gcaaaatgcc ggtctcttaa
      901 atggaccggc cttaagccgg gagctgagat tgcagataga aaagcgattt gcggatgttc
      961 ccgaagaaat catcacaccg gctttgtggt ctttataaat attcgcttaa ttaaaacata
     1021 tgcaatggat atattcagac tggacggtaa aatagccatt gtgaccggcg gggcgggtta
     1081 ctacggcaaa ccgatctgcc tggcgctcgc agaagccggc gcccgggtga tcattgcttc
     1141 gagggatgtg gagaagtgca gggaatatgc ggcgacactg aagaatagat atctgaaagc
     1201 agatggaatg ttcctggatc ttgcggacga tcaatcgata aatgaactgg tttcccgggt
     1261 ttatcaggaa tatggaaaaa ttgatatcct ggtaaacaac gccgtatcgc ggcacggatt
     1321 taaagatctc gaacagatca gccgggacga atttgagaat tcgcaacgta taaactccac
     1381 cggtcttctc ctcataactc aagccgccat cagggtcatg cgcgaacagc gctccgggaa
     1441 cataatcaat atcggatcca ttcagggcgt cgtgggaccc aattttccgg tttacgggga
     1501 taccggcatg accagtcccg taaattatac ctgcgataaa tgggcaatgg tcggattcac
     1561 aaaatggatt gcaaattatt atggcaggtt caatatccgc gccaactgca tcagtcccgg
     1621 agggtatgga ccgggggtgg ccgaaaccat tggagacaat gaatttgttc agaattataa
     1681 aagactgacg cccctgggac gatttgctga ggatgacgat attaagggcc cggtggtttt
     1741 ccttgcttca gctgcctctg cctatattac aggccataac ctcctggtgg acggaggttg
     1801 gaccagctgg taaggattgt ctgctcccgg aagaaaatga aaatgtaaat caaaaacagg
     1861 gaatgagcag gcaaaatact tttaccgctg atagcggatc aacggcagac caggcggaat
     1921 taaaacccga tgcgcgcgcc tggctggtag ttgccctcct ttgtattgtg ggcacgctga
     1981 attatctgga ccggatcatg atcaccacga tgcgcagttc gatcattgac gcaatgccaa
     2041 tgaccgatgc gcatttcggt ctgctcacct cggtatttct ttgggtctat ggaatgctga
     2101 gcccgtttgc gggttttctc gcagaccggt ttaatcgcag ccgggttata attgtcagct
     2161 tatttgtatg gtcggccgta acggtgctca cttcttatgc atccactttt caggaattgc
     2221 tgatcacgcg tgcgctgatg ggagtcagtg aagcctgtta tattcccgca gcgctggcgc
     2281 ttattgcgga ttatcacaaa gggtcaacgc gctctttggc caccggtatt catatgtcgg
     2341 gcatcatggc aggccaaagc ctgggcttcc ttggcggatg gatcgcggaa aaccacaact
     2401 ggaattattc cttcagtatt ttgggaatta tcggcatcat ttattcggtc attctttcct
     2461 ttaccctgcg tgacggcccg cggattgtaa atagagcatc cgttgcggaa agcaaactgg
     2521 actttttcag agcggtcagg
//
LOCUS       DASYCU010000128         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2350758, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000128 DASYCU010000000
VERSION     DASYCU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350758"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..575)
                     /locus_tag="VGM24_02585"
     CDS             complement(24..575)
                     /locus_tag="VGM24_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4154275.1"
                     /translation="MAEQITDSELLALFREPDTKEKAFSILVKKYQERLYWHIRRMLV
                     DHEDSNDVMQNVFIRVWNALANFREDAQLYTWLYRIATNECLTFIEQQKRKATVSIND
                     LESGLSNKIRADKDFDANKLEWKLQLAIQQLPEKQRVVFSLRYYDEMPYEEMSQVLET
                     SAGALKASYHHAAKKIEDFILNH"
     gene            complement(615..1565)
                     /locus_tag="VGM24_02590"
     CDS             complement(615..1565)
                     /locus_tag="VGM24_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4154276.1"
                     /translation="MITVTGQKDTRSGFGDGIVEIARKNPDIVALTADLAGSLKLNQF
                     IKEFPDRFIQCGIAEANMISIAAGMTIGGKIPYTTTFANFSTGRVYDQIRQSVAYSGK
                     NVKICASHAGLTLGEDGATHQILEDIGMMKMVPGMTVIVPCDYAQTKAATMAIADYSG
                     PVYLRFGRPVWPIFTSEMEFKIGKAQVLTEGKDVSIFACGHLVWIALEAEKILKEKGI
                     SAEIINIHTIKPLDSEAILKSIRKTGCAVSAEEHNILGGLGDSIAQTAAKNFPVPQEY
                     VGTNDTFGESGTPTQLLEKYGLDSAHIVAAAEKAIARNNK"
     gene            complement(1597..>2216)
                     /locus_tag="VGM24_02595"
     CDS             complement(1597..>2216)
                     /locus_tag="VGM24_02595"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY4154277.1"
                     /translation="AESPYGYTKQMGEQIINEFYKSNDLNAILLRYFNPVGAHPSGII
                     GELPIGRPANLVPAITQTAAGKLPQMQVYGDDYPTRDGSCIRDFIHVCDIAHAHTLSL
                     QFLIEGKETKKCEVFNLGSGNGVTVLEAIHAFEKISGMPLNYSIGPRRPGDVIAIYAN
                     NDLARKKLGWNPKFSLEDMLATAWRWEQKIKMDETFYNSKFSELN"
BASE COUNT          501 a          538 c          521 g          656 t
ORIGIN      
        1 agaccctttc agggcttcaa agtttaatga ttgagaataa aatcttcgat ttttttcgcc
       61 gcatggtgat aggaagcttt taaagccccc gcagaggttt ccagaacctg gctcatttcc
      121 tcataaggca tttcgtcata ataccgtaga ctaaacacaa ctcgctgttt ttcaggcaat
      181 tgctgaatgg ccagctgcag tttccactcc agtttattgg catcaaagtc tttgtcggcc
      241 cggatcttat tgctcaaccc gctttccagg tcattgatgg agacggtcgc tttccttttt
      301 tgttgctcga taaaagtcag gcattcattg gtcgctatcc ggtagagcca ggtataaagc
      361 tgtgcatcct cccggaagtt ggccagtgcg ttccagaccc ggataaatac attctgcatg
      421 acatcattgg aatcttcatg atccaccagc atgcgccgga tatgccaata gagacgttcc
      481 tgatatttct taaccaggat actgaatgct ttttcttttg tatccggttc gcggaacaaa
      541 gccaacaatt cactatccgt aatttgctcc gccatcagtt tgatcaggtt gttttataac
      601 tacggaatat ccggttattt attattccgg gcgatggctt tctcagctgc tgctacaata
      661 tgcgctgaat caagtccgta tttttccagc agctgggtgg gggtcccgct ctcgccaaaa
      721 gtatcattcg ttcctacata ttcctgtggg accgggaaat ttttggccgc tgtttgcgca
      781 atgctgtcgc ccagaccacc caggatattg tgttcttccg cggaaacagc acatcctgtt
      841 ttccggatgg atttcaagat cgcttcgctg tccaggggtt tgatggtatg aatatttatt
      901 atttctgcgg aaatgccttt ttccttcaat attttttccg cttccagggc aatccatacc
      961 agatgtccgc aggcaaaaat agaaacgtcc tttccttcgg tgaggacctg ggcctttccg
     1021 attttgaatt ccatttctga cgtaaaaatc ggccagaccg gtctgccgaa acgcaggtat
     1081 acgggtccgc tgtaatcggc gatggccatc gtcgcagctt ttgtctgcgc ataatcacag
     1141 ggcacgatca ccgtcatgcc gggtaccatc ttcatcatac cgatgtcttc cagaatctga
     1201 tgggtggcgc cgtcttcgcc aagggttaat ccggcatgtg aagcacagat cttcacattc
     1261 tttcctgaat aagccacgga ctggcggatc tgatcgtaaa cccttccggt ggagaaattt
     1321 gcaaacgtgg ttgtataggg aatttttccg ccgatggtca ttcccgcagc aatgctgatc
     1381 atattggctt ccgcgattcc gcattgaata aaccggtcag ggaattcttt gatgaactga
     1441 ttgagtttca gagaacccgc caggtcggcg gtgagtgcca cgatatcagg gttctttctg
     1501 gctatttcca caatgccgtc accaaagccg ctccgagtat ctttttgtcc ggttacagta
     1561 atcattttgt tgctgattgt ttatgtataa aagttatcaa ttcagttctg aaaacttgct
     1621 gttgtaaaaa gtttcgtcca ttttaatttt ttgctcccat cgccaggcgg tggccagcat
     1681 atcttccagc gaaaatttcg ggttccagcc aagctttttc cgggccaggt cattgttggc
     1741 gtaaatggcg attacgtctc cgggcctgcg cggaccgatc gagtaattca gcggcatccc
     1801 gcttattttt tcaaaagcgt gaatggcttc caggaccgtt accccgttcc cgctgcccag
     1861 gttaaaaacc tcgcattttt ttgtttcctt tccttcgatc aggaattgga gagaaagggt
     1921 atgtgcatgg gcaatatcgc atacatggat aaagtcgcgg atgcaggagc catcgcgcgt
     1981 gggatagtca tcgccataaa cctgcatctg gggcagtttt cccgcagcgg tttgggtaat
     2041 tgccggaacg aggttggccg gccggccaat cggaagttca ccgatgattc cggatgggtg
     2101 tgcacccacc ggattaaagt atctcagcag aatggcattt aaatcgttgg atttgtaaaa
     2161 ttcattaatg atctgttccc ccatctgttt ggtatatccg taaggagatt cggccg
//
LOCUS       DASYCU010000129         2994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3658066, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000129 DASYCU010000000
VERSION     DASYCU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658066"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /gene="tyrS"
                     /locus_tag="VGM24_02600"
     CDS             complement(<1..756)
                     /gene="tyrS"
                     /locus_tag="VGM24_02600"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY4154278.1"
                     /translation="MSLLDELKWRGMIQDIIPGTAEQLSKESSSGYIGFDPTADSLHI
                     GSLVPILLLVHLQKAGHRPVVLVGGATGMVGDPSGKSEERNLLDEETLQKNLTGIRTQ
                     LEKYLDFSRHIPNAAILVNNYDWFREIHFLDFIRDAGKHITVNYMMAKDSVKKRLEGA
                     SGMSFTEFSYQLIQGYDFYWLYKNMNCKLQMGGSDQWGNILTGAELIRRKTGGEAFAF
                     TCPLLTKSDGGKFGKTEAGNVWLDPEKTSPYRFY"
     gene            847..1992
                     /locus_tag="VGM24_02605"
     CDS             847..1992
                     /locus_tag="VGM24_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEY4154279.1"
                     /translation="MVLTSVVRRTKRYCSLPTLLLLLLSKPATAQFAKYSNEFLNIGA
                     GARGMSMGNAQVASVSDGTAGYWNPAALADIRTSPQLNLMHAEYFAGIGKYDYANLVL
                     PLKDNKRTLGLTLLRFAVDDIPNTVFLVQPDGSIDFSNITTFSSADYAFLISLAQQVN
                     WIKNRKVNFGINAKIIHRQAGDFTKAWGFGFDAAVQVTGNRWKLGLVAKDVTTTFNAW
                     SYNINQEWREVFYATQNDIPSNSIELTAPQLIPGFSYNFPITQKINLLAEADFDITFD
                     GRRNTVLSFDPVSIDPRMGLELSYNNVFFVRGGINNFQKALDDRDTTNRQKVWIYQPS
                     IGAGFRIGDVQIDYAFTNLANQSNPLYTHVFSLKVDLVDTKKKSKKN"
     gene            2021..>2994
                     /locus_tag="VGM24_02610"
     CDS             2021..>2994
                     /locus_tag="VGM24_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154280.1"
                     /translation="MKKIFTLCLLLCAGRLVAQTYGNEWIDPGKTYYKFKLAADGVYR
                     IPQSVIAAAGLGNSDVKDFQLFRNGVEVPIYTSAGSGLLAPGDYIEFWGQMNDGKPDK
                     PLYRSPLYQHTDKWSLETDTATYFLTVDPTGSTFHYKTVLNDSSTNTLPVEPYFMYTA
                     GTYFRNQINPGTAAVLEEYIYSSAYDKGEFWSSDFSTPGNPVSDNQDNLYIFNGGPAA
                     NLKFGAAGCSDTLRRIQVKVNNLLVKDTTMNSFDDLVSNVPLPLAQLTSASNNFQFIN
                     NSQAVTYADRLVVSFYELTYPRQFNFGMRANFSFELPARVSGYFLKIS"
BASE COUNT          756 a          756 c          705 g          777 t
ORIGIN      
        1 ataaaaccgg tagggagaag ttttttcagg atcaagccag acatttccgg cttcggtttt
       61 cccaaatttg cccccatccg atttggtaag cagggggcag gtaaaagcaa aagcctcgcc
      121 acccgttttc cggcggatca gttctgcacc cgttaatata tttccccatt gatcgcttcc
      181 ccccatttgc agtttacaat tcatattctt atacaaccaa tagaaatcgt acccctggat
      241 gagctggtag ctgaattctg tgaagctcat gccggacgcg ccttccagtc ttttctttac
      301 actatccttc gccatcatgt aattcacggt aatgtgcttt cccgcatccc ggatgaaatc
      361 caggaaatga atttccctga accagtcgta attattgacg aggatcgccg cattggggat
      421 atgcctgctg aaatccagat atttttccag ctgagtccgg attccggtaa ggttcttttg
      481 cagggtttct tcatccagca ggttcctttc ctcgcttttt ccgctgggat cgcctaccat
      541 gccggtggcg ccgcccacca ggaccaccgg tcggtgaccg gctttctgca ggtgaaccag
      601 taataaaatg gggaccaggc tgccgatatg gagactgtct gcggtcgggt caaatccaat
      661 atatccggag ctggactctt tggaaagctg ctccgctgta ccgggtatga tgtcctggat
      721 cataccccgc catttcaatt catctaacaa cgacataact gttctgggga gttttggcaa
      781 aagtaaggga tattcgcatc ctgaacaggt cgcaataatc tgaataaaaa cgcgttagaa
      841 tagtttatgg tattgacttc agtcgtacgc aggaccaaaa gatactgctc ccttcccacc
      901 ctcttgctct tgcttctcag caagccggca acggcccaat ttgcgaagta ttccaatgaa
      961 tttttgaata ttggcgcggg ggccaggggt atgtctatgg gcaatgccca ggtagcttcg
     1021 gtgagcgacg gaacggccgg atactggaat cctgctgccc tggcggatat caggaccagt
     1081 ccgcagctga acctgatgca cgcagagtat tttgccggga tagggaaata tgattacgcc
     1141 aacctggttc ttcccttaaa agataataaa cggaccctgg gcctgaccct gctccgtttt
     1201 gccgtggatg atattcccaa tacggtattc ctggttcagc cggacggaag catcgatttc
     1261 agcaatatca ccacgttttc ctccgcggat tacgcttttc tgatctccct ggcccagcag
     1321 gtaaactgga tcaaaaacag gaaagtaaac ttcggcatca atgccaagat tattcaccgc
     1381 caggcagggg attttacgaa agcctggggg tttgggtttg atgcggccgt ccaggtaacc
     1441 ggaaaccgct ggaaactggg ccttgtcgcc aaagacgtga caaccacatt caatgcgtgg
     1501 tcgtataata ttaatcagga gtggcgggaa gtattttatg ccacgcagaa cgatatacct
     1561 tccaattcca ttgaattgac cgctccgcaa ctgattccgg gattctccta taatttcccg
     1621 atcacccaaa agatcaattt actggctgag gctgattttg atattacgtt tgatggtcgc
     1681 cgtaatacgg ttttaagttt tgatcccgtt agcatcgacc ccaggatggg attggaatta
     1741 tcctataata atgttttttt tgtaagaggg ggaatcaata attttcaaaa ggccctggat
     1801 gaccgggata ccacaaaccg gcaaaaggtc tggatttacc agccttccat cggagcgggt
     1861 ttccggatcg gagatgtgca gatagattac gcgttcacca acctcgccaa ccagtcgaac
     1921 cccttgtata cgcacgtgtt ttcattaaaa gtcgatctgg tggatacgaa aaagaaaagt
     1981 aaaaagaatt aacccccacc gtgggaaacc gaaccaaact atgaaaaaga tttttacact
     2041 gtgtttgctc ctttgtgccg gcaggttggt cgcccaaacc tacggtaacg aatggataga
     2101 ccccggtaaa acctactata aattcaagtt ggcggccgac ggggtttacc gtattcccca
     2161 gtcggtgata gctgcagccg gactgggaaa ctccgacgtt aaagattttc agctcttccg
     2221 caacggggtg gaagtgccca tttatacctc cgccggcagc gggctgctgg cgcccggaga
     2281 ttatattgaa ttctggggtc agatgaatga cggaaaaccg gataaacccc tttatcgcag
     2341 tccgctttat cagcatacgg ataaatggag cctggagacc gatacggcaa cttatttcct
     2401 taccgtggac cccaccggtt ccactttcca ttataaaacc gtcctgaacg acagcagcac
     2461 gaataccctg ccggtggaac cttattttat gtacactgcc ggaacctatt tccggaatca
     2521 gataaatccc ggaacggccg cggtactgga agaatatatt tattcctccg cttacgataa
     2581 gggagaattc tggagctctg atttctccac ccccggcaac ccggtttcgg ataaccagga
     2641 taacctgtat attttcaacg gcggccctgc cgccaacctg aaattcggcg cagcaggctg
     2701 ttccgatacc ttgagaagga tacaggtaaa agttaataat ctcctggtca aggacaccac
     2761 aatgaatagt ttcgatgacc tcgtcagcaa tgtaccgctt ccgcttgccc agctgaccag
     2821 cgcttccaac aattttcagt ttattaataa ttcccaggct gtcacgtacg ccgaccggct
     2881 ggtcgtttct ttttatgaac tgacttatcc gagacagttc aattttggaa tgcgggcaaa
     2941 tttctccttt gaattgccgg cgagggtcag cggatatttt ttaaagatca gcaa
//
LOCUS       DASYCU010000130         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4237541, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000130 DASYCU010000000
VERSION     DASYCU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4237541"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2491
                     /locus_tag="VGM24_02615"
     CDS             <1..>2491
                     /locus_tag="VGM24_02615"
                     /inference="COORDINATES: protein motif:HMM:NF026036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY4154281.1"
                     /translation="SYSPDGKDMALVFVTQAFRNWKRYRGGWKANIHIYHFAGNTSED
                     ISANEVAGDEFPMWSGHFIYFLSDRGKEVRMNLWRYDVSDRKMEQLTHFTDYDIHFPS
                     LGPEEIVFEEGGKLCLFSLATQQVTEVKVTVVTDRSTLKPKMVSAEKFVQHAAISPDG
                     KRALIEARGEVFSLPARDGFVKDLTRTTGAGERYPAWSPDGKTLAYWSDQSGEYELWV
                     VPADGEGTPKKISRYGPGFRYRLTWSPDSRKLAFIDQAMKIKIIDIASGNTTEVDQAL
                     RFMDGGCENFNCSWSPDSRWLTYSRDLDNYHNAVFLFDTRNSKLQQVTDGFYSCDNPV
                     FDSEGKYLYLTTSQSFTPYYSDLDNTFIYGNSTRLAAISLKKTTPSLLYPKNDTVSAV
                     SEESKTQDNKKEDKKKDSSKNKVAEEKKPGAAPVDIDLDGLETRMILLPPPAGNYGKI
                     SSAKGKIIYLRLPVTGTKDGHPSLHYFDISKREEKNILENVNDYWLSSNTEKILVQRE
                     KAFVVISPEESQKFEKPLPLADMQMMVDPVLEWKQIFNDAWRLERDYFYDPGMHGVDW
                     NLVKERYSKMLEGAITREEVNFIIGEMLGELNSSHTYHGGGDLEKEPRENVGYLGVDW
                     LADGNYYKIKTILKAAPWDAEVHAALDVPGLDIKEGDYILAVNGVPISTAEEPFAFFQ
                     GLAKKTVELTYNTKPSLAGAKTAIVQTLDDEYRLRYLAWVEANRKKVDQETNGEAGYI
                     YVPSTGLDGQEELTRQFDAQWNKKALVIDERFNSGGQIPDRFIEMLNRTPLAFWATRD
                     GAVWPWPYAAHFGPKVMLINGWSGSGGDAFPD"
BASE COUNT          677 a          590 c          694 g          530 t
ORIGIN      
        1 tcctattcac cggatgggaa agatatggcg ctggtatttg ttacgcaggc attcaggaac
       61 tggaaaagat accgcggcgg atggaaagcg aatattcata tttatcattt tgccggcaat
      121 acctcggaag acatttccgc aaacgaagtg gcgggagatg agttccccat gtggtccggc
      181 cattttattt atttcttatc cgatcgcgga aaggaagtcc gcatgaacct ctggcggtat
      241 gatgtcagcg ataggaaaat ggaacagctc acgcacttca ccgattatga tattcatttc
      301 ccctcgctcg gtccggaaga gattgttttt gaagaagggg gaaaattatg cctgttctcc
      361 ctggccaccc agcaggttac ggaggtgaaa gtgaccgttg ttacggaccg gtccacctta
      421 aaaccgaaga tggtttcggc tgaaaaattc gttcagcatg cagcgatcag cccggatgga
      481 aaaagggccc tgatcgaagc gaggggcgag gtcttctccc tgcctgcgcg ggatggattt
      541 gtcaaggacc ttaccagaac gacaggagcg ggagaacgtt atccggcctg gtcgccggat
      601 ggaaaaacac ttgcttactg gagcgatcag tccggcgagt atgaactctg ggtagttccg
      661 gcggacgggg aaggaactcc aaaaaagatc agccggtacg gcccgggatt ccggtaccgg
      721 ctgacctggt cgccggacag caggaagctg gccttcatcg accaggcgat gaaaataaaa
      781 attatcgata tcgcttccgg caacacgacg gaggtggacc aggccctgcg tttcatggat
      841 ggcggctgcg agaatttcaa ctgcagctgg tcgccggaca gccgctggct cacgtacagc
      901 cgtgacctgg acaattatca caatgccgtt tttcttttcg atacccggaa cagcaaattg
      961 cagcaggtca cggacggctt ctacagctgc gataatccgg tattcgattc agaagggaaa
     1021 tacctgtacc tgaccacgag tcagtcgttc accccgtatt acagcgacct ggacaatacg
     1081 tttatttacg gcaactccac ccggctggcc gcaatcagcc tgaaaaagac cacgccttct
     1141 ttattgtatc cgaagaatga taccgtttcg gccgtttccg aagaatcaaa gacccaggat
     1201 aataaaaagg aggataaaaa gaaagacagc agcaaaaaca aagtggctga ggagaaaaaa
     1261 ccgggtgccg cgccggtaga tattgacctc gacgggctgg aaacccggat gatccttttg
     1321 ccgcccccgg caggtaatta tggaaaaata agttctgcca aaggaaaaat catttacctg
     1381 agattaccgg tgaccggaac gaaagatggt catccctccc ttcattattt tgatatttcc
     1441 aaacgggaag aaaaaaatat cctggagaat gtgaacgatt attggttgag cagcaatacg
     1501 gaaaagatac tggttcaaag ggagaaagcg tttgtggtaa taagcccgga ggaatcccag
     1561 aaattcgaaa aaccgcttcc cctggcggac atgcagatga tggtggatcc ggtcctggaa
     1621 tggaaacaga tcttcaacga tgcgtggagg ctggaacggg attattttta tgatccgggc
     1681 atgcacgggg ttgactggaa cctggtgaaa gaaagatatt cgaaaatgct ggagggcgcc
     1741 attacccgcg aggaagtgaa ttttataatc ggtgaaatgc tgggtgaact caattcatcc
     1801 cacacctatc acggcggagg cgatctggaa aaagagccca gggaaaatgt gggctacctg
     1861 ggtgttgact ggcttgctga cgggaattac tataaaataa aaacgatcct gaaggctgcg
     1921 ccctgggatg ctgaagtgca tgccgccctg gatgtgcccg gtctggatat caaagaagga
     1981 gattatattc ttgccgttaa cggagtgccc atcagcacgg cggaagagcc gtttgctttt
     2041 ttccagggac tggcgaaaaa gaccgtggag ctcacctaca atacaaaacc ttctctggcc
     2101 ggcgccaaaa cagcgatcgt tcaaaccctg gatgacgaat accgtttgcg ctacctggcc
     2161 tgggtggaag ccaacagaaa aaaagtggat caggaaacaa acggggaagc cggttatatt
     2221 tatgtgccca gcaccgggct cgacggacag gaagaactca cgcggcagtt cgatgcccaa
     2281 tggaacaaaa aagcgctggt catcgatgaa cggtttaata gcgggggtca gattcccgac
     2341 cgctttatag aaatgctgaa ccgtacgccg ctggcctttt gggcaacccg cgacggggcg
     2401 gtatggccct ggccctacgc cgctcatttc ggaccgaaag tgatgctcat taacggctgg
     2461 agcggttccg gaggcgacgc cttcccggat t
//
LOCUS       DASYCU010000131         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4237627, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000131 DASYCU010000000
VERSION     DASYCU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4237627"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..961)
                     /locus_tag="VGM24_02620"
     CDS             complement(149..961)
                     /locus_tag="VGM24_02620"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4154282.1"
                     /translation="MTIDAEVHFWKYKKTTQAPLIRENKMLREDQLPEQVSLNLRRNG
                     VEGCIAVAAEPAEVETRFLSELALTHPVIGAVIGWIDLKEKNAAKKIAELSSYTAIKG
                     FKLDDSAENFPAPENIMQTLARYGYALDLRVSPGTATARLNDRIRAFPDQHFILENAG
                     NPDTRIPPGAEWIRQIKQLSENQNLYCKVSGLFTLGDWKSWKPAALYLFLDILFECFG
                     PKRLLYASDWPFILLSGSYVQWKSLLEKYTEKLNEEEKENFFGENARRIYQL"
     gene            complement(1034..1465)
                     /locus_tag="VGM24_02625"
     CDS             complement(1034..1465)
                     /locus_tag="VGM24_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154283.1"
                     /translation="MKKVFLLAGLILSVFLTRAQRFFYLESNVLTEKFLKEGLLKASQ
                     FVTPSPLYSDYIIKTEAGFKPESNTTTLTITVQDSISFKTIFQADEKYSISGSAANAR
                     KVFSRVLEMLLYKDLHQMILSAKDNHSDGMVKMLKPGKDKT"
BASE COUNT          410 a          404 c          452 g          446 t
ORIGIN      
        1 gggttaaccg aagatgcgga aataatccag ggattgatgt tttgggtcag gccggaagaa
       61 agggagaaaa cggccattcc gaaggagatc agcagtctgg tgcagagcaa tcgctttttc
      121 atgaggtcaa gttaataata attggcattc agagctgata gatgcgacgg gcattttctc
      181 cgaagaaatt ttccttttct tcctcgttca gcttttccgt gtatttctcc agcaggcttt
      241 tccattgcac ataagaaccc gaaagaagga taaagggcca gtcgctggca tacaggagac
      301 gctttggtcc gaagcactca aacaggatat ccagaaagag atagagagcg gccggcttcc
      361 aggacttcca gtcgcccagg gtgaacaggc cggatacttt acaatacagg ttttgatttt
      421 ccgacagctg tttgatctgc cggatccatt ccgcgccggg gggaatcctc gtatccgggt
      481 ttcctgcgtt ttccagaatg aaatgctggt ccgggaaagc acggatccgg tcattcagcc
      541 gggcagttgc ggtacctggg gaaacccgga gatccaatgc ataaccatac cgggccaggg
      601 tttgcatgat attctccggc gcgggaaaat tttcggcgga gtcatccagc ttaaaccctt
      661 tgatagcggt ataggaggaa agttccgcta tttttttcgc ggcattcttt tccttcaggt
      721 ctatccagcc gatcacggcg ccgatgaccg gatgtgttaa agccagctcc gaaagaaaac
      781 gggtctccac ttcggccggt tcagcagcca cggcgatgca gccttccact ccgttgcgcc
      841 ggaggtttag cgagacctgc tccggaagct ggtcttcccg gagcatttta ttttcccgga
      901 tcaggggcgc ctgcgtcgtt tttttgtatt tccagaaatg aacttcggcg tcaatggtca
      961 tgaagaatat ttttttgcag gcccggtcgg gacccgctgc cggtttaaaa atactgagaa
     1021 gtatcgggcc ggttcaggtt ttatctttcc cgggcttaag cattttcacc atgccgtcgg
     1081 aatggttatc ctttgcggag aggatcatct ggtgaagatc tttgtaaagg agcatctcca
     1141 gcacccggct gaaaactttc cgggcatttg cggctgaccc ggatatgctg tatttttcat
     1201 ccgcctgaaa aatcgttttg aaactgatag aatcctgcac cgtgatggtc agggtggtgg
     1261 tgttcgactc cggtttgaat cccgcttcgg tcttaataat ataatcggag tagagcggag
     1321 agggcgtaac gaactgggag gctttcagaa gtccttcttt gaggaacttt tctgtcagaa
     1381 cattggattc cagataaaaa aaccgttgcg cccgggtcag gaaaacgctc aggatcaggc
     1441 ctgccagaag gaatactttc ttcatgatga tttggtaaat taaaagtagg aaatttattc
     1501 cggttccggc ggaaggtccc tgcggccacc cgttccaaaa ctaacactca ttagtttttt
     1561 aagctaaatt tgttgattat cagccgattt ctttatgaat acctcaacac aaaggctgag
     1621 gatgctgacc gcaacctccc tcttcgacgg gcatgatgcc gccatcaaca tcatgcgccg
     1681 gatcttccag agcaagggag tggaggtgat tc
//
LOCUS       DASYCU010000132         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4237891, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000132 DASYCU010000000
VERSION     DASYCU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4237891"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2321)
                     /locus_tag="VGM24_02630"
     CDS             complement(<1..2321)
                     /locus_tag="VGM24_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4154284.1"
                     /translation="MKERLKELSGQLAGELYTDDTMRILYATDASAYREMPLAVAIPK
                     SVDDLKTLIDFSRKNHSSLIPRTAGTSLAGQVVGNGIVVDVSKNFTRILELNKEEHWV
                     RVQPGVVRDELNMFLKPHGLFFGPETSTANRAMMGGMVGNNSCGSNSVVYGSTREHLL
                     EVRALLSDGSESVFSPLSADAFHEKCEGTNLEATIYKTIRTLLGNEATRQEITREFPK
                     KSIERRNTGYAVDELMDAAPFTAGRPDFNFCKLIAGSEGTLAFLTEIKLNLVPLPPSE
                     TGLLCVHFHTIDESLRANLIALKYKPSASELIDHYILECTRENIEQNKNRFFVKGEPG
                     AILVVEFRRSGRADITEIAKKLETDLRAAGLGYHFPLLFGEDSKKIWSLRKAGLGLLG
                     NMPGDEKAVPVIEDTAVDVNDLPQYISDFNQILEKYKLHAVHYAHAGSGEIHLRPIIN
                     LKTAEGNRLFRTIAEEIASLVKKYSGSLSGEHGDGRLRGEFIKQMVGNKNYELFKTIK
                     RTWDPENIFNPNKIVDTPPMNSMLRYMPGQKTPELKTVFRFNGQNILQHAEQCNGSGD
                     CRKSHLSGGTMCPSFMATRNEKDSTRARANILREFLTNSYKANRFDHQEIYEVMDLCL
                     SCKGCKSECPSNVDVAKLKAEFLQQYHDANGVPFRSRVIANFNRSARLGSLFPGVYNF
                     MVTNGVTGNLLKRLLGFAVKRSMPTLQKTSLRKWYGKRIPPAAANQRKVYLFCDEFTN
                     YNDTEIGIRAILLLEKLGYEVIIPKHEESGRAW"
     gene            2554..>3641
                     /locus_tag="VGM24_02635"
     CDS             2554..>3641
                     /locus_tag="VGM24_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEY4154285.1"
                     /translation="MISAGFVFQKAGIPAIATPGFESKRPPLSQRHFRSEAVEEVIAG
                     SKKWLPGRELGWLFENCFPDTLDTTVDFEMTGEGPDTYVITGDIDAMWLRDSSAQVWP
                     YLPLIRKDKKLQQLIAGVINRQTRCILLDPYANAFYKDESKVSEWKKTDITDMKPGIH
                     ERKWEIDSLCYPVRLAHKYWKTTGDVSPFDSHWKEAIRLIVKTFKEQQRKTGPGPYQF
                     ERNTAWATDGVPLGGYGYPVKPVGLICSMFRPSDDATIFPFLVPSNFFAVVSLRQAAE
                     MLNSISKDATLANECTQLANEVEQALKQYATATHPRSGKVYAYEVNGFGSFNLMDDAN
                     VPSLLSLPYLGAVPNTDPVYQNTRKLLFS"
BASE COUNT          864 a          896 c          904 g          977 t
ORIGIN      
        1 atccacgctc ttccgctttc ttcatgcttc gggataatca cttcatatcc cagtttttcc
       61 agcaaaagga tggccctgat gccgatttct gtgtcgttgt agttggtaaa ttcatcgcag
      121 aacaggtata cttttcgttg gttggcggct gccggaggaa ttctcttgcc gtaccatttt
      181 cgcaggctgg tcttctgcag cgtgggcatg gagcgcttca ctgcaaaacc gagcaggcgt
      241 ttcaggagat tgccggtaac cccgttcgtt accataaaat tataaactcc cggaaacagg
      301 gatcccagcc gcgccgaccg gttgaaatta gcaattaccc gggaacgaaa gggcacgcca
      361 ttggcgtcat gatattgctg caggaattct gctttcagtt tggccacatc cacattggaa
      421 gggcattctg atttgcagcc tttacaactc aggcaaagat ccatgacttc gtagatctcc
      481 tggtgatcga agcggtttgc cttataggaa ttggtcagga actctctcag gatattcgct
      541 ctggcccgcg tggaatcttt ctcattccgg gtagccatga aggaagggca catggtgccg
      601 cccgataaat gcgatttccg gcagtcgccc gaaccgttac actgttccgc atgctgtaaa
      661 atattctggc cgttaaatcg aaaaaccgtt ttgagttccg gcgttttctg tccgggcata
      721 taacgcaaca tgctattcat gggcggagta tccacgatct tgttggggtt aaaaatattt
      781 tccgggtccc aggtgcgctt aatggttttg aataattcgt agttcttatt gcctaccatt
      841 tgtttgatga attcgccccg gagccttcca tctccatgct ctccgctaag ggagccgcta
      901 tattttttca ccagggaggc aatttcttcg gcgatggttc tgaacagcct gttcccttcg
      961 gccgttttca gattgatgat gggacgcagg tggatttccc ccgatccggc atgcgcgtaa
     1021 tgtactgcgt gcagcttgta tttttcgagg atctgattga agtccgaaat atattgcggc
     1081 aggtcattta cgtccaccgc ggtgtcttcg atcaccggaa ctgctttttc atcgcccggc
     1141 atattgccca gcagacccag ccccgctttg cgcaggctcc atattttttt actgtcttcc
     1201 ccgaacagga ggggaaagtg ataacccagc ccggcagcgc gcagatctgt ttctaatttt
     1261 tttgcgattt cggtaatatc ggctcttccg gatcttctga attcaactac caggatggcg
     1321 ccgggctctc cctttacgaa gaagcggttt ttgttttgtt caatattttc cctggtgcat
     1381 tccaatatat aatgatcaat cagctcgctg gcgcttggtt tatattttaa ggcaatcagg
     1441 ttagccctca gcgattcatc aatcgtatgg aagtgaacgc aaagcaagcc cgtttcggaa
     1501 ggcggcaggg gaacgaggtt taatttgatt tccgtcagga aggccagggt gccctcggat
     1561 cccgcaatca gtttgcaaaa attgaaatcc ggtcttccgg cggtaaacgg tgcggcatcc
     1621 atcaattcat ccacggcata accggtattc cgcctttcga tggatttctt cggaaactcc
     1681 cgcgttattt cctgcctggt cgcttcattg ccgagcaggg ttctgattgt tttatagatg
     1741 gtcgcttcca gatttgttcc ttcgcatttt tcatggaacg catccgcgct gagcggactg
     1801 aatacggatt ccgagccgtc gctcagcaac gcacgaactt ccagcaggtg ttcccgggtg
     1861 cttccgtaca ccacagaatt ggagccacag gaattatttc ccaccatgcc gcccatcatg
     1921 gcgcggttgg cggtggacgt ttcgggacca aaaaataaac catgcggttt caggaacata
     1981 ttcagctcat cgcggacaac tcccggctga acccttaccc aatgttcttc tttattcaac
     2041 tccaggatgc gggtgaaatt cttggagaca tccaccacga tgccgttgcc gactacctgt
     2101 ccggccagtg aagttcctgc cgttcgcggg atcagggaac tgtgattctt tctggagaaa
     2161 tcaataagcg ttttcaggtc atccaccgat ttgggaatgg cgacagccag cggcatctcc
     2221 cggtaggcgg atgcatccgt ggcatacagg atcctcatgg tatcgtctgt gtataactca
     2281 cctgctaatt gcccggataa ttccttcagc ctttctttca tggggataat tgctgcgcaa
     2341 aattataaaa caatggtcgt ttattgaatt gtttttgatt ttattattat attccgttta
     2401 caaaacccaa aacgggtctg atgagaattc cgcatttatt tttcgaggtt gtcttaatcc
     2461 atcgcgggtt tctttgctgg tatacatgca aaccggaaga gttaaataaa tgacaatatg
     2521 ccgacacgca gaaaatttat aaaatcagcc ggtatgatat ctgccgggtt tgtttttcag
     2581 aaagcgggta ttccggcaat agccactccc ggttttgaaa gtaaacgacc cccgctatcc
     2641 cagcggcatt ttaggagcga agcggtcgaa gaagtgattg ccgggtctaa aaaatggttg
     2701 cccggaaggg agcttggctg gttatttgaa aactgttttc cggataccct ggacaccacg
     2761 gtggattttg aaatgaccgg agagggcccg gatacgtatg tgataacagg ggatatagat
     2821 gccatgtggc tgcgcgacag cagtgcgcag gtatggcctt atctcccgct gatccggaag
     2881 gacaaaaaac tgcagcaact gattgccggc gtgatcaacc ggcaaacccg ctgcattctc
     2941 ctggatccgt atgccaatgc attctataag gatgaatcga aggtcagtga gtggaaaaag
     3001 accgatataa cggatatgaa gccgggcatc cacgaaagga aatgggagat tgattcactc
     3061 tgttatccgg tcaggctggc acataaatac tggaagacaa cgggagatgt ttccccattc
     3121 gacagtcatt ggaaagaggc catccggctg atcgtcaaaa cattcaaaga acaacagcgc
     3181 aaaacaggtc cggggcccta tcagtttgaa cgcaatacgg catgggcaac ggatggtgtg
     3241 ccattgggcg gttatggata tccggtaaaa cctgtcggac tgatctgttc catgttccgg
     3301 ccaagcgacg acgcgaccat atttcctttc ctggtcccct ccaatttttt tgcggttgtt
     3361 tcgctcaggc aggctgcgga aatgctgaac agcatcagca aagatgcaac cctcgccaac
     3421 gaatgcacac aactggccaa tgaagtagag caggcgctaa agcaatatgc caccgcaacg
     3481 cacccccgat cggggaaagt atatgcttac gaagtaaacg gtttcggaag ttttaattta
     3541 atggatgatg ccaacgtgcc atcgctgttg tcgctgcctt atttgggagc agttcccaat
     3601 acggatccgg tttaccagaa cacaaggaaa ctgctttttt c
//
LOCUS       DASYCU010000133         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3802288, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000133 DASYCU010000000
VERSION     DASYCU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3802288"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VGM24_02640"
     CDS             complement(<1..594)
                     /locus_tag="VGM24_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4154286.1"
                     /translation="MKRTKTIRKLTVYFGVLLFGLLISSGGLAQNSNAVARNITGKIV
                     SKNGETVSGASITVKGTQNTVSSDARGEFSILAANGETLVISSVGFSPKEIRITNGTR
                     GLDVSLSDDYGNLQDVVVVGYGKMKKTDMSSSQVTVTADDISKTVNTTFDQALQGRAA
                     NVYVSFPSGQPGAAPSVIIRGLSSLTGSTQPLYVIDGV"
     gene            complement(629..>1956)
                     /locus_tag="VGM24_02645"
     CDS             complement(629..>1956)
                     /locus_tag="VGM24_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY4154287.1"
                     /translation="NVSDRQPDWKGYISTFYDAFNGDARKGFWDLINEKLYKKGVDAF
                     WMDASEPDILSNVSPERRKEEMTPTAIGPAAAYLNAYPLENTKGIYEGQRAADPGKRV
                     FILTRSAYAGSQHYGAAVWSGDIAARWSDMKTQITAGINFSLSGIPFWTMDIGGFAVE
                     HRYENAQGKDLEEWREQMTRWYQFGAFCPLFRVHGQFPFREIYNVAPEDHPAYQSMLY
                     YDKLRYRLLPYLYTLAARTYHDDYTIMRGLVMDFEKDTTAGHISDQYMLGPSLLINPV
                     YTFQARNREVYLPAGQGWFNFYSGTYESGGQKISADAPYGRMPVFVKEGSIIPFGPAL
                     QYTSEKPADPIDLFVYGGRDASFTLYEDENGNYNYEKGLFANIPISYNESTKTVTIGK
                     REGSFPGMLKERTFRISLITKNKPVELNFDADRAQTVHYNGNREIIKLK"
BASE COUNT          497 a          498 c          429 g          532 t
ORIGIN      
        1 cacaccatca atcacataca atggctgggt ggatcccgtt agtgaactca gtccccggat
       61 gatcacggaa ggtgctgccc ccggctgacc actgggaaaa gaaacataca cattggctgc
      121 gcgtccctgc aatgcctgat cgaatgtggt gttgactgtt tttgaaatgt cgtccgcagt
      181 aacggtaacc tgggaactgc tcatatctgt cttcttcatt ttaccatatc ccaccaccac
      241 gacatcctgc aagttgccat agtcgtccga aagggacaca tccagaccgc gcgtaccatt
      301 ggttattctg atctccttcg gagaaaatcc aacagaggaa ataacgaggg tttccccatt
      361 ggcagcaagg atggaaaatt ctccccgggc atccgagcta acggtgtttt gagttccttt
      421 taccgtgatg gaagcgccgg aaacagtttc accgtttttt gaaactattt tacctgttat
      481 attccgggca acagcatttg aattttgagc aagccctccg ctactgatca acagtccaaa
      541 taggagaacg ccaaaataaa cagtcagctt cctgatggtt ttggttcttt tcatataaaa
      601 acaattagtg tttaaaaaaa ttaaaatctt atttcagctt aattatttcc cggttgccat
      661 tgtagtgaac agtctgggcc cgatctgcat caaaatttaa ttcaaccggt ttattctttg
      721 tgatcaggct gatccggaaa gttctttctt tcaacatacc cggaaatgat ccttctctct
      781 taccgatcgt cactgttttt gtgctttcgt tgtaactgat gggaatattc gcaaacagcc
      841 ctttttcgta attgtaattg ccgttttcat cctcatataa agtgaaggag gcatcccggc
      901 cgccgtaaac aaataagtca atgggatcgg caggtttttc tgaagtatac tgaagcgcgg
      961 ggccgaatgg aatgatggaa ccctccttta caaacacggg cattcgcccg taaggggcgt
     1021 cggcagaaat tttttgtccc ccgctttcat aggtgcctga ataaaagtta aaccagccct
     1081 ggcctgccgg aagataaact tcgcggttgc gggcctggaa tgtataaacc gggttgatca
     1141 gcagggacgg tcccagcata tattggtcgc tgatatggcc ggctgtggta tctttttcaa
     1201 aatccatcac cagtccccgc ataatggtgt agtcatcatg ataggtcctg gccgccaggg
     1261 tatagagata gggcaacagg cggtaacgaa gtttatcata atagagcatg ctctgataag
     1321 ccggatggtc ttccggcgcc acattataaa tttcccggaa cggaaactgt ccatgtaccc
     1381 tgaataaagg acaaaatgca ccaaactgat accatctcgt catttgctcc cgccattctt
     1441 caagatcttt tccctgcgca ttttcgtagc ggtgttccac cgcaaaaccc ccgatatcca
     1501 ttgtccagaa agggattccg gataaagaaa aattgatccc tgccgtgatc tgggttttca
     1561 tatccgacca gcgggctgcg atatctccgc tccatacagc cgctccataa tgctgcgaac
     1621 cggcataggc tgaccgggtc agaataaata ctcttttacc cggatctgcg gctcgctgtc
     1681 cttcgtagat cccctttgta ttttccaggg gataagcatt taaatacgcc gccgccggac
     1741 cgatggcggt aggtgtcatt tcctcttttc ttctctccgg actaacattg gacaggatat
     1801 ccggctcgct ggcatccatc caaaaagcat ccactccttt tttatacaat ttctcattga
     1861 tcagatccca gaaccctttg cgcgcatccc cgttgaaagc atcgtaaaaa gtggagatat
     1921 accctttcca gtcaggctgc cggtcagaca cattcc
//
LOCUS       DASYCU010000134         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2789180, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000134 DASYCU010000000
VERSION     DASYCU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2789180"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..988)
                     /locus_tag="VGM24_02650"
     CDS             complement(101..988)
                     /locus_tag="VGM24_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4154288.1"
                     /translation="MKLSIKSLSKTYANKVQALKDVTLTLNNGMFGLLGPNGAGKSSL
                     MRTIATLQQADQGSIFLDDLDVLKNKTAVRQMLGYLPQEFGVYPKISAERMLDHIARL
                     KGIDHAGERRELVGALLENVNLSRDRKKHLGTYSGGMKQRFGIAQALIGDPKLIIVDE
                     PTAGLDPAERNRFYNLLSQLGENTIVILSTHIVEDVSTLCSNFAIICQGEVLYAGEPE
                     MAVKEMEGKIFSKAIGKEALGRYQKEYNVISTQLKSGKLHIRVLQETAPGNGFEPGSP
                     NLEDVYFSNIATRMDINTI"
     gene            complement(985..1737)
                     /locus_tag="VGM24_02655"
     CDS             complement(985..1737)
                     /locus_tag="VGM24_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154289.1"
                     /translation="MRTSNKFIFSALLLTLMALVVYDLLLKAEYRSGNYKIPYQNYVG
                     LNFRNFDVLDLNSSTVANVRLVQGPFSVRINENALEYVKVQQKGNHLQIDAGFEDQYY
                     GNPNPYLLVISCPKLTSLNADASYQTGDGPYTDSIAREDWNMRRVLIDGFIQDSLQIS
                     ADYGSTVVLANNHIRSLHAVIGKSRGSGSGLIIQNTNRFQDAVLDIRHKSKLQLENAM
                     IDNLHYDLGDSAQLLVSGKAQNLLSNSKPDQK"
     gene            complement(1721..2125)
                     /locus_tag="VGM24_02660"
     CDS             complement(1721..2125)
                     /locus_tag="VGM24_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4154290.1"
                     /translation="MEFRDNEAIYLQIVAYVSKNILLDKWPAEQKIPSVRDLAIELQV
                     NLNTVMRSYEYLENLGVIYNKRGLGSFVAADGYDKVKAYRKERFIRQDLPVFFTNIYL
                     LGIGPEEIAQWYEQFKNNNHELKPKSEDENKQ"
     gene            complement(2141..>2502)
                     /locus_tag="VGM24_02665"
     CDS             complement(2141..>2502)
                     /locus_tag="VGM24_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154291.1"
                     /translation="FNIFKRNPLEMALTYAFLHSIAFFGAVFFERLHFIKTAFVFFVS
                     LTLLIILNKMIQGALLGRAVEAAPPFGYLRFSENGQVTDINISRDQQDHFMVYLFTAL
                     TLIFWIAAYFRVKEKQV"
BASE COUNT          616 a          603 c          573 g          710 t
ORIGIN      
        1 aagcagcagc aaaatacagg taaacggccg gtcgtctgat ccggttctgg atctcgaaca
       61 aaatgatttt ccggaacata gtgcgggaat ttaaaaaagg ctaaatggta tttatatcca
      121 tgcgggtagc gatattgctg aaataaacat cctccaggtt tggcgagccg ggttcaaaac
      181 catttccggg agccgtttcc tgcaaaaccc ggatatgcag tttaccggat ttgagttgcg
      241 tggatatgac gttgtattcc ttttggtatc tgcccagcgc ctccttgccg atcgctttgc
      301 tgaatatttt gccttccatt tcttttaccg ccatttccgg ctcgccggca tataatactt
      361 cgccctggca gatgatggca aaatttgaac agagggtgct gacgtcttcc acaatatgcg
      421 tggagaggat cacaatcgta ttttccccca gctggctcag caggttgtaa aaacggtttc
      481 tttccgcagg atccaggccg gcggtaggct cgtccacaat aatcagttta gggtcgccga
      541 tcagtgcctg ggcgataccg aacctttgtt tcattccgcc cgaataggtt cccagatgtt
      601 ttttccggtc cctggagaga tttacgtttt cgagcaaagc ccccactaat tcccggcgct
      661 caccggcatg atcaattcct tttaaccggg cgatatggtc cagcatccgt tcagcagata
      721 ttttcggata aaccccaaac tcctggggca ggtagcccag catttgtctt acagcggttt
      781 tgtttttcaa tacatccaga tcatccagaa aaatacttcc ctgatcggcc tgctgcaggg
      841 tggcaatcgt gcgcatcagg gatgatttgc cggctccgtt gggtcctaaa agaccaaaca
      901 tgccattgtt taaggtaagc gtaacatcct tcagggcctg taccttgttc gcataggtct
      961 tcgacaggga ttttatagac agtttcattt ctgatccggt tttgaattgc ttaataaatt
     1021 ttgagctttc ccgctgacga gaagttgggc gctgtcgccc aggtcataat gcaggttatc
     1081 gatcattgca ttttccaatt gcagtttgct tttgtggcgg atatccagca ccgcatcctg
     1141 aaaccggttg gtattttgaa tgatcaggcc ggatccgctc ccccggcttt ttccaatgac
     1201 cgcatgtagg gaacggatat ggttattcgc cagcacaaca gtgcttccat aatctgcgct
     1261 gatctgcagg ctgtcctgta taaagccatc gatcaacacc ctccgcatat tccagtcctc
     1321 ccgggcgata ctgtcggtgt acggcccatc gcctgtctgg taggaggcat ctgcgttcag
     1381 tgaagtcagc ttcgggcagg atatgaccag caggtatgga ttgggattac cgtaatactg
     1441 atcttcgaaa ccggcatcga tttgcaaatg attccccttc tgttgaacct ttacgtattc
     1501 cagtgcattt tcattaatac gcacgctgaa tggcccctga actaatctta cattggccac
     1561 cgtggaggaa ttgaggtcca gcacatcgaa attcctgaag ttcagaccga catagttctg
     1621 gtaaggaatt ttataattgc cggaccggta ttcggccttc aataataaat cataaaccac
     1681 cagcgccata agcgtgagca gcaaggccga gaaaatgaat ttattgcttg ttctcatctt
     1741 ctgatttggg ttttaattcg tggttattat ttttaaattg ctcataccat tgcgcaattt
     1801 cttccggacc gatccccagt aaataaatat tggtgaaaaa tactggcagg tcctggcgga
     1861 taaaccgttc tttccggtat gctttcactt tgtcgtaccc gtcggccgca acaaaagacc
     1921 ccagtcctct cttattatat atcaccccca ggttctccag gtactcatag gaacgcatta
     1981 ccgtattcag gtttacctgt agttcaatgg ccaggtctct tacggagggt atcttttgtt
     2041 ctgccggcca tttatccagc aaaatatttt tgctcacgta ggcgacaatt tggaggtata
     2101 tggcttcgtt atctctgaat tccatttttc aatttcttgg ttaaacctgc ttttctttta
     2161 cgcggaagta ggcggctatc caaaagatca gggttaaggc agtaaacagg tataccataa
     2221 aatgatcctg ctgatcccgg ctaatattga tatccgttac ctgaccgttt tctgaaaacc
     2281 ggagatagcc aaaaggggga gccgcttcca ccgcccggcc cagcagggcg ccctgtatca
     2341 ttttattcag gataatcagc agggtgaggg atacaaaaaa aacaaatgcc gtttttatga
     2401 aatgcagccg ctcaaaaaag accgcgccaa aaaaagcgat tgaatgtaga aaggcatagg
     2461 tcagggccat ctccagggga tttcttttaa agatgttgaa ta
//
LOCUS       DASYCU010000135         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_179169, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000135 DASYCU010000000
VERSION     DASYCU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179169"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGM24_02670"
     CDS             <1..263
                     /locus_tag="VGM24_02670"
                     /inference="COORDINATES: protein motif:HMM:NF027664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HEY4154292.1"
                     /translation="IPDSDKVETSWMYNKLVFEGDTFEELAAKMERWYNIKIIFKSDR
                     LLKYRFKGVFSNESVKEALDALRLTARFTYNIHENEIELYKE"
     gene            461..3691
                     /locus_tag="VGM24_02675"
     CDS             461..3691
                     /locus_tag="VGM24_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4154293.1"
                     /translation="MKLSILFVLLSVIEVQAKVHAQGSITLTMQQAEIGRVLNKIEKK
                     TTGEFGFLYNYDLPSLKKKVDIHFEQSSIHDALAQLFSNTDLTYKVLENNLIVVMSSG
                     TEKQDIRVTGRVTGAGGVPLAGVSVQVKDGATGVSTDNDGQYSITAGEKATLKFSYIG
                     YLDQEVAVNGQNVINVVLAVSDRSLDQVVVIGYGSQRKKDLTGAVSVVDAADIANRPI
                     VDPSEALQGKAAGVEVISNSGKPGAGFTIRVRGSSSISAGNDPLYIVDGIPTTDISSF
                     NPADIESFSILKDAASASIYGTRAANGVVVITTKKGVAGKSKINFSTYFGTSSATKKL
                     GVLNAKQYQDYANGQKMSTVITDSMVQANDINWPEEVFRNGSQQNYQFSVAGGNEKSQ
                     HYISLNYLDQKGIIKPANFNRLTGRVNLTTKANDWLTITSSSVISRSHLNDVTDNASV
                     ARGGVVLLALETPPTVPKYNADGTIGVNPLTGWDNPLSAIEGSYAKSLNDRLLSNLGA
                     DVKLFKGLKFESRFGIDYQNNTYTTFLDPFLTVNGRNTSGQQAQTKTNIFTWLSEQTL
                     NYTATFGKSHLTALLGWTAQDEHTETTGISGSFLNPIYRLLPWEDSYIRDSIKGPGTK
                     SIDDWALISYLGRITYDFDGKYLFQANIRSDHSSKFAPNNRNAVFPSFSAGWILSEEN
                     FMKAMPVVSFLKLRAGWGQNGNQEGLGSYDYLSLYPLDPVTGKPVSPGTIAPQTLTWE
                     TSTQTNIGVDASFFNSRLSFTGDFYVKKTNNVLYKIVLPSNAGYNVAPINGATMQNIG
                     EEFQISSKNIVQPDFKWSTDFSISFNQNKVLSLPADISFVNGYGGVYARGNAIALVKG
                     YGLGEFYGYVARGVDPQTGHELYQTNGDSLSDNPSPSARRLLGSAQPTFVYGLTNNLS
                     YRNFDLTVFIQGSQGGKIFNAGRLESEAMVNAANQSSAVLRRWRQKGDITDIPGVSTD
                     ATTNNSLISSRFLENGSYLRFKTITLSYRFDSKLISRLGLASASLYVSGNNLITITRY
                     TGFDPEVNSYGNPGNGTDDRNISLGLDNGAYPQSKMFLVGLNLSLK"
     gene            3728..>4363
                     /locus_tag="VGM24_02680"
     CDS             3728..>4363
                     /locus_tag="VGM24_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154294.1"
                     /translation="MKNNLERKSMMLALLMAGLLSGCTKLLDKQPVTQTIKGTDSTSI
                     SATQAEDLLSGVYAAYKGYANGIEFNILDRVVNGDVISDNCYAGGDNPDNITLDIFKA
                     NALNGNVSRDWADAYAMIAAANSAIKQVASCTDPALSADRKNQILAENMFMRAFTYFD
                     LVRLWGRVPLVLDPPDQTNSETLVKSVIVPQSSVDSVFDAILNDLWFAKDHV"
BASE COUNT         1177 a         1065 c         1089 g         1032 t
ORIGIN      
        1 atatcccgga cagcgataag gtggaaactt cctggatgta caataaactg gtttttgaag
       61 gggacacgtt cgaggaactg gccgcaaaaa tggagcgctg gtacaatata aaaatcattt
      121 ttaaaagcga ccgcctgtta aagtaccggt tcaagggcgt tttttcaaat gaatccgtca
      181 aggaggccct ggacgcgctt cgcttaacgg ccaggttcac gtataacata catgaaaacg
      241 aaatagaact atataaggaa tagaatgaat agcagttaag aagaaaaaga ggcgggaaat
      301 gttagcgcat cccccgccgg atttaaacgg gaacctgata ccaactggga aggcggtgca
      361 gggtttccta ttattaatca ctaaaagtaa atgtatgaaa tttttgcaat cctgcttcag
      421 gcgggatcag ttcaggttgc tctttaaatt gctcgccgtt atgaagttat ccattctgtt
      481 cgtactgctt agtgtaatag aggtgcaggc aaaagtgcat gcacagggct ctattacatt
      541 aaccatgcaa caggccgaaa tcggaagagt attgaataaa attgagaaga agacaaccgg
      601 cgaattcggc tttttgtata attacgatct tccttcttta aagaagaaag tggacatcca
      661 ttttgaacag tcttccattc acgatgcact ggcgcaactt ttttccaata cggacctgac
      721 gtacaaagtg cttgaaaaca acctgatcgt cgtgatgtcg tcgggcacgg aaaaacagga
      781 catccgggtc accggccggg tgaccggagc cggaggagtt cccctggcag gcgtttccgt
      841 gcaggtgaaa gacggggcta ccggggtgtc aacggacaac gacgggcaat attcgatcac
      901 ggcaggggaa aaagccaccc tgaaattttc ttatatcggc tacctggacc aggaagtagc
      961 cgtcaacggg cagaatgtga tcaacgtggt gctggcggtt tccgaccggt cgctcgacca
     1021 ggtggtggtc atcggttacg gatcccagcg caaaaaggat ctgaccggag ccgtatcggt
     1081 cgttgacgcg gcggatattg cgaaccggcc catcgtagac ccgtcggaag cgttgcaggg
     1141 gaaagccgca ggcgtggaag ttatttccaa ttccggaaag cccggtgccg gcttcaccat
     1201 ccgggtgaga ggttcttcct ccatcagtgc cggtaacgat cccttgtata ttgtggatgg
     1261 aattccgacc accgatattt cctcctttaa ccccgcagac atagagtcct tctccatttt
     1321 aaaggatgct gcctccgcct ccatatatgg aacgcgtgcg gcaaacggag tggtcgtgat
     1381 caccactaaa aaaggagttg cgggaaaatc taaaattaat ttcagcacct acttcggaac
     1441 cagttcggca accaaaaaac tgggtgtatt aaacgcaaaa caataccagg actacgccaa
     1501 cgggcagaag atgtccaccg tgatcaccga ttccatggtg caggccaatg atatcaactg
     1561 gccggaagag gttttccgga acggaagcca gcagaattat caattctccg ttgcaggagg
     1621 gaacgaaaaa tcccagcact atatctccct gaattacctg gaccagaaag ggatcattaa
     1681 accggcaaac ttcaaccgct tgacgggccg ggtgaacctt accacaaaag ccaacgactg
     1741 gctgaccatc acgtccagct ccgtgatcag caggtctcat ctcaacgatg ttaccgacaa
     1801 tgcttctgtt gcccgcgggg gcgtggtatt gctggcgttg gaaacacctc cgaccgttcc
     1861 gaaatataac gcggacggaa cgattggcgt taacccgctg accggatggg ataatccgct
     1921 gagcgccatc gaaggctctt acgcgaaaag cttaaatgac cggctgttat cgaacctggg
     1981 tgcggacgta aaactgttta agggattgaa attcgaatcc cgcttcggaa tcgattacca
     2041 gaataatacc tacaccactt tcctggatcc ctttcttacc gtgaacggac gaaatacttc
     2101 cgggcagcaa gcgcagacca aaaccaatat ttttacctgg ttaagcgaac agaccctgaa
     2161 ttatacggcc acctttggca agagccacct tactgcgctc ctgggttgga cggctcagga
     2221 cgagcatacc gaaacaaccg gaatctccgg gtcctttctg aatccgattt accggctcct
     2281 gccctgggag gacagctata tccgggactc gatcaaggga ccgggaacca aatcaataga
     2341 tgactgggca ctcatttcat acctcggacg gatcacgtat gatttcgacg gaaaatattt
     2401 attccaggcg aatatccgtt ccgatcattc ctccaaattc gctccgaata accggaatgc
     2461 cgtatttcct tctttctccg caggatggat actatccgaa gagaatttca tgaaggccat
     2521 gccggtggtc agtttcctga aattgcgcgc cggctgggga caaaatggta atcaggaagg
     2581 tctcggcagt tacgattatc tttcccttta tccgttggat cccgtaaccg gcaagcccgt
     2641 ttccccggga acgattgccc cacagaccct gacctgggag accagtaccc agaccaatat
     2701 cggagtggat gcgagctttt ttaattccag attatcgttc accggcgatt tttatgtaaa
     2761 aaagactaat aacgttttgt ataagattgt actgccttcc aatgcaggat acaatgttgc
     2821 cccgatcaac ggcgccacca tgcagaacat cggggaagaa ttccagatct ccagtaagaa
     2881 catcgtgcaa cccgatttta agtggagcac cgatttcagc atttccttta accagaacaa
     2941 ggtcctcagc ctcccggcag acatctcctt tgtaaacggt tacggaggcg tctacgccag
     3001 gggaaatgcc atcgccctgg ttaaaggata tggattggga gaattttacg gatatgtcgc
     3061 caggggcgtg gatccgcaaa ccggccatga attgtatcag accaacggcg attccctgtc
     3121 cgataatcct tcgccctctg cgcgccggtt attgggaagt gcccagccga cctttgttta
     3181 tggcctgacc aacaatcttt cgtacaggaa cttcgatctt accgtattta tccagggatc
     3241 ccagggaggg aaaatattca acgccgggcg gctggaatca gaagcgatgg tcaatgcggc
     3301 caatcaaagt tcagcggtgc tgagaagatg gaggcagaaa ggagacatta cggatattcc
     3361 gggcgttagt acggatgcga ccacaaacaa ttccctgatc tcctcccgct ttctggaaaa
     3421 cggatcctac ctgaggttta aaaccatcac cctgagctat cggtttgaca gcaagctgat
     3481 ttccaggttg ggactagcct ccgcttccct gtatgtttcc gggaacaacc tgatcacgat
     3541 taccaggtat accggatttg atccggaagt gaattcctat ggaaatcccg gcaacggcac
     3601 cgatgacagg aatatttctt tgggcctgga taatggcgct tatcctcaat ccaaaatgtt
     3661 tttggtgggt ttaaacttga gcttgaaata gggttttaaa aaaaggtctg caaaaaaaaa
     3721 attcatcatg aaaaataatt tagaacgtaa atcgatgatg ctggccctgc tgatggcagg
     3781 gttactgtcc ggatgtacaa agctgctgga taaacagccg gttacgcaaa cgataaaggg
     3841 aacggattcc acttccattt cggctaccca ggcggaagac cttctttccg gagtatatgc
     3901 cgcttacaaa ggatatgcga atgggattga attcaatatc ctggaccggg tagtgaacgg
     3961 agatgtcatc tccgataatt gttatgccgg cggagataac ccggataata ttacactgga
     4021 tattttcaaa gcaaatgcat tgaacggaaa tgtttcccgg gactgggccg atgcctatgc
     4081 catgattgcc gctgcgaact ccgctatcaa gcaggttgcc agctgtaccg atccggcttt
     4141 gtctgccgac cggaaaaacc agatcctggc agagaatatg ttcatgcgcg ccttcactta
     4201 ttttgacctg gtccggctct ggggaagagt tcccctcgtg ctggatccac cggatcaaac
     4261 caattccgag acgctggtaa aatcggtgat cgtgccgcaa tcttccgttg attcggtttt
     4321 tgacgcgatt ctgaatgatc tctggtttgc taaagatcac gta
//
LOCUS       DASYCU010000136         4838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4528349, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000136 DASYCU010000000
VERSION     DASYCU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528349"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGM24_02685"
     CDS             complement(<1..332)
                     /locus_tag="VGM24_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY4154295.1"
                     /translation="MQLVTLIAGKTGSTETARELTQDIFLAIYQQKKELHHIEDFKAY
                     LFGLAKNKVYNYYRHELVKKKYRTLVLMNDYKILSSEGIEILENKEQLQLINNQIEKL
                     PPKCREVF"
     gene            603..1775
                     /locus_tag="VGM24_02690"
     CDS             603..1775
                     /locus_tag="VGM24_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY4154296.1"
                     /translation="MVKQRLLYLLNKQEDKRLTDTEHQELEEWYALLNKDATDLAEQY
                     RLNPEWSEEMLREFRQNRLNQAATIRKIRSPFGGVRVAAILVGICLTAGIYFFFSRGQ
                     QKEMAHINKPPEQIRKDPEPGGNKAILTLANGMQIVLDSASNGTLAIQGNVKVTKLAN
                     GRLAYSKMDGPAHEELFNTMSTPRGGQYELVLPDGTKAWLNSASAIRYPTTFTGRQRT
                     VEISGEAYFEVAPSTSAVTGEKIPFKVSMYDGEEIEVLGTHFNVNAYPEEGAVKTTLL
                     EGSVRLTKLNKSVLIVPGQQAILTTKGYHITTPDLEKVIAWKAGFFDFDDMDLATIMR
                     QISRWYDVDVVFHGKTNAFTFGGRISRNLPLSNVLKMFEENGVAFRMEGKVLHVEL"
     gene            2011..>4838
                     /locus_tag="VGM24_02695"
     CDS             2011..>4838
                     /locus_tag="VGM24_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154297.1"
                     /translation="MRITAIILISTCLQVAARSDAQTISFSLKDVPVQKIFKEVSRQT
                     GISIVYSETLFKSFDRVTISVKDASISEVLDKCLKNLPFYYSLQGKTVVIKRKGSPTA
                     PDQPVENLLPPVDLKGRVINEKGEALAGATVQVKGSRQFIVTGADGYFSMKVPAEEKI
                     VLIFSYVGMETQEQAAKEGVELLIRLKPVNAALSDFVVVGYGTSRKRDITGSVASVAM
                     NEQEKTPVIGTEQLLEGRVSGVQITQNQSQPGAVFSVRIRGTNSINSSSSPLFVVDGY
                     AGGNGGDLNPSDILSIDVLKDASATAIYGSRGANGVIMITTRRGSLNAPKVSIDAYAG
                     LQQIGKEYKMMDARQYAVFANTVHQENNDINNTSDPLPFTESQINALGKGTDWQDAIF
                     RKASISNYSIAFNGGNADSRHYLSFNFFDQKGIITGSDYKRGIVRFNLDQNIGTKLKV
                     GVSSQIGYSYQNMTTVNTSGGADQPSVLWDAVRFNPMLPVRDSTGAYTYVNGPAGVGA
                     PIGNPVAYINEAKDGFYNLNVFVNAFGEYEIWKGLKFRTSFGANYATSGEEAFVPTDI
                     FASSGIGRASQASARNYNWLNENTLTYDAVFNKIHAINLTGGFTLQNWYTKTFSAGIT
                     NLSTNNLGPYNFGIGIPSAPTSYYGENALVSYFGRANYRLMDKYLFTFTMRADGSSRF
                     GANDKWGYFPSGAFAWHLSQENFVKNINAVSDLKLRMSYGITGNQEIGSYNSLSQFVS
                     NQYSLGVTPSLVVGVSAANIANPDLKWESTASSDLGLDLGLWNNRLYITTDFYYKKTS
                     NLLLLVNIPITSGYSSILQNAGAVSNKGFEFSATSLNIDQKKVKWTTTFNFSTNVNKV
                     LNLGSNKQIYVGQLSGSVFNGGAPSSAILEPGKPIGSFYGYVFDGIWQSQADIIKSGT
                     KQAVNPGDPIYKDLNNDSSLSGDDRTILG"
BASE COUNT         1324 a         1042 c         1191 g         1281 t
ORIGIN      
        1 ttaaatactt ccctgcattt tggtggcagc ttctcgatct gattattaat tagctgcaac
       61 tgctctttgt tttccagaat ttcaatgcct tcggagctaa gaattttata gtcattcatc
      121 aataccagag tacggtattt cttttttacc aattcatgac gataatagtt atatacttta
      181 ttttttgcca ggccgaacag gtatgcttta aaatcttcga tgtgatgcag ctcctttttc
      241 tgctggtaaa tagcgagaaa aatatcctgt gtcagttccc tggctgtttc tgtggaaccg
      301 gtcttgccgg cgatcagggt caccagttgc atgatatgtc ttttgtataa tacatcgaat
      361 gcgatttcat caccctgttg ccacaggctg atgagggaaa gatcgttatc gcacggcagg
      421 tttttgatgg caactattga taatcaaatc taccaaaaat taatatacaa ccggttcggt
      481 tttgtctctt ttccgccggg tataaaaaaa taatgatgcc ggataacgga atggcaggtt
      541 tcaggttact aataacacag gatatcttca ggatcaggga aaattccaaa ccgttctaat
      601 ttatggttaa acagcgtttg ctttatttac tgaataaaca ggaagacaag cgtctcacag
      661 acacggaaca ccaggaattg gaagaatggt atgctttgct gaacaaagac gcgaccgatt
      721 tagcggaaca gtaccggctt aacccggaat ggagtgaaga aatgctccgg gaattccggc
      781 agaaccggct taatcaggcg gcaaccattc gcaaaataag gtcacctttc gggggggtcc
      841 gggtagccgc gattctggtt ggcatctgtt tgacagccgg catttatttc tttttttcca
      901 gggggcaaca aaaggagatg gcgcatatca ataaacctcc ggaacaaatt agaaaagatc
      961 cggaaccggg cggcaataaa gccatactga cgctggcgaa tgggatgcag atcgttttgg
     1021 atagcgcttc gaatggcacc ctggctattc agggtaatgt taaagtgacc aagcttgcca
     1081 atggccggtt ggcgtatagc aaaatggatg gacctgccca tgaagaacta tttaatacga
     1141 tgagtacgcc caggggtggc caatacgaac tggtattacc ggatggtact aaggcgtggt
     1201 taaactcagc ctcggccatc cggtatccga ccacttttac cggaaggcaa agaacggtag
     1261 agattagtgg agaggcctat tttgaagtag ctccgtccac ttcagccgtt accggagaaa
     1321 aaataccttt taaggtttcg atgtatgatg gggaagaaat agaagtgctt ggtacgcatt
     1381 tcaacgtgaa tgcttacccg gaagaaggtg ctgttaaaac gaccctgctg gaaggttcag
     1441 tgcggctgac caagctgaac aaatccgtcc tgattgtgcc tggccagcag gcgatcctta
     1501 caacgaaggg ctatcatata acgacgccgg atcttgaaaa agtaatcgcc tggaaagcgg
     1561 gcttttttga tttcgatgac atggatctcg ccaccatcat gcgacagatt agccgctggt
     1621 atgatgtaga tgtcgttttt cacggtaaga cgaatgcctt cacatttggc ggccgaatca
     1681 gcagaaatct gcccctgtct aatgtgttga agatgtttga agaaaacggc gttgctttcc
     1741 ggatggaagg aaaggtgctg catgtggagc tttagccgga tgacggaaat ttcatgaatt
     1801 cggattccga ttaacgtaaa caaaaaaccg gaagtgttta gaccacctcc ggtagaacgt
     1861 tgagctaact ttttaataca attgttcgaa ctgcttattc attaacccaa acattgcaaa
     1921 gttatgtatt ttagattgct tagccccggt ttcactccgg gagttccctg tatcccgcga
     1981 catgcgggat ttaccaaaac cctgcggatc atgaggataa ccgccatcat cttaatcagc
     2041 acctgccttc aggtggccgc ccgctcggat gcgcagacca ttagcttttc actgaaagat
     2101 gttccggtgc aaaaaatatt caaggaagta tcccgtcaaa cgggtatttc cattgtttac
     2161 agtgaaaccc tgtttaaaag ttttgaccgg gttaccatta gtgttaagga tgccagcatt
     2221 tcagaagtgc tggataaatg tctgaagaac ctgcctttct attatagtct gcaagggaaa
     2281 accgtggtca tcaagaggaa gggttcaccc acagcaccgg atcagcctgt tgaaaatctt
     2341 ttaccgcctg ttgatcttaa gggtcgggtg atcaatgaaa agggggaggc gctggcgggt
     2401 gccacggttc aggtaaaggg atccaggcaa tttattgtaa ccggtgccga tggttatttt
     2461 tccatgaaag ttccggccga agaaaagatt gtcctgatct tttcttatgt aggaatggaa
     2521 acccaggagc aggctgcaaa agagggtgtc gaattgctga ttagattaaa accggtgaat
     2581 gccgccttgt cggattttgt agttgttgga tacggaacct cgagaaaaag ggatatcacc
     2641 ggctctgttg catcggtagc gatgaacgag caggaaaaaa caccggtcat tggaactgaa
     2701 cagctgctgg aaggtcgggt tagtggggtg cagattacgc aaaaccaatc ccagccgggc
     2761 gctgtcttct ccgtaaggat acggggaacc aattccatta attcgagcag cagtccgcta
     2821 tttgtcgtgg atggttatgc tggtggcaac gggggagatc ttaatcccag cgacatttta
     2881 tccattgatg ttttgaaaga tgcttcagca acggccattt acggaagtcg cggagccaat
     2941 ggcgtgatta tgatcactac caggagagga agccttaatg cgccgaaagt gtccatagat
     3001 gcttatgcag gtctgcagca aattggaaag gaatacaaga tgatggatgc cagacaatat
     3061 gctgttttcg caaatacggt acatcaggag aacaatgata taaacaacac ttccgatccg
     3121 cttcccttca cagagtcgca gatcaatgcc ctgggtaaag ggaccgactg gcaggatgcc
     3181 atattcagaa aagccagcat atccaattac tccatcgcct ttaacggtgg taatgcggat
     3241 tccagacatt atctgagttt taattttttt gatcagaagg gcatcatcac cggatctgat
     3301 tacaagcggg gaattgtccg gtttaacctg gatcagaata tcggtaccaa attaaaagtt
     3361 ggcgtatcat cccagattgg atacagttat cagaatatga ctacggtgaa cacctccggg
     3421 ggagctgatc aacccagtgt tttatgggat gctgtacggt ttaatcccat gcttccggtc
     3481 agggacagca caggagcgta tacttatgta aacggacctg cgggcgttgg tgcaccgata
     3541 ggtaatcctg tggcttatat taacgaagcc aaggacggct tttataattt gaacgtattt
     3601 gtgaatgcat tcggagagta tgagatttgg aaaggattga aattcagaac cagctttgga
     3661 gccaattatg caaccagcgg cgaagaggcc tttgtgccca cggatatatt tgcatcatcg
     3721 ggtattggcc gcgcgtccca ggcttccgcc agaaattaca attggctcaa tgaaaacacg
     3781 ctgacctatg atgccgtatt taataagatc catgcgatca atctgacagg gggatttacg
     3841 cttcaaaact ggtatacgaa aacgttctct gccggtatta ccaatctgtc taccaataac
     3901 ctggggccat ataatttcgg tatcggtatt ccctctgccc ccacttccta ttacggcgaa
     3961 aatgcgctgg tttcctattt cggcagagcc aattaccggt tgatggataa atacctgttc
     4021 acctttacca tgcgtgcaga tggttcttcc cgtttcggag ccaatgacaa gtgggggtat
     4081 tttccctccg gagcatttgc ctggcatctg tcgcaggaaa acttcgttaa aaatataaat
     4141 gccgtcagtg acctgaaatt acggatgagt tatggcatta ccggtaacca ggaaattggt
     4201 tcctacaatt cgctgagtca gtttgtttcc aatcaatact ctctgggggt tacgccctct
     4261 ctggtcgttg gggtttcagc cgccaatatc gcaaatcctg atttgaaatg ggaatccacc
     4321 gcttcatccg atctgggtct ggacctgggg ttgtggaata accggctata tatcacaacg
     4381 gatttttatt ataaaaagac ctccaacctg cttttgctgg tgaatatccc cattacctcc
     4441 ggatacagca gcatccttca gaatgccgga gcggtatcga acaaggggtt tgagttttca
     4501 gccacgtccc tgaacattga tcaaaagaaa gtgaaatgga ccactacgtt caatttttca
     4561 acgaatgtca acaaagtatt gaatctgggg agcaataaac aaatttacgt cgggcaactt
     4621 agcggttctg tgtttaacgg aggagcgcct tcctccgcca tcctggaacc gggaaagccg
     4681 atcggatcat tttacggcta tgtgtttgac ggcatctggc aatcacaggc agatattatc
     4741 aagagtggca caaaacaggc agttaatccc ggcgacccga tttataagga tctgaataat
     4801 gacagttcat tgagcggcga cgaccggacc atactggg
//
LOCUS       DASYCU010000137         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2351980, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000137 DASYCU010000000
VERSION     DASYCU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351980"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VGM24_02700"
     CDS             complement(<1..1443)
                     /locus_tag="VGM24_02700"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEY4154298.1"
                     /translation="MKDKSFVIGVDFGTDSVRAIIVDAASGAEAAASLFYYPRWKAGQ
                     YCNPSINQFRQHPKDYLEGLEYTIRDCVRKAGPEISSRIGAIGVDTTGSSPVAVDKTG
                     TPLALLPEFAENPNAMFVLWKDHSSVKEAAQINAHAKKFSTDYLQYVGGIYSSEWFWA
                     KLLHVLRQDDAVRKACFTWVEHCDWIPFLLTGGTDVNRMKRGVCSAGHKALWSEAFGG
                     FPPDEFFSSLDPLLEGHAARLPSGTFTSDQPAGTLSPYWANQLGLTGDVVVAVGAFDA
                     HMGAVGGQIEPYYLSKVMGTSTCDMLVAPAADMKDKLVKGICGQVNGSVIPGMVGMEA
                     GQSAFGDAYAWFKNLLAWPLQLASEKAIRSNDSGLAAIIEETLLEMLPELNLQAAQIP
                     VTEKSEIAVDWFNGRRTPDANQELKGAISALNLASNAPAVFRAVAESTCFGARAIVER
                     FNSEGIPVKGLIGLGGVARKSPFIMQLMADI"
     gene            complement(1525..2232)
                     /locus_tag="VGM24_02705"
     CDS             complement(1525..2232)
                     /locus_tag="VGM24_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4154299.1"
                     /translation="MSKYAGQTHVLAAVDCIIFGFDGEDLKVLLFKRVIEPEKNKWSL
                     IGGFLTKSESLEQAAVRVLKKFTGIEGLYLGQLRTFSDPNRDPVERTISTAFTALVDI
                     HKYEKQISKEYHAEWFLIPKRPSLIFDHDDMLRMAQKELRYQAASHPILFELLPEKFT
                     IPQLQNLYEGVYQTNLDNRNFSRKVLSTELLIKLKEKDKSNSKKGAFYYKLNKKKYKA
                     SFRAFMNFIPNPDKFVL"
     gene            2278..>4027
                     /locus_tag="VGM24_02710"
     CDS             2278..>4027
                     /locus_tag="VGM24_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HEY4154300.1"
                     /translation="MDNRGSVRRNEGSGKSGSRIFPLSIAERLFIFAAIMTTKPFLTN
                     EAGIGGQYKNWFLDYASYVILERAVPALQDGLKPVQRRILHAMKEMDDGRFNKVANII
                     GQSMQYHPHGDASIGEAIVNLGQKDLLIETQGNWGDVRTGDDAAAARYIEARLSKFAL
                     EVAFNPKTTEWQLSYDGRKNEPITLPMKFPLLLAQGAEGIAVGLATKILPHNFCEICQ
                     AAVKYLRGKKFELMPDFQTGGMIDTANYNEGKRGGRLRVRAHIEEEDKKTLNIKDVPY
                     GITTSQLIDSIIKANDQGKIRIRKVTDNTAAEVEIQVDLAQGISPDITIDALYAFTDC
                     EISISPNACVINKQKPEFPTVQELLKISVDDTKELLRRELEIKLAELQDKWHYTSLEK
                     IFFEEKIYKELEKKHETWEKVLAAIAKAFEPFRKQLKRNITREDVVKLTEKPVRRIYK
                     LDIEELNNQIKALEAEIKQVKFDLSNLVDFAVNYYDNLLKKYGKGRERKTEIRPFEAI
                     EAKQVAIANTKIYVNRSEGFIGTGLRKDEFVTECSDLDDIIAFTRSGKMKIVKVAEKS
                     FIGKDIIHVGVFQKNDE"
BASE COUNT         1040 a          999 c         1082 g          906 t
ORIGIN      
        1 aatgtccgcc atcagctgca tgatgaaggg cgattttctg gcgacccctc cgagcccgat
       61 caatccttta acagggatcc cttcgctgtt aaatctttcc acgatcgcgc gggcgccgaa
      121 acaagtggat tcggcgacgg cgcggaaaac ggccggcgca ttgctggcca ggttcaatgc
      181 ggagatggcg ccctttaact cctggttggc atccggcgtc cggcggccat taaaccagtc
      241 aacggcgatc tccgattttt cggttacggg aatctgtgca gcctggagat tcaactccgg
      301 cagcatttcc agcagggtct cttcgatgat ggccgctaag ccggagtcgt ttgaccggat
      361 cgctttttca gaagccagct gcaggggcca ggccagcagg ttcttaaacc aggcataggc
      421 atcgccgaaa gcggattgcc cggcttccat cccgaccatg cccgggatca ccgaaccgtt
      481 cacctgtccg cagatccctt tcaccagttt atctttcata tcggccgcag gcgccaccag
      541 catatcgcag gtggaggtgc ccatcacctt actgaggtaa tagggttcga tctgcccgcc
      601 gacggctccc atatgggcat cgaacgcgcc caccgccacc accacgtcgc cggtcaggcc
      661 aagctggttt gcccagtatg ggctcagggt tccggcaggc tgatcggagg taaaggttcc
      721 ggaaggcagg cgtgcggcat ggccctccag caggggatcg agcgaggaaa aaaattcatc
      781 cggcggaaaa cctccgaatg cttccgacca taatgcttta tgcccggcac tgcaaacgcc
      841 ccgcttcatc cggttcacat cggttcctcc cgttaaaaga aaagggatcc agtcacagtg
      901 ttcaacccag gtaaagcagg ctttccgcac ggcatcatct tgccgcagca cgtgtaataa
      961 tttagcccag aaccattcgg aagaatagat gccgccgaca tattgcaggt aatccgtgga
     1021 gaactttttt gcgtgcgcgt tgatctgagc cgcttctttt accgagctgt ggtctttcca
     1081 caacacgaac atggcattgg gattttcggc aaattcggga agcaaggcca ggggagtacc
     1141 ggttttgtcg acggccaccg gagaagaacc ggtggtatcc acgccaatgg ccccgatgcg
     1201 cgaagagatc tccggacccg ccttgcgcac gcaatcccgg atcgtgtatt ccaggccttc
     1261 caggtaatcc ttcggatgct ggcggaactg gttgatggag ggattgcagt actgtccggc
     1321 cttccagcgg ggataataaa aaagagatgc ggcggcttct gctccggatg cggcgtccac
     1381 gataatcgcc cggaccgaat cggtcccgaa atcaaccccg atgacaaatg atttatcttt
     1441 catatcagcc tccgtcgccg gaattcatgt caaattaaca cttaattgat tgtcagcaaa
     1501 aaaaaacagg ccatgcccgg tgccctataa aacgaattta tccgggttgg gaataaaatt
     1561 catgaatgcc cggaaactgg ctttatattt tttcttgttc agtttataat agaaagcgcc
     1621 ttttttggaa ttggatttat ctttttcctt cagcttgatc aggagttcgg tggataggac
     1681 cttgcggctg aaattccggt tgtcgaggtt ggtctggtag actccttcgt agagattctg
     1741 caattgggga atggtgaatt tttccggaag cagttcaaac aggatcgggt gggaggccgc
     1801 ctggtaccgg agttccttct gggccatcct cagcatatcg tcatggtcaa agatcaggga
     1861 cggccgtttg gggatgagga accattccgc atggtattcc ttgctgatct gtttttcgta
     1921 tttgtgaata tcgaccagcg ccgtaaaagc ggtggaaatg gttctctcca cggggtcgcg
     1981 gttcggatca ctgaaggtgc ggagctgacc caggtagagg ccttcgatgc cggtgaattt
     2041 tttcagaacg cgtacggcgg cctgttccag gctttcggat ttagtaagaa atccaccaat
     2101 aaggctccat ttgttttttt ccggttcgat cacccgttta aataaaagga ctttaagatc
     2161 ctccccgtca aatccgaata taatgcagtc aactgctgca aggacatgtg tttgtccggc
     2221 atatttactc atataagaaa gcagttattc tgaggtgaat ataggaattg ttaatcaatg
     2281 gacaatcggg gaagcgtccg gcggaacgaa ggatccggga aatccggatc gcgaattttc
     2341 ccattgtcta ttgcggaacg cttatttatc tttgcagcca tcatgaccac caaaccgttc
     2401 ctgacgaatg aagccggcat tggcggccag tataaaaact ggttcctgga ttatgcgtct
     2461 tatgtgattc tggaacgggc cgtgcctgcc ttgcaggacg gattgaagcc ggttcagcgg
     2521 cgcatacttc atgccatgaa agaaatggat gatggccgct tcaataaagt agccaatatc
     2581 atcgggcagt ccatgcagta tcatccgcac ggggacgcct ccatcgggga agcgattgtg
     2641 aacctgggac agaaggatct gctgattgaa acccagggaa actgggggga tgtccgcacc
     2701 ggggacgatg ccgcggcggc aagatatatc gaagcgcgtc tcagcaagtt tgcgctggaa
     2761 gtagctttca acccgaagac cacggaatgg cagctcagct atgacggaag aaagaatgag
     2821 cccatcaccc tgcccatgaa gtttccgctc ctgctggccc agggcgcaga agggattgcg
     2881 gtgggactgg ctacgaaaat tctaccccat aatttctgcg agatctgtca ggccgcggta
     2941 aaatatttgc gcggcaaaaa attcgaactg atgccggatt ttcagaccgg tggaatgatc
     3001 gataccgcca attacaacga aggaaaaaga ggcggcaggc tgcgggtccg ggcccatatt
     3061 gaagaagagg ataaaaaaac cctgaacata aaagatgttc catacgggat cacgacaagc
     3121 cagctgatcg attccatcat aaaagcaaac gatcagggaa agatcaggat ccggaaagta
     3181 accgacaata ccgccgccga agtggagatc caggtggatc ttgcccaggg catctctccg
     3241 gatatcacca tcgatgcctt atatgctttt acggattgtg aaatcagcat ctcaccgaat
     3301 gcctgcgtga taaacaaaca aaagccggaa ttccccaccg tccaggagct gttgaagatt
     3361 tcggttgacg ataccaagga actgctccgg cgcgaactgg aaatcaaact cgccgaactg
     3421 caggataaat ggcattacac ctcgctggaa aaaatcttct tcgaggagaa gatttacaag
     3481 gaactggaaa agaaacacga aacctgggag aaagtgctgg cggcgatcgc caaggcattc
     3541 gaacctttcc gcaagcagct gaaacggaat atcacccgcg aggatgtggt caaactaacg
     3601 gagaagccgg tccggagaat ttacaaactg gacatagagg aattgaataa ccagatcaaa
     3661 gccctcgagg cagagatcaa acaggttaaa tttgatctca gcaacctggt ggatttcgcc
     3721 gtgaattatt atgacaacct gctgaaaaaa tatggaaagg gccgtgaacg gaaaacggag
     3781 atccggccgt ttgaagccat cgaggcaaag caggtggcca tcgcgaatac aaaaatttat
     3841 gtgaaccgtt cggaagggtt tatcggcacc gggctcagga aagatgagtt tgtaaccgaa
     3901 tgctccgacc tggatgatat cattgccttt acccggagcg gcaaaatgaa gatcgttaag
     3961 gttgcggaaa aatcatttat cggcaaggac atcattcatg tgggtgtttt tcagaagaat
     4021 gatgaac
//
LOCUS       DASYCU010000138         7964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5109574, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000138 DASYCU010000000
VERSION     DASYCU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5109574"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGM24_02715"
     CDS             complement(<1..525)
                     /locus_tag="VGM24_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4154301.1"
                     /translation="MKKVLITAKVHEYLIERLSARGYQIIHLPQITYEELETAVADIE
                     GLIVTTRLKVDRNILERANLLKWIGRLGSGMELIDVPFAESKGIQCVSSPEGNRNAVA
                     EHVLGMLLSLMNKMPKAFQEVKEGKWIRDANRGTELTGKTVGIIGFGNTGSAFAKLLA
                     SFEVTILAYDKYKYG"
     gene            complement(598..1368)
                     /gene="rsmA"
                     /locus_tag="VGM24_02720"
     CDS             complement(598..1368)
                     /gene="rsmA"
                     /locus_tag="VGM24_02720"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY4154302.1"
                     /translation="MTHLPRSMNLKKSLGQHFLKDESVCRQIVDALGEHSFSQLLEIG
                     PGAGALTKYLIGIKEIHFRAVEIDGEKAAFLKKTYPALIPDLIEKDFLTMDMPFGGRF
                     TVIGNFPYNISTQILFKILDWRESVERVIGMFQKEVAQRIAAAEGNKTYGVISVLIQA
                     FYQVEYLFEVSEQAFTPAPKVKSAVIRLTPLTEPLPAKTEKLFFLLVKTAFNQRRKML
                     RNAVKGLFSEDTLKDPVFNRRAEQLTIREFADLTWKMR"
     gene            1434..2522
                     /gene="pdxA"
                     /locus_tag="VGM24_02725"
     CDS             1434..2522
                     /gene="pdxA"
                     /locus_tag="VGM24_02725"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY4154303.1"
                     /translation="MSIPLQRPVIGISIGDTNGIGTELVIKTFSDNRLLELCTPVLFA
                     SNKLINFYRKSIPESSFNYQNVKDLSRIYPKQVNILNCWEEEISITPGQLNETGGKYA
                     VKSLVAAVEALKDKKIQGLVTAPIHKKNIQNSEFSYTGHTPYLLQAFGMKDVLMLMSS
                     ENFRVGLVTEHVPVSEIAKHITREAILSKLNIMKDSLIRDFGIDKPRIGVLGLNPHAG
                     DEGLIGKEEEEIIKPAIKDAKHNMLVFGPYSADAFFAHNQQQKFDAVLAMYHDQGLIP
                     FKSLAFGEGINYTAGLPAVRTSPDHGTAFDIAGKNKADHESFMAAVFGCLAILDKRRD
                     FNENHKNPLKKMGSVVLAGAEDEKIDEG"
     gene            2759..3442
                     /locus_tag="VGM24_02730"
     CDS             2759..3442
                     /locus_tag="VGM24_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4154304.1"
                     /translation="MKRIIRFALPLLLAITGGLFAHAIPSTATDTANNSISLTLHGSL
                     AVFNAAYGRNYFELNWNTVAGNFDHFEIERSLDGQTFEKLGDVKAGEMGSTGRYMFRD
                     HFKAVTARNNDFFYRLKQFDPNGNSSYSKVLIARMYNTRSLASLSVTPDPVANDILVN
                     VQLNQNAYVMMKILDADGNLVLKEGRKADTGSSTYSLNDTHNLQKGMYTLEVTVNSRE
                     KLVMKLMKG"
     gene            complement(3545..3847)
                     /locus_tag="VGM24_02735"
     CDS             complement(3545..3847)
                     /locus_tag="VGM24_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY4154305.1"
                     /translation="MNLFKDLDPVLHSQLRLAIVSLLMSHQQAEFTAIREQTNATAGN
                     LSVQINKLREAGYIEVVKEFKDNYPRTLCRITLAGKKAFDSYVKSLQSYLSVNGNS"
     gene            complement(3919..4545)
                     /locus_tag="VGM24_02740"
     CDS             complement(3919..4545)
                     /locus_tag="VGM24_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154306.1"
                     /translation="MKSILFSLFLFAAIGSMAQSDRYTEAMKKNLAQFGTAKTTADYQ
                     SVANTFERIGDAEKTQWLPYYYAGLALSTAGWSDPKLDKDANSTRINALCDKAEAIEK
                     NAEIYALRNMSATQQMMVDPQTRWQTYGQQASAALQSGLKLDPDNPRLYYLQGESVMN
                     TPEAFGGGKAKAKPIFEKAIELFKKQQPKPLYPDWGQARAEEELAKCQ"
     gene            complement(4570..6855)
                     /locus_tag="VGM24_02745"
     CDS             complement(4570..6855)
                     /locus_tag="VGM24_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4154307.1"
                     /translation="MFSCFKTQHYKNQRFKNTGLLFLILFCLQTGFSQTIVSGKIKDG
                     RGHPVPGASITIKNSFDGATSDSSGNYRFSASDTGNQQITVTSIGYRPLVQPILLKGE
                     AVYLDLQLKEEPNEMKAVTITAGSFSAGDSKRGAVLSSLDVATTAGSNADITAALKTL
                     PGAQQVGEQEGLFVRGGTSQETKQFIDGSLVNNPYYTSVPDISTRGRFSPFLFKGTVF
                     STGGYSALYGQALSSVVLLESIDLPEKSEVDASISPIVVGVGTQQLAKNKKSSWGISY
                     NYVNVGVYFGIVKQTPDYFRLPQFHSGDANFRVKTKNGGMIKYYTTFNYNELGLRRPD
                     VDSSYLKNAFDLGNHNWYNNLSWREYLRNGWKMNLSGSYSTNLDHINQQVEGPDNRPH
                     YYTGSTFWMSNKNFVLKNREDLAQTKAILEKKLGGLSAFRFGAEYWYNNNPFVYQDTS
                     RRLKDNMLAGFAETDIYLTNELAVKAGLRYEHSSLIRKSDLAPRISLAYKTGKDAQIS
                     AAYGIFYQKAENQQLFYTSDLGFTQATHYIINFQKMTRDQIFRVEAYYKKYKDLIKTV
                     PVNYYYFNYNNTGRADARGIEFFWRDKKTFKNFDYWISYSYLDTKRDYLNYPGLITPN
                     FAAKNTVSLVMKRFFTDWKTGFNLTYTYASGRPYYDFMINNSGKYYTADQGYTKDYHS
                     LNFSMEYVPSIGKTNPKTFVVLFASMSNVLGYNAVYGYNYSFNGQQKQAIEPPANRFY
                     FIGCFLSWGVDRTQDAINNNL"
     gene            7049..7561
                     /locus_tag="VGM24_02750"
     CDS             7049..7561
                     /locus_tag="VGM24_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154308.1"
                     /translation="MRWSNTENEKHTIERFDLLDGHSLEMTLRYNPIQHSGRLERNNN
                     HLLFFIEQTGLWGNKFLFENEYGVEIGRISFDNLQNLDGSIHIDRKKLSFKIPLNRSG
                     DVFFVPNKSLKQNFHCSIDAGSILSGPDKKVRTEQSEKYACLILGLFWYLFPESIPEA
                     NKSGVSIALH"
     gene            complement(7652..>7964)
                     /locus_tag="VGM24_02755"
     CDS             complement(7652..>7964)
                     /locus_tag="VGM24_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4154309.1"
                     /translation="DLDSKFRYSDVIVVKVKNEEQRLMLYPNPVARELRITIPDSWQN
                     KTVNYKLFNSNGVLMRQLLSVNAGQTETMQVADLAAGVYIIQTATGKETAVQKFIKTN
                     S"
BASE COUNT         2081 a         1932 c         1781 g         2170 t
ORIGIN      
        1 accgtattta tatttatcat aagccaatat ggtgacctca aaggaagcca gcaatttagc
       61 gaacgcagac ccggtatttc cgaatccgat tataccgacg gtctttccgg tcagctccgt
      121 accccggtta gcatcgcgga tccatttccc ctccttcact tcctggaagg ctttgggcat
      181 tttgttcatt aaggaaagca acatgccgag tacatgttcg gccacggcat tccggttgcc
      241 ctccgggctg ctcacacatt gaataccctt cgattcagca aacggaacat cgatcagttc
      301 cataccgcta cccagccggc cgatccattt cagcaggttg gcccgctcca ggatattccg
      361 gtccaccttc aaccgggtcg tcacaatcag tccttcgata tctgcaacgg ccgtttccag
      421 ttcttcatac gttatttgag ggagatgaat aatctgatag cctcttgccg ataacctttc
      481 tatgaggtat tcatgaactt tcgccgtgat cagtaccttt ttcatccatt ttattttatg
      541 cggaacatcc agttcccttt aaccaatttc aatgcccgga ttgatgtgcc ctgcgcatca
      601 tctcattttc cacgtaagat ccgcaaattc gcggatcgta agttgttctg cccgccggtt
      661 aaataccggg tcttttaatg tgtcttcgga gaacaggccc tttacggcat tgcgcaacat
      721 ttttcttctt tgattaaagg ccgtttttac cagcaaaaaa aacaattttt ccgtttttgc
      781 cggaagaggt tctgttaatg gcgttaaccg gatgaccgca ctctttactt tgggcgccgg
      841 cgtaaatgcc tgttcgctta cttcaaacaa atattccacc tgataaaagg cctggatcag
      901 gacgctgatt actccatacg ttttatttcc ctctgcagcg gctatgcgct gggccacttc
      961 tttctgaaac attccgatca cccgttccac cgattctctc cagtctaaaa tcttaaacag
     1021 gatctgggtg gagatattat acggaaaatt gccgattacc gtaaagcgtc ccccaaaggg
     1081 catatccatt gttaaaaaat ccttttcaat cagatccgga atcaaggccg gataggtctt
     1141 tttaagaaag gccgctttct ccccatcgat ctccacggcc ctgaaatgaa tttctttaat
     1201 cccgatcagg tatttcgtaa gcgccccggc gccgggaccg atttccagta attgagaaaa
     1261 ggaatgttcc ccgagggcat cgacaatctg ccggcagacc gattcatctt ttaagaaatg
     1321 ttgtccgagc gatttcttta agttcatgga acgcggcagg tgggtcatga ctgcaaaaat
     1381 aaacgatccg gtcaggctaa taatcgtatt tttatccctt gaaaaattga gacatgagca
     1441 ttccgctaca aagacccgtg attggcattt ccatcggcga tacaaacggc atcggcaccg
     1501 agctggtaat aaagaccttt tccgataacc ggttgctgga actctgcacg cccgtgctgt
     1561 ttgcctccaa taaactcatt aatttttacc gcaaatccat tccggaatcc agtttcaatt
     1621 accagaacgt aaaagatctg tcccgaattt atcccaaaca ggtgaacatc ctcaattgtt
     1681 gggaggagga aataagcatt acacccgggc agctcaatga aacaggcgga aaatatgccg
     1741 taaaatcatt ggttgccgcc gtagaggccc tgaaggataa aaagatccag ggactggtaa
     1801 cagcccccat tcacaaaaaa aatattcaga acagtgaatt ctcctatacc gggcataccc
     1861 cctacctcct gcaggctttt gggatgaagg atgttttaat gctcatgagt tcggaaaatt
     1921 tcagggtagg actggtcacg gaacatgttc ccgtaagtga gatcgcaaaa cacattaccc
     1981 gggaagccat tctttccaaa ttaaatatta tgaaagattc cctgatccgg gatttcggca
     2041 tcgataaacc acgtattggg gtcctgggac taaacccgca cgcgggggat gaagggttga
     2101 tcggaaagga ggaggaagag atcatcaaac cggccatcaa ggatgccaaa cacaatatgc
     2161 tggtgtttgg cccgtacagt gccgatgcct tttttgcgca taaccagcaa cagaaatttg
     2221 acgcggtgct ggcgatgtat cacgaccagg gccttattcc gtttaaatcc ctggctttcg
     2281 gggaggggat aaactatacc gcgggcctgc cggctgtccg gacctctccc gatcacggaa
     2341 cggcgttcga tatcgccggt aaaaataagg cggatcatga atcctttatg gctgccgttt
     2401 tcgggtgcct ggctatcctg gataaacgac gcgatttcaa tgaaaaccac aagaatcccc
     2461 tgaagaaaat gggttcggtg gttcttgcgg gggctgaaga tgagaaaatc gatgaaggat
     2521 agcggggtaa tttaaaaatt aatcccccgg cctattgcaa ataccgaaac cgctttctat
     2581 ctttggaata cccttcccct aattccagat cgttacccac tgaactaatc catcccggat
     2641 tttctccaat agctatgtga acaattccga atcgttatcc gatcctttct tttgaatgac
     2701 ctctgttccc ctttacagct aggcatcatc acattgttca ctttctgaaa cacaaaaaat
     2761 gaaaaggatc atcaggttcg ccttgcctct tttactggca attacgggag ggttatttgc
     2821 ccatgctatt ccatctactg caacagatac agcaaacaac agcataagcc taacccttca
     2881 cggaagtctg gctgttttta atgcagctta cggccgaaat tattttgaac tgaactggaa
     2941 caccgtggcc ggtaatttcg atcacttcga aattgaaaga agcctggacg gacaaacttt
     3001 tgaaaaactc ggcgatgtga aagccggtga gatgggttca acaggccggt atatgttccg
     3061 cgaccatttt aaggcggtaa ccgcccggaa caatgatttc ttttaccgcc tgaaacagtt
     3121 tgatcccaac ggaaattcca gttactccaa agtgcttatt gccaggatgt ataatacccg
     3181 gtcgctggct tccctcagcg ttaccccgga tcccgtggcc aatgatatcc tggttaacgt
     3241 gcagctaaac caaaatgctt atgtaatgat gaagatactc gatgccgacg gcaatctggt
     3301 actgaaggaa ggacggaagg ccgataccgg ttccagtacc tactcgctga acgacaccca
     3361 taacctgcaa aagggaatgt acaccctgga agtaacggtg aacagcaggg aaaaattggt
     3421 tatgaaactc atgaaaggat agtttccggt aaccgctttt gccggatgcc catgccctct
     3481 gcgttaaaaa acggagggct ttttgtttcc cgttcagaga cttatgaata cacaaaagct
     3541 gccatcagct attcccgttt actgaaagat aggattgcag gcttttcacg tagctgtcaa
     3601 atgccttttt cccggctaag gtgatccggc acagggtgcg cggataattg tccttaaact
     3661 cctttaccac ttcaatatag ccggcctccc gcaatttatt gatctgtaca ctcaggttgc
     3721 cggcggtggc attggtctgc tcccgtattg cggtgaattc tgcctgctga tggctcatca
     3781 gcagggaaac tatggccagt cgcagttgcg aatgcagtac gggatcgagg tctttgaaca
     3841 ggttcatcag cattattaat tttgccggca agagaagggt gacaaattcc ggccttcata
     3901 aaaacgttgg ctaccgcgtc actggcactt ggccagttct tcttcggccc tggcctggcc
     3961 ccaatcgggg tatagtggtt tgggctgctg ttttttaaat aattcaatcg ctttttcaaa
     4021 aataggcttt gcttttgctt ttccgccacc gaaggcttcc ggtgtattca tgacgctttc
     4081 cccctgcaga taatacaagc gggggttatc cggatccagt ttaagcccgc tttgcagggc
     4141 agcggatgcc tgctggccgt aggtttgcca ccgcgtctgc ggatcaacca tcatttgttg
     4201 cgtagccgac atattgcgga gcgcataaat ttcggcattt ttttcaatgg cttctgcctt
     4261 gtcgcagagc gcattgatcc gggttgaatt agcgtctttg tcgagtttcg gatcgctcca
     4321 tccggcggtg gagagcgcca gtccggcata ataatacggc agccattgtg ttttttcagc
     4381 atccccgatg cgttcaaagg tattggccac cgactggtaa tctgccgtcg tttttgccgt
     4441 tccgaactgg gccaggtttt tcttcatagc ctccgtataa cggtcgcttt gggccatgct
     4501 gccgatggca gcgaacagga atagagagaa aagaattgat ttcatataag gataattaaa
     4561 aagtgacaat tacagattgt tattaatggc atcctgggtc cggtccacac cccagctcag
     4621 gaaacagcct ataaaataaa accggttggc cggcggctca atcgcctgct tttgctgacc
     4681 gttgaaagaa taattgtacc catacaccgc gttatatccc agcacattgc tcatgctcgc
     4741 aaaaaggacc acgaatgttt tgggatttgt ttttccgata cttggaacat attccatgct
     4801 gaaattgagg ctgtgataat ccttggtata cccctgatcg gcggtatagt attttccgga
     4861 gttgttgatc ataaaatcat aatagggtct cccgctggca taggtatagg taagattaaa
     4921 tccggtcttc cagtccgtaa aaaatcgttt catcaccagg gaaaccgtat tcttggcagc
     4981 aaaattcggt gtgatcaaac ccgggtaatt cagataatcc ctcttggtat ccagatagga
     5041 ataacttatc caataatcga aattcttgaa ggtcttctta tccctccaga aaaattcaat
     5101 ccccctggca tccgccctgc cggtattatt ataattaaaa taataataat tgaccggaac
     5161 ggtcttgatc aggtctttat atttcttata ataagcctcc acccggaaga tctgatccct
     5221 ggtcatcttc tgaaagttga taatataatg tgttgcctgg gtgaatccca gatcggaagt
     5281 ataaaacaat tgctgatttt ctgccttctg gtaaaagata ccgtaggccg cggaaatctg
     5341 ggcatccttt ccggtcttat aggccaggga tatccggggt gcgagatcgg attttctgat
     5401 cagggacgaa tgctcatacc gcaggcctgc tttcacggca agttcgttcg taagataaat
     5461 atcggtttca gcaaatccgg caagcatatt gtccttaagt ctccgggaag tatcctgata
     5521 aacaaagggg ttattattgt accagtattc ggccccgaac cggaaagcgc tgagaccgcc
     5581 cagttttttc tccaatatcg ccttggtctg ggcaagatcc tcgcggttct tcaaaacaaa
     5641 atttttgtta ctcatccaga acgtgcttcc cgtataatag tgaggccggt tatccggacc
     5701 ttccacctgc tggttgatat ggtccaggtt ggtactgtag ctgccgctga gattcatttt
     5761 ccatccgttc cttaaatatt cccgccagct cagattatta taccagttgt gattgcccag
     5821 gtcaaaggca tttttcagat aggaactatc cacatccggc cttctcaaac caagttcatt
     5881 ataattgaaa gtggtataat acttgatcat ccccccgttt ttcgttttga cccgaaaatt
     5941 ggcgtcgccg gaatgaaact ggggaagcct gaaataatcc ggggtctgtt tcacgattcc
     6001 gaaatagacc cccacgttca catagttgta tgaaattccc caggaagatt ttttattttt
     6061 tgccagctgc tgcgtgccca cacctacgac gatcggtgaa atggaggcat ccacttccga
     6121 tttttcaggc agatcgatgg attcgagtaa tacgacggat gaaagagcct gtccgtaaag
     6181 agccgaatac ccgcccgtgc tgaaaaccgt tcccttaaaa agaaatgggg aaaagcggcc
     6241 gcgggtggaa atatcgggaa cactggtata atagggattg ttcaccaggc tgccgtcgat
     6301 aaactgtttg gtttcctggc tggttcctcc acgaacaaaa agtccttcct gttccccgac
     6361 ctgctgggct cccggcagcg ttttcaatgc ggccgtgata tccgcattgg aaccggccgt
     6421 ggtggccaca tccagggaac tcaataccgc cccgcgtttg gagtcgcccg cagaaaaaga
     6481 gcctgcggta atcgtaacgg ccttcatttc attaggctct tcctttagct gaagatcaag
     6541 ataaaccgcc tcacccttta acaaaatggg ctgaaccagg ggccggtagc cgatggaggt
     6601 gaccgtgatc tgctgattgc cggtgtccga tgcggaaaac cggtaattcc cggaagaatc
     6661 cgaagtggct ccgtcaaaac tgtttttaat ggtaatgctt gccccgggca cgggatgtcc
     6721 ccttccatct ttaattttgc cagatacgat cgtctgcgaa aaacctgtct gcaggcagaa
     6781 aaggattaaa aataataacc ccgtattctt aaaacgttgg tttttataat gttgcgtttt
     6841 aaagcaggag aacatagcct tggttttgta aatttgacgg atagccgctg gccggaatac
     6901 acaaatgtaa agtagtttat aatataaacc aaaatatttt ttaacgggcg ggttggtaac
     6961 aaccggttga taaaaaccct tcgaccgccg aacaagaccg tctatttttg ggttgttaaa
     7021 aaaaggatct ttcacttaag cgattcccat gagatggagt aacacggaaa atgaaaaaca
     7081 taccattgaa cgctttgacc ttttggatgg tcattccctg gagatgacgc ttcgctataa
     7141 ccccattcag cactccggcc ggttggaacg caataacaac cacctgcttt tttttatcga
     7201 acaaacaggt ctctggggta acaagttctt gtttgagaat gaatatgggg tggagatcgg
     7261 caggatctct tttgacaacc tgcaaaacct cgacggcagc atccatatcg acagaaagaa
     7321 actatctttt aaaattcccc tgaaccgttc cggggatgtt ttcttcgttc ccaataaatc
     7381 ccttaaacag aatttccatt gcagcattga tgccgggagt attttatccg gaccggataa
     7441 aaaagtcagg acagagcaat cagaaaaata tgcctgttta attctcggcc tgttctggta
     7501 tctttttccg gaatccattc cggaagccaa taaatcaggc gtttcgattg cgctgcatta
     7561 agacctatcc aaaaaaatct gtcaggtaaa aacaaaaggc ccggcttaga aaagccaggc
     7621 cgttgttgat cgtacccttt aaaactaaaa cttaagaatt cgtttttata aatttttgta
     7681 ccgcggtttc ttttccggtc gccgtctgga taatatatac tccggcggcc aggtccgcca
     7741 cctgcattgt ttctgtttgg cctgcattca cgctcagcag ctgtctcatc aaaacgccat
     7801 ttgaattaaa taatttatag ttaaccgttt tattttgcca ggaatcggga atggtaatcc
     7861 tcaattccct ggccaccggg ttgggataga gcatcagtct ttgctcttca ttcttaacct
     7921 ttaccacaat tacatcagaa taccggaatt ttgaatccag atca
//
LOCUS       DASYCU010000139         5905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1340788, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000139 DASYCU010000000
VERSION     DASYCU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340788"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..518)
                     /locus_tag="VGM24_02760"
     CDS             complement(204..518)
                     /locus_tag="VGM24_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154310.1"
                     /translation="MRRGFMELCLLIVVIIGIHAERAMHSGSIEGSVYPSNLTPSVVA
                     VQGSDSVKAFSNDGHFGMRLQPGVWKLIFAVKYPSQSLVEKNVNISEGQRINLGEIKF
                     AE"
     gene            complement(685..1857)
                     /locus_tag="VGM24_02765"
     CDS             complement(685..1857)
                     /locus_tag="VGM24_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEY4154311.1"
                     /translation="MSTYLLLRNNKESGPYTIDELKRMSLKPFDLVWIEGKSAAWRYP
                     GEIPEFSSFAPAAPEQPFDRFYKKPVPESAASTGRDDGYRKPPVVNQKRENRESGNAH
                     RESVYINLPSSEKKIPALIPVVQAWNPIDLSWEEPVRSVPVKTVGRQGKKKQKWLVIG
                     AVILFFGAGMSTGFFISNRRIFYSEDGNPPQHPTSPAKVLTSTQTGSEQPEIFSGAGG
                     SKGPGSKDSSIPPERNHQSGSKKRQVISGLGEKDSLYTASAPAPAPFIPDSVREREDH
                     ENKIAALAAKIKSHPEEYLLISTGNYKVGVLGGISGFPVSITNRSGVSLDLVVVAVDY
                     ILHNKKIFKTENLSFHNLAPNATVSGEAPKSSRGVRITYRLTIANAQQIGMAYSNL"
     gene            complement(1963..2916)
                     /locus_tag="VGM24_02770"
     CDS             complement(1963..2916)
                     /locus_tag="VGM24_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HEY4154312.1"
                     /translation="MKIFFPCHFKCRNTLMRFIFILVFLCEFLCGHSQNGFLPPADRY
                     KANRMRTVIITEVASGVAISAGLYFLWYRKHPRSRFHFFNDNSEWLQMDKAGHATTAY
                     NIGSIQYDLMRWCGVKKDASIAISSVTALGYMSIVEVLDGFSSKWGFSRGDMLANIFG
                     TALFAFQQKAWNQQRISLKFSFHPTRYAQYYPSELGNNLISRMLKDYNGQTYWLSFNI
                     ASFLPAKTDFPYWLNLDLGYGAEGMIGAYSNPDSVKGVKIPDFPRYRQFYFSPDADLF
                     RIPSSSAIYNSASYLTRFSKLPAPTLEWSRLKGLGFHPFYY"
     gene            3007..4734
                     /locus_tag="VGM24_02775"
     CDS             3007..4734
                     /locus_tag="VGM24_02775"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEY4154313.1"
                     /translation="MDAAILAKVNSWLNGNYDEETKTAIARLQKENPQELTESFYKNL
                     EFGTGGLRGIMGVGTNRINKYSIGLATQGYSNYLKKSFPSQQVKVAIAHDSRNNSRFF
                     AETAAKVFAANGIKVFLFQSLRPTPELSFTIRYLGCQGGVVCTASHNPKEYNGYKAYW
                     NDGGQLVPPHDHNVIAEIEQIGSVDEVKWTGGEPHITMIGKEMDEAYISMVKDLSVNP
                     EIIELQHNLCIVYTPIHGTGIKLVPDTLQRFGFSNVHVVDEQATPDGNFPTVAFPNPE
                     EKETMSLGLKKAREINADILLGTDPDADRVGIGIKNDKEEWILLNGNQTAVLAFNYLI
                     EARKEKGISQPNDMVVKTIVTTDMIDEIARASNIRCYNTLTGFKWIAELIKEKERSEN
                     YVIGGEESYGLMIGSKLRDKDAISAVAILCEMTAYEKNKGKTLYEKLIDLYLRFGFYK
                     ESLISITKKGMDGQKQIADMMAAYRKNPPSVINGSAVIQLLDYETGRGTNLKSAETWK
                     IELPASNVLQFILEDGSKISARPSGTEPKIKFYFSVHDKLKDRSSFHMLDDLLQKRID
                     SIISDMKLK"
     gene            4780..>5905
                     /locus_tag="VGM24_02780"
     CDS             4780..>5905
                     /locus_tag="VGM24_02780"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY4154314.1"
                     /translation="MKTKWFCLIGLLISVSAHSQVRLAKIFGDHMVLQRSQPIPVWGW
                     ASPNEKITVTLNNGFRDTRADKNGKWRLNLDPQPAGGPYDMKVAGKDSLVLHDVLVGE
                     VWICSGQSNMEFQLRSALHADEEIANANYPQIRQIKIPNTASLTPKEDIPSGEWTVCS
                     PATAADLTAVGYFFAREIQNRIHVPVGLINSSWGGTMVETWTSRGAFEKSPEFKSMIA
                     GLPATSVETLAKQRRQDTEEKVRLMEKGITDSVPENQWKNPDYNSDAWPKMKIPGLWE
                     SWNAGLEDLDGIVWFRKLITLDDAEAAKPVILSLGKIDDNDQTFVNGTPVGSTRSYSA
                     VRHYTVNPGIFKAGGNVIAVRVEDTGGGGGIYGDSADLNLI"
BASE COUNT         1627 a         1394 c         1472 g         1412 t
ORIGIN      
        1 tccgcattgg cgtcggcaac ggttaatgtc cattgattcc gggatcccgt aatggaaaga
       61 tactctatca ggcaggtggc agatttcccc gcaccaaaaa gcaggatatt attcattatc
      121 caatgataaa gcatttcccg caaaaaaaaa gtcagcccgg gaaaagccgg gctgacctcg
      181 aattaccatc ttttgccgat atcttattca gcgaatttga tctccccgag atttatccgc
      241 tgtccttctg aaatatttac atttttttcg accaggctct ggctgggata tttcaccgca
      301 aatatcagct tccacacgcc gggctgcagc cgcatcccaa aatgaccgtc attactgaaa
      361 gccttcaccg agtcacttcc ctggacagcg accaccgatg gtgttaaatt cgaaggataa
      421 acacttccct caatgcttcc actgtgcatt gcccgttctg catgaattcc gataatcaca
      481 acaatgagca ggcaaagctc cataaatcct cttctcatct gggtaaatta aactgttact
      541 gataaatctt aaattctttc tctataacca atcaatgcca ggcgttggag ttacacagga
      601 tttgaatcgg acataactct tcttttcaac ttttatgcca agggaaaatt tttcggataa
      661 aaccaaacaa taaatttcca ttaactacaa attggagtaa gccataccaa tttgctgcgc
      721 attcgcgatg gtcaaccggt aggtgattcg tacgccgcgg gagctttttg gtgcttctcc
      781 gctaaccgtg gcattggggg ccaggttgtg aaagctcaga ttttccgttt tgaaaatctt
      841 tttgttatgc agtatataat caacggccac aacaaccaga tccagggaaa cgccggaccg
      901 gttggtaatg gaaaccggga acccggagat tcctcccaat acacccactt tataattccc
      961 ggttgaaatg agcaggtatt cttcgggatg ggattttatt ttagctgcca gggcggctat
     1021 tttattttcg tgatcctctc tctcccggac agaatcgggt atgaaaggag cgggagccgg
     1081 cgcagaagcg gtataaagcg aatctttttc tcccaaacca ctgattacct gcctcttttt
     1141 ggaccccgac tgatgatttc gttcaggtgg aatggacgaa tctttggatc cgggtccttt
     1201 gctaccgccg gctcctgaaa atatttccgg ttgttcacta cctgtttgtg tgctcgtcag
     1261 gacttttgcc ggagaggtcg ggtgctgggg aggattcccg tcttctgagt aaaaaattct
     1321 acggtttgag atgaaaaatc cagtggacat acctgctccg aaaaaaagga tcacagctcc
     1381 aatcaccagc catttttgtt ttttcttccc ttgtctgccc accgttttaa ccggaactga
     1441 ccggacgggt tcttcccagc tcagatcaat tgggttccag gcctgcacaa ccgggatcaa
     1501 tgcgggtatc tttttttctg aagaaggcag attgatatat accgattccc ggtgggcatt
     1561 ccctgattcc cggttctctc ttttctgatt gacaaccggc ggtttccggt atccatcgtc
     1621 gcggccggta ctggcagcag attccggaac gggcttttta taaaagcggt cgaatggctg
     1681 ttccggtgct gcgggggcaa atgaactgaa ttcaggaatt tctccggggt aacgccaggc
     1741 tgcactcttc ccttcaatcc ataccaggtc gaagggtttc agggacattc gtttgagctc
     1801 atcaatagtg tatggtcctg attctttatt gttccgcaac aataaatacg tgctcataat
     1861 ccagggaata aaaggttagg gggtactgac cagagatctt cccaaaagta ccgacttttt
     1921 ttgtgaagcc aacgttataa accgcaatgt ccggggtttt attcagtaat agaaagggtg
     1981 aaaacccagg ccttttaacc tgctccattc cagggtcggc gcgggaagtt ttgaaaacct
     2041 ggtcaggtag cttgcactgt tataaatagc ggaagaggaa ggtatccgga acaggtcagc
     2101 gtccggagaa aaatagaact gccggtagcg gggaaagtcg ggaattttca ctccttttac
     2161 cgagtccggg ttgctgtaag cgccgatcat tccttcggcg ccataaccca gatccagatt
     2221 caaccaatag ggaaaatccg ttttagccgg cagaaaggaa gcgatattga aagaaagcca
     2281 gtaggtctga ccgttataat cctttaacat ccgggaaatc aggttatttc ccagttccga
     2341 cggataatac tgggcgtagc gggtcggatg aaaagaaaac ttcagggaga tccgctgctg
     2401 attccaggcc ttctgctgga aagcaaataa agcagtaccg aaaatattgg ccagcatgtc
     2461 ccccctggag aaaccccatt tggaagaaaa accatccaat acttccacga ttgacatata
     2521 ccccagggca gttaccgaac taatggcgat ggaagcatct ttttttaccc cgcaccacct
     2581 catcaggtca tactgtatgc tgccgatatt gtaggcggtc gtggcatgcc cggccttgtc
     2641 catttgaagc cattccgaat tatcattgaa aaaatggaac cggctgcggg gatgtttccg
     2701 gtaccataaa aaatacaaac cggcagatat ggccacgccg ctggccactt ccgtaataat
     2761 caccgtacgc atccggtttg ccttgtagcg gtcagccgga ggtaaaaagc cgttttggga
     2821 atgaccgcac aaaaattcac acaggaagac cagtataaaa atgaaacgca tcagcgtgtt
     2881 ccggcactta aaatggcatg gaaaaaatat tttcaatccg aaagaatttt aacgggcgct
     2941 aaccttaaag gaagttttgt ttcgtacaaa aatctctacc ttaggttcaa atttaaagca
     3001 agttttatgg atgcagccat tctggccaaa gtgaatagct ggttgaacgg aaattacgat
     3061 gaagaaacca aaacggccat cgcccggctg caaaaggaga atccgcagga gcttaccgag
     3121 agcttttata agaatctgga attcgggacc ggtggtctgc gtggaatcat gggggtgggt
     3181 acgaaccgga tcaataaata cagtattggc ctggccaccc agggttactc caattacctg
     3241 aaaaaatcct tcccgagcca gcaggttaaa gtggccatcg cgcatgacag ccggaacaac
     3301 agccgttttt ttgctgaaac ggcagccaaa gtatttgcgg caaacggtat taaggttttt
     3361 ctttttcagt ccctgcgacc tacgccggaa ctcagcttta cgatccgtta cctgggatgc
     3421 cagggcgggg tggtttgcac cgcttctcac aatccgaaag aatacaacgg gtacaaagcc
     3481 tattggaatg atggcgggca actggtgcct ccgcacgatc acaatgtgat tgccgaaatt
     3541 gaacagatcg gatccgtgga cgaagtaaag tggacaggcg gggagcccca tatcactatg
     3601 atcggaaaag aaatggacga agcctatatc agcatggtca aagacctctc cgtgaatccc
     3661 gagatcatag aacttcagca taatctctgc atcgtttata ctcccattca cggaaccggt
     3721 attaaactgg ttccggacac tttgcaaagg ttcggattca gcaatgttca tgtcgtggat
     3781 gagcaggcaa cccccgacgg caattttccc accgtggcct ttcccaatcc ggaggagaag
     3841 gaaaccatga gcctgggatt gaaaaaagcg agggaaatca atgcggatat tctgctgggc
     3901 accgatccgg atgccgaccg ggtggggatt ggaataaaaa acgacaagga ggaatggata
     3961 ttattgaatg gtaaccagac agccgttctg gcattcaact acctcatcga agcccggaag
     4021 gaaaaaggga tctcccagcc caacgatatg gtggtaaaaa ccatcgtcac cacggacatg
     4081 attgatgaaa tcgccagggc aagcaatatc cggtgctata acaccctgac aggattcaag
     4141 tggatcgccg aactgatcaa agaaaaagag agatcggaaa attacgtgat cgggggggag
     4201 gagagttatg gactgatgat cggctccaag ctgcgggaca aagatgcaat aagtgcggtg
     4261 gctattctgt gtgaaatgac cgcctatgaa aaaaacaaag gcaaaaccct gtacgagaaa
     4321 ttgattgacc tctacctccg gttcggcttt tataaggaat ccctgatctc gatcacgaaa
     4381 aaaggaatgg acgggcaaaa acagattgca gatatgatgg cggcttaccg taaaaatccg
     4441 ccatccgtca tcaacggctc tgccgttatt caattgctgg attatgaaac cggcagggga
     4501 acgaatctga agagcgcgga aacctggaag attgagcttc cggcaagtaa tgtgctccag
     4561 tttatcctgg aggacggaag taagatttcc gcccgtccgt ccggcaccga gccaaaaata
     4621 aaattctatt tcagcgtgca tgataaactg aaagacaggt cttcttttca tatgctggac
     4681 gacctgcttc aaaagagaat agattccatc atcagcgata tgaagctaaa ataatccggg
     4741 gcagcgccta tttcttcccc ggtataatac agcctgaaaa tgaaaacaaa atggttttgc
     4801 ctgatcggtt tactgatatc cgtttccgct cattcccagg ttcgcctggc aaaaattttt
     4861 ggagaccata tggtgcttca gcgaagccag cccataccag tttggggatg ggcgtcgcca
     4921 aatgaaaaga tcacggtcac cctgaataac gggttcaggg atacccgggc ggataagaac
     4981 ggaaaatggc ggctaaacct ggatcctcag cctgccggcg gaccttatga tatgaaggtt
     5041 gccggtaaag acagcctggt attgcatgat gtgctggtgg gtgaagtatg gatctgctcg
     5101 ggacaatcca atatggaatt tcagctaagg tccgccttgc atgcggacga agagatcgcg
     5161 aatgccaatt acccgcagat ccggcagatc aaaattccga atacggccag tctgaccccg
     5221 aaagaggaca ttccttccgg cgagtggacg gtttgttcgc cggcaactgc cgctgacctc
     5281 accgcggtcg ggtatttctt tgcccgggag attcaaaatc gtatacacgt gcccgttgga
     5341 ctcatcaatt cctcctgggg aggaacgatg gtggaaacat ggacaagccg cggcgctttt
     5401 gagaaaagcc cggaattcaa atccatgatt gccggccttc ccgccacgag tgtggaaacc
     5461 ctggccaaac aacgcaggca ggacacggag gaaaaggtcc ggttgatgga aaagggaatt
     5521 accgacagtg taccggaaaa ccagtggaaa aacccggatt ataacagtga tgcatggccg
     5581 aagatgaaaa tacccggact ctgggaatcc tggaacgccg ggctggaaga cctggacggg
     5641 atcgtctggt tccggaagtt gatcaccctg gatgacgcag aagccgcaaa accggtcatc
     5701 ctgagcctgg ggaaaattga cgataatgat caaacctttg tgaatggaac acctgtcgga
     5761 tcaacccggt cttattctgc cgtcaggcat tatacggtca atccgggtat ttttaaagcg
     5821 ggcggcaatg tgattgccgt acgcgtggaa gatacgggcg ggggaggcgg aatctatgga
     5881 gattctgctg atctgaacct gatag
//
LOCUS       DASYCU010000140         3719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2790319, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000140 DASYCU010000000
VERSION     DASYCU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790319"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..788)
                     /locus_tag="VGM24_02785"
     CDS             complement(153..788)
                     /locus_tag="VGM24_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY4154315.1"
                     /translation="MAKTKKPKHIAVAGNIGAGKTTLTELLSKHYKWIPQFEDADHNP
                     YLNDFYEDMPRWSFNLQIHFLNGRLNQLLEIYTGTETVIQDRTIYEDAHIFAPNLHDM
                     GLMSKRDYNNYFQFFQTLKRMVQPPDLLIYLQASVPTLVGQIQKRGREYEENIRLDYL
                     KRLNELYNKWIDGYKEGGLLVIDVDKNKFPENEEHLGEIITRIDGQLYGLF"
     gene            complement(848..1843)
                     /gene="trpS"
                     /locus_tag="VGM24_02790"
     CDS             complement(848..1843)
                     /gene="trpS"
                     /locus_tag="VGM24_02790"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY4154316.1"
                     /translation="MEKKKEIVLSGIRPTGFLHLGNYFGAVRNWARMQYEYDCYFFIA
                     DWHSLTTHADTKELKENTRRLVSELVAGGLDPNQCVLYAQSDIPEIAELYLYLNMLAY
                     RGELEKTATFKDKVRQQPQNVNAGLLTYPVLQAADILIHRALKVPVGKDQEQHVEMTR
                     NFAERFNHRYGNVFPLPVAFNYGNDLLKIMNLDGTGKMSKSENQMNTIYLNDEDALIT
                     KKIMKAKTDQGPAEPNSKKPDYIQGLFTLLSLVSKPDTVEKFETDFNECRIRYGDMKK
                     HLAEDLVRFIRPLREKAQSLQQNPAEIDRILAEAAGKARKSAGATLLKVREAMGF"
     gene            2120..2419
                     /gene="gatC"
                     /locus_tag="VGM24_02795"
     CDS             2120..2419
                     /gene="gatC"
                     /locus_tag="VGM24_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY4154317.1"
                     /translation="MEVNDALIEHLCHLANLEFDENGKQEIKADLEKMIGFVEKLRAL
                     DVSGVEPLLYMGDETDVLREDILEPSISRPDALKNAPLADEQYFKVPRVVKTPSA"
     gene            2446..3159
                     /locus_tag="VGM24_02800"
     CDS             2446..3159
                     /locus_tag="VGM24_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4154318.1"
                     /translation="MSAQTIILLDRIQKSYFMGKQAVPVLKGISLEILKNEYVALMGP
                     SGSGKSTLMNILGCLDSPTAGKYILNDQDVSEMEDDSLAEVRNKEIGFVFQQFNLLPR
                     LTAAENVALPLVYAGLPKKTRMELAMEVLEKVSLTDRSHHKPNELSGGQCQRVAIARA
                     LVNNPSLILADEPTGNLDSKTSIEIMEIFNKIQAAGNTVVLVTHEEDISNYAHRVVRL
                     KDGIIETDKRREGVFAPAL"
     gene            3172..>3719
                     /locus_tag="VGM24_02805"
     CDS             3172..>3719
                     /locus_tag="VGM24_02805"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY4154319.1"
                     /translation="MSVKIYTKTGDAGKTSLLGGSRVAKDSIRIEAYGNIDELNSFIG
                     LLSDQYSDAPSRAVLKEIQDRLFTIGSALACDPEKEIALKIPDLKEEDVFLLEKEIDR
                     MSAVLPPMKFFILPGGHVVVSTAHICRCVCRRAERAAVRLNAEQPVQPLILKYLNRLS
                     DYLFMLARYAGLQLGVAEVSWQP"
BASE COUNT          921 a          891 c          927 g          980 t
ORIGIN      
        1 gtttcattga aaattttaag aaatatgaaa gccaggttcc ggaagaaatt ttaaggggag
       61 cgccggagat ctgaaaaatg aaaaaccgcg gaaatcaaaa tatatccggc atttcgaaac
      121 gggatggtaa tttaattccc gggactccgg tcctaaaaca acccgtatag ctgtccgtca
      181 atccgggtga tgatttctcc caggtgctct tcattttccg ggaacttatt tttatcgacg
      241 tcaatcacca ggagcccacc ctctttatat ccatcgatcc atttattgta cagttcattt
      301 aaacgcttca ggtaatccag gcggatattt tcttcatatt cgcggcccct tttctgaatc
      361 tgtcccacca gggtaggcac cgaggcctgc aaataaatca gcaggtcggg tggctgcacc
      421 atccgtttca gggtttgaaa aaactgaaaa taattattat aatcccgttt actcatcaac
      481 cccatatcat ggaggttcgg tgcaaaaata tgggcgtcct catagatggt gcgatcctgg
      541 atcacggttt cggtgccggt gtagatctcc agcaactggt tcaggcgccc gttcagaaaa
      601 tgtatctgaa gattaaagct ccagcggggc atgtcttcat aaaaatcatt caggtaagga
      661 ttatggtcgg catcttcaaa ctgagggatc catttgtagt gcttgctcag caattcggtc
      721 agggtggttt ttccggcgcc tatattgcct gccacggcaa tatgctttgg tttcttcgtt
      781 tttgccatgc ctaaatgtaa agtcttatca tggaaatata aaaagaatcg ttgaggtgga
      841 aacggtatta aaaccccatt gcctcccgta ctttcaacag ggttgcaccc gcacttttcc
      901 ttgccttccc tgcggcctcg gccaggatac gatcaatttc ggcagggttt tgctgcaggc
      961 tttgggcctt ttctctcaag ggcctgataa agcggacgag gtcttcagcc agatgctttt
     1021 tcatatcgcc ataccggatc cggcactcgt tgaaatcggt ttcaaatttt tctaccgtat
     1081 ctggtttact aacgaggctc aggagggtga acagcccctg gatatagtcc ggctttttgc
     1141 tatttggttc tgccggtccc tgatccgttt tagccttcat gatctttttt gtgatcaggg
     1201 catcctcatc attcaggtaa atcgtattca tctggttttc gctcttgctc atttttccgg
     1261 ttccgtccaa gttcatgatc ttcagcagat cgtttccata attaaatgca accggaaggg
     1321 gaaatacatt tccataccgg tgattaaacc gttctgcaaa attccgggtc atctcaacat
     1381 gctgttcctg gtccttacct acaggcactt tcagtgcacg gtggattaaa atgtcggcag
     1441 cctgcaaaac ggggtaggtc agcaggccgg cattcacatt ttgcggctgc tgcctcactt
     1501 tatccttgaa cgttgcggtt ttttccagtt cgccccggta agccagcata ttcagataaa
     1561 gatagagttc ggctatctcg ggaatatcac tctgggcgta cagcacgcac tgattcggat
     1621 ccagcccgcc ggcaaccagt tccgatacca gcctccgggt attctcctta agctctttcg
     1681 tgtcggcatg ggtggtcaga ctatgccagt ccgcaataaa aaaatagcag tcatattcat
     1741 actgcatccg ggcccagttc cgcacagccc caaaataatt tcccaggtgc agaaaaccgg
     1801 taggtctgat cccgctcaat acgatttctt tctttttttc catgaggagg acgaaggtaa
     1861 taaaacgggc ggaaagcggg ggccggggga agagtgtaac agcccgggct ccccatcccg
     1921 gcttaggggt tacctgccat cggggactgt cctccgcccg gtttcgatga tttttaccgc
     1981 ccggacgaag aaacaaaaaa atttaacttt tctatccggt ttccccatcc ggggatcctc
     2041 gcagaagggg ttttgtaata agcgttccgg gttatgtgtt tcgccttgaa tccgttattt
     2101 ttgtctctaa tcccttttga tggaagtcaa tgacgcgctt atagagcatt tgtgccatct
     2161 ggccaacctg gaatttgatg agaacgggaa gcaggaaatc aaagcagatc tggaaaaaat
     2221 gatcggcttt gtggagaagc ttcgtgcgct ggacgtatcc ggagtggaac ctctgcttta
     2281 tatgggggac gaaacggacg tgctgcggga agatatttta gaaccctcca ttagccgtcc
     2341 ggatgcattg aaaaatgcgc cgttggcgga cgaacaatat ttcaaggtac cccgagtagt
     2401 taaaacccca tccgcttaac cggagcaaaa tgaaataacc attctatgag tgcgcaaacg
     2461 attattcttc tcgacaggat tcaaaaaagt tattttatgg ggaagcaggc ggttccggta
     2521 ttaaaaggga tcagcctgga gattttgaaa aatgaatatg ttgccctgat gggtccctcc
     2581 ggttccggga agagcacact catgaatatt ctcgggtgcc tggattcccc gactgccgga
     2641 aaatatattt tgaatgacca ggatgtcagc gagatggagg acgacagcct ggcggaggtc
     2701 cgcaataaag aaatcggttt tgtttttcag cagttcaatt tgctgccccg gcttaccgcc
     2761 gcagaaaatg tggccctgcc cctggtttat gcgggcctgc ccaaaaagac ccggatggaa
     2821 ttagcgatgg aggtgctgga gaaagtgagc cttacggata gaagtcatca taaaccgaat
     2881 gaattgtccg gcggccagtg tcagcgcgtg gccattgccc gggcgctggt gaataatcct
     2941 tccctgatcc tggcagatga accgacggga aacctggatt cgaaaacctc catcgagatc
     3001 atggagatct ttaataaaat tcaggctgcc ggcaacacgg tcgtactcgt aacgcacgaa
     3061 gaagatattt ccaattatgc tcaccgggtg gtgcggctga aggatgggat catcgaaacc
     3121 gacaagagaa gagaaggagt atttgcgccc gcactttaat ttttttgcgg aatgtctgtt
     3181 aaaatctata caaaaacagg ggatgccggc aaaaccagcc tgctaggagg gagccgggtc
     3241 gccaaggata gtattcgcat tgaagcgtat ggaaatatag atgagctgaa ctcgtttatc
     3301 ggccttcttt ccgatcaata ttccgatgcg cccagccgcg ccgtgttaaa agaaatccag
     3361 gaccggcttt ttacaatcgg ttcggcgctg gcctgtgatc ctgaaaaaga gatcgcatta
     3421 aagatccccg atttgaaaga ggaagacgtg ttcctgctgg aaaaagaaat tgaccggatg
     3481 agcgcggtgc tgcctcccat gaaattcttt attctgcccg gtggccatgt ggtggtatcc
     3541 acggcgcata tttgccgttg tgtttgccgc cgcgccgaac gcgcggcagt gcgactgaac
     3601 gcagagcaac ccgttcagcc actcatcctc aaatatctta accggctcag cgattacctg
     3661 ttcatgcttg cgcgttatgc gggtctgcaa ctgggggtgg ccgaagtgtc ctggcaacc
//
LOCUS       DASYCU010000141         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1341109, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000141 DASYCU010000000
VERSION     DASYCU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341109"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VGM24_02810"
     CDS             <1..809
                     /locus_tag="VGM24_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4154320.1"
                     /translation="INLQQHAFPRGWGGVFASLPFAIWFFLGIEGVANVAEETQNPQR
                     TILRGFGSAILTLVILCVLTFAGSVGVAGWEAVVFKADGTDSDSPLPLALSHVVSNNH
                     ILYHLLITVGLLGLVASFHGIILAAGRSSFEFGRVRFAPAWLGKIHARFHTPVNALLA
                     NMVIGIVALCTGKTAEIITISVFGALTLYILSMISLFRLRKNEPGLDRPFKVPGYPAS
                     PLVALIIAGISFIAMTFYNFTLAIVYCGLLAGSYILFKLFSRLKSSPGQH"
     gene            824..2182
                     /locus_tag="VGM24_02815"
     CDS             824..2182
                     /locus_tag="VGM24_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY4154321.1"
                     /translation="MDPKEILHYVKAHPAGKVKIAFSDMDGVLRGKYISTEKFLSVVE
                     HNSSFCDVIFGWDMGDAAYHNVTYTGWHTGYPDAPARIDLNSFRKIPWEDGLPFFLGE
                     MLDRDGKAADICPRQLLKKVLKEAQDLGFTPYFSQEFEWYNFAETPQSATEKQFRNLQ
                     PLTTGMFGYSILRTSLKNDYFTDLFDLLKKFDLPLEGLHTETGPGTYEAAIGYASLPE
                     AGDRAILFKTAVKEIAYRHGIMASFMAKVNENLPGCGGHIHQSLWDKSSGKNLFYEEK
                     DPLKMSRIMRQYTAGQLYCLPDLSAIYAPTINSYKRLVEGGWAPTTLTWGIDNRTVAL
                     RVLNHSEKSCRLETRVVGADVNPYLAMAAALASGLYGIKNQIDLRQPPTNGNGYLDTA
                     NGHLPHTLEQATARMKQSSAPGELLGEIFINHFIQTREWEWKQHLKAVTDWELRRYFE
                     II"
     gene            2221..3369
                     /locus_tag="VGM24_02820"
     CDS             2221..3369
                     /locus_tag="VGM24_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY4154322.1"
                     /translation="MRFDHITQFNFPTLIRFGPGASKELPDYLKKNRLERPLLVTDPV
                     IAGLKFFKAIVADLETNHLGVEVFSGIHKNPVKSDVYAGTAMYDQTRRDSVIGIGGGA
                     ALDVARAIVLRVNHREDLFKYDDLVGGDVFVTNDVPHFITIPTTAGTGSEVGRSAIIA
                     DDATHQKKILFSPKLLAKVVFADPVLTLDLPAFITAATGMDALTHNMEAFLAKMDHPM
                     CEGIALEGMRLISQSLEKAVNRPDLVSRSEMLLASLMGAVAFQKGLGVVHSLAHPLSS
                     LLDTHHGLANAVNLPYGMEFNREGFEEKFRRMAVALELSGDRENEVVTYISDLNEKIG
                     IPERLGSIGVKQEHVETLADLAFADFAHPNNPKPVSREDFKKLYQQAL"
BASE COUNT          832 a          881 c          915 g          830 t
ORIGIN      
        1 tgattaatct gcagcagcat gctttccccc ggggatgggg cggtgtattt gcttctcttc
       61 catttgcgat ctggtttttc cttggcattg aaggagtggc caacgtggcg gaggaaacgc
      121 agaacccgca gcggaccata ctgcggggat tcggctccgc cattcttacc ctggtcatcc
      181 tctgtgtgct gacctttgcc ggatccgttg gcgtggcggg ctgggaagcc gttgtgttca
      241 aggccgacgg tacggattcc gattcgccct taccccttgc cttatctcat gttgtttcaa
      301 acaatcatat cctttatcat cttctgatca ctgtgggatt actgggattg gtcgcttcct
      361 ttcacgggat tatcctggcg gccgggagat ccagttttga attcggccgg gtccgttttg
      421 cacccgcctg gctcggaaag atccacgcgc gttttcatac acccgtaaat gccctgctgg
      481 ccaatatggt catcggcatc gttgcattat gcacggggaa aacggcggag atcatcacga
      541 tttccgtttt cggggccctt accttatata ttctatccat gatctcccta ttcaggctga
      601 gaaaaaacga acccgggttg gatcgtcctt ttaaggttcc cggatatccg gcttcgccac
      661 tggtggcgct gatcattgcc gggatctcct ttattgccat gacattctat aattttacat
      721 tagcgatcgt ttactgcgga ctattggccg gatcttatat cctgtttaaa ttattctccc
      781 ggctaaaaag ttcccccgga cagcattaat aaaaatagcg cccatggacc cgaaagaaat
      841 cctgcattat gtaaaagcac atcccgccgg aaaggtgaag atcgctttca gtgatatgga
      901 tggcgtgctg agggggaaat acatctctac ggaaaagttc ctttcggtgg ttgagcacaa
      961 cagcagtttc tgtgatgtga tctttggctg ggacatgggc gacgcggcct atcataatgt
     1021 aacctacacc ggctggcata cgggctatcc cgatgctccc gcccggatcg acctgaacag
     1081 cttccgcaaa attccatggg aagacgggct tccttttttc ctgggtgaaa tgctggaccg
     1141 ggatggaaaa gcggctgata tatgtccccg gcaattgtta aaaaaggttt tgaaagaggc
     1201 gcaggacctg ggcttcactc cttatttctc ccaggaattt gaatggtaca attttgcgga
     1261 aacccctcag agcgcaaccg aaaagcaatt caggaacctg cagcccctga ccaccggaat
     1321 gtttggctat tcgatcttac ggaccagcct gaagaacgat tatttcaccg atcttttcga
     1381 tctgctgaag aaatttgatt tgcccctgga aggccttcat acggaaaccg gccccggcac
     1441 ctacgaagcc gccatcggat atgcttccct gccggaagcg ggcgaccggg ccatcctgtt
     1501 taaaaccgcg gtaaaagaga ttgcctaccg gcatgggatc atggccagct tcatggccaa
     1561 agtaaatgaa aacctgcccg gttgcggagg gcatatccac cagagcctct gggataaaag
     1621 ttccggtaaa aatttatttt atgaagaaaa agatccgttg aagatgagca ggatcatgcg
     1681 gcaatatact gcgggccagc tgtattgtct tcccgatctg tcggccatct atgctcccac
     1741 cattaacagt tataaacggc tggtggaagg aggatgggcg ccgaccaccc tcacctgggg
     1801 cattgacaac cggacagtgg cgctccgtgt gctgaaccac tcagaaaaat cctgccggct
     1861 ggagacccgg gtggtcggag cggatgtaaa tccttacctg gctatggcgg ccgccctcgc
     1921 ttccggtttg tacggaatta aaaaccagat cgatctgagg cagcctccaa ctaacggcaa
     1981 cggatacctg gatactgcca acggacatct tccgcacacc ttagagcagg cgaccgcgcg
     2041 gatgaagcag tcttccgctc ccggagaatt attgggtgaa atctttatca atcattttat
     2101 ccaaacccgg gaatgggaat ggaagcaaca cctgaaagcg gtcactgact gggagctgcg
     2161 gcggtatttt gagatcatct gagacgggcg ccgaacggtc ctggtaaaaa aaatttccac
     2221 atgcgattcg accatattac ccagtttaat tttcccacgc tgatccgctt cggccccgga
     2281 gcgagtaagg agctgccgga ttatctgaaa aaaaacaggc tggaaaggcc gctgctggtc
     2341 acggatccgg tgatcgccgg actgaaattt ttcaaagcga tcgtagcgga tctggaaacc
     2401 aatcacctcg gagtggaagt tttttccggc atccataaaa acccggtgaa gtcggacgtg
     2461 tatgcaggga ccgcaatgta tgatcagacc cgcagggatt cggtcatcgg gatcggcgga
     2521 ggcgccgccc tggatgtggc ccgcgccatc gtgcttcggg taaatcaccg cgaagacctt
     2581 tttaaatacg acgacctcgt tggaggcgat gtgtttgtta ccaatgatgt tccgcatttc
     2641 ataaccattc ccaccacggc aggcacaggc agtgaagtgg gccgcagtgc gatcatcgca
     2701 gacgatgcca cgcatcagaa aaagattctt ttttcaccca aactgctggc gaaagtggtt
     2761 tttgccgatc cggtgctgac cctggacctt cccgctttca ttacggcagc cacgggaatg
     2821 gatgccctga cgcataatat ggaagccttc ctggcaaaaa tggaccatcc catgtgtgaa
     2881 ggaatagcgc tggaagggat gcgcctgatc agccagtcac tggaaaaagc ggtgaaccgt
     2941 cccgacctgg tttccagaag tgaaatgctg ctggcttccc tgatgggtgc ggttgctttt
     3001 cagaaaggac tgggagtagt gcattcgctt gcgcatccgc tttcctccct gctggatacg
     3061 catcatggcc tggccaatgc cgtgaacctt ccttatggta tggaatttaa ccgggaaggg
     3121 tttgaagaaa aattccggcg aatggccgta gcgctggaat tgagcggtga tcgggaaaat
     3181 gaagtggtca cttatatcag cgatctcaat gagaagatcg gtattccgga acggctgggt
     3241 tccatcggcg tgaaacagga acatgttgaa acgctggcag atcttgcatt tgccgatttc
     3301 gcccatccga ataaccccaa gccggtaagc cgggaagatt ttaaaaaatt atatcagcag
     3361 gcattataaa tatgaaaatc attaatcccg ctacggaaga agagattgcc cgcctgccgg
     3421 aggacgatat ggcctccctg aaagaaaaat tccagggg
//
LOCUS       DASYCU010000142         5409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3949414, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000142 DASYCU010000000
VERSION     DASYCU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949414"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1487
                     /locus_tag="VGM24_02825"
     CDS             51..1487
                     /locus_tag="VGM24_02825"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4154323.1"
                     /translation="MENFDLIIVGTGFASSFFLKKYLEKSPASKRVLVLERGILYPYL
                     ERMRAARHEKPTNAFKDFIFNRETYHYDNPDKTWAFDPNFGGSSNCWTGCTPRFMPND
                     FKIKTLYGVGQDWPLGYEEMEPYYTEAEEIMMIGGPAVTPFPKSKAYPLPPQTLSTVD
                     RLIQAKYNQHYISQPTARATVATGKRNGCCTSSVCQLCPANAKFTIENTLNYLYEDPR
                     VVVKYNAVVFNLIVANDKVNGVAYKMDGRDYEASAEVVALGANAIFNAHILLAAGDGN
                     YYTGRGISDQRGTFVYFKYDRLDNLGGSSIISANGYMLYDGVHRRDYAACFIEHFNDP
                     IIRNEAGKWRKISKFKFVFEDLPNDNNRVMLSEDPLMPKVEYHSHDPYVDRGMDKLKE
                     NIDKTFSFLPLESIEMDNYFQKSEFHICSTTRMGNTAKNSVVDKNLVHHQYRNLFVLG
                     SGAFPAISPSNPTLTLSALSLMAADHSF"
     gene            1526..1972
                     /locus_tag="VGM24_02830"
     CDS             1526..1972
                     /locus_tag="VGM24_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154324.1"
                     /translation="MQRKKFIRLTALAATAGIAAGGGYVFFEDFESLARKIILKDTAS
                     LKIDPPEFDKFFKDVKKYRKWDALFPPAHRQLIKWHYYIDNPLFSLPYATSYDTNRSK
                     IVGTFLLSTDFFLNKMDSSKPLRYRALFDPYLYPCSNPFSNLYYPA"
     gene            2340..>5409
                     /locus_tag="VGM24_02835"
     CDS             2340..>5409
                     /locus_tag="VGM24_02835"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HEY4154325.1"
                     /translation="MKAIFTRFFSMAFLLLLAGFFSTSAIAQTGVLNPNDPIVIYNPA
                     SKPVTPPNGTLVKWVKTTRLSWNSTSLKCYYISGIPFRLKFPKSYSTAADGKTYPLYL
                     FFHGIGEKGTIYDNEYSMAHGGDVHTAAVDNGTFDGFLLYPQSTSASGGWNAAQLSVI
                     YGLIMNHLIPEVKVDPNRVIVNGLSGGGAATWEFVEAYPQLVAADIPMSGASSTDVTK
                     VSSLIYNPIWLLTGGLDVSPSPYTAGQVASAAAAAGCDFSWKLYTTLAHNTWDSTWKE
                     PDYFPFMLRAHKANPWARFGRTDFCPGDPINVTLGVTPGFTSYQWRKNGVVIPGAGSN
                     TINVTDLGTYDCRIERGSTWSVWSPIPVVIKTKLPTVTPDIKVLGLASDVIPAPDGST
                     SVNLQVPTGYVNYVWQKLNPTTTLSNTTNILSGATAGGYQVKVTEQYGCSSSFSNTFN
                     VIDANGPNPPSIPTNLQAVAASQTLIKLSWNEDASPANPETQFEIYQSSSSADGYKLI
                     GFSPAHVDSFAISGLNAKTRYYFKIRAINNTAGSPATLPASALTASDNTAPTAPNNLR
                     TGDISANSVELFWDSAFDDVRVTNYDIYVNGKLSYTTGNQNNYTVYNLTTGQIYTFVV
                     KARDLSGNVSLPSNQVVSAAAFNGLTYKYYTFTGTWNNLPDFRTLTPDATGSVPNVDI
                     SERDQDDNFAFLWEGFIQIPRSGNYTFRTNSDDGSKLYLGALNQTTSPYSFGATALVN
                     NDGLHGGQNVNSSVVNLTAGVYPIAVTYYEQGGGQSIAVSWKTPQSGSSFVTIPDSVF
                     AQKVTFPGSAPSVPSKLTATAASAKKINLSWQDNSNNENGFEIYRATSPTGDFAIVGT
                     TGPNQTSFGDSTCSPATTYYYKIKSIGQYGSSAFNNQDSSSLSYNFYANFTASTLATL
                     TTLTPTDNGLSNNFSLGVTTATTNFGLIFDGYINIRTAGTYTFYTSSDDGSSLYIDGT
                     ATSNRVVNNDGAHGTTEKSGTKSLTVGRHKIRVMFFQQGGGEVLTVSYSGP"
BASE COUNT         1439 a         1387 c         1231 g         1352 t
ORIGIN      
        1 gaaaatatat tttataggga aatcgctata aagccagtta agaaaattta atggaaaatt
       61 ttgatctgat cattgttggc accggtttcg cttccagttt ttttttaaag aaatatttag
      121 aaaaatcccc ggcatccaaa cgcgtgctag tcctggaaag aggcattctt tatccctatc
      181 tggaaagaat gcgggcggca cggcacgaaa aacctaccaa tgcctttaag gattttattt
      241 tcaaccggga aacctatcat tacgataacc ccgataagac ctgggctttt gatccgaact
      301 tcgggggcag ctccaactgc tggaccggct gtacgccgcg ctttatgccg aatgatttca
      361 aaataaaaac cttgtacggg gtagggcagg actggcccct gggctatgaa gaaatggagc
      421 cctattatac cgaagcggaa gaaatcatga tgatcggagg tccggccgta actccttttc
      481 ctaagagcaa agcgtatccg ttacctcccc agacactcag tacggtggac cggttgatcc
      541 aggcaaaata taaccagcat tatatcagtc agccaacggc aagggccacc gtggctaccg
      601 gcaaaagaaa cggctgctgt accagctcgg tttgccagct atgccccgcc aacgccaagt
      661 tcaccattga aaataccttg aactacctgt atgaagatcc gagggtggtg gtcaaataca
      721 acgcggttgt ttttaatctg atcgttgcga atgacaaagt gaacggagtg gcctataaaa
      781 tggatggaag ggattatgaa gccagtgccg aggtcgtggc attgggtgcg aatgccattt
      841 ttaatgcgca tattctgctg gcggccgggg acggaaatta ctataccggc cggggaatct
      901 cggatcagcg gggcacgttt gtatatttca aatacgatcg cctggataat ctgggtggaa
      961 gctctattat ctcggccaac ggctatatgc tttatgacgg agtgcacaga agggattatg
     1021 ccgcttgttt tatagagcat ttcaatgatc cgataatccg gaatgaagcg ggcaaatggc
     1081 ggaaaatctc aaagtttaaa tttgtattcg aagatctgcc caacgacaat aaccgggtga
     1141 tgttgagtga agatccgctg atgccaaaag ttgaatatca ttcccatgat ccctatgtgg
     1201 accggggcat ggataagctg aaagaaaata tagataagac cttttccttt ttacccctcg
     1261 aaagcatcga gatggataat tattttcaaa aatccgaatt ccatatctgt tctacaaccc
     1321 gtatgggaaa tacggcgaaa aacagcgtgg tcgataaaaa cctggttcac catcaatacc
     1381 ggaacctgtt tgtattgggc agcggagcgt tcccggcgat ttcgccttcc aatccaaccc
     1441 tgaccctttc ggccttatcg ctgatggcgg cagatcattc tttctaggcc ggagggccgg
     1501 aaatatccgg aaaacagtag aaaacatgca aagaaaaaaa tttatcagat taactgcact
     1561 ggccgccacg gcagggatcg ccgccggggg cggatacgtt ttttttgagg atttcgagag
     1621 cctggcccgg aagatcattt taaaagatac cgcctccctt aaaatcgacc ccccggaatt
     1681 tgataaattc tttaaggacg tgaaaaaata caggaagtgg gacgccctgt ttccgcctgc
     1741 tcaccggcag ttgataaaat ggcattatta cattgacaat ccactattta gcctgcccta
     1801 tgccacgagc tacgatacca acagaagcaa gatcgtgggc actttcctgc tttccaccga
     1861 ttttttcctg aataaaatgg attcatccaa acccctaagg tacagggcct tattcgatcc
     1921 ctatctgtat ccctgctcca atcccttttc caacctctat tatcccgcat agcccggcca
     1981 ccggccggca taagccggaa gcctcattaa caaaataatt tcccccaaaa actgtgaggg
     2041 tttaaaaaaa agatatattt tgcgcttgct ttatacagga ccgacctgat ggcccaaatc
     2101 tcctttttgt aaaaccatcg taatttcctt ctatcccctg aattccgatt gtccttatac
     2161 gattgcagcc tcgcaaaatt ttcattattc tatttcttcc agatcctggc accttacatt
     2221 tttttcttcc agatcctaat gtcaaacttt aatatagatt attaatactg acaatcattt
     2281 ttgccataaa tacgaataac atcaacccac tttttcccat ttaaagaacc ttagattata
     2341 tgaaagcaat ttttacccgt ttcttcagta tggctttctt gctgcttctg gcaggatttt
     2401 tttcaacctc agccattgcc cagaccggcg tactgaaccc aaatgatccc attgtcattt
     2461 acaatccggc aagcaaaccg gtaacgccac caaatggtac cctggtgaaa tgggtaaaaa
     2521 ccacccggct tagttggaac tcaacaagtc tgaaatgcta ttacatcagc ggaattcctt
     2581 tccggctgaa atttccaaaa agttattcca cagctgcgga tggtaaaacc tatccgcttt
     2641 atttgttttt tcacggtatc ggagaaaagg gaaccattta cgacaatgaa tattcaatgg
     2701 cccacggagg cgacgtacat accgctgcgg tggataacgg cacgttcgac gggtttttgc
     2761 tttatcccca atccacttca gcttccggcg gatggaatgc ggcccagctc tccgtgatct
     2821 acggcctgat catgaatcac cttattcccg aggtgaaagt ggatcccaac cgcgtgatcg
     2881 taaacggtct atccggcggg ggagccgcta cctgggagtt tgttgaagcc tacccgcaat
     2941 tagtggctgc cgacattccg atgagcggcg cttcttcaac ggatgttacc aaagtatctt
     3001 ctcttattta caatcccatc tggcttttga caggtggcct tgacgtttca ccatctccct
     3061 atacagccgg tcaggtagcc agtgcggctg ctgctgcagg ttgtgatttc agctggaagc
     3121 tctataccac cctcgctcac aatacctggg actccacctg gaaagaacca gattatttcc
     3181 ccttcatgct ccgggcgcat aaagcgaatc cctgggccag attcggaaga acggattttt
     3241 gtcccggcga cccgatcaat gtcaccctcg gtgttacccc cggattcacc agttatcaat
     3301 ggaggaagaa tggtgtcgta atcccgggag ccgggtccaa taccattaat gttacggatc
     3361 tgggaaccta tgattgccgt attgagcgcg gttcaacctg gtctgtctgg tcaccgattc
     3421 cggtggtcat caagactaaa ctgccaaccg tcacaccgga tatcaaggtc cttggcctgg
     3481 ccagcgatgt gattcccgct cctgacggaa gtacctccgt taaccttcag gtgcctaccg
     3541 gatatgtgaa ttatgtatgg cagaaactga atccgaccac aacgctttct aatactacga
     3601 atatcctctc gggagccacc gcgggcggtt atcaggtaaa agttaccgag caatatggtt
     3661 gttcgagcag cttttccaat acattcaatg taattgatgc caatggaccc aatccgccat
     3721 ccatcccaac caacctgcag gccgtggcgg cttcgcagac cctgatcaag ctcagctgga
     3781 atgaagatgc ttccccggca aatccggaga cccagtttga aatttaccaa tcctcttcga
     3841 gtgcggacgg gtataaactg atcgggttct caccggctca tgtggattct tttgccatat
     3901 ccggactgaa cgctaaaaca agatattatt tcaagatccg ggcgatcaat aacacggccg
     3961 gttctccagc cacgctgccg gcaagcgccc tgaccgcttc cgacaatacc gcgccaacag
     4021 ctccgaataa cctgcgtacc ggcgatattt ccgccaattc cgtggaattg ttctgggatt
     4081 cagcatttga tgatgtccgg gtaacgaatt atgatattta tgttaacggc aagctgagtt
     4141 atactacagg caatcagaat aactatacgg tctataatct gaccaccggc cagatctata
     4201 cctttgtagt gaaggccagg gatctttccg ggaacgtatc ccttccaagt aaccaggtgg
     4261 tcagcgcagc cgcatttaac gggctgacct ataaatatta tacgttcacg ggaacctgga
     4321 acaatttgcc cgatttccgg acccttacgc cggatgcaac cggcagcgtg cccaatgtgg
     4381 atataagcga aagggaccag gatgataatt tcgccttcct ctgggaagga tttatacaga
     4441 tcccgagatc aggcaactat acgttccgga ccaattcgga tgatggaagc aagctctatt
     4501 taggtgccct gaatcagacc acttcccctt attccttcgg cgccacggcg ctggtcaata
     4561 acgatggttt gcacggcgga cagaacgtga acagcagtgt ggttaatctc accgccggtg
     4621 tatatccgat cgcggttact tattatgaac agggcggcgg gcaatcgatc gcggtttcct
     4681 ggaagacgcc acaaagcggc agctcgtttg taaccattcc ggatagtgtt tttgcacaaa
     4741 aagttacttt cccgggatca gcaccgtcag taccttcaaa actgacggca accgctgcct
     4801 ctgcgaaaaa gatcaatctg agctggcagg ataacagcaa caacgaaaac gggtttgaga
     4861 tctaccgggc aaccagtcct accggcgatt ttgcgatcgt cggcacaacc ggtccgaacc
     4921 agaccagctt tggcgattcc acctgcagcc cggcaacgac ctactattat aagataaaat
     4981 caatcggaca gtacggttcg tctgctttca ataaccagga cagcagttcg ctcagctata
     5041 acttctacgc caactttacg gccagtacgc tggctacgct gaccaccctt acgccgaccg
     5101 acaacggcct cagcaataat ttttcgctcg gcgtaacaac ggccacgacc aatttcggac
     5161 tgatattcga cggatatatc aatatcagga ctgccggcac ctataccttc tataccagca
     5221 gtgatgacgg aagttccctg tatattgacg gcactgccac cagcaaccgc gtcgttaaca
     5281 acgacggggc gcatggaaca actgaaaaat caggaaccaa gagccttacg gtgggacgtc
     5341 acaagattcg cgtgatgttc ttccagcagg gaggcggcga agtgctgacc gtatcttatt
     5401 caggtccgg
//
LOCUS       DASYCU010000143         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_616462, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000143 DASYCU010000000
VERSION     DASYCU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616462"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGM24_02840"
     CDS             <1..336
                     /locus_tag="VGM24_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4154326.1"
                     /translation="SLGLFGLSTFMAQQRVKEIGVRKVLGASVSGIVLLLSKDFIKLI
                     LMAVVIAVPLCWWAMNQWLQGFAYRITIGPVVFLEAGMVSLSIALATMAWRSVKAALN
                     NPVNSLRNE"
     gene            complement(401..2788)
                     /locus_tag="VGM24_02845"
     CDS             complement(401..2788)
                     /locus_tag="VGM24_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154327.1"
                     /translation="MLKNFFKTALRNLWKNKAFSFLNIFGLAIGIACAGLIFLWVEDE
                     LSWDQFNVKKDRLYYIRENQKYDAYIATFGSTPTPLAPAMQAEMPGIANTCRIGGWGT
                     TRLIRVGDKSMYSSGSYVDPSLFSMFSLPFVQGNAKTAFAALHSMVITQATARKFFGN
                     EENAVGKTVRVDNRQDYTISGVLKDIPENSTVSFEWVSPFEIWYDENKDWAKDWGNNG
                     LTTFVELKPGVQPATINGLLKGFIQKREAKANVVPFLWSMNDWHLRDEFENGVQTGGG
                     RIEYVHLFSVIAWIVLLIACINFMNLATARSEKRAREVGVKKVLGAGKRSLVFQFIGE
                     ALCLALLSAIGAVLIMALTLPAFNLLVQKNLLISLNNPLHLGVLAGITIICGLVAGSY
                     PSLYLSSFNPVFVLKGLKLKGGSAALIRKGLVVLQFGISIVLIIGIITIYRQVQYVKD
                     RSLGFNKNNLLQVDVNSEMTENYAAVRQDLLASGVVDNLALSNYNTLYGGNNSGGLTW
                     EGKTTGNQVLISMRYVSPGFMNVSGIKILEGRDFVETDSVQSKRMNVMITRSLENQMG
                     KGTAVGKTLHWEGDTSGMVVNVVGVVNDYVYGDMYGKPDPVMFFCLNTKDASQLYVRL
                     KPAEDAEAAMKRIGAVIKKDNPSYPFTYHFVDDQFNRMFLNEELISRLSRVFAALAII
                     ISCLGLFGLAAYTAERRTKEIGVRKVLGASVTHITALLSKDFIRLVLIACLVAFPIAW
                     WMMHRWLQGYKYRIEINWWIFLAAGLSAIAIALLTVSFQSIKAAIANPARNLRSE"
     gene            complement(2968..>3339)
                     /locus_tag="VGM24_02850"
     CDS             complement(2968..>3339)
                     /locus_tag="VGM24_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4154328.1"
                     /translation="TGTNIFVDSTALPADLIRDMEETTRKLHAKPELTAKEKASLKYN
                     DLDLYQPQWKASDLDRITTPTLVIGGDHDLIPVPHTVLIWQHIPDAWLWIVPHSGHGT
                     LVEHRDDFEKNVLAFFRADKK"
BASE COUNT          819 a          888 c          783 g          849 t
ORIGIN      
        1 tctcttggcc tgttcgggct ttccactttc atggcgcaac agcgcgtaaa ggaaatcggc
       61 gtccggaaag tgctgggtgc ttcggtcagc ggaatcgtcc tgctcctgtc gaaggatttt
      121 atcaaactca tactcatggc cgttgtcatt gccgtcccgc tttgctggtg ggccatgaat
      181 caatggcttc agggatttgc ttaccggatc acgatcgggc cggtcgtgtt cctggaagcg
      241 ggaatggtat ccctcagcat tgccctggcc acgatggcgt ggcggtcggt gaaagcggcg
      301 ctgaacaatc cggtcaattc attgaggaat gaatgagagc gcaagcgtat ggttccggat
      361 gaataaaccg gatgcggccg cttcctgaat aatgcaatat ttattcggat ctcaaattcc
      421 gggcaggatt ggcgatggcg gcttttatgg attgaaagct gacggtaagc agggcaatcg
      481 ctattgccga gaggcccgct gccagaaata tccaccagtt aatttcgatc cggtatttat
      541 atccttgaag ccatctgtgc atcatccacc aggcaatggg aaaagcgacc aggcaggcga
      601 tcagtaccag ccgtataaaa tccttcgaca acagggcagt gatatgggta acgcttgcgc
      661 ccagcacttt ccgtacgcct atttctttcg ttctccgctc cgccgtgtat gccgccaatc
      721 caaataaacc caaacaggaa ataataatgg ccagtgcggc aaacaccctg gaaagccggc
      781 ttatcagctc ttcatttaaa aacatccggt taaactggtc atccacaaaa tggtaggtga
      841 agggatagga aggattgtct ttttttatta ccgcccctat ccttttcatg gcggcttccg
      901 catcctcagc cggttttaaa cgcacataca gttgcgatgc gtcctttgtg ttcaggcaaa
      961 aaaacataac cggatccggt ttgccataca tatctccata aacataatcg ttcaccacac
     1021 ccaccacatt tacaaccatc ccgctggtat cgccttccca atgcaacgtt ttacccactg
     1081 cggttccttt acccatctgg ttttccagcg accgggtgat catcacattc attctttttg
     1141 actgaacgct gtccgtttcc acaaaatccc ttccttccag gatctttatg ccggagacat
     1201 tcatgaagcc cggcgacaca taccgcatgg atatcagaac ctggttaccg gtagtcttcc
     1261 cttcccaggt gaggccgccg gaattatttc cgccgtacaa agtattgtag ttggacagcg
     1321 ccaggttatc aaccactccg ctggccagaa gatcctgccg gacagcggca tagttctctg
     1381 tcatctcact gttcacatcc acctgcagca ggttgttctt attgaatccc aggctcctgt
     1441 cttttacata ttgtacctgc cggtagatcg tgatgatgcc gatgatcagt acaatggaaa
     1501 taccgaactg aagcaccacc agtcctttcc ggatcagggc cgcgctaccg ccttttagtt
     1561 ttaatccttt caaaacgaaa accgggttaa aggatgacag gtagagtgaa ggatagcttc
     1621 cggccaccag cccgcagatg atcgttattc ctgccagcac gccaaggtgc agcggattat
     1681 ttaaagagat cagaagattt ttctggacca gcagattgaa tgcaggcaag gtcagcgcca
     1741 tgatcaatac cgctcctatg gcagaaagca gcgccaaaca aagcgcttcg ccgataaatt
     1801 gaaaaacgag actccgcttg cccgcaccca acaccttttt cacaccgact tcccttgccc
     1861 gtttttcgct cctggcagta gccaggttca taaagtttat acaggcgatc agtaaaacga
     1921 tccaggcaat aacagaaaac aggtgcacat actcaatccg tccgccaccc gtttgaacgc
     1981 cattttcaaa ttcgtcccgc aggtgccagt cgttcatgga ccataagaac ggaaccacat
     2041 tggctttcgc ttccctcttc tggataaatc cttttaataa tccattgatc gtcgcgggct
     2101 gaacccccgg tttcagttcc acaaaagtgg tcaggccgtt attgccccaa tcctttgccc
     2161 agtccttgtt ttcatcatac caaatctcaa aaggagatac ccattcaaag cttacggttg
     2221 aattttcggg aatatctttt aaaacaccac taatggtata gtcctgccgg ttatccaccc
     2281 gaaccgtttt tcccaccgca ttttcctcat tgccaaaaaa cttcctggcg gttgcctgcg
     2341 tgatcaccat ggaatgcaat gcagcgaaag cggtcttcgc gttgccctgg acaaacggaa
     2401 gactgaacat gctgaacagg gaaggatcca catagctgcc ggaggaatac atggatttat
     2461 ccccgacccg gataagcctg gtcgttcccc atccgccgat gcgacaggta ttggcaatgc
     2521 ccggcatctc tgcctgcatt gccggcgcca gcggggtggg tgtggaaccg aaagtggcta
     2581 tgtacgcatc gtatttctga ttttcccgga tataatacag gcggtctttt tttacattga
     2641 actgatccca gctgagttca tcttcaaccc ataaaaagat caatccggca caggcgatgc
     2701 cgatggccag gccaaaaata ttcagaaaac tgaaggcctt gtttttccag agattgcgga
     2761 gggcggtttt gaaaaagttc tttagcataa aaaaagataa gttaccgagt gtttccaaac
     2821 gatatgccag accggcatct aattgattta caacaatcta attagagcaa atccaacccg
     2881 ccaatatcca ttttgataca gggcatgtcc ggtattgata cagttcccgg agggaatatt
     2941 tttattaatc cccgcattga tggatgttca ttttttgtct gcccggaaaa acgccaggac
     3001 attcttttcg aaatcgtcgc ggtgttcgac cagcgttccg tggcccgaat ggggcacgat
     3061 ccaaagccag gcatccggaa tatgttgcca gatcagcacg gtgtgcggca ccgggatcag
     3121 gtcgtggtcg ccgccaatca cgagtgtggg tgtcgtgatc cggtcgagat cggaagcttt
     3181 ccattgcggc tgatacagat cgaggtcgtt atatttcagc gatgccttct ctttggccgt
     3241 gagctccggc tttgcgtgca gcttccgggt ggtctcctcc atatcgcgga tcaggtccgc
     3301 gggcaatgcc gtggagtcta cgaaaatatt tgttcccgt
//
LOCUS       DASYCU010000144         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2353383, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000144 DASYCU010000000
VERSION     DASYCU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353383"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1081
                     /locus_tag="VGM24_02855"
     CDS             131..1081
                     /locus_tag="VGM24_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY4154329.1"
                     /translation="MTEITDTIIPHHFAAKRAGPSLLISAGVHGDEFEPMLAAHELIR
                     KAAPSLIAGRLTVVPLVNVSAYDAGSRYGSDGLDLARICPGNITGSISERYAAQISGM
                     IRKADYYIDMHTGGNLFSILPLAGYMLHASGKILKEQRKMAALFNLPIIWGTEDAPNG
                     RTLSVARDAGVASIYVEYGGGGSAKKEIIKAYTEGCLNVLESLQMISPRKKIKSAQKY
                     WVEDYTPDGGYFQSKMRSPAEGIFSPAVKPGAVVKKMQVWGTITDPHSGLKKEILAEA
                     EGLVLSLRMSAPVKLGDSLGGILPILTAGKRIIGKPDKKT"
     gene            1082..1894
                     /locus_tag="VGM24_02860"
     CDS             1082..1894
                     /locus_tag="VGM24_02860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4154330.1"
                     /translation="MKKKVAIITGASRGIGRATTLKFAEGDFNVTLVGRDLKNLREVS
                     DLISREYKADTLVCRGDIGEEEFIREITGKTFEKWGRLDVLVNNAAWRSVETMRTISR
                     ETWEKTLGVCLTAPAFLAKNSADIMERLKIRGTIINVSSIMSGRAGGNSPAYIACKGA
                     MDSLTYELAIAYGRSGIRVLAVNPGHIETDMSRDYVDGKGEDLSDRMINYVVGATPLG
                     RGGSPEEVAEAIYWLSTEKASFITGTTLTIDGGFTHNLNDYSIKKQQFPKEY"
     gene            1921..3003
                     /locus_tag="VGM24_02865"
     CDS             1921..3003
                     /locus_tag="VGM24_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY4154331.1"
                     /translation="MPAIPAEFRLECGEGQTPLLKSRSIGDTLGIPNLYFKLESMNPT
                     GSYKDRFAGVFVSLLQSRRQNFCLATSSGNTGAALAAYCAAADIRCVLAIVDGAPFGK
                     VRQMQLYGAHAFMIKGFGKDTAVTAGVFAILERIAGSMGLPLPISAYRYCPEGMRGVQ
                     TIAYEILEETEGETDHIFSPSGGGGLTLAVARGCIEYSRQYVSRLPKVHCVQPEGNNS
                     IAGPLRGNLDKAKKIDFSSTAISGLQVPNILDGDEVISTCRTLGGNGYIVTDAEVFKW
                     QRLLALREGIFCEPAAAVALAGLAVAVEKKEVFANDKVICLVTGSGFKDMQSVDQHFQ
                     LPGGERIDGERLDKIVTDISNNIFKR"
     gene            3014..>3741
                     /locus_tag="VGM24_02870"
     CDS             3014..>3741
                     /locus_tag="VGM24_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154332.1"
                     /translation="MPDQTDLLRVLVVGCGNMGSSHAQAYHTVGGFEICGLVSKGKSK
                     DVLNKKLGGGYLLFDNYEKALLATKPDAVCISTYPDTHESFAVKALEKGCHVFIEKPI
                     ADSVAGAERVVKAALKTGKKLVVGYILRHHPSWEQFIRLTQELGKPLVMRMNLNQQSQ
                     GDMWTLHRNLMKSLSPIVDCGVHYIDVMCQMTRSKPIAVSAIGARLSNEIPTGNYNYG
                     QLQIRFEDGSVGWYEAGWGPMISET"
BASE COUNT         1035 a          801 c         1045 g          860 t
ORIGIN      
        1 gatggatata aggagtggct ggatgatccc attcatccaa atcatacagg tcatgctgaa
       61 atagccatgc agttcttcag ggccctgggt atttttgatg taaacgctcc ctcctgtggg
      121 ggaaaataat atgactgaga tcactgatac gataataccc catcattttg ccgcgaagcg
      181 tgcgggacct agtttactga tttctgccgg cgtgcacggc gatgaatttg aacccatgct
      241 cgctgcccat gaacttatac gaaaggcggc gccctcgttg attgccggca ggctgaccgt
      301 tgtgccgctg gtaaatgttt cggcttacga tgccggttca cgatatggtt cagatgggtt
      361 ggatctggca agaatctgcc cgggaaatat aacgggcagt atctccgaac ggtacgctgc
      421 gcaaatcagc gggatgatca gaaaagccga ttattatatc gacatgcata ccggcggaaa
      481 tcttttctcc atccttcctc tggcgggtta tatgctgcac gcgtccggga agatattaaa
      541 agagcagcga aaaatggccg cactgtttaa cctgcccata atctggggaa ccgaagatgc
      601 gcccaatggc agaacgctct cggttgcgag agatgcgggc gtagcttcca tttatgtgga
      661 gtatggcgga gggggttcgg caaaaaagga gattataaaa gcttataccg aaggatgcct
      721 gaatgttctt gaaagcctgc aaatgatatc cccccgcaaa aaaataaaat cagcgcagaa
      781 atattgggtg gaagattaca ctccagacgg tgggtatttt cagtcgaaga tgcggtcgcc
      841 tgcggaagga atattttctc ctgcggttaa acccggtgcg gtcgtaaaaa aaatgcaggt
      901 ctggggaacg ataacggatc cgcattcggg tttgaaaaaa gagatcctgg cagaggcgga
      961 gggtctcgtg ctttccctgc gcatgtcggc gcctgtaaaa ctaggcgatt cgcttggcgg
     1021 aatcttaccc atcctgacgg cggggaaaag aattatcggt aagcctgaca aaaaaacata
     1081 aatgaaaaaa aaggtagcaa tcattaccgg agcttcccgc gggatcggcc gggcgacaac
     1141 tttgaaattt gcggaggggg attttaacgt caccctggtg ggcagggatt tgaaaaacct
     1201 ccgcgaagta agtgatctaa tcagcaggga atataaagct gacacgctgg tatgtcgcgg
     1261 cgatatcggc gaagaagaat ttattaggga aattaccgga aagacatttg aaaaatgggg
     1321 aaggctggat gtgctggtca ataatgctgc ctggcgcagc gtggaaacga tgcggacgat
     1381 cagccgggaa acctgggaaa aaacactggg ggtctgttta accgctcctg cttttctggc
     1441 aaaaaacagt gcggatatca tggaacgctt aaaaatccgg ggcaccatca taaatgtttc
     1501 gagcatcatg tccggcaggg caggcggcaa cagcccggcc tatattgcct gcaagggagc
     1561 aatggacagc ctgacttatg aattggctat tgcctacgga agaagcggga tacgtgtgct
     1621 ggctgtaaat cccgggcata ttgaaacaga tatgagccgg gactatgtag atggtaaagg
     1681 agaagatctg agcgaccgga tgatcaatta tgtggtgggc gcaacccctt tgggcagagg
     1741 ggggtcaccg gaggaagttg cagaagccat ttattggctg agtaccgaaa aagcttcatt
     1801 cataaccggc actaccttaa caatagacgg tgggtttacc cataacctga atgactattc
     1861 tataaaaaaa caacaattcc caaaagaata ttgagcagtc tctggaaata caatcatttt
     1921 ttgcccgcaa tacctgctga attccggctt gaatgcggcg aaggacagac gcccttgtta
     1981 aagtcccgtt ctatagggga tacgcttgga atacccaacc tgtatttcaa gctggagagc
     2041 atgaacccga caggttctta taaggatagg tttgcgggcg tattcgtgag tcttttacaa
     2101 agcagacggc agaatttttg tcttgctact tcaagcggaa atacgggcgc ggccctggca
     2161 gcttattgcg cagcggcgga tatccggtgt gtgctggcca ttgtagatgg cgccccattc
     2221 gggaaggtga gacagatgca attatacgga gcacatgctt ttatgattaa aggttttgga
     2281 aaggacactg ccgtcaccgc cggtgtcttt gccattctgg aaaggatagc cggttccatg
     2341 ggactgccat tgcctataag tgcataccgt tactgcccgg agggtatgcg cggcgtccag
     2401 acgatagcct atgagatact cgaagaaacg gaaggagaga cggatcatat attttcgcca
     2461 tcaggtgggg gtggccttac cctggccgtg gcaaggggtt gtatagaata tagccgccag
     2521 tatgtgtccc ggctgccgaa agtgcattgt gtgcaaccgg aaggaaataa cagcatcgcg
     2581 ggtccactca ggggaaatct cgacaaggcg aaaaaaatcg atttttcttc cacggccatc
     2641 agcgggttgc aggtaccgaa tatactggat ggggatgaag taatctccac ctgccgaacc
     2701 ctgggaggaa atggatatat tgtgacagac gcggaagtat ttaaatggca aagattgctc
     2761 gcgctgcggg aaggaatctt ttgcgaaccg gctgccgccg ttgcgctggc cggcctcgcg
     2821 gttgccgtgg aaaaaaagga agtttttgca aacgataaag tgatatgcct ggtaacggga
     2881 agcggattta aagacatgca atcggtcgac cagcattttc agctgcccgg aggggaaagg
     2941 atcgacggcg aacggctgga caaaatcgta acagatataa gtaataatat ttttaaaaga
     3001 taaaaatcat cacatgcctg atcaaacaga cttattgaga gtgctggtgg taggatgcgg
     3061 caatatggga tcctcccatg cacaggccta tcacaccgta ggcggatttg agatttgcgg
     3121 cctcgtttcc aaagggaaaa gcaaggacgt tttaaataag aagctcgggg gaggatatct
     3181 attgttcgac aactatgaga aagcgctgct ggctacaaaa cctgacgccg tttgtatttc
     3241 aacctatccc gacacacacg aatccttcgc tgttaaagcg ctggaaaaag gatgtcatgt
     3301 attcattgaa aaaccaatcg ccgattctgt tgcaggagca gaacgggtgg tgaaagcggc
     3361 attgaaaacc ggtaaaaaac tggtggtggg ttatatttta cgccatcatc cttcctggga
     3421 acagtttatc cgcttaacac aggaattagg caaaccactg gtgatgcgaa tgaatcttaa
     3481 ccagcaaagc cagggggata tgtggacgct ccaccggaat ttgatgaaaa gtttaagtcc
     3541 gatcgtggat tgcggagtgc attatattga tgtcatgtgc cagatgacga ggtccaagcc
     3601 aatcgccgtc agcgccatcg gggcaaggct cagcaatgaa attcccacgg gtaattataa
     3661 ctatggccag ttacagattc gttttgaaga tggctccgtt ggatggtacg aagcgggttg
     3721 gggaccgatg atcagcgaaa c
//
LOCUS       DASYCU010000145         7319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3804224, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000145 DASYCU010000000
VERSION     DASYCU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804224"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="sucC"
                     /locus_tag="VGM24_02875"
     CDS             <1..368
                     /gene="sucC"
                     /locus_tag="VGM24_02875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HEY4154333.1"
                     /translation="GLAMATMDMIKLSGGEPANFLDVGGSANAQTVEAGFRIILKDPK
                     VKAILINIFGGIVRCDRVAQGVIDAYHSIGTIKVPIIVRLQGTNAVEAKKLIEESGLK
                     VQSAILLSEAAALVNKAVA"
     gene            complement(375..830)
                     /locus_tag="VGM24_02880"
     CDS             complement(375..830)
                     /locus_tag="VGM24_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEY4154334.1"
                     /translation="MKKILFLLSLVTAVFQLSAQQKSHNPPHSGRHVKKKSSKPAEKK
                     YPTHYGTASFYANKFEGRRTSNGEIFSQQKFTAASNHIPMNSWVRVTNMRNKRSVILR
                     INDRMHPKNPRLIDLSRSAASRLRYTGRGIVRVKVEVLGKHLPPGIVKD"
     gene            complement(982..2664)
                     /locus_tag="VGM24_02885"
     CDS             complement(982..2664)
                     /locus_tag="VGM24_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY4154335.1"
                     /translation="MQMRQFRISLLIFILLPVFLIQCKNKSNKPPEKDIVVNPARLSE
                     RTTDDIRSTLDYLKKHDGRLNDSVSFSFTSLLDSIYEINNYRPKWFKEDQTLPAGDSL
                     LGFIRNGLLSGLFPNDYHNRMLSFIQRVFQVDSVARKNAAMWARADLLMTDAFFLLAK
                     DLKQGRLPYDSVTLRTDTVLPDHFYTTALDEAMQQGNVDSVLHELEPRSKGYDSLKAC
                     IPAFLAGASFMPYTHLPYPYTDSVRFFALLQKRLEETGDLPEGTEAMDTTAFKRTIRK
                     YQRKYGFKLTGWISDPLIDRLNNTDLEKFKRIAITLDRYKKLPDSLPETYAWVNLPAY
                     RLDIMDHDTLVFSSRVIVGGPQTRTPVLTSEISNFITMPQWTVPYSIIFKEMLPKILK
                     DTLFLSKENLMVVDRNDSVLDPRKINWRRLNKNHFPYQIKQREGDDNSLGVIKFNFRN
                     KYSVYLHDTNVRWMFGKSNRALSHGCVRVKEWEKFADFLIRNDSLKYPADTLKAWVAR
                     QEKHVISGFHKLPLFIRYFSCEGKDGKIRFYEDIYDEDRYLSQKYFANKIIE"
     gene            2769..3461
                     /locus_tag="VGM24_02890"
     CDS             2769..3461
                     /locus_tag="VGM24_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY4154336.1"
                     /translation="MIFLTLESVPVSSQSLPGSAYGLPVVNSISLFEKTCLHHPEKEM
                     MALLPGNGLSLDLRYETKNNFTHQVLYREKPRQTYLRKPAAMALDSVLSDLKRMNLGL
                     KIFDAYRPYFITVKLWEKEPDPRYAADPAKGSGHNRGISVDLTLIDLKTNRPLEMPTG
                     FDNFTDSAHQDFMSLSPRIIGNRNLLKEVMEKHGFIALPTEWWHFSWPHPEDFDVLDL
                     DFDELKRQVDKK"
     gene            complement(3492..4499)
                     /locus_tag="VGM24_02895"
     CDS             complement(3492..4499)
                     /locus_tag="VGM24_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY4154337.1"
                     /translation="MNSFGRIFRITIFGESHGAGVGLVIDGCPAGLPLSAEDFTVDTE
                     RRKGGTQKGTTPRQESDLPIFKSGIFNERTTGAPICILFENNNTRSSDYEKLRAVPRP
                     GHADFVAHRKFGGFEDYRGGGHFSGRLTVALVAGGVIAKKLLKTLQTEVEAKITEIGG
                     SSDIEAGLQKAIDAKDSVGGIVECRVRDLPGSLGEPFFDSVESQIAHIVFAIPAVRGI
                     EFGTGFAAAKMFGTEHNDAILNKEGETVTNHAGGAVGGITNGNELVLRIAIKPTSSTP
                     KEQHTLNWETGKMENFSVKGRHDLCIALRVPVVLEAVTALVLTDFLMLEQRIPRVLSA
                     G"
     gene            complement(4554..5852)
                     /gene="aroA"
                     /locus_tag="VGM24_02900"
     CDS             complement(4554..5852)
                     /gene="aroA"
                     /locus_tag="VGM24_02900"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY4154338.1"
                     /translation="MHVTISPSSIRGTITAPASKSSMQRACAAALISGGECIIKNPGS
                     SNDDNAAIRVIRGLGALVENRSDGSLHILSKGIRPVNDFVNCGESGLGIRMFTPLIAL
                     SDHQMTIEGEGSLLSRPMDFFDEIFPLLRVDFRSNQGKLPLQLKGPLQPANIQTDGSL
                     SSQFLTGLLMAYAAADARDVSIRVANLKSRPYIDLTLKIMRDFGLKVPENRSYESFYF
                     AAEKHVQREALTGYAVEGDWSGGAFLLVAAALAGRCVIRGLDPESTQADKAILQALKE
                     AGAPLQVSGQEIRVGGDPVPALNGFRFDATDCPDLFPPLVALASGCSGSSFIKGVSRL
                     THKESNRALTLQEEFAKLGVDILLNDDIMGIKGVDELRAGTVHSRHDHRIAMATAVAA
                     LRANGPVLIEKADAINKSYPDFFKDLEKLGASVHALDRIY"
     gene            complement(5859..6890)
                     /gene="aroB"
                     /locus_tag="VGM24_02905"
     CDS             complement(5859..6890)
                     /gene="aroB"
                     /locus_tag="VGM24_02905"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY4154339.1"
                     /translation="MQKKTYSFSSARTTCYFEASFAQIGKVVEKDRCVFVTDQHVFDQ
                     HKKYFKGSRVIVIQAGESFKVQQTIDYIIDQLIEFGADRKTTVVGVGGGVVTDISGFA
                     ASVYMRGIPFGFVPSSVLGMVDASIGGKNGIDVGHFKNLVGTIRQPAFLYYDISLLQT
                     LPPEEWVNGFAEVIKHSVIKDAALFKELEKNSLQYYRKNKKALAALIRKNVMIKSAVV
                     RKDEFEEGERKLLNFGHTLGHAVENIYSLPHGHAVAIGIKAACLISEELLDFKETARV
                     TALLEKYGLPTDLHTDPGKIMEIMRMDKKKTRDIMNYVLLEKIGKAVVKPIPMKQLEK
                     LIYSISRAR"
     gene            complement(6907..>7319)
                     /locus_tag="VGM24_02910"
     CDS             complement(6907..>7319)
                     /locus_tag="VGM24_02910"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY4154340.1"
                     /translation="GLIIESHVDPDKAWSDAKQQVTPEKLAELLDGIRWRKEDVASEE
                     YHAALEKLRQQINHLDDELMQIIGERMKVSEKIGLYKKENNITILQTNRWNAILERAF
                     KKGDKLGLSREFITKYFDAVHMESINHQNKVMNS"
BASE COUNT         1757 a         1902 c         1769 g         1891 t
ORIGIN      
        1 ccgggctggc catggcgacc atggacatga tcaaactcag cggcggtgaa cccgcgaatt
       61 tcctggacgt cggcggaagc gccaatgcac aaaccgttga agccggcttt cgcatcatat
      121 taaaagatcc caaagtaaaa gcgatcctca ttaatatatt cggcggaatt gtccgctgcg
      181 accgcgtggc acagggcgtc attgacgcgt atcattccat cgggaccatc aaggtgccga
      241 tcatcgttcg cctccagggc accaatgcgg tagaagcgaa aaagcttatc gaggaaagcg
      301 gactgaaagt gcagtctgcc attttactga gtgaagccgc tgcactggtg aacaaggcgg
      361 tggcttagca aggatcagtc ttttacaatt ccgggaggaa gatgttttcc gagcacctct
      421 acttttaccc gcacaatccc ccttccggta tatcggaggc ggctggccgc ggaacggctc
      481 aggtcaatca ggcgggggtt tttgggatgc atccggtcat tgatgcgcag gatcaccgag
      541 cgtttatttc tcatattggt gacccgcacc cagctgttca tgggaatatg attactggcc
      601 gccgtaaatt tctgctgact gaatatttcc ccgttggatg ttcgccggcc ttcaaatttg
      661 ttcgcataaa aactggccgt gccgtaatgt gtcggatatt ttttttctgc cggttttgaa
      721 gatttttttt tgacgtgccg gccggaatgc ggagggttgt gtgatttttg ctgcgccgat
      781 agctgaaata cagcggtcac caggctgagc aggaaaagga tttttttcat gaggaagttt
      841 taggcttaac ccggaaccta taagacggcg cgcgcacgga ctcctgatcg tagttccggt
      901 tacgggtatt caatcatcaa aaatccggcc ggtttgatgc attcagcacc cggcttaagc
      961 gctccccctc ccccttctgc gttattcaat aattttattg gcaaaatatt tctgactcag
     1021 gtagcggtcc tcgtcataga tatcctcata gaacctgatt ttgccgtctt tcccttcaca
     1081 actgaaatac cggataaaca ggggaagctt gtgaaatccg ctgatcacat gtttttcctg
     1141 ccgggcgacc caggctttca gcgtatctgc gggatattta aggctgtcgt tccggatcag
     1201 gaaatcggcg aatttctccc attcctttac ccggacgcac ccgtggctga gggcgcggtt
     1261 ggatttacca aacatccagc ggacattggt gtcgtgcagg tatacgctgt atttattcct
     1321 gaaattgaat ttgatgaccc cgagggaatt atcatcccct tcccgctgtt ttatctggta
     1381 aggaaaatga tttttattca gccttcgcca gtttattttc cgcggatcga gcacactgtc
     1441 atttctatcg accaccatca ggttttcctt gctgagaaaa agcgtgtcct tcaggatctt
     1501 gggcaacatt tccttaaaaa taatgctgta gggaacggtc cattggggca tggtgataaa
     1561 atttgaaatc tcgctggtca gaacgggcgt tctggtttgc ggaccgccca cgatcacccg
     1621 ggatgaaaaa accagcgtat cgtgatccat aatgtccagg cggtaggcgg gaagatttac
     1681 ccaggcatag gtttcgggaa gggaatccgg aagcttttta taccggtcaa gggtaatagc
     1741 aatccgttta aatttctcca gatcggtatt gttgagccgg tcgatcagcg ggtctgaaat
     1801 ccatcccgtc agcttaaatc catattttcg ctgatacttt ctgatcgttc gcttaaatgc
     1861 cgttgtatcc atggcttcgg ttccttccgg cagatcaccg gtttcttcca gccgcttctg
     1921 aaggagcgcg aaaaaacgca cggaatcggt atacggataa ggaaggtgag tatagggcat
     1981 aaagctggcc ccggccagga atgccgggat acaggctttc aatgaatcat atccctttga
     2041 tctgggttcc aattcatgca gaacggagtc cacattgccc tgctgcatgg cttcatcaag
     2101 agcggttgta taaaaatgat cgggtaatac cgtatccgta cgaagcgtca cactgtcgta
     2161 cgggagccgc ccctgtttga ggtcttttgc cagcagaaaa aaagcatccg tcatcagtaa
     2221 atcggccctg gcccacatgg cagcattctt ccgggcaacg ctgtccacct ggaaaacgcg
     2281 ctgaataaaa gacagcatgc gattgtgata gtcattggga aagagcccgg acaaaaggcc
     2341 gttccggata aaacccaaca gggaatctcc agcaggcaat gtctgatctt ctttaaacca
     2401 tttcggccga tagttattta tttcataaat actatccagc agagaggtaa aggaaaaact
     2461 gaccgaatcg ttcaaccgcc cgtcgtgttt cttcaaataa tccagtgtgc tgcggatatc
     2521 gtcggtggtc ctttccgaaa gcctggccgg attgacaacg atgtccttct ccggcggttt
     2581 attgctctta tttttgcatt gaatcaggaa aacaggtaaa agaatgaata tcaataggga
     2641 aatacgaaat tgtctcatct gcatgaaccg ggattgaata acgaaattag aggaaccaat
     2701 actttaaaga aacaactaat taaatgaaaa acaaaataat gccatcccgg gttttaaagg
     2761 cattttttat gatattccta acgctggaat ccgttcccgt gtcttcccag tcattgcccg
     2821 ggagcgctta cggacttcct gtggtaaaca gcatttctct ttttgagaaa acctgcttac
     2881 atcatccgga aaaagaaatg atggccctgt tgccgggcaa tggtctttcc cttgatctgc
     2941 ggtacgaaac aaaaaataat tttacccacc aggtcctgta cagggaaaag ccccggcaga
     3001 cctatctgcg aaagcccgcg gcaatggcgc tcgacagcgt tctatccgat ttgaaaagga
     3061 tgaacctcgg tcttaaaatt tttgacgctt accggcctta ttttatcacg gttaaactgt
     3121 gggaaaaaga accggatccc cgttatgctg ccgatccggc aaaaggcagc ggccacaacc
     3181 gcggaatctc cgtcgacctg accctgattg acctgaaaac aaataggcct ttggaaatgc
     3241 ctaccgggtt tgacaatttc acggattccg cacaccagga tttcatgtcg ctaagccccc
     3301 ggatcatcgg gaaccgcaat cttttaaaag aggtgatgga gaaacacgga ttcatcgcgc
     3361 ttccgacaga atggtggcat ttcagctggc cgcatccgga agactttgac gtgctggacc
     3421 tggattttga cgagctaaaa aggcaagtgg ataaaaagta aaagtcaggc ggcctgcccg
     3481 tttataccgg atcaaccggc agataacacc ctcgggatcc tttgttcgag catcaggaaa
     3541 tccgtcagga ccagcgctgt tacggcctcc agcacaaccg gcacccgaag ggcaatgcag
     3601 agatcatggc ggccctttac agaaaaattt tccattttcc cggtctccca gttcagcgta
     3661 tgctgctctt tcggcgtgga agaggtgggc ttgatggcga tgcgtaaaac gagctcattg
     3721 ccgttggtaa ttccacccac ggcgccgccg gcatggttgg taacggtttc cccttcttta
     3781 tttaggatcg catcgttgtg ctccgttccg aacatttttg ccgccgcgaa accggttcca
     3841 aattcaattc cccgaacggc aggtatggca aatacgatat gggcgatctg ggattccaca
     3901 ctgtcaaaaa agggctcgcc taaagatccc ggaaggtcgc ggacacggca ttcaacgatg
     3961 ccgccaacgg aatccttggc gtcgatggct ttctgcaaac cagcctctat atcagaggac
     4021 ccgccgatct ccgtaatttt agcttccacc tccgtctgaa gggtctttaa taattttttt
     4081 gcaataactc ctccggccac cagcgcaacc gtcagccttc cgctaaaatg gccgccaccc
     4141 cggtaatctt caaaaccccc gaatttccgg tgcgccacaa aatcggcatg acccggcctc
     4201 ggaacggcac gcagcttttc ataatccgat gaacgggtat tgttattttc aaagagaata
     4261 cagatgggcg cgcccgtagt cctttcattg aaaataccgc ttttgaaaat aggaagatcc
     4321 gattcctggc gcggtgtggt ccctttttgc gtgcctcctt tccttctttc cgtgtcaacg
     4381 gtaaaatctt ctgccgaaag aggcaatccc gcagggcatc cgtctatgac cagccccaca
     4441 ccggcgccgt gcgactcacc gaaaatggtg atcctgaata tgcggccaaa tgaattcata
     4501 taatttccgt tcaattcgaa ttcctgaaga agacttattt atatctatac ccattaataa
     4561 atgcggtcca gggcatggac agaagcgccc agtttttcca gatccttaaa aaaatcagga
     4621 taggatttgt tgatcgcgtc cgctttttca atcagcaccg ggccattggc ccgcaaagca
     4681 gccaccgccg tggccatcgc aatacggtga tcgtggcggg aatggactgt gccggccctc
     4741 agctcatcca ctccttttat gcccatgata tcatcattca ataagatatc cacaccgagt
     4801 ttggcaaact cttcctgcag ggtgagcgcc cggttgctct ctttgtgggt caggcggctt
     4861 actcctttta taaaactcga cccgctgcaa cccgatgcca gggccaccag gggcggaaaa
     4921 aggtccgggc aatccgtggc atcgaaacga aatccgttca gtgcaggcac cggatccccg
     4981 ccaacccgga tttcctgtcc ggatacctgc aggggcgctc ccgcttcttt cagggcctgc
     5041 agaatagcct tatccgcctg cgtggattcc gggtccagcc cgcggatcac acatctgccc
     5101 gcaagggcag cagctaccag cagaaaagcg cctccgctcc agtctccctc caccgcatat
     5161 ccggttagag cttcccgctg aacatgtttt tcagcagcaa aataaaacga ttcatacgac
     5221 cggttctccg ggacctttaa cccgaagtcg cgcataatct ttaacgttag gtcgatatac
     5281 ggccggcttt tcaggttggc cacccggatg gagacgtccc gggcatcggc agcggcataa
     5341 gccatcagta aaccggtcag gaactgcgaa ctgagtgatc cgtcggtttg aatattcgcg
     5401 ggttgcagag ggcccttcaa ttgtaaaggc agtttgccct ggttcgaccg gaagtcgacc
     5461 cggagcaaag ggaaaatttc atcaaaaaaa tccatgggcc ggctaagaag gcttccttcc
     5521 ccttcaatgg tcatctgatg gtcgctgagg gcgatcagcg gggtgaacat ccggatcccg
     5581 agcccgcttt cgccacaatt cacaaaatca tttaccggcc ggatgccctt gctaagaata
     5641 tgcagcgatc cgtcgctccg gttttccacc agcgctccca ggccccggat gacccgtatc
     5701 gccgcgttat catcgttgga agatcccggg ttttttatga tgcattctcc gccggagatc
     5761 agggcagctg cacaagccct ttgcatggag cttttggatg ccggtgcagt aatggttccc
     5821 cggatggaag atggtgaaat ggtaacgtgc atgttaattt atctggctct cgaaatggaa
     5881 taaatcagtt tttccagttg cttcatggga atgggtttga caacggcctt gccgattttc
     5941 tccagcaata cgtaattcat gatatcccgt gtcttctttt tatccatccg catgatctcc
     6001 attatttttc cgggatccgt atgcaggtcg gtgggcagtc cgtatttttc aagcaatgcc
     6061 gtaacccggg cggtttcttt aaaatccagc agttcttcgg agatcaggca ggcggctttg
     6121 attccaatgg caaccgcgtg tccgtgcggc aaggagtaaa tattttccac cgcatggccc
     6181 agggtatgtc cgaagtttaa cagtttccgt tccccttctt caaactcatc cttcctgacc
     6241 accgccgact tgatcattac gtttttgcgg atcagggccg ccagcgcttt tttatttttt
     6301 ctgtagtatt ggagcgaatt tttttccagt tccttaaaaa gggcggcatc cttgatgacc
     6361 gaatgtttga tcacttctgc gaatccgttt acccattctt ccggaggcag tgtctgcagg
     6421 agggaaatat cgtaatagag aaaggcgggt tgccggattg ttcccaccag gtttttaaaa
     6481 tgtcctacgt cgattccatt ttttcctccg atggaggcat ccaccatgcc gagtacggaa
     6541 ctgggaacaa aaccaaaggg tattccgcgc atatagacgg acgcggcaaa accactgata
     6601 tccgtaacca cgcctccgcc gacccccacg acggtcgtct tccggtccgc tccgaactca
     6661 atgagctggt caatgatata gtcgatcgtt tgctggacct taaaggactc tccggcctgg
     6721 atcacgataa cccgggatcc tttaaaatat ttcttatgct ggtcaaaaac atgctggtcg
     6781 gtgacaaaca cgcatcggtc tttctccacc accttaccga tctgggcaaa ggacgcctca
     6841 aaataacagg ttgtccgcgc agaagaaaaa gagtatgttt ttttttgcat ttgcttaatt
     6901 gaatcctcag gaattcatca ctttattctg gtggttgata ctttccatat gtacggcgtc
     6961 aaaatatttg gtaataaatt ccctgctgag ccctaattta tcgcctttct taaaagcccg
     7021 ttccaatatc gcgttccagc gattggtttg caggatcgta atattattct cctttttata
     7081 caggccgatc ttttcggata ctttcatgcg ctcgccgatg atctgcataa gttcatcgtc
     7141 gaggtggttg atctgctgcc ttagcttctc cagcgctgca tggtattcct cggaagccac
     7201 gtcttctttc ctccagcgga tgccgtcgag caattcagcc agcttttcgg gggtaacctg
     7261 ctgtttggca tcgctccagg ctttgtctgg atccacatga ctttcaatga tcagaccgt
//
LOCUS       DASYCU010000146         8436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3804309, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000146 DASYCU010000000
VERSION     DASYCU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804309"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1409
                     /locus_tag="VGM24_02915"
     CDS             18..1409
                     /locus_tag="VGM24_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY4154341.1"
                     /translation="MKRSTTYIQVVLTVALFISGTAAANNIVLNAGFEASPDSPANWT
                     ISGPVAAMEPVTTIDKNNPYRGSFALKMESNNPNAHGRAVQTVPVTGGLTYLFSARFL
                     TKKVSSIDKSVMIKITWYKGTENMGYNYIYDITGDSDNWLLASDKIKAVEGATTAEIS
                     LEFRWGTGTVWWDDISMETCPDLPARKVKVGTVYFRPPGPSVEKNIELMGKLLDEAGT
                     SGCRIVCLPEGWPTSNTGLGMKKIEVNTLEGSASAMMAQKAKQYGMYIVSGLYSWIGD
                     TLSNVAVLYDRQGKIQGVYKKLQLPDNEAEAGAVPGNSFPVFTTDFGKIGMLICWDYA
                     FPEVPRALALNGAEILFCPIAGDIRGTDGYKIISRSRALDNGVYFVNSIYDGHSMIIN
                     PAGDILKETGTQGTLITATIDLNFNPPWVWIGNAGHGIWKGVWRKDRRSDIFGILGNY
                     ETSSGLPAHGLSR"
     gene            complement(1391..1852)
                     /locus_tag="VGM24_02920"
     CDS             complement(1391..1852)
                     /locus_tag="VGM24_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154342.1"
                     /translation="MYQLSKTEFLQQYGRNDSSRALIRFYFAKRYRARNLILLSGAIG
                     IVSGAFIGALALSAGTDSYLWGIFLASLTTLVAEAVITLTLIGGTRLMVMSRKKLLLR
                     LRQYEEEGYIPKNIRNSKFFQSALKGEPDLHRRPARNTEPHKKLNYYLDNP"
     gene            2301..3353
                     /gene="glk"
                     /locus_tag="VGM24_02925"
     CDS             2301..3353
                     /gene="glk"
                     /locus_tag="VGM24_02925"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY4154343.1"
                     /translation="MADFARISLAFPGRRLLGDKKVCVLAGDVGGTKANLAIYEATAA
                     EISLVKTSTYHSGQYPSVIKIIQQFFSENPGYKPDRISLGVAGPVFEGHAEITNLPWT
                     IDKKEITAATGIQRITLINDLEATAYGLAGMEEKDFMVLHQGEPGIRGNMAVLAPGTG
                     LGEAGLFWDEEVHHPFATEGGHSDFSCRSADDMDLHNFLLKKYEVVSWESVIAGPAIH
                     DIYNFLIQEKKRPESNAVAEAFKKDDPSAVVSEMAVAKSDPVCMEAMKFFICYLARES
                     CNLILKMKATGGLFLAGGIPPKIVPLLKEPDFYTHLLECDRMQDLVKKVPVKVLLNDK
                     AAMIGAGWYGAYFLSD"
     gene            3390..4715
                     /locus_tag="VGM24_02930"
     CDS             3390..4715
                     /locus_tag="VGM24_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154344.1"
                     /translation="MDSRRSFIKQAGLLAGGSLLASSLQQSAFASAGRKMAASDQINI
                     GAIGINGMGWADLTAALKQPGVNVVALCDIDKNVLDKRMKDLAAMNVDTSKIKTHGHY
                     REVLDNKDVDAVLIGTPDHWHALIMMEACAAGKDVYVEKPVGNSIGECRAMVAAQKKY
                     NRVVQGGQWKRSQQHFKDAIYFVHSGQLGNIRTVKVWCYQDWMKPGPVVPDTAPPAGV
                     DYKMWLGPAPTRTFNSSRFHFNFRWFWDYAGGLMTDWGVHLIDYALLGMKAGTPKTIA
                     ALGGDFAYPDLYQETPDTLTALYEFDHFNMVWDHAMGIGNGSYGRDHGIAFIGNNATL
                     VLSRGGWEVIDENMNKNKIGRALAKPVDNGVENHWKNFIEVVRSRKMENLNCSIEAGA
                     LVATVAQLGNISLRSGKRLVWQNDRFTDDAVNKEYLMKTYHNGYTLPVV"
     gene            4827..6803
                     /locus_tag="VGM24_02935"
     CDS             4827..6803
                     /locus_tag="VGM24_02935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY4154345.1"
                     /translation="MKNPLLVHNNQPIAFNKVDAGVIRDAVSGVIRLSDQRIRKILRL
                     PLSGTGPGSVLAAYDELIYDLNDLGVKLGLIAQTYPSDSSRDAANNGSQALSEYQTRL
                     LLNEELYKKLKSYESATAGSMKPNQKKFVREQVLIFENNGMKLDSAGRKELQEISDRI
                     TALGLQFDKNLAVSRDSLLFDDTELKGVPDNVKEAWKTPSGKFVVYINTPNAENILMH
                     AEEDNTRRQMYIKYNNRAYPENSKVLDSLFYYRQKYADRLGYKSYAEYALVDKMAANP
                     QNVWNFENNLKEKLAPQVTADLNELRQIKHGLHPELPDTIFAWDIHYYKNILLDTKYK
                     LNTDEVKEYFEMNNTIRGMFEVYRRLLGVTVKETSGIPTWYSKVKTFEMYKEGKKIGS
                     FYFDLYPRENKYTHFACFYISQDRVAGGKETLPVAALICNFPEAAAGKPTLVNHADVI
                     VLFHEFGHLVHAMVVRSDIASQPLTIKPDFVEAPSQFLENFCWEYPSLKLFAKHYKTG
                     AVLPEALFEKLKSTQHVLDAMYYTQQLYYGIIDFTFHDKYDSIKGRDINQVSADLHPM
                     VQYPFVEGTHFIASFGHLNGYAANYYGYLWSRVFAQDIFSVFQKNGIMDPKTGTRYRK
                     DILEVAGSEEEMDMLRHFLGREPNSDAFMKSIGL"
     gene            complement(6831..7562)
                     /locus_tag="VGM24_02940"
     CDS             complement(6831..7562)
                     /locus_tag="VGM24_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY4154346.1"
                     /translation="MKKILVIAFLPLALLLLLSAAPPVEKTPSVLIFSKTNGFRHSSI
                     PAGIAAIKKLGEANGFHVDATEDSLAFNKKNLKKYAAIIFLSPTGKVFGPEQENALQY
                     YIEHGGGYDGIHAATDCEYNWPWYNKLAGAWFKRHPQQQEAKLIVVNKNHPSTAGLPD
                     TWVRKDEWYDFKDLNPDVTVLIRIDETSYKGGEMGSNHPMAWYHSFDGGRAFYTELGH
                     TDESYSDPLYLKHILGGIEYAMGKK"
     gene            complement(7568..8281)
                     /locus_tag="VGM24_02945"
     CDS             complement(7568..8281)
                     /locus_tag="VGM24_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY4154347.1"
                     /translation="MMVKQTGIALFSMILLSTGAMAQAKNNTLTAPEKSEGWQLLFDG
                     SSTKGWHNYNKQAIGPEWKADDGSLHLDASHKSGSDIVSTDKFENFDLKLQWKISKGG
                     NSGILFLVNESPKYEESYFTGPEMQVLDNDGHPDGKIHKHRAGDLYDLIASSTEPVHA
                     VGDWNDVEIKLNHGKLDFYLNGVHTVSTTMWDANWDNLVKGSKFAKMTGFAKSKSGHI
                     DLQYHGFDVWFRNIKIKKL"
BASE COUNT         2094 a         2004 c         2221 g         2117 t
ORIGIN      
        1 attaaagacc atataaaatg aaaagatcaa ccacttatat ccaggttgtt ttaactgtcg
       61 ctctgtttat ttcgggaaca gcagcagcaa acaatattgt attgaacgcc ggttttgagg
      121 catccccgga ttcaccggca aattggacga tcagcgggcc ggttgcagcc atggagccgg
      181 tcactacaat tgataaaaac aacccctacc ggggctcgtt cgcactcaaa atggaaagca
      241 ataatcccaa tgcgcacggc agggcggtgc agactgtgcc cgttaccggc gggcttacct
      301 atcttttcag cgcaaggttt ctaactaaaa aagtaagctc cattgacaaa tcggtaatga
      361 ttaaaattac ctggtacaag ggcacagaaa atatgggcta caactatatc tatgatatta
      421 ccggtgattc cgataactgg cttctggcct cggataaaat aaaggccgta gaaggagcaa
      481 cgaccgccga aatttccctt gaattccggt ggggcacagg aaccgtctgg tgggatgata
      541 tttccatgga aacctgtccc gatctgcctg cgcgcaaggt aaaagtggga acggtttatt
      601 tcaggcctcc cggtccttca gtggaaaaaa atattgagtt aatgggcaaa ctgcttgatg
      661 aagccggcac ttccggctgc cggattgttt gcctcccgga aggttggcca acctcgaaca
      721 ccggtctcgg tatgaagaaa attgaggtga acacattgga aggaagcgcc tccgccatga
      781 tggctcaaaa agcaaaacaa tacggtatgt atattgtttc cggcttatac tcctggatag
      841 gcgatacgct gagcaatgtg gctgtattgt atgaccgcca gggtaaaata cagggtgttt
      901 ataaaaagct gcaattgccc gataacgaag cagaagcggg tgcggttccg gggaattcat
      961 ttccggtatt taccactgat ttcggtaaaa tagggatgct tatttgctgg gactatgctt
     1021 ttccggaagt gcccagggct ttagccttaa atggcgcaga aattttattc tgccctattg
     1081 cgggtgatat ccggggtaca gatggttata aaattatttc aagaagccgg gccctggata
     1141 acggtgttta ttttgtgaac tccatttacg acggccatag tatgatcatc aatccggcag
     1201 gagatattct taaggaaacc ggtacgcagg ggacgcttat caccgccacc atcgatttaa
     1261 atttcaaccc gccctgggtc tggataggaa atgccggaca cggcatatgg aaaggcgtgt
     1321 ggagaaaaga tcgccggtcg gatatatttg gaattctggg gaactacgag acttcttcgg
     1381 ggttgccggc tcatggattg tccaggtaat agttcagctt cttatgcggc tcggtattcc
     1441 gggccgggcg gcggtgaagg tccggttcgc ccttcaaagc ggactggaaa aatttactgt
     1501 tcctgatatt ttttggaata tatccttctt cctcatattg ccttaaccgg agcaggagtt
     1561 ttttccgcga catgaccatc aaccgggtcc ctccgataag tgtcagcgta ataacggctt
     1621 ccgcgacaag cgtggtaagg ctggccaaaa agattcccca aaggtaggag tcggtgccgg
     1681 cggacagggc cagtgcgccg ataaaggctc cggatacaat gccaatagcg ccggaaagca
     1741 ggatcaggtt tctcgcccgg tatctttttg cgaaataaaa gcggatgagt gcccgggagc
     1801 tgtcgttcct gccgtattgc tgcaggaatt ccgtcttgct gagctggtac attgaggccg
     1861 ggacagagtc acttattttt aatggggcgg agccaaagct tgctgaccgg aaaagcagga
     1921 aacaacagaa gcttagtatg aagattcgca tggggtcaat ttggattgag atgcaacgga
     1981 attcggatcc cgccgctggg ttcaatccaa acagtaaact aatcctgttt tacgccgcag
     2041 acaaaattcc cggcttaaag ttttcttaag taaaataatt tgacctgcct tccgttattg
     2101 ataaagtgat tatcataact gaagttttct cacggccggt gtgtctacac tggctttttt
     2161 ttatctcttt cacgtagatt tccggttctc tttcaggtaa aattaccttt cccatttttg
     2221 gaaaatccgg cggcgcacgg ccccggtctc aaggtttttt cgcttcaatc ctttacattc
     2281 gattttcaac cgtcttgctc atggccgatt ttgccaggat ttcactcgcc tttcccggtc
     2341 gtcgtttatt aggggataaa aaagtatgcg ttctcgcggg agatgtgggt ggcaccaagg
     2401 caaacctcgc tatctatgaa gcaactgccg cagagatttc cctggttaaa acctccacct
     2461 atcattccgg gcaataccca tccgtcataa aaataattca gcagttcttc agcgaaaatc
     2521 ccggctacaa gccggaccgg atttcgctgg gcgtggccgg gccggtgttc gaagggcatg
     2581 cggagatcac caatcttccc tggacgatcg acaaaaaaga aattaccgcg gccacgggaa
     2641 tacaaaggat caccctgatc aacgacctgg aggcaacggc atacggcctg gcgggaatgg
     2701 aagaaaaaga ttttatggtt ttgcaccagg gggagccggg catccgcggc aatatggccg
     2761 ttctggcgcc gggaaccggt ttgggcgaag cgggcctgtt ctgggatgag gaagtgcatc
     2821 atccgtttgc aaccgaagga ggccactcag atttttcctg cagatcggcg gatgatatgg
     2881 atctccataa ttttctttta aaaaaatatg aggtggtgag ctgggaaagc gtgattgccg
     2941 gcccggccat tcacgatatc tataattttt taattcagga aaaaaaacgc ccggaaagca
     3001 atgccgtcgc agaggcgttc aagaaggatg atccttccgc cgtggtcagc gagatggccg
     3061 ttgccaaatc cgatccggtc tgtatggagg cgatgaaatt tttcatctgt tacctcgccc
     3121 gggaatcctg taatctgatc ttaaaaatga aggcgaccgg cggactcttt ctggccggag
     3181 gtattccgcc gaaaatcgtt cctttgttaa aggagccgga tttttatacc cacctgctgg
     3241 aatgcgaccg catgcaggac ctggttaaaa aagttccggt aaaggttttg ctgaacgaca
     3301 aggcggcgat gatcggtgcg ggctggtatg gcgcttactt tctttccgac tgaactgatt
     3361 ttattcttca tcctaataat tattgtcaaa tggattcacg tcgttctttt atcaaacagg
     3421 ccggcctgct cgccggcggc tctctgctcg cttcctctct tcagcagtcc gcgttcgctt
     3481 cggccggaag gaaaatggcg gccagcgatc agatcaatat cggcgctatc ggtataaacg
     3541 gaatgggatg ggccgatctc actgcggcat tgaaacagcc cggcgtgaac gtggtggcct
     3601 tatgcgacat agacaagaac gtgctggata aacggatgaa ggacctggcg gcgatgaacg
     3661 tcgatacctc caaaataaaa acgcatgggc attaccgtga ggtgctggac aacaaggatg
     3721 tggatgccgt gctgatcggg acgcccgatc actggcatgc cctgatcatg atggaagcct
     3781 gcgccgccgg caaagatgtt tacgtggaga agccggttgg aaattccatc ggggaatgcc
     3841 gcgccatggt ggccgcgcag aaaaaatata accgggtggt ccagggagga caatggaagc
     3901 gcagccagca gcattttaaa gatgccatct attttgttca ttccgggcaa ctgggaaata
     3961 ttcgtaccgt aaaggtctgg tgttaccagg actggatgaa acccggtccg gtcgttccgg
     4021 ataccgctcc tcccgcaggc gttgattata aaatgtggct gggaccagcg cccacgcgta
     4081 ccttcaactc aagccgtttc cattttaact ttcgctggtt ctgggattat gccggcggtt
     4141 taatgaccga ctggggggtg catctcatcg actatgccct gctgggaatg aaagccggta
     4201 cgccgaagac cattgccgcc ctgggcggtg attttgccta tcccgacctt taccaggaaa
     4261 cgccggatac cttaaccgcc ttatacgaat tcgatcattt taacatggta tgggatcatg
     4321 ccatgggcat cggcaacggt tcatacggaa gagatcacgg gatcgccttt atcggaaaca
     4381 acgccacgct ggtattgagc cggggaggat gggaagtgat cgatgaaaac atgaacaaaa
     4441 ataaaattgg ccgggcgctt gccaaaccgg tggacaatgg cgtggagaac cactggaaga
     4501 attttatcga agtggtccgc tcccgcaaaa tggagaacct gaattgctcc attgaggcag
     4561 gagccctggt ggctacggtt gcgcaactcg ggaatatcag cctgcgcagc gggaaaagac
     4621 tggtctggca gaatgaccgg tttacagatg atgcggtgaa taaggaatac ctgatgaaga
     4681 cctaccataa cgggtatacg cttcccgtgg tttaagcaaa tattcttagt ttgcagggat
     4741 aaactccttt tatgcaccgc cagaacctac tgctaaccgt tgtatttttt ctgatcgccg
     4801 ggatttcatt tgcacaacct gttgcaatga aaaatcccct cctggttcac aacaaccaac
     4861 ccattgcatt caacaaggtt gatgccggag tgatccgcga cgccgtttcc ggggtcatcc
     4921 gtttatcgga tcaacggatc cggaaaatcc tccgcctgcc gctatccgga acaggtccgg
     4981 gaagcgtgct ggccgcctac gatgaactga tctatgatct gaatgacctg ggtgtcaagt
     5041 taggcctgat cgcccaaacc tatcccagtg acagttccag ggatgcggcc aataatggct
     5101 cccaggcttt atcggagtac cagacccgcc ttttgctgaa tgaggaattg tataaaaagt
     5161 tgaagtcgta cgaatccgcc accgcgggca gcatgaaacc caaccagaaa aaatttgtcc
     5221 gtgaacaggt cctgatcttt gagaataacg gaatgaaact ggattctgcg ggacgtaagg
     5281 aactgcagga gatcagcgac aggatcaccg cactgggttt gcagttcgat aagaatttag
     5341 cggtgtcccg ggacagcctc ctgtttgatg atacggaact gaaaggtgtt ccggataatg
     5401 tgaaggaagc ctggaaaacg ccttccggga aattcgtcgt atatatcaac acgccgaatg
     5461 cagagaatat cctgatgcat gcagaggagg ataacacccg ccggcagatg tatataaaat
     5521 ataataaccg ggcgtatccg gagaattcaa aggtgcttga cagccttttt tattaccggc
     5581 agaaatatgc ggaccggctg gggtataaaa gttatgcaga atatgcgttg gtggacaaga
     5641 tggccgccaa tccgcaaaac gtgtggaatt ttgaaaataa cctgaaggaa aaactggctc
     5701 cgcaggttac tgccgatctg aatgaactcc ggcagataaa acacgggctc catccggagc
     5761 ttcccgatac catattcgcc tgggatatcc attattacaa gaatattctc ctggatacaa
     5821 aatacaagct gaacaccgat gaggtcaagg aatatttcga gatgaataat accatccggg
     5881 gaatgttcga ggtgtatcgc cggttgctgg gcgtaacggt taaggaaacc agcggtattc
     5941 ccacctggta ttccaaagtg aagaccttcg aaatgtacaa ggaaggtaaa aagatcggca
     6001 gtttttattt cgatctgtat ccccgggaga ataagtacac ccattttgcc tgcttttaca
     6061 tcagccagga ccgggtagcc ggagggaaag agacccttcc cgtggcggcg ctcatctgta
     6121 atttcccgga agccgctgcc gggaagccta ccctggtaaa ccatgctgat gtcattgttc
     6181 tttttcacga gttcggccat ctcgtacacg ctatggtcgt tcgttccgat atcgcatccc
     6241 agccgctgac cattaaaccc gatttcgtgg aagcgccatc gcagttcctg gagaactttt
     6301 gctgggaata tccttccctg aaactctttg caaagcatta taagaccggg gcggtgcttc
     6361 cggaagcgct gtttgagaag ctgaagtcaa cccagcatgt gctggatgcc atgtattata
     6421 cccagcagct ttattatgga atcatcgact ttacctttca tgataaatac gatagtatta
     6481 agggccggga cataaaccag gtttcggccg acctgcatcc catggtgcaa tatccttttg
     6541 tggaaggcac ccatttcatc gcttctttcg gtcacctgaa cggatacgcg gccaactact
     6601 atggttatct ctggtcccgc gtttttgcgc aggatatatt ctctgttttt caaaagaacg
     6661 ggatcatgga tcccaaaaca gggacccggt accgtaaaga tatcctggaa gtggccggga
     6721 gtgaggaaga aatggacatg ctccgccatt tcctcggaag agagccgaat tcggatgctt
     6781 ttatgaagtc gatcgggctt tgagatgatg gaggctgagc agtgacggcg tcacttcttt
     6841 cccatcgcat attcaattcc cccgagaata tgtttcaggt acagcggatc ggaataggat
     6901 tcgtccgtat gccccaactc ggtataaaag gcgcgtcccc cgtcgaagct gtgataccag
     6961 gccatgggat ggttgctgcc catttccccg cccttatagg aggtctcgtc gatccggatg
     7021 agtacggtca cgtccgggtt gaggtctttg aaatcgtacc actcgtcttt tcgtacccag
     7081 gtgtccggta gtccggccgt ggacggatga ttcttgttta cgacgatgag tttggcttcc
     7141 tgctgttgcg ggtggcgttt gaaccaggcg ccggccagct tgttgtacca gggccagttg
     7201 tattcgcagt cggtggctgc atgaatgcca tcataaccgc cgccgtgctc tatataatat
     7261 tgcaaggcgt tttcctgttc gggcccgaat actttgccgg tcgggcttaa aaagatgatc
     7321 gccgcatatt tttttaaatt cttcttattg aaggccaggg aatcctcggt ggcatccaca
     7381 tggaaaccat tggcctcgcc cagttttttg atcgctgcaa taccggcggg aatgctggaa
     7441 tgccggaatc cgttggtttt agaaaaaata agtacggacg gcgtcttctc tacgggaggc
     7501 gctgcagata acagcaggag cagggcaagc ggcaggaagg caatgaccag gatttttttc
     7561 atgacaatta taatttttta atcttgatat tccggaacca gacatcaaaa ccgtgatact
     7621 gcagatcgat atgcccggat ttggatttcg caaagccggt cattttcgca aatttgcttc
     7681 ctttgaccag gttatcccag tttgcgtccc acatcgtggt ggaaacggtg tgcacgccgt
     7741 tgagatagaa gtccagttta ccgtgattga gttttatttc cacgtcgttc cagtccccga
     7801 ccgcgtgcac gggttcggtg gaagaagcga tcaggtcgta aagatctccg gcccggtgtt
     7861 tatgaatttt gccatccgga tgcccgtcat tatccaatac ctgcatttcg gggccggtaa
     7921 aataggattc ttcgtatttg ggggattcat tcaccaggaa aaggatcccg ctgtttcctc
     7981 ctttggaaat tttccactgt agcttcagat cgaaattttc aaacttgtcc gtggaaacga
     8041 tatccgatcc cgacttatgg gaagcatcca ggtgcagact gccatcgtct gccttccatt
     8101 ccgggccgat ggcctgcttg ttgtagttgt gccagccctt cgtgctgctg ccgtcaaaaa
     8161 gcagttgcca tccttcgctt ttctccgggg cggtcagggt attgtttttt gcctgcgcca
     8221 tcgctccggt ggagagcagg atcatggaga acaaggcgat gcctgtttgc ttaaccatca
     8281 tggactgtta ttttttcagt gagagaatat aagtgaccat ttcttttaca tcgccttcgg
     8341 agagagcggg atggggcgtc atgggtactg tgccccagac accggttcct ccgttgatga
     8401 tcttatgaac gagccggttt acggtggtat cggcag
//
LOCUS       DASYCU010000147         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5111141, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000147 DASYCU010000000
VERSION     DASYCU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5111141"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGM24_02950"
     CDS             complement(<1..365)
                     /locus_tag="VGM24_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEY4154348.1"
                     /translation="MRRTPALLILPLFFALSGRAQIDAGLFAYPDVSKTQIVFSYAND
                     LWIVPKAGGLADKISSPPGVESFPKFSPDGNYIAFTGNYDGNKDVYTIPSTGGVPLRI
                     TEHGYADRVVDWTPDGKSV"
     gene            complement(553..1434)
                     /locus_tag="VGM24_02955"
     CDS             complement(553..1434)
                     /locus_tag="VGM24_02955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY4154349.1"
                     /translation="MTRRETLKQIIHFPLTRIIIGLVVVGGLVALVETTRNFFADKIT
                     LSIETQNWIVAVADVAIALSSYILLYRFYEKRQVRELSAAGFGGNALAGFLSGLILQS
                     LVIFVIYLAGGYSILRVNPVSFLIPGFIAAITAGFVAEILLRGILFRLMEEKLGTVIT
                     LIFFAVLFAILHAGNGATFFSVLSKASQAGILFSAVYVFSRSLWFPIFLHFAWDLAEP
                     GIYGALNPGISIDKYLFTSRISGSAWLTGGEAGPGGSVQTSVFCLIAALLFLWGAKRK
                     NNFISPYWKRRRSEQVS"
BASE COUNT          463 a          405 c          362 g          372 t
ORIGIN      
        1 aaaacgcttt tcccgtccgg ggtccagtcc actacccggt cggcataccc gtgttccgta
       61 atgcgcagcg gcactccgcc ggtggaagga atggtataaa cgtctttgtt cccatcgtaa
      121 tttccggtaa aagcaatgta attcccatcc ggtgaaaact tgggaaaact ctccacgccc
      181 ggcggcgaac tgattttgtc ggcgagaccg cccgctttgg gaacgatcca gagatcattg
      241 gcataggaaa acacaatttg tgtttttgat acatccggat aggcaaataa tccggcatct
      301 atctgtgccc gtcccgaaag agcaaaaaat aacgggagga tgagtaaggc aggtgttctt
      361 ctcatttgat gcagttaaaa tttggatatt gaaggtaggg aaattccgga cttaggaacc
      421 gtacccgatg accgctggtg gaatcatttg accattcatt atctattgcg ggtccctcag
      481 gaaaaagatc cggcccccgg aatccatcct aaaagtatac agcaggcaat taaaatccgg
      541 gggaatacgt gctcagctta cctgttcgct ccggcgcctt ttccaatagg gacttataaa
      601 attattcttt ctttttgccc cccataaaaa cagcagcgca gcgatcagac aaaacacgct
      661 ggtctgtaca gaacctccgg ggccggcttc tccgccggtt aaccaagccg aaccggaaat
      721 tctactggta aataagtatt tgtcaatcga aatacccgga ttaagcgcgc cgtaaattcc
      781 gggttcagcc agatcccagg caaaatgcag gaatatggga aaccataaac tccgggaaaa
      841 aacataaacc gcggaaaata atatacctgc ctggcttgcc ttggaaagaa cggaaaaaaa
      901 agttgctccg ttaccggcgt gtaaaatggc aaacagaacg gcaaaaaaga tcagggtaat
      961 taccgttccc aacttttcct ccatcaaacg aaacaggatt ccccgcaaga gtatttcagc
     1021 aacaaaaccc gctgttatgg cggcgataaa tcccggaatc aaaaaagaaa ccgggttgac
     1081 gcgaagaatg gaataaccgc cggccaggta aataacaaaa atcaccagcg attgtaagat
     1141 caacccgctc aggaacccgg caagtgcatt tcctccaaac cccgccgcac ttaattcccg
     1201 cacctgcctt ttttcataaa acctgtacag gaggatatag ctggacaaag cgatcgcaac
     1261 gtctgccact gcaacaatcc agttttgcgt ttcaatgctc aacgttattt tgtcggcaaa
     1321 gaagttcctg gttgtttcta cgagggccac cagtccgccc accacaacaa gaccgatgat
     1381 gatcctggtt aaggggaaat gaataatttg ttttaaggtt tcgcgacggg tcattgggca
     1441 gctgtccggt catatttttc agctcatcgg ctgaaaccgg ataccctgcg aatataatca
     1501 agctttccct ggctattcaa tcagaaatga tttccgtaag cggatatctt ctgaagagct
     1561 tcctatcatc acttcgaatt cgccgggctc aacggtccag tg
//
LOCUS       DASYCU010000148         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4962977, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000148 DASYCU010000000
VERSION     DASYCU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4962977"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1069
                     /locus_tag="VGM24_02960"
     CDS             113..1069
                     /locus_tag="VGM24_02960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4154350.1"
                     /translation="MGEAVFISICIPAYNRPAKLKRLLDSIQAQSYRHFEVVITDDSP
                     ENEVNDLVHHHPLMPSIRYFKNHPVLGTPENWNEGIRRAGGDWIKLMHDDDWFRDAQA
                     LQVFADGVKKHPDGNFFFSGYRNVFEGKNKTQEMSLSRPWERLLNSNPENLLSKNVIG
                     PPSVVLHKKIPELLYDRNLKWLVDIDFYIRFIRSHPMPVFLPEQLIEVGISEDQVTQS
                     CFRNPLVEIPEHLYLLNKMGTRALKNIILYDAYWRFIRNLNLRSTAEIRNAGYAEPVP
                     RIIRSMIGFQAFFPAAVLKTGIISKGLMALHFIFQYPKLARG"
     gene            1062..2069
                     /locus_tag="VGM24_02965"
     CDS             1062..2069
                     /locus_tag="VGM24_02965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4154351.1"
                     /translation="MVNLSIIIINYKSLPLIRNCLHSIYAARPVLDFEIIIVNNDPLQ
                     AEDPQITQTYPELQWISMGYNSGFARANNEGIKRAAGEVVLLLNPDTLIPETALDRCY
                     RNLVQSEYIACGVQLLNEDSTPQISGNYFVKGGLNYLLPLPYAGKFIKWLAGLFSLKA
                     PHVALASSVTEVDWINGAFLMVKKEGIQKAGMMDEDFFLYAEETEWCARLKKVGRLCL
                     FGSITVFHLQGVSANEAFSSSGKGYYNLFDRKGLQIMLSNLVRIRKQFGKFWFLCVLA
                     IYTLDWPVFLIGILLSFAYPEPDRTYSFHQFRGFTKNLIFLWSKTPVILLNRPYFYKV
                     L"
     gene            2162..>2494
                     /locus_tag="VGM24_02970"
     CDS             2162..>2494
                     /locus_tag="VGM24_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY4154352.1"
                     /translation="MQFKYGTDQLTLLKAIQLAEGRIKGILPDETRENVRKSQLQVAQ
                     IVSRNKTVYGINTGFGALANTKISEDDTRVLQQKILQSHSVGVGKPIDPFLVRLMMIT
                     KVNALCKGF"
BASE COUNT          652 a          594 c          578 g          670 t
ORIGIN      
        1 aataagcata atctgaagtg ttgcattatt atcaacaatt ttcgtgctat attctggtaa
       61 aggaatatta atatgcagat gaatttatcc acactaccgt tatttttgcc tgatgggaga
      121 ggctgttttt atttccattt gtattccggc ctataaccgg ccggcaaaac tgaagcggct
      181 gctggactcc atccaggccc agtcttaccg gcatttcgag gtcgtgatca ccgacgacag
      241 cccggagaac gaagtgaatg acctggtcca tcatcatccc cttatgccct ccatccggta
      301 ttttaagaac caccccgtac tggggacgcc ggaaaactgg aatgaaggga tccggcgggc
      361 cggcggagac tggatcaaac tgatgcacga cgacgactgg ttccgggatg cgcaggcctt
      421 gcaggtcttt gcagacggag tcaaaaagca tccggacggc aatttctttt tttcgggtta
      481 ccggaacgtc tttgagggaa aaaacaaaac ccaagagatg agcctcagcc gcccatggga
      541 aagattgctg aattccaatc ccgaaaattt actttcgaaa aatgtgatcg gaccgcccag
      601 cgtggtattg cataagaaaa ttccggaact cttgtatgac cggaacctga agtggctggt
      661 tgatattgat ttttatatcc ggttcatccg gtcccatccg atgccggtat tcctcccgga
      721 acagctgata gaggtcggga taagtgaaga tcaggtcacc caaagctgtt tccggaatcc
      781 cctggtcgag atacccgaac atctatacct tctgaacaaa atgggtaccc gcgctcttaa
      841 aaatatcata ctgtatgatg cgtactggcg gttcatccgg aacctgaatc tccggtcgac
      901 cgcggagatc cggaatgcgg gatatgctga acccgttccc cggatcattc ggtccatgat
      961 cggatttcag gccttcttcc ccgccgccgt tttaaaaacc ggaattattt ccaaaggtct
     1021 tatggccctg cacttcattt ttcaatatcc caaattagcc cgtggttaat ctctccatca
     1081 tcatcattaa ttataaaagt cttcccctca tccggaattg tttgcattcc atctatgccg
     1141 ccaggccggt cctcgatttt gaaattatca tagtaaataa tgaccctttg caagcagagg
     1201 atccgcaaat cacgcaaacc tatccggagc ttcaatggat cagcatggga tacaattccg
     1261 gttttgcaag agctaataac gaggggataa aacgggccgc cggagaggtc gttctcctgc
     1321 taaaccccga taccctcatt ccggaaacgg ccctggatag atgttaccgc aacctggttc
     1381 aatcggaata tattgcctgc ggagttcaat tgctgaatga agattccaca ccgcagatat
     1441 ccggaaacta ttttgtgaag ggaggcctga attatcttct tccgttgcct tatgccggca
     1501 aattcattaa atggctggct ggcctttttt cactaaaagc acctcatgtt gccctggctt
     1561 cttccgtcac ggaggtggac tggatcaatg gcgcgttcct catggttaaa aaagagggga
     1621 ttcaaaaagc gggaatgatg gatgaggatt tttttcttta tgcggaagag accgaatggt
     1681 gtgcgcggct gaaaaaggtg ggtagattat gcctcttcgg atccattacc gtttttcatt
     1741 tgcagggggt ttccgccaat gaagcttttt cttccagcgg caaagggtat tataatttgt
     1801 tcgaccggaa aggcctgcag atcatgcttt ccaacctcgt caggattaga aagcaattcg
     1861 gaaaattctg gttcttatgc gtactggcca tctacaccct ggattggccg gtttttctga
     1921 tcgggatcct gctttccttt gcttaccctg aaccggatag aacctattcc tttcatcaat
     1981 tccgggggtt tacgaaaaac ctgatctttc tctggtcaaa gacgccggta atccttttaa
     2041 acaggcctta tttttataaa gtgctttgaa gacccgattt ttttatttct ttcattttcc
     2101 gtcaatcggt tactttagct gaagaaacgg gatggattgg cctaatgaac aagcattgaa
     2161 aatgcaattc aaatatggaa cagaccagct gaccttatta aaagccattc agcttgcgga
     2221 agggagaata aaaggcattt taccggatga aacccgggag aatgtccgca agagccagtt
     2281 gcaggtcgct cagatcgttt ccagaaataa aaccgtgtat ggcatcaata ccggattcgg
     2341 tgcgctggcc aataccaaaa tttccgaaga tgacacccgg gtccttcagc aaaaaattct
     2401 tcagagccac agcgtaggcg ttggaaagcc catcgatccc ttcctggtca ggctgatgat
     2461 gatcacaaaa gttaatgccc tttgcaaagg attt
//
LOCUS       DASYCU010000149         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1776303, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000149 DASYCU010000000
VERSION     DASYCU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776303"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..596
                     /locus_tag="VGM24_02975"
     CDS             36..596
                     /locus_tag="VGM24_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY4154353.1"
                     /translation="MQYGIALYEDLRAYRSLYDLLSGSLHRFSYAFVKSNEAAEEIVS
                     DVFVKLWQIRDRLIEIENLKEYLYIITKNFSLNYIAKNYKHPVITLDSIDLETLIQIQ
                     SPEELCISADLVSKIRQVIQGLSPRCRMIFQLVKEENLKYREVASILNISVFTVRNQV
                     AIAVRKIGEALPDYMRSVAVHSPKAR"
     gene            672..1808
                     /locus_tag="VGM24_02980"
     CDS             672..1808
                     /locus_tag="VGM24_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY4154354.1"
                     /translation="MQEERFWLLISLRLTGEASPEERDELNGMLESRPGMGLKLDILE
                     HLWREKQGDPLANREDAFNKHLQRLSNHLSAPILKYDTEEPPVNRKAGAKRPARKYLW
                     WISGSAAAAALITSLLFFYPGERMKKAEPVAAQNTVSTKPGSKSKIQLPDGTQVWLNA
                     DSKITYDENFHGPSREVQLTGEAYFDVVKDKAHPFIIHTHSIDLKVLGTAFNVRSYAD
                     EKNTETSLIRGSVEITLRDNPDKKIILKPNEKLMVQNDDFSVLSGKAKEEKAGSGKGN
                     PEKAEEALMVLSKVHFEKKDSLPIETLWVKNKLAFDSESLENVAMKIERWYDVRIVIN
                     DERLKSKEYHAVFDDESLQQVMEALSSTGNFKYTINKKEVIITP"
     gene            1976..>2636
                     /locus_tag="VGM24_02985"
     CDS             1976..>2636
                     /locus_tag="VGM24_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4154355.1"
                     /translation="MKKNGCGVYNDTTHKIIKYLLMTKLAILFIFAISAQSFAHGYSQ
                     GISLKLDRVQLKKVFRVIEEQGTFRFVYKDEILPKNRRVSIQVHDASLEEVMGKLLEN
                     TSLSYKILNSNLIVITDGQAAGVAYESFAVNVKGIVTNSKGEPIGGVSVQEKETQNGT
                     ITKDDGSFSLDVVSNNSVLVFSHVGFRQLEVAVNNRHQLNISLEETGSNLNDVIVIGY
                     GE"
BASE COUNT          784 a          620 c          663 g          569 t
ORIGIN      
        1 gctagccatt gaaccaaact gccgacatac gggaaatgca atacgggatt gcgctttatg
       61 aggatctgag ggcataccgg tcgctttacg acctgctgtc gggcagccta caccggtttt
      121 cttacgcctt tgtaaaatcc aatgaggcgg cggaggagat cgtttcggac gtttttgtaa
      181 aactctggca gatcagggac cggctgatcg aaattgaaaa tctaaaggag tacctctaca
      241 ttatcaccaa gaatttttcc ctcaactata ttgcaaagaa ttataaacac cccgtcatca
      301 ccctggattc catcgatctg gagaccctga tccagatcca aagtcccgaa gaactttgca
      361 tttcagcaga cctggtcagc aaaataagac aggtcatcca ggggctctcg cctcgctgcc
      421 ggatgatttt ccagttggtg aaagaagaga acctgaaata ccgggaagtg gcctccatcc
      481 tgaacatctc cgtttttacc gtacgcaacc aggtggccat cgccgtccgg aagatcggcg
      541 aggccctgcc ggactacatg cggtctgtgg cggtccattc cccaaaagcc cgctaggctt
      601 atttctattt tttttgacct gccttggtac atcgccgcgc gaatcgctgt cttcattgta
      661 tagctattct tatgcaggaa gaaagattct ggttattgat cagcctccgg ctcaccgggg
      721 aagcatcccc cgaagaaagg gatgaactga atggcatgct ggaatcccgg cccggcatgg
      781 gtttaaaatt ggacatcctt gagcatttat ggagagaaaa acagggggat cccttggcaa
      841 accgggagga tgccttcaac aaacacctgc aaaggctgag caaccatcta tcggcgccga
      901 tattgaaata cgatacggaa gaaccgcctg tcaaccggaa agcgggcgct aagcggccgg
      961 cccgtaaata tctctggtgg atatccggca gcgccgccgc agctgccctg atcacttccc
     1021 tcctgttctt ttatccaggg gaaaggatga aaaaagcgga accggtcgcg gcgcagaata
     1081 cggtaagcac caaacccggc tccaagtcca aaattcagct gccggacggc acccaggtct
     1141 ggctgaatgc cgacagcaag atcacctatg acgaaaattt ccatggcccc tcccgggaag
     1201 tgcaattaac cggggaagcc tacttcgatg tggtcaaaga caaggcccat ccctttatta
     1261 ttcataccca ttccatcgat ttaaaagtat tgggcacggc cttcaatgtc cggtcctacg
     1321 cggatgaaaa aaatacggaa acctccctga tccgcggctc cgtcgagata acgctgcggg
     1381 acaaccctga taaaaagatc atcctgaaac cgaatgaaaa actaatggtt cagaacgatg
     1441 atttttcggt tttgtccgga aaggcaaagg aagaaaaggc cggatccgga aaagggaacc
     1501 cggaaaaagc agaagaagcc ctgatggtcc tgagcaaggt gcattttgag aagaaagaca
     1561 gtctcccgat agaaaccctt tgggtgaaaa ataaactcgc gtttgacagt gaaagcctgg
     1621 aaaacgtggc catgaaaatc gaacgctggt atgatgtacg gatcgtgatc aacgatgaaa
     1681 ggctgaagag caaggaatac catgcagtat tcgacgacga aagcctgcag caggtaatgg
     1741 aagcattgag cagcaccggc aattttaaat atacgatcaa taaaaaggaa gtgatcataa
     1801 cgccctgacc gggccctggc gaaaaaattt aataaacaac gaaacagacc gcttatgtag
     1861 gaaaggaaac gcaaaggata aaacaaaacg gagaatgctt gcaacattcc ccgtcaattg
     1921 ttaaaacaga aaatgctcat ggctgagcca ttccatcatt aatcaaaacc aagttatgaa
     1981 aaaaaacgga tgtggcgtat ataacgatac gacgcataaa atcatcaaat acctgctgat
     2041 gacgaaactt gccattttat tcatttttgc catttcagcc cagtcttttg cccatggata
     2101 cagccagggg atcagcctga agctggaccg ggtgcaactg aaaaaagtgt tccgggtgat
     2161 tgaggaacag ggcactttcc ggtttgttta taaggatgag atcctgccga agaacaggcg
     2221 ggtcagtatc caggtgcatg atgccagcct ggaagaagtg atggggaaat tattggaaaa
     2281 tacctcgctg tcctataaga tattaaacag caacctgatc gtaatcaccg acgggcaggc
     2341 ggccggagtc gcatatgaat cctttgccgt aaacgttaaa ggcattgtaa ccaattcgaa
     2401 aggagaaccg attggcggag tgagcgtgca ggaaaaggaa acgcaaaacg gcacgataac
     2461 aaaggatgat ggaagttttt ccctggacgt ggtgagcaac aattccgttt tggttttcag
     2521 tcatgtcgga ttccgccagc ttgaagtggc ggtaaataac cggcaccagc tgaatatatc
     2581 gctggaagaa accggatcga acctgaacga tgtgattgtg atcggctatg gggaaa
//
LOCUS       DASYCU010000150         4286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2354381, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000150 DASYCU010000000
VERSION     DASYCU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354381"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VGM24_02990"
     CDS             <1..746
                     /locus_tag="VGM24_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEY4154356.1"
                     /translation="EINLEYADQEAALRNLITNFPTSGEDFFKGDRNVLKTFRLDNGK
                     VVNVKSFRRPNIVNRIVYKYFRQPKASRSFHHSRKLLERGILVPKPVAFFENFDSYSI
                     ADSYFISEQIYPDVHFRKLLLNPEDPRYEEVVRHFAGLAHYMHENGIEFKDFSPGNVL
                     IMERGGRYDFYLVDLNRMDFHEKMSLEKRLKNFERLPPDERLMRIISDEYARIAGKPF
                     ELIYKGIAGFVRSFRKKFELRRKIKFWKR"
     gene            743..1810
                     /locus_tag="VGM24_02995"
     CDS             743..1810
                     /locus_tag="VGM24_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY4154357.1"
                     /translation="MRAKVRINNARRQVMDRLTRGIGADTVQQPTRLDGETIRRILVC
                     RPNHRLGNMLMITPLIQDLAHYFPEATVDLFVQGGAAVPIFRNYPQVSRIVQLPARPF
                     SHLLDYAGGWASMYFRRKYDLVINAYGGSSSGRLSTRFARARHRVFIEDRKTAGLAPG
                     EDDRHMAKYPAEQFRLQVAKAGLPPPASPVPHMDLLLDELELAEGRAALRSITGNDRP
                     SICLFTNATGDKLYPASWWDEFHSGLRAANPGLNIVEVLPLGAPSRLGGEIPRFFDRD
                     IRKVAAFFRNCALVVAGDSGIMHLASAALAPVLGLFKVTNTRVYEPYNPGSRAIDTRS
                     HPVSGIVDVANSMLRPVQTAV"
     gene            1867..4068
                     /locus_tag="VGM24_03000"
     CDS             1867..4068
                     /locus_tag="VGM24_03000"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEY4154358.1"
                     /translation="MKFSNFIHLCIGILFFVPGASAQTNRNASLPSSPQAKKKTPLDY
                     VNPNIGGIGRLLVATDPAVQLPQGSIRLCSNPWPEIYDRYLADKVFSFSLRDVVRYGT
                     KTIPSWIMASTGNIEVTPEKMASHYDHDFEKVTPYYSSLLLEDYDIQVEYTTTGQVAY
                     YKFSFPQSSASHILLGSNAKFSVVNESTVEGEETAGRRRFYFYAKFSKPFKSFGSWKG
                     GEVFPQSNTQSGDSVGVYANYATSKGEQVEVKIGVSNESIEQAQQNLEKEIPAWNFEA
                     VKNKAKDQWTQSLALFKVEGGTDDQLTKFYTGIYFSEGGERLEDRMNLRDKELLEQAR
                     KYAALSKDQLYPPVSSPGGMFQHGNIVFITEIYLRGIRDFDVQKAYENMKTEFMETTK
                     LPWRKGPATELDKFYIQNGFFPALPAGTKEWVPEVHDFEKRQCVTVTLQAAYESWCLA
                     QLAKALGKTDDYNFFIKHAHDYQNLFNTQTGFMSPKTADGNWVVPFNPTLSGGQGGRE
                     YFAEMNSWIYTWYVPHDIQGLMNRMGGREKFNNKLDSLYTVQYQTSKYRFLDQFPDMT
                     GLIGNYAQGNEFSRLIPYLYNYSGAPWKTQKRVRDIMSIWYGTGPTGICGDEDTGLMS
                     DWYVCSAMGIYSDPMIPAYPVWLIGSPVFRKSTITLGNNKTFVIEAKNVSAQNKYVQS
                     ATLNGEPLNKPWFDHSTLLKGGTLVLNMGPRPNKEWGSSPEAAPPSMSGAE"
     gene            complement(4093..>4286)
                     /locus_tag="VGM24_03005"
     CDS             complement(4093..>4286)
                     /locus_tag="VGM24_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154359.1"
                     /translation="LRLAKAFGTTAELWLNMQRNFDLWNASRKIKLSQVKVLRRAKTD
                     TTDRSVLKGKTLRPSAAHA"
BASE COUNT         1096 a         1117 c         1116 g          957 t
ORIGIN      
        1 gcgaaataaa cctggaatac gcggatcagg aagcggcgct ccggaatttg atcacaaatt
       61 tccccacttc gggggaagat ttcttcaaag gagaccggaa cgtgctcaag actttccgcc
      121 tggacaacgg caaggtggtc aacgtgaaat cctttcgcag gcccaatatc gtgaaccgga
      181 tcgtgtacaa atatttccgc cagccgaagg cgagccgttc ctttcaccat tcccgcaaac
      241 tgctggaacg cgggatcctg gtacccaaac ccgtagcttt tttcgagaat ttcgattcct
      301 actccatcgc cgacagctat ttcatcagcg aacagatcta cccggatgtc catttccgga
      361 aattgctgct caatcccgag gacccgcggt acgaggaagt cgttcgtcat ttcgcgggac
      421 tggcgcacta catgcacgag aacggcatcg agttcaagga tttttcgccc ggtaacgtgc
      481 tgatcatgga aaggggcggc cgctacgatt tctatctcgt cgacctgaac cgcatggatt
      541 ttcacgaaaa gatgagcctg gaaaaaagat tgaagaattt tgaaaggctg ccgcccgatg
      601 aaagactgat gcggatcatc agcgatgaat atgcgcgcat cgccggcaaa cctttcgagc
      661 tgatctataa aggcatcgcc ggtttcgtcc gcagtttccg caagaagttc gaacttcggc
      721 ggaaaatcaa attctggaaa cgatgagggc gaaagtacgg atcaacaatg ccaggcgaca
      781 ggtcatggac cggctcacgc ggggcatcgg cgcagacacg gtgcagcaac caacccgtct
      841 cgatggcgaa accatccggc ggatcctggt gtgccggccc aaccaccggc tcggaaacat
      901 gctgatgatc acccccctta tccaggacct cgcgcattat tttcctgaag ccaccgtgga
      961 tctgttcgta cagggagggg cggccgtacc gatcttccgg aactatcccc aggtatcgcg
     1021 gatcgttcag ttgccggcac ggcccttcag tcacctcctg gattatgcgg ggggctgggc
     1081 ttctatgtat ttccgccgta aatacgattt ggtcatcaac gcctacggcg gatcttcttc
     1141 gggcaggctc tctacgcgat tcgcgcgggc ccgccaccgg gtgttcatcg aagaccggaa
     1201 aaccgccggc ctggctccgg gggaggacga ccggcacatg gcgaaatacc cggccgagca
     1261 attccggctt caagtggcaa aagccggcct cccgccgccc gccagtccgg tgccgcacat
     1321 ggatctgttg ctagatgaac tggagctggc cgaaggacga gctgccctcc ggtcgatcac
     1381 cggaaacgac cgtccgtcga tctgcctgtt cactaatgcc accggcgaca agctgtatcc
     1441 ggcttcctgg tgggacgaat tccactccgg tctgcgcgct gcaaaccccg ggttaaatat
     1501 cgtggaagtc ctgcccctgg gtgccccgtc ccggcttggc ggggagatac cccgcttttt
     1561 cgaccgcgat atccgcaagg tggcggcctt cttccggaat tgcgccctcg tcgttgccgg
     1621 cgatagcggc atcatgcacc tggccagtgc ggcgctcgct cccgtcctgg ggctattcaa
     1681 ggtgacgaac acgcgggtgt acgaaccgta caatccggga agccgcgcaa tagatacccg
     1741 ttcacacccg gtgtccggaa tcgtagacgt ggcgaactcc atgctgcggc cggtgcagac
     1801 ggctgtctaa ccagaaaggc tgatccggtg ggaatatcga aaaaaaaatt ttcctaattt
     1861 gtccaaatga aattttcaaa ttttattcat ttatgtattg gaattctatt ttttgttccg
     1921 ggcgcatccg cccaaacgaa ccggaatgct tcactccctt cctccccaca ggcgaaaaag
     1981 aaaacgccgc tcgattatgt gaaccccaat atcggaggca tcggccgcct gctggtagcg
     2041 actgacccgg cagttcagtt gccgcaggga tctatccggt tatgttcgaa cccctggcct
     2101 gaaatttatg accggtacct ggcagataag gtattcagtt tttccctccg ggatgtggtt
     2161 aggtatggta caaaaacaat accgtcctgg atcatggcca gcaccgggaa tattgaagtg
     2221 actccggaaa aaatggcatc gcactatgac cacgattttg aaaaagtaac gccttactat
     2281 tcctctctct tgctggagga ttatgatata caggttgaat ataccacgac cggtcaggtg
     2341 gcctattata agttcagctt tccgcagagt tccgcttccc acattctgct ggggagcaat
     2401 gcaaaattca gcgtcgtgaa tgaaagcacg gtggaaggag aagaaacggc cggaagacgc
     2461 cgtttttatt tctacgcaaa attcagcaag ccctttaaat catttggctc ctggaagggc
     2521 ggtgaagtct ttccccaatc caacacgcaa agcggcgaca gcgtgggcgt ttacgcaaac
     2581 tatgccacat caaaaggaga acaggtagaa gtaaaaatcg gggtttccaa tgaaagtatt
     2641 gaacaggcgc aacaaaacct ggaaaaggaa atcccggcct ggaactttga agccgtaaaa
     2701 aacaaggcaa aagaccaatg gacgcaatcc ctggccctgt ttaaagtgga aggcggaacg
     2761 gatgatcagc ttacaaaatt ttacaccggt atttactttt cagaaggcgg tgaacggctg
     2821 gaggacagaa tgaacctccg ggataaagaa ttactcgagc aggcgcgaaa atatgccgcc
     2881 ttatcgaagg atcagctata cccgccagtc tcctccccag gtggcatgtt ccagcatggc
     2941 aatattgttt ttataacgga gatctacctg agaggcatcc gtgatttcga tgtgcaaaaa
     3001 gcctacgaaa atatgaagac ggagtttatg gagacgacca aattgccctg gcgtaaaggg
     3061 cctgccacgg aactcgataa attttatatt caaaatggat ttttcccggc attgccagcg
     3121 ggcacaaagg aatgggtgcc cgaagtgcat gattttgaaa aaagacaatg cgttaccgtt
     3181 accctgcagg ccgcctacga gagctggtgt cttgcgcagc tggcaaaggc cctgggcaaa
     3241 acggatgact ataatttttt tataaaacac gcgcatgatt atcaaaacct gtttaatacc
     3301 caaaccggtt ttatgtcgcc taaaactgcc gatggcaact gggtggttcc tttcaatccg
     3361 accttatccg gagggcaggg gggaagagaa tatttcgctg aaatgaacag ctggatatat
     3421 acctggtatg ttccccacga tatccaggga ctgatgaacc ggatgggcgg gcgggaaaaa
     3481 tttaataaca agctggattc tctttacacc gtgcaatatc aaacgtcgaa ataccggttt
     3541 ctggatcagt tcccggatat gacgggttta attggaaatt atgcgcaggg aaatgaattc
     3601 tcccggctga tcccctatct gtataattat tccggcgctc cctggaaaac acaaaagcgg
     3661 gtaagggata tcatgagtat ttggtatggc acaggtccga cgggaatatg cggagatgag
     3721 gataccgggt tgatgtctga ctggtacgtc tgcagcgcca tgggcattta cagtgacccg
     3781 atgatccccg cctatcccgt atggcttata ggaagcccgg tatttagaaa atctaccatt
     3841 accctgggca acaacaaaac gtttgtgatc gaagcaaaaa atgtatcggc acaaaacaaa
     3901 tacgttcaat ccgctacttt gaacggcgag ccgctgaata aaccctggtt cgaccattca
     3961 acacttctta aaggcggaac gctggtgctg aatatgggac cccggccgaa taaggaatgg
     4021 ggaagctctc cggaggcggc gcctccatca atgtcggggg cagaatgagc tgactatttc
     4081 gctcgtaggt cgctaggcgt gagccgcaga aggtcgcagc gtttttcctt tcaaaaccga
     4141 cctatcagta gtatcagttt ttgcgcgacg caaaactttc acctgcgaaa gctttatttt
     4201 ccggctcgca ttccacagat caaaattgcg ctgcatattc agccacagtt ccgctgtagt
     4261 accaaaagcc tttgccaaac gtaagg
//
LOCUS       DASYCU010000151         5420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2936308, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000151 DASYCU010000000
VERSION     DASYCU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2936308"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VGM24_03010"
     CDS             <1..500
                     /locus_tag="VGM24_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY4154360.1"
                     /translation="FVDGPEVDSIFYETPYFLEPQKSGEKAYVLLREALKKSGKVGVG
                     NYVMRNRESLCILKPQDNVIILNKIRFAQEIRNVNEINVPGKTSIKAGEMQMAQALID
                     QLSGKFDITKYKDSYSDELLKLIHAKAKGKKTEAPKLRVVHSRSKDLMSQLKASLTVK
                     KKKAS"
     gene            complement(503..>5420)
                     /locus_tag="VGM24_03015"
     CDS             complement(503..>5420)
                     /locus_tag="VGM24_03015"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY4154361.1"
                     /translation="GLEVSIFPVIHALTEEELPDEEKKWLEGLRKLVLETGRRAKERL
                     LTIQALMKSCEEFADLKFDFLYDRSQRLLSIGYNVTDHRRDNSFYDLLASESRFTIFV
                     GIAQGKLIQDTWFALGRQLTNPGTSPVLVSWSGSMFEYLMPLLVMPTFENTLLDQTYK
                     TTVQKQIEYGKKRDVPWGISESGYNMVDASLNYQYRAFGVPGLGLKRGLGEDLVIAPY
                     ATVMSLMVAPEESAENLLDMDAEGFSGRYGMFEAVDYTASRLTRGQSYAIVRSFMAHH
                     QGMSFLSIAYLMLDKPMQKRFEAEAQFRAVMLLLQERIPHATEFYSPSVHVSDISTTS
                     QDIQMRIIKSPNTPVPEVQLLSNGRFHSMICNSGSGYSRWKDIAVTRWREDISCDHYG
                     TYCFIRDLDNETVWSAAYQPSLNMAENYEVVFSQGRAEFRRQEQNLETHTEIVVSSED
                     DVEMRRIRINNRSRKKRYLEVTSYAEVVLAPPAADAAHPAFSNLFVQTEILPQRDAIL
                     CTRRPRSAGDSQPWMFHLMKVHNAEIKDISYETDRSVFTGRTRSIHEPDALKKTQRLT
                     GTQGAVLDPIVSIRYRFVIEPHDTVTINMVLGMAESRETATGLIEKYQDRPLTDRAFE
                     LAWTHSQVVLRQIDATEADAQLYARLVGSVIFPNASLRADPSVIIRNRRGQSGLWSYS
                     ISGDLPVVLLQIQDSANIELARQLVQAHAYWRLKGLLVDLVIWNEDYGGYRQALQNEL
                     ISLISPGIISDVRDKPGGIFIRSGEQVSQEDRILFQSVARIIISDTLGTLEEQISRRT
                     KVRPVIPYFTPTKFYASLDSALETPKDLTFFNGTGGFSKDGKEYVIITGEQNVTPAPW
                     CNVIANPVFGTVVSESGQAYSWMANAHEYRLTPWNNDAVSDFNGEAFYIRDEESGKFW
                     SPAPLPHPGKSHYVSRHGFGYSEFHFAEDGIYSEMTVFVDTEDPVKFIAFKIKNNSAR
                     VRKLSLTGYVEWVLGDLRTKTQMHISTEVNTETGILLAGNAYNAEFGNYVSFFDVDDP
                     GRTFTCDRIEFLGRNGTYKNPEALNKAKLSGRAGAGMDACAAIQSVLDLADGEERELV
                     FRLGSGPDTYSALKTAKKFRGSLTAQNTLEKVKAFWQKTLGVVQVNSPDPTINYLCNG
                     WLNYQTLASRIWGRSGFYQSGGAFGFRDQLQDVLSLLHNRPDLVRQQIILSSSRQFKE
                     GDVQHWWHPPMGRGVRTACSDDYLWLPYVTSRYISHTGDESILNEQTRFIEGRLLNPG
                     EESYYELPAVSEQSASLYEHCVRSIKHGLRFGEHGLPLMGSGDWNDGMDKVGDQGKGE
                     SVWLGFFLYDVLVRFEEIARLKRDTDVGDLCKKEARLLSKNIEAGAWDGDWYRRAYFD
                     NGTPLGSAENDECKIDSIPQSWSVISGGGDPERSRIAMDSAYSKLVKKDTEIIQLFDP
                     PFNHSELDPGYIKGYLPGVRENGGQYTHAAIWLIMAAAAQRDKERTYELIRLINPLNH
                     GINTDEIKKYKIEPYVIAADVYAVENHLGRGGWTWYTGSAGWMYQLLLESFFGLKRNG
                     NKLSFEPCLPDHWPQFTVSYQYGESVYQLDFEQQETWNKTTILLDDAMQEGNAVFLVN
                     DRETHHVRIRLPLAIGKTLKQVPEAVSV"
BASE COUNT         1255 a         1485 c         1353 g         1327 t
ORIGIN      
        1 aatttgtgga cggaccggaa gtggattcga ttttttatga aacgccctat ttcctggaac
       61 ctcagaagtc gggcgaaaaa gcctatgtcc tgctcagaga agcgttaaag aaaagcggaa
      121 aagtgggtgt ggggaattat gtgatgcgga acagggagtc gctttgtatt ttaaaaccgc
      181 aggacaatgt gatcatcctg aataagatcc gctttgccca ggagatccgc aatgttaatg
      241 agatcaacgt gccgggcaaa acaagtatca aagcgggtga aatgcaaatg gcccaggcgc
      301 tgatcgacca gctcagcgga aaatttgata tcactaaata taaagacagc tattcggatg
      361 aactgctgaa gcttattcat gccaaggcga agggcaaaaa gaccgaagcg cccaaactgc
      421 gcgtggttca cagcagatcc aaagacctga tgtcccagct gaaagccagc ctgaccgtga
      481 aaaagaaaaa agcatcctga ccttaaacgg aaaccgcttc cggcacctgt ttcagcgttt
      541 tcccgatggc caggggtaac ctgatccgta cgtggtgcgt ttcccggtcg ttcaccagga
      601 ataccgcatt gccttcctgc atcgcatcat ccagcaatat ggtcgtttta ttccaggttt
      661 cctgctgttc aaaatccaat tgataaaccg attccccata ctggtaactg acggtgaact
      721 gcggccagtg atccggcagg cagggctcga aagaaagttt gttcccgttt cgcttcagtc
      781 cgaaaaatga ttccagcaac aactggtaca tccacccggc agaaccggta taccaggtcc
      841 atcctcccct tcctaggtgg ttttccacgg catacacatc tgcggcaatt acatagggct
      901 ctattttata ctttttaatt tcgtctgtgt ttatcccatg gttcagcgga ttgatcagcc
      961 ggatcagttc atacgtccgt tccttatcgc gctgggcggc cgctgccatg atgagccata
     1021 tagcggcgtg cgtgtattgc cctccgttct cccgcactcc cggaaggtat ccctttatgt
     1081 atcccggatc cagttcggaa tgattgaagg gcgggtcaaa taactggatg atttcagtat
     1141 ctttttttac cagttttgaa taggcggaat ccattgcaat ccggcttctt tccggatcgc
     1201 cgcctcccga aatgacggac cagctttgcg gaatggaatc aatcttacat tcgtcgtttt
     1261 cggcggatcc cagcggcgtt ccattgtcaa aataagcccg gcggtaccag tccccgtccc
     1321 aggctccggc ctctatattc ttgctgagta agcgggcttc cttcttgcag agatctccga
     1381 catcagtatc tcttttcagc ctggcgattt cttcaaagcg taccagtacg tcatacagga
     1441 aaaagcccag ccatacactc tccccttttc cctggtctcc cactttatcc attccatcgt
     1501 tccaatcgcc ggatcccatc agcggcagtc cgtgttctcc aaaccttaat ccgtgtttta
     1561 tggagcggac gcagtgttca tacaggctgg cggattgttc cgatacggca ggcaattcat
     1621 aataggattc ttcccccggg ttcaatagcc tgccttctat aaaccgggtc tgttcattca
     1681 aaatggactc atcgccggta tgggaaatat atcttgatgt tacatagggc aaccacagat
     1741 agtcatccga acaggctgtc cggactccgc gtcccatggg tggatgccac cagtgctgca
     1801 catctccttc tttaaactgc cgcgacgaac tgagtatgat ttgctgacgc acaaggtccg
     1861 ggcggttatg cagcagggac agaacgtcct gcaactgatc gcgaaagccg aaagcgccgc
     1921 cggactggta aaatccgctt cttccccaga tgcgggatgc gagtgtctgg taattcagcc
     1981 agccattgca taaatagttg atggtcggat cgggcgaatt cacctgcacg acgcccagcg
     2041 tcttttgcca gaaagccttc actttttcca gcgtattctg tgccgtgagc gaaccgcgga
     2101 atttttttgc ggtcttcagt gcggaatagg tatccggccc tgaacccagc ctgaatacaa
     2161 gctccctttc ttctccgtct gccaggtcca gcacggactg aatagctgcg caggcatcca
     2221 tacccgcgcc cgccctgccg gatagtttgg ctttgttcaa cgcttccgga tttttatagg
     2281 tgccatttct tcccaggaat tcaatccggt cgcaggtgaa ggtccttccc ggatcatcca
     2341 catcgaaaaa gctgacgtaa tttccaaatt ccgcgttgta tgcattcccc gccagcaaaa
     2401 taccggtctc ggtattcact tccgtgctga tatgcatctg cgttttggtc ctcaggtcac
     2461 ccagcaccca ttccacatac ccggtaagcg ataattttct gacccgggcg gaattgttct
     2521 tgatcttgaa tgcgataaat ttcaccggat cttccgtgtc tacaaatacg gtcatctccg
     2581 aataaatccc gtcttcggcg aaatggaatt cactgtatcc gaacccatgg cggctgacat
     2641 aatgtgattt tccgggatga ggcaaaggag cgggtgacca gaatttcccg ctttcttcat
     2701 cccggatata aaatgcttcc ccgttaaaat cgcttacggc atcattattc catggggtca
     2761 accggtattc atgcgcattg gccatccagg aataagcctg tccgctttcg gaaaccaccg
     2821 taccaaatac cgggttggct attacgttgc accagggagc gggcgtcacg ttctgctcgc
     2881 cggtaatgat cacatattct tttccgtctt tggaaaaacc gccggtgccg ttgaagaacg
     2941 tcagatcctt cggcgtttcc agtgcggaat ccagcgatgc gtaaaatttt gtcggcgtga
     3001 aataagggat cacgggccgg accttggtac ggcggctgat ttgttcttcc agtgtcccca
     3061 gcgtatcgga aataatgatc ctcgccacgc tctgaaacag gatgcggtct tcctgggaaa
     3121 cctgctctcc cgaacggata aatattcctc ccggcttatc ccggacatca ctgatgattc
     3181 ccggggaaat aaggcttatg agctcattct gcaatgcctg gcgataccct ccgtaatctt
     3241 cattccagat caccaggtca accagcaatc ctttcaggcg ccagtaggca tgtgcctgca
     3301 ccagttgtct cgccagttca atattggctg aatcctggat ctgcagcagc accacgggca
     3361 ggtctccgga aatggaataa ctccaaagcc cggattgtcc tctccggttc cggataatga
     3421 ctgacggatc tgcccgcaaa gaggcgttgg gaaaaatgac agacccgacc agccgggcgt
     3481 ataactgggc atccgcctcg gtggcatcaa tctggcgaag caccacctgg ctgtgcgtcc
     3541 aggccagttc aaacgcccgg tccgtcaacg ggcggtcctg gtatttttct atcaggcctg
     3601 tggctgtttc gcggctttcc gccataccga gcaccatgtt gatggtaacc gtatcatgcg
     3661 gctcaatcac gaaccggtag cggatcgaaa cgatcggatc cagcaccgct ccctgggtac
     3721 cggtgagccg ttgcgttttt ttaagggcgt ccggttcatg aatgctgcgc gttcttccgg
     3781 taaaaacaga ccggtccgtt tcataggaaa tatctttgat ctcggcgttg tgcactttca
     3841 tcagatgaaa catccagggc tgactgtcgc cggcagaacg cggccttctc gtgcacagga
     3901 tggcatcccg ctgcggcagg atctccgtct gaacaaacag attgctgaac gccgggtgag
     3961 ccgcatccgc agccggcggg gccagcacca cttccgcata gctggtcact tcgaggtatc
     4021 tctttttcct cgagcggttg ttgatccgga tccgccgcat ctccacgtcg tcttcagaag
     4081 aaacaacgat ctcggtgtgc gtttccaggt tttgttcctg ccttctgaac tcggcccttc
     4141 cctgggaaaa gaccacttca tagttctccg ccatgttcag cgaaggctga taagccgcgg
     4201 accagaccgt ttcattgtcc aggtcgcgga taaaacaata ggtgccgtaa tgatcgcagc
     4261 tgatgtcctc acgccaacgg gtaaccgcaa tatccttcca gcggctgtac ccgctgccgg
     4321 aattacagat catggaatga aatcttccgt tcgacagcaa ctgaacttcc ggaacgggcg
     4381 tattcggcga tttgatgatc cgcatctgga tgtcctggga cgtggtgctg atatcggaaa
     4441 catgcacgga aggagaatag aattcagtgg catgcgggat cctttcctga agcagtaaca
     4501 tcacggcgcg gaattgcgct tcggcttcaa atcgtttctg catgggtttg tccagcatca
     4561 ggtaagcaat ggaaaggaag gacattccct ggtgatgcgc catgaaagac cgcacgatgg
     4621 cataggactg accccgggtg agccgggagg cggtataatc caccgcttcg aacattccat
     4681 atcttccgga aaatccttcg gcatccatat ccagcaggtt ttccgccgat tcttcggggg
     4741 ccaccatcag cgacatgacg gtggcatacg gggcaatgac gagatcctcg cccagcccgc
     4801 gcttgaggcc gagtcccgga acgccgaagg cgcggtactg gtaattgaga ctggcatcaa
     4861 ccatattgta ccccgattcc gaaatacccc agggaacatc ccgctttttt ccatattcga
     4921 tttgcttctg tacggtcgtt ttataggttt gatccagcag ggtattctcg aatgtcggca
     4981 tcaccagcaa aggcatcagg tattcaaaca tggatccgct ccaggaaaca agcaccggcg
     5041 aagtgcccgg gttggtaagc tggcgtccca gggcaaacca ggtatcctga atgagctttc
     5101 cctgagcgat gcccacgaaa atagtgaacc gggattctga agccagcaga tcataaaaac
     5161 tattgtcgcg gcggtggtcg gttacgttgt acccaatgga tagcaaacgc tgcgacctat
     5221 cataaaggaa atcaaatttc agatcggcaa attcctcgca ggacttcatc agggcctgga
     5281 tggtcagcag gcgttcctta gcccggcggc cggtttcgag caccagtttg cggagcccct
     5341 ccagccattt tttttcttca tccggaagct cttcttccgt taaggcgtga atgaccggga
     5401 aaatagaaac ttccagaccg
//
LOCUS       DASYCU010000152         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2936357, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000152 DASYCU010000000
VERSION     DASYCU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2936357"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGM24_03020"
     CDS             complement(<1..619)
                     /locus_tag="VGM24_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY4154362.1"
                     /translation="MRFLLLILSAGLAQFILAQDAPANGVHQHIHYSPLAFSNKFSLH
                     VPPVLRLRSGDTVSTETIDALGFDKQGVKRQKGGNPLTGPFYIENALPGDVLAIALIR
                     VSLNRSYAYTTEAFGSRSLPKTYLSRSKKAKLVRWKLDIRNVFASPDTNYEHLSRFKV
                     PLAPFLGCIGLAPTAKKEEILSFFSGPIGGNLDYSRVTQGATVYLP"
     gene            638..1531
                     /locus_tag="VGM24_03025"
     CDS             638..1531
                     /locus_tag="VGM24_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4154363.1"
                     /translation="MALFFNLFQKINMATSKKTVPEQKQSLPGSEQEMDPKPVSDDPE
                     YKPTGKLKDKVVLITGGESGIGKAVAILFAKEEAKLAIVYLKEDEDAADTRKQIEKYG
                     GRALFIRGDISDAGFCREQVLACIREYGGIDILVNNAAVQHPVKKLEDLKDEDLLHTF
                     QVNILSMFRITREALPYMKKGSCIINTTSVTAYHGSAGLLDYSSTKGAIVSFTRSLAL
                     NLAEREIRVNGVAPGPVWTPLIPASFEAGKTSRHGSDVPMKRPGQPVEIAPSYLFLAT
                     GASSYMSGQILHPNGGTIVNG"
     gene            1549..2046
                     /locus_tag="VGM24_03030"
     CDS             1549..2046
                     /locus_tag="VGM24_03030"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY4154364.1"
                     /translation="MDILIQVFGEDKNLTVLQMVFRGVVVFLIALVLIRISGRRSFGI
                     RTSLDNIISMLLGAVLSRAVVGASPFLPVLCSCLGIVLLHRAFGWMLIRSDRFARLVE
                     GSRIQLFKNGRFNEENMRKGLVCKEDIMQGVRKSALTENLDEIEAVFMERNGEITAIK
                     KQTGQ"
     gene            2047..>2723
                     /locus_tag="VGM24_03035"
     CDS             2047..>2723
                     /locus_tag="VGM24_03035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4154365.1"
                     /translation="MDLHSDSPYWLMKDGMLHTYPSLQANKKTDIVIMGAGITGAIIG
                     YHLNKAGLEVMLVDKRHAATGSTCASTALLQYEIDIPLIRLVEKAGEKDAVRSYRLCL
                     DALTSIKNISEHIGHKSLEAKPSLQYASFKKDAGPHYQEYLLRKKYNFPVDWLSEKEI
                     KRYYHLSAPGAILSAVAAQTDPYELADDLLQYLVKHGSEVYDATEIKKIVRQERKIEL
                     TTGEGYV"
BASE COUNT          707 a          675 c          734 g          607 t
ORIGIN      
        1 ccggcaggta aaccgtcgcc ccctgcgtta cccggctata atcgagattg cctccaatcg
       61 gtccggagaa aaagctcagg atctcctcct ttttggccgt gggagccagt ccgatacaac
      121 ccaaaaaagg agcgaggggc actttaaaac gggaaagatg ctcataattc gtatccggtg
      181 aagcgaacac gttccggata tccagtttcc agcgcaccag cttcgctttt tttgaccggc
      241 tgagatatgt tttgggcagg cttcttgaac caaaagcctc ggtggtgtag gcataggaac
      301 ggttcaggga aacgcggatc agggcaatgg ccagtacatc tcccggcagc gcattttcaa
      361 tataaaacgg tccggtcaac ggatttccgc ctttctgacg tttaacgccc tgcttgtcga
      421 atccgagagc atcaatggtt tccgtgctga cggtatctcc cgaccggagc ctgaggaccg
      481 gcggaacgtg gagcgaaaat ttattggaaa aggcaagggg agaataatgg atgtgctgat
      541 gaactccatt tgccggagca tcctgggcca gtatgaattg ggccaatccg gcagataaaa
      601 taagcagtag gaatcgcata cggccaaaag tacaacgatg gcactatttt ttaacctgtt
      661 tcagaaaatc aatatggcta catcgaagaa aacagtacct gaacaaaaac aatctttgcc
      721 cggctccgaa caggagatgg atcccaaacc cgtgtccgac gatccggaat acaagcctac
      781 cggaaagctg aaggacaagg tcgtcctgat aaccgggggc gaaagcggaa tcggaaaagc
      841 ggtggccatc cttttcgcca aggaagaagc caaactggcg atcgtttatc tcaaggagga
      901 cgaggacgct gcggataccc ggaaacagat tgaaaaatat ggcgggcggg ccctttttat
      961 ccggggcgat atttccgatg ccggtttttg ccgcgaacag gtgctggcct gcatccggga
     1021 atacggggga attgatatcc tggtgaataa tgccgccgtg cagcatcccg tgaagaaact
     1081 ggaagacttg aaggacgagg acctgctcca tactttccag gtgaatattc tttccatgtt
     1141 ccggatcacg cgggaggccc ttccctatat gaagaaagga agctgcatca tcaataccac
     1201 ttcggtcact gcttaccatg gaagcgcagg attactggat tattcctcta ccaagggagc
     1261 cattgtttcc ttcacgcgga gccttgcgct caacctggcc gagcgggaga tccgggtgaa
     1321 tggcgtcgcg ccgggtccgg tctggacgcc gctgatcccg gccagctttg aagcggggaa
     1381 aaccagcagg cacggatcgg atgttccgat gaaaaggccg ggccagccgg tggaaattgc
     1441 gccttcgtat ttattcctgg ctaccggggc ctcctcgtat atgagcgggc agattcttca
     1501 tcctaacggc ggaacgattg tcaacgggta atcttcaata aaaaatccat ggatatattg
     1561 atccaggtat tcggggaaga caagaacctt actgttttac aaatggtatt ccggggagtt
     1621 gtcgtgtttc tcattgccct ggtgcttatc cgtatttcag gcaggcgttc ctttggcatc
     1681 cgcacttcac tcgacaacat catttccatg ttgctcggag cggtccttag ccgggccgtc
     1741 gtcggcgctt ctccttttct tccggtactg tgttcctgcc tgggcatcgt tttgcttcac
     1801 cgtgcattcg gctggatgct gatcaggagt gaccgctttg cccgcctggt agagggttcc
     1861 cggatccagc tgtttaagaa cggccgcttc aatgaagaga atatgcgaaa gggactggtg
     1921 tgcaaagagg atatcatgca gggcgtgcgg aaatcggcac tgacggaaaa cctggacgaa
     1981 attgaagctg tttttatgga gcgtaacggg gagatcactg ccataaaaaa acaaaccgga
     2041 caatagatgg acctgcacag cgacagcccc tactggctaa tgaaggacgg catgctccat
     2101 acctatcctt ccctccaggc aaataaaaaa acggatattg tcatcatggg cgcggggata
     2161 accggcgcga tcatcgggta ccacctcaac aaagccggtt tggaagtgat gctggtggat
     2221 aaacgacatg cggcaaccgg cagcacctgc gccagcacgg cgctccttca atacgagatc
     2281 gatatccctc tgatccggct ggtggaaaag gccggagaaa aagatgcagt gcgaagctac
     2341 cggctatgcc tggacgcttt gacttcaata aaaaatattt cggagcatat aggtcataaa
     2401 tccctggaag caaaacccag tctccagtat gcatccttta agaaggacgc cgggccgcat
     2461 taccaggaat atcttttacg gaaaaaatat aattttccgg tggactggct ttcggaaaaa
     2521 gaaataaaac gttattatca tctgtccgcc ccgggagcca tcctttctgc ggttgccgca
     2581 cagaccgatc cttacgaact tgccgatgac ctgctccagt acctggtcaa acacggatcg
     2641 gaagtatatg atgcaacgga aataaagaag atcgtgcggc aggaaagaaa aatcgagctt
     2701 actaccggtg agggatatgt tat
//
LOCUS       DASYCU010000153         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_909123, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000153 DASYCU010000000
VERSION     DASYCU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909123"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VGM24_03040"
     CDS             <1..768
                     /locus_tag="VGM24_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4154366.1"
                     /translation="TEKGGTIGYYLSRKNIAVVDAEFPEQSKHLIDALQKDPGKPFEI
                     LINTHHHGDHTSGNISFRGIVKHVAAHSNSLINQRRVAEEQKKEDQQLYPDLVYGDAW
                     EYKKLKEIIKTYYFGPAHTNGDSIIHFPHANIAHMGDLVFNRRWAFIDRTAGASVKNW
                     IVVLDKAQQLFDKDTLFIYGHAFDPDKVTGYKEDLKAMQDYLSRLLDFVDKQIKAGKT
                     REEILKAKAIPGVTEWQGDGIERGLEAAYDEQIQGII"
     gene            complement(761..1255)
                     /locus_tag="VGM24_03045"
     CDS             complement(761..1255)
                     /locus_tag="VGM24_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY4154367.1"
                     /translation="MQNPLTITLQNWQKVSGDHQKKYKDFLAKANKNKVLKRLPDLHE
                     RAFRQIDCLQCARCCKRYSPRFKTPDIKRISRRLQMKEGDFIEKYLQLDEEGDYVLIS
                     TPCAFLGNDNRCSIYEDRPSDCRRFPYTDEDVILKRPKLTQMNSSFCPIVVSVLEGLL
                     DEPK"
     gene            1282..2211
                     /locus_tag="VGM24_03050"
     CDS             1282..2211
                     /locus_tag="VGM24_03050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY4154368.1"
                     /translation="MTGSLREQFLILISTPLYIFIIGLELLLSHFQKRRLYSWKDTAS
                     NVYLMLMNAIIDLSFRAIYVAILVQLFDHKLISWKMGISYWVMLLLAEDFLYYWLHRF
                     DHEVRLFWAVHVTHHSSEYMNFTVGFRSSVFQPLYRFIYFIPLAWLGFKPLDIVFIYS
                     ATQIWGIFVHTDLIRKMGWLEYFLVTPSHHRVHHASNPKYLDKNMGMFLIVWDQLFGT
                     FQPELEDQEYQEKRYGLTKPLDRSSPGNLVFHEWRAIRKDLARTDISWKDKWYYVFGP
                     PGWSHDGSRQTSRQLLEKEELKVPEPGEAFQTR"
     gene            2268..>4363
                     /gene="gcvP"
                     /locus_tag="VGM24_03055"
     CDS             2268..>4363
                     /gene="gcvP"
                     /locus_tag="VGM24_03055"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY4154369.1"
                     /translation="MNLFEKQVNEFARRHIGSNETETRQMLAQIGVESLDALINKTIP
                     QTIRHPLPLQVTPAVSEYEYLKLLREIASENKLFKNYIGQGYYDTITPSVILRNVFEN
                     PGWYTQYTPYQAEISQGRLESLLNFQTIVSDLTGLPIANASLLDEGTAAAEGMIMFFN
                     QKNKDHQRVTVPRFFVDQAIFIQTRDVLATRAEALGIELVTGNFEQVKIDASFFGAIL
                     QYPDSNGNISDHRAFIRKVHDAGAFVVMATDLLALTLLTPPGELGADVAVGSAQRLGV
                     PVGFGGPHAAFFAAAEQFKRAIPGRIIGVSVDAQGNRALRMALQTREQHIKREKATSN
                     ICTAQALLANMAAMYAVYHGPAGLKEIATRICVLAQGLSEGLARLGFENGHNHFFDTV
                     QVRSDNTEQLREIAESRQINFFYGESGKVTISLNESSTQADVLDILSVFAQSRDIDEA
                     ATIFQYDGHLESIPATLNRSSAFLTHPVFNAHHSETEMMRYLKSLENRDLSLNSSMIP
                     LGSCTMKLNAASELIPVSWPEFSRIHPFAPPDQWKGYQRIISELEEWLSIITGFAATS
                     LQPNSGAQGEYAGLLAIRSYHAAHRQAHRQVMLIPISAHGTNPASAVMAGMQVVVVKS
                     DEEGHIEISDLKAKAEQYKNVLAGLMVTYPSTHGVFEEGIKEICRLIHENGGLVYMDG
                     ANMNAQVGLTSPGFIGA"
BASE COUNT         1126 a         1125 c         1079 g         1033 t
ORIGIN      
        1 accgaaaaag ggggaacgat cggctattat ctctccagaa aaaatatcgc tgtggtggat
       61 gccgaatttc cggaacaatc caaacacctc atcgacgcgc ttcaaaaaga cccgggaaag
      121 ccatttgaaa ttctgatcaa cacccatcat cacggggacc atacttcggg aaatatttca
      181 ttcaggggga tcgtaaaaca tgttgccgcc cacagcaatt cgctgatcaa ccagcggcgc
      241 gtggcagagg aacaaaaaaa ggaggatcag caactctatc ccgatcttgt gtacggagat
      301 gcctgggagt acaaaaaatt aaaagagatc attaaaacct attatttcgg acccgcgcat
      361 accaacggag acagcatcat tcatttcccg catgccaata ttgcccatat gggcgacctg
      421 gtctttaacc ggcgctgggc atttatcgac cggacggcag gggcttccgt caaaaactgg
      481 attgttgtcc tggacaaggc ccagcagcta tttgacaaag acaccctgtt tatttacgga
      541 catgcttttg atccggataa ggtaaccgga tataaggagg atctgaaagc gatgcaggat
      601 tatctttccc gactgctgga ttttgttgac aaacagatca aggcaggtaa aacaagagaa
      661 gaaatcctga aggccaaagc aatccccggc gtcacggaat ggcagggaga cggaatagag
      721 cgcgggttgg aagctgccta tgatgaacag atacagggaa tcatttaggc tcgtccagca
      781 atccttccag tacggaaacc acgatggggc agaaactgct gttcatctgc gttagcttcg
      841 gtctttttaa gatcacatct tcatcggtat aggggaatct ccggcaatcc gaaggacggt
      901 cttcataaat agaacaccgg ttatcattcc ccaggaaagc gcagggcgtg gaaatcagta
      961 cataatcacc ctcttcatcc agttgcaggt atttttcgat aaaatcgcct tccttcatct
     1021 gaagccttct ggagatcctt ttgatatccg gcgttttaaa acggggggaa taccgtttac
     1081 agcatcgggc gcattgcaaa cagtcaatct gccggaaagc ccgttcatgc aaatcgggca
     1141 gccgttttaa cactttattc ttatttgcct tcgccaggaa atctttatac tttttttgat
     1201 gatctccgga taccttctgc cagttctgca acgttattgt taatggattt tgcattttaa
     1261 acaagttaat taatttcgcc catgaccggc tcactccgag aacaattcct gatactcatc
     1321 agcaccccgc tgtacatctt cattatcggc ctggaattgc tgcttagtca ttttcagaag
     1381 cgcagattat acagctggaa agatacggca agcaatgttt acctgatgct gatgaatgcc
     1441 atcatcgatc tttctttccg ggcaatttac gtggccatcc tggttcaatt attcgatcat
     1501 aaactgattt cctggaaaat ggggataagt tactgggtca tgctgctgct ggccgaggac
     1561 tttttatact actggttaca ccgtttcgat catgaagtgc gcctgttctg ggccgtgcac
     1621 gttacccacc attcttccga atacatgaat ttcacggtcg gattccgttc ttctgttttt
     1681 cagccgctgt accggttcat ctactttatt ccactggcct ggctaggctt caaaccgctg
     1741 gacatcgtat tcatttattc cgccacccag atctggggaa tctttgttca taccgacctc
     1801 atccggaaaa tgggctggct ggaatatttc ctggtaacgc cttcccatca ccgggtacac
     1861 cacgcatcca atccgaaata cctggacaaa aacatgggga tgttcctcat tgtctgggac
     1921 cagctgttcg gaacctttca accggaactg gaagaccagg agtaccagga aaaaagatac
     1981 ggactgacca aaccacttga tagatcttcc cccggaaacc tcgtttttca tgaatggcgg
     2041 gccatccgca aagacctggc ccgcaccgat atttcctgga aggataaatg gtattacgtt
     2101 ttcggcccgc cgggctggag ccacgatggc agccggcaaa ccagccgcca gctcctggaa
     2161 aaggaagaac ttaaggtccc cgaaccggga gaggcctttc aaacccggta agattgatgg
     2221 ttctatatta actttgcgca tctttttgat caatcatcaa ccgtagtatg aatttatttg
     2281 agaaacaggt gaatgagttc gcaaggcgtc atattgggtc caatgaaacg gaaacccggc
     2341 aaatgctggc gcagatcggg gtagaatcac tggatgcact catcaataaa acaattcccc
     2401 aaaccatccg tcaccccctt cccctccagg tgacccctgc tgtgagcgaa tatgaatacc
     2461 tcaaactgct ccgggaaatc gcatcggaaa acaagctgtt caaaaattat atcggacagg
     2521 gttattatga taccataacc cccagtgtca ttctccgcaa tgtatttgag aatccgggat
     2581 ggtatactca atatacccct taccaggcgg agatatcgca gggccgcctg gagagtttgc
     2641 tgaacttcca gaccatcgtg agcgacctga ccggacttcc gatcgccaac gcgtcgctgc
     2701 tggatgaagg gaccgcagca gcagaaggca tgatcatgtt ttttaaccag aagaataagg
     2761 atcatcagcg ggtgaccgtt cccagattct ttgtggacca ggcaatattc atccagaccc
     2821 gggatgtact ggccacccgc gcggaggccc tgggcattga actcgtgacc ggcaattttg
     2881 aacaggtaaa aatagacgcc tcctttttcg gcgcgatcct tcaatacccg gatagcaatg
     2941 gaaacatatc cgatcaccgc gcttttatcc ggaaggttca cgatgccggt gcgtttgtgg
     3001 tcatggcaac ggacctgctg gcgcttacgc tgcttactcc tccgggagaa ctgggtgccg
     3061 acgtggccgt aggttctgcc cagcgcctgg gcgttccggt gggatttggc ggaccgcatg
     3121 ctgctttttt tgcggcagcc gaacaattta aacgggcaat tcccggaagg atcatcgggg
     3181 tgagtgtgga tgcccagggt aaccgggcac tccggatggc gctgcaaacc agagaacagc
     3241 atatcaaacg ggaaaaagcc acttccaaca tatgtacggc ccaggcactg ctggccaaca
     3301 tggccgcgat gtatgcggtt tatcacggtc cggccggact gaaagaaata gctacccgca
     3361 tctgcgtcct ggcccagggt ttatccgaag ggctcgcccg cctgggattc gaaaacgggc
     3421 acaatcattt ctttgatacc gtccaggtcc ggtcggataa tacagaacaa ctccgggaga
     3481 ttgcggaatc ccggcagatc aatttttttt acggggaaag cgggaaagta acgatctccc
     3541 tgaatgaaag cagcacacag gccgatgtgc tggacatcct gtcggtattt gcccagtcaa
     3601 gggatattga cgaggccgct accatttttc aatacgacgg ccatctggag agtataccgg
     3661 ccacccttaa ccgaagttcg gcatttctga cccatcccgt ttttaatgcg caccacagcg
     3721 aaacggaaat gatgcgttat ctgaaaagcc tggagaacag ggacctttcc ctgaacagtt
     3781 ccatgatccc gctcggttcc tgcaccatga aactcaacgc agccagcgaa ctcattcccg
     3841 tcagctggcc cgaattcagc cggatacatc cttttgcccc acccgatcag tggaagggct
     3901 atcagcggat catatcggag ttggaagaat ggctgagcat catcaccggt tttgccgcca
     3961 cctctcttca gcccaacagc ggggcgcagg gagaatatgc cggattgctc gctatccggt
     4021 cttatcacgc agcgcatcgg caggcgcacc gccaggttat gctgatcccg atttccgccc
     4081 acggtaccaa tccggccagc gcggtaatgg ccggcatgca ggtggtcgtg gtaaaaagcg
     4141 atgaagaagg ccatattgaa atctccgatc tgaaggcgaa ggccgaacaa tataaaaatg
     4201 tactcgccgg gctgatggtt acctatcctt ccacgcacgg cgtttttgaa gaagggataa
     4261 aagagatctg ccggttgatc cacgagaatg gagggcttgt ttacatggac ggggcgaata
     4321 tgaatgccca ggtcggattg accagtccgg gttttatcgg ggc
//
LOCUS       DASYCU010000154         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1634465, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000154 DASYCU010000000
VERSION     DASYCU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634465"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..1244
                     /locus_tag="VGM24_03060"
     CDS             417..1244
                     /locus_tag="VGM24_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4154370.1"
                     /translation="MKLDIHSDVLVWFGHSSYFIQLDGKRILVDPVFSGSASPFSFSV
                     KAFEGTDIYTTDDIPVIDYLFISHDHWDHLDYETIIKLKPRVKKVICGLGTGEHLEYW
                     GYHQDLIIEKDWDGQIDPGDGFQVHAVPARHFSGRGLIRNKAIWTAFVLQTPTMQILI
                     GGDSGYDKHFAAIGEQFGPFDLVILENGQYDKSWKYIHMMPEEVLQAAEDLNAKRLLP
                     VHSCKFAIANHAWDDPLKKITELGKTRDVAIITPRIGEAVYLKDPAQVFMEWWKNIK"
     gene            complement(1265..2020)
                     /locus_tag="VGM24_03065"
     CDS             complement(1265..2020)
                     /locus_tag="VGM24_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4154371.1"
                     /translation="MKFLFLTLLFTPVLFSGYAQRSNPDILQTKMGPLVIQPIEHASL
                     ILTVHGLTIYADPSNPDNYKGLTPPDIILITDIHGDHFDIKTIDAIKKTNTLLVVPQV
                     VADKLPAADKDHVVVLKNGGQTMQSGVSILAIPMYNLPESPTAMHTKGRGNGYVLGIG
                     GKLIYLSGDTQGIPEMRSLKNIDVAFVCMNLPYTMDIKEAADAVLAFKPKIVYPYHYR
                     GQNGLSDVNAFKDLVAAGNKKIEVRLRNWYPGK"
     gene            complement(2057..>2861)
                     /locus_tag="VGM24_03070"
     CDS             complement(2057..>2861)
                     /locus_tag="VGM24_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEY4154372.1"
                     /translation="SFLCLNGFGQQIKIPSPSPVQNIKQEFGLSTIELSYSRPGVKSR
                     KVFGDLVPYGKLWRTGANQATTLSFGDSVIIGGVAIPPGKYGLLTIPGSDEWTIIITK
                     QLDVTSPSAYKADQDIVRVKARTQEFPYPIETFTILFSNIRSGSCDLAMIWDRTLVAL
                     PIKTETDSRVMAQINAAMTNDSFPYFTAALYYLDNKKDLYKALEWFNKAVDQNPKAYF
                     MFYQKARCQAKMGKNKEALATAQQSMKLAKEAKNDDYVQLNEKLIATLK"
BASE COUNT          714 a          664 c          721 g          762 t
ORIGIN      
        1 atataacgcg gctatgccac ggtcccgaaa acccgaaatg cggctgggcc gccggacgga
       61 aaaattcaat ccgggactgc gggcgtatcc tcggcaaatg cagctggcga taatgtctat
      121 ctttaaattg caaaagataa gcacccgtca tgtatctaat tatattcctg atcattctag
      181 ccttaacggc cgttgttttt ttccggcaaa ggaaattcgg caggttgccc accggtgccc
      241 ggttagaaaa gatcagggag tcgccgaatt atagagatgg agcttttcaa aatcaaagcc
      301 cgactccgga ccttaccgaa ggagccggtt atttttccgt attaagagag tatgtttttg
      361 gaaaggataa acgggttcgg ccccgggaca gcatcccctc aatcaaaacg gatcttttga
      421 agctggacat acattccgat gttttggtct ggttcggaca ttcctcttat tttatccagc
      481 ttgacggaaa gcgcatcctg gtcgacccgg tattcagcgg ttcggcctcc cctttttcat
      541 tctccgttaa agcgtttgaa ggaacagata tttatacgac cgacgatata ccggtgattg
      601 attatctttt tatttcccat gaccactggg atcatctgga ctatgaaaca ataatcaaac
      661 tgaaaccgcg cgttaaaaaa gtcatctgcg gtctggggac cggagagcat cttgaatact
      721 ggggttatca tcaggacctc atcatagaaa aagactggga cgggcaaatc gatccgggcg
      781 acggttttca ggttcatgcc gttccggcgc ggcatttttc cggccgcggc ctcatccgga
      841 ataaggcaat ctggacagct ttcgttttac agacacccac gatgcagatc ctgatcggag
      901 gcgatagcgg gtatgacaaa catttcgccg ccatcggcga acaatttggc ccctttgatc
      961 tggttatcct ggaaaacggg caatatgata aaagctggaa atacatccac atgatgccgg
     1021 aagaagtcct gcaggcggcg gaggacctga acgcaaaacg gcttttgccg gtgcactcct
     1081 gcaaatttgc cattgcaaat catgcctggg acgacccatt aaaaaaaatt acagaactcg
     1141 gaaaaacgag ggatgtcgcc atcatcacgc cgcgcatcgg cgaagcggtc tacctcaaag
     1201 acccggcgca ggtctttatg gaatggtgga aaaacattaa atgatacgtt aaccgcggac
     1261 cctgctattt tcccggatac cagtttctta accggacttc gattttttta tttcctgctg
     1321 ccacgaggtc tttaaaagca ttgacatcgc tcaagccgtt ttgtccgcgg taatggtagg
     1381 gataaacaat tttaggttta aatgccagaa ccgcgtccgc cgcctcttta atatccatgg
     1441 tataaggcag gttcatgcac acgaaggcca cgtctatatt ttttagggac cgcatttccg
     1501 gaattccctg tgtgtctccg gaaagataaa tgagcttgcc gcctatgcca agcacatacc
     1561 cattgcctcg gcctttcgta tgcatggccg tcgggctctc gggcaggtta tacatgggaa
     1621 tggcaaggat ggaaacgccg gattgcatag tctggccgcc gttcttcagc acaaccacgt
     1681 gatccttgtc agcggcaggc aatttgtctg ctaccacctg aggcactaca agcagggtgt
     1741 tggttttctt aattgcatcg atggttttta tatcaaagtg gtcgccatgt atatcggtaa
     1801 taaggataat atccggaggc gtcaggcctt tataattatc cggattcgaa ggatcggcat
     1861 agatggtcaa accatgcacg gtgagtatca ggctggcatg ttcaatgggt tgtatgacaa
     1921 gcggacccat ttttgtttgc aggatgtccg gattgctgcg ttgggcatat ccggaaaaca
     1981 atacgggggt aaacaacagt gtaagaaaaa ggaatttcat aacagatgaa ttagatacgg
     2041 taaatatact gctcatttat tttaaagtgg caatcagctt ttcattcagc tgcacataat
     2101 cgtcattttt tgcttctttg gcaagcttca ttgattgctg agcggttgcc agcgcttctt
     2161 tgttcttgcc cattttcgcc tggcaacggg ctttctgata aaacatgaaa taggcttttg
     2221 gattctgatc aactgcctta ttaaaccatt ccagcgcttt gtacaggtct tttttgttat
     2281 ccaggtaata cagagcagcg gtgaaatagg ggaagctgtc gttggtcatg gccgcgttga
     2341 tctgcgccat aaccctggaa tctgtttctg ttttgatggg cagcgcaacc agtgtccggt
     2401 cccagatcat tgcgaggtcg cagctgccgg acctgatgtt gctgaacaaa atggtgaagg
     2461 tttcgatggg gtaagggaat tcctgtgttc tggctttcac ccggacaata tcctggtcgg
     2521 ccttataagc ggaaggactg gttacatcca gttgtttggt aatgatgatg gtccactcgt
     2581 cggatcccgg aatggtcagt aatccatatt tgccgggagg tattgccacg ccgccaatga
     2641 tcactgaatc tccgaaactg agtgtggtgg cctggttggc gcccgtccgc cacagttttc
     2701 catagggcac caggtcgcca aagaccttcc tgctttttac gcccggtctg gaataggaaa
     2761 gctcgatcgt ggaaaggccg aattcctgtt ttatgttctg gacaggagaa ggagagggta
     2821 tctttatttg ctggccaaag ccattcaggc ataggaaaga a
//
LOCUS       DASYCU010000155         6969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2792904, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000155 DASYCU010000000
VERSION     DASYCU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2792904"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2719)
                     /locus_tag="VGM24_03075"
     CDS             complement(<1..2719)
                     /locus_tag="VGM24_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4154373.1"
                     /translation="MFKRFIERPVLSTVISIILTLLGLISMFSLPITQFPDIAPPTVN
                     VSAVYPGANAEVVARAVATPIEEAINGVENMTYMTSSSSNDGSMSLNVYFQLGTNPDL
                     AAVNVQNRVSKAVSQIPQEVVQAGISTQKQQNSMIMVPLLYSTDTAYDEKFLQNYAKI
                     NIIPEIQRVPGVGQAQVFGAKDYSMRIWLKPDRLNADNIGVQDVLNAIHDQNVEAAPG
                     RFGEASTASFEYILKYKGKFSQKTDYENIIIKAGADGSLIRLKDVARVEFGSFAYSSN
                     SLIQGKPGTGIAIFQTAGSNANDILIAVDKVMKKAATSFPRGVHYFTMFSAKDFLDAS
                     ISQVEETLLEAFILVFIVVFLFLQDFRSTLIPAIAVPVAIVGTFFFMQLFGFTINLLT
                     LFALVLAIGIVVDDAIVVVEAVHSKMERTGLGARESTISSMNEISGVIISITLVMAAV
                     FVPVGFMQGPAGVFYRQFAFTLATAILISALNALTLSPALCALFLKNNNHETDGRKRG
                     FGKRFGDAFNAGFKSTTLRYSHAVHFLIRRKWIALLGLAAVILATVWMMNTTPSGFIP
                     TEDQNFVLLSLNLPPGASLDRTEAVTKKVDSLLNEVQGVDRRGIVTGLNILANATSSA
                     YGVGFIKLKPQKERVGAKSIEEVMAAINQKLSVIKEASIFVFTVPTVQGFGNTSGFEF
                     IVQDRTGGSLEKLGSVANGFIGDLMKRKEIAYAFTTFNTSNPQYLLDVDDAKAKQLGV
                     SVSDILGTLQVYYGSSFASDFNRFGKYYRVIVQADVPYRTGPASLDGIYVKNDQGRMV
                     PVSTLVQLQRVYGPETVNRTNLFNSVTINGTVKPGFSSGDAIKAIQEVGEQYLPRGYS
                     YEWSGMTREEKGATSQTSLILILSVVFVYFLLSAQYESYILPFAVLLSIP"
     gene            complement(2759..3916)
                     /locus_tag="VGM24_03080"
     CDS             complement(2759..3916)
                     /locus_tag="VGM24_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4154374.1"
                     /translation="MRNYLFSGALVLFVFTALESCHNSEGNTPYAPAVVSLPVITLSN
                     QPFTSYQEYSASLEGNKDVEIRPQVNGYLDKIFVDEGAQVKKGQPLFQINDRPYREAL
                     NNAKASWTATKANLASAEINVNKLTPLVQNKVISPVQLKSAQAVYDAAAASVAQAQAQ
                     VGNAEINIGYSLIRAPMDGFIGRIPFKTGSLVGMTTTEPLTTLSEIKEMRAYFSLSET
                     DFLQFKKQYPGNTIAEKLKQVPPVELILADNSVYPEKGKVEIVSGQFANGTGSIPFRA
                     SFPNAAGTLRSGNTGRIRITTVVPSGLVIPQESTFELQDKVFVFVLGDSNKVRSVPVN
                     ISGRSGNYYLVDQGVKAGDRIVYTGVDRLRDGAMIRPENMALDSLLKVRPM"
     gene            complement(4001..4603)
                     /locus_tag="VGM24_03085"
     CDS             complement(4001..4603)
                     /locus_tag="VGM24_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4154375.1"
                     /translation="MGIAERKLRRREEVRNLILDTAWQMVKKEGWRALSLRKISDAIE
                     YSVPVIYDHFENKEAILLELSRQGFALLTKKLSQAKEKQRNPALQLQGIADAYWNFAF
                     RNKEYYQLMFGLGMPSCDIDKSVPEKNSFRNLVMEPILSMIKANGKKNVDACLKYHTF
                     WSVLHGLVSIKMLGSTNALDTLNKLVLEDAVSGFIRNLEN"
     gene            complement(4701..6176)
                     /locus_tag="VGM24_03090"
     CDS             complement(4701..6176)
                     /locus_tag="VGM24_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HEY4154376.1"
                     /translation="MNKMIFPATLSVLLAFSTLPSGLPYDNRIREGKPALLASKILVY
                     TTAEKTDWRLSLTDSLSFSDFGQPLETQPCIFIDPDKKFQTFLGIGGALTDASAETFF
                     KLPKAKQAEILKKYFDPGAGIGYTLARTNIQSCDFSSDSYSYVKDGDADLKSFSLAHD
                     EAYRIPFIKQAMAAAGGKLTVFATPWSPPAWMKDNHDMLHGGKLLPAFDQAWANFFVA
                     FIRGYEKEGIPVWGLSTQNEPMAKQTWESCIYTGTEERDFIKNFLGPTLKQAGLGDKK
                     IIAWDHNRDLLYQRASTVLDDPEAAKYVWGVGFHWYESWTGGGKIFDNVRRVSEAYPS
                     TNLIFTEGCAEKFNASQIADWKWGEDYGRNMIEDFNNGTVGWMDWNILLDEKGGPNHV
                     GNFCFAPVHANTRTGEITYMNSFYYIGHFSKFIKPGAKRIISSSSRGQLLTTAFQNPD
                     GSVATVVMNQSGEKFSYHLWLKGSAAEITIPPHSIQTLVLE"
     gene            complement(6226..>6969)
                     /locus_tag="VGM24_03095"
     CDS             complement(6226..>6969)
                     /locus_tag="VGM24_03095"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY4154377.1"
                     /translation="LEAHIKIWEDVSVPDSKKPVGFILSLEGADSLVNLSYLKKAHAY
                     GLRALGPAHYGEGRYAGGTGREEGFSTEGKKLLKEMQSLNIILDVTHLTDKGFTEAMD
                     IFHGPVWASHHNCRALVKNQRQLTDEQIKILIDKGAVIGGVMDAWMLTDGWIRGVSLP
                     KATGTDLNRLINHYDHICQIAGNCDHVAIGSDLDGMFGTEQSPYDMDTIADLRKLPEL
                     LKARGYAAADIEKLLHGNWLRFLRKAWKA"
BASE COUNT         1641 a         1816 c         1727 g         1785 t
ORIGIN      
        1 taggtatgga taacaatacg gcaaagggaa gaatataact ttcgtattgg gcggataata
       61 agaaatagac aaacacgaca ctgagaatca atatgagact ggtctggctg gtcgctcctt
      121 tctcctcccg ggtcatgccg gaccactcat aggaataacc gcgcggcaga tattgttcac
      181 ccacttcctg tatggcttta atggcgtccc cggaactgaa tcccggtttt acggtgccgt
      241 tgatggtaac ggaattgaac aggttggtcc ggtttaccgt ttccgggcca tacacccgct
      301 gcagctgaac cagggtactc accggaacca tccggccctg gtcattcttt acatagatcc
      361 catcgagtga agccgggccg gtccggtagg gaacatctgc ctgaacgatc acccggtaat
      421 atttaccaaa gcggttaaag tcggatgcaa aactgcttcc ataataaacc tgcagggttc
      481 ccaggatatc actaacggac acgcccaatt gctttgcttt ggcgtcgtcc acatccagca
      541 ggtattgcgg attggaggta ttgaacgtgg tgaaggcata agcaatttcc tttctcttca
      601 tcaggtctcc gatgaagcca ttggcgacag atccgagttt ctccagggat ccgccggtgc
      661 gatcctggac aatgaattca aaaccactgg tatttccgaa tccctgtacg gtgggaacgg
      721 tgaacacaaa gatgctcgcc tctttaatga ccgagagttt ctgattgatg gcggccatta
      781 cctcttctat gctttttgca cccacccttt ccttctgggg cttgagcttg atgaatccaa
      841 ccccgtaagc ggagctggtc gcgttggcca ggatattcaa tccggtcacg attcctctgc
      901 ggtcaacccc ctgcacttca ttgagcaggc tgtccacttt ttttgtaacc gcttcggtcc
      961 ggtcgaggga cgctcccggc ggcaggttaa gggagaggag tacgaaattc tggtcttccg
     1021 tgggaataaa accggaaggc gtggtgttca tcatccaaac cgtagccagg ataactgcgg
     1081 ccagaccgag gagggcgatc catttccggc ggatcaggaa atgtacggca tgggaatacc
     1141 gcagggtggt ggatttaaac cccgcattaa atgcgtcgcc aaaccttttt ccaaatcctc
     1201 ttttccggcc atccgtttca tgattgttat tctttaagaa caaggcgcaa agcgccgggc
     1261 tcagggtaag ggcgttcagt gcggaaatga ggatggccgt agcgagcgta aaggcaaact
     1321 gccggtaaaa taccccggca ggaccttgca tgaatccgac gggaacaaat acggcggcca
     1381 tcaccagggt gattgaaatg atcactcccg agatctcatt catggaactg atggttgatt
     1441 ctctggctcc caatcccgtt cgctccattt ttgaatgaac ggcttccacc accacgatgg
     1501 catcatccac gacaatacca atggccagca cgagagcgaa aagcgtcagc aggttgatgg
     1561 taaacccgaa taattgcatg aagaagaaag taccgacgat ggccaccggc acggcaatcg
     1621 ccggtatcag cgtggaccgg aaatcctgca ggaaaagaaa taccacgatg aataccagga
     1681 tgaacgcttc caggagggtt tcctccacct ggctgatgga agcatccaga aaatcctttg
     1741 cactgaacat ggtaaaataa tgcactcctc tcggaaaact ggtcgctgct tttttcatca
     1801 ccttgtccac ggcaatcaga atatcattcg cattggatcc ggcggtctgg aaaatggcta
     1861 tcccggtacc cggttttccc tggatcagtg aattggagct gtaagcgaag gatccgaatt
     1921 ccacccgggc aacgtctttc aggcggatca gggagccgtc ggcgcccgcc ttgatgatga
     1981 tgttttcata atctgttttt tgggagaatt tccccttgta tttaagaatg tactcgaaag
     2041 acgcggtgct ggcctccccg aatcttcccg gagccgcttc cacgttctgg tcatgaatgg
     2101 cgttcaatac gtcctgcacg ccgatattgt ccgcattcag gcggtccggt ttcagccaga
     2161 tgcgcatgga ataatctttt gcgccgaaca cctgggcctg cccgaccccc gggacgcgct
     2221 ggatctccgg gatgatattt attttggcat agttctgtaa gaatttttca tcatacgccg
     2281 tatcggtgct gtaaagcagg ggcaccatga tcatgctgtt ctgctgcttt tgcgtcgaga
     2341 taccggcctg taccacttcc tgcgggatct ggctaacggc cttggatacg cggttctgca
     2401 cattcacagc cgccaggtcg gggttggtgc ccagttgaaa atatacattg agcgacatgc
     2461 ttccgtcatt gctggaagaa gacgtcatat aggtcatgtt ctctacgcca ttaatggcct
     2521 cttcgatggg agtggccacc gcgcgggcca ccacttcggc attggctccc ggatacaccg
     2581 cggatacatt caccgttggc ggcgcaatat ccgggaactg ggtgatgggc agggagaaca
     2641 tcgatatcaa tcccagcagg gtaagtatga tggagatcac ggtagacaat accggtcttt
     2701 caataaatct tttaaacatc agaaaaaatt tgtcggtaat gaatggaaag gctgcggttt
     2761 acatgggtct cactttgagc agactatcca aagccatgtt ctccggccgg atcatggctc
     2821 cgtcccggag ccggtccacc ccggtataaa caatccggtc gccggctttc acgccctggt
     2881 caaccagata gtagttcccc gatcttccgg aaatattcac cggcacgctg cggaccttgt
     2941 tgctgtcgcc caacacaaaa acaaacactt tatcctgcag ttcgaaagtg gattcctggg
     3001 ggatcaccaa gccggaaggc accacggtgg taatgcggat cctgccggta tttcccgacc
     3061 ggagcgtgcc cgctgcattc ggaaagctgg cccggaaagg aatggatccg gtgccgttgg
     3121 caaactgacc ggaaacaatt tccacctttc ctttctccgg ataaacggaa ttatcggcaa
     3181 ggatcagttc caccggagga acctgcttta atttttcagc tatcgtgttt cccgggtatt
     3241 gctttttgaa ttgcaggaaa tccgtttcgc ttagggagaa ataagcccgc atttctttta
     3301 tttccgaaag ggtggtcagc ggctccgtgg tggtcattcc aaccaggctt cccgttttaa
     3361 acggaattct tccgataaat ccgtccatcg gcgccctgat caggctgtac ccgatattga
     3421 tttccgcgtt gcccacctgc gcctgcgcct gcgccacgct ggcggctgcc gcgtcataca
     3481 cggcctgggc ggatttcagc tgaaccggtg aaatcacctt gttctgcacc agaggggtga
     3541 gtttatttac attgatctcc gcactggcca ggttggcctt ggtagccgtc caggaagctt
     3601 tcgcattatt caatgcttcc cggtacgggc ggtcgttgat ctggaataag ggctgccctt
     3661 ttttaacctg tgctccctca tccacaaata tcttatccag ataaccattc acctgggggc
     3721 ggatctccac gtccttattg ccttccagtg aagcactgta ttcctggtaa ctggtaaagg
     3781 gctgattgga caacgtgatc accggaaggg aaaccaccgc gggtgcgtag ggcgtgtttc
     3841 cttctgaatt atgacaggat tccaaagccg tgaatacaaa taaaaccagg gcgcctgaaa
     3901 acaggtaatt tctcataatt atactttgac gggagttttg cactccgggt taaacagaat
     3961 aaatgttttt gatggctttc gttagtgggg attcaggacg ttaattttcc aggtttctga
     4021 taaatccgga taccgcatct tccagcacga gtttattcag ggtgtccagc gcatttgtac
     4081 tgcccagcat tttaatagat accaatccgt gcaatacaga ccagaacgta tgatatttca
     4141 aacaggcatc cacgtttttt ttcccatttg cctttatcat ggacaggatg ggctccatca
     4201 ccagattccg gaacgagttt ttttccggga cagatttgtc aatatcacag gagggcattc
     4261 ccaatccgaa catcagctgg tagtattcct tgttccggaa ggcaaaattc cagtaagcgt
     4321 ccgcaatccc ctgcaattgc aatgccggat tcctttgctt ttcttttgcc tgacttaatt
     4381 ttttcgtaag cagcgcaaag ccctgcctgg acaattccag caggatggcc tccttgtttt
     4441 caaaatgatc ataaatcacc ggcacgctgt attcgatggc atccgagatc ttacggagcg
     4501 ataaagcacg ccagccttcc ttcttcacca tttgccaggc ggtatccaga atcagattcc
     4561 ggacctcctc cctgcggcgt aattttcttt ccgcgatacc catattacgg attttcttaa
     4621 caccgttagc aaaagtagca ctgtttggca aagcgccaaa aacaaatgcg ggggaaaggc
     4681 aatatttttt ttgagcggtc ttattccagc accagggttt gaatggaatg cggcggaatg
     4741 gttatttccg ctgccgaccc tttcagccac agatgatagc tgaacttttc gccgctctgg
     4801 ttcatcacta cggttgccac ggaaccatcc gggttctgga aggcagtcgt gagtaattgt
     4861 ccccggctgg aagaactgat gatgcgtttt gcccccggtt taataaattt tgaaaaatgg
     4921 ccgatataat agaaggagtt catgtacgtg atctctccgg tccgcgtatt ggcgtgaacg
     4981 ggagcaaagc agaaattacc gacatggttc ggaccccctt tttcatccag caatatattc
     5041 cagtccatcc atcccaccgt accgttatta aaatcctcga tcatatttct tccataatcc
     5101 tccccccact tccagtccgc gatctgggac gcattgaatt tttccgcaca gccttcggta
     5161 aaaataagat tggtagaagg ataggcttcc gacacccgcc gtacattgtc aaatattttt
     5221 cctccgcccg tccagctttc gtaccagtga aaacccacgc cccatacgta tttggccgct
     5281 tccggatcat ccagcacggt gctggcgcgc tgatacagca gatcgcggtt atggtcccag
     5341 gcgatgattt ttttatcccc gaggccggct tgtttcaggg tgggcccaag aaaattctta
     5401 ataaaatcac gctcttccgt tccggtataa atacaggatt cccaggtctg tttagccatg
     5461 ggttcgttct gggtggacag tccccatacc ggtatcccct ccttttcata tcccctgata
     5521 aaggcaacaa aaaaatttgc ccaggcctgg tcaaaagccg gcagcagctt tccgccatga
     5581 agcatgtcat ggttgtcttt catccaggcc ggcgggctcc agggcgtggc gaacaccgtg
     5641 agctttccgc ctgcagcggc catggcctgt ttgataaagg gaatgcggta cgcctcatca
     5701 tgtgccaggg aaaaggattt caggtctgca tctccatcct ttacataact ataggaatcg
     5761 ctggagaaat cgcagctttg gatattggtg cgggcaaggg tgtatccgat tccggccccg
     5821 ggatcgaaat attttttcag gatctcggcc tgttttgctt tgggaagttt aaaaaatgtt
     5881 tctgcagaag catccgtcaa tgcgccgccg atgcccagga aggtttgaaa ttttttatcc
     5941 ggatcaatga atatgcaggg ctgggtttcc agcggttggc cgaaatcgga aaaggagagg
     6001 gaatcggtga gggaaagtcg ccagtccgtt ttttctgccg tcgtatatac caggatctta
     6061 gatgccaaaa gagcaggttt cccttcgcga atgcggttat cgtacggcag gccggaggga
     6121 agggtggaaa aggcgagcag taccgacagc gtggcgggga aaatcatttt attcatatga
     6181 ccggttttaa gggaattaaa gatagacgat cgttcccatt tcccgctagg ctttccatgc
     6241 tttcctcaaa aaccgcagcc agtttccgtg taataatttt tcaatatccg ctgcggcata
     6301 ccctcttgcc ttcagcaatt ccggaagttt ccggagatcg gcgatggtat ccatatcata
     6361 aggcgactgc tccgtaccga acattccgtc cagatcgctg ccgatcgcaa catgatcaca
     6421 gttcccggcg atctggcaaa tatgatcgta gtggttgatc agccggttca ggtcggtacc
     6481 cgtcgccttg ggaagcgaaa cgccccggat ccagccatcg gtgagcatcc aggcatccat
     6541 cactccgcca atgaccgctc ctttatcgat cagtatctta atttgttcat cggtgagttg
     6601 cctttgattt tttaccagcg cccggcaatt gtgatggctg gcccagaccg ggccatgaaa
     6661 aatatccatc gcctctgtaa atcctttgtc cgtcagatgg gttacatcga ggatgatatt
     6721 cagggattgc atttccttca gtaacttttt tccctccgtg ctgaaccctt cctcccttcc
     6781 tgtacccccg gcataacggc cttccccgta atgggcagga cccaatgcac gcaaaccata
     6841 ggcatgtgct tttttgagat aggaaaggtt aacgagggaa tcggcccctt ccaggctcag
     6901 aataaagccg accggttttt tactatcggg aacagataca tcttcccaga ttttgatatg
     6961 cgcttccag
//
LOCUS       DASYCU010000156         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1778182, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000156 DASYCU010000000
VERSION     DASYCU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778182"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VGM24_03100"
     CDS             <1..163
                     /locus_tag="VGM24_03100"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY4154378.1"
                     /translation="GYEYSHSYEKNFFPQEYLPGEISGNVFYEPGKNAREEETRKHLR
                     SLWKEKYKY"
     gene            complement(205..1689)
                     /locus_tag="VGM24_03105"
     CDS             complement(205..1689)
                     /locus_tag="VGM24_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4154379.1"
                     /translation="MDICIYGSTSGGVIAAYTAARLGKRVLLVTPDRQPGGITSGGLG
                     NTDIGNKQAITGMARDFYRRVGKHYGKPEQWVFEPHVAEGIFREYLRLAKVEVWYSQE
                     LLSVIRNQRKIREIRVGNTGSGPAAGERIVKAKVFIDCSYEGDLMAKAGISYTVGRES
                     NAQYHEMYNGVELLDKHQFPDGVDPYLVPGNPQSGLLWGISGEALAPKGSGDKKIQAY
                     NFRICLTDSAANRIEISRPESYDSGRYTLLLRYLEKKNPASLEDRVLNINLMPNRKTD
                     INNNGPFSTDLIGMNYAYPEGDRETRKKIVQKHESYAKGLLYFLGHDMRVPEHLRKEM
                     LQWGYPKDEYPANGNWSPQLYIREARRMIGAYVMTEANCTGKERVSDGIGLAAYTMDS
                     HNCERLVVNGMVKNEGDVQIGGFPPYPVSYRALIPKADECTNLLVPVCLSASHIAYGS
                     IRMEPVFMVLGQSAAVAAVLALNRQLGVQQVRAGDIQEWNKRIR"
     gene            1922..>2133
                     /locus_tag="VGM24_03110"
     CDS             1922..>2133
                     /locus_tag="VGM24_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4154380.1"
                     /translation="MICWTCKKFEDLSPHELYAVLRLRNEVFIVEQHCPYPDCDNKDF
                     KAWHLMGAENENLLAYSRLIPAGISYP"
BASE COUNT          496 a          569 c          513 g          555 t
ORIGIN      
        1 aggctatgag tacagtcata gttatgaaaa gaattttttt ccgcaggaat atcttcccgg
       61 ggagatcagc ggtaatgttt tttatgagcc cggtaaaaac gcaagggaag aggagacgcg
      121 taaacacctg agaagcctct ggaaagaaaa atacaagtac tgatgcgcgg cagaaacagc
      181 tgatgcggat caggcatttt ccaattatct gatccgtttg ttccactcct gtatatcgcc
      241 cgcccgcacc tgctgaaccc ccagctgtct gttcaatgcc agtactgccg caacggcggc
      301 cgactggccc aggaccataa ataccggttc catccggata gaaccatagg caatatggct
      361 ggcggacagg cataccggca ccaggaggtt tgtacattca tccgctttcg gaataagggc
      421 gcggtaagaa acaggatatg gaggaaatcc gccaatctgc acatcccctt catttttcac
      481 cattccgttc acgaccagac gctcgcaatt gtgggaatcc atggtatagg ctgccagtcc
      541 gattccgtca ctgacccgtt ctttgcccgt acaatttgcc tcggtcatca cgtaggcgcc
      601 gatcatccgc ctcgcttctc ggatatacaa ttgcggcgac cagttcccgt ttgcggggta
      661 ctcatctttc gggtaacccc actgaagcat ttcctttctc aggtgctccg gtacgcgcat
      721 atcgtgcccc aggaaataga gcaggccttt cgcataggac tcatgcttct gaacgatttt
      781 cttccgggtt tcccggtcgc cttccggata agcatagttc atgccgatca gatcggtgga
      841 gaacgggccg ttattattga tatccgtttt tctgttgggc atcagattaa tattcaacac
      901 ccggtcttcc agcgaagccg gatttttctt ttcgagatag cgcaacagta aagtataccg
      961 cccggaatca taggactccg gtctgctgat ctcgatccgg tttgcggcac tgtcggtcag
     1021 gcaaatcctg aaattgtatg cctggatctt tttgtcgccg gatccttttg gcgcaagggc
     1081 ttccccgctg atcccccaca acaggccgga ttgcgggttc ccgggaacaa gataaggatc
     1141 caccccgtcg gggaactggt gtttgtcaag cagttcaacc ccgttataca tttcgtgata
     1201 ctgcgcgtta gactcacggc caacggtata cgaaatcccc gctttcgcca tcagatcccc
     1261 ttcgtaagaa caatcgatga ataccttggc ttttacgatc cgctcaccgg cagcagggcc
     1321 cgatccggta ttcccgaccc ggatttcccg gattttgcgt tgattccgga taacggataa
     1381 taattcctgc gaataccaga cctctacttt ggcgaggcgc agatattccc ggaatatacc
     1441 ttcggccacg tgcggttcaa aaacccactg ttccggtttc ccataatgtt ttcccacgcg
     1501 acggtagaaa tcccttgcca ttccggttat cgcctgttta tttccgatat ccgtgtttcc
     1561 gaggcctccc gaagttatgc ctcccggttg cctgtctggc gtgaccagaa gaactctttt
     1621 acccaatctg gcggcggtat aggctgctat cactccgccg gaggtggagc cataaataca
     1681 aatgtccacg gccctggttt tctgcgcggc ggccggggtg aaaagaaaca gggttgaaat
     1741 taaaaaaaga agcaggcgca tgcgttatgg agcattgagt gcttaaagtt atgggtaaac
     1801 ccgaatattg attttaccga tccccctgaa attccgaaat tagccgctgt cgatgggttg
     1861 tttcggcccg cccggtattt tttattccat tttcaaatgg cctgatcatc aaattatcca
     1921 attgatctgc tggacctgca aaaaattcga agacctcagc ccgcacgaac tgtatgccgt
     1981 tttacgattg cgcaatgaag tttttattgt ggagcagcac tgtccttatc cggattgcga
     2041 taacaaggat tttaaagcct ggcatttaat gggggcggaa aatgaaaacc tgttggctta
     2101 ttcccgcctg atccctgccg gaatttctta tcc
//
LOCUS       DASYCU010000157         5575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4389365, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000157 DASYCU010000000
VERSION     DASYCU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4389365"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="VGM24_03115"
     CDS             complement(<1..1634)
                     /locus_tag="VGM24_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154381.1"
                     /translation="MIRPAIVPAILFLGLFSCRIPKDFQPGKPFVYRTNIKVEGKMKN
                     DQRQDLILRLENQLDDSLQVKTITAFDWPWHSHIIYKKLSHPPVYDSLNLGRSIAFMT
                     SLLNANGYYSPVIEDTVKIKTVHKGKVYSRKKYRGQSREEQRVTIDFKVKPGKQLVFD
                     SVGYALRSPELQKLTLDSRSQSVIKKGQPYSRQVLVTEINRLVDTFRNHGYYHYTKDD
                     LYVEHDTVFAALIDPSLDPLEQAELLEKLRLKNENPTINVTVKQRDIRDTTHLLKYYI
                     GKVTVYPDLPVIEDTIAVKTDTTVIRNLQFVTRSNKFKLPFLANNIFLRPGRLYKQEN
                     YYRTFNRINQFSAWQYNNIEFLNSPVADSLLDVNLRMYPAKKQKLTVDLEASRNTNDI
                     VSTSNLFGTGINFGLQNRNAYKQSVQTNTNLRGGVEFGSSFIQTTLASLSHTISIPRI
                     ISPFPIRREGRLRMAQTVINVNASYTDRRAFFKLRSINGSFGYQWSRSRQHTEGELNV
                     NSTRTFLWKPLNIEYTGLNTTDSLDKMLLANPSLKLA"
     gene            1685..2425
                     /locus_tag="VGM24_03120"
     CDS             1685..2425
                     /locus_tag="VGM24_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY4154382.1"
                     /translation="MLVKSQVKYIQSLGHKKFRDAEGVFVAEGPRMVQELLHCEFTAL
                     VKAYATRDWLLENASLIPDKNSITEVDQQELERISFLSAAVSVLAVFRKPVFPVMDKP
                     EGITLLLDNIQDPGNLGTIIRTADWFGVRRIVCSPDTADLFSPKVVQSTMGSITRVEV
                     LYQDLTEYLDAHPGIPAYAAMLDGEDLEQLQRIPEGMILIGNESRGVRPELAERAGRR
                     IRIPGRGSAESLNVAVATGILLFMIQKS"
     gene            complement(2422..3654)
                     /locus_tag="VGM24_03125"
     CDS             complement(2422..3654)
                     /locus_tag="VGM24_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4154383.1"
                     /translation="MNLSSFIARRVAFNRQHSFSRFIIRLAVAATAISVMAMILTIAF
                     TQGFQDAIAQKMYSFSGHIRVQHYEPERVDIAEDLPITANDTVLQTIRADPEVLTVQP
                     FATKYAIIKGPESFEGLRLKGVGRNYRFQHLQAFLKSGHWIRFPDTAYSSEIDLSSYT
                     ADQLNLHVGDKLFAYFIQPDRSRRVRPLKVAGIFKTGIEDYDRINAIVDIRLIQRLNN
                     WTANEISGYELFIKDYQMADSISNRIYYKLPPGWNSLSVSEIYGNIFDWLNLQDTTIA
                     IVIIIMILVAILNLVTCLIILLLERTNMIGLLKSLGSPDRTIQRIFVYHGALITFGGL
                     ILGNVLGLLICGLQQHYGFITLPEDSYFISTAAVKISALQILWVNLGTFLICFTVLLI
                     PSFTLIRKMSPVKALRFD"
     gene            3841..5109
                     /locus_tag="VGM24_03130"
     CDS             3841..5109
                     /locus_tag="VGM24_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HEY4154384.1"
                     /translation="MKYLLIICTVCICGALPAQSPDSVYLPNIQTVQLYLHGNQLAYP
                     VISLNGTDQLDLQFDDLDANVKNYYYTYQLCNADWTPVEVSSFDYIRGFVQSPITDYH
                     YSSIALTRYTHYHISLPAAGSAIIRSGNYLLKVFLDNDTSRLAFTRRLLVVNNAVSIS
                     AQFLQPMNPRISQTHQKIQFTVNSRALTLVNPFQQLKVVLLQNHRWDNALYIDKPSFY
                     SGTNFEYNSDDVSVFPGGKQWRWLDLQSFRFQSDRVAKVDYLPKSTTIYVKPDVDRSG
                     LPYYYYADYNGNYFVQTTESINPYYQTDYATVKFSFIPPDNAAFPDRDIYLFGALTGY
                     QLNEATRMQFDPQKGIYEGTAFLKMGFYNYAYVTVGKNDPTAKTSFEYTEGNHFETEN
                     DYLILVYYRSMGGRADELVGTFSLNTLNPQ"
     gene            5177..5275
                     /gene="ffs"
                     /locus_tag="VGM24_03135"
     ncRNA           5177..5275
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGM24_03135"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5309..>5575
                     /locus_tag="VGM24_03140"
     CDS             5309..>5575
                     /locus_tag="VGM24_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEY4154385.1"
                     /translation="MKFFIDTANLAQIKELNDLGILDGVTTNPSLMAKEGIRGEEKIT
                     AHYKTISDLVKGDVSAEVISTDFDGIVAEGKKLAKINPNIIVKVP"
BASE COUNT         1416 a         1300 c         1449 g         1410 t
ORIGIN      
        1 gccagtttca gggaggggtt agcgagcagc atcttgtcca ggctgtctgt tgtattgagc
       61 ccggtatatt caatattcaa cggtttccac aaaaaagtgc gagttgagtt aacattcagt
      121 tccccttccg tgtgctgccg ggaccgggac cactggtaac cgaaagatcc gttaatggac
      181 cgcagcttga aaaacgccct gcggtccgtg taggaggcat tcacattgat aacggtctgg
      241 gccatccgta atcttccttc gcgcctgatc gggaaaggcg agatgatccg cggaatgctg
      301 atggtatgag acaggctggc cagcgtggtc tgaataaagc tcgaaccgaa ttccaccccg
      361 ccccgcagat tggtattggt ctgaacagat tgtttgtacg cattcctgtt ttgcagtccg
      421 aaattaatcc ccgttccaaa caggttggag gtgctgacga tatcattggt attccggctg
      481 gcttccagat caacggtcag tttttgtttt tttgccggat acatgcgcag gttgacgtcc
      541 agcagggaat cggcgacggg cgaatttaga aactcaatat tattgtattg ccaggccgaa
      601 aactggttga tgcggttgaa cgtgcggtaa taattctcct gcttgtataa tcttccgggc
      661 cttagaaaaa tattatttgc cagaaaggga agcttgaatt tatttgaccg cgttacaaac
      721 tgtaaattcc ggatcacggt ggtatctgtt tttaccgcga tggtatcttc tattacgggc
      781 agatcgggat aaacggtcac tttcccgata taatatttca ggagatgggt cgtgtcccgg
      841 atatcccttt gcttaacggt aacgttgatg gtcgggtttt cgtttttcaa ccggagcttc
      901 tccagcagtt ccgcctgctc cagggggtcc agggagggat caatcagggc ggcaaaaacc
      961 gtgtcgtgct ccacatacag gtcatctttt gtgtagtggt aatatccgtg attgcggaac
     1021 gtatccacca gccggttaat ttctgttacc agtacctgcc tggaataggg ttgtcctttc
     1081 tttatcacag actgactccg ggaatccagg gttaactttt gtaattccgg agaacggagt
     1141 gcatacccaa ccgaatcaaa gaccagttgc tttcccggtt ttaccttgaa atcaatggtc
     1201 acccgctgtt cctccctgct ctgtccccgg tatttttttc tgctgtatac cttgccttta
     1261 tgaacggttt ttattttcac cgtatcctcg atcaccgggg aatagtagcc gttggcattc
     1321 aggagggacg tcatgaacgc gatggagcgg cccaggttaa gggaatcgta caccggcggg
     1381 tgcgatagtt ttttgtagat gatgtgggaa tgccagggcc agtcaaaggc ggtaatggtt
     1441 ttgacctgaa gactgtcatc cagctgattt tccaggcgaa ggatcaggtc ctgccgctga
     1501 tcattcttca ttttcccttc gacctttata tttgtccggt aaacgaaggg cttgccaggc
     1561 tgaaaatctt ttgggatccg gcaggaaaaa agccccagaa agagaatcgc cggaacgatt
     1621 gcaggtctga tcactgatgc tgtaacttgc cggcaatatg acatagaatg taaacgggaa
     1681 acagatgctt gtaaaatcgc aggtcaaata tatccaaagt ttaggccaca aaaaattccg
     1741 ggatgccgaa ggggtctttg ttgcagaggg gccccggatg gtgcaggaac tgttacattg
     1801 tgaatttact gcactcgtta aagcgtatgc gacccgggat tggctattgg aaaatgcatc
     1861 gctgattccg gataaaaaca gcatcacgga agtggatcag caggaactgg aaaggatctc
     1921 ttttttatcc gcggcagttt ccgtgctggc cgtttttcgc aagcctgtct ttccggtgat
     1981 ggataaaccg gaagggatca cgctcctgct tgataatatc caggaccccg gtaacctggg
     2041 aacgattatc cggactgccg attggtttgg tgtgcggcgg atagtatgca gtccggacac
     2101 ggccgatctc ttcagcccga aagtggtgca gtccaccatg ggaagcatta cccgggtgga
     2161 agtgctgtac caggacctga cggaatatct ggacgcgcat cccggtatcc cggcttatgc
     2221 cgcgatgctg gatggagaag atctggaaca gttgcaacgg attccggaag gaatgatcct
     2281 gatcggaaat gaatccaggg gagtgcggcc ggagctggcg gaacgggccg gacggcggat
     2341 ccgcattccg ggcaggggat cagccgaatc cctgaatgtt gcagtggcca ccggtattct
     2401 tttattcatg atccaaaaaa gctagtcgaa ccgcagggct ttcaccggac tcatttttct
     2461 gataagcgta aaactgggta tgagtaaaac ggtaaagcag atcaggaagg tgccgaggtt
     2521 cacccacagg atctgtaagg ccgagatctt tactgccgcg gtggaaataa aataggaatc
     2581 ctccggaagg gtgataaatc cataatgttg ctgcaacccg cagatcagta gtcccagaac
     2641 atttcccagt atcaggccgc cgaaagtgat cagcgccccg tgataaacga agatccgctg
     2701 tatggtccgg tccggagagc cgagggattt taaaaggccg atcatatttg ttctttcaag
     2761 caaaagaata ataagacagg tgaccagatt cagtatggct accaggatca tgatgatgat
     2821 cacgatggcg atggtggtat cctgaagatt gagccagtcg aagatattcc cgtagatttc
     2881 agaaaccgac aggctgttcc atccgggagg aagcttataa taaatacggt tgctgatgga
     2941 gtcggccatt tggtagtcct taataaaaag ttcataaccg ctgatttcat tcgctgtcca
     3001 gttgttcaag cgctggatca ggcggatatc cacgatcgca ttaatgcggt catagtcttc
     3061 aatccctgtt ttaaagatcc cggccacttt cagcggacgc acgcggcggg aacggtcggg
     3121 ctggatgaaa taggcaaaca gtttatctcc gacatgaagg ttgagctggt cggcggtata
     3181 ggaagaaagg tcgatttcac tgctgtacgc ggtatcgggg aagcggatcc agtgcccgga
     3241 tttcaggaag gcctgcaggt gctggaaccg gtagtttctg ccgacacctt tcagccgaag
     3301 cccctcaaag ctctcgggtc ctttgataat ggcatatttg gtggcgaatg gctgcacggt
     3361 taagacttcc ggatcggcgc gaatcgtttg cagcaccgta tcgttggccg taatgggcag
     3421 gtcttcggca atatccaccc gctccggttc ataatgctgt acccggatat gaccggagaa
     3481 gctatacatc ttctgcgcga tcgcatcctg gaatccctgg gtgaaagcaa tcgtcaggat
     3541 catggccatc acgctgatag ccgttgctgc caccgcgagg cggatgataa acctggaaaa
     3601 ggaatgttgc cggttaaaag caacccggcg cgcaataaaa gaagaaagat tcaataaacg
     3661 cgtttttcgg gtcaaaaata gacttccccc cgctgccaga aaagtttatt accgaattgc
     3721 actatcattg catcagctaa ttccaatacg gggtcctgga gaaaaaaggt attaaattat
     3781 aaatgttgag aatttttttc cggcaggaag ggccggccaa ataacgaaaa aaaattacag
     3841 atgaaatatc tgctgatcat ctgcacggtc tgtatttgcg gcgcgcttcc cgcgcagtcg
     3901 ccggattcgg tttacctgcc taatatacag accgttcagc tttacctcca tggcaatcag
     3961 ctggcttacc cggtaatcag cctgaacggg acggaccagc tggaccttca gtttgatgac
     4021 ctggatgcga atgtgaaaaa ctattattat acctaccagt tgtgtaatgc agactggacg
     4081 ccggttgagg tcagcagttt tgattatatc cggggatttg tccagagtcc cataacggat
     4141 tatcattatt catccattgc cctgacccgc tacacccatt atcatatttc gctgccggca
     4201 gcgggttcgg ccattatccg gtccggcaat tatctgttaa aagttttcct cgacaacgat
     4261 acgtccagac tggcattcac ccggaggctg ctggtggtga acaatgcggt ttccatttcg
     4321 gcgcagttcc tccagccgat gaaccccagg atttcacaaa cccatcagaa aattcagttc
     4381 accgtcaaca gccgggccct gaccctggtg aatccatttc agcagctgaa agtggtattg
     4441 ctgcagaacc atcgctggga taatgcgctt tatattgaca aaccctcctt ttattccggg
     4501 accaatttcg aatataacag tgatgatgta tcggtatttc ccggaggaaa acaatggcgc
     4561 tggctcgacc tgcaaagttt ccgcttccag agcgaccggg tcgccaaagt ggattacctt
     4621 ccaaaaagca caacgattta cgtgaagccg gacgtggacc gttcgggatt gccgtattat
     4681 tattatgctg attacaatgg gaattacttt gtgcagacaa cagaaagcat caatccgtat
     4741 tatcaaacgg attatgcaac ggtaaaattc agttttattc caccggataa tgctgctttt
     4801 ccggaccgcg atatatatct tttcggggcc cttacgggat accagctcaa tgaggccacc
     4861 cggatgcagt tcgatcccca aaaaggcatc tatgagggga cggcgttttt aaaaatggga
     4921 ttttacaatt atgcttatgt gacggtcggg aaaaacgatc ccactgccaa aacctccttt
     4981 gaatatacgg aaggaaacca ttttgaaacg gaaaatgact acctcattct cgtttattac
     5041 cgcagcatgg ggggaagggc cgatgaactg gtggggacct tttcgctgaa cacgctgaat
     5101 ccccaatgag aaggccggcg gtcagccaga ccttttccgg atttccaccc ttagttcaat
     5161 tgtcttatct ttgcagcggc aagtcttata cggccagctc ctgctaaatc ccccagggcc
     5221 ggaaggcagc aagggtaggt ggtcgtagcg gtgcgatgta agtaacttgc catttttttt
     5281 attcttatca atcgaataaa tatatagtat gaaatttttt atcgataccg ccaacctggc
     5341 gcagatcaaa gaactcaatg atctgggcat actggatggg gtaaccacga atccttccct
     5401 gatggccaag gaaggaatcc ggggagaaga aaagatcacg gcgcattata agaccatttc
     5461 cgacctggta aagggagacg tgagcgcaga ggtgatttca accgattttg acggcattgt
     5521 cgcggaaggg aagaaactgg ccaaaatcaa tccaaatatc atcgttaagg tcccg
//
LOCUS       DASYCU010000158         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1923848, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000158 DASYCU010000000
VERSION     DASYCU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923848"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGM24_03145"
     CDS             <1..336
                     /locus_tag="VGM24_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154386.1"
                     /translation="CITLKDRKGGIVSVLVDQKMNVKDLKVGEPDMAPVEKIESIFTV
                     SPTYKNGNRISYTSTKPGEYEIYFSIGEPDGTPCLELPYDNDDGHKRYKVGTIKVAAR
                     EAGTASPEQ"
     gene            380..979
                     /locus_tag="VGM24_03150"
     CDS             380..979
                     /locus_tag="VGM24_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HEY4154387.1"
                     /translation="MYPVKNSISLFAFMIVAISASAQQTGRIKDVRDGKTYKSVKIGR
                     QTWMAQDLNFYTKTGSWCFENDSSSCDKSGRRLYTPIAAAEACPAGWHLPNADEWMEL
                     VNFLGGPAVAGGKLKAKTGWNASDSAASGSSGFSALPGGYRNYGGVFGGAGTEGYWWS
                     ASTLGRGEWYFSMRYDDNAIRKYGSNHKESGYSIRCVKN"
     gene            1032..1970
                     /locus_tag="VGM24_03155"
     CDS             1032..1970
                     /locus_tag="VGM24_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154388.1"
                     /translation="MEKNKISKESFSIHPGDQEKHEIRNERRRFLKNISALTAAFTVS
                     QAFGSVRLPSGLMKGTGSSSPAAIHPDKDGVFIISEPEHTPEQIAKAIAEIPKIEYVP
                     SSERWINLPRTAAALAQDGGELKVVMLGDSIINDTYRSAWYTLLQAQYPKCKINAVAV
                     VRGSTGCWWYEEKGRIKHYVLPLKPDLLIIGGISQKNDIKAIKKVIHQVRSAQPCDVL
                     LVTNVFGQEDPNNDKQWSFDIPSVPGNYRKGLLDLSVEAKTGFFDMNAYWGRYMRNSG
                     HPIDWFHRDEVHANLRGEQVIGHLMAGYLSPATSAG"
     gene            2025..>2227
                     /locus_tag="VGM24_03160"
     CDS             2025..>2227
                     /locus_tag="VGM24_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154389.1"
                     /translation="MRISVRYFSGFLLLFLMADLCRGQAVLTEQQDRWVITPDSSIEW
                     KIDGRLPHHDHIEMSGEKVSLWV"
BASE COUNT          619 a          508 c          610 g          490 t
ORIGIN      
        1 tgtatcacgc tgaaagaccg gaaaggcgga attgtatccg tgctcgtgga tcagaagatg
       61 aatgttaaag atttaaaagt tggagagccg gatatggcgc ctgttgaaaa aatagaatcc
      121 atctttaccg tatccccaac ctataagaat ggaaaccgga tctcttatac aagtacaaaa
      181 cccggtgagt acgaaattta tttttccata ggcgaaccgg atggaactcc gtgcctggaa
      241 ttaccttatg acaacgatga cggacataaa aggtataaag tgggcaccat aaaagttgcc
      301 gcgagggaag ccggcactgc gtcccctgaa cagtaagggt gatatgaaca acacactaaa
      361 atgaaaattt aaaaaaacga tgtatccggt aaaaaattca ataagcctgt ttgcttttat
      421 gattgtcgcg atcagtgctt cagcacagca aacaggcagg attaaagatg tgcgggatgg
      481 caaaacctat aaatcggtaa agatcggcag gcagacctgg atggcgcagg acctgaattt
      541 ctataccaaa acgggcagct ggtgctttga gaacgacagc agcagttgcg ataaatcggg
      601 ccggcggttg tacaccccga tagcggcggc ggaagcctgt ccggccggct ggcacctgcc
      661 caacgccgac gaatggatgg agctggtcaa ttttctcggt ggcccggctg tcgcgggcgg
      721 aaagctcaag gctaaaacgg gttggaatgc ctccgacagt gctgccagcg gcagcagcgg
      781 tttctcagca cttccgggag gataccggaa ttatggcggc gtattcggcg gtgcgggcac
      841 ggaagggtac tggtggtccg ccagcacctt gggcagaggg gaatggtatt tctccatgcg
      901 ttacgacgat aatgcgatcc gcaagtacgg cagtaatcat aaagagtcgg ggtatagtat
      961 ccgctgtgtg aagaattgaa ttaccgggga gaccggttaa cagaattata aataaattta
     1021 aaacaggaaa aatggaaaaa aataaaatca gcaaagagag cttttctatc catccgggtg
     1081 atcaggaaaa gcatgaaatc aggaatgaaa gacgccgctt tctgaaaaat atttcagccc
     1141 tgacagcggc ctttaccgta tcgcaggctt ttggatcggt ccgcctgcca tcgggactga
     1201 tgaagggaac cggttcatct tcccctgccg ccattcaccc cgacaaagac ggcgtattca
     1261 tcatcagtga gccggagcat acccccgaac agatcgcaaa ggctatcgct gaaattccca
     1321 aaatcgaata cgttccttcc tccgaacggt ggatcaacct tccgcggaca gcagccgcgc
     1381 tggcacagga cggaggggag ctgaaagtcg taatgctggg ggacagtatc atcaacgata
     1441 cctataggtc tgcctggtac acccttcttc aggcccaata cccgaaatgc aagatcaacg
     1501 cggtggccgt ggtaagagga agcaccggtt gctggtggta tgaggaaaag ggaaggataa
     1561 agcattatgt gcttccgctt aagcctgact tactgatcat cggcgggatt agccagaaga
     1621 atgatattaa agcgatcaaa aaggtgatcc accaggtccg ttcggcccag ccatgcgatg
     1681 tattgctggt aaccaatgtt tttggccagg aagatcccaa taacgataaa caatggagct
     1741 ttgacattcc gtccgtgccg gggaattacc ggaaaggcct tctggatctg tcggtggaag
     1801 caaagaccgg cttctttgat atgaatgcct actggggccg ctacatgcgt aattcagggc
     1861 atccgatcga ctggtttcat cgcgatgaag tgcatgcaaa cctgcgtggt gaacaggtga
     1921 tcgggcatct gatggccggt tatctttcgc ccgccacctc cgcgggttag gaagatccgg
     1981 gtcgaagaaa tccgccgggt attaaaagcg aatcagaaag cattatgaga atcagtgtaa
     2041 gatatttttc cggttttctc cttctttttc ttatggcgga cctttgccgt gggcaggccg
     2101 tattgacgga gcaacaggat cgctgggtga ttactcccga tagcagcatt gaatggaaga
     2161 tagacggccg gcttccccat cacgatcata ttgaaatgag cggtgaaaaa gtttccctgt
     2221 gggtcca
//
LOCUS       DASYCU010000159         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3807120, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000159 DASYCU010000000
VERSION     DASYCU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3807120"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1356)
                     /locus_tag="VGM24_03165"
     CDS             complement(61..1356)
                     /locus_tag="VGM24_03165"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 domain-containing protein"
                     /protein_id="HEY4154390.1"
                     /translation="MIRLWKFVLPVVYVLMMAFPVAAQGPAVGPSFIMTLTNPLAIVR
                     PDEPMVWRRADLEKKMGPIHAGKFIRITEENGAPVIVQFDDLDRDGQWDEIFWLHSFQ
                     PMESLHVRVMIADAPAAIKAVVRAHVRMRAKNEGNNFGPDLDSAVMPVHNQPTDFSVH
                     RLPPWLTEGPGWENDKVAYRLYFDTRNDKDIYGKRIPAMVMDEVGTDPDKSYHELADW
                     GMDILKVGTSLGAGALAVLVPREGKKDSLVRLGGLGVKRETFRKISDGPLRAVFRMDY
                     EWELEGRPVIVTEEMSISGGQYYYTSKVTVNGAPNGSRLVTGIANFYSNSPGNFGEDN
                     AEVLYSHGRQSENKDMLGMAILTGKSDFSSFRSLPASGAEVTSTYTISQYIRKDNPLY
                     FRFYTGWEKTDTGFASPEYFINMLKIEADKFSHPVQVQW"
     gene            1617..2249
                     /locus_tag="VGM24_03170"
     CDS             1617..2249
                     /locus_tag="VGM24_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4154391.1"
                     /translation="MSKAKKTVVEEVSTEEKIKAAAKKLFTEKGFAETRTRDIAEEAG
                     INLALLNYYFRSKQKLYDIIMLENFRQFIQGVSLNLFDENTSIEEKIEKVVIAYIDFL
                     TLNPNLPLFIINELKGNPSKIAAQINEEISPLRSYFIKQLQEAGKEGKIASMDPFHFI
                     ANLVGLTVFPFISKPLLQRITKTNEEQFQAFMQERKKLVPIWIRGILTVK"
BASE COUNT          672 a          678 c          461 g          557 t
ORIGIN      
        1 atccattaac cggccaggta attctcattt cagcacaaaa aagatcaccg tcgcctaaaa
       61 tcaccactgc acctgtaccg gatggctgaa cttgtccgct tcgatcttta acatgttgat
      121 aaaatattcc ggcgaggcaa agccagtatc cgtcttctcc caacccgtat aaaaccggaa
      181 atataaggga ttgtccttac gaatatattg agagatcgta taagtgctcg tcacctccgc
      241 cccgctggcc ggcaggcttc tgaaagagct aaaatcactc ttcccggtca ggatggccat
      301 gcccagcata tccttgtttt cggactgccg gccatgtgaa tacaacactt ccgcattatc
      361 ctccccgaaa ttacccggtg aattggaata aaaattagca atccccgtca ccagcctcga
      421 tccattcggc gcaccattca ccgttacctt gctggtataa taatattgac caccgctgat
      481 gctcatctct tccgtcacta taacgggtcg gccttccaac tcccactcat agtccatccg
      541 gaaaacggct cgcagcggac catcggagat cttccggaaa gtctcccgct tcacccccag
      601 gcctcccagc ctcaccaggc tgtccttctt tccttcccgc ggcacgagaa cagccaacgc
      661 ccccgcccca agcgaagtcc ccaccttcag gatatccata ccccaatccg ccagctcatg
      721 atagcttttg tcaggatcgg tacccacctc atccattacc atcgccggta tccgcttgcc
      781 ataaatatct ttgtcattac gggtgtcgaa atacaacctg tacgccacct tatcattctc
      841 ccagccaggt ccttccgtca gccagggagg caaacgatga acggaaaaat ccgtcggctg
      901 attatgcacc ggcatcaccg cactatccag atccggcccg aaattattgc cctcattctt
      961 tgctcgcata cgtacatgcg ccctcaccac cgccttgatc gccgcaggag catccgcgat
     1021 catcacccga acatgtaacg actccatcgg ctgaaaagaa tgcagccaaa aaatttcatc
     1081 ccactgtcca tcccgatcca ggtcatcgaa ctgtacgatg acaggcgctc cattctcctc
     1141 ggtgatccgt atgaactttc ccgcatgtat tggccccatc ttcttttcca gatccgccct
     1201 cctccacacc atcggttcat caggccgtac aatggcaagc ggattggtaa gcgtcatgat
     1261 aaaagaaggc cccactgcag gcccctgcgc cgcaacagga aaggccatca tcagaacata
     1321 caccaccggc agaacgaact tccatagtct tatcatattc aaaatttcaa ggaccttaaa
     1381 tgtaaggctt attgacgaaa gagcaccaac gttggagtgc cccggtctat gaccagtagt
     1441 attagcccac ttccgcgaat accacaaatc ggtcctggcg aatgaaattg gcttcttctt
     1501 aatattcaat tagttggatg ccttttcctg attcatttca gcgttttaat caaatgatta
     1561 aaaattttgt ttaataattc aaaaatacga ttaactttgt gctacaatag caggatatga
     1621 gcaaggcaaa aaagaccgta gtggaagaag tatcgaccga ggaaaaaata aaggcggctg
     1681 ccaaaaaatt gtttaccgag aaaggattcg cagagacccg gacacgtgac atcgcagaag
     1741 aagccggcat caacctggcc ctgctgaact attacttccg cagtaaacaa aagctctatg
     1801 acatcatcat gctggaaaat ttccggcagt tcatacaggg agtctcactc aaccttttcg
     1861 acgaaaacac cagcatcgaa gaaaagatcg aaaaagtcgt catcgcgtat atcgacttcc
     1921 tgaccctgaa ccccaacctg ccccttttta ttatcaacga attgaaaggc aatccatcga
     1981 aaatagcagc ccagatcaac gaagaaataa gcccgctgcg gagctatttt atcaagcaat
     2041 tgcaggaagc cggaaaagaa ggaaagattg cctccatgga tcccttccat ttcattgcca
     2101 accttgtagg actgaccgta tttcccttta tcagcaaacc gctgctccaa aggattacaa
     2161 aaaccaatga ggaacaattt caggcattca tgcaggaacg gaaaaagctc gtgccgatat
     2221 ggatcagagg gatcctgaca gtcaaataaa aaattgttca ctttttttaa aatattctgc
     2281 ttgccagttc gtctaacgaa ataagtacga tcccggaatc agtatggcca tgaccaggtc
     2341 gcatcccccg gacgtttttt ttgaacaa
//
LOCUS       DASYCU010000160         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4821739, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000160 DASYCU010000000
VERSION     DASYCU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4821739"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /gene="pfkA"
                     /locus_tag="VGM24_03175"
     CDS             complement(<1..981)
                     /gene="pfkA"
                     /locus_tag="VGM24_03175"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY4154392.1"
                     /translation="MQQKVSKIGVLTSGGDAPGMNAAVRAVVRTGIYYGLEVYGIMRG
                     YSGMIENDIVPLHSRSVANIIQRGGTILKTARCKEFFEYEGRSRAFSNLKKLGINGLI
                     IIGGDGSFRGAHKFSTEFQIPCIGLPGTIDKDIAGTDFTIGFDTAVNTAVEAIDKIRD
                     TADAHDRLFVIEVMGRDAGYIALHSGIATGAENILIPERKTDIEELVGSLQEKEKRKK
                     LVNLVVVAEGDNFGGAEEVAKVVKQRLPNIDTKVCILGHIQRGGSPSCIDRVIASRMG
                     YSAVECLLEGKFNVMVGIVNNKMHYTALDKAVKAKQKISEDWLKIVKILAS"
     gene            1019..2092
                     /locus_tag="VGM24_03180"
     CDS             1019..2092
                     /locus_tag="VGM24_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4154393.1"
                     /translation="MIRHEYLKFFSLSKISPKNKHFEWNQNAGGGLMERKTSNNGSPA
                     GLFPGYKNSLYIFSPVMKILITGSNGLLGQHLISQLAALQHEVIATGRGVPRFAPDQA
                     VRYEDLDITNFRRMAAFIQSESPEVLLHSAAMTQVDECELNREKCRRVNVQSTENLLE
                     AAAPCGTHFVFLSTDFVFDGIKGNYREEDAVGPVNWYGETKVLSEERVKNYPANWSIA
                     RTCLLYGVSRGSSRSNIISWIRSSLEKGERIRVVNDQVRTPTDVNDFATGLRLIIEQK
                     ASGLFHISGRERMTPYQLALETAGHFSLDKTLIEEVNADSFSQAGQRPLKTGFIIEKA
                     EKVLGFSPVPLKTGLANMAFRKG"
     gene            complement(2108..3019)
                     /locus_tag="VGM24_03185"
     CDS             complement(2108..3019)
                     /locus_tag="VGM24_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEY4154394.1"
                     /translation="MQNTSRLLMIRPVHFGYNAQTAVNNSFQVKGDQTGVSENALREF
                     DELVSKLRSFAIDVTVVEDSPEPDTPDALFPNNWISFHDQQILCLYPMYAPNRRQERK
                     PAVLEALSKKFPVRKQIDLTHYENNNQFLEGTGSMVLDRDNRIAYAGLSPRTDLQVLN
                     DFCTQLNYRPCVFHAADEQGQLIYHTNVMMCVADRFVLICLDSIRDAEEKKYVSGTIA
                     STGKEIISISLDQMNHFAGNMLQVENTKREKFLVMSTAAYHALTPEQIEKLSSGNHLI
                     HSSLQTIESNGGGSARCMIAEMHGVPR"
     gene            complement(3028..>3606)
                     /locus_tag="VGM24_03190"
     CDS             complement(3028..>3606)
                     /locus_tag="VGM24_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY4154395.1"
                     /translation="KKRDYMHIDTVFTQVKRDAWVMLGVFSKKSMKHENADPVQRALE
                     GNKKEEKMRILQFRKKDMENPTYYDNLEDLLTDISKQDLQCTGKVKFIYSGNNEFPFD
                     AREQWTDSCNLLALKEGVVLGYDRNDKTVEAFRDNDFDIIEAAELIRQMEAGSVKADD
                     LKDTLILMPSAELSRARGGFHCMSMPLLRDDL"
BASE COUNT          837 a          937 c          874 g          958 t
ORIGIN      
        1 agatgctaat attttgacga tcttcagcca gtcctcactg atcttttgtt ttgctttcac
       61 cgccttgtcc agggcggtgt aatgcatttt attatttaca ataccgacca tgacattgaa
      121 tttgccttcc agcaggcatt cgacggcgga ataacccatg cggctggcga tcacccggtc
      181 aatgcagctg ggtgagccgc cccgttgtat atgcccgaga atgcacactt tggtatcgat
      241 attcgggagt cgctgcttta ctactttggc aacctcctcc gcacccccaa aattgtcccc
      301 ttcggcaacc acgacgaggt tcaccagttt tttccttttt tctttttcct ggagggagcc
      361 caccagttcc tcaatatccg tttttctttc cgggatgagg atattttccg caccggtagc
      421 gatgccgctg tgaagggcta tgtatcctgc gtccctgccc attacttcta tcacgaacag
      481 gcggtcatgc gcatcggccg tatcccggat cttgtcgatc gcttccacgg ctgtatttac
      541 ggccgtatcg aatccgatgg taaaatccgt acccgcaata tctttatcga tggtgccggg
      601 aagcccgata cagggaatct ggaactccgt actgaattta tgtgcgcccc ggaaggatcc
      661 gtctcctccg atgatgatga gcccgttaat gcccagtttt ttaagattgg aaaaagcccg
      721 ggagcggcct tcatattcga agaactcctt gcaacgggcc gttttcagaa tggttccccc
      781 gcgctgtatg atattggcca cgctcctgga atgaagggga acgatatcgt tttcgatcat
      841 cccgctatac ccccgcatga ttccgtaaac ttccagtcca taataaatcc cggtacgaac
      901 aacggcgcga acagcggcat tcattcccgg cgcatcgcct ccggaggtga gtacccctat
      961 ttttgatact ttttgttgca ttaactagta aagggttgta ttcgttgaat cagctttaat
     1021 gatcaggcac gaatatttga aattctttag tctttccaaa ataagtccca aaaataagca
     1081 ttttgaatgg aatcaaaacg caggcggcgg cctgatggaa agaaagacct caaacaacgg
     1141 atctcccgca ggtttatttc ccggatataa aaattcacta tatattttca gtccggttat
     1201 gaaaatattg atcacgggtt cgaatgggtt gctgggccag catctgatat cacagctggc
     1261 cgcacttcag catgaggtga ttgccacggg ccgcggtgtt ccgaggtttg ctccggatca
     1321 ggctgtccgg tacgaggatc tggacataac gaatttccgt cggatggctg cattcatcca
     1381 atcagaatca cccgaggtgc tcctccactc ggcggctatg acccaggtgg acgagtgtga
     1441 acttaaccgc gaaaaatgcc ggcgggtaaa cgtgcagtcc acagaaaatc tgttggaagc
     1501 cgccgcccct tgcggaacgc actttgtatt tctttccacc gattttgttt ttgacggaat
     1561 aaaaggaaat taccgggaag aagatgcggt tggtccggtc aactggtacg gggaaaccaa
     1621 agtgctctca gaggaacggg tcaaaaatta tccggcaaac tggtcaattg cccggacctg
     1681 cctcctctat ggtgtttccc gcggatcttc ccggagcaat atcatttcct ggataagatc
     1741 gtccctggaa aaaggcgagc gcatccgggt ggtgaatgat caggtgcgta caccgaccga
     1801 cgtaaatgat tttgcaaccg gcctccggtt gatcattgaa caaaaagcat ccggtctttt
     1861 tcatatttcc ggcagggagc gcatgacccc ttaccagctg gcgctggaaa cggccgggca
     1921 tttttctctt gacaaaaccc tgatcgaaga ggtgaatgca gacagtttct cccaggccgg
     1981 ccaaaggccc ctgaaaaccg ggttcattat tgagaaagca gaaaaggtct tgggcttttc
     2041 cccggttccc ctgaaaaccg ggctggcaaa tatggcattc cgcaaggggt gaggccccgt
     2101 ttccttctca tcgcggcaca ccgtgcattt ctgcaatcat acagcgcgcg ctaccgccgc
     2161 cgttgctttc aatcgtttga agagaggagt ggataaggtg gttgccggaa gataattttt
     2221 caatctgttc cggggtcagc gcgtggtaag ccgctgtgga cataaccagg aacttttctc
     2281 ttttcgtatt ttccacctgc agcatatttc cggcaaaatg attcatctga tccaggctga
     2341 tggaaatgat ttccttgccc gtggatgcaa tggtaccgga aacatatttt ttttcttccg
     2401 catcccggat ggaatccagg cagatcagca caaagcgatc cgccacgcac atcattacgt
     2461 tggtatgata aataagctgc ccctgctcat cagcggcatg aaacacacag ggccggtaat
     2521 tcaactgcgt gcaaaaatcg ttcagcacct gcagatctgt gcggggcgac aggcccgcat
     2581 aagcgatccg gttatcccgg tcgagcacca tgctgccggt cccttccaga aactgattgt
     2641 tgttttcgta gtgcgttaaa tcaatttgct ttcttaccgg aaattttttc gaaagggctt
     2701 ccagtacggc gggctttctt tcctgcctcc ggttcggggc atacatgggg taaaggcaaa
     2761 ggatctgctg gtcatggaac gagatccagt tattcggaaa gagagcatcc ggcgtatccg
     2821 gctccgggct gtcttccacc acggtcacgt caatagcaaa agaccggagt ttactgacca
     2881 gctcatcaaa ctcccgaagg gcattttcag aaacacccgt ctgatctcct tttacctgga
     2941 acgaattatt cacggcggtt tgggcattgt aaccaaaatg caccggacgg atcatcagca
     3001 aacgggatgt attttgcata tattcattta gaggtcatcg cgaagcaggg gcatgctcat
     3061 gcagtgaaaa ccgccgcggg ccctcgagag ttctgcagaa ggcatgagga tcagggtgtc
     3121 cttgagatca tcggctttga ccgatcccgc ttccatttgc cggatcagtt ctgcggcctc
     3181 aatgatatca aaatcgttgt cccggaatgc ttccaccgtc ttgtcgttcc ggtcataacc
     3241 cagcacaacc ccttctttca gggccagcag attgcaggag tcggtccatt gctcgcgcgc
     3301 atcaaaagga aattcattgt tcccggaata gatgaatttt accttgccgg tacactgcaa
     3361 atcctgtttg ctgatatcgg tcagcaaatc ttccaggttg tcgtagtagg tgggattctc
     3421 catatctttt tttcggaact ggaggatcct catcttttcc tccttcttat tcccttccag
     3481 cgcccgttgt acgggatccg cattttcgtg tttcatggat tttttggaga atactcccag
     3541 cattacccag gcatcccgtt tcacctgggt aaataccgta tcgatatgca tgtaatctct
     3601 tttttt
//
LOCUS       DASYCU010000161         4994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_910889, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000161 DASYCU010000000
VERSION     DASYCU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910889"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..393
                     /locus_tag="VGM24_03195"
     CDS             103..393
                     /locus_tag="VGM24_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154396.1"
                     /translation="MDKKIKKTVAESGEDKKFPLPKYPADEDIYNKEKEESFEELDLK
                     SQKLKNEMAEGLDVPGADLDDENEVIGEEDEENNYYSLGGDNHSDLEEDNGE"
     gene            408..965
                     /locus_tag="VGM24_03200"
     CDS             408..965
                     /locus_tag="VGM24_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEY4154397.1"
                     /translation="MPTKKKIPTAWLMIIVGFVFIAIGVFVFLYPLHAYYRLAKFTGL
                     LLLLNALLLLYIAITRTRNNQERNWILAEAVIDFLFVLLFLFNAFLSFLALPFFIATW
                     MGIIGGLKVAACLSLKKIIRGWGFIFMEGIISLLFSFWVFHAPSPKAASITIPIGLFS
                     LLMGAFNMIEAVRFKKRESTLDMML"
     gene            1023..2576
                     /locus_tag="VGM24_03205"
     CDS             1023..2576
                     /locus_tag="VGM24_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY4154398.1"
                     /translation="MKMVFAFLFFGFIPAAFYGQPSGAAAIVRTEAGRISGSRDASGK
                     ISIYKGIPFAAPPVGDLRWKAPQPVKHWNQVKECNAYGPSPMQNKPVPFSMWTEEFLI
                     AKEPISEDCLYLNVWTPTASATEKRPVLVWIYGGGFSSGGSSAPIYDGEAMAKKGLLF
                     VSINYRVGIFGFLADPELTKESGHEASGNYGLMDQIAALKWVKQNIAAFGGDPEKVTI
                     AGQSAGSMSVNSLVASPLARGLFVRAIGESGASFTKNPKSSGLANAEEQGTKIAQSLG
                     NGTIASLRKIPAEELLQKAKGSFSPVIDGYVLPASIAQIFNSGKENPVDLLTGWNQDE
                     GFLSGKIKDAGAYRKEAEEKYGADADVFLSLYPGKTDSEAAASQLKVSRDMTFGVQNY
                     TWANRQSKQNGSRVYVYRFTRKLPATGEYVKYGAFHTGEVPYAYDNLKFVNRPWEPVD
                     FQLAKMMSSYWANFVITGNPNGSGLPEWPAYNSNSKMIMFLEEKPGAAEMPDYKGLDF
                     IYRKMTGGN"
     gene            complement(2573..3202)
                     /locus_tag="VGM24_03210"
     CDS             complement(2573..3202)
                     /locus_tag="VGM24_03210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY4154399.1"
                     /translation="MSLSNAYKIETERLIIRCYEPGDAAKLHEAITLSMDHLSPWMPW
                     INQEAGAVAGKLDRIRLFRGEFDLGKDYVFGIFNKSEDQLIGSTGLHTRVGEGAREIG
                     YWISARHLNQGFALEAVSALIKTGFEIEKLFRIEIHCNVENIRSQAIPRKLGFRLEAI
                     LKSNPLDADAFPRETMIWSLLKSDYDNSPVKKTRIRAFDIAGQEIDNPG"
     gene            complement(3231..3554)
                     /gene="trxA"
                     /locus_tag="VGM24_03215"
     CDS             complement(3231..3554)
                     /gene="trxA"
                     /locus_tag="VGM24_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY4154400.1"
                     /translation="MSAGKGSFHDLIHGDQPVLVDFSAEWCGPCKMMPPILKELKDAL
                     KDSIRIIKLDIDRNPELARVYQIQSVPTLIIFQNGEIKWRQSGVIPARQLQQILEQTM
                     QKTPH"
     gene            complement(3623..4081)
                     /locus_tag="VGM24_03220"
     CDS             complement(3623..4081)
                     /locus_tag="VGM24_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4154401.1"
                     /translation="MTAERAHEVKNKTSAREYILLAEDDVDDVEFLTDSLQNLDQSLE
                     VLVISSGDKAIPFFDQVPDDHLPGLIIMDYNLPAMSGYQILTILAGNERYKNIPKIIW
                     STSNSSFFEKECLTNGATAYFVKPADLSGYEELAEKILDFLKSREWKTED"
     gene            4166..>4994
                     /locus_tag="VGM24_03225"
     CDS             4166..>4994
                     /locus_tag="VGM24_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY4154402.1"
                     /translation="MNNKKKIDREKAEGVNAGSLVKTPTAARTKPRSKNGETGANLSL
                     NGHRFSPEGHLNSRELLSVLSEMRNGNFSARMPVDLLGLDGKICDTLNEIISLNEALM
                     LELTKAGNIIGRQGNLTHRLVLPQIARGRWSDGIDSINELISNLVHPTIEIAHVISSV
                     AKGNLSEEMHLQIGNHKLQGEFLRIAREVNDMVKQLNLFSMEVTRVALEVGSEGKLGG
                     QAKVKGVAGVWKDLTDSVNQMGSNLTAQVRNIAEVTTAVARGDLSRKITVDVKGEIQE
                     "
BASE COUNT         1344 a         1196 c         1247 g         1207 t
ORIGIN      
        1 cggggaacgg tttaaaaccc gataattaaa ctaaattgtg gcggaaggcc atttaccgcg
       61 gctggaatgg gtattgaagt gcaaaaaatt aaaataaatt ttatggacaa gaaaataaag
      121 aagacggtag cagagagcgg ggaagataaa aaattcccgc ttcccaaata cccggcggat
      181 gaggacatct ataataagga aaaagaggaa tcctttgaag agctggattt gaaaagtcag
      241 aagctgaaaa acgaaatggc cgaagggctc gacgtgcccg gcgcggacct ggacgatgaa
      301 aacgaagtta tcggggagga agatgaggaa aataactatt acagtcttgg cggagataac
      361 cacagcgacc tggaagaaga taacggagaa taatattaag attccccatg ccgactaaaa
      421 aaaagattcc gaccgcctgg ctgatgatca ttgttggatt tgtatttatc gcaattggtg
      481 tttttgtatt tctttatccc ctgcatgcct attaccggct ggcaaaattt acgggcctgc
      541 tgttgctcct caatgcgctt ttgctgttat atattgccat caccaggacg cggaataacc
      601 aggaaagaaa ctggatcctg gcagaagcag tcattgattt cctgttcgtg ctgctttttt
      661 tatttaatgc ttttctatcc ttcctggcgc ttcccttttt tatagccacc tggatgggga
      721 tcatcggcgg actgaaggtt gccgcctgtc tttccctgaa aaaaataatc aggggctggg
      781 gatttatttt tatggaaggg atcatttccc tcctgttttc cttctgggtc tttcatgcgc
      841 cttctcccaa ggcggccagc atcaccattc cgatcggttt gttctccctg ctgatgggcg
      901 cattcaatat gatcgaggcc gtccggttta agaaaagaga aagtacgctc gatatgatgc
      961 tctgaaacgg gcaatttttt taaataatga tttaatttca ttttgtatca atcacggatt
     1021 atatgaaaat ggttttcgcc ttcctgtttt ttggctttat cccggcggca ttctacggtc
     1081 agccatcggg tgccgcggcg atcgtaagaa cagaagcagg gcgcatttcc gggagccggg
     1141 atgcatccgg taaaatatcc atttataaag ggatcccctt cgctgcaccg cccgttggcg
     1201 acctgcggtg gaaagcgccg cagccggtta aacactggaa ccaggtaaaa gaatgtaatg
     1261 cttacgggcc cagcccgatg cagaataaac cggttccttt cagtatgtgg acggaagaat
     1321 ttctcattgc caaagagccc atcagcgaag attgccttta tttgaatgta tggacgccca
     1381 ctgcctcagc caccgaaaaa agacctgtcc tggtttggat ctatggcggc ggcttttcca
     1441 gcggaggcag ttccgctccc atttacgacg gcgaagccat ggcaaaaaag ggcctcctgt
     1501 ttgtaagcat caattaccgg gtgggcatct tcggattcct tgcagacccg gaactcacga
     1561 aagaatcggg tcatgaagca tcggggaatt acggattgat ggaccagatc gctgccctga
     1621 aatgggtgaa gcaaaatata gccgcattcg ggggagatcc ggagaaagta accattgccg
     1681 gacaatccgc cggttccatg agcgtgaaca gcctggttgc ctccccgctc gccaggggat
     1741 tgtttgtccg ggcgatcgga gaaagcgggg catccttcac gaagaatccg aaatcgtcgg
     1801 gtttagcaaa tgcagaagaa cagggcacca aaatagcgca gtccctgggc aacggaacca
     1861 ttgcttcatt aaggaagatt ccggcggaag aattgttaca aaaagcaaaa ggatctttct
     1921 ccccggttat cgacggttac gtattgccgg cttccattgc acaaatattc aattcaggaa
     1981 aggagaaccc ggttgactta ctgaccggct ggaaccagga tgaaggtttt ttatcgggca
     2041 agataaaaga tgccggggct taccgaaaag aggcagagga aaaatatgga gcagatgccg
     2101 atgtttttct gtccttatat cccggcaaga cggattcaga agcggcggct tcgcaattaa
     2161 aggtttccag ggatatgaca ttcggcgttc aaaactatac ctgggcgaac aggcagagta
     2221 aacaaaatgg ctcccgcgtt tatgtatatc gatttacccg caaacttccg gccacgggag
     2281 aatatgtgaa gtacggcgcc tttcataccg gcgaagttcc ctatgcttat gataatttaa
     2341 aatttgtaaa ccgcccctgg gaacccgtgg attttcaact ggcaaagatg atgtcgtcct
     2401 attgggcaaa ttttgtcatt accggaaacc cgaacggatc cggacttccg gaatggccgg
     2461 cctataacag caacagcaaa atgattatgt tcctggaaga aaagcccggg gccgcggaaa
     2521 tgccggacta taagggactg gattttatct atcgaaaaat gaccggggga aattaaccgg
     2581 ggttatcaat ttcctgaccg gcaatatcaa aagcccggat ccgggtcttt ttaacggggc
     2641 tgttatcata atccgattta agcagcgacc agatcatcgt ttcccggggg aaggcatcgg
     2701 catccagcgg attgcttttt agaatggctt ccagcctgaa ccccagcttt ctcggaatag
     2761 cctgactgcg tatattctcc acattacaat gaatttcgat ccggaagagc ttttcaattt
     2821 caaaaccggt ctttatcagc gcactcaccg cctccagggc aaaaccctga ttcaggtgcc
     2881 gggcggagat ccagtaaccg atctcccttg ccccctcccc gacgcgggta tgcaaacccg
     2941 tagagccgat cagctgatct tcggatttat taaaaattcc gaaaacataa tcctttccca
     3001 gatcgaattc cccgcggaaa agccttatcc ggtccagttt tcccgccacc gccccggctt
     3061 cctggttgat ccagggcatc cagggcgaca aatgatccat gctcagcgta atggcttcat
     3121 gaagcttcgc cgcatcaccg ggttcataac aacgaatgat gagcctttcg gtctcgatct
     3181 tataggcatt tgataaactc atggcgttgt accggcattt taccggcaaa ctagtgaggt
     3241 gttttctgca tggtttgctc caggatctgt tgcaattgtc tggcgggaat caccccggac
     3301 tgccgccatt taatttctcc attctgaaaa ataatcaggg tcggtacact ttgtatctgg
     3361 taaacccggg ccagttccgg attccggtcg atatccagtt taatgatccg gatggaatct
     3421 tttaaggcgt ctttcaattc tttgagaatc ggaggcatca tcttgcacgg cccgcaccac
     3481 tctgcagaaa aatccaccag caccggctga tcgccatgga tcaggtcatg aaaagatcct
     3541 tttcccgcgg acataagaat tcattttccg aaagttaacg attttcgtcc gcatcggggt
     3601 atctccggcg gttaacgaga actcaatctt ccgttttcca ctccctgctc tttaaaaaat
     3661 ccaggatctt ttctgccagc tcttcatacc ccgaaaggtc ggccggcttt acaaaataag
     3721 cggtggcccc gttggtcagg cattcctttt caaaaaaaga agaattggaa gtactccata
     3781 tgattttggg aatgttttta tatctttcat tcccggcaag gatagtcagg atctggtacc
     3841 cgctcatggc aggaaggtta taatccatga taatcaatcc cggaagatga tcgtccggca
     3901 cctggtcaaa aaacggaatg gccttatccc cgctgctgat taccagtact tcgaggctct
     3961 ggtccagatt ttgcagcgag tcggtaagaa attccacatc atcaacatca tcttctgcca
     4021 gcaatatgta ttcacgggct gaggtcttat tcttaacttc atgcgcgcgt tccgccgtca
     4081 tgtaaatggt ttagaaagca atatacgaag caattaaaat aatcaaataa tcaggctgcg
     4141 attataatta aacaccggat ggtttatgaa taataaaaag aaaattgacc gggaaaaagc
     4201 ggaaggggtc aacgccggct ccctggtaaa aacgccaacg gccgccagaa caaaacccag
     4261 gtctaaaaac ggagaaacgg gcgccaatct ctccctgaac gggcaccggt tttcgccgga
     4321 aggccatttg aacagccggg aattgctaag cgttctttct gaaatgagaa acggtaattt
     4381 cagcgcccgc atgccggtcg atctgctggg cctggatgga aaaatatgtg ataccctcaa
     4441 tgagatcata tcgctgaacg aggccctgat gctggaactg accaaggccg gaaacatcat
     4501 cggcaggcag ggaaacctga cccatcgcct ggtattgccc cagatagccc gcggccgctg
     4561 gagcgacgga attgattcca tcaacgaact gatatccaac ctggttcatc cgaccattga
     4621 aatcgcccat gtgatcagct ccgtggccaa gggaaatctt tcggaagaaa tgcacctcca
     4681 gatcggcaac cacaagctcc agggcgaatt tcttcgcatt gcccgggaag tgaacgacat
     4741 ggtgaagcag ctcaaccttt tctccatgga agttacccgc gtggccctgg aagtagggtc
     4801 tgaaggaaag ctcggcggcc aggccaaggt aaaaggcgtg gccggtgtat ggaaggacct
     4861 cacggattcg gtgaaccaga tgggaagtaa tctgacggca caggtaagaa atattgcgga
     4921 ggtcaccacg gcggtggccc gcggggatct ctccagaaaa atcacggtgg atgtaaaggg
     4981 ggagatacag gaat
//
LOCUS       DASYCU010000162         7983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1053658, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000162 DASYCU010000000
VERSION     DASYCU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053658"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1209
                     /locus_tag="VGM24_03230"
     CDS             307..1209
                     /locus_tag="VGM24_03230"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEY4154403.1"
                     /translation="MLAVTILGNNSAIPAFDRHPTAQVITMDDQLFLVDCGEGTQMQM
                     SKYKIRRSRINHIFISHLHGDHYFGLIGLITSMGLLGRQQDLNLYAPVPLKEIFDLQL
                     KVADTQLPFNLHFHPLQQEGLLVRNNRFEVSCFRVFHRIECWGFLFRQIKPLRKLNPV
                     KAREYEIPASFFERLKWGENYQDRNGMIIENSLVTDEAPKPRSYAYSADTAYNPVLAE
                     IVKGVDLLYHETTYLQDLEERATKRFHCTTAQAASIARQAGVNKLLIGHFSSKYEKLD
                     EFEKEARQVFPNTELAVEGVTYRV"
     gene            complement(1225..2190)
                     /locus_tag="VGM24_03235"
     CDS             complement(1225..2190)
                     /locus_tag="VGM24_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY4154404.1"
                     /translation="MKRFQELSHAFLTHFGIKHFPDQPAGLYDAASYMLGVGGKRIRP
                     LLCLMGNELFTDIQPAAWEIAAGIELFHNFTLIHDDIMDKAPLRRGNPTVHMRYNESS
                     ALLAGDVMLLKAYEYFNAADPRYARILIDLLNQTGRQVCEGQQVDMDFEKSAVVSLTD
                     YLHMIELKTAVLLAASLQMGSIIAGAGETDQEHLYAFGKNLGMAFQVQDDWLDAFGDP
                     EKFGKQTGGDIVQNKKTFLLMQALETAGSKQKEELLQLMDYGGPDKVARMLVLFKACG
                     VDRAAETAQKNYRDQAFASLGKISVPEERKAPLKELADQLLQRHF"
     gene            complement(2209..4176)
                     /gene="rnr"
                     /locus_tag="VGM24_03240"
     CDS             complement(2209..4176)
                     /gene="rnr"
                     /locus_tag="VGM24_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY4154405.1"
                     /translation="MGHKHPDKKKKKSTDNLYRGTLEVTRSGMGFVISKTFGQDVLVR
                     PNDFNTAMHGDTVLVKITRGQAGSGRVQGEVEAVVERKQTDFPGHIEMGDGFAFFIAE
                     TARPMPDIYVSRSKLNGAADKDRVIVRITEWTKNKKPQGEVIQVLDESDENDRVMKEI
                     LLENGFPIVFPEEVMIASGKLPEQIPPGEAAKRRDFRDTLSFTIDPADARDFDDAISF
                     RVLKDDLYEVGVHIADVSHYVETGSLLDEEAYQRATSVYLPDRVNPMLPERISNELCS
                     LRPNEDKLTFSAVFTITPKGEIKDYWLGRTIIHSNHRFTYEDVQEIIEKKQGKYETEI
                     LILHDLAQRFRRQRIKKGAINFSSVEVRFKLDEKGKPVGITVKESKESHQLIEEFMLL
                     ANRTVATHVSRIKIKKKAIPFPYRVHDTPDKEKLAPFVLFAKKYGHHFDTKSPEGIAA
                     SFNQMLQDVQGKPEQHVLEQLGIRTMAKAVYSTENIGHYGLGFSNYCHFTSPIRRYPD
                     VLTHRILQEVLDDNIQVRKKLDEQCKYCSERERAAMDSERSANKYKQVEFMKEFVGDV
                     FEGVISGVAAFGYWVETVEHKCEGLVSLNSLMEYDDFRLIEGDYALVGRRSGRSFRMG
                     DKVRIKVVSANLAKRQLDYEWVLSPSPSSKP"
     gene            complement(4183..5262)
                     /gene="lpxK"
                     /locus_tag="VGM24_03245"
     CDS             complement(4183..5262)
                     /gene="lpxK"
                     /locus_tag="VGM24_03245"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY4154406.1"
                     /translation="MINLNYPLLKPVRILLLPFSLIYGLIVYIRNWLFDKKFIQSASF
                     NLPLICVGNLAVGGTGKSPMVEFLIGNLKDRFKVAILSRGYKRKTSGYALATGTTNAL
                     EIGDEPMQFFNKFPDVTVAVGEERIVAIPQLLHDKPDTQVIILDDAFQHRYVKAGFNI
                     LLTDVNNLFTRDWFLPTGDLRDQKKSYLRADLLVVTKCSADLSLAEKRWLIKEINPAS
                     HQKVFFTAIRYGKPYQIVTKEIYIPVKETEVLLVTGIANPAPLKRHLEENFKAYDELA
                     YGDHHIFSIDDLKYILARFKQIQSDHKIIITTEKDAVRLIKFSQQLEGLPLFVLPIQP
                     VFLFHEETEFVRLITTFIQEFPDQA"
     gene            complement(5282..5944)
                     /gene="bioD"
                     /locus_tag="VGM24_03250"
     CDS             complement(5282..5944)
                     /gene="bioD"
                     /locus_tag="VGM24_03250"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY4154407.1"
                     /translation="MRPFFVSGIGTGIGKTVVSALLCETLQADYWKPVQAGFAEGTDS
                     ETVRSLVTNPQTFIHPETYRLQLPASPHIAAREENTRIEFSRIKKHFASMNTGRPLII
                     EGAGGLLVPLNESEMIADLVSLLDARLILVSRNYLGSINHSLLTAEVCRQRQLEVSGW
                     VFNDQFMDYEGEIVSWTGIPAICSVPFMPRQTREGISMEAKRIGDRVRNRLLPATDHP
                     RE"
     gene            complement(5944..6489)
                     /locus_tag="VGM24_03255"
     CDS             complement(5944..6489)
                     /locus_tag="VGM24_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY4154408.1"
                     /translation="MRIIGGALGGRRLNPPAKMPLTRPTTDIAKEGLFNILQSNRNLE
                     GIKTLDLFGGTGCISFELASRGAGDLTIVEKNHQMEAFIKKTAAELGITNLKTYRSDV
                     FRFLNTNSGTQYDFIFAGPPYALEDIDRIPELIVQGHFLKENGWFVLEHTPRNHYEDF
                     PGFATVRNYGTTFFSIFINKH"
     gene            complement(6480..7340)
                     /locus_tag="VGM24_03260"
     CDS             complement(6480..7340)
                     /locus_tag="VGM24_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HEY4154409.1"
                     /translation="MIIRPDFDIHNEDPAAEDPVQCRLLIEMGSSSLTYVLLNVRGMR
                     PAAIKVFQWNAQKAGPGDEVTRDIIDQEQILSQFPANEVFLIYNFPESNLVPEKYFSD
                     DCARPVTQLVYGDLSPDLVLDEKIPWFEFHNVYRVPRKLHYLMQEKYPNARFWHFYSL
                     QLKCYKMFTAKEEPLFLKTYFYPDQMQVMICKRGQLQLIQHFPYQDSKDVLFHLLNCC
                     QQLGMNREEVVLELCGMIEKKSALYEMLDQYFLNIRFDSLEDSIRTTDELLQYPSHFF
                     SSLLKMAICV"
     gene            complement(7404..>7983)
                     /locus_tag="VGM24_03265"
     CDS             complement(7404..>7983)
                     /locus_tag="VGM24_03265"
                     /inference="COORDINATES: protein motif:HMM:NF045274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154410.1"
                     /translation="LSSRIGARQSGKEKMQQYFMAARNYASAALRCDSTSSDANFVMA
                     LALGRLSLIAGTRERIVLARDIKRYAEISIRLDPGNFKPYHILGKWNYEVSDLNVAER
                     SIARLIYGGLPPASLKKSIYYFEKSRSMNPAFLLNYLELARAYHRNNDNKKAFSLLRN
                     LLVLPNMIYDDTRVKATARELLKEWQSRLPAY"
BASE COUNT         1856 a         2094 c         2000 g         2033 t
ORIGIN      
        1 gcgaacttca gccttcgctg aaaaaaatat ttgaccggaa tgaacagtcg ggcagcattc
       61 agatcacctc aacctatagc gaagcggtgg atctggttca tctggaagaa atcgaaagag
      121 aaatgacgga ttagactaaa ttccccggga ttatatacgt tcccccggca catttgttca
      181 ttcgatggct ccggatataa tggaaacgac cgaatcccgt tcctattact atttgtatta
      241 tttttccggt cgggtagcta ctccgtcccg gatgctgtct gcctgatcat acctcaaaat
      301 ctgtttgtgc tggcggtaac tattcttgga aacaattctg caatcccggc tttcgaccgg
      361 catcctacgg cccaggtcat cacgatggat gaccagcttt tcctggtgga ctgcggggaa
      421 ggaacccaga tgcagatgag caagtataag atccgccgga gccggatcaa ccacatcttt
      481 atttcccacc ttcacgggga tcactatttc gggctgatcg ggctgatcac cagcatgggt
      541 ctgctcggac gtcagcagga cctgaacctt tatgcccccg ttcccctgaa agagatcttc
      601 gacctgcagc tgaaggtggc cgatacccag ctccccttta accttcattt ccacccctta
      661 cagcaggaag gactgctggt gagaaataac cgttttgaag taagctgttt ccgggtattt
      721 caccggattg aatgctgggg cttcctcttc cggcagatca aaccgctccg gaaactgaat
      781 cccgttaagg cccgggaata tgagatcccg gcttcttttt ttgaacggct gaaatgggga
      841 gaaaactacc aggaccgaaa cgggatgatc atcgaaaatt cactggtgac ggacgaagcc
      901 ccaaagccca ggtcttatgc ctattcggca gacaccgcct ataatccggt cctggcggag
      961 atcgtcaaag gcgtggatct attatatcat gagacgacct atctgcagga tcttgaagaa
     1021 agggcaacca aaagatttca ttgcactacg gcccaggccg cttccattgc ccggcaggcc
     1081 ggagtgaata agttgctgat cggacacttc agctccaaat atgaaaagct ggatgagttt
     1141 gaaaaggagg cgcgccaggt tttccccaat acggaactgg cggtggaagg agtcacctac
     1201 cgggtctagg aaaccgctca ggtctcaaaa atgacgctgc aggagctgat cggccagttc
     1261 tttcaaaggc gcttttcttt cctccggaac gcttatcttt cccaggcttg cgaatgcctg
     1321 gtcccggtaa tttttttgcg ccgtttccgc agcccggtcc acgccgcagg ccttaaacag
     1381 gaccagcatt ctcgccactt tatccggccc gccatagtcc atcagctgca ggagctcctc
     1441 tttttgtttt gaacccgccg tctcgagcgc ctgcatcagc aggaatgttt ttttgttctg
     1501 aacgatatcc cccccggttt gcttaccgaa tttttccgga tcgccgaagg catcgagcca
     1561 gtcatcctgt acctggaagg ccatacccag attttttccg aaggcataca aatgctcctg
     1621 atcggtttct cctgctccgg ctataatgga acccatctgc aggctggctg ccagcaatac
     1681 ggccgttttg agctcaatca tgtgcaggta gtccgtcagg ctgaccactg ccgatttttc
     1741 aaaatccata tccacctgct ggccttcaca aacctgccgc ccggtctggt ttaacagatc
     1801 aataaggatc cgggcatagc gcggatcggc cgcattgaaa tattcatacg cttttaacag
     1861 catcacgtcc cctgccagca aggcagatga ttcattgtag cgcatatgta cggttgggtt
     1921 gccgcgacgg agcggcgctt tatccatgat atcgtcgtga atgagcgtaa aattgtgaaa
     1981 gagctcgatg ccggcagcta tttcccaggc ggcgggctga atgtccgtaa acagttcatt
     2041 acccattaaa cagagcaggg gccggatcct tttcccgccc acccccagca tataggatgc
     2101 ggcatcatac aatccggctg gctgatccgg aaaatgtttg atgccgaaat gggttaaaaa
     2161 agcatgcgat aattcctgga atcttttcat gtttacgagt aatgatcgtt agggtttgga
     2221 tgacggggat ggggagagca cccattcata atccagctgg cgcttggcca ggttcgccga
     2281 gaccaccttg atccggacct tgtcgcccat gcggaaactc cttccgctgc ggcggccgac
     2341 cagcgcatag tccccttcaa tgagacggaa atcatcgtat tccatcaggc tgttcaggct
     2401 gaccaatcct tcacatttgt gttccaccgt ctcaacccaa tatccaaagg cggccacgcc
     2461 gctgatcacc ccttcaaaca catccccgac aaattccttc atgaattcca cctgtttgta
     2521 tttattcgcc gaccgttcac tgtccattgc cgccctttcc ctttcgctgc agtatttgca
     2581 ttgctcatcg agttttttcc ggacctgtat attgtcgtcc agcacttcct gcaggatccg
     2641 gtgcgtcagc acgtccggat aacgccgtat cggcgaggta aaatgacagt aattcgaaaa
     2701 tccgagcccg tagtgcccga tattttcggt tgaataaacc gctttggcca tcgtgcggat
     2761 acccagttgt tccagcacat gctgttccgg ttttccctgt acgtcctgca acatctggtt
     2821 aaaggaggcc gcaattcctt ccggcgattt ggtatcgaaa tgatggccgt attttttggc
     2881 aaacaatacg aacggagcga gcttttcctt atccggcgta tcatgcaccc ggtatggaaa
     2941 ggggatcgct ttctttttta tttttatcct ggaaacatgc gtggcgacgg tccggttggc
     3001 cagcaacatg aattcttcga tcagctggtg tgattcctta ctttccttga cggtaatacc
     3061 aaccggcttc cctttttcat ccagtttaaa ccgcacttcc acggaagaaa aattgatcgc
     3121 cccttttttg atccgctggc ggcggaaacg ctgggccaga tcgtgcagga tcaaaatctc
     3181 cgtttcatat ttcccctgtt tcttctctat gatctcctga acgtcctcgt acgtaaagcg
     3241 atggttggaa tgtatgatgg ttctccccag ccagtaatcc tttatctctc ccttcggggt
     3301 gatcgtaaat acggcggaaa aagtcagttt atcttcattg ggcctcaggg agcaaagctc
     3361 gttggaaatt ctttccggca gcatcggatt cacccggtcc ggcaggtaaa cggaagtggc
     3421 gcgctgatag gcttcttcgt cgagcaggga accggtttcc acgtaatggc tcacatccgc
     3481 aatgtgcaca cccacttcgt acagatcgtc cttcagcacg cggaaggaaa tcgcatcatc
     3541 aaaatccctg gcatccgccg gatcgatcgt gaagctgagg gtatctctga aatctcttct
     3601 tttagcggct tcgcccggcg ggatctgttc cggaagtttc ccggaggcga tcatcacttc
     3661 ttccggaaaa acgatgggga atccgttttc cagcaggatc tccttcatca cccggtcatt
     3721 ctcatccgat tcatccagaa cctggatgac ctcaccctgc ggtttcttgt ttttggtcca
     3781 ttcggtgatc cgcacgatca cgcggtcctt atccgccgcc ccgttcagtt tcgaccggga
     3841 gacatagata tccggcatgg gccgcgccgt ttcggcaata aaaaaggcaa atccgtcccc
     3901 catttcaata tgtcccggga aatcggtctg ctttctttcg acgacagctt ccacttcccc
     3961 ctgaacgcgg ccgctcccgg cctgcccccg ggtgatcttc accagtacgg tatccccgtg
     4021 catggcggta ttgaaatcat tgggacgtac cagcacgtcc tggccgaagg ttttggaaat
     4081 cacaaaaccc attcctgagc gggtgacctc cagcgtacct ctgtataagt tgtctgttga
     4141 ttttttcttt tttttatccg gatgtttatg acccatagat gattaagctt gatcaggaaa
     4201 ttcctgaatg aatgtggtaa ttaggcggac aaattccgtt tcttcatgaa acaggaagac
     4261 cggctggatc ggcagtacaa aaagcggcag cccctccaac tgctgggaaa atttgatcag
     4321 ccggaccgcg tccttttccg tcgttatgat tattttatga tccgactgga tctgtttaaa
     4381 acgcgcaagg atatatttca ggtcgtcaat gctgaagatg tggtggtcgc cataggccag
     4441 ctcgtcgtag gccttaaaat tttcttccag gtgccttttc agcggggccg gattcgcaat
     4501 gccggttacc agcaatactt ctgtttcctt taccggaata tagatttctt ttgttacgat
     4561 ctggtacggt tttccgtagc ggatggcggt aaaaaagact ttctgatggg aagcgggatt
     4621 gatctctttt atcagccagc gtttctcggc cagggaaaga tccgcggaac atttggtcac
     4681 cacgagaagg tccgcccgga gataactttt tttctgatcc cgcaggtcgc cggtgggcag
     4741 gaaccagtcg cgcgtaaaca gattattcac atcggtaagc aaaatattaa atcccgcctt
     4801 tacataacgg tgctgaaacg catcatcgag aatgatcacc tgcgtatcgg gtttatcatg
     4861 caggagttgt ggaatggcca cgatcctttc ttcgcccacg gcaacagtga cgtcgggaaa
     4921 tttattgaag aactgcatgg gttcatcccc gatctccagg gcgttggtgg ttccggtggc
     4981 cagcgcatat ccgctggtct tccgcttata gccccggctc aggatggcca ctttaaagcg
     5041 gtctttcaga tttccgatca ggaattccac catgggcgat ttgccggtgc cgccaaccgc
     5101 cagattaccc acgcagatca aaggcaggtt gaaagaagcg gactgaatga atttcttgtc
     5161 gaagagccag ttgcggatat agacgatcaa tccatagatc agtgaaaatg ggagcagcag
     5221 gatcctgacg ggctttaata agggataatt cagattgatc ataacggcca gcaaaggtaa
     5281 ctcattcccg gggatggtct gtagcgggaa gtaaccggtt tctgacccgg tctccgatcc
     5341 gctttgcttc cattgatatt ccttccctgg tctgccgggg cataaaaggc accgagcaga
     5401 tggccggaat tcccgtccag gaaacgatct ctccctcgta atccataaac tggtcgttga
     5461 acacccagcc ggaaacctcc agctgccgct gcctgcagac ctccgcggta agcagggaat
     5521 gattgatgct tccgagataa tttcggctca ccaggatcag cctggcatcc agcaggctga
     5581 ccaggtcagc gatcatttcc gattcattga ggggaaccag cagtcctccc gcgccttcga
     5641 tgatcagcgg acggccggtg ttcattgacg cgaaatgttt ttttatccgg ctgaactcta
     5701 tccgggtatt ctcctcacgg gcagcaatat gcggagaagc cggcagctgg aggcgatagg
     5761 tttccggatg aatgaaggtc tgggggttag tgaccaggga acggacggtt tcactgtcgg
     5821 ttccctccgc gaatcccgcc tgtaccggct tccagtagtc tgcctgcagg gtttcgcaga
     5881 gcagggcaga tacaactgtt ttgccgatcc ccgttccaat gccgctgacg aaaaatggcc
     5941 gcatcagtgc ttattgataa agatggaaaa aaaggtggtg ccgtaatttc gtaccgtggc
     6001 aaacccggga aaatcttcat aatggttgcg gggagtgtgc tccagcacaa accagccgtt
     6061 ttccttcagg aaatgaccct gcacaatgag ctcggggatc cggtcaatgt cttccagcgc
     6121 ataaggcgga ccggcaaaaa taaaatcgta ctgcgttccc gaattggtat ttaaaaaacg
     6181 gaagacatcg gagcggtaag ttttcaggtt ggttatcccg agttccgccg ccgttttttt
     6241 gataaaggct tccatctgat ggtttttttc cacaatggtc agatcgccgg cgccgcggga
     6301 ggccagttca aaactgatgc agcctgttcc tccgaataag tcgagcgttt ttattccctc
     6361 caggttccgg tttgactgaa ggatattgaa caacccttct ttagcaatat ccgtggtcgg
     6421 acgggtcagc ggcatttttg ccggagggtt tagccttctg ccgcccaatg cgccaccgat
     6481 tatacgcata tcgccatttt cagtaacgaa gagaaaaaat gacttgggta ttgaagcaat
     6541 tcatctgtcg tacggatact gtcttccagg ctgtcgaacc ggatattcag aaaatactgg
     6601 tcgagcatct cgtataaagc ggattttttt tcgatcatac cgcaaagctc caggaccact
     6661 tcttcgcggt tcatgcccag ctgctgacaa caattgagca ggtgaaacaa cacatctttt
     6721 gagtcctggt aagggaaatg ctgaatgagc tgcaattgcc cgcgcttgca gatcatgacc
     6781 tgcatctgat cgggatagaa ataggttttc aggaacaggg gttcttcttt tgccgtgaac
     6841 atcttatagc atttcagctg caggctgtaa aaatgccaga accgggcatt cggatatttc
     6901 tcctgcatca ggtagtgcag cttccgggga acgcggtaaa cattatggaa ttcgaaccag
     6961 ggaatttttt cgtccaggac caggtccggg ctcaggtcgc catagaccag ctgggtgacc
     7021 ggtctggcgc agtcgtccga aaaatatttc tcgggcacca ggttgctctc cgggaaatta
     7081 tagatgagga aaacctcgtt ggccgggaat tgggagagga tctgttcctg gtcaatgatg
     7141 tcccgggtca cttcgtcacc cggaccggcc ttctgcgcgt tccactgaaa taccttgatc
     7201 gcggcaggcc gcatgccccg cacattgagc agcacgtagg tgagcgagga ggagcccatc
     7261 tcaatgagca gccggcattg aaccggatcc tctgcggcgg gatcctcatt gtgtatatca
     7321 aaatcgggtc tgatgatcat aaagtgagcg caataatact acaattctgc gactgaactg
     7381 ccagccggta aagcagaatg tgctcagtaa gcaggcaacc gggattgcca ttctttgagc
     7441 aattcccggg ccgtggcctt cacccgcgta tcatcataga tcatattggg aagaaccaaa
     7501 agattgcgca gcagggaaaa cgctttttta ttgtcattat tccggtgata agcccgggcc
     7561 agttcgagat aattaaggag gaaggccggg ttcatggacc ggctcttttc gaaataataa
     7621 atggattttt ttaaactcgc cggaggcagg ccaccgtaaa tcagccgggc gatcgatctt
     7681 tccgcaacat tcaggtcgct tacttcatag ttccactttc ccagtatatg ataaggtttg
     7741 aaattgcccg gatccagccg gatagagatt tcagcatatc gtttaatgtc cctggccagt
     7801 acaatccttt ctcttgttcc ggcaatcagg gaaagccgtc cgagcgccag ggccattaca
     7861 aaattggcat cggaagaggt ggaatcacat cgaagcgcag cggacgcata gtttctggcc
     7921 gccataaagt attgctgcat tttttctttc ccggattgcc gggcgcctat ccggctggag
     7981 agt
//
LOCUS       DASYCU010000163         5352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2071421, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000163 DASYCU010000000
VERSION     DASYCU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071421"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGM24_03270"
     CDS             <1..533
                     /locus_tag="VGM24_03270"
                     /inference="COORDINATES: protein motif:HMM:NF026080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HEY4154411.1"
                     /translation="DAYTSLPKIEGRYEFSEIIQLDSTYKKDDLYRNAKLFFTDAFKN
                     AKEVLQYDDRQEGKVIGKGNLQNNAYQVVFLAVYSEKRTTNFTLEIFCKDGKYRYRIY
                     GIDSDFATTVSGGNTPDRITTGSVDLDEAYNETVKGSLKKLNRKLFFDTVIGLNDMRE
                     LLKHYMGQKQAKDNDF"
     gene            678..1142
                     /locus_tag="VGM24_03275"
     CDS             678..1142
                     /locus_tag="VGM24_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY4154412.1"
                     /translation="MAQLFNVRVYGILTGKNMELLVSDELIRGSYITKFPGGGLEFGE
                     GTRDCLKREFLEEMDLKVKVTDHLYTTDFFQMSAFNPEHQIISVYYRVEALEEIRAPL
                     RTAIFDFDEVQMATYRRTGETETFRFIKREDFNPERLTLPIDKVVAEMLTRV"
     gene            complement(1139..1639)
                     /locus_tag="VGM24_03280"
     CDS             complement(1139..1639)
                     /locus_tag="VGM24_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4154413.1"
                     /translation="MQKDNQIENSANPPVPLDEKDRAILRLLQANAKMTVREIAANVH
                     LSTTPVHERIKRMEDEGVIVQYATLVNHARIKKGLMAICYISLKEHNKRSGAKFIKLI
                     QDFPEVIEFYIISGAFDFMLKVVAESMDHYYDFHVNKLGQVEYIGQVQSTFVMGVIKQ
                     TQPVIS"
     gene            1761..3086
                     /gene="ahcY"
                     /locus_tag="VGM24_03285"
     CDS             1761..3086
                     /gene="ahcY"
                     /locus_tag="VGM24_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY4154414.1"
                     /translation="MSTKAQNIDLKLPYRVKDLSLAEWGRKEIRLAEAEMPGLMSIRA
                     EYGKQKPLKGARIAGCLHMTIQTAVLIETLIELGAEVRWSSCNIFSTQDHAAAAIAAT
                     GVGVFAWKGQSLEEADWCIEQTLFFGNTDHPLNMILDDGGDLTNMVLDKYPELVQHLK
                     GISEETTTGVHRLYERVAKGTLPVPAINVNDSVTKSKFDNKYGCKESLVDAIRRATDV
                     MLAGKVAVVGGYGDVGKGSAHSLQGAGCRVMVTEIDPICALQAAMDGFEVKKMADAVK
                     EADIVVTASGCRDLITEKHFRSLKDKAIVCNIGHFDIEIDMAWLNKSYGHTKDTIKPQ
                     VDLYTIEGKDVIVLAEGRLVNLGCATGHPSFVMSNSFSNQTLAQIELWQNHSRYENKV
                     YVLPKHLDEKVARLHLGKIGVELDELTEAQSDYLGIPKAGPYKADHYRY"
     gene            complement(3154..4095)
                     /locus_tag="VGM24_03290"
     CDS             complement(3154..4095)
                     /locus_tag="VGM24_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4154415.1"
                     /translation="MPYIQTAKSSIAPPVNLYYEDTLHGKPVIFIHGWPLSGRMWEYQ
                     LNELPKYGLRCITYDRRGFGKSDKPWGSYDYDTLAADLRTVIEELNLEEVTLVGFSRG
                     GGEVARYIGKYGTEKVHQVVLISTVLPFLLKTEDNPEGVPQEMFADFITRIREDRPAF
                     FTHFANLFYGVNMLNKSVSPDFLNWNQSLVMQASPKATTECIHSFSETDFRNDISKIN
                     VPALIIHGDRDKIVPIEISSKKTAQLMPEAVFKIYENEPHGLFYTQKDRLNQDLLDFI
                     VGSDTREEPLYDESEPGAKDPLWQDSSTFPDSTFPIL"
     gene            complement(4199..5119)
                     /locus_tag="VGM24_03295"
     CDS             complement(4199..5119)
                     /locus_tag="VGM24_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY4154416.1"
                     /translation="MSTSRRNFLKTGGLSVAALAIGSHKLFAAIPPEIYLGIQLYSVR
                     DDMDKDPSGTLKKLADIGYRYVEHANYHRRKFYGYSPADFKKLLEGYGLHMPSGHVAM
                     TPKDYDFSKNDFSDNWKYTVEDAAAAGQQYLISPWLDESLRKTMSDFKAFMGVFNKSG
                     ELCKKSGMKFGYHNHDFEFSTKLDGKELYDLIMENTDPSLVAQQLDMGNLYNGGAKAI
                     DIATKYPGRFELMHVKDEIPSTGKANNGGEKYESTILGKGIVGTKEICDIGRNSGGTK
                     YFIIEQESYQDKTPVESALEDYKIMKGWGY"
BASE COUNT         1393 a         1183 c         1311 g         1465 t
ORIGIN      
        1 aagacgccta tacctccctt cctaaaatag aagggcgtta tgaattttct gaaattatcc
       61 aattggattc aacatataag aaagatgatt tgtaccggaa tgcaaagtta tttttcactg
      121 atgcgtttaa aaatgcaaag gaggttcttc agtatgatga ccgacaggaa ggtaaggtaa
      181 tcggtaaggg gaatcttcag aataacgcct atcaagttgt ctttcttgct gtgtattccg
      241 aaaagaggac gacaaatttt actcttgaaa ttttttgcaa ggatggcaaa tacagatacc
      301 gaatatatgg gatagattca gattttgcaa ctactgtaag tggtggcaat acccctgatc
      361 gcattacgac tggatctgtt gatttagatg aagcttacaa tgaaacagtt aaaggaagtc
      421 ttaaaaaatt aaatagaaaa ctattttttg atactgtgat tggtctgaat gacatgaggg
      481 aattacttaa acattatatg ggccaaaagc aagcaaaaga taacgatttt tagcactttt
      541 taaatagaaa aacgttagac gaaaatatta acttactcaa aatcaattga ttaaatccct
      601 atgcgggatc tgctcggaac ctcgcagccc gcaatccgta ttgcgggttt ttacgttctt
      661 ataaagaatg cgaatacatg gcccagctgt ttaatgtaag agtatatggt atcctgaccg
      721 gaaagaatat ggaattgctg gtttctgacg aactgatccg gggaagctat atcaccaaat
      781 ttcccggagg cggattggaa ttcggggaag gaactaggga ttgcctgaaa cgggaattcc
      841 tggaagagat ggacctgaag gtaaaagtga cggatcattt gtataccacg gatttttttc
      901 agatgagtgc tttcaatccg gagcaccaga tcatttccgt ttattaccgc gtggaagccc
      961 tggaagagat ccgggctccc ctcagaacgg ccatattcga ttttgatgaa gtacaaatgg
     1021 ccacttaccg gaggaccggc gaaacggaaa ccttccggtt cataaaacgg gaggatttca
     1081 atcccgaaag gctaacactg cccattgata aagtagtggc cgaaatgctg acgcgggttt
     1141 aactgatgac cggttgggtc tgcttgatca cccccattac aaaagtgctc tgcacctgcc
     1201 cgatatattc cacctggccc agtttattta cgtggaagtc gtaatagtgg tccatgctct
     1261 ccgcaaccac cttaagcatg aaatcaaatg ctccggaaat gatgtaaaat tcaatcactt
     1321 ccggaaaatc ctggatgagc ttaataaatt ttgctccgga gcgtttgttg tgttccttca
     1381 gggaaatata gcagatggcc atcagtccct tctttatccg cgcatggttg accagggtgg
     1441 cgtattgaac aatgacccct tcgtcctcca tccgtttgat ccgctcatgg accggcgtgg
     1501 tgctgaggtg aacattcgcc gcgatttccc ggacggtcat cttcgcgttg gcttgtaaca
     1561 gccgtaatat agcccggtct ttttcatcca ggggtaccgg cgggttagct gaattttcta
     1621 tttgattgtc tttttgcatg aagtaatgtt tatttattct ttaaattacc ttaaaactat
     1681 tactttattc caaatttact cacattatca gtatttttgt tctacgttct tatctttgct
     1741 ctcattaaat tatttaagcc atgtctacaa aagcacaaaa tattgatctg aagctcccct
     1801 accgggtgaa agacctgagt ctggctgaat ggggccgtaa ggaaatccgg ctggcggaag
     1861 cagaaatgcc cggactgatg tccatccgcg ctgaatatgg caagcaaaag ccattaaaag
     1921 gggcccggat cgccggttgt ctccacatga ccattcaaac ggcggttctt attgaaacgc
     1981 tgatcgagct cggcgccgaa gtgcgctgga gctcctgcaa tattttctca acacaggatc
     2041 atgctgcggc tgccatagcg gccaccgggg tgggcgtttt tgcctggaaa gggcaatccc
     2101 tggaagaggc ggactggtgc attgagcaga ccctgttttt cggaaacacc gatcatccgt
     2161 tgaatatgat cctggacgac ggcggcgatc tgaccaatat ggtgctcgat aaatatccgg
     2221 aactggttca gcacctgaag ggtatttctg aagaaaccac cacgggggtt catcgcttat
     2281 atgaaagggt ggcaaaagga accctgccgg taccggccat caatgtgaat gattcggtca
     2341 cgaaatccaa attcgataat aaatacggat gcaaggaaag cctggtggat gcaatccgcc
     2401 gcgcaacgga tgtcatgctg gccgggaagg ttgccgttgt tggagggtat ggtgatgtgg
     2461 gtaaaggttc tgcccattcc ctgcagggag caggctgccg ggtgatggta accgaaatcg
     2521 atccgatctg cgccttgcag gcggctatgg acggatttga agtgaagaaa atggccgacg
     2581 cggtaaaaga agcggacatt gtggtaacgg cttccggatg ccgggacctg atcaccgaaa
     2641 agcatttccg ttcattgaag gacaaggcga ttgtatgtaa tatcgggcat ttcgatattg
     2701 agatcgacat ggcctggctg aataaaagct atggtcatac caaagacacg atcaaaccac
     2761 aggtggattt atataccatc gaggggaaag acgtgatcgt tctggcggag ggacgtttgg
     2821 tgaatctggg ttgcgccacc ggtcacccga gttttgtcat gagtaattca ttcagcaatc
     2881 aaacgcttgc ccagattgag ctatggcaga accatagccg gtatgaaaac aaagtatatg
     2941 tgctgccgaa acacctggat gaaaaagtag cccgcctgca tcttggcaag atcggtgttg
     3001 aactcgatga attgaccgag gcgcaaagtg attaccttgg gattccgaaa gcaggacctt
     3061 acaaggcaga tcattacaga tattgattcc cggagaagaa attgcgggat gataaggcca
     3121 cgcagaaaca gatcttctgc gtggctttac tttttataat atcgggaagg tagaatcagg
     3181 aaacgtggac gaatcctgcc agagcgggtc tttcgccccg ggttcgcttt catcgtacag
     3241 cggttcttcc cgggtatccg agccgacgat aaaatcgagc aggtcctgat ttaacctgtc
     3301 tttttgcgta taaaaaagac cgtgtggttc attttcataa atcttgaaaa cggcttccgg
     3361 catcagctgt gctgtttttt tactgctgat ctctatggga acgattttat cccggtcccc
     3421 gtgaatgatc agggccggca catttatttt tgaaatatca ttccggaaat ccgtttctga
     3481 aaaggagtgg atgcattctg tcgtcgcctt tggagaagcc tgcatcacca gggactgatt
     3541 ccagttgagg aaatcgggac ttacggattt attgagcatg tttaccccgt aaaacaggtt
     3601 cgcaaaatga gtgaaaaagg ccggccggtc ctcccggatc cgggtaataa aatccgcaaa
     3661 catttcctga gggacccctt ccggattatc ttccgttttt aacaggaaag ggagcaccgt
     3721 actgatcagc acaacctggt ggaccttttc ggttccatat tttccgatat agcgggccac
     3781 ttccccaccc cccctggaaa agccaaccag ggtcacttcc tccagattca attcctcaat
     3841 gaccgtccga aggtcggccg cgagcgtgtc gtaatcataa cttccccagg gcttatccga
     3901 tttaccaaat ccgcgccggt cgtaggtaat gcagcggaga ccgtacttgg gaagttcatt
     3961 cagctggtat tcccacatgc ggccactgag cggccagcca tgtatgaata tcactggttt
     4021 cccgtgaagc gtatcttcat aatacaggtt cacgggcggc gcaatggaac tttttgctgt
     4081 ctgtatgtag ggcataaaat tgattttaat gagatcttat taaaaccgat caaatgcttt
     4141 gccagctttt ttattcagcg cttttttgag ggggtcgggt gccggaccag gggtaagatc
     4201 aatatcccca gcctttcatg atcttataat cttccagggc gctttccacc ggcgttttat
     4261 cctggtagga ttcctgttca atgataaaat atttggttcc tccggagttc ctgccgatat
     4321 cgcagatttc tttcgttccc acaatgcctt ttccaagaat ggtgctttcg tatttttcac
     4381 ctccgttgtt tgccttcccg gtcgacggga tttcatcctt aacatgcatc aattcaaagc
     4441 ggcccggata tttggtcgca atatcgattg ccttcgctcc tccattgtaa agattgccca
     4501 tatcaagctg ctgtgccacc agggaaggat ctgtattttc catgatcagg tcatataatt
     4561 cctttccgtc cagtttggtg ctgaattcaa aatcatggtt gtgataaccg aatttcattc
     4621 ccgatttttt acaaagctcc ccggacttgt taaatacgcc cataaatgct ttgaaatcac
     4681 tcatggtttt acgcaggctt tcatccagcc agggagaaat caggtactgc tgaccggcgg
     4741 ctgcggcatc ttcgacggta tatttccaat tgtccgaaaa gtcatttttt gagaagtcgt
     4801 agtcctttgg cgtcatggcc acatgaccgc tgggcatatg gaggccatag ccttccagca
     4861 attttttgaa atctgcgggt gaatatccgt aaaacttccg gcgatggtaa ttcgcatgct
     4921 ccacataacg gtaaccgata tccgctaact ttttcagcgt gccggaagga tccttgtcca
     4981 tgtcgtcccg gacggaatac aattggatcc cgaggtaaat ttcaggtggg atggcggcaa
     5041 ataatttatg ggagccaatg gccagggcag caacggaaag tccgccggtt ttcaaaaaat
     5101 tcctgcgtga agtactcatg acaattgttt ttgtgaaaga tcttttaaag ttatcggatt
     5161 tggccgtttt tgggaaaaaa gttttttgcg cgaaatgccg gtgtcgggaa tccggtgttt
     5221 tccctgtccg gtgcgctgga tgggattcag cgctccgtgt tcagcgtacc gctttccggc
     5281 ctttgtgttc cgggtttaaa cgtaaaatgc ttttgtgtga ggtaactgaa ggatacgacg
     5341 atggaggtgg tg
//
LOCUS       DASYCU010000164         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3519547, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000164 DASYCU010000000
VERSION     DASYCU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519547"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VGM24_03300"
     CDS             <1..907
                     /locus_tag="VGM24_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154417.1"
                     /translation="TLANRIALYKLILDTRLKTVAIAADYTSAAAGTPQQAGELFTRD
                     IKETGDLIQAYTASRETFRQAWLKENQHYWLDSALTAYDKKINRLTRLRETLKQNKTF
                     AANGHSLSPAGSSGLLIRESTNYYFQNWMLAGPFLLSDQDKFPDYLYSDNPEYNKPPS
                     PGDFTQFRGKLYRWKKFASTDGGIIDPGGNYSRLQPVMFYAYCSITSDSAKQVPAFIR
                     SDAALEIYCNGRKLSEAFSEADAAKEIREDLPLKAGVNQILLKFRETGEPGWTFTFRL
                     RDHLAITNHKHKYQLNAKTVSYDEE"
     gene            894..2144
                     /locus_tag="VGM24_03305"
     CDS             894..2144
                     /locus_tag="VGM24_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154418.1"
                     /translation="MTKSKIGIYFIALWLLMPCPGGLHAQVKKEDITRAINETARYVA
                     DVILDQEGKSRCDYNVMEGKWYPYEVPWHTGQAVNALLAAYKQTGDKYYLDAARRGGN
                     YWISMEVKDTPLALKGMVRGIHGDVLGDDFIVFATVSDGTPGIYELSRVTKDPRYAKV
                     ATSAASWMLEHMYYPEQGVCYDNVDAKTGEVLKEYSPFWKDKKEQKLFDVSRPNTEGY
                     LFKDAFEFSHEKKFKDAFINLCNSLVEKQGPEGVWMEFMPNFADQHSFHPRFSLWYAE
                     SLIEAYKLTGDKKYLESAAKTARVFVKAQQANGTIFYDNYTDGRPPDKGSATGSAVAF
                     AGIVWIELSKEGYKEFDAPIEKSARWLLKNRYAQNLPDPNLRGAVLETRTRMKKDRLW
                     ITNRDIGTSFAVRFFADYLNFKYK"
     gene            2214..>2650
                     /locus_tag="VGM24_03310"
     CDS             2214..>2650
                     /locus_tag="VGM24_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4154419.1"
                     /translation="MKKSMKRLCLLTSLIIPLFSAVLAQQTSGNIRGQVTDPEGNALP
                     GASVMIKGSTRAVVTNLSGNFEILGVKAGNCTLVVHYMGYKPAETDLQVVSGQTANKN
                     FVLNINSVNLTSVTVSALLEGQQKALNQQRNADNIKQVVSADL"
BASE COUNT          730 a          650 c          681 g          589 t
ORIGIN      
        1 cacactggcc aaccggatcg cattgtataa actgatcctg gatacccggc tgaaaacggt
       61 ggctatcgcc gctgactata cgagcgcagc cgcgggaacg ccgcagcagg ccggagaatt
      121 attcacccgc gatataaagg agaccgggga tctgatacaa gcatataccg cttcaaggga
      181 aacattcaga caggcctggc tgaaggaaaa tcagcattac tggctggaca gcgcgctgac
      241 ggcttatgat aaaaagataa accgcctgac ccggctaagg gaaacgctga aacagaacaa
      301 aacattcgca gcaaatggcc attctctttc tcctgccgga tcatccggat tgctgatccg
      361 ggaaagcacg aattattatt tccagaactg gatgctggcc gggccgttcc tgctgtcgga
      421 ccaggacaaa tttccggact atctgtattc ggacaatccg gaatataata agccgccatc
      481 accgggagat tttacgcagt ttagggggaa attataccgg tggaagaaat tcgcttcaac
      541 ggacggaggg atcatcgatc ccggtggaaa ttatagccgg ctgcaaccgg tcatgttcta
      601 tgcctattgc tccatcacat cggattcggc caaacaggta ccggccttta tccgttccga
      661 tgcggcgctg gaaatttatt gtaacggccg gaaactttca gaagcttttt ctgaagcgga
      721 tgcagctaag gaaatcaggg aagaccttcc cctgaaggcc ggtgtcaacc agatcctgct
      781 aaaattcaga gaaaccggcg aacccggttg gacctttact ttccgcctcc gggatcacct
      841 ggccattacg aatcacaaac ataagtatca actaaatgcg aaaacggtat cctatgacga
      901 agagtaagat cgggatctat tttattgcct tgtggctgct tatgccatgc cccggcgggt
      961 tgcatgcgca ggttaaaaag gaagacatta ccagggcgat caatgaaact gcccggtatg
     1021 tagccgatgt tatcctcgat caggaaggaa aatcccggtg cgactataac gtgatggaag
     1081 ggaaatggta tccatacgaa gtgccctggc ataccgggca ggcagtcaat gccctgctcg
     1141 cagcctataa gcaaactggt gacaaatatt atctcgatgc agccaggcgg ggcggcaatt
     1201 actggatcag catggaggta aaggatacac cgcttgccct gaaggggatg gtgaggggaa
     1261 tccatgggga tgtgctgggc gacgacttca tcgtgtttgc cacggtatcc gatggaactc
     1321 ccggcatcta cgaattatcc cgggtgacca aagatccccg gtacgccaaa gtagccactt
     1381 cggctgcctc ctggatgctg gagcatatgt actatcccga acagggagtc tgttatgata
     1441 atgtggatgc gaaaacggga gaagtattga aagagtacag tccgttctgg aaagataaaa
     1501 aagaacaaaa actctttgac gtatcgcgtc cgaatacgga agggtatctg ttcaaagacg
     1561 cctttgaatt ttcccacgag aagaaattca aagacgcgtt tattaatttg tgcaatagcc
     1621 tggtggagaa gcaggggccg gaaggcgtgt ggatggagtt catgcccaat ttcgcagacc
     1681 agcattcttt tcatccgcgt ttttcgctct ggtatgccga atcgcttatt gaagcctata
     1741 aattaaccgg cgataagaaa tacctggaat ctgccgcgaa aacggcccgc gtttttgtga
     1801 aggcacagca ggcaaacgga accatctttt acgacaatta cacggacggg aggccgcccg
     1861 ataagggatc tgccaccggc tccgccgtcg cttttgccgg gatcgtgtgg atagaacttt
     1921 caaaggaagg gtacaaggaa tttgatgctc ccattgaaaa gtcggcccgc tggttactga
     1981 agaaccgcta tgcacagaac cttcccgatc ccaacctgcg gggtgcggtg ctggaaacca
     2041 ggacccggat gaagaaggac cggctatgga tcaccaaccg cgacatcgga acttcttttg
     2101 cggtccgttt tttcgcggac tacctgaatt ttaaatacaa ataatcttta ttgaattttt
     2161 aaaccatcct caccgtgtct gtcatcagtt tttataacca aaaaattctg caaatgaaaa
     2221 aaagtatgaa aagattatgc ctgttgactt cgctgatcat acccctcttt tcggctgttc
     2281 tggcccagca aaccagcggt aatattagag ggcaggtgac ggatccggaa ggcaacgccc
     2341 ttcccggtgc gtccgttatg atcaaaggca gcacccgcgc cgtagtcacc aacctctcag
     2401 gtaattttga aatactcgga gtaaaagcag ggaactgtac gctggttgtg cactacatgg
     2461 gttacaaacc ggccgaaacc gatcttcagg tggtatccgg ccagacggcc aacaaaaatt
     2521 tcgtgctcaa cattaacagc gtgaacctga ccagcgtaac ggtatccgcc ttactcgaag
     2581 gccagcagaa agcgcttaac cagcaacgga atgcggacaa tatcaaacaa gtcgtatcgg
     2641 ccgacctgat
//
LOCUS       DASYCU010000165         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2649912, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000165 DASYCU010000000
VERSION     DASYCU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649912"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VGM24_03315"
     CDS             complement(<1..567)
                     /locus_tag="VGM24_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154420.1"
                     /translation="MRGFVLFTACLFIPFATLQAQDDPVFRKHDPKGDPNINPFLNNQ
                     INPDSNLAINPSVNWNMNPQKDNEVNPSRNSAINPMINLQLNPQTNETLNPVFMKSLQ
                     PSYKTWDGMFIFNDSNKLFGYISIAAQNVMISFDNKGTWNGYFVKAGRLIYNYFTVPG
                     IWTGMYLCYDNASGYNLFDKNGKWTGIHV"
     gene            complement(712..1641)
                     /gene="mdh"
                     /locus_tag="VGM24_03320"
     CDS             complement(712..1641)
                     /gene="mdh"
                     /locus_tag="VGM24_03320"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY4154421.1"
                     /translation="MKVTVVGAGAVGATCADNIARKELAEELVLLDIKEGFAEGKAQD
                     MMQSATLLGFDTRITGSTNDYSKTAGSNVVVITSGLPRKPGMTREELIGTNAGIVKTV
                     TENILKYSPDAVLIIISNPMDTMTYLTLQTSGLPKNRIIGMGGTLDSSRFKYQLSQHL
                     GCSPADVNALVIGGHGDTTMIPLIRFATWNSIPVTTLLSEEKQKQIVADTMVGGATLT
                     KLIGTSAWYAPGAAGAALVESIVRDEKKLFTCCVALNGEYGQKDICLGVPVTIGKNGW
                     EKIVSFDLNAGEKALFEKSAAAVRGMNDVLRTL"
     gene            complement(1762..2274)
                     /locus_tag="VGM24_03325"
     CDS             complement(1762..2274)
                     /locus_tag="VGM24_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154422.1"
                     /translation="MKRVLILIVSLFFLLNLQAQTAVSAEPAYVRNPAFPPIRLLKVD
                     SVHYITRDDIKKNRRILLMFFSPECEHCKHQISDILADFSKFKDIEIVMATFQPFDEM
                     KSFYSYYRLGDHPNIRIGRDEKYFLPPYYKMQNLPYLALYDKKGQYITHFEGNQKVET
                     ILNGFSSNDK"
     gene            2450..3016
                     /gene="efp"
                     /locus_tag="VGM24_03330"
     CDS             2450..3016
                     /gene="efp"
                     /locus_tag="VGM24_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY4154423.1"
                     /translation="MANTADISRGLIIKLDGSLYSVIDFGENKTARAAAKVWAKLKGV
                     DNSRSIEKTWNSGDTIYPVRVEKKDYQYLYKDDTGYNFMDTETFEQIALQENLVDAPQ
                     FLKEGQQVTVLMNTETDLPMGAELPDKIVLLVTYSEPGLKGDTATRTLKPAKVETGAT
                     VNVPLFVNEGELIRVNTKTGEYVERVKE"
     gene            3060..3545
                     /gene="accB"
                     /locus_tag="VGM24_03335"
     CDS             3060..3545
                     /gene="accB"
                     /locus_tag="VGM24_03335"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEY4154424.1"
                     /translation="MDFKQIQELIKLVNKSNIGEVTVEEKGFKITVKQKEEHIQQVIA
                     APANAPSFQPAYAQPPASSAPPPSGSGKPKAPETASENLVTIKSPMIGTFYRSSAPDK
                     PSFVNVGDETEPGKVVCIIEAMKLFNEIESEVKGKIVKVLVEDASPVEFDQPLFLVDP
                     S"
     gene            3629..>4218
                     /locus_tag="VGM24_03340"
     CDS             3629..>4218
                     /locus_tag="VGM24_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY4154425.1"
                     /translation="MFKKILIANRGEIALRVIRTCREMGIKTIAVYSTADKDSLHVKF
                     ADEAVCIGKPASADSYLNIPHIMAAAEITNADGIHPGYGFLAENARFAEICGEHGIKF
                     IGPTPYMINSMGDKITAKETMIKAGVPVVPGGGGLLGSIDESKGLAYEIGYPVILKAT
                     AGGGGKGMRVVWQESELEHAFLTAKAEAGASFKNDG"
BASE COUNT         1102 a          942 c         1089 g         1085 t
ORIGIN      
        1 cacatgaata ccggtccact tgccgttttt atcaaacagg ttatatccgg aggcgttatc
       61 gtaacacagg tacatcccgg tccagattcc cggaacggta aaatagttat aaattaaccg
      121 cccggccttt acaaaatagc cgttccaggt tcccttgttg tcaaaggaga tcatcacatt
      181 ttgggcggca atggaaatat acccgaataa tttattggaa tcgttaaaga tgaacattcc
      241 gtcccaggtc ttgtaggagg gctgcaggct cttcatgaac accgggttca gcgtttcatt
      301 ggtctgcgga ttgagttgca ggttgatcat cgggttaatg gctgaattcc gggacggatt
      361 tacctcattg tctttctgcg gattcatatt ccagttaacg gaaggattga tggccaggtt
      421 actgtccgga tttatctgat tgttcagaaa gggattgata ttgggatctc ccttcgggtc
      481 atgcttgcgg aaaacagggt catcctgcgc ctgaagagtc gcaaaaggga tgaacaggca
      541 agccgtgaaa agcacaaatc ctcgcatacg gataacggtt gggttatagc gatacggaaa
      601 atgcgacttt tcgccggata ccaggatgtg aatggggaac agggcaaaag tactgcagga
      661 atctgagttt gtcaataggc cttttaaatg ccttttgccg gcaacagatc ctcagagcgt
      721 tctgagtaca tcattcattc ccctaacggc tgcggcgctc ttctcaaaca atgccttttc
      781 tccggcgttc aggtcgaaag aaacaatttt ttcccagccg tttttcccga tggtgaccgg
      841 cacgcccagg caaatatcct tttgcccgta ttcgccgttc agggcgacgc agcaggtaaa
      901 gagttttttc tcatcccgga caatgctttc caccagggcg gctccggcag cgccgggggc
      961 ataccaggca gaagtgccga tgagcttggt aagggttgcg ccacccacca tggtgtcggc
     1021 cacgatctgc ttttgctttt cctcgctcag cagggtcgtg acgggaatgc tgttccaggt
     1081 ggcaaagcgg atcagcggaa tcatggtggt atccccgtgg ccgccgatga ccagcgcgtt
     1141 cacgtcggcc ggactgcacc cgagatgctg gctgagctgg tacttaaagc gggaactgtc
     1201 cagggtgccg cccattccga taatgcggtt tttgggaaga ccgctggtct ggagggtaag
     1261 ataggtcatc gtatccatcg gattgctgat gatgatcaac acggcatccg gggaatattt
     1321 caggatattt tccgtaaccg tttttacaat tcccgcattg gttccgatca attcttcccg
     1381 ggtcataccg ggtttgcggg gcagcccgga agtgatgacc accacgttac ttccggctgt
     1441 ttttgagtaa tcattggtac tgccggtaat ccgggtatca aacccgagca gggtggccga
     1501 ctgcatcata tcctgcgctt ttccttcggc aaaaccttct tttatatcca gcaataccag
     1561 ttcttcagcg agttccttgc gtgcaatatt gtctgcacag gtggcaccta cggctcctgc
     1621 gccaactacg gtaactttca ttaaatatat ttttggtgag aagatgaata atacggttct
     1681 ccggcaggca aaggtaaagc ccgtgacgga aatttcaaga ggctgatcta tttatctccg
     1741 ggaactttcc cctgtggaag tttatttatc gtttgaactg aagccgttca ggatcgtttc
     1801 cactttctgg tttccttcaa aatgggtgat gtactggccc tttttatcgt acaaagccag
     1861 atagggcagg ttttgcattt tgtaataggg gggaagaaag tatttttcat cgcgtccgat
     1921 ccggatattg ggatggtctc ccagccggta gtaggaataa aaacttttca tttcatcaaa
     1981 tggctgaaaa gtggccatga cgatttcgat atctttgaat ttggaaaaat cggccagaat
     2041 atcactgatc tgatgtttgc aatgttcgca ttccgggctg aagaacatga gcaggatcct
     2101 ccggttcttt ttaatatcat cccgggttat atagtgaaca ctgtccactt tcagcaggcg
     2161 gataggtgga aaggcgggat ttcttacata ggcaggctct gcggaaacgg cggtttgggc
     2221 ctgaagattc agcagaaaaa acaacgagac aatcaggatg agcactcttt tcattctgga
     2281 tatttttatt aaaattaaag caaaatccgg gatgctgatc atttcatagc cggcgccgga
     2341 ttttttcccg tcccgctttc cggacggatc cggtggctgc agaaaattga aaaaagccta
     2401 aaaaccatta gttttgtcgc cctttagggt aaacaattaa ttatcattca tggcaaatac
     2461 agcagatatc agtcgcgggc tcattatcaa actcgacggc agtctttatt cagtgattga
     2521 ctttggtgaa aataaaaccg cccgggccgc tgcaaaggta tgggccaagt taaaaggggt
     2581 ggataacagc cgcagtattg aaaaaacctg gaacagcggg gataccatat atccggtgcg
     2641 ggtggaaaaa aaggattacc agtatcttta taaggatgat accggttata actttatgga
     2701 taccgaaacc tttgaacaga tcgctttgca ggaaaatctg gtggatgctc cgcagtttct
     2761 gaaagaaggg cagcaggtta ccgttttgat gaatacggaa acggacctcc cgatgggagc
     2821 cgaactgccg gataaaatcg ttttgctggt cacctattcg gagccgggtt taaaagggga
     2881 taccgctacc cgcaccctca aacccgctaa ggttgaaacg ggtgcaacgg tcaatgttcc
     2941 gctctttgta aacgaagggg aattgatccg ggtgaacaca aaaaccggtg aatatgtaga
     3001 aagggtgaaa gaataacact taccttagac ttccttatta atagtaaatg ccataactga
     3061 tggattttaa acagattcag gagttgatca agctggtcaa caaatcgaat atcggtgaag
     3121 taacggtcga ggaaaaggga tttaaaatta cagtaaaaca aaaagaagag catattcagc
     3181 aagtgattgc cgcgccggca aacgcaccgt cctttcagcc tgcctatgcc cagcctccgg
     3241 cctcctccgc gccgccgcca tcaggatccg gaaagcccaa agccccggaa acggcatctg
     3301 agaacctggt caccataaaa agtccgatga tcggaacttt ttaccgtagt tccgctccgg
     3361 ataaaccgtc ttttgtaaat gtgggtgatg aaaccgaacc cggcaaggtc gtttgtatca
     3421 tcgaagccat gaaacttttc aacgaaatag aaagtgaagt gaagggtaag atcgtgaaag
     3481 tgcttgtgga agatgcctct ccggtggaat ttgatcagcc tttgttcctg gtggatccga
     3541 gttaacctga aagccggata agtcgtttaa ttaaattgtt ttcaggcata tccggtggtt
     3601 gttttcaaat tttcaaattc tttaatcctt gttcaagaaa atactgatcg caaatcgtgg
     3661 agagatcgct ttacgggtca tccggacctg ccgcgaaatg ggaatcaaga ccatagcggt
     3721 ttattccacg gccgataagg atagtctcca tgtaaagttt gccgatgaag cggtttgtat
     3781 cggaaagccg gcaagtgctg attcatacct gaatatcccc catatcatgg ctgctgccga
     3841 aattaccaat gcggatggca ttcatccggg gtatggtttc ctggcagaaa atgcccgttt
     3901 tgcagaaatc tgtggcgaac atggcatcaa gtttatcggg ccgactcctt atatgatcaa
     3961 ttccatggga gacaaaatta cggccaagga aaccatgatc aaagccggcg taccggtggt
     4021 tcccggaggc ggcggtttac tcggaagtat cgatgaaagc aagggactgg cttatgaaat
     4081 tggctatccg gtaatcctga aagcaacggc gggtggggga ggaaagggga tgcgcgtggt
     4141 ctggcaagaa tcggagctgg agcatgcctt cctgacggcc aaggcagaag cgggggcctc
     4201 ttttaagaat gacgggat
//
LOCUS       DASYCU010000166         4269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4823350, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000166 DASYCU010000000
VERSION     DASYCU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4823350"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="VGM24_03345"
     CDS             <1..2085
                     /locus_tag="VGM24_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154426.1"
                     /translation="FDQGGLFKNTDIVPGYDNNINYARYNFRTNFDFDITDRLSAQVN
                     TSAEIEQNKGPYSSTGLSQSITTILAAVVEAPPNLSPGVVDGKLVSSVPGQSFYRGNP
                     LANMLGSGVFVYNNTYLNGSVRLNYKLDFITKGLSAHGTVSYRNMNQVATTHGKNVVT
                     YTIARLPDNTINYIPNSTDNPFNYAQTITSDRETYAEAGFDYKRSFGNHTVSALLLYN
                     QSKQVNPTLLFLVPHEYQGIVSRVTYNYKERYLAEVDLGYNGTENFAPGKRFGYFPAY
                     SAGWVASKESFFPKTSIISFLKFRASYGEVGNDVVGGTRFLYRPSSYTYTNADYHFGT
                     PGSSFNDYIGSLEGALGNPDLTWERAKKTDVGIDWWLFNDRVKITADWFREKRENILA
                     SFQTVPVVFGVALPQSNLGRMRNGGVDGEISFSDRIGNLNYWVKGTYTYAHNVIEYMD
                     EVHRPYPYEYRTGLRSGQIFGLVAEGFYNSWTEVNDPKRPVSTYNNNKIQPGDIKYKD
                     VNGDGKIDVDDQVPIGYSNFPEVIYGFSLGGSYGGFDFSLLIQGAAKVSFQYLQITQT
                     PFNSGSGVPNYWVDNSWTQDRYDNGADIKLPHLSISTDAQKHNYQPSTFWNVDGSYLR
                     LKNLEIGYRLPARYLKRVGVSATRIYLNGNNLLTMAHLLNGLDPEQRLANNSLYQYPL
                     TKTFNLGINLNF"
     gene            2195..3850
                     /locus_tag="VGM24_03350"
     CDS             2195..3850
                     /locus_tag="VGM24_03350"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154427.1"
                     /translation="MDKAPGVDVTEDTIFSSQAQVETLITGIYNLGVHNGYPFLDENY
                     RTNMDGIEAGCTDEGECTASWFWPNSTWNVGNINQQNGGSDFRWTDRWNVIRKCNIVL
                     ERIDGVPNTPQSYKDQVTGEVKFLRALAYFEMLKFYGGVPIVDKRFQLSDDLKVGRNS
                     VEDVVNFIVKNCDEAAALLPDVYPATLRGRATKGADLMLKSRTLLYAASPLFNTGTPY
                     LSMSDAGNNKFVCYGNYDVTRWQAAADAAKAVLDWAPHGGIALITDQGVDKNYKYMWE
                     RTDNNEIIFASQRNGLDGSAFGGMAPPVPPINALCGITVWLNFVQHYEKKDGTPQTWD
                     MNGGNDLNEKYDELDPRFKQTLSYNGAFWNTTIPNMQTYTGGQQSNLCFGGVWLKKAI
                     PDEWNNTSSQVSVNWTVFRLAEAYLNYAEALNEAQGPVPEAYDAVNTIRERSGMPDLP
                     TGLSQSDFRLRVQNERTVELAYEEHRFWDVRRWLIAENDGIMNGTMWGLTITPYPAPV
                     PDNTDFSYLPYAREVRVFSKREYLHPFPNGEMLKGYLVQNPGY"
     gene            3884..>4269
                     /locus_tag="VGM24_03355"
     CDS             3884..>4269
                     /locus_tag="VGM24_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4154428.1"
                     /translation="MASHINYIRNILPVSLILVCFSITHSAQAQELNGDSSAKRGQDV
                     LVNVAYGTQPKWLISGAVSTVKGSEMQQTFTSNLLNTLTGRLPGLTVRQSAGEPGLDA
                     PQLNIRGISTFGAGTTSIGPSSAPLIL"
BASE COUNT         1155 a         1004 c         1074 g         1036 t
ORIGIN      
        1 tttgatcagg gtggtctgtt taagaatacg gatatcgtac cgggctatga taataatatc
       61 aattacgccc ggtataattt ccggaccaat tttgattttg atatcacgga ccggttgtcg
      121 gcccaggtaa atacgtctgc ggaaatagag cagaacaaag gcccttacag ctctaccggt
      181 ttgtcgcaat ccataaccac cattctggcg gcagtcgtgg aagcaccacc caatttatca
      241 ccgggtgtgg tggatggtaa actggtgagt agtgtacccg gacagtcttt ttacagaggc
      301 aatccgctgg ccaacatgct tggctccggg gtgttcgttt acaacaatac ctatctgaac
      361 ggttcggtac ggctgaatta taaactggat tttataacaa aaggcttatc ggcccatggg
      421 accgtttcct accggaacat gaatcaggtg gctaccacgc acggaaagaa cgtggtaacg
      481 tataccatag caagactgcc ggacaacacg ataaattata ttcccaattc caccgacaat
      541 ccatttaatt acgcgcaaac cattaccagc gaccgggaaa cctatgcgga agccggattc
      601 gactataaga gaagctttgg caatcatacg gtctctgcgt tgctgttata taatcaaagc
      661 aaacaggtaa atccaaccct gctttttctc gttccccacg aataccaggg tattgtaagc
      721 agggtaacgt ataactataa ggaacgctac ctggcggaag tggatctggg atacaatgga
      781 actgaaaatt ttgcaccggg taaacgcttt gggtatttcc cggcctattc cgccggatgg
      841 gttgcctcaa aggaatcctt cttcccaaag accagtatta tctccttcct gaagtttaga
      901 gcgtcatacg gcgaagtggg aaatgacgtg gtgggcggca cgcgctttct ttacaggccc
      961 tccagttata cgtatacgaa tgccgattac cacttcggta cgccgggtag ttcctttaac
     1021 gactatatcg gttccctgga aggtgccctg ggaaatccgg accttacctg ggaaagggcg
     1081 aagaaaaccg atgtcggcat cgactggtgg cttttcaacg accgggtgaa aataacagca
     1141 gactggttca gggaaaaaag agagaatatc ctggcttcct tccagaccgt acccgtggtg
     1201 tttggagtcg cactgcccca atccaacctg ggcagaatgc gcaacggggg agtggatggc
     1261 gaaatttcct tcagtgaccg gatcggcaac ctgaactatt gggtgaaggg aacgtacacg
     1321 tatgcccata acgtaattga atatatggat gaggtccacc ggccttatcc ctatgagtac
     1381 cgcaccggcc tccggtccgg gcagattttc ggactcgttg ccgaaggatt ctataattcc
     1441 tggacagaag tgaatgatcc caaacggccg gtatccacgt ataacaacaa taagattcag
     1501 cccggcgata tcaagtacaa agatgtaaac ggcgacggaa aaatagatgt ggatgatcag
     1561 gtgcctatag ggtattcaaa ttttccggag gtcatttacg gcttttcttt gggcggaagt
     1621 tacggaggat ttgatttttc cctgttgatc cagggcgctg caaaggtctc atttcagtat
     1681 ttgcagatca cccagacgcc tttcaacagc ggatccggcg taccgaacta ttgggtagat
     1741 aacagctgga cccaggatcg ctacgataac ggggccgaca tcaagcttcc acacctgtcc
     1801 atatcaacag atgcccagaa gcataattac cagccatcca ctttctggaa tgtcgatgga
     1861 agctatctgc ggttaaagaa tcttgaaatc ggatacagac tgccggcgag atacctgaaa
     1921 agagtcggtg tgagcgccac gcgcatttat ctgaatggca ataacctgct taccatggcg
     1981 catttgctga acggtttgga tccggaacag cggctggcaa acaattccct ctatcagtat
     2041 ccgctgacga agacctttaa tctggggatc aatttaaatt tttaaaaaaa taaaatattt
     2101 aacagtgaaa aatataataa ttgcaatagg agcatgctgc accgtttttg cagtttccgt
     2161 agtttctttt tcctgtaaaa aaatcaataa ttttttggat aaggcccccg gagtcgacgt
     2221 aacagaagat accatttttt cttcccaggc acaggtggaa accctgatca cgggaattta
     2281 caacctcggg gttcacaatg gctatccctt cctggacgag aattaccgca ccaatatgga
     2341 tggtatagaa gccgggtgta cggatgaagg ggaatgtaca gcctcctggt tctggcccaa
     2401 cagcacctgg aatgtgggaa atataaacca gcaaaacgga ggctctgatt tccggtggac
     2461 ggatcgttgg aatgtgatca ggaagtgcaa cattgtgctg gagaggatcg acggggtgcc
     2521 gaatactcct caaagctata aggatcaggt gacgggggaa gtaaaattcc tgcgcgcgct
     2581 ggcttatttt gaaatgctga aattttatgg tggggtgccc atcgtcgaca aacgctttca
     2641 gctttccgat gacctgaaag ttggccggaa cagtgttgaa gacgtggtga attttatcgt
     2701 gaagaattgt gatgaggcgg ctgcccttct gccggatgta tatcctgcca cgttgagagg
     2761 acgggcgacc aagggcgctg acctgatgct gaaatcccga acactgcttt atgcagccag
     2821 cccgctcttc aataccggaa ctccttatct gagcatgtct gatgcgggca acaataagtt
     2881 tgtctgttat ggaaattatg atgtaacccg atggcaggcg gcggcggatg cagccaaggc
     2941 cgttctggac tgggctcctc atggcggcat tgcgctgatc acggatcagg gagtagataa
     3001 gaactataaa tacatgtggg aaagaacaga taataacgaa attatttttg cttcccagcg
     3061 caatggtctc gacggttccg cttttggcgg aatggctcct cctgttccgc caatcaatgc
     3121 cctttgcgga ataaccgtgt ggctcaattt cgtgcaacat tatgaaaaga aggacggcac
     3181 tccgcaaacc tgggatatga acggggggaa tgatctgaat gagaaatatg acgaactgga
     3241 tccccgcttc aaacagaccc tgtcctataa tggcgcattc tggaatacca ccattcccaa
     3301 tatgcaaacc tatacgggtg gacagcaatc caatttatgt tttggaggcg tctggctgaa
     3361 gaaggcaata cccgatgaat ggaacaatac cagctcacag gtcagtgtca actggaccgt
     3421 cttccgcctg gccgaagcct atttaaacta tgcggaggcg ctgaacgaag cacagggacc
     3481 tgtaccggaa gcatatgatg ccgttaatac catccgggaa cgatccggaa tgcctgacct
     3541 tccgacaggc ctcagccaga gcgatttccg gttgcgggtg caaaatgaaa ggacggtcga
     3601 actggcttac gaagaacacc ggttctggga tgtgagacgc tggttgattg cagagaatga
     3661 cggcatcatg aatgggacga tgtggggact gaccatcact ccttatcccg cacccgttcc
     3721 ggacaatacc gacttcagct atttgcccta cgcacgcgaa gtgcgggttt tttccaagcg
     3781 cgaatattta catccatttc cgaatgggga aatgttaaaa ggctatttgg ttcaaaaccc
     3841 tggttattga tttttttaat tcatcattta agaataaaat catatggcta gccacataaa
     3901 ctatatacga aacattctcc cggtttccct aatcctggtc tgtttcagta ttactcatag
     3961 cgctcaggct caggagctga acggagacag ctcggccaaa agaggccagg acgtcctggt
     4021 taatgtcgcc tacggcacgc aaccgaaatg gttgatttcg ggtgcagtct ccaccgtaaa
     4081 gggatcggaa atgcaacaaa ctttcacttc caatctgctg aatacgctca ccggaagatt
     4141 acccggtctt accgtaagac aaagtgcggg agaacccgga ttggatgcac cccagctcaa
     4201 tatacgcggt atcagcacct ttggagcagg tacaacttcc atcggaccga gttctgctcc
     4261 gttgatcct
//
LOCUS       DASYCU010000167         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2214704, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000167 DASYCU010000000
VERSION     DASYCU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214704"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VGM24_03360"
     CDS             complement(<1..649)
                     /locus_tag="VGM24_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY4154429.1"
                     /translation="MTETRSMKEITQLAWNKRPLIISGPCSAETEEQVMETAVGLQKT
                     GKVDVMRAGIWKPRTRPGSFEGIGTKGLPWLQRAKQVTGIPVAVEVATAKQAEDALHF
                     EVDILWLGARTTVNPFSVQEVADALRNVDVPVLIKNPINPDLELWIGAVERVAKAGIK
                     QIGLIHRGFSSYGNSEYRNAPMWHLAIEMKRRFPDLLFINDPSHICGRRDILLDVA"
     gene            complement(731..2389)
                     /locus_tag="VGM24_03365"
     CDS             complement(731..2389)
                     /locus_tag="VGM24_03365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4154430.1"
                     /translation="MSTFEGLGLNEQLLQAVAALGFTQPTPIQEKAIPVLLSGTKDLV
                     GLAQTGTGKTAAFGLPLLQLLNIQERFPQALVVCPTRELCLQIVSEMELFKKFTPGVQ
                     VLAVYGGSSIGMQIRDLRRGVQIVVATPGRLIDLIERKAINLEEIKYVVLDEADEMLN
                     MGFQDDIEFILKNTPKREATWLFSATMPAEIRRVSRKYMKEPLEITVGKTNTANINVD
                     HQYFVTNAHHRYEALKRLIDFNPGIYGIVFTRTKADAQNIAEKLTREGYEIDALHGDL
                     TQQQRDKVMGEFRDKTLQMLIATDVAARGIDVAGITHVINYELPDDVEVYTHRSGRTG
                     RAGNTGICLSIIHSREVAKLRQIERIVQVPFRKVEVPSGKDVCRKQFFHFMEKLLQAD
                     ISHGDYETYVPMLAEKFADISKEEVLKRVAAMEFDRFLKYYENAEDLNVRAPSREAPV
                     RGHRERIPIGSGMPRKTFSSGNGDFVKLFVNLGTKDGFYKASFLQFVLDMSDLKKEVL
                     GKIDMKEMNSWLEIDRRSAPQMIRAIDGKNYRGRRIRMNDANSR"
     gene            complement(2491..>2973)
                     /locus_tag="VGM24_03370"
     CDS             complement(2491..>2973)
                     /locus_tag="VGM24_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY4154431.1"
                     /translation="TEYSGPEAAVHDAFRGRSCVICEKGKSAADAVETMEKIYRLLGM
                     NLVYMEARSHDVHAAYVSHISHITSFALANTVLEKEREEDAIFELAGGGFESTVRLAK
                     SSPSMWAPIFMQNRENVLDVLNEHISQLRKFKSCLEKENYGYLRELMENANKIRRILG
                     "
BASE COUNT          645 a          845 c          695 g          788 t
ORIGIN      
        1 gagccacatc cagcaaaata tcacggcgac cgcagatatg ggaaggatca ttgataaata
       61 acaggtccgg aaaccggcgt ttcatttcaa tggcgaggtg ccacatgggc gcattccggt
      121 attccgaatt tccataagaa ctgaacccgc ggtgaatgag tccgatctgt ttgatacctg
      181 ccttggccac ccgttccacc gcaccgatcc agagttccag gtcgggattg atcgggtttt
      241 tgatcagcac gggcacatcc acgttccgga gagcatcagc aacttcctga acactgaacg
      301 ggttcacggt cgtgcgcgcc cccagccaca aaatgtccac ttcaaaatga agggcgtctt
      361 ccgcctgttt ggctgtggcc acttccacgg ccacgggtat accggtcacc tgttttgccc
      421 tttgcaacca ggggaggccc ttggtcccga tgccttcaaa acttccgggc cgggtgcggg
      481 gcttccagat accggcccgc atcacatcca cctttccggt cttttgcagc cccaccgcgg
      541 tttccattac ctgttcttcg gtttccgcgc tgcaggggcc ggaaataatc aggggacgtt
      601 tgttccacgc gagttgggta atttctttca ttgatcgcgt ctcagtcatg ggaagtcaat
      661 ttattatttt gaaaatgtta tttatttgaa aagagaaaaa tttagcagca aggagccggt
      721 ctttcgaaaa ctacctgctg ttcgcgtcat tcatcctgat ccttcttccc cggtaattct
      781 ttccgtcgat agcccggatc atttgcggag ccgatctccg gtcgatctcc agccagctgt
      841 tcatttcctt catatctatt tttcccaaca cttctttttt gaggtcactc atatccagca
      901 caaactgcag gaaactggcc ttataaaagc catctttggt acccaggttc acaaagagct
      961 ttacgaaatc cccgttcccg gaagaaaatg ttttacgagg cattccgctc ccgatgggga
     1021 tccgttcccg atggccgcgg accggcgctt cccggctcgg tgcgcgcaca ttcaggtcct
     1081 ccgcattttc ataatatttc agaaaacggt cgaattccat tgcggctacc cgtttgagca
     1141 cttcttcctt gctgatatcg gcgaattttt ccgccagcat gggaacatag gtctcatagt
     1201 cgccatgact gatatccgcc tgcaataatt tctccataaa atgaaagaac tgcttgcggc
     1261 aaacatcttt tccggacgga acttccacct tccggaaagg aacctggaca atgcgttcga
     1321 tctgacgcag tttggcgacc tcccgggaat gaatgatgga aaggcaaata ccggtattcc
     1381 ccgcccggcc ggtccttccg ctgcggtgcg tatacacctc cacatcatcc ggcaattcat
     1441 aattgatcac atgggtgatc ccggcaacat ctattccccg ggcggccacg tcggttgcaa
     1501 tcagcatttg cagggtcttg tcccggaatt cccccattac cttgtcccgc tgctgttgcg
     1561 taaggtctcc atgcagcgca tcgatctcat atccctcgcg ggtcaatttt tcagctatat
     1621 tttgagcatc cgcttttgtc cgggtaaaaa cgatcccgta aattcccggg ttaaaatcga
     1681 tcaggcgttt caacgcttca taccggtgat gcgcattggt aacaaaatac tggtggtcga
     1741 cattaatatt ggccgtattg gttttaccta cggtgatctc caggggttct ttcatgtatt
     1801 tgcggctgac ccgcctgatt tccgccggca tggtggcgct gaataaccac gtcgcctctc
     1861 ttttcggcgt gttcttcagt atgaattcaa tatcatcctg gaatcccata ttcagcattt
     1921 catccgcctc atccaggacc acgtatttga tctcttcgag gttgatggcc ttgcgttcga
     1981 tcaggtcgat cagccggccg ggggttgcca ctacaatctg tacgccccgg cgaagatccc
     2041 ggatctgcat gccgatggac gatcctccgt aaacggcgag cacctgaacg cccggtgtga
     2101 attttttaaa tagttccatt tcactcacaa tctgaaggca aagttcgcgg gtcgggcaaa
     2161 ccaccagggc ctgcggaaaa cgctcctgga tattcaggag ctgaagcagt ggcagcccga
     2221 aagcggctgt ttttccggtt ccggtctggg ccaggccaac gaggtccttg gtcccgctca
     2281 ataaaacagg aatagccttc tcctgtatgg gggtgggttg tgtaaagcca agcgccgcaa
     2341 cggcttgcag cagctgttca tttaacccta acccttcaaa tgtgctcata cggttcaaaa
     2401 attttgcgca aaggtactgt tttctgagcg gagaccccct ttttggggcg gacagctcat
     2461 tggaaatcac tgaccggctt tacgataact tcatcccaat atccgcctga tcttattcgc
     2521 attctccatc agttcccgca ggtacccgta attttctttt tccaggcaac tcttgaattt
     2581 tctcaactgg ctgatatgct cattcagcac atccagcaca ttctcccggt tctgcataaa
     2641 tatgggcgcc cacatggacg gactgctttt tgccaaccgc acggtgcttt caaaacctcc
     2701 gcccgccagt tcaaaaatgg catcctcctc ccgctctttt tccaatacgg tgttcgccag
     2761 ggcgaaagag gtaatatgtg atatatggct cacataggcc gcatgcacgt catggctccg
     2821 ggcctccata taaaccaggt tcattccgag taaccggtag atcttttcca tggtctctac
     2881 ggcatctgcc gctgattttc ctttttcgca gattacacag gagcgtccgc ggaaagcatc
     2941 gtgcaccgca gcttccggcc cgctgtattc cgt
//
LOCUS       DASYCU010000168         4727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1347282, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000168 DASYCU010000000
VERSION     DASYCU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347282"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /gene="upp"
                     /locus_tag="VGM24_03375"
     CDS             <1..625
                     /gene="upp"
                     /locus_tag="VGM24_03375"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY4154432.1"
                     /translation="SILCDWVAELRDENIQRDRMRFRRNLERIGEIAAYEISRHLLFE
                     EREVQTPLGISKSMLLQAQPVLATILRAGIPIHQGLLNIFDKADNAFIAAYRKHDRDG
                     SFEISLNYISCPDLENRILILSDPMLATGSSMVKTIQYLRAEGHPAQIHIVTAIACTV
                     GLEYVRREEPSCKIWCAAIDDELTAKGYIVPGLGDAGDLAFGDKVQI"
     gene            813..1850
                     /locus_tag="VGM24_03380"
     CDS             813..1850
                     /locus_tag="VGM24_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HEY4154433.1"
                     /translation="MRRIILALIYVSFLLPGPAEAQDPAFSQFFASPLSLNPALTGKF
                     NGVLRVAGNYRNQWPQINNAFITSTVSIDGNMLSGRLPAGDAWGVGVLAMTDKTANGI
                     LNSNYFSVSTAYQKSLDENGWNQLGIGFQGTYANKRLDGTKLQFEDELDQQGGWTIPS
                     SEVIDGHQVNLSYFDLNVGVLYNGSTDGNNNFYFGASAYHITQPKESFTGNAYYVLHP
                     RITLHGGYSFPLSPENNNNFIHFSGVFSKQANATNTTLGAAWSSNLVGNDENPLNFYL
                     GVWARFSNLTDAIIPYVGLDFGPFTLGMSYDVNVSSLKSASQSQGGIEVSLIYIKKHS
                     DGTRSIPCPKF"
     gene            complement(1847..2464)
                     /locus_tag="VGM24_03385"
     CDS             complement(1847..2464)
                     /locus_tag="VGM24_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY4154434.1"
                     /translation="MTGRAKKLDQSFYDRTDVLKISRELLGKILISNFDGMRTSGRIV
                     ETEAYAGVGDRASHAFGGKRTARMEAVYGSPGTIYMYICYGMHHLFNVVTNKKEIPHV
                     ILIRALEPLEGIPVMLQRTGKKFADHSLTRGPGNVSRALGLNKDHSGTNLLSGEIYIA
                     DDGLKYGKEQVVITKRIGVESAREDADLLYRFIVKGNPYVSARKS"
     gene            2520..3281
                     /locus_tag="VGM24_03390"
     CDS             2520..3281
                     /locus_tag="VGM24_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEY4154435.1"
                     /translation="MLKEIVIAIQAYRKAHLFIGKHNLWKWILIPGFIYMILFSIGIY
                     FFWISADSAVSHLSQLIGIDRWLHRQHSAALSFLFMMGGIMVRLTMIFFYFSLFKYLF
                     LIIGSPIFAYLSEKTESIIEGKEFEFRYPQFFKDIRRGIRLAFRNCLWQTVYTVSILI
                     LSFIPLAGWITPVISSLVECYYYGFSMLDYSCERHQLSPANSIVYISKRRGLAIGNGL
                     VFYLMHLVPFAGWVLAPSYALIAATISLYHQNIEE"
     gene            3278..3988
                     /locus_tag="VGM24_03395"
     CDS             3278..3988
                     /locus_tag="VGM24_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY4154436.1"
                     /translation="MKSSATVYLIPTFLDEETLQVLPGYITTAVKACEVFFVENERSA
                     RRFLKKLWKEMEIDRYSWFVIHKAGEPVKQEFLRQLEQGKQIGILSEAGCPGIADPGQ
                     LLVAAAHQAGARVKPLVGPSSILLALMASGMNGQQFQFNGYLPVDAQERIKRIKELEA
                     LSQKQSSTQIFIETPYRNQVLLDAILKSCKAETRLCVAVNLTGKEESIQTRSIGEWRK
                     IKPVPGKQPSIFLLDAGG"
     gene            complement(3985..4386)
                     /locus_tag="VGM24_03400"
     CDS             complement(3985..4386)
                     /locus_tag="VGM24_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154437.1"
                     /translation="MKKFLTLFILVVLVGFGTFIYWRYYRVFGDGVNAGQLNNLMRKG
                     YIFKTYEGRLIQAGFRGNGPGSVQSYEFRFSVVDKKVADQLMANSGKEFELHYKEYMA
                     ALPWRGTSVFIVDSIISMKDSRALLQNPYSP"
     gene            complement(4398..>4727)
                     /locus_tag="VGM24_03405"
     CDS             complement(4398..>4727)
                     /locus_tag="VGM24_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HEY4154438.1"
                     /translation="ALESDAGGFTPRAFSVKANPEQVSRLNSWIPLLNPYGVTEILNM
                     GAGADVEPLNESLGVPAAELMPDSQRYFDYHHSAADVFEIVNKRELELGAINMAALVY
                     LVDKYGF"
BASE COUNT         1133 a         1255 c         1126 g         1213 t
ORIGIN      
        1 ttccattctc tgcgattggg tggcggagtt gcgggacgag aacattcagc gcgaccggat
       61 gcggttccgg cgaaacctgg aacggatcgg cgaaattgcc gcttacgaaa tcagccgcca
      121 tcttctattt gaagaaaggg aggtacagac gccactgggc atatccaaat ccatgctgct
      181 ccaggcccag ccggtgctgg ctaccatcct gcgagccgga attcccattc accaggggct
      241 gctgaatatc ttcgacaaag ccgacaacgc ctttattgcc gcataccgta aacacgaccg
      301 ggacgggtct tttgagatca gcctgaacta tatttcgtgc ccggacctgg agaaccggat
      361 cctgatcctc tcggacccga tgctggccac cggatcttcc atggtcaaaa ccatacaata
      421 tctccgggcg gaaggccatc cggcacaaat ccatatcgtt actgccattg cctgcacggt
      481 gggcctcgaa tacgtccggc gggaagaacc atcctgtaaa atctggtgtg cagccattga
      541 tgatgaactc accgccaagg gttatatcgt tcccggcctg ggggacgccg gggacctggc
      601 ttttggggat aaggtccaaa tctagcttct ccaggcttcc ggatattcgc ccggcacttt
      661 cgcgtatggg gttttttacc tattttcagg ttccagttcc tttttcctat gaatcctgtt
      721 tccgttttcc aaaaagaaaa ccggcctttc gcaacaacct gcttttccgg ttcaataaaa
      781 tgacttatcc atcaggaaaa ggcctgtact atatgagaag gattatctta gccttaattt
      841 atgtttcatt tctgctaccc gggccggcgg aggcgcagga tcctgcattc tcccagtttt
      901 tcgcctcccc gctttcactg aatccggcac ttaccggtaa attcaacggg gtattgcggg
      961 tggcgggtaa ctaccgcaat caatggcccc agatcaacaa cgcgttcatt acctcgacgg
     1021 tttcgattga cgggaatatg ctgtcgggcc ggcttcctgc aggagatgcc tggggcgttg
     1081 gcgttttggc catgaccgat aaaaccgcga acggtattct caacagcaat tatttttcgg
     1141 tgtccaccgc atatcagaaa tcactcgacg aaaatggctg gaaccagctg ggtatcggtt
     1201 tccagggaac ctatgcgaat aaaaggctgg acggcaccaa actgcaattc gaggatgagc
     1261 tggaccagca gggcggatgg accattccca gctcggaggt cattgacggt caccaggtga
     1321 acctgagcta ttttgatctg aatgtgggtg tcctctataa cggatcaaca gacggcaata
     1381 acaattttta ttttggcgca tcggcatacc atatcacgca gccaaaggaa tcctttaccg
     1441 ggaacgccta ttatgtgctg catccgcgca tcaccctgca cggcggttat tcatttccgt
     1501 tatctccgga gaacaataac aatttcattc atttcagcgg agtattcagc aaacaggcca
     1561 atgcgaccaa taccaccctg ggagcagcct ggtcgagcaa cctcgttgga aatgacgaga
     1621 accccctcaa tttttatctg ggtgtgtggg cgcggttcag caatctgacg gacgccatta
     1681 ttccttacgt tggtctggat tttggcccgt ttaccctggg catgtcgtat gacgtgaatg
     1741 tttcttccct taaatccgca tcgcaaagcc aggggggtat tgaagtctcc ctcatctata
     1801 tcaaaaaaca ttcggatgga acgcggtcca taccctgtcc gaaattttaa gactttcttg
     1861 cactcacata aggattccct ttcacgataa accggtaaag gagatcggca tcctctctgg
     1921 ccgattcaac tccgatcctt ttcgtgatga caacctgttc tttcccgtat ttcagtccat
     1981 cgtcggcaat atatatttca ccggaaagca gattggttcc cgaatgatcc ttgttcaacc
     2041 ctaacgcgcg cgaaacattt ccgggccccc gggtaaggga atgatcggcg aacttttttc
     2101 cggtcctttg cagcatgacc ggaattcctt ccagtggttc cagggcccgg atcaggatca
     2161 catgggggat ttctttctta ttggtcacca cgttaaacag gtgatgcatt ccgtaacaga
     2221 tatacatata gatggtgccg ggcgatccgt acacggcttc catccgggcc gtccgctttc
     2281 ctccgaaagc atggctggcc cggtctccca cgccggcgta tgcttccgtt tccacaatgc
     2341 ggcccgaggt gcgcattcca tcgaaatttg aaatcagtat tttcccaagc agttcccgcg
     2401 aaattttcaa tacatcggtt ctgtcataaa aggattgatc gagttttttt gcccttccgg
     2461 tcatatacat atcttaaagc gtaaattagt gctatattca acatctttaa aaaaatgggt
     2521 tgctaaaaga aattgtcatt gccatacaag cctatcggaa ggcccacctg ttcatcggca
     2581 aacataatct atggaagtgg atcctgattc cgggattcat ttatatgatc ctgttcagca
     2641 taggcatcta ttttttctgg atctcagcgg acagtgccgt ttcccatctc agccagctca
     2701 tcggcattga ccgctggctt caccgccagc acagtgctgc cctgagtttt ttgttcatga
     2761 tgggcggaat catggtgcgg ctcacgatga tcttttttta tttttctctt tttaaatatt
     2821 tattcctgat catcggctcg ccgatttttg cctacctgag cgaaaaaacc gagtccatta
     2881 ttgaaggaaa agaattcgaa ttccggtatc cgcaattttt caaagacatc cggcggggga
     2941 tcaggctggc attccggaac tgtctctggc aaaccgttta taccgtatcc atattaatcc
     3001 tatcctttat tccgctcgcc ggctggatca ctcccgtaat ttcctccctc gtggaatgct
     3061 attattacgg attctccatg ctggattaca gttgcgaacg gcaccagctt tctcccgcga
     3121 acagtattgt gtatatcagc aaacggcgcg gcctggccat tggcaacggg ctggtttttt
     3181 atctgatgca cctggttccc tttgccggat gggtgctggc cccttcttat gccctgattg
     3241 ccgcaacgat cagtctctac caccagaata ttgaggaatg aaatcgtctg ccaccgtcta
     3301 tctgatacca acctttctgg atgaggaaac gctccaggta ttgcccgggt acatcacgac
     3361 ggctgtaaag gcctgcgagg tcttttttgt ggagaatgaa cgcagtgccc gccggttcct
     3421 gaaaaaattg tggaaagaaa tggagatcga ccggtattcc tggtttgtca ttcataaagc
     3481 cggtgaaccg gttaaacagg aatttctccg tcagctggaa caaggaaaac agatcggcat
     3541 actgagcgaa gccggatgcc cgggcatcgc cgatccgggc cagttgctgg tggcagcagc
     3601 tcaccaggcg ggagcccggg taaaaccgct ggtcggaccc agttccattc tccttgccct
     3661 catggcaagt gggatgaacg gccagcagtt tcaattcaac ggctaccttc cggttgatgc
     3721 gcaggagcgg atcaaaagga tcaaagagct ggaagcgctg tcccaaaaac agtcctccac
     3781 ccagatcttt atcgaaaccc cttaccggaa tcaggtgctg ctggacgcga tattaaaaag
     3841 ctgcaaggca gaaacgcgct tatgcgtcgc cgttaacctg accggaaaag aggaatccat
     3901 tcagacaaga tccatcggag aatggcgcaa aatcaaaccg gttcccggaa agcagccttc
     3961 catctttctg cttgatgcgg gaggctaagg actgtacggg ttctgcagta atgccctgga
     4021 atctttcatg gaaataatac tatccacgat gaacaccgag gtcccgcgcc agggcaaagc
     4081 ggccatatat tccttatagt gcagttcaaa ttccttaccg ctattggcca ttaactggtc
     4141 tgccactttt ttatctacca cggaaaaacg gaattcgtac gactgcaccg aaccgggtcc
     4201 gtttccccgg aatcccgcct gaatcaaccg gccttcatac gtcttaaaaa tatagccctt
     4261 tctcatcagg ttgttcaatt gaccggcatt gacgccatcg ccgaaaaccc ggtaataccg
     4321 ccagtaaata aacgttccga atcccaccag aacgacgaga atgaagaggg ttagaaattt
     4381 tttcatttcg gtaagtttta gaatccgtat ttgtccacca ggtaaacgag tgcggccata
     4441 ttaatggcgc ccagttccag ttctcttttg tttacgatct caaatacgtc agcggctgaa
     4501 tgatgataat caaaatagcg ttgggaatcc ggcatcagtt ctgccgcagg cactcccagg
     4561 ctttcattca gcggttctac gtccgccccg gcacccatgt ttaaaatttc cgtgacaccg
     4621 taaggattca atagggggat ccaggagttc aggcgggaaa cctgctccgg atttgcctta
     4681 acactgaatg ccctcggcgt aaaaccaccg gcatcgcttt ccagggc
//
LOCUS       DASYCU010000169        10397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2795700, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000169 DASYCU010000000
VERSION     DASYCU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10397
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795700"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1119
                     /locus_tag="VGM24_03410"
     CDS             235..1119
                     /locus_tag="VGM24_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4154439.1"
                     /translation="MYYVVYFIAWLFSLLPLWILYRLSDLIAMLLFYIVPYRKKLVLA
                     NLATAFPDRTCAERKKIARHFYRNFVDHFLETLKLMSAGKNFLNEHFIIDNPEILEPY
                     RREGRRCQLQLGHSFNWEYANNGFALLSGQPLIVIYMPIGNRIFNRLLNHIRSKTGAI
                     QIPATDARKQMLAFRHQPYILTLVADQAPGSPEMAYWLNFFGRPTPFVRSPERSARSG
                     NTPAIFVHFYKSSRGKYRAKLFSGSEAPGSLTEGELTRRYVEFLQWSIREQPALYLWS
                     HNRWKYPWKPEYQSRLID"
     gene            complement(1116..2522)
                     /locus_tag="VGM24_03415"
     CDS             complement(1116..2522)
                     /locus_tag="VGM24_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4154440.1"
                     /translation="MYIQQLYTGCLSEAAYYIESQGEAAIIDPLRDIEEYLLLAKERK
                     ATIKYIFETHFHADFVSGHLDLAQASGAPIIYGPETETAFPIYKAKDGEVFRLGDLTL
                     EVLHTPGHTVESSCYLLKDAHGKAYAIFTGDTLFVGDVGRPDLSSGDLDKEELAGMLF
                     DSLQKKIKPLPDEVIIYPAHGQGSSCGKNLGPDTYSTLGEERKTNYALKQEDRQEFIR
                     SVTEGLNVAPQYFPINARINKEGYESLEGVMESALTPLSLADFKGLILQEDTIILDTR
                     FSEVFTEGFIPGSISIGLDGRFAEWAGSLLPFDKPLVLIAEPGKEKESAVRLARVGFD
                     QMKGFLEGGFKTWKSAGEKIDLIINVEADELALDLRFDPKLRVVDVRRETEYGDGHLA
                     SAQNIPLETLTDPGTMADFNDTDNIYLHCGSGFRSVIACSLFKRQGIHNLRNVVGGYE
                     AIRALSGIETVKENSVLN"
     gene            complement(2605..2955)
                     /locus_tag="VGM24_03420"
     CDS             complement(2605..2955)
                     /locus_tag="VGM24_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4154441.1"
                     /translation="MNGTISNYWMVVNAHGGDDASVKVDFINLKKAAMILRALNHKLR
                     QQIVRVLDEHKKLTVTELYIHLRLEQSVASQHLAILRRAGIVKTERDGKFIHYTINPG
                     RIADIMKTVQLLIV"
     gene            complement(3036..3704)
                     /gene="tsaB"
                     /locus_tag="VGM24_03425"
     CDS             complement(3036..3704)
                     /gene="tsaB"
                     /locus_tag="VGM24_03425"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY4154442.1"
                     /translation="MLLLINTSAEKAFIALSQAGKLLCMEENPLPKEHATWLHEAVRR
                     MLERAQVGMSALQAVAVVAGPGSYTGLRVGMAAAKGFCYALKIPLICLNSLQLMGEAM
                     IPLAKEKKALICPMIDARRQEVFTAVYSVEMQEILAPRALILDKTSFDHILHTDRVIF
                     SGSGSEKWKLISESRNALFAPQIDEKESFAIISQRYFAAERWAGPDSSDPIYLKEFYT
                     HAKN"
     gene            complement(3792..4124)
                     /locus_tag="VGM24_03430"
     CDS             complement(3792..4124)
                     /locus_tag="VGM24_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4154443.1"
                     /translation="MLEAAADEDSMEKARKIFGSRIRFVKDVPGMVSPRILSMIISEA
                     YFTLGAGTSSRDEIDTAMKLGTGYPLGPFEWAKKIGIHRVAALLRLLGEEDPLYEPAP
                     LMKLEMEG"
     gene            complement(4623..5651)
                     /gene="pheS"
                     /locus_tag="VGM24_03435"
     CDS             complement(4623..5651)
                     /gene="pheS"
                     /locus_tag="VGM24_03435"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY4154444.1"
                     /translation="MEELLEKLETYRREIKESQLGNPDAAESYRIRFLGSKGIVKNLF
                     QEMKGVPVEKKKEFGQILNEFKQLAELKYEEARNSAAGDSGAPNGTDWSLPGDPVPVG
                     TRHPISLVRNRIVQIFGRLGFSVAEGPEIEDDWHNFTSLNMPADHPARDMQDTFYINR
                     NPDWLLRTHTSNVQSRVMEKQKPPIRIICPGRVYRNETISARAHCFFTQTEGLYIDEQ
                     VSFADLKQTLYFFVQEMFGKSVKIRFRPSYFPFTEPSAEMDISCLICGGSGCSVCKHT
                     GWVEILGSGMVHPNLLQNFHIDPNKYTGFAFGMGVERICQLKYRINDLRLYSQNDLRF
                     LGQFVSAV"
     gene            5704..6840
                     /locus_tag="VGM24_03440"
     CDS             5704..6840
                     /locus_tag="VGM24_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEY4154445.1"
                     /translation="MLYSLHILDYLDPVNLREVSNDEDYNDGQVGKLISVYEEEFPDI
                     MQADLILVGCREERGARRSRNNINGPDCIRQQLYRLYHWHTDLKLADVGNIKTGANLA
                     DSYAALKTVVSELGSLGKTVIILGGSHDLSLAQYQSYLNRKKLIEVTCVDALVDIHID
                     SPLKSDNFLMEMLTDEPNYIHHYNHIAFQSYYVHPNMLETMDKLRFDCYRVGKVKEHI
                     DEMEPVLRNTNMLSFDIAAIAHHYAPANHLSPNGLSGEEACALMRFAGLSPNISSIGI
                     YGYIPEKDREDLTAKQIAQMLWYTIDGRSKGRREATLNDKEYFNEYHTAFAEVETTFL
                     QSKKTNRWWMQLPDQKYIACSYNDYLLASSNEIPERWLRAQERL"
     gene            6901..8304
                     /locus_tag="VGM24_03445"
     CDS             6901..8304
                     /locus_tag="VGM24_03445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY4154446.1"
                     /translation="MNIESCPPKGPRPVTRHPLSAFIPAILILTWLLVPGLNFAQGRK
                     NDERALKDLLESPSLRTAHIGVLVYDDSLKKDIAAFQDDKYFVPASNTKLFSLYAGLK
                     YLGDSLVGIRYETNDTAVFVFPSGDPSLLHPDFQQQPVIDFLKNTDKKIYIVDSAWQE
                     EAQGRGWGWDDYNDDYAVERSLLPVYGNFIRWTQEKDVLKGHPSFETTPRIFSSPEVS
                     WKVKFSPDSLPKKFFVKRTIDSNLFEIRTGDEVHAEQDVPFVTNKLAAAMDLLRDTLG
                     KPVYTLHKPPVFNTPASIIYSRPADSVYIPMMHRSDNFFAEQTLLMVSNQKLGVMNDH
                     RIIDSLLKTDYADLPQTPSWADGSGLSRFNLFSPRDFVTLLEKFKNEFGMDRMKKILP
                     TGGGPGTLKNYYLNDSGSIFAKTGSLSGVICLSGYLYTRKNRLLEFSVLVNNHYSSGS
                     AVRKAVEAYIEYIRKNY"
     gene            complement(8347..10248)
                     /gene="mutL"
                     /locus_tag="VGM24_03450"
     CDS             complement(8347..10248)
                     /gene="mutL"
                     /locus_tag="VGM24_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY4154447.1"
                     /translation="MTNGKIMDDLIQLLPDNIANQIAAGEVIQRPASAVKELMENAID
                     AGASQVKLIINDAGKSLVQVVDNGRGMSVTDARMAFERHATSKIRNIDDLFRIRTMGF
                     RGEALASIAAVAQVDLKTKRATDETGTGIEIENSLVKRQEPVAWPNGTSIAMKNLFFN
                     VPARRNFLKSNAVETKHIVDEFVRVALSFPDIQFLLFSNGQEMFHLEEGTLKQRIVQI
                     LGAHYNSKLVSVQENTDYLNISGFVGKPDTAKRTRGDQYFFVNNRFIRSAYLNHAVMT
                     AYQQMIPGDSFPLYILFIEIDPSRVDINVHPTKQEIKFEDEKLIYAFVQAAVRHSLAQ
                     FSITPSLDFDLDPSIQGLDAVNKPMTAETKTALESSSLFRTFSQKNQAHFIEPSSKSD
                     LAHWTERGSADPGFLSGNAPFRAAQSPQPLASGGQPLQSLYSRYGADPLFRDQDPIRL
                     LQLQNTYIVCETDGGFLLIHQQSAHERVLYEQFINALTEKPLATQPGLFPVTISLATP
                     DSILLQEMLTDLRSLGYAIEPFGKDTFIIQGTPAGLEAGSEKKIIEKMLEQCKHSGSE
                     KIFPLREKMIRSLAWQQSVKPGTGLSETEMRSLAESLFRCAQPNMTPNGKPVFAEYKK
                     EYLEKIFGR"
BASE COUNT         2459 a         2721 c         2565 g         2652 t
ORIGIN      
        1 gccgctgtat ggcctgctga ctttcggcct cgcccatttg tcgttcacct atttcgagcg
       61 tccgttgctc tcactcaagt ataaattttt cccgtattcc gttaaagcag aataatattt
      121 atttaccggc cggctgcggc caattttttc tgtgacacgc atcatcgtat ttccctctac
      181 gttattttgc cgatatcaat aaatcgacgg ttctttgcag cataggatgt gtgcatgtac
      241 tacgtcgtct attttatcgc ctggcttttt tccctccttc cgttatggat tctctaccgc
      301 ctgtcggacc ttatcgcaat gcttcttttc tatattgtcc cctatcgcaa aaaactcgtc
      361 ctcgccaatc tggccacggc ctttccggac agaacctgcg ccgaaaggaa aaaaattgca
      421 aggcattttt accggaattt tgtcgaccat tttctggaga cgctcaaatt aatgtctgcc
      481 ggcaaaaatt ttctgaatga acatttcatc atcgacaatc cggaaatcct cgaaccctac
      541 cggcgggagg gacgccgctg ccagctccag ctcgggcatt cctttaactg ggaatacgca
      601 aacaatggct tcgccctgct gtctggccag ccgctaatcg taatctatat gccgatcggc
      661 aacaggattt ttaaccgcct cctgaatcat atccggtcaa agacgggagc aatacagatt
      721 ccggccaccg acgcacggaa acagatgctg gccttccgtc accagcccta tatcctgacg
      781 ctcgtggcag accaggcccc gggttcgccg gaaatggcgt actggctgaa tttttttggc
      841 cggccgacgc cttttgtcag gtcgcctgaa cgcagcgccc ggtcgggaaa tacgcccgcg
      901 atctttgtgc atttttacaa atcatcccgg ggaaaatatc gcgcaaaact attttccggc
      961 agcgaagcgc cgggcagtct gacggaggga gaactaacgc gtcgttatgt tgagttcctg
     1021 caatggagta tccgtgaaca gccggctttg tatctgtgga gtcacaaccg gtggaaatat
     1081 ccctggaagc cggaatatca atcccggctt atcgactagt tcagcacgct gttttccttt
     1141 accgtttcga tgccactgag ggcccggatg gcttcatagc cgccaacaac atttctcaga
     1201 ttgtgaatgc cctggcgttt gaacagggag caggcgataa cgctccggaa tccgcttccg
     1261 caatggaggt aaatattatc ggtgtcgttg aaatcggcca tggttcccgg atccgtgagc
     1321 gtctccagcg gaatattctg agccgaagcc agatgaccgt ccccatactc cgtttccctt
     1381 cgcacatcaa ccacgcgaag tttcggatcg aaccggaggt cgagtgccag ctcatccgct
     1441 tccacattga tgatcagatc gattttctca ccggcggatt tccaggtttt gaagccgcct
     1501 tccaggaatc ccttcatctg gtcaaacccg acccgggcca ggcggactgc actttctttt
     1561 tcttttccag gttcggctat cagcaccagc ggtttatcaa acggcaggag actgcccgcc
     1621 cattcggcaa accgcccgtc cagcccgatg ctgatggaac cggggataaa tccttctgta
     1681 aaaacttcgg aaaaacgcgt gtccagtatg atcgtgtctt cctgcagaat gaggcccttg
     1741 aaatcggcca ggctcagcgg tgtcagcgcg ctttccataa cgccttcgag gctttcataa
     1801 ccttccttgt tgatccgggc gtttatcgga aaatattgcg gagccacgtt caatccttcc
     1861 gtgaccgacc ggataaattc ctgacggtcc tcctgcttaa gggcatagtt tgtctttcgc
     1921 tcttcgccca aggtactata ggtatcgggc cccagattct taccgcagct gcttccctgg
     1981 ccgtgagcgg gataaatgat cacctcatcg ggcaggggct ttattttttt ctggagggag
     2041 tcaaacagca ttccggccag ttcctcttta tccaggtcgc cgctgcttag atcgggtctg
     2101 cccacatctc ccacaaacag cgtgtcgccc gtaaaaatcg cataagcctt tccgtgagca
     2161 tctttcagca gataacagga cgactcgacc gtatgaccgg gagtatggag tacttccagc
     2221 gtaagatcgc ccagtctgaa gacctcaccg tcctttgcct tataaatggg aaaggcggtc
     2281 tccgtctcgg ggccgtaaat gatgggtgct cccgaagcct gcgccagatc caggtgaccg
     2341 ctcacaaaat ctgcatgaaa atgggtctcg aaaatatatt tgatggtggc tttccgctct
     2401 tttgccagca ggaggtattc ctcaatatcc cgcagcgggt cgatgatggc agcctctccc
     2461 tggctctcaa tataataagc cgcttcactc aggcatccgg tatagagttg ttgaatatac
     2521 atgggggatg attgtgtgca aagctactga attcggcggg gtcagggtcc ggaccagggt
     2581 accctccgcc cccgaaggca gggcttacac aattaacaac tgcacggttt tcataatatc
     2641 cgcgattctt cccgggttaa tggtatagtg aatgaattta ccgtcacgct cggttttaac
     2701 gatgccggcc cggcgaagga ttgccagatg ctgggaagcg acggattgct ccagccgcag
     2761 atgaatataa agttcggtta cggtgagctt tttgtgctca tccagcacac gcacaatctg
     2821 ctggcggagc ttatgattta aagcccgcag gatcatcgct gcctttttga gattgataaa
     2881 gtcgactttc acagatgcat cgtccccccc atgggcgttg acgaccatcc aataattact
     2941 gattgtacca ttcatgacgg taaacacatt gaaaaggtct gtaaattaat tatgaatgct
     3001 accaaataga gaacccacaa gattttgtaa tatagctaat ttttcgcatg agtgtagaat
     3061 tctttcagat aaatgggatc ggaactatcc ggtccggccc atctttccgc tgcgaaatag
     3121 cgttgagaaa ttattgcaaa tgattctttt tcatcaattt gtggggcaaa gagcgcattc
     3181 cttgattcag atatgagctt ccatttttct gatccgctac cggaaaaaat cacccgatcg
     3241 gtgtgaagaa tatggtcaaa tgatgtttta tctaatatca gggcccgtgg cgccaggatc
     3301 tcctgcattt ccaccgaata aacagcagtg aatacttcct ggcggcgggc atcgatcatc
     3361 ggacagatca gggccttttt ttcctttgcc agcggaatca tggcttcccc catcagctgc
     3421 aagctgttga ggcagataag gggaattttc aaagcataac aaaatccctt cgctgcggcc
     3481 attccaaccc gcaacccggt gtaggaaccc ggcccggcca caacggctac cgcctgaagt
     3541 gcgctcatcc cgacctgcgc cctttccagc attcgccgga ccgcctcatg aagccaggtc
     3601 gcatgttctt tcggcaatgg gttttcttcc atacacaata gctttcctgc ctgggaaagg
     3661 gcgatgaatg ctttttcggc tgaagtgttg atgaggagga gcaaattggc tgatccctgg
     3721 ttttgttggt ggatccgggc gcaagttaat aagtaaaaaa tattatatcc gggataggct
     3781 gacagcgggc attatccttc catctccaac ttcatcagag gggcaggctc atagagggga
     3841 tcctcctcgc ccagcaaacg caaaagagcc gcaacccggt ggatcccgat ttttttcgcc
     3901 cactcgaaag gacccagtgg gtaaccggtg cccagcttca tggcggtatc tatttcatcc
     3961 cgcgagctgg tgcctgcgcc cagggtaaaa taggcttcgc ttatgatcat ggaaagaatg
     4021 cggggactta ccattccggg aacgtctttt acaaaccgga tccggctgcc aaatattttc
     4081 cgggcttttt ccattgaatc ttcatccgcc gctgcttcca gcaagggtga ttctaaaaat
     4141 cccggccatc cgttgatccg gataaaaccg ggctgaattc cggaaagcgg atgtattaca
     4201 gaatttacaa atacgggtgc tggaaagaga cgggataggg tttccgccct ttcccgcatt
     4261 ggcggattga tttctttgga aactgtcgca ggatttttgg cggctctttt tccgggaccc
     4321 ggcttccacg tatttttaat ttcaccctcc ccttcctttt gaactgcttc cggatggcct
     4381 tgtccgaacg cattccccgg ttccgtttca aacaacaggt ccaggtatgc atctgcgttc
     4441 tgacttatca gatcctgaag atcttttgca aaaacgatcc ggtgctcccg gaagacggga
     4501 aatgtttcaa gtccggcttt ttgcgttgcg gaggcaagaa cggacagtgt tgatggctgg
     4561 tgttcgatgg cagaaaggat ttgtggtcga ggcttctggc cggtatagtt tatatcaggc
     4621 ggtcaaaccg ccgaaacaaa ctggcccaga aatctcagat cattttgtga ataaagccga
     4681 aggtcattga tccggtattt cagctggcag atcctttcca cgcccattcc gaaggcaaac
     4741 ccggtatatt tattcggatc aatatggaaa ttctgcagca ggttcggatg gaccattccg
     4801 cttcccagga tttccaccca gccggtatgt ttgcatacac tacatccgct tcctccgcag
     4861 atcaggcaac tgatgtccat ttcagcgctg ggttccgtga aaggaaagta ggagggtcgg
     4921 aaacggattt tcaccgattt accaaacatc tcctgtacaa aaaaataaag ggtttgcttg
     4981 aggtcggcaa aggatacctg ctcatcaata tacaatcctt ccgtctgggt gaaaaaacaa
     5041 tgcgcccggg cgctgatggt ttcattccgg tacacccggc ccggacaaat gatgcggatc
     5101 ggcggttttt gtttttccat tacccgcgac tgcacattgc tggtatgcgt gcgcaggagc
     5161 cagtccggat tcctgttaat ataaaaggta tcctgcatat cccgcgccgg atgatccgcc
     5221 ggcatattga gcgaagtgaa attatgccag tcgtcttcga tctcggggcc ttccgccacg
     5281 gaaaaaccaa gtcttccgaa gatctgcacg atgcggttcc tgaccaggct gatgggatgc
     5341 cgggtaccta ccggaaccgg atcgcccggg agtgaccagt ccgttccgtt tggggcaccg
     5401 gaatctccgg cagctgaatt tctggcctct tcatatttta attccgcgag ctgtttgaat
     5461 tcgttgagga tctgtccgaa ttcctttttc ttttccaccg gtaccccttt catttcctga
     5521 aaaagatttt ttacaattcc cttggaccct aaaaaacgga tgcggtaaga ttcggcagcg
     5581 tcgggatttc ccaattggct ttcctttatt tctctccggt aggtctccag tttttccagt
     5641 aattcttcca tgctgcaaag ataaaaggaa aaacatgtgt aagtttgttt caaatcgtgg
     5701 cgaatgctgt acagtctgca tatcctggat tatctcgacc cggtaaatct ccgggaggtt
     5761 tccaatgatg aagattacaa tgacgggcag gtggggaaac tgatcagtgt ttatgaagag
     5821 gagttccccg acatcatgca ggcagacctg atcctggtcg gatgccggga agagcgcgga
     5881 gcccggcgtt ccaggaacaa tatcaacggc cccgactgca tccggcagca gctgtaccgc
     5941 ctctatcact ggcacacgga tctgaagctg gcggacgtgg gaaatatcaa gaccggcgcc
     6001 aacctggcgg atagttatgc cgccctgaaa acggtcgtca gcgagctggg cagcctggga
     6061 aaaacagtga tcattttggg aggatcgcac gacctgagcc tggcccagta ccagagttat
     6121 ttgaaccgga aaaaactgat tgaagtgacc tgtgtggacg cgctggtgga tatccatatt
     6181 gattcgcccc tcaaaagcga taatttcctg atggaaatgc tgaccgacga acccaattac
     6241 attcaccatt acaatcacat tgccttccag agttattatg tgcacccgaa tatgctggag
     6301 acgatggaca agctgcggtt cgattgttac cgcgtaggca aagtgaagga gcacatcgat
     6361 gagatggaac ccgtgctgcg gaataccaat atgcttagtt tcgacatcgc cgcgatcgcc
     6421 catcactatg cgccggcaaa tcatttatcc ccaaacgggc tttccggaga ggaagcctgc
     6481 gcgctgatgc gctttgccgg actgagcccg aatatcagca gcatcggcat ttacggatat
     6541 attccggaaa aagaccggga ggatctcacg gcgaaacaaa tcgcgcagat gctctggtat
     6601 accatcgatg ggcgaagcaa aggccgtcgc gaagccaccc tgaacgacaa ggagtatttc
     6661 aacgaatacc atacggcttt tgcggaagtg gagaccacct tcctgcaaag taaaaagacc
     6721 aatcgctggt ggatgcagct gcccgatcag aaatacatcg cctgttccta taacgattat
     6781 ctgctggcca gcagcaatga gatccccgaa aggtggcttc gggcacagga acggctctga
     6841 aggacctgta tttttcgttt tctcatgata cgatcagatc atttaaacct acagataaaa
     6901 atgaatattg aatcctgccc tccaaagggt ccccggcctg tcacgcgcca tccgctctcc
     6961 gcttttattc cggcaatcct tattctgaca tggctgctgg tcccgggcct taactttgcc
     7021 cagggcagga aaaatgacga gcgggcgctt aaggacctgc tggagagccc gtctctccgg
     7081 actgcacata tcggcgtatt agtgtatgat gattctctaa aaaaagatat tgccgctttc
     7141 caggatgata aatattttgt gccggcgagc aataccaagt tattttcctt atacgccgga
     7201 ttgaaatacc tcggggattc gctggtgggt atccgttatg agaccaatga taccgcggtc
     7261 tttgtttttc cgagtgggga tccgagcctg ctgcatccgg atttccagca acagcccgtt
     7321 attgatttcc tgaaaaatac ggataaaaag atctatatcg tggacagcgc ctggcaggaa
     7381 gaagcacagg gccggggttg gggctgggac gattacaatg atgattatgc cgttgaacgc
     7441 agtctgctgc cggtttacgg aaattttatc cgctggaccc aggaaaaaga cgtgctaaaa
     7501 ggccatccgt cctttgaaac cactccccgg atattttctt cgccggaggt cagctggaaa
     7561 gtaaaattca gcccggatag cctgccaaag aaattttttg ttaaaagaac gatcgattcc
     7621 aatctattcg agatccggac cggcgacgag gttcatgccg agcaggatgt cccctttgtt
     7681 acgaataagc tggcggctgc gatggacctg cttcgggata ccctgggaaa gccggtttac
     7741 accctgcata agccaccggt tttcaacact ccggcttcca ttatttattc caggccggcc
     7801 gattccgttt acattccgat gatgcaccga agtgacaatt tttttgcaga acaaaccctg
     7861 ctgatggtaa gcaatcagaa actgggagta atgaatgatc accggatcat cgacagcctg
     7921 ttgaaaaccg actatgcgga tctcccccag acaccgtcct gggccgatgg cagcggcctc
     7981 agccgtttca atttattcag tccccgggac ttcgtcaccc tgctcgaaaa atttaagaat
     8041 gaattcggga tggaccggat gaaaaagata ttacccaccg gcggcggccc cggaacactt
     8101 aaaaactatt acctgaacga cagcggatcc attttcgcaa aaaccggttc cctgagcggc
     8161 gtgatctgcc tgagcggata tctctatacg cgcaaaaacc ggctgctgga attttccgtc
     8221 ctggtcaaca accattacag cagcggctcc gccgtacgca aagccgtgga agcatatatc
     8281 gaatatatcc ggaaaaatta ttgagcagcg ggcggaccgg aagatcatgg cccgggttat
     8341 tttccatcat cttccaaaga tcttctccag atactccttt ttgtattctg caaaaacggg
     8401 ttttccattc ggcgtcatat tcggctgggc gcaacgaaaa aggctttccg ccagggatcg
     8461 catctcggtt tcggaaaggc cggttccggg tttcacggat tgctgccagg ccagggagcg
     8521 gatcatcttt tcgcggagcg gaaatatttt ttcactaccg gaatgcttgc actgttcgag
     8581 cattttttca atgatctttt tttcgctgcc ggcctccaga ccggccggcg ttccctgaat
     8641 gatgaaggta tcttttccga agggttcgat cgcatatccc agagaccgca ggtccgtcag
     8701 catttcctgc agcagtatgc tgtctggtgt tgccagggaa atggtaaccg gaaacaaccc
     8761 aggctgggtg gccagcggtt tttccgtcag cgcgttgata aactgttcgt acaacacccg
     8821 ctcgtgcgcg ctctgctggt ggatcaataa aaacccgcca tccgtttcgc agacaatata
     8881 ggtgttctgc agttgcagga gccggatcgg gtcctggtcc cggaacaggg gatccgcgcc
     8941 gtaacgggaa tagagcgact gaaggggttg accgccggaa gctaagggtt gcggggactg
     9001 tgccgcgcgg aaaggcgcat ttcccgaaag aaaaccggga tccgcgctgc cgcgttctgt
     9061 ccaatgtgcc agatcgcttt ttgaagaagg ctctataaaa tgcgcctgat ttttttgcga
     9121 gaaggttcta aaaaggctcg aggattccag cgccgttttg gtttctgccg tcatcggttt
     9181 atttacggca tccagaccct ggatcgaggg atccagatcg aaatccaggc tgggcgttat
     9241 gctgaattgg gccagggaat gcctcacggc tgcctgtaca aaagcataaa tcagtttttc
     9301 atcttcaaat ttaatctcct gtttggtggg atgcacattt atgtccaccc gcgacggatc
     9361 gatctctatg aataaaatgt atagcggaaa actgtctccc ggaatcattt gctgataagc
     9421 cgtcataacg gcatgattca ggtaagcact ccggataaaa cggttattca caaaaaaata
     9481 ctggtctccg cgggtgcgct tagcggtatc gggcttgccg acaaagccgc tgatatttag
     9541 ataatccgtg ttttcctgaa ccgagaccag cttcgaatta taatgggcac ccaggatctg
     9601 tacaatacgc tgcttaagcg ttccctcttc gaggtgaaac atttcctgtc cgttgctgaa
     9661 caggaggaat tgaatatccg gaaacgaaag cgcgacccgg acaaactcat ccacgatatg
     9721 cttcgtttcc accgcattgc ttttaagaaa attgcgcctt gccggtacat tgaagaaaag
     9781 atttttcatg gcgatactgg tgccgttcgg ccaggccacc ggttcctgcc ttttcaccag
     9841 gctgttttca atttcaatgc cggtacccgt ttcatccgtt gccctctttg ttttcagatc
     9901 cacctgggcc accgccgcga tggaggcaag cgcttctccg cggaagccca tggtgcggat
     9961 ccggaaaaga tcatcgatat tcctgatctt ggacgtagcg tgccgttcaa acgccatgcg
    10021 cgcatcggtc acactcatgc cccggccatt gtccacgacc tgcacgaggg actttcccgc
    10081 atcatttata attaatttta cctggctggc ccccgcatca atggcgtttt ccatcagttc
    10141 tttcacggcg cttgccggcc gctggatcac ttcaccggcg gcaatctggt tcgcaatatt
    10201 atccggaagc aactgaatca ggtcatccat aatttttccg ttggtcatct gaaacgggca
    10261 atttaatgga aataaatttc attttccttc gtaggtttac cgggagtttc cctctgatcg
    10321 ttaaaaatgt ccgccaatga aaaaaatcct ttgcgttttt ttggtcgtcc tttccctttc
    10381 tctccaagcc caaaatc
//
LOCUS       DASYCU010000170         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3666293, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000170 DASYCU010000000
VERSION     DASYCU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3666293"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGM24_03455"
     CDS             <1..691
                     /locus_tag="VGM24_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY4154448.1"
                     /translation="PRTGEIPSEELQEKADRMKKRLENLGEVNPTAVEAFQEMKRRYD
                     FIVEQKNDLVSAKDTLLQTIQEVEATANQQFLDTFGKVRENFQKVFKALFTDEDTADL
                     ILENAEDLAETGIDIVAKPKGKRPSSITQLSGGEKTLTATALLFAIYLIKPAPFCILD
                     EVDAPLDDANVGKFTNMIRQFSDNSQFIIVTHNKMTMSSVDVIYGVTMQEAGVSKLVP
                     VDFRSLSTQAS"
     gene            complement(734..1735)
                     /locus_tag="VGM24_03460"
     CDS             complement(734..1735)
                     /locus_tag="VGM24_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY4154449.1"
                     /translation="MIKQCLTGCLLLAFALSASAQKIKMLSLANPVSLRGLCPVNDRV
                     IWVSGSSGTVGLSTDAGINWKWITVPHYEKADFRDVEAFSDQEALIMGDTEPAIILKT
                     KNGGKTWVTVFADSSRPLFLDAMDFSGDQGILLGDPAGNRIFLAQTKDRGDSWKIIPK
                     KNLPTAEKGEAFFAASGTNIKWLSAGRYAFVSGGKKSALYLDSSRKFQLPLSQGKEST
                     GANSIAVDPADPNQAFICGGDFSEDRAATGNAVAIRFHPFHQQIPRTAPHGYRSCVEY
                     INGRTLICCGTSGVDISTDGGMNWKLISGNSFHVCRKAKSGSRIFFAGSRGAIAVLE"
     gene            complement(1784..2110)
                     /locus_tag="VGM24_03465"
     CDS             complement(1784..2110)
                     /locus_tag="VGM24_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154450.1"
                     /translation="MTTLSQVIEKLRLKRQDNEFRLDPEGLSIGNGKFYQPQDLKIIK
                     TYRFEGESDPADSSILYLLEGNDGTIGYSVDSYGAYSNFDDMQYDDFIRKVPVEERDE
                     QQIFPD"
     gene            complement(2180..>3089)
                     /locus_tag="VGM24_03470"
     CDS             complement(2180..>3089)
                     /locus_tag="VGM24_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY4154451.1"
                     /translation="VILYGESGTGKEVIAQTIHNKSLRRDKPFIAMDCGTLSKELAGS
                     ELFGHMKGSFTGALSDKEGHFELANGGTLFLDEVANLSYEIQAALLRVIQERKFKRVG
                     GNKEMDVDVRIIVASNEDLQSAYRNGRFREDLFHRFNEFSIVLPPLRNRKEDIPLFAQ
                     FFLDNANKELNKKVEGFEEEVSRMFLNYPWPGNLREFRNVVRRSALLSPSGVIGTRVL
                     PAEIAGVQWDNIPREASSSGHTQESINREMDLKNAAAQAEYETIMNVLKKVNFNKSKA
                     AEILKIDRKTLYNKIKLFESGMGRPR"
BASE COUNT          729 a          802 c          794 g          764 t
ORIGIN      
        1 acccagaacc ggagagattc cgtctgaaga actgcaggaa aaagcggacc ggatgaaaaa
       61 gcgcctggag aatctcggag aagtgaaccc cacggccgtt gaagcctttc aggaaatgaa
      121 aaggcgctac gattttattg tcgagcagaa gaatgacctg gtcagcgcaa aagatacttt
      181 attgcagacc atccaggaag tggaagccac ggcgaaccag cagttcctgg acaccttcgg
      241 aaaagtgaga gagaattttc agaaggtttt caaggccctg ttcaccgatg aagatacggc
      301 ggacctcatc ctggagaatg cagaagacct ggccgagacc ggcatcgata ttgtggccaa
      361 gccaaaaggg aaaaggcctt cttcgataac gcagttgagc gggggagaaa aaacgcttac
      421 cgccaccgcg ctcctgtttg cgatttatct catcaagccg gcgcctttct gtatcctgga
      481 tgaagtggac gccccgctgg atgacgcaaa cgtgggcaag ttcaccaata tgatccgcca
      541 gttcagtgat aattcccagt ttattattgt gacccacaac aaaatgacga tgagcagcgt
      601 ggatgtcatc tacggagtga ccatgcagga agccggcgtc agcaagctgg ttcccgttga
      661 tttcagatcg ctgagcacgc aggcaagctg aatagtccca tagaagaatt tggatgaagg
      721 gccgtttttc tacttactcc aatactgcaa tggcccccct gctgccggca aagaaaatgc
      781 ggctcccgga ttttgctttg cggcatacat gaaaactatt tccggagatc agtttccagt
      841 tcattccccc gtccgtggaa atgtccactc cggaggttcc gcaacagatc agcgtgcggc
      901 catttatata ttccacgcaa ctccggtaac catgcggcgc cgtccggggt atctgctgat
      961 gaaagggatg aaagcggatg gcgacggcgt ttccggtggc ggcacggtct tcggaaaaat
     1021 cgccgccgca gataaaagcc tggttagggt ctgccggatc gacggcaatg gaattggcgc
     1081 cggtgctttc cttaccctgg gaaagcggca gttgaaattt acgggaggaa tccaggtaca
     1141 gggcggattt ttttcctccg gatacgaatg cataccggcc ggctgacaac catttgatat
     1201 tggttccgct ggcagcaaaa aaagcttcgc ctttttcggc agttggtaaa ttctttttcg
     1261 gaatgatctt ccaggaatcg ccccggtctt ttgtttgggc aaggaagatc cggttgccgg
     1321 ccggatcgcc cagcaggatt ccctggtcac cggaaaaatc catcgcatcc aggaacaggg
     1381 gcctgctgct gtccgcaaat acggtcaccc aggtctttcc cccgttcttt gtttttagaa
     1441 ttattgccgg ttccgtatcc cccatgatca gcgcttcctg atcgctgaag gcctccacat
     1501 cgcggaagtc cgctttttca taatgcggaa cggttatcca tttccagtta atgcccgcat
     1561 cggttgaaag gccgaccgtt cccgatgaac cgctcaccca gatcacgcgg tcattcaccg
     1621 ggcataatcc gcgcagggaa acgggattag ccaggcttag catttttatt ttctgtgccg
     1681 aagcgctcag ggcaaaagcc aggaggaggc agccggtcag gcattgcttt atcatgcggc
     1741 gaatgccccg gttagaggaa ggttatatga aggtgttgcg gaactaatcg ggaaagattt
     1801 gctgttcatc tctttcttcc accggaacct tgcgaatgaa atcatcgtat tgcatatcat
     1861 cgaagttgct gtaggccccg taagagtcca cgctatagcc gatcgttccg tcatttccct
     1921 caagtaaata taatatggaa gaatctgccg gatcggattc cccttcaaac cggtaggtct
     1981 tgatgatttt aaggtcctgg ggctggtaga acttgccgtt gccgatgctg agtccttccg
     2041 gatccaggcg gaactcatta tcctggcgtt tcagccgtag cttttcgatc acctgactga
     2101 gcgttgtcat ttcagctggc ttaatcatat cctgaggttt tgttaatgaa caaaaagacc
     2161 caaatgccgt accagttatt taccggggac ggcccattcc ggattcaaaa agtttgatct
     2221 tgttatagag ggtcttccgg tctattttca ggatctcggc cgcttttgat ttattgaagt
     2281 tcactttctt gagcacattc ataatggttt catattccgc ctgggcagcc gcatttttaa
     2341 gatccatttc ccggttaatg gattcctggg tatgtccgga agaactggct tccctgggaa
     2401 tattgtccca ctgcacacct gcaatttcgg ccggaaggac ccgggtccca atgacacccg
     2461 aaggagataa aagcgcagac cgcctcacca cgttccggaa ttcacggagg tttcccggcc
     2521 agggatagtt aaggaacatc cgggacactt cttcttcaaa gccctccact tttttattga
     2581 gctctttgtt ggcattatcg aggaagaact gggcaaagag gggaatgtcc tcctttcggt
     2641 tccttaacgg aggtaagaca atggaaaatt cattgaaccg gtggaaaaga tcctcccgga
     2701 atcgcccgtt ccggtaagca ctctgcaaat cctcgttgga agccacgatg atccggacat
     2761 ccacatccat ttccttattg ccgccgacgc gcttgaattt tctttcctgg atcacccgga
     2821 gcagggccgc ctggatttcg taggaaagat tggccacctc atccaggaac aatgttccgc
     2881 cattggcaag ctcgaaatgc ccttccttgt cgctcagcgc tccggtgaac gatcctttca
     2941 tatgcccgaa gagctcactt ccggcgagct ccttggacag ggttccgcag tccatggcga
     3001 tgaacggctt gtctctccgc aggctcttgt tgtgaatggt ttgtgcgatc acttccttac
     3061 ccgttccgct ctctccgtaa aggatcacg
//
LOCUS       DASYCU010000171         9603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_329524, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000171 DASYCU010000000
VERSION     DASYCU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329524"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGM24_03475"
     CDS             complement(<1..398)
                     /locus_tag="VGM24_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154452.1"
                     /translation="MKKYLVITIDVEPDCSPTWHYADPLTFKGVSEGIAQILQPLFNK
                     QGISPTYLVNNVVLEDESSVSCFLHLEGKFELGTHLHPEFILPDKKYENYAGKKGVAN
                     CCFYPPDIESEKIRNITLLFEKALGYKPTA"
     gene            complement(395..2299)
                     /gene="asnB"
                     /locus_tag="VGM24_03480"
     CDS             complement(395..2299)
                     /gene="asnB"
                     /locus_tag="VGM24_03480"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY4154453.1"
                     /translation="MCGFAGILNNAFPVRRQQLAGIAAAVCFRGPDDCGLRLYDQHLH
                     PVVDGNNALFFNRLAIIDLDPRSNQPFEDERYSLLFNGEIYNYRELKKTLQKEGVLFH
                     TSSDTEVLFHVLVKWGSKGLNRLNGMFAFCWIDRKEKKFIIARDRTGIKPVYYKQQGK
                     TFIFGSELDSVLRLSDGLPPIRPESVRQFLWMQFIPSPNTIVQGIFKLPPGHYLEGNW
                     ESLDRHQAPAAEPYWDAYSKIASGAPDADPEQLENILVDSLSRQMEADVPLGLFLSSG
                     VDSSLLAALVNKHFAGTQAFNFFTVAFNEITASDESDDAIAFIRGFHNPGLRSHKLSI
                     DAKLVGEKLELLYDYFDEPFGDTASLLNWVISAKARESVTVALSGDGADELFWGYPRY
                     DQWKNPSLHLFNKLNIPGNLASLARPFLPGKYWRAKYALELETDPVRRHFSLFLSPAL
                     GHLLQHPVWTDDFWALEHTVLIRDREDLAAVLDIKTYLADAMLYKVDRASMAASLEVR
                     VPYLDNTVIDYALALPFIYKSNPQFKHKAILKQLLQKLAPHYPIDRPKKGFNFPLDKW
                     LRHAWKDKVLSSLNKESVASLGLDDKRYLPMVEKYYGGDKKFTIVVWYLLNLALWNQK
                     FKKISPLRPI"
     gene            complement(2310..2897)
                     /locus_tag="VGM24_03485"
     CDS             complement(2310..2897)
                     /locus_tag="VGM24_03485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4154454.1"
                     /translation="MEKLVWKLKVWGGKLQRIILRYIFKFDKWHVFTLTEKQYAKDII
                     AYCNSRPVRNRFAEIGCGLGDIVRHVYYRERQGYDLDEKALKAARFLNSIMGGGKIRF
                     SGFTFPGSPLPGNYDVIVLVNWIHHIEPAVLRSSIADYFNVALNEGGVIIIDTVQDPE
                     YEYNHDVRFLTDGIGAGVSKLGDYERERQIWLIKK"
     gene            complement(2900..3712)
                     /locus_tag="VGM24_03490"
     CDS             complement(2900..3712)
                     /locus_tag="VGM24_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4154455.1"
                     /translation="MEELKTAEKLSSQAYWDLVLEEAKLPRINSRRSYHYRVTMDFID
                     PFLRNKPYTSFFEVGCGSSGWLPYFGQEYGLTVSGIDYSATGCRLAEENLRILGIPYG
                     EIICRDIFENNCTDGKKYDVVFSYGVIEHFENPEDIIRIFSSFLNPGGLMITLVPNLN
                     GSMGWLSRLFIPDIYKMHRVISRQQLQRYHESNALHNLKTNYAGTFTLAVLPLIRSTH
                     WLLREGSTQRKIALSGFSVFDKLASRTFRLLKLNIPTRRFSPYIISIAAKNI"
     gene            complement(3690..5237)
                     /locus_tag="VGM24_03495"
     CDS             complement(3690..5237)
                     /locus_tag="VGM24_03495"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY4154456.1"
                     /translation="MLKVESYRKGIVLSTVFNILNKGLVFCNSLIIAYFFGAQLKMDI
                     YFYAYNTIVILAAFITSLNSSVLIPESMRLRAQESERRGMFFLNFFLYLYAAITIGVC
                     LLFLINPVSALHAVSGFSRESLEQQARILYLAVPLIFLMPVTNLLTDIMTSYKFFSIP
                     MIAGIINGIFSILFIVLFHRLLDVSSLLAGLLLSYTLNFILLVSLMKKRLNWTFGFHR
                     LKIEKRIWKNIAFAQAGNITSSLSAYAPLYLLSGFNGGIITSLNFAQQISSLPTTLIT
                     NQFSAVAGIKFNESYAGKDFGKLNSTFLSTANFLMFILFPVAGIFFLFPSQIVSILLE
                     HGAFGKQGVAYTSLFLQYLGLLLPMLVINTLFARLFMASHKIMESFWYQVCFNIVLIA
                     TLYLSIHRFGFAAYPITLVCVHVLNILGCYFLEKKYFGTIQYAQVLKNMLMLLVVNGV
                     VGAGIYLFISKAAFQSGFLSLAVGASLYLSLIFVIGFKSNINDTFNDFVREFWRKGLN
                     YGRTKNR"
     gene            5520..7346
                     /locus_tag="VGM24_03500"
     CDS             5520..7346
                     /locus_tag="VGM24_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4154457.1"
                     /translation="MKILLTYLKPYKMLVALTLFLASVNIGFSLFDPIIFGKIVNLSN
                     EYVQARKLHQDFSADHFFTAFSWTHPGVINLLLLSIGVAMVSRLAKNFQDYFMNVVVQ
                     KFGAKVFTDGLQHAMKLPYQDFEDQRSGETLGVLQKVREDTERFINYFMNVLFGVIVG
                     VIFVFTYAAIWINVSIPIVYLAGILLLTFISNVLSRKIKSIQKNIVNQTTSLAGSTTE
                     SLRNIELVKSLGLTQEEVTRLNKNTYKILGLELTKVKRVRSISFIQGTFVNTLRQVIL
                     FILMWLIFKDKMNAGQLVTMQIFSFFVFGPLQDIGNIIMSYREAEASLNNFDDLMKKV
                     PEPQSANPKHLGKIEQLSFREVIFKHKSVQHYAIDRISFDVRVGETIAFVGPSGAGKS
                     TLMKLLVGLYRPMGGRILYNHLDENSIDFDDLRNQIGFVTQDTQLFSGTIRENLLFVN
                     PKATQEDLLDVLQKASCNGLLQRAEHGLETRIGEGGLKLSGGEKQRISIARALLRRPR
                     VLIFDEATSSLDSLTEEEITSTIRKVSSQKNQITILIAHRLSTIMHADRIYVLEKGEV
                     VETGTHKALLDEKGLYYAMWRQQIGERKNSQDIPETEMSVIR"
     gene            7353..8111
                     /locus_tag="VGM24_03505"
     CDS             7353..8111
                     /locus_tag="VGM24_03505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4154458.1"
                     /translation="MEKLSVVIISFNEEKNIDRCLRSVKGIADEIVVLDSFSSDRTGA
                     IVKEFGGHFYQQAFTGYGAQKNAASALATHDHILFLDADEFLSEALAGQILREKDGGF
                     PYDGYSMNRLNNYCGRWIRHGSWYPDKKLRILHRQKGAWSLDIVHESLIPDTGARISH
                     LPGDLLHYAYSSIEEHTEKNNRYSTLSALLLYGKGKRTRRSRILLNPFWAFIQSYLLR
                     SGFLDGFFGLTIAIQIAHLTFLKHVKLYQLQKSK"
     gene            8254..8601
                     /locus_tag="VGM24_03510"
     CDS             8254..8601
                     /locus_tag="VGM24_03510"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY4154459.1"
                     /translation="MPQHCPVCGQRFELEIGFWYGTAYVSYLLTVIFSGISFAVWWLT
                     IGFSLVDNRFFWWLAINGVLLVVLQPWFMRLSRALYLYFFVSYDENYESAPTKTFDYS
                     SEDYFLKKEDPVK"
     gene            complement(8711..9574)
                     /locus_tag="VGM24_03515"
     CDS             complement(8711..9574)
                     /locus_tag="VGM24_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4154460.1"
                     /translation="MNLYPIQAGYFKLDGGAMFGVVPKSIWNKTNPADPDNLCSWAMR
                     CLLVEADGRLILVDNGIGDKQDAKFFSHYHLHGDDGLEASLKKNGFRKEDITDVFLTH
                     LHFDHCGGSIVRSGGQLLPAFPNAKYWSNQAHWLWATEPNDREKASFLKENILPIKES
                     GRLNLIPVPLKPETNHLPAAEFLPGFHTRFVNGHTAAMMLPQLEYKGRTIVYVADLFP
                     SAGHLPVPYVMAYDMFPLTTLQEKKQFLEEAAENDYVLFFEHDPENECCTVQRTEKGI
                     RVKDLFRLKDL"
BASE COUNT         2511 a         2368 c         2366 g         2358 t
ORIGIN      
        1 aaagccgtgg gcttgtatcc cagggccttc tcgaaaagca gggtaatatt ccggatcttt
       61 tccgattcaa tgtccggcgg gtagaaacag cagttggcaa cccctttctt tcccgcatag
      121 ttttcatatt ttttatcggg caatatgaat tccgggtgaa ggtgcgtgcc cagctcgaac
      181 tttccctcca ggtgaagaaa acagctgacg ctgctttcat cttccaatac cacattattg
      241 accaggtacg tggggctgat accctgttta ttgaaaagag gttgcagaat ttgcgcaatc
      301 ccctccgaaa cgcccttgaa cgtaagcgga tcggcgtagt gccaggtagg agaacagtcg
      361 ggttcaacat caatcgtaat gacaagatat ttcttcatat cggccgcaag ggggagatct
      421 ttttgaattt ttgattccag agcgccaggt tcagcaaata ccagacaaca atggtgaatt
      481 tcttgtcgcc accataatat ttttcaacca tgggaagata tcgtttatca tccaggccca
      541 gcgaagctac actttcttta tttagagacg agagcacctt gtccttccag gcatggcgta
      601 gccatttgtc cagcgggaaa ttgaaccctt tcttgggccg gtctatcgga taatgcggcg
      661 cgagcttttg cagcaattgt ttgagtatgg ctttatgttt aaactgaggg ttggatttat
      721 aaataaaggg caacgccagc gcataatcga ttaccgtatt atcgaggtat ggcacccgca
      781 cttccaggct cgcggccatg cttgcccggt ctactttgta caacatcgcg tcggccaggt
      841 aggtcttaat atcgaggacg gcggccaggt cttcccggtc ccgtataagc acagtgtgtt
      901 ccagggccca gaaatcgtcg gtccagaccg gatgctgcag caggtggccc aaagcaggcg
      961 aaaggaaaag gctgaagtga cgccgtaccg ggtcggtctc cagttccagg gcatatttcg
     1021 cgcgccagta tttccccggt agaaaagggc gtgccagcga tgccagattt cccgggatat
     1081 tcagcttgtt gaacaggtgc aaggaaggat ttttccattg gtcgtatctg gggtaacccc
     1141 agaacaattc atccgccccg tctccggaga gcgctacggt caccgattcc ctggctttgg
     1201 cggaaattac ccaattcaac agcgatgccg tgtcgccaaa gggctcatca aaataatcgt
     1261 acaggagttc cagcttttca ccgacgagct tcgcatcgat ggaaagctta tggctccgga
     1321 gcccgggatt atggaagccc cggataaagg ctatggcatc gtcgctttcg tctgaagcag
     1381 tgatttcatt aaacgcaacg gtgaagaaat taaatgcctg cgtgcccgca aaatgcttgt
     1441 tcaccaaagc cgccagtagg gatgaatcca ctcccgagct gagaaaaagc cccagcggaa
     1501 catccgcttc catctggcgg gagaggctgt caaccagtat attttccagt tgctccgggt
     1561 cggcatccgg cgctccggag gctattttcg aataggcatc ccagtaaggt tcagccgcgg
     1621 gcgcctgatg ccggtcgagc gattcccagt ttccttccag gtaatgcccg ggcggcagtt
     1681 tgaaaatgcc ctgcacgatg gtatttggcg acggaatgaa ttgcatccag agaaactggc
     1741 ggacggactc cgggcggatg ggtggcaatc cgtccgacag gcgcaaaacg gaatccagct
     1801 ccgatccgaa aataaaggtc ttcccctgct gtttatagta aaccggcttg atgcccgtgc
     1861 ggtcgcgggc tattatgaat ttcttctctt tccggtctat ccagcagaag gcaaacatac
     1921 cgttgagccg gttcagacct ttcgagcccc atttcaccag aacatgaaac aatacttcgg
     1981 tgtcggagct agtatgaaaa aggacgcctt ccttttgaag ggtctttttc agttcgcggt
     2041 aattgtagat ctcgccgttg aacagaagtg aataccgctc gtcttcgaaa ggctggtttg
     2101 atcttggatc gagatctatg atggcaagcc ggttaaagaa cagggcattg ttcccgtcta
     2161 ccacaggatg aagatgctgg tcgtagagac gcaggccgca atcgtcgggt ccgcggaaac
     2221 agactgcagc agcgatgccg gccagttgct gtcgcctgac gggaaaagca ttattcaata
     2281 ttccggcaaa accgcacatt cactttttat tattttttta tcagccatat ctggcgctcg
     2341 cgttcgtagt cgcccagctt gcttacgccg gctccgatgc catctgttaa aaaccggacg
     2401 tcgtgattat attcatattc cgggtcctgc accgtatcga taatgatgac gcccccttcg
     2461 ttcagggcga cgttgaaata gtccgcaatg ctggatctta ataccgccgg ttcgatatga
     2521 tgtatccagt tcaccaaaac aatcacatca taatttcccg gcaggggcga accggggaag
     2581 gtaaaacccg aaaaccggat ctttccaccc cccataatgg aattcaaaaa acgggcggcc
     2641 tttaacgctt tttcgtccag gtcatacccc tgcctttccc ggtaatacac atggcgcacg
     2701 atatcgccca gcccgcaacc gatctcggca aaccggttcc tgaccgggcg ggaattgcaa
     2761 tatgcgatga tgtctttggc atactgcttt tcggtgaggg taaaaacatg ccatttgtca
     2821 aatttgaaaa tataccgcaa aataatccgc tgcagcttcc ctccccagac ctttaatttc
     2881 cagaccagtt tttccattgt caaatattct tggctgcaat tgaaatgatg tagggggaaa
     2941 atcgcctggt gggtatattt aattttaaca gccggaacgt tctgctggcc agtttatcaa
     3001 agacgctaaa accggataag gcaatctttc tttgtgtgct accctcccgg agcagccagt
     3061 gcgtgcttcg gatcaggggc aatacggcaa gggtaaatgt gccggcatag tttgttttaa
     3121 gattatgcaa cgcattgctt tcgtgatatc gctgcagctg ctgcctgctg atgacgcggt
     3181 gcatcttata aatatccggt ataaacagcc tggaaagcca tcccatggac ccgttcaggt
     3241 taggaacgag cgtgatcatg aggccgccgg gattcaggaa agacgaaaaa atgcggatga
     3301 tgtcttccgg gttttcaaaa tgctcgatga ccccgtaact gaaaacaaca tcgtattttt
     3361 ttccgtccgt acagttgttt tcaaaaatat cccggcaaat gatttctcca taaggaattc
     3421 ccaaaatccg caggttctct tccgcgagcc tgcacccggt ggcggaataa tcgatcccgg
     3481 aaacggtcag gccatattct tgtccgaaat aaggcagcca gcccgaggag ccgcatccca
     3541 cctcaaaaaa tgaggtatat ggcttattgc gcaggaaggg atcgatgaaa tccatggtga
     3601 cgcggtaatg gtaagaacgg cggctgttga tccttggcaa ttttgcttct tccagaacaa
     3661 ggtcccagta cgcctgggaa gaaagttttt cagcggtttt tagttcttcc atagtttaat
     3721 ccttttctcc agaattcacg aacaaaatca ttgaaggtgt cgtttatatt ggatttaaaa
     3781 ccaataacaa atataaggct taaataaagg ctcgccccca cggccagcga caggaatccg
     3841 gattgaaagg ccgccttgga aataaacaaa taaatacctg cccccaccac cccgttaacg
     3901 accagcagca tcagcatatt cttaagcacc tgtgcgtact ggatcgtgcc aaaatacttt
     3961 ttctcaagaa aataacagcc gagtatattc aatacgtgta cgcataccag ggttatgggg
     4021 taggcggcaa acccgaaacg atggatgctc aaatacaggg tggcaatgag cacgatattg
     4081 aagcaaacct gataccagaa cgattccatg atcttatggc tcgccatgaa caggcgcgca
     4141 aacagggtat tgatgaccag catcggcagc agcaggccga ggtattgcag gaataacgag
     4201 gtataggcga caccctgctt tccgaatgcg ccatgctcca gtaatatgga aacgatttgt
     4261 gaagggaaca gaaaaaaaat accggcgacc gggaataaaa taaacatcag gaaattcgcg
     4321 gtcgacagaa aagtgctgtt gagcttcccg aaatcctttc ccgcgtagga ttcattgaat
     4381 ttgattcctg ccacggcaga aaactggttg gtgatcagcg tggtgggaag ggaggagatc
     4441 tgctgggcaa aattcagcga ggtgatgatg ccgccgttga aaccgctcag cagatacaaa
     4501 ggggcatacg cgctgaggga actggtgatg tttccggcct gcgcaaaagc gatattcttc
     4561 cagatccttt tttcaatttt caaccggtga aaaccgaaag tccagttcag tctctttttc
     4621 atgagggaaa ccagcaggat gaaattcagt gtgtaggaaa gcagcaggcc ggcgagcagg
     4681 ctggaaacat ccagcaggcg gtggaacaag acaataaaaa ggatagaaaa tattccattg
     4741 atgatgccgg caatcatcgg aatggaaaaa aatttatacg aagtcatgat atccgtgagc
     4801 aggttagtta cgggcatcag gaagataagc ggaaccgcca ggtataatat cctcgcctgt
     4861 tgttccagcg attccctgga aaaaccggat accgcgtgca gggcactaac cgggtttatg
     4921 aggaacaaca ggcagacccc gatggtgatg gcggcataca ggtaaaggaa aaaattcaga
     4981 aaaaacatgc cccggcgctc gctctcctgc gcccggaggc gcatcgattc ggggatcagc
     5041 accgatgaat tgaggcttgt gatgaaggcc gccaggatta cgatggtatt gtaggcataa
     5101 aagtaaatgt ccattttaag ctgggcgccg aaaaaataag cgatgatcag gctattgcaa
     5161 aaaaccaggc ctttattgag aatattaaaa acggtagata agacaattcc tttccggtat
     5221 gattcgactt tcagcaaaac atcggggttt atcaatcaac aaataatctt tcaactgaaa
     5281 gatatacaat gcggtaaatg taacgaatca tacccaccgg gtgaaaacaa attagccatt
     5341 tgcgccggct ggtaaacccg actgatatgc tgtgccggcg ggctaaaaga aactcttccg
     5401 gcaggtgccg ggccggtaaa tatttatccg tgccctgacc gtatcttgtc ttaatttccg
     5461 aacttgtata ataatgccgg ttaaaacatt tctcattccc ggatgttatt caactcaata
     5521 tgaaaatcct gctcacctat ttgaagccgt acaaaatgct ggtggccctt acgctcttcc
     5581 tggccagcgt caatatcggc ttttcgctgt tcgaccccat catattcgga aagatcgtga
     5641 acctttccaa tgaatatgtg caggcccgca aacttcacca ggatttttcc gcggatcatt
     5701 ttttcactgc tttttcctgg acccatccgg gggtgatcaa tttattgctc ctttccatcg
     5761 gggttgccat ggtgagccgg ctggctaaaa atttccagga ttattttatg aacgtcgtgg
     5821 tgcagaaatt cggcgccaag gtatttaccg acggattgca gcatgctatg aaactgcctt
     5881 accaggactt cgaagaccag cgcagcggag aaaccctggg ggtattgcag aaagtccggg
     5941 aagataccga acggttcatc aactacttca tgaatgtgct tttcggggtg atcgtcgggg
     6001 tcattttcgt tttcacctat gcagccatct ggatcaatgt gagcattccc attgtctatc
     6061 tcgcgggcat cctgctgctc acgttcatca gcaatgtgct gagcaggaag attaagagca
     6121 tccagaaaaa catcgttaac cagaccactt ccctggccgg atccacaacc gaatctctcc
     6181 ggaatatcga actggtgaaa agtctgggtc tcacccagga agaagtcacc cggctgaata
     6241 aaaacaccta taaaattctc ggcctcgagc tcaccaaggt aaaacgcgta agaagcatca
     6301 gttttatcca gggaaccttt gtgaacacct tgcggcaggt gatccttttc attttaatgt
     6361 ggctgatatt caaggataaa atgaatgcag gtcagttggt gacgatgcag attttttcct
     6421 tctttgtatt cgggcctctg caggatatcg gcaatattat catgtcctac cgcgaagcgg
     6481 aggcttccct taataatttc gacgacctga tgaagaaggt tcccgaaccc cagtctgcca
     6541 atccgaaaca cctcggcaaa attgagcagc tcagtttccg ggaggttatt ttcaagcata
     6601 agtccgttca gcattatgcc attgaccgga tcagttttga tgttcgggta ggggaaacca
     6661 ttgcttttgt cggcccttcc ggtgcaggaa aatccaccct gatgaagctg ctggtcgggt
     6721 tataccggcc catgggaggc cgtatcctgt acaatcacct ggatgaaaat tctattgact
     6781 tcgacgacct gcgcaaccag atcggtttcg ttacccagga tacccagtta ttttccggaa
     6841 ccatccgcga aaatctcctg ttcgtaaacc ccaaagcgac ccaggaagat ctgctggatg
     6901 ttttacaaaa agcttcctgc aacggcctgc tgcaaagagc ggaacacgga ctggaaacgc
     6961 ggatcggcga gggcgggtta aaattatccg gtggtgaaaa acaaaggata tccattgccc
     7021 gggccctgtt gcgcagaccc cgggtgctca ttttcgatga agccacgtcc tccctggatt
     7081 cattaaccga agaagagatc accagcacca tccggaaagt ttcctcccag aaaaaccaga
     7141 ttacgatttt gatcgcccac cggctgtcta ccattatgca cgccgaccgg atctacgttc
     7201 tggagaaagg ggaagtggtg gaaaccggaa cgcataaagc actgctcgat gaaaagggtc
     7261 tgtattatgc catgtggcgg caacagattg gagagagaaa aaacagccag gatattccgg
     7321 aaaccgaaat gagtgtcatc cgctaaattc cgatggaaaa actatccgtt gttatcatca
     7381 gttttaacga ggaaaagaat atcgaccggt gcctgcgctc tgttaaaggc attgccgatg
     7441 agatcgttgt gctggattcc ttttccagcg accgtaccgg agccattgtg aaggaattcg
     7501 ggggccactt ttaccagcag gccttcaccg gctatggcgc ccagaaaaac gccgcctccg
     7561 ccctcgccac ccacgatcac atcctgttcc tggatgccga tgaattcctg agcgaggccc
     7621 tcgccggtca aatcctccgg gaaaaagacg ggggatttcc atacgatgga tacagcatga
     7681 accggctgaa caattattgc ggtcgttgga tccggcatgg cagctggtat ccggataaaa
     7741 agctccgtat cctccatcgt cagaaaggcg cgtggagcct ggatatcgta catgagtccc
     7801 tgatcccgga tacgggagcc cgtatcagcc atctgccggg agaccttttg cactatgcct
     7861 attccagcat agaagagcat accgaaaaga acaaccggta cagcacgctt tcagcccttt
     7921 tactctatgg aaaagggaaa agaacccggc ggtcccggat ccttcttaac ccgttctggg
     7981 cctttattca aagctatctg cttcggtccg gtttcctgga tggattcttc gggctgacca
     8041 ttgcaatcca gattgcgcac ctgaccttcc tcaaacacgt caaattatat cagctgcaga
     8101 agtcaaaata attgcgttta cctttaccga aataatccta tggctggcaa acccggaatg
     8161 atatggagcg ttctctcaca gaaatgtccg cattgccggc gtggagcgat gttttccaac
     8221 cccaatccct ggaaccttaa ggaaatgacc aaaatgcccc agcactgccc cgtctgcgga
     8281 caacggtttg aactggagat cggattttgg tatgggacgg cctatgtcag ctatctgcta
     8341 acggttatct tttccggaat aagtttcgcg gtctggtggc tgacgattgg tttttccctg
     8401 gtagataacc ggttcttctg gtggctcgcc attaacgggg tgctgctggt ggttctgcaa
     8461 ccgtggttca tgcggctcag ccgggcgcta tacctttatt tcttcgtgtc ctacgatgag
     8521 aattacgagt ccgcacccac taaaacgttc gactattctt cggaagatta ttttctcaaa
     8581 aaagaagatc cggtcaaata gaagcaataa aaaaagccag gaaaaaattc ccggcctgtt
     8641 gaatctatga taggggttag taagcctcta taacgcaaaa ctataaagaa tattatatat
     8701 tccggcggat tcaaagatct tttaaccgga ataggtcttt tacccggatg cccttctcgg
     8761 tccgttgcac ggtacagcat tcattctccg ggtcatgctc gaaaaacaga acatagtcat
     8821 tttccgcggc ctcttccaaa aactgttttt tttcctgtag cgtggtcagg ggaaacatgt
     8881 cataagccat tacataagga accggcagat ggccggcaga gggaaaaaga tcagccacat
     8941 aaacgatcgt tcttccctta tattccagtt gcggtagcat catggcggca gtgtgtccgt
     9001 ttacaaaccg ggtatggaac ccgggtaaaa attcagccgc gggcaaatgg tttgtttccg
     9061 gtttcagcgg aactggaatc aggttaagcc ggccgctttc cttaatggga agaatattct
     9121 ctttcaggaa agaggctttc tcccggtcat tgggctcggt ggcccacaac caatgcgcct
     9181 gattgctcca gtattttgca ttcggaaagg ccgggagcag ctgcccgccg ctgcggacta
     9241 tgcttccacc gcaatgatcg aaatgcaaat gcgtcaggaa aacatccgta atgtcttcct
     9301 tccggaatcc attttttttt aaggaggcct cgagcccgtc atctccgtgc aggtgataat
     9361 gactgaaaaa tttagcatcc tgtttatcgc caatcccatt gtccaccagg atcagcctgc
     9421 cgtctgcctc aaccagcagg caacgcatgg cccaggaaca gagattgtcc ggatccgccg
     9481 gattggtttt attccagatg cttttaggca ctacgccgaa catggcgcct ccgtcgagtt
     9541 tgaagtatcc ggcctgaatg gggtataggt tcatggaaca aaattaacgg attgatttcc
     9601 ttt
//
LOCUS       DASYCU010000172         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2940796, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000172 DASYCU010000000
VERSION     DASYCU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2940796"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /gene="folK"
                     /locus_tag="VGM24_03520"
     CDS             <1..461
                     /gene="folK"
                     /locus_tag="VGM24_03520"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY4154461.1"
                     /translation="TGGNEGNKYLNMQLARTNIEHICGQLLQVSSLYETAAWGKTDQA
                     DFLNQVLLINSSLAPQDLLRANLHIEESMGRIRTVKNAPRTIDIDILFYDQEIISTPD
                     LMIPHPRIAERRFVLEPLNEIAAGFIHPVLGKTVRELLRECTDTLPVKKI"
     gene            506..1138
                     /locus_tag="VGM24_03525"
     CDS             506..1138
                     /locus_tag="VGM24_03525"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY4154462.1"
                     /translation="MNHHFIAVEGNIGAGKTTLAHLLARHFNSRLILEAFAENPFLPK
                     FYENPKQYAFPLELFFMAERYKQLKELVHTKDLFQSLTISDYIFSKCLLFAKVNLPDE
                     EFHLYQKLFDIIYQQLVQPDLLIYLHSPVSQLQKNIRKRNRAYEQSIPDNYLFSLQET
                     YTSYIRQHNIKTLFIDAANADFLGNEKHFQIVLEALEKDYEPGQHYIALP"
     gene            1164..>1974
                     /locus_tag="VGM24_03530"
     CDS             1164..>1974
                     /locus_tag="VGM24_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4154463.1"
                     /translation="MENESLSSSPLEAVRIFEFRNLVIGRFLFIMGLRMMSTLVGWWI
                     YNLTNAPLAIGIVGLSEFVPAVSFALYAGHVIDLSEKRKMMLTGVSLYGLAALLLLIL
                     SSRIGSNHLHPHSIALLIYLIIFGTGVVRSFTGPIFNVLLAQVVPKKLLQNATTWNQG
                     AYLSASVTGHALGGFLIAGLGNTGTLTVVAILVFAAFSVLFTLKPKPPMNERGEKKTW
                     DSVREGLAFVFRTKELLGALSLDLFAVLFGGAVAMVPVYARDILKVGAQGFG"
BASE COUNT          502 a          484 c          462 g          526 t
ORIGIN      
        1 tgacaggcgg caacgagggc aacaaatacc tgaatatgca gctggcgcgg acaaatatag
       61 aacatatttg tggtcagctt ctacaagttt catcactata tgaaacggca gcatggggaa
      121 aaacggacca ggctgatttc ctcaaccagg tcctgttaat caacagcagc ctcgccccgc
      181 aggatctcct gcgggcgaat ctccacatcg aagaaagcat gggcagaata agaacggtga
      241 agaacgcccc gcgaacaata gacatcgaca tcctttttta tgatcaggaa atcatttcaa
      301 cgccggatct catgatcccc catccccgga ttgcggaacg ccggtttgta ctggagcctc
      361 tgaatgaaat tgcggccggc tttattcatc ccgtattggg gaaaacagtg cgggaacttc
      421 tgcgggaatg tacggatacc ctcccggtaa aaaaaatatg aacagtccgg gataacacaa
      481 atggaatccg gatatttaca gcttcatgaa ccatcatttt attgccgtgg agggaaatat
      541 cggggcggga aagaccacgc ttgcccatct gctggcgcgc cattttaatt cacggctgat
      601 cctcgaagcc tttgcggaaa atccttttct tcccaaattc tatgaaaacc ccaaacaata
      661 tgcttttcca ctggagcttt tttttatggc ggaacggtat aaacagctga aggaacttgt
      721 acacacaaaa gacctttttc aaagcctgac catttccgat tatattttca gcaagtgcct
      781 gctttttgct aaagtgaatt tgcccgatga agaattccac ctgtatcaga aattatttga
      841 tatcatctat cagcagctcg ttcaacccga cctgttgatt tatttacatt cccctgtcag
      901 ccagttgcaa aaaaatatcc ggaaacggaa ccgggcttac gaacagtcca taccggataa
      961 ttaccttttt agtttgcagg aaacgtatac cagttacatc cggcaacaca atatcaaaac
     1021 attattcata gacgcggcca atgcggactt cctcggaaat gaaaaacatt ttcagatcgt
     1081 cctggaagcg ctggaaaaag attacgagcc cggacaacat tatatcgctt taccctgatc
     1141 gtcctgtaat atctttgcaa gcaatggaaa atgagtcgtt atcttcctct ccgctggaag
     1201 cagtccggat ttttgaattc agaaatctgg tcatcggccg ttttttattt atcatgggat
     1261 tgcgcatgat gagtaccctg gtcggatggt ggatttacaa cctcacgaat gccccactgg
     1321 ccatcggcat cgtcggcctc tcggaatttg tgcctgccgt ttcttttgcc ctctatgctg
     1381 gacatgtgat cgacctgagt gagaaacgaa aaatgatgtt aaccggggtg tctttgtatg
     1441 ggttggcggc cttgctcctg ctcatccttt cgagccgcat cggaagcaat catcttcatc
     1501 cgcattccat tgcgctgctg atctacctga ttattttcgg gaccggtgtg gtccgctcgt
     1561 ttactgggcc tatattcaat gttctgcttg cccaggtggt tccaaaaaag ttattgcaaa
     1621 atgccaccac ctggaaccag ggtgcttatc tttctgcatc ggtaaccggt catgcgctgg
     1681 gcggtttcct gatcgccgga ctgggaaata ccggcaccct gacggtggta gccatccttg
     1741 tatttgccgc cttttccgtt ttattcaccc taaaaccgaa gcccccgatg aatgagcggg
     1801 gggagaaaaa aacctgggac agtgtcaggg aaggactggc ttttgtcttc cggaccaaag
     1861 aattgctggg cgccctttca ctggatcttt ttgcggtatt gtttggcggc gctgttgcca
     1921 tggtaccggt ttatgcccgg gacattttaa aagtaggcgc gcaggggttc ggat
//
LOCUS       DASYCU010000173         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2359387, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000173 DASYCU010000000
VERSION     DASYCU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359387"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGM24_03535"
     CDS             <1..691
                     /locus_tag="VGM24_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY4154464.1"
                     /translation="PAAYQLVSEANDKSVFSFCMCPGGIIAPAATREGELVVNGWSPS
                     KRNNPYANSGIVLNIENSDWQKFSKHGPLAAMYFQSSIEQQAFQAGGGLFKAPAQRMT
                     DFVHNKISASLPACSYVPGLTSASVAGVFPSALARSLQKAMPGFGKKMKGYYTSEAVV
                     VAAESRTSSPVRIPRDPGSLAHPQLENLFPCGEGAGYAGGIMSAAMDGERVAERIASI
                     FSLKQTVHVNA"
     gene            678..1610
                     /locus_tag="VGM24_03540"
     CDS             678..1610
                     /locus_tag="VGM24_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4154465.1"
                     /translation="MSMLEVFHLKKYFATQKAVDDISFKVEKGTIFGLLGPNGAGKTT
                     LIRMITGIFYPDEGNIQLDGKKFNPDQDASRIGYMPEERGLYKKMKIGEQAIYLAQLK
                     GLSRSEAIARIKKWFTRFEMESWWNKKIEDLSKGMAQKLQFVITVLHEPELIILDEPF
                     SGLDPINANLIKDEIFKLAANGSSVIFSTHRMEQVEEICNHIVLINKGQKILDGTIAG
                     VKQEFKENLFRITARPLPEKISDSLFDLLAENDHTLTLRIKDGHSPNEVLGYFIDQKS
                     NISNFMELLPSLNEIFIRLVEGTSVSRQFQNVQS"
     gene            1615..>2288
                     /locus_tag="VGM24_03545"
     CDS             1615..>2288
                     /locus_tag="VGM24_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154466.1"
                     /translation="MQKIGLIIRREYITRVRNKTFLLSTFLLPIVIVLLISGATFLAI
                     KGKTSHRIAVLDQNGYFKDYLRSDSSIIFDFSPGIDSINYPAKNCSAVLLIPVLKEGQ
                     KTVCRLKYKKQLGLSNIDDLEKRIGSAVTDHLIYTKTNLSRSRLDSIRAESDMAELKT
                     FSDEGKKAKASSQGLAYGIGYACGFLIYLLTFIYGAAVMRGVMEEKTSRVAEVVVSSV
                     KPFPLML"
BASE COUNT          601 a          590 c          571 g          526 t
ORIGIN      
        1 gccggccgcc taccagctgg ttagcgaagc gaacgacaaa tccgtctttt ccttttgcat
       61 gtgccccgga gggatcatcg cgcccgcagc cacccgggaa ggagaactgg tcgtgaacgg
      121 ctggtcgcct tccaaacgga acaacccgta tgcgaattcg ggtatcgtac tgaatataga
      181 aaattctgat tggcagaagt tttcaaaaca cggaccttta gctgccatgt attttcaatc
      241 ctccatagaa cagcaggcat tccaggccgg aggcggtctg tttaaagcgc ctgcccagcg
      301 gatgacggat ttcgttcata acaaaatctc cgcgtcctta cctgcctgct cgtatgtgcc
      361 cggactcacc tccgcttccg tcgccggcgt gttcccctcc gcgctggccc gaagcctgca
      421 gaaagcaatg cccggattcg gtaagaaaat gaaaggatat tatacttcgg aagcggttgt
      481 ggtggcagcc gaatccagga cttcctctcc cgtacgcatt ccccgggatc ccggaagcct
      541 ggctcatccc cagctggaaa atttatttcc ctgcggggaa ggcgccgggt atgccggggg
      601 aatcatgagc gcggccatgg atggggaacg cgttgccgaa cggatcgcca gcattttttc
      661 actcaaacaa acagttcatg tcaatgcttg aagtgtttca cctgaaaaaa tattttgcca
      721 cgcagaaagc cgtggatgat atcagtttca aggtggaaaa aggaaccatc tttggattac
      781 tcggccccaa cggcgccggt aagaccacgc ttatccggat gatcaccggt attttttatc
      841 ccgatgaagg aaatattcag ctggatggaa aaaaattcaa tcccgatcag gatgcgtccc
      901 ggatcggata tatgccggaa gaaagaggcc tgtataaaaa aatgaagatc ggggagcagg
      961 ccatttacct cgcacagcta aaagggttgt ccaggtccga agccattgcc cggataaaaa
     1021 aatggtttac gcgttttgaa atggaaagct ggtggaataa aaaaatcgag gacctgagca
     1081 aaggaatggc gcagaagctc cagttcgtca ttaccgtttt gcatgaaccc gaactgatca
     1141 tcctggatga acccttcagc gggctggatc ccatcaacgc caacctgatc aaggatgaga
     1201 ttttcaagtt ggcggcaaac ggatcttccg tcatcttcag cacccaccgg atggaacagg
     1261 tggaggagat ctgcaatcat attgtgctga tcaataaagg ccagaagata ctggatggca
     1321 ccattgccgg ggtgaagcag gaattcaagg aaaacctctt ccgcatcacg gcccggcccc
     1381 ttccggaaaa gatcagcgat tccctgtttg acctcctggc cgaaaatgat catacgctta
     1441 cgctgcggat aaaagacgga cattctccca acgaagtact gggatatttc atagatcaga
     1501 agagcaatat ttcgaatttt atggaattac ttccctcgct gaatgaaatt tttatccggc
     1561 tggtggaagg cacttcggtt tcccgccaat ttcaaaacgt tcaatcctaa tcgcatgcaa
     1621 aagatcggtc tgatcatccg ccgggaatat attacccggg tcaggaataa aacattttta
     1681 ctgtccacct ttctcctgcc catcgtgatc gtgctgctga tctccggcgc aacctttctt
     1741 gccataaagg gaaagaccag ccaccgcatc gcggtgctgg atcagaacgg ttatttcaaa
     1801 gactacctca ggagtgattc cagtatcatt tttgatttca gcccgggcat tgacagcatc
     1861 aactacccgg caaagaattg ttccgccgtt cttcttattc ccgtactgaa agaagggcag
     1921 aaaacggtct gccggttaaa atataagaag cagctggggc tatcgaatat tgacgacctg
     1981 gaaaaaagaa tcggttccgc ggttacggac cacctcatct acaccaaaac aaatctcagc
     2041 cgcagccggc tggattccat ccgcgccgaa tcggatatgg cagaactgaa aactttttcc
     2101 gatgaaggta aaaaggcaaa ggcttccagc cagggccttg cctatggcat cgggtatgcc
     2161 tgcggattcc tcatttacct gctcacgttt atttacggcg ccgccgttat gcggggcgtc
     2221 atggaagaga aaacaagccg ggtggccgag gtggtcgtca gttcagtgaa accctttccc
     2281 ctcatgct
//
LOCUS       DASYCU010000174         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4535877, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000174 DASYCU010000000
VERSION     DASYCU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4535877"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1704)
                     /gene="ftcD"
                     /locus_tag="VGM24_03550"
     CDS             complement(40..1704)
                     /gene="ftcD"
                     /locus_tag="VGM24_03550"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY4154467.1"
                     /translation="MAAIIECVPNFSEGRDLRIIQAITNEIESVEGIRLLNVDPGKAT
                     NRTVVTFVGEPPAVLQAAFLAIQKAGELIDMRNHKGEHPRMGATDVCPLIPVSGISLE
                     ETADWAKKLAVRVGKELSIPVYLYEAAQADKSRSNLSVIRAGEYEGFPEKIGRAEWEP
                     DFGPAVFDLRRGATVIGARDFLVAYNVNLNTTSVRRANSIAFDVREAGRVKREGDPLT
                     GKIVKDPQGIPVNIPGTLKSVKAIGWFIEEYGMAQISMNLTNLSVTPLHIAFDEVCRK
                     AESRGIRVTGSELVGLIPLQSLLDAGKYFLRKQKRSAGVSEKELIRTAVRSMGLDELG
                     PFKPEEKVIEYLLGNESAGKMIRLSLADFADETASESPAPGGGSVAAYLGVLGASLAG
                     MVANLSAHKKGWDDRWLEFSNWAERAQESKNALLRLVDADTRAFQELMQAHALPRSTA
                     EEKEIRERATAKATAKAIEVPFEVMERALESMQLIKTMAREGNPNSVSDAGVGALCAR
                     SAVLGAYLNVRINAAGSEPDEKISGILSKAKKMEAEARALEEDFFI"
     gene            complement(1780..3030)
                     /gene="hutI"
                     /locus_tag="VGM24_03555"
     CDS             complement(1780..3030)
                     /gene="hutI"
                     /locus_tag="VGM24_03555"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY4154468.1"
                     /translation="MPTRILTHIKQLLNVRADTKPLYGSELAGMPDLEDAYLIIEDGE
                     IAACGTMSSWKPSFRMPADKLDCSGRLVFPCWCDSHTHLVFTGSRENEFVDRIRGLSY
                     SEIHARGGGILHTVQRVREASEEELFNESWKRLEEVSRLGTGAIEIKSGYGLTVESEL
                     KILRVIRKLKQKSSLSVRSSFLGAHTYPAAYREDHPGYLRLIIDEMLPVIAREKLADY
                     IDVFCENGFFSPEETETILRAGLRYGLQPKLHSNQLHSIGGLQTGIKMNAVSVDHLEI
                     ISEEDMESLSRSGWKGFCTLLPTAAYFLGMRLPPARDLIRAGCALALASDYNPGSSPG
                     GNMNQVVSLSCIQMNLLPEEAVNAATGNGAYAMGLGDVCGSITPGKRANLIVSLPVPS
                     LAYLPYAFGKNHIDRVMIGGEFIS"
     gene            complement(3041..>3407)
                     /locus_tag="VGM24_03560"
     CDS             complement(3041..>3407)
                     /locus_tag="VGM24_03560"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218468.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY4154469.1"
                     /translation="RSKKLKAPVVIGRDHLDTGSVASPNRETEAMLDGSDAVADWPIL
                     NALVNTAGGASWVSLHHGGGVGMGYSIHAGMVIVADGTDAAAARLSRVLRNDPGLGVI
                     RHADAGYETARETKNKFNL"
BASE COUNT          734 a          972 c          872 g          829 t
ORIGIN      
        1 aaattcaaac acacaaaaca aacaacaccc taaaaacaac taaataaaaa aatcttcttc
       61 cagggctctg gcctctgctt ccattttttt tgccttgctg aggataccgg agatcttttc
      121 atccggttcg gagccggctg cattgatgcg gacattcaga taagcaccca gtacggcgct
      181 gcgggcgcaa agggcgccga ccccggcatc ggaaacggaa ttcggattgc cctcccgggc
      241 catggtcttt atgagctgca tggactccag tgcgcgttcc atgacttcaa atggaacttc
      301 gattgctttt gccgttgcct ttgctgttgc cctttcccgg atttcttttt cctccgccgt
      361 agaccggggc agcgcatgcg cctgcatcag ttcctgaaat gcccgggtgt ccgcatccac
      421 cagccgcaat aaagcgtttt tactttcctg cgcccgttca gcccagttgc tgaattccag
      481 ccaacggtcg tcccagcctt ttttatgtgc ggacagatta gctaccatgc cggcaagaga
      541 agcgcccagc acgcccaggt aagcggcaac agatccacca ccgggagccg gactttcact
      601 tgccgtttca tcagcaaaat cggccaggga caagcggatc attttcccgg ccgattcatt
      661 tcccaacagg tattctatga ctttttcttc cggcttgaac ggtcccagct catccagccc
      721 catggagcga acggctgtcc ggataagttc tttttccgaa actcccgcgg atcgcttctg
      781 ctttcttaga aaatattttc ccgcgtccag cagggactga aggggaatca gccccaccag
      841 ttcgctgccc gttacccgta tgccccggga ttctgccttg cggcatactt catcaaaagc
      901 aatatgcagg ggggtgaccg aaagattggt caggttcatg gaaatctgtg ccatgccgta
      961 ttcttcaatg aaccagccga tggccttcac cgattttaag gtgccgggaa tattcaccgg
     1021 tatgccctgc ggatccttta cgatttttcc ggtcagcgga tctccttccc tttttaccct
     1081 cccggcctcc cggacatcaa acgcaatgct gttcgcccgg cgcacggacg tggtattgag
     1141 gttcacattg taagcgacca ggaagtcccg ggcgccgatc accgtggctc cgcgacggag
     1201 atcaaatact gccggaccga aatcgggttc ccattccgcg cggccgattt tttccggaaa
     1261 tccctcgtac tcaccggcac gtataaccga taagttgctc cgcgacttgt ctgcctgtgc
     1321 cgcttcatag agataaaccg gtatggacaa ttcctttcca acgcggacgg ccagtttttt
     1381 tgcccagtct gctgtttctt ccagggagat cccggagacg ggtatgagcg gacagacatc
     1441 ggtggcgccc atccggggat gttctccctt gtgattgcgc atatcgatga gttcgccggc
     1501 tttttgaatg gcgagaaagg ccgcctgcag cacagccgga ggttctccga caaaagtaac
     1561 caccgttcgg ttggtggctt ttcccggatc cacattaagc aggcggatgc cttccaccga
     1621 ttcgatttca ttcgtaatgg cctgaatgat gcggaggtcg cggccttcac taaaattggg
     1681 aacacattct attatggctg ccataaccta tatctgggtc tacaatgtta aggaaagaaa
     1741 atcaatccgg cttcaggtac aggccgtata tccgatcctt taagaaatga attccccccc
     1801 gatcattacc ctgtcgatat gatttttacc aaaagcataa ggaaggtaag cgagtgaagg
     1861 gacgggtaaa ctgacgatga gattagcccg tttaccggga gtaatgctgc cgcagacatc
     1921 gcctaatccc atggcgtatg cgccgttccc ggtggcggcg tttacggctt cctctggcaa
     1981 caggttcatt tgaatacaac ttaacgaaac gacctgattc atatttccgc cgggcgagga
     2041 accggggtta taatcggaag ccagtgccag tgcgcatccg gcgcgaatca ggtcccgggc
     2101 ggggggcagc cgcatcccga ggaaataagc ggcggtgggt aataaagtgc aaaagccttt
     2161 ccagcccgaa cgggagaggc tttccatatc ttcttctgaa atgatttcca aatgatccac
     2221 ggaaaccgca ttcattttta tgccggtctg caggcccccg atgctgtgga gctggttgct
     2281 gtgcagcttg ggttgcagcc cgtaccggag gccggcccgt aaaatggttt cggtttcttc
     2341 cggagaaaaa aaaccatttt cacaaaacac atcaatataa tctgccagtt tctcccgggc
     2401 gatcaccggg agcatttcat ctatgatcag ccggagatat ccggggtgat cctcccggta
     2461 agcggccggg taggtatgtg cgcccagaaa gctggaccgg acggacaggg aagatttttg
     2521 tttcagtttt ctgatcaccc gcaggatttt cagttccgat tctaccgtca atccataacc
     2581 gctttttatt tcgatggcgc ccgtgcccag gcggctcact tcttccaggc gtttccagga
     2641 ttcattaaag agttcctctt cggatgcttc gcggacccgc tgaaccgtat gaagaatgcc
     2701 tccgccccgg gcatggatct cggaatacga aagcccgcgg atacggtcca cgaattcatt
     2761 ttcgcggctg ccggtaaaga cgaggtgcgt gtgactgtcg caccagcagg ggaataccag
     2821 tctgccgctg caatccagtt tatctgccgg catgcggaaa gaaggcttcc aggaagacat
     2881 cgttccgcag gcggcaattt ctccgtcttc gataatcagg taggcatctt ccaggtccgg
     2941 catcccggcc agttcacttc cgtacagcgg ttttgtgtct gcgcgaacat tcagcagttg
     3001 tttaatatga gtcagaatcc gggtgggcat tcaggttgaa tcagagattg aatttatttt
     3061 ttgtttcccg tgccgtttca tagcctgcat cggcatgacg gatcactccg agtcccggat
     3121 cgttcctcag gacccggctc aggcgcgcgg ctgcggcatc tgttccatcg gcaacaatca
     3181 ccattcccgc atggatgcta tatcccatac ccacgccccc gccgtggtgc aggctgaccc
     3241 aactggcccc gccggccgta ttgaccagtg cattcaaaat gggccagtcg gctacggcat
     3301 cacttccatc cagcatcgct tcggtttccc ggttggggct ggccaccgag ccggtgtcca
     3361 ggtgatcgcg tccgatcacc accggggcct ttaatttttt actcctg
//
LOCUS       DASYCU010000175         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4100477, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000175 DASYCU010000000
VERSION     DASYCU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4100477"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VGM24_03565"
     CDS             <1..428
                     /locus_tag="VGM24_03565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY4154470.1"
                     /translation="IWFKKELNTEMLNGFAKNTMNDFIGIRFIEVGENYIRASMPVDQ
                     RTKQAYGLLHGGASATLAETVGSVASAMVIDPEQFICVGVEINANHLKSVREGTVTAT
                     ALPLHLGSSSHVWEIKILDAQDNLICASRLTVYVIRKKN"
     gene            complement(425..850)
                     /locus_tag="VGM24_03570"
     CDS             complement(425..850)
                     /locus_tag="VGM24_03570"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4154471.1"
                     /translation="MKEPASRIIRAAGGLIANEQGNILFIFRRNKWDLPKGKMDGGET
                     PEECALREVEEETGIHEIELGEFLLITHHTYAENGLGILKETHWFRMKASGAARLIPQ
                     LEEQITEIRWIAPGEFDLVKQNTFPNILEVLHAAGYPVL"
     gene            925..1563
                     /gene="pyrE"
                     /locus_tag="VGM24_03575"
     CDS             925..1563
                     /gene="pyrE"
                     /locus_tag="VGM24_03575"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY4154472.1"
                     /translation="MTNEKAVAEKLLQIQAIKLNPDKPFTWASGWKSPIYCDNRTVLS
                     YPHHREFIKSEMCNLIFEEFESAEGLAGVATAGIAWGAMAADQLKLPFIYVRAKPKEH
                     GMGNQIEGHFEPGEKIVLIEDLISTGKSSLQVAEALRQSGLEVTGLISIFNYGFDQAK
                     NAFKSAGIPLRSLTDYATLIALAEEKGLVTAAQQKTLLSWRQDPASWGQEKS"
     gene            1631..2002
                     /locus_tag="VGM24_03580"
     CDS             1631..2002
                     /locus_tag="VGM24_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY4154473.1"
                     /translation="MKKLLFLSVLCSCFALLSRAQVVRKGAIETATIKVPTVQCDECK
                     DRIEKYVSREEGVQKITVDYKRKTAKVTYLWDRTNVENVKAAIANAGYDADDVTANTE
                     AYNKLPTCCKKPDDGGGHEKK"
     gene            complement(2007..>2642)
                     /locus_tag="VGM24_03585"
     CDS             complement(2007..>2642)
                     /locus_tag="VGM24_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154474.1"
                     /translation="MKKIPVCIGLVILFHGPAFSQKFPGEVDSSFLPVLQMHYQAEYT
                     FDSPLDNEAWQNQQGGLHVSFGSTDRAYFRTEVPEKKETLEYEGVGWRGERLNTKLLI
                     WFPDTLQQVRITVNDLVSADGKHISGKNISLNLVRYVLANYPYGSKEKIRILKELAGR
                     SPNKNIQAHWQAFEKYLGVFPAEHEFNEEKITAEVNKGKALLDELSDLMSQ"
BASE COUNT          685 a          664 c          633 g          660 t
ORIGIN      
        1 cgatctggtt taaaaaagaa ctgaataccg aaatgctgaa tggttttgca aaaaatacca
       61 tgaacgattt tatcggcatc cggttcatag aagtgggtga aaattatatc cgggccagca
      121 tgcccgtaga ccagcggacg aaacaggcct atggattatt gcatggaggt gcttcggcca
      181 cgctggcgga aacggtgggc agcgtagctt ccgccatggt gatagatccg gagcagttta
      241 tctgtgtggg cgtcgagatc aatgccaacc acctgaaaag cgtgcgggaa ggaaccgtaa
      301 cggccacagc ccttcccctg cacctgggat cgtcttccca tgtctgggag atcaagatcc
      361 tggacgcgca ggataacctg atttgcgcaa gccggctgac ggtttatgta attcgtaaaa
      421 agaattagag aacgggataa cccgcggcat ggaggacctc caggatattg ggaaaggtat
      481 tctgttttac caggtcaaat tcacccggcg cgatccacct gatctcggtg atctgttctt
      541 ccagctgcgg aatcaaccgg gccgctccgg acgctttcat gcggaaccaa tgggtctctt
      601 ttaaaatgcc cagtccgttt tctgcgtacg tatgatgagt tatcagcaaa aattcaccca
      661 actcaatttc gtggatgccg gtctcttctt ccacttcgcg caaggcgcat tcttccggcg
      721 tctctccccc atccattttc ccttttggca gatcccattt attccggcgg aatatgaaca
      781 ggatatttcc ctgttcattg gcgatcagtc cgcccgccgc gcgtatgatc cggcttgctg
      841 gttctttcat tttcatcccc ttcaaagata gatccatcaa ggtaaaggaa gcggaaaccg
      901 catagaattt tttatcttca cgccatgacc aacgaaaaag cagttgccga aaaactctta
      961 cagattcagg ccattaagct caatccggac aagcccttca cctgggcatc cggatggaag
     1021 agcccgatct attgtgataa ccggaccgtg ctttcttatc cccatcaccg ggaattcatc
     1081 aaatccgaaa tgtgcaacct gatctttgaa gaatttgaat ccgcagaagg acttgccggt
     1141 gtggccacgg caggcatcgc ctggggcgcc atggcggccg atcagctcaa acttcctttt
     1201 atttacgtgc gcgccaaacc gaaagaacac ggcatgggga accagatcga aggacatttt
     1261 gaaccggggg aaaaaatcgt gctgatcgaa gacctgatct ccacgggcaa gagcagcctt
     1321 caggtggcgg aggcgctccg gcagtccgga ctggaggtca ccggcctgat ctccattttc
     1381 aattacgggt ttgaccaggc aaagaatgcc tttaaatcgg caggtattcc gctccggtcc
     1441 ctgacggatt atgccacgct catcgccctg gcagaagaaa aaggcctggt cacggcagcc
     1501 cagcagaaga ccttattgag ctggcggcag gatcccgctt cctgggggca ggaaaaaagc
     1561 tgaaaagtta aaactggaaa gagtcctgat attccttttg attttttaac ttttacttta
     1621 aatttatacc atgaaaaaat tactctttct ctccgtttta tgcagctgtt ttgcgctgct
     1681 gtccagggca caggtggtgc ggaaaggcgc cattgaaacc gcaaccatta aagttcccac
     1741 cgtgcagtgt gatgaatgta aggaccggat cgaaaaatat gtcagccggg aggaaggggt
     1801 tcagaaaatt actgtggatt ataaaaggaa aacggcgaaa gtcacttatc tctgggaccg
     1861 caccaatgtt gaaaatgtaa aagcggcgat cgccaatgcc ggatacgacg ccgatgatgt
     1921 taccgcgaac accgaagctt ataataaatt acccacctgc tgtaagaaac ccgatgatgg
     1981 cggagggcat gagaagaaat gatttgttat tgactcatca gatcgcttag ttcgtccaag
     2041 agggcttttc ccttatttac ttcagccgta attttctctt cgttgaattc atgttcagcc
     2101 gggaacacgc ccaggtattt ttcaaaggcc tgccaatgcg cctgaatatt tttattgggg
     2161 gatctgcccg ccagctcttt taagatcctg atcttttctt ttgatccata aggataattg
     2221 gcaaggacat aacgaaccag attcaggcta atgttcttcc cgcttatgtg cttcccgtca
     2281 gcgctcacca gatcattcac ggtgatcctc acctgctgta aggtatccgg aaaccatatc
     2341 aataacttgg tgttcaaccg ttcgcccctc cagcccaccc cttcatattc cagcgtttct
     2401 tttttttcag gaacttctgt tctgaaatag gctctgtccg tcgacccgaa tgaaacatgc
     2461 aatccgccct gctgattttg ccatgcctca ttatcaagcg gggaatcaaa agtatattcg
     2521 gcctgatagt gcatttgaag aacaggaaga aaggatgaat ccacttcgcc cggaaacttc
     2581 tgcgaaaaag cggggccgtg aaaaagaatc acaagcccaa tacataccgg aatttttttc
     2641 at
//
LOCUS       DASYCU010000176         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_767156, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000176 DASYCU010000000
VERSION     DASYCU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767156"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VGM24_03590"
     CDS             complement(<1..526)
                     /locus_tag="VGM24_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154475.1"
                     /translation="MNSILKKILDSNKQMEKAEANATTRELHDPATGNNRRNFLKKTA
                     RGGISLGGLMCLPFEDLVAQTTSTVSRASSPSDLKITDMRYALTTVLGGTAIIRIDTN
                     QGIYGLGEVRDGADPRYALMLKSRILGQNPCNVEMIFKSIKQFGGPGRQAGGVCGVEI
                     ALWDLCGKAYNVPAW"
     gene            complement(576..1508)
                     /locus_tag="VGM24_03595"
     CDS             complement(576..1508)
                     /locus_tag="VGM24_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY4154476.1"
                     /translation="MSKKSLLAIAILSGSYFAQAQRVGSSPEYIKTLTADWKGERLPD
                     GRPKVSDAILERLKKVSMEDAWGVLRNKGYQNQYEGDWIVIQPDSVMTGRVVTAQYMP
                     SRPDLIKVVKEEGKAEDRDQQGGTNSWPIDILTSGDVYVADGYGKMADGTLIGDNLGN
                     SIYAKSQRGVIFYGSVRDQAGLSEIKGFNGWIKGQDPSYIQQMMLTSINAPIRIGRAV
                     VLPGDVVLANRYGTIFIPAALVDELVITSEITGLRDEFGHQRLREKKYLAGQIDSQWS
                     EEIRKDFLNWLKSYPGKLPMTKAELDDYLKSRNY"
     gene            1726..2439
                     /locus_tag="VGM24_03600"
     CDS             1726..2439
                     /locus_tag="VGM24_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY4154477.1"
                     /translation="MPTAPIKYLILDDDEMDRLSIEIEAAKFPFLQKIASCSHPVEAF
                     EMITRFQPGVVFLDIEMPDMSGMDLIKTKGISTALPVLITSHPEFALESYELDAFDYL
                     LKPLSAERFARCARRLQDFFEMRMKAFDFDENQESGSIVIKQGHDKFKLPIREILYLE
                     AMKDYTRIKTASGQYLVLTTLSGILNKLPSDLFVRIHRSYVVNRGKIDSVEKNMIYIQ
                     SQILPIGKLYKNALKPFFS"
     gene            2493..>3841
                     /locus_tag="VGM24_03605"
     CDS             2493..>3841
                     /locus_tag="VGM24_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154478.1"
                     /translation="MRSSAVFLSGLLLLALDVRAQEPDLSRIKSDQEKVKVWMDYCDS
                     LRINQQGIKGNYILLQQAALKGLKMPGAQNSEARSRLYFYTALGYYYQVKFDSAQTYF
                     YQSLHEAQKGHIARQIAKACVALIPVNFQLQQAARVEECRNILQSIVDTTQNISILQD
                     GYYALGSYYQQKSYYSTAQDYFIKSLESREKEVDTTRDQKKKFDYAIQCDLLSKLYLN
                     TQMVDKSLAVLRQGERFAGVSPTVGNRLLSSFVEAFTTSGKIDSALYYDGRLEEQTKD
                     NPIFPSEIVSSDLNIAIYYIDHHQYSRALPYLNRGDTLSARVKSPILLFQSQLITGRY
                     DVETGKFEEAISLLNQSLPVAKQYSRELYSNALKSMALAQKGKGNLKAALSYYEQYAQ
                     QADSITKEKLSTNFADQETRYQTNRKEERIASLDKENRLEVLELQNASRTRLLLVIGL
                     "
BASE COUNT          956 a          990 c          876 g         1019 t
ORIGIN      
        1 gccatgccgg aacgttgtag gcctttccgc atagatccca aagggcgatt tccacgccgc
       61 atacaccacc cgcctggcgg cccggacctc caaactgttt gatcgattta aaaatcattt
      121 ccacgttaca gggattctgt cccaggatgc ggctcttcag catcagggcg taccgggggt
      181 cggctccgtc ccgcacttcg cccagtccgt aaatcccctg gttcgtatca atccggatga
      241 tggccgttcc acccagcacg gtggtcagcg cataacgcat gtcggtaatc ttcaaatccg
      301 acggacttga agcccggctc acagtagacg tggtttgcgc gaccaggtct tcaaagggaa
      361 ggcacatcag gccgcccagg gaaattcccc cacgggccgt ttttttgagg aagttgcgcc
      421 ggttatttcc ggtggccgga tcgtggagct cccgggtagt ggcgtttgcc tccgcctttt
      481 ccatttgctt attgctatcc agaatttttt tcagtatgct attcatgttc ttgcattttt
      541 aatcgtttta agaattagtt catccccggt gcttatcaat agttacgcga cttcaggtaa
      601 tcatccagtt ccgctttggt catcggtaat ttacccggat agcttttcaa ccagttcaga
      661 aaatctttcc tgatttcttc agaccactgg ctatcgatct ggccggccag atattttttc
      721 tcccgcagcc gctgatggcc gaattcgtcc cgcaagccgg tgatttccga agtgatcacc
      781 agttcatcga ccaaagcagc cggaataaaa attgttccgt acctgttggc cagaaccaca
      841 tcgcccggca atacaacggc cctgccaatg cgtatcggcg cattaatgga agtaagcatc
      901 atttgctgga tataagaggg atcctgtcct tttatccatc cgttgaaccc cttgatttcc
      961 gagaggccgg cctgatctct taccgatcca taaaaaataa cccctctttg ggatttggcg
     1021 taaatggaat ttcccagatt atctccgatc agcgttccat cggccatttt tccatagccg
     1081 tccgccacat acacatcccc ggaagttaaa atgtcaatcg gccaggaatt tgttcctccc
     1141 tgctggtcgc ggtcttccgc ctttccttcc tccttcacca ctttgatcag atccggcctg
     1201 gagggcatgt actgggccgt tacgaccctt ccggtcatga cactatccgg ctgaataacg
     1261 atccagtctc cttcgtactg attctggtat cctttgttgc gcaatacgcc ccaggcgtct
     1321 tccatggaaa ctttttttaa tctctccagg atggcgtccg aaactttcgg tcgcccgtcg
     1381 ggtaaacgtt cgcctttcca gtccgccgtg agggtcttaa tatattctgg tgacgaaccg
     1441 acgcgttggg cctgcgcaaa ataactgccc gataaaatgg caatggccaa taaagatttc
     1501 ttactcataa cgatcatccg gtttttttaa aaataggata taagttattc attaaatatt
     1561 atctgtcgaa aataatttca cctgaaacct gtttttcctc tccggcgggt atgaccggaa
     1621 ggatattttc caattctgcc ggataagcgg aaattatgat tccggacgtt tttttttatg
     1681 tagattagca gccgtttata tatctaattc acctcacctg tactcatgcc gaccgcaccg
     1741 ataaaatacc ttatcctgga tgatgatgag atggatcgcc tcagcatcga aattgaagcg
     1801 gccaagtttc cctttcttca gaaaatcgcg tcctgttccc atcccgtgga agcttttgaa
     1861 atgatcaccc gttttcaacc gggcgttgtc tttctggata tcgaaatgcc ggatatgagc
     1921 gggatggacc tgatcaaaac aaaaggcatc tccacggcct tgccggtgct gatcacttcg
     1981 catccggagt ttgccctgga gagttatgaa ctcgatgcct ttgactattt attaaagccg
     2041 ctgtctgccg aacggtttgc ccggtgcgcc cgccgcctcc aggatttttt cgaaatgcgc
     2101 atgaaggcct ttgatttcga tgaaaaccag gaatccggaa gcatcgtcat caaacagggg
     2161 catgataaat ttaaattacc catccgcgag atcctgtatc tggaagccat gaaggactat
     2221 acccggatta aaacggcctc cggccagtat ttggtgctga ccactttaag tggtatattg
     2281 aataaacttc catcggatct gttcgtacgc attcaccgca gttatgtagt gaatcgcggg
     2341 aaaatcgatt cggtggaaaa gaacatgatc tatattcaat cccagattct ccctatcgga
     2401 aaattatata agaatgcatt gaagcctttt ttcagctgat ccgttttcaa tatcccggtg
     2461 atacatgatc caaaacgtta acgtggcctg gtatgcgcag ttctgctgtt tttctttccg
     2521 gtttactatt gcttgcttta gacgtcaggg cgcaggagcc cgacctgtcc cggatcaaat
     2581 ccgatcagga aaaggtaaag gtctggatgg attattgcga ttcgctgaga ataaaccagc
     2641 aggggataaa gggtaattat atcctcttgc aacaggcggc gttaaaggga ttgaagatgc
     2701 ccggcgcaca gaattccgaa gctcgttccc gcttatattt ctataccgca ctgggatatt
     2761 attaccaggt gaaatttgat agtgcccaga cctatttcta tcaaagcctg cacgaagcgc
     2821 agaaagggca tatcgcccgg caaattgcga aagcctgtgt ggcgctgatc ccggttaatt
     2881 tccagctgca gcaggccgcc agggtggagg aatgcaggaa tattttgcaa tcaatcgttg
     2941 ataccacaca aaatatttcc attttacagg acggatatta tgccctgggc agctattatc
     3001 agcaaaaatc atattacagc acggcgcagg attattttat caaaagcctg gaatcgcggg
     3061 aaaaggaagt ggataccacc cgggaccaga agaagaagtt cgactatgcg attcagtgtg
     3121 atctgctttc caaactgtat ctcaatacgc aaatggtgga taaaagcctg gccgtactga
     3181 ggcagggaga acgttttgcc ggcgtttcgc ccaccgtcgg aaaccggttg ctcagctctt
     3241 tcgtagaggc atttacaacc tccggcaaaa tcgattcagc gctgtattat gatggccggc
     3301 tcgaagagca gacaaaggat aaccccattt ttccctctga gatcgtttct tccgatctga
     3361 acatcgccat atattatata gatcatcacc agtatagccg ggcgctgcct tatttgaacc
     3421 ggggcgacac gctgtctgcg cgcgttaaat cgcctattct cctgttccag agtcaactca
     3481 taaccggccg ttacgacgta gagaccggga aatttgaaga agccatttcc cttttgaatc
     3541 agtcgcttcc ggtcgccaag caatacagca gggaacttta ttccaacgcg cttaaatcaa
     3601 tggcattggc ccagaaagga aagggaaacc tcaaagccgc cttatcctat tacgaacaat
     3661 atgcccaaca ggcggattcc attaccaaag aaaaattatc caccaatttt gccgaccagg
     3721 aaacacggta ccaaaccaac cggaaggaag aacggattgc ttccctggac aaggaaaacc
     3781 gcctggaagt gctggaattg cagaatgcct cgcgaacccg gctattgctc gttatcggac
     3841 t
//
LOCUS       DASYCU010000177         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2216066, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000177 DASYCU010000000
VERSION     DASYCU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216066"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..3365
                     /locus_tag="VGM24_03610"
     CDS             333..3365
                     /locus_tag="VGM24_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4154479.1"
                     /translation="MKRLLFPFLLVFFFSANAQQTLYLNDPQAAYKQAQEYYQKQNYS
                     LAYPIFKELEQDQQNKLQVRESFEYQNVHFYTLVCSLNQDDSASVAPAKQFIERENNA
                     ARAQMLSYHLAEYEFRRGNYETALHLYETAGIDNLSNAEIANEKFHQGYAYFVKKDFD
                     RARPLMDAIRQLPRDPNYIDANYYYGYISFYQKKYSDAAAAFAIVEKDPKYSSIVPYY
                     IANIYLIQKQKDKAIEYASAKLASGNQYYDAELRELTGHGYYEQKDFAKALPYLEAFV
                     GKSKKVSRQDMFELSYCYYQLKNYKKAIEGFKQLGGKEDSLAQNAMYLLGDSYLKTGE
                     KANARNAFLFCASNSSYPVQKEISQFHYGKLSYELGYPDAALTSLRGFITEYPKSTYV
                     PEAKELLLSVLANTSNYKEALGLMDSLTNPSANARQLHARILYGRATELINDGMLQNA
                     SDLLDKALKVPDNTAVLPFINFWKGEIAYRSNRLDEAIPFFYEYLKMPVSRGEVNPAN
                     AKYTLGYCYLRKENYREAQGFFEQVVRNPRIDASPLEQDAYIRDADCYYMSRDFKTAV
                     NMYNKVLDYSWPSSDYATYQKAMVAGVNSSSEKISLLNGLIRKFPGSSLVPEANMEIA
                     NTYLSNENFRESLTYLKNVLNSSGNDALKPRAYLRSGIAYYNLENNKEALNQYNQLLK
                     LYPNSQEAQEALDNAKSIYVAEGRSDEYINFAKSMGVSISTSQEDQLAYQEAEVQFNN
                     GNFALAEQKFEDYLKKFPDGKYFLEANYYKSEIYASQKNWPQAVTGYEMVADKAPNKF
                     AEKSLLQAARLNFFNLKDYAKSETYFTKLKDFASSQENKMEAMRGLLRSQYELQHWSA
                     AMDNARDLLDQKGIGSDDKIIANMIIGKSYLTDGKCDQALPYFKTVVSLSRATYGAEA
                     QYQIAECELNGNRLKEAEKSAFEVIHKSGSYEYWVTKSYLLLGDIYLKEKDYFNAKAT
                     FQSLVENAKIEELKVQAEQRLDQVKQEEEKNSKISNDN"
     gene            3389..>4243
                     /locus_tag="VGM24_03615"
     CDS             3389..>4243
                     /locus_tag="VGM24_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154480.1"
                     /translation="MKGFICLVTVLLMAAVSLKAQDTTKRKTINITSTFKPTLRNNAK
                     INFNAEAPVIDTAHPQLNYNLPVQYLPLQYQPVGLSPLALPGDTMKIWNNDNYIKVGV
                     GNVHLPYVKAGLSFGDKKYSFFNVFAEGYSSKGDLKYQKSSLFDAAITGTVKTPHNLE
                     WTGKLGFRGDGYYLYGYQPHDLQFANSQLQQQFLTFEGKIGLRNSVPTEFGLTYNPNL
                     KVVAFGDNQTPRGSEINTVVNLPLQKTIGEHFGINLGVTADLTHYAVNDVFTLNNNLF
                     YVSPAVVYH"
BASE COUNT         1198 a         1042 c         1039 g          964 t
ORIGIN      
        1 gggaattgcg gtgaccgatc tggaagggat cataaaaggt tctcgacagg gagctgaaat
       61 tcgaaatttt ggggagcccg tgtgccatca ggaggaggag ccccgtcacc acccgttgca
      121 gcagcatggc aaaattaaat gcattatcgg aataggtggt ggatatcagt tttttcatcc
      181 ttcaatattt agccaaatgt aatggatttg cccttttagc gattgttcta aagaaaatgt
      241 taatcctttc agttatccac acctgtttgg aacaatcttt ggtattagaa gaaaatattc
      301 agaaacttga gcgcgtgaat aacactatac ggatgaaacg attactcttt ccgttcctcc
      361 tcgttttctt tttttcggcc aatgcgcaac agaccctgta cctgaatgat ccccaggcgg
      421 catacaaaca ggcgcaggaa tattatcaga aacagaatta cagtctggct tatccgatct
      481 tcaaagaact cgaacaggat cagcaaaata aattgcaggt cagggaatca ttcgaatacc
      541 agaatgtgca tttttatacg ctggtctgtt ctctcaacca ggacgacagc gcttcggtgg
      601 ctccggcaaa gcagttcatc gaaagagaga ataatgccgc gcgggcgcag atgctgagtt
      661 atcatctggc ggaatatgaa ttccgcaggg ggaattatga aaccgccctg catctttatg
      721 aaacggcggg gatcgacaat ttaagcaatg cggaaatcgc caacgaaaag ttccaccagg
      781 gctatgccta ttttgtgaag aaggatttcg accgggcgag acccctgatg gatgccatcc
      841 gccagcttcc ccgcgatccg aattatattg acgccaatta ttactacggc tatatctcct
      901 tttatcagaa aaagtacagt gatgcggccg ccgcctttgc tattgtggag aaggatccga
      961 aatacagcag catcgttccc tattatattg cgaatatcta cctcattcaa aagcagaagg
     1021 acaaggccat agaatatgcc tctgccaagc tcgcttccgg caaccagtat tacgatgcgg
     1081 aattgcggga actgacgggc cacggatatt atgaacagaa ggactttgcg aaagcgcttc
     1141 cctacctgga agcgtttgtg ggcaagtcga agaaagtatc ccgccaggat atgtttgaac
     1201 tgtcctactg ttattaccag ttgaagaatt ataaaaaagc gatcgaagga ttcaaacaac
     1261 tcggcggcaa ggaagattcc cttgcgcaga acgcgatgta cctgctggga gacagttacc
     1321 tgaaaaccgg ggagaaagca aatgccagaa acgcctttct cttctgcgct tccaacagca
     1381 gctatcccgt tcaaaaagaa atttcccagt ttcactacgg caaactttcc tatgaactgg
     1441 gttatcccga tgcggcgctg accagcctcc ggggctttat cacggaatat ccgaaatcca
     1501 cttatgtgcc ggaggcaaaa gaattattac tgagcgtgct ggccaatacg agcaattata
     1561 aggaggccct gggcctgatg gacagcctga ccaatccctc cgcaaacgcg cggcagctgc
     1621 atgcccggat actctatggc cgggccacgg aactgatcaa tgacggcatg ctgcagaatg
     1681 caagcgatct gctcgacaag gcactcaaag ttccggacaa cacggcggtc cttcccttca
     1741 ttaatttctg gaagggtgaa atagcttacc ggtccaaccg cctcgacgaa gcgatccctt
     1801 tcttttatga atacctgaaa atgccggtga gcaggggcga agtgaacccg gcaaatgcaa
     1861 aatacacgct tggatattgt tacctgagaa aagagaacta ccgggaagca cagggttttt
     1921 ttgaacaggt ggtcaggaac ccccgcattg acgcttctcc cctggaacag gatgcctata
     1981 tccgcgatgc ggattgttat tatatgagcc gggactttaa aacggcggtg aacatgtata
     2041 ataaggtgct ggattattcc tggccttcct ccgactatgc cacgtatcaa aaagccatgg
     2101 tggccggtgt gaattcctcc tccgaaaaga tctccctgct caacggcctg atccggaaat
     2161 tccccggctc cagcctggtg ccggaagcaa atatggagat cgccaatacc tatctttcaa
     2221 acgaaaattt ccgcgaatcc ctgacctacc tgaaaaacgt cttgaactcc agcgggaacg
     2281 atgcgctgaa acccagggcc tacctgcgat ccggcattgc ctactataac ctggaaaata
     2341 ataaagaggc cctgaatcag tacaaccagt tgctgaaact gtatcccaat tcgcaggaag
     2401 cacaggaagc gctcgataac gcgaaatcca tttatgtggc ggaaggccgg agcgacgagt
     2461 acatcaattt tgcaaaatcc atgggcgttt ccatatccac cagccaggaa gaccaactgg
     2521 cctaccagga agccgaagtg caattcaaca acgggaattt tgccctggct gaacagaaat
     2581 ttgaagatta cttaaagaaa tttcccgacg gcaaatattt cctcgaggcc aattattata
     2641 aaagcgagat atatgccagc cagaaaaact ggccccaggc cgtaaccgga tatgaaatgg
     2701 tggcggataa agcgcccaat aaatttgcgg agaaatccct gttgcaggcg gcccggctga
     2761 attttttcaa cctgaaggat tatgccaaat cggagaccta ttttaccaag ctgaaagact
     2821 ttgccagctc ccaggaaaat aagatggaag ccatgcgcgg cctgcttcgc agccagtacg
     2881 aattgcagca ctggtccgcg gccatggaca atgccaggga cctgctggat cagaaaggca
     2941 ttgggtccga cgataagatc attgccaata tgatcatcgg gaaatcctat ctgacggacg
     3001 ggaaatgcga tcaggcgctg ccttatttta aaaccgtcgt ttccctcagc cgcgccacgt
     3061 acggggcgga agcccaatac cagatagcag aatgcgagct gaacggcaac cgcctgaaag
     3121 aagcggagaa atcggcgttt gaagtgatcc acaagtcggg ctcctatgag tattgggtca
     3181 ccaaatccta tctgctgctg ggggatatct atctgaaaga aaaagattat ttcaatgcca
     3241 aagccacttt ccagagcctg gtggaaaatg cgaagattga agaattgaaa gtgcaggccg
     3301 agcagcgcct cgaccaggtg aagcaggaag aagagaagaa tagcaagatc agcaatgaca
     3361 attgagtgct cctaatcatt tgaactttat gaagggattt atttgtttag ttaccgtgct
     3421 gctgatggcc gccgtttcct taaaggcaca ggatacgacg aaacggaaga ccatcaacat
     3481 cacttccact ttcaagccca cgctgagaaa taacgcaaag attaatttca atgcggaggc
     3541 gccggtgatc gataccgcgc atccccagct gaattataat ctgcccgtcc agtacctgcc
     3601 gctgcagtat cagccggtag gattaagtcc ccttgcgctt ccgggggata ccatgaagat
     3661 ctggaacaac gataattata tcaaggtggg cgtggggaat gttcacctgc cttatgtgaa
     3721 ggccggcttg agcttcgggg acaagaaata cagtttcttt aatgttttcg ccgaagggta
     3781 ttcatccaaa ggcgatctga aataccagaa gagcagcctt tttgatgccg ccatcaccgg
     3841 aacggtcaag accccgcaca acctggaatg gaccggaaag ctcggtttcc ggggggacgg
     3901 ttattacctt tacggatacc agccccacga cctgcagttt gcgaacagcc agctgcagca
     3961 gcagttcctg acctttgaag gaaagatcgg tttacgcaac agcgtgccga cggaattcgg
     4021 tctcacctat aatccgaacc tgaaagtggt tgccttcggc gataaccaga ctccccgcgg
     4081 ctcggaaatc aacaccgtgg tgaacctgcc tttacaaaag accatcggcg aacattttgg
     4141 aataaacctg ggggtgaccg ccgacctgac ccattacgcg gtgaatgatg tttttacctt
     4201 aaacaataat ctgttttatg tttcgccggc cgtggtttac cac
//
LOCUS       DASYCU010000178         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3377470, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000178 DASYCU010000000
VERSION     DASYCU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377470"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VGM24_03620"
     CDS             complement(<1..563)
                     /locus_tag="VGM24_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154481.1"
                     /translation="MKGSSSASRRRFLQQMSGTALAMAAANSSLAFADQWQNRVDSYS
                     RKISANDKIRIGVIGIGIQGNNDLEAALKVPGTEMVAACDLYKGRLERAKEVYGKDLF
                     VTADYRELLQRKDVDAVIIATSDHWHSRISIEALNSGKAVYCEKPMVHKISEGLGVIE
                     AWKKNRKTMQVGSQRVSNIGTLKAREA"
     gene            complement(654..1085)
                     /gene="ssb"
                     /locus_tag="VGM24_03625"
     CDS             complement(654..1085)
                     /gene="ssb"
                     /locus_tag="VGM24_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY4154482.1"
                     /translation="MIKLQVIGNLGKDCVTNLVNGKNVINFNVAHTEKFRDAQGNQKE
                     KTVWVDCAYWTDRTAIAPYLKKGTQVYAEGTPDLRTYQKTDGTQGVSLTLRVQSVQLL
                     GSRNTNDSAGYGNDIQNQASSSMSGIPSPGEISAPVDDLPF"
     gene            1166..2293
                     /gene="mnmA"
                     /locus_tag="VGM24_03630"
     CDS             1166..2293
                     /gene="mnmA"
                     /locus_tag="VGM24_03630"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY4154483.1"
                     /translation="MGISIFAPMSRKGKVLVAMSGGIDSTITALMLHDQGYEVIGITM
                     KTWDYATSGSSKKETGCCNLDSFNDARMAAVQHGFPHFVLDIRDEFGTAVIDNFVEEY
                     LAGRTPNPCVMCNTHIKWRALLKRADALNCDFIATGHYANIHQHPNGRYFLSKGIDEL
                     KDQSYVLWGLQQDLLSRTLLPLGNYRKTEIRQMALDYGYPELAKKSESYEICFVPDND
                     YRGFLKKKMPALEATVEGGSFVDVHGKIIGKHRGYPFYTIGQRKGLDIALGRPVYVTA
                     IHPENNTVVLGEETDLAGQSLMVGGLNLMKYDSVPDGMEALTKIRYKDKGSRSNLFPE
                     DNQVRVQFYEPVKSIAPGQSAVFYEGDDVIGGGIIRRTVNA"
     gene            2373..2849
                     /locus_tag="VGM24_03635"
     CDS             2373..2849
                     /locus_tag="VGM24_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY4154484.1"
                     /translation="MSKSHQYNTRLRWTGNTGKGTENYRAYERAYTVSINGKPEMAGS
                     SDPAFRGDPAKYNPEEMLVAALSACHMLSFLHFCSASGVVVVDYSDAAIGAMEETNDG
                     NGRFTEVILRPNVVVREVSMTEKVNALHDKAHQYCFIANSCNFPVLHQPTCSVVDI"
BASE COUNT          738 a          718 c          703 g          731 t
ORIGIN      
        1 aacgcctcgc gggctttaag ggttccgata ttgctcacgc gctggcttcc cacctgcatg
       61 gtcttcctgt tttttttcca ggcctctatc accccaagcc cttcactgat cttatgcacc
      121 atgggcttct cacaataaac cgccttgccg ctgttcaatg cttcaatgct gatccgggaa
      181 tgccagtgat cgctggtcgc gatgatcacc gcatcaacgt ctttcctttg caacagttcc
      241 cggtaatctg ccgttacaaa aagatccttc ccataaactt ccttcgcccg ctccagtctc
      301 cctttataca aatcgcatgc ggcaaccatt tcggtgcccg gcactttcag ggccgcttcc
      361 aggtcattat tgccctgaat gccaatgccg atgacgccaa tgcggatttt atcattcgcc
      421 gaaattttcc tggaataact gtctacccgg ttttgccatt ggtcggcaaa tgccagggaa
      481 gaattggcag cggccattgc caacgccgtg ccactcatct gttgcagaaa acgccggcgg
      541 gaagcggaag aagatccttt catgttggtt cgttttaagt atatgaaaga ggaaggatag
      601 gatggacaaa aagtgccctc acacatcatg agagcacttt tttatcggaa tttctaaaag
      661 ggaagatcgt cgaccggggc agagatctca cccggagaag gaatgcctga catacttgag
      721 gaagcctggt tttggatatc gtttccgtaa ccagcactat cattcgtatt cctgctgccg
      781 agcaattgta cactctgtac acgcagcgtt aaggatacgc cctgcgttcc gtctgttttc
      841 tggtaggtgc gcaaatcggg agtgccttct gcatacacct gtgttccttt tttcagataa
      901 ggagcgatcg ccgtccgatc ggtccaatac gcacaatcca cccacactgt tttctccttc
      961 tggtttccct gggcatcgcg gaatttttcc gtgtgtgcca cattgaaatt gatcacattt
     1021 ttaccgttca cgagattggt cacacaatct tttccaagat ttccgatgac ttgcaatttg
     1081 atcataactt aaaatttagc tgcgtactta aaattgtttg aaaaggcaat ttacaggatt
     1141 taggaagccg ataaaatact attttgtggg aataagtatc tttgcaccca tgagcagaaa
     1201 aggaaaagtg ctggtagcga tgagcggagg tattgacagt accattaccg ccctgatgct
     1261 ccatgaccag ggttatgagg taattggaat taccatgaag acctgggatt acgccacgtc
     1321 gggatcttcc aaaaaagaga ccggctgttg caacctggac agcttcaatg atgcccgcat
     1381 ggcggccgtt cagcacgggt ttccgcattt cgtgctggat atccgggatg aattcggaac
     1441 tgccgtgatc gacaattttg tggaagaata ccttgccggc cgcaccccca atccctgcgt
     1501 gatgtgcaat acccacatta aatggagggc tctgttaaaa agagctgacg ccctgaattg
     1561 cgactttatc gccaccggtc attatgcgaa tattcatcag cacccgaacg gccggtattt
     1621 cctcagcaag ggcatcgacg aactaaagga tcagagttat gtactctggg gcctgcagca
     1681 ggacctgctc agccgcaccc tgcttccgct cggcaattac cgcaaaacgg aaatccggca
     1741 gatggccctg gattatggct atcccgaact ggcaaagaaa agcgaaagct atgagatttg
     1801 ttttgtgccg gacaacgact accggggttt cctgaagaaa aaaatgcccg cgctggaagc
     1861 gactgttgaa ggaggaagtt tcgtggatgt tcatggaaag atcatcggca aacaccgcgg
     1921 ttatcctttt tataccatcg gtcagcggaa aggactggat attgccctgg gaagacccgt
     1981 ttatgttacg gccattcatc cggaaaataa tacggtggta ttgggagaag aaactgacct
     2041 ggccggacaa agcctgatgg tcggcggact caacctcatg aaatacgatt cggttcccga
     2101 tggcatggaa gccctgacaa aaatccggta caaggataaa ggaagccgga gtaatttatt
     2161 tcccgaagac aaccaggtcc gcgtgcagtt ttacgaaccg gttaaaagta ttgcccccgg
     2221 gcagagcgcc gttttttacg aaggagacga tgtaatcgga gggggaatta tccggagaac
     2281 ggtcaatgcc tgatggatgc cgtattgaat tgccccgatg catcattttg acagctaaaa
     2341 cgcactcact gcgaaaataa tcccctgtaa atatgtccaa atcccaccag tacaacaccc
     2401 gtctccgctg gaccggcaat accggaaagg gaacggagaa ttaccgggct tatgaaagag
     2461 cctataccgt ttcaataaat ggcaagccgg aaatggcggg ttcctcagat ccggcctttc
     2521 gcggtgatcc tgccaaatac aatcccgaag aaatgctggt tgccgcctta tccgcctgtc
     2581 acatgctttc ctttctgcat ttctgctcgg catcaggcgt ggtggttgtg gattattcag
     2641 atgcggccat tggtgcgatg gaagaaacaa atgatggaaa cggaagattt accgaggtaa
     2701 tcctgaggcc aaatgtggtc gtcagggagg tttccatgac cgaaaaagta aatgcgcttc
     2761 atgacaaagc gcatcagtat tgttttattg ccaactcctg taattttccc gtattgcatc
     2821 agcccacctg ttcagtggtg gatatataat tgatggtgga catcaattat tctccgcgta
     2881 aaccggaatc
//
LOCUS       DASYCU010000179         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1200671, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000179 DASYCU010000000
VERSION     DASYCU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200671"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="VGM24_03640"
     CDS             <1..1610
                     /locus_tag="VGM24_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY4154485.1"
                     /translation="VYQAGRTLVVWPYSDNVPSIMLMHRARWGEWIEQRMPAADRIKP
                     GEEIETATQFFRLNEGPIMPSMNHFQKFWKDVNVRLQDSTPDWGKDARIYEVEVGQKR
                     NPESSRTPYTSMAELASDLPRIQKMGFNIIELMPHMPFPSYSVHDYFNIEVQYGPEKE
                     LRAMIDQAHQLGVKVFLDIVLHGVTDKTLNPLAIFDKNPLVKEHPDWFSHNEYGEIAR
                     TYTWAFDHRNPEVQQYMVKVFNFYVDKLNPDGFRVDAVTWNCFPNWSENLNRPAYSSF
                     YGTASMMDLVRKETHKIKPELVFYNEAAGPLYFRAFDLSYNYDEQWLYAAILSGRRTG
                     QGPGQSRRGAGWTEPGMSAQDLGEWLEMRRRAMPKGLIRVHHVDSHDAYDGSGSRFRR
                     EILGIPATRMLFAMTSFIDGGVMTFKGAELGSEDYYSKVLSIRESIPALKSGTCEYLT
                     ITSENERVFTPLRRLGNEWALPVISFSREPIDTWISLAPLKLNRDSVYTLREAFSGME
                     KTGTGRDLASLNVKLDSLGVQVWTLKN"
     gene            1681..4422
                     /locus_tag="VGM24_03645"
     CDS             1681..4422
                     /locus_tag="VGM24_03645"
                     /inference="COORDINATES: protein motif:HMM:NF039643.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEY4154486.1"
                     /translation="MGIKKIISFIYRQEIRMMICLAACLITASPILNAQSGSASAALD
                     SATRFAYYPGLHKLEVRISDLPAEVLAGHQEATAVISGPGGKKTIARQVFSITGKGGS
                     CMISLPPLADGEYELMVTAGAGQSAVRSFRHKNFPWLGNTLGKTDVIYAPFEPIRIKD
                     NVAHVVLRDYRMNGFGLWNSVQSEGKEILAAPIALHVDTEKGEAGWRFGKGRWTSSGK
                     TLAVYQAGAESDAVKVKTTSSIEYDGCMKVEMELLPGKTPETIQHLWLEIPIRDEVAS
                     LFHYTAFESMRRNYAGKTPRGGKIVWMNQPYDQDPPKWPRGALPPIWKAEPGSDDGIL
                     WTCRDIRPWKHVLSTDFVPYIWLGGGERGLAFFGANDKGYLVDPNGTMQTIIRKGNTL
                     YLRVDLVNIPSVITKSRHITFGLQASPTRPMPENWRVKQKITPAMPGPVLAWGSYICA
                     DKYPESYHWQVVDEMIKIRETGIIDSTVFVKLDQERGEPWKKPWGGTGKAGFDKWFAL
                     DMWFFINQAKSLHDRNDWKKIYATGLYSEEDAKILANPTWMAYTEEHASDITEPEWEI
                     FQDEWRAEWPWKNPRSEITGQQQPDNNFKYGNQAFPESYLDFCLYYHNLWFTRGIGIY
                     FDNTMPYTMYNPLLSDAYYDETGKLQPASSIWQQRTYYKRVWQLMNEVQQKGVPYPIA
                     FAQHITNTRLLPLNSWNTTSLDIEWDWYRDTTQAAAPPSATNNGNAQTRRRTRLRLPV
                     PADLLLTETAGRQTGSMGDSHFGILGNNLGPGVPKTYSQSEWGMRVVHELKLHGAPVL
                     EAALWKFGYGTDKARVINYWSDNPPVRVNDPENNKWILLERPSDKSLFLVLQTWKKAD
                     EEVNVNIDFSKIGFTPAAKARDVATGGELPFTGPDLKLALPGPYGTRVIMIGSAAGN"
BASE COUNT         1167 a         1109 c         1211 g          966 t
ORIGIN      
        1 cggtctatca ggctggccgg acactggtag tatggcctta cagtgacaat gtcccctcga
       61 tcatgctgat gcatagggcg cggtggggag aatggataga gcaacgcatg cccgccgccg
      121 accgcatcaa gccgggagag gagatagaaa cggctaccca gtttttccgc ctcaacgagg
      181 gcccgatcat gccgtcaatg aatcattttc aaaaattctg gaaggacgtt aacgtccgcc
      241 tgcaggatag cactccggat tggggcaaag atgcgcggat ctatgaagtg gaggtgggac
      301 agaaaagaaa tcccgaaagc tcacgcactc cctatacatc catggcagaa ctggccagtg
      361 acctgcccag gattcagaaa atgggattta atattattga actcatgccg cacatgcctt
      421 tccccagcta ttcggtgcat gattatttta atatcgaagt gcagtacgga ccggaaaagg
      481 aattgcgggc catgatcgat caggcgcacc aactgggcgt aaaagttttc ctggatattg
      541 ttctgcacgg ggtcaccgac aaaaccctta accccctggc catttttgat aaaaacccat
      601 tggtcaaaga gcatccggac tggttcagtc acaatgaata tggggaaatc gcgaggacct
      661 atacctgggc attcgaccac cgcaacccgg aagttcagca atacatggtg aaggttttta
      721 atttttacgt ggataaactg aacccggacg gattccgcgt agatgccgtc acctggaact
      781 gctttcccaa ctggtctgaa aatttgaacc ggccggctta ttcaagtttc tacggcaccg
      841 cttcgatgat ggacctggtg agaaaagaaa cccataagat caaaccggaa cttgtctttt
      901 ataatgaagc ggcgggacct ctgtatttcc gcgcttttga tctctcttac aattatgacg
      961 agcaatggct gtatgcggcc atactatccg gtcgccggac ggggcaaggg cctggtcaga
     1021 gccgtcgcgg cgccggctgg actgaacccg gaatgagcgc ccaggacctg ggagaatggc
     1081 tcgaaatgcg ccgccgggct atgcctaaag gattgatcag ggtccatcac gtggattcgc
     1141 acgatgccta cgatgggagc ggctcccgtt ttcgccggga gatcctgggt ataccggcca
     1201 cgaggatgtt gtttgccatg acctctttta tcgacggagg agtaatgact tttaaaggcg
     1261 ccgagctcgg ttcggaggac tactattcca aagtcctttc cataagggaa tctattcccg
     1321 cgctcaaatc gggcacctgc gaatacctga ccatcacctc ggaaaacgag cgcgtgttca
     1381 cgccgcttcg ccggctcggg aatgaatggg cattgccggt gatatcgttt tcgcgtgaac
     1441 cgatcgatac ctggatatcc ctggctccgt taaaacttaa ccgggatagt gtctatacgc
     1501 tccgggaagc tttttccgga atggaaaaaa caggtacagg ccgtgacctg gcaagcctga
     1561 atgtaaaact ggattcgctc ggcgttcagg tctggacatt gaaaaactga tctgaaaaaa
     1621 acaattcatg cggtggataa atggcagtct ttcatttaaa cgtaaataaa ttaaatcaac
     1681 gtggggataa aaaaaataat atcattcatt taccggcagg agataagaat gatgatatgc
     1741 ctggccgcct gtttgataac agcgagcccg atcctgaatg cccagtccgg ttctgctagt
     1801 gcggcattgg attcagcgac ccgttttgcc tactatcccg gcctgcataa gctggaggtc
     1861 cggatcagtg atctgcctgc tgaagtattg gccggtcacc aggaagcgac tgcagtgatc
     1921 agtggaccgg gcgggaaaaa aacaatagcc cgccaggtgt tttccataac cggtaagggc
     1981 ggttcctgca tgatcagtct gcctccgttg gcggacggtg agtatgaact gatggtaaca
     2041 gccggagccg gtcagagcgc cgtgagatca ttcaggcata aaaattttcc ctggctcggg
     2101 aataccctgg gcaagaccga tgtcatttat gctccctttg aacccatccg cataaaagac
     2161 aacgtggctc acgtggtgtt gcgtgactac cgtatgaatg gttttggttt atggaacagc
     2221 gtacaaagcg aaggaaagga aatccttgct gcacccattg cgctgcatgt ggacactgaa
     2281 aaaggagaag caggctggcg ctttggcaaa ggcagatgga cctcatcggg caagactctt
     2341 gcggtgtatc aggccggggc cgaatcggat gcggtgaaag tgaagaccac ttcttcgatc
     2401 gaatacgacg gatgcatgaa ggtggagatg gaattgttgc cgggtaaaac acccgagacc
     2461 attcagcacc tctggctgga gattcccatc agggatgaag tagcctccct gtttcattat
     2521 acggcatttg aaagtatgcg ccgcaactat gccggaaaga cgccgcgcgg aggaaaaata
     2581 gtttggatga accagcccta tgatcaggat ccgccgaaat ggcccagagg cgcattgcct
     2641 cccatatgga aagcggaacc ggggagtgat gacggaatcc tttggacctg ccgggatatc
     2701 cgtccatgga aacacgtgct ctccaccgat ttcgttccct atatatggtt gggcggcggg
     2761 gagcgcggtc tggccttttt cggagccaac gataagggat acctggttga tccaaacggt
     2821 acgatgcaaa cgatcatacg gaaaggtaat accctgtatt tgcgggtcga cctcgtcaat
     2881 ataccctccg ttattacaaa atccaggcat attaccttcg gtctgcaggc aagtcccacc
     2941 aggccgatgc cggaaaattg gcgcgtaaaa caaaagatca caccggccat gcccggtccg
     3001 gtactggcct ggggtagtta tatctgtgcc gacaaatacc ccgagagtta tcactggcag
     3061 gtggtggatg agatgatcaa aataagggag accggaatca ttgacagcac ggtattcgta
     3121 aagctggacc aggagcgcgg cgaaccctgg aagaaaccct ggggtggcac aggcaaggca
     3181 ggattcgaca aatggtttgc cctcgacatg tggtttttta tcaatcaggc aaaaagcctg
     3241 cacgaccgca atgactggaa aaagatctat gctaccggtc tgtattcgga agaagatgca
     3301 aaaatactgg ccaatcccac ctggatggct tacacggaag agcatgcttc ggacattaca
     3361 gaaccggaat gggaaatatt ccaggacgaa tggcgggcgg aatggccctg gaaaaatcca
     3421 cgcagcgaga ttacgggtca gcagcaaccg gacaacaatt tcaaatacgg aaatcaggca
     3481 ttccccgaga gctacctgga tttttgcctg tattaccata acctgtggtt tacgcgaggt
     3541 atcggaattt atttcgataa caccatgcct tataccatgt acaatcccct gctctccgat
     3601 gcctactatg acgagaccgg caagcttcag ccggcatcat ccatttggca gcagcgaacc
     3661 tattataaac gcgtctggca gttaatgaac gaggttcagc agaaaggcgt gccgtacccg
     3721 atcgcttttg cgcagcacat caccaatacc cggttgctgc ccctcaatag ctggaacact
     3781 acttctctcg acatcgaatg ggactggtac cgggatacca cgcaggctgc ggcgcccccg
     3841 tccgccacaa ataacgggaa cgcacaaacg agacggagaa cgcggctgag gttgccggtg
     3901 ccggcggatc tgttattgac tgaaacggcc ggccgccaga ccggcagcat gggagattct
     3961 catttcggca tcctgggaaa taacctggga cctggtgttc cgaaaaccta ttcacagagc
     4021 gaatggggaa tgcgcgtcgt gcatgaatta aaactccacg gtgctcctgt cctggaagcc
     4081 gcattatgga aattcggata tggtaccgac aaggccaggg tgatcaacta ctggtcggat
     4141 aatccgccgg tacgtgtcaa tgacccggag aataataaat ggatcctgct ggaaaggcct
     4201 tccgacaaaa gcctctttct cgttctgcag acctggaaaa aagcggatga agaagtaaac
     4261 gtcaatattg atttttcaaa aatagggttc accccggcag cgaaggcccg cgatgttgcc
     4321 accggcgggg agctgccgtt caccggcccg gatcttaagc tggcattgcc ggggccctat
     4381 ggcacccgcg taataatgat cggatcggca gccggaaatt gagccggatg attaaaacga
     4441 ttaaaacgga taa
//
LOCUS       DASYCU010000180         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2360403, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000180 DASYCU010000000
VERSION     DASYCU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360403"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VGM24_03650"
     CDS             complement(<1..947)
                     /locus_tag="VGM24_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154487.1"
                     /translation="MIPELRKSYNQSFSEAKYQAFLKDLKSKHPGSIDFRIAETPVFV
                     PAGFQEKMISACESIVDLIAAPEFKDQSRNAIPAADFVPGNESPCSFIAFDFGVCLNE
                     EQELEPQLIEMQGFPTLYAFQVYYPEILRNHFALPENYTPYLGGYTRESYLEELKKLI
                     LGNAAPEQVVLLEIKPHEQKTRIDFYLTQDYLGIQPVCLTELIGEGNRLYYVHEQTKK
                     KTRIERIYNRIIFDDLQAQQDKLGPHVSIQKEWDVHWIPHPNWFYRISKHTLPFIRHP
                     QVPKTFFLNEVKQIPDDLENYVLKPLFSFAGQGVLIDVR"
     gene            complement(966..>1531)
                     /locus_tag="VGM24_03655"
     CDS             complement(966..>1531)
                     /locus_tag="VGM24_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HEY4154488.1"
                     /translation="HGMVKHNIVAADSDKKFLLFICHSFQLACRFFQTGKVTRRRSTA
                     FGVFPVHMKADGRKEPVFNGLKDPFYAVDSRDFQVIEEDRFNIKKKGAGILAIEKERP
                     HVPLQRAIMAIRFNEYMLGTQFHPEADPLGMSMYLQRADKKKTVIEGYGEEKWKSMIQ
                     HLNDPDKIRNTYSRVIPNFLNQARGRS"
BASE COUNT          355 a          365 c          374 g          437 t
ORIGIN      
        1 tttcttacat caatgagcac tccctgaccc gcgaaagaaa aaagcggttt gagtacgtag
       61 ttttccaggt catccggaat ctgtttgacc tcgttcagaa aaaaggtttt tggtacctgg
      121 ggatgccgga tgaacggaag cgtatgtttg gaaatgcggt agaaccagtt gggatgcgga
      181 atccaatgta catcccattc cttttgtatg gaaacatggg gtcctagttt atcctgctgc
      241 gcctgcaaat cgtcaaaaat gatccggttg taaatgcgtt cgatgcgggt tttctttttc
      301 gtttgttcat gcacgtaata caaccggttt ccttctccaa tcagttcagt caggcagacc
      361 ggttgtattc ccagatagtc ctgcgtgagg taaaagtcga tccgtgtttt ctgctcgtgg
      421 ggttttatct ccagcaaaac aacctgttcc ggtgcggcgt tgcccaggat cagctttttc
      481 agttcttcca ggtaggattc ccgggtatat ccgccgaggt aaggcgtata attttcgggg
      541 agggcaaaat gattgcgcag gatctccggg taatagacct gaaaggcgta cagggtggga
      601 aatccctgca tttcgatcag ctgcggctcc agttcctgtt cttcatttaa acaaaccccg
      661 aaatcaaacg caataaaaga acagggcgat tcatttcccg gtacgaaatc agcagccgga
      721 atggcattac ggctctggtc tttgaattcc ggggcagcga tcaggtctac aatggattcg
      781 caggcgctga tcattttttc ctggaaaccg gcgggaacga aaacgggcgt ttctgcaatc
      841 cggaaatcaa tgctcccggg atgttttgat ttcaggtctt tcagaaaggc ctggtatttc
      901 gcctcactga acgactggtt gtaagatttg cgcaattcgg gaatcatgat ttttgctttg
      961 ccggatcagc ttctcccgcg ggcctggttc aaaaaattcg ggatcacccg ggaataggta
     1021 ttccttattt tatccggatc gttcaggtgc tggatcatgc tcttccattt ttcttcccca
     1081 tatccttcaa tcaccgtttt ctttttatcc gcccgctgca ggtacatgct catgcccagc
     1141 ggatccgctt ccggatggaa ctgtgtgccc agcatatatt cattgaaacg gatcgccatg
     1201 atggcccgtt gaagcggtac atggggcctt tccttttcga tggccaggat accggcccct
     1261 ttctttttaa tgttaaaccg gtcctcttca attacctgga aatcacggct gtccacggca
     1321 tagaacggat cctttaatcc attgaataca ggttcttttc ttccatcggc tttcatgtga
     1381 acgggaaata cgccaaatgc ggtcgatcgc ctccgggtca ctttgccggt ctgaaaaaac
     1441 cggcacgcga gttggaagga atgacagata aacaacagga attttttatc ggaatcagca
     1501 gctacgatat tgtgttttac cattccgtgc a
//
LOCUS       DASYCU010000181         3442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1201032, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000181 DASYCU010000000
VERSION     DASYCU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201032"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..1982
                     /locus_tag="VGM24_03660"
     CDS             432..1982
                     /locus_tag="VGM24_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY4154489.1"
                     /translation="MKKFFVFLLSALFFQTLPAQKETALAEIEKEKPNAFIREGIDRY
                     IESGLKDWNLPGLAVVIVKDGKVVLMKGYGVKDIQSGAPVDTNTLFMIASNTKLFTAS
                     SLALLETRGSISLNDPVTKYFPHYRLYDSVSTKLVSLRDLLSHRIGTKTFQGDFTFWN
                     TSLSRTEIMKRMRLLKPSKPFRQDFGYCNSCFLVAGQVIPVVTGESWENFVQTKLLDP
                     LRMSHTLILSTGVAGQPDVATPYTSSYSRKLNRVPYDNWNNLAPAASIVSTVSDLSHW
                     LEMQLDSGRYEGRQVLPWAAVQKTRDINTIVSSRKSAVLPMHFTGYGLGLFAGDYAGR
                     QIYWHTGGAGGMVSNVCFVPEERLGIAILTNNDNQNFYEALRYQILDSYLGVPYVNRS
                     RQSLAGFEKTAGEQLSEIDHWKEDMKGNKPELPLASYTGVYSNPLYGTIKLKQEKNQL
                     IINFNTKPDLSATLDYLDQGDWLLTYNNIEYGIFKVRFALKNGKVRSLITKQNPFVEH
                     DPYVFTKE"
     gene            2036..2605
                     /locus_tag="VGM24_03665"
     CDS             2036..2605
                     /locus_tag="VGM24_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY4154490.1"
                     /translation="MEELFNTLSPLIRLSPESSSAFSAILVRMELKKGEILLKSERVC
                     NQMYYIEKGLARTFYLKDGKDVTDWISTEKNFSVSILSFITRKPDRRSIELLEDSVLW
                     SIHYDDLQKLYKEYHEIERLGRLLADEGLVQIQQRFDDLHFTSAQDRYKKLLLANPTI
                     IQRVPLGMVASYLGISQETLSRIRSGLSF"
     gene            2661..3029
                     /locus_tag="VGM24_03670"
     CDS             2661..3029
                     /locus_tag="VGM24_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4154491.1"
                     /translation="MNTLLWVFQVFLAVTFVYSGIMKSTSNREKLVRIGQTGIANFSY
                     PAIRSIGILEILGAFGILLPWRLQILPVLTPISSVCFAAIMIPAAGIHMRRKEFKSLS
                     FNILLFGISVFVAFKRFPVH"
     regulatory      3068..3139
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT          911 a          831 c          778 g          922 t
ORIGIN      
        1 cccgcaaggg aagcctgttt tacaaatgct cttcttgaat ttttcatatc gttgatttag
       61 taaataatgt aataatcgcc ccaaattatc ccataaactt tttaattcac cggaagcgga
      121 ataaatatcc gcaattctga agacagaccc ggaaatcagg agaacgccct cccgctctat
      181 ccaattgatt aataaatgta tcgaaaatca ggcgttctca aaaacggggc tggtacgggg
      241 attgaatgag tgaattggtt tttcatagga tattggatta acccgggccg gatttctatc
      301 cggcccttct cttttttgcg accggaggct ttaatgtagc gcaggataaa taaatttgca
      361 ggtcttgttt tcttaaaccg gctcccacaa aataaccttt gcccgggcag ccatttattt
      421 aattttattt gatgaaaaaa ttcttcgtct tcctcctgtc cgcccttttt tttcaaaccc
      481 tccccgcgca aaaagagacc gctttagcgg aaatagaaaa ggaaaagccg aatgctttta
      541 tccgggaagg tatcgaccgg tatatagaaa gcggactaaa ggactggaac ctgccgggcc
      601 tcgccgtggt tattgtaaag gacggaaaag tggtgctgat gaaaggttat ggggtaaagg
      661 acattcaatc cggagcgccg gtggatacca ataccctgtt catgatcgcc agcaatacca
      721 agctgttcac cgccagttcg cttgccttac tggaaacacg gggcagcatt tcgctgaatg
      781 atccggtcac taaatatttc ccgcactatc gtttgtacga ttccgtcagt acaaaactgg
      841 tcagcctccg cgacctgctc agtcaccgca tcggcaccaa aaccttccag ggcgacttta
      901 ccttctggaa cacttccctt agccgcacag aaattatgaa aaggatgcgg ttgctgaaac
      961 cgtcaaagcc tttccggcag gatttcggct actgcaacag ctgcttcctg gtagccggac
     1021 aggtcattcc ggtggttacc ggcgaatcct gggaaaattt tgtgcaaacg aaattgctgg
     1081 atccgctccg gatgagccat accctgatcc tgtccactgg tgtcgccggg cagccggatg
     1141 tggcgactcc ctataccagt tcctattccc ggaaattaaa cagggttcca tacgataact
     1201 ggaacaatct ggcgcccgcg gcgagtatcg tcagtaccgt aagcgatctc tcccactggc
     1261 tcgagatgca gctcgacagc ggccggtatg aaggccggca ggttctcccc tgggcggccg
     1321 ttcaaaaaac cagggatatc aatacgatcg tcagcagccg gaagtccgcc gttttaccga
     1381 tgcattttac cggctatggt ctcggtttgt ttgccggaga ctatgcgggc cgccagattt
     1441 actggcatac cggcggcgca ggtggcatgg tgagcaatgt ttgttttgtt ccggaggaaa
     1501 ggctcggcat tgccatcctg acaaacaatg acaatcaaaa tttttatgaa gccctgcggt
     1561 atcagattct ggattcttac ctgggcgttc cgtatgtaaa caggagccgg caatcactgg
     1621 cgggatttga aaagacggcc ggagaacagc tttccgaaat tgatcactgg aaggaagata
     1681 tgaagggaaa taaaccggaa cttccactgg catcctatac cggcgtttat agtaacccgt
     1741 tatacggaac gattaaacta aagcaggaaa aaaatcagct catcataaac ttcaacacca
     1801 aaccggatct ctctgcaaca ctggactacc tcgatcaggg cgactggctg ctaacctaca
     1861 ataatataga atacggcata tttaaagtcc gatttgccct taagaacgga aaggtccgtt
     1921 ccctaatcac aaagcagaac ccttttgttg agcatgatcc ctacgtcttt accaaagaat
     1981 aaatggcaga ggccatatga tatccggttt atcctaataa cctgatttga aacgaatgga
     2041 agaattattc aataccctca gccccttaat caggctttct cctgaaagca gctccgcatt
     2101 ttcggccatc ctggtcagaa tggagttgaa aaaaggggag atcctgttga aatccgaaag
     2161 ggtctgcaat cagatgtatt atatcgagaa ggggctcgcc cggacctttt atctgaaaga
     2221 cggcaaagac gtgacggact ggatcagcac ggaaaaaaac ttttctgttt ccattctgag
     2281 ctttattacc cgcaaaccgg accggcgatc catcgaattg ctcgaagact ccgtcctctg
     2341 gtccatccat tacgacgatc tgcaaaaatt gtataaggaa tatcatgaaa tcgaacggct
     2401 tggaagactc ctggcagacg agggcctggt gcagatccag caacgattcg acgatcttca
     2461 ttttacttcc gcacaggacc ggtacaaaaa attattactg gccaatccaa ccatcattca
     2521 gcgtgttccc ctgggaatgg tcgcgtccta tctcggcata tcccaggaaa cattaagccg
     2581 catccggtcc ggcttaagtt tttgacatta gtcaaaggaa acctgccgcc gtattcaaac
     2641 ctttgtggaa aatcattttt atgaatacgc ttctttgggt cttccaggtc tttctcgcgg
     2701 tcacttttgt ctattccgga atcatgaaat ccaccagtaa ccgggaaaag ctggtccgga
     2761 tcggccaaac gggcattgcg aatttttcct atccggccat ccggtctatt ggcatcctgg
     2821 aaatactggg agcttttggt atcctgttgc cctggcgcct tcaaatcctt cccgtactga
     2881 cgcccatttc ttcggtttgt tttgccgcca tcatgatacc ggcagccggc atccatatga
     2941 gaagaaaaga attcaaatcc ctttcgttca atatactgct gttcgggatc agtgtttttg
     3001 tggcttttaa acgattcccg gttcattaaa ggcagtcttt gaatttatta taaatccgta
     3061 ccttcggttt tgcaaaggaa atggtgtctt cctatctaac cgtttcagat tccctgaagc
     3121 taatggcgcc tacgaattat taacccaatg tttaataata atagtttgta ggaatgcata
     3181 agctggattt ttcacgcaaa cttcactttt ttttatctct tttgaagaaa gccctaccgg
     3241 gcatggcatt cgttcccttc tctttattca atggggcatg gaatgaaata atgtttaaac
     3301 gataaaaaat gaaactggta ttcatcgtcg ggttgggtgg cggaatcggc agcatgctaa
     3361 gatatctgat gcagattttt gtaagccgtc atatgcccat tgtttttccc tggggaacat
     3421 ttcttgtaaa ttgctccggc tg
//
LOCUS       DASYCU010000182         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1639820, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000182 DASYCU010000000
VERSION     DASYCU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639820"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGM24_03675"
     CDS             <1..195
                     /locus_tag="VGM24_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY4154492.1"
                     /translation="DLKNSYHELVIRNTGRLDERNREEGFGLMSTRNRLQLLFGDQAN
                     FDIREVNGNTVEAVLHIPLT"
     gene            207..947
                     /locus_tag="VGM24_03680"
     CDS             207..947
                     /locus_tag="VGM24_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4154493.1"
                     /translation="MIRAVIIDDERLARNELKKLLLEFPEIEVIAEAANANEGIEKID
                     SLNPDLIFLDIQMPGKTGFDMLAELEKAPDVIFTTAYDDYALKAFEVNALDYLLKPVE
                     PRRLADALQKLHLEETRDREGMNDEEISVNKTLLHETDQVFVKDGERCWFVKLADIRL
                     FESVGNYAKIYFGTNKPLILKSLNALEERLDEKVFFRANRKHIVNLRLIEKIEPYFNG
                     GLLLDLKGGEKIEVSRRQTVKFKEMMSL"
     gene            952..>1744
                     /locus_tag="VGM24_03685"
     CDS             952..>1744
                     /locus_tag="VGM24_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY4154494.1"
                     /translation="MQTPIRSLVFALLLFAGSPGLGQSPARLIRQDEVTRIEKILSAD
                     DMQGRQVFTPGIEKAAAFIIREFKSAGLKPLPGSKDFRQPFTLIKTKRIEIRANLDGR
                     EIDSNQLTVLSAAADLSISPADHYQKAFIKKGDDFTAAFFKFLETDQNFIILVDTVFS
                     KRFARLSAFQMPQLEGSGNRIFILTNRDPDQYSIQIKQEISRQQLSNIAGILPGRSLK
                     NEYVIFSAHYDHLGTGTADAAGDTVFNGANDDASGVAAVISLANYF"
BASE COUNT          489 a          407 c          451 g          397 t
ORIGIN      
        1 gatctgaaga acagttatca cgagctggtg atccgcaaca cggggcgcct ggatgagcgc
       61 aaccgggaag agggatttgg attgatgagc acccgtaacc ggctccagtt gctttttgga
      121 gatcaggcaa attttgatat ccgtgaagtg aacggaaata cagtggaagc ggttttacac
      181 attcccctga cttaaaacaa aaaaatatga tcagagcagt tatcattgac gacgaaaggc
      241 ttgcccggaa tgaattaaaa aaactcctgc tggaattccc ggaaatagaa gtgatcgcgg
      301 aagccgccaa tgccaatgag ggaattgaaa agattgacag cctgaatccg gatctcattt
      361 ttctggacat ccagatgccg gggaagaccg gctttgatat gctggctgaa ctggaaaaag
      421 cacccgatgt gatttttacg acagcttacg atgactatgc cctgaaggcg tttgaagtga
      481 atgctctgga ttatctgctg aaaccggtgg aacccaggcg gcttgccgat gccctgcaga
      541 aactgcacct ggaagaaacc cgcgaccggg aaggaatgaa cgacgaagaa atttccgtaa
      601 ataagaccct gctgcatgaa accgatcagg tattcgtgaa ggacggggag cgctgctggt
      661 ttgtaaaact ggcggatatc cggttgtttg agagcgtggg aaactatgcc aagatttact
      721 ttggcaccaa taaacccctc atcctgaaat ctctcaacgc cctggaagaa agactggatg
      781 aaaaagtttt tttccgggct aaccggaaac acatcgtgaa tctccggctg attgagaaaa
      841 tagaacctta ttttaacgga ggcctgttac tggacctgaa gggaggggaa aaaattgaag
      901 tcagccgaag acaaacggta aagtttaaag aaatgatgag tttataaccc gatgcagact
      961 cccataagat cgctggtttt cgccctgctg ctgttcgccg gcagcccggg tttgggacaa
     1021 tcgcccgccc gcctgatccg gcaggatgaa gtcacccgca tcgaaaaaat cctgtctgcc
     1081 gacgacatgc agggcaggca ggtatttacc cccggtattg aaaaggccgc cgcgttcatt
     1141 atccgggaat ttaaatccgc cggattaaaa ccgctgcccg gaagcaaaga tttcagacag
     1201 ccgtttaccc tgatcaaaac aaaacggatt gaaatcaggg caaacctgga cggccgggag
     1261 atcgattcca atcagcttac ggttctttct gcagccgcgg atctgagcat tagccccgcc
     1321 gatcattatc aaaaagcatt cataaaaaag ggcgatgatt ttacggcagc cttttttaaa
     1381 ttcctggaaa cggatcagaa ttttattatc ctggtggata ccgttttttc aaaacggttt
     1441 gcccggctct ccgcatttca gatgccgcag cttgaaggtt cggggaaccg gatctttatt
     1501 ttaaccaacc gggatccgga tcagtactcg attcagatca aacaggaaat atccaggcag
     1561 caactgtcca atattgccgg tatcctgccc ggccggtcct taaaaaatga atatgtaatt
     1621 ttttccgctc attacgatca cctgggaacc ggaacggcgg atgccgcggg ggatacggtg
     1681 ttcaacggag ctaacgacga tgcttccggc gtggcggcgg tgatcagcct ggccaactat
     1741 ttcg
//
LOCUS       DASYCU010000183         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1201259, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000183 DASYCU010000000
VERSION     DASYCU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201259"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..991
                     /locus_tag="VGM24_03690"
     CDS             119..991
                     /locus_tag="VGM24_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4154495.1"
                     /translation="MNIPQTITLAGNSSHPLVVNRFGYGTMRLTGEGIYGEPKGRPEA
                     LKILKRAVESQLRFIDTADYYGEDVTNRLIAEALYPYPSDLVICTKVGATRKPDKSWI
                     PFNRPENLRSSIENNLRTLKLEKITLVHFRVMPGHTQFTESMEAMFRLQQEGKILHIG
                     VSNVTRAELQTAMKMGAIATVENMYGHDQRTTLKMAYGENRGGEEILDLCEQNEMPMV
                     PYWSIFNSRENNRIAAISKKYNVTEAQINIAWTLHRSPWILPIPGTSSLLHFEENLKA
                     REIKLTAEDMDYLE"
     gene            1069..1824
                     /locus_tag="VGM24_03695"
     CDS             1069..1824
                     /locus_tag="VGM24_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4154496.1"
                     /translation="MKSLLFTLLLTPVLYSGYAQRSAPDMLQTKMGPLSIQPIEHASL
                     ILTVHGLTIYADPVKPENYKGLKPPDIILITDIHGDHFDVKTIDAVKKNNTLLVVPQV
                     VADKLPAADKNHLVILHNGDQTTQSGISIRAIPMYNLPETPTAMHTKGRGNGYVLGIG
                     GRLIYLSGDTQGIPEMRALKNIDVAFVCMNLPYTMDIREAADAVLAFKPRIVYPYHYR
                     GNNGLSDVNAFKSLVEAGNKNIEVRLRNWYPGK"
     gene            complement(1840..3108)
                     /locus_tag="VGM24_03700"
     CDS             complement(1840..3108)
                     /locus_tag="VGM24_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4154497.1"
                     /translation="MNKKIIFTACLFVFLLIGLLSFRIIRTGHAGRYENEKINADLTL
                     PAGFQAVAIAENLGNARHMAVTPKGDIYVHLGGLKNGKGIVVLHDNGDKADIKSGFGS
                     FAGTGIRIKNGYLYASSDRDVYRFKLNKDEEVIDPGHPEKIVSNLVSKRMHQPKAIAL
                     DNNGYIYVNIGAYSNICSDFDPQKKGCPVLDSAAGIWRFKADRLNQKQSDGIRYATGL
                     RNVVGMDWNQQDNQLFVMQHGRDALHNLFPKLYTDQQSALLPAECMYALKEGDDAGWP
                     YMYYDDVKHKKILGPEYGGDGIKEANSKYLNPVAAYPGHLAPDGLLFYTGNQFPAKYK
                     NGAFIAFHGSWNRAPEPQAGYFVVFQPFKNGRPSGKWEVFADGFAGSPEQKASGRAEH
                     RPCGLAQAPDGSIYVSDDNKGTIYKISYKK"
     gene            complement(3132..>3834)
                     /locus_tag="VGM24_03705"
     CDS             complement(3132..>3834)
                     /locus_tag="VGM24_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HEY4154498.1"
                     /translation="TPVPPIDSVTVVYDSSLITDIRPTQLIVSVNTHDPPGVRNYYRW
                     TASGYVPRKSWGDTCNIFVIIPCRDPFDCSCGALCEQFLKYNPVNVFSDQFIDGNSIV
                     LPVFYSPVYWFGKHFIEIKQYSLSREADEFWQQYLDQTNRTGSILDPLPASLAGNLHN
                     QSDARDIALGFFSASDVFTKKLVIVPFFLQQYWLVSTAGAYVKEGQCELVYPNTLPNN
                     ADAPGWEHAQTIEMH"
BASE COUNT          998 a          962 c          921 g          953 t
ORIGIN      
        1 atatccgttt ccatgaaatg gcctgtccgg ttcctcttta ttatatttgt ggccggtttt
       61 acccttgcct ggtaattgta tccctgatct ttttgtccgg cccacctaaa taattctcat
      121 gaacattcca cagacaatca ctcttgcagg aaacagcagc catccgctgg tcgttaaccg
      181 ttttggttat ggaacgatgc gccttaccgg ggaaggaata tatggcgaac cgaagggcag
      241 gccggaggcg ttgaaaatat taaaacgggc ggttgaaagc caattgcggt ttatcgatac
      301 ggcggattac tatggggaag acgtcaccaa ccgcctcatt gccgaagcac tataccccta
      361 cccttccgac ctggtcatct gcacaaaggt cggagcgaca agaaaaccgg ataaaagctg
      421 gatacccttt aatcgtccag aaaacctgcg cagcagtatt gaaaacaatt tgcggacctt
      481 aaagcttgaa aaaataaccc tggttcattt ccgggtaatg ccgggccata ctcaatttac
      541 agaatccatg gaagcgatgt tcaggctgca gcaggaggga aaaattctgc atatcggggt
      601 cagcaatgtt acgcgagccg aacttcaaac ggcaatgaaa atgggcgcca ttgccaccgt
      661 ggaaaatatg tacggtcacg accagcgcac gaccctgaaa atggcttacg gagagaaccg
      721 gggaggagaa gaaatactcg acctctgtga acaaaacgaa atgcccatgg tcccctattg
      781 gtccatattc aactccaggg aaaataaccg gatagcggcc atcagtaaaa agtataatgt
      841 aacggaggcc cagattaata ttgcctggac acttcaccgt tccccctgga tattacccat
      901 acccggcacc tcttccttat tgcactttga ggagaatctg aaagcaaggg aaataaaact
      961 gacggcggaa gatatggact acctggaata agatgctccg ttcccggatc ctgactttat
     1021 ttgccttttt acttttcagt atatttaccg gtacctaatt catcaattat gaaatccctg
     1081 ctttttaccc ttttgcttac gccggtatta tattccggat acgcgcaacg gagcgctccc
     1141 gatatgcttc aaacgaagat gggtcctctc agcatacaac ccattgaaca tgccagcctg
     1201 atcctcaccg tgcatggctt aacgatctat gctgatccgg taaagccgga aaattataaa
     1261 ggactgaagc ctcccgatat tatcctgatt acagacatac atggcgatca ctttgatgtg
     1321 aagaccattg atgcggtcaa aaagaacaat accctgctgg tagtgcccca ggttgtggcg
     1381 gataaactgc ctgccgccga taaaaatcac ctggtaatac tccacaacgg agatcaaacc
     1441 acgcaatccg gtatttccat ccgggccatc ccgatgtata atttgccgga gactccgacg
     1501 gccatgcata ccaaaggccg cggcaatggt tatgtgctcg ggataggcgg ccggctcatt
     1561 tatctatctg gtgatacgca gggaattcct gaaatgaggg ctctgaaaaa tattgatgta
     1621 gccttcgtat gcatgaacct tccctatacc atggacatca gggaagcggc cgatgcggtg
     1681 ctggccttta aaccgcgcat cgtttatccc tatcactacc ggggcaataa cgggttgagc
     1741 gatgtcaacg cctttaaaag cctggtggag gccggaaata agaatataga agtgcggctg
     1801 cggaactggt atcccggtaa ataacaggcc gccagattat tatttcttat agctgatttt
     1861 ataaatggtt cctttattgt catcgcttac atagattgat ccgtccggcg cctgggcaag
     1921 accgcagggg cggtgttctg cccggcccga agctttctgt tccgggctgc cggcaaaacc
     1981 gtcggcaaag acttcccact taccggaggg tcttccgttt ttgaaaggct ggaagaccac
     2041 aaaatatccc gcctgcggtt ccggcgcccg gttccaggac ccatgaaatg cgatgaaggc
     2101 tccgttctta tattttgcgg ggaactgatt gcccgtatag aataacaaac cgtccggggc
     2161 caggtgaccg ggataagcgg caaccggatt cagatatttg gaatttgctt ctttgatgcc
     2221 gtcccctcca tattccgggc ctaatatttt tttatgtttt acgtcatcat aatacatgta
     2281 cggccatccg gcatcgtctc cttctttaag cgcatacatg cattcggcag gcaaaagagc
     2341 cgattgctga tccgtatata gtttaggaaa aagattgtgc agggcatccc ggccatgctg
     2401 catcacaaaa agctggttat cctgctggtt ccaatccatt cccaccacat tgcgcaggcc
     2461 ggtcgcatag cggatgccgt cgctttgttt ctggtttaag cggtctgcct tgaaccgcca
     2521 tattccggcg gcagaatcca aaaccgggca tccttttttc tgcgggtcaa agtcactgca
     2581 aatattcgaa taggccccaa tgttcacata tatataaccg ttattatcca gggcgatcgc
     2641 cttcggctgg tgcattcttt tactgaccag gttactgact atcttttcgg gatggccggg
     2701 atcaatcact tcttcgtcct tgttcaattt aaaccggtat acatcgcgat ccgacgacgc
     2761 atacaggtat ccgttcttaa tgcgtatccc cgttccggca aatgaaccaa aaccggattt
     2821 tatgtcggcc ttatctccat tgtcgtgtaa aacgacaatt cctttcccgt ttttaagtcc
     2881 cccgagatgc acataaatat cacccttagg cgtaaccgcc atatgccggg cattaccgag
     2941 attctcggcg atggcaacgg cctgaaatcc cgccggaagg gttaggtctg catttatttt
     3001 ttcattttca taccggccgg catgcccggt ccggatgatc cggaaagaga gcagtccgat
     3061 caaaaggaaa acgaaaaggc aggcggtaaa aattatcttc ttattcataa cgcgtgattt
     3121 gatactgcaa tttagtgcat ctcgatggtt tgcgcatgct cccagccggg agcatcggca
     3181 ttgttgggaa gggtattggg atagacgagt tcacactggc cttcctttac ataagcaccc
     3241 gcggtgctga ccagccagta ttgctgaaga aaaaacggga cgatcaccag tttctttgta
     3301 aagacgtcgg aggccgaaaa aaaacccagt gcaatgtccc gggcatccga ctggttgtgc
     3361 aaattgcctg ccagcgaagc gggcaacgga tccaggatac ttccggtcct gttggtctgg
     3421 tccaggtatt gctgccagaa ttcgtcggcc tcccggctca gcgagtattg ttttatttct
     3481 ataaaatgtt tgccaaacca atagaccggg gaataaaaaa cgggcagtac aatcgaattg
     3541 ccgtcaatga actggtcaga aaatacattc accgggttat actttagaaa ttgttcgcat
     3601 aaggccccgc aggaacaatc aaacggatcc cggcagggaa ttataacgaa tatattacag
     3661 gtatctcccc aggatttgcg gggaacatag ccggaagccg tccaccggta ataattccgg
     3721 acaccgggcg gatcatgggt gttgaccgac acgatcagct gggtggggcg tatatcggta
     3781 attaaggaac tgtcgtacac caccgtcacg ctgtcgatcg gcggaacggg cgtt
//
LOCUS       DASYCU010000184         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1928585, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000184 DASYCU010000000
VERSION     DASYCU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928585"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..327
                     /locus_tag="VGM24_03710"
     CDS             70..327
                     /locus_tag="VGM24_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154499.1"
                     /translation="MMIRNKDELKEAIRELEHQNYVNEQQLRRRVSGIRDKLKPMNIV
                     KNLVSQAIGTTQVKTNIFRMVAGIATSFLVKKFFKKGIPAK"
     gene            424..>1939
                     /locus_tag="VGM24_03715"
     CDS             424..>1939
                     /locus_tag="VGM24_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HEY4154500.1"
                     /translation="MKKNKLLCCTLLSSFFFGVAVAQPFVRQSSGQVITVRADHPTAE
                     IQPTMWGVFFEDINMGADGGIYAELVKNRSFEFSKPMMGWTVLGKPATEGDFLVLNRR
                     QENTANPRYLRVTLHKNTKGSIGLSNEGFRGMGIKEGVRYDFSVQVRTSSGNNTLHVE
                     LVNSQGEIIGKASMPVAATGDQWKKQTLSFTAAATELRARMNIWFEGDGVMDLDMVSL
                     FPGDTWKKRPGGMRADMVQLLADMKPGFIRFPGGCIVEGPDLSQRYQWKKTIGPIEER
                     QLIVNRWNFEFAHRPAPDYFQTFGLGFFEYFQMAEDIGAEPLPILNCGMACQFNTAEA
                     VPLEDLDPYVQDALDLIGFANGDVNTKWGKVRAGMGHPDPFHLKMMGVGNENWGPQYI
                     ARLKVFTKAIREKYPDIKLVNSSGTDPNGDRFDYLNGELRQLHADIIDEHYYRRPEWF
                     FGNARRYDNYPRNGPKIFGGEYAAQSDHTVSVDNKNCWIAALSEAAFMTGLERNA"
BASE COUNT          516 a          439 c          524 g          460 t
ORIGIN      
        1 ccgcaaccgc tggctgaaag agcctttcag taatatgctg atccggaaaa tctttaataa
       61 gcagaactca tgatgatcag gaataaagat gaattgaagg aagcgatccg ggaactggaa
      121 caccagaatt atgtcaatga gcagcagctg cgcagaaggg tatccggcat ccgggataag
      181 cttaagccga tgaatattgt caagaacctg gtctctcagg caatagggac cactcaggtg
      241 aagacaaata tattccgcat ggtcgccggc attgccacca gcttcctggt aaagaaattt
      301 tttaaaaaag gcatacctgc caaataagaa gtagtcttat ctatatagta ccaaccaccc
      361 ctgtcaagca attgataggg gttttcgttt atctttacgg agcttaaaga ccaaacgaac
      421 tatatgaaga agaacaaact actctgctgt actctccttt catctttctt ttttggtgtt
      481 gccgtcgccc agccgtttgt acggcagtct tcggggcagg tgatcactgt gagggccgat
      541 catcctaccg ccgaaattca gccgacgatg tggggggtat tttttgagga catcaatatg
      601 ggcgccgatg gaggtattta tgcagagctg gtgaagaatc gttcattcga gttctccaaa
      661 cctatgatgg gctggacggt cctgggtaaa cctgccacgg aaggagattt cctggtactc
      721 aaccgccggc aggaaaatac ggccaatccc cgatatctga gggtcacctt acataaaaat
      781 acaaagggca gcatcgggct tagcaacgaa gggttccggg gcatgggcat aaaagaggga
      841 gtccgctatg atttctccgt ccaggtacgg acatcttccg ggaataatac gctgcacgtc
      901 gaattggtca attcccaggg agaaattata ggaaaagctt ccatgccggt tgccgccacc
      961 ggtgaccaat ggaagaagca gaccctatcc tttacagccg ccgcgacaga attacgtgcc
     1021 agaatgaata tctggtttga aggagatggt gtgatggatc tggatatggt ctctttattt
     1081 ccgggtgaca cctggaagaa aaggcccggc ggcatgcggg cggatatggt acagctcctg
     1141 gcagatatga aacccggctt tatccgtttt ccgggcggtt gtattgtgga aggtcccgat
     1201 ctttcgcagc gttatcaatg gaaaaagacc atcggcccga tagaagaaag acagttgatc
     1261 gtcaaccgct ggaattttga atttgcgcat cgtcctgcgc cggattattt tcagactttt
     1321 ggcctgggct ttttcgaata ttttcagatg gcggaagata tcggggcgga gccgctgccg
     1381 atcctcaact gcgggatggc ctgccagttc aataccgccg aagcggtacc tctggaggat
     1441 ctcgaccctt atgtacagga tgctcttgac ctgatcgggt ttgccaacgg ggatgtaaat
     1501 acgaaatggg gaaaggtccg tgccggcatg ggacatcccg acccctttca tttaaaaatg
     1561 atgggtgtgg gaaatgagaa ctggggacct caatacatag cccggttaaa agtgtttacg
     1621 aaggcgatca gggaaaaata cccggacatt aaactggtga acagctccgg caccgatccg
     1681 aacggagacc ggtttgatta cctcaacggg gaattgcgtc agttgcatgc ggatatcatc
     1741 gacgaacatt attaccgcag gcctgaatgg tttttcggga atgcccggcg gtatgataat
     1801 tatccgcgga atggtccgaa gatcttcggt ggggaatatg ccgcacagag tgaccacacc
     1861 gtgagcgtgg ataataagaa ctgctggatc gctgccttat cggaagcagc ttttatgacc
     1921 ggacttgaac gcaatgcag
//
LOCUS       DASYCU010000185         4794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_767956, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000185 DASYCU010000000
VERSION     DASYCU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767956"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VGM24_03720"
     CDS             complement(<1..1371)
                     /locus_tag="VGM24_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEY4154501.1"
                     /translation="MKLQTAYLLASAVMLGVFASCKKADLLKTNTDPNSVSVDVFDPN
                     NLLTATQLYYTGSTDNAIEVEETEIQGAGCFIQHWASTSGYFFGDKYLFSPGGWSSFF
                     DHAYTGAVKNVVDLYTTTIGKPQYANLHQISRIMKALVFERITDVYGDVPYFQAGLAY
                     HGSVYFPPYDKQQDIYTDLLKEVSEAVDSLDENADQPPGDMFYSQSGDQIDEWKKFGN
                     TLLLRMAMRLTKVDPATAQSFVTKVQGLTMTSNDDNAIVPHGTNDPLTINRIYRGIGE
                     DGSIQLSGQISKTFIDFLKSNDDPRLPVYSYVYPPDFQPGDPPNGGSDDPADQIGLPN
                     GYDEGNTAHGIINYPGYLGNTALYSRPGPIIFNPTAPTLILTYAESELLLADAAKRWG
                     IGDAEEHYNNGVLAAITELSAYGDAGVISDDDAQAYLDAHPYDDANGLQMINTQFWAA
                     TFFNEYE"
     gene            complement(1405..4428)
                     /locus_tag="VGM24_03725"
     CDS             complement(1405..4428)
                     /locus_tag="VGM24_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154502.1"
                     /translation="MAVAQNPPAVQVIKGRVTDVKTNEALAGVSVGIKGTTTLVQTDK
                     DGNYSISAGSTATLTFSYVGRKYEEVPVGSQTVINMRLEESAAQLTDVVVTALGIKRE
                     KRSLGYSVTTVAGSTLTEAREVNFVNNLEGKVAGVNVSNVATGPGGSANVVIRGISDI
                     TGSNQPLYVIDGIPMQNSTYRQTDVGGGYGGADGGDGTININPDDIETITVLKGAAAS
                     ALYGYRGSKGVIVITTKSGKNAKGTGVELNSNYVIQNVIDNTDWQKTYGQGSDGNKTV
                     DGDDAFETGLSSWGAKLDGSQVYQFDGVQRPYSAVSGNIGRFYKDGDAATNTLSFSKS
                     LGTDGSIRFSASDLDNTSLIPNAGFKQQSFSLSVNYKLDKHLDLQLKGQYLNAFTHNR
                     PSVSDAPGSVNFATLFLPPNVNIKDLAPGYKEDGSENQFLDDAYTTNPYFAAYKFINN
                     TRRNRFIGSADLKYSFDNGLYLEFRAGEDYFADRNTNVTPNGTAYQLDGFMQEENIKS
                     TELNVDGIIGKEFKIAKDFKLSALVGVNSRKAVVDQIDAQGNTFQTPYLYTIGNLASP
                     TEYTSNPVIENKSIYGTLDFSFKNYLYLTATGRNDWYSTLAPGKTNYFYPSVSGAFVF
                     SELVHIPAMDMGKLRVGYANVGGEADAPYQTLLGYNNVGNLNGYPIGNISNGGTVPNS
                     TLEPSSAKEFEVGTEMTFFNNRIHVDAAYYDKKESREVIPAVTSGTSGYDAVLLNSGT
                     IENKGIELLLSGVLVKAKNFSWTETLNLTYNDNKVLSLSTGSTNYPLGYSRAGEDEGI
                     GVAYMSQALGKSAYQIFVADPERDDKGNIVIDPLTGAPAAGTNYVDVGSGIDPWSTGI
                     NSEFRYKHFVLSFLIDAKFGGKIFSGTNYYAYQFGLSKATLPGRDLRYGTNQIYPQDY
                     YNLVSNANPDIFVYDASFVKFRQIIFGYNFPATLFRDKIQGITLSFVARNVFTITKHT
                     PDIDPESNYSNGPQGIEQAGVPYTRTFGLNLNVKF"
BASE COUNT         1138 a         1185 c         1181 g         1290 t
ORIGIN      
        1 ctcgtactcg ttaaagaagg tagcggccca aaactgggtg ttgatcatct gcagcccgtt
       61 ggcatcatca taaggatgcg cgtccaggta tgcctgtgca tcgtcgtcac tgatcacgcc
      121 agcgtcgcca taggccgaga gttcagtgat ggcggccagc accccattat tataatgttc
      181 ttctgcatcc ccgatgcccc agcgcttggc cgcatccgcc agcagtaatt cagattcggc
      241 atacgttaag atcagcgtcg gagctgtggg attaaatatg atcggaccgg ggcgtgagta
      301 cagggcggtg tttcccaggt aaccgggata attaataatg ccatgggccg tgttgccttc
      361 atcgtaccca ttgggcaggc cgatctggtc tgccggatca tccgatcctc cattgggagg
      421 atccccgggt tgaaagtccg gcggataaac ataggagtat accggcagac ggggatcgtc
      481 gttggatttt agaaagtcaa tgaatgtttt gctgatctgt ccggataatt gaatgctgcc
      541 atcctcgcct attccccggt agatcctgtt tatggtgagc ggatcattgg tgccgtgagg
      601 aacaatcgca ttgtcatcgt tgctcgtcat ggtaaggccc tgcactttcg ttacaaagga
      661 ctgcgccgta gccgggtcaa ctttggtcag gcgcatggcc atgcgcagca gcagggtatt
      721 tccaaatttt ttccattcat ctatctggtc gcccgactgg gaataaaaca tatcccccgg
      781 cggttgatct gcattttcgt caaggctgtc aacggcttcg gatacttcct tcagcaaatc
      841 ggtataaata tcctgttgtt tatcataggg aggaaaataa acagatccgt gataagccag
      901 cccggcctgg aagtagggca catccccata gacatccgtg atccgttcaa ataccagggc
      961 cttcattatc cgggagatct ggtgcaggtt ggcgtattgc ggcttgccga tggtggtggt
     1021 gtaaagatcc actacatttt ttaccgcgcc ggtatacgcg tgatcaaaaa atgaactcca
     1081 gccgcccggg ctaaacaggt atttatctcc aaagaaataa ccggaggtgg aagcccagtg
     1141 ctggataaag cagcccgcgc cctgtatttc ggtttcttcc acctctatgg cgttatcggt
     1201 actgcccgta taataaagct gcgttgccgt taataaattg ttggggtcaa aaacgtcgac
     1261 cgatactgag ttgggatcgg tatttgtttt cagcaggtct gcttttttgc acgaagcaaa
     1321 gacccccagc ataacggcgc ttgccagtaa gtatgcggtt tgtaatttca tattgagtag
     1381 cattatatct tttgaataat catcttaaaa tttcacgttc aggttcagcc cgaatgtccg
     1441 ggtatagggc actccggcct gttcgatgcc ctgcgggccg ttcgaatagt tggactcggg
     1501 atcgatatcg ggtgtgtgtt ttgtaatggt gaatacattc cgggcgacaa aacttaaggt
     1561 tatgccctgt atcttatccc ggaacagcgt ggccgggaaa ttatacccga atatgatctg
     1621 tctgaatttg acaaaactgg cgtcataaac aaatatatcc gggttggcat tactaacgag
     1681 gttgtaataa tcctgcggat agatctggtt ggtaccgtat ctcagatctc tgcccggcag
     1741 cgttgccttt gaaaggccga actggtaggc ataatagtta gtgcctgaaa atatcttacc
     1801 gccgaatttg gcatctatca ggaaggaaag aacgaaatgt ttgtatctga attcactgtt
     1861 gatcccggtt gaccagggat cgatgccgga tccgacatcc acataattgg tgcctgcggc
     1921 cggtgcgccg gtcagcggat cgatcacgat attgccctta tcgtcccgtt ccgggtccgc
     1981 gacaaatatc tggtaagccg atttacccag cgcctgcgac atataggcca cgccgattcc
     2041 ctcgtcttcc cccgcgcggg aatagccgag gggataattc gttgacccgg tcgaaagcga
     2101 cagcacctta ttatcattat aggtgaggtt cagggtttca gtccagctga aattttttgc
     2161 tttaaccagc acgccggaca gcaaaagctc gatgccttta ttttctatcg tgcccgagtt
     2221 gagcaaaacg gcatcatatc ccgaggttcc ggaagtaaca gcgggtataa cttccctgct
     2281 ctctttcttg tcgtaataag cagcgtccac gtgaatccgg ttgttaaaga atgtcatctc
     2341 cgtacccact tcaaattctt tggctgaaga gggttccagg gtagaattag gcaccgtacc
     2401 gccgttcgat atattgccga tcgggtatcc gtttaaattc ccgacattat tatagcccag
     2461 cagggtttgg taaggggcat ccgcttcgcc tcccacgttg gcgtaaccga ctcttaattt
     2521 gcccatgtcc atggccggta tatgaaccag ttcggagaaa acaaaggcgc cgctgacgga
     2581 gggataaaaa taatttgtct ttcccggcgc cagggtggag taccagtcat tacgaccggt
     2641 agccgtcagg tacaggtagt ttttgaaaga aaaatcaagc gtgccgtaga tcgatttatt
     2701 ttcgatcacc gggttgctgg tatattctgt aggactggcc aggtttccga tggtatacag
     2761 gtaaggcgtt tgaaaagtat tcccctgcgc gtcaatctga tctaccacgg ccttccgtga
     2821 gttgactccc accagagcgg agagcttgaa gtcttttgca attttaaatt ccttgccgat
     2881 gattccatcc acattaagtt ctgttgattt gatgttctct tcctgcatga acccgtcaag
     2941 ctggtaagcg gtcccgttgg gcgtcacatt ggtatttctg tcggcaaaat aatcttcccc
     3001 tgcccggaat tccaggtaca ggccattgtc aaagctgtat ttaagatcgg ctgaaccgat
     3061 gaaccggttt cgccgggtgt tattaataaa cttgtaggcc gcaaaatagg gattggtcgt
     3121 ataggcatcg tccaaaaact gattttcaga accatcctct ttgtatcccg gagcgaggtc
     3181 ttttatatta acattgggcg gaagaaataa agtggcaaaa ttcacgctgc ccggagcatc
     3241 ggatacactc ggacgattgt gggtaaatgc atttaaatat tgtcccttta actggaggtc
     3301 taaatgcttg tccagtttat aatttacgga taacgaaaag gattgctgct tgaacccggc
     3361 attggggatc aggctggtat tgtccaggtc gctggccgaa aaccgaatgc ttccgtcagt
     3421 gcccaggctt ttactgaaag acaaagtatt cgttgccgcg tcgccatcct tataaaatcg
     3481 gccgatatta ccggaaactg ccgagtaggg ccgctgaacc ccgtcaaatt gataaacctg
     3541 cgatccgtcg agcttcgcgc cccagctgga caatccggtt tcaaaagcat cgtctccgtc
     3601 taccgtctta tttccgtcag atccctggcc ataggttttt tgccagtccg tattgtctat
     3661 tacattttga atgacataat tggaatttaa ttccacgccc gtgcctttgg cgtttttgcc
     3721 ggatttggtg gtgatcacga taacgccctt gcttccacga tatccatata aggcagaagc
     3781 cgcggctcct tttaatacgg taatggtttc tatatcatcc ggattgatgt tgatggtacc
     3841 gtctccgccg tctgctccgc cataacctcc gcccacatcc gtctgccggt aggtgctgtt
     3901 ttgcatgggg ataccgtcga tcacatacaa aggctggttc gaaccggtaa tgtcggaaat
     3961 cccgcggatg accacgtttg ccgaaccgcc gggccctgtg gccacgttgc tgacgttgac
     4021 gcccgctacc tttccttcca ggttgttcac aaaattgacc tccctcgcct ccgtgagggt
     4081 tgagccggcg accgtggtga ccgaatatcc aagggatcgc ttttcccttt taatccccag
     4141 ggccgtaacg accacgtcgg tcaattgggc tgccgactcc tccagtcgca tattgatgac
     4201 cgtttgactg cctaccggaa cttcctcgta tttcctgccc acataagaaa aggtaagggt
     4261 ggccgtggaa ccggctgaaa tggaataatt tccgtcctta tccgtttgta ccagggtggt
     4321 cgttcctttg atacctactg aaacgcctgc cagcgcctca ttggttttta catctgttac
     4381 ccttccctta atgacctgaa cagccggcgg attctgagca acagccatac tgctgaataa
     4441 tagaaaaatt aaagtcttca ggaaaccggc tttaaaaaca aggctacctt tcccggcgtt
     4501 aagattttgg ttcatagcat atagttaaag tgaaaaccac ggttaggttt tatcctagcg
     4561 ttgacagact taaatcgaat ttgttgcaga tgagttggat agccaatgac acacggaact
     4621 ctactcagac aagggtattg attattctgc tgcatgtaat tccggtcagg ccctgtaaaa
     4681 ccaatcactt cttcccagag ccggcgcgcg gacttctatt ccaggtttaa ggatgagata
     4741 ttaaatatac gaaaaagacc aacccatagt cgctaaccga aaataccctc cgct
//
LOCUS       DASYCU010000186         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4101528, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000186 DASYCU010000000
VERSION     DASYCU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4101528"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VGM24_03730"
     CDS             <1..630
                     /locus_tag="VGM24_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4154503.1"
                     /translation="YAVPTVPIISGDDFVSETLLAYELGWRIQPVSRLSLSVAGFFND
                     YDDLRSAEPGPPPSGYPITFGNGVRGDTYGVELSGTYQPADFWRVRGGYTFLKKKLSV
                     KPGSQDLNKAGAESNDPENQFLIQSMFDLPAHIETGFVLRYVDELPNPKVPAYVGLDL
                     QFGWKLTKGLELSITGQNLLRNRHAEFIPTSPSPREILRSVNGKLTCRF"
     gene            618..1184
                     /locus_tag="VGM24_03735"
     CDS             618..1184
                     /locus_tag="VGM24_03735"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY4154504.1"
                     /translation="MPVLKSILIRSFYAALITACVSVQLCAQSAVSREYQIKAAFLFN
                     FTQFVDWPANSFSADDAPIVIAVLGENPFGSYLKETVSGEKVKGHPVVVQYYDHPEEI
                     KTCHILFIGLSDPKKTEQVISALKEKSILTISDMTGFLQEGGMIKFFTRQNNIRFEIN
                     PAAAKAADLTVSSKLLRVAEIFDPSKNK"
     gene            1188..2630
                     /locus_tag="VGM24_03740"
     CDS             1188..2630
                     /locus_tag="VGM24_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4154505.1"
                     /translation="MLFKDIPIRRKLMRGVMLICCMVLIVTCISFFCYELYVFRKSTM
                     EKLSTIGRILASNSTAALAFDNREDAKEILLALRTEPHIVTAALYDKNGNLFSRYPAG
                     LDIHDFPEKPGATGYHLKQAYVLGFQPVVQDKRQLGFLYLNSDLGGMYERFRIFGTVA
                     AFVIILSFLLALLLSRILQKSISNPILALAETARIIHDQQDYSVRAVKIGQDELGTLT
                     DAFNNMLARIEQQNHILKEFNQNLELKVQERTTQLESVNKELEAFSYSISHDLRAPLR
                     AIIGFTAILEEDYSSKLDGEAKRITSVIRKNTLKMGHLIDDLLTFSRMGRQDIVKTNM
                     LTGLMVEEVINELVPENKIGKISWIIHEVPDIKGDINAMRQVWINLISNSIKYAGNRD
                     QPRVEIGSYPERGQTVFFVKDNGVGFDEKYKDKLFKVFQRLHSADEFEGTGVGLAIVE
                     KIVSKHGGLVWAEGQVNKGASFYFSLPAEF"
     gene            2671..3114
                     /locus_tag="VGM24_03745"
     CDS             2671..3114
                     /locus_tag="VGM24_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4154506.1"
                     /translation="MNHNSVEVLLVEDNLNDAELTIRELRKHHMANNLFHVKDGEEAL
                     DFIFGLGKYADSRDLLYRPKVVLLDIQMPKVNGIEVLQKVKADPRTKATPVVILTSSK
                     EDPDIQKCYDLGANSYIVKPVNFESFAQAIKNLGFYWLLLNQQPT"
     gene            3121..>3493
                     /locus_tag="VGM24_03750"
     CDS             3121..>3493
                     /locus_tag="VGM24_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4154507.1"
                     /translation="MKAALKIIMLEDSAVDAEIIQRFLAKKKPDYQFCLVTNKEAFLQ
                     ALDQFNADLVLADNALPQFNGSEALQIVRETALDMPFILVTGSMTDEFAAHIIKSGAD
                     DYILKDRLTRLPDAIEAAIKKR"
BASE COUNT          947 a          791 c          893 g          862 t
ORIGIN      
        1 tatgcggtcc cgacggttcc gatcatatcc ggtgatgatt tcgtttcaga aaccctgctg
       61 gcatacgaac tgggatggag gattcaaccg gtcagcagac tttccctctc cgttgccgga
      121 ttctttaatg actatgatga tctccgcagc gcagagcccg gtccgccgcc ctccggctat
      181 cccatcactt ttggaaatgg agtccgggga gatacctatg gtgttgaact ttccggcacc
      241 tatcagccgg ctgatttctg gcgcgtccgg ggcggataca cttttttgaa aaagaagctc
      301 tcggttaagc ccggaagcca ggatctcaat aaagcaggag ccgaaagcaa cgatccggaa
      361 aaccagtttc tgatccagtc catgtttgat cttccggcgc atatcgaaac ggggtttgtg
      421 ctccggtatg tggatgaatt gcccaatccg aaagttcccg cttatgtggg gctggattta
      481 cagtttggat ggaaactgac aaaagggctt gagctgagta ttaccggaca gaatctactg
      541 cgtaacagac acgctgaatt catacccaca tcaccgtctc cgcgagaaat attgcgtagc
      601 gttaatggaa agctgacatg ccggttttaa aatcaatcct tatccgttcc ttttatgccg
      661 cactgattac agcgtgcgtc tccgtgcagc tgtgtgccca gtcagccgtt tccagggaat
      721 accagataaa ggcggctttt cttttcaact tcacccagtt tgtcgactgg ccggcgaatt
      781 ctttttccgc agacgatgcg ccgatcgtca ttgccgttct gggagaaaat ccctttggtt
      841 cctatctgaa agaaacggta tcgggcgaaa aagtgaaagg ccatccggtg gttgttcaat
      901 attatgatca tccggaagag atcaaaacat gccatatcct gtttatcggt ctttctgatc
      961 ccaaaaaaac ggaacaggtt atttccgcct taaaagaaaa gagcattctg acgataagcg
     1021 atatgaccgg atttttacag gagggcggga tgatcaaatt ctttacccga caaaataata
     1081 taaggtttga aataaatccg gcagcggcca aagcagcgga tctgaccgtg agttcaaagc
     1141 tgctccgggt ggcggaaatt tttgatcctt caaaaaacaa atgagccatg ctattcaaag
     1201 acattccgat acgcagaaaa ctgatgaggg gggtcatgct gatctgttgt atggttttga
     1261 tcgtgacctg cattagtttt ttttgttatg aactgtatgt cttccggaaa tcaacgatgg
     1321 aaaagctctc taccatcgga agaatccttg cttcaaacag cacggctgcg ctggcgtttg
     1381 ataaccggga agacgcaaaa gagatcctgt tggcccttag aaccgaaccc catattgtaa
     1441 ccgctgccct gtacgataag aacgggaatc tcttttccag gtacccggcc ggcctggaca
     1501 tccatgattt tccggaaaag cccggagcta cgggatatca tttgaagcag gcatacgtat
     1561 tggggtttca gccggtggtc caggataaaa ggcaactcgg tttcctctac ctgaattctg
     1621 atctcggcgg catgtatgaa cgattccgga tcttcggaac ggtggcggca ttcgtaataa
     1681 tcctttcttt cctgctggca ctgcttcttt ccaggatcct tcagaagagt atttcaaatc
     1741 cgatccttgc cctggccgaa acggccagaa taattcatga tcagcaggac tattcggtgc
     1801 gggctgtcaa gataggccag gatgaattgg gcacgctcac cgatgccttc aataatatgc
     1861 ttgcccgcat tgagcagcaa aatcatatct tgaaagaatt caaccagaac ctggagctaa
     1921 aggttcagga gcgtaccacc cagctggaat cggtgaataa ggaactggaa gccttttctt
     1981 attctatttc acacgatctg cgggcgcccc ttcgggccat cattggtttt acagcgatcc
     2041 tggaagagga ttacagcagc aagctggacg gagaagccaa acggattacc tccgtcatca
     2101 gaaaaaatac gctgaagatg ggccacctga tcgatgacct gctgacgttt tcgaggatgg
     2161 ggcggcagga cattgtcaaa acgaatatgc tcaccggtct catggttgaa gaagtgatca
     2221 atgagttggt tccggaaaat aagatcggaa agatcagctg gatcattcac gaggtgccgg
     2281 atataaaagg agatatcaat gcaatgcggc aggtatggat aaacctgatc tccaattcca
     2341 taaaatatgc aggaaaccgg gaccagccgc gggtggagat cggatcttat ccggagcgcg
     2401 gacaaacggt cttttttgta aaagataacg gggtggggtt tgatgaaaag tataaggaca
     2461 aattatttaa ggtgtttcag cgactgcata gtgcggatga atttgaaggg acgggcgtgg
     2521 ggctggccat tgttgaaaaa attgtttcca agcacggggg gctcgtgtgg gcggaagggc
     2581 aggtgaacaa gggagcgtct ttttatttca gcctgcccgc cgaattttaa aaagttcagg
     2641 gtaaaaaaaa ttattcaaac cctttaaaat atgaatcata attctgtgga ggtattactg
     2701 gtggaagaca atctcaatga cgcggagctc accatccggg aattgagaaa gcaccatatg
     2761 gcaaacaacc tgtttcatgt taaagacgga gaagaggcgc tggattttat tttcggtctg
     2821 ggtaaatatg cagattccag ggatttgctc taccgtccga aagtggtatt gctcgacatc
     2881 cagatgccca aagtgaacgg gatcgaagtg ttgcagaagg tgaaagcgga cccccgcacc
     2941 aaggccacgc cggtggtgat cctgacttca tcgaaggagg acccggatat tcagaaatgt
     3001 tatgacctcg gagccaacag ttatatcgtg aagccggtaa attttgaaag ctttgcccag
     3061 gccataaaaa accttgggtt ctactggtta ctattaaacc aacaacccac ctgattgaat
     3121 atgaaagctg cactaaaaat aatcatgctc gaagacagcg cggtagatgc ggaaatcatt
     3181 cagcggtttc tggcaaagaa aaaaccggat taccaattct gcctcgtaac caataaggag
     3241 gcctttctcc aggccctgga tcagtttaac gctgacctgg tgctggctga taatgcgttg
     3301 ccgcagttca acggttccga agccctgcag atcgttcggg aaacggcgct ggatatgcct
     3361 tttatcctgg taaccggcag tatgacggac gagtttgctg ctcatattat taaatccggg
     3421 gcagatgatt acatcctgaa agaccggctc acccggctgc cggatgcgat tgaagcggcg
     3481 ataaaaaaaa gaa
//
LOCUS       DASYCU010000187         4228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3523344, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000187 DASYCU010000000
VERSION     DASYCU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3523344"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1269
                     /gene="pdhA"
                     /locus_tag="VGM24_03755"
     CDS             274..1269
                     /gene="pdhA"
                     /locus_tag="VGM24_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY4154508.1"
                     /translation="MATKFSKETYLYWYELMQLIRQFELMAEEKYKMEGKIRGFFHAY
                     IGQEAIAAGCMTASRPDDPFITGYRDHGLALSKGVSANACMAELYGKSTGAAKGKGGS
                     MHFFGVGVKFFGGHGIVAAQIGTGAGLAFAEKYKGTDNAVLTFFGDGAARQGMLHEIF
                     NMAMLWKLPVVFICENNNYAMGTSVERTSNVVDIYKLADAYEMPGDSIDGMNAEAVHE
                     GVSRAIKRAREGGGPTLLEIKTYRYKGHSISDPAKYRTKEEVEEYKQRDPIAQVTDSI
                     LTRKFATKEELEAINKRVADTVAAAVKFAEESPIPNDDEVYKDIYIDKNYPFIKD"
     gene            1310..1990
                     /locus_tag="VGM24_03760"
     CDS             1310..1990
                     /locus_tag="VGM24_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4154509.1"
                     /translation="MSEIKHPEHLKHKKTAAENWMLFQDRYGKQALIGVVVLLLVAGV
                     YFGYRHFVQEPKEKQAEEAMFRAEDYFRMDSIKLALSGDNVNAGFTKVISRYPGTAAA
                     SLASFYAGSCYLKLGDFNNAVKYLKDFRTPALQVQAKAYGLLGDAYSELNKKEEAVAA
                     YKKAGTTFEKDDIISPEYLFRAGYLYESMGRNREAIEMYQLVKDKYPGSQRGFDIDRY
                     LARLGVVK"
     gene            1997..2494
                     /gene="ribH"
                     /locus_tag="VGM24_03765"
     CDS             1997..2494
                     /gene="ribH"
                     /locus_tag="VGM24_03765"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY4154510.1"
                     /translation="MASSGKSKTAAPKIRKASVVLVRTEWNAQVVDELYSGCKKKLFA
                     EKANLVGDILVPGAFEIPFAIKKYWEETKHSKTGRPEAFIALGCIIRGDTPHFEYVCR
                     AVTDGIVRLNLALPVPVIFGVLTVDNLEQARERTGGLHGHKGEEAAETALKMISFCRN
                     PGNLK"
     gene            2503..3222
                     /locus_tag="VGM24_03770"
     CDS             2503..3222
                     /locus_tag="VGM24_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY4154511.1"
                     /translation="MKVQIFIPCFIDQLYPETAFNMVRVLEKAGCEVVYNTEQTCCGQ
                     PAFNAGFRDDAREVCIKFLEDFSGTDYIVSPSASCTGFVRNYYPLLFPDSAHLKEVKQ
                     TGKRIYEFSDFLVNVLKMDDFQAVLWGKATYHDSCAGLRECRIKAEPRRLLSKVKGLD
                     LVEMKDVETCCGFGGTFAVKFESISMAMADQKVHHALETGADYLISTDMSCLMHLDGH
                     IRHKGLPIKTMHIADVLASGW"
     gene            3565..3975
                     /locus_tag="VGM24_03775"
     CDS             3565..3975
                     /locus_tag="VGM24_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY4154512.1"
                     /translation="MAHWLIKSEPEVYSWDQLVKDKQTRWDGVRNYAARIHLRAMKNK
                     DQVFFYHSNKGMEIVGIGTVSREAYPDPTTDDPAWVAVDIKPLRKLLKPVTLDQIKKE
                     KKLANMALVRLGRLSVQPVTDEEWQMVMKMGGME"
     gene            3975..>4228
                     /locus_tag="VGM24_03780"
     CDS             3975..>4228
                     /locus_tag="VGM24_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154513.1"
                     /translation="MVNRQFKKTGISFIVFLLLAGVLPAQSGTELKNLKTFVREANPP
                     VYDTSLEFITDRIGDNLHYLYPLYQLLGSEKKFRERFSDA"
BASE COUNT         1186 a          992 c         1044 g         1006 t
ORIGIN      
        1 agaattcaaa tccaaaggaa ctccggttta tttccgatgt tccggtgccg gccgatttgg
       61 caaaccggtt gattgaatcg gtggatttgc aggataccgc aaacagaaat actgcgatga
      121 gcgccttttt catagaaaat agtcaggttt aagcagttag aaagaataaa aacagttcgc
      181 aaaactcgtc cttagatcca aaatttcctg tttacccctt atctttgccc cgcctttgcc
      241 gatccccggc aggttaaaat caaatttacg caagtggcca ctaagttttc aaaagaaacc
      301 tacttatact ggtacgagct gatgcagctg atccgtcagt ttgagctgat ggcggaagaa
      361 aaatataaaa tggaaggtaa gatccgcggt tttttccatg cctatatcgg gcaggaggcc
      421 attgctgcgg gctgcatgac cgcttcgcgt ccggatgatc cattcattac cggctaccgg
      481 gaccatggcc tggccctttc caagggggtt agcgccaatg cctgtatggc cgagctatac
      541 ggaaaatcaa ccggtgccgc caaaggaaag ggcggaagca tgcacttttt tggcgtgggc
      601 gtaaaatttt ttggcggcca tggtattgtg gccgcacaga tcggaaccgg cgccggtctt
      661 gcttttgccg aaaaatacaa gggaaccgac aatgccgtgc tcaccttttt cggagacgga
      721 gcggcgcgtc agggaatgct ccatgagatc ttcaatatgg ccatgctctg gaaacttccg
      781 gttgttttca tttgtgaaaa taacaactac gccatgggta cttctgtgga aagaacctcc
      841 aacgtagtgg acatatacaa actggcagac gcttatgaaa tgccgggaga ttcgatagac
      901 ggtatgaatg cggaagcggt acacgagggc gtttccaggg caatcaagcg ggcccgcgaa
      961 gggggaggac ccaccctgct ggaaatcaaa acctatcgct ataaagggca ttccatttcc
     1021 gatccggcaa aataccggac caaggaagaa gtggaagaat ataaacaaag ggatccgatc
     1081 gcacaggtta ccgacagcat tctgacgcgg aaatttgcca caaaagaaga actcgaagcc
     1141 atcaacaaac gggtcgcgga taccgttgct gctgcggtaa aatttgcgga agaatccccc
     1201 atccccaacg atgacgaggt ctataaagac atttatattg ataaaaatta cccgttcatt
     1261 aaagactgat catttaatcc aaaacccggt tttacattaa ttcccagaca tgagtgaaat
     1321 caaacatccc gagcatctta aacacaaaaa aacagccgcc gaaaactgga tgctttttca
     1381 ggaccggtat ggaaaacagg ccctgatcgg cgtggtcgtt ctgctgctgg tggccggagt
     1441 ctatttcgga taccggcatt ttgttcagga accaaaagaa aagcaggcag aagaagcgat
     1501 gttccgggcc gaagattatt tccgcatgga ttctataaaa ctggccctga gcggcgacaa
     1561 cgtaaatgcc ggatttacga aggtcatttc caggtacccg ggcaccgcgg cggcgtcact
     1621 ggcttctttt tatgccggca gctgttacct gaaactcggc gattttaaca atgcggttaa
     1681 atacctgaaa gacttccgca ccccggcgct ccaggttcag gcaaaagcat acgggctcct
     1741 cggggatgcc tattccgaat taaataaaaa ggaagaagcc gttgcggcct ataaaaaagc
     1801 aggaacaaca tttgagaaag acgatattat ttctcctgaa tatcttttcc gggccggata
     1861 cctttacgaa agcatgggca ggaaccggga ggccattgaa atgtaccagt tggtaaaaga
     1921 caaatatccc ggatcccaga gaggatttga tatcgatcgc tatctggcca gactgggtgt
     1981 tgttaaataa ctgcgcatgg catcttccgg aaaatcaaaa acggctgctc caaaaatcag
     2041 aaaagcttcg gtggttttgg taagaacaga atggaatgcg caggtcgtag acgaactcta
     2101 ctcgggttgc aaaaaaaagc tgtttgcaga aaaagcaaac ctggtcggag atatcctggt
     2161 tcccggcgcc tttgaaattc cctttgcaat aaaaaaatat tgggaagaaa caaaacactc
     2221 caaaaccggc cggccggagg cttttatagc cctgggttgc atcatccgcg gagatacccc
     2281 tcattttgaa tacgtctgcc gggcggtgac cgacggcatt gtccgcctga acctagccct
     2341 tccggtaccg gtgatttttg gtgtccttac ggtagacaac ctggagcagg cccgggaaag
     2401 aaccggcggt ttgcatggtc ataaaggaga ggaagccgca gaaaccgcat tgaaaatgat
     2461 ttcgttttgc cggaatcccg gcaatcttaa ataattccga tattgaaagt ccagattttc
     2521 ataccgtgct tcatagatca gctttatcct gaaacggcgt tcaatatggt ccgggtgctt
     2581 gaaaaggcgg ggtgcgaggt tgtttataat acagaacaga cctgctgcgg gcaacccgca
     2641 ttcaatgccg gattccggga tgatgcgcgg gaagtctgca tcaaattcct cgaagacttc
     2701 agcggaaccg attatattgt atcccccagc gcgtcctgta cgggttttgt aagaaattat
     2761 taccccctct tattcccgga ttcagctcat ttgaaggaag taaaacaaac gggcaaacgc
     2821 atttatgaat tttctgattt cctggtcaat gttctgaaaa tggatgattt ccaggctgta
     2881 ttgtggggca aggccactta ccacgattcc tgcgcaggac taagggaatg ccggatcaag
     2941 gccgaacccc gccgtttatt atcaaaagta aaagggctcg acctggtgga aatgaaagac
     3001 gtggaaacct gttgcggatt tggcgggacc tttgccgtca aatttgaatc catctccatg
     3061 gccatggccg accagaaagt tcatcatgcc ctcgaaaccg gagcagatta cctgatatcc
     3121 acggatatgt cctgcctgat gcacctggac ggtcatatcc gccataaagg acttcccata
     3181 aagaccatgc acatcgccga tgtgctggcc agcggctggt aaaaaaaata gaccatcccc
     3241 catgccagaa ggcaggcaga tggtctgacc ttaaaacaac ctgtcgcaaa tatttaagcc
     3301 ggtaataagc gacacttaat actgtatacg taaagatttc aaaaaggatg tttaggttct
     3361 cttctttttt ttggtcttcg tttccggcca gtctcccgga tcagcctctt ttccggtatt
     3421 ccatcagccg gcggatgtat ccacctgccg cttttgccgg atttagcatt ttctgaaaaa
     3481 tttacaagat ggggccggat gcgtatccgg tgcgaaccta cctttgaccg gaaaaccaat
     3541 tcatccctgt ttatctgttt ttttatggca cactggctta ttaaatcgga acccgaggtt
     3601 tattcctggg accagcttgt aaaagacaaa caaacccgct gggacggcgt acggaattat
     3661 gctgcccgca ttcacctcag ggccatgaaa aataaagacc aggtattttt ttaccatagt
     3721 aataaaggca tggagattgt tggcattggg acggtgagcc gggaagccta cccggatccg
     3781 accaccgacg atccggcctg ggttgcagtg gatataaaac cccttagaaa attgcttaag
     3841 ccggtgacgc tcgatcagat taaaaaagaa aagaaactgg caaatatggc actggtcagg
     3901 ctggggcgct tatctgttca acccgtaacg gatgaagaat ggcaaatggt catgaagatg
     3961 ggagggatgg aataatggta aacaggcaat tcaaaaagac cggaataagt ttcatcgttt
     4021 tccttttgct ggccggcgtc ctgcccgcac agtccggtac ggaactgaaa aatctgaaaa
     4081 cctttgtcag ggaggccaac cccccggttt acgacacttc ccttgaattt attacagacc
     4141 ggatcggaga taatctgcat tatctctacc cattgtatca actgctgggt tccgaaaaaa
     4201 aattcaggga aagattttcc gacgcgag
//
LOCUS       DASYCU010000188         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1493339, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000188 DASYCU010000000
VERSION     DASYCU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493339"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="fmt"
                     /locus_tag="VGM24_03785"
     CDS             <1..905
                     /gene="fmt"
                     /locus_tag="VGM24_03785"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY4154514.1"
                     /translation="PDFAVASLEAILQNGYPVVAVVTAPDKPAGRGMKLLESEVKQFA
                     RKRGIPVLQPDKLKDPGFIEQLKRFEADIQVVVAFRMLPEIVWNMPSMGTVNLHGSLL
                     PQYRGAAPINRAIMNGETETGLTTFKMKQEIDTGNILLMEKIAVGPTETAGELHDRMK
                     ITGGNLLVKTIRGLLDGTIKERPQPVPEGGLIKTAPKIFTADCEIDWTQPVSKIQNQI
                     RGLSPYPGAFTRFSDKIFKIFRSYPEISSDHPAPGSYETNGRSWLRFSAIDGYIYTEE
                     IQPEGKKRMPVTDFLRGYRGETIR"
     gene            complement(948..2411)
                     /locus_tag="VGM24_03790"
     CDS             complement(948..2411)
                     /locus_tag="VGM24_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEY4154515.1"
                     /translation="MKAFTLLLIFISSTAFLLARCAKKNPDKAASGDTTTTVPPMVKN
                     EVDFWLTTGDQATLLTKQPSVLAFGTKANSYPDIEVDSSSVLQPIDGFGYTLTGGSAS
                     LINKMGGDAKSALLLELFGNKENSISLSYLRISIGASDLNAAPFTYDDMPEGQTDTSL
                     LHFNLDTDQTAGTGLIPLLREIIAIQPTIKIIATPWTAPVWMKDNDNFKGGSLQAKYD
                     AVYASYFVKYLQAMKTSGIQIDAITPQNEPLNPDNNPSLFMESGQQADFIKNYLGPAF
                     RAAGLTTKIIIYDHNCDKPDYPLAILNDADALPYINGSAFHLYAGDISALTTVHNAFP
                     DKAVYFTEQYTASTGAFAGDLSWHLKNVIIGSMRNWSRNALEWNLANDPDYGPHTEGG
                     CNSCKGALTIDGASIKRNVAYYIIAHASKFVPPGSVRISSNITGNLYTVAFLRPDGKK
                     VLIVLNDNSVTTAFNIRFKNKWVSPQLAGGSVASFIW"
     gene            2668..3141
                     /locus_tag="VGM24_03795"
     CDS             2668..3141
                     /locus_tag="VGM24_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY4154516.1"
                     /translation="MKIEFWSLGKPHEPYIKKGVDDFTSRIQNYYPVEWRIIAAGKKA
                     GARLPGKPDQPESGLVLASLKPGDYLVALDENGTAFSSAKLARFIEDRGNESVKRLIF
                     LIGGAYGLDKKILSTAKFSWSLSALTFPHQLVRLILAEQIYRACSIIRNEKYHHG"
     gene            3194..>3423
                     /locus_tag="VGM24_03800"
     CDS             3194..>3423
                     /locus_tag="VGM24_03800"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY4154517.1"
                     /translation="MFTITLSIILITSAISITGFRNGKVVDDLIFWPPAITKKYQYYR
                     FITCGLIHADYMHLIFNMLTLYFFGRIMEVYY"
BASE COUNT          886 a          807 c          869 g          861 t
ORIGIN      
        1 cgcctgattt tgccgtcgct tccctggagg ctatccttca aaacggatac ccggtcgtgg
       61 cggtggtcac tgcacccgac aagccggccg gacggggtat gaaactcctg gagtctgagg
      121 taaaacaatt tgcccggaaa aggggtatac cggttttaca gccggataaa ctgaaggatc
      181 ccgggtttat tgagcagttg aagcgttttg aagcagacat ccaggtggtc gttgcttttc
      241 gtatgcttcc ggaaattgta tggaatatgc cttcgatggg aacggtcaac ctgcacggtt
      301 ccctcctgcc gcagtaccgg ggtgcggccc ccatcaaccg cgcgataatg aatggtgaaa
      361 cggaaaccgg gctaaccact tttaaaatga agcaggaaat tgataccggt aatattttgc
      421 tgatggaaaa aatagctgtc ggtccaacgg aaacggcagg tgaattgcac gaccggatga
      481 aaataaccgg tgggaacctg ctggtaaaaa cgattcgtgg attgctggat ggtaccataa
      541 aggaaaggcc gcagcctgtt ccggagggcg ggctgataaa aacggctccc aaaattttca
      601 cggccgattg cgagatcgac tggacacagc cggtttccaa aatacaaaat cagatccgcg
      661 gcctctcccc ttatcccggc gctttcaccc ggttcagcga taaaatattt aagatattcc
      721 ggtcctatcc ggaaatcagt tcagatcatc cggcgcccgg gtcttatgaa acaaatggca
      781 gatcctggtt acgtttcagc gccatcgatg ggtatatcta cacggaggaa attcaacccg
      841 aagggaaaaa aaggatgccg gtcacagact ttctccgggg ttaccgggga gaaacaatcc
      901 ggtaaatttc aattccttta cccggtgcat tagtaatccg gtattcatca ccagataaag
      961 ctggccacgc tgcccccggc cagctgcggg gaaacccatt tattcttaaa gcggatatta
     1021 aaagcggtgg tcactgaatt gtcgttgagg acaataagaa cttttttccc atccggtctc
     1081 agaaaggcaa cggtatacag gttaccggta atattactgc tgatccggac cgaaccggga
     1141 ggaacaaatt tggacgcatg cgcaatgata taataagcta cgttccgttt tatggatgct
     1201 ccgtcgatcg tcagcgctcc tttgcaggaa ttgcatcctc cttccgtatg cgggccgtaa
     1261 tccggatcgt tggcgaggtt ccattccagg gcattccggc tccaattgcg catggaacca
     1321 atgatcacat tcttcagatg ccaggataaa tctccggcaa aggccccggt tgaagcagtg
     1381 tactgctccg taaaataaac cgctttatcc gggaaagcat tgtgaacggt ggtcagcgca
     1441 ctgatatctc cggcatataa atgaaaagca gaaccattta tataaggaag ggcatccgca
     1501 tcatttaata tggccagcgg ataatccggc ttatcgcaat tgtggtcgta gataatgatt
     1561 tttgttgtta gtcccgctgc ccggaaggca ggtcccaggt aatttttaat gaaatcagcc
     1621 tgctggccgg attccatgaa caaactgggg ttattatcgg gattcagggg ttcattttgt
     1681 ggcgtaatgg catctatctg aattccggaa gtcttcattg cctgcaggta tttcacaaaa
     1741 taggaggcgt aaacggcatc atattttgcc tgcagactgc ctcccttaaa attatcatta
     1801 tccttcatcc agaccggcgc agtccagggg gtggcgataa ttttaatcgt gggttgaata
     1861 gcgattatct cgcgcagcag cggaatcagc cctgtgccgg ccgtctgatc tgtgtccaaa
     1921 ttaaaatgca gaagggaagt atcggtttgc ccctccggca tgtcgtcata ggtaaatggc
     1981 gcagcattca ggtcggaagc cccgatgctg atccggagat agctcaggct gatggaattt
     2041 tccttatttc caaaaagttc cagcagcagg gcggatttgg catccccgcc catcttattg
     2101 atgaggctgg cacttcctcc cgtcaaggta taaccgaatc cgtcgatggg ttgtaaaaca
     2161 gaactgctgt ccacttcaat atccgggtag ctgttggcct ttgtgccgaa ggcaagaacg
     2221 gaaggctgtt tggtcagcag ggtcgcctga tcgccggtgg taagccagaa atccacttca
     2281 tttttaacca tgggcgggac agtggtcgtg gtatctccgg aggcagcttt atccggattt
     2341 ttttttgcac agcgggccag cagaaaagca gtggaggaaa taaaaatcag taaaagggta
     2401 aacgctttca tcccggtttt tttcaatttt ccagtgcctg ctatccggca gccatctgtt
     2461 ggcggaccgg tcacccattt aggatacgaa tcacggtccc gcagaaatta aaaataaggg
     2521 ttctggctga aatccaaagg caatctttta cccgcggcct aagaagggca gaaggaaggg
     2581 ccttccactg atggattttt gcaggaatcc tgggaatcaa tattgaaaac cggagaatga
     2641 atagccggat cgggttaatt ttaaattatg aaaatagaat tctggtcgtt ggggaaaccc
     2701 catgaaccgt atattaagaa aggcgtggat gattttacct cgcggattca gaattactat
     2761 cccgtggaat ggcggatcat cgcagccggg aaaaaggctg gcgccaggct gccgggaaaa
     2821 ccggatcaac cggaatccgg actggtcctt gcttccctga aaccgggtga ttatctggtt
     2881 gccctggatg aaaacggaac ggccttctct tcggcgaaac tcgcgcggtt cattgaagac
     2941 cggggaaatg aaagtgtaaa aagattgatc tttctgatcg gcggggcata cgggctggac
     3001 aaaaaaatcc tttccacggc aaaattcagc tggtcacttt ccgcacttac ttttccacat
     3061 cagctggtgc ggttgatcct ggccgaacag atctatcgtg cctgcagcat catccggaat
     3121 gagaaatacc atcacggctg aaccgttttc catttggaat taaaccatat ctttaaaccc
     3181 tttatcaaac ggcatgttta ccattacgct tagtattatt cttattactt ccgccatttc
     3241 cataaccggt ttcaggaatg gcaaagtggt ggacgatctt attttctggc ctccggcgat
     3301 taccaaaaag taccagtatt accggtttat cacctgtggg ctcattcatg ccgactacat
     3361 gcacctgatc ttcaatatgc tgaccctgta ttttttcgga aggatcatgg aggtctacta
     3421 tat
//
LOCUS       DASYCU010000189         6002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1202087, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000189 DASYCU010000000
VERSION     DASYCU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202087"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGM24_03805"
     CDS             <1..283
                     /locus_tag="VGM24_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HEY4154518.1"
                     /translation="TMDIWTSSSVPGASVFKSLLSLNPSQAGMFTALEKAGCGGVLVK
                     ISMSGKDYAFDENLIQAEKRNYSADLFKIPNGYSESTGNMFSHMMQPEK"
     gene            320..757
                     /locus_tag="VGM24_03810"
     CDS             320..757
                     /locus_tag="VGM24_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154519.1"
                     /translation="MKKIFKTTFIAALVLLTAASCKKSTAKKSSGTSAIEGFWTYKED
                     PALDYWNDNVLFKSDGTFRMYTALSLADTSAAQALADTASQVVTFGTYTVNGNDVEMV
                     WEELSVVGFTFSGTLNGSKNNIIGNIETKEPDSASPLWYLTKP"
     gene            956..3232
                     /gene="katG"
                     /locus_tag="VGM24_03815"
     CDS             956..3232
                     /gene="katG"
                     /locus_tag="VGM24_03815"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEY4154520.1"
                     /translation="MEKESSNISKCPFHNGSVKHNVGGGGTANRDWWPNRLKLNILRQ
                     HSSRSNPMGEDFNYAEAFKSLDLEAVKKDLHALMTDSQDWWPADFGHYGPFFIRMAWH
                     SAGTYRVGDGRGGAGQGQQRFAPLNSWPDNVSLDKARRLLWPVKQKYGRNLSWADLLV
                     LAGNIALESMGFKTFGFGGGRPDVWEPDEDVYWGAETTWLGGDLRYAQGSPGVEEDHG
                     VLVSDDDTGGDLHSRNLEKPLAAVQMGLIYVNPEGPDGNPDPIVAAKDIRDTFGRMAM
                     NDEETVALIAGGHTFGKTHGAAPATHVGKEPESAGMEMQGLGWHNDYRSGKGADTITS
                     GLEVTWSKTPTQWSNSYFENLFGFEWELTKSPAGAQQWVAKNASSVIPDAHDRSKKHL
                     PTMLTTDISLRVDPVYEKISRHFLENPEAFADAFARAWFKLTHRDMGPRVRYLGPDVP
                     EEELLWQDPVPAVDHPLVDEKDISALKSKVLESGLSVSELVATAWASASSFRGSDKRG
                     GANGARIRLAPQKYWRMNNPAQLQKVLNVLERIQKEFNAAQAGGKKVSIADLIVLAGG
                     AGVEKAAKDAGQAIAVPFTAGRTDAVQEQTDVESIGFLETPGDGFRNYRKSKFPVSTE
                     ELLIDKAQLLTLTAPELTVLVGGMRVLNTNFDGSNDGVFTLRPGQLTNDFFVNLLDMR
                     TQWKAVSADQELFEGSDRSSGKVKWTGTRADLVFGSNAELRAVAEVYASADAQKKFVQ
                     DFVAAWNKVMNLDRFELA"
     gene            complement(3281..5659)
                     /locus_tag="VGM24_03820"
     CDS             complement(3281..5659)
                     /locus_tag="VGM24_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154521.1"
                     /translation="MIRNFLKIAYRNLLRSKGFSAINITGLAVGMAAAILILLWIRNE
                     ISYDQFHQNKERIYEVWNRVPMQGTIRSWNSVSAPLARVLEKDLPEVERAVRVVNTSS
                     FLFSVGDKKMLKSGNMVDTGFLQMFSFPMLQGNPATALNNIHGMVLTEKAAKSLFGKE
                     DAMGRLVRLKDQGDFTITGVLKDLPNNTRFDFEFLISIANLKYGVGQDLGWNDNSTPT
                     YVLLKPKSSFAAVESRIKTLKQKYSDGAKNMKWELFLYPLDRWRLYSSFTDGVEDSGG
                     RSTFVKLFSIIAAFILLIACINFMNLSTARSEKRAKEVGIRKVVGARKGSLIAQFIGE
                     SVFLTLLAGIIAVIIVQFSLPAYNRLTEKELFIDFGNAYTWMAFTGFILFTGLLAGSY
                     PAFFLSSFRPVKVLKGSFKKANALVTPRKVLVVLQFTFAIILIICTIIVKQQIDYARN
                     RETGYDKDNLIYQFMTGDIPKNYTLIKNELLSSGIAKSVTKTNSPLTERWSDGWGQAW
                     EGKDPNDQTSFDRYLEDEGLGVTAGLQFVQGRDFDLKQYPTDSTGLIINESALKVMKF
                     KNPIGKIVGDLGIEWHIVGVIKDFILTSPYEPTRPILICGAKSQFMTFNVMQIKLNGN
                     NGTAQNLKRAEAIFKKYNPEYPFDARFVDEAYAKKFENEKRQGTLAGLFAGLTVFISC
                     LGLFGLATYMAENRVKEIGIRKILGASLTGITTLLSKDFVKLVVISFLIAAPLAWWGM
                     YTWLQNYTYRVSVEWWVFALAGLLSVLIALLTVSYQSIKAALANPVKNLRTE"
     gene            complement(5711..>6002)
                     /locus_tag="VGM24_03825"
     CDS             complement(5711..>6002)
                     /locus_tag="VGM24_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4154522.1"
                     /translation="KEVGIRKVLGATAGSIVYLFSKEFILLIAIAFAVAAPIAWYYMH
                     QWLQDYAYRINISWWLFAAGGLAAIIIALLTISFQAIKAAIANPVESLRTEG"
BASE COUNT         1483 a         1575 c         1509 g         1435 t
ORIGIN      
        1 caccatggat atctggacaa gcagttccgt gccgggtgct tccgttttta aaagcctgct
       61 cagcctgaat ccgtcccagg ccgggatgtt cactgcactg gaaaaagcag gttgcggagg
      121 cgtgctggtg aaaatcagca tgtccggcaa agactatgct tttgatgaaa accttatcca
      181 ggctgaaaaa agaaactatt cagccgatct gtttaaaatc cccaacggct actcggaatc
      241 aacgggtaat atgttttcac atatgatgca acctgaaaaa taattacccg cttcttttaa
      301 ccttaataga aacaagatca tgaaaaagat attcaaaaca acgtttattg cggccctcgt
      361 gctgctcacg gccgcctcct gtaaaaagtc aaccgccaaa aaaagttccg gcacttcggc
      421 catcgaaggt ttctggacat acaaggaaga tccggcgctc gattactgga atgacaatgt
      481 gcttttcaag agcgatggta cattccggat gtacacggcc ttatcgctcg cggatacctc
      541 cgcggcacag gcccttgccg atacagccag ccaggtagtg accttcggca cgtataccgt
      601 taacggaaac gacgtcgaaa tggtttggga ggagttgagt gtcgtcggct tcacgttttc
      661 cggtaccctg aacggcagca agaacaatat catcggtaat atcgagacca aagaacctga
      721 cagcgcttcc ccgctgtggt atctgaccaa accctaaacc cctttttcat ttttccttct
      781 cctttaaaga aaattcagag tcgccagctc tgaattttcc ctttttccct aatagatttt
      841 gattattgaa ttatagaatt aatcgattgt ggctatcccc ccgatccgct aattttatgc
      901 ccctgctcaa aataccagga aggccggcat tctttcaata cttaaatctt gcacaatgga
      961 aaaagaatca agcaatatca gcaaatgccc ttttcataat ggcagtgtga agcacaatgt
     1021 gggcggtggc ggcaccgcga accgggactg gtggcccaac cggctcaagc tgaacatcct
     1081 tcgccagcat tcatcccggt caaacccgat gggagaagat tttaactatg ccgaagcgtt
     1141 taaaagcctt gacctggagg cagtgaaaaa agatcttcat gcactcatga cggattccca
     1201 ggactggtgg ccggccgact tcggccatta cggccccttc ttcattcgta tggcctggca
     1261 cagtgcaggc acctaccgcg tgggggatgg ccgtggcggt gcgggccagg ggcagcaacg
     1321 ttttgctccg ctgaacagct ggcctgataa cgtgagtctc gataaagcac gcaggctgct
     1381 ctggccggtg aaacaaaaat acgggcgcaa tctttcctgg gcagacctgc tggtccttgc
     1441 cgggaacatc gcgctggaat ccatgggttt taaaacattt ggctttggcg gcggacgccc
     1501 ggatgtatgg gaaccggatg aagatgtata ctggggcgcc gaaaccacct ggctgggcgg
     1561 cgatctgcgt tatgcgcagg gttccccggg tgtggaagaa gatcatggcg tactggtgtc
     1621 ggacgacgat acgggcggcg atctgcattc ccgtaacctg gaaaaaccgc tggctgccgt
     1681 gcagatgggc ctgatctatg tgaatccgga aggtccagac gggaatcccg atccgatcgt
     1741 tgccgcgaaa gacatccggg atacctttgg ccgaatggcc atgaacgatg aagaaacagt
     1801 ggcgctcata gcgggtggcc atactttcgg caagacccat ggcgccgctc cggccacgca
     1861 tgtcggcaaa gagccggaat ccgctggcat ggaaatgcag ggcctgggct ggcataacga
     1921 ttaccgttcg ggaaaaggcg ccgacaccat caccagcggc ctcgaagtga cctggtcgaa
     1981 aaccccaacg cagtggagca acagttattt cgaaaatctg tttggctttg aatgggaact
     2041 tacgaagagt ccggccggcg cgcaacagtg ggttgctaaa aatgcctcat ctgttattcc
     2101 cgatgcgcat gatcgttcaa aaaaacattt gcctaccatg ctgaccaccg acatctcttt
     2161 acgggttgat ccggtctacg aaaaaatatc ccgccacttc ctggaaaatc cggaagcctt
     2221 tgcagacgct tttgcccggg cctggtttaa gttaacgcac cgcgatatgg gaccccgcgt
     2281 ccgttatctc ggccccgatg tgccggaaga agaactgctc tggcaggatc cggttccggc
     2341 ggttgatcat ccactcgtgg atgaaaaaga tatttccgcc ttgaagtcaa aagttttgga
     2401 atccgggttg agcgtatccg aactggtagc caccgcatgg gcttccgcct ccagctttcg
     2461 cggatccgac aagcgcggcg gtgcgaatgg cgcccgcatt cgcctggcgc cccagaaata
     2521 ctggcggatg aacaatccgg cgcagctgca gaaagtattg aacgtactcg aacgcattca
     2581 aaaagaattt aacgcggcgc aggcaggtgg taaaaaagtt tcgatagcag acctgatcgt
     2641 gcttgccggc ggcgcagggg ttgaaaaagc agcgaaggat gctggacagg cgatcgccgt
     2701 tccgttcacg gccggccgca ccgatgccgt tcaggaacaa accgatgtgg aatccatagg
     2761 cttcctggaa actccgggcg atggtttccg caactaccgc aaatcaaaat tcccggtatc
     2821 caccgaagaa ttgctgatcg ataaggcgca gctgctcacg ctcacagcgc ctgaactcac
     2881 cgttctggtg ggcggcatgc gggtattgaa tacgaatttt gatggttcca acgatggcgt
     2941 tttcacgctt cgcccgggtc agctgaccaa tgatttcttt gtcaatctgc tcgacatgcg
     3001 tacgcaatgg aaagccgtat ccgccgacca ggaactgttt gaaggcagcg accgttccag
     3061 cggaaaagta aaatggaccg gcacccgggc cgacctggta tttggttcta acgccgaact
     3121 gagagctgtt gccgaagtat acgcgagtgc cgatgcgcag aagaaatttg tgcaggactt
     3181 cgtcgcagcc tggaataaag tgatgaacct ggaccgcttt gagctggcct gaggggaaag
     3241 ataaaagcgg aataagcgct ttgccggaac ggccaggcgc ttattctgtt ctcaggtttt
     3301 ttaccgggtt tgccagcgcc gcttttatcg actggtaact gacggtgagc aatgcgatca
     3361 atacggataa aaggccggct aaggcaaaca cccaccattc cacgcttacc cggtacgtat
     3421 aattctgcaa ccaggtatac atgccccacc aggccagggg tgcggcaatt aagaaggaaa
     3481 tgacaaccag ttttacaaaa tccttcgata acagcgtggt gatgccggtc agggaggcgc
     3541 ccaatatttt tcgtatacca atttctttca cccggttttc ggccatatag gtggccaggc
     3601 caaataaacc caggcaggaa ataaatacgg tgaggccggc aaacaagccg gcgagcgtac
     3661 cctgccgctt ttcattctca aactttttgg cgtaggcttc atccacaaat cgggcatcga
     3721 aaggatattc cgggttatat tttttgaaaa tagcttccgc tcttttcaga ttttgcgccg
     3781 tgccgttatt tccattaagc tttatctgca tcacgttgaa ggtcatgaac tgactctttg
     3841 caccgcagat cagtatcggc cgggtaggtt cgtacgggct tgtcaggata aagtctttga
     3901 taacgcccac aatatgccat tcaatgccca ggtctccgac gatcttacca atcgggtttt
     3961 tgaacttcat cactttcagc gccgattcat ttatgatcag tccggttgag tcggttggat
     4021 attgtttcag atcgaaatcg cgtccctgca caaattgcaa acctgccgta acgcccaacc
     4081 cctcatcctc caggtaccgg tcgaaagagg tttgatcatt cgggtctttt ccttcccagg
     4141 cctgtcccca tccatcgctc catctttcgg ttagcggaga gttcgttttg gttaccgatt
     4201 tcgcaatacc ggatgaaagc agttcatttt ttatcagtgt atagttcttc gggatatcgc
     4261 ccgtcatgaa ttgataaatg agattgtcct tgtcgtatcc ggtctcgcgg ttccgggcat
     4321 agtcgatctg ctgcttaacg atgatcgtgc agatgatcag gatgatcgca aaggtaaact
     4381 gcaggaccac gagcactttc ctcggcgtta ccagcgcatt ggccttctta aaagatccct
     4441 ttaacacttt cacgggccgg aaggaggaaa gaaagaaggc ggggtaactg ccggccagca
     4501 aacccgtgaa caggataaag ccggtaaatg ccatccaggt ataggcattc ccgaagtcta
     4561 taaacaattc tttctccgta agccggttat atgccggcag gctgaactga acaatgataa
     4621 cggcaatgat ccctgccagc agggtcagaa aaaccgattc cccgatgaac tgggcgatca
     4681 gtgaaccttt ccgcgcgccc accactttcc gtatccccac ttctttcgcc cgtttttcgc
     4741 tccgcgcggt gctgaggttc ataaaattga tacaggcgat caacaggata aaggctgcta
     4801 tgatgctaaa cagttttacg aaagtgctgc gtcccccgct gtcttccact ccatcggtaa
     4861 agctggaata caaacgccag cgatcgaggg ggtagagaaa cagttcccat ttcatgttct
     4921 ttgctccatc actgtatttc tgcttcaacg tttttatccg ggattccacc gcagcgaagc
     4981 ttgatttggg tttcagcagc acataggtcg gcgtgctgtt gtcgttccag cctaaatcct
     5041 ggcccacccc gtattttaga ttggcgatgg atatcaggaa ctcaaaatcg aagcgggtat
     5101 tatttggcag atctttcagc acgccggtaa tggtaaagtc tccctggtct ttcagcctca
     5161 ccagcctgcc catggcatct tccttaccga acaggctttt ggccgctttt tccgtcagga
     5221 ccattccatg aatattattc agggccgtag cgggattgcc ctgcagcatc gggaagctga
     5281 acatttgaag gaaacccgta tccaccatgt tcccggattt caacattttt ttatcgccaa
     5341 cggaaaacag gaaactgctc gtattcacca cccttaccgc gcgctccact tcgggcaggt
     5401 ccttttccaa tacgcgggcc agcggggccg atacggaatt ccagctgcgg atcgtaccct
     5461 gcatgggcac gcggttccac acttcgtaaa ttctctcttt gttctgatga aactggtcgt
     5521 agctgatttc attccggatc cacaacaaaa tgaggatggc cgccgccatt cctactgcca
     5581 gaccggtaat gttaattgcc gaaaaaccct tgctgcgcaa tagattccgg tacgcgattt
     5641 ttaagaaatt cctgatcatt gtaattaatg cttcgttgaa atgtgaccgt cgaaacacgc
     5701 atgatgcgca tcagccctct gttctcaaac tctccacggg gtttgctatt gctgctttta
     5761 ttgcctggaa actgattgtt agcagggcga taattattgc agccagtccg ccggcagcaa
     5821 acagccacca gctgatattg atacgatagg cataatcctg cagccattga tgcatgtaat
     5881 accaggcaat gggagcagca accgcaaagg caatcgcaat gagcaggata aattccttcg
     5941 aaaacaaata aacgatactg cccgccgtgg cgccaagcac tttacggata cccacttctt
     6001 tt
//
LOCUS       DASYCU010000190         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4970694, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000190 DASYCU010000000
VERSION     DASYCU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4970694"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..522)
                     /locus_tag="VGM24_03830"
     CDS             complement(76..522)
                     /locus_tag="VGM24_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154523.1"
                     /translation="MKKIILSSAFLCLGLLFTKAQAQPGFQVHVNLGIQPAWIPPAYA
                     GEDYYYLPDIQAYYCVPQRQFVYQEGGRWCFAAALPERYRGYDLYHGNRIAVREARPY
                     LHDNIYRERYGAYHNNYQRFPDRRFVERQHDYRSGYGNDHHRGGRY"
     gene            643..984
                     /locus_tag="VGM24_03835"
     CDS             643..984
                     /locus_tag="VGM24_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4154524.1"
                     /translation="MMRRDVFQAIADPTRREILHLVSRKPLNVNAVSENFEVSRAAIY
                     KHIRILTECGLLVLRPQGRERFCEARMEKLHEVSDWVDQYRQMWTGKLDRLEKYLASL
                     DPMTKKTRKRK"
     gene            1009..1398
                     /locus_tag="VGM24_03840"
     CDS             1009..1398
                     /locus_tag="VGM24_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4154525.1"
                     /translation="MKKTNIIFWVSTILVAGLFGMSAVSGILSADDSVKLIVNQMGYP
                     KYILPFLGVAKALAIIAILVPGFPRIKEWAYAGLAFDLAGAMYSSIALGEPVKAWIWF
                     VLFFLVLGISYTYYHRKLNASRRLVNQ"
     gene            complement(1384..2370)
                     /locus_tag="VGM24_03845"
     CDS             complement(1384..2370)
                     /locus_tag="VGM24_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4154526.1"
                     /translation="MKIISILVPRGDVALSCIEGSFTVFNKVNDFLKLAGKPPLFKVQ
                     LVGLTREPEEYHRLFQVQPDFTIDQVKKTDLIIIPAVNGDMEKVIEMNADFIPWMVKL
                     HHRGAELASLCVGSFILASTGLLKGKKCSTHWQAVDHFRRMFPEVNLVSEKIITDEQG
                     IYSSGGANSFLNLILYLVEKYAGRDMAVYASKVFEIEIDRLTQSPFIIFGGQKDHDDE
                     PILKAQEFIENNFQDRISVEQLASMLAISRRNLERRFKKATSNTALEYMQRVKIEAAK
                     MSLESSRENVNEVMYKVGYTDTKAFRNTFKRITGLSPVEYRNKYYRENLLVN"
BASE COUNT          654 a          686 c          678 g          653 t
ORIGIN      
        1 aaaataaaaa cagcccggat atccttccgg gctgatgacg attactccac gatgatctga
       61 gatgccggat catttctagt accggccacc acgatgatgg tcgttgccat aaccactccg
      121 gtaatcatgc tgccgttcca caaaccggcg atcggggaaa cgctggtagt tgttatgata
      181 cgctccatac ctttcccggt atatattgtc atgcaaataa ggtctcgctt cgcggacggc
      241 tattctattg ccgtgataga ggtcataccc gcggtatctt tcgggcaggg cagcggcaaa
      301 gcaccagcgg ccgccttcct gataaacaaa ctgccgctgc ggcacgcaat aataggcctg
      361 aatatccggc agataataat aatcctcgcc ggcgtaagcc ggcggaatcc aggccggctg
      421 gatacccagg ttgacatgaa cctgaaatcc cggctgggcc tgcgctttgg tgaacaatag
      481 gccaaggcat aaaaacgcgg aggacaggat gatctttttc atggtataac tttcttataa
      541 gaccacggcg cgaaaagccg gtttaaaccc cttctgttag ggaaaacaaa aaaaaatccc
      601 acaaatcaaa aggatcttta tattgcaacc taaaggtttc atatgatgcg cagagacgtt
      661 tttcaggcca ttgccgaccc tacccggcgg gagatcctgc acctggtttc acggaaaccc
      721 ctaaacgtaa atgccgtctc ggaaaatttc gaagtgagcc gggcggccat ttacaaacat
      781 atccggatat tgaccgaatg cgggttgctg gtgctgagac cgcagggacg cgagcgcttt
      841 tgtgaagccc gcatggaaaa gctgcatgaa gtatctgact gggtggatca gtaccggcaa
      901 atgtggacgg gtaaactgga ccgcctggaa aaatacctgg ccagtctcga cccgatgacg
      961 aaaaaaacac gtaaaagaaa atagattccc aacaaacaaa acaacaacat gaaaaagaca
     1021 aacatcatct tctgggtttc caccattctc gtggcgggtc tcttcggcat gagtgcggtg
     1081 tccggcattc tatcggcgga cgattcagtc aaactgatcg tcaaccagat gggttacccg
     1141 aaatatatcc tgcccttttt gggcgtggcc aaagcgctgg ccatcattgc gattctcgtt
     1201 cccggttttc cccgaatcaa ggaatgggct tacgctggcc tggcttttga tctggccggg
     1261 gcaatgtatt ccagcatcgc tttgggcgag ccggtcaaag cgtggatctg gttcgtcctg
     1321 tttttcctgg tgctgggcat ttcctacacc tattaccacc ggaagctgaa tgcctcccgc
     1381 cgcttagtta accagtaggt tttcgcggta atatttgttc cggtattcca ccggcgacag
     1441 gcccgtgatc cttttaaagg tgttgcgaaa ggctttcgtg tcggtatacc cgactttata
     1501 catcacttcg ttcacatttt cacgggaaga ctccaggctc atcttggcgg cttcgatctt
     1561 tacccgttgc atgtattcaa gtgccgtgtt ggacgtggct tttttaaaac gccgctccag
     1621 gttgcggcgg ctgatggcca gcatggaagc cagctgttca acgctgatcc ggtcctgaaa
     1681 attattctcg atgaattcct gggccttcag gatgggttcg tcatcgtggt ctttttgtcc
     1741 cccgaaaata atgaacggtg actgcgtcag ccggtcgatc tcgatttcaa atactttgga
     1801 agcatacacc gccatgtccc ggccagcgta tttttccacc aaatagagga tcaggttgag
     1861 aaaggaattg gcgccgccgc tcgaataaat gccctgctca tcggtgatga tcttctcaga
     1921 caccaggttg acctcgggaa acattcttcg gaaatgatcg accgcctgcc agtgggtgga
     1981 gcattttttc cccttgagca aacccgtcga cgccaggata aagctgccca cgcaaaggct
     2041 ggccaattcg gcgccccggt gatgcagctt caccatccag ggaataaaat ccgcattcat
     2101 ctcaattact ttttccatat ccccgttcac ggcggggatg atgatcaggt ctgtcttttt
     2161 tacctgatcg atggtgaaat cgggctgcac ctggaacagg cggtggtatt cctcgggctc
     2221 ccgggtgagt ccgacgagtt gcaccttgaa taacggcggc ttgcccgcca gcttcaaaaa
     2281 atcattcact ttattgaaaa ccgtgaacga gccttcgatg caactcaggg ccacatcacc
     2341 ccttggcacg aggatggaaa ttattttcat gaatcaaaaa tagagaaaat atctggcgca
     2401 ttttgcccgc agattgccgt ttttgccccc cctgcgcttc cggggccgga tgtaagtttg
     2461 tgtcgtaatg aaacatttga tgcagggcgc gaaaagtaac agagacatga atgaattttg
     2521 tattacgcgg caatttgatg ccggaaaaga cctgctcttc agagcctgga cagacccgga
     2581 tctgctgaag cactggtggg gaccgaagga tttccattgg gtgggcggga aactgaatct
     2641 ctatcccgga ggcatgttcc actactgtat g
//
LOCUS       DASYCU010000191         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2943238, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000191 DASYCU010000000
VERSION     DASYCU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943238"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VGM24_03850"
     CDS             <1..400
                     /locus_tag="VGM24_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY4154527.1"
                     /translation="NYNLYKKFTVSGNINFNEMKANKTDDIFVTGFNTPRWSTNLSFG
                     NREVVENVGFNVTWRWQDAFLWESPLVTGNVSAYSTLDAQVTFHLPKLKSAVKVGASD
                     IFNHRYIQYAGGPTIGGLYYVAITVDGLLK"
     gene            613..>1520
                     /locus_tag="VGM24_03855"
     CDS             613..>1520
                     /locus_tag="VGM24_03855"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY4154528.1"
                     /translation="MSNLSTIAIPGFPEKLAALQSQVGNTPLFEIRNLFRKKKVSLYA
                     KQEWMQLSGSVKARAAYAIFRDAIHKGQLGHGKILLDATSGNTGIAYAHIGKSLGIPV
                     TLCLPENASGERKEILKSLGVELILTSRFEGTDGAQEIARTLAEENPGRYFYADQYKN
                     ENNWKAHYYGTAVEIIETLPGITHFVAGLGTTGTFVGTGRRLKKYRPAIQLISLQPDN
                     PLHGLEGWKHLETAVVPEIYDPAVADANLEVSTEEAYQLILEAKEKEGLELSPSSAAN
                     IAGAIRVASGLEEGVVVTVLPDNGDK"
BASE COUNT          416 a          363 c          369 g          372 t
ORIGIN      
        1 taactataat ctgtataaga aatttacagt ttccggaaat atcaatttca acgagatgaa
       61 ggccaataaa acggatgata tttttgtcac cggcttcaat accccccgct ggtcaaccaa
      121 tctttccttc ggaaacaggg aagtcgtaga aaatgtcgga tttaatgtta cctggagatg
      181 gcaggatgcc ttcctctggg aaagtcccct ggtaaccgga aatgtgtccg cttacagcac
      241 cctcgatgcc caggtcactt tccatcttcc caagctgaaa tctgccgtta aagtgggtgc
      301 atctgatatt tttaaccacc ggtatatcca gtatgccggc gggccgacca ttggagggct
      361 ctattatgtt gccattaccg tggacggttt attaaaataa agaaagaatg tccgtgttcc
      421 accatttaat gaacaaaacg actatctttg tgttagtaag taatcatgca aatgagttca
      481 gatacgcttc acacctgttg ttgttgttgt gcagaacgcc ggatttatcg ggtggagggt
      541 attcgtatat agattaaaaa acgacaaata atactaaagc tccacccatg tggggctttt
      601 ttattttacg ccatgtccaa cttatcaacc atcgccattc cgggatttcc ggaaaagctg
      661 gctgccttgc agtcgcaggt cggaaacacg ccgcttttcg aaatccggaa tcttttccgg
      721 aagaaaaagg tatccctcta tgccaagcag gagtggatgc aattgtcagg cagcgttaaa
      781 gcacgggctg cgtacgcaat tttccgggat gccattcaca agggacagct tggtcacggc
      841 aaaattctgc tggacgccac cagtggcaat acgggcattg cctatgccca tatcggcaaa
      901 tccctcggaa taccggtgac cctttgcctt cctgaaaatg cttcgggaga gagaaaagaa
      961 atcctgaagt ccctgggggt ggaactgatt ctcacatcgc gttttgaggg gacggacgga
     1021 gcccaggaaa ttgcccgcac ccttgctgaa gaaaatcccg gccgctattt ttacgccgat
     1081 caatacaaaa atgaaaataa ctggaaagcc cattattatg gtaccgcggt ggaaatcatc
     1141 gaaacgcttc cggggatcac ccatttcgta gcgggcctcg gcaccacggg cacttttgtc
     1201 ggaacgggca gaagattaaa aaaataccgt ccggcgatac agttgatctc cctgcaaccg
     1261 gataatccat tgcatggcct cgaaggctgg aaacacctcg aaactgcggt ggtacctgag
     1321 atttatgatc ctgctgttgc agacgcgaat ctcgaagtct ccacagaaga agcatatcaa
     1381 ttgatcctgg aagcaaagga aaaagaaggt ctggaactaa gcccctcatc agctgcgaat
     1441 atcgccggcg ccatccgtgt ggcttcaggt ctggaggaag gcgttgtggt tacggttctg
     1501 ccggataacg gcgacaaata
//
LOCUS       DASYCU010000192         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3379238, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000192 DASYCU010000000
VERSION     DASYCU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379238"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VGM24_03860"
     CDS             <1..801
                     /locus_tag="VGM24_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEY4154529.1"
                     /translation="ADVCHLNLHKTFSIPHGGGGPGMGPICVNEKLKDHLPGHRINSN
                     HGGSGPVSAAPYGSASILLISYAYIKMLGSAGITDATRYAILHANYMKSRLETHYPIL
                     YAGINNTCAHEFIVDLRPFKAQAGIEAEDVAKRLMDYGFHAPTLSFPVPGTMMIEPTE
                     SEDKSELDRFCDAMIAIHGEMQEIADGKMDKSDNPLKHAPHTQGVICTDEWNHAYGRE
                     RAAFPLAFTRANKFWPSAGRINNTYGDRNLICTCEPVSHYETAPIGQE"
     gene            complement(813..1847)
                     /locus_tag="VGM24_03865"
     CDS             complement(813..1847)
                     /locus_tag="VGM24_03865"
                     /inference="COORDINATES: protein motif:HMM:NF025749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 domain-containing protein"
                     /protein_id="HEY4154530.1"
                     /translation="MRKKWTFCVLLFMISFPAARAQTPDHDTAYYRTYRDQVTTRAYL
                     SRKYTVLRFDPPGESPKFVYNPNTSLNLGIGATYHAFTLNIGIGISKFNPGEIKGKTR
                     YLDLQGHFYARKWNIDVLGEFYRGYYLTPKGLAAPPGESYYTRRDLGLGLLGLAFYRS
                     LNDRRFSYQAGLLQNEWQKKSAGSVLVGGEIYYGSVHADSTLVPAELDSGYGKQNINR
                     VHFFEIGPGIGYAYTLVIGQHFYALASVTVNLALRFSTEYSDPRGRYRSKTDFTPNDI
                     IHAGLGYNTAKWGLTALWVSDRLNVRGGSSGYQYGVATGNYRLIFARRFKVNRKVKKV
                     LAPIPDIIGK"
     gene            1971..>2623
                     /locus_tag="VGM24_03870"
     CDS             1971..>2623
                     /locus_tag="VGM24_03870"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY4154531.1"
                     /translation="MMAAGLPGVFKEMFFFSTETFTTVGYGRVNPVGDAANSVAAMES
                     MLGFLSFAVATGLIYGRFSKPKAFLLFSKNALVSPYRGGKALMFRFATYKDNHTLTNL
                     EIKVNAAMLLEENGARVYKFYDLDLERSRVESLPMNWTIVHPLDEKSPLYEYSADDMK
                     MTDVELYVSVRGFDDVYSNIVQQRTSYRFDEILFNRKFVQMYRESENGRTTIVELHR"
BASE COUNT          642 a          673 c          672 g          636 t
ORIGIN      
        1 gcggatgtct gtcatcttaa cctgcataaa accttctcca tcccccatgg gggtggcggc
       61 ccgggcatgg ggccgatttg cgtgaatgaa aaattaaaag atcacctgcc gggacaccgg
      121 atcaattcca atcacggagg atccggaccg gtgagtgcgg caccctatgg ttcagccagt
      181 attttactga tcagttatgc ctacatcaag atgcttggtt cggccggaat aaccgatgcc
      241 acccgctatg ccattctcca tgcaaactat atgaagtccc ggctggaaac ccactatccg
      301 atcctgtatg cgggtatcaa taacacctgc gcgcatgaat tcattgtcga tctccggccc
      361 ttcaaagcgc aggccggtat cgaggccgag gatgtagcca agcgcctgat ggattatgga
      421 tttcacgccc ccaccctgag tttcccggtg ccgggaacca tgatgatcga gcccactgaa
      481 agtgaggata aatccgaact ggaccgcttt tgtgatgcca tgatcgccat tcacggcgag
      541 atgcaggaga tcgcggatgg aaaaatggat aagtcggaca atcccttaaa acacgcgccg
      601 cacacgcagg gagtcatttg caccgatgag tggaatcacg cgtatggccg cgagagagct
      661 gcgttcccct tagcctttac cagagcaaac aaattctggc cgtcggccgg acggataaat
      721 aatacctatg gcgaccggaa cctgatatgc acctgtgagc cggtaagtca ttatgagacc
      781 gctccgatcg gccaggagta aaaatttaat atttatttcc cgatgatgtc cgggatcggc
      841 gccagcactt tttttacctt ccggttaact ttaaaccgtc tggcgaaaat aagccggtaa
      901 ttgccggtgg ccaccccgta ctggtatccg gaactacccc ctctgacatt taagcggtcg
      961 gagacccaaa gcgcagtcaa tccccatttt gcagtattgt agcccagccc ggcatgaata
     1021 atatcgttgg gagtaaaatc ggttttgctc ctgtatcttc cccgcggatc cgaatattcg
     1081 gtcgagaacc gcaaggccag gttaacggtt acggaagcca gcgcgtagaa atgctgtccg
     1141 ataactaacg tataggcata accgattcca ggtccgatct caaaaaaatg cacgcggttg
     1201 atattttgct taccgtatcc ggaatccagt tcagcgggca ccagtgtact gtccgcatga
     1261 acagacccat aataaatctc ccctcccacc agaacggaac cggcggactt tttctgccat
     1321 tcattctgta atagtccggc ctggtaagag aaacgccggt cgttcaggct gcggtaaaaa
     1381 gcaagcccga gaagacctag ccccagatcc cttcgcgtat aataggattc tccgggcggg
     1441 gcagccaggc cctttggagt gaggtaatat ccccggtaaa attcacccag cacgtcgata
     1501 ttccatttgc gggcatagaa atgaccctgc aggtccaggt acctggtttt ccccttgatc
     1561 tcccccggat taaacttgct gatcccaatt ccgatattca gggtgaacgc gtgataggtg
     1621 gcaccgattc ccagattcag gctggtgttt gggttatata cgaacttggg gctttcgccc
     1681 gggggatcaa aacgcagtac cgtatatttt ctggagagat aggcccgggt cgttacctgg
     1741 tcccggtagg tacggtaata agccgtatca tgatccgggg tctgcgcccg ggcagcagga
     1801 aaactgatca taaacagcaa aacgcaaaac gtccatttct tcctcattcc ttcctcattt
     1861 aaacagcttc cgcttacctg gttccactcc ttcactctga tttttacctg agcgtaaatt
     1921 ttctgtttgc ctggatttac ttttttatag gcagagcgca gttccaggga attatggctg
     1981 ccggtttgcc gggcgttttt aaggagatgt tctttttcag taccgaaaca ttcaccacgg
     2041 tgggatacgg aagggtgaac ccggttggag atgccgccaa ttcggtggct gccatggaat
     2101 cgatgctggg tttcctctcc tttgccgtgg cgacgggact tatttacgga cgtttttcaa
     2161 aacccaaggc ctttctcctg ttcagcaaaa atgcactggt gagcccctac cggggcggga
     2221 aagcgctgat gttccggttt gctacgtata aggataacca cacactgacc aacctggaaa
     2281 tcaaggtgaa tgcggcgatg ctgctggagg aaaatggtgc ccgggtttat aaattctacg
     2341 acctggacct ggaaagaagc cgggtggaaa gcctgcccat gaactggacg atcgttcacc
     2401 cgttggatga aaaaagtccg ctgtatgagt attctgcgga cgatatgaaa atgacggatg
     2461 tggagttata tgtttccgtg cggggctttg atgatgttta ttccaatatc gttcagcaac
     2521 gaacttccta tcggtttgat gaaattctgt tcaaccggaa atttgtacag atgtaccggg
     2581 aaagcgagaa cggcagaacg accatcgttg aactgcaccg gtt
//
LOCUS       DASYCU010000193         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3959475, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000193 DASYCU010000000
VERSION     DASYCU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3959475"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VGM24_03875"
     CDS             complement(<1..655)
                     /locus_tag="VGM24_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HEY4154532.1"
                     /translation="MISQQTIQQILSRIDITEIIGGFVKLKKRGANYLGLCPFHNEKT
                     PSFTVSPAKEIYKCFGCGRSGNTISFLMEHEKYSYVEALKWLAAKYNVEIEETESSPE
                     VKIQQQTADSLYILNGFAQQFFSGVLEQDEEGRNIGFSYLMERGFRDDIIRKFQLGYD
                     PASADAFASHALSSQYSPEILQKSGLVVWRDGKAFDNYRGRIIFPIHSQTGKITGFGA
                     "
     gene            720..2162
                     /locus_tag="VGM24_03880"
     CDS             720..2162
                     /locus_tag="VGM24_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4154533.1"
                     /translation="MESLMLKLENVEVSLAGKKILNGISWQLRKGEQWALLGASGSGK
                     TVLAQTIAGKHFFTGRMTGHFKELDSFQRKVCIVEQQHRFKNLRNQSDFYYQQRYNAQ
                     DASGTIIVREDLEDYPDTATTGFRKEELIHLFHLEELLAEPLIQLSNGENKRIQLVKA
                     LLTEHTLLILDQPFTGLDVEGRGLLSRILNRLAEAGEQMILITSARDIPECFNQVAEM
                     ENGIIRRRDPQDFQLSGSPAVVFSRENFPSRIPFHYPDYAFAVRMQDVNVRYEEKSIL
                     RSINWQVKKGECWSLSGPNGAGKSTLLSLITADNPQAYANEIYLFDRRRGTGESIWEI
                     KQKTGFLSPELHLYFDRSSTAYGSLASGLWDTIGLFRTLSEEQHELVLEWLDFLGLGQ
                     YRNSLLSNLPAGLQRMLLLGRAMIKTPPLLVLDEPCQGLDQDHIDLVRGLIDRYCEYF
                     GATLIYVSHYPEELPGSVNQFLRLKKGQIV"
     gene            2436..2639
                     /locus_tag="VGM24_03885"
     CDS             2436..2639
                     /locus_tag="VGM24_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154534.1"
                     /translation="MSLIKVHLETEQCMYVSKYQDEPAVEWKKKGDPIFDILELVGDD
                     KIKDWESIYNDNQGSYNYEEDED"
     gene            complement(2636..3301)
                     /locus_tag="VGM24_03890"
     CDS             complement(2636..3301)
                     /locus_tag="VGM24_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154535.1"
                     /translation="MKTGLLPVLNLLISILAIGLCFIPMVLFWLKRLTPDKSYFFIAL
                     FWLINGITYTPEIFRWEWYRTVSNDITLGYNLIDTPLILLIFYFAFRKKIFQQLLIGF
                     IFFEIVMIVWKGYNFGSNTPIIGLGSLISLILNIWGISVFFQKMRHTAFENVMAFVNA
                     GFIFYYGLFTVIYIFNYLNFSKVTLPYVTFINYLSITIATALISYGYQRHAVSPLKEA
                     NSR"
     gene            3514..>4119
                     /locus_tag="VGM24_03895"
     CDS             3514..>4119
                     /locus_tag="VGM24_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4154536.1"
                     /translation="MAENKQNLFEYTEASIRSLDWKEHIRLRPGMYIGKLGDGSSADD
                     GIYVLVKEVLDNCIDEYTMGYGKQIDVVIEEKQVTIRDYGRGIPLGKVVDVVSKINTG
                     AKYDSKAFQKAVGLNGVGTKAVNALSQAFTVTAFREGKEKKAEFSKGNLVKEYKEART
                     SEANGTLVQFVPDESIFKNFHFINEYLDNQFWNYCYLNAGLL"
BASE COUNT         1159 a          882 c         1031 g         1047 t
ORIGIN      
        1 ttgctccgaa tccggtaatt tttccggtct gactgtgaat gggaaaaatg atccggcccc
       61 ggtaattatc aaatgctttt ccatctcgcc aaacgaccag tccgcttttt tgaaggatct
      121 ccgggctgta ctgcgaagag agggcatggc tggcgaaagc atctgctgaa gcgggatcat
      181 atcccagctg gaatttccgg atgatatcat cccggaaccc gcgctccatc agataggaaa
      241 agccgatatt ccggccttct tcatcctgtt ccaggacgcc ggaaaaaaac tgttgggcaa
      301 aaccgttcag gatatacagg ctgtccgccg tctgttgctg gatctttact tcggggctgc
      361 tctcagtttc ctcaatctcc acattatatt ttgccgccag ccatttgagc gcttccacat
      421 aagaatactt ctcatgctcc atcagaaaac tgatggtatt gccgctgcgc ccgcatccga
      481 aacacttata gatctccttt gcgggcgaaa cggtaaagga gggagtcttt tcattatgaa
      541 acggacagag gccgaggtag ttggctcctc ttttcttaag tttcacaaag cccccgataa
      601 tctccgtaat atcgatgcgg ctgagaatct gctgtatggt ttgctgggaa atcaataacc
      661 ggttttgagc gtcgaattta atttaaaaaa ccatctttgc agccgttaaa taggacggga
      721 tggaatcctt gatgctgaaa cttgaaaatg tagaagttag cctggccggc aagaaaatac
      781 tgaacgggat ttcctggcag ctcagaaagg gagaacaatg ggccctgctc ggtgcatccg
      841 ggtcgggcaa aacggttttg gcccaaacga tagcggggaa gcattttttc acgggaagaa
      901 tgaccggcca ttttaaagag ctggattctt ttcagaggaa agtgtgtata gtggaacagc
      961 agcaccggtt taagaatctc cgtaaccagt ccgactttta ttatcagcag cgttacaatg
     1021 cgcaggatgc ctccggaacg attatcgtgc gggaagacct ggaagattat cccgatacag
     1081 cgacaaccgg attccggaag gaggaactga tccacttgtt tcatcttgag gaattgctgg
     1141 ccgaacccct gatccaattg tcgaatggcg aaaacaaaag aatccagctg gtcaaggccc
     1201 tgcttaccga gcataccctg ctgatcctgg atcagccttt taccggattg gacgtggaag
     1261 gccgggggct tttatccagg atcttaaacc gccttgcgga agcgggagaa cagatgatcc
     1321 tgatcacttc agccagggac attccggaat gtttcaacca ggttgcggaa atggaaaatg
     1381 ggatcatccg caggcgtgac ccgcaggatt ttcaactctc cggttctccg gctgtggttt
     1441 tttcccggga aaattttccc tcccgaattc cgtttcatta cccggactat gcgtttgccg
     1501 tgcgaatgca ggacgtcaat gttcgctatg aagaaaagtc cattcttcgc agtataaact
     1561 ggcaggtaaa aaagggggaa tgctggagct tatccggacc gaacggagcg ggcaaatcca
     1621 ccttgctcag cctgatcacc gccgacaatc cgcaggctta cgccaatgaa atttatttat
     1681 ttgaccgaag gcgcggcaca ggggaaagta tctgggagat caaacaaaaa accggttttc
     1741 tctcgcctga actccattta tattttgacc gctcatcgac cgcatacggt tcccttgctt
     1801 ccggcttatg ggatacgatc ggtttatttc gtactctttc agaggaacaa cacgaactgg
     1861 ttttggaatg gcttgatttt cttgggctcg gtcaataccg gaacagcttg ttgagcaacc
     1921 tgcctgcagg cctgcagcgg atgcttctgc tgggccgcgc tatgatcaag acgccgcccc
     1981 tgctcgttct ggatgaaccc tgccagggcc tggatcagga tcatattgac ctggtcaggg
     2041 ggctgatcga ccggtattgc gaatatttcg gggctaccct gatctatgta agtcattacc
     2101 cggaagagct gccgggctcg gtcaatcaat tcctgcggct gaaaaagggt caaatagtct
     2161 aagtcattga taatcaggcc taattaaagg cagaataaat acctttccac ccaagtgtaa
     2221 aaacctgaaa cgggatatgg aaaaacagta aatttgcata gagctgcagt tttaacctac
     2281 ttacccataa ttctcctgca gattaagttt taatacttcc cattaattca gaagatccag
     2341 accgaaatca ttattaaaac tagtgtagta tgtattctta tgctctgtta gaaaaaaact
     2401 gttactatct cattcaggaa actgaagagt cacccgtgag tctgattaaa gtgcatctgg
     2461 aaacagagca atgcatgtat gtttccaaat accaggatga gccggctgtt gaatggaaaa
     2521 aaaagggaga cccgattttt gatatcctgg aacttgtcgg tgacgacaag attaaagact
     2581 gggaatcgat ttataacgat aaccagggaa gttataatta tgaggaagat gaggactagc
     2641 gtgaattcgc ttctttcagg ggagagacgg catgccgctg gtatccgtag gaaatgaggg
     2701 ctgtggcgat cgtgatgctc aggtaattga taaacgttac gtacggcagg gtgaccttgc
     2761 tgaaatttaa atagttgaat atatagataa ccgtaaaaag accgtagtaa aagataaagc
     2821 ctgcgtttac aaaagccatt acgttttcga atgcggtatg acgcattttt tgaaagaaga
     2881 cgctgatccc ccagatattc aggatcaggc tgattaaact tcccagtccg ataatcggtg
     2941 tattggaacc gaaattatat cccttccaga cgatcatgac gatttcaaaa aagatgaatc
     3001 cgatcaggag ctgctgaaag atctttttcc ggaaggcaaa ataaaagatc agcaggatca
     3061 ggggggtatc aatgaggtta tatcccaggg taatatcatt gctcacggtc cggtaccatt
     3121 cccagcggaa aatctcgggg gtataggtga tcccgttaat cagccagaaa agggcaataa
     3181 agaaatagga tttgtccggc gtcagccttt taagccagaa taaaaccatg gggatgaagc
     3241 aaagcccgat agcaagtatg gagatgagca ggttgagaac cggtaacagg cccgttttca
     3301 aaggtaagag tttagactat aatagcgttg aaagacctcc ttttagtata ggtaaaacta
     3361 cgtggtttta ttctcaaatt acgcatggat tttacctgca gaatgtccgt aaaatccggt
     3421 aaatcaattt aataagattg gcaatatgca cattcgtgcg taatttatga agggattgaa
     3481 caaattcaat tacctatatt tgcgcccatc attatggcag aaaataaaca gaatttattt
     3541 gagtataccg aagcctctat ccggtcgctg gactggaaag agcatatccg tcttcgtccg
     3601 ggaatgtata ttggaaagct gggagacggc agtagtgccg acgatggtat ctatgtgctg
     3661 gtaaaggagg tgctggataa ttgcattgat gaatacacga tggggtatgg gaagcagatt
     3721 gatgtggtca tcgaggaaaa acaggtgacc atccgggatt acggtcgcgg tattcccctg
     3781 ggaaaggtgg tggacgtggt gagcaagatc aataccgggg ccaagtacga cagcaaagct
     3841 ttccagaagg ctgttggtct caatggggtg ggaacgaaag ccgttaatgc cctgagccag
     3901 gcatttactg ttactgcttt ccgcgaaggg aaggaaaaga aagcagagtt ttcgaaaggt
     3961 aacctggtaa aggaatataa ggaagccagg acgtctgagg cgaatggcac cctggtccaa
     4021 ttcgttcccg atgaatccat tttcaagaat tttcatttta taaatgaata cctcgacaac
     4081 cagttctgga attattgtta tctgaatgcg ggactgtta
//
LOCUS       DASYCU010000194         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5120103, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000194 DASYCU010000000
VERSION     DASYCU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5120103"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VGM24_03900"
     CDS             <1..1418
                     /locus_tag="VGM24_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY4154537.1"
                     /translation="RQLEILKGMGCNALRTSHNPPAPELLDLCDELGILVMDEAFDMW
                     KRPKNPFDYHLVWDEWHKQDLEDQVRRDRNHPSVFIWSIGNEIPEQRSKDSSGIAIAR
                     ELGAIVKSLDTTRPVTSALNSPYPNNAIIKSGALDLIGYNYDFKDYPDFHERFPGKKF
                     IATETVSAFETRGFYEMPSDSIKRYAHRKNIVLPDGTVTNTISAYDNASAAWGSTHEE
                     TLKMINKYKFLSGQFIWTGFDYLGEPTPYGWPSRSSYFGIIDLAGFPKDIYYLYQSVW
                     TNKPVLHIFPHWNWKEGQTVDVWAFYNQADEVELYLNGKSMGIRKKTGDDLHVMWRLK
                     YEPGTLKAVSRKNGKQVLEEEIQTAGPAAKIELLADKKTLRADGTDLDFITVKVLDKD
                     GNLVPDADQLIRFNVSDNASIAGVDNGNEVDLDSFKAKERKAFNGMCLVVVQSKKMKG
                     EIKVKATGEGLSPATINLMAE"
BASE COUNT          433 a          338 c          384 g          345 t
ORIGIN      
        1 gccgtcagct ggagatctta aaagggatgg gatgtaatgc ccttcgtact tcccacaatc
       61 caccggcgcc ggaactgctg gacctttgtg acgaattggg aatcctggtc atggatgaag
      121 catttgacat gtggaaaagg cccaaaaacc catttgatta tcacctggtt tgggacgaat
      181 ggcataagca ggacctggaa gaccaggtga ggagggaccg gaaccacccg tctgttttta
      241 tctggagtat cggaaatgaa attccggaac agcgaagcaa ggacagctcc ggcattgcca
      301 tcgccaggga actgggggcc attgtaaaat cactggatac cacccggccg gttacttcgg
      361 cactcaacag cccttatccg aataacgcga ttattaagtc gggcgccctc gatctgatcg
      421 ggtataatta tgatttcaaa gactatccgg attttcatga acggttcccg ggtaaaaaat
      481 ttattgcaac ggaaacggtt tccgcatttg aaaccagggg tttttatgaa atgccatccg
      541 acagcatcaa acggtacgct catagaaaga atattgttct tccggatggc accgttacca
      601 acaccatttc tgcctacgat aacgcttccg ctgcctgggg gtccacgcat gaagaaaccc
      661 tgaaaatgat taataaatat aaatttctct ccggccagtt tatctggaca ggcttcgatt
      721 acctcggtga acccacaccc tatggatggc cttcccgcag ctcttacttc gggatcattg
      781 acctggccgg ttttccaaaa gatatttact atctgtacca gagtgtgtgg acaaataaac
      841 ccgtcctgca tattttcccc cactggaact ggaaggaggg acagaccgtg gatgtatggg
      901 cattctataa tcaggcggac gaggtggaac tttatctgaa tggaaaatca atgggcatcc
      961 ggaagaaaac cggcgatgat ctccatgtga tgtggagatt gaaatatgag ccgggaaccc
     1021 tgaaagccgt ttccaggaaa aacggaaagc aggttttgga ggaagaaatc cagacggccg
     1081 gaccagcagc aaagattgaa ctactggcag ataaaaaaac gctccgggca gacggaacgg
     1141 atcttgattt tatcaccgta aaagtgctgg acaaagacgg aaaccttgtt ccggatgcgg
     1201 atcagctgat ccgcttcaat gtcagtgaca atgcttccat tgcgggcgtg gataacggga
     1261 acgaggtgga tctcgattcc tttaaagcaa aagaacgcaa agcctttaac gggatgtgcc
     1321 tggtggtggt ccaatcgaag aagatgaagg gcgaaattaa agtaaaggcg acgggcgagg
     1381 gtctgagtcc tgcaaccata aatctgatgg ctgaatgatc agcatttaaa aatttatcta
     1441 atgacaaccc gccggaaatt tataaaccag agtacaaaag ggcttgtttc tgcagcagtc
//
LOCUS       DASYCU010000195         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2076842, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000195 DASYCU010000000
VERSION     DASYCU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076842"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VGM24_03905"
     CDS             complement(<1..667)
                     /locus_tag="VGM24_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY4154538.1"
                     /translation="MENNKRIYPRRDFLGNMVKAGTAGLMVVPAIQSLQLEKSLTVSE
                     IIDLILQEVPGGRLTDTVDTIKAGSGDQSVTGILTTMFATVKVIEEAVRLNANFIIAH
                     EPTFYNHTDDINWVRDNQVVKQKQELLQKHQIAVWRFHDQWHRMNPDGILHGVLLKTG
                     WARYSPKDEMHFEIPRQSLGGIVDHLKTTLGIPHLRVIGDLSASCSRISLLPGAWGGQ
                     RQVE"
     gene            complement(667..1176)
                     /gene="purE"
                     /locus_tag="VGM24_03910"
     CDS             complement(667..1176)
                     /gene="purE"
                     /locus_tag="VGM24_03910"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY4154539.1"
                     /translation="MIQVAIIMGSDSDLPVMQPAADALKAFGIDFELTIVSAHRTPLR
                     LVEYAGSARERGIRVIIAGAGGAAHLPGMVASLTPLPVIGVPIKSSNSMDGWDSVLSI
                     LQMPGGVPVAAVALNGAKNAGILAATILGVSDETISAKVQAYKKSLEDEVLQKIDKLK
                     KDGWQNTFD"
     gene            complement(1173..2312)
                     /locus_tag="VGM24_03915"
     CDS             complement(1173..2312)
                     /locus_tag="VGM24_03915"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY4154540.1"
                     /translation="MLKIGILGGGQLGRMLLQAAANYPVITHVLEKDPECPAAHLCHS
                     FVQGDIRNFDDVYQFGRTLDAITIEIESVNVEALEKLESEGIRIYPAPSALRIIRDKI
                     LQKQFYKENDIPTSPFHITQNRGELQKHDGFLPAVHKQGSGGYDGRGVQVIADTRDFG
                     KGFDAPSVLERKVNIHKEISQIIAVNRQGQTAIYPAVEMIFDPVLNQLDYQLSPAEIP
                     QQTLWRIEAIAQKVVKDLKTAGIFAVELFLDKNGDVLVNETAPRVHNSGHHTIEANYS
                     SQFDMLWRVILDYPLGNPRHIIPAVMLNLVGSEGCSGEARYEGIDEVLKMENAFLHLY
                     GKKETRPGRKMGHVTILSTDKSDLVYKAKSIKKQFHIIAKQPFSL"
     gene            2444..3640
                     /locus_tag="VGM24_03920"
     CDS             2444..3640
                     /locus_tag="VGM24_03920"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY4154541.1"
                     /translation="MKKSSFFSQIGLLLASFAFLSPVFSQSSAYSVHFLDYQKSLPKV
                     SEMINRKEDTLMKQFRTKQLVWPARYVYIRSFKYDSEMEIWVKNKASDKFRLFKTYKI
                     CALAGSLGPKRMAGDYQVPEGFYYINEFNPKSLYHLSLGLNYPNASDKLLSDLSQPGG
                     DIYIHGSCVTTGCIPITDGQIEELYVLAAHAKDLGQDFIPVHIFPVNFNNPRSVAYLN
                     KFLQQFNEYVPFEKNMRNAFFYFEKNHEIPPVIVNDKGDYVTEEVQPPPTVERKPEVV
                     PVNVKKTDHPEQPIPDEELAKSVDKLPSYPGGTEAFQVFIDKLSADMVKELDPGQSKA
                     FVLMEYIIDENGKTIYANPISGGNDRMNEKIVKAFAGMGLWTPATRQGKNVPIKMKQT
                     IMVGVP"
BASE COUNT          918 a          950 c          890 g          990 t
ORIGIN      
        1 cttccacctg ccgttgtccg ccccaggcgc cgggcaacag ggagatcctg ctgcaggagg
       61 cggataaatc gccgatcacc cgcaaatggg gaatgcccaa tgttgttttc agatgatcca
      121 cgatgccgcc cagggattgc cggggaattt caaaatgcat ttcatctttc ggagagtagc
      181 gggcccaacc ggtcttcagc agcacgccat gaagaatgcc atccggattc atccggtgcc
      241 actgatcatg aaaacgccag acggcgatct ggtgcttttg aagcaattcc tgtttttgtt
      301 taaccacctg gttatccctg acccagttta tatcatctgt gtgattatag aacgtcggtt
      361 catgggcaat gatgaaattg gcattcagcc gcacggcttc ttcaatcact ttcaccgtgg
      421 cgaacatggt ggttaggatt ccggttacgg actggtcgcc gctgccggcc ttgatcgtat
      481 ccacggtatc cgtcaatctt ccgccaggca cctcctgcag gatcaaatcg atgatctccg
      541 ataccgttaa ggatttttca agctgcaggc tttgtattgc cggaaccacc atcagtccgg
      601 ccgttcccgc ttttaccata ttcccgagga aatccctgcg ggggtaaatt cttttattat
      661 tttccattag tcaaatgtgt tttgccagcc atcctttttt aatttatcta tcttctgcag
      721 cacttcatct tccagcgatt tcttgtaggc ctgaactttt gccgagatgg tttcatcaga
      781 aaccccgaga atggtggctg ccaggatacc ggcgtttttc gctccgttca gcgccaccgc
      841 ggctacgggc acccctcccg gcatctggag aatggataac acagaatccc agccatccat
      901 ggagttggaa gatttgatcg ggacgccaat caccggaaga ggggtgaggg aagcgaccat
      961 tccgggtaaa tgcgcggctc cgcccgctcc ggcgatgatt acccggattc ctctttccct
     1021 ggctgaaccg gcatattcca ccaggcggag cggggtccgg tgggcagaga cgatagtcag
     1081 ttcaaaatca atgccgaagg ccttaagtgc atccgctgcg ggctgcatta cgggcaggtc
     1141 actgtcgctt cccatgataa tcgccacctg gatcataaac taaacggttg ttttgcaatg
     1201 atgtgaaatt gctttttaat ggacttggcc ttataaacca gatcactttt gtctgtgctc
     1261 aaaatcgtaa catggcccat tttccttccg ggccgggttt cttttttacc atacaaatgc
     1321 aggaatgcat tttccatctt caatacttcg tcaattcctt cgtaccgtgc ttcaccggaa
     1381 cagccttcac tgccaaccag gttcagcatg acggccggta tgatatgcct gggatttccc
     1441 aggggataat ccaaaattac ccgccacagc atatcgaact gggaagaata gttggcttca
     1501 atcgtatgat gtccgctgtt gtgcacccgc ggtgcggttt cgttcaccag gacatctccg
     1561 tttttatcta gaaataattc cactgcgaaa atgcctgctg ttttcaggtc tttcaccact
     1621 ttctgcgcga tcgcctcgat ccgccacaag gtttgctggg gaatttccgc cggactgagc
     1681 tggtaatcga gctggttaag gaccggatcg aagatcattt ccaccgccgg ataaatggcg
     1741 gtctgcccct ggcggttaac ggcaatgatc tgcgaaattt ctttatggat gttcactttt
     1801 ctttccagca cggaaggggc atcaaatccc ttcccaaaat cccgggtatc cgctatgacc
     1861 tgcacgccgc gtccgtcata accgcccgaa ccctgcttgt gaaccgccgg taaaaaaccg
     1921 tcgtgtttct ggagctctcc gcggttttgc gtaatatgaa agggggaggt gggaatatca
     1981 ttttctttat agaattgctt ttgcaggatc ttgtcccgga tgatgcgaag ggcagaagga
     2041 gcaggatata tccggatccc ctccgattcc agtttttcca gggcctccac attaaccgat
     2101 tcaatttcaa tcgttatagc atcgagcgtc cgcccaaact gataaacatc atcaaaattc
     2161 cggatatctc cctgcacgaa cgaatgacag agatgggcgg cggggcattc cgggtccttt
     2221 tccagcacat gggtaattac gggataattg gctgcagcct gcaaaagcat cctacccagc
     2281 tggccgccgc ctaaaattcc gattttgagc atattcccaa ttatggcgtg aagataaaac
     2341 aatctggttt tcgcccgttt attaagggcg gttaacataa taatatggcc gtatttttgt
     2401 tttatatggt aactttatcc gtatcaatgc ataatttttt tgcatgaaga aaagcagctt
     2461 tttttctcag atcggccttt tactggcatc tttcgcattt ctatcccctg ttttttccca
     2521 gagttccgct tattcggtgc attttctgga ttaccagaaa tcacttccca aagtgagtga
     2581 aatgatcaac cggaaggaag acaccctgat gaagcagttt cgcaccaaac agctggtctg
     2641 gccggcccgc tacgtgtata tccgttcttt caaatacgac agtgagatgg aaatctgggt
     2701 aaagaacaaa gcttcggata aattcaggtt attcaaaacg tataagatct gtgcgctcgc
     2761 cggttccctg ggtccaaaaa gaatggccgg tgattaccag gtgccggaag gtttttacta
     2821 tatcaatgaa tttaacccga aaagccttta tcatttatcg cttggattaa attatcccaa
     2881 cgcttccgat aaattgctga gcgacctgtc ccaacccggc ggtgatatct atattcacgg
     2941 aagttgtgtg accacgggct gtattcccat caccgacggg caaattgaag aactgtatgt
     3001 gctggccgca catgccaaag acctgggcca ggattttatt cccgttcata ttttccctgt
     3061 caatttcaac aacccccgga gcgtggccta cctgaataaa ttcctgcagc agttcaacga
     3121 atacgtgcct tttgaaaaga atatgagaaa tgcgtttttc tattttgaaa aaaatcatga
     3181 aatcccgccc gtcatcgtga acgataaagg agattatgtt acggaagaag tgcagccgcc
     3241 tccaaccgtt gagcggaaac cggaggtggt gccggtaaat gtgaaaaaga cggatcatcc
     3301 cgaacagccc atcccggatg aagagctggc aaaaagtgtg gataagctac cctcctatcc
     3361 cgggggcacc gaggcattcc aggttttcat agacaagctg agcgcagaca tggtaaagga
     3421 actggacccc gggcagagta aggcatttgt attgatggaa tatattattg atgaaaacgg
     3481 taaaaccatt tatgccaatc ccatcagcgg cggtaatgac cggatgaatg agaagatagt
     3541 gaaagccttc gccggaatgg gtttatggac tcccgccacc cgccagggca aaaatgttcc
     3601 cattaaaatg aagcaaacca tcatggttgg cgttccttag ttcaacggac cagggtttgt
     3661 acggtttgcc gggaccactg ctgcaaaacc atttctttgt ccttgagcag ggactgcaaa
     3721 acttccggcc ggtaatgtct taccgtta
//
LOCUS       DASYCU010000196         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3960163, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000196 DASYCU010000000
VERSION     DASYCU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3960163"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGM24_03925"
     CDS             <1..240
                     /locus_tag="VGM24_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154542.1"
                     /translation="SVSYLHGGAGILRSLQLQLDGLKEGERKQVFLLNEAGGPGDHFK
                     FEVWIENIREALPQELLVGYPLSGAARECAPGCIC"
     gene            268..1329
                     /locus_tag="VGM24_03930"
     CDS             268..1329
                     /locus_tag="VGM24_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4154543.1"
                     /translation="MDIHLLSGFLGSGKTTAIQSACRILSEKGIKAAVITNDQGIRLV
                     DAGLFKQLGIPGRQVLNGCFCCNYRDLDNQIQSLLETAEPEVIFAESVGSCTDIVATV
                     FKPLRVFRPDVRVTLSVFADAMLLHLLLIDKTFLFDEEVNYIYFKQLEESNILIISKM
                     DLLNSDELRDLKNGLNERYPDKLIHYQDSRNDDQVRQWLGQLEPAVSDLNPRSLEMDY
                     AKYGAGEAKLAWLDQELDIESSNKTATRDAIQLMQEIFKKIAAEKHPIGHLKYLLNGQ
                     QKISFTSSSLPEIGFEILPAVGATLLLNARVQTSPGALSLLVSDAIRTFEEQSGCRVR
                     TNSISSFQPGFPTPTCRME"
     gene            1389..2519
                     /locus_tag="VGM24_03935"
     CDS             1389..2519
                     /locus_tag="VGM24_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4154544.1"
                     /translation="MDNESVKMPAYLKLAQILLGIAVFFFILYVGQDIIIPLVFAGMI
                     AILLNPVVLFLCRKKIPRVIAIILSLMVAMLLITGIFYFFISQISLFSESFPQFKHKF
                     LVFFNDSERWVSETFNVSKWKVDAWVTKTQKQSMDNGSGMIGKTVGTIGGVLTTFIIM
                     PVYVFMMVYYKPLLLEFISKLFPKEQHSVVVEVLKETKGLIQNYLLGLLVEALLVATL
                     NSAGLLILGIPYAVLLGVIGALLNVIPYIGGVVAVALPMLLALAVKQPVDALYVLIAY
                     LIVQFIDNNFFVPKIVASKVKINALVSIVVVLIGNALWGIPGMFLSIPLTAIIKVIFD
                     RIKPLEPFGFLLGDNQPDISKQVFMVRRRTRSSGTGGGKKSG"
     gene            2593..>2991
                     /locus_tag="VGM24_03940"
     CDS             2593..>2991
                     /locus_tag="VGM24_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154545.1"
                     /translation="MKAALCIAFSLFVFGCLLPGCGQSPEKTKEIVVMENGNKKVFGV
                     IGRDTVYRYTLQNGKGMKAVVSNYGATLLELWTADRSGSSGDVILGFDSLEGYLQKGN
                     PYFGALVGRYANRIAKAKFSIDGKEYTLAPN"
BASE COUNT          748 a          658 c          764 g          821 t
ORIGIN      
        1 tctgtcagtt acctgcacgg cggtgccgga atactcaggt cccttcagtt gcaactggat
       61 gggttgaaag agggggaaag aaaacaggta ttcttattaa acgaggctgg gggaccgggc
      121 gatcatttca agtttgaggt atggattgaa aacatacggg aagccctgcc ccaggagttg
      181 cttgtcggtt atccgctttc gggcgcagcc agggaatgtg cccccggctg tatctgttaa
      241 gattaaaagg attgtaaaaa gttagccatg gatattcatc tcctgagtgg ttttctggga
      301 agtggcaaga ccacggcaat ccaatctgca tgccgtattt tatcggagaa gggaattaag
      361 gccgccgtta ttacaaacga tcagggcatc agactggtgg atgcaggcct gtttaagcaa
      421 ttggggatac ccggacgcca ggtgctgaat ggttgttttt gttgtaatta ccgcgacctg
      481 gataaccaga tccaatcgct gctggagacc gcggaaccgg aggtgatctt tgccgaatcg
      541 gtcggatcct gcacggatat tgtagcaacc gtttttaaac ctttacgggt tttcaggccg
      601 gatgtacggg taaccctgtc ggtttttgct gatgcgatgt tattgcacct gttattgata
      661 gataagactt ttctgttcga tgaagaagtc aattatatct actttaaaca actggaggaa
      721 tcaaatatcc tgatcataag taaaatggat ctgctgaatt ccgatgagtt gcgggatctc
      781 aaaaacgggc ttaatgaaag atacccggat aagctgattc attaccagga ttcccggaac
      841 gatgaccagg tcaggcaatg gttagggcag ctggaaccgg cagtttctga tttgaatccc
      901 aggtcactgg aaatggacta tgccaaatat ggtgcaggag aagcaaaact ggcctggctt
      961 gaccaggagc tggatataga aagttcaaac aaaacggcca cccgggacgc aatccagttg
     1021 atgcaggaaa tatttaaaaa aattgctgcg gaaaaacatc ctatcgggca cctgaaatat
     1081 ttattaaacg gtcaacaaaa aatcagcttc acctccagct ccctgccgga aatcggtttt
     1141 gaaattcttc ccgccgtagg cgcaaccctg ttgttaaacg cgcgggtgca gaccagtccg
     1201 ggggcgcttt ccctgctggt atcggatgcg atccggacat ttgaagagca gtccggctgc
     1261 cgggtacgga caaatagcat ttcttcgttt caaccgggtt ttccaacgcc aacctgccga
     1321 atggaatagg caatctttgc tgattattcg ggaattcaag tatattgtaa cggacaaaaa
     1381 atcccggcat ggacaatgaa tccgtgaaaa tgcctgctta tctgaaactg gcgcagatcc
     1441 tcctgggcat cgctgttttc ttttttattt tgtatgtagg ccaggacatt attattcccc
     1501 ttgtattcgc cggcatgatc gccattttgc tcaacccggt ggtccttttt ctttgccgga
     1561 aaaaaattcc gcgtgtcatt gccattattt tgtccctgat ggtggccatg ctgctgataa
     1621 ccgggatttt ttactttttt atttcccaga tctcgctctt cagcgaatcc tttccccagt
     1681 tcaaacacaa attcctggtt ttttttaatg atagtgagcg ttgggtttcc gaaacattta
     1741 atgtgtccaa atggaaagtg gatgcctggg taacaaaaac ccagaagcaa agtatggata
     1801 acggatccgg gatgattggc aaaaccgtgg gaacgattgg cggtgtgctg accaccttta
     1861 tcattatgcc cgtgtacgtt tttatgatgg tctattataa accgttgttg ctggaattta
     1921 tttcgaagct ctttccgaag gaacagcatt cggtggtcgt ggaggtgctc aaggaaacca
     1981 aggggttgat ccagaattat ctccttggcc tgctcgtgga ggccttgctt gtggccaccc
     2041 tgaattccgc cggcttactg atcctgggca tcccctatgc cgttttgctc ggcgtgatcg
     2101 gcgcgttatt gaatgttatt ccatacatcg gcggggtggt ggcagtagcc ttgcccatgc
     2161 tgctggcgct tgcggtcaaa cagccggtgg atgcccttta cgtattgatt gcttacctga
     2221 tcgtacaatt tattgacaat aacttttttg ttcctaaaat cgttgcttcc aaagtgaaga
     2281 tcaatgcgct ggtctccatt gtggtcgtcc ttattggaaa tgcgctctgg ggcattccgg
     2341 gcatgtttct ttccattccc ttaaccgcga taattaaagt tatctttgac cggatcaagc
     2401 cattggaacc atttggcttt ttgcttggcg ataatcagcc ggatatttcg aaacaggttt
     2461 ttatggtcag gcgccgtacc cggagttccg gtaccggcgg cggaaaaaaa tccggttaaa
     2521 aagtttgccg cggtgatagt gagtttgcct aattataaaa atgccaactt tacataaaac
     2581 aaaacaaaag ccatgaaagc agcgctctgt attgcctttt cattatttgt ttttggctgc
     2641 ctgcttccgg gctgcgggca gtcaccggaa aaaaccaaag aaatcgttgt tatggagaat
     2701 ggaaataaga aagtattcgg cgtgatcggc cgggatacgg tttaccgcta taccctgcaa
     2761 aacggaaagg ggatgaaggc cgttgtatcc aactacggag ccaccctcct ggaattatgg
     2821 acggcagacc ggtcggggtc ttcgggggat gtgatcctgg gatttgattc cctggaagga
     2881 tatctgcaaa agggtaatcc ctatttcggt gccctggtgg gccgctatgc caaccggatt
     2941 gctaaggcaa aattctcgat cgacggaaaa gaatatacac ttgcaccgaa c
//
LOCUS       DASYCU010000197         8748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3090790, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000197 DASYCU010000000
VERSION     DASYCU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090790"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VGM24_03945"
     CDS             <1..608
                     /locus_tag="VGM24_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEY4154546.1"
                     /translation="RTSLGSDYPIVDLKYTRGIPGVFGSSQQYDKLYASLSDYIKVPP
                     IGSIYYNVFAGKTYGTLPYPFLNLAPGNETYYYDQYAFSLMNKFEYLHDQYLGVNFEH
                     NIGNGLFRFIPFTRKMKFRQFYTIKTLWGNLTDANKSYNSSSDYTFRSLDGKTYMEVG
                     TGVDNIFKILRVDFIWRVLPTDIPATAHERFGVFGSLRLTF"
     gene            complement(635..1879)
                     /locus_tag="VGM24_03950"
     CDS             complement(635..1879)
                     /locus_tag="VGM24_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY4154547.1"
                     /translation="MNQRELFFRHLAQTSATPLALEIVRAEGMYLYDPAGKKYLDLIG
                     GISVCNTGHRHPEVLEAIRKQMDLYLHILVYGELLQSPQIQYAQLLTAHLPASLQSVY
                     FTNSGAEAVEGAMKLAKRVTGRTQIIAFNQSYHGSTQGALSIMGDEYWRNAFRPLLPD
                     VLHLDYNSLDQLENINDHTACVIAETVQAENGVILPANVDPERQKTCGQQKDRRVENR
                     ERQMNWLQAVRKKCDEAGALLVLDEIQVGFGRTGTLWGFEQYGIVPDILLLGKALGGG
                     LPLGAFIAAKKLMDELMDKPVLGHISTFGGHPLSCAAGLASLNVLLKEKLTEGVAEKE
                     KIFQRLLVHPSIRSVRTAGLLIAVAFESFEKNKKVIDSCIQNGLLTDWFLFASNCLRI
                     APPLTISAAEIEKGCAIILDAV"
     gene            1973..2491
                     /locus_tag="VGM24_03955"
     CDS             1973..2491
                     /locus_tag="VGM24_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY4154548.1"
                     /translation="MALILPVNGVSPVFGKNTYLAPNSTIVGDVVMGDDCSIWFNAVV
                     RGDVNSIRIGNKVNIQDGCIIHCTYQKAKTIIGNNVSIGHNAIVHGCILHDNVLVGMG
                     SIIMDGAEVGSNSIIAAGAVVLENTKIEQGVIYAGVPAHKIKDIPQELIQGEIDRIAN
                     NYVLYADWFRDK"
     gene            2719..2907
                     /locus_tag="VGM24_03960"
     CDS             2719..2907
                     /locus_tag="VGM24_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154549.1"
                     /translation="MKAPKNLCLLLAALILFSTGSSCSFVNKTFGPKYGCPANGRNVG
                     AERILSGEKVPKAPKFKA"
     gene            2989..3321
                     /locus_tag="VGM24_03965"
     CDS             2989..3321
                     /locus_tag="VGM24_03965"
                     /inference="COORDINATES: protein motif:HMM:NF023724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3630 family protein"
                     /protein_id="HEY4154550.1"
                     /translation="MSLTLRTDFGCTEAVIDDDCGLKRFYEVANILSDDLDINFNQKT
                     DDFDSLMWDFAFKGHVLTLHYNIYTGISIYPRKFREAPRKDNDAVVEVARFLEGKLLI
                     HTANRLAS"
     gene            complement(3390..4322)
                     /gene="rsgA"
                     /locus_tag="VGM24_03970"
     CDS             complement(3390..4322)
                     /gene="rsgA"
                     /locus_tag="VGM24_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY4154551.1"
                     /translation="MMRAIVYKSTGSWYTVRTEDGKIWNARIKGILKMDGITSTNPVA
                     VGDRVNLDFGDSPEEEAMVTGIEKRNNYINRQSPANKYQHHIIAANLDQSMLIATLKD
                     PKTSQGFVDRFLVTGEAFHIPSIIIFNKSDLYRKKETDLFEERREMYESVGYKVLLVS
                     VRDKTGLEEVEDLLRNKATLLSGHSGVGKSSFINVVVPGLGLKTQDVSDSSGKGIHTT
                     TFAEMFDLPVLNARIIDTPGIREFGLVDISRQELSHYFPEMRIRLNGCQFNNCLHLNE
                     PGCAIKKAVDHGEISLNRYVSYGMIMDSIEEKRG"
     gene            complement(4325..5329)
                     /locus_tag="VGM24_03975"
     CDS             complement(4325..5329)
                     /locus_tag="VGM24_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY4154552.1"
                     /translation="MLYDWFRNIHFAQPLFFALFALIPVLVYWYIHPPFRLQGTMIVS
                     SIRSFGSGRSWKTLLRHALFVFRMIAIVSLITALARPQMGSEQQTTTGEGIDIVLCLD
                     ISGSMLAQDFTPNRMEAAKQVAGEFIDRRPTDRIGLVIFAGESFTMCPLTTDHAVLKN
                     QLFNVQSGLLEDGTAIGSGLATGVERLRSSQSKSKVIILLTDGEDNGGRLDPNTAKEI
                     AKSFGVRVYTVGMGTEGYAPVPVQTSAGIIMQREKVSIDEKLLTQIATETGGKYFRAR
                     DNESLSGIYKEIDQMEKSRFEVKTFTHYTEKFLPFVIVAAAFVLLEWIFRLTVFRKFP
                     "
     gene            complement(5329..6261)
                     /locus_tag="VGM24_03980"
     CDS             complement(5329..6261)
                     /locus_tag="VGM24_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154553.1"
                     /translation="MARPLPNMIRVSGSTLFGVLLMIGLSARAQDSPSVKTSIDKTHI
                     LIGEQIRFGIDVKVPSGSFSSLPQPDTIPHFVFIGNRKLDSLASEGSTSYHLEWKLTS
                     FDSGVNKIPALSVGVGNRKYLSDSLLVEVSYGNLDSAKEYHDIRGIIDIENPAVKYIL
                     WVILGLTAGAILLFFYFGSKPLPPVNVAKEAPASTRLSPYEEALAALAALKKMPLTDA
                     VSVKKYYSVMNDALRIYLSRSLGLRTMERTNEELILQLSNLDMQKEAFTHLAGALRMS
                     DFVKFAKYIPDVYDNERNLEVVRSSIVLINEIRK"
     gene            complement(6265..7143)
                     /locus_tag="VGM24_03985"
     CDS             complement(6265..7143)
                     /locus_tag="VGM24_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY4154554.1"
                     /translation="MLTPLEILKKVRELEIKSKRLTRHIFTGEYHSAFKGRGMSFREV
                     REYYAGDDIRFIDWNVSARFNHPFSKLFEEERELTVMLLVDVSGSSLFGTVKARKKDL
                     ITEICAVLAFSAVNNNDIVGLIFFSDKIERYIPPKKGRDHVLHLVRDLLITEPAHRKT
                     NISEALRFLNNTLRQKTISFLLSDFLDERYEEALRVAGKKHDLIGIKVYDKMDMELPR
                     AGMIEVEDAETGKVEWIDSNDFMQRSYYHQSFFKMTELSKSIFLKSGCDLLHMRTDED
                     YVKVLQKFFVGRNRGR"
     gene            complement(7179..8177)
                     /locus_tag="VGM24_03990"
     CDS             complement(7179..8177)
                     /locus_tag="VGM24_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY4154555.1"
                     /translation="MYTADDIKQLNEKIQYHGLFVDRLREEISHVIIGQQYMLDRLLI
                     GLLSNGHVLLEGVPGLAKTLTIKSLSQTIKGKFSRIQFTPDLLPADVVGTMIYNQQKN
                     EFVVRKGPIFANFLLADEINRAPAKVQSALLEAMQERQVTIGDSSYKLDEPFLVLATQ
                     NPLEQEGTYPLPEAQQDRFLMKVIVGYPSKAEEQLIIRAHMNGMKLPDLNQVVSMQEV
                     LQARDLTRKIYMDEKVEQYILDIVFATRSPEKYRLEKLKPLIAYGGSPRASINLALAS
                     KAHAFLNKRGYVIPEDVRSISKDVLRHRIGLTYEAEAENVNADQVVDDVLKAIVVP"
     gene            8310..8747
                     /locus_tag="VGM24_03995"
     CDS             8310..8747
                     /locus_tag="VGM24_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154556.1"
                     /translation="MMRYSTFLLSAVILFLGFTACKKSDKSNSSRTVANISGTYVLTA
                     LTAGQGGASFNVYDSLPACEKDNKIVLEASGNAQFIDAGPAVCSPSSDSTGSWHLSAG
                     ADSIYLGDEAAYIKSFDGKTLVVTNSQEFSGLTLVATTTLTKE"
BASE COUNT         2169 a         2262 c         2060 g         2257 t
ORIGIN      
        1 accgcaccag cctgggcagc gattatccga tcgtggattt gaaatacacc cggggcattc
       61 cgggcgtgtt tggcagctcg cagcaatacg ataaactgta tgcgagccta tccgattaca
      121 ttaaggtgcc gcccatcgga agtatctatt ataatgtgtt tgccggcaag acctacggca
      181 ccctgcctta tcccttctta aatctggcgc ctggaaatga aacctattat tacgatcagt
      241 atgctttcag tttaatgaat aaattcgaat acctgcacga tcagtatctg ggggtgaatt
      301 tcgagcacaa tatcggcaac gggctttttc gtttcattcc gttcaccagg aaaatgaagt
      361 tccgccagtt ttataccatc aaaacattgt ggggaaacct gacggatgcc aataaatcat
      421 acaacagttc ttccgactat accttccgct cgctggatgg aaaaacctat atggaagtgg
      481 gcacaggtgt ggacaatatc tttaaaatac tgcgtgtgga ttttatctgg cgggttttgc
      541 caaccgatat tccggccact gcgcatgagc ggtttggcgt gtttggaagc ctgagactaa
      601 ctttttaaaa ggcggatgcc atctgcagca aaagctaaac cgcatccaga atgattgcac
      661 agcccttctc aatttctgcg gccgaaatag tcagtggcgg ggcgatccgc aaacagtttg
      721 acgcaaacag gaaccagtct gtcagcaggc cgttctgaat gcaggaatcg atgacttttt
      781 tgtttttttc aaagctttca aacgcaaccg caatgaggag gcccgcggtg cggaccgacc
      841 ggatggaggg gtgaaccagt aagcgctgaa agatcttttc tttttccgca acgccttccg
      901 taagcttttc cttcagcaga acgttcaggg aagccagtcc ggccgcacag cttagcggat
      961 gcccgccaaa ggtgctgata tgtccgagca ccggtttatc catcagttca tccattagct
     1021 tcttcgccgc gataaatgcg ccgaggggaa gtccgccgcc cagtgctttt cccaggagaa
     1081 gaatatccgg aacaatcccg tattgttcga acccccagag agtacccgtt cttccaaatc
     1141 cgacctgtat ttcatccagg acaagcagcg cacccgcttc gtcgcatttt ttccggactg
     1201 cctgaagcca gttcatttgc ctttccctgt tttcaaccct tctgtccttt tgctgcccgc
     1261 aggttttctg cctttcggga tcaacattcg ccgggaggat gaccccgttt tccgcctgta
     1321 ccgtttccgc aattacacag gccgtgtgat catttatatt ttccagctgg tctaatgaat
     1381 tgtagtccag gtgaagcaca tccggcaaaa ggggcctgaa ggcatttcgc cagtattcat
     1441 ctcccataat acttaaagca ccctgcgttg atccgtggta agactgatta aaggcaatga
     1501 tctgcgttct tcccgttacc cgtttggcca gtttcatcgc cccttcaacc gcttccgccc
     1561 ctgaattggt aaaataaacc gactgcaggg aggccggcag atgcgcggtg agcaactgcg
     1621 catattgtat ctgcggggac tgtaacaatt ctccatagac cagaatatgc agatataaat
     1681 ccatctgctt ccggattgcc tccagcactt cgggatgccg gtgaccggta ttgcaaacac
     1741 tgataccgcc aatcaggtcc aggtattttt ttccggccgg atcatagaga tacatccctt
     1801 cggcccgaac gatctccagg gcaagcggcg tagcggaagt ctgtgccagg tgccggaaaa
     1861 ataattccct ctgattcata tataacttgg gctatggagt gaacgctaat gcgtattttt
     1921 gccttgttac gaagataata ccctgtttat tcatcaacgc taaatttcta ccatggcact
     1981 gatcctcccc gttaatggcg tttcgccggt gttcggaaaa aatacctacc tggcacccaa
     2041 ttcaaccatt gtcggcgatg tggtcatggg ggatgattgc agtatatggt ttaatgcggt
     2101 cgtccgggga gatgtcaaca gtatccggat aggtaacaaa gtgaatattc aggatggctg
     2161 catcattcac tgtacatacc agaaagcaaa aaccataatc gggaataatg tttcaatagg
     2221 ccacaatgcc attgtgcatg gttgtattct gcacgacaat gtgctcgtgg gcatgggttc
     2281 catcattatg gacggggccg aggtaggcag caattccatc attgctgccg gggccgtggt
     2341 actcgaaaac accaagatcg aacagggggt aatctatgcc ggcgtgcccg ctcataaaat
     2401 aaaggacatt ccccaggaac tcatccaggg cgagattgac cggattgcca acaactatgt
     2461 gctttacgca gactggttcc gggataaatg agcagcccat cctgctcccg tggttgatga
     2521 gccgtaaata atctccggcg gaacaataaa atacccggaa agatccggct accggaatac
     2581 actaaatccg gagcgattcc gttcatcgta caaatacgca atttcatcga gtttatgccg
     2641 gttccctttg caaatgatga ttcttttttg taggtttaaa ttgttcaatc cggtatctgt
     2701 gaaaacaatt agtaacccat gaaagcgccc aaaaacctct gcctgctcct tgccgcatta
     2761 atcctgttca gtacaggcag ctcctgcagt tttgtaaata aaaccttcgg accgaaatat
     2821 ggttgtcccg ccaacggcag aaatgttggt gcagaaagga tattgagcgg tgaaaaggta
     2881 ccgaaagcgc caaaattcaa agcctgataa acaattttat atccaactat ccctcaatta
     2941 aaacccatga acagcattcc agattataaa cctctaaatc cctccactat gagcctgaca
     3001 ttacgcactg atttcggttg caccgaagca gtcatagacg atgattgtgg cctcaagcgt
     3061 ttttatgaag tcgccaatat tttatcagat gatctcgaca tcaatttcaa ccagaaaacg
     3121 gatgatttcg actcgctgat gtgggacttc gctttcaaag gccatgtact gaccctgcat
     3181 tacaatattt acaccgggat ctccatctat ccgagaaagt tccgcgaggc gccacgcaaa
     3241 gacaacgatg ccgtggtaga agtagcacgc tttctggaag gaaaactgct gattcatacg
     3301 gcaaaccggc tggcctccta gaacccgacc ttagaacaaa cgattccttg ccctccccga
     3361 gggccttttt tttgcccgga cccggctgct tatccccttt tttcttcgat ggagtccatg
     3421 atcattccat aactaacata ccggttcagg gatatttctc cgtgatcgac ggcctttttt
     3481 atggcacagc ccggctcatt caaatggagg cagttattaa actggcaacc gttgaggcgg
     3541 atccgcattt ccggaaaata gtgtgaaagc tcctgtctcg aaatatccac gagcccgaat
     3601 tcgcggatac ccggagtatc aatgatgcgg gcattcaaca caggcaggtc gaacatctcc
     3661 gcaaaggtgg tggtatggat gcctttgccg ctggagtcgc tcacgtcctg cgttttaagc
     3721 cccaggcccg gtaccaccac attgataaag gaagattttc ccactcccga atgcccgctg
     3781 agtaaagtgg ctttattgcg tagcaggtct tcgacctctt ccagccccgt tttatcccgg
     3841 acgctcacca gcaagacctt gtacccaacg gattcataca tttcccggcg ttcttcaaaa
     3901 aggtccgttt cttttttccg gtagaggtcc gacttgttga aaatgataat ggaggggata
     3961 tgaaatgcct cgccggtaac caggaaccgg tctacgaatc cctgcgaggt tttcggatct
     4021 ttcaaggtgg caataagcat cgactgatcc aggttggccg caataatatg atgctggtat
     4081 ttatttgccg gcgactgccg gttgatataa ttattccgct tttcaattcc ggtgaccatc
     4141 gcttcttctt ccggactgtc tccgaaatcc agattcaccc ggtcgccgac ggctaccggg
     4201 ttggtagacg tgatcccgtc cattttgaga atccccttga tgcgggcatt ccatattttg
     4261 ccatcctccg ttctgacggt ataccagctg ccggtggatt tataaacaat cgcgcgcatc
     4321 atgatcaggg aaatttgcgg aagacggtga ggcggaagat ccattcgagc aaaacaaaag
     4381 ccgccgccac gatcacaaag ggaaggaatt tttctgtata gtgggtaaag gttttgactt
     4441 caaacctgga tttctccatc tggtcaattt ccttataaat gccactcagg ctttcattat
     4501 ccctggcccg gaaatatttt ccaccggttt cggtggcgat ctgggtaagc agtttttcat
     4561 caatactcac tttctcgcgc tgcatgatta ttccggcgga agtctggacg ggtaccgggg
     4621 catacccttc cgttcccatt cccaccgtat atacgcgcac tccgaaggat ttcgcaattt
     4681 cctttgccgt attcggatcg agacggcctc cattgtcctc accatctgta agcaggataa
     4741 tcaccttact ttttgactgg gatgatctca gccgctccac tcccgttgcc agtccggacc
     4801 caatggcagt accatcttcc agcaggccgc tctgaacatt aaacagctgg tttttcagaa
     4861 ccgcatgatc cgtagtcagc ggacacatgg taaaactctc gcccgcaaaa atcaccaggc
     4921 cgatccggtc ggttggacgg cggtcaataa attcacccgc gacctgcttg gccgcttcca
     4981 tgcgattggg ggtgaaatcc tgggccagca tgcttccgct gatatccagg caaagcacga
     5041 tgtcaattcc ctccccggtt gtggtctgct gctcactgcc catttgcgga cgcgccagcg
     5101 cagtgatgag ggaaacaatg gcgatcatcc tgaaaacaaa aagcgcatgt cttaaaaggg
     5161 tcttccagga cctgccgctg ccgaaacttc ttatgctgga aacgatcatc gttccctgca
     5221 aacgaaacgg gggatggata taccagtaaa ccagcaccgg gatcaaagcg aaaagcgcaa
     5281 agaacagggg ttgggcaaaa tgaatatttc tgaaccagtc gtacagcact attttcggat
     5341 ttcattgatt aacacaatcg aggagcggac cacttccagg ttccgttcat tatcataaac
     5401 atcgggaata tatttggcaa acttcacaaa atcactcatc cgcaatgcgc ctgccagatg
     5461 ggtaaaagcc tctttttgca tgtccaggtt actcagctga aggatcagtt cttcattggt
     5521 cctttccatg gttcgcagac ccaggctgcg gctcaggtaa atccgcaggg catcgttcat
     5581 gacggaataa tatttcttaa ccgaaacggc atccgtcaga ggcatttttt tcagggcagc
     5641 cagggcagcc agggcctcct cgtaaggaga caagcgcgtg cttgccggcg cttctttcgc
     5701 aacgtttacc gggggaagcg gttttgaccc aaaatagaaa aacagaagga tcgctcccgc
     5761 ggttaatccc aatatcaccc agagaatata tttcacggcc ggattttcaa tatcaatgat
     5821 gccgcgaatg tcatgatatt cctttgccga atccaggttc ccgtagctga cttccaccag
     5881 tagggaatca cttaagtatt tccggttccc gacgcccacg gacaaagccg ggattttatt
     5941 aacgcccgaa tcaaaactgg tgagcttcca ttccagatga taactggtgc tgccttccga
     6001 agccagggaa tccagcttcc tgtttccgat aaacacaaaa tggggaatcg tgtcgggctg
     6061 gggaagcgaa gagaaactgc ctgacgggac tttgacgtcg atgccaaacc ggatctgctc
     6121 cccgatcagg atatgggtct tatctatgct ggttttaacc gaggggctgt cctgggcacg
     6181 ggcggaaaga ccgatcatca ggagaacgcc aaacagggtg cttcccgaca cacggatcat
     6241 attggggagg ggtcttgcca tcgcttaccg gccgcggttt ctgcccacaa aaaacttctg
     6301 aaggactttt acataatctt catctgtccg catgtgcaac aggtcacagc ctgatttaag
     6361 aaaaatcgat ttactaagct ccgtcatttt aaaaaaggat tgatgataat aggaccgctg
     6421 catgaaatcg ttgctgtcta tccattccac ttttcctgtt tcggcgtcct ccacttcaat
     6481 cataccggcg cggggaagtt ccatatccat tttatcgtaa accttgatcc cgatcagatc
     6541 gtgttttttg ccggctacgc gcagggcctc ttcataccgc tcatccagga aatcgctgag
     6601 caggaagctg atggttttct gccgcagggt attgttgaga aaacgaagcg cctcgctgat
     6661 attcgttttc cggtgggcgg gctctgtgat cagcaggtca cgaaccaggt gcaaaacatg
     6721 gtctctgcct tttttcggag gaatataacg ctcgatcttg tcgctgaaaa aaatcagacc
     6781 tactatatca ttattattca cggcagaaaa agcaagcacg gcgcagatct ccgtgatcag
     6841 gtccttcttc ctggccttca cggtcccgaa caaagaactt ccgcttacgt ccaccaggag
     6901 cattacggta agctcccgtt cctcttcaaa taatttactg aacggatggt tgaaccgggc
     6961 agacacgttc cagtcaataa accggatatc atcccctgca taatactccc gcacttccct
     7021 gaaactcatt cctcttccct taaacgcact gtgatactct cccgtgaaga tgtgacgggt
     7081 cagccttttg cttttgattt caagctcgcg gactttcttg agtatttcca gcggagtcag
     7141 catagtatgt ggagtatata atgaatcagg atcgattttt acggaacaac gatcgccttc
     7201 agcacatcat cgaccacctg gtccgcattc acattttctg cttccgcttc gtaggtaagg
     7261 ccgatccggt ggcgcaaaac gtcttttgaa atggagcgca cgtcttcggg aatcacatat
     7321 cctctcttgt tcaaaaacgc atgggccttg gaagccaggg ccaggttgat gctggcgcgg
     7381 ggcgaaccgc cgtaggcaat caggggtttc aatttctcaa gccggtattt ttccgggctg
     7441 cgggttgcaa aaacaatatc cagtatatat tgttccactt tttcatccat gtatatttta
     7501 cgggtcaggt cccgggcctg gagcacttcc tgcatagaaa ccacctggtt caggtccggg
     7561 agtttcatac cgttcatgtg cgcccggatg atcagttgtt cctcggcttt gctgggatag
     7621 ccgacgatca ctttcatcag gaagcggtcc tgctgggcct ccggcagggg atacgttcct
     7681 tcctgctcca gcgggttttg tgtggccagc accaggaaag gctcgtccag tttgtagctg
     7741 ctgtcgccaa tcgtcacctg tctttcctgc atggcttcca gcagggcgct ctgcactttt
     7801 gccggggccc gattgatctc atcggccagg aggaaattgg cgaaaatcgg tccctttcgc
     7861 accacaaatt cattcttctg ctgattataa atcatggtgc cgaccacatc ggcaggcagc
     7921 aggtcggggg taaactgaat acggctgaat tttcccttga tggtttggga aagggattta
     7981 atggtgaggg tcttcgccag gccgggaacg ccctccagca gcacatggcc attgctgagc
     8041 aatccgatca ggaggcggtc cagcatatat tgctgtccga tgatcacatg ggaaatctct
     8101 tcccggagcc ggtcaacaaa taatccatga tactggattt tttcattaag ctgtttaata
     8161 tcatccgccg tgtacatgtt gataaaaaat taggctgcaa aatacgaatt cgttaattag
     8221 aaccccctgc cttgttaaaa cttcggaagg gtgcacaata aattttaaca tacatagtta
     8281 caagctatta tttcattaaa actttgatta tgatgcgtta ttcaacgttc ctcttatcag
     8341 cagttattct tttcctgggg tttaccgcct gcaaaaaaag cgataaatca aacagttcca
     8401 gaacagttgc caatatttca ggaacttatg tcctcactgc tttaacagca ggccagggag
     8461 gcgcttcgtt caatgtatat gattccctgc cggcttgtga aaaggacaat aagatcgtac
     8521 tggaagccag cggcaatgcc cagtttatag atgcggggcc tgcggtctgc agcccgtcca
     8581 gcgattccac cggttcctgg cacttgtcgg ccggcgcaga ttccatctac ctgggggacg
     8641 aggctgcata catcaaatcc tttgacggaa aaacgctggt cgtaaccaat tcgcaggaat
     8701 tcagcggact gaccctggtt gccaccacta cgttaacaaa agaataag
//
LOCUS       DASYCU010000198         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3670736, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000198 DASYCU010000000
VERSION     DASYCU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3670736"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..>1650
                     /locus_tag="VGM24_04000"
     CDS             179..>1650
                     /locus_tag="VGM24_04000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY4154557.1"
                     /translation="MRICFLIAGTLLLTGACNTTEKKEPPDFLAADLDTAVSPGADFF
                     DYANGGWLKRNPIPADHSSWGIGQMVDLDIYNRLRTINEKAAAEQAAPGSTAQKIGDF
                     WKSGMDSAGIEKQGLTPLEPDLIRIGKIASVRDLIQVAADLHRKGVQVLLADEISQDE
                     KYSDQMAYHLGQGGLGMPDRDYYFNTDEKTVQVRKSYRHYLFNAFRTLGKDSSEAMKA
                     AEGVYQLETRLAKASRKIAALRDPDSNYHKMGIGPLQKLCFRIDWKDYLKNTGIASID
                     SLIVGQPEFLEALNRELFQTPLEVWKNYLAFHLLNHSAYYLDTQIYMDRFNYTRAITG
                     VEIPRPRWKRVLDAEQDAIGEALGQLFVKEYFPEKARQRYRDLVESIRNAYKERIRKL
                     TWMSDSTRQNALYKLSRITEKVGYPDKWRDFSALRIDQGPYVLNAQRAAEWWHNDMIK
                     KLGKPVDRTEWDVTPQTYNAFYNPSNNEIVLPAGIFIVPG"
BASE COUNT          474 a          397 c          437 g          342 t
ORIGIN      
        1 cccagctgga acgcgccatc acggaaataa aaacaaggat aaaagaaaat ggatttacgc
       61 cgcctgccag accggcttat gaaaaaagaa attaaaaccg gaaatcagtc ctgggcgttc
      121 ccggaaatga tggaggaaag aaaggagaat acgttagaaa aattaataaa cagcatctat
      181 gcggatttgc tttttaattg ccggaaccct gctgctcacc ggagcctgta atacaactga
      241 aaaaaaagag cctccggatt ttcttgcggc agacctggat acggccgtca gccccggcgc
      301 ggactttttt gattatgcga atggcggctg gttaaaaaga aatcccatcc cggcggatca
      361 cagttcctgg ggaatcgggc aaatggtgga tctcgatatt tacaaccgcc tccggaccat
      421 caatgaaaaa gccgcagcag agcaggctgc gccgggcagt accgctcaga agatcggtga
      481 tttctggaag agcggaatgg attcggccgg tatcgaaaag cagggattga ctcccctgga
      541 gccggatctg atccggatcg gaaaaatagc ctctgtcagg gacctcatac aggtcgcggc
      601 ggaccttcac cggaaagggg tccaggtgct cctcgcggat gaaatttccc aggacgaaaa
      661 gtatagcgac cagatggcct atcatctggg gcagggaggg ttgggcatgc ctgaccggga
      721 ttattatttc aatacggatg aaaaaacagt ccaggtcaga aaatcctacc gccattacct
      781 gttcaatgct ttccggaccc tgggtaaaga cagttcagaa gcgatgaaag cagcagaagg
      841 agtttatcag ctggagaccc ggctggcaaa ggcttcccgg aaaatagccg cgctgcggga
      901 tccggattca aattatcata aaatgggcat tggccctctt caaaaattat gcttccgcat
      961 cgactggaaa gattatctga aaaatacggg cattgcatcc atcgattcgc tgatcgtcgg
     1021 acagccggaa ttcctggaag ccctgaaccg ggagttgttc caaacccctc ttgaggtctg
     1081 gaaaaattat ctggcctttc atttactgaa tcattcggcc tattatctgg atacgcagat
     1141 ctacatggac cggttcaact acacgcgcgc cattaccggc gtcgaaattc caagaccgcg
     1201 ctggaagaga gtcctggatg cagaacagga tgccattggc gaggccctcg gacaattgtt
     1261 cgtcaaggaa tattttcctg aaaaagcaag acagcgttac cgggacctgg tggagtcgat
     1321 ccggaatgca tataaggaaa ggatccggaa actcacctgg atgagcgaca gtacccggca
     1381 aaatgccttg tataaacttt cccggatcac ggaaaaagtc gggtacccgg ataaatggag
     1441 ggatttcagc gcactgcgta tcgaccaggg tccctatgtg ctgaatgccc agcgggctgc
     1501 ggaatggtgg cacaatgata tgatcaaaaa gctgggcaaa ccggtggaca gaacagaatg
     1561 ggacgtcacg ccacaaacct ataacgcctt ttataatccc agcaacaacg agattgtatt
     1621 accggccgga atcttcatcg ttcccggtaa
//
LOCUS       DASYCU010000199         4976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2800399, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000199 DASYCU010000000
VERSION     DASYCU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800399"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1058)
                     /locus_tag="VGM24_04005"
     CDS             complement(192..1058)
                     /locus_tag="VGM24_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4154558.1"
                     /translation="MDKKLLLLAYAFFGLIPSGRSAVKFPEADTVKRMLVTYDLEYGR
                     KLNDRQQAEVLTLDVYEPRDDRTQKPVIILVHGGGFGSGDKQEDLFVKMATAFAGHEY
                     LVFVVNYRLKGPGIPCGQEVLERAVSDVADAVNWIKKNRKRFKADTSRMIICGDSAGG
                     AIAVNLCFDQARDRTFAGCIDLWGGMCNPPGPGYAHRLPWSVPVYEKQLSGEIPPVCI
                     IHGTSDEVVPYSTSTELSAQLSSKGIYNELHSLEGARHFPEQKAVEFIPVMIAFSDKV
                     ISGKANKNHSSL"
     gene            complement(1166..2836)
                     /locus_tag="VGM24_04010"
     CDS             complement(1166..2836)
                     /locus_tag="VGM24_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154559.1"
                     /translation="MKSKIYISGAVLGSLLMAACTKLDREFVTTLSQQQVTQNFTYTS
                     NRAAAVYTHLPLGFLYIDGAMMASATDEAEHTPDTSTIQNFNSGNWNATANPDDVWAK
                     YYQGIRDANLFLESAGNVNLDQWKLNPALQATYQTNLDLIKKWEYEVRFLRAYFYFEL
                     VKRYGGVPLLTSSLAISDTGKTRASLADCIKFMTDECDSAALNLPATPANTDLGRATK
                     GAALALKSRVLLYAASDLFNTPSGSSPALVSIVGGDRMAQWKAAADAAKAVIDLGTYT
                     LSPDYSTLFSSTNYRNNEVIFATRSGPANTFEIASYPIGFDLGQSGTTPTQNMVDAYE
                     MTDGTPFDWNNPTESADPYSNRDPRLGMSVITNNSMFKSRPVEIWAGGRDGKGVTNAT
                     KTGYYLRKYVNPDLDLLLNTTSVHTWILIRLGEIYLNYAEALNEYDPGNPDIKLYVDA
                     VRARPGVNMPGLPGGLSQDQMRDRIRNERRVELAFEDHRFWDVRRWMIGPITEGAPIT
                     GVDIQQTAPSSFTYTKVHIEDRYFDPKMYLYPIPQGEVANGLIQNPLW"
     gene            complement(2865..>4976)
                     /locus_tag="VGM24_04015"
     CDS             complement(2865..>4976)
                     /locus_tag="VGM24_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154560.1"
                     /translation="DVDWYNEVMKKTTMISNYNLNLSGGNNDVRYFVDLNLLNNNGLF
                     QDNSVAAKNGMNSVYYRNYNFRSNLDINLSKRLTATFNLSGAITDKSNEAAATTAPVF
                     ANLDAIAPNEFPVYNPDGTYGGSGLHANPKGDLIETGLYSTNAKALSVNLKLTEQLDM
                     ITKGLSISAAGAFNNFSRSISTKTRQYERYALAKDSAGNASYNKFGQKTSLAGTETSD
                     AQWQNYSIQGYLNYDRTFGPHEINAMLMYSTTVYSASGVSDDFKHKGTAGRFTYNYLK
                     KYIAEFSFGHIGSENFPDGKRYGFFPAGSLAWIVSNEGFLKGNNTLSYLKIRGSYGLT
                     GNDNIGGQRFPFYSYYTSVPAYYLGTNNAATTAIGEGITPNPDVTWEREKQLNLGIEG
                     TLFKQLEFSLDWFKKDRYDILATPYRDVPLYLGLNLPTLNVGKVTNKGLEAMLRFNSK
                     ETGAFQYFIEANVWVARNKIEYNSEAYQTNSYLYRTGHPIGQPFLLQATGFFKDQADI
                     DASPKQVFDNVRPGDIKYKDQNNDGVINANDMYPIGKTDLPELTATLQAGVKYKGLYL
                     NFLLWGVSGRSVYLGSNYFKAFQNNGKISSFALNRWTPETAETANYPRLSSVNNLNNF
                     QPSSFWQRNGDFLKLRSIELGYNLTPAMFRKIGIDHARVFVNGNNLFSIDGANYTDLE
                     IMTGTGYPAVRTISVGANLFF"
BASE COUNT         1169 a         1244 c         1198 g         1365 t
ORIGIN      
        1 agtgcggctg atcacccctt tcgctgaact gatctcccgg tcaatgcccg gggcgcatcc
       61 ttgcaggaaa aaggaaaacc caattaaaag aacggctatg aacaacggat tcttcctgtc
      121 tgtttttgaa atggcgcaat ctttcattgt ttctttattg ctgactgttt tcatatctga
      181 atccggaact attacaggga cgaatggttt ttatttgctt ttccggagat cactttgtcg
      241 gaaaaggcaa tcatcaccgg aataaattca acggcttttt gttccgggaa atgacgtgct
      301 ccctccaggg aatgcagttc gttatatatc cctttgctgc tgagctgcgc cgacaattcc
      361 gtgctggtag aataaggaac cacttcgtcc gaggtcccgt gaatgatgca gaccggcgga
      421 atttctccgg ataattgttt ttcatatacc ggtacactcc agggaagccg gtgagcgtat
      481 cccggtcccg gcggattaca cattccgccc cataaatcta tacagccggc gaacgtacgg
      541 tccctggcct gatcaaaaca caaattcacg gcgatggccc caccggcaga atcgccgcaa
      601 atgatcattc tcgaagtatc cgccttaaaa cgtttccggt ttttctttat ccagttcacg
      661 gcgtccgcga catcggacac tgccctttcc agtacttcct gaccgcaggg aatacccggg
      721 cctttcaacc ggtaattgac tacaaaaaca aggtactcgt ggcccgcaaa tgcggttgcc
      781 attttcacaa acagatcttc ctgcttatca ccgcttccaa agcctcctcc gtgcacgagg
      841 ataatcaccg gtttttgtgt ccggtcatcc ctgggttcat acacgtcaag cgtcagaacc
      901 tccgcctgct gacgatcgtt cagcttgcgg ccgtattcca gatcataggt caccagcatg
      961 cgctttacgg tatccgcttc cggaaatttt acagcagacc ggcctgaagg gatcaaaccg
     1021 aaaaacgcat acgccaggag caacaatttt ttatccatag aaatagttac agatgcttat
     1081 cggtcatggt gcgtttcaaa tggatagtcc ctcctttccg ataccttctg ataacggtat
     1141 tgtatgtaag gagcccttta aatgattacc aaagaggatt ttgaatcaag ccattggcaa
     1201 cctccccctg tggtattgga tagagataca ttttaggatc aaaatagcgg tcttcaatat
     1261 gtactttggt ataggtaaag ctggagggtg cggtttgctg gatatccaca cccgtaatgg
     1321 gagccccttc ggtgattggt ccgatcatcc acctccgcac atcccagaag cgatggtctt
     1381 caaaggccag ttcaaccctt ctttcattgc ggatccggtc ccgcatctgg tcctgtgaaa
     1441 ggcctcccgg caatcccggc atattcacac ctggcctggc acgaaccgca tccacgtaca
     1501 gtttgatatc gggattgccc gggtcatact cattcagcgc ttccgcataa ttcagatata
     1561 tttcacccag gcgaataagg atccaggtat gcacactcgt ggtattgagc agcaggtcca
     1621 ggtccgggtt aacatatttt ctgaggtagt atccggtctt cgtggcattg gtgactcctt
     1681 tgccgtctct gccgcccgcc cagatttcca caggccttga cttgaacatg ctgttattcg
     1741 taatgacact catgccgagt cgcggatccc tgttggaata cggatctgcg gattccgtag
     1801 ggttgttcca gtcgaacgga gtcccgtccg tcatttcata ggcgtccacc atattctggg
     1861 taggcgttgt accactttgt cccagatcaa atccgatggg ataagaagcg atttcaaagg
     1921 tattggcagg tccgctccgg gtggcaaaaa tgacttcgtt attccggtaa ttggttgatg
     1981 agaacaaagt gctgtaatcg ggactcagcg tataggtgcc gaggtcgatc acggccttgg
     2041 ccgcatccgc tgctgccttc cattgtgcca tccggtcgcc acccacgatc gataccagcg
     2101 ccggactgga tccggaaggc gtattaaata aatcactggc tgcatagagt aagaccctgc
     2161 ttttcaatgc cagtgctgca cccttcgtgg ctctgcctaa atcggtgttg gccggcgttg
     2221 ccggcaggtt caatgccgcg gaatcacatt catccgtcat aaactttatg caatcggcga
     2281 gggaagcccg ggttttgcct gtatcactta ttgccaggga agaggtcagc aggggaacac
     2341 cgccatagcg ctttacgagt tcgaaataga aataagctct caggaaacgg acttcgtatt
     2401 cccatttctt tatcaggtcc aggttggttt ggtaggtagc ctgcagcgcc ggattcagtt
     2461 tccactggtc caggttgaca ttccctgccg attcaagaaa caggttggca tcccgtattc
     2521 cctggtaata tttcgcccac acgtcatcgg gattggctgt tgcattccag tttccggaat
     2581 taaaattctg tatggtggag gtgtcgggcg tatgttccgc ctcgtcggtg gccgacgcca
     2641 tcatcgctcc gtcaatataa agaaatccca agggcagatg ggtatatact gctgctgcgc
     2701 ggttggacgt gtatgtaaaa ttttgggtga cctgctgttg ggacagggtg gttacaaact
     2761 cccggtctaa tttcgtgcag gcggccatta gaagcgaacc gagaacagcc cctgatatat
     2821 atattttact tttcataaac aatgttgttt tgattatcta ttctttagaa aaataaatta
     2881 gctcctacac tgatggtcct caccgcaggg tagccggtac cggtcattat ttccagatca
     2941 gtataatttg caccgtctat tgagaaaaga ttgtttccat tgacaaaaac cctggcatgg
     3001 tcgatcccga tcttcctgaa cattgccgga gttaaattat atcccagttc tatgctgcgc
     3061 agtttcagga aatcgccgtt gcgctgccag aaagaggaag gctgaaaatt attcaggtta
     3121 ttcacgctgg acagccgggg ataattcgcc gtctctgccg tttcgggcgt ccaccggttc
     3181 agggcgaagg aggagatctt gccgttgttc tgaaatgcct tgaagtaatt acttcccagg
     3241 tatacggagc ggccgctcac tccccacaaa aggaagttca gatataagcc tttatacttg
     3301 acccctgcct gcaaggtggc cgtcaattcg ggaagatctg ttttaccgat tggatacatg
     3361 tcattcgcat ttatgacgcc gtcattgttc tgatccttgt atttgatatc ccccggtctt
     3421 acattgtcaa agacctgctt cgggctggcg tcaatatctg cctgatcctt aaagaatccg
     3481 gtggcctgca gcaggaaggg ctgaccgatc ggatgtccgg tgcggtacag gtaactgttt
     3541 gtctggtatg cctcagaatt atattcgatc ttgtttcggg ctacccacac attggcttcg
     3601 ataaaatact ggaaagcacc ggtctcttta ctgttaaacc gcagcatggc ttccagtccc
     3661 ttattggtca ccttacccac gttgagcgta ggaagattca gtcccaggta caggggcacg
     3721 tcgcggtaag gcgttgccag gatatcgtaa cgatcttttt taaaccagtc gagtgaaaat
     3781 tccagttgtt tgaaaagggt tccttcaatg cctaaattca actgcttctc cctttcccag
     3841 gttacatccg ggttgggcgt tattccttcg ccgatcgcag tggtggcggc gttattggtt
     3901 cctaaataat aggccggcac cgaagtgtag taagaataga agggaaagcg ctggcccccg
     3961 atattgtcat ttccggtaag gccgtaagaa ccccttattt tcagatagct gagcgtgtta
     4021 ttccctttaa gaaaaccctc gtttgaaacg atccatgcca gcgaaccggc cggaaaaaag
     4081 ccgtagcgct tgccatccgg aaaattttca gatccaatat gaccaaagga gaactctgcg
     4141 atgtattttt tcagatagtt gtacgtgaac cggccggccg tccctttatg cttaaaatca
     4201 tcgctgactc cgctggcgga atataccgta gtcgagtaca tcagcatcgc attgatctca
     4261 tggggaccga atgtccggtc atagttcaga tagccctgta tcgaataatt ttgccattgg
     4321 gcgtctgagg tttcagtgcc cgccagggag gttttttgac cgaatttgtt ataggaggcg
     4381 ttccctgcgg aatcctttgc cagtgcatat ctttcgtact gtcttgtttt tgtggatatg
     4441 ctccttgaaa aattattgaa tgctcctgca gcggatatgc ttaacccctt cgtgatcatg
     4501 tccagttgct ccgtcaattt cagattcacg gaaagggctt tggcattggt gctgtaaagg
     4561 ccggtttcaa tgagatcgcc cttgggattg gcgtgcagcc cggatcctcc gtaggttccg
     4621 tcgggattat ataccggaaa ttcattggga gcaatcgcat ccaggttggc gaagaccggc
     4681 gcagtggtcg cggcagcctc attggattta tctgtgatag ccccggacaa attgaacgtc
     4741 gccgtcagtc tcttcgataa attgatatcc aggttggaac ggaaattata attccggtaa
     4801 taaacagaat tcataccgtt cttcgcggca acggaattgt cctgaaacaa tccgttattg
     4861 ttcagcagat tcaggtctac aaaatacctg acatcattgt taccaccgga taagttcagg
     4921 ttataatttg atatcatggt cgtctttttc attacctcat tataccagtc cacatc
//
LOCUS       DASYCU010000200         6275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4104184, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000200 DASYCU010000000
VERSION     DASYCU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104184"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGM24_04020"
     CDS             complement(<1..477)
                     /locus_tag="VGM24_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEY4154561.1"
                     /translation="MNTALPRKGHPRGLYVLFFTEMWERFGYYLMVGILFLFLHDTVN
                     NGGRGLDTGLSADIVGTYIALVYLTPFIGGLIADRYIGYRISVFLGGSLMALGYFLMA
                     VPHSGGLMYAALASVIIGNGFFKPNISTLLGNIYNKEELKPKKDIAYNIFYMGINIG"
     gene            634..1911
                     /gene="purD"
                     /locus_tag="VGM24_04025"
     CDS             634..1911
                     /gene="purD"
                     /locus_tag="VGM24_04025"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY4154562.1"
                     /translation="MNILLLGAGGREHAFAWKISESPDCSRLFIAPGNAGTSRFGTNI
                     EIGPTDFHAVKKACLENGINLLVVGPEEPLVNGISDFFATDPELSSIPVIGPSAAAAR
                     LEGSKAFSKRFMVKHGIPTAAYREFDMENYLEGSRYLESHGLPIVLKADGLAAGKGVV
                     ICKSHVEAIAEFELMLQQNKFGEAGKRIVVEEFLDGIELSVFILTDGDHYVLLPEAKD
                     YKKIGEGDKGPNTGGMGAVSPVPFADETFMNKVKERIIEPTIRGLKEEQIDYRGFIFI
                     GLIKVNEEPVVIEYNCRMGDPETEVVLPRLQNDLVALFRAVADKKLDEITINIDPRVT
                     ATMVAVSGGYPGSYEKGFPITGLDYAEADSLIFHSGTRQENGKVLTNGGRVLCVTAYA
                     QSLPDAIRKSMRVLQHIDFDGMYYRKDIGYEFL"
     gene            1993..2985
                     /locus_tag="VGM24_04030"
     CDS             1993..2985
                     /locus_tag="VGM24_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY4154563.1"
                     /translation="MESPLHYADYLKLDTVLHAQQLRSDELNAHAHDEMLFIITHQTY
                     ELWFKQLLYEVDSVKAILQKPALNDDSPELQIIVHRLNRSNVILKVLVQQIGIMETMS
                     PMDFLDFRDMLRPASGFQSWQFKLLEAKLGLTFGQRHGQAHYISQLRESEVKLIREAE
                     SGKTLLELLNAWLERMPFLKDNDFWPGYRKLYLESLSETEMANAQLFDTVFSEDPGNS
                     LRSLSPAASRAGLFIMIYPGYPILQLPFQLLNQLLEMDEQLSTWRYRHMHMVHRTIGT
                     RIGTGGSAGRDYLQGTLEKHFIFRDIAQLTSFLIERKKLPQLSREMEKRLGFAS"
     gene            3139..4164
                     /locus_tag="VGM24_04035"
     CDS             3139..4164
                     /locus_tag="VGM24_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY4154564.1"
                     /translation="MGLFNFLTQEIAMDLGTANTLIIHNDEIVVNEPSIVALDRNNPK
                     TVLAVGKRALMMHEKTHESIRTVRPLKDGVIADFNAAELMIREMIKMIYPKKPLFPPS
                     WRMMICIPSSITEVEKRAVRDSAEQAGAKEVYLIHEPMAAALGIGIDVEEPVGNMIID
                     IGGGTTGITVIALAGIVCDQSIRIAGDEFTADIMEALRRYHSLLIGERTAEQIKIQIG
                     AALKDLDNPPDDIPVNGRDLVTGIPKQILVSYQEIAEALDKSIFKIEEAILKALETTP
                     PELAGDIYRRGLYLTGGGALLRGLDKRLSQKIKLPVHIADDPLKSVVRGTGISLKNYD
                     RYPFVMR"
     gene            4205..5059
                     /gene="mreC"
                     /locus_tag="VGM24_04040"
     CDS             4205..5059
                     /gene="mreC"
                     /locus_tag="VGM24_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY4154565.1"
                     /translation="MRNVFLFIRKYANFLFFLVLQIIALSFLFRYNKYHEAIFMNAAG
                     EITGSLDQRYNTVEYYFKLKKINEQLAAENVRLNQALKENYERPEAGAKIVLDTVQID
                     SLRQIQKYSWLGARVVGSTVSTQTNFITIHRGSNQGVLPNMGVVSPLGIVGTVVNVSE
                     NFASVMTLLHRQYKVVVKMKNSGDRGTVEWDGISPSYITLKDIPKSAKVQKGDSVVTS
                     PTSSLFPGNIMVGIVEEIAEEKSSNFYTLKLRPATNFFNIEYVYVISNSQLVEQKRME
                     DSTHKKFQ"
     gene            5056..5589
                     /locus_tag="VGM24_04045"
     CDS             5056..5589
                     /locus_tag="VGM24_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY4154566.1"
                     /translation="MSELLKNIIRFILFIFVQVFILFKMPPLHRFISPYLYYLYILWL
                     PFSMSRAGLMSVAFLFGLTLDYFMHTPGLHAAACVLIAYLRPFVVNMLISQEGADKNY
                     LSPSITSMGWAPYGTYVFILTLFHHGYLVFLEWLSFGSFLYFIGKVIATTGISMLLIL
                     LTELLFFRKEKFRTNTV"
     gene            5611..>6275
                     /locus_tag="VGM24_04050"
     CDS             5611..>6275
                     /locus_tag="VGM24_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY4154567.1"
                     /translation="MPVFNQSRSNIIRLIFAGMFLIIFAQLFNLQVISDKYKKMAQEN
                     ALFPKRIYPTRGIIFDRHNRAILNNTIMYDLMVTPSQVKNMDTAYFCQLMGIDSAEFR
                     SRVLDARIRNGPFRPTIFEDLLPPDLYAKLEENLWRFPGFNLQERPVRVYPFDAGAHI
                     MGYVGEVDSNILRRSKFYYQLGDYVGRSGLEQFYEKQLMGQRGIEYWIKDNKNRLVGH
                     YANR"
BASE COUNT         1642 a         1506 c         1588 g         1539 t
ORIGIN      
        1 tccgatattg attcccatgt aaaagatatt gtaggcaata tctttcttcg gcttcaattc
       61 ttccttgtta taaatattgc cgaggagcgt gctgatattg ggtttgaaaa aaccgttccc
      121 gataatgacg ctggccaggg cggcgtacat cagtccgccg ctgtgtggta cggccatcag
      181 gaaataaccc agcgccatca gcgatcctcc gagaaaaacg gatatccggt acccgatata
      241 ccggtccgcg atcagtccgc cgataaaagg agtcagataa accagggcaa tataggttcc
      301 tacaatatcg gcggaaagcc ctgtgtccag gcctcttcct ccgttattca cggtgtcatg
      361 caaaaataga aacaggatgc cgaccatgag gtagtaaccg aagcgttccc acatttccgt
      421 aaaaaacaat acatataaac cccgcggatg tccttttcgt ggcaaggctg tattcatatg
      481 tgtaagttta gagttgaggt tgacctgaat taaaggaccc taatgtaaga tttatttatt
      541 catcacgcgg tacggagctt tcctgccatt cataaaatta ttttttcaga tatggtctcg
      601 aatcgtgaat ttcgggccta atcggctgta caaatgaata tattattgct aggagcggga
      661 ggaagagagc atgcctttgc ctggaaaata tcggaaagcc cggattgttc ccgtttattc
      721 attgccccgg gtaatgcggg aacttcccgt ttcgggacaa atattgaaat cggcccgacg
      781 gatttccatg ccgttaaaaa agcctgcctt gaaaatggca ttaacctgct ggtcgtgggt
      841 ccggaagagc ctctggtaaa tggcatttcc gatttttttg ccacggatcc ggagctcagc
      901 tccatcccgg tgatcggacc ttccgccgcc gcggcgcggt tggagggaag taaggcgttt
      961 tcgaaacgat tcatggtaaa gcatggtata ccgacggcgg cctaccgcga atttgacatg
     1021 gagaattatc tggaaggctc cagatatctg gaatcccacg gccttcccat cgtgctgaaa
     1081 gccgacggac tggcagccgg caaaggggtg gtcatttgca agagccacgt ggaggcgatc
     1141 gctgaatttg aactgatgct gcaacagaat aaattcgggg aagccggcaa acggattgtg
     1201 gtggaagaat ttctcgatgg tatcgagttg tctgttttta tactgactga cggggaccat
     1261 tatgtgcttt tgccggaagc taaagactat aagaaaatcg gggaaggcga taaaggcccg
     1321 aacaccgggg gaatgggggc ggtcagtccc gtgcccttcg cagatgaaac ctttatgaac
     1381 aaggttaaag aacggattat tgaaccgact atccgcgggc tgaaggaaga acagattgat
     1441 tacaggggct ttatttttat cgggttgatc aaggtaaatg aagaacccgt ggttattgaa
     1501 tacaattgcc gcatggggga tcccgaaacg gaagtggtgc ttccccgcct gcagaatgac
     1561 ctggtcgccc tgttccgggc ggtggccgat aaaaagctgg atgaaattac cataaatata
     1621 gatccccggg tcaccgccac catggtggcg gtcagcggag gatacccggg aagctatgaa
     1681 aagggatttc ccatcaccgg cctggattat gccgaggcag acagcctgat ctttcattcc
     1741 ggaacaaggc aggagaacgg gaaagtactg acgaacgggg ggagggttct atgtgtaact
     1801 gcttatgcgc agtctttgcc ggatgccatc cggaaatcca tgcgcgtcct tcagcatatt
     1861 gatttcgacg gcatgtatta ccggaaggac atcggatatg aattcctctg atccgccggg
     1921 ccgggcggtg atcgcgccgg cagaaaagga atggggatgc gatttgcatt ccggtaacct
     1981 aacttcgcag ggatggaatc cccgcttcac tatgccgatt acctaaagct ggatacggtt
     2041 cttcatgccc agcagctccg gagtgatgag ctgaatgccc atgcccacga tgaaatgctc
     2101 tttattatca cccaccagac ctacgagctt tggttcaagc aattgcttta tgaagtggat
     2161 tccgtgaaag ccattcttca gaaacccgcg ttgaacgacg attcacccga attgcagatc
     2221 attgttcacc ggctgaaccg cagcaatgtg atcctgaagg tactggtaca gcagatcggt
     2281 atcatggaaa cgatgtcccc gatggatttc ctggatttcc gggacatgct ccgtcccgct
     2341 tccggttttc aaagctggca gttcaagctg ctggaagcta aactggggct gacgttcggg
     2401 caaagacacg ggcaggcaca ttatatttcg cagctgcgtg aatccgaagt aaagctcatc
     2461 cgggaggccg aatccgggaa aaccctgctg gaattactga atgcctggct ggagaggatg
     2521 ccgttcttaa aggacaatga tttctggccc gggtaccgga agctctatct ggaaagtctt
     2581 tcggaaaccg aaatggcaaa tgcgcagtta ttcgataccg tattttccga agaccccggc
     2641 aattctttgc gatctctttc cccggctgcc agccgcgcgg gcctatttat catgatttat
     2701 cccggatatc ccatccttca gttacctttc caactgttga atcagcttct ggaaatggat
     2761 gaacaactga gcacctggcg ttaccggcat atgcatatgg ttcaccggac catcggaacc
     2821 cgcatcggaa cgggaggaag cgctggccgg gactacctgc agggcacact cgaaaaacat
     2881 tttatctttc gcgatattgc ccaactcacg agttttctca ttgaaagaaa aaaactgcct
     2941 caactgagca gggagatgga aaaacgcctg ggtttcgctt cataataaag atttattttt
     3001 aggaaatatc cgcgtgaaaa tagcgttcag tgcaggatat ccacgatatt ataaatcagg
     3061 gatgttcact ttgttaattc ggattatttc catcaatttt gcatgcatcg taacagaatt
     3121 taaatctgac cttttaaaat gggattattc aatttcctga cgcaagagat tgctatggat
     3181 ctggggacag ctaacaccct gatcattcac aatgatgaaa tcgtagtaaa cgaacccagc
     3241 atcgtagccc tggaccggaa taatccgaag accgtactcg ccgtcggaaa aagggcgctc
     3301 atgatgcatg aaaaaacgca tgaaagcatc cggaccgtcc gccccctgaa agacggggtg
     3361 atcgccgatt tcaatgctgc cgagctgatg atccgggaaa tgatcaagat gatctatccc
     3421 aaaaaaccat tattcccccc gagctggcga atgatgatct gcattccctc cagtattacc
     3481 gaagtggaaa aaagagcggt gcgcgacagt gccgaacagg caggggccaa agaggtttac
     3541 ctgatccatg aaccaatggc cgcggccctg gggatcggga ttgacgtgga agagcccgtg
     3601 ggaaatatga tcatcgatat tgggggcggc accaccggca ttacggtaat tgccctggcc
     3661 gggatcgtct gcgaccagtc tatacgcata gcaggagatg aattcacggc cgatatcatg
     3721 gaagcccttc gccgctatca cagtttattg attggcgaaa gaacggccga acagatcaag
     3781 atccagatcg gcgcggcact gaaagacctg gacaatccgc cggatgatat tccggtgaat
     3841 gggcgtgacc tggtcaccgg catacccaag cagatcctgg tgagttatca ggaaattgca
     3901 gaagccctgg acaaaagcat tttcaagata gaagaagcca ttctgaaagc actggaaaca
     3961 acgcctcccg aactggccgg cgatatctac cggcgcggtt tgtacctgac cggcgggggc
     4021 gcattactga ggggattgga taaaagacta agccagaaaa tcaagctccc ggtccatatt
     4081 gcggatgatc cgttaaaaag tgtggtcagg ggaacgggaa tctccctaaa aaactacgac
     4141 aggtatcctt ttgtcatgcg gtagttctga tcatgctgtt ttattattat aaatcctaac
     4201 acctgtgcgc aatgtttttc tcttcatcag aaagtacgcc aacttcctgt ttttcctggt
     4261 gttgcagatc atagcactct cctttctatt ccggtacaac aaataccacg aggccatatt
     4321 catgaatgct gcgggagaaa tcaccggcag cctggaccag cgctacaata ccgttgaata
     4381 ctattttaag cttaagaaga tcaatgaaca gctggctgca gagaatgtgc gcctgaacca
     4441 ggcactgaag gagaattacg agcggcccga agccggcgcc aaaatcgttt tggatacggt
     4501 gcagatcgat tccctccggc agatccaaaa atacagctgg cttggggcgc gcgtggtcgg
     4561 cagcacggtg agcacccaga ccaattttat caccattcac cggggctcca accagggcgt
     4621 tttgccgaat atgggagtgg tcagcccgct gggtatcgta ggcaccgtag tgaatgtgag
     4681 tgaaaatttt gccagcgtaa tgaccctgct ccaccgccag tacaaggtag tggttaagat
     4741 gaaaaacagc ggcgaccggg gtacggttga atgggatggg atcagtccca gctacattac
     4801 attaaaagac atacccaaaa gcgcgaaggt tcaaaaagga gattccgtgg tcaccagtcc
     4861 gacttcttcc ttattccccg gaaatatcat ggtggggatc gtagaagaga ttgcggagga
     4921 aaagagcagt aacttttata cgttaaagct gagacccgct accaatttct ttaatattga
     4981 atacgtgtat gtgatctcca attcgcagct tgttgagcaa aagcgaatgg aagattccac
     5041 ccacaaaaaa ttccaatgag cgaactgctg aaaaatatca tccggttcat cctgttcatt
     5101 tttgtacagg tattcatcct gttcaagatg cctcccctgc accgcttcat ctctccctat
     5161 ttatattatc tctatatttt atggctgccc ttcagcatgt cccgggccgg actcatgagt
     5221 gtggccttcc tcttcggttt aaccctggac tatttcatgc atacgcccgg cctgcatgcc
     5281 gctgcctgcg tgctgatcgc ctacctgcgg ccgtttgtgg tgaatatgct gatctcccag
     5341 gaaggagccg ataagaatta tctgtcgccc tccattacca gcatgggctg ggctccttat
     5401 ggaacctacg tgtttatact tacgctgttt catcacgggt atctggtttt tctggaatgg
     5461 ctgagtttcg gatctttttt gtattttata gggaaagtaa tcgcaacaac cgggatcagc
     5521 atgttgttga tattgcttac agaactatta ttcttccgga aagagaaatt caggacgaat
     5581 accgtataat caataatccc atatccggag atgcctgttt ttaatcaatc gcgcagcaat
     5641 atcattcgcc tcatctttgc ggggatgttc ctgatcatat ttgcccagct cttcaatctt
     5701 caggtcattt cggataaata taaaaagatg gcgcaggaaa atgcgctttt ccccaaacga
     5761 atttatccta cccggggtat cattttcgat cgccacaacc gggccatcct caacaatacg
     5821 atcatgtacg acctgatggt aaccccatcc caggttaaaa atatggatac ggcctatttc
     5881 tgccagctga tgggaatcga ttcggcagaa ttccggtccc gcgtgctcga tgcccggatc
     5941 cgcaacggcc ctttccggcc aaccattttc gaagatcttt tgccgcccga tctctatgcc
     6001 aaactggaag aaaatctctg gcgatttccc gggttcaacc tgcaggaacg cccggtgcgc
     6061 gtttatcctt ttgacgcggg tgcccatatc atgggctatg tgggggaagt ggactcgaat
     6121 attttaagac gctccaaatt ctattatcag ctgggagatt atgtaggcag aagcgggctg
     6181 gagcagtttt atgaaaaaca gctcatgggt cagcgcggga tcgagtactg gatcaaggat
     6241 aataaaaacc gcctggtcgg ccattatgcg aaccg
//
LOCUS       DASYCU010000201         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1495826, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000201 DASYCU010000000
VERSION     DASYCU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495826"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VGM24_04055"
     CDS             <1..923
                     /locus_tag="VGM24_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEY4154568.1"
                     /translation="GLVCVSAVADYEKDIIKKHELTRPEKELDRINHMKITGAQTGNV
                     FLAHKSNTHLSGLIRDWKKSHQPVYDFIAEDGVGHMIWVVDDKATIQRISDIFNDEIP
                     FTYIADGHHRAASASKVKNALGKNSGPEADFFLTTLFPADELRILDYNRLVKDLNGLK
                     TAEFLEKLNQDFTVEISDTAFSPAQLHSFGMYLENHWYRLEAKEGSYRKDPIGVLDVS
                     ILQENILSPLLGIADPRTDTRVDFVGGIRGLAALEERVNSGNMKVAFSLYPVSMDQLF
                     SIADSGQIMPPKSTWFEPKLRDGLLTHLIR"
     gene            997..2010
                     /locus_tag="VGM24_04060"
     CDS             997..2010
                     /locus_tag="VGM24_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4154569.1"
                     /translation="MKKVLFFTGLFLLGLSAFSQSADSLHKMARTYMWSGDYPNAIIV
                     LSNALKQDPGNLDIEKDLAFTYYLQKNYSASVEAGRKLINRKDADEQCYQILGMAYKA
                     IEEKKEADKMYRQGIKKFPNSGVLYNEYGEILWTKQDPEAIRLWEKGIEVDPSYPSNY
                     YNACKYYYLTYDKIWTLIYGEIFINLESYSRRTPEIKSLLVDGYKKLFIDLKAPKSVN
                     DKNPFVAACLSVMNDQSATVSLGISPEALGILRSKFILEWFDKYAGRFPFHLFDYERQ
                     LMKAGLFDAYNQWIFGASMGLASFQNWTAQHTDEYNRFINFQKGRVFKLPPGQNYRAV
                     TAK"
     gene            2017..>3392
                     /locus_tag="VGM24_04065"
     CDS             2017..>3392
                     /locus_tag="VGM24_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4154570.1"
                     /translation="MTKPTRLFDCIAWQLEKFPKEDMLAAKENGVWRKYSSMEIEQQV
                     KRLAAGLLSLGISGNDLSLEGQDKIGIISNNRAEWLITDLAVQKLGAVLVPVYPTTHP
                     QEIEFIFQDAGVKYVFVSSEELYQKIAAFRGRVPSIKNVYSFNQIPGADHWSSVLSLS
                     TPAGIEQVEGISKTIQADHLTTIIYTSGTTGTPKGVMLTHNNILSNVLSCKESFPFED
                     RPEYKALSFLPLNHIFEKMVSYIYLFSGNSIYYAESMDSIGANLLEVKPDLFCTVPRL
                     LEKVYERIMAKGAALTGLKRKLFFWAVELGRKYDNNRDAGFWYNFQLSIANKLIFSKW
                     REALGNNLKFIVTGGAACQERLLRIFSAAGIPIYEGYGPTENSPVISVNRKGKGLCKL
                     GTVGPVINGQEVRLNPDGEICVKGPSVMKGYFRRPDLTAETLIDGWLHTGDIGRFEDG
                     IFLRITDR"
BASE COUNT          959 a          839 c          822 g          772 t
ORIGIN      
        1 ccggcctggt ttgcgttagt gcggttgcgg attatgaaaa ggatattatc aaaaaacatg
       61 agctgacccg tcctgaaaaa gaactggacc gcatcaatca catgaagatt accggtgcgc
      121 aaaccggaaa cgtttttctg gcccataaat ccaataccca cctgagcggg cttatcaggg
      181 actggaaaaa atcccatcag ccggtatatg attttatcgc cgaagacggg gtcggtcata
      241 tgatctgggt ggtggacgat aaagcaacca ttcagcggat cagtgatatt tttaatgatg
      301 aaatcccgtt tacctatatc gccgacggcc atcaccgcgc cgcttccgcc tcaaaagtaa
      361 aaaatgcact gggaaagaac agcggccccg aagcagactt tttcctgacc accctcttcc
      421 cggcagatga actccggatc ctcgattata accgcctggt gaaggacctg aacggcttaa
      481 agaccgctga attcctggaa aaacttaacc aggatttcac cgttgaaatc tccgataccg
      541 ctttttcccc ggctcagctg catagttttg gaatgtacct ggaaaaccat tggtaccgcc
      601 tggaagcaaa agaaggcagc taccggaaag atccgatagg agtactggat gttagcatcc
      661 tgcaggaaaa tatcctgagc cccctgctgg gcatcgcaga cccgagaacc gacacccggg
      721 ttgactttgt gggaggaata cgggggcttg ccgcgctgga agaaagagtg aacagcggga
      781 atatgaaagt cgctttcagt ctctatccgg tcagtatgga ccagctcttt agcatcgcgg
      841 acagcgggca gatcatgccc cccaagagca cctggttcga acccaaacta cgggatggcc
      901 tcttaacaca cctgatccgg taagcattcg tatttttgaa gaacaattat tcaacccgaa
      961 agccgttttt ttaagcgttc ggcctatatt cattgcatga aaaaagtcct gttttttacc
     1021 ggcctctttt tactcggtct ttcagccttc tcccaatcgg cagattccct gcataaaatg
     1081 gccagaacct atatgtggag cggcgattat cccaatgcca ttatcgtatt aagcaatgcc
     1141 ttaaaacagg atccgggcaa cctggatatc gaaaaagacc tggctttcac ctattacctg
     1201 caaaaaaatt acagcgcttc cgtggaagcc ggcaggaaac tgattaaccg gaaagacgcg
     1261 gatgaacagt gctaccagat cctgggcatg gcgtataaag ccattgaaga aaaaaaagaa
     1321 gcggacaaaa tgtatcgtca gggaattaaa aaattcccga acagcggagt gctttacaat
     1381 gaatacgggg aaatactctg gacaaaacag gaccccgaag ccatccgctt atgggaaaaa
     1441 ggaatcgaag tggatccttc ctacccctcc aactattaca atgcctgcaa gtattattat
     1501 ttaacctacg ataagatctg gaccctgatc tatggagaga tcttcatcaa cctggaaagc
     1561 tacagccgcc gcacacccga aataaaaagc ctgctggtgg atggctataa aaaattattc
     1621 atcgacctga aagcaccgaa atccgttaac gataaaaatc cttttgtcgc agcctgcctg
     1681 agcgttatga atgatcagtc ggccacggtt tccctgggca tttctccgga agcccttggc
     1741 atcctgcgca gtaaattcat tttggaatgg ttcgacaaat atgcaggcag attccccttc
     1801 catctttttg actacgaacg gcagttgatg aaggcaggcc tttttgatgc ctacaatcaa
     1861 tggatatttg gggcatccat gggactcgcc agttttcaaa actggaccgc gcagcataca
     1921 gacgagtata accgtttcat taattttcaa aaagggcggg tattcaagct accccctggc
     1981 cagaactacc gtgccgttac tgctaaatga tcccccatga ctaaacccac acgacttttt
     2041 gattgcattg cctggcaact ggaaaaattt cccaaggagg atatgctggc cgccaaggag
     2101 aacggggtct ggcggaaata cagcagcatg gaaattgagc aacaggtcaa gcgccttgca
     2161 gccggactct tatccctggg catatccggc aatgatctga gccttgaagg gcaggacaag
     2221 atcgggatca tttccaataa ccgggcggag tggctgatca ccgatctggc cgtacaaaaa
     2281 ctgggcgcgg tgctggttcc cgtttatccg accacccatc cgcaggaaat tgaattcatt
     2341 tttcaggacg ccggagtaaa atatgtattt gtaagcagtg aggaactata ccagaaaatc
     2401 gccgcattcc gcggaagggt tccctccatt aagaatgtat acagctttaa ccagataccc
     2461 ggtgcggatc actggtcgtc tgtactgtcc ctgtcaaccc ccgccggaat agaacaggtg
     2521 gaaggcatca gcaagaccat tcaggccgat catctgacca ccatcatata tacttccggc
     2581 accacgggca cgcctaaagg cgttatgctg acccataaca atatcctgag taatgtcctt
     2641 tcctgcaaag aaagtttccc gttcgaagac cggccggaat acaaggcact cagttttctt
     2701 ccgctgaatc acatctttga aaaaatggtc agttatatct atctcttcag cggcaacagc
     2761 atttattacg cggagagtat ggacagcatc ggcgccaacc tgctggaagt aaaaccggac
     2821 ctgttttgta ccgtgccgcg tttgcttgaa aaagtgtatg aacggatcat ggctaaagga
     2881 gctgcgctca ccgggctaaa aagaaaatta tttttctggg ccgtggaact gggcaggaaa
     2941 tatgacaata accgggatgc cggcttctgg tataattttc aattatccat tgccaacaaa
     3001 ttgatattta gcaaatggcg ggaagcgctg gggaataatc tcaaattcat tgtaaccggt
     3061 ggcgcggcct gccaggaaag gcttctccga attttcagcg ctgcgggcat tccgatatat
     3121 gagggctacg gaccaaccga aaactcgccg gtcatttcgg tcaaccggaa aggaaaggga
     3181 ctgtgcaaac tgggaacggt aggcccggta ataaacgggc aggaagtgcg gcttaacccg
     3241 gacggagaaa tatgcgtaaa aggacccagc gtgatgaaag gatatttcag aagacccgac
     3301 ctcacggcgg aaaccctgat tgacggctgg ctgcatacgg gcgacatcgg ccggttcgaa
     3361 gatgggatat ttctccggat cacagaccgg aa
//
LOCUS       DASYCU010000202         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3091208, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000202 DASYCU010000000
VERSION     DASYCU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3091208"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VGM24_04070"
     CDS             complement(<1..1319)
                     /locus_tag="VGM24_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY4154571.1"
                     /translation="MRHLAWAFILIGLFVTSCKKVTPVVGGREDSLSNPENPPPHSHS
                     SNNLADSNSVGSAEGLTNPLAAYFVRQQRQSLIIPSNQSLQVGASDLTICAWVKFNTI
                     GRFNQVIAAKDNDSTVEGSDYWMGYNYVTQTLQFQVETRVVTRPAMAHTFGLPLTGQW
                     LFVVGWYDHNLGTVHIQINNGTVDSAIAEKETRVTNTAFSIGSDVAAGGFLDGAINSV
                     GLWKRVLTSEERATLFNLAFAAPAAPVGSGRNPGMIVNSARHSKDVPETLSGNIYSLE
                     FNGVSEYLNLRRPVTLNPKGSALSVWFKTDSLSQLQVIAGRSTSASTYISILNSSTIR
                     VQTDVAATYKDFTVDTLKSQTWYYLVFNRDAEGNSHVYLNGRESFSGAQPQPNYAISI
                     DQLGRYWDGSKGDWYSGNLADLRLYKQSLSADDIKSISTGTEPVTNPA"
     gene            complement(1526..2635)
                     /locus_tag="VGM24_04075"
     CDS             complement(1526..2635)
                     /locus_tag="VGM24_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY4154572.1"
                     /translation="MSEKLRKDAVPVTAARQDESLQYLKGRGAQLNTRNRFLRDEVSK
                     EHVEGIDDWSETNVATQYIEQESKTIVNKVDSPDVGMMYSMNPYAGCEHGCIYCYARN
                     VHEYWGYSAGLDFERKIIVKKNAAKLLRKFLMNPKWECEPLSMSGNTDCYQPAEKIYR
                     LTRGMLEVCNEFNQPVGMITKNAGMLRDKDLLQEMAKKNLVSILVSITSFNEDLRQKM
                     EPRTTTAGQRLRTIRELSELGVRAGVMLGPMIPGLNEHEMQRIMKEASDNGASFSAYT
                     FIRLNGAIKLLFHDWLYKNFPDRADKVWHLIEDAHDGKVNDSRWGVRMRGEGSIAELV
                     SQQYKKYGKLYGLNAGRWELDCTRFRRPGEQGKLF"
     gene            complement(2665..3276)
                     /locus_tag="VGM24_04080"
     CDS             complement(2665..3276)
                     /locus_tag="VGM24_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY4154573.1"
                     /translation="METLVQKMPGYRLNEYMLVLTPHEELRHRILKVKKEFSEKYSCV
                     QALWGKPHIMLARFTQYEMMEERVMNRLKAVAMGFRPFKVELKDFGTYPSHTIFINVV
                     SREPIRELVREIRESQRLLKSDNEHKPYFVDDPQITLAGKLLAWQYEKGWQEYAHRHF
                     SGRFMSDGMLILKRRQGEKAWQIVERLPFQNLPVSTRQGELFG"
BASE COUNT          850 a          935 c          791 g          973 t
ORIGIN      
        1 gcagggttgg tcaccggctc cgttccggtt gaaatggatt taatgtcgtc ggctgacaag
       61 gattgtttat acaatctcaa atcggccagg ttccccgaat accaatcgcc tttgctacca
      121 tcccaatatc tgcccagctg gtcaatgctg atcgcatagt ttggctgagg ctgggcgccg
      181 gaaaaggatt ccctgccatt aagataaaca tgcgagtttc cctccgcatc ccggttaaaa
      241 accagatagt accaggtttg gcttttcaat gtatcaacag taaaatcttt gtaggtggct
      301 gctacatctg tttgaactct tatcgtggag gagttcagaa tagaaatata cgtgcttgcg
      361 ctggtcgatc tgcccgcgat cacctgaagt tgggaaaggg aatccgtttt aaaccatacg
      421 cttaacgcgc tgcccttcgg gttcaacgtg accggccgcc tgaggttaag atattctgaa
      481 actccattaa attcaagaga atagatattc cccgataagg tttccggaac gtctttggaa
      541 tgccttgccg aatttacaat catgccggga ttgcgtccgc ttccaaccgg tgcagcaggg
      601 gccgcaaaag ccaggttaaa taaagtcgcc cgctcttcgg aagtcaatac tctcttccac
      661 aatccgaccg agttgatcgc cccgtctaaa aaaccgccgg cagccacatc tgaaccgata
      721 gaaaatgcgg tgttcgtcac ccgggtctcc ttttcagcaa tggcggaatc cacggtcccg
      781 ttgttgatct ggatatgcac tgttccgaga ttatgatcat accagccaac cacaaaaagc
      841 cactgcccgg taagcggcaa accaaatgta tgggccatgg caggccgggt gaccaccctg
      901 gtttccacct gaaactggag agtctgtgtt acataattat agcccatcca ataatcggaa
      961 ccctctacgg tggaatcatt gtcttttgca gctattacct ggttaaaccg gcctatggta
     1021 ttaaacttta cccaggcaca aatcgtaagg tctgaggccc cgacctgaag gctttgattt
     1081 gatggtatta ttaatgattg cctctgctgc cgtacaaaat aagcagccag ggggttggta
     1141 agtccttccg cactgcccac cgaattgcta tctgccagat tgtttgaact atgggagtgg
     1201 ggtgggggat tttccggatt gctgagggaa tcttcccggc ctccaacaac cggagtgacc
     1261 tttttacagg aagtaacaaa caacccaatt aatatgaatg cccaagccaa atgccgcata
     1321 ctactattcc gtttacctta ttgtattctg tttaccttat tgcccggctt aaaaaaaatc
     1381 aaaaactgta cctgattttt agggttttct cccaaacatc agccccaaaa ccaatttaga
     1441 aacgcaagat gagtgattct ttggtatttc gcaaatatcc acccttttca ttccagggca
     1501 tatggaagac ttgcaccaat aaggctcaga acaatttccc ctgttctccc ggccttcgga
     1561 accgcgtgca atccaactcc catctaccgg cattcagtcc gtagagtttg ccgtattttt
     1621 tatattgctg gctaaccaat tctgcgatgg atccttcgcc gcgcatccgg actccccagc
     1681 gggaatcgtt cacttttcca tcatgggcat cttcaatcag gtgccagact ttatcggccc
     1741 ggtccgggaa attcttataa agccagtcgt ggaacaataa tttaatggcg ccgttcaggc
     1801 ggataaaagt atatgcagaa aagctggctc cgttgtcgct ggcctctttc atgatccgct
     1861 gcatttcgtg ctcattcaac ccgggaatca ttggtccgag catgacccct gcacgcacgc
     1921 ccagttcact cagttcccgg atcgttctta aacgctggcc tgccgtggtc gtgcggggtt
     1981 ccattttctg ccgaaggtcc tcattgaagg aggtgatgga aaccagtatg gagaccaggt
     2041 ttttctttgc catttcctgc agcaggtcct tgtcgcgcaa catcccggca tttttcgtga
     2101 tcatacctac cggctggttg aattcattgc agacttccag catgccccgg gtcagccggt
     2161 aaattttttc tgccggctga taacaatcgg tattcccgct catgctgagc ggctcgcatt
     2221 cccatttcgg attcatcagg aatttccgca gcagcttggc ggcatttttc ttcacaatga
     2281 tcttgcgttc gaaatccagt ccggcactgt aaccccaata ttcatgcaca ttccgggcat
     2341 aacaataaat acagccgtgc tcacagccgg catagggatt catgctgtac atcatgccca
     2401 catccggact gtcgacctta ttcactatcg ttttcgattc ctgctcgatg tattgtgtgg
     2461 ccacatttgt ctcggaccaa tcgtcaatac cctccacatg ttctttgctg acctcatccc
     2521 gcaggaagcg gttcctggta ttaagctgtg ctccgcggcc tttgagatac tgcaaggact
     2581 catcctgccg ggcggcggtg accggcaccg cgtccttcct tagtttttca gacattccat
     2641 tattttgatg aggaacaggc tttatcagcc aaataattct ccctggcggg tcgataccgg
     2701 aagattctga aacggcaacc gttcaacgat ctgccaggcc ttttctccct gccttctttt
     2761 taaaatcaac atcccgtcgc tcataaaccg gccactgaaa tggcggtggg catattcctg
     2821 ccagcccttc tcatattgcc aggccaggag ttttccggct agcgttatct gcggatcatc
     2881 cacaaaatag ggcttatgtt cgttatcgga tttgagcagg cgttgtgact cgcgaatctc
     2941 ccggacaagt tcccggatgg gttcccggga aacgacattt atgaagatgg tgtgcgaagg
     3001 ataggttccg aaatccttca gttccacctt gaaaggtctg aagcccatgg ccaccgcttt
     3061 caggcggttc atcactcttt cttccatcat ctcgtactgg gtgaatcgcg ccagcatgat
     3121 gtggggcttg ccccagagcg cctgaacaca gctgtatttt tcactgaact ctttttttac
     3181 tttaagaatc cggtgccgca attcttcgtg cggcgtcagg accagcatgt attcgttaag
     3241 cctgtatccc ggcatctttt gtactaaagt ttccataatc tggtgttttt ggctgccttt
     3301 gaataatgga aacaattccc gtgccccggc cgtttacctg ccacgggaca ctgttaaatt
     3361 tccatgatat tccgggagac cgtcagcccg tttactaaat ttattggtat aaatatacta
     3421 ataattttag taatcaaata aaaaaatttc ctctcccggc cggatacaaa aacatatccc
     3481 cgcatcaata actatcttgg gcggaattaa gatttgctta acaggaaatg cattcatgca
     3541 aagaataaa
//
LOCUS       DASYCU010000203         7723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5121760, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000203 DASYCU010000000
VERSION     DASYCU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5121760"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..611
                     /locus_tag="VGM24_04085"
     CDS             180..611
                     /locus_tag="VGM24_04085"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4154574.1"
                     /translation="MADPEIDLIPYLSFDGNCEEALNFYKGILNGRIEVMNRYNNPAM
                     NAPEEYKNKILHARFYFGNQTLFASDVMPGKSGNPLPSGNIALSLGLTDDVLAEKIFD
                     QLAAGGKIGIPFKKQFWGDWHGNLVDQYGIRWMVNAANAGK"
     gene            833..1402
                     /locus_tag="VGM24_04090"
     CDS             833..1402
                     /locus_tag="VGM24_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY4154575.1"
                     /translation="METKSAKKTNRTNSNSSAGSAANDSRLKEFFTDELKDILWAEKH
                     IVQTLPKMKKAANSEELKNAFDEHLEVTKTHVTRLEKAFGMLGEKATAKKCDAMEGII
                     KEGASIIEDTEDGTATRDVGLVLAAQKVEHYEIATYGGLIQLAKTLGHEDVADLLYET
                     IQEEEEADQLLTKIAENHINYEAAQEAEH"
     gene            1498..3045
                     /locus_tag="VGM24_04095"
     CDS             1498..3045
                     /locus_tag="VGM24_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4154576.1"
                     /translation="MKKVTAPLLLAGLFLAGTIYGQQATVLLHPDEAKYTISKNIYGH
                     FAEHLGHCIYDGIYVGDSNTVIPNTDGVRNDIIAALKKLKVPVLRWPGGCFADTYHWK
                     DGVGPKKDRPSMVNRWWGGVTEDNSFGTHDFLNLCERIGADPYLAGNVGSGTVQELSQ
                     WVQYVNFEGKSPMSDWRRANGRDKPWRVKYFGVGNEAWGCGGNMTPEYYANIYRQYAT
                     FMTDWQNGDHLFRVASGANSGDFHWTEVLMRDIPHNLLEGVALHYYSSLDWNNKGSAT
                     DYTEQQYFGTLKSALRMEELVTKHSAIMDKYDPKKKIALVVDEWGGWYNVEPGTNPGF
                     LYQQNTMRDAMIAGVTLNIFNNHADRVRMANLAQMVNVLQAVILTNKEKIILTPTYHV
                     MEMYNVHQDAELIPVTLANIPYTQGRDTLPAISASASRDKEGVTHLSLVNIDPDKTNI
                     ITVKFDGTGYKEVSGRILSSPKIQDHNSFDDPDKIKPVPFTGAAIKGSSLQVRLPPAS
                     VVILSLK"
     gene            3124..4476
                     /locus_tag="VGM24_04100"
     CDS             3124..4476
                     /locus_tag="VGM24_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4154577.1"
                     /translation="MKTRLIFFFCLIGGTGILSAPAQAQKILLRNVTLIDGSGSGAKQ
                     HMNVLLEGDRISGISKDNLKEPANVLVKDYTGKTIMPVLVCAHAHVGVLKGTKIEKEN
                     YTRENILRQLNRYQEYGVGAVLCMGTDRSLIFHGFRDSSQAGELPGARLYSAGHGFAV
                     PGSPPGGSWTDQLFRPGTPDQVPAEMDSLARLHPTVVKMWVDDFGGHAAKMKPEVYEA
                     IIREAHKHGLRVAAHLYYLEDARRLVDAGLDIIAHSIRDRLIDDDLLRKMKERKVAYI
                     PTLTRDEFTYIYADRPGWMDDAFFKASLEPGVYEMISSAAYRDKVRNSADYLQNKYAV
                     EIAMKNLKKIADAGILVALGTDSGADPVRAQGFSEQRELELMVEAGLSPLKAIQVGTE
                     NAARVLKINDRYGTLQPGKKADFIVLSASPEKEITNTRKIISVWKNGREVSRGPLSGR
                     "
     gene            4552..5529
                     /locus_tag="VGM24_04105"
     CDS             4552..5529
                     /locus_tag="VGM24_04105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY4154578.1"
                     /translation="MRVFITRIIPDAGLQLLKQAGIEFTQWGEKRDLTPRELIDHCQP
                     ADALLSAGGNRIDREFLNACRHLKVISLHSVGYDRVDVAAATELKIPVGNTPAVLSDA
                     TADIAFLLILATSRKAFYHHKSIIQGKWKFFDPTADLGIEIRGKTLGVFGLGKIGFEL
                     AKCCAAAFQMKVIYHNRHRNEEAEKKLNAEWVSFDDLLRRADVLSVNTALTSETKGRF
                     NKEVFAKMKPSSIFINAARGAIHNEPDLIDALQNGAIWGAGLDVTDPEPMHPDNPLLQ
                     MPNVSVLPHIGSATVETRNAMAVIAAKNVIAGLRGEELPFAVNPGVKKN"
     gene            complement(5542..6456)
                     /locus_tag="VGM24_04110"
     CDS             complement(5542..6456)
                     /locus_tag="VGM24_04110"
                     /inference="COORDINATES: protein motif:HMM:NF040196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HEY4154579.1"
                     /translation="MSIKNLACIAALLFSQLPGRAQNDSLLHLLNDSLASSGTSSYVT
                     GTFKATHVINMQTIEAPAAGALNVMIQHRFGQLNSGSYNFFGLDNASLRLGLDYGISN
                     RLAIGIGRSSNQKTFDGYLKYKLLRQTEGAHIIPVSISLLGTISNYTQHYTDKPFLNA
                     GYRTSYSTQLLIARKLSSHLSVQLTPTYLHFNLVPTVKDKNDVFAIAGGARLRITNRM
                     SINAEYNYLLPDQLVSLDLRNSFSLGWDIETGGHVFQLVFSNSQGMIESQYIGQTTGR
                     WSKGDIYFGFNISRNFNLGKKAKRNSGG"
     gene            complement(6463..7017)
                     /locus_tag="VGM24_04115"
     CDS             complement(6463..7017)
                     /locus_tag="VGM24_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY4154580.1"
                     /translation="MRPGKLVLCILSVLLICTGGKAQAPYMARTGFIGFYSKTPLEDI
                     IAENNQVFAVIDTAKKNLAFALLLKGFIFRKELMQEHFNENYVESDKYPKAEFSGTYT
                     CSRTMDKEGVYPIMVKGNLTLHGVTKSIEEPATLEIKSGSLLANSNFQLKPEDFRISI
                     PSIVRDKISKEMNVKVQVTWMTKK"
     gene            complement(7050..7433)
                     /locus_tag="VGM24_04120"
     CDS             complement(7050..7433)
                     /locus_tag="VGM24_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4154581.1"
                     /translation="MKIRLHLFLLLIGSSFFLVLSCSKTSEDKLNNNGGTCDTTNMQY
                     TADVVPILQANCYSCHGAGNTDGSGGISLDSYADLKKYADNGFLKGNITHAPGYVGMP
                     YGRPKLDDCDINKIIDWINRGTQND"
     gene            complement(7477..>7723)
                     /locus_tag="VGM24_04125"
     CDS             complement(7477..>7723)
                     /locus_tag="VGM24_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154582.1"
                     /translation="ANKKFLGKVIEVTGSISGIQKDTGNAGILMDATAMGGVNCGFSK
                     TALPGLQLLKTGDTIRIKGRCTGFLMDVNLVDCVLEK"
BASE COUNT         2055 a         1739 c         2055 g         1874 t
ORIGIN      
        1 tggatatccg gttgacccag gcggttttgg atcagctcga ttccctcctg cgtaaatatc
       61 ccgataccgg tgaacggtat agcgagggtt cgatgaaact ggtaaataaa taaatgcagc
      121 ggaaggaagg ggtggaataa gtatatttag aagcgcttat tcaataaaaa aattatttta
      181 tggcagatcc ggaaattgac ctcattcctt acctaagctt cgacgggaat tgcgaggaag
      241 cactgaattt ctataaagga attctgaatg gacgcatcga agtgatgaac cgctataata
      301 atccggctat gaatgcgccg gaggaataca aaaataaaat tctgcacgcg cggttttatt
      361 tcggcaacca aacccttttt gccagtgatg ttatgcccgg caaatccggg aacccgctgc
      421 cctcgggaaa tatcgccttg tcgctgggcc taaccgacga tgtactggcg gaaaagattt
      481 ttgatcagct ggcagccggc ggaaagatcg ggatcccatt taaaaaacaa ttctgggggg
      541 attggcacgg gaatctggtg gatcagtatg gcattcgctg gatggtcaac gcggcgaatg
      601 cagggaaata gctccggaat atcctaatgt tttcagaaaa aatctgtttt cttccttccc
      661 aaatattacc cacatcccgg tttgccgtgg aataatatgc ccggtttccg gcatggattt
      721 gccgggtgtt tccggatctc ttcttacgat ggatcgcttt cccgttcacc catttcctgg
      781 cacagatttt taacccattc tgttttacat tctaaaacta aaaagattga atatggaaac
      841 aaaatctgca aaaaaaacga acaggacaaa ttcaaattca tccgccggca gtgcggcaaa
      901 tgattccagg ctgaaagaat tttttaccga tgagctgaaa gacattctct gggcagagaa
      961 acacattgta cagaccctgc ctaaaatgaa aaaagcagca aactccgagg aattgaagaa
     1021 tgcattcgac gaacacctgg aagtaacaaa aacacacgtg acccggctgg aaaaggcctt
     1081 tggcatgctg ggagaaaaag ctaccgccaa aaagtgcgat gccatggaag gaatcattaa
     1141 ggaaggcgcc agtatcattg aagatacgga agatggaacc gccacccggg atgtcgggtt
     1201 ggttctggca gcccaaaaag tggaacatta cgaaatagcc acttacggcg gactgatcca
     1261 gctggcaaag accctgggtc acgaagacgt cgcagatctc ctttatgaaa ccatccagga
     1321 ggaagaagag gcggaccagc tactcacgaa gattgccgaa aatcatatta attatgaggc
     1381 ggcacaagag gcggaacatt gattttaatt tttttttgaa ggccccgcag atgcggggtt
     1441 ttttttatgc cctctccgtt tgacgggttc tttgtaagtt gtgcaaaata aataagcatg
     1501 aagaaagtta ccgcgccgct tttgctggcg ggattattct tagccggcac aatatacggc
     1561 cagcaggcta cggttctcct gcaccccgat gaggcaaaat atacgatcag caaaaatatc
     1621 tatggtcatt ttgccgagca cctgggtcat tgcatctacg atgggatata tgtcggcgat
     1681 agcaataccg tcattccaaa tacggatggg gtacgcaatg atattatcgc ggcattgaaa
     1741 aagcttaaag tgccggtgct tcgctggccc ggcggctgtt ttgccgatac ctaccattgg
     1801 aaggacggcg tgggtccgaa gaaggaccgg ccgtccatgg taaataggtg gtggggcggc
     1861 gttaccgaag acaatagttt cggtacgcat gattttttga atctgtgtga acgcatcggt
     1921 gcggatccct acctggctgg gaacgtgggc agcggcacgg tgcaggagtt atcccagtgg
     1981 gttcaatacg taaattttga gggcaagagc ccaatgtcag actggcgaag ggcgaacggc
     2041 cgcgacaaac cctggcgggt aaaatatttc ggagtgggta atgaggcctg gggttgcgga
     2101 gggaatatga ccccggaata ttacgccaat atctatcgtc aatatgccac gtttatgacc
     2161 gactggcaaa acggcgatca cctgttccgg gtggcttccg gggctaattc gggagatttt
     2221 cactggacgg aagttttgat gcgggatatc ccgcataatt tgctggaagg cgttgccttg
     2281 cattattatt catcgttgga ttggaataac aagggttctg ctacggatta cacggaacag
     2341 caatattttg gcaccctcaa atcggcgctc cggatggagg aactggttac caaacattcg
     2401 gcgatcatgg ataaatacga tccgaaaaaa aagatcgcgc tggtagtgga cgaatggggt
     2461 ggctggtata atgtggagcc cggcaccaat ccggggtttt tgtatcagca gaatacaatg
     2521 cgggatgcga tgattgccgg cgtgaccctg aatattttca ataaccatgc tgaccgggtg
     2581 cggatggcca acctggccca gatggtgaac gtactccagg cggtcattct gaccaataag
     2641 gaaaagatca tcctgacgcc tacctaccat gtgatggaga tgtacaatgt gcaccaggat
     2701 gcggaattga ttccggtaac gctggccaat attccttaca cccagggaag ggataccctg
     2761 ccggccatct cggcctctgc atccagggac aaagaaggcg ttacccatct ctcgctcgtg
     2821 aacattgatc cggataaaac caatatcata acggtgaaat ttgacgggac cggatataaa
     2881 gaggtcagcg gcagaattct ttcatcccct aaaatccagg atcataacag ttttgatgat
     2941 ccggataaaa taaagccggt ccctttcacg ggcgccgcaa taaaaggcag cagcctgcag
     3001 gtgaggctgc ccccggcttc cgtcgtcatc cttagcttga agtgaccggc cccatgaatt
     3061 tatttccaaa acagcgaact ttgtccggta atatagaagt ttgcacatca aatggataag
     3121 cggatgaaga ccaggctaat ttttttcttc tgtctgatcg gcggaacagg cattctttcc
     3181 gcacctgccc aggcgcagaa aatattatta aggaacgtga ccctcataga cgggagtgga
     3241 tcgggagcaa aacagcacat gaatgtcttg ctggaaggag atcggatttc cggaatatca
     3301 aaagataact tgaaagaacc agccaatgtg ctggtaaaag attataccgg caaaaccatc
     3361 atgccggtac tcgtctgtgc ccatgcgcat gttggcgtac ttaaagggac aaagattgaa
     3421 aaggaaaact atacccgcga aaatatcctg agacagctaa acagatacca ggaatatggg
     3481 gtgggggcgg tgctttgcat gggcaccgac cggtccctga tctttcacgg cttccgggat
     3541 tcatcgcagg ccggagagct gcctggtgcg cgactctatt cggcaggtca cggattcgcc
     3601 gtgcccggct caccgcccgg gggatcctgg acagatcagt tattccggcc gggaacgccg
     3661 gaccaggtgc cggccgaaat ggattccctg gccaggctgc atccgacggt tgtaaaaatg
     3721 tgggtggatg attttggcgg acatgcggca aagatgaaac cggaggttta tgaggccatt
     3781 atccgggaag cgcataaaca cggattacgg gtggcggcac atttatatta tctggaggat
     3841 gcgcgccggc tggtggatgc cggtttggac attattgcgc acagcatacg cgaccggttg
     3901 atcgatgatg accttttgag aaagatgaaa gaaaggaaag tggcctatat tccgacactt
     3961 acccgggatg aatttacgta tatctatgcg gaccggcccg gatggatgga cgatgcgttt
     4021 ttcaaagctt cactggaacc gggcgtatat gagatgatca gcagcgccgc ctaccgggat
     4081 aaggtcagaa attcggcgga ttatcttcag aacaaatatg ccgttgaaat cgcgatgaaa
     4141 aatctgaaga agattgcgga cgccggcatc ctggtggccc tgggcacgga ctccggtgcc
     4201 gaccccgtgc gtgcccaggg attttctgaa cagcgggaac tggagttaat ggtggaggcc
     4261 gggctgagcc cgctcaaagc gatccaggtg ggcacagaga atgcggccag ggttctaaaa
     4321 ataaatgacc gctatggtac gcttcaaccg ggaaagaaag cggattttat cgtgttgtct
     4381 gccagtccgg aaaaagaaat aacaaatacc aggaaaataa tatcggtctg gaagaatggc
     4441 cgcgaagtga gtcggggtcc tttatccggg aggtaaaacc ctgccgcttt attatttcct
     4501 cctggcaaga aaagccttcc tgcggatatg ttcaattaat aagcgaatga gatgagagta
     4561 ttcataacga gaataattcc ggatgccggt ttgcagttat taaagcaggc aggaattgaa
     4621 tttacccaat ggggagaaaa aagagattta acgccccggg aactgattga ccattgccag
     4681 cccgctgacg cattgctgag tgccggggga aaccggatag accgcgaatt tttaaatgcc
     4741 tgccggcatc tgaaagtgat cagcctgcat tccgtgggct atgacagggt ggatgtggct
     4801 gcggcaaccg aactgaaaat tccggtcggg aatacgccgg cggtgttaag cgacgcgacg
     4861 gccgatattg cgtttttgct gatcctggcc acctcgcgta aggcattcta ccaccataaa
     4921 tcaatcatcc agggtaaatg gaaattcttt gaccccacgg ccgatctggg tattgaaata
     4981 agaggcaaaa ccctgggggt attcggtttg ggtaaaatag gatttgaact ggcaaaatgc
     5041 tgtgcggcag catttcaaat gaaggtcatt taccataacc ggcaccggaa tgaggaggcg
     5101 gaaaagaagt tgaacgcgga atgggtttca ttcgatgacc tgcttagaag agccgatgta
     5161 ttgagcgtaa acacggcact gacctctgaa acaaaaggca gatttaataa agaggtcttt
     5221 gcaaagatga aaccttcatc cattttcatt aatgccgccc ggggagcgat ccataatgag
     5281 cccgacctga tcgatgcatt acaaaatggg gctatctggg gggcgggcct ggatgttacg
     5341 gatcccgagc ccatgcaccc ggacaatccc ctgctgcaaa tgccgaacgt atcggtgctg
     5401 ccgcatatcg gttctgcaac agtagaaaca cggaacgcca tggcggttat tgcggcgaaa
     5461 aatgtgatcg cgggattgag gggagaagaa ttaccatttg ccgtgaatcc gggagtgaaa
     5521 aaaaattgat ggtcccttct cttatccgcc actatttcgt ttggcctttt ttcccaaatt
     5581 aaagttccgc gaaatattaa agccaaaata gatatctccc ttgctccacc ggccggtggt
     5641 ctggccgata tactgtgatt ctatcatgcc ctgggaatta ctgaacacca gctgaaatac
     5701 atgaccgccg gtttcaatat cccagcccag ggaaaaagaa ttgcgcagat ccagggaaac
     5761 gagctgatcg ggtaaaagat aattatattc ggcattgatg ctcatccggt tggtaattct
     5821 caggcgggct ccgccagcaa tggcaaaaac gtcgttctta tcctttacgg tgggaaccag
     5881 gttgaaatgc agataggtcg gcgtcagctg gacagaaaga tggctgctca gtttccgggc
     5941 gatcagcaat tgcgtggaat aggaagtccg gtatcccgca ttcagaaagg gcttgtctgt
     6001 ataatgctgg gtataattgc tgatcgtccc cagcagactt atggaaaccg gaatgatgtg
     6061 tgcaccttcc gtttgccgca gtaatttata ttttaaatac ccgtcaaatg ttttttggtt
     6121 ggagctccgg ccgatgccga tggccagcct gttgctgatg ccataatcca ggccgagccg
     6181 gagagaggca ttgtccagcc cgaaaaaatt ataactgccg ctgttgagtt ggccgaaccg
     6241 gtgctggatc atcacattca atgcaccggc cgcgggggct tcaatcgttt gcatattgat
     6301 cacgtgggtg gctttaaacg ttccggtaac gtaactggag gtgcccgaag acgccaggga
     6361 atcattaagc aaatgaagca gggaatcatt ttgtgcgcgt cccgggagtt gggaaaaaag
     6421 gagggcggca atgcaggcta agtttttgat tgacatggtt gtttattttt tggtcatcca
     6481 ggttacctgg acctttacat tcatttcttt ggaaatcttg tctcttacta tggaaggaat
     6541 ggagatccgg aaatcttcgg gctttaattg aaagttgctg tttgccagga ggctgcccga
     6601 ttttatctcg agggtggcgg gctcttcaat ggatttggta accccatgaa gcgtgagatt
     6661 tcctttcacc atgatgggat aaacgccttc cttatccatt gtccggctgc aggtatacgt
     6721 gccgctgaat tcggcttttg ggtatttatc gctttctaca tagttttcat tgaaatgctc
     6781 ctgcatcagt tctttccgga aaatgaaccc ctttaacaat aaagcgaagg ccaggttttt
     6841 ttttgccgta tcaatgacgg caaacacctg gttattctct gcgatgatat cttccaaagg
     6901 ggtcttggaa taaaaaccga taaagccggt ccgcgccata tagggtgcct gcgctttacc
     6961 gccggtgcag atcagcagca cgctgagaat acataagaca agttttccgg gcctcataga
     7021 aattttataa taatgagtga atgattttcc tagtcgtttt gcgtaccccg attgatccag
     7081 tcgataattt tattgatgtc gcaatcatct aatttgggcc ggccataggg catgcccaca
     7141 taacccgggg catgggtaat attccctttc agaaagccgt tatcggcata ttttttaaga
     7201 tcggcatagc tgtccaggct gattccgcca ctgccatcgg tattgcccgc tccatgacag
     7261 ctatagcagt tcgcctgcag gatgggaacc acgtcggcgg tatactgcat gttggtggta
     7321 tcacaggtgc ctccgttgtt gttgagcttg tcctcgctgg ttttcgaaca ggaaaggacc
     7381 aggaagaagg aagagccgat taagagcaaa aagagatgga gccggatttt cataatgatc
     7441 aattaatgct tgaatgaacg attgtatatt ctatgcttat ttctccagga cgcagtccac
     7501 gaggttgaca tccatcaaaa agccggtaca ccggcctttg atgcgaatgg tatctcctgt
     7561 tttcagtaac tgcagccccg gaagcgcggt ttttgagaag ccgcagttta cgccccccat
     7621 ggcagtcgca tccatcagga tgccggcgtt tccggtatcc ttctgtattc cggaaatgct
     7681 gccggttact tcaatcacct tgcccaggaa ttttttattg gcg
//
LOCUS       DASYCU010000204         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3961795, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000204 DASYCU010000000
VERSION     DASYCU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3961795"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGM24_04130"
     CDS             <1..194
                     /locus_tag="VGM24_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HEY4154583.1"
                     /translation="TGFHAISGISSFPAGADYYIDMLGIDFHYRSMSRGLITIRDLIY
                     FGTVILFFLTLTRNNLNKR"
     gene            202..>1535
                     /gene="gldG"
                     /locus_tag="VGM24_04135"
     CDS             202..>1535
                     /gene="gldG"
                     /locus_tag="VGM24_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HEY4154584.1"
                     /translation="MHKIFSSKSGWFFLLIGLILVNILASYWPLRFDLSSEKRYSLSA
                     PVKKMLGNIHDEVRIDIYLQGNLKQGLRKLRNSTEALLQEFNDYADGNIHYRFVDPVA
                     GGDDSTRAMILDSLRRMGIQPMTQVAQVKKGEEQTQRIVIPAAMLRYKDRVFPVDLLK
                     GVQRSREGQAPEQLYVNAETLLEYKFANAIDKLVKDTLPAVGYLMGNGEPLDFRVYSL
                     IQYLRENFRFGMVRLDSLPVIPNRLNALILVKPSVKFSDDEKLKLDQFVMRGGSLICM
                     IDNLHAESDSLQMSKEVIAYDRGLNLDDLLFHYGVRINQDLVEDMQNASINLVVGSQG
                     GKPQFQLLPWPYYPLLDGNPSNSISKNLDPVFSRFANSIDTVKADGIQKSFLLQSSPN
                     ARTISTPAMISLESIKTANDPKEFNRQHIPVAVLLEGKFNSLYANRISPAME"
BASE COUNT          390 a          364 c          387 g          394 t
ORIGIN      
        1 atacaggctt tcacgccatc agcggcattt cttccttccc ggccggagca gattattaca
       61 tagatatgct gggcattgat tttcattatc ggagtatgag ccgcggactg atcaccatac
      121 gcgacctgat ttatttcggg actgtgatcc tgtttttttt aaccctcacc agaaataacc
      181 tgaataaaag atagcctgac catgcataag atcttctcat ccaaatcagg ctggttcttt
      241 ttgttgatcg ggttgatcct ggttaatatc ctggcttctt actggcccct gcgttttgac
      301 ctcagcagtg aaaaaagata cagcctgagc gcgccggtaa aaaaaatgct gggcaatatt
      361 cacgatgaag tgcgcatcga tatttatctg cagggaaatt taaaacaggg tctgcgcaaa
      421 ctcagaaata gtaccgaagc tttattgcag gagttcaacg actatgcgga cggaaatatt
      481 cactaccggt ttgtggatcc ggtggcaggc ggcgacgatt ccacccgcgc aatgatcctg
      541 gattcgctcc gccggatggg cattcaaccc atgacccagg ttgcccaggt gaaaaagggc
      601 gaagaacaga cccagcggat tgtgatcccg gcggccatgc tgagatataa ggaccgggtg
      661 tttccggtag acctgcttaa aggagtgcag cgcagccgcg aaggccaggc tcccgagcaa
      721 ctttatgtaa atgccgaaac cctgctggaa tacaaatttg ccaatgcgat tgataaactg
      781 gtgaaagaca cgctccctgc cgtgggttat ctgatgggca atggagagcc gcttgatttc
      841 cgggtgtata gcctgataca gtatctgcgg gaaaatttcc ggtttggcat ggttcgcctg
      901 gacagtctcc cggtgatccc gaaccgcctg aatgcgctca tcctggttaa gccttctgtt
      961 aaattttctg atgatgaaaa gcttaagctc gatcagttcg tgatgcgggg cgggagcctg
     1021 atttgcatga tcgacaactt acatgcggaa tccgatagtt tgcagatgag taaagaagtg
     1081 attgcttatg accgggggtt aaacctcgac gatcttttat ttcattatgg tgttcgcatc
     1141 aaccaggatc tggtggagga tatgcagaat gcgagcatta atctcgtcgt gggcagccag
     1201 ggcggaaaac cgcagtttca gctgcttccc tggccttact atccgttgct cgatggaaac
     1261 ccttcgaatt ccatttcgaa aaatctggat ccggtttttt cccggtttgc caattccata
     1321 gatacggtta aagcagatgg tattcaaaag tcctttctcc tgcaaagttc accgaacgca
     1381 agaaccattt ccacacctgc gatgataagc ctggagagca tcaagacggc aaatgatcca
     1441 aaggaattta accggcagca tattcccgtg gctgtgttgc tggaagggaa attcaattcc
     1501 ttatacgcca accggatttc gcccgccatg gagga
//
LOCUS       DASYCU010000205         5723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_192325, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000205 DASYCU010000000
VERSION     DASYCU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192325"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VGM24_04140"
     CDS             complement(<1..1500)
                     /locus_tag="VGM24_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154585.1"
                     /translation="MFKNYLKIAWRNLRKDRQFTLLNLVGLSTGMACTLLIYLWVTDE
                     LNVDRFHQNDARLFQIMVNNHNAGTVSTVPQTPALLAQALAKEIPGIDYAAATVTTIG
                     LKKTTLSAGDKLTKAIGQYAGKDYFRMFSWHLLAGKEDQVLASKNSIVLSRDLAIRLF
                     GTAEKVIGKPVSWQKDKLYIVSGIFEGTPANSSIQFDYVMSYENFLDERPYEKDWNNS
                     DPNTYLVLKEGVDPARFNQRIAGFLKTKIPGTTASLYLQPFSSGYLHGKYENGVQAGG
                     RIEYVRLFSLIAVFILILACINFMNLSTAKAAGRMKEVGIKKVVGASRGALVLQYLSE
                     SMLMSFLSLLIALSLVAVLLPSFNALTGKQLALRFDSPLLLCIISVGLLTGLMAGSYP
                     ALYISGFAPVAILQRKLRNTPGELWIRKGLVVFQFTLSVSFIVSVLVVYRQIEFIQTA
                     NEGFNKENTISFDMEGKFSTPEEMKAFTTGVQSLLQEVKEVPGIVNASSM"
     gene            complement(1654..2034)
                     /locus_tag="VGM24_04145"
     CDS             complement(1654..2034)
                     /locus_tag="VGM24_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4154586.1"
                     /translation="MKNTNNKKMTTREIATRFNELAREEKWFEIQDEFFADNVKSIDP
                     PGSPYFGNAEGKDAVRKKGQDFVNRITAFHGASTSESIVAGNHFAVGREMDVTVTGHG
                     RIQVKEIMVYEVKDGEIISEQFIY"
     gene            2216..3490
                     /locus_tag="VGM24_04150"
     CDS             2216..3490
                     /locus_tag="VGM24_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154587.1"
                     /translation="MINTDDLHIEEEILPLFDLTFNTYSGEEVRDIITKIPGSKEQVL
                     FRQQLLKGFIANRELLKDYSYSRFDLTEIHNFLETFFIGSISGKSLRVKFMFSEKLRH
                     QKRGKLILLIRLFHKIHTSYLSRIDKKSFPPEYAVELQGLNEFFASFDLDRYETAFRK
                     NKFRIRHMVELMMLLTEKIMNGQMASFWKRWFSFEAWLSISESIIKHGYIFPSFDGTV
                     FSLEGFYHPVLKNPVKNDLTASHHVILLTGPNMSGKSTLLKAVSLCVYLAHAGLAVPA
                     SKATMPFFTGISVAINLTDSIVSGYSHFMSEVITLKKVLTKAASGGKNFAVFDELFRG
                     TNIEDALDISRATIRGIVHFPDTLFFISTHLHQLKELEEIKTHRVSTCFIECELQDNI
                     PVFTYKLKEGWSDLKLGRILFEREGLNTMLNI"
     gene            3534..>5723
                     /locus_tag="VGM24_04155"
     CDS             3534..>5723
                     /locus_tag="VGM24_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154588.1"
                     /translation="MLKRCIRWSCLIGTGFFLICQSLFIPDAALGKEPLPPQISIVTD
                     SLAGGCARQGLKKLTEILATRNISFEEVGSPEQAKGRMILVTGLAYDGSIASGLLRAG
                     QHTVDRAPEALTVWKTQWVNKKVIVISGYDETGLMYALTDVARQIRQNASSGDPLKEV
                     QEITEAPAIRDRAVSMYTMNRAYWESRFYDEKYWGQYLDMLSENRFNSLVIVFGYENG
                     GFLAPCYPYFFNVDEFPDVKMVGLSAEQQNKNLLAMNRLICMAHERGIRVTAGIWDHI
                     YRGGVQGGGMPGTEKAPDEPVAGLVWGLSANNLTAYTKAALEKFIREIPVDGIQFRLH
                     GESGLRKEEEASFWDDVFRMIKTVKPDLLVDLRAKELPPTAIPSAESAGIKFRIDTKF
                     WMEQMGLPYHPTRINPDPSYLRHSYGDLLRYPKKYDMNWRLWTGGTSRILLWGDPDYA
                     RRFMESVHLYDGSSYDVNEPLATKMEAQPEGIPPFDLLQPQHRYYTYEFERYWHFFQV
                     FGRLGYDPQASAAIWDKELALRFGKKTGPLVESALHKASAILPRIIASCYPYGAFPAT
                     RGWAEKQHLGDLPYYAKAEGSDLCQFANFDEEARLLLEGAGTAKIRPSANCQWFAKKA
                     KDIRALVLQIKGQSPDRRKELNSTITDLEILANLALYHSERIPAAVYYRLFVRTEDVA
                     ALDSAIAYEKKATEAWRKIVSSAGDYYDYNLRMGVSVAGLSGHWRDEL"
BASE COUNT         1474 a         1367 c         1433 g         1449 t
ORIGIN      
        1 catactggaa gcattgacaa tccccggcac ttcttttact tcctgcagca ggctttgcac
       61 tccggttgta aatgccttca tctcttccgg agtggagaat tttccctcca tgtcgaaact
      121 gatggtattc tccttattaa agccctcgtt ggccgtctgg atgaattcaa tctgccggta
      181 aacgaccagc acggatacga taaaggaaac agacagcgtg aattgaaaaa cgaccagtcc
      241 tttccggatc cagagttctc ccggcgtatt cctcagcttt ctctgcagga tagccaccgg
      301 tgcgaaaccg gaaatgtaaa gcgcagggta gctacctgcc ataagaccgg taaggaggcc
      361 cacgcttatt atacataaaa gcagggggct gtcgaatcga agggcaagct gctttccggt
      421 gagggcgttg aaggacggga gaaggactgc caccaggctg agggcaataa gcagcgataa
      481 aaagctcatc aacatggatt cgctcaggta ttgaaggacg agcgctcccc ggctggcgcc
      541 gactactttt tttatcccca cctctttcat tcttccggca gccttagccg tggaaagatt
      601 catgaaattg atacaggcga ggatcaggat gaacacggca atcagggaaa aaagcctgac
      661 atactctatg cggcctccgg cctgtacgcc gttttcgtat ttgccgtgaa ggtagcctga
      721 cgaaaaaggc tggaggtata aactggcggt ggtcccggga atttttgttt tcagaaaccc
      781 ggctattctt tgattaaacc gggccgggtc gactccttct tttaagacaa gataggtatt
      841 gggatcgctg ttgttccagt ctttctcata gggccgctcg tcgaggaaat tttcgtacga
      901 catcacatag tcgaactgaa tggaggaatt ggcgggtgtt ccttcgaaaa ttccggacac
      961 aatgtagagt ttatcctttt gccaggatac cggctttccg attaccttct cagcggttcc
     1021 gaataaccta atggccaggt cccgggaaag aacaatggaa tttttactgg ccagcacctg
     1081 gtcttctttt cctgcaagca ggtgccagga gaacatacga aaataatctt taccggcgta
     1141 ttgcccaatg gccttcgtta gcttatcgcc cgcggacagc gtggtttttt tcaacccgat
     1201 ggtcgtaacg gtagccgctg cgtagtcaat cccgggtatt tctttggcca gggcctgtgc
     1261 caggagcgcg ggtgtctggg ggaccgtgct gacggtgccg gcattgtggt tattgaccat
     1321 tatctgaaaa agccgcgcgt cgttttgatg aaaccggtcc acattcagtt catccgtcac
     1381 ccaaaggtag atgagcaggg tgcaggccat ccctgtcgaa agtccgacga ggttgagaag
     1441 cgtgaactgg cgatctttgc gcaggttgcg ccaggctatt ttcaaatagt ttttgaacat
     1501 aagcggaatt tattggcgaa agaggcacaa aaaggctacc agctttcaat ttgattgaaa
     1561 atccatattt tatggatttt gtacaaaagt tgagctgtcc ggaatcgata cagtgggtgt
     1621 gcggggttaa acggaacgta ttgcctctgc ccactaataa ataaattgct ccgaaataat
     1681 ctcaccatct ttcacttcat ataccatgat ttccttcacc tgaatccggc catgccctgt
     1741 aacggtaacg tccatttccc ttcccaccgc aaagtgattg ccagccacaa tcgactccga
     1801 ggtggaggcc ccatgaaagg ctgttatgcg attcacaaag tcctgcccct tctttcgtac
     1861 ggcgtccttg ccttcggcat tgccgaaata tggcgacccg ggaggatcaa tactttttac
     1921 attatctgca aaaaattcgt cctggatttc aaaccatttt tcttcccggg caagttcatt
     1981 gaaacgggta gcgatttccc gggttgtcat tttcttatta tttgtatttt tcattgttga
     2041 tctgttttta tttaaaatgt tcattaccca caaaaatacg atcgcctccg ggctattaaa
     2101 gtggctaaac cgacaattat gaggttgata atgccaaatc cggtaattta cgggttaacg
     2161 tctcctgaaa agcgtataga gacaagtacg gccgcggtgc acttaaaata agcaaatgat
     2221 caacacggac gatctgcaca tagaagagga gatcctgcca ttgtttgatc tcacttttaa
     2281 tacgtactcg ggcgaggaag ttcgcgatat tattacaaag atccctggct caaaagaaca
     2341 ggtactgttc cggcagcagc tgttaaaggg ttttattgcc aaccgggaat tgctaaaaga
     2401 ctattcttat tccaggttcg atttaactga aatacataat tttctggaaa cattttttat
     2461 cggatcaatt tcgggcaaaa gcctgagggt gaaattcatg ttttccgaaa agctgagaca
     2521 tcagaaaaga gggaagctca tattacttat ccgcctgttt cataaaattc ataccagcta
     2581 tctcagcagg atagacaaaa aatcatttcc cccggagtat gccgtcgagc tgcaggggtt
     2641 aaacgaattt tttgccagct tcgacctgga tcgttacgaa actgcatttc gtaaaaataa
     2701 attcaggatc cggcatatgg tggaactgat gatgcttttg acggagaaaa taatgaacgg
     2761 gcagatggct tctttctgga aacgctggtt ttcctttgag gcctggcttt ccatcagcga
     2821 aagcattatc aagcacggat atatttttcc ctcctttgat ggcaccgtct tctccctgga
     2881 aggtttttat cacccggttt tgaagaatcc cgtcaaaaat gatcttaccg cctcacatca
     2941 tgtgattctt ttaacaggcc ccaatatgtc cggcaagtct actttgctga aagcagtcag
     3001 tctctgcgtt tatctggcgc atgcagggct tgccgtgcct gcatcaaaag caaccatgcc
     3061 gttctttacc ggcatttccg tggccattaa ccttacggac agtatcgtaa gcggttacag
     3121 ccattttatg tcggaagtca ttactttgaa gaaagtgctc actaaagctg cgtccggcgg
     3181 aaaaaatttt gctgtttttg atgaactctt ccgcggaaca aatatcgagg atgcgctgga
     3241 cattagccgg gcaaccattc gggggatcgt ccattttcct gataccctgt tttttatttc
     3301 cacccacctg caccagctga aagagctgga ggaaatcaag acccatcggg tatccacctg
     3361 ctttattgaa tgtgagctac aggataatat tccggtgttc acctataaat taaaagaagg
     3421 ctggtccgat ttaaaactgg gtcgcattct ctttgaaaga gaaggattga ataccatgct
     3481 caacatatga ttttgcaaaa gcctaacttc atcaaaagaa taatattgca accatgctga
     3541 aacgatgcat ccgttggtcc tgcttaatag gaacgggatt cttcctgatc tgtcaaagcc
     3601 tttttattcc ggatgcagcc ctcggaaagg agccattgcc accacagatc tccatcgtta
     3661 ccgattcgct ggccggtggc tgcgcccggc agggattgaa aaagctgacg gaaattttag
     3721 ccaccagaaa tatatcattt gaagaagtcg gttcgcccga acaggcaaag gggagaatga
     3781 tcctggtcac cggtcttgct tacgacggga gcattgcatc cggattacta agagccggtc
     3841 agcatacggt tgacagggca cctgaagcct tgacggtttg gaaaacacaa tgggtaaaca
     3901 aaaaagttat cgtcatcagc ggttacgatg agaccggttt aatgtacgcc ctgacggatg
     3961 tagcccggca gatcaggcag aatgccagtt ccggcgatcc gctgaaagaa gtgcaggaga
     4021 taacggaagc ccccgccatc cgggatcggg ccgtttccat gtataccatg aaccgcgcct
     4081 attgggaaag ccgcttttat gatgaaaaat attgggggca atacctcgat atgctttcgg
     4141 aaaaccggtt caattcgctg gtcatcgtct ttggctatga gaacggcgga tttcttgcgc
     4201 cctgctatcc gtattttttt aatgtggatg agtttcccga tgtgaaaatg gtgggactga
     4261 gtgcggagca acagaataaa aatctgctgg cgatgaacag attgatctgc atggcgcatg
     4321 agcggggaat acgggtaacc gccggcatct gggatcacat ctaccgcggc ggggtgcagg
     4381 gtgggggcat gccgggaacg gagaaggcgc ctgatgaacc cgtagccggt ctggtatggg
     4441 gtctttccgc taataatctt accgcctata ccaaagcggc cctggaaaaa tttatccggg
     4501 aaattccggt ggatggcatt caattccggt tgcacggcga atcgggcctc cggaaagaag
     4561 aggaagcctc tttctgggac gatgtgttcc ggatgataaa aaccgtcaaa ccggatctgc
     4621 tggtggatct ccgggcaaag gaacttcctc cgacagccat accaagtgcg gagagcgcag
     4681 gaataaaatt tcgtatcgac accaaattct ggatggagca aatggggctg ccttatcatc
     4741 ccacgcggat caatcccgat ccgagttatc tccggcacag ctatggcgac ctgctccggt
     4801 atccgaaaaa atatgacatg aactggaggc tctggacggg agggacctcc cgcattcttc
     4861 tatggggcga tccggattat gcacgccgtt ttatggagag cgttcatctc tatgacggca
     4921 gcagctacga tgtaaacgaa ccgctggcta caaaaatgga agcccagccg gaaggaatac
     4981 cgccctttga tctgctgcag ccgcagcacc ggtactatac ttatgaattt gaacgctatt
     5041 ggcatttctt ccaggtattc ggtcgcctgg ggtatgatcc gcaggcatcc gcggccatct
     5101 gggacaaaga gcttgccctt cgcttcggga aaaaaacagg accgttggtg gaatctgctt
     5161 tgcacaaagc cagcgccata ctcccgcgca tcatcgcctc ctgttatccc tacggcgctt
     5221 tcccggccac gcgtggctgg gcggaaaaac agcacctggg cgatctgccg tattacgcaa
     5281 aagcagaagg cagcgatctt tgtcagtttg ccaattttga cgaggaggcg cggctcctgc
     5341 tggaaggagc cggaacggcg aaaataagac cctctgccaa ctgccaatgg ttcgcaaaaa
     5401 aagcgaagga tatcagggcc ctggttttac aaataaaggg acaaagcccg gaccgccgca
     5461 aggaattgaa ttccactatc acggatctgg agatcctggc caatctcgcg ctgtatcatt
     5521 ccgaaagaat accggcagcc gtttactacc ggttgtttgt acgaaccgaa gatgttgctg
     5581 cgctggattc cgcgattgcc tacgaaaaga aagctacaga agcctggcgg aaaatcgttt
     5641 cctcggcagg agattattat gactataatt tgcgaatggg cgtaagcgtt gcgggacttt
     5701 ccggccactg gagggatgaa ctt
//
LOCUS       DASYCU010000206         7787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3527149, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000206 DASYCU010000000
VERSION     DASYCU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527149"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGM24_04160"
     CDS             <1..208
                     /locus_tag="VGM24_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4154589.1"
                     /translation="VIVQLTRADRKVLITVEDDGTGFELHAPKKASGMGLHNVRSRVN
                     YLQGRMDIESRPGEGTIINIELIV"
     gene            212..835
                     /locus_tag="VGM24_04165"
     CDS             212..835
                     /locus_tag="VGM24_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4154590.1"
                     /translation="MTRVFIVEDHSVVVEGIRVLLQNENDIVVAGSSLTAAGCIDYFA
                     HQVADVVLMDINLPDMSGVELCRIIKIKCKEAMVLMLSTFNQGIYMNKAMENGASGYL
                     LKNVTRQEMIDGIRTVSRGGVYFSFEAGKIYKSSLESNSLRPVLTKREKEILKLIVEG
                     LTNSQIGRQLFISIDTVDTHRKNLYAKLNVKNTAQLIRYTIENGIIS"
     gene            1011..1403
                     /locus_tag="VGM24_04170"
     CDS             1011..1403
                     /locus_tag="VGM24_04170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY4154591.1"
                     /translation="MKKTFLLGIIMTLLAMTQSVAQDKKQVVRIARIEIDPAQADQYK
                     AAVKEIGETSLRSEPGVLMLFSVADKNQPGHITIIEIYADSAAYQAHLKTAHFKRYKA
                     ITRNMVRSLDLVEVDPVYAGIKPEIIHL"
     gene            complement(1424..2428)
                     /locus_tag="VGM24_04175"
     CDS             complement(1424..2428)
                     /locus_tag="VGM24_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4154592.1"
                     /translation="MNRSKIKFPPGYFFWIPLIGIIIISCNQNGQQTQKQDTQTAQTT
                     TSVSIASKEIPIWTKSIPDAGLITGTETHIDGMVSHVSNPTITIYSPKGKNTGAAVIV
                     FPGGGYIRLAIDLEGSQICEWLASIGVTGILLKYRVPASGPHYDKDCHCEKDPVKPLA
                     LQDAQRAMGMVRFRSKEWNIDPGKIGVMGFSAGGHLVADVSTNYRKRAYSVMDETDQT
                     SCRPDFGIAMYPGHMTIHTSKPFQLNSKLPVDSQTPPTFLLQAGNDNVDSIQNSLVYY
                     IALRKAGVKTEYHIYAEGGHAFGLNESAQKIPNWSELPIAGWENLVERWLRTIKMTSN
                     "
     gene            complement(2469..2933)
                     /locus_tag="VGM24_04180"
     CDS             complement(2469..2933)
                     /locus_tag="VGM24_04180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4154593.1"
                     /translation="MKAARRSAIKKLMVSLAGLTGLGALAKAGTAVRSGKQVQDITYQ
                     DQVPLFSGVTRHGDLLYVAGKGAHVAPFEIKAHTEIVLKELEETLVKAGSSMEKVLKV
                     SVFLNDIADYAGMNEVYKGRFGEHPPVRTTVAVAKGGVPGNSLVEMDCIAYV"
     gene            complement(2854..4623)
                     /locus_tag="VGM24_04185"
     CDS             complement(2854..4623)
                     /locus_tag="VGM24_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HEY4154594.1"
                     /translation="MNWTQVVDPLHNVYLSALVAVLPIIFIFWALVIRKMKGHQASIL
                     AMLLALLIAVMVYRMPVQMALLSTLQGALYGLFPICWIVVSILFLFNLIVKSGQLAII
                     RHSMAAITSDQRLHALLIAFSFGSFLEGTAGFGAPVAISAAMLAGLGFEPMYAAGICL
                     IANTAPVAFGAIGIPITVASQITGIPEMVLSQMVGRTLPLLSFILPFYLIVIMSGFKK
                     SLEVLPAILVSGISFAFLQWLCSNYSGPHLPDIIAGGGSILCLVLFLKGWQPKKIWRF
                     HQLAGPEQETKKVYSTRQIFRAWSPFILLTVMIILWAMQPVARMLNKATLLIPVAGLD
                     QRITDFNGNLLPHVFKLNILSAAGTAIFISALLAIPLTGLRMREGAKIFIGTLRQLKW
                     PIFTVSAILGFAYIVNDSGIAISIAHALARTGFLYPFFAPVLGWLGVFITGSDTSSNA
                     LFSKLQYATATSVGINPVVTVAANSTGGVVGKMISPQSIAVATASVGLTGKESSLFRF
                     TLKHSLIILGMICLLTFAQAYYIKWIVPPYPAPSYGNKMHNPADSADHLKIKTYHHES
                     SKKIGHKETDGLLSRPHRAGRTS"
     gene            complement(4652..6280)
                     /locus_tag="VGM24_04190"
     CDS             complement(4652..6280)
                     /locus_tag="VGM24_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4154595.1"
                     /translation="MKRRELIKNLSILPLAGTIIGNNDPMQSRPGINGYGKGAEGLAN
                     QAGATRGIYASLGVRPVINGRGTITIIGGCRVLPEVENAMREATLDYVELDELMEGVG
                     KRLGELTGSEWGVVTTGATGALIIGTLGVITGGNPDKLWQIPDLRGMKNEVIIPAYSW
                     TAYESAVRGIGVKMVTVEDKEELIAALGPQTAMILVLAGARSMNGPLSLQEISAIAKP
                     LGVPVMVDAAAEGLPVPNPHISRGADLVAYSGGKYLSGPQCAGLLLGRKDLMQAAWMT
                     SAPHHGFARGYKVGREEVLGMLTAVEMWMKRDHAKEIETWTNQLKKIDDQIKKIPGVT
                     TSIHQPRPDQLSNPSPGLRVSWDMNKIPLTGDDLEQILWNGNPRVSVGGAGSFLPFPP
                     NMQPNVHVNTSQLREGEERIIAEQLYKALSNPPHLEKPSGAPAFNISGEWDLEIQFAA
                     GTDRQTLIFEQKENELNGTHIGSYAKRDLAGTMFGNKILFRSAHTQNGVRLDFEFTGT
                     ADSSDSMGGALSLSEYGMASWKAKRHHYQLRGAH"
     gene            complement(6301..7764)
                     /locus_tag="VGM24_04195"
     CDS             complement(6301..7764)
                     /locus_tag="VGM24_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154596.1"
                     /translation="MKAKIKSIKIYRPLFLSLLLCLGGCSKSFLDVQPPSVLTGASYY
                     RNATDAEAAITAAYSSVRNFNTDNYAKITEAPLTDMMIFNTQGLNLDSWSLDPNDVIP
                     DDVWQGAYEGIFRANLVLQHVPDISMDAAEQSRILGEAYFLRALFYWQLSTVFGPVPI
                     VPEADPSDPSKASLPNNSVDEINNGMIGDLQMASSLLPKRSGYGSADLGRATKGAAQA
                     LLGKVYLYAQNYPLAETYLDSVITSGEYQLIPSFGDLLVTDNNAESIFEIQYADITGQ
                     GSDRIENDYPQGQGGFSNLLPTQELVDAFESSTGSTAVNGRDPRLFYSVFQDGDPYDA
                     VSPVFKNSWTPTGYARKKGSFPVVRTSNANLGRDFPIIRLADVLLMYAEAANENGHAP
                     EAIAAINKVRERVGMEDLPTVQFPTGTKEEIFAAVVHERTVELAFEHTRLNDLQRWGM
                     ATQVLGGAGYTAPKNRYFPIPQQEINNNPQLVQNEGY"
BASE COUNT         1862 a         1969 c         2046 g         1910 t
ORIGIN      
        1 ggtgattgtg cagctgacca gggccgacag gaaggtactg atcaccgttg aggatgacgg
       61 aacaggattt gaactgcacg caccgaagaa agcaagcggt atgggtttgc ataatgtgcg
      121 atccagggtc aattatctgc agggccggat ggatatcgaa tccagaccgg gcgaaggcac
      181 cattataaac atcgagttaa ttgtataatg catgacgcgg gtttttatcg tggaggatca
      241 ctcggtggtc gtcgagggca tccgggtatt gctgcaaaat gaaaatgata ttgtggtagc
      301 gggatcttcc ttgactgccg cgggatgcat cgattatttt gcgcatcagg tcgcggacgt
      361 ggtattgatg gacatcaacc tgcccgatat gagcggggtg gaactctgcc ggatcataaa
      421 aataaagtgc aaggaagcga tggttttgat gctcagtaca tttaaccagg gcatttatat
      481 gaataaagca atggaaaatg gcgccagcgg ctatttgctg aagaatgtaa cccgccagga
      541 aatgattgac gggatcagga ccgtaagccg gggtggggta tatttcagtt ttgaagccgg
      601 caaaatatat aaatccagcc tggaaagcaa cagcctccgg ccggtactca ccaaaagaga
      661 aaaggaaatt ttaaaattga ttgtggaagg gcttaccaac agtcagatcg gccggcaatt
      721 attcatcagc atcgataccg tggacaccca ccggaaaaat ctatatgcca aactaaatgt
      781 aaaaaacacc gcccagctga tccgctatac cattgagaat gggatcatat cctgacccgc
      841 tgcgaccagt cataactcta atggctgttg aaatatatcc gacgtaaaac ccccgtaaaa
      901 tttattaaat ttataatttg aaagaagaaa tactgatgct acgggcgaaa gttcagcagg
      961 aagatttaaa agcgatcacc ggagcaccct gaatgacctc aaccaggctt atgaaaaaga
     1021 ccttcttatt aggaatcatt atgaccctgc ttgccatgac acaaagcgtt gcacaggata
     1081 aaaaacaggt agtccggatt gccaggatag agattgatcc ggcacaggca gaccaatata
     1141 aggcggcagt aaaagagatc ggtgaaacct ctttaaggtc ggagccgggc gtattaatgc
     1201 ttttctccgt cgcagacaaa aatcagccgg gccacattac gatcatagaa atttatgccg
     1261 atagcgcagc ctatcaggcg catctcaaaa cagctcattt taaaaggtac aaggcaataa
     1321 cccggaacat ggtcaggtcg cttgatcttg tcgaggttga ccctgtttat gccggcataa
     1381 agccggaaat tatacatctt tgaacagctg ttccgcgcta ttcttagtta gaggtcattt
     1441 ttattgtgcg taaccatctt tcaaccagat tctcccaacc ggcaatgggg agttcggacc
     1501 aattgggaat tttctgcgcc gattcattta agccaaaagc gtgtccgcct tcggcataaa
     1561 tatggtattc ggtttttaca ccggcttttc taagtgcgat ataatacact aatgaattct
     1621 gtatggaatc gacattgtca ttgccggcct gcaacagaaa tgtgggaggg gtttgactgt
     1681 ccaccggaag cttgctgttt aactggaaag gtttactggt gtgaatggtc atgtgtcccg
     1741 gatacatggc tattccgaaa tccgggcggc aactggtttg atctgtttca tccatcacgg
     1801 aataggctct ctttctatag ttggtactga catccgcaac caggtgtccg ccggctgaaa
     1861 aacccatgac gccgatttta ccggggtcga tattccattc cttggaacga aaacggacca
     1921 ttcccatagc tctttgggcg tcctgtaagg caagcggctt tacagggtct ttttcgcaat
     1981 ggcaatcttt gtcgtaatgc ggccccgatg caggaaccct gtatttcaga aggatgccgg
     2041 tcactccgat cgatgccagc cattcgcata tctgggaacc ttccaggtcg atggcaagcc
     2101 ggatataccc tccgccggga aatacaatta ccgcggctcc ggtgtttttc cctttgggtg
     2161 aataaatggt gattgtcggg tttgaaacat ggctgaccat cccatcaata tgcgtttctg
     2221 tcccggttat gagtccggcg tcgggaatgg attttgtcca gatcggtatt tctttactgg
     2281 caatggaaac tgatgttgtt gtttgtgctg tctgtgtatc ctgcttttgg gtctgttgcc
     2341 cgttttgatt gcaggaaata ataatgatgc cgattaacgg aatccaaaaa aaatacccgg
     2401 gcggaaattt aattttcgat cggttcattc ggaatgtcgt tcctgtaagt ttacgaaagc
     2461 tggatccttt agacataagc tatgcaatcc atttccacca gggagtttcc cggtacaccg
     2521 cctttggcga cagccaccgt ggtacgcaca ggcggatgtt ctccaaaacg tcctttgtat
     2581 acttcattca tgcctgcata gtcggcgata tcattcagga acacgcttac tttaagcact
     2641 ttctccatcg acgagccggc ttttaccagc gtttcctcca gttccttcaa aacgatttcc
     2701 gtgtgggcct tgatttcaaa gggagccaca tgcgcgcctt ttcctgccac atacagcagg
     2761 tcgccatgac gggtaactcc cgaaaataaa ggcacctggt cctggtaggt tatgtcctgt
     2821 acctgcttgc cggatcttac ggcagttcct gctttagcta gtgcgcccag cccggtgagg
     2881 ccggctaagg agaccatcag tttctttatg gccgatcttc ttgctgcttt catgatgata
     2941 tgttttaatt tttaagtgat ctgcggaatc cgcagggtta tgcattttgt tcccgtaaga
     3001 aggagccgga taaggcggaa cgatccattt tatataataa gcctgtgcaa aggtaagcag
     3061 gcagatcata cccaggataa tcaaactatg ttttagggtg aaccggaaaa gagacgactc
     3121 cttacctgtc agtcccacgg aggccgtggc cacggcaatg gattgcggcg atatcatttt
     3181 tcccaccacg ccgccggtag aattggcggc aacggtcacc accgggttaa tgcccactga
     3241 agtggcagta gcatactgca gtttggagaa caatgcattg gaagaagtat ccgatccggt
     3301 aatgaaaacg cccagccatc ccagcaccgg cgcaaagaac ggatagagaa atcctgtgcg
     3361 cgccagcgca tgggcgatgc tgatggcaat gcccgaatcg ttcacgatat acgcaaagcc
     3421 gaggatggcg gatacagtaa agatgggcca ttttaactgc cgcagcgtgc caataaatat
     3481 ttttgccccc tcgcgcatcc ggaggcccgt aagcggaatg gcaaggagcg cggagataaa
     3541 aatcgcggtg ccggctgcgg ataagatatt cagtttaaaa acatgcggaa ggagattgcc
     3601 gttaaagtct gtgatccgtt ggtcgagacc ggcaaccggg atcagcaggg tcgccttgtt
     3661 caacatgcgg gcgacgggct gcatcgccca taggatgatc atcaccgtta ggaggatgaa
     3721 cggcgaccag gcacggaata tttgcctggt tgaatagacc ttttttgttt cctgttccgg
     3781 acccgccagc tgatgaaaac gccagatctt cttgggctgc cagcctttga ggaagagcac
     3841 gaggcagaga atggatccgc cgcctgcaat gatgtccgga aggtgaggcc cggaataatt
     3901 tgaacataac cattgcagaa aagcgaagga aatgcccgat accaggatgg ccggcaatac
     3961 ttccagggat ttcttaaatc cggacattat gacgattaaa taaaatggaa ggatgaacga
     4021 taaaagcgga agcgttctgc cgaccatctg cgacagcacc atttcgggaa tgcctgttat
     4081 ctgcgaggcg accgttatag gaatgccgat ggccccgaag gctaccggcg ccgtgttggc
     4141 gatcaggcag atgccggctg catacatcgg ttcaaatcca agcccggcaa gcatcgccgc
     4201 actgatggcc acaggcgctc cgaagccggc cgttccttcc aggaagctcc cgaatgaaaa
     4261 agcgatgagt agggcatgca ggcgctgatc ggaggtgatg gctgccatgg aatgccggat
     4321 gatcgcgagc tgaccgcttt ttacaatcag gttaaacagg aacaggatgc tgaccacgat
     4381 ccagcaaatg ggaaacaatc catacagcgc gccctgcaga gtggagagca gggccatctg
     4441 cacaggcatc cggtaaacca tcaccgctat cagcagagca agcagcatgg ccaggatact
     4501 ggcctgatgg cccttcattt tccggatcac caatgcccag aaaataaaaa taatgggcag
     4561 aactgctacc agtgcggata aataaacatt atgcagcggg tcgacgacct gtgtccagtt
     4621 cattcaaaaa ttgcttattc tttctgcctt cttaatgcgc gccacggagc tgataatggt
     4681 gacgttttgc tttccaggag gccatgccgt attcactcag gctaagtgcg cctcccatgc
     4741 tgtcgctgct gtctgccgtg ccggtaaatt cgaaatcgag ccgcacgccg ttctgcgtat
     4801 gagcactccg gaaaaggatt ttgttcccaa acatcgttcc ggcaagatcg cgtttggcat
     4861 aactgccgat atgggtcccg ttcagttcgt tctctttctg ctcgaaaatt agggtttgcc
     4921 ggtcagttcc cgcagcaaat tgaatttcca ggtcccattc gccgctgata ttgaatgccg
     4981 gcgcgccgga aggtttttcc aaatgcggag gattcgataa agccttatac aactgctccg
     5041 ctataatgcg ttcttcgccc tcccggagct gggaagtatt cacatgaacg ttcggttgca
     5101 tgttgggagg aaaggggagg aagctgccgg cgccgccaac ggaaacgcgg ggattgccgt
     5161 tccataagat ctgttccaga tcatcaccgg tcaacggaat cttgttcatg tcccaactta
     5221 cgcgaagtcc ggggctggga ttggacaact gatcgggccg cggctggtgg atggaagtag
     5281 ttactcccgg gatttttttt atctgatcgt caattttctt tagctggttt gtccaggttt
     5341 ctatctcttt cgcatggtca cgcttcatcc acatttcaac ggcagtgagc atgcccagca
     5401 cctcttccct tcccacttta tacccgcggg caaaaccgtg atggggagcg ctcgtcatcc
     5461 aggctgcctg catcaggtct ttacgcccga gcagcagacc cgcgcattgc ggtccgctta
     5521 aatattttcc accactatag gccaccaggt cggcgccccg gctgatatgc ggattgggaa
     5581 caggcaggcc ttctgcggcg gcatctacca taacgggaac gcccagtggc tttgcgatgg
     5641 ccgaaatttc ctgtaacgac aggggtccgt tcatggatct tgcaccggcc agcacgagga
     5701 tcatggccgt ttgcggtccc agggctgcga tgagttcctc tttatcttcc acggtaacca
     5761 tcttgacccc gattccccgt acggccgatt cataagcggt ccaggaatac gccggtatga
     5821 tcacttcgtt tttcattccc ctgagatccg ggatctgcca gagcttgtcc gggttgcctc
     5881 ccgtgatcac acccagcgtg ccgatgatga gtgcaccggt tgcaccggtt gttaccacac
     5941 cccattccga tccggtgagt tcacccagcc gtttgcccac gccttccatc aattcatcca
     6001 gctccacgta gtcgagcgtt gcttcccgca tggcattctc tacttcgggc agcacgcggc
     6061 agcctccgat gatggtgatg gttccccgtc cgttgatcac aggacgcaca cccagggatg
     6121 cgtagatacc ccgggttgcg cctgcctgat ttgccaatcc ttcggcccct ttaccgtaac
     6181 cattgattcc gggccgcgat tgcatagggt cattgtttcc tattatggtt cctgccagcg
     6241 gcagtatgct cagatttttt atcagctctc tgcgtttcat aattaaaaat tgtaaatgaa
     6301 ttaatacccc tcgttttgaa ccagctgcgg gttgttgttg atctcctgct gcggtatcgg
     6361 aaagtagcgg tttttgggcg ctgtatatcc ggcgccgccc aatacctgtg tcgccattcc
     6421 ccaacgctgg agatcgttca gccgggtatg ctcaaaggca agttccacgg tgcgttcatg
     6481 cacgacggcg gcaaagatct cttctttggt tccggtcgga aactgcaccg tcggcaggtc
     6541 ctccatgccc actctttccc gcaccttgtt gatggcggca attgcttccg gagcgtgtcc
     6601 gttttcattg gccgcttccg cgtacatcaa cagcacgtcg gcgagacgga taatgggaaa
     6661 gtcgcggccg aggttggcat tgctcgtgcg caccacgggg aaagatcctt ttttccttgc
     6721 ataccccgtg ggcgtccagg agtttttgaa tacgggagaa acggcgtcgt agggatctcc
     6781 gtcctgaaaa acagaataga acaggcgggg atctctgccg ttgacggctg ttgacccggt
     6841 gctgctttca aatgcatcca ccagttcctg tgttggaagc aggttggaaa acccgccctg
     6901 tccctgcggg taatcattct cgatgcggtc gcttccctgg ccggttatat cggcatactg
     6961 gatctcgaaa atagattccg cattgttatc ggtcaccagc agatcgccga aggaagggat
     7021 cagctggtat tctccggagg tgatcacgct gtccaggtag gtttccgcca gcggataatt
     7081 ctgcgcatac agatacactt tacccagtaa agcctgggca gctcctttag tggcgcggcc
     7141 gaggtccgca ctgccgtatc cggaacgttt cggcagcagg gaggaagcca tctgcagatc
     7201 accgatcatc ccgttattta tttcgtccac gctgttgttg gggaggctcg ctttggaagg
     7261 atcggaagga tccgcttccg gcactatggg cacgggaccg aaaacggtgc tgagctgcca
     7321 gtagaacaac gcgcgcagga aataggcttc ccccaggatg cggctttgtt ccgcagcatc
     7381 catgctgata tcaggcacat gctgcagcac caggttggcg cggaatatgc cttcgtatgc
     7441 accctgccat acatcatcgg gaatgacgtc gttcggatcc aggctccagg aatcgaggtt
     7501 cagcccctgg gtattgaaga tcatcatatc ggtcagcggc gcttcggtga tcttcgcata
     7561 gttatccgtg ttaaaattgc ggaccgaact gtaggccgcg gtgatggccg cttcggcatc
     7621 ggttgcattc cggtaatacg aggcccccgt caatacgctg ggcggctgca cgtccaggaa
     7681 gctcttgctg catcctccca ggcagagcag gagagacagg aatagaggcc tgtatatttt
     7741 tatcgatttt atttttgctt tcataacagg ttaaaattta aaagtca
//
LOCUS       DASYCU010000207         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4105078, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000207 DASYCU010000000
VERSION     DASYCU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4105078"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGM24_04200"
     CDS             complement(<1..208)
                     /locus_tag="VGM24_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154597.1"
                     /translation="MTRHAWLAALLVVSYQTVSAQTATTPPPAAQQQPPEAPFRKSPT
                     VPEFSLIKADSIGYITKEDIKKHHP"
     gene            358..723
                     /locus_tag="VGM24_04205"
     CDS             358..723
                     /locus_tag="VGM24_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY4154598.1"
                     /translation="MEKLTKQEEEAMLAVWKTGEGHVKGFLENMDEPRPPYTTLASTI
                     KNLEKKGYLGSRLLGNAYLYKPLITEEEYKKKFMNGFVRDYFDNSYKELVNFFVDQKK
                     LSARELKEIIALIEGRKGE"
     gene            761..>1583
                     /locus_tag="VGM24_04210"
     CDS             761..>1583
                     /locus_tag="VGM24_04210"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY4154599.1"
                     /translation="MPLLIIYSLKFSIGLAVAWLFYQGVLRRLTFYSWNRWYLLGYTS
                     LCFFIPLINVQRVVESGPFTTPGIIRYIPVFQLPARDHSWDLVIFLLVAVSAILLIRL
                     VIGWLSVRKMRRHATPVSGLAMTGKSDPERMQIYQVEEKIVPFSFGNAIYINQDLHTE
                     KEYEEIILHEFVHIRQRHSVDILAAELMCVFAWYNPFAWLIRHSIRQNLEFIADSKVL
                     EKGFDKKGYQYHLLKVIGEPKYRLATNFNFSSLKKRIVMMNKIRSARLHLVKFLFV"
BASE COUNT          391 a          311 c          443 g          438 t
ORIGIN      
        1 cgggatgatg ctttttgata tcctcttttg tgatatagcc gatgctgtct gccttgatca
       61 ggctgaattc gggaacggtg ggtgatttcc tgaaaggggc ctcgggaggc tgctgctggg
      121 ccgcgggcgg tggtgttgtg gctgtctgtg cggatacggt ctggtaactg actactaaga
      181 gtgctgcgag ccatgcgtgt ctggtcatct tgcggttttt tcagaataat aattgcccgg
      241 tgcaggtgtc tctggaaaga ccgggcggcg actgcaaaag taaatgttta acgaaaaaaa
      301 taagttttta tttgttttat tacgaaatat tcgtaggttt acgaaaactt cgtaataatg
      361 gaaaaattaa ccaaacaaga agaagaggcc atgctggcgg tgtggaagac cggggaggga
      421 catgtcaagg gattcctgga gaatatggat gaaccaaggc ctccttatac caccttggcc
      481 tcgacgatca agaacctgga aaaaaagggt tatctcggaa gtcgtctgtt ggggaatgcc
      541 tatttgtata agccgttgat cacggaggaa gagtataaaa agaaattcat gaatgggttt
      601 gtgcgggatt atttcgacaa ttcatataag gagctggtga attttttcgt ggatcagaaa
      661 aagctgagtg cgcgggagtt gaaagagatc attgcgctca tcgaggggag gaagggggag
      721 tgagaacgaa tgaattcagg tcacattaaa aattgcgtgt atgccattac tgatcattta
      781 cagtttaaaa ttttcgatag gtctggctgt ggcctggttg ttctatcagg gggtgctgcg
      841 acggttgacc ttctatagct ggaatcgctg gtatttgctg gggtatacgt ccttgtgctt
      901 ttttatccca ttgatcaatg ttcagcgggt cgtggaaagc ggccccttta cgacgccggg
      961 gatcatccgg tatattccgg tcttccagct gcccgcgcgc gatcattcct gggatctggt
     1021 gatcttccta ttggtggcgg tgtcggcgat ccttttgatc cggctggtta tcggatggct
     1081 atcggtccgg aagatgaggc gtcacgcgac gccggtcagc ggtctggcca tgacaggaaa
     1141 gagtgatccg gaacggatgc agatctacca ggttgaggaa aagatcgtgc ctttctcctt
     1201 tgggaatgcc atctatatca accaggatct gcataccgaa aaggaatatg aggagatcat
     1261 ccttcacgaa tttgtgcata tccggcagcg gcatagcgtg gatattctgg cggccgagct
     1321 gatgtgtgta tttgcctggt acaatccctt cgcctggctg atccgccatt ccattcgtca
     1381 aaatctcgaa tttattgccg atagtaaagt cctggaaaaa ggctttgaca aaaagggcta
     1441 tcaatatcat ttattaaaag tgatcgggga gccgaagtac cggctggcca ccaatttcaa
     1501 tttttcatcc ttaaaaaaac gcattgttat gatgaacaag atcaggagtg cccgtcttca
     1561 cctcgtgaaa ttcctttttg tat
//
LOCUS       DASYCU010000208         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2365340, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000208 DASYCU010000000
VERSION     DASYCU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365340"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VGM24_04215"
     CDS             <1..583
                     /locus_tag="VGM24_04215"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154600.1"
                     /translation="GLMTDWGVHLLDMALWVKNIQTPPLAVMTAGGNFSFPDHAHETF
                     DTMSVCYQMPDYTINWEHTAGTQLGPYNRSYGLAYIGDNATLVIDRDGWELFPEKEGS
                     GFRTPAIPKQPGKDSHEEHMKNFLECIRTRKDPACTIENGRLVAMYAHMGNMALRTNS
                     RLEWQEQSRNFGNNRQANALLMPAYRKPWELKL"
     gene            complement(589..1641)
                     /locus_tag="VGM24_04220"
     CDS             complement(589..1641)
                     /locus_tag="VGM24_04220"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4154601.1"
                     /translation="MSIRYFLYLGVFALCFGSCNNASETGRQTAENKADSVTPKKTAR
                     RIVILDPEALTILDSSALIEVIASGFQWAEGPLYVRDGNYLLFSDVPANRIYKWQEGK
                     DTSVFLDHSGTAGPIRDPKEPGSNGLVLNNQGQLVLCQQGDRRMAKMTAPLSDPKPQF
                     IPLAAAYQGKRLNSPNDAVYHPNGNLYFTDPPYGFSKGIRDSAKQLPFQGVFCLKPDG
                     RLLLLTDELSYPNGIALSPDGKYLFVSNSDDRNKLWMKYELDRNGLIKSRQVFYHATE
                     YDGRDLGNPDGMKINKKGYLFAAGPEGIWLFNPSGKLIAKILTGELTSNCAFSSGEKE
                     LFMTCNHYIMRCRLRG"
     gene            complement(1645..2616)
                     /locus_tag="VGM24_04225"
     CDS             complement(1645..2616)
                     /locus_tag="VGM24_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008671 - 2-dehydro-3-deoxygalactonokinase
                     activity [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY4154602.1"
                     /translation="MSHFLSCDWGTSSFRLKLVDSSSQTALAEVMSHQGIAVTHQDWL
                     NQKLPEDKRFDFYLDFLKRKIHELEEKGKHSLKNIPLLISGMASSSIGMMVLDYLPMP
                     FPCDPENIGTKLIEASASFSHPLILISGVKTEQDVMRGEETLLIGCDTEATEQYFIFP
                     GTHSKHILVKNGSAVGLKTYMTGEIFELLARHSILSASLAEHIREDAIRDSLHFAKGV
                     NDGMGSNLLNAVFKVRTNQLFKKSSPEENYLYLSGLLIGYELSDLKNSTHTVMLVSGP
                     NLKEYYRAALISSGFELTLQDADQALIRGQYRIAAQILKPSLSPDKS"
     gene            complement(2635..>3085)
                     /locus_tag="VGM24_04230"
     CDS             complement(2635..>3085)
                     /locus_tag="VGM24_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4154603.1"
                     /translation="GILFSGFLSDHLMKKGVPAGRARKIPVITGLLLSISIVGSNFVS
                     STFLITLFMTIAFFGNGLASITWIFVSAIAPKHLLGLTGGVFNFAGGLAAIIVPVAIG
                     YLVQNGDFSPALVFIGCIALTGALSYLFLVGKVERIKTENDPVFISS"
BASE COUNT          819 a          742 c          751 g          773 t
ORIGIN      
        1 cggattgatg acagactggg gcgttcattt gctggatatg gctttatggg ttaaaaatat
       61 tcagaccccg ccgctggccg ttatgactgc gggcggtaat ttttcttttc ccgatcatgc
      121 gcacgaaacc tttgacacca tgagtgtgtg ttatcagatg ccggactata cgatcaattg
      181 ggaacacaca gccggcacac agctcggccc atacaaccgt tcctacggac ttgcttatat
      241 cggagacaac gccaccctgg ttattgaccg ggatggatgg gagttattcc cggaaaaaga
      301 aggttcgggt ttcagaactc ccgccatccc caaacaaccc ggaaaggatt cccatgaaga
      361 gcacatgaag aatttcctgg aatgtatcag gacccgcaag gatccggcct gcaccatcga
      421 aaacggcagg ctggtagcca tgtacgcaca catgggaaat atggccttgc ggaccaattc
      481 gcgtctggaa tggcaggagc aatcgcggaa tttcggaaat aaccggcagg ccaatgccct
      541 ccttatgccg gcctaccgga aaccctggga attgaaatta tgatccggtt atccccggag
      601 ccggcaacgc atgatataat gattgcaggt cataaaaagt tctttttcac cggaagaaaa
      661 agcgcaattg gacgttagtt cgccggtcag tattttcgca atcagttttc ctgacggatt
      721 gaacagccaa atcccttccg gcccggcggc gaacagataa ccctttttat ttattttcat
      781 tccatccgga tttccgagat cccggccatc gtattccgtg gcgtgataga aaacctgtct
      841 gcttttaatc aatccgttcc ggtccagctc atatttcatc cagagtttat tccggtcgtc
      901 tgaattggaa acgaacagat attttccgtc cggcgaaagg gcaattccgt tcggataact
      961 aagttcatcg gtcagcagca ataatctgcc atccggtttt aaacaaaaca ccccctgaaa
     1021 agggagttgc tttgcgctgt cccggattcc tttgctgaat ccatacggcg gatcggtgaa
     1081 atataaattt ccattcgggt gataaacagc gtcattcgga ctgtttaaac gcttaccctg
     1141 ataagcagct gcaaggggta tgaactgtgg cttcggatcg gaaagaggag cggtcatttt
     1201 tgccatgcgc cggtctccct gctgacataa aaccaattgc ccctgattat taagcaccaa
     1261 tccatttgat ccgggttctt taggatcccg gatgggccct gcagtgcctg aatgatccag
     1321 aaaaacggaa gtatccttac cttcttgcca tttgtaaatc cggttagcag gaacatcgga
     1381 aaataacagg taattgccgt ccctgacata aagcggtcct tcagcccact ggaagcccga
     1441 cgcaattacc tcaataaggg cagaagaatc caggatagtc agggcttccg gatcgagaat
     1501 aacaattctt cttgctgtct ttttgggggt aacggaatcg gctttatttt ccgcggtttg
     1561 tctgccggtt tcactggcat tgttacagga accaaaacac aaggcaaaaa caccgaggta
     1621 aaggaaatag cgaattgaca tacattagga tttatcgggt gaaagggatg gttttaaaat
     1681 ttgagcggca atccggtatt gcccgcggat cagggcctga tctgcatcct ggagcgttaa
     1741 ctcaaaaccg ctgctgatca gggctgcgcg gtaatattct ttcaggtttg ggccactcac
     1801 cagcatgacc gtatgggtag aatttttcag atcgctcagt tcataaccga tcagcagtcc
     1861 gctgaggtac agataattct cctccgggga tgatttttta aacaactggt tggtacggac
     1921 tttgaatacg gcattcagga gattcgaacc cattccgtca ttaactcctt tggcaaaatg
     1981 gaggctgtcc cggatcgcat cctcccggat atgttcagcc agtgaggcgg ataagatgct
     2041 gtggcgggcc agtaattcga agatttcacc ggtcatatag gttttaagac cgacggcgct
     2101 gccatttttt accaggatat gtttggaatg ggttccgggg aaaataaaat attgttcagt
     2161 agcctccgta tcacagccga tcaaaagggt ttcttctccg cgcatgacat cctgctctgt
     2221 tttgacgccg gagatcagga tcaggggatg ggagaacgag gcagacgctt cgatcagttt
     2281 ggtcccgata ttttcaggat cgcaagggaa aggcatcggc agatagtcca ggaccatcat
     2341 tcctatgctg gaggaagcca tgccggagat cagcaacgga atatttttca gggaatgctt
     2401 tcctttttct tccagttcat gaattttccg ttttaagaaa tccaggtaaa aatcaaaccg
     2461 tttatcttcc ggcaatttct gattcaacca gtcctgatgc gttaccgcaa taccctgatg
     2521 gctcatcact tcagctagtg cggtttggct ggagctatcc accagcttta gcctgaaaga
     2581 agaagtaccc cagtcgcaac tcagaaaatg gctcatacgg cgaagaatta tttattatga
     2641 tgaaatgaag accggatcgt tttccgtttt aatcctttcc actttgccta ccaaaaacag
     2701 gtaggacagg gccccggtca atgcgatgca cccgatgaaa accagcgcag gggaaaaatc
     2761 tccgttctga accaggtagc cgatcgcaac aggaacaatg atcgcagcca acccgccggc
     2821 gaaattaaat acccctccgg tcaatcccaa taaatgcttt ggggctatgg cagaaacaaa
     2881 tatccaggtt atggaggcca gtccgtttcc gaagaaggcg atggtcatga aaagagtgat
     2941 caggaaggtg ctgctcacga aattggaacc gacaatggaa atggacagca gcaatccggt
     3001 gatgacgggt atttttctgg cccttccggc cggcacgcct tttttcatca gatgatcaga
     3061 taaaaaaccg gaaaataata ttccc
//
LOCUS       DASYCU010000209         4196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5123000, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000209 DASYCU010000000
VERSION     DASYCU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5123000"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VGM24_04235"
     CDS             <1..388
                     /locus_tag="VGM24_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEY4154604.1"
                     /translation="ASKAAAPIFVDAVKKMTVQDGLTILRGADTAATGYLRKTTSPQL
                     TAAFHPVIDSSLQKTGATKYWKNVFDTYNKLPTTFKKVDPDLSAFVTQKALDGIFYYV
                     AQEEMKIRKDPAAQVTDLLKKVFGGK"
     gene            complement(396..1601)
                     /locus_tag="VGM24_04240"
     CDS             complement(396..1601)
                     /locus_tag="VGM24_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4154605.1"
                     /translation="MKMINPFSSTLTLYKNAYSGLSRSTWLLSVVMLVNRSGTMVLPF
                     MTIYLTAMGFSLFHAGLVVGLFGLGAICGGYLGGKLTDKWGFHQIQLVTLCGGGLLFI
                     VLGLMRHYLLICLFTFILSLVNEAFRPANNTAIAHYSKEENRTRSFSLNRLAINLGWA
                     FGGSLGGFIASFNYHLLFWIDGLTNITAAFLLHYLLKSKPVAEPEHRPNHDLVPARSA
                     FHDKTYILFISLAILFGMCFFQLFSTLPVYYKDHLQLSEFHIGLLMGLNGMIIVLIEM
                     VLVFRLEAKKKITRNISWGLLLTSFSFIVLNLLPPQPYSALISVILVTFGEIFAMPFM
                     NTYWTSRTLPANRGQYAGLYTMAWSLAQVAGPTGGTWIAGHYGFYTLWWIAGGICLLT
                     AILFRTAKL"
     gene            1703..3358
                     /locus_tag="VGM24_04245"
     CDS             1703..3358
                     /locus_tag="VGM24_04245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY4154606.1"
                     /translation="MQRILVVLFLLLFGFGIGMPKVRAQEEDYSKHGWQLMDYRDDGL
                     FGAGVTRAYRELLKGKKSHLVIVAVIDDGIDTAHEDLAGHIWTNKKEIPGNGKDDDHN
                     GYIDDVHGWNFLGGKDGRNITVESYESYREYYRLRNSRSAGSMAAKDPGNAYWIKVRN
                     GFLKDSIREAHTVSMVGQMIPQMRFTDSLLRAVLHKDSVNAWDLEALVPKDSLVRQAK
                     KSGLKYFRRYGISSDMPLGKFIVEAGKYLESAKLVLNSFSEDPNAMRSEIVKDDFNNI
                     DDRNYGNNNITEGTSSHGTHVAGIIAASRNNGIGIDGIADNVQIMPIRAVPQGDERDK
                     DVALAIRYAVDNGARIINMSFGKYFSPGKKWVDDAVKYAEEHDVLIVHIAGNESLDID
                     TVSNYPNPDEPGGRRKSSAFITVGASTRGPDSLVVARFSNYGRKAVDLYAPGVKIYST
                     VPGNQYEAYSGTSMAAPVVSGIAALLLEYYPKLSAGQLKYILTHSVMKLSHPGVRLAA
                     TGKTVDFKTLSASGGIVNAYNALKLAGSLKGKTGKAFRMKRMR"
     gene            3496..3750
                     /locus_tag="VGM24_04250"
     CDS             3496..3750
                     /locus_tag="VGM24_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154607.1"
                     /translation="MLPHIDNQQDRLAEIFRQMDNQVTQESKVAFLKIQSWQFAVRTP
                     EIGPTYLREAASMITQSLLSFVPNSFVLSEEGYYLNPIEN"
     gene            complement(3772..>4196)
                     /locus_tag="VGM24_04255"
     CDS             complement(3772..>4196)
                     /locus_tag="VGM24_04255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY4154608.1"
                     /translation="RDEVSGSASEAKVPEFIEFIVKDMPEHQTPMRGGLRWLDMQCLT
                     RFGKSFKDCTHEQQMEMVDEIAWPKKAKPNNRPGVAFFNLVRNLVATGFYTSEIGVKD
                     VGYIGNVPSQWNGVPDDVLKQYNMAYTEKELKECVSFS"
BASE COUNT         1159 a          968 c         1098 g          971 t
ORIGIN      
        1 tgcatccaaa gcggctgcac ccatattcgt agatgcggtg aaaaagatga cggtgcagga
       61 tggcctgact attttgcggg gtgccgatac ggctgccacg gggtatctcc gcaaaacgac
      121 cagcccccaa ttgacggccg cttttcatcc ggtgattgat tcctctcttc aaaaaaccgg
      181 ggcaacaaaa tactggaaga acgtttttga cacctacaat aaattgccaa ctacttttaa
      241 gaaggtagat ccggatctat ccgctttcgt aacccagaaa gcgctggatg ggatcttcta
      301 ttacgtagcc caggaagaaa tgaagatccg caaggacccc gctgcgcagg taaccgacct
      361 gttgaaaaag gtattcggcg gaaaataatg cttctttaaa gctttgcggt gcggaaaaga
      421 atggcggtaa gcagacaaat gcctccggca atccaccata gagtatagaa cccgtaatgc
      481 ccggcaatcc aggttccacc agttggtccg gccacctggg ccagggacca ggccatggta
      541 taaagtcctg catattgtcc acggtttgcc ggaagggtcc ggctggtcca gtaggtgttc
      601 ataaaaggca tggcaaaaat ttcaccgaac gtgaccagga tcacagaaat aagcgcgcta
      661 tagggttgcg ggggcagcag gtttaaaacg ataaaagaaa atgacgtcag cagaaggccc
      721 caggaaatat tccgggtgat cttctttttt gcttccagcc tgaatacgag taccatttca
      781 atcaggacaa tgatcattcc gtttaaaccc ataagcaagc cgatatggaa ttctgacagt
      841 tgtaaatgat ccttataata aaccggcaga gtggaaaata gctggaaaaa acacattccg
      901 aagagaatgg ccagactgat aaaaagaata taggttttgt cgtggaaggc tgaccgggca
      961 ggaacaagat catgattggg tctgtgttcg ggttcagcaa ccggttttga tttcagcagg
     1021 taatgcagga gaaatgcagc cgtaatattg gtgagcccgt cgatccagaa taataaatgg
     1081 taattgaaag aagcaataaa tccgcccagt gacccaccga atgcccagcc caggttgatc
     1141 gccaaccggt tcagggaaaa ggaccgggtc ctgttttcct ctttactgta atgcgcgatg
     1201 gcggtattat tcgccgggcg aaatgcttca ttcaccaggc tcagaataaa ggtgaacaga
     1261 cagatcagga gatagtgcct catcaaaccc agaacaataa agagaaggcc tccgccgcaa
     1321 agcgtgacca gctgaatctg gtgaaatccc catttatctg tcagctttcc tccgaggtaa
     1381 cctccgcaaa ttgcgcccag cccaaataaa cccactacga gaccggcatg gaaaagagaa
     1441 aaacccattg ccgtgaggta gatggtcatg aacggaagca ccatggtgcc gctccggttc
     1501 accagcatca ccacggaaag cagccaggtg gaacgggata atccgctgta ggcatttttg
     1561 tataaggtta aggtgctgct gaacgggttg atcattttca tgccgggttg ggaatgcctc
     1621 gatgaaaagc agccggaaac tcaaatatta tataaattgc caccgtgcta aattaggaag
     1681 cttaacagtg attatgaaaa gcgtgcaaag aattttggtc gttttgttcc tgctgctctt
     1741 cggtttcggg atcggcatgc caaaggtccg ggcccaggaa gaggattatt ccaaacatgg
     1801 gtggcagtta atggattacc gggacgatgg attatttgga gccggggtta cccgggccta
     1861 tcgcgaatta ttgaaaggga agaagagtca tttggtcatc gtagcggtta ttgacgacgg
     1921 tatagacacg gcgcacgagg acctggcagg gcatatctgg accaacaaaa aagagattcc
     1981 gggcaatgga aaggatgacg atcataacgg ctatatagat gacgtgcacg gatggaattt
     2041 ccttgggggt aaggatggcc gaaatatcac ggtggaaagt tatgaatcct accgggagta
     2101 ttaccgcctg cggaattccc ggtccgccgg ttcaatggct gcaaaggatc ccgggaatgc
     2161 atattggata aaagtgagaa atggttttct gaaagactcc atccgggagg cgcacacggt
     2221 ttccatggta ggtcagatga ttccgcagat gcggttcacg gacagtttgc tgagagcggt
     2281 actccataaa gactccgtga atgcatggga cctggaagca ctggtgccaa aagactccct
     2341 ggtccggcag gcaaagaaaa gcgggctgaa atatttcagg aggtacggaa tttcatccga
     2401 catgccgctt ggaaaattca tcgtggaggc cgggaaatat ctggaaagcg cgaagcttgt
     2461 cctgaacagc ttttcagaag atccgaatgc catgcgcagc gagatcgtaa aagatgattt
     2521 caacaatatc gacgaccgga attacgggaa taataatatt acggaaggga cctcctcgca
     2581 cggtacgcat gtggccggaa tcattgcggc ctcccggaat aacgggatcg gtattgacgg
     2641 gatagccgat aatgtgcaga tcatgccgat acgcgccgtt ccgcaggggg acgaacgcga
     2701 taaggacgta gccctggcga tccgttatgc ggtggataac ggggcgcgga tcataaacat
     2761 gagttttgga aaatattttt ctcccggcaa gaaatgggtg gatgatgcgg taaaatatgc
     2821 tgaagaacat gatgtgctga tcgtgcacat cgcaggaaat gaatcgctgg atattgatac
     2881 ggtcagcaat tatccgaatc cggacgaacc cggaggtcgc agaaaatcat ctgcttttat
     2941 tacggtgggc gccagtacca ggggcccgga cagtctggtg gtcgcccggt tttccaatta
     3001 tggccggaag gccgttgatc tctatgcgcc cggagtaaag atctattcaa ccgttcccgg
     3061 aaaccagtat gaggcctata gcggaaccag tatggcggca ccggttgtca gcggaatagc
     3121 cgccctgctg ctggaatatt atcctaaact gagtgccggg cagttaaaat atatacttac
     3181 ccattccgta atgaaattat cccatcccgg ggtaagactg gcggcaaccg gtaaaaccgt
     3241 ggattttaaa accttatctg ccagcggagg gatcgtaaat gcctataacg ccctgaaact
     3301 ggcaggctcc ctgaaaggga aaacgggaaa agccttcagg atgaagagga tgcgttgaat
     3361 tattgattgg ggatccccgg tacggtcagg ggcattcgct gaaatccggg cggaggatat
     3421 tgggaaaaaa gacaattttt tgtacattaa taccgttaat tacccgtcac ccttagaaac
     3481 ccaaatctga tccctatgct accccacatc gacaatcaac aggaccgtct tgctgagatc
     3541 tttcgccaga tggacaacca ggttacccag gagagtaaag ttgcatttct taaaattcaa
     3601 agctggcagt ttgccgtccg gacgccggaa ataggcccca cttatctgcg ggaagcagct
     3661 tccatgatca cccagtccct gctgtctttt gttccgaaca gttttgtgtt aagcgaagag
     3721 gggtattacc tgaatccgat cgagaattaa ccggttttca aaagaaagcc atcaggagaa
     3781 gctgacgcat tctttcagtt ccttttccgt ataggccata ttgtattgtt tcaatacatc
     3841 atccggcact ccgttccact gacttggaac attgccaata tatcctacgt cttttacccc
     3901 tatttcagat gtataaaacc cggtagccac caggttgcgc accaggttaa aaaaggccac
     3961 tcccggtctg ttattgggtt ttgctttttt tggccaggca atttcatcca ccatttccat
     4021 ttgctgctcg tgcgtacagt ctttaaagga ctttccaaac cgggtcaggc attgcatatc
     4081 cagccagcgg agtccgcccc gcatcggggt ttggtgttcg ggcatatctt tcacgatgaa
     4141 ctcaatgaat tcaggaactt ttgcctctga ggcgcttccg ctgacttcat ccctgg
//
LOCUS       DASYCU010000210         6658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_772149, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000210 DASYCU010000000
VERSION     DASYCU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772149"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGM24_04260"
     CDS             complement(<1..208)
                     /locus_tag="VGM24_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154609.1"
                     /translation="MKNSSQRKICFLVVLLGLGFFISASGQGTAKPLWVRDFSSLPFP
                     GEMLKNKNPHFTPDSAFYPVSAYDL"
     gene            299..1786
                     /gene="rpoN"
                     /locus_tag="VGM24_04265"
     CDS             299..1786
                     /gene="rpoN"
                     /locus_tag="VGM24_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY4154610.1"
                     /translation="MALSQSLQQKLLQKLSPQQIQLMKLLQVPTANLEERIKEELEEN
                     PALEVAEEDHDDGLTEDSKDEFENEEEDFEPEGTQEEYDNIDISDYVNEHDDEVSDYK
                     LRDDNYPEQDDNKTIAHKVETSFYELLQDQLGMLKLDERRKKIAEQIAGSIDDDGYLR
                     RETASIVDDLAFRQNIESNDQEVEEIIAKIQQFDPPGVAARDLQECLLLQLQRKKAEG
                     KSVELAIAVLSGYFDEFTKKHYDKIQRGLNITEEEFRDVIRQIIKLNPKPGGQVSGTG
                     KGEGYVVPDFFIYHNGGLLELTLNSRNAPDLRISDGYRDMLKDYDRGAKKDKRQKEAV
                     LFIKQKIDAAKWFIDAIKQRQQTLLNTMQTIMEYQHEFFITGDETTLKPMILKDIAER
                     TGLDISTVSRVANSKFVQTEFGTYRLKFFFSESLSTDSGEEVSTREVKKILSNLIEGE
                     SKKKPLSDERLTELLQEKGYNIARRTVAKYREQLNIPVARLRREL"
     gene            1797..2471
                     /locus_tag="VGM24_04270"
     CDS             1797..2471
                     /locus_tag="VGM24_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154611.1"
                     /translation="MESALTNSSVNLNPVQPHPSRIIRSVAHFFSILFHPLLISAYVI
                     AFLIYLHPSVFVGVDAHTRLLRMVSTLLFTVFYPAFVIFIAWRLKLIRKLSLNNMQDR
                     IIGFVVTMFFYWWTWNVFRNLSDIPPVVVHFTLGSFLAICGGWMCNIFFKISMHALAM
                     GGLCAFFTIFSMHDAFASGLYISICILIAGFVCTARLLLAEHTPFEIITGLLVGVLAQ
                     IVAWLF"
     gene            complement(2572..3321)
                     /locus_tag="VGM24_04275"
     CDS             complement(2572..3321)
                     /locus_tag="VGM24_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEY4154612.1"
                     /translation="MRITNRVLTIVLFSLSFLFAPAFEAGNGLRATTGKVSPAGNHRT
                     EKTIEFEAEVSSLYNEANLEESGLKRSVFEYAYKGYLYLLEQHKLSNAHVISICDFTQ
                     SSRSRRLYVVDLETKKILINTYVAHGRRSGGEYAKSFSNNASSHKSSLGFYVTEQTYV
                     GKHGLSLKIHGLEKGFNDKADRRNIVVHGSKYVGSGFLKSNRFNGRSFGCPAVPAGEV
                     DGLIEDIKGGSCLFIYYPEKKYLHSSKILNG"
     gene            3659..4156
                     /locus_tag="VGM24_04280"
     CDS             3659..4156
                     /locus_tag="VGM24_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY4154613.1"
                     /translation="MKTLFLVRHAKSVNHVTAATDFNRSLNDRGVKDAAEMAGRLVKR
                     DIMIDQFVSSPAVRAKTTAEIFISQYNRKPEEILFIPDLYQATSKIFDRVIARFDDQF
                     NHIAIFSHNPGITDYASYLTPTHITHMPTCSVLAVAAAIKSWQNFSVAKKEFLFFETP
                     KGDKD"
     gene            complement(4137..5243)
                     /locus_tag="VGM24_04285"
     CDS             complement(4137..5243)
                     /locus_tag="VGM24_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4154614.1"
                     /translation="MPSQSAVQVLVSPLDWGLGHATRCIPIIHALKNRGVTVLIAASG
                     PQMALLKGEFPALEFLEIPGYGIRYSGKKGGLIRGLLFRIPAILGSIRRENTWLKKLC
                     RQRKIDLIISDNRYGLYHKDKYNVFITHQLFIQSGLGNLADRILLRLNRYFIRRFSEC
                     WIPDFQDPFSLAGVLSHPPHLPSIPVKYAGILSRFIRAEKIFSNPLLILISGPEPQRT
                     EFENRIFEELKNYEGSAVVLRGLPGIPEQKIFSNSRIRIYNHLASGPLNDCLNSSETV
                     ICRSGYSTLMDLARLRKKAILIPTPGQPEQEYLGRYLHEKKWAICVSQDHFNLDSCLK
                     AFSGNAWGFPELPDVPFPEELIAALPDRLNLYRP"
     gene            5470..5718
                     /locus_tag="VGM24_04290"
     CDS             5470..5718
                     /locus_tag="VGM24_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154615.1"
                     /translation="MKTPDRDLLVLMKDEFATEQFMAYELEKLNDLLYRYETLENFCV
                     AHEVFDLNNYKILRGSRYIQRIVRQQEMKPFQFLCNKN"
     gene            complement(5735..>6658)
                     /gene="dnaN"
                     /locus_tag="VGM24_04295"
     CDS             complement(5735..>6658)
                     /gene="dnaN"
                     /locus_tag="VGM24_04295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY4154616.1"
                     /translation="GRVCIPAKILLDSLKNISDQPLAFTIDKQFGVEITSDNGKYKVM
                     GENPDNFPKDPAADETSSFEMPASELTAAINKSLFAVSSDDLRPAMTGVLVEMSKTGI
                     QFVATDAHRLIRFRLKDIECAKSESLIVPKKPLNLLKSALPSTGEQVKLSYNSNHLFV
                     NFGGIQMSCRLIDARFPDYKVVIPAENPYKLVLNRVDFQNALRRVSVFSNKSTNQVVL
                     SIHGSELQLASQDVDFSFEGTERMKCQYDGEDMSIAFNARFLIEMLNATSSEEIRIEL
                     STPTKAGIIKPVEQDGQEDLLMLVMPLMLNS"
BASE COUNT         1797 a         1600 c         1569 g         1692 t
ORIGIN      
        1 gaagatcgta tgctgaaacg ggataaaatg cggaatccgg cgtaaaatgg gggtttttat
       61 tttttaacat ttcccccgga aaaggcagac tgctgaaatc ccttacccat agcggttttg
      121 ccgttccctg cccgctggca gaaataaaaa agcccaaccc caaaaggaca acaagaaaac
      181 aaatttttcg ttgggaacta tttttcataa ttgtatgtaa ttttacggcg cgatttttgt
      241 ggattaggcg gattttttaa aaatttccgc tgtaacaacg actaacccct tataaaagat
      301 ggcattaagt cagtccttac agcaaaagtt actgcaaaaa ctatccccgc aacagattca
      361 gctgatgaaa ctgctgcagg ttcctactgc caacctggaa gaaaggatca aggaagagct
      421 cgaagagaac ccggccctcg aggtggcaga agaagatcat gacgacggac tgaccgaaga
      481 tagcaaggat gaatttgaaa atgaagagga agattttgaa ccggaaggca cccaggagga
      541 atatgataat attgatatca gcgattatgt gaatgagcac gatgatgagg tgtccgatta
      601 taagctccgg gatgataact atccggagca ggacgataac aagacgattg cccacaaggt
      661 cgaaaccagc ttttacgaac tgctccagga ccagctgggc atgctcaaac tggacgagcg
      721 gagaaagaaa attgccgaac agatcgccgg cagcattgat gacgacggct acctgaggcg
      781 tgaaacggct tccattgtgg atgacctggc tttccggcag aacatagaaa gcaatgacca
      841 ggaagtggaa gagatcattg ccaagatcca gcagtttgat ccgccgggtg ttgctgcccg
      901 ggatctccag gaatgcctgc tactgcagtt acaaagaaaa aaggccgaag gaaaaagcgt
      961 ggagcttgcc attgccgtgc tttcgggtta ttttgacgaa ttcaccaaga aacactacga
     1021 taagatccag cggggcctga atattacgga agaggaattc cgtgatgtga tccggcagat
     1081 cataaagctg aacccgaaac ccggcgggca ggtaagcgga acgggcaagg gggaaggtta
     1141 tgttgtgccc gatttcttta tctatcataa tgggggcctg ctcgagctga cccttaattc
     1201 ccggaatgcg cctgacctgc gcatcagtga cggataccgg gacatgctca aagactatga
     1261 tcgcggggcg aaaaaagaca aaaggcaaaa agaagcggtg ctttttatca agcagaaaat
     1321 cgatgccgcc aagtggttca tcgacgccat caaacaaagg cagcaaaccc tgctcaatac
     1381 catgcaaacc attatggaat atcagcatga gtttttcatt accggggatg agacgaccct
     1441 gaagcccatg atcctgaaag atattgccga aagaaccggg ctggatatat caaccgtaag
     1501 ccgggtggcg aacagcaaat ttgtacagac cgaattcggc acctaccggc taaaattctt
     1561 tttcagcgaa tccctgagca ccgacagcgg ggaagaggtt tccacccggg aggtcaagaa
     1621 gatcctcagt aatctcattg aaggggaaag caaaaaaaaa ccactgagcg acgagcggct
     1681 caccgagctt cttcaggaaa agggatataa tattgcacga agaaccgtgg ccaagtaccg
     1741 cgaacagctg aatattcccg tcgcccgcct ccgtagagaa ttataattcg atcaaaatgg
     1801 aatctgcctt aaccaactct tccgttaacc tcaatccggt gcagccgcat ccgtcccgga
     1861 tcatacgctc cgttgcgcat tttttttcga tcctcttcca tccgctgctg atcagcgctt
     1921 acgtaattgc ctttttaatc tacctccacc cgtccgtctt tgtcggcgtg gatgcgcata
     1981 cgcgtttgct gcgcatggtc agcacccttt tgttcaccgt attttatccg gccttcgtca
     2041 tttttatcgc ctggcgcctg aaactgatcc gcaagctgag cctgaacaat atgcaggacc
     2101 ggatcatcgg gtttgtggta accatgttct tttattggtg gacctggaat gtattccgta
     2161 acctgtccga tatcccgccg gtagtggtgc atttcaccct gggatccttt ctggctattt
     2221 gcggcggctg gatgtgcaat atctttttca agatcagcat gcatgctttg gccatgggcg
     2281 gcctctgcgc cttcttcact atcttcagca tgcacgatgc ctttgcttcc ggattgtata
     2341 tttccatatg cattctcatc gccggtttcg tatgcaccgc ccgcctgctg ctggcagagc
     2401 acacgccgtt tgagatcatt accgggcttc tggttggcgt gcttgcgcaa atcgttgcct
     2461 ggttatttta gccgggtgac cgggtcctaa taaaaaaaga aaagcccaat catagggtac
     2521 gaacctatat tgagcctttt caccttcaaa ccatcggaag tttacgtccg cttaaccatt
     2581 tagtattttg gaagagtgca ggtatttttt ctccggataa tagataaaga gacaggaacc
     2641 gccttttatg tcttcaatca gcccatccac ctcaccggcc ggcactgccg ggcaaccaaa
     2701 gcttctgcca ttgaaccggt ttgattttaa aaatcccgaa cccacatatt tggatccgtg
     2761 aacaacgata ttccggcggt cggccttgtc gttaaatcct ttttccaggc catgaatttt
     2821 caagctcagg ccgtgtttac caacatacgt ctgctccgtt acataaaatc caagactgct
     2881 tttatgcgaa ctggcattgt tggaaaatga tttcgcatac tcaccccccg aacggcggcc
     2941 atgggcgaca tacgtattga tcaatatctt tttagtctcc aggtccacca catataagcg
     3001 gcgactccgg gacgactgcg tgaaatcgca gattgaaatg acgtgggcgt tactcagctt
     3061 gtgctgctcc agcaaataga ggtaaccttt ataggcgtat tcaaacacgg agcgtttcag
     3121 gccggattcc tccaggttcg cttcattata taagctgctg acttcggcct caaattcgat
     3181 ggttttttct gtccggtgat tgccggccgg ggatactttt ccggtggtgg cccttaatcc
     3241 gttaccggct tcaaaagcag gagcaaacag gaaggacagg ctgaacagaa cgatggttag
     3301 tacacgattt gttattcgca tagggttttt cggatgttgg ataaacgggt taaagaacga
     3361 aaatattatc tggtaccggt ttttgcaatc tttttgagcc tcatttttta ttgtgttccg
     3421 aataaaaatt ttaaatgcgc aggatctcgt aaaactcctc tgtttttccg ccgcaatccc
     3481 tctgcttttc tgccggaacc ccgaaaaaca acttagtatt tttcctcccc tccaaaaaag
     3541 ggaatgtaaa attactccgt tcagaaaacc cttagtgcct cattaactta aaatttacat
     3601 aattttttag gttccggttc ttcctttaac ttagagaggg agataaaaga aatcaaaaat
     3661 gaaaacctta tttctggtga gacacgccaa aagtgtaaat catgttacgg cggctacgga
     3721 ctttaaccgc tcattaaatg accggggagt taaagatgcc gctgaaatgg cgggaagact
     3781 ggtaaagaga gatattatga ttgaccagtt tgtttccagt cccgccgtgc gggcaaaaac
     3841 cactgccgaa atcttcatca gtcagtataa ccggaaaccg gaagagatcc ttttcattcc
     3901 ggatctgtat caggccactt cgaaaatatt tgatcgcgtg attgcgcgct tcgatgatca
     3961 gttcaatcac atagccattt tttcgcataa tcccggcatt acggattatg caagttatct
     4021 gacgcccacc catatcaccc atatgcccac ctgcagtgtt ttggccgtgg ctgccgccat
     4081 caagagctgg cagaatttct cggttgccaa aaaagaattt cttttttttg aaacccctaa
     4141 gggcgataaa gattaaggcg gtcaggtaat gccgcaatca actcctccgg aaaaggtaca
     4201 tccggcaatt ccggaaaccc ccaggcattt ccggagaagg ctttcaaaca ggaatccaga
     4261 ttaaaatggt cctgcgaaac gcaaatagcc catttttttt catggagata acggcccagg
     4321 tattcctgct ccggctgccc cggcgtagga atgaggattg cttttttacg cagtctcgcc
     4381 agatccatca gcgtgctgta tccgcttctg cagatcaccg tttcggagct gttcaggcaa
     4441 tcattaagcg gtccggaggc aaggtgattg taaatgcgga tccgggaatt cgaaaaaatt
     4501 ttctgctcgg gaatgcccgg caggccccgc agtacgacgg cagacccctc gtaattttta
     4561 agttcttcaa atatcctgtt ttcgaattcc gtccgctgcg gctccgggcc ggagatcagg
     4621 atcagtaacg gattggaaaa gattttttct gcccggatga accgggatag gattccggca
     4681 tatttcaccg gaatggaagg aaggtgcggc ggatgggaca atactccggc aagggaaaac
     4741 ggatcctgaa aatcgggaat ccagcattcg gaaaaccgcc ggataaaata ccggttcagc
     4801 ctaagcagaa tccggtccgc cagatttccc aatcccgact ggataaacaa ctggtgtgtg
     4861 ataaaaacat tgtatttgtc cttatgatat agcccatacc ggttatccga tattataagg
     4921 tcaatttttc tctgccggca gagttttttc agccaggtat tttcccgccg gatactgccc
     4981 aggatagccg gaatcctgaa aagcagaccc cggatcaggc ctcctttttt tcctgaatac
     5041 cggatcccgt agcccggaat ttccaaaaat tcaagcgcgg gaaattcccc cttcagcagg
     5101 gccatttgcg gcccggaggc tgcgatcagg accgttacgc cccgattttt cagcgcatga
     5161 attattggga tacagcgcgt tgcatgtccc agcccccaat ccaacgggga aaccagcacc
     5221 tggacagcgg attgggaggg catgttaatt ttctaaattt gggacaaaaa gctaagatta
     5281 atcacaaaga ttttctaaat tagagcaaat aaatctatga aaaccccgct cgccctattg
     5341 atttttatac tattaatgat tagcattgta ggaacaggct gcagttccag caaccacatc
     5401 ggcggaagca gcaaaaaatg tggctgcggt ttgaacaaag gcatggtcgg ttattaaaat
     5461 ctaatataaa tgaaaacacc ggatagagat ttgctggtcc ttatgaaaga cgaattcgca
     5521 acggagcagt tcatggccta tgaactcgaa aaattaaacg accttcttta ccgttacgag
     5581 actctggaaa acttttgcgt agcacacgaa gttttcgacc tcaacaatta taagatctta
     5641 agaggttccc ggtacattca acgtatcgtg agacaacagg aaatgaaacc ctttcagttt
     5701 ctctgcaaca aaaattaaaa agatttttat cggttcagct gtttagcatg agtggcatca
     5761 ccagcatgag caggtcttcc tgtccatcct gttccaccgg tttaataatg cctgccttgg
     5821 tgggagtgga taactcaatt cgtatttctt ccgatgaagt agcattgagc atttcgatca
     5881 ggaaccgggc attaaacgca atggacatgt cttccccatc gtactggcat ttcattcttt
     5941 cggttccttc aaaggagaaa tcgacatcct gtgaggccag ctggagttcg ctgccgtgaa
     6001 tactcagcac gacctggttg gtgctcttat tgctgaaaac actcacgcgg cggagcgcat
     6061 tctgaaaatc aacccggtta aggacgagtt tatacggatt ctccgccgga atgaccactt
     6121 tataatccgg gaaccgcgca tcgatcaggc ggcaactcat ctggatccct ccgaaattca
     6181 caaataaatg gttactgtta tacgagagtt ttacctgttc gccggtggag ggcagagccg
     6241 actttaacag gttaagcggt ttttttggaa cgatcagaga ttcgcttttt gcgcattcaa
     6301 tatctttcag ccggaaccgg atcagccggt gtgcatcggt ggcaacaaac tggatccccg
     6361 ttttgctcat ctccaccaaa acccccgtca tggcgggcct caggtcatca ctgctgacgg
     6421 caaaaagaga tttattaatg gcggcggtga gttcggaagc cggcatttca aatgaactgg
     6481 tttcatccgc ggcaggatcc ttcggaaaat tatccggatt ttctcccatc accttgtatt
     6541 taccattgtc gctggtaatc tccacgccga attgcttgtc gatggtgaag gccagcggct
     6601 gatcagaaat attcttcagg gaatccagga ggatcttggc cggaatacaa acgcggcc
//
LOCUS       DASYCU010000211         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4105756, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000211 DASYCU010000000
VERSION     DASYCU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4105756"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /gene="fusA"
                     /locus_tag="VGM24_04300"
     CDS             <1..1693
                     /gene="fusA"
                     /locus_tag="VGM24_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY4154617.1"
                     /translation="DFLNVVKQIKEMLGAKAVPLQLPIGAEDSFKGVVDLITMKGIIW
                     DEEGKGMTYQEVPIPEDMVEEVNEWRQHLVEAVAEYDVKLLEKFFEDPNSITQEEMHD
                     AIRKATIDLSIIPMMCGSSFKNKGVQKALDAIVRYLPGPADVAAVLGHDPNSGEEISR
                     KPDAKEPFAALAFKIATDPFVGRLAFFRVYSGHLDSGSYVLNTRTGKNERISRIMQMH
                     ANKQNPIDFIEAGDIGAAVGFKDIKTGDTLCTEDHPIILEKMTFPEPVISVAIEPKTQ
                     ADVDKMGMAIAKLVEEDPTLRVKTDDETGQTILSGMGELHLEIIVDRMRREFKVEINQ
                     GAPQVAYKEAFQKTIEHREILKKQTGGRGKFADIIFELGPADEEFLKEEKGHYQFVND
                     IFGGSIPREFVPAIEKGFESAMTTGVLASYPVDNLKVRVFDGGFHAVDSDSMSFELCA
                     KQGFREAAKKAKPVLLEPIMKVEVITPDQYMGDVTGDLNRRRGMLEGMDSRNNAQVIK
                     AKVPLSEMFGYVTQLRSLSSGRATSTMEFSHYAPAPVNVAEEVIAKVKGKGKVTA"
     gene            1817..>3876
                     /locus_tag="VGM24_04305"
     CDS             1817..>3876
                     /locus_tag="VGM24_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY4154618.1"
                     /translation="MFSNRNYLQKGLFGVILICLSLASPAQLYKTRVWSADGKAYYQA
                     GETGILEYSLPLGVEKVLVSSRELTPAGDSEPLQVRYFFFSADYKKLLIYTNTRKVWR
                     YDTRGDYWVYDLSDLSLKQLGSSLPPSSLLFAKFSPDGKKAAYVSGHNLYVEDLGNHQ
                     IRALTSDGTRKLINGTFDWVYEEEFDCRDGFRWSPDSRQLAFWQINDSSTRDYLMLNT
                     TDSVYSHVVPVEYPVAGQLPSPFKIGVVNVQDDHIRWMDIPTDAILGSYLPRMEWADN
                     SRELIVQHLNRQQNESDIMLCNVSDGSSKNIYSEKDSAWIDIISRWDEDYRMGGWDWL
                     GEGADFIWPSEKDGWRHLYRVSRDGRKQTLITSGNYDVMGISLIDEKENLLYFLASPD
                     NATQQYLYRCRLDGKGRAERVTPANETGTHEYEIAPGGLYAIHRFSNYYTPPSSEWIS
                     LSAHQKLSGSSGIRMPSPADSIASNTSFFRLKTADGVTMDGWMTKPVPFDPSKKYPVL
                     FYVYTEPGSQTVLDKYGETRNPLYQGDLSRDGYIYISIDNRGTPAAKGSAWRNCIYRK
                     IGRLNIHDQAMAAAEILRWPFVDSSRIAVWGWSGGGSATLNLLFQHPDIYKTGIAVAA
                     LGNLLTYDNIYQERYMGIPQETREDYIKGSAISYARYLEGNLLYIHGTGDDNVHFGNA
                     EMLV"
BASE COUNT         1003 a          944 c         1054 g          875 t
ORIGIN      
        1 cgatttcctg aatgtggtaa aacagatcaa ggaaatgctg ggcgcaaaag ccgttcccct
       61 ccaattgccg atcggagcgg aagacagttt caaaggggtg gtggacctga tcaccatgaa
      121 gggaattatc tgggatgaag aagggaaagg aatgacctac caggaagttc ccatcccgga
      181 agatatggtg gaagaagtaa acgaatggcg ccagcacctc gtggaagcgg tagcggaata
      241 tgatgtgaag ctcctggaga aattcttcga agacccgaat tccattaccc aggaggaaat
      301 gcacgatgcc atccgtaagg ctacgatcga tctttccatc attccgatga tgtgcggatc
      361 ttcttttaag aacaaaggcg tgcagaaggc cctggatgcc attgtaaggt atcttccggg
      421 tccggccgac gtggcagccg tgcttgggca cgatccgaac tcgggagaag aaatttcccg
      481 caagccggac gcaaaggagc ctttcgcggc cctggcattt aaaatagcta ccgatccgtt
      541 cgtgggccgc ctggccttct tccgggttta cagcggtcat ctcgattcgg gttcctatgt
      601 attaaacacc cggaccggaa agaacgaacg tatttcccgg atcatgcaga tgcatgccaa
      661 caaacaaaac ccgatcgact ttattgaggc cggcgatatc ggcgcagccg ttggattcaa
      721 ggatattaaa accggggata ccctgtgcac tgaagaccat cccatcattc tggaaaaaat
      781 gactttcccc gagccggtga tctctgtggc catcgagccg aaaacacagg cggatgtgga
      841 taaaatgggt atggccatcg ccaaactcgt ggaggaagac ccgaccctcc gggtaaaaac
      901 ggatgacgaa accggacaga ccatcctgag cggaatggga gaactccacc tggaaattat
      961 tgtggaccgg atgcgtcgtg aatttaaagt ggagatcaac cagggagcgc cccaggtagc
     1021 ctataaagaa gcattccaga aaaccataga acaccgggag atcctgaaaa agcaaacggg
     1081 cggcaggggt aagtttgccg atatcatttt tgaactcgga ccggccgatg aagaattcct
     1141 gaaagaagaa aaaggacact accagtttgt caatgatatt ttcggaggat ccataccccg
     1201 ggagtttgtt ccggctattg aaaagggatt cgaatcagcg atgaccaccg gcgtgttggc
     1261 cagctatccg gtggataatt tgaaagtccg ggtatttgac ggtggattcc acgccgtgga
     1321 ttccgattcg atgtcgtttg agctttgcgc caaacaggga ttccgggagg ctgcaaaaaa
     1381 ggcaaaaccg gtgttgctgg agccgatcat gaaagtagaa gtgatcacgc cggaccagta
     1441 tatgggagat gttacgggag atcttaaccg ccgtcgcggt atgctggaag gtatggatag
     1501 ccggaataat gcgcaggtga tcaaggcaaa agtgccctta tccgaaatgt tcggatatgt
     1561 tacgcagctt cgttcactca gttccggcag agcaacatcg accatggaat tttcccacta
     1621 tgccccggcg cccgtcaatg tggctgaaga agtgatcgcc aaggtaaagg gtaagggcaa
     1681 ggtaactgcc tgaccggttg aatattttct ttttaaaata ataaaagtcc tctttgcacc
     1741 cgcaaaagag gactttttgt taaatggtta aggatcctga agcctaatac ttaattttcg
     1801 gcttcaaatc cacggtatgt tttcaaacag aaattatctt cagaaaggac tgttcggagt
     1861 tatcctgatc tgcctttctc tggccagccc ggcccagttg tataaaacac gggtatggag
     1921 tgcggatggg aaagcctact accaggccgg agagacgggc atcctggaat acagcttgcc
     1981 gctgggggtt gaaaaggtcc tggtcagttc ccgggaactg actccggccg gcgattcaga
     2041 accccttcag gtccgttatt ttttcttttc tgccgattat aaaaaattac tcatctatac
     2101 gaacaccagg aaagtctggc gttacgatac ccggggggat tactgggttt atgatctttc
     2161 ggatctcagc ctgaaacagc tcggcagttc cctgccgccc tcttccctcc tgtttgcgaa
     2221 attttctccg gacgggaaaa aagcggccta tgtcagcggg cataatctgt atgtggaaga
     2281 tctcggcaac caccagatca gggcgcttac ttccgatgga acgcgtaagc tgatcaatgg
     2341 caccttcgat tgggtttatg aagaggaatt tgattgccgg gatggattcc gctggagccc
     2401 ggacagccgg caactggcct tctggcagat caacgacagc agcacccgcg actatctgat
     2461 gctgaatacc accgactccg tttattccca tgtagtcccg gttgaatatc cggttgccgg
     2521 ccagcttccc tcccctttta agatcggggt ggtgaatgtg caggatgatc atatccggtg
     2581 gatggatatt ccgaccgatg ccatcctggg cagctatctg cccaggatgg agtgggcaga
     2641 caacagccgg gaactgatcg ttcagcacct gaaccggcag cagaatgaaa gcgatatcat
     2701 gctctgtaat gtcagcgacg gatcttcaaa aaatatttat tcggaaaaag acagcgcctg
     2761 gatcgatatc atttcccgat gggatgaaga ctaccggatg ggcggctggg actggctcgg
     2821 ggaaggggcc gattttatct ggcccagcga gaaagacggc tggaggcatt tgtaccgggt
     2881 cagccgggac ggcaggaaac agaccctgat cacctccgga aattatgatg tcatgggcat
     2941 ctccctgatc gatgagaaag agaacctgct ttattttctg gcgtccccgg acaatgccac
     3001 ccagcaatac ctttatcgct gccggctcga tggaaaaggc cgggcggaaa gagtaacgcc
     3061 ggctaatgag acgggaaccc atgagtatga aattgccccc ggcggcctgt acgccatcca
     3121 ccggttttca aattattata ctcccccgtc ttccgaatgg ataagccttt cggctcatca
     3181 gaaactttcg ggctcctccg gaatccgtat gcccagtccg gccgacagta tcgcttccaa
     3241 tacctctttt ttcaggctga agacggcaga cggggtgacc atggatggct ggatgacgaa
     3301 acccgttccc ttcgatcctt ccaaaaaata tccggtactt ttctatgttt acaccgagcc
     3361 ggggagccag accgtattgg ataaatacgg agaaaccaga aatcccctgt atcagggaga
     3421 cctgtcaagg gacgggtata tctatatttc cattgataac cggggaactc cggcggccaa
     3481 aggaagcgcc tggagaaatt gcatttaccg gaaaatcggc cggctcaata ttcacgacca
     3541 ggccatggct gccgccgaga tcctccgctg gccttttgtg gacagttcca ggatcgccgt
     3601 ttggggatgg agcggaggcg gttcagcaac cttgaatctg cttttccagc acccggacat
     3661 ttataagacg ggaattgcgg tggccgcact cggcaacctg cttacctatg ataatattta
     3721 tcaggaaagg tatatgggaa ttccgcagga gacccgggaa gattatataa aaggctcagc
     3781 catcagctat gcccgttacc tggagggtaa cctgctttat attcatggga ccggggacga
     3841 caatgtgcat tttggcaatg ccgagatgct ggtgaa
//
LOCUS       DASYCU010000212         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3527873, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000212 DASYCU010000000
VERSION     DASYCU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527873"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VGM24_04310"
     CDS             complement(<1..492)
                     /locus_tag="VGM24_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY4154619.1"
                     /translation="MARAKLPRKSTTVDMTAMCDVAFLLLSFFILATKFKPPEALKVD
                     TPNSVSTKIVLDKNVVQVTIDKAGKVYFSVSESNPSQKAEILNSINTSKNLGLTEAEK
                     KNFINVPNAYIGVPFSQLKGYLDKNPEELKNLNIPGIPVLDSNANEMVDWLRAAQESF
                     QGDK"
     gene            complement(580..1443)
                     /locus_tag="VGM24_04315"
     CDS             complement(580..1443)
                     /locus_tag="VGM24_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY4154620.1"
                     /translation="MAETRPTATVAAKPTTGVQTPKKGNSISWLAPLLCIIIGYLIWR
                     FLIGADSNFTKPDPNGGFWPEQVGAKSGLARMYLGGIIVPILIGALLTVLTFVVERFL
                     TVAKAAGSGNINEFIRKVQYHLANKNVDSAIAECDRQKGSVGNVMKAGLRKYKEMISV
                     GDLDTEQKVLAIQKEVEEATALELPMLEKNLVFLSTIASVATLLGLLGTVIGMIRAFA
                     SLGDSGGGEAAQRLSQGISEALYNTALGIGTSAISIIMYNIFTTKIDGITYGIDESGF
                     TLTQSFASLYK"
     gene            complement(1680..2972)
                     /locus_tag="VGM24_04320"
     CDS             complement(1680..2972)
                     /locus_tag="VGM24_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154621.1"
                     /translation="MRISDVFSLAFRTVRSNKLRTGITVSIIAFGIMALVGINTAIEA
                     MKQKFTESFASMGATGFTIHYKDYQFFGRDNQVTEEKKGAKKEKRSNAKKPITKDEAE
                     MFKRSYHFPAKVSLSIRGGNSNIASLGDKKTNPTVQVAGGDEYYAELNGYTLSGGRSL
                     NELDVQSGRNVCILGNDLVKKFFGDNPERALEKIIKINGIPYRIIGTLESKGASIGRS
                     WDNVAITSYNNVRRFFLNNPISPWNPTPSFNIQVKVGDVNQMDLAIGEAEGVFRPIRR
                     MATTDASNFVIDKSDRFVEELLSNLGKITLSAVIIGFITLTGAAVGLMNIMLVSVTER
                     TKEIGLVKAIGGRQRSIRLQFLYESIIISLMGAVFGIFLGILVGNSFSLVLKTGFVFP
                     WMWLIWGVLICSVVGLLAGLYPSLKASRLNPIQALRYE"
     gene            complement(3035..>3591)
                     /locus_tag="VGM24_04325"
     CDS             complement(3035..>3591)
                     /locus_tag="VGM24_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY4154622.1"
                     /translation="TAVFQQSVKRMLAYSSVAHIGFMLFTLLSLNILAQEGLILYIAT
                     YSAATIGLFAILVKMKDYGFEGFNGMARKHPVLALVATIFLLSLAGIPLTGGFLAKYY
                     MIAAVMRTGHFLWLVILSLVCAAISIYYYFRVIQAMYFKDGDAQVLELTPGFSGWLIV
                     LAIIVLVLGIFPQWLIDRLYIIYI"
BASE COUNT          874 a          937 c          873 g          907 t
ORIGIN      
        1 tttgtctccc tgaaaagatt cctgagccgc ccggagccag tctaccattt cattggcatt
       61 gctgtccagt acgggtattc cgggaatgtt caaattcttc agttcttccg ggttcttgtc
      121 cagatatccc ttcagctgag aaaaaggaac gccgatatag gcattgggaa cattgatgaa
      181 atttttcttt tccgcctctg tcagccccag gttctttgag gtattgatag agttcagaat
      241 ttctgccttc tggctcggat tggattccga aacggaaaaa tagactttcc ccgctttatc
      301 aatggtaacc tgaaccacat tcttgtccag gacaatcttg gtcgaaacgg aattcggcgt
      361 atctaccttc agcgcttccg gcggtttgaa tttggtcgcc aggatgaaga aagataacaa
      421 taggaaggcc acatcacaca tggcggtcat gtctaccgtc gtgctcttcc tgggtaactt
      481 tgctcttgcc atgatgaaag attaatgatt ttactaatta gatcccgttt taccccgttt
      541 tccataaaaa aagggcggct ttttagggcc ggttcccgct tatttgtaga gggaagcaaa
      601 actctgggtc agcgtaaagc cactttcatc gataccgtag gtgatcccgt caatcttggt
      661 agtgaatatg ttatacatga tgatggagat ggctgaagta ccgataccca gggccgtgtt
      721 gtacagggct tcggaaatac cctgtgacag acgctgtgcc gcttcaccac cgccgctgtc
      781 tcccagggag gcaaaggcgc ggatcatccc gattactgtt ccgagcagac ccagcagggt
      841 ggccacggag gcaatggtag acaggaaaac caggttcttt tcaagcatgg gcagttccag
      901 cgcggtcgct tcttccactt ccttctggat ggccagcact ttctgttccg tgtccaggtc
      961 gcctacgctg atcatttcct tgtacttgcg gagacctgcc ttcatcacat tgcccacact
     1021 gcctttctgc cggtcgcatt cggcgatggc tgaatccaca tttttattgg ccagatgata
     1081 ctgcacttta cggataaatt cgttgatgtt tccgctgcct gctgctttgg caactgttaa
     1141 aaaacgttca accacaaaag tcagcacggt cagcaacgca ccaatcagga tcggtacgat
     1201 gattcctccc agatacatac gggcaagacc agattttgca cctacttgtt caggccagaa
     1261 tccgccgttg ggatcgggct ttgtaaaatt gctgtctgct ccaatcagaa acctccagat
     1321 caggtaccca atgataatac aaagcaaggg cgccagccag gaaatagaat tgcccttttt
     1381 tggagtttgc acacccgtag tcggtttcgc agctaccgtt gcagtcggtc ttgtttcagc
     1441 catgatcgtt tgtttttagt gtttatgaaa aatttgaaaa atgatttcgt tttttaacgg
     1501 cctcaaggga taagcaggta aaatttttcg acagggattt gggcttaaac gatctttcgt
     1561 agggaacaca atatagtaaa tttcccgaat gccaaatgga aaatactttt tctttaaaat
     1621 ttattcagtt tctttcaaac ccgtgcctgc attcattctt ccttaacctg cccgcagcat
     1681 cattcgtagc gcagtgcctg tatggggttt agccgggagg ctttgagtga aggatataat
     1741 cccgccagca atccgacaac cgaacagatc agcacacccc agatcagcca catccagggg
     1801 aatacaaatc cggtttttaa tacgagggaa aagctgttgc cgaccaggat gcccaaaaag
     1861 attccaaata ccgcgcccat caggctgatg atgatggact cgtaaagaaa ttgtaaccgg
     1921 atgctgcgtt gcctgccccc gatcgctttg accaggccga tttccttggt gcgttcggtc
     1981 accgatacga gcatgatatt catcaggccg acggccgcgc ccgtgagggt gatgaagccg
     2041 atgatcaccg cactgagtgt aatcttaccc aggttactca gcagctcctc cacaaaacgg
     2101 tcgcttttat cgataacaaa attgctggca tcggtggtgg ccatccgccg gatgggccgg
     2161 aacacccctt ccgcttcccc gatcgccagg tccatctggt ttacatcccc cacctttacc
     2221 tgtatattaa aggagggcgt gggattccag gggctgatcg gattgttgag gaaaaaccgc
     2281 cgcacattat tataggaggt gatggccaca ttgtcccagc tccggccgat gctggcaccc
     2341 ttggactcca acgtcccgat gatccggtag ggaatcccat tgatcttgat gatcttttcc
     2401 aaagcccgtt ccggattgtc tccaaagaat ttcttgacta ggtcattgcc cagaatacaa
     2461 acattccgcc ccgactgcac atccagttcg ttgagggaac gtccgccgct cagagtatag
     2521 ccgttgagct cggcataata ctcatccccg ccggccacct gcaccgtcgg attggttttt
     2581 ttatcgccca gcgaagcaat attgctgttc ccgccccgga tggatagaga tactttggcg
     2641 ggaaaatggt agctgcgctt gaacatttcc gcctcgtcct tggtaatggg ctttttggcg
     2701 ttggaccgct tttccttttt ggcgcccttt ttttcctcgg tcacctggtt gtcccggcca
     2761 aaaaactggt agtctttata atgaatggta aaacccgtgg cgcccatgga agcaaaactt
     2821 tccgtaaact tctgcttcat ggcctcgatg gccgtattga tgcccaccag ggccatgatg
     2881 ccaaaggcaa tgatcgagac cgtaatgccg gtgcgcagct tattgctccg cacggtacgg
     2941 aaagccaggg aaaatacgtc cgaaatcctc ataaatggac ttatcctgtg gctaatttaa
     3001 agattcctga cgacttccgc caccttatct caaatcagat ataaataatg tataagcggt
     3061 caatcagcca ctgggggaag atccccagga ccaggacgat gatggccaga acgatcagcc
     3121 agccgctaaa acccggggtc agttccagca cctgggcatc cccgtccttg aaatacatgg
     3181 cctggatcac ccggaagtaa taatagatgc tgatggccgc gcagaccagg ctgagaatca
     3241 ccagccagag gaagtggccc gtacgcataa cggccgcgat catatagtac ttggccagaa
     3301 acccgcccgt caggggaatt cccgccaggg agagcaggaa aatggtggcc accagggcaa
     3361 gcaccgggtg ctttctggcc atgccgttga atccctcgaa gccatagtcc ttcattttca
     3421 ccaggatggc aaaaagtccg attgtcgcag cgctgtaagt ggctatataa aggatcagcc
     3481 cctcctgggc caggatatta agggaaagca gggtgaagag catgaacccg atatgggcca
     3541 cgctggaata ggccagcatc cgcttcacgc tttgctgaaa aaccgcggta a
//
LOCUS       DASYCU010000213         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1645324, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000213 DASYCU010000000
VERSION     DASYCU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645324"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VGM24_04330"
     CDS             <1..191
                     /locus_tag="VGM24_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY4154623.1"
                     /translation="PGRFISSHLSDWTAEKKQVPIGQGIIDWKEFFAAAKTGGVKNFF
                     VEMDLDTFKDSAAYIHHL"
     gene            complement(208..1047)
                     /locus_tag="VGM24_04335"
     CDS             complement(208..1047)
                     /locus_tag="VGM24_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY4154624.1"
                     /translation="MDIKYFCPRWGSDQIHWDEFLLKVKKAGYDGVEYGIAREVPRAD
                     LQVLFNQARDLDLLIIAQHYDTYEGDFEQHRRKYAEWFEKIKPFRPFKVNSQTGKDFF
                     TLEQNAELIGLAENHAEETGIEVLHETHRNKFAFAAHITKGYLNVIPFLKLTFDVSHW
                     VNVAESYLEDQEEAIALAISRTEHIHARVGYPEGPQVTDPRIPAWQDSVNRHIAWWDR
                     IADRKRKEAPDSLLTITPEFGPFPYMVHSPSTGLPVSSQWDINEYMLNLLKDRYAGNF
                     PSQ"
     gene            complement(1060..1944)
                     /locus_tag="VGM24_04340"
     CDS             complement(1060..1944)
                     /locus_tag="VGM24_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY4154625.1"
                     /translation="MQVKDLSKVHGLINELFQWPSNKKEWDQFRLSEEQVAHFEESGY
                     VSGIKLLDEQQVAVLKNELLELADPKHPGNSLFYEFSMNESTDPAKVLFHALGAWRIS
                     PGFHDILWNPRFLVPAAQLLGNRPIRFWHDQLFCKPAKHGGVVAWHQDYSYWVRSVPM
                     QHLTCWCGLDDADEENGCLYYVPGSHQWGLLEKPALAGDMEGLLPALTEEQKALFKPV
                     PIPLKAGYASFHHPLLIHGSYANTTGRQRRAFVINAFADGTRSNTDDSLLAGTPVFKK
                     GEKLEGDFFPLLFNPSQI"
     gene            2251..3048
                     /locus_tag="VGM24_04345"
     CDS             2251..3048
                     /locus_tag="VGM24_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY4154626.1"
                     /translation="MRPALLLYTLLLSALPFPAGPGDEFCGIRNTSFVAGETIAFKVF
                     YSVAGAYFEGGDALFQVNLEQLNGKAVYHITASGKTNSLLDIGFKVRDKYQTYIDTGT
                     LQPYKFIRDVSEGGYKKYENVSFLQTVGTAISDQGVFKIPKCVQDVLSSIFYARNIDF
                     DKYKPQDKIPFSMFLDNQVYSLYIRYLGKEIIKTRYGKFRAIKFRPLLIKGTIFEGGE
                     KMTVWVSDDANHIPLRAESPITVGKISADMMSYHNLRYRLSSLLSVR"
     gene            3338..>4613
                     /locus_tag="VGM24_04350"
     CDS             3338..>4613
                     /locus_tag="VGM24_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154627.1"
                     /translation="MRTIYKTCKRLIFIALCILFQAKFLSAQVQTARPDVVIDAHCHG
                     FYEYLPQGYSSGSQTYPVIIFLHGSGEVGAGTAATLPTVLRNGPPKLISQGTFPVSFT
                     VNGQSFKFIVISPQFNTWPTQADVLDVVNYTLSHYRVDKTRVYLTGLSMGGGAVWDFA
                     SSTSTPTNTAMLAAIVPISGASSPSSQKATQMVKYLLPVWAFHNQTDPTVTPQNTIGF
                     INDMITARNTAIANKVPVGPAPKMTIFPASGHDAWSEAYDINYRENNMNVYEWMLSYT
                     RGGTSPNAPPVVNAGADKTITLPTSSVALSGTATDAGGSIKSYSWTKISGPSQFTISN
                     AAITNPTISNLVAGTYTFRLSATDNLGATAADDINIIVNSAAANKAPVVSAGADKTIT
                     LPTSSVALSGTATDADGSVKSYNWTKISGPTQF"
BASE COUNT         1229 a         1202 c         1024 g         1158 t
ORIGIN      
        1 atccgggaag gtttatttcc tcccatttat cggattggac tgctgaaaag aaacaggtgc
       61 caatcggcca gggaatcatt gactggaagg aattctttgc agcggccaaa acgggaggcg
      121 tgaagaattt ctttgttgaa atggacttgg atacgtttaa ggacagcgcg gcatatatcc
      181 accatctcta agccggtccc ttaatcttta ttgactggga aaatttccgg cgtatctgtc
      241 tttcagcaga ttcagcatgt attcattgat gtcccactgg ctggaaaccg gcagcccggt
      301 agaaggcgaa tgaaccatat aggggaatgg cccgaattcg ggcgtgatcg taagaagtga
      361 atctggcgct tctttgcgct ttcgatccgc aatccggtcc caccaggcga tatgcctgtt
      421 caccgaatcc tgccacgcgg gaatgcgggg atcggtaacc tgaggtcctt ccggataacc
      481 cacccgcgca tgaatatgtt ccgtgcggga aatggcgagc gctatcgcct cttcctggtc
      541 ctccaggtaa gattccgcca cgtttaccca atgagaaaca tcgaatgtca gcttcaggaa
      601 aggaattacg ttaaggtatc ccttcgttat atgggccgca aaggcaaatt tattccggtg
      661 cgtttcgtga agaacttcaa tcccggtttc ctccgcatga ttttccgcca gtccgatcaa
      721 ctcagcgttc tgctccaggg taaaaaaatc ctttcctgtt tgtgaattca ctttaaatgg
      781 ccggaacggt tttatcttct caaaccattc cgcatatttt ctccggtgct gttcaaaatc
      841 cccttcatac gtatcgtagt gctgggcgat gatcagcaga tccaggtccc tggcctgatt
      901 aaacagaacc tggagatccg cccgcggcac ctcccgggcg atcccatatt caacgccatc
      961 gtaacctgct ttttttactt tcagcagaaa ctcatcccaa tggatttggt cactgcccca
     1021 gcggggacaa aaatatttta tatccatcac ctatgtattt cagatctggc tgggattgaa
     1081 aaggagcggg aaaaaatcac cttccagctt ttcacctttc ttaaaaaccg gcgtgcccgc
     1141 taaaagggaa tcgtctgtat ttgatcgcgt cccatcggcg aatgcattga tgacaaaagc
     1201 gcgacgctgc cgccccgttg tatttgcgta tgatccatga atcaataaag gatggtgaaa
     1261 gctcgcatat cctgctttca gcggtatggg aaccggcttg aataatgctt tttgctcttc
     1321 agtcaacgcg ggaagaaggc cttccatatc tcccgccagc gcaggttttt ccagcaaacc
     1381 ccactggtgg cttccgggca cataatacaa acagccattt tcctcatctg catcatcgag
     1441 tccgcaccaa caggtcagat gctgcatggg aacactccgg acccagtagg aataatcctg
     1501 gtgccaggct accacaccgc cgtgcttcgc cggtttgcaa aacaactggt cgtgccagaa
     1561 tcgtatcggc cggttaccta agagctgggc cgccggcact aaaaaccggg gattccataa
     1621 tatgtcatga aagcccggag atatgcgcca ggcacccaaa gcatggaaaa gcaccttagc
     1681 cggatcggtg gattcattca ttgaaaattc gtaaaacaga gaattgcccg gatgcttcgg
     1741 atcagccagt tccagcaatt catttttcaa tacggcgacc tgttgttcat ccagcagttt
     1801 aattccggat acataaccgg attcctcaaa atgcgctacc tgttcttcac taagcctgaa
     1861 ctgatcccat tcttttttat ttgacggcca ttgaaacagt tcgtttatca aaccatgtac
     1921 tttactgagg tccttcactt gcatgatgca tcattttatt ggtaagttaa cgttatctgc
     1981 gcaggcattc tttctttcat gcaaaaatac ggccggtcgc ttttccaggc acggcagatc
     2041 cggtccacta tcttaacccg gcaaagtgcc ggcccggtga attcatggcc tgaagatagc
     2101 cccctctcct gatcagttca aattttctgt tgcattccga accgatctgc ataccgaagc
     2161 caatccctca tcgcagggga gaatattttc gataccagaa tgatcctgcc gggacttcac
     2221 taattttaaa tataaaattt ccgaaaacag atgagaccgg ccctcctcct ttacacctta
     2281 ctgttgtcag cccttccctt tccggccggc ccgggagatg aattctgcgg aatcagaaat
     2341 accagcttcg ttgcgggaga aaccattgca tttaaagtct tttattccgt ggcaggggct
     2401 tatttcgaag gcggcgatgc gctgttccag gtaaacctgg aacagctgaa tggaaaggca
     2461 gtataccata tcacggcaag cggcaaaacg aatagtcttc tcgatatcgg atttaaggtc
     2521 cgggacaagt accagactta tattgatacc ggaacacttc agccatacaa gtttattcgg
     2581 gacgttagtg aaggcggata taaaaaatat gaaaacgtaa gctttctcca gaccgtcgga
     2641 acggccatca gcgaccaggg tgttttcaaa atacctaaat gcgtccagga cgtgctgagc
     2701 tccattttct atgcaaggaa tatcgatttc gacaaatata agccacagga taaaattccg
     2761 ttttccatgt tcctggataa ccaggtatat tccctgtata tccgttatct cggtaaagag
     2821 atcatcaaaa cgaggtatgg aaaattcagg gcaatcaaat ttagacccct cctgatcaaa
     2881 gggacaatat ttgaaggggg agaaaagatg acggtttggg tcagcgatga tgccaatcac
     2941 ataccgctaa gagccgaaag ccccatcacc gtgggaaaga taagcgctga catgatgagt
     3001 tatcacaacc tcagatatcg cttgagctct ttgttaagcg tccgttaatg catccggact
     3061 ccgctgaagc gaccgtaaaa aaaactcctc ctgcaatttc ctaaaaaaag aatttttatt
     3121 aagggaaccc ttatgcgacg ttcaaatatc agggtttaca ctgaccggat attaattgtt
     3181 cgtttaacaa ttaataatca gtaaacacct taaaaaaaaa ttataaaaaa ttttcccaat
     3241 taacaaaata tggaacaatt tttggctgct caaggaagat tctaaatctc ccccctaaaa
     3301 tccgaccgaa atattttata aaacaaaata gagtcctatg agaactatct acaagacctg
     3361 taagaggctt atttttatcg ctttatgcat tctatttcaa gctaaatttt tatcagcgca
     3421 ggttcagacg gcaagacccg atgtggtcat agatgcccat tgtcatggtt tctacgaata
     3481 cctgccgcag ggttattcat cgggtagtca gacctatccc gtaattattt tcctccatgg
     3541 atccggagaa gtgggcgccg gtacggcggc caccttacca accgttcttc gcaacggtcc
     3601 tcccaaactg atcagtcagg gaaccttccc ggtatccttc acggttaatg gccagagttt
     3661 taaattcatt gtaatctccc ctcagttcaa tacctggcca acgcaggcgg atgtgctgga
     3721 tgtggtcaat tatacattat ctcattaccg ggttgacaag acccgggttt atctgacggg
     3781 cctcagcatg ggaggcggcg ccgtttggga ttttgccagt tccaccagca caccgaccaa
     3841 caccgcgatg cttgccgcta tcgtaccgat cagcggtgcg tcctccccaa gctcacaaaa
     3901 ggccacccag atggtcaaat atctgttgcc ggtttgggca ttccacaacc agaccgaccc
     3961 aacagtaact ccccaaaata ccatcggttt tattaacgat atgatcactg cgcggaatac
     4021 ggccatcgcc aataaagtac ccgtaggccc cgcacctaaa atgaccatct ttcccgccag
     4081 cggtcatgat gcctggagcg aggcctacga tatcaattac cgcgagaata atatgaatgt
     4141 atacgagtgg atgctttcgt atacccgggg aggcacttct ccaaatgctc cgccggtggt
     4201 gaatgccggg gcagacaaaa ccattaccct gcctaccagc agcgttgccc tttccggtac
     4261 cgccaccgat gccggcggaa gtattaaatc ctattcctgg accaagatca gcggaccttc
     4321 ccaattcacc atcagcaatg ccgcgataac gaacccgacc atcagcaatc ttgttgccgg
     4381 tacctatact ttccgtttgt cggccaccga taatctcgga gccaccgcgg cggacgacat
     4441 taatataatt gtaaacagcg cagcagccaa taaagcgccg gtagtcagtg ccggggcaga
     4501 caagaccatt accctgccta ccagcagcgt tgccctttcc ggcaccgcca ccgacgcgga
     4561 cggcagtgta aaatcgtata actggaccaa gatcagcgga cccacgcaat tca
//
LOCUS       DASYCU010000214         6514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_193594, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000214 DASYCU010000000
VERSION     DASYCU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193594"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2162)
                     /gene="gyrA"
                     /locus_tag="VGM24_04355"
     CDS             complement(<1..2162)
                     /gene="gyrA"
                     /locus_tag="VGM24_04355"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY4154628.1"
                     /translation="MEDQIIPEETQDNNRAIPINIEEQMKTAYIDYSMSVIVSRALPD
                     VRDGLKPVHRRILFGMNELGNNSNKPYKKAARIVGEVMGKYHPHGDAAIYHTMVRMAQ
                     EWNIRYVLVDRQGNFGSQDGDGPAAMRYTEARLHRLAESMLEDIEKETVDFQPNFDDS
                     LTEPVVLPSRIPQLLINGASGIAVGMATNILPHNLSEVIDGCVAYIDNRDISIAEVMQ
                     YVKAPDFPTGGVLYGMEGVKLAMHTGRGRAVLRGKLQVDTKPSGREQIIITEVPYQVN
                     RDGLCDRIGQLVNEKTIEGITHVNNESNEKEGTRIVIDLRRDAVANVVVNQLFKHTEL
                     QTSYGINNVALVKGRPKTLNVLEMITEFVEFRHEVVIRRTQFELREAEKRAHILQGYL
                     IALDHLDEVISLIRSSATPDLAKDNLVNAGWGLSEIQAKAILELRLQRLTGMERDKIR
                     NEFDELMKQIAFLQELLQDEGKRFGVIRKELLEIKEKFGDARKTEITYLDDEMQIKDL
                     IKEEDVVITISHHGYIKRTSATEYRQQRRGGRGASGGKTRLEDYIEHLFVASTHHTLL
                     FFTEKGRCYWLNVYQIPEGDKVSKGRAVQNLIQIPQDDKIKAIIDVKDIKDSAFLNSH
                     FIVLCTKQGVIKKTMLEDFSRPRQNGVNAITILENDHLLDARLTDGNCEIMMAVKSGR
                     AIRFPEEKVRPTGRGAIGVAGIEVDDSSDEVIGMICVN"
     gene            complement(2259..2753)
                     /locus_tag="VGM24_04360"
     CDS             complement(2259..2753)
                     /locus_tag="VGM24_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY4154629.1"
                     /translation="MDKNYILSPEVAEKKMKRMAYEILENNFGEQEFILAGIRDKGLV
                     IARNIQALLNGISKISTELLSVNLDKNNPQVVTLDKQISLRDKVILLIDDVSNSGRTL
                     LYALKPFLNTDLRKVQTLVLVERSHNAFPVKPDYVGLSVATTLQEHIVVEVEGDLVKG
                     AWLE"
     gene            2826..4130
                     /locus_tag="VGM24_04365"
     CDS             2826..4130
                     /locus_tag="VGM24_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY4154630.1"
                     /translation="MLQQIAFILLAGSAILLFSIKARRIRMNILLGMDEDYSGHRLLR
                     WKNLLLLAFGQKKMFRNPLVAVLHFFIYAGFIIINLEVLEIFLDGLFGTHRMFAAPLG
                     ASYKLLINAFEFLALAVLIACVVFLCRRNILKIRRFAKDLDGWPRSDANYILLTEIVL
                     MALFLTLNASDTLLQERLYGHYANRLTGNFAFSQWLHPLLNGFSDKQLAGIERGCWWL
                     HIAGIFAFLNYLPYSKHLHILLAFPNAYYARLYPEGKIRNMKSVQQEVLYAMQPDQAP
                     AGAAPPEKFGARDVFDLSWRNLLDAYSCTECGRCTAACPANLTGKKLSPRKIMMDTRD
                     RLEEVGKNVRLNGTFKDDGKKLLQDYISEEELRACTSCNACVEECPVSISPLEIIFEL
                     RRSLVMEESKAPPEWNSMFSNVENNFAPWKFSPDDRDAWTNG"
     gene            complement(4127..4753)
                     /locus_tag="VGM24_04370"
     CDS             complement(4127..4753)
                     /locus_tag="VGM24_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY4154631.1"
                     /translation="MHLLFTILLGFGLSFLGQLPLGTMSITATQISVQENFGNAWKYT
                     VGVSLIEMAYLRLVLSGVQWIMQHKLFFSILNWITLVFFLGLGVWSFISAQKQDAGKK
                     ALLLNNRINRFMLGVSMSALNPAQIPFWFIWSGYFLDSGWLSSGSVSFNWFTLGCGLG
                     TVAGLALYMYGGNFLVTKMHTSNKTLNKIMGVVFIIAAIAQFYRLMKP"
     gene            complement(4760..5830)
                     /locus_tag="VGM24_04375"
     CDS             complement(4760..5830)
                     /locus_tag="VGM24_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154632.1"
                     /translation="MNRFRGMEGRIFWIRLLHWEYWHFHTVYLPIYPIWLFLCLRARS
                     LFFFSASNPRIKNGGFLSESKQEIYELVPPELQPRTLFFETAADPQKVLDRLLAGQFR
                     FPLIGKPNVGGRGRGVRKLENEADVLFYAGQATVDFHIQEFVSFEKEMGIFYYRFPDQ
                     PEGKISGMVGKEFLTVTGDGKQSVRDLLLLNPRGLLQLPELEKNRPELLKEVVPEGTV
                     FTVVPYGNHARGAKFVDHSDQLSPGLCRWLDSLCRQIPDFYFGRLDIRYRDRQLLEAG
                     KDFVILEVNGAGSEPTHIYDPGHSLFFAWKEIVRHWIILFRISRANHRRGYPYLSFSE
                     GLRMFREDKFNSGKLDLMKKLH"
     gene            complement(5827..>6514)
                     /locus_tag="VGM24_04380"
     CDS             complement(5827..>6514)
                     /locus_tag="VGM24_04380"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY4154633.1"
                     /translation="TASRDEQRERTPALAPEIYRQNGKKLLYPKDTVAGGTWIAIQEN
                     GNIMVLLNGALEKHVRQPPYRKSRGLILLDLVSSDSSVTAFNRAGLDQIEPFTAILVE
                     GGNLFSCQWNGSLRSVKKLNSGSPHIWSSVTLYDPAATEKREDWFRAWLTGNPIPSSQ
                     DILRFHQETGDGDPRNDLLMNRSDNVFTHSISSVFLAGRLARFHYLDLRAMKTASRTI
                     RFSKAIPVNA"
BASE COUNT         1574 a         1748 c         1528 g         1664 t
ORIGIN      
        1 ttattgacac atatcatgcc aatcacttcg tctgaactat catcaacttc tatacccgca
       61 acaccaatgg cgccccgtcc ggtagggcgg actttctcct ccgggaaccg gatggcgcgg
      121 cccgatttga ccgccatcat gatctcacag ttaccgtccg tcagccgggc gtccagcagg
      181 tgatcatttt cgagaatagt tatggcgttc accccattct gacgggggcg gctgaaatct
      241 tccagcatgg tctttttaat cacaccctgt ttggtgcaca gcacgataaa atgactattc
      301 agaaatgcac tgtccttaat atcttttacg tcgatgatcg cttttatctt atcatcctgc
      361 ggaatctgaa tcaggttctg taccgcccgg cccttgctta ccttatctcc ctcggggatc
      421 tgataaacat tcaaccagta acaccttccc ttctcggtaa agaacagcag ggtatgatga
      481 gtggaagcta caaacaaatg ttctatataa tcctccagcc gggtttttcc tccactggcc
      541 cccctgcctc cgcgccgttg ctggcggtat tccgtagccg aagtacgttt aatatatccg
      601 tgatgggaaa tcgtgatcac cacatcttcc tctttgatca gatccttgat ctgcatctcg
      661 tcatcaagat aagtgatttc cgttttacgc gcatcaccaa atttttcctt tatctccagc
      721 aactctttcc ggatcactcc aaaacgtttt ccctcatcct gcaataattc ctggagaaag
      781 gcgatttgct tcatcagctc gtcaaactca ttccggatct tatcccgctc catgccggtt
      841 aagcgttgca gacgcaactc caggatggct ttggcctgga tctcactgag cccccagcct
      901 gcattcacaa ggttatcctt agcgaggtcc ggtgttgcgg aggatctgat cagggaaatc
      961 acttcatcca gatgatccag ggcaatcaga tatccctgaa gaatatgagc ccgtttttcc
     1021 gcttcccgga gttcgaactg cgtgcgccgg atcaccactt cgtgacggaa ttctacgaat
     1081 tcggtgatca tttccagcac atttaaggtt tttggccggc ccttgaccag ggcaacattg
     1141 ttgatcccgt aagaagtctg gagttctgta tgtttaaata actggttgac gaccacattg
     1201 gccacggcat cccggcgcaa atcaatgacg atccgggtgc cctctttttc attcgactca
     1261 ttgttcacgt gcgtaatgcc ttcaatcgtt ttttcattta ccagctgacc tatccggtcg
     1321 cagagaccgt cccggttaac ctgataaggc acttcggtga taatgatctg ttcccttccg
     1381 ctcggcttgg tgtctacctg aagtttaccg cgaagcaccg cccggccgcg gccggtatgc
     1441 atggccagct tcaccccttc cattccataa aggactccac cggttggaaa atcgggggcc
     1501 ttcacatatt gcatgacttc cgctatcgaa atgtcccggt tgtcgatata ggcaacacaa
     1561 ccatctatca cttcagaaag attgtgaggt agaatattgg tggccatccc tacggcaatt
     1621 ccgctggccc cattgatcag taactggggg atccgggaag gaagcactac cggttcggta
     1681 agggaatcat caaaattcgg ctggaaatcc accgtctctt tttcgatatc ctccagcata
     1741 ctttctgcca gccggtgcag tctggcttcg gtataccgca tggctgcggg accgtcccca
     1801 tcctggctgc cgaaatttcc ctgacggtcc accagcacat agcggatatt ccactcctgc
     1861 gccatacgca ccatggtatg atagatcgct gcatctccgt gcggatggta tttacccatc
     1921 acctcaccca caatacgggc agcttttttg tacggcttgt tgctgttatt gcccagctca
     1981 ttcatcccga acaggatgcg gcgatgaacc ggcttcaacc catcccgcac atcgggaagt
     2041 gcccggctta cgatgaccga catagaataa tctatgtagg ccgtcttcat ctgctcttca
     2101 atgtttatgg gaatggcgcg gttattgtcc tgcgtttcct ctggtataat ttgatcttcc
     2161 ataattgatt aaatccctga attttctctg cttttacaag ggcacaaagg taatgaaatc
     2221 agcccgcaat ggcctataac aaagggccta ttttcggctt attccagcca ggcccctttt
     2281 accaggtccc cctccacttc aacaacaata tgctcctgga gggtagtcgc aacagataag
     2341 ccgacataat cgggtttcac cggaaaagca ttgtggctgc gttccaccag caccagggtc
     2401 tggacctttc tgagatccgt gtttaagaag ggttttaatg catacaagag ggtcctgccg
     2461 ctgttcgaca catcgtcgat taacaggata accttgtccc gcaaggaaat ctgtttatcc
     2521 aatgtaacca cctgcggatt atttttatcc aggttaacac ttagcaattc ggtggatatt
     2581 ttcgagatcc cgttcagcag cgcctggata ttccgggcga tcaccagtcc cttgtcccgg
     2641 atgccggcca ggataaattc ctgttcgcca aaattattct ccaggatttc ataggccatc
     2701 cttttcattt ttttttcggc cacttccggg gaaagtatat aatttttatc catgccccaa
     2761 ggtaaagaaa atcacaaatc ctgcaaatct gatctatctt caggttgtca taaatccatg
     2821 agctgatgct tcaacagatc gcctttattt tacttgccgg atcagccatc ctgttgtttt
     2881 ccataaaggc ccgccgcatc cggatgaata tccttttggg aatggacgag gattattccg
     2941 gccaccggct tcttcgctgg aaaaacctgc tcctgctcgc tttcggccag aaaaaaatgt
     3001 tccggaaccc tctggtggcg gtcctgcatt ttttcattta cgccggattt atcattatca
     3061 atctggaggt gctggaaatt tttctggacg gactgtttgg cactcaccgg atgtttgccg
     3121 caccgctcgg cgcatcctat aaactgctga tcaatgcgtt tgagtttctc gcgctggctg
     3181 ttctgatcgc ctgtgtcgtt tttttatgca ggagaaatat tctcaaaatc cggcggtttg
     3241 caaaggacct ggacggatgg ccaagatcgg atgccaatta tatcctgcta acggaaattg
     3301 tcctgatggc cctcttttta acgcttaacg catcggatac tttattacag gaaagattgt
     3361 acggccacta tgcaaaccgg ctcaccggaa attttgcttt ttcacaatgg cttcaccccc
     3421 tgctcaacgg tttttcagat aaacagctgg ccggcattga acgcggttgc tggtggctgc
     3481 atatcgccgg catttttgcc tttctcaact atctcccgta ttccaaacac ctgcatattc
     3541 tgctggcatt cccgaatgcc tattatgccc gtttatatcc cgaaggaaag atccggaaca
     3601 tgaaatccgt tcagcaggag gtcttgtatg cgatgcaacc cgatcaggca cccgccggag
     3661 cggctcctcc cgagaaattc ggcgcccggg acgtattcga cctgagctgg cgcaacctgc
     3721 tggacgccta ttcctgcacg gaatgcggcc gatgcaccgc tgcctgcccg gccaacctga
     3781 ccgggaagaa attatctccc cggaaaatta tgatggatac ccgggaccgc ctggaagaag
     3841 ttggtaaaaa tgtgcgcctc aacggaacgt ttaaagacga tggaaaaaaa ctgttgcagg
     3901 attacatcag tgaagaagaa ttacgggcct gcaccagctg caatgcctgc gtggaagagt
     3961 gcccggtaag catcagcccc ctggaaatta tttttgaatt gcggcgctcc ctcgtaatgg
     4021 aagaaagcaa ggccccgccg gaatggaaca gcatgttcag caacgtggaa aataatttcg
     4081 ctccctggaa gtttagcccg gatgaccgcg atgcctggac aaacggttag ggcttcatca
     4141 accggtagaa ttgtgcgatg gccgcaatga tgaagacgac gcccattatt ttattcagtg
     4201 ttttattgct ggtgtgcatt ttagtaacca gaaaatttcc cccatacata tagagtgcca
     4261 gtccggccac cgttcccagg ccgcagccca gggtgaacca gttgaatgaa acggatccgg
     4321 agctcagcca tccgctatcc aaaaaatagc ctgaccagat aaaccaaaag gggatctggg
     4381 ccggattaag tgcactcatg ctgacgccca gcataaaccg gttgatccgg ttgttcagca
     4441 gcagggcctt ttttccggca tcctgttttt gggcggagat aaagctccat acccccagcc
     4501 ccaggaaaaa gaccagggtt atccagttga gaatgctgaa aaataattta tgctgcatga
     4561 tccactggac gccggacaaa accaggcgca ggtaggccat ttcaatcagc gatacgccca
     4621 ccgtatattt ccaggcgttg ccaaaatttt cctgcacgga aatctgggtg gctgtgatgc
     4681 tcatggttcc cagaggcagc tggcccaaaa aactaaggcc aaatccgagc aggatggtaa
     4741 ataacaaatg catgaaaccc taatgaagtt ttttcatcag atccaatttt ccggaattga
     4801 acttatcttc cctgaacatg cgtagccctt cagaaaagga aagataagga taaccgcggc
     4861 gatgattggc gcggcttatc cggaataata tgatccagtg cctgacaatt tccttccagg
     4921 caaaaaatag agaatggccg ggatcatata tgtgggtggg ctcgctgcct gcgccattca
     4981 cttcgagaat gacgaaatcc ttcccggctt ccagcaattg ccggtcacgg taacggatat
     5041 ccaggcggcc aaaatagaaa tccggaatct gccggcacag ggaatccagc caccggcaaa
     5101 ggccaggact gagctgatcc gagtggtcta caaattttgc tccccgcgca tggttaccat
     5161 agggaaccac ggtaaaaacc gtgccttccg gaactacttc ttttaacaat tccgggcggt
     5221 ttttttccag ttcgggcaat tgaaggaggc ctcggggatt aagcagcagc aggtcacgga
     5281 cggattgttt accgtctccc gtcacggtca aaaattcttt cccaaccatt ccggaaatct
     5341 tcccctcggg ctgatccggg aaccggtaat aaaaaatacc catctccttt tcgaaggaaa
     5401 caaattcctg aatatgaaaa tccaccgtag cctgaccggc ataaaataaa acatccgcct
     5461 cgttctccag cttccttacg ccccttcccc tgccgcccac attgggcttg ccgatcaggg
     5521 gaaaccggaa ttgtccggca aggagccggt ccaatacttt ttgaggatcg gcggcggttt
     5581 caaaaaataa tgttcttggc tgaagctccg gaggaaccag ttcataaatt tcctgtttgg
     5641 attccgagag aaagccgcca ttttttatcc gggggttgga cgcggagaaa aaaaacaggg
     5701 aacgggcccg caaacataaa aaaagccaga tcggatagat gggcagataa acggtgtgga
     5761 aatgccagta ttcccagtgt aacagccgga tccaaaagat ccggccttcc atgccccgga
     5821 accggttcat gcgtttaccg ggattgcttt tgaaaagcgg atggtccggc tggccgtctt
     5881 catcgcgcgt aaatccaggt aatgaaacct ggccaggcgg ccggcaagaa aaacagaact
     5941 gatactgtgg gtaaatacgt tatcgctcct gttcatcaaa agatcatttc gtggatcacc
     6001 gtctcccgtc tcctggtgaa aacgcagaat atcctgtgaa gaaggaattg ggttgccggt
     6061 taaccaggcc ctgaaccagt cctctctctt ttcggttgcg gccgggtcat ataaggttac
     6121 ggaagaccag atatgaggtg atccggaatt cagtttcttt actgaccgga gggacccgtt
     6181 ccattggcag gaaaataagt taccgccctc caccaggatg gcggtaaacg gttcgatctg
     6241 atccagaccg gcccggttaa atgccgttac ggaagaatcc gaagatacca gatccagcag
     6301 gatcaggccc ctgcttttgc gatagggcgg ctgacggaca tgcttttcca gcgctccgtt
     6361 caggagcacc ataatgtttc cgttttcctg gatggcgatc caggtacctc cggccaccgt
     6421 atctttcgga tataataatt ttttcccgtt ctgccggtat atctccggtg ccagggcagg
     6481 cgtcctttcc cgctgctcat cgcggctggc cgtg
//
LOCUS       DASYCU010000215         4048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2947952, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000215 DASYCU010000000
VERSION     DASYCU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947952"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGM24_04385"
     CDS             complement(<1..398)
                     /locus_tag="VGM24_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154634.1"
                     /translation="MKNLLLVLFLIPVSFYPQAQDIRGSWQGTLEAGAQKLKVIFHIK
                     SDSLSGYRSDFDSPDQGALGIPCSETRVKADSVEMLIKVINGGYRGKWDGKNSISGYY
                     FQNGARFPLDLSRAPDKPAALVRPQEPKPP"
     gene            511..1509
                     /locus_tag="VGM24_04390"
     CDS             511..1509
                     /locus_tag="VGM24_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEY4154635.1"
                     /translation="MLLKSTNIMNKGPVSQFVAHHYRHFNAAALVDAAKGYETHLTEG
                     GKMMVTLAGAMSTAELGVSLAEMIRQDKIHIISCTGANLEEDIMNLVAHSHYKRVPHY
                     RDLTPQQEWELLEKGLNRVTDTCIPEEEAFRKIQQHIVRLWKDADQKGERYFPHEFMY
                     KLLLSRVMEEGYEIDVRDSWMIAAAEKNLPIVVGGWEDSTMGNIFASYIVKGEMKPTT
                     MKSGIEYMVWLTDWYRKNSGGKGIGFFQVGGGIAGDFPICVVPMMYQDLEWHDVPFWS
                     YFCQVSDSTTSYGSYSGAVPNEKITWGKLDIHTPKYIVESDATIVVPLIFAWILGW"
     gene            1579..3621
                     /gene="paaZ"
                     /locus_tag="VGM24_04395"
     CDS             1579..3621
                     /gene="paaZ"
                     /locus_tag="VGM24_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEY4154636.1"
                     /translation="MKKLGNYIAGKWIEGEGEGQTLFDAVTGQAICQAGTGGLDFQQM
                     LDYARNTGNPALRKMNFHERGRMLKDLALHLQKQVELFYRISYQTGATRADSWIDIEG
                     GIGNLFSYAALRRKFPDQPFCTDGDPVNLSKNNSFMGHHLLVPKEGVAVHINAYNFPV
                     WGMLEKIAVNFLAGVPAIVKPATVTSYLTEAVVREIHASGILPPGALQLICGSTGNLL
                     DLLTAQDTVTFTGSASTGLKLKSNPNILRENVSFTMEADSLNCIVLGEDADPSKPEWD
                     IFVKEVRKEMTVKAGQKCTAIRRIFVPENKLEEMGKSIFKSLAQTVIGNPSNEKVRMG
                     SLAGREQRREVREQVKKLLNSSRIIYGSVNEVELLDADPDKGAFMSPLLLLNEQPFSG
                     EEAHCIEAFGPVSTLMPYKNMDEAIALSKKGKGSLCSSIVTADDRIAKQYVTRAATHH
                     GRILILNRDCARESTGHGSPLPMLVHGGPGRAGGGEEMGGVRGVKHYMQRVAIQGSPT
                     TLTAITSVYQPHARQKLEEKHPFRKYFEELQIGDTALTHKRTITESDISQFANLSWDH
                     FYPHTDFTSLEGTIFEKPVAHGYFILSAAAGLFVDAAKGPVLLNYGIEECRFVKPVYA
                     GTTIGVRLTCQEKTGQELKEGDAVPKGIVKWFVDVYDDTGESVMIASILTMVRKKS"
     gene            3627..>4048
                     /locus_tag="VGM24_04400"
     CDS             3627..>4048
                     /locus_tag="VGM24_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4154637.1"
                     /translation="MNGNPSGYVRSERHGASMTIEFFHPQSNSLPGALLGELATQIHS
                     AGLDEEIRVILLRSVGEKAFCAGASFAELSRIRTAREGEDFFNGFAHVLNSMRKCPKF
                     IIARVQGKCVGGGAGLVAAADYAIALTGAEIKLSELAIG"
BASE COUNT         1051 a          950 c         1117 g          930 t
ORIGIN      
        1 aagggtggtt ttggttcctg cggacggacc agggcagcgg gtttatccgg tgcgcgggaa
       61 agatccaggg ggaaccgggc tccattctga aaataatatc cggagatgct gttctttcca
      121 tcccattttc cccggtaacc gccattgatc accttaatca gcatttccac gctgtccgct
      181 tttacccttg tttcgctgca gggaattccg agcgcgccct ggtccgggct gtcaaaatca
      241 gaccggtagc ctgaaaggga gtctgacttg atatggaaaa tgaccttaag tttttgagcg
      301 cccgcttcca gggttccctg ccagcttccc ctgatatcct gtgcttgcgg gtaaaaagaa
      361 accgggatca ggaacaggac cagtaagaga tttttcacga cagaaggttt tactaaaatt
      421 acatttaaaa aaattctccg gaaaaatcat tgaaagccat ctgccgtgtc cccgggattg
      481 ttttgaaggg ggaactaaaa aaaattattt ttgctactca agagtaccaa catcatgaat
      541 aaaggaccgg tttcccaatt tgtagcgcat cattacagac attttaatgc agcagccctg
      601 gtggatgcgg cgaaggggta tgaaacccat ttaacggaag gcggcaaaat gatggtcacc
      661 ctggccggcg ccatgagtac tgcggaactg ggggtttcgc ttgccgaaat gatccggcag
      721 gataaaattc acatcatttc ctgcaccggc gccaacctcg aagaagacat catgaacctg
      781 gtagcccatt cccactacaa gcgggttccg cattaccgcg atcttacccc gcaacaggaa
      841 tgggagctgc tggaaaaagg attgaaccgg gtgacggata cctgcatccc ggaagaagaa
      901 gctttccgga agatccagca gcatatcgtg cggctttgga aagacgcaga tcagaaaggc
      961 gagcggtatt ttccgcatga gttcatgtac aagctgctgc tgagcagggt gatggaggaa
     1021 gggtacgaaa tcgatgtgag ggatagctgg atgatcgcgg cggcggaaaa aaatcttccg
     1081 atcgtggtgg gcggatggga agacagcacc atgggtaata tttttgcttc ctatatcgta
     1141 aagggtgaaa tgaagcccac caccatgaag agcgggattg aatacatggt gtggctgact
     1201 gactggtacc ggaaaaattc cggaggaaag ggcataggat tttttcaggt cggcggcggc
     1261 attgccgggg attttcccat ctgtgtggtg cccatgatgt accaggacct ggaatggcat
     1321 gatgttcctt tctggagtta tttctgccag gtctccgatt ccaccacttc gtacggatcc
     1381 tattcagggg ccgttcccaa cgaaaaaata acctggggaa aactggacat tcatactccg
     1441 aaatacatcg tggaaagcga tgccaccatt gtggtcccgc ttatatttgc ctggattctg
     1501 ggctggtaaa cggaacgacc aggcaaataa acggcaaacc ggattccttg ccgggaaggc
     1561 aaagcacaaa attcacagat gaaaaagctt gggaattata tcgccggaaa atggatagaa
     1621 ggagagggag aaggccagac cctctttgat gcggttaccg ggcaggcgat ctgccaggcc
     1681 ggaaccgggg gactggattt tcagcaaatg ctggattacg cgcgcaatac cggcaatcct
     1741 gctttacgga aaatgaattt tcatgaaagg ggccggatgt taaaggacct ggcccttcat
     1801 cttcaaaaac aggttgagct gttttaccgg atcagttatc agaccggcgc cacgcgcgcc
     1861 gacagctgga tagatattga aggcggcatc ggaaatctgt tttcttatgc tgcgttgcgc
     1921 agaaaatttc ctgatcagcc tttttgtacc gatggggatc ccgtcaacct cagcaagaac
     1981 aacagcttca tgggccatca cctgctggtt cccaaagaag gggtggccgt tcatatcaat
     2041 gcctataatt ttccggtatg gggaatgctg gaaaagatcg ccgtcaattt tctggccggc
     2101 gtaccggcta tcgtaaaacc ggcgaccgtt acttcttatt taacggaagc cgtcgtgcgg
     2161 gaaattcatg cttccgggat ccttccgccc ggcgccctgc agctgatctg cggcagtacc
     2221 ggtaacctgc tggacctgct tacggcccag gacacggtta cttttacggg ttcggcctcc
     2281 accggcctga agctgaaatc caacccgaat attctccggg agaatgtctc tttcaccatg
     2341 gaagcagatt ccctgaattg cattgtcctg ggagaagatg cagatccttc caaacccgaa
     2401 tgggatattt ttgtgaaaga ggtcagaaag gaaatgacgg tcaaggcagg acaaaaatgt
     2461 actgccatcc gccggatttt tgttccggaa aacaaactgg aggagatggg caaatccatt
     2521 tttaaatcgc ttgcacagac cgttatcggg aatccttcca atgaaaaagt gaggatgggt
     2581 tcgcttgccg gccgggaaca acggcgggaa gtgcgggaac aggtgaagaa attactgaat
     2641 tcttcccgga tcatttacgg ctcggtgaac gaagtggaac tactggacgc ggatccggac
     2701 aaaggggcct ttatgagccc cctgctgttg ttgaacgagc aacctttttc cggagaagaa
     2761 gcacactgta ttgaagcctt tggaccggta agtacgttga tgccttataa aaacatggat
     2821 gaagccattg ccctgtcaaa aaaaggaaag ggatccttat gcagctccat tgttacggcc
     2881 gatgatcgta tcgcaaaaca atatgtgacc agggcagcca cgcatcatgg cagaatactg
     2941 atcttaaaca gggattgcgc cagggaaagc acgggccatg gttcaccctt gcccatgctg
     3001 gttcatggcg gcccgggccg cgccggcgga ggggaagaaa tgggcggcgt acggggcgtg
     3061 aagcattata tgcagcgggt ggccattcag ggttcaccca ccacactcac cgccattact
     3121 tcggtgtatc aaccccacgc caggcaaaag ctggaagaaa aacatccgtt cagaaaatat
     3181 tttgaagaac tgcagatcgg ggatacggcc cttacccaca agcgtacgat aacggaatcg
     3241 gatatctcgc agtttgccaa tctcagctgg gaccattttt atccgcatac cgattttact
     3301 tccctggaag gaactatttt tgaaaaaccg gtggcgcacg gatactttat cctgagcgcg
     3361 gctgccggat tgtttgtgga tgcggccaag gggccggtat tgttaaatta cggtatagag
     3421 gaatgccgtt ttgtaaaacc ggtatacgcg ggaacaacca tcggcgtgcg gttgacctgc
     3481 caggaaaaaa cgggtcagga attaaaagaa ggggacgctg ttccaaaggg gatcgtgaag
     3541 tggtttgtgg acgtatatga tgatacgggt gaatccgtga tgattgccag cattctcact
     3601 atggtcagaa aaaaatccta aatgatatga acgggaatcc atccggctat gtgcggtcgg
     3661 aaaggcatgg ggcatccatg accatcgagt tttttcatcc acagagtaat tccctgcccg
     3721 gcgccctgct aggtgaactg gcaacgcaga tccacagtgc gggtcttgac gaagagatcc
     3781 gcgtgatcct cctgcggtcc gtgggagaaa aggcgttttg tgcaggcgct tcatttgctg
     3841 aattatcccg gatccggacg gcccgggaag gagaagattt cttcaatgga tttgcgcatg
     3901 ttctgaacag catgcggaaa tgcccgaaat tcattatagc ccgggttcag ggaaaatgtg
     3961 ttggtggcgg tgccggactt gttgccgctg ccgattatgc cattgcctta accggcgctg
     4021 aaataaaact cagcgaactg gccattgg
//
LOCUS       DASYCU010000216         5263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5124273, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000216 DASYCU010000000
VERSION     DASYCU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5124273"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VGM24_04405"
     CDS             <1..674
                     /locus_tag="VGM24_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154638.1"
                     /translation="VRKNKILVAVLVLCVVAVIVFSKQLGLNHYTDVFSRQFVKGVTL
                     PEMLEWHFSEWGEIFINISVLKIIPFLPSPFGKWLFILIGIIMVAGFIYICFIKSRIP
                     FIVRAYLFFYIVLMFNWPFPDPRFWVPVIPLIAAVIAQSSFSAGKWVKVPLTFLFIVY
                     AGFGLLSMSYMTYTSLNKKVLARNQAAGVYRNEYETHFFGKPLSDTATRVDPFVLSVL
                     NRFDK"
     gene            complement(715..1218)
                     /locus_tag="VGM24_04410"
     CDS             complement(715..1218)
                     /locus_tag="VGM24_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154639.1"
                     /translation="MGKTKWIWILGAILMVGMISCKKGDTGPAGPAGPAGPDSVVYSA
                     WIPLSPTYNADDTLFEQTISAPSITQAILDSGVILSYVNFGSTGSYDVVPVSAVNYLI
                     QEDFSVGSISLLIPPSYDLSGLPYRYVTIPGSKIEGNRSTGAVKGITIQELKTMPYAK
                     VQALLEN"
     gene            complement(1354..1974)
                     /locus_tag="VGM24_04415"
     CDS             complement(1354..1974)
                     /locus_tag="VGM24_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY4154640.1"
                     /translation="MQTAEAAYFPHLSKDKKLGKLLAQQEAFTLGKRTPVYLHLCNSI
                     MGQQLSTKVAAVIRKRFMRLYSGKPTPQKILDTSYETLRSIGLSGAKTAYIQNVARFT
                     LEKGMDHKLLGKMENEELIEHFTQIKGVGRWTAEMLLMFTLGREDVFAVDDLGIQHAM
                     TRVYKLDKTDKKKLKEEMLRISAKWSPYRTYACLHLWHHKDNVPVI"
     gene            complement(1989..2945)
                     /locus_tag="VGM24_04420"
     CDS             complement(1989..2945)
                     /locus_tag="VGM24_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY4154641.1"
                     /translation="MSISGNRRILFFLIGLAGITGWGCNKNAPNSIDYKTSTISYILA
                     NGSNTSFFESVILKANLDTIFNGAGPFTVFVPTNDAFNQAGISQPELSTYSSEEAKNL
                     VLYHTVAGTALTSVDFIGAKEKKIIMANGDSVFITGDSNRIFVNGVPVTAVDVKAANG
                     VMHALQGVLLAPSKNLLELINADSTLSFLANALLLATASPDSLTSTLAGGGPYTFFAP
                     DNDAFRILGYNSPQDLGSVNPDSLRLMILSQLVPQRLFSYDIPDSSSLQTVNDSTLYF
                     NSAGISTQVQLKGHESFSNVVSSNQMATNGILFKTDAILTQQ"
     gene            complement(3029..4519)
                     /gene="cysS"
                     /locus_tag="VGM24_04425"
     CDS             complement(3029..4519)
                     /gene="cysS"
                     /locus_tag="VGM24_04425"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY4154642.1"
                     /translation="MSNLKVYNSYSRQKESFVPLTPHHVGMYVCGPTVSGESHLGHAR
                     GFITFDVIYRYLLYLGYKVRYVRNITDAGHFEEEGREAEDKISKKAVIEKLEPMELVQ
                     KYTNLFHWAMRQFNNLDPSIEPTATGHIIEQQEMIKKIIVAGYGYAVNGSVYFDVKKY
                     AGKYPYGKLSGRKLDELLETTRDLESQEEKHNRQDFALWKAAPPGHIMRWNSPWGEGF
                     PGWHIECSAMSTKYLGDQFDIHGGGMDLMFPHHESEIAQSMICNHEVAARYWLHNNMI
                     TINGKKMGKSYNNVITLTDMFSGNSPLLAQAYHPMTIRFFILQTNYRSTLDFGNEALQ
                     AAEKGLRRLWDSYRELQKMTTDPQKEINPQLETQFNSLCHEFDDFMNDDFNTAKVLAN
                     MFMLVNHINSFRSGDLEKTTLSPETLGYVQKQFRCYLEEVMGLTEDSGTDTERLQGIM
                     DLLIDIRREARAKKDYRTSDRIRNQLTEIGILLKDEKDGRVSFTLE"
     gene            complement(4542..>5263)
                     /locus_tag="VGM24_04430"
     CDS             complement(4542..>5263)
                     /locus_tag="VGM24_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY4154643.1"
                     /translation="DANILCFQEFYEPDDSTQSNIRYIRDKLHFPYYFFSRDTHNNTG
                     KYETGVIIFSKYPILWNVRHVFSSDKTFGRESLIEADIAVNGKRIRVSTAHLQSVLFK
                     SKDFRDVEIIRNAQDNMLPASRSLAKKLKYALSLRGYQADTVRKYLDSSPYPVIMCGD
                     FNDVPNSYTYFHIKGNRQDAFIARNFGIGRTYIHISPTLRIDYILPGTEFKVLQSTKI
                     VTPFSDHHPVLADLLLSDEKR"
BASE COUNT         1231 a         1256 c         1289 g         1487 t
ORIGIN      
        1 tggtcaggaa gaataaaata ctggtggcgg tgctggtact ctgtgtggtc gccgtgattg
       61 tgttttcgaa acagctcggg ctcaatcact ataccgatgt cttctccagg caatttgtga
      121 aaggggtaac gcttcctgaa atgctggaat ggcatttttc agaatggggg gagatcttca
      181 taaatatttc tgttttgaaa atcatccctt ttctgccatc gccctttggt aaatggctgt
      241 tcattctgat tgggatcatc atggtcgccg gatttattta tatctgtttt ataaagagcc
      301 gtatcccgtt tatcgtccgg gcttacctgt ttttctatat cgtgctgatg tttaactggc
      361 cctttcctga cccccgcttc tgggttccgg ttattccgct gattgcggca gtgattgcac
      421 aaagttcttt cagcgcagga aaatgggtaa aggtcccgct tacctttctt tttattgtat
      481 atgccggatt tggccttctt tcgatgagct acatgaccta cacctccctg aataaaaaag
      541 ttttagcgag gaaccaggct gccggcgtgt accggaacga atatgaaacg catttttttg
      601 gcaagccgct gtccgacacg gccacccggg tggatccgtt tgtactgagt gtgttgaacc
      661 ggtttgacaa ataaaaagcg ggattacccg ctttttactt tcagattttt ctttttaatt
      721 ctccagcagg gcctgcactt ttgcgtaagg cattgttttc aattcctgta tggtaattcc
      781 tttaacggcg cccgtgctcc tgttcccttc tattttggaa ccgggtatgg ttacataccg
      841 gtagggcagg ccggataaat cgtaggaagg agggatcagg aggctgatgg agcccactga
      901 aaaatcttcc tgtattaaat aattcactgc ggaaacgggt accacatcat aagagccggt
      961 gcttccaaaa ttgacatagg aaaggatcac gccactgtcc agaatcgcct gtgtgatcga
     1021 aggggcgctg atcgtctgtt caaataaagt gtcgtcggcg ttgtaagtcg gactaagggg
     1081 aatccaggct gaataaacca cactgtcagg acccgcaggg ccggccggac cggccggacc
     1141 ggtgtcaccc tttttgcagc tgatcattcc aaccatcagg atggcgccca gtatccagat
     1201 ccatttagtt tttcccatat tcaatttttt taatgtttat ttttattatt cctggcctgc
     1261 aagataattt atttgccagt ccgtaaaatg aatttttgcc cgggcttttt aaaggatacg
     1321 ggaatatggc atttgggttg cctttgaccg cggtcagatt accggcacat tatccttatg
     1381 atgccataaa tgaaggcagg catacgtgcg gtaaggggac catttcgcgg agatccggag
     1441 catctcttcc tttaattttt tcttatccgt tttatccagt ttgtagaccc gggtcatggc
     1501 atgctggatc cccaggtcat ccaccgcaaa aacgtcctcc ctgcccagag tgaacatcaa
     1561 cagcatttcg gcggtccacc ttccgactcc tttgatctgg gtgaaatgtt ctatcaattc
     1621 ctcgttttcc attttgccga gcagtttatg gtccataccc ttctccaggg taaaccgggc
     1681 cacattttga atataagcgg ttttggcgcc ggacagcccg atggaccgca gggtttcata
     1741 agacgtatcc agaatttttt gcggcgtggg cttaccggaa tataatctca taaaccgctt
     1801 ccggattacc gccgccactt tagtggaaag ctgctgtccc ataatggaat tacataaatg
     1861 caggtaaacg ggggtccgtt tgcccaaggt gaatgcctcc tgctgtgcca gcagtttgcc
     1921 cagtttttta tctttcgaaa ggtgcggaaa ataagccgct tctgcggttt gcatgcaggc
     1981 aaattaattt attgctgagt caatatggca tctgttttaa acaggatccc gttcgttgcc
     2041 atctggtttg atgaaaccac gttggagaaa ctttcatgcc cttttagctg cacctgggtg
     2101 ctgatgccgg ccgagttgaa atagagcgta ctgtcattta ccgtctgcaa agagctgctg
     2161 tcgggaatat cataggagaa cagacgctgg ggaaccaatt gggataagat catcagacga
     2221 agggaatccg ggttcactga acccaggtcc tgcggcgagt tgtaccctaa aatccggaat
     2281 gcgtcattgt cgggcgcaaa aaaagtatac ggtcctccgc cggcgagggt gctggtcagg
     2341 ctgtccggtg atgcggtcgc cagcagcagg gcattggcaa gaaaactgag ggtgctgtcg
     2401 gcattgatca gctccagcag attttttgac ggagccagca gaacgccctg taatgcatgc
     2461 ataactccat tggctgcttt cacgtccact gcagtgaccg gaacgccgtt cacaaagatg
     2521 cggttggaat cgcccgtaat gaataccgag tcgccgttgg ccatgattat cttcttttcc
     2581 tttgccccga taaaatcaac cgaagtaagg gccgtgccgg caacggtatg atagagcacc
     2641 aggttttttg cttcttcact gctgtaggtg cttaattccg gctgactgat tccggcctgg
     2701 ttaaaggcgt cgttggtggg aacaaaaacg gtgaatggtc cggccccgtt gaagatcgta
     2761 tccaggttcg ccttcaggat aacggattca aaaaagctgg tattgctccc gtttgccagg
     2821 atataggaaa tggtggacgt tttatagtcg atcgaattcg gcgcattctt attacagccc
     2881 catccggtaa tcccggccag gccgatcaaa aaaaacaata tccttcggtt acctgaaatg
     2941 ctcatacaag gttttatttg gggtccaccc ggctcccgcc cctgctatac gtataaaaat
     3001 cgtttatggt atgctgccgc ctttaatttt attccagggt aaagcttacc cggccgtcct
     3061 tctcgtcctt caggagaatg cctatttccg tcaattggtt acgaatgcgg tcggaggtcc
     3121 gataatcctt tttggcccgg gcttcgcgcc ggatatcgat cagcaggtcc ataatgccct
     3181 gcagcctttc cgtatccgta ccgctgtcct ccgtcaagcc catcacctct tccagataac
     3241 acctgaactg tttttgcacg taaccgagcg tttccggaga aagggtcgtt ttttccaggt
     3301 caccgctccg gaatgagttg atatgattga ccagcatgaa catattggcc agcactttgg
     3361 ccgtattaaa atcatcgttc atgaagtcgt caaattcatg acaaagggaa ttgaactggg
     3421 tctccagctg gggattgatc tccttttggg gatccgtggt cattttctga agttcccggt
     3481 atgaatccca taatcgcctc agtccttttt cagctgcctg aagggcttca ttaccgaaat
     3541 ccagggtgct ccggtagttg gtctgtaaaa taaaaaaacg aatggtcatc gggtgataag
     3601 cctgggcgag aaggggactg tttccggaga acatatccgt aagcgtgatc acattattat
     3661 agctctttcc catttttttc ccgttgatcg taatcatatt attgtgcagc cagtaacggg
     3721 ctgccacctc gtgattacag atcatactct gggcaatttc actttcatgg tgcggaaaca
     3781 tcaggtccat acccccgcca tggatatcga actggtctcc gagatatttt gtgctcatgg
     3841 cggaacattc aatatgccat ccgggaaatc cttctcccca ggggctgttc cagcgcatga
     3901 tatggccggg aggcgccgct ttccataagg caaagtcctg gcgattgtgt ttttcttcct
     3961 ggctttccag gtcgcgtgtg gtttccagca gctcatccag cttgcggccg ctgagctttc
     4021 cgtagggata cttcccggca tatttcttta catcgaaata gacggagccg ttcacggcat
     4081 agccatagcc tgccacgatg atctttttaa tcatttcctg ctgctcgatg atatggccgg
     4141 tagcagtcgg ttcaatactg gggtccaggt tgttgaactg ccgcattgcc caatgaaaga
     4201 gattggtgta tttctgcacc agctccatgg gctccagctt ctcgatcact gcctttttgg
     4261 agattttgtc ttccgcttcc ctgccttctt cttcaaaatg acccgcatcg gtgatattgc
     4321 ggacgtaccg taccttatag cccaggtaca ggagataccg gtagatcaca tcgaaggtga
     4381 taaagcccct ggcatgcccc agatggctct ccccgcttac cgtgggtccg catacataca
     4441 tccccacatg atggggcgtc aacggaacaa atgactcttt ttgcctgctg taagaattat
     4501 agacttttaa gttactcatg ccttcgccca aatataaaca tttatcgctt ttcgtctgaa
     4561 aggagaaggt cggcaagcac cggatgatga tcggagaaag gggttactat ttttgtggat
     4621 tgcaagactt taaattcggt tcccggaaga atataatcga tgcgcaacgt gggggaaata
     4681 tgaatatagg tcctgccgat accaaagttt ctggctatga aggcatcctg cctgttcccc
     4741 ttaatatgaa aataggtata tgaattcggc acatcattga aatcgccgca catgatcacc
     4801 ggataaggac ttgaatccag gtattttctt acggtatctg cctggtatcc gcgcagactg
     4861 agcgcatatt tcaacttctt tgccagggaa cgggaggcgg gtaacatatt gtcctgtgca
     4921 ttgcgaatga tttcgacatc ccggaaatcc ttggatttga aaagaacgga ctgcagatgg
     4981 gccgtgctca cccggatccg ttttccattc accgcgatat ccgcttcgat caggctttcc
     5041 ctgccaaagg ttttgtctga agaaaaaaca tgacggacat tccagaggat cggatatttg
     5101 gaaaatatga tcacgcccgt ttcatatttg ccggtgttat tgtgcgtatc gcgggaaaag
     5161 aaataataag ggaaatgaag cttgtctctg atatagcgga tattggattg ggtggaatca
     5221 tcgggttcat aaaattcctg aaagcagagg atgttggcat cct
//
LOCUS       DASYCU010000217         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3238879, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000217 DASYCU010000000
VERSION     DASYCU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238879"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VGM24_04435"
     CDS             complement(<1..214)
                     /locus_tag="VGM24_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY4154644.1"
                     /translation="MINKIGIIGGGNLGMAIAEGLIQSGYLNPGHILITRRSVDQLHP
                     LERKGVMVSGNNEDALNYGHLIILAVK"
     gene            complement(219..1211)
                     /gene="rfaD"
                     /locus_tag="VGM24_04440"
     CDS             complement(219..1211)
                     /gene="rfaD"
                     /locus_tag="VGM24_04440"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY4154645.1"
                     /translation="MTKNSVIVITGAAGFIGSCLTGFLNERGFEQLILVDEFGRIDKI
                     RNLEGKKYIAKIERDDFFDWLQAKAPAIAFIFHIGARTDTTEFDYAVHERLNVEYSQK
                     VWDYCMVKNIPLLYASSAATYGAGEYGYPDDHQLPFKLKPLNAYGISKNEFDKWAIRE
                     KSHPPFWAGLKFFNVYGPNEYHKGRMASVIWHSFRQISKEGEVKLFRSHRPDYRDGQQ
                     LRDFIYVKDILKVCFWFMENYRSPGIPSGLYNLGTGKARTFEDLVRAVFAGVGKPVKI
                     KYIDMPEDIRDKYQYFTEADMKKLIAAGYKEPFYSLENGIGDYVKNYLTPNNYN"
     gene            complement(1208..>2459)
                     /locus_tag="VGM24_04445"
     CDS             complement(1208..>2459)
                     /locus_tag="VGM24_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY4154646.1"
                     /translation="KAVLEVFEKWDLPCSNIGEVTDDKMLRFYVDGKLEAAIPAFELV
                     LGGGAPQYSREYREPAYFSRIKAFDPDGIPVPENLVAIAEILIRLPSIASKRWISVQY
                     DSTVGAANCSTNEPSDAAIVLVKGTSKAIVMTTDCNSRYVLADPYRGTMMAVAEAARN
                     IVCSGGTPLGVTNCLNFGNPYQPEVYYQFVQAIKGMGEACLKFDTPVTGGNVSFYNQH
                     PEGAVYPTPTIGMVGLLENVQDKMTLFFKQAGDLIVLTGRSRNDIHSSAYLYAVHGVE
                     YSPAPHFELEEEFKLQEMISRLIKDKLIESAHDVAEGGLFVALAECGFQQGLGFAVSS
                     GDKEIRPDAYWFGESQSRVVISLKKEKLPALKKALGNFPYEILGEVSPSVVSVNGADW
                     GSIQNWKFRYDTAIEREMESIVEA"
BASE COUNT          570 a          661 c          572 g          656 t
ORIGIN      
        1 gtttaaccgc aaggatgatc aggtggccgt agttcagggc atcttcattg ttcccgctca
       61 ccattacgcc ctttctctcc agcggatgaa gctgatcaac cgaccggcgg gtaatgagaa
      121 tatgccccgg atttaaatat ccgctttgaa tcaatccttc cgcaatggcc atgcccaggt
      181 tccctccccc gataattccg attttattaa tcataatatt agttgtagtt attgggcgtc
      241 agataatttt ttacgtaatc gcctatgccg ttctccaggc tgtaaaaggg ttctttataa
      301 ccggcggcga tcagcttttt catgtctgct tctgtaaagt actgatactt gtcgcggatg
      361 tcttccggca tgtcaatata ttttatttta accggtttgc ctacgccggc gaaaacggcc
      421 cgcaccaggt cttcaaaggt ccttgctttt ccggtgccca gattgtacag accgctggga
      481 atgcccgggg accggtaatt ttccatgaac cagaaacaga ctttcagaat atcctttacg
      541 tagataaaat cccggagctg ctgcccgtcc cggtaatccg ggcggtggga tctaaacagc
      601 ttgacctcgc cctctttgct gatctgccgg aaggaatgcc agatcacgct ggccatgcgg
      661 cccttgtgat attcattggg cccgtacaca ttgaaaaatt ttaagcccgc ccagaacggc
      721 ggatggcttt tttcccggat cgcccattta tcgaattcgt tttttgaaat cccgtaggca
      781 ttcaggggtt ttaatttaaa gggaagctgg tggtcatccg gatacccgta ttcccctgca
      841 ccatacgtcg cggccgagga agcatacagc aggggaatgt tttttaccat gcaataatcc
      901 cagaccttct gcgagtattc cacattcagc cgttcgtgca ccgcataatc aaattccgta
      961 gtatccgttc tcgctccgat atgaaatatg aaagctatag cgggggcttt tgcctgcagc
     1021 cagtcaaaaa aatcgtcccg ctcgattttg gcgatatact tctttccttc caggttccgg
     1081 atcttgtcga tccggccaaa ttcatctacc aggatcagtt gctcaaatcc tctttcattc
     1141 aggaatcccg tcaggcaact gccgatgaaa cccgcagcac cggtaattac aatgactgaa
     1201 ttttttgtca tgcttcaaca attgattcca tttccctttc aatggcggta tcataacgga
     1261 atttccagtt ttggatcgaa ccccagtccg ctccattcac cgaaacaacg gaagggctca
     1321 cttcccccag tatttcataa gggaaatttc cgagtgcttt tttcagggca ggaagttttt
     1381 ctttcttcag gctgatcact acccggctct gcgattcacc aaaccagtat gcgtcgggcc
     1441 ggatctcttt atcgccggaa gaaaccgcaa atcccagtcc ctgctgaaaa ccgcattcag
     1501 caagcgctac gaataagccg ccttcggcaa cgtcgtgggc ggattcgatc agtttatctt
     1561 tgatcaggcg ggaaatcatt tcctgcagtt taaattcctc ttccaattca aaatgagggg
     1621 caggactgta ttccactccg tgaacggcat agaggtaggc ggaggaatga atatcgttgc
     1681 gggagcggcc ggtcagtacg atcaggtcgc cggcttgttt aaaaaacagg gtcattttat
     1741 cctgtacatt ttccaggagc ccgaccatac cgatcgttgg cgtcggatac actgctccct
     1801 cgggatgctg attatagaaa ctcacatttc ctccggtcac gggcgtatcg aatttcaaac
     1861 aggcttcccc cataccttta atcgcctgca caaactggta atatacttcc ggctgatacg
     1921 gattgccgaa attcaggcag ttggtaaccc ccaggggtgt accccccgaa caaacaatat
     1981 tccgcgccgc ttccgccacc gccatcatgg ttccccggta gggatcggcg agtacatagc
     2041 ggctattgca atcggtggtc atcacaatcg ctttggaagt tcctttaacc aatacgatgg
     2101 cggcgtccga tggttcgttg gtagaacaat tggcggctcc cacggtactg tcgtattgca
     2161 cgctgatcca ccgtttggag gcgatggaag gcaggcggat gagtatttcg gcaatggcca
     2221 ccaggttttc cggaaccggt attccgtccg ggtcaaacgc ttttatccgg ctgaaataag
     2281 ccggttcgcg atactcccgg ctgtattgcg gagcgccgcc gcccaacacg agttcaaaag
     2341 ccggaatagc cgcttccagc tttccgtcca cataaaaacg cagcatttta tcgtcggtta
     2401 cttcgccgat attagagcag ggcagatccc atttttcgaa tacctcgagg actgctttc
//
LOCUS       DASYCU010000218         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1934742, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000218 DASYCU010000000
VERSION     DASYCU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934742"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VGM24_04450"
     CDS             complement(<1..629)
                     /locus_tag="VGM24_04450"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEY4154647.1"
                     /translation="MDIIIIGSGNVATRLGRKFREAGHRILQVFSRNPSHAVLLAEIL
                     NATPISALSEMKTGADLILLAISDNAYPEFVHELSPTGSFVVHTAGSVPMEVLKNTSS
                     RYGVLYPLQSFRRELEDQPEFPLLIDANNPGSLEELKEMAAGLSKTILPAGNEMRLKY
                     HLAAVLAGNFSNHLYSLTESWCRREEIDFGLLQPLIRETAGRLLNELPE"
     gene            complement(673..1251)
                     /gene="infC"
                     /locus_tag="VGM24_04455"
     CDS             complement(673..1251)
                     /gene="infC"
                     /locus_tag="VGM24_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY4154648.1"
                     /translation="MAFPPRPPRGTFNPRFRKEQQQEHRTNNMIRVPQVRLVGENVTV
                     GVYPTQEALRMAQDQALDLVEISPNADPPVCKIIDYNKFLYEKKRKEKEMKAKSKASE
                     VKEIRFTPNTDDHDFDFKAKHAEKFLKDGDKVKAYVQFKGRAIQFRERGELLLLKFAE
                     RLNEVGVLEGMPKMEGKRMLAIWAPKSQKKKT"
     gene            complement(1300..3234)
                     /gene="thrS"
                     /locus_tag="VGM24_04460"
     CDS             complement(1300..3234)
                     /gene="thrS"
                     /locus_tag="VGM24_04460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY4154649.1"
                     /translation="MIKITLPDGAVKEFEAGTSSLQVAKSISEGLARKVIAASVNGEV
                     WDASRPIGKDATLKLLTWTDPEGKSTFWHSSAHLLAEAVESLFPGVKFWVGPAVDNGF
                     YYDMDLGGRQITEEDLRKLESRMSELAGKNSVYIRKPISKEEALKYFREKGDEYKLDL
                     LQNLKDGEITFYTQGNFTDLCRGPHIPDTGFIKAVKLTNIAGAYWKGDEKNKQLTRIY
                     GVSFPSRKELDEYLLLLEEAKKRDHRKLGKEMGIYTMDDDVGPGLVLWMPNGTIIIEE
                     LEKLAKETEREAGYQRVVTPHIGKENLYLTSGHLPYYQDSMYPPMELDGVKYYLKAMN
                     CPHHHKIFAAEPRSYKDLPLRLAEYGTCYRYEQSGELFGLMRVRCLHMNDAHIYCSKE
                     QFAQEFQAVNAMYLKYFRIFGIEKYVMRLSLHDPAELGKKYINEPELWLETEAMVRRV
                     LKETGTPYVEVAGEGAFYGPKIDVQIWSTIGREFTLATNQVDFTQGKRFNLEYTTPEN
                     HPATPLIIHRAPLGTHERFIGFLLEHYAGKFPLWLAPQQVKLLPISDKFVPYAQTVLE
                     SLKKADIRAGIDERSEKIGKKIRDTELARVPYMLIIGEKEMNEQKISVRRQGRGDLGI
                     QDLETFIRDAKTEITLRSGG"
     gene            3341..4519
                     /locus_tag="VGM24_04465"
     CDS             3341..4519
                     /locus_tag="VGM24_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154650.1"
                     /translation="MKMKPHFLPVILLLACFIPHPGSAQDITGIWRGHFRSNDGFERL
                     TGYDDRYKIEVQIAQTNQIFQAVTYSYKNTEFYGKAEASGTVNPKTKKVLLRELKILE
                     VRMADGDVCIMTCFLQYSKLGEEEFLQGTYTSTSARDSLSGCGKGSIFLHKVITSDFQ
                     KESFLEKREKELLAAKEKKAGSANKPQKKSVPHSTAKVPDLSRNKPVAKPLAKKTGPP
                     AKSLAKSGRPGSSAKKSNPVPQAERKSVSLVPEQRHVNPDSSFKIENKAIPVIIPRVL
                     ADRSNELVRAITVNNTEVHLNIYDDGAIDNDTVSVYYDNKLIVSRARISDQPVKVIIQ
                     VEPSDHPHELVMVAENLGDIPPNTSLMVVRDGDKKYEVRIVSTEQKNAVISFQYKKIE
                     "
     gene            complement(4523..>5038)
                     /locus_tag="VGM24_04470"
     CDS             complement(4523..>5038)
                     /locus_tag="VGM24_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY4154651.1"
                     /translation="VRILKTVGFNPRRTIRIALWGGEETGLFGSKNYVKNHFTDTVTK
                     KYNAAGDKLSVYLNLDNGSGKIRGIYLQGNEAAKPVFEKWFEPFHDLGAATLSLRNTG
                     GTDFLSFDAVGLPGFQFIQDPMEYGSRTHHSNMDTYDHLSGDDLKQAATIIASFVYDA
                     AQMEGKLPRKK"
BASE COUNT         1155 a         1310 c         1220 g         1353 t
ORIGIN      
        1 ttctccggca attcattcag cagacggccg gccgtttccc ggatcagtgg ctgcaacagg
       61 ccaaaatcaa tctcctcccg gcggcaccag gattccgtga gggagtatag atggttactg
      121 aaattgccgg ccagcacagc ggccagatga tattttaaac gcatttcatt tccggccgga
      181 agaatggttt tgctcaggcc ggccgccatt tcttttaatt cctccaggct tccgggattg
      241 ttggcatcga tcaataaggg aaattcaggc tggtcttcca gttcccgccg gaaactctgc
      301 aacgggtaga gcacgccgta ccgggaactg gtattcttca atacctccat gggcaccgaa
      361 ccggcggtat gcacaacaaa agatcctgta ggcgaaagtt cgtggacgaa ttccgggtag
      421 gcattgtccg atatcgcaag gaggatcagg tccgccccgg tcttcatttc gctgagtgcg
      481 gaaatgggcg ttgcgtttag aatttctgcg aggagaacag catgggacgg gttgcggctg
      541 aatacctgga gaatgcggtg accggcttcc cggaatttgc gtcccagccg ggttgccaca
      601 ttacctgagc cgatgatgat gatgtccatg gaccggatga ccggaatagt ttagcaatga
      661 gatgaccacc ggtcaggtct ttttcttctg ggacttgggt gcccagatgg ccagcattct
      721 tttaccttcc atcttcggca taccctcgag cacgcctacc tcattcagcc gctccgcaaa
      781 tttcagcagg agcaattcgc ctctttcccg gaactggatt gcccggcctt taaactggac
      841 ataggctttt accttgtctc cgtctttgag gaatttctcg gcatgttttg ccttgaaatc
      901 aaaatcatga tcgtccgtat tgggggtgaa ccggatctcc tttacctctg aagccttgct
      961 cttggctttc atttcctttt ccttccgctt cttttcgtaa agaaatttat tgtagtcaat
     1021 gatcttgcat accggcggat ctgcattggg ggagatctcc accaggtcca gggcctgatc
     1081 ctgggccatc ctcagcgctt cctgggtagg ataaacgcca acggttacgt tttctccaac
     1141 gagcctgacc tgcggcacgc ggatcatatt attggtgcgg tgttcctgtt gctgttcctt
     1201 tctgaatcgg ggattaaaag tacctctcgg aggtctcggt ggaaatgcca tttataggtt
     1261 ttaagtgaac gatagttaag acgggggctg tttatttttt tatcctcccg acctcaacgt
     1321 aatttctgtt tttgcgtcgc gaatgaatgt ttctaaatcc tgaattccca gatctcccct
     1381 gccctgccgg cgtacagaaa ttttctgctc attcatttct ttttcaccga tgatcaacat
     1441 atagggaacg cgcgccagtt ctgtgtcgcg gatctttttc cctatttttt cactgcgctc
     1501 atcaatgcct gcgcgaatat ctgctttttt aagggattcc aaaacagttt gtgcgtaagg
     1561 gacaaatttg tcgcttatgg gtagcaattt aacctgttgg ggggctaacc acaaggggaa
     1621 tttccctgca taatgttcca gtaaaaaccc gataaaccgc tcgtgggtac ccagaggcgc
     1681 ccggtgaatg atcagggggg ttgccgggtg attctctggg gtggtatact ccaggttaaa
     1741 ccttttaccc tgggtgaaat ccacctggtt ggtcgccagg gtaaattcac gcccgatcgt
     1801 gctccagatc tgtacatcaa tttttggtcc gtaaaacgcc ccttcgcccg ccacttccac
     1861 atagggagtg ccggtttcct tgagcaccct tcgcaccatg gcctccgttt ccagccagag
     1921 ttccggttcg ttgatatatt ttttaccgag ttcggccgga tcgtggaggc ttagccgcat
     1981 cacatatttc tcaatgccga aaatgcggaa gtatttgagg tacatggcat tcaccgcctg
     2041 gaattcctgg gcaaactgtt ccttgctgca atagatatgg gcatcgttca tgtggagaca
     2101 gcggacccgc atcagtccga ataattcccc gctttgctca tagcggtagc aggttccgta
     2161 ttccgcgagc ctcagcggaa ggtctttata gcttctgggt tcggccgcga aaatcttgtg
     2221 gtggtgtggg caattcattg ccttcagata atattttacc ccatccagct ccatcggagg
     2281 gtacatgcta tcctggtaat agggtaaatg cccgctggtc aggtagagat tttccttgcc
     2341 aatatgaggg gtgaccaccc gctggtatcc agcctccctt tcggtttcct tggccagctt
     2401 ttccagttct tcgataatga tggttccgtt cggcatccag aggaccagtc cggggcccac
     2461 gtcatcatcc atggtgtaaa tgcccatttc ttttcccagc ttgcgatgat cccgcttttt
     2521 ggcttcttcc agcaggagca ggtactcatc cagttctttt ctggacggaa aactgacccc
     2581 atagatccgg gtgagctgtt tgttcttttc gtctcctttc cagtacgcgc cggcaatatt
     2641 ggttagtttg accgccttga tgaagccggt atcgggtatg tgcggtcctc ggcagagatc
     2701 cgtaaagttt ccctgcgtgt aaaaagtaat ctccccgtct tttaaattct gaagcagatc
     2761 gagtttatac tcgtcccctt tctcccggaa atatttcagt gcttcttctt tggaaatggg
     2821 tttacggata taaacgctgt tttttccggc cagctcactc atcctggatt ccagcttccg
     2881 caagtcttct tccgtaatct gccggccccc gagatccata tcataataga acccgttatc
     2941 gaccgccgga ccgacccaga atttaacccc cggaaacaga gactccacgg cttccgccag
     3001 caaatgggcg gaagaatgcc aaaaggtgga ctttccttcc ggatcagtcc aggtcaacag
     3061 tttgagcgtg gcatcctttc cgatcgggcg cgaggcatcc cacacctctc cgtttacgga
     3121 agccgcaatc acttttctgg ccaatccttc gctgatggat ttggccacct gcagcgaact
     3181 ggttcccgcc tcgaactctt ttaccgctcc gtctggcaat gtaattttaa tcatgatact
     3241 tatctggttc ctgtttaagg gttgcaaaat taacaaactc ttgccagatt aacttttcgg
     3301 tgagttgccc ggaataaaat attcaataat ttgccggtat atgaaaatga agccccattt
     3361 tcttccggtc attctcctgc tggcctgttt tattccccac ccggggtctg cacaggatat
     3421 taccggaata tggcgggggc atttccgttc caatgatgga tttgagcggc tgaccggata
     3481 cgatgaccgc tataaaatag aggtgcagat cgcccagaca aaccagatct ttcaggccgt
     3541 aacctattcc tataagaata cggaatttta tggaaaggca gaagcttccg ggaccgtaaa
     3601 tcccaaaaca aaaaaagtgc tcctgcggga actgaaaatc ctggaagtgc ggatggcaga
     3661 cggagatgtc tgtatcatga cctgtttttt acagtattcc aaactcggag aggaagaatt
     3721 cttacaggga acctatacga gcaccagtgc ccgtgattcc ttatccggtt gcggtaaggg
     3781 cagcattttc ctgcacaaag tgatcacttc cgactttcaa aaggaatcct tcctggagaa
     3841 aagggaaaag gaattattgg cggctaagga aaagaaggcc gggtctgcca ataagcccca
     3901 gaaaaaatcc gttccgcatt cgacggcaaa agtcccggat ctcagccgca ataaaccggt
     3961 tgctaaacca ctggcaaaaa aaaccggtcc ccctgccaaa tcattggcaa aatccggcag
     4021 gccgggttca tccgccaaaa aatccaatcc cgttccccag gcagaaagaa aatccgtttc
     4081 actggttccg gaacagcggc acgtaaatcc cgacagcagt tttaaaattg aaaataaagc
     4141 cattccggtc attattccca gggtgctggc cgaccggtcc aatgaactgg tccgggccat
     4201 tacggtaaat aacaccgaag tgcacctgaa catttacgat gatggtgcca ttgacaacga
     4261 tacggtgtcg gtttactacg acaacaagct cattgtttcc agagcgcgga tttcggatca
     4321 accggtgaaa gtgatcatcc aggtcgagcc ttccgaccac ccgcacgaac tggtgatggt
     4381 ggctgaaaac ctcggcgata ttccacccaa tacttccctg atggtggtgc gggacggaga
     4441 taagaagtat gaagtgcgta tcgtttccac cgaacagaaa aatgcggtga tctcttttca
     4501 gtataagaaa atagaatagc tcctattttt tccgcggcag tttcccctcc atttgcgctg
     4561 catcataaac aaaactggca ataatggttg cagcctgttt caggtcgtcg ccgctcaggt
     4621 gatcgtaggt atccatattg ctgtgatggg ttctgctccc gtattccatc ggatcctgaa
     4681 tgaactggaa tcccggaagg cccacggcat cgaatgaaag gaaatccgtt cctccggtat
     4741 tccttaggct gagcgtggca gcgcccagat catgaaacgg ttcgaaccat ttttcgaaaa
     4801 cgggtttggc ggcctcgttt ccctggaggt atattccccg gattttccct gacccgttgt
     4861 ccaggttcag gtacacggaa agcttatccc ccgctgcatt gtatttttta gtcacggtat
     4921 ccgtaaaatg attcttcacg taatttttcg atccgaagag accggtttct tccccacccc
     4981 agagtgcgat ccggatcgta cgccggggat tgaatccgac cgttttcagt atccggac
//
LOCUS       DASYCU010000219         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1062926, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000219 DASYCU010000000
VERSION     DASYCU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062926"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VGM24_04475"
     CDS             complement(<1..173)
                     /locus_tag="VGM24_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY4154652.1"
                     /translation="MKSGFVNIFGKPNAGKSTLLNSLMGEKLAIVSHKVQTTRHRIKG
                     ILTGEGYQVIFSDT"
     gene            327..860
                     /locus_tag="VGM24_04480"
     CDS             327..860
                     /locus_tag="VGM24_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY4154653.1"
                     /translation="MATVKWTMDPTHSEVQFKVRHLMISNVTGSFPKFDVEVETQDLD
                     FMTAKVKFRADTSVVTTQNEQRDQHLRSADFFDSAKYPSLSFVATRYEKVDNDSYELY
                     GDLTIRDITKNIKLDVEFGGVITDPWGNTRAGFTINGKINRKDFGLVWNALTEAGGLV
                     VGDEARVHVEVELIRQA"
     gene            893..>2154
                     /locus_tag="VGM24_04485"
     CDS             893..>2154
                     /locus_tag="VGM24_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY4154654.1"
                     /translation="MKKLIGVLFTGSVLFCTAQKKTDPVAFGNSILAENLSKDLHIVA
                     GAAFEGRETATEGQRKAAVYIEDFFRSIGLKPGNGPGFQLPYPVYQDSLLSSGVRVNE
                     DSFRLFDDYDLSIGNNYTAALGSNQVVYAGYGISDSSRDDYKELNVQGKIVLLKSGYP
                     DGYVQQQTGKRRFNPYTKQDAAQKHGALALLIIQTDFPHPPIPLKGKMYRQVYRLLNR
                     PLTIDISEKIARAIMGTGYDSVKYNSISPRSYPCNIWLSLNKKTESLQSTDVLGYLEG
                     TDLKDQWLVISAHYDHLGKQGPVIYYGADDDGSGTVSLLELAAAFSRSKAAGKGPRRS
                     ILFLANSGEEEGLWGSAYYSEHPVYPLGQTTADLNIDMIGRKEPGRKQGDSNNYVYIV
                     GDDKISSDLHPLSVSANKKYTNLELDYK"
BASE COUNT          574 a          516 c          548 g          516 t
ORIGIN      
        1 gtatcggaaa agatcacctg gtaaccttct ccggtcagga ttccttttat acgatgccgg
       61 gtggtttgca ctttatggga aacaatggcc agcttctctc ccatcagcga attcaggagg
      121 gtgctctttc ccgcattggg ttttccgaaa atatttacaa atcctgattt cataaaacgg
      181 ctgcaaaggt agacaatcag caggcaataa ccgcccctct ctgtggcggg ggttttgcag
      241 cagctttttt accgaatggc aactgaatct ttgcaatctg aaggggaatg actaatttcg
      301 ttgtttaaac attaaaatac cgcattatgg caaccgttaa atggacaatg gatcccacgc
      361 attctgaagt gcagttcaag gtgcgtcacc tgatgatctc caacgtaacc ggcagctttc
      421 cgaaatttga cgtggaggtg gaaacacagg atctggactt catgaccgct aaagtgaaat
      481 tcagggccga tacgtccgta gtgaccactc aaaatgaaca gcgggatcag catttacgaa
      541 gtgccgattt ttttgattcc gccaaatacc cttccctgtc ttttgtagcg acccggtatg
      601 aaaaagtgga caatgattcc tatgagctgt atggcgacct gaccatccgg gatattacca
      661 aaaacatcaa actggacgtg gaattcggag gggtgataac agatccatgg ggcaataccc
      721 gcgcaggatt taccatcaac ggaaaaataa accggaaaga ttttggcctg gtttggaatg
      781 ccttaacaga agccggcggt ctcgtggtgg gagatgaagc ccgcgttcat gtggaagtgg
      841 aattgatcag gcaggcctag gttattgcgg cggcttttct atatttggac ctatgaaaaa
      901 actcataggc gttttgttca ccggatctgt tcttttttgc acggcgcaga aaaaaacaga
      961 tccggttgca tttggaaaca gcatcctggc cgaaaatctg agcaaagatc ttcacatcgt
     1021 ggccggcgcc gcttttgaag gccgcgaaac ggctaccgaa gggcagcgta aggcggctgt
     1081 ctatatagaa gattttttca ggtccatcgg ccttaaaccc ggcaacgggc ccggtttcca
     1141 gctcccttat ccggtatacc aggattcact gctgtcttcc ggagtccggg taaacgagga
     1201 cagcttccgg ctctttgatg actatgacct ttccatagga aacaactata cggccgcgct
     1261 gggcagcaac caggtcgttt atgccggcta cgggatctcg gattcttccc gggatgatta
     1321 caaagaattg aatgtgcagg gaaaaatcgt tttactgaag agcggttatc ccgacgggta
     1381 tgttcagcag cagaccggca aaagaagatt caatccctat accaaacagg atgcggccca
     1441 aaaacacggg gcccttgccc tgctgatcat tcagaccgat ttcccccatc cgccgattcc
     1501 cctgaaaggg aagatgtacc ggcaggttta ccggctgctg aaccgtccgc tcacgatcga
     1561 tatatcggaa aaaatagccc gggcaattat gggaaccggc tatgattcgg ttaagtacaa
     1621 ttccatcagc cccagatcgt atccctgtaa tatctggctt tccctgaaca agaaaacaga
     1681 atcattgcaa agcaccgatg tattgggtta tctggaagga acggatctga aagatcaatg
     1741 gcttgtcatc tcggcccact atgatcattt gggtaaacag ggcccggtca tctactatgg
     1801 cgcagacgat gatggctcgg gcaccgtaag cctgctggaa ctggcagccg ccttttccag
     1861 gtcaaaagcg gcgggaaagg gaccccgcag gagcattctc tttcttgcga attccggtga
     1921 agaagaaggt ttgtggggct ccgcctatta ttcggaacat ccggtttatc cgctcggcca
     1981 gaccacggcc gacctgaaca ttgatatgat cggaagaaag gaaccgggca ggaaacaggg
     2041 agattccaat aactatgtgt atatcgtggg cgatgacaag atcagctcgg acctgcatcc
     2101 gctgagcgtg tccgcgaata aaaaatacac gaaccttgaa ctggattata aatt
//
LOCUS       DASYCU010000220         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3530046, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000220 DASYCU010000000
VERSION     DASYCU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3530046"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /locus_tag="VGM24_04490"
     CDS             <1..1813
                     /locus_tag="VGM24_04490"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEY4154655.1"
                     /translation="SPIALELAGGEVLGHHSVVRKASKVSVDKTFSTPFYKKSSVTDQ
                     YNELTLSFAGNYGLVFRAYNDGVAYRFTSHRRGELTIKNETASFHFASDHEGIFPLIQ
                     EYRVPRDPFMCSFESLYRQMPVSRFPKDTLAFLPALVNLGEGKRLAILEGDLEDYPGM
                     FLQPEKDSPNSLQGAFAGYPVKESLFENRLNARVDGRENFIARVEGKHEFPWRVLLLS
                     NSDKELLDNDMMQKLAPPSRVADISWIRPGKVAWDWWNDWNISRVNFKAGINTETYKY
                     YIDFAADNKLEYIILDEGWSNDTSLLEVSPAVNLDSIVAYGKNKNVGVILWSTMYAAA
                     KNTESTFAHYSRMGVKGFKIDFLDRDDQQMVRRVYEVADIAAKNKLVLDYHGMYKPSG
                     LQRTYPNVLNCEGVRGLENYKWSTVTSVPEYDAQIPFIRMLAGPMDYTPGAMRNASKA
                     SFRPINSMPMSLGTRCHQLAMYALYEAPLQMLSDNPVAYRQDSICTRFISLFPTVFDE
                     TVAIDGTVGEFAVVARRKGNSWYIGALNNWTARDLDIPLNFLEEGPYEAETFSDGLNA
                     ERDGTDYTRSVMPVQQQSSLHIHLAAGGGWAAVISKK"
     gene            1828..>2236
                     /locus_tag="VGM24_04495"
     CDS             1828..>2236
                     /locus_tag="VGM24_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154656.1"
                     /translation="MKRLVFYFLIFAFYIPLPVLSQQNQVRYWVTSGDRTQLLKEQPG
                     PLHFSQAKPNPDAIVVDASKKFQTIDGFGFALTGGSAQHIIQMDAADQKKLLREIFGK
                     NPGDISVSYLRISIGASDLNERVFTYDDMPEGQT"
BASE COUNT          546 a          545 c          605 g          540 t
ORIGIN      
        1 gtccccgatt gcgctggagc tggccggcgg tgaagtgctg ggtcatcact ccgttgtccg
       61 gaaagcttcc aaagtgagcg tggacaaaac cttttcaacg cctttctata aaaagtcctc
      121 cgttaccgac cagtacaatg agcttaccct gagctttgcc gggaattacg gcctggtttt
      181 ccgcgcctat aacgacgggg tcgcttaccg gttcacctcg catcgcaggg gagaactcac
      241 gataaagaac gagaccgcat cgtttcattt tgcatcggac catgaaggaa ttttcccgct
      301 tatccaggaa taccgtgttc cgcgagatcc ctttatgtgc agctttgaat ctctttaccg
      361 gcaaatgccg gtcagccgtt tcccgaaaga cacgctggcc tttcttccgg cgctggtcaa
      421 cctgggagaa ggaaaaagac tggctatcct cgaaggggat ctggaagact atccgggtat
      481 gttcttacaa ccggagaaag attctcccaa cagtttgcag ggtgcttttg ccggatatcc
      541 ggtgaaggag agtctgtttg aaaatagact aaatgcccgg gtggacggga gagaaaattt
      601 tattgcccgg gtggagggca aacatgagtt cccctggcgg gtgctgctcc tcagcaattc
      661 cgataaggaa ttgctcgaca acgatatgat gcaaaagctg gcccctccgt cccgcgttgc
      721 ggatatctcc tggatccggc ccggcaaagt ggcctgggat tggtggaacg actggaatat
      781 ttcgcgggtg aattttaaag ccggcatcaa cacggaaacc tataaatatt atatcgattt
      841 tgcagcggac aataaactgg aatatattat cctggatgaa gggtggagca atgacaccag
      901 tctgctggag gtttctccgg ccgttaatct ggacagcatt gttgcgtatg gcaaaaataa
      961 gaacgtgggg gtgatcctct ggtcgaccat gtatgcggca gcaaaaaata cggaatctac
     1021 ttttgcgcat tacagccgga tgggggtgaa aggatttaag attgattttt tggaccgcga
     1081 cgatcagcaa atggttcggc gcgtttatga agtggctgat atcgccgcaa aaaataagct
     1141 ggtcctggac tatcacggaa tgtataaacc atccggcctg cagcggactt atcccaatgt
     1201 gctgaactgc gaaggcgtgc gtggactgga aaattacaaa tggtcgaccg tgacctctgt
     1261 tccggaatat gatgcacaga ttccgtttat ccgcatgctg gccggaccga tggattatac
     1321 gcccggggcg atgcgcaacg ccagcaaagc cagcttccgg ccgatcaatt ccatgcccat
     1381 gagcctcgga acccgctgtc accaactcgc tatgtatgcg ttgtatgaag cgcctttgca
     1441 aatgctttcg gataacccgg tggcttaccg gcaggacagt atctgcaccc ggttcatttc
     1501 cctgttcccg acggtttttg atgaaaccgt cgccattgac ggaacagtgg gtgaatttgc
     1561 cgttgttgcc cggagaaaag ggaatagctg gtatatcggt gcgctgaata actggactgc
     1621 ccgggatctg gacattcccc tgaatttcct ggaagaagga ccgtatgaag cggaaacctt
     1681 cagtgatggc ctgaatgcgg aacgggacgg aacagattac acccgctcgg ttatgccggt
     1741 tcagcagcaa tcctcccttc acatacatct ggcggccggc ggcggctggg cggctgtgat
     1801 aagcaaaaaa taactaattc cggaaccatg aagcggcttg tattttactt tttaattttt
     1861 gcattttata ttccccttcc cgttctttcc cagcaaaacc aggtacggta ttgggtcact
     1921 tcgggcgacc ggacgcaatt attgaaggag cagccgggcc cgctgcattt ttcccaggct
     1981 aaacccaatc cggacgctat cgtggtggat gcttcaaaaa aatttcagac cattgacgga
     2041 tttgggtttg ccctgaccgg cggtagcgca cagcatatca ttcagatgga tgcggccgac
     2101 cagaaaaaat tactccggga aattttcggt aaaaatcccg gggacatttc ggtcagctat
     2161 ctccggatca gtattggggc ttcagacctg aatgaaaggg tgtttacgta tgatgacatg
     2221 ccggaggggc aaacag
//
LOCUS       DASYCU010000221         5529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_49801, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000221 DASYCU010000000
VERSION     DASYCU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49801"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGM24_04500"
     CDS             <1..243
                     /locus_tag="VGM24_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154657.1"
                     /translation="RRCQTCHSAGHENICKLTATLGNSINQNCIDCHMPKQKSRAIML
                     LLQGDKTPTSAVIRSHYIRIYPEETKKVLATMQFSK"
     gene            complement(269..1105)
                     /locus_tag="VGM24_04505"
     CDS             complement(269..1105)
                     /locus_tag="VGM24_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEY4154658.1"
                     /translation="MEIIIIVSLVVAVLILHRTLSELFNKFYIRRQQRKFIASETFYH
                     SIVSRYIRFYNKLSLEDQHKFLFRTFLFKKARKFHYIEVKESAEMPILISAVAVQLTF
                     GLEKFMLNYFNDIFVLKDDYHYGFYSRPFMGHVDQSGIYLSWDNFIKGISGLTPYCNV
                     GLHEMGHALAYVTFITETEEDKHFKKEFKNFSKVARPIFASMQAGNRNLLGEYAAVNY
                     HEFWAVSVETFFENPVCLRQDLPELYEAMSRLLKQDPLQVRNGKTIFLLKGANEKVES
                     LN"
     gene            1371..3197
                     /gene="aspS"
                     /locus_tag="VGM24_04510"
     CDS             1371..3197
                     /gene="aspS"
                     /locus_tag="VGM24_04510"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY4154659.1"
                     /translation="MYRTHDCGELRLVDVAREVTLSGWVQSLRKFGSISFVDLRDRYG
                     ITQLLFGEALNEKLAETPLGREYVIQVSGLVSERSNKNPQIPTGEIEIKVSRFTILNR
                     SATPPFTIQDDTDGGDDLRMKYRYLDLRRNEVKKNLELRYAVNRAARNYLHQHRFMDI
                     ETPFLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTFKQLLMISGYDRYYQVVKCFRD
                     EDLRADRQPEFTQIDCEMSFIEQDDILRMFEGLIKHIFKEVKGIDYTDAVERMSWEDA
                     MWNYGNDKPDIRFGMKIANLKSPLKRKGTQEADGLILSKADFNVFDQAETVVAISVPG
                     AAAWTRKQFDGLTEWVKRPQIGMKGLAYIRYNEDGSFKSSIDKFYSEERLKTIADFCE
                     AGKGDAILMLAGREERTRKAISELRLEMGEQLGLRNREEFNLLWVLDFPLFEYSEEDN
                     RWVARHHPFTAPKPDQISVMIDHSPAIPQPELYLEHPYAGIKANAYDMVLNGNEIGGG
                     SIRIFQRELQEKMFDALGMSREEANYKFGFLLGAFEYGAPPHGGIAFGFDRLCAILGG
                     SESIRDFIAFPKNNSGRDVMLDAPAGIDQKQLDELSISVKTS"
     gene            3275..4105
                     /locus_tag="VGM24_04515"
     CDS             3275..4105
                     /locus_tag="VGM24_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4154660.1"
                     /translation="MAYQLLKGKKGIIFGALDDKSLAWRTALRCYEEGAGLVLTNAPV
                     ALRLGEINKLAAAVQAPVIGADVTNMDDLKKLFESAMNHFGGKIDFILHSVGMSLNIR
                     KGKPYTGLDYGFNQKTLDISAMSFHRVLQTAYEMDAINNGGSVLALSYIAAQRVFPDY
                     NEMSDAKALLESVARNFGYYYGIRNKVRVNTVSQSPTKTTAGSGVKSFDGFQAYAEKM
                     SPLGNASADDCAGYCAVLFSDLTRMVTMQNLFHDGGFSFTGVTEAVVMEIEKNQEVLK
                     "
     gene            complement(4162..4383)
                     /locus_tag="VGM24_04520"
     CDS             complement(4162..4383)
                     /locus_tag="VGM24_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEY4154661.1"
                     /translation="MFWTLELASYLEDAPWPATKDELIDYAIRSGAPIEVVENLQELE
                     DEGEIYEGIDDIWPDYPSQEDFFFNEDEY"
     gene            4569..5249
                     /locus_tag="VGM24_04525"
     CDS             4569..5249
                     /locus_tag="VGM24_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4154662.1"
                     /translation="MIPAAATQPILSARNIEKYYGELRVLKGVSLDINKGEIVSMVGP
                     SGSGKSTLLHILGTLDLPRSGEIILESRPLHTLKGKPLAAFRNRHIGFVFQFHHLLPE
                     FTALENVCIPGWIAGRKKSEVRAGAIQLLNILGVGHRTEHKPAALSGGEQQRVAVCRA
                     LINHPDIVFADEPTGNLDSANAQELHRLFFDLRRQFNQTFLIVTHNEALAEQSDRVLH
                     MKDGSLIP"
     gene            complement(5250..>5529)
                     /locus_tag="VGM24_04530"
     CDS             complement(5250..>5529)
                     /locus_tag="VGM24_04530"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HEY4154663.1"
                     /translation="LEKEIDRMSGSLPPLKFFILPGGHVVVSTAHICRCVCRRAERSA
                     VHLDEAESPVQPLIIKYLNRLSDYLFVLSRYAGHQLGAAEIPWVARRG"
BASE COUNT         1416 a         1326 c         1446 g         1341 t
ORIGIN      
        1 cggaggtgcc agacctgcca tagtgccggc catgaaaaca tctgtaagct gacggccacc
       61 ctgggtaatt ccatcaatca gaattgtatc gattgtcaca tgcccaaaca gaaatcccgc
      121 gccatcatgt tgttgttgca gggagataaa acacccactt cggctgttat ccgttcccat
      181 tatatccgga tatacccgga agaaacgaaa aaggtcctgg ctacgatgca gttcagtaaa
      241 taagccgggc tatcgggagg gagagatctt aattcaggct ttccactttc tcgttagctc
      301 cttttagcag gaaaatggtt ttcccgttcc ggacctgtaa ggggtcctgt tttaacagtc
      361 tgctcatcgc ttcgtagagt tccggaaggt cctgtctcag gcataccggg ttttcgaaaa
      421 aggtttcgac ggagacggcc cagaattcgt ggtaattgac ggccgcatat tcacccagca
      481 gattgcggtt accggcctgc atactggcaa agatcgggcg ggccactttg gaaaagtttt
      541 taaattcctt cttgaaatgc ttgtcttctt ctgtttcggt aataaaggtc acataggcca
      601 gcgcatggcc catttcgtgc aggcccacgt tgcaataggg ggttagtccg ctgatcccct
      661 taatgaaatt atcccaggaa agatagatgc cgctctgatc cacatgtccc atgaaggggc
      721 gggaataaaa cccgtagtgg taatcatcct tgagtacgaa aatatcgttg aaataattca
      781 gcatgaattt ttccaggcca aaggtgagct ggacggccac cgcgctgatg aggatgggca
      841 tttctgcact ctccttcacc tcgatataat ggaatttccg ggccttttta aaaagaaatg
      901 tgcggaacag gaacttgtgc tggtcttcca ggctcagttt gttataaaac cggatgtacc
      961 gggaaacaat gctatggtaa aaggtctcgc tggcaatgaa tttccgctgc tgtctgcgga
     1021 tataaaactt attgaaaagt tcagaaagtg tccggtgcag tatcaataca gccaccacca
     1081 gggaaacaat gataatgatc tccataaggt ccgtttttct atttgcttgc ttcaggttgg
     1141 tccgatcaga gtcttggcct tacatgggtt ggatagggag ggattcccgc ggttaatagg
     1201 aaagtcctat taaagtttca tcatgaggag aatgaataat acggatgcta caaggtaaaa
     1261 actatttttt ataaatgaaa gtgggcgagc cgggtttttt aatttttttt taaaccttcc
     1321 ggctctacct gccattgccc tacctttgcc ggctgaatca agcgataact atgtatagaa
     1381 cacatgactg tggcgagtta cggctggttg atgttgcccg ggaagtgacc ctgtccgggt
     1441 gggtgcaaag cctcaggaag tttggcagca tcagttttgt cgaccttcgt gaccgctacg
     1501 ggatcaccca attgcttttt ggggaggcat tgaatgaaaa gctggcagag acgcctttgg
     1561 gccgggaata tgttatccag gtttccggcc tggtgtccga gcggtccaac aagaatccac
     1621 aaatccccac cggggaaatt gagataaaag tgagccggtt tactattttg aacagatccg
     1681 ccaccccgcc atttaccatc caggacgata cggatggggg agatgatctg cggatgaaat
     1741 accgctatct cgacctgcgc cgcaacgaag tgaaaaagaa cctggaattg agatatgcgg
     1801 ttaaccgggc cgcccggaat tatcttcacc agcaccggtt catggatata gaaaccccct
     1861 tcctgatcaa atccacgccc gaaggtgccc gggattttgt tgttccgtcc aggatgaatc
     1921 cgggccagtt ttatgctctg ccgcagagcc cgcagacctt taaacaactg ctgatgatca
     1981 gcggctacga ccggtattac caggtggtga aatgcttccg cgatgaagac ctgagagccg
     2041 accgtcagcc ggaattcacc cagatcgact gcgaaatgag ttttattgaa caggatgaca
     2101 tcctccggat gttcgaaggg ctcatcaagc atattttcaa ggaagtaaaa gggatcgatt
     2161 atacggatgc cgtggaaagg atgagctggg aagatgccat gtggaactat gggaatgaca
     2221 aacccgatat ccggttcgga atgaaaatcg ccaacctgaa gtctcctttg aaaaggaaag
     2281 gaacgcagga agcagacgga ttgatcctca gcaaagcgga ttttaatgta tttgaccagg
     2341 ccgagacggt ggttgcgatc tcggttcccg gcgcagccgc ctggacacgc aaacagtttg
     2401 acgggctcac cgaatgggtg aagcgtccgc agatcggtat gaagggactg gcgtatatcc
     2461 ggtataatga agacgggtca ttcaaaagca gcatcgataa attttattcc gaagaacggc
     2521 taaaaaccat tgccgacttt tgcgaagccg ggaaaggaga tgcgatcctg atgctggccg
     2581 gccgggagga aaggacccgc aaggccatca gcgaactccg cctcgaaatg ggtgaacagc
     2641 ttggactccg gaatagggaa gagttcaact tgttgtgggt cctggatttt cctctgtttg
     2701 aatacagcga agaggacaac cggtgggtgg cgcgccatca tccctttaca gcgccaaagc
     2761 ccgaccagat atccgtcatg atcgaccatt caccggctat tccgcaaccc gaactatacc
     2821 tggagcatcc atacgccggt atcaaggcca atgcgtatga tatggtgctg aacgggaatg
     2881 agatcggggg cgggtctatc cgtattttcc aacgggaact gcaggaaaaa atgttcgacg
     2941 ccttaggcat gagccgcgaa gaggccaatt ataaattcgg ttttctgctc ggcgcttttg
     3001 aatacggcgc accgcctcac ggggggatcg cctttgggtt cgaccggctt tgcgctatcc
     3061 tggggggaag cgaaagcatc cgcgatttta ttgctttccc gaaaaacaac agcggacggg
     3121 atgtgatgct cgatgcgcct gccggcattg atcagaagca actggacgag ctttctattt
     3181 ccgtcaaaac atcataggcc ggccctgatc cgatgaagag gccgctgatg aaaatatctt
     3241 cttattttta cctacataaa atttatccgg acacatggcc tatcaactgc taaaaggaaa
     3301 aaaaggaatt attttcggtg cactcgacga taagtccctc gcctggagaa cggcattgcg
     3361 ttgttacgaa gaaggcgccg ggctggtttt gaccaacgcg ccggttgccc tgcgtttggg
     3421 agagatcaat aaattggcag cggccgtgca ggctccggtc atcggggccg atgttaccaa
     3481 tatggacgac ctgaaaaagc tttttgagag cgccatgaac catttcggcg gaaagattga
     3541 ttttatactc cattccgttg gcatgagcct caacatcaga aaggggaagc cctatacggg
     3601 gctcgattac gggtttaacc agaaaaccct ggatatctct gccatgtctt ttcaccgcgt
     3661 tcttcaaacc gcgtacgaaa tggatgccat caataacggc ggaagtgtac tggcactaag
     3721 ttatattgcc gcccaacggg tgtttcccga ttataatgaa atgtcggatg ccaaggcgct
     3781 gcttgaaagt gtggcgcgga atttcggtta ttattatggt atccggaata aggtccgggt
     3841 gaataccgta tcccagtctc ccaccaagac cactgccggt agcggggtga aaagctttga
     3901 cggtttccag gcctatgcgg aaaaaatgag cccgctggga aatgccagtg cggatgattg
     3961 cgcgggctat tgtgccgttt tattcagcga cctgacgcgc atggttacga tgcaaaacct
     4021 ttttcacgac gggggatttt cctttaccgg cgttaccgaa gcggtggtta tggaaattga
     4081 gaagaaccag gaagtgctga agtagataaa aataaaacag gaagttcggt gacttcctgt
     4141 tcctgttcaa atcctgattt tttaatattc atcttcatta aagaagaaat cttcctgcga
     4201 agggtaatcc ggccagatat cgtcaatccc ctcataaatc tccccttcat cttccagttc
     4261 ctgcaggttt tcaaccactt cgatcggggc gccggaacgg atcgcataat cgatcaattc
     4321 gtctttggtt gcgggccagg gggcgtcttc aaggtagctg gcgagttcga gtgtccaaaa
     4381 catctttatt ccggtattta atttctgtat gttctcaata aggtcagatc aacgctattt
     4441 cccggagaat gttgggaaac gctgccttta atttttcgca aaagtaattt ttataatgaa
     4501 accaccaaat accatttata gggcagatcc ggggtattcc gcattagcca agcttgttat
     4561 atttgctgat gatcccagca gcagccaccc agcccattct ttccgcgaga aatatcgaaa
     4621 agtattacgg ggaattgcgg gttttaaaag gggttagcct ggatattaac aaaggggaaa
     4681 tcgtaagtat ggtgggaccg tccggatcgg gcaaaagcac cctcttacat atcctgggaa
     4741 cattagattt accccggtcc ggggagatca tcctggaaag ccgtcctttg cataccctga
     4801 aagggaaacc cctggccgcc ttccggaaca ggcatatcgg ttttgttttt cagttccatc
     4861 acctgctgcc ggaattcacc gccctggaga atgtgtgcat accgggctgg atcgccggcc
     4921 ggaaaaaatc ggaagtcaga gccggggcca ttcagttgct gaatatcctg ggcgtaggcc
     4981 accgcacaga acataaaccg gctgccctct ccggtggcga acaacagcgg gtggctgtgt
     5041 gccgcgccct gatcaaccat ccggatattg tgtttgccga tgaacctacc ggtaacctgg
     5101 attctgccaa tgcccaggaa ctacaccggt tattttttga tctgcgccgg cagttcaatc
     5161 agaccttcct gatcgtaacc cacaatgaag cgctggcgga gcaaagcgac cgggtgctac
     5221 atatgaaaga tggaagtcta atcccctgat taacctcgtc ttgcgaccca ggggatctcc
     5281 gctgctccca gctgatgccc ggcataccgg gaaagcacaa agaggtagtc actcaggcgg
     5341 ttgaggtatt tgataatcag cggctgaacc ggggattccg cttcatccag atgaacggcg
     5401 ctgcgttcgg cgcggcggca aacacagcgg caaatatgcg cagtggaaac caccacatga
     5461 ccgcccggca gaataaagaa tttcaggggc ggcagggatc cgctcatccg gtcaatttct
     5521 ttttccagc
//
LOCUS       DASYCU010000222         3239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1208322, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000222 DASYCU010000000
VERSION     DASYCU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208322"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGM24_04535"
     CDS             complement(<1..344)
                     /locus_tag="VGM24_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY4154664.1"
                     /translation="MPNNSVSLHRVLKASPEKVYRAFTEANAIASWLPPYGFLCTVHE
                     MKAEVNGTYKMSFQNFTTGNSHSFGGKYLEIKPNAFLKYTDKFDDPNLPGEMTTTVSL
                     QKVSVGTEIKIS"
     gene            complement(449..2032)
                     /locus_tag="VGM24_04540"
     CDS             complement(449..2032)
                     /locus_tag="VGM24_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEY4154665.1"
                     /translation="MHSILPFLVAMIAAVVLLQMWAAKLKIAYPIMLVVAGLLVGFIP
                     GLPAVRVHPDLIFFIFLPPLLFEAAWTISFKEMKKWWRIIGSFAFLVVFFTALSVALV
                     TNHFIPGFSIALGFLLGGIVSPPDAVSTGAITKFVKIPRSTSAILEGESLLNDASSLI
                     IFRFALVAVGTGQFVWQQAAATFLWMVIGGAGIGLLLAWIFVQAHKRLPTDAPSDIAL
                     TLIEPYIMYWVAEELNSSGVLAVVSGGLYMSVRRLFFLNSTSRIRGYSVWESFVFILN
                     GIVFLLIGLELPEIVHGMRSNGFPLRTGIGYGLLVTAVLILARIISSYVALLATLLFR
                     PGVIPRTSSRRRRLLLPLTLGWTGMRGVVSLAAALAIPVTLNDGSAFPYRNLILFITF
                     VVILVTLLVQGLTLPYLIKRFRLFEEFGGQEPEEKMRQKMKHGLKESIRQFLREKYEN
                     GKEGDTDMQKMLKHWEGKSKTGDDDPINEKTKAVFLEVLEKQREYLENLNKDPSITEE
                     IIRTQLYQIDLEEERLKML"
     gene            complement(2053..>3239)
                     /locus_tag="VGM24_04545"
     CDS             complement(2053..>3239)
                     /locus_tag="VGM24_04545"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY4154666.1"
                     /translation="PLLLLFPAINFAQPGPKADGIVQSIRRIDGSSIAAGQLDQQVQK
                     LMEDAKVTGLCITVFNNNQPVFSKTYGYAIAATRQPMTSSTALYGLSLSKAVFTYLVM
                     QLVDEKKIELDKPLVNYLPRSLMTYTFPGRLSSYKDLEGDDRYKKITARMCLDHTTGF
                     PNFRWFEPDRKLRIKFEPGSRVSYSGEGMYFLQMIIQEITGKNLEELARQYIFEPCGM
                     TRSSYVWQPGYETDYAVGHDSKNDSTFFPRRPLANAAGTLTTTMDDFNRFYTMLINGK
                     RLSEKSFNEMTHSQIRIRSIKQFGPLSWKDSTLNDNIRLGYGLGYGVLQSPYGRAFFK
                     EGHDDGWGHYTIAYPDKKIAVVILTNSDNGEGIFKDLLREAIGDIYTPWYWENYIPWN
                     ER"
BASE COUNT          805 a          820 c          830 g          784 t
ORIGIN      
        1 tgtgagatct ttatttccgt gccgacggaa actttctgca gcgagaccgt ggtggtcatt
       61 tcgccgggaa ggttggggtc gtcaaattta tcggtgtatt ttaggaacgc attgggtttg
      121 atctcgagat attttccccc gaaggagtga ctgttaccgg tggtgaaatt ctgaaatgac
      181 attttatagg taccgttcac ctccgctttc atttcatgaa cggtgcacag aaaaccatag
      241 ggaggcaacc aggatgctat ggcattagcc tcggtaaatg cccggtaaac tttttccggg
      301 gaagctttca gcactctgtg caaggaaaca ctgttgttcg gcataagatt ttatttaaat
      361 gattttcgac cggctgcaat ggcagcggat aaagttatcc ttcgaacatt caaaaatgat
      421 gcctgccata tcgaacgtta acagactttt acagcatttt caggcgttct tcctccaggt
      481 cgatctggta taactgggtg cggattattt cttcggtaat cgatgggtcc ttattgaggt
      541 tttccaggta ttcccgctgt ttttcgagca cctccaggaa gacggccttg gttttttcat
      601 ttatcgggtc gtcatccccg gtttttgatt ttccttccca gtgctttaac attttttgca
      661 tatcggtatc gccctctttt ccgttttcat atttttctct cagaaattga cggatagact
      721 ctttgagccc gtgtttcatt ttttgcctca tcttctcctc cggctcctgc cccccgaatt
      781 cctcaaacag ccggaagcgt ttgatcaaat agggcagtgt gagtccctgt accagcaaag
      841 tgaccagtat cactacaaag gtgataaaca ggataaggtt tctataggga aaagcgctcc
      901 catcgttcag cgtcacggga atagccaggg cagcagccag cgacaccact ccgcgcatgc
      961 ccgtccaacc cagggtcagc ggcaacagga gtcgccttct cctggagctg gtgcgcggaa
     1021 tcacgcccgg ccggaaaagg agtgtagcga gcaaagcaac ataagagctg atgatccggg
     1081 caaggatcag cacggccgtg accagcaaac cgtagccgat gccggtacgc agcgggaatc
     1141 cgtttgaacg cattccatgc acaatttccg gcagctccag cccgatcagc aggaacacga
     1201 tgccgttcag gatgaacacg aaactttccc atacgctata gccccggatg cggctggtgc
     1261 tgttcaaaaa gaaaagccgc cgcacggaca tatacaagcc cccgctgacc acggccagca
     1321 cgccggagct gttcagctcc tccgccaccc aatacatgat ataaggttcg ataagcgtaa
     1381 gcgcaatatc cgacggcgca tcggtgggaa ggcgtttatg cgcctgtaca aatatccacg
     1441 ccagcaataa gccgatgccg gcgccgccga tcaccatcca aaggaaagtg gctgcggcct
     1501 gctgccacac gaactggccc gtacccaccg cgaccagggc aaagcggaat atgatcagtg
     1561 aagaagcgtc gttgagcagg ctttctccct ccaggatggc cgaagtggac ctggggatct
     1621 ttacaaactt ggtgatcgcg ccggtgctca ccgcatcggg cggcgaaaca ataccgccca
     1681 gcagaaatcc cagggcaatc gaaaaccccg gaataaaatg attggtgacc agtgccacgg
     1741 ataaagccgt aaaaaagacg accaggaagg caaagctgcc gatgatgcgc caccattttt
     1801 tcatctcttt gaaagaaatg gtccaggcgg cttcaaacag gagcggcggc aggaaaataa
     1861 aaaaaattag atccggatgg acccgaacgg caggcaatcc cggaataaag cccaccagga
     1921 gcccggccac caccagcata atgggataag cgatcttaag tttggcagcc cacatctgca
     1981 gcaggaccac cgcggctatc atggcaacca ggaatggcag tatgctgtgc atggcgcgga
     2041 catttaaaag atttaacgct cgttccaggg tatataattt tcccaatacc agggggtata
     2101 tatatcacca atagcctccc ggagcagatc tttaaaaatg ccttccccat tgtctgaatt
     2161 ggtgagtatt accacggcaa tttttttgtc ggggtacgca atggtatagt gtccccagcc
     2221 atcatcgtgc ccctctttga aaaaggcccg gccataagga gattgcagca ccccataccc
     2281 aagtccatag ccgaggcgga tattgtcatt tagtgttgaa tctttccagg aaagaggccc
     2341 gaattgtttg atggaccgga tacggatctg cgaatgcgtc atctcgttga aggatttctc
     2401 tgataatcgt ttgccattga tcagcatcgt ataaaagcga ttgaaatcat ccatggtcgt
     2461 ggtaagggtg ccggctgcgt tcgccagcgg tcgccgggga aagaaagtac tgtcattttt
     2521 gctgtcgtgg cctacggcat agtccgtttc atatccgggt tgccaaacat aacttgaccg
     2581 ggtcataccg cagggttcaa aaatgtattg tctggccagt tcttccaaat tctttccggt
     2641 gatttcctgg atgatcatct gcaggaaata cattccttcc cccgaatagc ttacgcggct
     2701 gcccggttca aacttgatgc gcaatttcct gtcgggttca aaccagcgga agttcggaaa
     2761 accggtcgta tgatcgaggc acatgcgggc cgtgatcttt ttatagcggt cgtctccctc
     2821 gagatcttta tatgagctaa gtctgcccgg gaaggtatag gtcatcagcg accggggcag
     2881 atagtttacc agcggtttgt cgagttcgat ctttttttca tccaccaatt gcatgaccag
     2941 gtaagtaaaa acggccttgc tgagcgacag cccatataaa gcggtagacg aagtcattgg
     3001 ctgccgggtg gcggcgatag catagccgta ggttttggaa aataccggtt ggttgttatt
     3061 gaagacggta atgcaaagtc cggtcacctt tgcatcttcc atcaactttt gtacctgctg
     3121 atcaagctgt ccggctgcaa tggaggaacc atcaatccgc cggatggatt ggacaatgcc
     3181 atccgctttg gggccgggtt gagcgaagtt tatagccggg aagagcagca gtaacggaa
//
LOCUS       DASYCU010000223         3867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_338633, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000223 DASYCU010000000
VERSION     DASYCU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338633"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGM24_04550"
     CDS             <1..291
                     /locus_tag="VGM24_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEY4154667.1"
                     /translation="YATGEQWDAPNGWAPLEWMMIWGLDRCGQKNLAATAAKRWIRLN
                     TRVYQKTGKLMEKYNVVDMTKVAGGGEYGGQDGFGWTNGVLLKLISMYGKGN"
     gene            312..779
                     /locus_tag="VGM24_04555"
     CDS             312..779
                     /locus_tag="VGM24_04555"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY4154668.1"
                     /translation="MTDEYFMLQALKEAKKAYSEGEVPVGVVITMENRIIARGHNQVE
                     RLNDPTAHAEIIALTSAFNLIGSKYLPEACIYITIEPCLMCAGALYWSKIRKVVYGGR
                     DEKNGYQKYIIPCPSLEQLGVAPPPFHPKTIVASGVLKEECSILMKEFFRERR"
     gene            889..1494
                     /locus_tag="VGM24_04560"
     CDS             889..1494
                     /locus_tag="VGM24_04560"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY4154669.1"
                     /translation="MAFTLPALPYAPAALEPHIDALTMQIHHDIHHQAYVDNLNKAVA
                     GTPNENKSLEDLVKVAGTLSPAIRNNGGGHWNHSFFWEILAPNAGGKPSGKLSESIDA
                     DFGSFDSFKEKFAQAGAGRFGSGWTWLVEQDGKLVISSTPNQDNPLMDVAEVKGTPVL
                     GVDVWEHAYYLKYQNRRAEYLTAFWNVVNWKEVSRKYEASL"
     gene            1573..2181
                     /locus_tag="VGM24_04565"
     CDS             1573..2181
                     /locus_tag="VGM24_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154670.1"
                     /translation="MKKSFALLLSIAFLFSACKSHNKKILVYANSNIQVDDSQKNIIV
                     KDGTTHVEKELNFSGSDPVVLNIQGPTGKYTLEAKEDGLYIANLKTDTVVGSLQHTGE
                     TAHTRLTQDQLKANLDSLNKLIRDENVSATSKNYYIAPGKMVRLSEFTKAKVFGPFTS
                     IPSSFDAGSVPEIYKFYNLSEVRDIIAKLTEMSKYKEDGEEK"
     gene            complement(2204..2434)
                     /gene="yidD"
                     /locus_tag="VGM24_04570"
     CDS             complement(2204..2434)
                     /gene="yidD"
                     /locus_tag="VGM24_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY4154671.1"
                     /translation="MKEILQILSLPLIGLIKLYQWVISPWLGPKCRFTPSCSQYAIEA
                     FRKYGLFKGGWMAIRRISRCQPWGGSGHDPVP"
     gene            complement(2385..2816)
                     /gene="rnpA"
                     /locus_tag="VGM24_04575"
     CDS             complement(2385..2816)
                     /gene="rnpA"
                     /locus_tag="VGM24_04575"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY4154672.1"
                     /translation="MAGIRINTLGKADRLKSRKQIDGLFESGKKIMIFPFRVMYKMAP
                     GGCGLKAGFTVSSKNFPRSVDRNRIKRLTREAYRLQKNPLRELVQKNRQQVALFLIYT
                     GRELPEFPAVQKSIQQVLEKLVSQLHEGNSSNTQFAPDRVD"
     gene            complement(2886..3041)
                     /gene="rpmH"
                     /locus_tag="VGM24_04580"
     CDS             complement(2886..3041)
                     /gene="rpmH"
                     /locus_tag="VGM24_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY4154673.1"
                     /translation="MKRTFQPHTRRRKTVHGFRKRMQSANGRKVLASRRAKGRAKLTV
                     SDEHKAK"
     gene            complement(3182..>3867)
                     /locus_tag="VGM24_04585"
     CDS             complement(3182..>3867)
                     /locus_tag="VGM24_04585"
                     /inference="COORDINATES: protein motif:HMM:NF039704.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HEY4154674.1"
                     /translation="PGFQTGVYSVNAQSEGFAFFSNIQDTTNLYLSNSSPDVSKAGGT
                     VSLTVVDTVNKTISGTFQFNLYQASSGSTKTITSGVFNNIPYTGSGGTTLPPIQAPPG
                     SNTDTLYAQVDSVAWEAAQVLVNAQSGSLIIGGISSDAQQTLGLYMPANITAGTYSLD
                     FSSGIYFATFSPGPTSSLFSIGTGNSLTILENDTVNRRIKGNFNFVGKSQTDSSSATI
                     TNGYFSAAY"
BASE COUNT         1051 a          922 c          954 g          940 t
ORIGIN      
        1 tatgccaccg gtgaacagtg ggatgcgccg aacggatggg ctccgctgga atggatgatg
       61 atctgggggc tggatcgctg cgggcagaaa aacctggcgg caactgcggc aaagagatgg
      121 atccgcttaa atacccgggt ataccaaaaa accggtaaac tcatggagaa atataatgtg
      181 gtggatatga ccaaagtggc cggaggtggg gaatatggtg gacaggatgg gtttggctgg
      241 accaacggcg tactgctcaa actgatcagc atgtacggaa aaggaaattg atcacaaaat
      301 ttaaaggtat aatgaccgac gaatatttca tgttacaggc attaaaagaa gccaaaaaag
      361 cttattcgga gggggaagtc ccggtcggtg tcgtgatcac catggaaaac aggatcattg
      421 cccgtggtca taaccaggtg gaacggttga atgaccccac cgcgcatgcc gaaatcatcg
      481 cgctgacatc cgcattcaat ctgatcggct caaaatatct gccggaggca tgcatttata
      541 ttaccatcga accctgcctg atgtgtgccg gagcccttta ctggagtaag atccggaaag
      601 tggtatatgg cggccgggac gaaaaaaatg gttatcagaa atatataatt ccctgccctt
      661 cactggaaca actgggcgtt gccccgcctc cctttcatcc aaaaaccatt gtcgcatccg
      721 gggtacttaa agaagaatgc agcattttaa tgaaggagtt ttttagggaa agaaggtgaa
      781 cacattccac gatcagcctg gtattagttt tgaggaatca actattgatc cctaattttg
      841 ttgtcccaag aagaacagca ctactcaaat tcattaaaaa caatcgttat ggcatttaca
      901 ttacctgcac ttccttacgc tccggctgcg cttgagccgc atatagatgc gttgaccatg
      961 cagattcatc acgacataca tcatcaggct tatgtagaca atctgaataa ggcagttgcc
     1021 ggcacaccca acgagaacaa gtccctggag gatctggtaa aagttgccgg gacgctttct
     1081 cccgccattc gcaacaatgg gggtggacac tggaaccata gtttcttctg ggagatcctg
     1141 gcgcccaatg caggcggaaa accttccggc aagctctccg aatccatcga tgccgatttc
     1201 ggatcctttg attcctttaa ggaaaaattt gcccaggccg gggccggccg ctttggaagc
     1261 ggttggacat ggctggtgga gcaggacggg aaactggtga tctcctcaac acccaaccag
     1321 gataatcccc tgatggatgt ggctgaggtt aaagggacgc cggtactggg cgtggacgtt
     1381 tgggaacacg cttattatct gaaataccag aatcgccggg cagaatacct gaccgccttc
     1441 tggaatgtgg ttaactggaa ggaagtcagc aggaaatatg aagcatccct ctaactatta
     1501 aattaacaat ttttttatcc acaacaatcc cggtacctct tttaccggga ttcctttttt
     1561 aaatgactct ttatgaaaaa atcctttgca ctccttcttt ccatagcctt tcttttcagc
     1621 gcctgtaaat cacataataa aaagatcctg gtttatgcca atagtaatat ccaggtggat
     1681 gattcgcaga aaaacattat tgttaaggac ggcaccaccc acgtggaaaa agagttgaat
     1741 ttttccggca gtgacccggt cgtactgaat attcagggac ccacggggaa atataccctg
     1801 gaggcgaaag aggatggtct ctatatcgcg aatctgaaaa ccgataccgt tgtgggaagc
     1861 ctgcagcaca ccggcgaaac ggcacacacc cgcctgacgc aggaccagct gaaagcaaac
     1921 ctggacagcc tgaacaaact gatccgggat gaaaatgttt ctgccaccag caaaaattat
     1981 tatatcgctc cgggaaaaat ggtgaggctg tcggagttta ccaaggccaa agttttcgga
     2041 cccttcacct cgatcccctc ttcctttgac gccggatccg taccggaaat atataagttt
     2101 tacaatcttt ccgaggtgcg tgatattatt gccaaactaa cggagatgag taagtataag
     2161 gaagacgggg aagaaaaata aaaagtaagg gaccggatta aaatcacgga accggatcat
     2221 gcccgcttcc tccccagggc tggcacctgg aaattctcct tatcgccatc cagcctcctt
     2281 taaacaaacc gtatttccgg aacgcctcaa tggcatattg agaacaactg ggggtgaacc
     2341 ggcacttcgg tcccagccag ggagaaataa cccattgata aagtttaatc aacccgatca
     2401 ggggcaaact gagtatttga agaatttcct tcatgcaatt gactaaccag tttttccaga
     2461 acctgctgaa tgcttttctg aacggccgga aattcaggca attccctgcc ggtataaata
     2521 agaaacaaag caacctgctg cctgttcttt tgaacaagtt cccggagcgg gtttttttgc
     2581 aaacgatagg cttcccgggt gagccgtttg atccggttcc tgtccacgga cctcggaaaa
     2641 ttttttgaac ttaccgtaaa ccctgctttc agcccgcatc cccccggggc cattttatac
     2701 atgacccgaa acgggaagat cataattttt tttccacttt cgaaaagccc gtcaatttgc
     2761 ttgcggcttt tcagacgatc cgctttaccc aatgtgttga tcctgatccc tgccatgatg
     2821 aatacaaaaa taaacagccc cggtttgccg gggctaaaag atatcgggaa ctttccggtt
     2881 atgaattatt tcgctttatg ttcgtcggaa accgtcagct tggcgcggcc cttggcacgg
     2941 cggcttgcca gcacttttct gccgttggcc gattgcatac gtttgcggaa gccgtgaacg
     3001 gttttgcggc gacgggtgtg gggttgaaat gtacgtttca ttgtttaaaa attttatcct
     3061 gcataattac ctgaaacaca ggtagttttc cattcatttc ccgaacaggc cgggctgccc
     3121 tgctgaaagg gaggcaaagg taaataattc ctgtttgatc gccaacaatc cacggagtta
     3181 atcagtacgc tgccgaaaaa tacccattgg ttatggttgc cgaactgctg tcggtctgcg
     3241 attttcccac aaaattgaaa ttccccttga tacgccggtt cacggtatcg ttttcgagga
     3301 tggtgaggct gtttccggtc ccaatggaaa aaagggaact ggtcggaccc gggctaaagg
     3361 tggcaaaata aatcccggaa ctgaaatcca gggaataggt tcccgcagta atgttcgccg
     3421 gcatatacaa accaagcgtc tgttgggcat cggaggagat cccgccgatt atgagcgacc
     3481 cggattgcgc attcaccagg acctgtgcag cctcccaggc gacactgtcc acctgggcgt
     3541 aaagggtatc cgtatttgac ccaggaggcg cctgaatggg gggcagcgta gtgccgcccg
     3601 agccggtata gggaatattg ttaaaaaccc ccgaggtgat cgttttggta ctgccactcg
     3661 atgcctggta aagattgaat tgaaacgtcc cggaaatggt cttatttacc gtatccacaa
     3721 cggtcaggct taccgttccc ccggctttcg atacatcggg gctcgaattg ctgaggtaaa
     3781 gattcgtggt atcctgaata ttcgaaaaaa aggcaaatcc ctcagattgg gcattgactg
     3841 aataaacacc ggtttgaaat ccgggta
//
LOCUS       DASYCU010000224         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4690098, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000224 DASYCU010000000
VERSION     DASYCU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4690098"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VGM24_04590"
     CDS             <1..868
                     /locus_tag="VGM24_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4154675.1"
                     /translation="EAGEWMGDDSRISLVSATGSTRMGKALGAQVAGRMGKSLLELGG
                     NNAIIISEHADLDIAVLGSLFGAVGTAGQRCTSTRRLIIQEKIYEQVKNRLVAAYRQL
                     RIGDPLDEKNHVGPLIDRAATAMYLNAIEECKKEGGKFIVEGGLLSGKGYESGCYVRP
                     CIAEAEPGFKTVQTETFAPILYLLKYKTLEEAIAIQNDVPQGLSSSIMTLNLREAEQF
                     LSAAGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKSYMRRQTNTINYST
                     QLPLAQGIKFDL"
     gene            979..2445
                     /locus_tag="VGM24_04595"
     CDS             979..2445
                     /locus_tag="VGM24_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4154676.1"
                     /translation="MNFTRISTRFSILFLFLHFSHSTAAQIRLAAVAGVHSATFQETN
                     SLPGFDSTQGRFTSSKTGVQLGVLAEIPLGNKNFFLQPGMNYSSKGSQYERFYDTLSG
                     QKDTLYNQYTLKLNYIEVPLYLTYKFPLSKNKPNYFYLGAGPYFAFIYNASKPYQNRV
                     KYYGNNDYTYNSGTQDLEVGNGPGKYKTLDFGINAKAGFELGNVLLGAYFSRGLTNAY
                     EASYPSSFHHQLIGASLGIWLNKIKPVPPPNTDSDHDGTPDRDDSCKTIPGSPRYHGC
                     PVPDTDRDGVNDEADSCKNVPGLARYHGCPIPDTDGDGVNDEEDSCKTIPGTAKYHGC
                     PIPDTDGDGVNDEADHCPDQAGLPENNGCPVVPVKLAKRILFVAKNVQFRSNSSKLTP
                     NSLPALRELADTLKANPDLELVVEGHTDNSGSPEYNQTLSEQRAAEVKKALIKLGVPE
                     ELISTRGYGDTQPSGDNRTREGKSKNRRVVFRLHVKNS"
     gene            2549..>3826
                     /locus_tag="VGM24_04600"
     CDS             2549..>3826
                     /locus_tag="VGM24_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY4154677.1"
                     /translation="MIRLKQTMLALFLLTVSLRPFAQGTSSRTELPKGWHLLDEEKDG
                     VYGISLQKAYDFLHARNLKSKTVLVAVIDSGVDTLQEDLSHILWVNPGEIPGNGIDDD
                     HNGYIDDVHGWNFIGGKDGSNVKDDSQEEGRVYYRYKDQFDNKDIRPDSLNATDRDHY
                     DMWLIAKKKIMGDGTESGIDLLMLKRVVNACIKSDSILQKAMGKTEYTGNDVDSFQAT
                     DVDARTAKSVLLFLFKENHIMDMTNTTFLTDFKEQADREEKKMEIKETPPRNYRAEIV
                     KDNENDINDSIYGNNDIMAGDPMHGTHVSGIIAAERNNNKGMNGIADNVRIMMIRAVP
                     NGDEHDKDIALAIRYAVDNGAKVVNMSFGKNLSPEKSWVDQAVEYAESKGVLLIHAAG
                     NDAEDVDSVGNFPNPVIKDSNKRADNWITVGASS"
BASE COUNT         1059 a          918 c         1023 g          826 t
ORIGIN      
        1 ggaagccggc gaatggatgg gtgacgatag ccggataagc ctggtttcag ccaccggttc
       61 cacccgcatg gggaaagccc tcggcgccca ggtggccggc cggatgggaa aatccctgct
      121 cgaactgggc ggcaataacg ctattatcat cagcgaacac gccgatctcg atattgctgt
      181 tttgggttcc ttgttcggag cggtgggaac ggccgggcag cgctgtacca gcacccggcg
      241 cctgatcatc caggaaaaaa tatatgaaca ggtgaaaaac aggctggtcg cggcttaccg
      301 gcaactccgc atcggcgatc cgctggatga aaagaatcat gtaggaccgt tgattgaccg
      361 cgccgccaca gcgatgtacc tcaatgcgat agaagaatgt aagaaagagg gtgggaaatt
      421 catagtggaa ggcggtcttt tatccggcaa gggatatgaa agcggctgtt atgtaagacc
      481 ctgtattgcc gaagcggaac ccggatttaa gacggtgcag acagaaacct tcgcgccgat
      541 tttatacctg ctgaaataca aaaccctgga agaggcgatt gccatacaaa atgatgtgcc
      601 gcagggtctt tcttcctcga tcatgaccct taacctccgg gaagccgaac agtttttgtc
      661 tgctgccggc agcgactgcg gaatcgccaa tgtgaatatc ggcacttccg gtgcggagat
      721 cgggggcgcc ttcgggggtg aaaaagaaac cgggggcggc cgcgaaagcg gaagcgatgc
      781 ctggaaatcg tatatgcgcc ggcagaccaa taccattaat tattcaactc aattgccttt
      841 ggcacaggga attaaattcg atttataagg gttcccggta aagcggacgg caattcccgg
      901 acggaccggg cttttatggc cggtttccgg gtataatata atgattatcc atccgttaat
      961 acaggatact cagatgaaat gaactttacc cggataagca cccgtttttc aattcttttt
     1021 ctcttcctgc atttttccca ttccacggca gcccagatcc ggctggcggc ggtcgccggc
     1081 gttcattccg ccactttcca ggaaaccaat tctcttccgg gttttgatag tacgcaaggc
     1141 cggttcactt cttccaaaac aggggtacaa ctgggggttt tggcagagat accgcttgga
     1201 aataagaatt tctttctcca gcccggcatg aactacagct cgaagggaag ccagtacgaa
     1261 agattttatg acaccctttc cggtcagaag gatacgctgt acaatcagta taccctgaaa
     1321 ctgaattata ttgaagtacc gctttacctg acttacaaat tcccgctttc taaaaacaag
     1381 cccaattatt tctatctggg agccggtcct tattttgcct ttatttacaa cgcttcgaag
     1441 ccttatcaaa accgggtgaa atattacggc aataatgatt acacctacaa ttcaggcact
     1501 caggatctgg aagtgggtaa tggtccgggc aaatacaaaa cgctggactt cggcatcaac
     1561 gcaaaggccg ggttcgaact gggaaacgta ctgctcggcg cttatttcag ccggggcctg
     1621 accaatgcct acgaagcgtc ttacccctcc agctttcacc atcagctgat cggagcctcc
     1681 ctgggaatat ggctgaacaa gatcaaaccg gtccccccgc ccaatacgga cagcgatcac
     1741 gacggcaccc ccgaccggga cgactcctgc aaaaccattc cggggtctcc caggtatcat
     1801 gggtgcccgg ttccggatac cgaccgggac ggggtgaacg atgaagcgga ttcctgcaag
     1861 aatgtgccgg gattggcccg ctatcacggt tgcccaatcc cggatacaga cggggatggc
     1921 gtgaatgatg aagaagattc ctgtaaaaca attcccggaa cggcaaaata tcatggatgc
     1981 ccgattccgg ataccgatgg agacggggtg aatgatgaag cggatcattg tcccgaccag
     2041 gccggcctgc ccgaaaataa tggctgcccg gtagtcccgg ttaaactggc caaacgcatt
     2101 ttgtttgttg cgaagaatgt tcaattccgc agcaacagca gcaaactgac cccgaattcc
     2161 ctgcccgccc tccgggagct cgcagatacc ctgaaagcaa atcccgacct ggaactggtt
     2221 gtggaggggc atacggataa ttccggatcg ccggagtaca atcaaactct ttctgaacag
     2281 cgggcggcgg aagtgaaaaa agccctgata aaactgggcg tgccggaaga actgatttca
     2341 acccggggtt atggcgacac gcagcccagc ggggataaca gaacaaggga agggaaatcg
     2401 aagaaccgcc gggtcgtatt ccggctgcat gttaaaaaca gttaaattga cggatgattt
     2461 tgttaccatt catgtaaaaa gtgagctgcg gtttgtgtgc tgatatcctt taaaatacat
     2521 ttaccagtca tatttaaacc taaacgaaat gatccgtttg aagcaaacga tgctggccct
     2581 attcctctta acggtcagcc tgcgcccttt tgcccaggga acttcttcca gaaccgaact
     2641 cccgaagggc tggcatctcc tcgatgaaga aaaagacggc gtctacggga tcagccttca
     2701 aaaagcatac gactttctcc atgccaggaa tttaaaaagc aaaaccgtcc tggtcgcagt
     2761 gattgattcg ggagtggata cattacagga agacctttcc catatcctct gggtcaatcc
     2821 cggagaaata cccggcaatg gcattgatga cgaccacaac ggttatatag atgatgtgca
     2881 cggatggaat ttcatcgggg gtaaggacgg aagcaatgtg aaggatgatt cgcaggaaga
     2941 aggacgcgtg tattaccggt acaaggacca gtttgacaac aaggatatcc gccccgacag
     3001 cctgaatgcg acagaccggg atcattatga tatgtggctg attgcgaaaa agaagatcat
     3061 gggagacgga acagagagcg gcattgacct gctgatgctg aaaagggtgg tgaatgcgtg
     3121 catcaagagc gatagtattt tgcaaaaagc catgggtaaa acagaatata ccggaaatga
     3181 tgtagatagt ttccaggcaa ccgatgttga cgccagaacg gctaaatccg ttttgctttt
     3241 cctgtttaag gagaaccata tcatggatat gaccaatacc acctttctca cggatttcaa
     3301 ggagcaggca gaccgggagg aaaagaagat ggaaataaag gaaacacctc ccagaaatta
     3361 ccgggctgaa attgtaaaag acaatgagaa cgatataaat gacagcattt acgggaacaa
     3421 cgatatcatg gcgggcgacc ccatgcatgg cacgcatgtt tcgggcatca ttgccgcgga
     3481 aaggaacaat aacaagggaa tgaacgggat cgcggacaat gtccgcatca tgatgatccg
     3541 ggcggtgccc aacggagatg agcacgacaa ggatattgca ctggccatcc gctatgccgt
     3601 ggataatggc gcgaaagtgg tgaatatgag ttttggtaaa aatttgtccc cggaaaaatc
     3661 ctgggtggat caggcggtgg aatatgcgga atccaagggg gtgttgctga tccatgccgc
     3721 aggaaatgat gcagaagatg tggatagtgt gggtaatttt ccaaaccctg tcattaagga
     3781 ttccaacaag agagcagaca actggatcac cgtaggcgcc agcagc
//
LOCUS       DASYCU010000225         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3675537, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000225 DASYCU010000000
VERSION     DASYCU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3675537"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VGM24_04605"
     CDS             complement(<1..1257)
                     /locus_tag="VGM24_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4154678.1"
                     /translation="MKVAIQPKRVVLWLTVLCCFSFIQARAQSISGTVVSSENSKPLE
                     GVSVSFGQKRGGTTTDAEGHYRLQAAKGDGKLVFSYTGYGTQEVSIGDRTVINISLVI
                     NSRDLSEVVVTAFGIEKQKRSIGYSTQVVKGDELTVARETNVASSLKGKVAGVFVSPS
                     NTGDGGSTFVNIRGASSFLGNNQPLYVVDGVPIDNQTIDMPDLNNDIGSSRDYGDGIG
                     NILPDDIETITVLKGPNAAALYGSRGAKGVILITTKKGKPGKNAVIDFNSNSVFERPN
                     ILPVYQNVWGPGYSQNTDGWNTDNVNGQQVLELPNWTGGDTWGPKYDGRPIVLQEWPD
                     AGVLKFSPQPSDELSKFYRTGSTFTNTIGVSGGNEKGNYRLSISDLHNQGIYPTSQLD
                     RQTVNLRVGFNATPKLYIEGKINYIRQ"
     gene            complement(1391..2248)
                     /locus_tag="VGM24_04610"
     CDS             complement(1391..2248)
                     /locus_tag="VGM24_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4154679.1"
                     /translation="MKRPWLLFALITTLFWGVWGAFIEIPEKAGFPATLGYCVWAITM
                     VPCALIALKIIHWKLETDMRSVIYGSIIGLTGAGGQLILFEALRDGPAYIVFPVVSLY
                     PVVTIVLSIVFLKESASRRNWTGIFLALVAIIFLSYVKPGGNTVTGFAWLWLAILVFL
                     LWGLQAFVMKFSNTTMKAESIFFYMMLSALVLIPIAIGMTHPSRPVNWGFHGPYLAAL
                     IQVLNSIGALTLVYALRYGKAIVVVPLTALAPVITIVLSLAIYQVFPHPIVILGLVLA
                     VAAIYFLSE"
     gene            complement(2275..3054)
                     /gene="agaR"
                     /locus_tag="VGM24_04615"
     CDS             complement(2275..3054)
                     /gene="agaR"
                     /locus_tag="VGM24_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor AgaR"
                     /protein_id="HEY4154680.1"
                     /translation="MGKPDRTTTVGRRVLILEKLDQEKQVDVISLSRELNVSEVTIRN
                     DLSRLEKKNMLIRARGGAIKVDHVGIDFNLSDKNKQHFKEKQRIGRAAADQIEDGDTI
                     ILDSGTTTMEIARNLENKTDLTVITNALNIANQLAELKNGNIIIPGGFLRKNSLSLIG
                     STAEESFRNYFCDKLFLAVDGINSTHGLSTPNVEEAHLNRVMIEISKQVIVVADSSKF
                     FKRSLAFIAPVTEVDVLVTDSGIAPDEKKKLENLGVKVIIV"
     gene            3247..>4299
                     /locus_tag="VGM24_04620"
     CDS             3247..>4299
                     /locus_tag="VGM24_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HEY4154681.1"
                     /translation="MDRFVENSDKILGYFLSNLAKQGGLQTAREIIQQPALWLSTWRL
                     LLEKKDELGSFLQEAGRDGSPDIILTGAGTSAFIGNILQGPFMKHTGNQTRAVASTDL
                     ITHPRNYFKKQGTTLLISFARSGDSPESVAASELAEICCDKVYHLIITCNPSGKLAKG
                     NRKFPTCVFLLPPESDDQSLVMTGSFSAMLLAGLLISRIGELEQLEDEMQRLSAYGSR
                     VIEQYSDKLKEIAGMDFKRAVFLGSGPLQGAARESHLKIQELSGGKIICKCDSFLGFR
                     HGPKAVINSSTLLIYLFSSDPFVHQYETDFVKSVNHGEKGLCQIGIFESGENNLGLDE
                     VIAFSEDREKLDEDFLS"
BASE COUNT         1041 a         1062 c         1139 g         1057 t
ORIGIN      
        1 ctgccggata taatttattt tgccttctat atacaatttc ggtgtggcat taaaacctac
       61 ccgcaggttg accgtttgcc ggtccagctg cgaagtcgga tagatcccct ggttgtgcag
      121 atcggatatg gataaccggt agttcccttt ttcattgccg ccggatacgc cgatggtatt
      181 ggtgaaagtg gatcccgtcc ggtaaaattt gctgagttcg tcggagggtt ggggggagaa
      241 ttttaatacg cccgcatccg gccattcctg caacacgatt ggcctgccgt catatttggg
      301 accccaggtg tctccgccgg tccagttcgg caattcaagc acctgctggc cgttcacgtt
      361 gtcggtattc cagccgtcgg tgttctgtga gtatcccggt ccccacacgt tctggtaaac
      421 cggcaggata ttcggtcttt cgaaaacgga attactgttg aaatcgatga cggcgttctt
      481 tcccggtttt ccttttttcg tggtgatcag gataacgccc ttggcgcctc tggatccgta
      541 cagcgctgcg gcattcgggc ctttcagcac ggtgatggtt tcaatatcgt cgggaagaat
      601 atttccgatg ccgtcgccgt agtcccggct cgatccgata tcgttgttta aatccggcat
      661 gtcgatggtc tggttatcga taggaacgcc atccaccaca tagaggggct ggttgttccc
      721 gagaaaggaa ctggcgccgc ggatattgac aaaagtggat cctccgtcgc cggtattgga
      781 aggactaaca aaaacaccgg ccacttttcc tttcagggaa ctggccacat tggtttcgcg
      841 ggcaacggtc agttcatccc ccttcactac ctgggtggag tagccgatgg atcttttttg
      901 tttttctatg ccgaaggccg taaccactac ttcggagagg tccctgctgt tgatgaccag
      961 ggaaatatta atcaccgtcc ggtcgccgat agagacttcc tgcgttccgt aaccggtata
     1021 ggaaaatacc agcttcccgt ctccttttgc cgcctgcagg cgataatgcc cttcggcatc
     1081 ggtcgtggtg cctccccgtt tttgtccgaa ggagacgctt accccctcga ggggtttact
     1141 gttttcggag gagaccactg ttcctgaaat ggattgggcc cgggcctgga taaaagagaa
     1201 acagcagagc acggtcagcc ataagacaac tcgttttggt tggatagcta ctttcatttc
     1261 gttatttttt tagatgaagg cgcctcataa atgtaaataa aaagcataat aacaaaaaaa
     1321 taattttcgt ttccttatta aaatagaata aaagcccgat ccgggaagat ctttctggtc
     1381 ggtgaagcgg ttattcggag agaaaataaa tggcggcgac cgccaggacg agaccgagga
     1441 taacgatggg atgaggaaag acctgataaa tggccaggga gaggacgatg gtaatcaccg
     1501 gcgccagcgc cgttagcgga accaccacaa tggccttacc gtagcgcagg gcatagacca
     1561 gggtcagggc cccgatggaa ttcagcacct ggatcagggc agccaggtaa ggaccatgaa
     1621 atccccagtt gaccggacgg gaaggatggg tcatgccgat ggctatggga attagaacca
     1681 gcgcggacag catcatataa aaaaaaatgc tttccgcctt catggtcgtg ttggagaatt
     1741 tcatcacaaa agcctgtagg ccccacaaca ggaaaaccag gatggccagc catagccagg
     1801 caaaaccggt aaccgtattg ccgccgggtt ttacatagga caggaaaata atggccacca
     1861 gggccaggaa gattcccgtc cagttccgcc tggacgcgga ttcctttaag aaaacgatgg
     1921 ataaaacgat cgtcacgacc gggtaaagcg aaactaccgg aaaaacaata taggcgggac
     1981 cgtcgcgcaa ggcctcgaag aggatcagct ggccgcctgc ccccgtgaga ccgatgatgc
     2041 tgccgtagat caccgagcgc atgtcggtct ccagcttcca gtgaatgatt ttaagagcga
     2101 tcagggcaca gggcaccatg gtgatggccc agacgcaata acccagggtg gcggggaatc
     2161 cggccttttc cggaatctcg ataaaagcgc cccatacgcc ccagaaaagg gtagtgatga
     2221 gggcaaataa caaccagggt cttttcaagg ggaatgaatt gcggagataa atagttaaac
     2281 gatgatcact ttaacgccga gattttcgag cttctttttt tcgtccgggg cgatgccgga
     2341 atccgtcacc agcacatcca cttcggtcac cggcgcgata aatgccaggc tccgcttgaa
     2401 gaatttgctg gaatcggcca ctacgatcac ctgtttggaa atttcaatca tgacccggtt
     2461 caggtgggct tcttccacgt tgggggtgga aagaccgtgg gtggaattga tgccgtcgac
     2521 ggccaggaat agtttgtcgc agaaataatt ccggaagctt tcttccgcgg tggaaccgat
     2581 cagcgataag gaattcttgc gcaggaatcc gccagggata atgatattgc cgttcttgag
     2641 ttcggccagc tggtttgcaa tattcagggc gttggtgatc accgtgaggt ccgtcttgtt
     2701 ttcgagattg cgcgcgatct ccatcgtggt ggttccggaa tcgaggatga tcgtatcgcc
     2761 atcctctatc tggtctgccg cggcccgccc gatgcgctgc ttctctttaa agtgttgttt
     2821 attcttgtcc gaaagattga aatcgatgcc cacatggtcc actttgatgg cccctccgcg
     2881 ggcgcggata agcatattct ttttttccag cctggacaga tcattgcgga tcgttacctc
     2941 gctgacattt aattccctgc tcagcgaaat cacgtccacc tgcttttcct gatccagctt
     3001 ttccaatatt aatactcttc gcccgacggt tgtggttcta tccggtttgc ccataaagaa
     3061 agattggttc tcttaatatg cgtactaagt tatccatttg ccaagaccct gattcatttc
     3121 cgggacagat gaattttttt ttaactcccg ggtgtgctaa aagtaaggga ataaaaaata
     3181 tttacaaaag gaattataaa ttattatatt tatatcgaaa tgaaatgaaa aacaaaaaca
     3241 gccaaaatgg atcgatttgt ggaaaactcc gataagatat taggttattt cctttcgaat
     3301 ctggcgaagc agggtggttt acaaaccgcc cgggagatta tccagcaacc ggcactctgg
     3361 ctcagcacct ggcggctgct gctggaaaaa aaagacgagc tcggaagttt tctgcaggaa
     3421 gcgggccggg atggttctcc ggatatcata ctcaccgggg ccggcacctc cgctttcatc
     3481 ggtaatatcc tgcagggtcc gtttatgaaa cataccggga atcagacccg ggcggtcgcc
     3541 agcacggacc tgatcactca tccccggaat tattttaaaa agcaggggac gaccctgctg
     3601 atctcttttg cgcggtcggg cgacagcccc gagagcgttg ccgcttccga actggcggaa
     3661 atatgctgcg ataaggttta tcatttaatc attacctgca atccttcggg aaaactggct
     3721 aaggggaacc ggaaattccc cacctgcgtt ttccttcttc ctccggaatc cgacgatcag
     3781 agcctggtga tgaccggcag cttttccgcc atgttgctgg ccggactgtt aatctcccgc
     3841 atcggggagc tggagcagct ggaggatgaa atgcagcgcc tgtcggctta cggaagccgc
     3901 gtgattgaac agtattccga taaacttaaa gaaatagccg ggatggattt caaacgcgcc
     3961 gtgtttctcg ggtcgggccc gctgcagggc gctgcgcggg aatcccatct gaaaatacag
     4021 gaattgtcgg gaggaaaaat aatctgtaag tgcgattcct ttctcggctt caggcacggg
     4081 ccgaaagcgg tgatcaattc ctccacgctg ctgatctatc ttttctccag cgatcccttc
     4141 gtccaccagt atgaaacgga tttcgtcaaa tcggtcaacc atggcgagaa aggcctctgc
     4201 caaatcggta tttttgaatc cggggagaat aatctgggac tggacgaggt gatcgcattt
     4261 tccgaagacc gggagaaact ggacgaagat tttttgtcc
//
LOCUS       DASYCU010000226         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1500419, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000226 DASYCU010000000
VERSION     DASYCU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500419"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGM24_04625"
     CDS             <1..333
                     /locus_tag="VGM24_04625"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY4154682.1"
                     /translation="CASSLQFTTWKTRQLACCRQLPGCNRPMPADARTAWLQGLRFGF
                     HCVRCCAGLMAILLAIGVMNLYAMAIVAVAISIERLSPAPAWPVRTIGLAGIGTALWM
                     IWQAAGPV"
     gene            complement(362..877)
                     /locus_tag="VGM24_04630"
     CDS             complement(362..877)
                     /locus_tag="VGM24_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154683.1"
                     /translation="MKIIWTFFFFLGSLISSKAQEMLPDTNALKSVDGIVKEMLRLES
                     GKKGQIRNFKALRNLFLPTATFTILNDSDFAGQPTETVSLDDFMKLLHDEYYEEGYLE
                     YETGKTIDEFNGIANVFQSFYGKDSEKREARGINSYQLVYFGKRWWIASILWTLESNK
                     VKIPKKYLRQN"
     gene            complement(1318..1695)
                     /locus_tag="VGM24_04635"
     CDS             complement(1318..1695)
                     /locus_tag="VGM24_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4154684.1"
                     /translation="MKKVTGIGGIFFKCKDPKAINEWYKTHLGFDTSPYGTSFEWREI
                     DDSTKKGLTQWNPFAENTKYFEPSTKDFMINYRVENLEALVEDLKKEKVTIVDKIESY
                     DYGKFVHILDPEGNKIQLWEPKD"
     gene            1974..2681
                     /locus_tag="VGM24_04640"
     CDS             1974..2681
                     /locus_tag="VGM24_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4154685.1"
                     /translation="MPVEQFNMEKGYAVFSRGNYQTGRHFHYAIEIVFAGKGSFGIST
                     GLSEYANLQSVIIPSNLPHSFSCLNADCNLLFLDPLSDMGLFFLQEYDLNAQKTIIAD
                     PPGLDPFHKKGKFDIPLILESAKTIRTRPVDSRISACMQAISSSLTDEELTVSQLSKV
                     SFLSESRLAHLFREQLGISVHQCILWKKIMLAVLKSGEGYSLTECAHYAGFSDSSHFN
                     KVFYRMFGVNPFFVLKT"
     gene            2750..3133
                     /locus_tag="VGM24_04645"
     CDS             2750..3133
                     /locus_tag="VGM24_04645"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4154686.1"
                     /translation="MMETKERTMKIGEIARHFVHWRNTDEAARLRTELYSPEIESIED
                     GMVNEAGRVKGMEGLRKKGARLSQDFEVHGIKASDPVVADNWFSVKFEVDATDKNSGE
                     RSRLSEIGVYKVENGRIVKEHYFMW"
     gene            complement(3173..3949)
                     /locus_tag="VGM24_04650"
     CDS             complement(3173..3949)
                     /locus_tag="VGM24_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4154687.1"
                     /translation="MARIFITGSSDGLGRMAARLLVSEGHEVVLHARNRERAAAARTG
                     VPGAEEVLTGELSDIKETIQLAEQGNRSGPFDAIIHNAGIGSGERRRIETGDGLSHVF
                     AINSLAPYILTCLITRPGRLIYLSSGLQQGGTPDMTDLAWQHKPWNGFQAYSDSKLQD
                     LMLAFAVARKWPSVYSNGVDPGWVATKMGGPGAPDNLEEGAKTQVWLAVSDDPAAQST
                     GKFFHHRKIRKPNPAAESPQIQEEYLKHCARFSGIAFPEK"
     gene            4127..>4918
                     /locus_tag="VGM24_04655"
     CDS             4127..>4918
                     /locus_tag="VGM24_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154688.1"
                     /translation="MKTAKVFLTGSSFFLLVYFISCRKNVDSPVPTDIPQLPDSVAPL
                     QTIPNQDSNGYHPFPENYSMNCPGSPSYGDSIVYPSGNYGAPDYLLSPVNNPEPGHYY
                     SWPVGLVINDSTGTINVTRSETGLRYFIGYVKKGSQDTCINTLIIGGAAYIDSIYTQS
                     RPGPNRVLPYFNANSGINSLCGPGPLGHCWWDLSQNAKNQKIVLDEHTGVIDLDQTIQ
                     KGAFGKNPVDGATLNAVIYYMIGEGNSGIQSLKIQLMYYDRASSIP"
BASE COUNT         1200 a         1240 c         1121 g         1357 t
ORIGIN      
        1 tgcgccagtt ccctccagtt taccacgtgg aagacccgtc aactcgcctg ctgcaggcag
       61 ttgcccggat gcaaccgccc gatgccggcc gatgcccgga ccgcctggct ccagggcctg
      121 cggttcggat tccactgcgt ccgctgctgt gccggactga tggcaatcct cctggccata
      181 ggagtcatga acctttatgc catggccatc gtagccgtgg cgatcagcat cgagcgtctc
      241 tctcccgctc ctgcgtggcc ggtgcgaact atcgggctcg ccggcatcgg taccgcttta
      301 tggatgattt ggcaggcagc cggtcctgta tgaaacagtt tgctgcaaca cgtagcggac
      361 tctaattctg gcgtagatat ttttttggga tttttacttt attggactcc aatgtccaaa
      421 gtatacttgc aatccaccac ctttttccaa aataaactaa ctgataacta ttaattcccc
      481 gggcttccct tttttcagaa tccttaccat aaaagctttg aaaaacattt gcgattccgt
      541 taaactcgtc aatggtttta ccggtttcat attcgagata accctcttca taatactcat
      601 cgtgcaagag tttcataaaa tcatccaggc taaccgtttc cgtcggctgg cccgcaaaat
      661 cgctgtcatt taaaatagta aatgttgctg ttggtaaaaa caggtttcgc aacgccttaa
      721 aatttcttat ctggcccttt ttgccggatt caagtcttaa catctctttt acgatgccgt
      781 caaccgattt gagagcattg gtatcgggca acatttcctg tgcttttgaa ctaatcaggg
      841 aacctaaaaa gaagaaaaat gtccagatta ttttcatttt ccaatggaat ttactgtccg
      901 tttccggaaa ccgtttaact tctgctgcat cagctaataa gcccggtttg aaggcaaatg
      961 ggagaatttt gcggtttttc ctggggccgg acagcgcgct cgcaggccgg cttttgcggc
     1021 ttctgatcgg actctggtca tactctggtt ttactctgat ccctctcaga tctctgaccc
     1081 tggtctcgtt ctggtcccgt tctgatcttg cttcgtaccc ctctttccca acccccggaa
     1141 ttttcaataa tagccatatt tatttcctgg cactccgcca aaactccgaa aaatcctctt
     1201 tttgtaaccc tattctaaga atgatgaaat gcttatggcc tgtttttaga ggaatttgcg
     1261 ccaatggcct gacagtcagc acaattggga tctctttgta tccaaaatgc ctgcggctca
     1321 atccttaggc tcccacaatt gaattttatt tccttccggg tccaggatat gaacaaattt
     1381 tccatagtca taggattcga tcttgtcaac gatcgttacc ttttcctttt tcaaatcttc
     1441 caccagcgcc tctaaattct ccacccggta gtttatcata aaatcctttg tggaaggctc
     1501 aaaatatttt gtgttctctg caaacggatt ccactgggtt agcccctttt ttgtactgtc
     1561 atcgatttcc cgccactcaa agcttgttcc gtatgggctg gtgtcaaacc ccagatgcgt
     1621 cttataccat tcgtttatgg ctttaggatc tttacattta aagaaaattc cgcctattcc
     1681 cgttactttt ttcattttgg tgtttttaat tttgttagaa attatggtcc tgaacgtgaa
     1741 ccctgaaaga aaggaagccg caatgagcgt gatggtccat gctgtccgtt tcattgtctt
     1801 taaaattaaa atttataatt gagcattact tatttaccat gaacagcgga gtcctgtcca
     1861 aacaggctcc ggaccggata aatccacttt tggaatccga ctcaaattga aataataatt
     1921 atggatttaa caagaataaa ttgcctgaaa aaccaggctt tccgttaaaa cacatgccgg
     1981 tagaacaatt taatatggaa aaggggtatg ccgtattttc caggggaaat taccaaaccg
     2041 gcaggcattt ccattacgcc atcgagatcg tatttgccgg taagggctcg ttcgggatca
     2101 gcaccgggct ttccgaatat gcaaaccttc agagtgtaat tattccgtca aaccttcccc
     2161 atagttttag ctgcctgaat gccgactgca accttttatt tctggacccg ctctcagata
     2221 tgggattatt ttttttacag gagtatgatc tgaatgcgca gaaaacgatt atcgccgacc
     2281 ctccgggatt agatccgttt cacaaaaaag gaaaattcga catcccattg atcctggaaa
     2341 gcgccaaaac aattcgtacc cggcccgtgg acagcagaat ttccgcctgt atgcaggcca
     2401 tcagctcttc cctcacggac gaggaactga ccgtatccca attatcgaag gtttcttttt
     2461 tatcggaaag ccggctggcc catttgttcc gggagcaact ggggatttcc gttcaccaat
     2521 gcatcctttg gaaaaaaata atgctggcgg tattaaagtc aggagaagga tattccctga
     2581 ccgaatgcgc gcattatgcc ggtttctcgg attcttccca tttcaataaa gtattctacc
     2641 ggatgtttgg cgtcaatcct ttctttgtcc ttaaaactta gcaggtttat acaatttttt
     2701 tattccccgt ctttggagct ttgtgtcctt attcattata aaaataaaaa tgatggaaac
     2761 aaaggaaaga acaatgaaga tcggcgagat cgcccggcac ttcgtgcatt ggaggaatac
     2821 agacgaggcg gcccgcctgc gtacggaact gtacagcccg gaaattgaaa gcatagagga
     2881 cggaatggtc aatgaggctg gccgggtgaa aggcatggag ggcttaagga aaaaaggagc
     2941 ccgtttgtct caggattttg aagtacatgg tatcaaagca tctgatccgg tggtggccga
     3001 taactggttc tccgtaaaat tcgaagtaga tgctacggat aaaaactcgg gagaacgttc
     3061 ccggttatcc gaaataggcg tttataaagt ggagaacggc cggattgtaa aagaacacta
     3121 ttttatgtgg tagtgaatcg cggtcgcgct ggtactattt ataatcctga tcctattttt
     3181 ccggaaatgc aattccggaa aaccgggcgc agtgctttag gtattcttcc tgaatctgcg
     3241 gactttcagc agccggattg ggtttccgga ttttgcggtg atgaaagaat tttcccgtcg
     3301 actgcgccgc gggatcatca cttaccgcaa gccagacctg cgttttcgct ccttcttcca
     3361 ggttgtccgg cgctccgggg ccgcccattt tggtcgccac ccagccgggg tcaaccccat
     3421 ttgaataaac agaaggccat ttccgggcaa ccgcaaaggc cagcatcagg tcctgcaact
     3481 tggagtccga ataggcctgg aaaccgttcc agggcttgtg ctgccaggcc agatccgtca
     3541 tatccggagt tccgccctgc tgcaggccgg aactcaggta gatcaggcgc ccgggacggg
     3601 tgatcaggca ggtcaggata tagggggcca ggctgtttat agcgaaaaca tgagataagc
     3661 cgtccccggt ttcgatgcgg cggcgttcgc ccgacccgat acccgcattg tgaatgatgg
     3721 catcaaaggg gccgctccgg ttcccctgct cagccagttg gatcgtttct ttaatatcgg
     3781 acaactcgcc cgtaagcact tcctccgctc ccggaacccc ggtgcgggca gccgcggccc
     3841 gttccctgtt tctggcatga agcaccacct catgcccttc actcacgagc agccgggccg
     3901 ccatgcggcc aagtccgtcg gatgaaccgg tgatgaatat gcgcgccata ggtcttttcc
     3961 taaatgtatg caatcttgca accataaaaa gccgcaccat ccggcaggcc ggaaggctct
     4021 gaaaggcagt ggggcgcatt aattcagtat tgggtaaaat tttccatact gggaaatttg
     4081 cacttactta ttttgcgccg tattttgggt tatacccttt aaccttatga aaacagccaa
     4141 ggttttcctt accggttcat cttttttcct tcttgtttat tttatttcct gccgaaagaa
     4201 tgttgacagc ccggtaccaa ccgatattcc gcagttaccg gattccgtag caccgctgca
     4261 gaccattccg aaccaggaca gcaatggcta ccaccccttt cccgaaaatt attcaatgaa
     4321 ttgccctggc agtccctctt atggggatag catcgtctat ccctccggaa attatggagc
     4381 tccggattac cttctgtctc ccgtaaataa tccggaacca ggtcattatt attcctggcc
     4441 cgtcggcctg gtgatcaatg attccaccgg aacgataaat gtgacccgga gcgaaaccgg
     4501 tctccgttat tttatcggtt acgtaaaaaa aggatcgcag gatacctgca taaatacact
     4561 gatcattggc ggggctgcat atatagacag catttataca caaagcaggc ccgggccgaa
     4621 ccgggtcctg ccctatttca atgcaaatag cggcatcaat agcctgtgcg gcccgggccc
     4681 gctcgggcat tgctggtggg atcttagcca gaatgcgaaa aatcaaaaga ttgtcctgga
     4741 cgagcacacg ggggttatcg atctggatca aaccattcag aagggcgcat ttggaaagaa
     4801 tccggttgac ggggccaccc ttaatgcggt catttattac atgattggcg agggtaacag
     4861 cggcattcaa tccctaaaga tccagttaat gtattatgac cgggcctctt ccattccg
//
LOCUS       DASYCU010000227         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_775428, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000227 DASYCU010000000
VERSION     DASYCU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775428"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VGM24_04660"
     CDS             <1..202
                     /locus_tag="VGM24_04660"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628351.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY4154689.1"
                     /translation="YDSLIAKIIAVARTRNEAIDTMHRALTEYVIEGIKTTIPFHLQL
                     MQDEQFRSGNFNTKFLDGFKMQ"
     gene            complement(217..1443)
                     /locus_tag="VGM24_04665"
     CDS             complement(217..1443)
                     /locus_tag="VGM24_04665"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY4154690.1"
                     /translation="MKAFQIPLTYRSPLITAVKQKRRAADKMKKDFSPVLLDFGPLQV
                     YLARHFGFCYGVENAIDIAFRTVEENPGRNIFLLSEMIHNPQVNRDLEERGVRFLQST
                     AGKQIIPFDDLKEEDIVITPAFGTSLETDAILKSKNIHTEQYNTTCPFVEKVWNRSGQ
                     MAERNFTVIIHGKPGHEETRATFSRAAAGGPALVIRDMEEARLLAPFIRREQEPEAFF
                     ETFKTRISSGFDPAKDLDRIGVVNQTTMLASETQGIADFLKQVVLDHRGSTEQKTGGL
                     FADTRDTLCYATNDNQSAVIALLETPADLAIVVGGYNSSNTSHLVELCEQKLPSYFIS
                     SEEKLLSKTEILHFDLARQQEILDSGYLKGDDPVKILITSGASCPDAVVESVITRLAG
                     FFEGSIPAETLMKEFL"
     gene            1536..2810
                     /locus_tag="VGM24_04670"
     CDS             1536..2810
                     /locus_tag="VGM24_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY4154691.1"
                     /translation="MNPVFHPLPWSSDSNIYEVNLRQYTPEGSFSAFEAHLPRLRDMG
                     VEMLWFMPVTPISRKGRLGSLGSYYACSDYITTNPEFGSLEDFKSLVQAAHKLGFKVL
                     IDWVANHTGWDHRWTTEHPEFYRRNSAGNFFDPHGWEDVIDLNYELPQLRKAMIQSMK
                     FWVDQCDIDGFRCDMAMLVPLDFWMEARTTLDRVKPLFWLAECEEISYHAVFDASYTW
                     KLMHSMEDFWKKPGSLDGLYEVLRFYNDQYPPHAQRALFTSNHDENSHSGSEYERMGE
                     AALPFAVFCCTWNGIPLIYSGQELPNRKRLKFFDKDLIDWKMPCALHDFYKALLQVRK
                     TSPALRSGDPSVSTILLPDDANRQLLSFLRRKDANEVLVILNLSAGEVSMSLSGAVAS
                     GSFVDIFSRENLRIGPGTNITLKPWDFRVYVK"
BASE COUNT          722 a          782 c          784 g          780 t
ORIGIN      
        1 ttatgattcc ctgatcgcca agatcattgc agtggcgcgt acgcgtaacg aagccattga
       61 caccatgcac cgggcactca cggaatatgt gattgaggga atcaagacca cgattccctt
      121 tcacctgcaa ctgatgcagg atgaacagtt ccgcagcggc aatttcaata cgaaatttct
      181 ggatgggttt aaaatgcagt gagcgccagc gagggttcaa agaaattcct tcatcagggt
      241 ctccgcggga atgctccctt caaagaaccc cgccaggcgg gtaatgacgg attccacgac
      301 ggcatcgggg caggaggccc cgctggtgat cagaattttt accggatcat ctcctttcag
      361 gtatccgctg tccaatattt cctgctgccg ggccaggtcg aaatgaagaa tttccgtttt
      421 ggaaagtagt ttttcttccg agctgatgaa atagctggga agtttctgtt cgcacaattc
      481 caccagatgt gaggtgttgg aagaattgta gccgccgaca acgatcgcca gatcagcggg
      541 cgtttccaac agggcgatga ccgccgactg attgtcgttc gtggcgtagc aaagcgtatc
      601 acgcgtgtcg gcaaaaagac cgccggtttt ctgttcggtt gaaccgcggt gatccaggac
      661 aacctgtttg agaaaatctg caatgccctg ggtttccgaa gccagcatcg tggtttgatt
      721 caccaccccg attcggtcca ggtcctttgc cgggtcaaat ccgctgctga tgcgggtttt
      781 gaacgtttca aagaaagcct ccggctcctg ttcgcgccgg ataaatggag cgagcaagcg
      841 ggcttcttcc atatcccgga tcacgagggc gggaccaccg gcagcggccc gggagaacgt
      901 tgcccgcgtt tcttcatgtc ccggcttgcc atgaatgatc accgtaaaat ttcgctcagc
      961 catttgcccg gaccggttcc aaaccttttc cacaaaggga caggtggtat tgtactgttc
     1021 ggtatgaata ttttttgact tcagaatggc gtccgtttcc aggctggtcc cgaaagccgg
     1081 ggtgatcaca atatcttctt cctttaaatc atcgaaggga atgatctgtt tgccggccgt
     1141 actttggagg aagcgaactc cgcgttcctc caggtcccgg tttacctgcg ggttgtggat
     1201 catttcactc aacagaaaaa tatttcttcc cggattttct tccactgtcc ggaacgcgat
     1261 atcgatggca ttttccactc cataacaaaa cccgaaatga cgggcaagat aaacctgcag
     1321 cggcccgaag tcgagaagga ccggcgaaaa gtcttttttc attttatccg ctgcccggcg
     1381 tttctgttta acagcggtga tcagcggact tcggtatgtg agcggaatct gaaaagcctt
     1441 cataacgaaa gcaaagttat gccggccggc gcgggaaaaa aatacttaaa gaggcctttc
     1501 gtatcttccc ttttaatttc ttgtttacca tcctcatgaa ccctgtattt catcccttac
     1561 cctggagctc tgattcgaat atttatgaag tgaacctgcg gcagtatacg cccgaaggaa
     1621 gttttagtgc attcgaagcc catctgcccc ggctacggga tatgggagtg gaaatgctct
     1681 ggttcatgcc cgtaaccccc atcagccgga aagggcgatt gggttccctg ggaagttatt
     1741 acgcctgctc agactatata accaccaatc cggaattcgg gtcgctggaa gattttaaat
     1801 ccctggttca ggcggcgcat aaactcggtt ttaaggtgct gattgactgg gtggccaatc
     1861 acacgggatg ggatcaccgg tggacaaccg aacatcctga attttaccgc agaaattctg
     1921 cggggaattt ttttgatccc catggctggg aagacgtgat cgacctgaat tatgaactgc
     1981 cccagcttcg caaagccatg atccagtcca tgaagttctg ggtggatcaa tgtgatattg
     2041 atggctttcg ctgcgatatg gccatgctgg ttcccctgga tttctggatg gaagccagga
     2101 cgaccctcga ccgggtcaag cccctgttct ggcttgcaga atgcgaggaa atttcctacc
     2161 acgccgtttt tgatgccagc tatacctgga aactgatgca ttccatggaa gatttctgga
     2221 agaagccagg gagtctggat ggtttgtacg aagtgctgcg tttctataat gatcagtatc
     2281 ccccgcacgc ccagcgagca ctcttcacca gcaaccacga tgaaaattct cacagcggat
     2341 ccgaatatga aaggatggga gaagcggccc tgccgtttgc cgtattctgc tgcacctgga
     2401 atggaattcc cctgatttac agcgggcagg aattacccaa ccggaaaagg ttgaaattct
     2461 tcgacaagga tttgattgac tggaaaatgc cctgcgccct gcatgatttt tataaggccc
     2521 tgctgcaggt cagaaagacc agtccggccc tccgttcggg agatccttcc gtatccacca
     2581 tcctgcttcc ggatgacgcc aaccggcagc tgttgagttt tctccgccgg aaagacgcca
     2641 atgaagtatt ggtcatttta aatctttccg ccggggaggt ttcgatgagc ctttccggtg
     2701 cagtggcatc gggttcgttc gtcgacatct tcagccggga aaacctgcga attggtccgg
     2761 gaacaaatat tacattaaaa ccctgggact tccgggtgta tgtaaaataa gttgatcgct
     2821 gatggctgac ggaacctggt ttcggagtta taaccaggcc catcggccat cagcgatcaa
     2881 cgtttaaaat ttaattatca ttcaatttca acggataggt aaaggttccg tcgtacccgg
     2941 gataaatgcc ctccagtttc acggagccat tgccggccgc gtccagtatc ttgcgaaact
     3001 catccacggt agttacaggc tctccgttta cttttaagat cacgaatccc tcttccatcc
     3061 gggttttg
//
LOCUS       DASYCU010000228         5517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4401882, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000228 DASYCU010000000
VERSION     DASYCU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4401882"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGM24_04675"
     CDS             complement(<1..281)
                     /locus_tag="VGM24_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HEY4154692.1"
                     /translation="MSEEIINKVSQSALLTLDLEKYFPEEKIVLFDLKDFLFMELILK
                     EKDFRAALQGKDWSPYKDQVVGVYCSTDAIIPVWAYMLVASYLEPVARDV"
     gene            complement(432..2576)
                     /locus_tag="VGM24_04680"
     CDS             complement(432..2576)
                     /locus_tag="VGM24_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY4154693.1"
                     /translation="MSIIQQIRERAAWLVFGLIGVSLIGFLLMDSSVGNSRLNASNSG
                     NVGSVNGDQLDYVDFEKQISEREDQYKSQGYPLNDMVQQNIRESVWKQFEEEAVLKTE
                     YDKLGLDVSDKELNDMLVGNNAVPEIKRAFTDPNTGIFDPQQAAARINQLRTIYKANR
                     KTDQNYAVAQNFFEQVLPQFVKLRLKEKYISLLANSSYIPKWMIEKSNAENSQVASVS
                     YVNIPYRTISDSAIKVSDEEVQAYLDKHTDQYQQEDTRSIRYVTFSAAPTAGDSSSIR
                     QQVQNLKNEFSTTADIEGFLARNGTEINYHDDYTAKSKLTGSKKDSLSLLPKGSVIGP
                     YLDGPNYVIARIMDIKTLPDSVKARHILVATVDLRTNQPKLDDSTAKNRIDSIKNLID
                     KGARFDSVAFHLSDDEGTKLKGGDLGYFANGALVKEFNDFAFSGKVGEKKIIKTQFGY
                     HYVEIEDQKNFEPAYKIAYFARKIEPSSETDENASGLASQFAGENRTQAAFDENSKKE
                     NLQKGAAADIHPTDFNVQGVGSSRTLVRWIYDAKLGEVSESFSVGDKYVVAMVTEINK
                     KGTMPIAKARPLIEPILRNQKKAELIAKKIASAASLEAVASSTGQTITKADSLGFSSP
                     YIPNLGPEPRVVGYAFDKQLSGKGISAPVFGNEGVFVLKVNQVSARANYNGDIEQSRE
                     AMLQSQESIIQRAATEALKKKAKIKDNRAKFL"
     gene            3147..4148
                     /gene="nadA"
                     /locus_tag="VGM24_04685"
     CDS             3147..4148
                     /gene="nadA"
                     /locus_tag="VGM24_04685"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY4154694.1"
                     /translation="MNMDQAKMQITRKGFLDIPVNPELDLFEEIARLKKEKNAVLLAH
                     YYQDADIQDVADFIGDSLGLAQEAARTRADIIVFAGVHFMAETAKILNPDKKVLLPDL
                     NAGCSLADSAPAPLFRSFREKYPDHLVISYINCSAEIKALSDIICTSSNAEKIIDSLP
                     ADQKIIFAPDKNLGAWLIKKTGRNMVLWNGACMVHEIFSQKKITALKARHPEAKFIAH
                     PECEEPVLRMADFIGSTTALLKFTQKDPSKEFIVGTESGILHQMMLASPEKTFIPAPP
                     ETNCACNDCPHMKLNTLEKLYLCMEYEAPEIRMERSLLEAARKPIDRMLEISRQYGL"
     gene            complement(4149..>5517)
                     /gene="mnmG"
                     /locus_tag="VGM24_04690"
     CDS             complement(4149..>5517)
                     /gene="mnmG"
                     /locus_tag="VGM24_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEY4154695.1"
                     /translation="KQFGGGRMSEKASTGITEQLRSEGFESNRLKTGTPPRIDGRSLD
                     YSKMEEQPGDEKPVGFSYLNIPLPARQRSCWITYTNPAVHEILKTGFDRSPMFTGRID
                     GTGPRYCPSIEDKLNRFADKERHQLFIEPEGWNTIEIYVNGFSTSLPEEVQYEALRMI
                     KGFEHFKAFRPGYAIEYDYFPPTQLGHSLETKILNNLFLAGQINGTTGYEEAACQGLM
                     AGINAHRKINHLEPVILSRSEAYIGVLIDDLISKGTEEPYRMFTSRAEFRTLLRQDNA
                     DLRLTEKSHSLGLASEERLKAVNIKKNEVNEISFILNNFSVSPQEAEVFLGEMNLSPL
                     VQKQKASTLILRPLIGITQLKGQIPALAEKLESFPPESLEQAEIQMKYEVYIKKEKEL
                     VKRISQLEQLSIPETFDYDRISALSMEALIKFKKIKPRTLGQASRISGVNPTDIQILM
                     VYMGR"
BASE COUNT         1323 a         1310 c         1324 g         1560 t
ORIGIN      
        1 acatccctgg caaccggttc cagataactg gccaccagca tataggccca aacgggaatt
       61 attgcatctg tggaacaata gacgccgaca acctgatctt tatatggtga ccagtctttg
      121 ccctgaagtg cagctcgaaa atccttttct ttcaggatca gttccataaa aagaaagtcc
      181 ttcagatcga ataacacaat cttctcttcc gggaaatact tttccaggtc cagcgtaagc
      241 agggcgctct gactaacctt attgataatt tcctcagaca taaactgtaa atatcttctg
      301 caaaggtaag caggaaaatc ccgattctgc tgcaagcaaa aagcctttcc gtatgcgcgg
      361 taaggctttt gccggagagc taaatttttt ttctgaggcc tgcgttctga ttcaggccgg
      421 atgaccggga gttaaaggaa tttggcgcga ttatctttaa tctttgcttt tttcttaagg
      481 gcctctgtag cagcacgctg gataatggac tcctgcgatt gcagcatcgc ctcccggctt
      541 tgttcaatat ctccgttgta gttggccctg gcgctcacct ggttaacctt cagaacaaaa
      601 acgccttcat tgccaaagac cggggcagaa atgccttttc cggaaagttg tttgtcaaag
      661 gcatacccca ccactcttgg ctccgggccc aggttgggaa tatagggcga agagaacccg
      721 aggctgtcgg ctttcgtaat ggtctgcccg gtggaagaag caaccgcttc caggctggcg
      781 gcagaggcaa tttttttggc gatcagttct gcttttttct gatttctgag gatgggttca
      841 atgaggggtc ttgctttggc gatcggcatc gttccttttt tgttgatctc ggtaaccatg
      901 gccactacat atttatctcc gacggaaaaa gattctgaaa cctctccgag tttggcgtcg
      961 tagatccagc ggaccagcgt ccggcttgac cccacaccct gaacattgaa atcggtggga
     1021 tgaatatcgg cagccgcgcc tttctgaaga ttttcttttt tagaattctc atcgaaggct
     1081 gcctgagtcc tgttctcccc ggcaaactgg ctggccagtc cggaggcatt ctcgtctgtt
     1141 tccgaactcg gttctatctt ccgggcaaaa taagcaatct tgtaagcggg ttcgaaattt
     1201 ttctgatcct cgatttcgac ataatgatat ccgaactggg tctttattat tttcttttct
     1261 cccacttttc cgctgaatgc aaagtcattg aattccttta ccaaagcccc gttggcaaaa
     1321 tagcccaggt ccccgccttt aagcttggtt ccttcatcat cagataaatg aaaagcgaca
     1381 ctgtcaaacc gggctccttt gtcaatcagg tttttaatac tgtctattct gtttttcgcc
     1441 gtactgtcgt cgagtttggg ctggttggtc cggaggtcaa cggtcgcaac cagaatatga
     1501 cgggctttta cggaatccgg caaggtttta atgtccatga tgcgggcaat cacatagttc
     1561 ggcccgtcca gataaggtcc gatcaccgat cctttgggaa gcaggctgag ggagtccttt
     1621 ttggacccgg tcagtttgga tttggccgta taatcgtcgt ggtaattaat ttccgttcca
     1681 ttccgcgcca aaaatccttc tatgtccgcc gtcgtgctga attcattctt caggttttgt
     1741 acctgctggc ggatggaact gctgtcaccg gccgttggcg cggcgctgaa agtgacatag
     1801 cggatgctcc gggtgtcctc ctgctgatat tgatctgtgt gtttgtccag ataggcctga
     1861 acttcctcgt cagatacttt tatggcactg tcggaaatcg tgcgatacgg aatattcaca
     1921 tatgaaacgg aagccacctg gctattctcc gcattggatt tttcaatcat ccatttcggg
     1981 atgtaggaac tgtttgccag caaagaaata tatttctcct tcagccggag tttgacgaac
     2041 tggggcagca cctgttcgaa aaaattttgg gccaccgcat agttctgatc cgtctttctg
     2101 ttggctttat atatcgttct cagctggttg atccgggccg cagcctgttg cggatcaaaa
     2161 atcccggtat ttggatccgt aaatgctctt tttatttccg ggaccgcatt gtttccaacc
     2221 agcatatcat tcagctcttt atcgcttacg tccaggccga gcttgtcata ctctgttttc
     2281 aggacggcct cttcttcaaa ttgtttccag acggattccc ggatattctg ctgaaccata
     2341 tcgttcaggg ggtatccctg gctcttatat tgatcttccc gttcggaaat ctgcttctca
     2401 aaatcgacat aatccagctg atcgccgttt acagacccca cgtttcccga attagaggcg
     2461 ttcagccggc tgttacccac cgaagagtcc atcaaaagaa atccgatcaa gctcactccg
     2521 atcaggccga aaaccagcca ggccgctctt tctcttattt gctgaatgat agacatagta
     2581 tattgtaggg ttttttgaaa ggatggcaaa aatagttgaa aaatgcttat gaacaaattg
     2641 tggaaaacag ggaaaggcag taccatcctg atcccggtga aggaattctg gctgaaccgg
     2701 caaaaaacag attgatttcc gggtgatcac gtgaaacaac cgaaaaagag tggaactcta
     2761 accaacatct ctacgtgtat aaaccatctt tttcagtgga aaacagggtt ttgcgccagg
     2821 gatttctgtg aaaggaatgc gggtagattt tttattttgc cagcccttcg tttttatact
     2881 gaacaaacaa ccacttgttc cgttttttat tatgctggtg tgcttaaata gttatccaga
     2941 gcccggaaag gctgaccgtt tgttaatgaa ggggaaatcg tttacccggg ggcttttccg
     3001 gatgttgaaa ggctgttaat taaaggggat aagaaaatag ttggttcaaa acaatattta
     3061 aatatcaggg ttttccacta atccccatcc ctaatagtaa tagctctatt taaataaata
     3121 atgtattatt atatattatg gcagttatga acatggatca ggctaaaatg cagattaccc
     3181 ggaaagggtt cctggacata ccggttaatc cggagctgga tctttttgaa gaaattgcca
     3241 ggttaaaaaa agagaagaat gccgtgcttc tggcgcacta ttaccaggat gcggatattc
     3301 aggatgtggc tgattttatc ggggatagcc tgggactggc acaggaagcc gcgcggacgc
     3361 gggcggacat tatcgttttt gccggggtcc attttatggc ggaaaccgca aaaatcctga
     3421 atcccgataa aaaggtattg cttccggatc tgaatgccgg atgttccctg gccgattcgg
     3481 cgccggcccc tcttttccgg agtttcaggg agaaataccc ggaccatctg gtcatttcct
     3541 atatcaattg cagcgcagag atcaaggccc tcagtgatat catctgcact tcttccaatg
     3601 cggaaaagat catagacagc cttccggcag atcagaagat tatttttgct cccgataaaa
     3661 acctgggagc gtggctgatc aagaaaacgg gcaggaacat ggtgctttgg aatggagcct
     3721 gcatggttca tgaaatattc agtcagaaaa aaattactgc gctgaaggcg aggcatccgg
     3781 aggcaaagtt cattgcccac ccggaatgcg aggaacccgt tttgcgcatg gcggatttta
     3841 tcggttcgac caccgcctta ttaaaattca cgcagaaaga cccgtctaaa gaatttattg
     3901 taggaacgga atccggcatt ttgcaccaga tgatgctggc ttctccggaa aaaacattta
     3961 ttcccgctcc gccggagacg aactgtgcct gtaatgactg cccgcacatg aaactgaata
     4021 cgctggaaaa gctctatctg tgtatggaat atgaggcgcc ggagatccgg atggaaagaa
     4081 gcctgctgga ggctgcccgg aaaccgatcg accggatgct ggagatcagc cggcaatacg
     4141 ggctctgatc atcgtcccat ataaaccatc aggatttgta tatccgtcgg atttaccccg
     4201 ctgatccggc tggcctggcc cagggtcctg ggctttattt ttttgaattt aatcagggct
     4261 tccatggaaa gcgcagaaat ccggtcgtaa tcgaatgttt ccgggatcga aagttgttca
     4321 agctgtgaaa tccgttttac cagttctttc tcctttttga tataaacttc atacttcatc
     4381 tggatttcgg cctgctccag gctctccgga gggaaagatt ccagtttttc ggccagggca
     4441 ggaatttgtc ccttaagctg agtaatgccg atcaggggtc ttagaatgag ggtggatgcc
     4501 ttttgttttt gaaccagcgg ggaaagattc atttctccca ggaaaacctc ggcttcctgt
     4561 ggggatactg aaaaattatt taaaataaag gaaatttcat tcacctcatt tttcttgata
     4621 tttactgctt ttagtctttc ttcagaggca aggcccaggg aatggctttt ttctgtcagc
     4681 cgcagatccg cgttatcctg tcgcagcaga gtacggaatt cagcccggga agtaaacatg
     4741 cggtagggtt cttccgtacc cttactgatg aggtcatcga tcaaaactcc gatataggct
     4801 tcgctccggc ttaaaatgac cggctccagg tggtttattt tccggtgcgc atttattccg
     4861 gccattaaac cctggcaggc agcctcttca tatccggttg tgccgttgat ctgacccgcc
     4921 agaaagaggt tatttaatat tttcgtctcc aggctgtgcc ccagctgggt cggcgggaaa
     4981 taatcgtatt caatggcata tccgggccgg aaagccttga aatgctcaaa accctttatc
     5041 atacgaagtg cttcgtattg aacttcctcc ggcagggaag tgctgaagcc atttacgtag
     5101 atctcaatgg tgttccatcc ctccggctcg ataaagagct ggtgcctctc cttgtctgca
     5161 aaacggttga gcttgtcctc gatgctgggg caataccggg gtccggtgcc atcaattctt
     5221 cctgtaaaca tcgggctccg gtcaaacccc gttttcagga tttcgtgaac ggcgggattg
     5281 gtataggtga tccagcagct tctttgccgg gccggcaggg gaatattcaa ataggaaaag
     5341 ccaaccggct tttcgtcgcc gggctgttcc tccattttac tataatccag gcttctgccg
     5401 tcaatccggg gaggtgttcc ggtttttaac cggttacttt caaagccttc gctccgcaat
     5461 tgttccgtga tgccggtgga tgctttctcg gacatcctcc cgccgccgaa ttgtttt
//
LOCUS       DASYCU010000229         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_775636, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000229 DASYCU010000000
VERSION     DASYCU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775636"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VGM24_04695"
     CDS             <1..1002
                     /locus_tag="VGM24_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4154696.1"
                     /translation="NEIPEVNIRVEGNRSFYLPGKPLRYAVSVNQQGNTKIPDPADLY
                     VSADFTEKADAALNRPGAGESISTGRILTQTLDCKSCHRENEKSVGPSFMQVSEKYRE
                     DKNAFSYLTEKVTKGGSGVWGEVAMSAHPNILQTDLKQILQYILTLSKKEGAQKSLPA
                     SGIIRPPKETKPKEGLLLSAVYSSRGSGANIKALSGRAVVLLRSSWLLFTGRENKTGF
                     TGLHQNDRDELRPGQEGGWFALDSIDLSGVQAVDIHAGSEKSGSFSIRVVEDKPEGKL
                     IGSGRIGSADSAKEGSFSTRIPLQAVPGKDFHTLYFIFSPADIKHTPVINIEGVEFR"
     gene            complement(1042..3069)
                     /locus_tag="VGM24_04700"
     CDS             complement(1042..3069)
                     /locus_tag="VGM24_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEY4154697.1"
                     /translation="MQIRNIRILEENKTRYFWPLDETAGDLCYDRISHKLAKVKNPEW
                     LKPKYQKWEMIGSFTINGYAGVAYDPIQDKLYIAGSDSLAVYTLKNERHMDWSACQHQ
                     DLLLGHQFIYDTISRRLMDIYIDLKKPLVYDFNNQRWDLTLPTGRVNVTGFWHANKFI
                     SSRDTSLYTFGGYGYLRYKNEVQRYHFSTKKWDSLRPGGDYFSPRYLSALGAGPEGKF
                     VYILGGYGSQTGDQMLDPRYYYDLLRYDVQRNSFKKLFSFKPQTTPFTFANSLVIDSN
                     AFYGLVFSNDSFNSNLQLIRGSLKDSSFRLLGSAIPYNFHDIQSFADLYYSPVSNKLI
                     AVTLFYTPEEAKGKNTQVKIYSVDFPPEPVDAPGVSQAGTKNRNGFIILLGGILLAGA
                     ALIFLRKKKRGTEPENRIRQPLTTEELPGISNMPFRQSHPDPVPHSSIYLFGQFQVFD
                     REGNDITRAFTPLLKELFLIICVYTFRNGRGISSEGLNEILWHDKSEKDAKNNRSVNI
                     AKLKNILERTGNCVINKESGYWQLMNPGEDVFIDYKNYVSLSGSVSSTDREHIGTVLN
                     IIKRGAFLTQTEYNWLDNIKSEVSNSVIDICLGFIRTQDLARDPEFIIEITNYIFYFD
                     QLNEDALICKCKCLILLKRHTLANNCYLRFIKDYKDIYGEEFSRSFHEVIT"
BASE COUNT          839 a          795 c          824 g          811 t
ORIGIN      
        1 aatgaaattc ccgaagttaa tatcagggtg gaaggaaacc gctccttcta cctgcccggc
       61 aaaccgctcc ggtatgctgt ctccgtcaat cagcagggaa atacaaaaat accggatccc
      121 gcagacctct acgtgtccgc ggattttaca gaaaaagcgg atgccgcgct gaaccggccc
      181 ggcgcaggtg aaagcatcag taccggccgg atccttacgc agacgctgga ttgcaaaagc
      241 tgccaccggg aaaatgaaaa atcggtcggg ccttccttta tgcaggtttc cgaaaaatac
      301 cgggaagata aaaatgcctt cagttacctt acggaaaagg taacaaaagg gggaagcggc
      361 gtatggggag aggtggccat gtcggcgcat cctaacattt tacaaacaga cctgaaacag
      421 atcctccaat acatcctgac gctttccaaa aaagaaggag cacaaaaatc cctgcctgct
      481 tccggaatca tcaggccccc gaaagaaaca aaaccaaagg agggcctgct tttatcggcg
      541 gtatattcca gccggggttc cggcgccaac ataaaagcac tgagcggccg ggcagtcgtt
      601 ttgcttcgca gcagctggct tttatttacc ggcagggaaa acaaaacagg atttaccggg
      661 ctgcatcaga atgaccggga cgagcttcgc ccggggcagg aaggaggatg gttcgcgctg
      721 gactccattg acctgagcgg cgtgcaggct gttgacatcc atgcaggcag cgaaaaatct
      781 ggtagctttt ccatccgggt cgtggaggat aagccggaag ggaaactgat cggaagcggc
      841 cggatcggat ctgcggactc cgcgaaagaa ggttctttca gcacccggat cccgctgcaa
      901 gcggttcccg gaaaggattt tcatacttta tatttcattt ttagtccggc ggacatcaaa
      961 catactccgg ttataaatat tgaaggagtt gaattccggt aactaattca gcaggatggt
     1021 ccggtttaac cggcagcccc tttacgtgat cacttcatgg aatgacctgg agaattcttc
     1081 gccgtaaata tccttgtaat ccttgatgaa ccgcaggtaa cagttattgg caagggtatg
     1141 ccgcttaagc aggatcaggc acttgcattt acagatcagg gcgtcttcat tcaactggtc
     1201 gaaatagaaa atataattgg tgatctcaat gatgaattcg ggatcccttg ccagatcctg
     1261 ggtccggata aaacccaggc agatatcaat gaccgaattg gacacctccg atttaatatt
     1321 gtcgagccag ttgtattctg tctgggtcag aaatgcgccc cgcttaatga tattaagcac
     1381 ggtaccgata tgctcccggt ccgtactcga aacggacccg gacaaggaga cgtaattttt
     1441 ataatcgata aatacgtctt ccccgggatt catcaattgc cagtagccgg attccttatt
     1501 gatcacacaa ttacccgttc tttccaggat gttctttaat ttggcaatat tgaccgagcg
     1561 gttatttttt gcatcctttt cggatttatc gtgccatagg atctcattca ggccttccga
     1621 tgatatccct ctcccgttcc tgaatgtata tacacagatg atcaggaata attccttcag
     1681 cagcggagtg aacgcccggg ttatatcgtt tccctcccgg tcaaaaacct ggaactgtcc
     1741 aaacaggtat atggaggagt gaggcaccgg atccgggtgg ctttgcctga atggcatatt
     1801 gctgattccc ggcaattcct ccgtggtcag cggttgccgg atccggtttt ccggttccgt
     1861 ccctctcttc tttttccgga gaaagatcag tgcagctcct gccagcagga ttcctccaag
     1921 gagaataatg aacccgttcc tgttttttgt tccggcctgg ctgaccccgg gagcatccac
     1981 tggctccgga ggaaaatcca ccgagtaaat tttcacctgg gtattttttc ccttcgcttc
     2041 ctccggcgtg taaaacaaag tgacggcgat cagcttattg ctgacgggag aataatacag
     2101 gtcggcgaag gactgtatat cgtgaaagtt ataggggatg gcacttccca gcaaacggaa
     2161 gctggaatcc ttcagcgatc cgcggatgag ctgcaggttg gaattaaagc tgtcgttcga
     2221 aaagaccagc ccgtaaaacg cattggaatc aatgaccaga ctgttggcaa aggtgaatgg
     2281 cgtcgtctgc ggtttaaagc tgaaaagttt tttgaaggaa ttcctttgca catcatagcg
     2341 gagcaggtca taataatacc ggggatccag catctggtcg ccggtctggc tcccatatcc
     2401 gcccagtatg taaacgaact tcccttccgg tccggcgccg agggcagaca ggtagcgggg
     2461 agagaaatag tcccccccgg gtctaaggct gtcccatttt tttgtagaaa aatgatatcg
     2521 ctgcacctca ttcttatacc gcaggtatcc ataaccgcca aatgtataca gagaggtgtc
     2581 ccgggaagaa atgaatttat tggcatgcca gaaaccggta acatttaccc ttccggttgg
     2641 cagggtaagg tcccagcgct gattgttgaa gtcataaacc aggggctttt tcagatctat
     2701 ataaatatcc atgagcctcc ggctgatcgt atcgtaaatg aactgatgcc cgagcagcag
     2761 atcctgatgc tggcaggccg accagtccat atgcctttca ttttttaaag tatatacggc
     2821 gagggaatcc gatcccgcaa tatagagttt atcctgaatg gggtcatagg cgacaccggc
     2881 atacccgttg atggtaaaag atccgatcat ctcccatttt tggtatttgg gcttcagcca
     2941 ttccggattt ttcactttgg ccagcttatg gctgatccgg tcataacaaa gatctccggc
     3001 tgtttcatcg aggggccaga aataccgggt cttattctct tccagtatgc ggatattccg
     3061 gatctgcatg ggaggaagat cgcgggtctt gaatttttgg aaatcgtttg ccccgaataa
     3121 gatcttgaaa caattctcgc tgacaggcag tttacccgtt ccgatcagcc tgccattgac
     3181 aaagcattgg atcgaatgat ttccgggatc gcattacagt cggaacaggt tccattgttt
     3241 gtagaggcgc aggctgtcaa tagtaaagc
//
LOCUS       DASYCU010000230         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_339691, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000230 DASYCU010000000
VERSION     DASYCU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339691"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGM24_04705"
     CDS             <1..213
                     /locus_tag="VGM24_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY4154698.1"
                     /translation="TEYMSNPSKIEYMIFPRVDALSEVVWSPRTARNWDDFQKRLPFQ
                     LKRYDLWHTHYFGDSSQYGEQAKSKN"
     gene            complement(231..>1504)
                     /locus_tag="VGM24_04710"
     CDS             complement(231..>1504)
                     /locus_tag="VGM24_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEY4154699.1"
                     /translation="LLGAISAILSCITGYILSDVDSYEEDLKNWHQWMGISTAVLSLI
                     VYYFSRRPEWGRWLNSLSLLMLAGIVITGHLGGSLTHGSDYFSKPIAELLGAPEKVEP
                     VFKKPIPDIQEAVVYRDLIHPIFQARCAACHGEKKQRGKLRLDDPSGILEGGKSGKAI
                     VPGKPDLGELIKRITLPPESKDHMPPKGKPQLTDNEVALVKWWLADSADFEKKVHRMN
                     QPTAIKPVLVALQEGVDTQSVKTAEVPEKPVDPAPEQVMNILRSRGIVVLPVAKNSNY
                     LSVSFVIAPVEDSSLALLEKLADQLVWLNMSGSPLSDKGLQRIGRLPQLTKLYLNGTQ
                     VTDSGLSFLKPLKRLQYLNLVHTGISQKGLEKLAGLKGLKNIYCYQTKIHSGSWPYLS
                     QVFPQAKLDTGGYQVPTFVTDTSIYKRESKK"
BASE COUNT          351 a          386 c          368 g          399 t
ORIGIN      
        1 actgaataca tgtccaatcc gtccaaaata gaatacatga tcttcccccg ggtagacgcg
       61 ctgagtgagg tggtatggag ccccaggacg gccaggaact gggatgactt tcagaagaga
      121 ttgccgttcc agctaaaacg gtatgatctc tggcataccc attatttcgg ggacagttcg
      181 cagtacgggg agcaggcaaa aagtaagaat taagaaagag atgcgtttct ttattttttg
      241 ctttctcttt tgtaaatgct ggtatccgtt acgaaagtcg ggacctggta cccgccggta
      301 tccagttttg cctgcggaaa tacctgcgag agatagggcc agctgccgga gtgaattttt
      361 gtctggtaac aataaatatt ttttagccct ttcagtccgg ccagcttttc aaggcccttc
      421 tggctgatgc cggtatgcac gagattcagg tattgcaatc ttttaagcgg ttttaaaaag
      481 gataaaccgc tgtcggtaac ctgggtgccg ttcaggtata attttgttaa ctgcggtaac
      541 ctcccgattc gttgcaggcc cttatcagat aatggagaac cgctcatgtt cagccagaca
      601 agctggtcgg ccaacttttc caacaaggcc aggctgctgt cttcaaccgg agcgatcaca
      661 aagcttacgg aaagataatt gctgtttttc gctacgggca aaaccacgat gccccggctt
      721 cgcaggatat tcatcacttg ctccggggca ggatccaccg gtttttccgg aacctccgcg
      781 gttttaaccg actgggtatc tacgccctcc tgcaaggcga ccagtaccgg tttgatggcg
      841 gtcggctggt tcatccggtg caccttcttt tcaaaatcag cgctgtcggc cagccaccat
      901 tttaccaaag ccacttcatt gtctgtaagt tgcggtttac cctttggagg catatggtcc
      961 ttgctttccg gcggaagggt gattcgtttg atcaattcgc ccagatccgg tttccccggt
     1021 acgatggcct tccccgattt tccgccttcc agaattccgc tgggatcatc cagccttaat
     1081 tttccccttt gttttttttc tccatgacag gcggcgcatc ttgcctgaaa tatcggatga
     1141 atgaggtccc ggtaaacaac cgcttcctga atatccggaa tgggtttctt aaaaacgggt
     1201 tcaacctttt ccggagcgcc cagcagttca gctatcggtt tggaaaaata atctgatccg
     1261 tgcgtaagcg atccgcccag atgacccgtt atcacaatgc cggccagcat cagcagggaa
     1321 agggaattca gccacctgcc ccattccggt cttctggaaa aataatacac gatcagcgag
     1381 agtacggcgg tgctgattcc catccactgg tgccagttct ttaaatcttc ttcgtaactg
     1441 tccacgtcgg aaagaatata ccccgtaatg caggatagaa tagcgctgat cgcgccgagc
     1501 agaa
//
LOCUS       DASYCU010000231         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2224994, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000231 DASYCU010000000
VERSION     DASYCU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224994"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1114)
                     /locus_tag="VGM24_04715"
     CDS             complement(56..1114)
                     /locus_tag="VGM24_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY4154700.1"
                     /translation="MFDQLQQLFKRFPDFLWNLVIILLAIGIGLVCRSLAKAIVGKEK
                     PLDTGFFFWHSFIRRMSRPLSFYLPFLALYLMLPVMRISPSFSSSLQKLADILLFITF
                     AGILIGIVRIGEDYVYYTYDIKKGDNLRERKIRTQLQFIRRLVMILIVIITLCFVLMR
                     FDSLRRIGSGLLAGVGVSGIIIGFAAQKSLGSFMAGFQIAFTQPIRIDDVLVVEGEWG
                     RVEEITLTYVVLRIWDKRSLVIPINYFIEKPFQNWTRTGADLIGTIFIYLDYTVPVDS
                     LRVELDRLLSLTPLWNRETKAVQVTNLTDKTIEVRALVSANNSSDTFDLRCYIREGLL
                     KFVKLNYPDSLPLMRIKN"
     gene            complement(1139..>2671)
                     /locus_tag="VGM24_04720"
     CDS             complement(1139..>2671)
                     /locus_tag="VGM24_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154701.1"
                     /translation="HEGKIKLLDYFKKEISSRSYADWVFDEKMKFLRSHMNGELNNLM
                     RWLDPLIGKEGSRPATEIKEAMAVLMSAFPVYRIYPDRLPLSETDRSRLKEAFEKAFM
                     KASGLKEVLEGLQRFFQKEPAGDEYLYFLRRWAQYTAPLAAKGTEDTVFYNYDPLISH
                     NEVGDSPDHTPVTAGRFHELMARRLQDAGLSLNASSTHDSKRGEDNRLRINLISRYAD
                     EWIAWVEKWKRINGPFRRMHHEQPAPSAHDEYLIYQALVGAVPENGRISLAFRKRFEK
                     YLIKALKEEKQETGWEYPDTDYEKYCLEFLNHILSPAHDFLSGFLPFASRIVEQADIF
                     SLGQTLIKLTAPGIPDIYQGAECRDLSLVDPDNRLPVDYAGRISMMNAILSKEEEGDI
                     PILDYVKQHAGSGAQKMFLIRKILHTRKKFPDLFNRGRYLPLVSTSNLIGYVRENEDR
                     QLLVLVPLPSASEETVPLQDSHIHGRWTCLFSNQEREIDDLTGSGALLEKFPVAALIR
                     QK"
BASE COUNT          652 a          697 c          661 g          661 t
ORIGIN      
        1 ttagcttatt gataaaagat aggagatccc ggatttttgg caacaatgta tcgtgtcaat
       61 tcttaattct catcaaaggc agcgaatccg ggtaattgag cttcacgaac ttcaaaagcc
      121 cttcccgtat atagcagcgc aggtcgaagg tgtcggagga attattggcg ctgaccaggg
      181 cgcgcacttc aatggttttg tccgtcaaat tggttacctg tacggctttg gtttccctgt
      241 tccaaagcgg cgtcagcgaa agtaagcggt cgagttcgac gcgcagggaa tccaccggca
      301 cggtatagtc caggtaaatg aaaatcgtgc cgatgagatc ggctcctgtc cgggtccagt
      361 tctgaaaggg tttttcaata aaataattaa tagggatcac gaggcttcgt ttgtcccaga
      421 tcctcagcac cacataggtg agcgtaattt cctccaccct cccccattcc ccttccacga
      481 ccagcacatc gtcgatccgg atcggctggg taaatgcaat ctggaaaccg gccataaagc
      541 tgcctaagga tttttgtgcg gcaaatccga tgattatccc gctcaccccc accccggcaa
      601 gcagacccga accgattctt cggagactgt caaaacgcat cagcacaaaa caaagcgtaa
      661 taatcacgat caggatcatg accagccgcc ggatgaactg caattgggtg cggatcttcc
      721 gttcgcgcag gttgtcgcct ttttttatgt cgtaggtata ataaacataa tcttccccta
      781 tccggacgat accgatgaga atgcccgcga acgtgatgaa gagtaagata tcggccaatt
      841 tctgaagact gctgctgaac gaagggctta tgcgcataac cggaagcatc aggtaaagcg
      901 ccaggaaagg gagataaaaa ctgagcggac gactcatccg ccggataaaa gagtgccaga
      961 aaaagaaccc ggtatccagc ggcttttcct tcccgacgat ggctttcgcc agggaacggc
     1021 agaccagccc gataccgatg gcgagcagaa tgatgaccag gttccacaaa aagtccggga
     1081 atcttttgaa tagttgttgc aattgatcga acatgcgcaa gccctttctg taatttaatc
     1141 atttttgccg gatcagggcg gccaccggaa atttttccag caacgcaccg gaaccggtta
     1201 aatcatctat ctccctttcc tgattgctga acaggcaggt ccaacgccca tgtatatgcg
     1261 aatcctggag gggaacggtc tcttccgatg cggagggcag gggaaccagc acgagcagct
     1321 gccggtcttc attctcccgg acatagccga tcaggtttga agtgctgacc aggggaagat
     1381 aacggccccg gttaaacaga tccgggaatt tctttcgggt atgtaaaatt ttccggatca
     1441 ggaacatttt ttgtgccccg gatccggcat gttgctttac gtaatccaaa attgggatat
     1501 ccccttcttc ttctttactc agaatggcat tcatcatact gatccggccg gcataatcca
     1561 ccggcagccg gttatcgggg tccaccaggg aaaggtcccg gcattcggcg ccctgataga
     1621 tgtccggaat tccgggtgct gtaagtttaa tcagggtttg accaagagaa aaaatgtccg
     1681 cctgctcaac gatcctgctc gcaaaaggaa gaaaaccgga caggaaatcg tgtgcggggg
     1741 aaagaatatg attcagaaat tcaaggcaat atttttcata gtccgtatcg ggatactccc
     1801 agcccgtttc ctgtttttct tccttcaatg ccttgatcag gtatttttcg aatcgtttcc
     1861 ggaaagccag actgattcgc ccgttttccg ggacggctcc caccagggcc tgataaatca
     1921 ggtattcatc atgggccgac ggagcgggct gctcatgatg catccggcgg aaagggccgt
     1981 tgatccgttt ccatttttca acccaggcga tccactcgtc agcataccgg ctgatcaggt
     2041 taatgcgcag gcggttgtct tctccgcgct tgctgtcgtg tgtggaactg gcattgaggc
     2101 tcaatccggc atcctgaagg cgcctggcca tgagctcatg aaaccttccg gccgtaaccg
     2161 gagtatggtc cggcgaatct cccacttcat tatggctgat gaggggatca taattgtaaa
     2221 aaacagtatc ctcggtgcct ttggcggcaa gcggagccgt gtactgcgcc catcttctca
     2281 ggaaatataa atattcgtct cccgccggtt ctttctgaaa aaaccgttgc agcccttcca
     2341 ggacttcttt taaaccggac gctttcataa acgctttttc aaaagcttcc ttcagccggc
     2401 ttcggtccgt ttccgatagc ggcaaccggt ccggatagat ccggtaaacg gggaatgcgg
     2461 acatcaatac cgccatcgcc tctttgattt ccgtggccgg ccgcgagcct tctttcccga
     2521 tcagcgggtc cagccatcgc atgaggttgt tcagttctcc attcatatgc gaccggagga
     2581 atttcatttt ctcatcaaag acccaatccg cataggacct gctgctgatc tcttttttga
     2641 aataatccag cagttttatt ttaccctcgt g
//
LOCUS       DASYCU010000232         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_196967, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000232 DASYCU010000000
VERSION     DASYCU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196967"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VGM24_04725"
     CDS             <1..991
                     /locus_tag="VGM24_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY4154702.1"
                     /translation="EGKSISGIATVIWLDMADALIQTCMHLAKNIYLDSNENDRKSSL
                     IQTREYRFIEECKKKYPLHQYQRAAPILKDLRSVKTAEEVEVIQKAIDITGNAFHRVL
                     KFMRPGVFEFEVEAEIQHSFLSQRATGPAYGIILASGERACTLHYVSNNQECKDGEMI
                     LMDFGAEYGGYNADLTRSIPVNGKFTRRQKEVYNACLHLHQFAKSLLRPGTIITEYHQ
                     LVGKEADRVFQKIGLLSKSDIKNASRESPAYSKYLYHGISHHLGIDVHDLGTRTEPLR
                     AGMVLTVEPGIYIKEEKLGIRIENNVWLTKNGNRDLMKDVPITVDEIEAAMKP"
     gene            1022..1786
                     /locus_tag="VGM24_04730"
     CDS             1022..1786
                     /locus_tag="VGM24_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY4154703.1"
                     /translation="MKQIPNFFTLLNLFFGCIALICILQNGISIMYSPDGIQYVDIPE
                     KIWLAPLFMALAAVADFFDGFVARLLKVESPLGRELDSLADLVSFGVAPGMILYQFLR
                     MSFMRQADGADVSMIWLLPVLILPCAAALRLARFNLDLTQSHSFRGIPVPAVGLLIAS
                     FPLIYWTKNSTRILDILLNKWFIYGLILLLSYGMISRFPVMAMKFGKGAAGQNLPRLI
                     LLLIALISLFILHWLAVPVIFLFYIVLSLIFKNRII"
     gene            1783..2028
                     /gene="purS"
                     /locus_tag="VGM24_04735"
     CDS             1783..2028
                     /gene="purS"
                     /locus_tag="VGM24_04735"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY4154704.1"
                     /translation="MNFLVQVKVMPLKELLDPQGKAVMGGLRNLGLESVNDVRIGKTI
                     QLEVEAQSAEKAKQIAEEAGKRLLANPVMEYFEVTVN"
     gene            2051..2716
                     /gene="rsmI"
                     /locus_tag="VGM24_04740"
     CDS             2051..2716
                     /gene="rsmI"
                     /locus_tag="VGM24_04740"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY4154705.1"
                     /translation="MLYLVPSPIGNLQDISLRALETLKKADLILAEDTRTSLKLLNHY
                     QIIKPLTPYHQHNEHKVLHHLVDQLLQGKIMALLTDAGTPGISDPAFLLVRECIQVNV
                     RVECLPGATAFVPALVNSGLPMNRFCFEGFLPLKKGRQSLLKKLALEPRTMVFYESPL
                     RLLKTLQEFILYFGAGRKCCVSRELTKLYEENIRGTLQEVAGHFQEKGVKGELVLVLE
                     GTG"
     gene            2749..>3731
                     /locus_tag="VGM24_04745"
     CDS             2749..>3731
                     /locus_tag="VGM24_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEY4154706.1"
                     /translation="MKQSLVYFRRILRFAGCMIVAQGMVVFCLAQPDRWQQQVKYFLD
                     IDMNVHTNRFSGKEHLEYSNHSPDTLRKVFYHLYWNAFQPNSMMDNRSRVLGQNLING
                     EPDWDERVKDRILHLTPQEIGYQKIISLKMNGAPQDFKMEETILEVDLGKPILPHSTV
                     SFDIVFEAQVPLQIRRSGRDNPVTKVRYSMSQWYPMICEYDYEGWHATPYVAREFYGV
                     WGDYDVKINIDRSYIIGATGYLQNPNQIGYGYESPGTRVTRPAGDKLSWHFIAPDVHD
                     FVWAADPEFKHVSKKIRNDLTIHVLYKVTNASEKSWLTILDQVSRAMPFIER"
BASE COUNT          961 a          906 c          959 g          905 t
ORIGIN      
        1 ggaggggaaa agcatttccg gaatagccac ggtgatctgg ctggatatgg cggacgccct
       61 gatccagacc tgcatgcacc tggcaaaaaa tatttacctc gattcaaatg agaacgaccg
      121 taaaagcagt ctcattcaaa cccgggaata ccgcttcatc gaagaatgca aaaagaaata
      181 tccgctgcat cagtatcagc gggcggcccc gatcctgaag gatctccgtt cggtgaaaac
      241 ggcggaagag gtggaagtaa tacagaaagc catcgacatt accgggaacg catttcaccg
      301 ggtgctgaaa tttatgcgcc cgggtgtttt tgaatttgag gtggaggcgg agatccagca
      361 ctcctttctt tcgcaaagag ctaccggtcc ggcatacgga atcatactcg cttccggtga
      421 gcgggcctgc acccttcact atgtatcgaa caaccaggaa tgtaaagacg gggagatgat
      481 cctgatggat ttcggggcgg aatacggggg ctataatgcc gacctcacgc gcagcattcc
      541 ggtgaacgga aagtttacca ggcgtcagaa agaagtgtat aatgcctgcc tccatttgca
      601 ccagtttgcc aaaagcctgc tcaggccggg aaccatcatc accgaatacc atcagctggt
      661 gggaaaggaa gccgaccggg tatttcaaaa aatcggattg ctcagcaaat ccgatataaa
      721 gaatgccagc cgcgaaagcc cggcctattc gaaatatttg taccacggca tttcccatca
      781 tttgggaatt gacgtgcatg acctgggtac gcgaacggaa cccctgaggg caggtatggt
      841 gctaaccgta gagcccggca tctatattaa agaagaaaaa ctgggcatac ggatcgaaaa
      901 taatgtctgg cttaccaaaa atgggaacag ggacctgatg aaagacgttc cgatcaccgt
      961 cgatgaaatt gaagccgcca tgaaaccctg agcaggaacc ggacacaaac cagcaccatt
     1021 catgaaacag atcccgaact tttttacctt attgaattta ttttttggtt gcatcgccct
     1081 gatctgcatt ttgcaaaacg ggatcagcat tatgtattcc ccggatggca tacagtacgt
     1141 ggatatcccg gaaaagatat ggctcgcgcc actctttatg gcccttgccg ccgtagcgga
     1201 tttttttgat ggttttgttg cgcggctgct taaagtggaa tccccgctgg ggcgggaact
     1261 ggattccctg gcagacctgg tgagttttgg cgtggctccg ggaatgatcc tttatcagtt
     1321 tttaagaatg agctttatgc ggcaggcaga tggcgcggat gtttccatga tctggctcct
     1381 gccggtactg atcctgccct gcgcggctgc cctgcgcctt gcccgcttta acctggacct
     1441 tacccaatcg cattcattcc ggggcattcc cgtgccggcc gtgggtctgc tgatcgcctc
     1501 ttttccttta atctactgga caaagaacag cacccggatc ctcgatatcc tgctgaataa
     1561 atggtttatt tacggcctca tccttttatt aagctatggc atgatcagcc ggttcccggt
     1621 aatggcaatg aaattcggca aaggagcagc aggccagaat cttccccgcc tgatcctgct
     1681 cctgatcgcg ttgatttcac ttttcatttt acactggctg gcagtaccgg tcatcttcct
     1741 tttttacatt gtattatctt tgatcttcaa aaacaggatc atatgaattt tctggtgcag
     1801 gttaaggtga tgcctttgaa agaattactg gatccccagg gaaaagccgt aatgggagga
     1861 ctccgtaatc ttggtttgga atcggtgaat gatgtacgga ttggaaagac catccagctg
     1921 gaggtggagg cacagtccgc cgaaaaagca aaacagattg ccgaagaagc aggcaaaaga
     1981 ctcctcgcta acccggtgat ggaatacttt gaagttactg tgaattaata cgaagatcgg
     2041 tttaaccacc atgctctacc tcgttccatc acccatcggc aacctgcagg acattagttt
     2101 acgggctctg gaaaccctta aaaaggccga tctgattctg gcagaggaca cgcgcacttc
     2161 gctcaagctg ctgaatcatt atcagatcat caaaccttta acgccttatc atcagcacaa
     2221 tgagcacaag gtgctgcatc acctggtgga tcagctgttg caggggaaga tcatggctct
     2281 cctgaccgac gcgggaacgc ccgggatttc tgatccggct tttttgctgg tgcgggaatg
     2341 catccaggtg aacgtccggg tggaatgcct tccgggcgcc acggcctttg ttccggcgct
     2401 ggtgaacagc ggactgccca tgaaccggtt ttgttttgaa ggatttcttc ctttgaaaaa
     2461 ggggagacaa agcctgctga agaaactggc tcttgagcca aggacaatgg ttttttatga
     2521 atccccgctt cggctgctga agacattgca ggaattcatt ttatatttcg gcgccggccg
     2581 gaaatgttgt gtctcccggg aacttacaaa actatacgaa gaaaatatcc gcggcaccct
     2641 gcaggaagta gccggccact tccaggaaaa aggagtgaag ggagagcttg tcctcgtact
     2701 ggaaggcacg ggatagcctt atctattttt catctgatga tttgagttat gaaacaatcg
     2761 cttgtttatt tcagaaggat tttacggttt gccggctgta tgatcgtggc ccagggaatg
     2821 gttgtctttt gcctggccca gcctgaccgc tggcagcagc aggtaaaata tttcctggac
     2881 attgacatga atgtgcacac gaaccggttc agcggaaaag agcatcttga atacagcaat
     2941 cattctcccg ataccttacg aaaggttttt tatcatctgt actggaatgc ttttcagccg
     3001 aacagcatga tggacaaccg gagccgggtg ctggggcaaa acctgattaa cggagagccg
     3061 gactgggatg aacgggtcaa agaccggata ctgcatctga ctcctcagga aatcggatat
     3121 cagaagatca tttcactgaa aatgaacggt gcgccccagg attttaaaat ggaagaaacc
     3181 attctcgaag tggatcttgg caaacccatt cttccccata gtacggtgag ctttgatatc
     3241 gtgtttgaag cccaggtccc attgcagatc cgcagaagcg gccgtgataa ccccgttacc
     3301 aaagtacgat actccatgag tcagtggtac ccgatgatct gtgaatatga ttacgaagga
     3361 tggcatgcca cgccttatgt ggccagggag ttctacggag tgtggggcga ttatgatgtg
     3421 aagatcaata ttgacagatc ctatataatc ggagcaaccg gctatttgca aaaccccaac
     3481 cagatcgggt acggctatga atccccgggc acccgggtga cgcgtcccgc aggggataag
     3541 ctcagctggc atttcattgc cccggacgtg catgattttg tatgggccgc cgatccggaa
     3601 tttaaacacg tcagtaagaa aatccggaac gacctgacca ttcacgttct ttacaaagtc
     3661 acgaatgctt ctgaaaagag ctggctgacc atcctggacc aggtcagccg cgccatgcct
     3721 ttcattgaac g
//
LOCUS       DASYCU010000233         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4977834, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000233 DASYCU010000000
VERSION     DASYCU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4977834"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VGM24_04750"
     CDS             complement(<1..348)
                     /locus_tag="VGM24_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY4154707.1"
                     /translation="MQIPKFSASPHTFHSDLKKRINTYFDQAGKSMTGNFHLYLKAVV
                     LGLSFIGLYIHLVFFTPVTLLAIGECVLLGGVISAIGFNVMHDGAHGSFSKRKWVNMM
                     AAFSLNVLGGNSFI"
     gene            complement(481..1224)
                     /gene="bshB1"
                     /locus_tag="VGM24_04755"
     CDS             complement(481..1224)
                     /gene="bshB1"
                     /locus_tag="VGM24_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEY4154708.1"
                     /translation="MIRKINVLAIAVHPDDVELCCAGTLMMEKLHGKTTGVIDLTQGE
                     LGTRGTPALRIQESEAAGKVMGLDIRQNLCMSDGFFQNNEINQRCLIAAIRKYQPEIV
                     LASALSDRHPDHGRAGKLISDSCFLSGLRKVETKDDEGQFQQHWRPKYIFHFIQDRYY
                     SPGFVYDISPVFDRKLDAIRAYSSQFHSQTYAKDEPQTYISSPEFLDSVVGRHQMFGK
                     MIGVRYAEGFITEKMIGIRNLDAFVQMDT"
     gene            1344..>2096
                     /locus_tag="VGM24_04760"
     CDS             1344..>2096
                     /locus_tag="VGM24_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4154709.1"
                     /translation="MAYDVVVLGSGPGGYVAAIRASQLGLKTAIIEKESLGGICLNWG
                     CIPTKALLKTAQVFEDIKHAKDFGIEASGKPDFPAVIKRSRGVADKMSKGVQFLMKKN
                     KIDVFMGFGKFKAKGQLEVTGGDGKTQVVEGRHIIIATGARSRELPNLKQDGKKVIGY
                     REAMSLPEQPKSIIVVGSGAIGVEFAYFYHSMGTRVTIVEFLPRIVPVEDEDISKELE
                     KQYKKNGIEIYVSSEVTSVDTSGSGVKAKVKTA"
BASE COUNT          544 a          510 c          555 g          487 t
ORIGIN      
        1 gataaaactg tttccgccca ggacgttgag ggaaaaagcg gccatcatat tcacccattt
       61 gcgtttgctg aaactgccgt gcgccccgtc gtgcatcacg ttgaacccga tggcagagat
      121 cactccgccg agcagcacgc attccccgat cgccagcagg gtgaccggtg taaaaaagac
      181 cagatgaatg tataatccga taaaagaaag acccagcacc accgccttga ggtagagatg
      241 aaaattgccc gtcatcgact ttcccgcctg atcaaaatag gtgttgatcc tctttttcag
      301 atcggaatgg aaagtatgtg ggctggcgga aaatttaggt atttgcatga atattttctt
      361 ttattgaaaa gcgccacaaa ggtacttcaa tttttaaaga agaccatgtt aaaataagct
      421 cccgacgatc gtattccgct ccggtcctct tccctgccag ataacgcaga aacaccggtt
      481 ttatgtgtcc atttgtacaa atgcatccag attccggatt ccgatcattt tttccgtgat
      541 aaacccttcg gcatagcgca ccccgatcat ctttccgaac atctggtggc ggccgacgac
      601 ggaatcgagg aattcggggc tggatatata ggtctgcggt tcgtcctttg cataggtctg
      661 ggaatgaaac tgggaggaat aggcccggat agcatccagc ttccggtcga agaccgggga
      721 aatatcatag acaaatcccg gagaataata gcggtcctgg ataaaatgga aaatgtattt
      781 gggacgccag tgttgctgga actggccctc atcgtccttg gtctccactt tacgcaggcc
      841 gctcaggaaa cagctgtcgg agatcaattt gcccgcccgt ccatggtcag gatgcctgtc
      901 agacagggcg ctggccagca cgatctccgg ctggtatttg cggatggccg caatcaggca
      961 acgctggttt atttcgttat tctggaagaa accatcgctc atgcagagat tttggcggat
     1021 atccagcccc atcactttgc cggcggcttc ggattcctgg atgcggagcg cgggagtgcc
     1081 ccgggtgccg agttcaccct gggtcaggtc aatcacgccg gtggttttgc catgaagttt
     1141 ttccatcatg agggttccgg cgcaacaaag ctccacgtcg tccggatgaa cggctattgc
     1201 gagaacattt atttttctga tcatgccgca aattaaatta agttgggcag agttcagcct
     1261 ccgggacgac aacctgtcat tatcggattt tcccatagct ttgcaatccg aaattcatca
     1321 attatctttt aaaatttatt tctatggcct atgatgtagt tgtgctcgga agtggtcccg
     1381 gcgggtatgt tgccgccatt cgtgcctctc aattgggttt aaaaacagcg attattgaaa
     1441 aagaaagcct gggcggcatt tgcctgaact ggggttgtat tcccaccaag gccctgctga
     1501 aaaccgccca ggtattcgaa gatatcaagc acgccaagga tttcgggatc gaagccagcg
     1561 gaaagccgga ttttccggca gtcatcaaac gcagccgcgg ggtggccgat aaaatgagca
     1621 agggagttca gttcctgatg aaaaaaaata agatcgacgt gttcatgggt tttggaaaat
     1681 tcaaggccaa gggacagctc gaagtgaccg ggggagacgg aaagacccag gtcgtggaag
     1741 gcaggcacat catcattgcc accggagccc gcagccgcga actccctaat ctgaaacagg
     1801 atggtaaaaa agttatcggt tatcgcgagg ccatgagcct tccggaacaa cccaaaagca
     1861 ttatcgtggt cggcagcggc gcgatcggcg tggagttcgc ttatttctat cacagcatgg
     1921 gcaccagggt gaccatcgtc gaattccttc cccgcatcgt gccggtggaa gatgaggata
     1981 tttccaaaga gctcgagaag cagtacaaga aaaacggcat tgaaatatac gtgagcagcg
     2041 aagtaacgag cgtggatacc agcggcagcg gcgtgaaagc caaagtaaaa acggct
//
LOCUS       DASYCU010000234         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1358829, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000234 DASYCU010000000
VERSION     DASYCU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358829"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /gene="prfB"
                     /locus_tag="VGM24_04765"
     CDS             join(<1..40,42..1073)
                     /gene="prfB"
                     /locus_tag="VGM24_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131330388.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY4154710.1"
                     /translation="DMRSRLSGLRRFLDVANRQIKIDEEKQLTGSPGFWNDNERATGI
                     LKNIKVNEYWVKLYESVGTAIEDFAVLLEFWKAGEATEEETREAYQNALDKMEEAEFK
                     STLNQPEDELPAVLQINSGAGGTESQDWAEMLMRMYRMYGEKQGWSVSELDYQDGEGA
                     GIKSVTLQFEGPFAYGFLKAESGVHRLVRISPFDSNARRHTSFASVYVYPLIDDSIEI
                     EVNAADVEWAFFRSGGAGGQNVNKVETAVRLTHKPSGIIVECQQARTQGENREKAMAM
                     LKSRLYEEEIRKREAIKNASNASKKKIEWGSQIRSYVFHPYKMIKDHRTDFETGNVQP
                     VMDGELDGFIKAYLMSEKEMAN"
     gene            1100..2731
                     /gene="pruA"
                     /locus_tag="VGM24_04770"
     CDS             1100..2731
                     /gene="pruA"
                     /locus_tag="VGM24_04770"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEY4154711.1"
                     /translation="MNLGYFHYAMPSNEPVLNYAPGSAEKISLKKAIREIKAQEHDIP
                     MIIGGKEIRTGNKIKMSPPHEISHTLGYFHRAGREQIEQAVEAALQAKPAWERLNWEN
                     RAAIFLKAADLLAGKHRAYMNAATMIGQSKNPFQSEIDGICELIDFLRFNVHYLSEIY
                     RQQPSSSPGVHNRMEYRPLEGFVLAITPFNFTAIAGNLPSSAALCGNTVVWKPADTQV
                     FSAHFFMRILQEAGLPDGVINLVYPEGTELGSVCFSHRDFAGIHFTGSTAVFNKIWKT
                     IGENIGNYKSYPRIVGETGGKDFVLVHPEADAAVAVTALVRGAFEFQGQKCSAASRAY
                     LPSNLAAEIREKLLAAVKTLRVGTVEDFRNFVNAVIDEKSFDKIRGYIDRAEKDPKAK
                     IIAGGKTDKSKGYFIDPTIIETSDPEYITLKEEIFGPVLTIYVYPEQEFEQTLDLVNR
                     TSPYALTGSIISRNRQAIEQATEKLRQAAGNFYINDKPTGAVVGQQPFGGARGSGTND
                     KAGSILNLYRWLSARTIKETFQPATDHGYPFLGEA"
     gene            2765..3286
                     /gene="pyrR"
                     /locus_tag="VGM24_04775"
     CDS             2765..3286
                     /gene="pyrR"
                     /locus_tag="VGM24_04775"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY4154712.1"
                     /translation="MKTILTEEQLGVTIQRLSHQLLENLLQYDELVIIGIQPRGIFFS
                     DRIVKQINQQIAPRKITYGKLDITFYRDDVRQGLHTLNQTDIPFSVEKKTVILIDDVL
                     YTGRTIRAAMDALLAFGRPAKVELCVLIDRRFSREFPIQADYAGKSIDTIITQKVKVL
                     WKERDGTEEVVLL"
     gene            3335..>4066
                     /locus_tag="VGM24_04780"
     CDS             3335..>4066
                     /locus_tag="VGM24_04780"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY4154713.1"
                     /translation="MQLSTKHLLGIKDLPASDIQTILDTATQFKEVLQRPIKKVPTLR
                     DVTIVNLFYENSTRTRISFELAEKRLSADTINFTASGSSVSKGESLLDTVNNILSMKV
                     DMVVMRHSASGAPHFLAKHIPAAIVNAGDGINEHPTQALLDAFSIREKLGSLEGKKIA
                     IIGDIMHSRVALSNIYLLKKMGAEVMVAGPPTLIPVHIREAFDIRVEYNLRKALEWCD
                     VANVLRIQLERQNQVLFSSLREYSLA"
BASE COUNT         1107 a          998 c         1071 g          890 t
ORIGIN      
        1 cgatatgagg tcccggctga gcggactgag gaggtttctt tgacgtcgcc aaccggcaaa
       61 tcaaaatcga cgaagaaaaa caactgaccg gaagccccgg gttctggaat gacaacgagc
      121 gggcaacggg cattctcaaa aacattaagg taaacgagta ctgggtaaaa ctctatgaat
      181 ccgtcgggac ggcgatagag gactttgccg tattgctcga gttctggaaa gccggagaag
      241 ccaccgagga agaaacccgg gaggcttatc agaatgccct ggataaaatg gaggaagctg
      301 aatttaagag caccctcaat cagccggaag atgagcttcc ggccgttttg cagatcaatt
      361 ccggagctgg tggaacggaa agccaggact gggcggaaat gctgatgcgt atgtaccgga
      421 tgtatggaga aaaacagggc tggtccgttt ccgaactgga ttaccaggac ggcgaaggag
      481 ccggcataaa aagcgttacc ctgcaattcg aaggaccctt tgcctatgga tttctgaaag
      541 cggaaagcgg cgtgcaccgg ctggtgcgca tttccccgtt cgacagcaat gccagacgcc
      601 atacttcctt cgcctcggtt tatgtttacc cccttattga cgacagtatt gaaatcgaag
      661 tgaatgctgc tgatgtggaa tgggcttttt tccggagcgg cggtgcagga ggacaaaatg
      721 taaataaagt ggaaaccgcc gtacgcctga cccacaagcc cagcggcatt attgtggagt
      781 gccagcaggc gcgcacgcag ggggaaaatc gtgaaaaagc catggccatg ctcaagagcc
      841 gcctctacga agaagagatc cggaaaagag aggccatcaa aaatgccagc aatgccagca
      901 agaaaaaaat tgaatgggga tcccagatca ggtcctatgt attccatcca tacaagatga
      961 tcaaggatca ccgcaccgat tttgaaaccg ggaatgtgca gccggtgatg gatggggaac
     1021 tcgacggatt tataaaagcg tacctgatga gcgaaaaaga aatggctaat taaaccggta
     1081 accactcaac gagagtagca tgaaccttgg ttattttcat tacgcgatgc cttccaatga
     1141 accggtgctg aattatgccc ccggatcagc ggagaaaatt tccctcaaaa aagcgatcag
     1201 ggaaataaaa gcacaggagc acgatattcc gatgatcatc ggaggaaagg aaatccgcac
     1261 cgggaataaa atcaaaatga gcccgccgca tgagatctcc cataccctgg gttattttca
     1321 ccgggccggc cgggagcaga ttgaacaggc tgttgaggcg gcgctgcaag ctaaacccgc
     1381 ctgggagcgg ctgaactggg aaaaccgggc ggctattttc ctgaaagccg ccgatctgct
     1441 ggcagggaag caccgggctt atatgaatgc ggccacgatg atcgggcaaa gcaagaatcc
     1501 ctttcaatct gaaattgacg gaatatgtga actcattgac tttctgaggt ttaatgtcca
     1561 ttatctcagt gaaatataca ggcagcagcc aagcagttcc cccggggttc ataaccggat
     1621 ggagtaccgg ccgctggaag gctttgtact ggccatcacg ccattcaatt ttacggcaat
     1681 cgcgggtaac ctgccctctt ctgcagccct ttgcggaaat acagtggtgt ggaaaccggc
     1741 agatacccag gtcttcagcg cccatttttt tatgaggatc ctgcaggaag ccggattgcc
     1801 ggacggggtg atcaacctgg tttatccgga agggaccgaa ctgggatccg tttgttttag
     1861 tcaccgggat tttgctggga ttcattttac cgggtccact gccgttttta ataaaatctg
     1921 gaaaaccatc ggagaaaata tcgggaacta taaatcatat ccccggatcg tgggcgaaac
     1981 ggggggaaag gatttcgtcc ttgttcatcc ggaggccgat gccgcggtcg ctgtcaccgc
     2041 actggtccgg ggtgctttcg agttccaggg acaaaaatgc tccgctgcgt caagggctta
     2101 tcttccttcc aatcttgcgg cggagatccg ggaaaaatta ttggccgcgg tcaaaacgct
     2161 gagagtcggt actgtggagg atttccggaa cttcgtgaat gccgtgatag atgaaaagag
     2221 tttcgataag atcaggggat atattgatcg cgcggagaaa gatcccaagg caaaaatcat
     2281 agccggcgga aaaacggaca aatcaaaagg gtattttatt gaccccacga tcatcgaaac
     2341 cagcgacccg gaatatatta ccctgaaaga ggaaatcttc gggcccgtgc tgactattta
     2401 cgtttacccg gagcaggaat ttgaacagac cctggatctg gttaaccgga cttctcccta
     2461 tgcccttacc gggtccatta tttcccgcaa ccggcaggcc atcgaacagg cgacggaaaa
     2521 actccgtcag gcggccggga acttttatat caacgacaaa ccgaccggag cggtcgtggg
     2581 ccagcaaccc tttggcggcg cccggggcag cggcacaaat gataaggcag gatccatcct
     2641 gaacctctac cgctggttaa gcgccagaac gataaaggaa acctttcagc cggctaccga
     2701 tcatggatat ccttttttgg gtgaagcatg aatgcatttc ctatttttat aaaaaatacc
     2761 ggttttgaag acgatcctta ctgaagaaca actgggcgta accattcagc ggctcagtca
     2821 ccagctgctg gaaaacctgc tccagtatga tgagctggtg atcatcggaa ttcagccgcg
     2881 gggaattttc ttttccgacc gcatcgtaaa gcagatcaac cagcagatcg ctccccggaa
     2941 gattacctac gggaaactgg acatcacttt ttaccgggac gatgtccgcc agggcctgca
     3001 tacccttaac caaaccgata tccctttttc cgttgaaaag aaaaccgtga tcctgatcga
     3061 cgatgtattg tataccggaa gaacgatccg ggcggccatg gatgccctgc tggcgttcgg
     3121 gcgtccggcc aaagtggaat tatgtgtttt gattgaccgc cgtttcagca gggagttccc
     3181 gatccaggcg gattacgccg gaaaatccat cgataccatc atcacccaga aagtaaaggt
     3241 tctgtggaaa gaaagagacg gaacggaaga ggtggtcctg ctatgacaaa gctgttttgg
     3301 agattggcta acgaaaatct aactttgcct tttaatgcaa ctcagtacca aacatcttct
     3361 tggcattaaa gacttacccg cttccgacat ccagactatt ttagataccg caacccagtt
     3421 taaagaagtt ctgcaaagac cgatcaagaa agttcccacc ctccgggatg taaccattgt
     3481 gaacctcttt tatgaaaact ccacccgcac ccgcatttct tttgagctgg cggaaaagcg
     3541 gctctccgca gataccatca atttcaccgc aagcggttcc tccgtttcca aaggggaatc
     3601 cctgctggat acggtcaaca atattctatc catgaaagtg gacatggtgg ttatgcggca
     3661 cagtgccagc ggagcccctc atttcctggc caaacacatt ccggccgcca tcgtgaatgc
     3721 aggcgacggc atcaatgaac atccgacgca ggctttgctc gacgcttttt cgatccggga
     3781 aaaactcgga tccctggaag gaaaaaagat agcgatcatc ggggatatca tgcattcccg
     3841 cgtcgccctg agcaatatct atctgcttaa aaaaatgggc gccgaagtga tggtggccgg
     3901 tccgcctacc ctcatcccgg ttcatatcag agaagcattt gatattcgcg tggaatacaa
     3961 tctccggaaa gcgctggaat ggtgtgatgt ggccaatgta ttgcgcattc agctggaaag
     4021 gcagaaccag gttttgtttt cctccttaag ggaatatagc ctggct
//
LOCUS       DASYCU010000235         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3532390, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000235 DASYCU010000000
VERSION     DASYCU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532390"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VGM24_04785"
     CDS             <1..917
                     /locus_tag="VGM24_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4154714.1"
                     /translation="LETGFLKDRLYLTVNWYRNISDNQIIRYSLPDQTGFTSVLRNFP
                     GVVENSGMEFSLEGTIIKNQKASWNLSINISLNRNKLLSFPGLANSSYSENYVVDKPL
                     NTFRGLHYTGIEALTGIYQFDDINKDGIIDNNDFRWIGTTDPVYFGGFSNTIRLGSFE
                     LNLLLEFRKQKGPHTVYSSGIAAGDIINQPAEVLDRWQMPGDIKKFEKFTQDFTNPAY
                     NPAIYNMASSDAALTDASFIRVKNISLSYNFPSGLIRKLKMESWRIYLQGRNIATITR
                     YKGYDPETRSGSSLPPLYQITAGMQIAF"
     gene            927..2315
                     /locus_tag="VGM24_04790"
     CDS             927..2315
                     /locus_tag="VGM24_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154715.1"
                     /translation="MKRPFINTFLASLVVFALGGNSVSCKKFVEVPPPGDQLNSELVF
                     ADSATATSAITGMYGDMMTTGNLFTNMSTTIYAGMSADELYSFTPSLKDEFFEDQITQ
                     ANHVNINNSFWLPSYKYIYTANLAIEKLSGTTLLSTALRNQLMGEALMVRAFCYFYLV
                     NLFSDVPLVTGTDYNQNGAIPRTRSGEVYQQIITDLKNAEGLLAADYPSAERVRPNKW
                     TATALLSRTYLYTGQWQEAESAADSIINSGAYSLEPDLNNVFLKGSNETIWQLEPVRP
                     GFNTFEGQSILPFNANTRPTYIFNNSVSDAFETGDLRKDAWTQTRLFAGQTLSYPYKY
                     KVYKGDDLSEYYVVLRLAEQYLIRAEARMQLQKFSAARSDINIIRNRAGLDDTGASDL
                     PSLESAIEQERKIELLAEWGHRWFDLKRTGRADPVLAPFKGSTWQSTDVLWPIPQEQI
                     NLNPLLIQNPGY"
     gene            2336..>2637
                     /locus_tag="VGM24_04795"
     CDS             2336..>2637
                     /locus_tag="VGM24_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY4154716.1"
                     /translation="MKTVFAWSLLLFLTGKQLTAQSMGKIAIGEKIPPITIKNVYQNP
                     GAQISFQQLKGKLIIIDFWNSWCGTCIEGFPDLERLQQQFSDRIGILLVTSDPERQV"
BASE COUNT          731 a          648 c          596 g          662 t
ORIGIN      
        1 cgctggaaac gggctttttg aaggaccgct tatacctgac cgtaaactgg tacagaaata
       61 taagcgacaa tcagattatc cggtatagtc tgcccgacca gaccggattt acaagcgttc
      121 tcagaaactt tcccggggtg gtggaaaaca gcggaatgga gttttccctt gagggaacga
      181 tcatcaaaaa tcagaaagcc tcctggaacc tgagtattaa catcagcctg aaccggaata
      241 agctgctgtc ttttccggga ctggcgaaca gcagctattc ggaaaactat gtggtggata
      301 agccacttaa cacgttcagg ggattacact ataccggtat tgaagcgttg accggcatat
      361 atcagtttga tgatataaac aaagacggga taattgacaa caatgatttc agatggatcg
      421 gaacgaccga tccggtatat ttcggaggat tttcaaatac gatccggctt ggatcttttg
      481 agttgaacct gttactggaa ttcaggaagc aaaagggccc ccataccgtt tatagttcag
      541 gcattgcagc aggagacatt atcaaccagc cggccgaagt gctggatcgc tggcaaatgc
      601 cgggagatat taaaaaattc gaaaaattca cccaggattt tactaacccg gcatacaatc
      661 cggctatcta taatatggca agttcagatg ctgcgcttac ggatgcctct tttatccggg
      721 taaaaaacat ttctctttcg tataattttc cttcaggact gatccggaaa ctaaaaatgg
      781 agagttggcg catctacctg cagggccgga atatagcaac gatcacgcgg tacaaaggct
      841 atgatcccga aaccagatcc ggttcttccc tgcccccctt atatcagatt acagcaggca
      901 tgcaaatcgc tttttaaaac agaaaaatga aaagaccttt tataaatacg ttccttgctt
      961 ctttggtcgt tttcgccctg ggcggaaatt cggtctcctg caaaaaattt gttgaagtgc
     1021 ctcctcccgg cgaccagctg aacagtgagc tggtatttgc ggacagcgcc actgctacct
     1081 cagccattac cggtatgtat ggcgatatga tgacgaccgg gaacctcttc accaatatgt
     1141 ctaccaccat ttatgcgggc atgtctgctg atgagctcta ttcctttacc cccagtctga
     1201 aagatgagtt ttttgaggat cagatcacgc aggcaaatca tgtcaatatc aataattcct
     1261 tctggcttcc ttcctataaa tacatttaca cagccaacct cgccatagaa aaactttccg
     1321 gaacaaccct gctctccacc gcgctaagga atcaattaat gggggaggcg ctgatggtcc
     1381 gcgcattctg ttatttttac ctggtaaatc ttttttcaga tgttccactg gtcaccggca
     1441 ccgactacaa tcaaaatggc gctattccaa gaaccagatc aggcgaagta tatcaacaga
     1501 tcattacgga tttgaaaaat gcggagggat tgcttgccgc cgattaccct tcggcggaaa
     1561 gagtgcgtcc caataaatgg accgctacgg ctcttttgtc cagaacatac ctgtataccg
     1621 gccagtggca ggaagcggaa tccgccgctg attcgattat taactcgggc gcctattccc
     1681 ttgagccaga tctcaataat gtatttctaa agggcagcaa tgaaaccatc tggcaattag
     1741 agcctgtccg ccccggcttc aatacatttg aaggacagag catacttccg tttaacgcga
     1801 acacccgccc cacgtatata ttcaataatt cagtttctga tgcttttgag accggagatc
     1861 tccggaaaga tgcctggacc caaacccgcc tatttgccgg acagaccctg tcctatccgt
     1921 ataaatataa agtatataaa ggcgacgacc tgtctgaata ttatgtcgta ctccggctgg
     1981 ctgaacaata tctcattcgg gccgaagccc gaatgcagct gcagaaattt tcggctgccc
     2041 ggtcggatat taatatcatc cgtaaccgtg ccggtctgga tgataccggc gcatccgatt
     2101 tgccttccct ggaatcagcc atcgaacagg agcgaaaaat agagctgctg gcagaatggg
     2161 gccaccggtg gtttgacctt aaaagaaccg ggcgggcaga tcctgtcctg gctcctttta
     2221 aaggaagcac atggcagtcc accgatgttt tgtggcccat tccccaggaa cagattaacc
     2281 tgaatccgtt attaatccaa aacccgggtt actaatttca acgataacca gctaaatgaa
     2341 aaccgtattc gcctggtcct tgctgctctt tttgaccggt aagcagttaa ccgcacaatc
     2401 catgggaaaa attgccatcg gtgaaaagat tcctcccatt acaataaaga acgtttatca
     2461 gaatcccggc gcccaaattt catttcagca actaaaaggg aaactgatta ttatcgattt
     2521 ttggaacagc tggtgcggga cctgcattga aggttttccg gacctggaac gcctgcagca
     2581 gcagttcagt gaccggatcg ggatcctgct ggttaccagc gacccggaaa gacaggt
//
LOCUS       DASYCU010000236         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3387032, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000236 DASYCU010000000
VERSION     DASYCU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3387032"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VGM24_04800"
     CDS             complement(<1..537)
                     /locus_tag="VGM24_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154717.1"
                     /translation="MKLKNIYLLLLLVVLTGLDTGCKKFVDVTNPDTLTDPGFWKTES
                     NVRSYNWEFYNLFAGFGNGSSTNGDFYFTAFSDDQCTSGFTQFPQNTPASNTSWTFGF
                     IRKANIMLERIDQVNMPDEAKNHWKGIAHFFRAFQYFSLVQTFGGVPWFSHSLDISDS
                     SLIYKPRDSHQLVMDSVLA"
     gene            complement(566..>2281)
                     /locus_tag="VGM24_04805"
     CDS             complement(566..>2281)
                     /locus_tag="VGM24_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154718.1"
                     /translation="LTNIATASQDRVSYNSGRLMVNTFNTYATYPNSFRGVHNVKVIA
                     GVNAENDENLGFFSTRQGLLDPTQGELGLAYGTQTNGVSNINTPDGWSTNGHGKQAFA
                     GYFGRINYDYKGKYLLELNGRYDGSSSFSPLDRWAFFPSGSIGYRISQENFMDFSKKV
                     ISDLKIRASYGTLGNQAVGGNYYLRTMNGAQASWLDNSGVYAQYITQPAAVANSLKWE
                     KVSSTDIGLDARLFDNHIGVSFDWYERNTDGMIQPTSVPSTFGTTGPRINAGNFRNRG
                     YEIMLDANYNLGKDAMIYATLGFSDGKTVFTKWSNPNESIMTAFNYVGKTYGEIWGFE
                     TAGFFADAADVTKSPSQAALQAGNFIYGPGDIKFKDLNGDGKIDGGKMTLGDHGDLKV
                     IGNTQPRYQYNARIGGSWKNFDIDVFIQGVGSRQYWAPGNIAIPLYQSLDIVYKNQLD
                     YWLPNHTNAKYPSPYPNNQTGTIGGLLNGGNNFYPQSKYLLNLAYCRLKNLTVGYTLP
                     GNLLARYSIQKFRIYLSAEDLGEISNVGAPIDPELTDLGGLNSVGRTWPFMRSYSFGV
                     QLSFQ"
BASE COUNT          563 a          557 c          575 g          586 t
ORIGIN      
        1 cgccagcacg ctgtccatga ccagctgatg agaatccctg ggcttatata tcagtgagct
       61 gtccgaaata tcgagggaat gactgaacca gggcactccg ccaaaagttt gcaccagaga
      121 gaaatactga aaggcacgga aaaaatgggc aatgcccttc caatggttct ttgcttcatc
      181 cggcatattc acctggtcga tccgttccag catgatattg gctttccgga taaatccgaa
      241 agtccaggac gtattggaag ccggcgtatt ttgcgggaac tgggtaaacc cggaggtgca
      301 ctggtcatcg gaaaaagcgg taaaataaaa atctccgttt gtactgctgc cgtttccaaa
      361 gccggcaaat aaattataga attcccagtt gtaggagcgc acattacttt ccgttttcca
      421 gaagccggga tcggtgaggg tatccgggtt ggtaacgtcc acgaatttct tacatcccgt
      481 atccaggccc gtcaggacca ccaggaggag caggagatat atatttttca atttcatgac
      541 tgaaaattaa gattgagtaa atgggttatt gaaaagaaag ctgaacgcca aatgaatatg
      601 atcgcataaa aggccaggtt ctgccaacgg aattgagccc tcccagatcg gtgagttccg
      661 gatcgatcgg agcacccaca ttggagattt cgcccaggtc ctcggcggat aaatagatcc
      721 tgaatttctg tatgctgtat ctggccagca ggtttccggg taaagtatat ccaaccgtca
      781 ggtttttcaa ccggcaatag gccaggttga gtaaatattt ggattgcgga taaaaattgt
      841 tccctccatt caacaaaccg ccgatggtac cggtctggtt attcggatat ggggagggat
      901 actttgcatt ggtatgattg ggcaaccagt aatccaattg attcttgtaa acaatatcga
      961 ggctctggta caggggaatc gctatatttc cgggcgccca gtactggcgt gaaccgactc
     1021 cctggataaa gacatcgata tcgaaattct tccagcttcc gccgatgcgg gcgttgtact
     1081 gatacctggg ttgtgtgttg ccaattactt tcaggtcccc atgatcgccc agcgtcattt
     1141 ttccgccgtc aatcttaccg tcgccgttca ggtccttgaa cttgatgtct ccgggtccat
     1201 agataaaatt accagcctga agggctgcct ggctggggga cttggttacg tccgcggcat
     1261 cggcaaaaaa ccctgcggtt tcaaaacccc agatctctcc atacgttttt ccaacataat
     1321 taaaggcagt cataatggat tcgttgggat tactccattt ggtaaatacg gttttgccgt
     1381 cagaaaagcc cagggtggca tagatcatag catccttgcc caggttataa ttggcatcca
     1441 gcatgatctc atatccccgg ttccggaagt ttccggcgtt gatccggggt ccggtggttc
     1501 caaaggttga aggaacgctg gtgggctgta tcatgccatc cgtattgcgt tcgtaccagt
     1561 cgaagctgac cccgatatgg ttgtcaaata aacgcgcatc cagaccgata tccgtggaag
     1621 atactttttc ccatttcagc gaattggcga ccgcggcagg ctgggtaata tactgcgcat
     1681 atacgccgct gttatcaagc cagctggcct gtgcgccgtt catggtccgc aggtagtagt
     1741 tccctcccac tgcctggttg cccagagtac cgtaagatgc ccggatcttc agatccgaaa
     1801 ttactttctt gctgaaatcc atgaaattct cctggctgat ccggtatccg atagaaccgg
     1861 aagggaaaaa tgcccagcgg tcaagcggag agaaagaaga ggatccgtca tagcgtccgt
     1921 tcagttccaa cagatatttt cctttgtaat cgtagttgat ccttccgaaa taaccggcaa
     1981 acgcctgttt tccatgaccg ttcgtgctcc atccgtccgg cgtgttaata ttggaaacgc
     2041 cattggtttg ggtgccgtaa gccagaccca gttctccctg ggtggggtcc agcaatccct
     2101 ggcgggtgga gaaaaagccg agattctcat cattttctgc attcaccccg gcgatcactt
     2161 ttacattgtg cacgcccctg aaagaattcg ggtaagtggc gtaggtgttg aaggtattca
     2221 ccatcagcct tccggaatta tacgagaccc ggtcctgcga tgccgtagcg atattggtca
     2281 g
//
LOCUS       DASYCU010000237         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4546500, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000237 DASYCU010000000
VERSION     DASYCU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4546500"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2088
                     /locus_tag="VGM24_04810"
     CDS             <1..>2088
                     /locus_tag="VGM24_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154719.1"
                     /translation="REISAVFNGFPNIVYVAGHEHGLQFIKSDEVQVVSGAGAKHTEA
                     KKGKYSLFADAIQGYVTVDLLGGNMLSFNYMGYENGEIKKLFAFRQPYTPTTENKFFG
                     QPISEDSIALRVHPVYDSVSRLHRFFFGENYRKEWAAQVTLPVLRISRINGGLKPLQL
                     GGGMQSKSLRLEDPEKNEWVIRSVEKATEKLLPEALRETFARDVLDDNTSAQHPFSAL
                     VVPPIAEAVRVPHANPVIGVLSSDTSLGLYNRVFSNMVVLLEEREPLGKSDNSEKMKR
                     NLLKDNENRFNGKEMLRARMLDMYLGDWDRHEDQWRWYDEDKGKDIRYLAVPRDRDQV
                     FHVDEGLIPVLASKDYVLPTIRNFDAELSHFKWLLFKTRFVNAFPEMQFSRPEWMKEA
                     EGFKQAITDSVLETALRRLPLTVYNMDHDLLLQKLRARREKIPAAMDQYYHFIQKVAD
                     IRLSNKNEWVQVDSDEKGGLQVRISRVSKTGEVRKELMNKTFDPLLTKELRIFLGNGN
                     DSVVVNSKDSRIKLRIIGGSDPKLYHFGPSGGKINLYDKDNGSVFAGDLSRLKKHISN
                     DSANTAFDPVNLYNIWMPLLTGGLNIDDGLILGAGFRFTKQEGFRKFPYASRQQLMVA
                     HSFSTSAYRISYQGEWTKLFGNADLTAHAVIRAPDNTMNFFGRGNETVYDETGNYKRY
                     YRTRFAVYQADPAL"
BASE COUNT          587 a          457 c          555 g          489 t
ORIGIN      
        1 cgggaaatat ccgcggtctt caacgggttt cccaacatcg tgtatgtggc aggacatgaa
       61 cacggacttc agtttatcaa aagcgatgag gttcaggtag tgagtggggc cggggccaaa
      121 catacggaag caaaaaaagg aaaatattcc ctgtttgcag atgccatcca gggatatgta
      181 accgtagacc ttctcggagg aaacatgctt tcctttaatt atatgggtta tgaaaacgga
      241 gagataaaaa aactttttgc tttccgccag ccttacacgc caacaactga aaacaaattt
      301 tttggccagc cgatttcaga agacagcatc gcgctgaggg tgcatccggt ttacgacagc
      361 gtgagcagac ttcaccgttt cttctttggc gaaaactacc ggaaggaatg ggctgcgcag
      421 gtaaccttac cggttctccg gatatcccgg atcaacggcg gcctcaaacc gctccagctg
      481 ggtggcggca tgcaatcaaa gtctttgcgc ctcgaagatc cggagaaaaa tgaatgggtg
      541 atacgcagtg tggaaaaggc aacggaaaaa ttattgccgg aagccttacg ggaaacattt
      601 gcccgggacg tgctcgacga caataccagc gcacaacatc cgttctccgc cctggttgtg
      661 ccgccaatag ccgaagcggt acgggttccg catgccaatc cggtgatcgg cgtgctctct
      721 tcagatacca gcctggggct gtataaccgg gttttctcga atatggttgt gttgctggaa
      781 gaacgcgaac ccctgggtaa atcggataat tccgaaaaaa tgaaaaggaa tcttctgaag
      841 gacaacgaaa acaggttcaa cggaaaggag atgctgcgcg cgcgtatgct ggatatgtac
      901 ctgggtgact gggacaggca cgaagaccag tggagatggt atgacgaaga taaagggaag
      961 gacatccggt atcttgccgt tccccgagac cgggaccagg tgttccatgt ggatgaagga
     1021 ctgattcctg ttcttgcatc caaagattat gttcttccca ccataagaaa ttttgacgcg
     1081 gagctgagcc atttcaaatg gctactattt aaaacaaggt ttgtgaatgc ctttcctgaa
     1141 atgcagtttt ctcgtccgga atggatgaag gaagcggaag gattcaaaca ggctataacg
     1201 gattccgtat tggaaacagc attgcgaaga ttgccgctga cggtttacaa catggaccac
     1261 gacctgttgc ttcaaaaact cagggccaga agggaaaaaa ttcccgcagc catggatcaa
     1321 tattatcatt ttatccagaa agtggccgat atccggctca gcaataaaaa cgaatgggtt
     1381 caggttgatt cagatgagaa gggaggtctg caggttcgga tttcaagggt cagcaagacg
     1441 ggggaggtca ggaaggaact aatgaataaa accttcgatc ccctgctgac aaaagaattg
     1501 cgcatattcc ttggaaatgg aaatgacagt gtggttgtca acagtaaaga ttcccggatt
     1561 aaactccgca ttatcggggg gagcgatccg aaattatatc atttcgggcc atcgggagga
     1621 aaaattaatt tgtacgacaa ggacaatggt tctgtattcg caggagatct ttcccggtta
     1681 aaaaaacaca tcagcaatga cagtgccaat actgccttcg acccggtaaa cctttacaat
     1741 atatggatgc cgctgctcac gggaggcctc aatattgacg atggacttat cctgggtgcg
     1801 gggttccggt ttacaaaaca ggagggtttc cgcaaatttc cctatgcatc gcgccagcag
     1861 ttgatggttg cgcattcttt ttccaccagt gcctaccgga tcagttatca gggagaatgg
     1921 acaaagcttt ttgggaacgc agatctgacg gctcatgccg tgatcagggc gccggacaat
     1981 acgatgaatt tttttggaag aggaaatgaa accgtctatg atgagacggg taactataag
     2041 aggtattaca ggacccggtt tgccgtttat caggctgatc ccgctttg
//
LOCUS       DASYCU010000238         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1501898, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000238 DASYCU010000000
VERSION     DASYCU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501898"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VGM24_04815"
     CDS             <1..315
                     /locus_tag="VGM24_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154720.1"
                     /translation="KYLNKFKNSQQQTGDSVRADKMARASLILGIVAVILVFIPWYTL
                     LFVIPLGILAIVYGKQAIKNGTTKETMAKVGKGLGLGALIGFAVEVLIATIIIVSWAS
                     WL"
     gene            complement(330..1805)
                     /locus_tag="VGM24_04820"
     CDS             complement(330..1805)
                     /locus_tag="VGM24_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY4154721.1"
                     /translation="MGKLTGFLEYKRELPKKRPVQERIKDYKEFVNPFEPVQLNQQAA
                     RCMNCGIPFCSNGCPLGNVIPEFNDAVYRNSWLEAYEILSSTNNFPEFTGRICPAPCE
                     SSCVLGINQPAITIEEIEKHIIEIAFEKGFVKAKEPNVRTGKKIAIVGSGPAGMAAAA
                     QLNSAGHSVTVFERDDAAGGLLRYGIPDFKLEKWVIDRRIGILEQEGIVFRCNANVGV
                     NVHINDLMREFHAIVLTGGSTIPRDLPIPGRELKGVHFAMDFLKQQNKRISATEISAE
                     NILAKNKNVIVIGGGDTGSDCVGTSNRQGAKSITQFELLPEPPKERTAFMPWPTYPMI
                     LKTSSSHEEGCERHWAMGTKEFVGDEKGNLTALKIVDLEWKLAEDGRPARFTEVPGSE
                     RVMPCELAFLAMGFVHPQHEGMIQDLGVELDERGNVKATEKSFQTNIAKIFSAGDMRR
                     GQSLVVWAISEGRECARKVDEYLMGSSMLETKDHSLIGVTY"
     gene            complement(1809..>2596)
                     /locus_tag="VGM24_04825"
     CDS             complement(1809..>2596)
                     /locus_tag="VGM24_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEY4154722.1"
                     /translation="AIEAGQKITASFNVKNTDRTIGTLLSNEISKIFKSKGLPQDSIH
                     FKLKGTAGQSFGAFNTQGVTLELEGDANDYFGKGLSGARLIVYPAADAGFVPEENFII
                     GNVAFYGATSGEAFIRGRAGERFAVRNSGANAVVEGIGDHGCEYMTGGRVVILGSTGR
                     NFAAGMSGGIAYVYDVGGKFASLCNPDMVDLDPLDEEDALEMKEMIARHYACTASKVA
                     RFVLDDFENQLRNFVKVFPKDYKKVLLEKKSPVRANSQTNKRN"
BASE COUNT          596 a          683 c          577 g          740 t
ORIGIN      
        1 aagtatttaa ataaattcaa aaacagtcag cagcaaacag gcgattccgt ccgggcggat
       61 aaaatggcca gggcttcctt aatccttggc atcgtcgcag tcattttagt atttattccc
      121 tggtacacgc ttttgtttgt aataccgctg ggaattctgg ccatcgttta tggaaagcag
      181 gcaataaaaa acggaacgac gaaggagaca atggccaaag ttggaaaagg cctgggcctt
      241 ggcgcgctta ttggtttcgc ggtcgaagtt cttatcgcaa ccattataat cgtaagctgg
      301 gccagctggc tataaatatc aggtgatatt tagtatgtca ctccgatcag tgaatgatct
      361 tttgtttcca gcatggatga gcccatcagg tattcatcca cctttctggc gcattccctt
      421 ccttcactga tggcccagac aaccagggat tggccgcggc gcatgtcgcc ggcactgaat
      481 atcttggcga tattggtttg gaaagacttt tccgttgcct tcacatttcc ccgttcatcc
      541 agttccacgc ccagatcctg aatcattcct tcatgctggg gatgcacaaa tcccatggcc
      601 agaaaggcca gctcacaggg cataaccctt tccgatcccg gaacttcagt aaaccgcgcc
      661 ggccttccat cctctgccag cttccattcc aggtccacaa tcttcagtgc ggtcaggttg
      721 cctttttcat caccaacgaa ctctttggtg cccatcgccc aatgtctttc acaaccttct
      781 tcatgggaag aagaagtttt tagtatcatc gggtaagtag gccagggcat aaaagccgtt
      841 ctttcttttg gtggctcggg gagcaattca aattgtgtga tggatttcgc gccctgccgg
      901 ttggaagtgc caacacagtc gctcccggtg tcgccaccac cgatcacaat tacattttta
      961 ttttttgcaa gaatattttc tgcgctgatt tcggttgcgc ttatccgttt gttttgctgt
     1021 tttagaaagt ccattgcaaa atgcacacct ttcaattccc tgccgggtat gggtaagtcg
     1081 cggggtatcg tggacccgcc cgtcagcaca atggcatgga attccctcat caggtcattg
     1141 atgtgcacat tcacgcccac gttggcattg caacggaata caatcccttc ctgctccagg
     1201 atgccaattc ttctgtcgat cacccatttt tccagtttga aatccggaat cccgtaacgg
     1261 agcagtccgc ctgctgcgtc atccctttca aaaacagtca cggaatgccc ggcactgttg
     1321 agctgggcag ccgctgccat tcctgcgggg cctgaaccca caatggctat tttctttccg
     1381 gtccttacat tcggttcctt tgcctttaca aagcccttct cgaaggcaat ctcgatgatg
     1441 tgcttttcga tttcttcaat ggtaatggcc ggctggttga tgcccagtac gcagctgctt
     1501 tcgcaggggg cgggacagat tcttccggtg aattcgggaa aattattggt ggaagaaagg
     1561 atctcatacg cttcgagcca cgaattgcgg taaacggcat cattgaattc cggaattaca
     1621 tttccgagcg gacaaccatt gctgcaaaat ggaattccgc agttcatgca gcgcgcggcc
     1681 tgctggttta actgtaccgg ttcaaacgga ttgacaaact ctttataatc ttttattctt
     1741 tcctgaaccg gacgcttttt agggagctcg cgcttatatt ccaggaaacc tgttagtttt
     1801 cccatttttt agtttctctt attggtttgt gaattggccc ttacagggga tttcttttcc
     1861 agcagtactt tcttataatc cttcggaaat acttttacga aattccggag ctggttttca
     1921 aaatcatcca gcacgaaacg ggcaacctta ctggctgtac aggcataatg tctggcaatc
     1981 atttccttca tctcgagggc atcttcctca tcgagcggat cgaggtcaac catatccgga
     2041 ttacataaag aggcaaactt gccgcccaca tcatagacat atgcgattcc gccactcatg
     2101 cccgccgcaa aattgcgccc ggtagatccc aggataacca ccctgcctcc ggtcatgtat
     2161 tcacatccat gatccccgat tccttccacc acggcattgg cgcctgagtt cctgacggca
     2221 aagcgttctc cggctcttcc cctgataaag gcttcgccgg aagtagctcc gtagaaggcc
     2281 acgtttccga tgatgaaatt ttcctccgga acaaatccgg catctgctgc agggtataca
     2341 atcagtcttg caccggacaa tccctttccg aaataatcat tggcatcccc ctccagttcc
     2401 agcgtcacac cctgtgtatt aaatgcgcca aaactttgcc cggccgttcc tttgagttta
     2461 aaatgaatgc tatcctgtgg taatcctttt gatttaaata ttttcgatat ttcattggaa
     2521 agaagcgtgc cgatggtgcg atcggtattt tttacgttga aagaagcggt tattttctgt
     2581 ccggcttcaa ttgccg
//
LOCUS       DASYCU010000239         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4546827, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000239 DASYCU010000000
VERSION     DASYCU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4546827"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VGM24_04830"
     CDS             <1..1182
                     /locus_tag="VGM24_04830"
                     /inference="COORDINATES: protein motif:HMM:NF039201.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4154723.1"
                     /translation="NVSNNLSLTSGLLNTTASYMLTMLNGSTTAVGNVLSTSYVNGPM
                     RYKKSSAGSSILNFPIGNNIDCRPVALTVNHANGNAYTYQAQVFNASAAALGYSLPVT
                     VDVVSSVHYYTISRVNAASVNQPSLDLSGNQTIQVFFGSNDVVSNGGMLTVVKNTNAA
                     PTSWIDIGGTGGPVFTGANLSGSVTSTSAPSAFNSFSTFALGNKLGGNNILPIELLYF
                     NARPENGRTALSWATATESNNSYFTIEKSRDGSAFEFLQKLDSKAPNGNSSTPLNYTA
                     NDMNPFNGMNYYRLKQTDLDGNFKYSAIVQVSFDNRKTISVYPNPTSGILYISGVSIN
                     QSSLRVEWFDMSGRSLLQQTVPVQNGMATLNASFNSGVYILKIISADGSIKLQNVIIT
                     K"
     gene            complement(1172..1318)
                     /locus_tag="VGM24_04835"
     CDS             complement(1172..1318)
                     /locus_tag="VGM24_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154724.1"
                     /translation="MGRPRAAVKIYSKIVLIGKIWRKNLDLCKNPVTGKDPGDRINPD
                     LITS"
     gene            1389..1823
                     /locus_tag="VGM24_04840"
     CDS             1389..1823
                     /locus_tag="VGM24_04840"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4154725.1"
                     /translation="MASLAGIAGIGMSAKAMKPSKTEKAEKITNNITYFQEVPLFSGS
                     TRMGDIVYIAGKGAHFEGDIKSHTDHVLKELEEELVKAGSSMEKVLKVNVYLNDIADY
                     QGMNEVYKGRFGKNPPVRTTVAVMKGGVPGNSLVEIDCIAHV"
     gene            1880..3094
                     /locus_tag="VGM24_04845"
     CDS             1880..3094
                     /locus_tag="VGM24_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY4154726.1"
                     /translation="MKRRDMLKGISILPFAATLGGDLLVPEKAKAGKLPKSKRNLFEE
                     LGVRPFINAAGTYTFMTGSLMREEVIDAIRNTSEDFCLLDELQDKVGEKIATMVHAES
                     AVVTCGAFSGLTLGVAGVLTGNDVEKVKMLPQLEGTGMKTEILLQKAHGIDYNHALTN
                     TGAKLITIETAEELEKAINSNTALMHFLNIEADHGQIMHEEWVAIGKKHGIPTSIDIA
                     ADVPPVSNLWKFNDMGFHFVVISGGKAIRGPQSAGLLMGKKDIITAARLHMPPRGFNI
                     GRGMKVNKEEIFGMYAALQHYINYDHEAEWKIWEANIATIENAIKPVKGVSTNVFVPK
                     LGNITPTLDLTWDNSRLSIKDLVDKLRNGDPSIEVMPHEKGIHITVWVMRPGQEKLVA
                     KRLREVFSAALA"
     gene            3158..>4821
                     /locus_tag="VGM24_04850"
     CDS             3158..>4821
                     /locus_tag="VGM24_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4154727.1"
                     /translation="MNIRPDPFPEKFKINKTIRTVSLFALAVFFFFALSRCKSSKKEP
                     DDLSREWIAPPETQKLRPAFGDFDLAQKKGMELYALQCVSCHGVQGYGDGGAGKTAEQ
                     KPANFHSDKVKKESIGALFWKINTGRGVMPSFKTAFTDEQRWELAAYLKGFSFRQEVV
                     VPPRALRPDIKIEHLMNIGPVAVRLLQNPVTGDLYYTTFDGDVFQIRNLNSGHPDSVK
                     ILTAADHHIGRLQGAIWNRNTLFLCGNVDLDNKKGTSGRMVRFEFGKSGPPQMSVVFT
                     TPEYGANKTIYDHGWNALAVSPDGKYIFVNSGARTDHGEIQDDGGLYPNARDNAFTSK
                     IFRFPIDTKDLVLPPDTAKLRAEGYLYAEGLRNAFDMAFDGQGNLFAVVNSSDYDCPE
                     DMFWLRQNHHYGFPWVMGGIENPQQFPGWKPDPATDSFINKASHSWRVHYYYDDSSFP
                     KIPPGVKFSAGVQNMGPDANEYRGHSGRVLDGDQTGVAVSTFTPHSSPLGLFFDTKNA
                     LSDDFRGDGFVIRYGLGRNEAMMRAFTTEGSDLLHLQMTYDKEMDN"
BASE COUNT         1295 a         1210 c         1202 g         1114 t
ORIGIN      
        1 aatgtgtcca ataatctttc gctgacctcc ggcctgctga ataccacagc atcctatatg
       61 ttaaccatgc tcaatgggtc aaccactgcc gtcggcaatg ttctttcaac cagctatgtt
      121 aacggcccca tgagatacaa aaaatccagc gccggttcca gcatcctgaa tttcccgata
      181 ggaaataata tagactgccg tcccgtcgcg ctcaccgtaa atcatgccaa cgggaatgcc
      241 tatacctacc aggcgcaggt attcaatgca agcgccgccg ccctgggata cagcctgccg
      301 gttaccgtgg atgtggtttc gtccgtgcat tattatacca tcagccgggt aaatgccgct
      361 tccgttaacc aaccgtccct ggacctgagc ggcaatcaaa caatccaggt gttcttcggc
      421 tcaaacgacg tggtgtcgaa tggcggcatg ctgaccgtcg ttaaaaacac caatgccgct
      481 ccgaccagct ggatagacat cggcggaacg ggcggcccgg tatttaccgg cgcaaatctg
      541 tcgggaagcg ttacttcaac ctctgcgccc tcggccttca attctttttc cacctttgct
      601 ttaggcaata agctgggcgg aaataatatc ttacccatcg aactgcttta ttttaacgcc
      661 cggccggaaa atggcaggac agcgcttagc tgggcgaccg caacagaatc aaataacagc
      721 tattttacaa tagaaaaaag ccgggatggg tctgcattcg agttcctgca gaagctggat
      781 tcaaaggcgc ccaacgggaa cagcagcacc cctttaaatt atactgcaaa tgatatgaat
      841 ccgtttaacg gaatgaatta ctaccggctg aaacaaaccg atctggatgg caactttaaa
      901 tattccgcca tcgtgcaggt aagtttcgat aaccggaaaa ccatatcggt ttatcccaat
      961 ccgacctcgg gcatccttta tatcagcgga gtcagtatca atcagtccag cctgagggtg
     1021 gaatggttcg atatgagcgg aaggtcctta ttgcagcaga ccgttcctgt ccaaaacgga
     1081 atggccacgc tgaatgcaag ctttaacagc ggcgtttata tattaaaaat catttctgca
     1141 gacggtagta taaaactgca aaatgtgatc atcacgaagt gatcagatcc ggattgatcc
     1201 ggtcgccggg gtcctttccg gtgaccggat ttttacacag atccagattt ttgcgccata
     1261 ttttcccaat aagcactatt ttactataga ttttgaccgc cgcccggggt cttcccattc
     1321 atttttttat aacctcaaat aaacttcaca ggatgaaaac acaacgccgg tcagctttaa
     1381 aaaagattat ggcttctctt gcaggcattg ccggaatcgg gatgtcggcc aaggccatga
     1441 agccatccaa aacggaaaaa gcggaaaaaa taacgaacaa tatcacctat ttccaggagg
     1501 tacctctttt ttcaggatcc acccgcatgg gagatatcgt ctacatcgcc ggaaagggcg
     1561 cccattttga aggagacata aaatctcata cggatcatgt gctgaaagaa ctggaggagg
     1621 aactggtgaa agcgggttcg tcgatggaaa aagtactgaa ggtaaatgta tacctgaatg
     1681 atattgcgga ttaccaggga atgaatgagg tttataaagg aaggtttgga aaaaacccac
     1741 cggttcggac aaccgtagcc gttatgaaag gaggagtacc cggaaattcg ctggttgaaa
     1801 tagactgtat cgcgcacgtt taatttccgg ccccttccat ctttctaacc aattttccta
     1861 accaaaacaa ttgagccata tgaaacgcag agacatgcta aagggcattt ccattttgcc
     1921 atttgccgca acccttggcg gcgatctcct ggtgccggaa aaagctaagg ccggcaaact
     1981 tccgaaaagc aagcgaaacc tgttcgagga gctcggcgtc cggccattta tcaatgcggc
     2041 cggcacctat acatttatga cgggctcgct catgcgggaa gaagtgatcg atgccatccg
     2101 gaacacttcc gaagattttt gcctgctgga tgaactgcag gataaggtgg gtgaaaagat
     2161 cgcaaccatg gttcacgcgg agtctgcggt tgttacctgc ggggcttttt ccggcctcac
     2221 cctgggcgtg gcgggcgtat tgaccggcaa tgacgtagag aaggttaaaa tgcttccgca
     2281 gctggaaggc acgggtatga agaccgaaat actgctccag aaagcgcatg gcattgatta
     2341 caaccatgcg ctcacgaaca ccggcgccaa actgatcacg attgagacgg cggaagaact
     2401 ggaaaaagcc atcaacagca ataccgccct catgcatttc ctgaatattg aagcggacca
     2461 tggccagatc atgcatgagg aatgggttgc cattggcaaa aagcacggca taccaacgtc
     2521 tattgatatt gctgcagacg ttccgccggt ttcaaacctc tggaagttca acgatatggg
     2581 gtttcatttc gtggtcatct ccggtggcaa agccatccgg ggtccacaga gtgccggctt
     2641 gctaatggga aaaaaggaca tcatcacggc cgccaggttg catatgccac cgcgcggttt
     2701 caatatcggc aggggaatga aggtgaacaa agaagagatc tttggaatgt acgcggcatt
     2761 gcaacactat atcaactacg atcacgaggc ggaatggaag atctgggaag ccaatatcgc
     2821 cacgatagaa aatgccatca aaccggtgaa gggagtcagc accaacgtct tcgtgcccaa
     2881 acttggcaat atcacgccca ccctggattt gacctgggac aacagccggc tctccataaa
     2941 ggatttagtg gataaattaa ggaatggtga cccatcgatc gaggtgatgc cacacgagaa
     3001 gggcattcac attaccgttt gggtgatgcg gcccggccag gaaaagctcg tggcaaaaag
     3061 attaagggaa gtattttccg ctgccctggc ctgatgggtt tcatcccctc cctgtaaaat
     3121 gtcctttcat ttttggttta aaaccattcg ataaataatg aacataagac ccgacccctt
     3181 ccctgaaaag tttaaaataa acaaaacgat ccggacggta tccctctttg cgcttgcggt
     3241 ttttttcttt ttcgctcttt cccgctgcaa atcatccaaa aaagaacccg acgacctgag
     3301 cagggaatgg atcgctccgc cggagacaca aaagttaaga ccggctttcg gagacttcga
     3361 tctggcacaa aaaaaaggaa tggagctata cgcccttcaa tgtgtatcct gccacggagt
     3421 gcagggttac ggggacggag gagcgggaaa gactgcggag cagaagccgg ccaatttcca
     3481 ctcggacaag gtaaagaaag agtccatcgg cgccctgttc tggaaaatca atacgggcag
     3541 gggagtcatg ccttccttca aaaccgcctt tacggatgaa caacgctggg agctggctgc
     3601 ctatttaaag ggattttcct tccggcagga agttgttgtt ccgccaagag ccctgcgtcc
     3661 ggatatcaaa atcgaacatt tgatgaatat cggaccggtg gcagttcgtc tcctgcagaa
     3721 cccggtgacc ggagacctat attatacgac tttcgatggc gatgtttttc agatcagaaa
     3781 tctcaacagc ggtcatcccg attccgtgaa gatcctgacc gcagcagatc atcatatcgg
     3841 aagattgcag ggcgccatct ggaacaggaa tacgcttttc ctttgcggca atgtggacct
     3901 ggacaataaa aaaggaacga gcggaagaat ggttcgtttt gaattcggca aatcggggcc
     3961 gccccagatg tcggtcgttt tcaccacgcc cgaatatgga gcaaataaaa ccatttatga
     4021 ccatggctgg aatgcgctgg cggtaagtcc ggacggcaaa tacatttttg tgaacagcgg
     4081 cgccaggacc gatcacggtg aaatccagga cgatggcggt ctttatccca atgcccggga
     4141 caatgccttc acctccaaaa ttttcaggtt tcccattgat acaaaggacc tggtattgcc
     4201 cccggatacg gcaaaactcc gggcggaagg atatctctac gcggagggcc ttcgcaatgc
     4261 ctttgatatg gcttttgacg gccagggtaa cctttttgcg gtcgtcaact cttccgatta
     4321 tgattgcccg gaagatatgt tctggttgag gcagaatcat cactacgggt ttccctgggt
     4381 gatgggcgga attgaaaatc cccagcagtt cccgggatgg aaaccggatc cggccacgga
     4441 ctccttcatc aacaaggcct ctcattcctg gcgggtacat tattactatg atgattccag
     4501 tttcccgaaa attcccccgg gagtgaaatt ctccgccggc gttcagaata tgggtcccga
     4561 tgcaaacgaa taccggggcc attccggccg ggtcctcgac ggagatcaga ccggcgtcgc
     4621 cgttagcacc ttcactcccc attcatcgcc actgggcctg tttttcgata ctaaaaatgc
     4681 gctttcggac gatttccgcg gagacgggtt cgtgatccgt tatggcctgg gcaggaacga
     4741 agccatgatg cgcgctttca ccaccgaggg atcggacctc ctgcatcttc aaatgaccta
     4801 tgataaagag atggataatt t
//
LOCUS       DASYCU010000240         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3821165, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000240 DASYCU010000000
VERSION     DASYCU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3821165"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VGM24_04855"
     CDS             complement(<1..587)
                     /locus_tag="VGM24_04855"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154728.1"
                     /translation="MRFKPNKTVILVVILVISGSLLPSCTKYLSKKPDSSVAVPTTLN
                     DLQSLLDYSSRMNLQTTSNFGEASADDYFLPEEAYNELDVYGKKIYTWTPADYSYPND
                     WSGAYEPIYTSNYCLDQIQKIQKTEMNAVEWNNVYGSSLFYRSYYFLDLLWAFAKAYD
                     SSTFETDLGIVLRTGSDFSVPSVRSNVKDCYMQVLT"
     gene            complement(716..3829)
                     /locus_tag="VGM24_04860"
     CDS             complement(716..3829)
                     /locus_tag="VGM24_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154729.1"
                     /translation="MKKIFWIIGNMILLTIGSFAQIPQSEGLVSGTVISSKDGQTLAG
                     ATLSFTRNHFSLTCDASGKFSASLSHLPDTLFVSHIGYHSKFIMIRKPALELTISLDA
                     TGSSLDSVVVVNTGYQSVKSNEMNGSVVLIDNKTLNQQAGTDILNRLQNVTSGLAFNP
                     GYGNGNFENKTNISIRGLSTINGPLDPLIVLDNFIYEGNLANINPNDIESITVLKDAA
                     AASIWGSRAGNGVIVITTKKGRFNQGTKIDFSSGVIITEKPDLFRLPEMSVSDEIGME
                     QFLFSKGYFNSAINRKYTALPPAVEVFLKKRNGLISSADSASMVNALEGIDSKQQYMD
                     HFYQNAVVQQHAISLRGGTDKLAWLASGAFDRTVNNQGALFQKINIRFNNSYKAGRNI
                     QLDWGVYYTNSKSELGRPDYATATRINGRHIMYANLVNPDGSPAAVPQQFRSAYTDTA
                     GGGKLLDWKFYPLDEYKHARSTSRVEDIVANIGLDLKIIDPLKFNIQYQYSTQRGGTE
                     NLSDLQSYDTRNTINLFSQVDRSTGIVSYIVPLGDVLRLSENVQRSQNLRGQFNFNKS
                     LANQHITAMAGAEIREVVDNADNSIYYGYHSDPLSFANVDFVNYYPTITGGLQTISGP
                     SMSSHTENRFISLFGNLSYKYKEKYILYASARKDGSNILGVKANDQWKPLWSAGASWE
                     PSKENFYHLSWLPILKLRITYGYSGNVDLGNSALPLAQFGSDYITSLPNAVIISPSNP
                     GLRWEQTGQLNLGFDFNTKNGRLKGSVDYYSKRGKDLYGPTPFDYTTWGMSNIITKNV
                     ASMKGNGMDVILNARVIDKGFKWESQILFNYNVSKTDKYFASTSGSIYTFRGAGRKIA
                     PVNGKPLYAIAVYKWGGLDSLGNPRGYLKGQLSTDYSGISNEAFKGGLANGTLYYKGS
                     AIPVYFGSWINTFSFRQLSVSVNISYKLGYYFLRPALSYGSTVSGLGGNKEYADRWQK
                     PGDEKHTQVPSFIYPANSSRDGFYQDAEINALKGDHFRLQYINLEYSLPKMTKWFPLI
                     FSRSIST"
     gene            complement(3848..4423)
                     /locus_tag="VGM24_04865"
     CDS             complement(3848..4423)
                     /locus_tag="VGM24_04865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEY4154730.1"
                     /translation="MRSLKNCLLAILWPVVNGYAQSPPSQAKPLNIGDPVPDILISNL
                     HNYPVSKIHLSDLKNKLVILDFWAIWCGSCLSYFPKIDSLQTRFKEKLLFILIEDSTS
                     TVDSKEKVDSFLQLKRNEFLWASRLVFATERLKYLYGLFPHMFLPHYIWIGRKHKILA
                     ITSSEEITEINIRAVLNGEKIHLPFKADRYK"
BASE COUNT         1206 a         1016 c         1093 g         1322 t
ORIGIN      
        1 gtaagaacct gcatatagca atcctttaca ttcgaccgca cggatgggac actaaaatca
       61 gatccggttc tgagtacaat accgagatcc gtttcaaaag tggaagaatc gtatgctttt
      121 gcaaatgccc aaagcaggtc caggaaataa tagcttctgt aaaacagcga ggacccatac
      181 acattattcc attctactgc attcatttct gttttttgga ttttttgaat ctggtctaaa
      241 cagtaattag aagtataaat cggctcataa gcccctgacc agtcatttgg ataggaatag
      301 tctgccggtg tccaggtata gatttttttt ccataaacat caagctcatt ataagcttcc
      361 tctggcaaaa aataatcatc tgcagaggct tctccaaaat tggatgttgt ttgcaggttc
      421 atgcgggagg aataatccaa gagtgactga agatcattca gggttgtcgg cacggctacc
      481 gaactatccg gctttttact taaatatttg gtgcaggacg ggagcagact gccacttatt
      541 accaggataa ccactaggat aaccgtcttg tttggtttaa atctcataaa attccatttt
      601 gttgattaga aattggttct gattccaaag gcgaaagatt tcggcagggg attgccggaa
      661 ggatagtctg gatcaatctt tcccctggct gcatgccaga tcattcccag gttactcacg
      721 ttgaaataga tctggaaaag atcaaaggaa accacttcgt catttttgga agactgtact
      781 ccaggttaat gtattgtagc cggaagtggt ctcctttaag cgcatttatt tctgcatcct
      841 ggtaaaaacc atcccggctg gagtttgccg gatatataaa tgaaggcacc tgggtatgct
      901 tctcgtcacc aggcttttgc cagcggtctg catattcttt atttccgcct aaaccagaaa
      961 ccgtactgcc ataggaaaga gcagggcgca ggaaatagta gcccaatttg taggagatgt
     1021 tgacggaaac ggaaagctgc cggaaagaaa acgtattgat ccaggatccg aaatagacag
     1081 gaattgcaga tcccttatag tacaatgtgc cattggcaag tcctccttta aaggcttcat
     1141 tgcttatgcc tgaatagtct gtactcaact ggcctttaag gtatccccgg ggatttccca
     1201 ggctatccag gcctccccat ttataaacgg caatggcgta caacggcttt ccatttaccg
     1261 gcgctatttt tcttccggcc ccgcgaaagg tataaatact ccctgaagtt gatgcaaaat
     1321 atttatccgt cttgctgacg ttataattaa aaaggatctg actttcccat ttgaaccctt
     1381 tgtcaataac ccgggcattt aaaataacat ccattccatt ccctttcatg ctggctacat
     1441 ttttagtgat gatgtttgac ataccccagg tagtataatc aaagggcgtc ggtccgtaca
     1501 ggtcctttcc tctcttggaa tagtaatcca ccgatccttt caaccggcca ttttttgtgt
     1561 tgaagtcaaa acccaggttc aactgaccgg tctgttccca gcggagtccc gggttactcg
     1621 gactaataat gactgcattg gggaggctgg taatataatc cgaaccgaat tgggcaagcg
     1681 gcagggctga atttccgagg tcaacgtttc cgctataacc ataggtgatt cttaatttca
     1741 gaatgggcaa ccagcttaaa tggtaaaaat tttctttcga aggctcccag ctggcgccgg
     1801 ccgaccacag aggcttccat tggtcgtttg cctttacacc cagaatattc gacccgtctt
     1861 ttctggcgct ggcatacaga atatattttt ctttgtattt gtaagagaga ttgccaaata
     1921 aagaaataaa acggttttcc gtatgggaag acatactggg accggagatc gtttgaagac
     1981 caccggtaat cgttggataa tagttgacaa aatcaacatt ggcaaaagat agcggatccg
     2041 aatgataacc ataatagatg gagttgtccg cattgtcaac cacctcccgg atctcggcac
     2101 ccgccatggc ggttatatgc tggttggcaa gggacttatt gaaattgaat tgtcccctca
     2161 gattctggct tctttggacg ttctcgctta atcttaaaac atcacccagt ggtacaatat
     2221 aactgacaat gcctgtgctt ctatccacct ggctgaagag gttgatcgta ttccgggtat
     2281 cataactttg taggtctgaa aggttttccg ttccgccccg ctgggtgcta tactggtatt
     2341 ggatattgaa tttcagcggg tcgataatct taagatccag gccgatattc gccacaatgt
     2401 cttccacgcg ggaagtcgat ctggcgtgct tatactcatc caggggatag aatttccagt
     2461 ccagtagctt tcctccgcct gcagtatcag tatatgcact gcggaattgc tggggaactg
     2521 cggcgggtga tccgtcagga ttaaccaggt tggcgtacat gatgtgccgg ccgtttatcc
     2581 gggtagccgt agcatagtca ggtcttccca actcggattt gctgtttgta taatagacgc
     2641 cccagtcaag ctggatattt ctcccagcct tataactgtt attgaagcgg atatttattt
     2701 tttgaaacag ggcgccctgg ttatttaccg tccggtcgaa agcaccggag gcgagccagg
     2761 ccagcttatc ggttccccct cttaagctga ttgcgtgttg ctgcacaacc gcattctgat
     2821 aaaaatgatc catgtattgt tgtttgctgt cgattccctc gagggcattc accatagatg
     2881 cggaatctgc agaactgatc agcccgtttc ttttcttaag aaatacctca accgccgggg
     2941 gcagggcggt gtatttccgg ttgatggcag aattaaaata gcctttgctg aataagaatt
     3001 gttccattcc gatctcatcg gatacgctca tttccgggag ccggaagagg tcgggctttt
     3061 cggtaatgat caccccggaa ctgaaatcaa ttttagttcc ctggttgaac cgtccctttt
     3121 ttgtcgttat gacaataacg ccatttcccg cccgggaacc ccagatggaa gctgcggccg
     3181 catcttttaa aactgtgata ctttctatgt cgtttggatt gatattggcc aggttccctt
     3241 cgtaaatgaa attatccagt acgatcaggg gatccaaggg accgttgata gtgcttaatc
     3301 cgcggattga aatatttgtt ttattctcaa aatttccatt tccataaccg ggattgaacg
     3361 cgagtccgct ggttacgttc tgcagacggt tcaggatatc tgtccccgct tgctggttga
     3421 gcgttttgtt gtcgattaaa accacggatc cgttcatttc attcgattta accgactgat
     3481 acccggtgtt gacgacgacc acggaatcca ggctggaacc ggtagcatcc agtgatatgg
     3541 tcagctccaa agcaggtttc cggatcatta taaatttgga gtgatacccg atatgcgaaa
     3601 caaaaagcgt gtccggcaga tgggataatg atgcgctgaa cttaccggac gcatcgcagg
     3661 tcagggaaaa atggtttctc gtaaaggaga gggtggcgcc ggccagggtt tgcccgtctt
     3721 ttgaggatat tacggtcccc gagacaagcc cttcgctttg gggaatctgg gcaaatgaac
     3781 ctatagtcaa aaggatcata ttgccaatga tccagaaaat ctttttcatg ctgattcgtt
     3841 ttttggatta tttgtaccgg tcggctttaa atggaagatg gattttctct ccattcaata
     3901 cggcccggat atttatttcg gttatttctt ctgatgaggt tatcgccaat attttatgtt
     3961 ttcttccaat ccatatataa tggggaagaa acatgtgcgg aaacagaccg tacagatact
     4021 ttagcctttc tgtcgcaaac acaagtcttg aagcccacag aaattcattt ctttttagtt
     4081 ggaggaacga atcgactttt tctttactgt cgacggtgct ggtggaatcc tctatcagaa
     4141 taaacaacag cttttctttg aagcgggttt ggagggaatc tattttgggg aaataggata
     4201 aacagcttcc gcaccagatt gcccagaagt ccaggatgac taatttgttc ttcagatctg
     4261 ataaatggat tttagaaacg gggtagttgt gcaggttgct gattaggata tccggaaccg
     4321 gatcgccgat attcaacggt ttcgcctggg atggcggcga ctgtgcataa ccgttaacca
     4381 ccggccataa tatggccagc aagcaatttt ttaatgatct cattgcagtg tttcgttttg
     4441 atttttagga attcgtaaaa gacggccggt gggaaaaagg attacaggag aatgcagccg
     4501 gcgcaaaaga gctcgttaaa caggactctt gggcttacgg gcaaaaagaa tgattgaaag
     4561 cagtaaatag ggagaaaccg gaatgccggc agcgggcgcg ggaatggaag gggcattgat
     4621 gccgcctgcc tgggtaa
//
LOCUS       DASYCU010000241         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4835298, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000241 DASYCU010000000
VERSION     DASYCU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4835298"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VGM24_04870"
     CDS             <1..1151
                     /locus_tag="VGM24_04870"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEY4154731.1"
                     /translation="VKTFILYATGLLMFAFVYHYPYSRLFIFTSLCCFLLFILSTRII
                     IISASAYLDKVGRATRRIVIVGYNDVAKKLVQRLESTRKDFRVDGYFENQEQVHELST
                     LPIIGNISECIDYAISNHVQEIYSTISPEKDESIYEMSMVAEKSLIRFKFVPDFRLYV
                     NRVTYIEYLDNFPILSLRPEPLEDSGNAVKKRVFDVIFSLTVIVFLLSWLVPILAILI
                     KLTSRGPVFFKQSRSGRNNKQFTCYKLRTMKMNSEANLKQTTRNDPRVTWLGHLLRKT
                     NVDELPQFVNVLLGNMSVVGPRPHMLIHTNMYSKLMGEYMIRHFLKPGITGWAQVNGF
                     RGEIKDDEQLRGRVGHDIWYMENWSIWLDLKIILLTIYRTLKGDKKAF"
     gene            1148..>1555
                     /locus_tag="VGM24_04875"
     CDS             1148..>1555
                     /locus_tag="VGM24_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4154732.1"
                     /translation="MIRQHPVFFPKFFCSLVLLTVIGCAHAQSGSTAEKRFGFQAGIN
                     MTAMNFNLGSPPPPTTDKVVWKPGIVMGFLLRVSLAKDLFLQPEYAFTQRNGSDELLG
                     AGYRLDYLSMPLLLGYRIAPCISLLAGPQLELLI"
BASE COUNT          371 a          455 c          342 g          387 t
ORIGIN      
        1 cggtgaaaac ctttattctc tatgccacgg gtttgctgat gttcgccttc gtataccatt
       61 atccttattc aagactcttt atctttacca gcctctgttg ctttcttctt tttatccttt
      121 ccacacgcat catcatcatc agcgcctcgg cctatctgga taaagtgggt cgggctaccc
      181 ggcgcatcgt gatcgtgggt tacaacgatg tggcgaaaaa attggtgcaa cgcctggagt
      241 ccaccagaaa agacttccgg gtggacggct attttgaaaa ccaggaacag gtccacgagc
      301 tctctaccct gcccatcatc ggcaatatca gcgaatgcat cgactatgcc atcagcaacc
      361 atgttcagga gatctattcc accatctccc cggaaaaaga cgaaagcatc tatgaaatgt
      421 cgatggtcgc ggaaaaatcg ctgatccgtt tcaaattcgt cccggatttc cggctgtatg
      481 taaatcgcgt cacctatatc gaatacctcg acaatttccc catcctctcc ctgcgccccg
      541 aaccactgga agattccggc aatgccgtaa aaaagcgcgt gttcgacgtc atcttcagcc
      601 tcaccgtcat cgtttttttg ctgtcctggc tggttcccat cctggccatc ctcatcaaac
      661 tcacttcgcg cggacccgtt ttctttaaac aatcccgctc cggcaggaac aataaacagt
      721 ttacctgcta taaattgcgg accatgaaaa tgaattccga agcaaatctg aaacagacca
      781 cccgcaacga tcccagggtg acctggcttg gtcacctgct gcgaaaaacc aatgtcgacg
      841 aactgccaca gtttgttaac gtgctcctcg ggaatatgtc ggtcgtcggc ccccgccccc
      901 atatgcttat ccacaccaac atgtactcca aactcatggg cgaatacatg atccggcatt
      961 tcctgaaacc cggcatcacc ggctgggccc aggtgaatgg cttccggggc gaaataaaag
     1021 atgatgaaca actccgtggc cgcgttggcc acgatatctg gtacatggag aactggagca
     1081 tctggctcga tctcaaaatc attctcctca ctatttaccg caccttgaaa ggggataaaa
     1141 aagcgttttg atccgccaac accccgtatt cttcccaaaa tttttctgtt cgctcgtcct
     1201 gttgacagtc atcggctgcg cccacgcgca atcgggctca acagccgaaa aacgctttgg
     1261 ctttcaggcg ggcattaata tgaccgccat gaatttcaac ctgggcagcc caccgccacc
     1321 cacaaccgat aaagtggtct ggaagccggg tatcgtcatg ggttttttac tccgcgtttc
     1381 cctggcaaaa gaccttttcc tccagcccga atatgcgttt acgcaaagaa atggatcgga
     1441 cgaactgctg ggggccggct accgattgga ttatttgtcc atgccgctgc tgctgggcta
     1501 ccggattgct ccatgcattt ccctgttggc cggaccacaa ctggagctgc tgatc
//
LOCUS       DASYCU010000242         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3242734, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000242 DASYCU010000000
VERSION     DASYCU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242734"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2301)
                     /locus_tag="VGM24_04880"
     CDS             complement(<1..2301)
                     /locus_tag="VGM24_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY4154733.1"
                     /translation="MLTATSLFDGHDAAINIMRRIFQSKGVEVIHLGHNRSVQEIAEA
                     AIEEDVQAVAITSYQGGHIEFFKYLRDLLDESGCGHIRLFGGGGGTILPAEIKELQEY
                     GISRIYSPDDGREMGLEGMIEDVIRQTKRTFDPGEAPAGQFWEAGTRWNGTSPLGERK
                     DIRRIARSISLAENEFTQPDVKGAKPAPSNAPSSKIISSPVIGITGTGGAGKSSVTDE
                     ILRRFLQGSADKTIAVISVDPSKKKSGGALLGDRIRMNSIHSPRVYMRSLATRETDVA
                     LSAYVRDALDICRAAAFDLIILESAGTGQSDTSVLEYCDLSIYVMTPEYGAASQLEKI
                     NMLDYADLIVINKSDKPGAADALIEVRKQYKRNHGLWNAKEAELPVHATTASLFNDTG
                     TQHMFNELLKKLDQQCGTRFSSEIHLPEIKTHTEKSIIPAARVRYLSEIHESVQEYNA
                     LVKEQSRIATTLYQLNGALEETAKNLPDEKLSAGEDEGVKKKQAQENKNTADEKERDL
                     KTFLTRRISYYEAQLTPVSRKLIKNWPEKIKSYQQDQYRFNVRNQEITRSLFTKSLSG
                     TRIPKVVLPKYLDWGDILRWQAQENVPGSFPFTAGVFPLKRETEDPTRMFAGEGGPER
                     TNKRFHYVSAGQPFIRLSTAFDSVTLYGEDPDPRPDIFGKVGNSGVSICTVDDAKKLY
                     SGFDLCDPKTSVSMTINGPAPIILAFFLHAAIDQQCEKYISGNGLRDEVNRKIQEKFP
                     GGSRPVYQDAGSENLPEGNDGLGLKLL"
BASE COUNT          556 a          694 c          641 g          684 t
ORIGIN      
        1 aagcaacttc aatcccaatc catcgtttcc ttccggtaag ttttccgaac cggcatcctg
       61 gtaaacgggg cggctcccac cagggaattt ctcctggatc ttccggttca cttcgtcccg
      121 gagcccgttc ccggaaatat atttttcgca ttgctgatcg atcgccgcgt gcaggaaaaa
      181 agccagtatg ataggtgccg gcccgttgat cgtcatgctc acggatgttt tcggatcaca
      241 aagatcaaat ccgctgtata attttttcgc atcgtccacc gtgcagatgc ttacgccgct
      301 gtttcctact ttcccaaaga tatccggacg cgggtccggg tcttcgccgt ataaagtaac
      361 ggagtcaaac gccgtactca gtctgataaa tggctgtcct gcggaaacat aatgaaaccg
      421 cttattggtt ctttccggtc cgccttcccc ggcgaacatc cgggtcggat cttccgtttc
      481 ccgcttcaag ggaaaaacgc ccgccgtaaa gggaaaggac cccgggacat tttcctgtgc
      541 ctgccagcgc aggatatcac cccaatccag atatttcggg agaacgactt tgggaatgcg
      601 ggttccggat aagcttttcg taaaaagaga ccgggtgatc tcctgattgc ggacgttaaa
      661 acggtactga tcctgctgat aggatttaat tttctccggc cagtttttga tgagcttcct
      721 gcttaccggc gtgagctgcg cttcataata ggagatccgg cgggttaaaa aggttttcag
      781 gtcccgctcc ttttcatccg ccgtattttt attttcctgc gcctgttttt tctttacgcc
      841 ttcgtcttcg ccggccgata acttttcatc cggcaggttt ttggccgtct cctccagggc
      901 gccattcaac tggtataaag tggtcgcgat ccgcgactgt tctttcacca atgcattgta
      961 ttcctgaacc gattcgtgaa tctcactcag gtatcttacc cgggctgccg ggatgatcga
     1021 tttttcggta tgggttttta tctcgggcag atggatctct gaagagaacc gggtgccgca
     1081 ttgctgatcc agctttttta aaagctcgtt gaacatatgc tgcgttcccg tatcattgaa
     1141 taaggaggcg gtggtcgcat gcaccggcag ctctgcttct tttgcattcc agaggccgtg
     1201 attgcgtttg tactgtttgc gcacttcgat cagcgcatcc gcggccccgg gcttatctga
     1261 tttattaatg acgatcagat ccgcatagtc gagcatattg attttctcca gctgactggc
     1321 agcgccatat tccggcgtca tcacgtagat gctcagatca caatattcga gtacggacgt
     1381 atcactttgc cccgtgcctg cagattccag gatgatcagg tcgaaagcag ccgcacgaca
     1441 aatatccagc gcgtcccgga cataagcact gagcgccaca tctgtttccc gggtagcgag
     1501 cgaacgcata tagacccgcg gggaatgaat gctgttcatc cggatccggt cgcccaacag
     1561 cgcgccgccc gattttttct ttgaaggatc cacggaaatg acggcaatgg ttttgtccgc
     1621 ggacccctgc aaaaaacgac gcaggatctc atcggtcacg ctgcttttcc cggcaccgcc
     1681 ggtaccggtg atgccgataa ccggtgaaga aatgattttg gaggaaggag cgtttgatgg
     1741 agctggcttt gctcctttga catccggctg cgtgaattca ttttctgcca ggctgattga
     1801 ccgggcgata cgacggatat ccttgcgctc ccccagcggc gaagtaccat tccaacgggt
     1861 tccggcttcc cagaactggc cggcaggagc ttctccggga tcgaatgttc ttttggtttg
     1921 gcggataaca tcctcgatca taccctccaa tcccatctcc cgtccgtcgt cgggtgaata
     1981 tatccggctg atcccgtatt cctggagctc tttaatttcc gcgggaagga tggttccccc
     2041 accgccgcca aacaggcgga tatgtccgca accgctttca tccaacaggt cccggaggta
     2101 cttaaaaaat tcgatgtggc ccccctggta agaagtaatg gcaacggcct gcacatcttc
     2161 ctctatggcc gcctcggcaa tctcctgcac actccggtta tgcccgagat gaatcacctc
     2221 cactcccttg ctctggaaga tccggcgcat gatgttgatg gcggcatcat gcccgtcgaa
     2281 gagggaggtt gcggtcagca tcctcagcct ttgtgttgag ctattcataa agaaatcggc
     2341 tgataatcaa caaatttagc ttaaaaaact aatgagtgtt agttttggaa cggggcaatt
     2401 acggcgggtt tgggacaaga aaaggagggg agccgtgtta aataatttaa tgcttgcctt
     2461 taacaagagt gtccggctcg ttatcgcttc cataaatcag gctacttcta actgttttgc
     2521 gaaatggagt gcctattatt ctgcccacaa atctatatct aagggaatct cccgt
//
LOCUS       DASYCU010000243         5581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4835983, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000243 DASYCU010000000
VERSION     DASYCU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4835983"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1933
                     /locus_tag="VGM24_04885"
     CDS             <1..1933
                     /locus_tag="VGM24_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154734.1"
                     /translation="LIKYLHIIYRFCLVGLLVTLSPAVKARSLVTENILRDTIPKKEP
                     ASSIRKTRHGISKYLNIITDRQQRDSLLVKLSRQNEKAPVSDSVVFSRRENAFNVYRG
                     KFIRYIYFNQLKVFGTQIEDTVVASSRLIAFANRLHYNTRTWAIRQALFFKEGDTVNA
                     YKMVDNERYLRSLPFIQDARLYVINSYQKGDSIDIVVVTKDLYEYGGTVANISATRAS
                     ATVFNTNLLGAAQRVFLGFKWDKPYSPEWRTGFGYTKYNLAGTYADVAVGYSVLNDRP
                     ATDTGVYEKSSYISINRPLYSSWAKFTGGVTLSQNKSINIFNLPDTVYRNYQYSVVDV
                     WAGFNFRNQFKGTGYNSYKPNLAIELRRYDKDFSKRPDQHFLDSDPLYNDHQYLLSKF
                     VLFHQEFFKTNYFFGFGRTEDIPLGYNVSASFGFDKWTGRNRTYTAVEGQKYWFLGKN
                     LISANLALGSFWYNHASEDAVLHIQGDYYSNLFFLKNPKLREFLHADYIVCFNPVLYK
                     TVNINREEGILGYRYTLFNNFKRMNLSAQTNYYSPLNVYGFKFNFYIQVQASLLANEK
                     ESIFKSPFYSGFTMGCQIRNENLSFNTLQLSASYQPLVDHGPQAANGPRSLFVQITSI
                     TTFNFSIFALQAPSPIAFR"
     gene            2053..4065
                     /gene="tkt"
                     /locus_tag="VGM24_04890"
     CDS             2053..4065
                     /gene="tkt"
                     /locus_tag="VGM24_04890"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY4154735.1"
                     /translation="MMEVTEQTAAGIEQLCINTVRVLSADAVQQANSGHPGTPMALAP
                     VGHVLWSKMMNYNPQNPNWANRDRFVLSAGHACMLQYSYLHLTGYDITLNDIKKFRQL
                     HSKTAGHPEFGLLAGIEVTTGPLGQGFANGVGMAIAQKFLAARYNKPGYDLFDYHIYA
                     ICSDGDMMEGISSEAASIAGNLQLGNLIYIYDDNHITIEGDTALTFNEDVSGRFLAYH
                     WHVQELPDGNDVEAIAEALKKAKEEKTRPSLIRIRTHIGYGSPNKEDTAEAHGSPLGA
                     DEVKLVKQFFGFDPDQFFVIPDKVLSFYREAGKKGIEKEKQWNNGFKQYKEKYPDEAN
                     EWVTISSGKLPSGWKELLPSFKGPDKMATRKASGKVLNAIADKLPFLIGGAADLSPST
                     DTILQKYASFSSQQRDGRNFHFGIREHAMAAILNGIAVTKGFIPYGATFLIFSDYMRP
                     AIRLAAISKIRPVYVFTHDSIGLGEDGTTHQPIEQLVSLRAIPNMTVIRPADANETAH
                     AWRVALEIQSGPVAIVLTRQGIPVIDQEKYAKASGLEKGAYILSESAEPPGVILIGTG
                     SEVSLLLKAQEALAQKSIAARVVSMPSWELFEKQDAAYKESVLPKAIRKRLSVEAGSP
                     QGWLKYTTEDGKILAMHRFGESAPGEEVFKEFGFTVENVVALAASL"
     gene            4079..5146
                     /locus_tag="VGM24_04895"
     CDS             4079..5146
                     /locus_tag="VGM24_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4154736.1"
                     /translation="MNHLMKLPFYARLALTLFAVALVLLFMWMGKSILIPLFFAFLIS
                     ILLHPVVRFFEKHRFPRALASLLTILIFLILIAGLFYFFSRQVNRLSKDLPAIQARIS
                     AQVQGIQDWIYNKYHINNSQQEAYANKTVNGVLNTAVNSAATTFVGIAETLILSIFIF
                     VFTFFILFHRRLLMRFLLALFDEEHNKRVSIVVSKVRLLINNYVLGLLMEMLVLMILI
                     FTSLMIIGIKYALLLAVIAAILNIIPYFGIYISMAIAVLVTMSNGSGGSAITVLIVFL
                     AAHFIDANVVLPRIVGGQVKMNPFITLLAVLIGHLIWGIPGMFLFVPLTAIIRLISEE
                     IPEMNPWAILIGEEKYSKKKK"
     gene            5269..>5581
                     /locus_tag="VGM24_04900"
     CDS             5269..>5581
                     /locus_tag="VGM24_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154737.1"
                     /translation="MTISEKVKKIFRRIFRITLVSVLVVFSVILLILLLIQTSPVQNY
                     GRQKIEAYLEKKLQTRVRIGKLYIGLPSKLILKNIYLGDQQKDTLLSGGNIEVDISML
                     KL"
BASE COUNT         1403 a         1348 c         1386 g         1444 t
ORIGIN      
        1 tttgataaaa tacttgcata tcatttaccg cttttgcctg gtgggcctgc tggtcaccct
       61 gagcccggcc gtaaaagcgc gcagtcttgt tactgaaaac atcctccggg ataccatccc
      121 gaagaaagag cccgcttcct ccatccggaa aacacgccat gggatttcga agtatttaaa
      181 tatcatcacg gaccggcaac aaagggactc cctcctggtc aagctttcca gacaaaatga
      241 aaaagcgccg gtctccgaca gtgttgtttt cagccgccgg gaaaatgcct tcaatgtata
      301 ccggggcaaa ttcatccggt atatctattt caaccagctc aaggtattcg gcacccagat
      361 cgaagacacc gtggtagcgt cctcccggct gatcgctttt gccaaccgcc ttcactataa
      421 taccaggacc tgggctatcc gccaggcttt gttctttaag gagggggata ccgtgaatgc
      481 atataagatg gtcgataatg agcgctacct gcgtagtctt ccctttatcc aggatgcccg
      541 actgtatgtg atcaacagtt accagaaggg ggattccata gacattgtgg tggtaacaaa
      601 agatctgtat gagtatgggg gaaccgttgc caatatcagt gcaacccgtg cttctgccac
      661 ggtcttcaac accaatttgc tgggtgcggc gcaacgggtg tttctgggtt ttaaatggga
      721 taagccttat tctcccgaat ggcgtaccgg ctttggatat accaaataca acctggcggg
      781 aacctatgcc gatgttgccg tcggttacag tgttttgaac gaccggccgg ccacggatac
      841 gggggtgtat gaaaaatcga gttatatcag catcaaccgg cccctctaca gttcctgggc
      901 aaaattcacg gggggcgtca ccctctcaca gaacaagtcg atcaatattt ttaatttacc
      961 ggatacggtg taccggaatt atcaatacag cgtggtggat gtttgggcgg gtttcaattt
     1021 ccgcaaccag tttaaaggaa ccggatacaa cagctataag ccgaacctgg cgatcgaact
     1081 caggagatat gataaagatt tttcaaagag accggatcag cattttctgg attcggaccc
     1141 gttgtataat gatcaccagt acctgctcag caaatttgtg ctctttcacc aggaattctt
     1201 caagaccaat tattttttcg ggtttggccg cacggaagat attccgcttg ggtacaatgt
     1261 ttccgcttcc tttggctttg acaaatggac cggcaggaac cggacctata cggcggtgga
     1321 ggggcagaag tactggtttc tgggaaagaa cctgatcagt gctaacctgg cgctgggatc
     1381 tttctggtac aaccatgctt ccgaagatgc cgtgctgcat atccaggggg actattacag
     1441 caatctcttt tttttgaaga atcccaagct cagggaattt ctccacgcag attatatcgt
     1501 ctgctttaat ccggtcttat acaaaacggt aaacataaac cgagaagaag gaatcctggg
     1561 ttaccggtat acgctattca ataattttaa gcggatgaac ctgagcgcgc agaccaatta
     1621 ctatagtccg ctgaacgtgt atgggtttaa atttaatttt tatatccagg tgcaggcttc
     1681 cctgctggcc aacgaaaagg aaagcatttt taaaagccct ttttattcgg gctttaccat
     1741 gggctgccag atccggaatg aaaacctctc gttcaacacc ctgcagctct cggccagcta
     1801 ccagcccctg gtggatcatg gaccgcaggc ggctaatggt ccccggagcc tgtttgttca
     1861 gatcacctcg ataaccactt ttaatttcag catttttgcc cttcaggcgc cttcacccat
     1921 tgccttccga tgatccggcg tgccgacaat aaagtatctt tattaaagaa aacgggtgag
     1981 gaaaataata ctcaccccaa ttgtcttcac aatggaatgg tttatgaagt ttaaacataa
     2041 aaataatatg ccatgatgga agtaactgaa caaacggctg ccgggatcga acaactttgt
     2101 atcaataccg taagggtact gtcggcggat gcagttcaac aggcaaattc cggccatccc
     2161 ggaaccccca tggcgctggc tccggttggt catgtgctct ggtcaaagat gatgaactac
     2221 aatccgcaga atccgaactg ggccaaccgg gaccgttttg ttttgtcggc cggacatgcc
     2281 tgcatgctgc agtatagcta cctccatttg accggatacg atatcacgct gaacgatata
     2341 aaaaaattcc ggcagttgca cagtaaaacg gcgggccatc ccgaattcgg attgctggcg
     2401 gggatagaag tcactaccgg tcctttgggt cagggctttg ccaacggagt ggggatggcc
     2461 atcgcgcaga aattcctggc ggcgcgctat aataaacccg gttatgatct gtttgattac
     2521 catatttacg cgatttgcag tgatggcgat atgatggaag gaatttcttc ggaagccgct
     2581 tccattgccg ggaacctgca gctcggaaac ctgatttaca tatacgacga taaccatatc
     2641 accatcgagg gggatacggc gctgacattt aatgaagatg tttccggaag gtttcttgcc
     2701 tatcactggc atgttcagga attgccggac ggaaatgatg tggaagccat cgccgaagcc
     2761 ctgaaaaaag caaaggagga aaagacgagg ccttcgctga tccgcatccg cacgcatatc
     2821 ggctatggaa gtccgaataa ggaagacact gccgaagcac atggttcgcc gctgggcgcg
     2881 gacgaagtga aactggtaaa acagtttttc ggatttgatc cggaccagtt ttttgttatt
     2941 ccggacaaag tgctttcttt ttaccgggag gccggaaaaa aaggaattga aaaggaaaag
     3001 caatggaaca acgggttcaa acagtacaag gaaaaatatc cggatgaggc caatgagtgg
     3061 gtcaccatca gcagcggcaa acttccttcc ggctggaagg aattattgcc gtcctttaaa
     3121 ggaccggaca aaatggcgac ccgcaaggca tcgggaaaag tactgaatgc cattgcggat
     3181 aaattgccat ttctgatcgg gggagcggcg gatctgtccc catccaccga taccattctg
     3241 cagaaatacg cttccttttc ttcccagcag cgggacggcc ggaattttca ttttggaatc
     3301 cgggaacacg ccatggcggc tattctgaat ggcatcgccg tgacgaaagg atttattccc
     3361 tacggagcca cctttctcat cttttcggat tatatgcggc cggctatccg gctggcggcc
     3421 atcagcaaga tccggcctgt ttacgtattt acacacgaca gtatcggact gggcgaagac
     3481 ggaactacgc atcagcccat agaacaactg gtctctttaa gggccattcc caacatgacg
     3541 gtcatccgtc cggcagatgc aaatgaaacg gctcatgcct ggcgtgtggc attggaaata
     3601 caatccggcc cggtggcgat tgttttgacc cggcaaggga taccggtcat tgaccaggaa
     3661 aaatatgcca aagcatcggg cctcgaaaaa ggggcttata ttttgtccga atctgcagaa
     3721 ccgcccggag ttatcctgat cggaaccggg tcggaggtca gcctcttgtt aaaggcccag
     3781 gaagccctgg cgcaaaaatc cattgccgcc cgcgtggtga gcatgccttc ctgggagtta
     3841 tttgaaaaac aggatgcggc ctataaggaa tccgttcttc ccaaagcgat ccgcaaaagg
     3901 ctttcggtgg aagccggttc tccgcagggc tggttaaaat ataccacgga agatggaaag
     3961 atcctggcca tgcaccgttt cggcgaatcg gctccgggag aagaggtttt caaagagttt
     4021 ggatttactg ttgagaatgt cgttgcatta gcggcttctt tataacccgg cgttatctat
     4081 gaaccacctg atgaagcttc cgttttatgc ccggctggcg ctaaccctgt ttgctgttgc
     4141 cctggttttg ttgtttatgt ggatgggaaa atcgatttta attcctttgt tttttgcttt
     4201 tttaatttcg atcctgttgc atccggtggt gcgctttttt gagaagcacc ggtttccccg
     4261 ggcactggct tcgctgttga ccatcctgat ctttctgatc ctgattgcgg ggttgtttta
     4321 ttttttcagc aggcaggtca accggctttc gaaagatctt cccgccatcc aggccaggat
     4381 ctcggcgcag gtacagggca tccaggactg gatctataac aaataccata tcaacaattc
     4441 gcaacaggag gcgtatgcga ataaaacggt caacggcgtg ctgaacaccg ccgtgaattc
     4501 ggcagccacc acatttgtcg gtattgcaga aaccctgatc ctgtcgatct tcatttttgt
     4561 ttttactttt tttatcttat ttcaccgccg gctgctcatg cgctttttgc tcgcgttgtt
     4621 tgacgaagag cataataagc gcgtaagcat cgtcgtgagt aaggtgcgcc tgctgatcaa
     4681 caattacgta ctgggcctgc tcatggagat gctggtgctg atgatcctga tttttaccag
     4741 cctgatgatc atcgggatca aatatgcgtt attgctggcg gtgattgcgg ccatactgaa
     4801 tatcatccct tatttcggca tttatatctc catggccatt gccgtactgg taacgatgtc
     4861 caatggttcc ggcggaagcg ccattacggt cctcattgta ttcctggccg cgcattttat
     4921 cgacgccaat gtcgtactgc cgcgcatcgt gggaggccag gttaaaatga atccgttcat
     4981 tacgctgctg gccgtgttga tcggtcacct gatctggggc ataccgggca tgtttttatt
     5041 tgtgccgctg acggcgatca tccgtctcat cagtgaggaa attcccgaaa tgaatccatg
     5101 ggcgattttg atcggtgaag agaaatattc aaaaaagaag aaatagccgg ataccctttg
     5161 tagatttttt tccccggtgc ctgccggtta cctttgcaaa ctacggccgg aagaagtttt
     5221 taaaaaaggc ctgttctttg ccttaaacag gtaacgcagc caaacaccat gaccattagc
     5281 gagaaagtga aaaagatctt ccggcgcatt tttcgtatta ccctggtatc cgttctggtt
     5341 gtcttcagtg tcattctcct gatcctgtta cttattcaaa caagtccggt tcagaattac
     5401 ggccgccaga aaatagaagc ctaccttgaa aaaaagctgc agacccgggt tcgcatcggc
     5461 aaactctata tcggcctgcc ctcaaagctg atcctgaaga atatttatct cggtgaccag
     5521 cagaaggata ctttattatc cgggggaaat attgaagtgg acatcagcat gctgaaactt
     5581 c
//
LOCUS       DASYCU010000244         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_200269, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000244 DASYCU010000000
VERSION     DASYCU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200269"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VGM24_04905"
     CDS             <1..429
                     /locus_tag="VGM24_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4154738.1"
                     /translation="QEVLVEANNATLAFPDRTNTDHFKKYKLVNTYLEVPLELRYAFN
                     PENTNKSWKLALGVKPGVMLSAYTKGKNLLNSAGQLEQAYIEKNSSKRFFNGTRVAGT
                     VRVSYGFFGVFGQYQLTSLIKSGSGPAINPYSIGIVLSGL"
     gene            499..1689
                     /gene="hflX"
                     /locus_tag="VGM24_04910"
     CDS             499..1689
                     /gene="hflX"
                     /locus_tag="VGM24_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY4154739.1"
                     /translation="MAVEQHIMQPEEKAVLVAVMQPDQTERQVKEYLDELAFLAETAG
                     AKTVRQFMQKLAHPDSKTFVGKGKLEEIRNFVQGKDIRVVIFDDELSGSQITNIEKVL
                     DTKTIDRSDLILDIFARRAKTAQARLQVELAQYQYLLPRLRGMWKHLERLGGGIGTRG
                     PGETEIETDRRIVRDKITLLRKKLREIDRQAFTQRKERGELIRVALVGYTNVGKSTIM
                     TLLSKSDVFAENKLFATLDTTTRKIVYENTPFLLSDTVGFIRKLPHHLVESFKSTLDE
                     VREADLLLHVVDISHPQYEEQIGVVNNTLQELKAFDKPVIYVFNKMDRYEEDSFDPWL
                     ESGVKEELLRELMERWSAETRSTCVFISATAKRNIDGLRQALTDQVKILYQERYPYRT
                     DNFY"
     gene            1806..1879
                     /locus_tag="VGM24_04915"
     tRNA            1806..1879
                     /locus_tag="VGM24_04915"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1840..1842,aa:Asn,seq:gtt)
     gene            2423..>2997
                     /locus_tag="VGM24_04920"
     CDS             2423..>2997
                     /locus_tag="VGM24_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154740.1"
                     /translation="METGICQLCLQRKELLKKAHIIPQFMYKGMRNAQNQMVSRKLSE
                     LSAKGKLVQTGFYEKSILCADCDNRILGRLDDYADGLLYGKGLIIRKDGLSPDGQIRM
                     LNLQGIDYKKFKLFLLSVLWRAHISKNKFFENVDLGAEADKIRHIILNGIEVDESTYK
                     IVMFAIMLNERELARIVVTPRHINENGLNFCT"
BASE COUNT          864 a          619 c          771 g          743 t
ORIGIN      
        1 caggaggtgc tggtggaagc caacaatgcc acgcttgcct ttcctgaccg gaccaatacc
       61 gatcatttca aaaaatacaa actggtaaac acttacctgg aagtgccttt ggaactccgg
      121 tatgcgttta acccggagaa taccaataaa agctggaagc tggccctggg cgttaagccg
      181 ggcgtgatgc tgagcgccta tacgaaggga aagaatttac tgaattcggc cgggcagctg
      241 gagcaggcct atatagaaaa aaacagttcc aagagattct ttaacggcac ccgggtagcg
      301 ggcacggtcc gcgtcagcta cggctttttc ggtgtcttcg ggcaatatca gctcaccagt
      361 ttaatcaaaa gcgggtcggg cccggcaatc aatccctatt ccatcggcat tgtcctgagc
      421 ggattataag gaaggatctt tgctccggaa tccatcccgt tcaccgggcg tcttatcttt
      481 agaaaaaata attatcattt ggcggtagag caacatatca tgcagccgga ggaaaaggct
      541 gttttagtcg cggtaatgca acccgatcag acggaacgcc aggtaaaaga atacctggat
      601 gagctggctt tcctggccga gacggcggga gcgaaaacag tccggcagtt catgcaaaag
      661 ctcgctcatc cggacagcaa aacatttgtg ggtaaaggca aactcgagga gatccggaat
      721 tttgtgcagg gaaaggatat ccgggtggtg atcttcgatg atgaattatc ggggtcccag
      781 atcacgaata tcgaaaaagt gctggacaca aaaacgatcg atcggtcgga tctcatcctg
      841 gatatttttg cccgccgcgc aaaaacggcg caggcgagac tgcaggtgga gctggcgcag
      901 taccagtacc tgctgccgcg gcttcgcggg atgtggaagc acctggaaag gctggggggc
      961 ggtatcggaa cgcgcggtcc gggggaaacg gaaattgaaa cggaccggcg tattgtgagg
     1021 gacaagatca cgctgctccg caagaagctg cgggagatcg accggcaggc attcacgcag
     1081 cggaaagagc gcggcgagct gatccgcgtt gcgctggtgg gctatacgaa tgtggggaag
     1141 tccacgatca tgacgctgct gagcaagagc gatgtattcg ccgagaataa attatttgcc
     1201 accctggata cgaccacgcg gaagatcgtg tatgaaaaca cgccctttct cctgagcgat
     1261 acggtaggat tcatccggaa acttccgcac catctcgtgg aaagtttcaa gagcacgctg
     1321 gatgaagtgc gcgaagcgga cctcctgctg catgtggtgg acatttcgca tccgcaatat
     1381 gaagagcaga tcggtgtggt gaacaatacg ctgcaggaat taaaagcctt cgacaaaccg
     1441 gtgatctatg tattcaataa aatggatcgc tacgaagaag acagtttcga tccctggctg
     1501 gaatcggggg tgaaagagga gcttctgcgg gagctcatgg aaagatggtc ggcagaaacg
     1561 cgctcgacct gcgtattcat atcggcgacg gcgaagcgga acatagacgg cttgcgccag
     1621 gccctgacgg accaggtgaa aatactttac caggagcgct atccctaccg gacggataat
     1681 ttttattgat cgccgaatgc gcctgcccgg aaaaaatatt ccggccaaac tttcctgctt
     1741 tcagattttg ccaaaggagg aaaaagctta tttttgcagc ccttccgtta aacggaagca
     1801 gatactcccc tttagctcag ttggttagag catctgactg ttaatcagag ggtcgctggt
     1861 tcaagtccag cagggggagc tgattttcaa tcttttacaa ggtcgccaat ggcggccttt
     1921 ttttatttgc cagtaatttg ccagcagaat ttttagcaat gaagcaaaga gctgtttact
     1981 ggataaaggt atttcatttt tatctatttt ttttcagtag ctgaactaaa tcaacttcaa
     2041 aaaagtgagc tttatatttt cgtaaaatga attataagcc ggagaaggta tttgcttacc
     2101 tcgtaattcc ttaatcgagt gatttgcggt caagctatag agtgaaattt cagtcaagca
     2161 agaattttac cgcgtccttc ttgttttaaa tatcaatcgc attaaacatg ggagccttct
     2221 tacaaatata aagaaggtgt atttgagtta ggttctatta ttaccgtatc ggacttcata
     2281 attatttgct tcatttcatc caacccacca accccgtata ggctgtatca atggcggatg
     2341 gcctatggaa gtacaggatg gaattgcttg ggtattggcg gaacccatta tagcctggta
     2401 tgaaaaagat taaattgcat aaatggaaac aggcatttgc caattgtgtc ttcaaagaaa
     2461 agaattatta aaaaaggcgc atataattcc gcaattcatg tacaaaggaa tgcggaatgc
     2521 ccaaaatcaa atggtcagcc ggaaattatc tgaattatcg gccaaaggga aattggttca
     2581 gactgggttt tacgaaaaat cgattttatg cgccgattgt gacaatagaa tattgggcag
     2641 actcgatgat tatgccgatg gtttacttta tggaaaagga ttgattattc gtaaagatgg
     2701 ccttagtcca gacggccaaa taaggatgct gaatttgcaa ggaattgatt ataaaaaatt
     2761 caaattgttt ttgttatctg ttttatggcg cgcacatata agtaagaata aattttttga
     2821 aaatgtggat ttgggcgcag aagccgacaa gataagacac ataattctta acggcatcga
     2881 agttgatgaa tcgacatata aaatagttat gtttgccata atgcttaatg aaagggaatt
     2941 ggcgagaata gttgtaacgc cacgacatat aaacgaaaat ggattgaact tctgcac
//
LOCUS       DASYCU010000245         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_200307, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000245 DASYCU010000000
VERSION     DASYCU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200307"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..>2376
                     /locus_tag="VGM24_04925"
     CDS             143..>2376
                     /locus_tag="VGM24_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4154741.1"
                     /translation="MENLIASEQVAAENLSFEHFREEVLNDFRLVCESREASLLGRKE
                     VLTGKAKFGIFGDGKEVPQVAVAKFFRPGDFYAGYYRDQTLGFALGTSTVEEFFSQLY
                     ADPDILHDPHSGGRNMNAHFASPLVGEDGQILDLVNRKNFTAGLAPTAAHIPRSLGLA
                     YASKLFREVKELKDLKNLSVNGNEICFCTIGDASTSEGHFWEIINAAGVLQIPLAIFV
                     WDDGFGISVPVKYQTTKGSISEVMKGFQKKEGSNGIDIYKVKAWDYAGMCETFESAIH
                     KIRVTHTPALFHVEEVTQPQGHSTSGSHERYKAPERLEWERSWDAMKKMKEWIIENAL
                     ASLEEIDEIHAKAKETVRNSKIAAWEKCMSPIRQQVSKSLALIRDLMPALPGKTAQVE
                     KIIQEMSLLREPNRRDVVSGIHRVLRLTGNRPQAASLRNYYEELNRENSAFYNTHLYH
                     EGAKKIGNIRAVPARYGEDAPLLNGYEILNKYFDILFEHNPKVLAFGEDLGQIGDVNQ
                     GFAGLQLKYGEGRISDTGIREVAIIGKGIGLAMRGLRPIAEIQYIDYLLYAIQTLSDD
                     LATLQYRTAGKQTAPLIVRTRGHRLEGIWHSGSPMGMIVNSLRGMHVCVPRNMVQATG
                     MYNTLLEGMDPGLVVECLNGYRLKERLPSNLLEFRVALGMPEVIREGTDMTIVSYGST
                     LRIIADAANSLEELGISCEIIDVQTLLPFDTEHRILQSLKKTNRIIFIDEDVPGGAAA
                     YMFN"
BASE COUNT          605 a          578 c          652 g          541 t
ORIGIN      
        1 tattaacaac cccccctttt tcatagtttg taaagaaatc tgtcctaaaa ttacatagta
       61 aacgtcacct ttgccaataa attttggggc atctattttt ttaaccttgc cttaggggat
      121 cacccgttta aacggatcaa atatggaaaa tctgatcgcc agtgaacaag tggctgctga
      181 aaatctttct tttgaacact tccgggaaga ggtcttaaat gatttccgtt tggtttgtga
      241 aagccgggaa gccagccttc tcgggcgcaa ggaagtgctt accggaaaag ccaaattcgg
      301 tattttcggg gatggcaagg aagtgcccca ggtggcagtg gccaaattct tccggccggg
      361 tgatttttat gccggttatt accgggacca gaccctgggc tttgcgctgg ggacctctac
      421 cgttgaagaa ttcttctccc agctttacgc cgatcccgat attcttcacg atccgcatag
      481 cggaggccga aatatgaatg cccatttcgc ttctccgctg gtcggagagg acgggcagat
      541 cctggacctg gtcaaccgga agaactttac ggccgggctg gcgccgactg ctgcccatat
      601 tccccgttcc ctgggtctgg catatgcatc gaaattattc cgggaagtga aagaactgaa
      661 ggacctgaaa aacttatcgg ttaacgggaa tgagatctgt ttttgcacca tcggggatgc
      721 gtccaccagc gagggccatt tctgggaaat catcaacgcg gccggcgtac tccagattcc
      781 cctggccatt tttgtctggg acgacggttt cgggatttcg gtaccggtta aatatcaaac
      841 caccaagggt tcgatttcgg aagtaatgaa aggctttcag aaaaaggaag gcagcaacgg
      901 gatcgatatt tacaaagtaa aagcctggga ttatgccggc atgtgcgaga cctttgaatc
      961 ggccatccac aagatccggg tcacccacac accggccctt tttcatgtgg aagaagttac
     1021 gcagccccag ggccattcca cttcgggtag ccatgaacgg tataaagcgc cggaaaggct
     1081 ggaatgggaa agatcctggg atgccatgaa aaaaatgaag gagtggatca ttgaaaatgc
     1141 gctggcgagc ctggaagaga tcgatgagat tcacgcaaag gccaaggaaa ctgttcgtaa
     1201 cagcaagata gcggcctggg aaaaatgcat gtcacctatc cgtcagcagg taagcaagtc
     1261 ccttgccctg atccgggacc tgatgccggc cttaccggga aaaaccgcgc aggtggaaaa
     1321 aatcatccag gagatgagcc tgctccggga acccaatcgc cgggatgtgg taagcggcat
     1381 ccaccgggtg ctccggctaa cgggaaaccg gccgcaggcg gcttcattac ggaactacta
     1441 tgaagagctg aaccgggaaa acagtgcatt ttacaatacc catttatacc atgaaggagc
     1501 aaagaaaatc ggaaatatcc gcgcggtgcc cgcacgatat ggggaagatg caccgctact
     1561 caacgggtat gagatcctca ataaatattt tgacatcctg tttgaacata acccgaaagt
     1621 gctggctttt ggggaagacc tcggccagat cggggatgtt aaccaggggt ttgcggggct
     1681 gcaattaaaa tacggggaag gccgtatttc ggatacgggt atccgtgagg tagccattat
     1741 cggtaagggc atcgggctcg ccatgcgggg tctccggccc attgcagaaa tccaatacat
     1801 cgattatctt ctttatgcca tacagaccct gagcgatgac ctcgccaccc tgcaataccg
     1861 gacggcagga aagcaaacgg cgccgttaat tgtgcgtacg agaggccacc ggctggaagg
     1921 gatctggcac agtggctctc cgatgggtat gatcgtcaat tccctgcggg ggatgcatgt
     1981 ttgtgttccc cggaatatgg ttcaggccac cggcatgtat aataccctgc tggaaggaat
     2041 ggatccgggc ctggtggtgg aatgtttgaa cggttaccgc ctcaaggaac ggctgccctc
     2101 caatctgctg gaattccggg ttgcactggg tatgccggag gtcattagag aggggaccga
     2161 tatgaccatt gtgtcctacg gatccaccct tcgcatcatt gccgatgcgg cgaacagcct
     2221 ggaagaactg ggtatcagtt gcgagattat cgacgtgcag accctcctgc cttttgatac
     2281 ggagcaccgt attctgcaat cgctgaaaaa aaccaaccgc attattttca ttgatgaaga
     2341 tgtgccgggg ggcgccgcgg cctatatgtt caataa
//
LOCUS       DASYCU010000246         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4695281, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000246 DASYCU010000000
VERSION     DASYCU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4695281"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VGM24_04930"
     CDS             complement(<1..819)
                     /locus_tag="VGM24_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4154742.1"
                     /translation="MKELTQTDVVVLGGGASGIAAAVAAAKSGLQVALIERNPFFGGS
                     ATAAEVGTICGLYKFSRKKSSEYIVGRFAKDFAERLKIKSGSEPLFNSSGLHYLPYHI
                     EAFKKICSNLLDEHKVERFLNTELHSLNRSDTKIESVSVNTEGESFRFTMHSVIDCSG
                     DGRLSQLADLPLIKSERYQAAAQVFTLQNLTETDEAILGFVLMKELRRAIKEKKLPHY
                     FDRVYVVPGSLTDHCASFKIGLPLEVTHTPENLHELKSVAHSFIHELTAYLVQQV"
     gene            complement(816..2135)
                     /locus_tag="VGM24_04935"
     CDS             complement(816..2135)
                     /locus_tag="VGM24_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154743.1"
                     /translation="MRPFDKSILPEKVLLSGSDCFHRVLDKHARIHHSGSNVMRIVFY
                     FNNRLPVDKIKRAFSNSPLIYWLCNIKLKKGLLFDIPHWRYRDRGNGMVINEYHYAVP
                     NEIPETISKRDIPLDGERFVEADIICYPGNRTAFVLSWNHILMDVKGIAMLIHHLNEL
                     NCGNDNYQIKLLFPGPEKKIGLFSHIKNMYAVKKFIEASSKAPIASIAGKNSQNTATF
                     KNRILHFTAEETDAIHEHAIQNGAGLGDNPYYLSCCCLAVNNILKQRQHGGVLWIPIP
                     YDGRQKGSLGPVISNTVAFIFYRIPEKALTSMKETVTCFSTQMTEQIKEKMPRKYGLL
                     LNLMRHLPLRLYYFLVNKSGEGNFASFLYSSAGETFNDLKTLFGETISTLSIFPSPTS
                     PPGLTFSFQKHRGALNINMAYSLDIVDSPELNKIGIDLKHFLMGINE"
     gene            complement(2148..2393)
                     /locus_tag="VGM24_04940"
     CDS             complement(2148..2393)
                     /locus_tag="VGM24_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY4154744.1"
                     /translation="MDENIIIGDIKEFIEVNILAGNIKLDAETILQNAGIDSFSMIEI
                     LLFVQEKYNMLVPDDQLLPENFKTLASLSRLVNRLSV"
     gene            complement(2397..>2750)
                     /locus_tag="VGM24_04945"
     CDS             complement(2397..>2750)
                     /locus_tag="VGM24_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154745.1"
                     /translation="FWGIAILTLATALAATGYGVLVGTLASTEQQGAIMGSLSILLLS
                     ALGGIWVPTYVMPESMRSISAFSPLNWSLDGYYKLFLRGSGAWAILADAMKLVIFSIA
                     TMAISSFTNHIKREV"
BASE COUNT          737 a          610 c          618 g          785 t
ORIGIN      
        1 tacctgttgt accaaatacg ctgttaattc atgaataaaa gaatgagcaa cgctttttaa
       61 ttcatgcagg ttttcgggcg tatgcgtgac ttccagcgga agaccaattt taaagctcgc
      121 gcaatgatcc gtcagcgatc ccggaaccac ataaacccgg tcaaaatagt ggggtaattt
      181 tttttcttta atggcccggc gcaattcctt cattaaaaca aatcccaata tggcttcgtc
      241 ggtttcggtc agattttgca aagtaaatac ttgtgcggcc gcctggtaac gctcgctttt
      301 aatcaggggg agatcagcca gttgactcag ccggccatcc cctgaacaat caatgacgga
      361 gtgcatggta aaccgaaagg attcgccttc tgtattaaca gaaacggatt caatttttgt
      421 atcggaccgg tttagactgt gcagttcagt attgagaaat cgctccactt tatgttcatc
      481 caacagattg ctgcatattt tcttgaaagc ctcaatatga taaggaagat aatgcagacc
      541 ggaggaatta aataatggtt ctgatccgga tttgattttc aggcgttcgg caaaatcttt
      601 cgcgaaccgg cctacgatgt attctgatga ttttttcctg ctgaatttat acaggccaca
      661 aatcgtacct acttccgcag cggtcgctga tcctccaaaa aaaggatttc tttctatcag
      721 cgctacctgt aacccggatt ttgctgccgc tacggcagca gctatcccgg aagcgccgcc
      781 gcccagtaca acgacatccg tttgtgttaa ttccttcatt cgtttattcc cattaaaaaa
      841 tgtttcaggt caatcccgat tttatttagt tcagggctat ccacaatatc cagggagtag
      901 gccatattga tgtttaatgc tccccggtgt ttctggaagg aaaaagtcaa tccgggtgga
      961 gatgtgggcg aaggaaaaat gctgagggtg ctgatggttt ctccaaacag ggtttttaaa
     1021 tcattgaatg tttctccggc tgatgagtac aagaaactcg cgaagttgcc ctcaccgctt
     1081 ttgttcacca aaaagtaata caggcggagc ggaagatgtc gcatcaggtt gagcagcaag
     1141 ccatatttcc ggggcatttt ttcttttatc tgctccgtca tttgggtgga aaagcaggtc
     1201 accgtctctt tcatactggt taaggccttt tccggaatcc gataaaatat aaaagcgact
     1261 gtattggaga taaccggacc cagagatccc ttctgacgac catcataggg tatcggaatc
     1321 cacagtacgc ccccatgctg tctttgcttt aaaatattat tgacggcaag gcagcaacag
     1381 cttaaataat aaggattatc gcccaatccc gctccgtttt ggatggcatg ttcatggatt
     1441 gcgtccgttt cttcggcggt aaaatggagt atcctgtttt tgaatgtggc tgtattttgg
     1501 ctgttttttc cggcgatgga cgcgatgggc gctttggagg aggcttcaat aaatttttta
     1561 accgcgtaca tattttttat gtgactgaac aggccgatct ttttctccgg accgggaaaa
     1621 agtaatttaa tctggtaatt gtcgtttccg cagttcagtt cattgaggtg atgaataagc
     1681 atagcgatcc ctttcacatc catcaggata tgattccacg ataaaacgaa tgccgttcta
     1741 ttgccggggt agcagataat atcggcttct acgaatcttt cgccgtcaag gggtatatct
     1801 ctttttgaaa ttgtttccgg tatttcattg ggaacggcat aatgatattc attgattacc
     1861 ataccatttc ctctgtcccg gtaacgccag tggggtatgt caaataacag accttttttc
     1921 aatttgatat tgcagagcca gtatatcagg ggagaattgc tgaaggccct ttttattttg
     1981 tcgacaggca accggttatt aaaataaaag acaatgcgca tcacattact cccggaatga
     2041 tgaatacgtg catgcttgtc caacacccgg tggaagcaat ccgagccgga taacagcact
     2101 ttttcaggca ggattgattt atcgaagggt ctcattttta aaagcagtta aacggataat
     2161 ctatttacca gccgcgacaa agacgccagg gttttgaaat tttccggcag caactgatcg
     2221 tccggaacga gcatattata cttttcctga acaaagagca ggatttcaat catggaaaaa
     2281 gaatctattc cggcgttttg taaaattgtt tccgcatcca gttttatatt cccggccaga
     2341 atattgacct cgatgaattc ctttatatcg cctatgatga tattttcatc catgatttat
     2401 acctctcttt ttatatggtt tgtaaaggat gaaatagcca tcgttgcgat ggaaaagata
     2461 accagcttca ttgcgtcagc gagaatggcc caggcgcctg atcctcttaa aaataattta
     2521 tagtacccat ccaatgacca gttcaacgga gagaaggcgc tgatggaccg catagattcc
     2581 ggcatgacat aagtaggcac ccagataccg cccagtgcag acaacaaaag gatggacagc
     2641 gagcccatga tggcgccttg ctgctccgtg gaggccagtg tgcccaccaa tacaccgtaa
     2701 ccggtggcgg ccagcgccgt ggcgagcgta agaatggcaa tcccccagaa
//
LOCUS       DASYCU010000247         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_343650, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000247 DASYCU010000000
VERSION     DASYCU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343650"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGM24_04950"
     CDS             <1..372
                     /locus_tag="VGM24_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4154746.1"
                     /translation="QPASTDAGKIYFRIRRKDGQGNISFSIVKGINLEQGDQRPGIQI
                     YPNPVDRHVTVLFDERQTGAFRLELISTTGQIIQQKQVQMAGNSVASMELAGHPARGV
                     YLLRAQDLGGNRMFLTKVIVD"
     gene            414..1097
                     /locus_tag="VGM24_04955"
     CDS             414..1097
                     /locus_tag="VGM24_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HEY4154747.1"
                     /translation="MEYNTTRNHLIMREYGRHIQRMIEYLLTIEDREVRERNAQSVIE
                     LMGFLNPHLKNVEDFRHKLWDHLFLISDFKLEVSSPYPIPTRETLRPKSDVLPYPKRY
                     PKYSHLGKNLELIIQKALQEENADKKNGLANAIAYYMKLAYNNWHKETVHDDAIQGEL
                     TAITNGQLEFTNTPFVRSYRPTENRERSGNGYGDYRNKRNQKFQRNNNNRNNDRSNDR
                     GGKFKKKRY"
     gene            1098..2402
                     /gene="murA"
                     /locus_tag="VGM24_04960"
     CDS             1098..2402
                     /gene="murA"
                     /locus_tag="VGM24_04960"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY4154748.1"
                     /translation="MNAFEVAGGKQLKGEITPQGAKNEALQVISAVLLTKGKITISNI
                     PDILDVNMLIELLEEMGVKINRISRHVCSFQADDVNVDYLNSETYRIKSGRLRGSIML
                     AGPLLARFKKAFVPKPGGDKIGRRRLDTHIIGFEKLGARFSYDTGADYFRLEAPELIG
                     TYLLLEEPSVTGTANIIMAATLATGTTTIYNAACEPYIQQLCHLLNSMGARISGTGSN
                     LLEIEGVMELGGTDHRILPDMIEIGSFIGMAAMTQSDLLIKDVNLPNLGVIPDKFRQL
                     GIRLEIKGDDIHIPAQDLYEIQTYLDGSVLTIYDHPWPGLTPDLLSVMLVVATQAKGS
                     VLIHQKMFESRLFFVDKLLDMGAQIILCDPHRAAVIGLGRAHRLRGITMSSPDIRAGV
                     SLLIAAMSAEGKSVIQNIDQIDRGYQYIDERLNRLGASIKRV"
     gene            2431..3003
                     /gene="gmk"
                     /locus_tag="VGM24_04965"
     CDS             2431..3003
                     /gene="gmk"
                     /locus_tag="VGM24_04965"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY4154749.1"
                     /translation="MLIIITAPSGAGKTSITRYLLHHFPQLAFSVSAATRPQRSDEIN
                     GVDYYFMSLDEFHKKIQNDEFIEWEMVYEGKYYGTLKTELKRIKDNHKVPILDIDVKG
                     AIHVQQQYSERALSIFVQPPSIEELKRRLELRATESPEALQDRLNKAAYEISFRHHFD
                     KIITNDNLERACQEAGAIISTLFTEEGILR"
     gene            3046..>4062
                     /locus_tag="VGM24_04970"
     CDS             3046..>4062
                     /locus_tag="VGM24_04970"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY4154750.1"
                     /translation="MINTNTLVWIIIAWLMLIFFAGIEIAFANANRLTIELKKKQGLN
                     RSILLSRLLDQPSRFLSAILIGFNISLVIFGLLVGETLSPLWEFIIKKTVVPQAWVDI
                     FRLMFETFTSTLFILFFGELIPKAFFRAKSDSLLDFFATVSNFFYNLLYPVSLFFTGI
                     SEWILKYVFNVRIDEGKDLFAKADLGNYFQKTGEGDDAQHELNTELFEAALSLPKVKV
                     RECLIPRKEIEGIDVGLPVDEVRKKFVSSKLSKLVVYDGNIDHVQGYVHQLDLFKGPR
                     LIRDILLPIPAVPESMSATDLISKFSRDRKSIAWVVDEFGGTAGIVTMEDLLEEIFGE
                     IRDEY"
BASE COUNT         1086 a          994 c         1050 g          932 t
ORIGIN      
        1 cagcctgctt caacggatgc ggggaaaata tatttccgga tcaggcggaa agacggccag
       61 gggaatatca gcttttccat agtgaaaggg atcaacctgg agcagggcga ccagcggccg
      121 ggaattcaga tctatccaaa tccggtcgat cgccacgtga cggttctttt tgacgaacgg
      181 caaaccggag cattcagact ggaactcatc agcactacgg gtcagattat ccagcagaag
      241 caggttcaga tggcgggaaa cagcgtggcc agtatggaat tggcgggtca tccggccaga
      301 ggggtttacc tgctccgggc ccaggatctc ggcgggaacc ggatgttcct gaccaaggta
      361 atcgtggact gattattttt tcgtaagttt gtcagcttaa agctttacaa ataatggaat
      421 ataacactac gcgtaatcat ctgattatgc gggaatatgg acgccatata cagcggatga
      481 tcgaatatct gcttacgata gaggaccggg aagtgcggga aagaaatgcg cagtccgtga
      541 ttgaactcat gggattcctg aaccctcatt tgaaaaatgt ggaggatttc cgtcataaac
      601 tatgggatca tcttttcctc atatctgatt ttaaactgga ggtttcttct ccctatccca
      661 tccccacccg ggaaacgctc cggcccaaat ccgatgtgct tccctatccg aaaagatatc
      721 cgaaatactc tcatctcggt aagaacctgg agctgatcat tcagaaggcc ctgcaggaag
      781 aaaatgcaga taagaagaac ggtctcgcca acgcgattgc ctattacatg aagctcgcct
      841 acaataactg gcataaagag acggtgcacg acgatgccat ccagggagaa ctgacggcca
      901 ttaccaatgg gcagctggag tttaccaata ccccgtttgt cagaagctac cggccgaccg
      961 aaaaccggga aagaagcgga aacggatacg gggactaccg caataaaagg aatcagaaat
     1021 ttcagcgaaa caataacaac cggaacaacg accggagcaa cgaccgcgga ggcaaattca
     1081 aaaagaaaag atattgaatg aatgcctttg aagtagcggg cggtaaacag ctgaaaggag
     1141 agattactcc ccagggagca aaaaatgaag cgctccaggt gatcagcgct gtcctgctga
     1201 caaaagggaa aataaccatc tcgaatattc ccgatattct cgacgtcaac atgctcattg
     1261 aactgctgga agagatgggg gtaaagatca accgcatttc ccggcatgtg tgcagctttc
     1321 aggcagatga tgtaaatgtg gattatctca acagcgaaac ctaccggata aaaagcggca
     1381 ggctcagggg ctcgatcatg ctggcgggac ccttgctcgc ccggtttaaa aaagcttttg
     1441 ttccgaaacc cggcggtgat aagatcggaa gaaggagact ggatacccat atcatcggat
     1501 ttgagaaact gggggcccgt ttttcctatg acacgggggc tgattatttc cggctggagg
     1561 cacctgaatt gattgggacc tatcttttgc tggaagagcc ttccgttacc ggtacggcaa
     1621 atatcatcat ggcggctacg ctggcaacgg gaacgacaac gatttataat gccgcctgcg
     1681 agccctatat ccagcagctc tgccacctgc ttaacagcat gggcgcacgc atcagcggaa
     1741 ccggaagtaa tttgctggag atagaaggag tgatggagct gggcggaacg gatcaccgga
     1801 ttcttccgga catgatcgag atcggctctt ttatcggtat ggccgcgatg acccagagtg
     1861 acctgctgat taaggacgtc aatctcccca acctgggcgt tattccggat aaattcaggc
     1921 agctgggcat ccggctggag atcaaaggag atgatatcca tattccggca caggaccttt
     1981 acgagataca aacctacctt gacggctcgg tgcttacgat ttacgatcac ccctggccgg
     2041 ggctcacccc cgatctactc agtgtgatgc tggtggtggc cactcaggct aaaggaagcg
     2101 tgctcattca ccagaaaatg tttgaaagca ggttgttctt cgtggacaaa ctcctggata
     2161 tgggcgccca gatcattctc tgcgaccctc accgggctgc ggtgatcggc ctgggaaggg
     2221 ctcaccgcct gcgcggaata accatgagca gtccggatat ccgggccggc gtttccctgc
     2281 tgatcgcggc catgagtgcg gaaggaaaaa gcgtgatcca gaatattgat cagattgacc
     2341 ggggctacca gtatatcgac gaacgcctga accggctggg ggcatcgatt aaaagagtat
     2401 aagggttcaa cgaataaaaa ttcagttccg atgctgatca tcataacagc accatccggt
     2461 gcaggcaaaa cgtccattac ccgctacctg ctgcaccatt ttccccagct cgctttttct
     2521 gtttccgctg ccacccggcc gcaacgctcg gatgagataa acggcgtgga ttattatttt
     2581 atgagcctgg atgaatttca taaaaagatt cagaacgacg aattcatcga atgggagatg
     2641 gtatacgagg ggaaatatta cggcaccctg aaaacagaac tcaaacgcat caaggacaat
     2701 cataaagtgc ccatcctgga cattgatgtt aaaggagcca ttcatgtgca gcagcaatat
     2761 tcggaaaggg cattatccat cttcgtccag ccgccttcca tagaagagtt gaaacggcgc
     2821 ctggaattgc gtgccacgga atccccggag gccctgcagg accggttgaa caaggccgcc
     2881 tacgagattt ccttccgcca tcatttcgat aagatcatca ccaatgacaa cctcgaaaga
     2941 gcctgtcagg aagcgggggc catcatttca accctgttta ccgaagaggg aatcctccgg
     3001 taacgggtgg caattctggg tttttcctgc tatattcgta tttgcatgat caataccaat
     3061 accctggtct ggattatcat tgcctggctg atgcttattt tttttgccgg catcgaaatc
     3121 gcttttgcca atgcgaaccg cctgaccatt gagctgaaaa aaaagcaggg actgaaccgg
     3181 agcatcctgc tttcccggtt acttgaccag ccttcccggt tcctcagcgc catcctcatc
     3241 ggctttaata tttcactggt catttttggc ctgctggtcg gggaaaccct ttcgccttta
     3301 tgggaattca ttattaaaaa gaccgtcgtg ccgcaggcct gggtggatat tttccgctta
     3361 atgttcgaaa ctttcacgtc caccctgttc atcctctttt tcggagaatt gatccccaag
     3421 gccttctttc gtgcgaaaag cgacagcctc ctcgacttct ttgcaacggt cagcaatttc
     3481 ttttacaacc tgctttatcc agtctccctt ttttttacgg gaatctccga atggatcctg
     3541 aaatacgtat ttaatgtacg gatcgatgaa ggcaaggacc tgtttgcgaa agccgatctg
     3601 ggcaattatt ttcagaagac cggggaaggg gatgatgccc agcatgaatt aaatacggag
     3661 ttatttgaag ccgccctttc cctgcccaaa gtaaaagtgc gggagtgcct gatcccgaga
     3721 aaagaaatag aaggcataga cgtcggcctt ccggtggatg aggtacgcaa aaaattcgtc
     3781 agcagtaaac tgagcaagct ggtcgtatac gacgggaata ttgatcatgt acagggttat
     3841 gtgcatcagt tggacttgtt taagggcccc cggctgatcc gggatatcct gctgcccatt
     3901 ccagccgtac cggaaagcat gagcgcaaca gacctgatca gcaaattcag ccgggataga
     3961 aaaagcatcg cctgggtggt ggatgaattt ggcggcacgg ccggcatcgt gaccatggaa
     4021 gacctcctgg aagagatctt tggcgaaatc cgggatgaat at
//
LOCUS       DASYCU010000248         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4260389, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000248 DASYCU010000000
VERSION     DASYCU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4260389"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3451)
                     /locus_tag="VGM24_04975"
     CDS             complement(<1..>3451)
                     /locus_tag="VGM24_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEY4154751.1"
                     /translation="PQLLKGPSAGLPRVYDLAIELIAHSDGRVDLNSLTRFTSSYQEV
                     SELKLGELWAIPIMLRLALIENLRRLAALIAKDRISKNLADYWADQMTQTAEKDPKSL
                     ILVIADMARYGPPMESSFVAELTRRLVGKGSAFALPISWIEQRLSEQGMTSDELVHIE
                     NQKQATDQVSIANSIGSLRFLSSNNWRDFVEDISTVESIFRSDPDDIYPLMDFRTRDE
                     YRHKVEKIAKNSDHSESEVARMAMDMAKEGDPSQPDYQKAGHLGYYLIGKGVKKLEKA
                     TAVRFSYPERIERTCRKIPLLLYGGLSFLGALFFTGALVSEAYKNGFQNPWAIGIIGF
                     FTLIACSQLSITLINWVSTVIVRPLVLPRMDFSEGIPESFRTLVAVPSIFNDLKELED
                     LLDGLEVRFLANRDPNMHFALLTDFKDSETETRPGEDELLQEAALRIKALNERYGKEK
                     NEIFFLFHRPRRYNAKDRIWMGYERKRGKLAEMNALLRGHGKENFSLIVGEASLYEKV
                     KYVITLDTDTQLPRDAGWKIAGTMAHPMNHPYYSEKKQRVVDGYGILQPRVAVSLPAE
                     DGTVFALMHTNEPGIDPYTRAISDVYQDLFGEGSFIGKGIYDVDAFEKALKGRFPENR
                     ILSHDLLEGSYARCGLLSDVQLYEAYPKRYAVDMKRRHRWIRGDWQIASWLWPWVPGG
                     SRRIMKNPISALSKWKIFDNLRRSLVPICLLLMLLFGWLFSGSPMFWTISVLVILILP
                     SMVSLFWEIWRKPQDVHMLPHLVISFRYSVHHFTQQVLTLLTLPFEALLNADAIFKTN
                     WRILISHRKQLKWEPSNNLLQRENETILSSYISMWFPPVLAALVFGWMSYENTPGIEV
                     SLPFLIGWLLVPAITWKISLPYKGKAVELSDDQKKFLFKLSRKTWAFFETFVVAGDNW
                     LPPDNYQESPVDRIAHRTSPTNIGLSLLANLTAWDFGYIQTEILLERTSNTLHTMHRM
                     EQYASHFYNWYDTLTLEPLLPKYISTVDSGNLAGHLLTLKQGLLALGDSPVVNSRVFK
                     GIKDTVDVLIEKAGDDKPFQSFLTELKAADAKEWQTFLSVRDQLQKLLIGAEGFAPSQ
                     NSSTEAAWWTNALIKQIQDALSDLFEAVPWLLLNEFPGRFQSLENMLSRIMTLNELAG
                     LEVS"
BASE COUNT          812 a          929 c          872 g          838 t
ORIGIN      
        1 agaaacttcc agaccggcca gttcatttaa tgtcatgatc cgggagagca tgttctccag
       61 ggattgaaag cgcccgggaa attcattcag cagcagccag ggaaccgctt cgaacagatc
      121 actcaatgca tcctgtattt gttttatcag ggcattcgtc caccaggccg cttccgtgga
      181 tgaattctgc gacggagcaa acccttccgc accgatcagc aatttctgca gctggtcccg
      241 gacgctcaga aaggtctgcc attcctttgc gtcggcggcc ttcaactccg tgaggaatga
      301 ctgaaagggt ttatcatccc ccgctttttc aatcagcaca tcgacggtgt cttttattcc
      361 cttaaagacc cggctgttca cgaccggcga gtctcccaat gccaataagc cctgcttcag
      421 cgtgagcaga tggcccgcga ggttcccgct gtcgaccgtt gaaatatatt ttgggagaag
      481 gggttcgagg gtcagggtat cgtaccagtt gtaaaaatgg gacgcatact gctccatgcg
      541 atgcatggta tgcagggtat tcgaagttct ttccagtaag atttcagttt gaatatatcc
      601 gaaatcccag gcggtcaggt tggccagcag ggaaaggccg atattggtcg gcgaggtgcg
      661 gtgggcgatc cggtctaccg gactttcctg gtaattatcc ggcggaagcc agttatctcc
      721 agccacgaca aaggtttcaa aaaaggccca ggttttccgg gaaagtttga acaggaattt
      781 tttctgatca tctgatagtt caacagcttt ccccttatac gggagactga tcttccaggt
      841 aatggccggt acgagcagcc agccgatcag gaaaggaaga cttacttcaa tacccggcgt
      901 attctcgtaa ctcatccagc caaaaaccag cgcggccagc accggcggaa accacatgga
      961 aatataactg cttaagatcg tttcattctc ccgttgcagc agattattgg aaggctccca
     1021 tttcaactgc ttccggtggc tgatcaggat ccgccaattg gttttgaaaa tggcgtctgc
     1081 gttcagcagc gcttcaaaag gaagggtgag cagggtgagc acctgttggg taaaatgatg
     1141 tacggagtac cggaaactga tcaccaggtg cggaagcatg tgcacatctt gcggttttct
     1201 ccagatctcc cagaaaagac tgaccatgga cgggaggatc aggatgacca aaaccgaaat
     1261 ggtccagaac atgggtgaac cggaaaatag ccagccgaac agcagcatca gtaacaggca
     1321 aatcggaacc aggcttcggc ggagattgtc aaatattttc catttggata atgcggaaat
     1381 gggatttttc atgatccgtc tggatccgcc gggaacccat ggccagagcc agctcgctat
     1441 ctgccagtca ccgcggatcc agcgatgacg gcgtttcata tccaccgcat accgtttcgg
     1501 ataagcttca tagagctgga catcactgag caaaccgcag cgggcataac tcccttccag
     1561 caggtcatga ctcaatatcc ggttttccgg aaacctgcct ttcaatgcct tttcaaaagc
     1621 atccacgtcg taaatgcctt taccgataaa cgatccttct ccgaatagat cctggtaaac
     1681 atcggaaatc gcacgggtat agggatcaat gccgggctca ttggtgtgca tcagggcgaa
     1741 gacggtgccg tcttccgcgg gcagacttac cgcgacacgc ggttgcagga tcccatagcc
     1801 gtccaccacc cgctgtttct tttcgctgta atacggatga ttcatggggt gagccatcgt
     1861 gccggcgatc ttccatcccg catcgcgcgg cagctgggta tccgtatcca gggtgatcac
     1921 atatttcacc ttttcataca gactggcttc ccccacaatg agtgaaaaat tttccttccc
     1981 gtgtccccgc aggagcgcat tcatttcagc cagtttaccc cgcttgcgtt cgtaacccat
     2041 ccagatcctg tcttttgcat tatatctcct cggccggtga aacaaaaaaa agatttcgtt
     2101 tttttccttt ccgtaacgtt cattcaacgc ttttatccgc aaggcggctt cctgcagcag
     2161 ttcgtcctcc ccgggccgtg tttccgtttc ggaatcttta aaatccgtca gcagcgcaaa
     2221 atgcatattc ggatcccggt tggccaggaa ccgcacttcc agaccgtcca gcaggtcttc
     2281 cagttctttc agatcattga atatggaagg aaccgccacc agggtacgga aggattccgg
     2341 aattccttcg gaaaaatcca tgcggggtaa aaccagcggc cggacaatga ccgtcgatac
     2401 ccagttgatc agggtgatgg aaagctgact gcaggcgatc agggtgaaaa aaccaataat
     2461 gccgattgcc cagggatttt gaaaaccatt tttatacgcc tcgctgacga gggccccggt
     2521 aaaaaacaag gcgcccaaaa aactcagccc tccataaagg agcaggggaa tttttctgca
     2581 ggtcctttct attctttccg gataggaaaa gcgcacggcc gttgcttttt ccaacttctt
     2641 cacccccttt ccaatcaggt aatatcccag gtggccggct ttctgataat ccggctggga
     2701 cggatctcct tctttagcca tgtccatggc catgcgggcc acttccgatt cggaatggtc
     2761 gctgttcttg gcgatctttt ccaccttgtg ccggtattcg tcgcgggtgc ggaaatccat
     2821 caaaggatat atgtcgtcgg gatccgaacg aaaaatgctt tccaccgtgc tgatatcttc
     2881 tacgaaatcg cgccagttat tgctgctcaa aaaccggagg ctcccgatgc tgttcgcaat
     2941 agagacctgg tcggtggcct gtttctgatt ttctatgtga accagttcgt cgctggtcat
     3001 tccctgttcg gataatcgct gttcgatcca gctgatcggc agggcgaacg cagatccctt
     3061 ccccacgagc cgtcgcgtga gttcggcaac aaaagaactt tccattggcg gcccgtagcg
     3121 ggccatgtct gcaatcacca ggatcagact tttagggtcc ttctccgcgg tctgcgtcat
     3181 ctgatcggcc cagtaatccg ccaggttctt tgagatcctg tcctttgcaa tcagggcggc
     3241 cagcctgcgc agattttcaa tcagcgccag gcggagcatg atggggatgg cccagagttc
     3301 tcccagtttc aattcggaaa cttcctgata ggaagaggta aatctcgtga ggctgttcag
     3361 gtccacgcgg ccatcactgt gggcaatgag ctcaatggcc agatcgtaca cccgcggtaa
     3421 tcccgccgaa ggacctttca gcaactgggg c
//
LOCUS       DASYCU010000249         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3244488, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000249 DASYCU010000000
VERSION     DASYCU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244488"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VGM24_04980"
     CDS             <1..896
                     /locus_tag="VGM24_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEY4154752.1"
                     /translation="YPVRLAHEYWRISGDNSPFGSRWKEAIALIVKTFKEQQRKTDHG
                     PYHFERNTAWATDGVPLGGYGYPVKPVGLICSMFRPSDDATIFPFLVPSNFFAVVSLR
                     QAAEMLNAIGKEAGLANECEQLAGEVEKALKQYATTTHPGVGKVYAYEVNGFGSFNLM
                     DDANVPSLLSLPYLGVLPKTDPVYQNTRKLILSPENPFFFRGRAAEGIGGPHAGLDMI
                     WPLGLIIRALTSSQVAEIKQCLKWLIASNAGTGFMHESFHKDDPARFTRKWFAWANTL
                     FGELIMKLHEHNPALLNVIPQ"
     gene            complement(883..1536)
                     /locus_tag="VGM24_04985"
     CDS             complement(883..1536)
                     /locus_tag="VGM24_04985"
                     /inference="COORDINATES: protein motif:HMM:NF018766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEY4154753.1"
                     /translation="MKTRFTGMERILILSVCFSISLLVLRILYTKEYAYAFYIWNTFL
                     AIIPFLCSRKLGSQNRMGLKSILLIGGWLLFFPNAPYLVTDLFHFTVRTGTPAWFDMI
                     LVSSSSWNGLALGIFSLLQVEQFLNRHFKEIRVRFISYAFILLCGYGIYLGRFLRFNS
                     WDIVTNPRPLFSSVYHSVLHPVQHTGTWAFTVTFSCLLGIVYTTIKQMQKRTGIIEG"
     gene            complement(1533..>1845)
                     /locus_tag="VGM24_04990"
     CDS             complement(1533..>1845)
                     /locus_tag="VGM24_04990"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY4154754.1"
                     /translation="GYFFRHEKNGRIQSVIGLCTLGLAAFFNITPVEWVAIIFCIGAV
                     LSLEMLNSALEKLCDLVQPDFHPRIKIVKDVAAGAVLWASIISAVTGLIIFLPEIVKY
                     L"
BASE COUNT          501 a          446 c          482 g          416 t
ORIGIN      
        1 gttatccggt ccggctggcc catgaatact ggagaataag cggggacaat tctccattcg
       61 gcagccggtg gaaggaagca atcgccttaa tcgtaaaaac atttaaagag cagcagcgca
      121 aaacagatca cgggccttat cattttgaac gaaatacggc ttgggcaacg gacggggtgc
      181 ccttgggcgg ttatggctat ccggtgaagc cggtcggact gatctgctcc atgttccggc
      241 ccagtgatga tgcgaccata ttccccttcc tggtcccttc caattttttt gccgtcgttt
      301 cactccggca ggcagcggag atgctgaacg ccatcggcaa agaagccggt ctggccaatg
      361 aatgcgaaca actggccggc gaagtggaga aggcgttgaa gcaatatgcg accaccacgc
      421 atccgggagt tggaaaagta tatgcgtacg aggtgaatgg tttcggcagt ttcaatttaa
      481 tggatgatgc caatgtgcct tcccttcttt ccttacctta tctgggcgta ttgccaaaaa
      541 cggatccggt atatcagaac acccgaaaac tgattttgtc tccggagaac cccttctttt
      601 tcaggggcag ggcggcagaa ggcatcggcg gccctcatgc cggtttggat atgatctggc
      661 cgctgggcct tattatccgg gcattgacga gctctcaggt cgcggagata aaacaatgtc
      721 tgaaatggct gatcgcttcc aatgcaggaa ccggcttcat gcatgagtcc tttcataaag
      781 atgatcccgc caggtttacg cgcaaatggt ttgcctgggc gaataccctt ttcggcgaac
      841 tgatcatgaa attgcatgaa cacaacccgg ctttgctgaa tgtcatccct caatgatccc
      901 cgtccgcttt tgcatttgtt ttatggtggt ataaacgatt cccagcaggc agctgaaggt
      961 aaccgtgaat gcccaggtgc cggtatgttg taccgggtga agaactgaat gatatacgga
     1021 ggaaaaaagc ggccgggggt tggtcacgat atcccagctg ttgaaacgca ggaagcgccc
     1081 caggtaaatg ccatatccgc aaagcagaat aaaggcatag gaaataaacc ggacccggat
     1141 ctccttaaaa tgcctgttca ggaattgttc cacctgcagc agggaaaata tgcccagggc
     1201 cagtccgttc caggaagagg aactgaccag gatcatatcg aaccaggccg gcgttccggt
     1261 tcttaccgta aaatgaaaaa gatcggtaac caggtagggg gcgttgggaa agaatagcag
     1321 ccacccgccg atcagcagta tggattttaa ccccatccgg ttctgggaac ccagctttct
     1381 gctgcagaga aagggtatga tcgcgagaaa ggtattccag atataaaaag cataggcata
     1441 ttccttcgta tataaaatgc gaagaaccag gagggaaatc gaaaaacaaa cggagaggat
     1501 taaaatccgc tccatgccgg tgaatcgtgt tttcatagat attttactat ttcaggtaaa
     1561 aaaatgatca gccccgtgac cgcactgatg atggaagccc agagaacggc ccctgcagcc
     1621 acatctttta caatttttat ccggggatgg aaatccggct gcaccaggtc acataatttt
     1681 tccagcgcgc tgttgagcat ttccagagac aaaaccgctc caatgcaaaa gataatggcc
     1741 acccattcga cgggcgtaat gttaaaaaaa gccgccaggc ccagggtgca aagaccaatg
     1801 acagactgaa tccggccgtt tttttcgtgc cggaaaaaat atccg
//
LOCUS       DASYCU010000250         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2229577, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000250 DASYCU010000000
VERSION     DASYCU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229577"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1406
                     /locus_tag="VGM24_04995"
     CDS             252..1406
                     /locus_tag="VGM24_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY4154755.1"
                     /translation="MPFYIQRGQIPPKRHTQFRQPDGTLYAEQLFSTEGFSNDSSLLY
                     HCHPPTAIIHIDDPVPVMPEIVEEKMLKHRSLEGFNIKPVPDFLDSRTPVLVNNDLHI
                     SLAAPGESMKDYFYKNADADELIFVHEGNGRLESQYGSVPFWEGDYLVIPRGTIYQIH
                     FNDPRNRLFLVESFSPIRFPDKYLSRYGQLLEHSPYCERDIHGPGEGKPVNEKGDFLI
                     KTKKRGILYGIHYGHHPFDVVGWDGCCYPFTLSIHDFEPITGRVHQPPPVHQTFEGRN
                     FVVCSFVPRLFDYHPLSVPVPYNHSNIDSDELLYYVDGDFMSRRQVTRGMITLHPAGI
                     PHGPHPGTVEKSLGAKETKELAVMLDSFHPLMLTRAALDIENKDYINSWT"
     gene            1403..>1920
                     /locus_tag="VGM24_05000"
     CDS             1403..>1920
                     /locus_tag="VGM24_05000"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY4154756.1"
                     /translation="MKINRDILTLEEFRKQELRSLPNATGELSNLLRDIALAAKRVNV
                     EVNKAGLVDILGETGSVNIQGESVQRLDEFANTEFMAVLQRGVSCAGIASEELDEFVA
                     FNEELCNQSKYVVVMDPLDGSSNIDVNISIGTIFGVYRRKSLKGQACALPDFLQKGEN
                     LVAAGYIIYGSST"
BASE COUNT          491 a          464 c          473 g          492 t
ORIGIN      
        1 ttttcgtcga ggccagtgat gttgcccgtt aactgcatct gttgatctgt ttatgttcat
       61 gcaaataatg cattcatagg aatggatcac agatcgcatg ggcccgttaa gtcattagcc
      121 gcttataatg cttataagga gtgtgcgtga aagaaatttc agaggaatgg ggtgtgtgcc
      181 ataaaaataa aaatatttta acgaaaacaa aatatttttt ggatcggcgt accttgattt
      241 aaatatttct tatgcctttc tacattcagc ggggccagat tccacccaaa cggcataccc
      301 aattccgcca gccggacgga actctgtatg cagaacagct gttctccaca gaaggctttt
      361 ccaacgattc ctccctgctt taccattgcc atccccccac cgcgatcatc catattgacg
      421 acccggttcc ggttatgccc gaaatcgttg aggaaaaaat gctgaaacac aggagcctgg
      481 agggttttaa cataaaaccg gttcccgatt ttctggacag ccgtacgccg gttctggtca
      541 ataacgacct gcatatttcc ctggccgccc ccggggaatc catgaaggac tatttttaca
      601 agaatgccga cgcggatgaa ttgatttttg ttcatgaagg aaacggaagg ctggaaagcc
      661 agtatggatc tgtcccattt tgggaaggcg attacctggt cattccgagg ggtacgattt
      721 atcagatcca ttttaatgac cctcgtaacc gccttttcct ggtagagtct ttcagtccca
      781 tccgcttccc ggacaaatat ctgagccggt atggccagtt actggaacat tctccttatt
      841 gcgaacggga tattcatggg cccggcgaag ggaaaccggt aaatgaaaaa ggagatttcc
      901 ttataaaaac caaaaagcgg ggcatccttt acgggattca ttacgggcac cacccgtttg
      961 acgtagtggg ctgggatggc tgttgttatc cgtttacgct atccattcac gattttgaac
     1021 ccatcaccgg ccgggtacac cagcctcctc cggttcacca gaccttcgaa ggccggaatt
     1081 ttgtggtttg ttcttttgtt ccgcgtttat ttgattacca tcccctgtct gttccggttc
     1141 cctataatca cagcaatatc gacagcgacg aacttcttta ttatgttgac ggtgatttca
     1201 tgagccgccg gcaggtaacg cggggcatga tcaccctgca ccctgccggg attccgcacg
     1261 gaccgcatcc cggaaccgtg gagaaaagcc tgggtgctaa agaaaccaaa gagctggccg
     1321 ttatgctcga cagctttcat cctttaatgc tgacccgggc ggccctggac atcgaaaaca
     1381 aagattatat caattcatgg acatgaaaat taaccgggac atcctcaccc tggaagaatt
     1441 cagaaaacag gaattgagat cattgccaaa cgccaccggc gaattaagca acctgctgcg
     1501 cgatattgcg ctggccgcca aacgggtgaa tgtggaagtg aacaaagccg ggctggtgga
     1561 cattctcggg gaaaccggct ccgttaatat tcagggggaa tccgtgcaaa ggctcgacga
     1621 gtttgccaat acggagttta tggccgtatt acagcggggc gtaagttgcg ccggaattgc
     1681 atccgaagaa ctggatgagt tcgtcgcctt caatgaagag ctctgtaacc agtccaaata
     1741 cgtggtggtg atggatccgc tggatggcag cagcaatatc gatgtgaaca tttccattgg
     1801 taccatcttt ggcgtttacc ggagaaagag cttaaaaggt caggcctgcg cattgccgga
     1861 ttttttgcag aaaggggaaa acctggtcgc tgccggatat attatttacg gatcttccac
//
LOCUS       DASYCU010000251         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3681083, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000251 DASYCU010000000
VERSION     DASYCU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3681083"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VGM24_05005"
     CDS             complement(<1..326)
                     /locus_tag="VGM24_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154757.1"
                     /translation="MNGFFHTLRIEWMKVKNYRTFWILTGIIVVCIPAFNYVIFDVTN
                     NTFPKINGQSILGSPFSFPNVWKTVPYNAGLLLFIPAILIITLITNEFTYRTHRQNII
                     DGWSRGR"
     gene            complement(329..1231)
                     /locus_tag="VGM24_05010"
     CDS             complement(329..1231)
                     /locus_tag="VGM24_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4154758.1"
                     /translation="MASILSLKNISKSYGKIQALRSVSFDVPPGAVFGILGPNGSGKT
                     TLLGVVMDVLKPSGGSFTWFEKPPSDRQRREIGSLLETPNFYHYLSGQRNLQIAASIK
                     EKPRQDIERVIKLVRLYERRNSKFQTYSLGMKQRLAIASCLLGNPSVLVFDEPTNGLD
                     PSGIAEIRELIIELSKEGKTIILASHLLSEVEKVCTHVAILQQGSLITSGSVKTVLSN
                     EEWLELRAAPMEKLKKILNQFPGITKIEESDNNLLAFFDQPMPDAGSLNQYCFEQGVV
                     LNHLQLRKKSLESEFIALTNNVSN"
     gene            complement(1290..1949)
                     /locus_tag="VGM24_05015"
     CDS             complement(1290..1949)
                     /locus_tag="VGM24_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M10"
                     /protein_id="HEY4154759.1"
                     /translation="MGEAELHKDSSKLIIHSDFYFYGDAASAEMAAAIAADIANHWNE
                     PRGTARHRRTLLAVEFVINGFYVPGLLPETVWYNTDPRNNYFRIEEFSLAHVSFVDAI
                     NCNTGYFKLDNLLNNSTTAAHEYGHTIGLDHPQTLDIRGKGQPGIMYPRGTVCDPGFQ
                     YDPEAEPLKAGGTLNPFNRKVLQQDIDLLKLDKLNLNREGIAVLGGFSSIYHEKHSAP
                     Q"
     gene            complement(1979..3118)
                     /locus_tag="VGM24_05020"
     CDS             complement(1979..3118)
                     /locus_tag="VGM24_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY4154760.1"
                     /translation="MQKVIIAFIGLACLSLAACHSSESGKGKHAPKDSSYVILGKITG
                     QDTGTVYLQHRDFSETIDSTKLDHSYFTFKGKADSVEYCYLGILAEGKKQFRTSFFLQ
                     NGKMTVLAKKDSLENALISGAPVQDEYNDYQNAIEKGIGIRSAALDKKYSVALAKGDK
                     KTTDSLEKEYDALDMEQKKMVSGYVKAHPASYISAFEIAQNFSYNPDPDQLDSLYKNL
                     DPAIQSTYFGRMVNNTLKKARLTAIGQPAPDFTSTATDGKEVSLSSFRGRYVLVDFWA
                     SWCGPCRRENPAVVKAYHQFHPKGFDILGVSLDNSKTSWEEAIKKDGLNWAQVSDLRG
                     WKNDVASRYGIQGIPMNFLLDKEGRIIAKGLRGEDLTKKLAEVIH"
     gene            complement(3128..>4187)
                     /locus_tag="VGM24_05025"
     CDS             complement(3128..>4187)
                     /locus_tag="VGM24_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4154761.1"
                     /translation="RLCYSPVKKEYVSAVPGDSSAFIVMKNFEDQTVWKNSAARLYHL
                     GDDVLGLEWYTKLGTIGGEVLEAIQRSVGIAEEKYKGLVIANEGQNFSAGANVGMIFM
                     LAVEQEFDELDMAIRLFQNTIMRTRYSSIPVVVAPHGLSLGGACELSLHADRVIPAAE
                     SYIGLVEAGVGLIPGGGGSKEFTLRASDELHDDEPETITLKNRFFTIATAKVSTSAFE
                     AFDLGILKKGRDLISINPDRRIADAKAAVLELYKDGYVAPTQRSDVKVLGRSALGALY
                     AGINGMRRANYATDHDVLVAKKLAFVMCGGDLSEPARVSEQYLLDLEREAFLSLCGEK
                     KTLERIQSVLKTGKPVRN"
BASE COUNT          996 a         1071 c         1023 g         1097 t
ORIGIN      
        1 cggcccctgc tccatccgtc aatgatattc tggcgatggg tccggtacgt aaattcattc
       61 gttatcagcg tgatgattaa aatagcggga ataaagagca gcagcccggc gttgtaaggg
      121 accgttttcc agacattcgg aaaggaaaag ggactgccca gaatgctttg tccgtttatt
      181 ttgggaaagg tattgttcgt gacatcgaag atcacataat taaaagcagg tatgcaaacg
      241 acaataatgc ccgtcaggat ccagaaagtg cggtaattct ttactttcat ccattcgatg
      301 cgcagggtat ggaaaaatcc gttcatggct aattgcttac gttattggtg agcgcgatga
      361 attcggattc caggcttttt ttccggagct ggagatggtt gagcaccacg ccctgttcaa
      421 agcaatattg atttaacgat ccggcatccg gcatgggctg gtcaaagaat gccagcagat
      481 tattgtccga ttcctctatt ttggtgatcc cgggaaattg attcagtatc tttttcagct
      541 tttccattgg agcggcacgg agttcaagcc attcttcatt gctcaaaacg gtcttcacgc
      601 ttcccgaggt gatcaggcta ccctgctgga gtatggccac atgagtgcaa actttttcca
      661 cttccgataa tagatgactg gccaggatga tggttttccc ttctttcgag agttcaatga
      721 tcagttcccg gatctctgct atgccggacg gatcgagtcc gttcgtgggt tcatcaaata
      781 ccagcacgga gggattgccc agcaggcagg aggcaatcgc aagccgctgt ttcattccga
      841 gggaataggt ctggaatttt gaatttcttc tttcatacag ccggaccagt ttgatgaccc
      901 gttctatgtc ctgccggggt ttttctttaa tggaagcggc aatctggaga ttccgctggc
      961 cggagaggta atgatagaaa ttaggcgttt ccagcaaact gccgatctcc cttctttgtc
     1021 tgtccgatgg aggtttttca aaccaggtaa aacttccgcc actgggtttg agtacatcca
     1081 tgacgacccc cagcagcgtg gtctttccgc tgccgttcgg accaaggatt ccaaaaacgg
     1141 ctcccggcgg cacgtcaaaa gacacggacc ggagtgcctg gatctttcca taggatttgc
     1201 tgatattctt tagggacaaa atggaagcca tagcgatata atcaggaaat ggttcactaa
     1261 agtaaggaat ataatgactt tgatacgatc tattgaggag ctgagtgttt ctcatgatag
     1321 atgctggaaa aacctccgag aacggcaatg ccttcccggt tcaagttcag tttatccagt
     1381 ttcagcagat caatatcctg ttgtaatact ttacggttaa acggattgag cgtacccccg
     1441 gccttaagcg gttccgcctc cggatcatat tgaaagcccg ggtcacatac cgttccccga
     1501 ggatacatga tgcccggttg ccctttaccc cggatatcca gcgtttgcgg atgatcgaga
     1561 ccgatcgtgt gcccgtattc atgcgccgcc gtagtggaat tgttcagcag attgtccagc
     1621 ttgaaataac cggtattgca attaatggcg tccacaaaag agacatgcgc cagggagaat
     1681 tcctcaatgc ggaaataatt atttctgggg tccgtattgt accagaccgt ttcgggcagg
     1741 agacccggaa cataaaatcc gttgatcacg aactccacgg ctaaaagcgt cctccggtgg
     1801 cgggcggtgc cccgcggttc attccagtga ttggcgatgt cggcggcaat ggcagcggcc
     1861 atttccgcgg aagcggcatc cccataaaaa taaaaatcag aatgaatgat cagcttgctg
     1921 gaatccttat gcaattcggc ttcacccata aaatggtatc ataaagaacc ggatccttct
     1981 aatgaatcac ttctgccaac ttttttgtca ggtcctctcc gcgcagtccc ttggcgatga
     2041 tccgcccttc cttgtccagc agaaagttca tgggaattcc ctgaatgccg taccgggaag
     2101 ctacatcgtt cttccatccc cggagatcgg atacctgcgc ccagttaagg ccgtcttttt
     2161 taatggcttc ctcccaggac gtttttgaat tatccagcga tacgcccagg atgtcaaatc
     2221 ctttgggatg aaactggtga taggccttaa ccacagccgg gttctcccgg cggcagggcc
     2281 cgcaccagct agcccagaaa tccaccagca catatctgcc ccggaaggaa gagagactaa
     2341 cctcctttcc gtccgtagcg gttgatgtga aatccggggc aggttggcca atagcggtaa
     2401 gcctggcttt tttcagcgtg ttatttacca ttcttccgaa atacgtggat tgaatggctg
     2461 gatccaggtt tttgtagagg ctgtccagtt gatccggatc cggattgtat gaaaaattct
     2521 gagcgatttc gaaggcagaa atataggatg ccggatgcgc ttttacataa ccggacacca
     2581 ttttcttttg ttccatatcc agggcatcat attctttttc caggctgtcc gtggtttttt
     2641 tatccccctt tgccagggca accgaatatt tcttatccag cgccgcggaa cggatcccga
     2701 tccctttttc aatggcattt tgatagtcat tgtattcatc ctgcaccgga gccccggaga
     2761 tcagggcatt ctccagggag tcctttttgg cgaggaccgt cattttacca ttctgcagaa
     2821 aaaaggaggt ccggaactgc ttcttaccct ccgccaggat tcccaaatag caatactcga
     2881 cgctgtcggc cttgccttta aaggtaaaat aactatgatc cagctttgtg gaatcgatgg
     2941 tctcggagaa gtcccggtgc tgaaggtaaa ccgtaccggt atcctgaccg gtaatttttc
     3001 cgaggataac ataggagctg tctttcggag catgttttcc cttacctgat tcggaggaat
     3061 gacaggcagc aagggaaagg catgcaagtc cgataaaggc aataatgact ttttgcatat
     3121 gatcaattta atttcttacc ggtttcccgg ttttcaacac gctttgtatt cgttccagcg
     3181 ttttcttctc cccgcaaagg gagagaaagg cttccctttc caggtcaagc aggtattgtt
     3241 cggacacccg cgccggttca ctcaggtctc ccccgcacat gacaaacgcc agtttttttg
     3301 ccaccagcac atcgtgatcg gttgcatagt ttgcccgccg catgccattg atgcccgcat
     3361 atagagcccc cagcgcggat ctccccaata ccttcacgtc gctccgctgg gtgggcgcca
     3421 cataaccatc tttatacaat tcgagcaccg cggcttttgc atccgcgatc cgcctgtcgg
     3481 ggtttatgga aataagatct ctcccctttt tcagaatgcc cagatcgaag gcctcaaagg
     3541 ctgaagtgga aacttttgcc gtagcaatag taaaaaaccg gtttttcagc gtgatggttt
     3601 ccggctcgtc atcatggagt tcatcggaag cgcgcagggt gaattccttg gaaccgcccc
     3661 caccggggat aagtcctact cccgcttcca ccaatccgat ataggattca gccgcgggaa
     3721 ttacccggtc tgcatgcaga ctcaattcac aggcgccacc caggctaagg ccatgcggcg
     3781 caaccaccac cggaatggaa gagtagcggg tgcgcatgat agtattctga aacaggcgga
     3841 tggccatatc cagttcatca aattcctgct ccacggcgag catgaagatc atacccacat
     3901 tggcgccggc agaaaaattc tggccttcgt tggcaatcac gagtcctttg tatttttcct
     3961 cagcgatccc gacggagcgc tggatggctt ccagcacttc ccctccgatg gttccgagtt
     4021 tggtatacca ttccaggccc agcacgtcat cgccgagatg ataaaggcgg gcagcgctgt
     4081 ttttccaaac cgtctgatcc tcaaaattct tcattacgat gaacgcgctg ctatctccgg
     4141 gaacggcaga aacatattct tttttgacgg gagaataaca gagcctt
//
LOCUS       DASYCU010000252         4763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1797440, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000252 DASYCU010000000
VERSION     DASYCU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797440"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VGM24_05030"
     CDS             <1..993
                     /locus_tag="VGM24_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HEY4154762.1"
                     /translation="ALIRARIPYLPVHADHIDREAAGLSVLILPNLALMTADQVSSVK
                     RFVEKGGSLLATGDSSLFDEWGDPLPDFALAGLFGAHLLKPRNPDMKKENKMAGDSYH
                     TYLRLSPELRSRVDGPHNGNEPPITGERHEILEGFGETDILPFGGLLDPLRLDTGSKV
                     LFSFIPQFPVYPPEKAWMRIPRTDIPGLILNTRENGARIVFLPADIDRQYGRSNLPDH
                     GNLLKNIVRWAAKEDLPLSVEGAGLVDSHLYRQADRLILHLVNLTNENTWRQPLDELI
                     SIGPLRVNVKLPSGIKGTSLRLLVSGKTVNAEVRNNWCQFEINSITDHEVIVIY"
     gene            complement(1016..3646)
                     /locus_tag="VGM24_05035"
     CDS             complement(1016..3646)
                     /locus_tag="VGM24_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY4154763.1"
                     /translation="MYKRLFIAAHFLCLVLLVSAQDRYELNAGWKCINAGKLKAGGEE
                     ISLPGFAIGDWIPATVPGTVLTTLINNELMPDPFYGMNNEKIPDIYTTGPEYYTYWFA
                     KDFKEAAPKGDQQVWLHFRGVNYSCDVYLNGHQLNGKRHRGMFLRQTYNISAFLSKTG
                     LNRLAVIVYPPDPVGNPNGGQGGDGEIARSITHQYVAGWDWIQPVRDRNTGIWDKVEI
                     EKTGPVDISNPHLITLVPGTRDPDAKFQAPAILRVSAELENASKQIVRGSLKFKVRGT
                     VVSRTVSLEPGSTREVNFPDFTLHNPALWWPNGYGQQSLYKAEIYFERDAKTVSDRES
                     LPFGVREIRTAWNSVTHSREVRVNGQPVFIKGGNWIVSDELLRFSPERYDAEVRFHRD
                     MNLNLIRVWGGALTERPEFYQACDKYGILVFQDFWNSGDCNGRWTDPRKKEDQWTRRQ
                     YPDDHALFLASVRDQVKMIRNHPSLAFWCGGNEITPPDDILAAMKDSILPALDGTRYF
                     FDYSNSDSMSFNSLGGNGDGPYGLQNIRTFWAERSFPFNSEVGSVGLGDYESLERFLP
                     PENLQVAPQYPGKTDPVWAYHKFSRYDSTIYAYGPVKDLKDFTLKAQLVNYDQYRALM
                     EGFSSHMWEWYTGFIIWKTQNPWTAMRGQMYDYYLDVNAGLYGLHHGSEPLHVMFDPV
                     TNMVMIVNNTFRFYRNMMLEVRTYDLKGNTNLLTQVYEEIGPSAVRKYVNIGNGINKL
                     RKKEGIFLSLRLLDKDKQPINENFYWLPDSAGEYSGLQYMAKTEISVEARKTGKGKIT
                     VSLAEAAGNPVAFFNRIALIDPQTKKRILPVFYSDNYVSVVPGGSRTIMLEYPDTAGV
                     APPLVSVKGWNVEETTTVVK"
     gene            3855..>4763
                     /locus_tag="VGM24_05040"
     CDS             3855..>4763
                     /locus_tag="VGM24_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HEY4154764.1"
                     /translation="MNKKIYYRFLTAAAFGISACHSPAGEANEPAPPFNRDSLVSHII
                     ILASDSFQGRKPFTAGEEKTIAYLQQQFSSMGLEPGNGRSFLQDVPLVDISRTTDPVM
                     KVESARGSFPLKDGKDYVLSTESTDPVISLDKADLVFAGYGVVAPEYHWNDYAGIYVK
                     GKVVLVMVNDPGFGTTDTSIFKGRTMTYYGRWTYKFEEAARQGAKGCLIIHNTAAASY
                     PFSVVQSSWGSSSLFLDERKNPVYHCPFEGWVSADAAKKLLAAAGKDSSLLVQANKPG
                     FKAVPLEEKLSTSMRVKAVYNNSKNVI"
BASE COUNT         1153 a         1265 c         1177 g         1168 t
ORIGIN      
        1 gccctcatcc gggcgcggat accttacctt cccgttcacg cggatcatat tgaccgggag
       61 gcggccggcc tttctgtgct gatccttccc aacctggccc tgatgaccgc ggatcaggtt
      121 tcctccgtaa aacgctttgt ggaaaaaggc ggcagcctgc ttgccaccgg cgacagcagt
      181 cttttcgacg aatggggaga tccgcttccc gattttgcgc tggccggtct ttttggcgcc
      241 catcttctca aaccgcggaa tccggatatg aagaaggaaa ataaaatggc tggagactcc
      301 tatcatacct atctccgcct tagccccgaa ctccgatccc gggtggacgg gcctcataac
      361 ggtaacgagc ctcccataac cggtgaacga catgagatat tggaagggtt tggggaaacg
      421 gatatccttc cgttcggcgg tctgctcgat ccgcttcgcc tggacaccgg ttccaaagtg
      481 ctgttcagct ttattccgca atttccggtc tatcctccgg aaaaagcctg gatgcggata
      541 ccccggaccg atatacccgg actgattctc aacacccggg aaaacggagc ccgcatcgtt
      601 ttcctgccgg ccgatatcga ccggcaatac ggccggtcca atctcccgga tcatggaaac
      661 ctgctgaaga acatcgtacg gtgggcggca aaagaagatc ttccgttgtc cgttgaaggg
      721 gccgggctgg ttgacagcca tctctaccgg caggcagacc ggttgattct tcacctggtt
      781 aacctgacca atgaaaacac ctggcgccaa ccactggatg aactgatttc catcggtccg
      841 ctccgggtaa atgtgaaatt gccttccgga ataaaaggaa ccagcctgcg tttgctggta
      901 tcgggaaaga ccgttaatgc ggaggtcagg aacaactggt gccagtttga aatcaattcc
      961 ataacggatc atgaagtgat cgttatctac tgaatatccg gcaggccgta tcgatctatt
     1021 taaccaccgt ggttgtttct tccacattcc atccttttac ggaaactaaa ggcggcgcaa
     1081 ctcctgccgt atccggatat tccagcataa tcgtccggct gccgccggga accacggata
     1141 cataattatc actgtaaaaa acgggcagga tccttttttt agtctgcgga tcgatcagag
     1201 cgatgcgatt aaagaaagcc accgggttgc cggctgcttc agcgagcgat acggtaatct
     1261 tgccctttcc ggtctttctg gcttccacgc ttatttctgt tttcgccata tactgaaggc
     1321 cggaatattc accggcggag tcgggcaacc aatagaaatt ctcattgatg ggttgtttgt
     1381 ctttatccag caggcgaagg gataaaaata taccctcctt ctttctcaat ttattgatgc
     1441 catttccgat attaacatat tttctaaccg cagaaggccc gatctcctca taaacctgcg
     1501 ttagcaaatt tgtatttccc ttcaggtcat acgttctcac ctccagcatc atattgcggt
     1561 agaatctgaa cgtattgttt acgatcatca ccatattcgt aacaggatca aacataacgt
     1621 gcaggggttc gctgccgtgg tgaagtccgt acaggcccgc gttcacgtcc aggtaataat
     1681 catacatctg tccgcgcatg gccgtccatg ggttctgcgt cttccatata ataaagccgg
     1741 tataccattc ccacatgtgc gaactgaatc cttccatcaa tgcccggtac tgatcataat
     1801 tcactaattg cgccttcagc gtaaaatcct ttaaatcttt aaccggtccg taggcataga
     1861 tcgtggaatc gtaccggctg aatttatgat aggcccagac cggatccgtt ttgcccggat
     1921 attggggcgc aacctgaagg ttttccggag gaagaaagcg ttccagcgat tcataatccc
     1981 cgagacccac ggatcccact tctgaattaa agggaaagct ccgttcggcc cagaacgtgc
     2041 ggatgttctg caacccgtag gggccgtccc cattaccacc cagggaattg aaggacatgc
     2101 tgtctgaatt cgaatagtcg aaaaaatacc gtgtaccatc cagggcgggc agaatagaat
     2161 ccttcatcgc cgcgagaata tcatcgggcg gcgtgatttc gttgccaccg caccagaaag
     2221 ccagggaagg atgattccgg atcatcttca cctgatccct gacggatgcc aggaacaggg
     2281 cgtgatcatc cgggtattgc ctgcgtgtcc attgatcttc ctttttcctg ggatcggtcc
     2341 accgcccgtt gcagtcgccg gaattccaga aatcctggaa aaccaggatc ccgtatttat
     2401 cacaggcctg gtagaattcc ggcctttccg ttaaagcgcc tccccagacg cggatgaggt
     2461 tcaggttcat atcgcggtga aaccggactt ccgcatcata gcgttccgga ctgaagcgaa
     2521 ggagttcgtc ggaaacaatc cagttgccac ctttgataaa taccggttgc ccgttcaccc
     2581 ggacctcccg gctatgggta accgaattcc aggcggtacg gatctcccgc acgccgaaag
     2641 gcagcgactc ccggtcagaa actgttttag catcgcgctc aaaataaatt tcagccttat
     2701 aaagggactg ctggccataa ccattaggcc accagagtgc cggattatgt agagtgaaat
     2761 ccggaaaatt cacctcgcgg gttgagcccg gctccaggct gacggtccgg gaaaccactg
     2821 ttcctcttac tttaaatttc aatgacccgc ggacaatttg tttggacgca ttttcaagct
     2881 ccgcgctgac cctcaggatc gcgggtgcct gaaattttgc gtccggatcg cgggtgcccg
     2941 gtacgagggt aatgaggtgc ggattggaaa tatcgaccgg gccggttttt tcgatttcca
     3001 ctttatccca tatgccggta ttgcggtccc tgacgggttg gatccagtcc caaccggcaa
     3061 catactgatg cgtgatgctt ctggcaattt ctccgtcgcc cccctgcccg ccattgggat
     3121 tccccacggg atccggcgga tacacaatta ccgccaggcg gttaagacct gtttttgaaa
     3181 gaaaagcact gatattatag gtctgccgga ggaacatgcc ccgatgtctt tttccgttca
     3241 attggtgccc attcaggtaa acatcgcaac tataattcac cccccggaaa tgaagccaaa
     3301 cctgctggtc cccttttggg gcagcttctt taaaatcttt cgcgaaccaa tacgtataat
     3361 attccggtcc ggtagtatag atatccggga tcttttcatt attcattccg taaaaaggat
     3421 cgggcatcag ctcattgttg atcagggtcg tcagcacggt gccgggcacc gtagcgggaa
     3481 tccagtcgcc gatggcaaag cccgggagag agatctcttc tccgccggct ttcagcttgc
     3541 cggcattgat gcatttccat ccggcgttga gctcataccg gtcctgcgcg gagacaagaa
     3601 gaactaaaca aaggaaatgg gcggcgatga atagtctttt atacatttat tatccggatt
     3661 ttcttaagaa gcgtttttcg tatgcgctga aattcccgta ctaaattata cattatacac
     3721 taagaacctc cggtcgccct gaatttaaag aagttaatcc ggcccggcgt ttccgcaaga
     3781 ccatacccca tctgataaaa caggcagaat acttatcttc gaagaattta ttttattcat
     3841 ccggaatttt tatcatgaac aaaaagatat actatcgctt tctgacggca gccgcttttg
     3901 gaatttctgc ctgtcattca cccgcggggg aagccaatga accggcaccg cctttcaacc
     3961 gggatagcct ggtaagccat atcatcatac tggcttcgga ttcatttcag ggccgcaaac
     4021 cctttactgc aggagaagaa aaaaccattg cctacctgca acagcaattc agctccatgg
     4081 gcctggaacc cggcaacggc aggagtttcc tgcaggatgt tcccctggtg gatatatcga
     4141 gaacaacaga cccggtaatg aaagtggaat cggcccgggg aagtttcccg ctgaaggacg
     4201 gcaaagatta cgtgttgtct acggaaagca ccgatccggt catttcgctg gataaggctg
     4261 atctggtctt tgccggctat ggcgtggtgg cgccggaata tcactggaat gattatgcgg
     4321 gaatttatgt gaagggtaaa gtggtcctgg ttatggtcaa tgatcccggg ttcggaacaa
     4381 ccgataccag tatttttaaa ggccggacca tgacctatta cggccggtgg acctataaat
     4441 tcgaagaagc cgcaagacag ggtgccaaag gctgcctgat cattcacaat accgcggcgg
     4501 cttcctaccc cttcagcgtg gtgcagagtt cctggggatc ttccagttta ttcctggatg
     4561 aaagaaaaaa cccggtttat cattgccctt ttgaaggctg ggtgagtgca gatgccgcaa
     4621 agaaattact tgccgcggcc ggaaaggaca gcagtctcct ggtgcaggca aataaacccg
     4681 ggtttaaagc ggttccgctt gaagagaaac tatccacctc catgcgggtg aaagcggtct
     4741 acaataactc caaaaacgtg atc
//
LOCUS       DASYCU010000253         6436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1215188, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000253 DASYCU010000000
VERSION     DASYCU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215188"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VGM24_05045"
     CDS             <1..928
                     /locus_tag="VGM24_05045"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY4154765.1"
                     /translation="GLQKQTGHSILYEAAVCASIPVIRNLEEYYDNDLLHSIRAIVNG
                     STNFILTKMFEEKLDFKQALLLAQQLGFAESDPSLDVEGFDAVNKWALLLTHAYGVVN
                     PPSSIVFNGIQNIQSGDATVAAEKHYDIKLVAQAKKLLNGKVAALVLPQFVKADDHLS
                     FVKNEYNGVVIESGFADKQFFYGKGAGSFPTASAVLSDISALRYQYQYEYKKLYHHAP
                     LQLADDFYVKAYISFDDWQYIPKEKFEWIEEWHAESERKYLIGVLPFKELQDKTWWRE
                     NKISLILAPEAIIEDVEIRKIRKKSLELAGIV"
     gene            1110..3488
                     /locus_tag="VGM24_05050"
     CDS             1110..3488
                     /locus_tag="VGM24_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154766.1"
                     /translation="MLKNYLKIAFRNLLRHKAFSAINILGLALGMACSLLIMLWVEDE
                     KAVDAFHANGKQLYQVYERDMNDGKVQAGYPTQGLLAEELKRVIPEVQFSAALDYAAS
                     PGTLNTFEAGNKVIKENGNFAGADFFSMFSYPLLQGNPVTALSEPAGIAISRKMAEEF
                     FGTPEQAMNKTLRFDNSENLQVRAVFENLPAHASRKFDFLRSWTDYVKRNDWVHNWGN
                     TSPDTYIQLRKTADPAKVEAEIKDFIYRYKPKDKGSSTELGLQSFTKKYLYSAFKNGK
                     PDGGRISYVHLFTLVAVFILLIACINFMNLATAQSAKRAKEVGLRKVIGAFRSSLIRQ
                     FLGEALLLTFFSVLLALVLAAALIPAFNQLTGKQLSIPFGQPGFWIVLSGLLIVTGFV
                     AGSYPALFMSSLNPVRVLKGSLKFGWQAAFFRKGLVVFQFVLSFLLIVGMIVIYRQVK
                     FIQSTNLGYDRENLLYIPIEGELVKRFELFKDEAGEIPAVLSISKMRNSPTIIEHHTG
                     SISWPGKDPNAQLSFADAVVGYDFVKTMKLKMKDGRDFSRDFAGDSLSYLLNETAVNK
                     IGFKNPVGQTVTWGNHPGKIIGVLRDFHFSSMHQSIDPLIVRLDENWPWGTILVRING
                     KHTREALAGLQKICAGLNPKFPFTYTFSDQEFANLYQSEQVVSQLANGFAVLAIFISC
                     LGLFGLATFTAGQRTKEIGVRKALGATSSSVLGLLSKDFLKPLLVAIAIATPLAWVVM
                     NRWLSNYAYKIRLDWWIFLLAGLLAVGIAMLTVSYQSIRAALMNPVTSLREN"
     gene            complement(3501..5405)
                     /locus_tag="VGM24_05055"
     CDS             complement(3501..5405)
                     /locus_tag="VGM24_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEY4154767.1"
                     /translation="MNRSAREILKEYWNYEHFRPMQEDIVQSVLSGKDVLALLPTGGG
                     KSICFQVPALLKPGICLVLSPLIALMKDQVQHLRSRGITAFSISSGMSRKDVIATFKL
                     AAAGNCKFLYVSPERLETDLFKEFLPALEVSLIAVDEAHCISQWGYDFRPPYLRIAAL
                     REELPGIPVLALTASATKEIQKDICLRLKMAEPVIYRQSFLRPNLSFSALRTSSVFNS
                     ITGILRKQDGSAIIYCRNRRRTKEISDLLNMHGLSADYYHAGLVPEQRSEKQDRWKKN
                     AVRVMVCTNAFGMGIDKPDVRLVLHADLPDCLENYYQEAGRAGRDGKKAFAILLYNDQ
                     MLEDLEMQHKTQFPPLDYIREIYQGLTNYLQVPLGTVNTYYAFDFIDFLKKFSFEARR
                     ANAALQVLGQEDLLSLNETVFKPATVYFICGKESLYAFELENPELEPLIKTLLRTYAG
                     IYDQPVPVHEKQLAFLLKWPAEQVLKGLHQLHRFQVIDYDPQKEEPQIYFNQPRRKAA
                     DVQIDQKTYAFRRQQFIKRARAMLGYIAGPDCRSTYIARYFGEESPEDCGICDRCLRN
                     QEPKLSAGQFALIYKTVTERIRQQSIPAASLFDHFPEIHAGQLRKVIDFIQAENKLRV
                     DADGFARVES"
     gene            complement(5427..>6436)
                     /locus_tag="VGM24_05060"
     CDS             complement(5427..>6436)
                     /locus_tag="VGM24_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY4154768.1"
                     /translation="ACPCAMGLATPAAIAVGMGRAARNGILFRRADSLELFKNIRQVV
                     FDKTGTLSTGSFMVSRFEILDQTLSEPDFKRILFSLEKYSNHPIAKSIARSWKSQPEI
                     RWTKLEEVKGLGIRASDAMGNEYKAGSAKFFPEETDSGHAIYISKNGRRIGWVDIQDE
                     LRPEAAEVVRYFRERNLRTILLSGDREEKCREIAAILGIDEVIAEQSPAQKLEQIERL
                     SAIGPTAMVGDGINDAPALAKASLGISMSEASQIAMQTADVVLMNSGLKKLPFSLQLG
                     KQTYLTIRQNLFWAFFYNIIAIPVAALGFLTPAVGALAMALSDVVLLINSSRLFVKKL
                     L"
BASE COUNT         1596 a         1687 c         1590 g         1563 t
ORIGIN      
        1 cggactgcaa aagcagactg gccattccat tttgtatgag gcggcggtct gcgcctccat
       61 accggttatc cgcaacctgg aagaatacta cgataatgac ctgctgcatt ccatccgggc
      121 tatcgttaac ggatccacga acttcatcct gacgaagatg tttgaagaaa aactcgactt
      181 caaacaggcg ctgctcctgg cacaacaact ggggtttgca gaaagcgatc cttccctgga
      241 tgtggaagga ttcgatgccg tcaacaaatg ggcgctcctt ctcacccatg cgtacggggt
      301 ggtcaacccc ccgtcttcga tcgtatttaa cggcattcag aacatacaat ccggcgatgc
      361 cacggttgct gccgaaaaac actatgacat caagctggtt gcccaggcaa agaaattact
      421 gaacggaaaa gtggccgccc tggtgcttcc gcagtttgta aaagcggatg accatctcag
      481 ctttgtaaag aatgagtaca atggagtggt catcgaaagc ggattcgcgg acaagcaatt
      541 cttttacggt aaaggagccg gcagtttccc gaccgcttca gcggtgttaa gcgatatctc
      601 cgcactccgc tatcaatatc aatacgaata caagaaatta tatcaccacg ccccgttaca
      661 gctggccgat gatttttacg tcaaagccta tatcagtttc gacgactggc aatacattcc
      721 caaagaaaaa ttcgaatgga tcgaagaatg gcacgccgaa agcgagcgca aatacctgat
      781 cggcgttctc cctttcaaag aactgcagga caagacctgg tggcgcgaaa ataaaatctc
      841 cctcattctc gcgcccgaag cgattattga agatgtagaa atccggaaga tccggaagaa
      901 aagcctggag ctggcaggga ttgtttgagc gcgcgggtca ggcgttttac aagcggacgg
      961 cctgtatcgt atgcggacag atctctttct gttgaggaat tttaaaaatc tgattaccag
     1021 tcgtttaaga tccggcattt ttttggaggc gtttcaaagg ccgcgctatc ggaaaaactc
     1081 cgccggcgcc catccttcaa ccagataaaa tgctaaagaa ctacctcaag atcgcgttcc
     1141 gcaatctcct ccgccataaa gccttttccg caatcaatat tctgggtctg gcgctgggca
     1201 tggcctgcag cctgctgatc atgctctggg tagaggatga gaaggccgtg gatgcctttc
     1261 atgccaacgg caaacagtta taccaggtat acgagcggga tatgaatgac gggaaagtgc
     1321 aggccggcta tcctacccag gggctgctgg cagaagaatt aaaaagggtg attccggaag
     1381 tgcagttttc cgccgcactc gattatgccg catcacccgg aactctcaat acattcgaag
     1441 ccgggaataa ggtcattaag gagaacggaa attttgccgg cgccgatttt ttcagcatgt
     1501 tcagttatcc cctgctgcaa gggaatcctg taacagcatt gagtgaaccg gccggtattg
     1561 ccatatcccg gaagatggca gaggagtttt ttggaactcc cgaacaggcg atgaataaaa
     1621 cccttcgctt tgacaatagc gaaaacctcc aggtaagggc ggtctttgaa aaccttcccg
     1681 ctcacgcatc ccgcaaattt gattttttaa gaagctggac agattacgtg aaacggaatg
     1741 actgggtgca taactggggc aatacgagtc cggataccta cattcagttg cgtaaaacag
     1801 cagacccggc aaaagtggaa gcagaaatca aagatttcat ttaccggtat aaaccaaaag
     1861 acaaggggtc ttcaaccgaa ctgggcctgc agtcctttac aaaaaaatat ctctattccg
     1921 cctttaaaaa cggaaagccg gatggaggcc gaatatccta tgtccacctg tttacgctgg
     1981 tcgctgtttt tatcctgctg atcgcctgca ttaattttat gaatctggca acggcccaat
     2041 cggctaaacg agccaaagaa gtcgggctgc gtaaagtgat cggcgccttc cgttcatcac
     2101 tgatccggca atttttaggg gaagccctgc tgcttacgtt cttctcggtt ctccttgccc
     2161 tggtcctggc tgcggccctg atacccgcat tcaaccaact caccggaaaa caactttcca
     2221 tacccttcgg acaacccggg ttctggatcg ttttgagcgg cctgctgatc gttacgggat
     2281 tcgtggccgg cagctatcct gccttattca tgtcatccct caatcccgtg cgggtattga
     2341 aagggagcct gaaattcggc tggcaggctg ccttcttccg gaagggccta gttgtttttc
     2401 agtttgtgct ttcctttcta ctcatcgtgg gcatgatcgt gatttaccgg caggtgaaat
     2461 ttattcaaag cacgaatctg ggttacgacc gggaaaacct tttgtatatc ccgatcgaag
     2521 gtgaactggt aaaaagattt gaactgttca aggacgaagc gggggaaata cccgccgtgc
     2581 tcagcatttc aaaaatgcgc aattccccca cgatcattga gcatcatacg ggaagcatca
     2641 gctggccggg aaaggacccg aatgcgcagc tttcctttgc cgatgccgta gtcggatatg
     2701 atttcgtaaa aacgatgaag cttaaaatga aggatggccg cgatttctcc agggattttg
     2761 caggcgactc cctcagctat ctattgaatg aaaccgccgt aaataagatc gggtttaaaa
     2821 atccggtagg ccagaccgtt acctggggaa atcatcccgg aaagatcatc ggcgtgctca
     2881 gggacttcca tttcagttcc atgcatcagt ccatagaccc gctgattgta agactggatg
     2941 aaaactggcc ctggggtacg atcctcgtgc gaataaacgg aaagcatacc cgggaagcac
     3001 tagcgggact tcaaaagatc tgtgccggat taaatccgaa atttccgttc acctatacct
     3061 tctccgacca ggaattcgct aatctttatc aaagtgaaca ggtggtttcg cagctggcaa
     3121 acggctttgc cgttctggcg atttttattt cctgtctggg tcttttcgga ttggcaacgt
     3181 tcaccgccgg ccagcgaacc aaagaaatcg gtgtgcgcaa ggccctgggc gcaacctcct
     3241 ccagcgtgct gggcctgctc tccaaagact tcctgaaacc attgctggtt gcgatagcga
     3301 ttgccacccc cctggcctgg gtggtaatga accgctggct atccaattac gcctataaaa
     3361 tccgcctgga ctggtggatc tttttactcg ccggtttact ggccgttggc attgcgatgc
     3421 ttaccgtcag ctaccagagc atccgggccg ccctgatgaa cccggtaacg agtttgaggg
     3481 agaactgaga ccccgctgct tcagctctcc accctcgcaa aaccatccgc atccaccctt
     3541 aatttattct ccgcctgaat gaaatcaatc actttgcgaa gctgcccggc gtgaatttca
     3601 ggaaagtgat caaacaagga agcggcggga atggattgct gccggattct ttcggtcacg
     3661 gttttataaa tgagggcaaa ctggcccgcg ctgagtttcg gttcctggtt tcgcaggcaa
     3721 cggtcgcaga tgccgcagtc ctcgggagat tcctccccga aataacgggc gatataagtg
     3781 ctccggcaat ccggcccggc aatatagccc agcatggccc ttgccctttt tataaattgc
     3841 tgcctgcgga acgcatacgt tttttgatca atctgcacat ctgccgcttt tctgcgcggc
     3901 tgattaaaat aaatctgcgg ttcttctttt tgcggatcat agtcaatcac ctgaaaacgg
     3961 tgcagttgat gaaggccctt taagacctgc tccgccggcc atttcaataa aaaggcaagt
     4021 tgtttttcgt gcacgggaac cggctgatca taaattccgg catacgttct caatagtgtt
     4081 ttgatcaggg gttccagttc cggattttcc agttcaaagg catagagcga ttcctttccg
     4141 caaataaaat aaacggtggc cggtttaaaa accgtttcat taagagaaag caggtcttcc
     4201 tggccgagca cctgcagggc cgcatttgcc cgcctggctt caaacgagaa ctttttcaga
     4261 aaatcgataa agtcgaatgc gtaatacgta ttgacggttc ccagcggcac ctgcaaataa
     4321 ttcgtcagcc cctgatatat ttcacggata taatccagcg gcggaaattg tgttttatgc
     4381 tgcatttcca ggtcttccag catctgatca ttatacaata aaatggcaaa cgcttttttc
     4441 ccgtcccgtc ccgcacggcc tgcttcctgg taataatttt ccaggcagtc gggcagatcg
     4501 gcgtgcagga ccagcctcac atccggtttg tcaatgccca tcccgaaggc gttggtgcag
     4561 accatcacgc gcaccgcatt tttcttccag cgatcctgct tttcgctgcg ctgttccggc
     4621 accagtccgg cgtgataata atcggctgaa agtccgtgca tattcagcag atcgctgatt
     4681 tcctttgtgc gtctccggtt ccggcaatag ataatggcgc ttccgtcctg cttgcggaga
     4741 atgcccgtga tcgaattaaa aactgagctt gtccgcagcg cactgaaaga aaggttgggg
     4801 cgcaaaaaac tctgccggta aataacgggc tccgccattt tcagccggag acaaatatct
     4861 ttctgtattt cttttgttgc cgaagcggta agcgccagta cgggaatgcc gggaagttct
     4921 tccctcagtg cggcaatgcg cagatagggc gggcggaaat catagcccca ttgtgaaata
     4981 cagtgcgctt catccacggc gatcaggctc acctccaggg ccggtaagaa ttctttaaac
     5041 agatccgtct ccagccgttc aggagaaaca tacaggaact tgcaattgcc ggcggccgcc
     5101 agtttgaaag tggcaatgac gtccttccgg ctcatgccgg aggaaatcga aaatgccgtg
     5161 atccccctgc tccggagatg ctgaacctgg tccttcataa gggcaatcaa cggactcagc
     5221 accaggcaaa ttcccggttt taacaaggca ggtacctgga agcagatgga tttgccgccg
     5281 ccggtgggaa ggagggccag cacgtctttc ccggataata cggactgaac aatatcttcc
     5341 tgcatgggcc tgaaatgttc atagttccag tattctttca ggatttcgcg cgcagaacgg
     5401 ttcatgcagt aaaatgaata ctaaaattac agtagctttt tcacaaataa ccgggaggaa
     5461 ttgatcagga gaaccacatc gctcagggcc atggccaggg ccccgaccgc cggggtgaga
     5521 aagcccaggg cagccaccgg aatggcaatg atattataga agaaagccca gaaaagattc
     5581 tgccggatgg tcagataggt ctgttttccc agctggagcg aaaacggcaa ttttttcaat
     5641 ccgctgttca tcagcaccac gtcagccgtt tgcatggcaa tctgagacgc ctcactcatc
     5701 gaaatgccca ggcttgcttt tgccagggcg ggagcatcgt tgatgccatc ccccaccatc
     5761 gccgtcggac cgatggcgga aagtctttct atctgttcca gtttttgtgc cggggactgt
     5821 tctgcgatca cctcgtcaat gcccagtatg gctgctattt cccggcattt ttcctcccgg
     5881 tccccgctca gtaagatcgt gcgcaggttc cgctccctga aatagcggac cacttctgcc
     5941 gcttccgggc ggagttcatc ctgaatatcc acccagccaa tccgccgacc atttttcgaa
     6001 atgtaaatag catgacccga atccgtctcc tccggaaaaa atttcgccga gccggccttg
     6061 tattcatttc ccatggcatc cgaagcccgt attcccaaac ctttcacttc ctccagcttt
     6121 gtccaccgga tttccggctg gcttttccag gagcgcgcaa tggattttgc gatgggatga
     6181 ttggaatatt tttcaagaga aaataaaatg cgtttaaaat caggctcgga aagcgtttgg
     6241 tccaggattt caaaccggct gaccataaaa gatccggtgc tgagggttcc tgttttatca
     6301 aataccacct ggcggatatt cttaaataat tccaggctat cggcacgacg gaaaagaatt
     6361 ccgtttcgtg cggcccgtcc cattcccacg gcaatggccg ccggcgtcgc cagccccatg
     6421 gcgcagggac aggcaa
//
LOCUS       DASYCU010000254         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3825383, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000254 DASYCU010000000
VERSION     DASYCU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3825383"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VGM24_05065"
     CDS             complement(<1..1166)
                     /locus_tag="VGM24_05065"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY4154769.1"
                     /translation="MAKKIKVTNPVVELDGDEMTRIIWKFIKDKLILPYVEVDIKYFD
                     LGIESRDQTNDQITVDAAHAIAKYGVGIKCATITPDEVRVKEFKLKQMWKSPNGTIRN
                     ILDGTVFREPIVIKNIPRLVPNWTAPIIVGRHAFGDQYRATDTVISGKGKLTMTFTPA
                     DGSAAKVFEVYDFKGDGVALAMYNTDESIRGFARSCFNMALSKKWPLYLSTKNTILKK
                     YDGRFKDIFEEIFQQEFKTAFQGAGITYEHRLIDDMVASALKWHGNFVWACKNYDGDV
                     QSDTIAQGFGSLGLMTSVLITPDGKILEAEAAHGTVTRHYRDHQKGKPTSTNPIASIF
                     AWTRGLAFRGKLDKNDELIRFCETLERVCIETVESGKMTKDLAVCLYGPKVNPG"
     gene            1310..1894
                     /locus_tag="VGM24_05070"
     CDS             1310..1894
                     /locus_tag="VGM24_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY4154770.1"
                     /translation="MEWIDNVSAYVSKYVILNGEELALLAGMFEMRNFNKRQLLVAEG
                     EVEQYLNFVIKGLARKFFHRRKEEMVTQFARENELICSYGSFLSGKPSNYAVEAIEPT
                     AVLSITKKNLDRLYESSPKMERLGRLVITEQFLSWEGFDYDRLRLSSSERFVNFIRSN
                     ADLLQRIPQKYLASYLNMKPETFSRLKHLVKGPL"
     gene            complement(1889..>3133)
                     /locus_tag="VGM24_05075"
     CDS             complement(1889..>3133)
                     /locus_tag="VGM24_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY4154771.1"
                     /translation="GIIGATVMPHNLYLHSALVQTRKIKREERYIRKALKLNFLDSSI
                     ALNMAFFVNAAILILAGAVFFKSGHAEVASIREAHELLSPLLGSKVASVLFAVALIAA
                     GQSSTVTGTLAGQIVMEGHLHLRINPILRRLITRMLAIIPAVFVIGVAGEQQVDALLV
                     LSQVILSLQLGFAVIPLIHFVSNKRTMGIFTINKKTKFFAWAIAALLVYLNLKFVIEM
                     GGGYFSQPGNGIWKILFTVVAVSALALLVYVFVHPWTGKPPETETAMHGKGSVITKLE
                     IPKFRKVAIALDFSGHDEKLLAFAIGQGGLESTYVLIHIVESASARMLGKQSDDLETR
                     NDQERLDKYQESLEASRYKAVTRLGYINRSREIVRIVKEEQAEMLVIGAHGHTGLKDF
                     IYGETVNTVRHELRIPVLIVNL"
BASE COUNT          747 a          794 c          747 g          845 t
ORIGIN      
        1 tctcccggat tcactttcgg accatataag cagacggcca ggtcttttgt cattttcccg
       61 ctttccacgg tttcaatgca tacccgttcg agggtttcac aaaaacgaat cagttcatca
      121 tttttatcca gcttgccccg gaatgccagt ccccgggtcc aggcgaaaat ggaagctatg
      181 ggattggtgg atgtgggttt ccccttttgg tgatcccggt aatgacgcgt tacggttcca
      241 tgggccgctt ctgcttccag gatcttgcca tccggcgtga tcagaacaga agtcatcaat
      301 ccgagagagc cgaatccctg cgcaatggtg tcgctttgga cgtccccgtc ataatttttg
      361 caggcccaga caaaattgcc atgccattta agcgcgctgg caaccatatc gtcaataagc
      421 cggtgttcgt aggtaatgcc tgccccctgg aatgccgttt tgaattcctg ctgaaagatc
      481 tcttcaaaaa tgtccttgaa ccggccatcg tatttcttca gaatggtatt tttggtggac
      541 aggtacaggg gccatttttt gctgagggcc atgttgaaac agcttctggc aaacccccgg
      601 atgctctcgt cggtattgta catggccagg gccacaccgt ctcccttaaa atcatatact
      661 tcaaatactt tggctgcgct gccgtccgcc ggagtgaagg tcatggttaa ttttcccttc
      721 ccgctgatca cggtatccgt tgcccggtac tgatctccga aggcgtgccg gccaacaatg
      781 atcggggctg tccagttggg aaccagccgg ggaatgtttt ttattactat aggttcccgg
      841 aatacggttc cgtcaagaat atttctgatg gtgccgttcg ggctcttcca catttgtttc
      901 agcttaaatt cttttacccg gacctcatcc ggcgtaatgg tggcgcattt tattccgacc
      961 ccgtattttg caatggcatg agccgcatct acggttatct gatcgtttgt ttggtccctg
     1021 gattcaatac ccaggtcaaa atatttaata tccacctcca catagggtaa aatcaatttg
     1081 tcttttatga atttccagat gatccgggtc atctcatccc catcgagttc caccaccgga
     1141 tttgttactt taattttctt tgccatgaaa acggaatttt tatttcaaaa gtcagcaaac
     1201 ctacatgaaa tgaatcaatt cacaaagcat ccgccggggg ctgccatata tcatatatat
     1261 ttccttctgc cgttttatca tggtattttg cacagttaaa atggtgccca tggaatggat
     1321 tgataacgta agcgcttacg tatcaaaata tgttattttg aatggcgagg agcttgcgct
     1381 tctcgcgggg atgtttgaaa tgcgtaattt taataagcgc cagttgctgg ttgcggaagg
     1441 tgaggtggaa caatatctca attttgtcat taagggcctt gcccggaagt ttttccaccg
     1501 caggaaagaa gagatggtca cccagttcgc cagggaaaat gaactgatct gctcctatgg
     1561 ttcctttctc tccggaaaac cttccaatta tgcggtggaa gcgattgagc ccacggcggt
     1621 cctttccatc acaaaaaaaa acctggaccg cttatatgaa tccagcccga aaatggaaag
     1681 gctgggcagg ctggtcatca cggaacaatt tctcagttgg gaaggatttg attatgaccg
     1741 gctccggctg agttcgtcgg agagatttgt aaattttatc cgcagcaatg cagatctttt
     1801 gcagcgcata ccgcagaagt acctcgcctc gtatctgaac atgaagccgg aaaccttcag
     1861 ccggttgaag cacctggtca aagggccatt ataaatttac aatgagcacg ggtatcctca
     1921 gttcatgccg cacggtattt accgtttctc cgtaaataaa atctttgagc ccggtatgcc
     1981 cgtgggcgcc gatcaccagc atttcagcct gctcttcctt cacaatgcgg acaatttccc
     2041 ggctccggtt gatatatccc agccgggtca cggctttata ccggctggct tcgagagatt
     2101 cctgatattt atccaaccgc tcctgatcgt tccgcgtttc caggtcatca ctctgtttgc
     2161 cgagcatacg cgcagaagcg ctttccacga tatggatcaa tacataggtg ctttccaggc
     2221 ctccctggcc aatggcaaag gcgagcagtt tttcatcatg cccgctgaag tccagcgcga
     2281 tggcgacctt tctgaatttc gggatttcca gtttggtgat gacagaaccc tttccatgca
     2341 tagccgtttc cgtttctggt ggtttgccgg tccagggatg aacgaataca tagactaaca
     2401 gcgccagggc cgaaaccgca acaacggtaa acaatatttt ccagatcccg tttccgggct
     2461 gactaaaata accgccgccc atttcaatca cgaatttaag attcaggtaa accagcaaag
     2521 ccgcgatggc ccaggcaaaa aatttagtct ttttattaat ggtgaaaatg cccatagttc
     2581 ttttattgct tacgaaatgg attagcggaa tgaccgcaaa gcccagctgc aggctaagga
     2641 ttacctggct caataccagg agtgcgtcca cctgttgttc gccggccacc ccgatcacga
     2701 acacggcggg aataatggcc agcatccggg ttatcagcct tctcaggatc ggatttatcc
     2761 gcagatgcag gtggccttcc attacgattt gcccggccag cgtgccggta acggtggaac
     2821 tttgtcctgc ggcaatcagg gcgaccgcaa aaagaacgga agccactttg gaacccagga
     2881 gcggggataa tagttcatgg gcctcccgga tggaagctac ctcggcgtgg cccgatttga
     2941 aaaagaccgc tccggccagg atcaatatgg ccgcatttac aaaaaaagcc atattcaggg
     3001 cgatgctgct gtccaggaaa ttcagcttga gcgctttccg gatataacgc tcttctcttt
     3061 ttattttccg ggtttgaacc agggcggaat gcaggtaaag attgtggggc attaccgtag
     3121 ccccgataat ccc
//
LOCUS       DASYCU010000255         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3102519, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000255 DASYCU010000000
VERSION     DASYCU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3102519"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VGM24_05080"
     CDS             <1..538
                     /locus_tag="VGM24_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY4154772.1"
                     /translation="AFKNLVYTPNFQLAEIKLEGKNYRCILKDIQFNKVTDSLDHVDF
                     LELVEGKKVTATIPLKFTGAAKGVKDGGKLITKMKALKVKAYPKDLRENIEVDLTDLE
                     LNGNVRVEDVKAADYEILNSPRIPVASIVLTRQLKQEETAAPAAGAAAAPAAGAAAAA
                     PAAAGKPGAAAKPASSKK"
     gene            653..1231
                     /gene="pth"
                     /locus_tag="VGM24_05085"
     CDS             653..1231
                     /gene="pth"
                     /locus_tag="VGM24_05085"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY4154773.1"
                     /translation="MSSFLIAGLGNIGSEYDGTRHNIGFDVVAALVAKEQGSFSIDRL
                     AAIAEIKFRGKMLTCILPSTYMNLSGKAVKYWLDRKKIPLERLLVITDDIALPLARLR
                     LRPSGSDAGHNGLKSIFEELQTDKYPRLRYGIGNNFPRGKQVEYVLGKWEPEELALVK
                     TKTEMAVRVIESFTVTGLERTMNDYNKIVITL"
     gene            1374..1985
                     /locus_tag="VGM24_05090"
     CDS             1374..1985
                     /locus_tag="VGM24_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4154774.1"
                     /translation="MKIFCVGRNYVAHAAELNNEVPEEPIVFMKPKSALLQAHTPFYY
                     PEFTNELHYECELVLRISKNGKYIQERFSPKYYDAISAGIDFTARDIQNELKEKGLPW
                     EKAKAWDNSAAVGKWIPIQNVKNKKEINFCLYKNKELVQQGSSVLMLNNFDKIVAYIS
                     TYFSINIGDLIFTGTPAGVGECVVGDELEAFIEDESLLNLEIK"
     gene            2044..>2877
                     /locus_tag="VGM24_05095"
     CDS             2044..>2877
                     /locus_tag="VGM24_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY4154775.1"
                     /translation="MTDSGILLKAYQHMCTARAMADTYERNRSVCKYVHSVSSGHEAI
                     QLAAAFHLQPPDYVSPYYRDESLLLGLGFSPYELMEQLLAKGEDAFSGGRAYYSHPNF
                     RGAGKPTIIHQSSATGMQAIPATGIAQGISFKEKCGMAEPGPIVLCSLGDASMEEGEV
                     SEAIQFAVLKQLPVIYLVQDNDWGISVTAEESRSMDAFTYAGGFPGLERIKIDGADFE
                     QSFHCLQGVFDFVRKCRKPYLVHARVPLLSHHTSGVRMETYRTEADLAQHRTMDPLES
                     LK"
BASE COUNT          753 a          691 c          736 g          697 t
ORIGIN      
        1 ggctttcaag aaccttgtat acacaccgaa ttttcagctc gcagaaatca aactggaagg
       61 gaagaattat cgctgcattc tgaaggacat ccagtttaac aaggtgaccg acagcctgga
      121 tcacgtggac tttcttgaac tggtggaagg caaaaaagtt acggccacca tcccgctgaa
      181 attcaccggt gccgcaaaag gagtcaagga cggcggaaag ctgatcacta aaatgaaggc
      241 cctgaaagtg aaagcctatc caaaggacct ccgcgaaaat atcgaagtgg atcttaccga
      301 cctcgaactg aacggaaatg tgcgggtgga agacgttaag gcggctgact atgagatcct
      361 gaattcgccc cgtattccgg tcgcctctat cgtgctgacc cgtcagttga aacaggaaga
      421 aacggcggct cccgctgcag gcgctgccgc tgctcctgcc gccggcgctg ccgctgccgc
      481 acctgctgcc gctgggaaac cgggggcagc cgccaaaccg gcgtcttcaa aaaaatagta
      541 aaccggttta tttgtttcga agggaggctg ttgcaaactt ttttggaaca gcctcccttg
      601 ttttaagacc cgccgcattt ttccccttca tgaattcctt tacatttgat gtatgtcatc
      661 tttcctgatc gccggactgg gcaatatcgg ttctgaatac gatggaacgc gtcataatat
      721 cggattcgat gtagtggccg ccctggttgc caaagaacag ggttctttct ctatcgaccg
      781 gctggcggcc attgctgaaa ttaaattcag gggaaagatg ctcacctgca ttcttccgtc
      841 cacctatatg aatctcagcg ggaaggccgt taaatactgg ctggaccgga aaaagatccc
      901 tttggaacgc ctgctggtga tcacggatga tattgccctg cccctggccc ggttacgcct
      961 gcggccatct ggaagtgatg cgggacacaa tggcctgaag agcatcttcg aggaattaca
     1021 aacggataag tacccgaggc ttcgctacgg gatcgggaat aactttcccc ggggaaaaca
     1081 ggttgaatat gttttgggaa aatgggagcc ggaggaattg gccctggtta aaacaaaaac
     1141 ggaaatggcg gtccgggtga ttgaaagttt tactgtgacc gggttggagc ggaccatgaa
     1201 tgattataac aaaatagtga ttacgttatg atttgtaatt gggaatcatt attttagcgt
     1261 tcaaatccgc ggtatagccc gcggatattc atattcaatc tcctatggtg ttttctcatg
     1321 ccgttccagt atccggaaca gagcaaatct tctatttaaa accttaattg aatatgaaaa
     1381 tattctgcgt tggtcgaaac tatgttgcgc atgctgctga gttgaataat gaagtgcctg
     1441 aagagcccat cgtcttcatg aaacccaaga gtgccctgct tcaggcccat actccttttt
     1501 attaccctga atttaccaat gaactccatt acgagtgtga gctcgtgctg cggatttcaa
     1561 aaaacgggaa atatatccag gaacgatttt ccccgaaata ttatgatgcc atcagcgcgg
     1621 gcatcgattt tacggcaagg gatattcaga atgagctgaa agaaaagggc cttccctggg
     1681 aaaaagcaaa agcctgggac aactccgcgg cggttggcaa atggatcccg attcaaaatg
     1741 taaagaacaa aaaggaaatc aatttctgcc tctataaaaa taaagaactg gtgcagcagg
     1801 gaagttccgt cctgatgctt aacaatttcg acaagatagt cgcctatatt tccacctatt
     1861 tttcgatcaa tatcggcgac ctgattttta cgggcactcc ggccggcgtg ggagaatgtg
     1921 tggttggaga tgagctggaa gccttcattg aagatgaaag cctgctgaac ctggagataa
     1981 aataatccgg agaaagaacc ggctttcttc ccggcaaagg ttttttaatt taagaattcc
     2041 cgcatgaccg attccggtat cctgctcaaa gcatatcagc atatgtgtac agcccgggca
     2101 atggctgata cctacgagcg caaccggtcc gtttgcaaat atgttcattc ggtttcttcc
     2161 gggcacgaag ccattcagct cgctgccgct tttcatcttc agccaccgga ttatgtgagc
     2221 ccttattacc gcgacgaaag ccttttgctc ggccttggat tttctcccta tgaactgatg
     2281 gaacagctac tcgccaaggg agaagacgcc ttctccggtg gaagggcata ctattcgcat
     2341 cccaatttcc ggggtgcggg taaacccacc atcattcacc agagcagtgc gaccggcatg
     2401 caggcgatcc cggccaccgg tattgcgcag gggatttcat tcaaagaaaa atgcggcatg
     2461 gctgaaccgg gacccatcgt gctctgctcg ctcggagatg cgagcatgga agaaggggaa
     2521 gtaagtgaag ccattcagtt tgccgttttg aaacagctgc ccgttattta tctcgtgcag
     2581 gataatgact ggggcatcag cgttaccgcg gaagaatccc gcagcatgga tgcctttacc
     2641 tatgccggcg gatttcccgg tctggaaaga atcaaaatag acggagcgga ttttgaacag
     2701 tcgtttcatt gcctccaggg ggtatttgat tttgtaagaa aatgccggaa gccctacctg
     2761 gtgcatgcgc gggttcccct gctgagccat cataccagcg gtgtccggat ggagacgtac
     2821 cggacggaag cagaccttgc ccaacatcgt acaatggatc cgctggagtc gctaaaa
//
LOCUS       DASYCU010000256         7556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1655345, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000256 DASYCU010000000
VERSION     DASYCU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655345"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..2192)
                     /locus_tag="VGM24_05100"
     CDS             complement(72..2192)
                     /locus_tag="VGM24_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEY4154776.1"
                     /translation="MELRRDILWRVYLCFLGIVVICIAVLGRVFYIQQIQGSYWRGMS
                     DSLHQKYIDLNAERGTIYSEDGNMLSTSIPYFNIYIDFAADGLREKNGKRFKDNVDSL
                     SICLASLFRDQSATAYKKDLQQGYKKEDRYYSLRKNLTFDQYKQLREFPLVKQGRNKS
                     GFIIETTSKRLNPFGLLANRTIGLSRNNAQNVGLERTYDTLLKGENGRRLVRYMAAGA
                     YIPVEGYEIDPENGKDIVTTIDVNTQDIAENALLKMMQANECEHGTCLVMEVATGKIR
                     AIANLGKQPDGSYWEDLNYAIRATEPGSTFKLATLLSLLEDHDVKLDDRVNLEGGKWK
                     VGRRTVYDSEKHGEGEYTVKQAFELSSNVGMAKLAVAHYGGHPQQFTDHLKKLKLNQI
                     TGIDLAGETQPIIKSPKSRTWSATTLPWMAFGYEVLVSPLQTLTLYNAVANQGKMMRP
                     YLVNAIEDAGGIEKQNQPVVMLDKICSDRTLAELQSCLEGVCIEGTAKGIFRNSFYPV
                     AGKTGTALVANGTRGYADHIYQSSFVGYFPANRPKYSCIVVIKNKPFAKKYYGGDVAG
                     PVFREVADKLMSLERDNPDNSRFAALTELMKKDSSKYYYAGNTKEMKSVLDLLGISFV
                     DSSGGKTWAGLYPVNNLPVVSTKTEVAKRMPDVKGMGLKDALYLLESRAVQVIVKGAG
                     KVSQQNIPAGTAIEKNQKLTIELN"
     gene            complement(2220..2519)
                     /locus_tag="VGM24_05105"
     CDS             complement(2220..2519)
                     /locus_tag="VGM24_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HEY4154777.1"
                     /translation="MRRILRYQWMLRNLPFFFFLAFLAVLYIYNGHYADHVVRDIGKT
                     GKELKELQYEYKSLKSEVMFRSKQSELAKAVEPFGLKELSSPPVILADSSESGTK"
     gene            complement(2631..3554)
                     /gene="rsmH"
                     /locus_tag="VGM24_05110"
     CDS             complement(2631..3554)
                     /gene="rsmH"
                     /locus_tag="VGM24_05110"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY4154778.1"
                     /translation="MNYMTGSSPAYHIPVLLSEAMDGLDIRANGIYVDCTFGGGGHSA
                     EILSHLNNEGRLYAFDQDTDAKQNIPKDERFHFIPQNFRHLHRFLRVNGVDAVDGVLA
                     DLGVSSHQFDETERGFSTRTDASLDMRMDKRQSLTAAEILNVYPEADLHRLFERYGEV
                     TNSKTLARSIAALRQTNPFRTVNGFRHLIAPLVKGNPNRYFAQVFQALRIEVNDEMGV
                     LREMLEQLPALLKPGGRAVIISFHSLEDRMVKQFFRDNIFEQQDNPDPYGTRAAKTLR
                     MINKKPITPSETELKQNPRARSAKLRIAEKI"
     gene            complement(3551..4006)
                     /gene="mraZ"
                     /locus_tag="VGM24_05115"
     CDS             complement(3551..4006)
                     /gene="mraZ"
                     /locus_tag="VGM24_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY4154779.1"
                     /translation="MNGFLGEYEVTLDPKGRFLLPASLKRQTGEEGSQFVINRGFEKC
                     LGLYPMKSWEPIFEEISKLNDFDPKVRSFRRYFLNGATMMELDSAGRLLIPQSLKEYA
                     GLEKDIVLVTAVSKIEIWDKTKYQQFFESFSPDDFSGLANEVMNGKPDK"
     gene            4285..5505
                     /locus_tag="VGM24_05120"
     CDS             4285..5505
                     /locus_tag="VGM24_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEY4154780.1"
                     /translation="MSDLFPENLSISEFQYDLPEDKIARYPLPQRDASKLLIWQGGTI
                     SETRYARIADWLPAASLLVFNNSKVIAARLLFSKPSGGVIEIFCLEPADSGTGIHEAM
                     VRKENGKWNCLIGGASKWKRGMVLEKKAGQTILRACFLEKKPDSFVVEFSWSPAPLSF
                     AEVLHLLGSVPLPPYLKRHAEAADAERYQTIYAAEEGSVAAPTAGLHFTPQIFDSLGQ
                     KKIETLFVTLHVGAGTFMPVKTDALADHLMHEEYIEVRAETVRRLLDRLSGPVIAVGT
                     TSLRTVESLYWLGLKVAENKRIPADALRISQWDPYRMDSALEAKKALHLLLEWIERQP
                     DQKLITKTQLFIAPGYSFRIIRGLITNFHQPQSTLLLLVAALAGNDWKRIYQFALEND
                     YRFLSYGDGCLFLP"
     gene            complement(5470..6855)
                     /locus_tag="VGM24_05125"
     CDS             complement(5470..6855)
                     /locus_tag="VGM24_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4154781.1"
                     /translation="MKKNFTIIFILISVSLIGIIYIQWNWITTMVENKEEELHHKLVD
                     VTTDVARDLIEYKQTALSGSALLRSAPGFNWRPTEQFLRELMRPPLISQKFSYEELRD
                     KLKRAFVYRGLKDTKFEFCIFSEIGVNTAMGSDTYELKSPNFFAAFTDTVHNKQTMLP
                     LVTSSSELTGPEPFETLSIIIPDYKSVVMKQMRLMIFVVIFFTLIIISAFYVTVRTLL
                     RQKKLSEIKNDFINNMTHEFKTPLATISLAVDAMRNEKVVHDRGKSEYFSNIIKEENR
                     RMNKQVETILQAALIDRQELQLKLQPIYAHEVIRRVMDNFALQFQEINAVVKLELNAG
                     YDLMEADEVHFTNLISNLIDNAIKYSRDKLIIRISTQCSGKYLSIRIEDNGIGMNKET
                     QRRIFEKFYRAHTGNVHNVKGFGLGLSYVKSVVDAHHGRIKVDSSVGKGTVFTLEMPL
                     LRQEQASVAIT"
     gene            6956..7507
                     /locus_tag="VGM24_05130"
     CDS             6956..7507
                     /locus_tag="VGM24_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY4154782.1"
                     /translation="MDHPAPGILLIADPFLKDPNFKRSVVFLCEHQSEGSFGFLLNKT
                     YEYSLKDLVQNAEDLEIPVYFGGPVQTDTIHFLHQYPDLIQGGYEVTDGIFWGGDFEK
                     ALALIRSGQIKAGKIRFYIGYSGWAGGQLDDELKEKSWLPVKASRKLIFHKKTEEIWK
                     EAIRQLGGNYLMMINFPTDPQLN"
BASE COUNT         1776 a         1937 c         1754 g         2089 t
ORIGIN      
        1 cttcacattt acctgaaggt ttccgtgaac ggatcgtata gccaccttat ataatatttc
       61 ctgcagattc atcagttcag ttctatggta agcttttgat ttttttcaat ggcggttccg
      121 gccggtatat tctgctggct taccttcccc gctcctttta ctattacctg gaccgctctg
      181 gattccagca ggtaaagggc gtccttcaat cccattccct ttacatcagg catccgtttg
      241 gcgacttccg tttttgtgct gaccaccggc aggttattta ccggatacaa acccgcccag
      301 gttttaccgc cagacgaatc cacgaaagaa atgcccagca ggtccagtac agatttcatt
      361 tcctttgtat tgccggcata ataatacttg gaactgtctt tcttcatcag ttccgttaat
      421 gcggcaaacc ggctgttatc cgggttatcc ctctccaggc tcatcagttt atcagccacc
      481 tcgcgaaaca ccggcccggc aacgtcccct ccataatatt tttttgcaaa tggtttattc
      541 ttgatcacca cgatacagga atacttgggc cggttcgcag gaaagtatcc gacaaaagaa
      601 gactggtaga tatggtctgc atatcccctg gttccgttgg cgaccagggc cgtgcctgtt
      661 tttccggcaa ccggataaaa tgaattcctg aaaatccctt tggcggttcc ttcgatacag
      721 accccttcga ggcagctctg cagttctgcc agcgttctgt cgctgcagat tttgtcgagc
      781 atcactaccg gctgattttg tttttcaatg ccgcccgcat cctcgatggc attcaccagg
      841 taaggcctca tcatttttcc ctggttggcc accgcgttgt ataaagtaag ggtctgcagg
      901 ggactgacca gcacctcata gccgaatgcc atccagggaa gggttgtcgc gctccaggtc
      961 cgggacttcg ggctcttgat aatgggttgc gtttctcccg cgaggtctat gccggtgatc
     1021 tgattgagct ttaatttctt caggtggtcg gtgaactgct gcggatgacc gccatagtgg
     1081 gccacggcga gtttggccat tcctacattg gaactcagct cgaaggcctg tttcaccgtg
     1141 tattcccctt cgccgtgttt ctctgaatca tacaccgttc gcctgcccac cttccatttc
     1201 ccaccctcca ggttcacccg gtcatcaagt tttacatcat ggtcttccag cagggaaagt
     1261 aaagtggcga gcttgaaagt ggaaccgggt tcggtggccc ggatcgcata attgaggtct
     1321 tcccaataac tcccatccgg ttgttttccc aggttcgcaa tggcccggat ctttccggtg
     1381 gccacttcca tgaccaggca ggtgccgtgc tcgcactcat tcgcctgcat cattttcagg
     1441 agcgcatttt cggcaatatc ctgtgtgttc acatcgatgg tggtcacaat atccttcccg
     1501 ttttccgggt cgatctcata tccttcaacc gggatatagg cgccggcggc catatagcgc
     1561 accagtcttc ttccgttttc tcctttcagc aaagtatcgt aagtcctctc caggccaacg
     1621 ttctgcgcgt tgttgcggga cagtccgatg gtgcggttgg cgagcagacc aaaagggttc
     1681 aaccgcttac tggtcgtttc tataatgaat ccgcttttat ttctgccctg ttttaccaat
     1741 ggaaattctc ttagctgttt atattggtcg aatgtgaggt tcttccggag cgaataatac
     1801 cggtcttctt ttttatatcc ctgctgcagg tcctttttat atgcggtggc tgactggtcc
     1861 cggaaaagag aggcaagaca tatggacaga gagtccacat tatctttaaa ccgtttcccg
     1921 tttttttccc ggagcccgtc ggccgcgaaa tcgatataga tattgaaata aggtattgac
     1981 gtactcagca tatttccatc ttcactgtaa atcgttcccc tttccgcatt gagatcaata
     2041 tatttctggt gcaggctgtc gctcattccc cgccagtagc ttccctggat ctgctgaata
     2101 tagaaaacac gtcccagcac ggcgatacag atgaccacga ttcccaggaa acacaggtaa
     2161 actctccata gtatgtctct tctcaattcc accgggtatc ggggatatgg ttataaaaat
     2221 tatttcgttc cgctttcgct gctgtcggca agtataaccg gtggggagga cagctctttc
     2281 agtccaaaag gctccacggc ttttgccagt tcactttgtt tgctgcggaa catcacttca
     2341 ctcttcaggc tcttgtattc gtattgcaat tccttcagct cctttcccgt ttttccgata
     2401 tcccgcacca cgtgatctgc ataatgaccg ttatatatat agagcaccgc caaaaaagcc
     2461 agaaaaaaga aaaaaggaag gttgcggagc atccactgat agcggagaat gcgtctcact
     2521 ttttgccttt gattattccc gttgactttc atttgatttc ggcctgatgt taaaaattaa
     2581 agggtaccag attatcaaac tgacgtttcg tagatatgga tttcagtctt tcatattttt
     2641 tcggctatcc gcaatttggc gcttcttgca cgggggttct gcttcaactc cgtctccgaa
     2701 ggagtgattg gttttttgtt aatcatccgc aatgttttgg ctgctcttgt tccgtaggga
     2761 tccgggttat cctgctgttc aaaaatattg tctctgaaaa attgtttcac catccggtct
     2821 tccagcgaat gaaaactgat gatcaccgcc cgtccgccgg gtttgagcag ggccggcagt
     2881 tgttccagca tttcccggag cacgcccatc tcatcattca cttcgatgcg caacgcctga
     2941 aaaacctgcg cgaaataccg gtttggattt cctttaacca atggagcgat caggtgccgg
     3001 aagccattca cggtgcggaa cgggttggtc tgccgtaacg cggcaatgga tctggcaagc
     3061 gtttttgaat tggtcacttc cccataccgc tcaaatagcc ggtgcaaatc ggcctccggg
     3121 taaacattca gaatttctgc cgccgtgagc gattggcgtt tgtccatgcg catgtccagg
     3181 ctggcatccg ttcgcgtgga aaaaccacgt tcggtttcat cgaactgatg actgctcacc
     3241 cccaggtccg ccagcacacc atccaccgca tcaactccgt tcacgcgcag gaaccggtgc
     3301 aggtgcctga aattctgagg tatgaaatga aaacgctcat ccttcggaat attctgcttt
     3361 gcgtcggtat cctgatcgaa tgcatacagc cgtccttcgt tattcaggtg cgacaggatc
     3421 tccgccgaat ggccacctcc gccaaaggtg caatcaacgt agatcccgtt cgccctgata
     3481 tccaaaccgt ccatcgcttc tgaaagcaat acgggtatat gatacgcggg agatgagccc
     3541 gtcatataat tcatttatca ggtttaccat tcataacttc attggccagg ccgctgaaat
     3601 catccggtga aaagctttca aagaactgtt ggtatttagt tttatcccag atctctattt
     3661 tgctgaccgc cgtaaccagc acgatgtctt tttccaatcc cgcatattcc ttcaacgact
     3721 gggggatcag caaccggccc gccgaatcca gctccatcat cgtggctcca ttcaaaaaat
     3781 atcgccggaa tgaccttacc ttcggatcaa aatcattcag cttgctgatc tcttcaaaaa
     3841 tgggctccca gcttttcatg ggatacagac ccaggcattt ttcaaatccc cggtttatca
     3901 caaactggct gccttcctcc ccggtttgcc ttttgagcga agccggcagg agaaaccgcc
     3961 cttttgggtc gagcgttact tcgtattccc cgagaaaacc attcatttat aaggacttga
     4021 aacaatgttt ttaataattc acacaaaata acactttttc ccacttatca aacaaatttg
     4081 atggttttta atttttccac aagaaataaa tgcgttcaaa gcctgtggga gtaagggttt
     4141 accccgattt tccggcttgt ttccggaaat ttcctgtgta taagatgtga aaaggggtca
     4201 acagcggtgg ataagcctta agtcccgggc ctcccttttc agcagctccc gttttcttgc
     4261 gatttttgaa ctgaattctt acccatgtcc gatctatttc cagagaatct ttccatttcc
     4321 gaattccaat acgacctgcc ggaagataag attgcccgtt atcctttacc ccagagagat
     4381 gcgtcaaaac ttttgatctg gcagggcgga acgatttccg agacccggta tgcgcggatc
     4441 gccgactggc tgcccgctgc ttccctgctg gtgtttaata acagcaaggt gatcgctgcg
     4501 cggcttcttt tcagcaaacc ctccggtggc gtcatagaga tcttttgcct ggaaccggca
     4561 gactccggaa ccggtatcca cgaagccatg gtccggaagg aaaacggaaa atggaactgc
     4621 ctgatcggcg gggcctccaa atggaaaaga ggtatggtgc tggagaaaaa agcggggcaa
     4681 accattttac gggcctgctt cctggagaaa aaaccggatt cattcgtggt agaattttcc
     4741 tggtcgccgg cacctctttc gtttgcggaa gtacttcacc tgctcggctc cgtgccgctt
     4801 cctccctatc tgaaacggca tgcagaagca gccgacgcgg aaagatacca gaccatttat
     4861 gctgcagaag aaggatccgt ggcagcaccg accgccggac tgcatttcac gccgcagatc
     4921 tttgattcac tcgggcaaaa aaagattgaa actctgttcg tcacgctgca tgtcggcgcc
     4981 ggcaccttta tgccggtgaa gaccgatgcc ctggccgatc acctcatgca tgaagaatat
     5041 attgaagtgc gggcggaaac ggtaaggcgc ctgctggaca gattatccgg tccggtcatt
     5101 gcggtaggca ccacctcgct aaggacggtg gagagtcttt actggcttgg attgaaagtg
     5161 gcggagaata aacgcatacc ggccgacgca ctaaggatca gccagtggga cccttaccgc
     5221 atggattccg ccctggaggc aaaaaaagcg ttgcatttat tactggaatg gattgaacga
     5281 caaccggatc aaaaactcat cacgaaaacc cagctgttta tagcgcccgg ttattccttc
     5341 aggatcatcc gcgggctcat caccaatttt catcagccgc agtccaccct gcttctcctg
     5401 gtagctgccc tggcgggaaa tgactggaag agaatatatc agttcgcgct ggaaaatgat
     5461 taccggtttt taagttatgg cgacggatgc ctgttcctgc cgtaataaag gcatttccag
     5521 cgtaaagacc gttcccttac ccaccgagct gtccactttt atccggccat ggtgcgcatc
     5581 caccacggat tttacataac tcagccccag gccgaatccc tttacattat gaacgttccc
     5641 ggtgtgcgcg cgatagaatt tttcgaagat ccgccgctgt gtctccttat tcattccgat
     5701 cccgttgtct tcgatgcgga tgctgaggta ttttccactg cactgcgtgc tgatccggat
     5761 gatgagttta tccctggaat atttgatggc attgtcgatc aggttcgaaa tcaggttggt
     5821 aaaatgcact tcatcagcct ccatgaggtc gtacccggca ttcagttcaa gtttaaccac
     5881 agcgttaatt tcctgaaatt gcagtgcaaa attatccatc acccggcgga tcacttcgtg
     5941 ggcataaata ggctggagct tcaattgcaa ttcctgccgg tcgatcaggg ctgcctgcaa
     6001 aatggtttcc acctgtttgt tcatgcgcct gttttcttcc ttgatgatat tgctgaagta
     6061 ttctgatttt ccccggtcgt gcactacttt ctcgttccgc atggcatcca ctgccaggga
     6121 aatggtggca agcggcgtct tgaactcgtg ggtcatgtta ttgataaaat catttttgat
     6181 ctcactgagt ttcttttgcc ttaacagggt gcgcaccgtt acataaaaag cggaaataat
     6241 gatcagcgta aaaaagatca ccacgaaaat catcaaccgc atctgtttca tcacgacgga
     6301 cttataatcg ggaatgataa tagacagggt ttcgaaaggc tcgggtcccg taagctcgct
     6361 gctgctggtg accaggggaa gcatggtctg cttgttatga acagtatcgg tgaacgcggc
     6421 gaaaaaattg ggcgatttca attcgtaggt gtcggaaccc attgcggtat tgacccctat
     6481 ttccgaaaat atgcaaaatt cgaatttggt atctttcagt ccgcggtaaa caaatgctct
     6541 tttgagttta tcccggagtt cttcatagct gaatttctgg gagatcagcg gcggccgcat
     6601 cagttcccgc aagaactgtt ctgtcggccg ccagttaaag ccgggagcgg agcgaagcaa
     6661 cgccgaaccc gaaagcgccg tttgtttata ttcaatgagg tcccgggcca catcggtggt
     6721 tacgtcaacc agtttgtggt gcagttcttc ctctttattc tccaccatgg tggtgatcca
     6781 gttccactgg atataaataa ttccaatcag cgaaacactg atgagaataa aaatgatagt
     6841 gaagtttttt ttcacgcaga ctttgtttat accccaataa aaccggccga acagggtatg
     6901 ctaacagatt gcaaatttat taaacaatat cctttggatt agttaattta cgtatatgga
     6961 ccatcctgct cccggcatat tgctgatcgc cgatcctttt ctgaaagacc cgaattttaa
     7021 aagatccgtg gtttttcttt gtgagcatca gagcgaaggt agttttggtt tcctcctgaa
     7081 taaaacatac gaatatagtc tgaaagacct ggttcagaat gcagaagatc tggaaattcc
     7141 ggtttatttc ggcggaccgg ttcaaacgga taccattcat tttctccatc agtaccccga
     7201 cctgatccag ggcggttatg aggtgacgga tggaatcttt tggggaggag attttgaaaa
     7261 ggcgctggcc ctgatccgtt ccggtcaaat caaggctggc aaaatccgtt tttatatcgg
     7321 ctattccggg tgggccgggg gccagttgga cgatgaactg aaagaaaaat cctggctgcc
     7381 ggtcaaagcg agcagaaaat tgatcttcca taaaaaaaca gaggagatct ggaaagaggc
     7441 catccggcag cttggcggaa attatctgat gatgatcaat ttcccaacgg atccgcaatt
     7501 aaattaaacc tgtttcgccc cctctaccaa aacggttaca tgattctgga gtactt
//
LOCUS       DASYCU010000257         5307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4262413, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000257 DASYCU010000000
VERSION     DASYCU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262413"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGM24_05135"
     CDS             <1..237
                     /locus_tag="VGM24_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HEY4154783.1"
                     /translation="YPSGKVTKKEVPVRNGLKQEDAKKIVKLIKDSGLKVQAAIMDDL
                     IRVTGKKLDDLQAVIQRCKTANLEVPLQYDNMRN"
     gene            338..586
                     /locus_tag="VGM24_05140"
     CDS             338..586
                     /locus_tag="VGM24_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEY4154784.1"
                     /translation="MKENLHPENYRLVVFRDMSNGTSFLTRSAANSRETIKWEDGNEY
                     PLIKLEISNTSHPFFTGKNVLVDTAGRIDKFKKRYAKK"
     gene            705..1754
                     /locus_tag="VGM24_05145"
     CDS             705..1754
                     /locus_tag="VGM24_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEY4154785.1"
                     /translation="MQSMSLVDGPFADDLFPISLTRSVADIRCGILSLRQKWAYFFPP
                     AGSGKDSVPVPANILPDPDLVKAIRLGGETEIQLAISRASKLTNPLDILRQNDREIRL
                     DFERVTHGRPSGPIPASNRVLLPEQIFIEEGAVVEHSILNASGGPIYIGKNALVMEGS
                     MIRGPFALNEHSVLKMGAKIYGASTVGPYSTVGGEVKNSVILGYSSKAHDGYLGDSVI
                     GEWCNLGGGSSNSNISNSAGPAKLWNPLKNSFTDSSLKAGLFMGDYSRSAINTSFNTC
                     TVVGVSVNVFGKGLTPAYLPSFSWGFDPAETYEFEKAIQHIAQWKKLKNKTLSTAEIN
                     TLKDIFDRHDRQIRE"
     gene            1793..2557
                     /gene="tpiA"
                     /locus_tag="VGM24_05150"
     CDS             1793..2557
                     /gene="tpiA"
                     /locus_tag="VGM24_05150"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY4154786.1"
                     /translation="MRKQIAAANWKMNLTYEQGAQLLEAIVSADVSLNEKQLALFAVP
                     YPYLELASDTIHHKKYYFAAAQNCSDKKSGAFTGEVSAEMLKSVAVSYCLVGHSERRE
                     YFGETNEVLASKVSLCLTYDVTPVFCCGEALEIRERGNQNAFVEQQLKESLFHLSAEQ
                     LLKVIIAYEPIWAIGTGKTASTEQAQEMHAHIRSVLAKQFDDAVAQQISILYGGSVKA
                     NNARELFANPDVDGGLVGGASLVASDFIEIMRALKQ"
     gene            2625..4007
                     /locus_tag="VGM24_05155"
     CDS             2625..4007
                     /locus_tag="VGM24_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY4154787.1"
                     /translation="MDSWFSRIRQFLGKDNISFYENILLKRGFSVLLIQFGGLAIVFA
                     SNILLARLYGEQAYGIYSLVSSWCMLLAVLGLFGMDDSHLVQLPAWKLKGENKKIRQQ
                     LKWSLGINLISIFVITAVFYFILHVAKLPRLSDYAYYFNYGFVLVLFLAIMNNLISFL
                     RGMDKVIYGEIMDKMARPFFLIILLLVFYYLSKSDLVLDSLLAAAAGLFFILILLFFK
                     IQKTVREIKGTGHESKKNYSLGPNFRYVFLNLLYFLSTRMDLLLLTLFSDAVLVGHYN
                     VAVKFADVSSYPIAIINLSLPTLLSTVKHEKGKQAGPLLLYRVSKNSFFQCLLLSGLF
                     VLTGNWILHWYGKGFGDAYPILLVFLLSNLITAFIGSADIYFVLEGRENRPIYSRIAS
                     LMLTIALAFFLIPKWQLMGAAVSMLLGNLLYCALMEYFFFARHRFFIHPFISGNKSAM
                     GGKQEPESLG"
     gene            complement(4012..4908)
                     /locus_tag="VGM24_05160"
     CDS             complement(4012..4908)
                     /locus_tag="VGM24_05160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4154788.1"
                     /translation="MEYHLLGSSSLKISRIGFGCMSLDAGNPEADKVLQRAISLGINY
                     FDTADLYQHGLNEELLGRVFKGRRHQVILASKAGNQWRPDGSGWDWNPSKKYILACAE
                     QSLRRLQTSYIDLYQLHGGTIQDPIEETIEAFELLKAQGKIRYYGISSIRPNVIREYI
                     QRSGIASVMMQYSLLDRRPEESCFDLLKSRNIAVLARGPVAKGLLVNKPPEPYLNYSG
                     AEVQQAASALASLSGGKRSAAQTAIRFILKEEPVTSAVVGIRTMEQLEEAVMTFDSPG
                     LQQNEIELLEQVLPPNKYDQHR"
     gene            5002..>5307
                     /locus_tag="VGM24_05165"
     CDS             5002..>5307
                     /locus_tag="VGM24_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4154789.1"
                     /translation="MNHIRNFCIIAHIDHGKSTLADRLLQTTHTISDRDMMEQVLDDM
                     DLEREKGITIKSHAIQISYTHKNQDYVLNLIDTPGHVDFSYEVSRALAACEGALLLVD
                     "
BASE COUNT         1363 a         1296 c         1240 g         1408 t
ORIGIN      
        1 tatccgagcg gtaaagtgac aaaaaaagaa gtccccgtgc gcaacggcct caaacaggaa
       61 gacgccaaaa agatcgtgaa gctgataaaa gattcgggac tgaaggttca ggccgccatc
      121 atggacgacc tgatccgggt aaccggcaaa aaactggacg accttcaggc agtgatccaa
      181 cgctgcaaga cggctaatct ggaagtaccc ctgcaatacg ataatatgcg caattaatgg
      241 cttgaaaaat ttgtttccct gagtttcagc ccttaacttt gcgccccctt tcgtgccggg
      301 atcaccaatc attcatcgca cggctaaatt agaaaatatg aaagagaatc tgcatccgga
      361 aaattatcgc ctggtggtat tccgggacat gagcaatggg accagtttcc tgacgcgttc
      421 agcggccaat tccagggaaa ccattaaatg ggaggacggg aatgaatacc cgttgatcaa
      481 acttgaaatt tccaatactt cccatccgtt ttttaccgga aagaatgtgc tggtggatac
      541 cgccggtcgt atcgacaagt tcaagaaacg ttacgctaaa aaataaattt ccaccgtatg
      601 gaatgatccc tttctgcaaa acagaaaggg atttttttta ggcccttcga aaccggaaaa
      661 gatccggccc tccgccccaa ctcaaaaaaa acgtagtttt aaccatgcaa tcgatgtccc
      721 tcgttgacgg gccatttgcg gatgatcttt tccccatttc cctgacccgt tctgttgcgg
      781 atatccgttg cgggatacta agcctccggc agaaatgggc ttattttttt ccccctgccg
      841 gttccggtaa agattccgtt ccggttccgg ccaatatttt accggatccg gatttggtaa
      901 aagccatccg ccttggcggt gaaacggaaa ttcagcttgc catttccagg gcgtctaaac
      961 tgaccaatcc actggatatt ctccggcaga atgatcggga aatccgcctc gactttgaac
     1021 gggtaaccca tggccgcccg tccggaccca ttccggcatc taaccgggtc cttcttcctg
     1081 aacagatatt tattgaagaa ggggcggtag tggaacatag tatactaaat gcttccggag
     1141 gacctattta tattggaaag aacgccctgg tcatggaagg cagtatgatt cgcgggccct
     1201 ttgctttaaa cgaacattcg gtgctgaaaa tgggagcaaa aatctatggt gccagcacgg
     1261 ttggcccata cagtacagtg gggggagagg ttaagaattc ggtgatactg ggctattcca
     1321 gcaaagccca cgatggatac ctgggcgatt cggtgatcgg agaatggtgc aatctcggcg
     1381 gcggcagttc caattcaaat atcagtaata gcgcaggccc ggcaaaactg tggaatcctt
     1441 taaaaaactc ctttacagac agcagcctga aagccggatt attcatgggc gattattccc
     1501 gttccgccat taatacctct ttcaatacct gcacggtggt aggagtaagt gtgaatgttt
     1561 ttggaaaggg gctgacaccc gcttatcttc cctctttcag ttggggtttt gacccggcgg
     1621 aaacctatga atttgaaaag gccatccagc acattgccca atggaagaag ctgaagaaca
     1681 aaaccctttc aaccgctgaa ataaatacgc tgaaagatat atttgaccgg catgaccggc
     1741 aaatacgcga ataatcatat taaaataatt tttgatcaac aaataaacgg taatgagaaa
     1801 acaaatcgct gcggcaaact ggaaaatgaa tctaacctac gagcagggcg ctcagttatt
     1861 ggaagccatt gtatcggccg acgtatcact gaatgaaaaa caactggctc tcttcgcggt
     1921 cccttatcct tatcttgagc tggccagtga tacgattcat cataaaaaat attatttcgc
     1981 agcagcacag aactgttccg acaaaaaatc aggcgcgttt accggagagg tttctgccga
     2041 gatgttgaaa tccgttgctg tttcttattg cctggtggga cacagcgaac gacgcgaata
     2101 ttttggggaa accaacgagg tgctggcgag taaggtttcc ctttgtctga cttatgatgt
     2161 aacccccgtt ttttgctgcg gcgaagcgct ggaaatccgc gaacggggaa atcagaatgc
     2221 attcgtggaa caacaactga aagaatcgct gtttcatctg tcagcagaac aactattgaa
     2281 agtgataatt gcctatgagc cgatctgggc catcggcacg ggtaaaacgg ccagcacgga
     2341 acaggcccag gagatgcatg cccatatccg ctccgtttta gcgaaacaat ttgacgatgc
     2401 agtggcacag cagatttcca ttttatacgg agggagcgta aaagccaata atgccaggga
     2461 gttattcgcc aatcccgatg tggacggcgg tttggtgggt ggcgcttctc tggtggcatc
     2521 tgattttatc gagatcatgc gggctttaaa acaataaggt cattgcctgc gttacggcaa
     2581 attttccttt cggttggcgc tttatcatct cagtttattt tcccatggat tcatggttca
     2641 gcagaatacg gcagtttctt ggaaaagaca atatttcctt ttacgaaaac atattgctca
     2701 agagaggctt ttcggtactc ctgatccaat ttggaggact ggccattgtt tttgcttcca
     2761 atatattact ggcccggcta tacggcgaac aggcatatgg tatttattcc ttagtcagca
     2821 gctggtgtat gctcctggcg gtgctcggac tattcggaat ggacgattcc catttggtgc
     2881 aattgccggc atggaaactg aaaggggaga ataaaaaaat tagacaacaa ctgaaatggt
     2941 cgctgggaat caacctcatt tccattttcg tcataaccgc ggtattttat tttatcctgc
     3001 acgttgccaa actgcccagg ctctccgact atgcctacta tttcaactat gggttcgtcc
     3061 tggtgctgtt tctggcgatc atgaataatc tgatctcttt tctccggggc atggataaag
     3121 tgatctacgg tgaaatcatg gataaaatgg ctagaccttt cttccttatc attttattac
     3181 tggtcttcta ttatctatcg aaatccgatc tggtgctgga ttcccttctg gccgccgctg
     3241 ccggtctgtt tttcatcctc atcctccttt tttttaaaat acaaaagacc gtccgcgaaa
     3301 taaaggggac tggccatgaa tctaaaaaaa actattccct tgggcccaat ttccggtatg
     3361 tatttctgaa cctgctttat ttcctttcca cccgcatgga tctcctcctg ttaaccctgt
     3421 tttcagacgc ggtattggtc gggcattaca atgtggccgt taagtttgcg gatgtatctt
     3481 cctatccgat tgccatcatc aatctttccc tgccaaccct gctctccacc gtaaagcatg
     3541 aaaagggtaa gcaggccggc cccctgctgc tataccgggt ttcgaaaaac agtttcttcc
     3601 aatgtcttct gctcagcggt ttatttgtgc taacgggcaa ttggatatta cactggtacg
     3661 ggaaaggatt cggggacgct taccccatac tgcttgtttt tcttctatcg aacctgatta
     3721 ccgcatttat cggttcagca gatatttatt ttgttttgga gggacgggaa aacaggccca
     3781 tctacagccg gatcgcctct ttaatgctga ccatcgcgct tgcgtttttc ctgatcccaa
     3841 aatggcagtt gatgggcgca gcggtttcca tgctgctggg caatctcctg tattgcgctt
     3901 tgatggagta ttttttcttc gccaggcacc ggttcttcat ccaccctttc atatcaggga
     3961 acaaatcggc catgggtggc aaacaagaac cggagtcttt gggatgatat cttatctgtg
     4021 ctggtcatat ttattgggcg gcaaaacctg ctccaataat tcgatttcat tctgttgtaa
     4081 cccgggcgaa tcaaaggtca ttaccgcttc ttccagttgt tccatcgttc tgatccccac
     4141 cacagcagac gtcaccggtt cttctttcaa tataaagcga atagccgttt gtgcggcact
     4201 tcgctttccg ccagacagcg aagcaagggc agacgcagcc tgctgcactt ccgcgccgga
     4261 ataattcaga taaggttcag gaggtttatt taccagcagc ccctttgcca ccggtccccg
     4321 tgccagcaca gcaatgttcc tgcttttcaa aagatcaaaa caggattctt cggggcggcg
     4381 gtcaagcaaa ctgtattgca tcattacgct tgcgataccg gaacgctgaa tgtattcgcg
     4441 gatcacattg ggccggatgg aggagatgcc gtaataccgg atctttccct gtgcttttag
     4501 cagttcgaat gcttctatgg tttcctctat cggatcctgg atggttcccc catgcagctg
     4561 gtagaggtct atataactgg tctgcagacg gcgcaggctt tgctcagcac aggccaggat
     4621 atattttttc gaagggttcc aatcccagcc gctcccatcc ggacgccatt gattaccggc
     4681 tttactggcc aggatcacct ggtgccgcct gcctttgaaa acccttccca gcaattcctc
     4741 attgagaccg tgctggtata agtccgcggt atcaaaataa ttaatgccca gggaaatggc
     4801 ccgctgaagg accttatccg cttcgggatt gcctgcatcg agtgacatac agccgaatcc
     4861 gatccggctg attttcaatg aagagctccc caataaatga tattccataa aaatggcgcc
     4921 ccggtttgta tgaacccggc aagttccgaa ttattttgaa gataaaaatt ggtcagccaa
     4981 tttcgttacc ttcgcgcacc catgaatcat atccgaaatt tttgcattat cgcgcatatc
     5041 gaccacggaa aatccaccct ggccgaccgg cttctacaaa ccacccatac catcagcgac
     5101 cgggatatga tggagcaggt gctggacgat atggacctgg aaagagaaaa aggcattacg
     5161 ataaagagcc atgccattca gatcagttat acccataaaa atcaggacta tgtgctgaac
     5221 ctgatcgata cgcccggcca tgtggacttc agctatgaag tcagtcgtgc cctcgccgcc
     5281 tgcgaaggag cgttattact cgtggac
//
LOCUS       DASYCU010000258         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2812331, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000258 DASYCU010000000
VERSION     DASYCU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2812331"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VGM24_05170"
     CDS             <1..832
                     /locus_tag="VGM24_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154790.1"
                     /translation="PDGTSAYTGDPTFDAIALKSGDIDKQTFMITHLEYVFDNTIVKT
                     TNIMNGLRYKFYVDFNYQVNSPQQTGEGRKMFNFGFDARNYYPIYRNFIWALRAAGDF
                     SWGNQKIIYYLGGMDGELFPKANQYPSPDYSQSYAYQSLALNLRGFKQNVANGNNAIT
                     VNSEFRLPVFTTLFNKPINNAFLRNFELTQFIDLGTAWSGSLKNIERPSMHYPNGDPS
                     VPVDVILKAGGIGPFAASYGFGARTTLLGYFLRVDAGWEMKGLFRGGPMWQFAMGVDF
                     "
     gene            complement(853..1188)
                     /locus_tag="VGM24_05175"
     CDS             complement(853..1188)
                     /locus_tag="VGM24_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEY4154791.1"
                     /translation="MPKKNIPDKNGLVYSTDPDFSFAGETTPGKETLSPGKQRLRIVL
                     DTRHRAGKTVTAILGFVGKTEDLETLGKKIKTHCGSGGSVKDGEILVQGDQLLKVKNW
                     LLKEGYRVI"
     gene            complement(1231..>1885)
                     /gene="nadC"
                     /locus_tag="VGM24_05180"
     CDS             complement(1231..>1885)
                     /gene="nadC"
                     /locus_tag="VGM24_05180"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY4154792.1"
                     /translation="FHLHKKDGDPMQPGETAFELEGSVHTILICERLLLNCMQRMSGI
                     ATLTRQYAEKIRDYSSKILDTRKTTPNFRLLEKEAVGIGGGHNHRFGLFDMIMLKDNH
                     IDYCGGIGKAIERAYRYVQEVKPGLKIEVEARSLEDVREVLKTGKADRILLDNFNPGQ
                     LKEAMDLIQQKLETEASGGINLENIRSYAATGVDFISIGALIHQAKSVDLSLKAVLY"
BASE COUNT          432 a          493 c          443 g          517 t
ORIGIN      
        1 accggacgga acaagtgcct ataccggaga cccgacgttt gatgcgattg cgctgaaatc
       61 cggggacata gacaagcaga ccttcatgat aacgcacctg gaatacgttt tcgacaatac
      121 gattgtgaaa acaaccaata tcatgaatgg gttgcgctat aaattctacg tggatttcaa
      181 ttaccaggtt aattccccgc agcaaaccgg ggagggcaga aaaatgttca atttcggatt
      241 cgatgcaaga aattactatc cgatctaccg gaatttcatc tgggcattgc gcgccgccgg
      301 cgatttctcc tgggggaatc agaagatcat ctattattta ggcggcatgg acggagagct
      361 tttcccgaaa gccaatcagt acccgagccc ggattattcc cagagctatg cctaccagtc
      421 cctcgccctt aatctgcgcg gctttaagca gaatgtggcc aacggaaata atgccattac
      481 ggtgaacagt gaattccgct tgccggtatt taccaccttg ttcaataagc cgatcaacaa
      541 tgcttttctc cgcaattttg aactgaccca gttcatcgat ctgggcacgg cctggtccgg
      601 ctcgctaaag aatattgaac gcccttccat gcattatccg aatggcgatc cttccgttcc
      661 cgtcgacgtg attttgaaag cgggcggaat cggtcctttt gccgcgagtt atggatttgg
      721 cgcaagaacc acgcttctcg gatatttcct gagagtggat gccggttggg aaatgaaagg
      781 gcttttccgg ggtggcccga tgtggcagtt tgccatggga gtggattttt gatgggtttg
      841 aaaatacgct gatcagatta cccggtaccc ttctttcagc aaccagttct tcacttttag
      901 caactggtct ccctgtacaa ggatttctcc gtcttttaca gacccgccgc tgccgcaatg
      961 ggtctttatt ttcttaccca gcgtttccag gtcttccgtt ttcccaacaa agcccaggat
     1021 ggcggttacc gtttttccgg cgcggtgccg ggtatccaat acgatgcgca aacgctgttt
     1081 gcccggagac agcgtctctt tgcccggagt cgtttcaccg gcaaatgaaa aatccggatc
     1141 cgtgctgtat accaatccgt ttttgtcggg gatattcttt tttggcataa gatctaattg
     1201 ataccggaag aggagattgg gtattctccg ctaatataaa accgccttca aactcaaatc
     1261 gacacttttt gcctggtgaa tcagcgcacc gatactgata aaatccacgc cggttgccgc
     1321 ataggaacgg atattctcca gattgatccc cccgcttgct tctgtttcca gtttctgctg
     1381 aatcaaatcc atcgcttcct ttaactgtcc gggattgaaa ttatccagca agatccggtc
     1441 tgccttaccc gtttttaata cttcccgcac atcctccagg cttcgggctt ccacttctat
     1501 tttcaagccg ggtttaacct cctggacata acggtaggcc cgttcaatgg ctttcccgat
     1561 gcccccgcaa taatcaatat ggttatcctt cagcatgatc atgtcaaata acccgaaccg
     1621 gtgattatgc ccgcctccga tccctacagc ctctttctcg aggaggcgga aattcggtgt
     1681 ggtttttctg gtatccaata ttttggatga atagtccctg attttttccg catactgacg
     1741 ggtcagtgtg gcgatcccgc tcatgcgctg catgcagttc agcaggaggc gttcgcagat
     1801 caggatggta tggacggagc cctccagctc aaatgccgtt tccccgggct gcatgggatc
     1861 gccatccttt ttatgcagat gaaat
//
LOCUS       DASYCU010000259         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2521955, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000259 DASYCU010000000
VERSION     DASYCU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521955"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGM24_05185"
     CDS             <1..361
                     /locus_tag="VGM24_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154793.1"
                     /translation="DGAGGFLPLSILQSGIYIPGNGKALVKLQGSSGKYLVAASQNKD
                     VLKLYELKRKTNSLRIEPDEVSALIRLKDGKMQKEEFYYGTSFLSQSARFLNLDSNMV
                     NIKITNSKGKIRTVNLP"
     gene            complement(348..1634)
                     /locus_tag="VGM24_05190"
     CDS             complement(348..1634)
                     /locus_tag="VGM24_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY4154794.1"
                     /translation="MIKHLLIKIEGFPRSFLIALGISLAVLLFTQCVHNDKDEKRVDQ
                     QPAFGQFAGDQVCASCHKNIGNSFIHTAHYLTSAPASGKTIRGSFAACKNIFTYDSVR
                     RVVMEKRDSGFYQVLYDQGVEKTARRFDITIGSGTRGQTYLTWIGNTLYQLPVSYFTL
                     TDSWSNSPGYLPHKINFDRPIASRCLECHSTFANTLSAPDAVPEKFDPEQVLFGVGCE
                     KCHGPAANHVAFHTQNPKETKAKFIIDPTSLSRQQSLDLCALCHGGRLGKLRPSFQFT
                     VGDSLSNYFLVDTNRPDPSGIDVHGNQYGLLRSSKCFRISGTMTCVTCHDPHANQRGQ
                     LALFSERCMGCHNTEHGNSCKMEEKLGSAIVQNCIDCHMPRRPSRLISVLLPGNIRPT
                     AATIRSHFISIYPEETKKYLGGRNSSGKNHSSAMAN"
     gene            1763..1846
                     /locus_tag="VGM24_05195"
     tRNA            1763..1846
                     /locus_tag="VGM24_05195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1797..1799,aa:Leu,seq:caa)
     gene            2053..2463
                     /locus_tag="VGM24_05200"
     CDS             2053..2463
                     /locus_tag="VGM24_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4154795.1"
                     /translation="MMAKHAHILLADDDVEDQDILKSIFTEKDLNVSIHCVFNGKQAI
                     AYLEGCSPRELPSLIILDYKMPVMNAVETLERLKDNARLKGIPKVVWSTSEQNEHRKL
                     SMENGAIQYFAKPNNQAELSDLPKQMLLILNNQN"
     gene            complement(2479..>3564)
                     /locus_tag="VGM24_05205"
     CDS             complement(2479..>3564)
                     /locus_tag="VGM24_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4154796.1"
                     /translation="VEDYAILLLDRDGIIMNWNKGAQKIKGYREDEIIGKSFRVFYLP
                     EDRQRKLPEKLIDEASRNGKATHEGWRLRKDGTRFWGSIVITSLHDDQHNIIGFTKVT
                     RDLTEFKIASDQIKDYARELEFQNKELQQFAYAAAHDLKEPLRKVQFYNSAILENAGE
                     ALPDRERTFLNNSINSVKRMKGLIDDLLKYAKVSEYLEKFEDVDIKTIVEEIMVFYRD
                     LSGDDIIRAELNALPVISGIPFQIRQLFENLLGNAFKYHHRDRKPVIQITCEKEFHTF
                     VTESDTDSTAPYYKITVQDNGIGFEPEFAQKIFELFHRLHPREEYSGTGVGLAICKRI
                     VQNHKGFIEAASLPDQGSAFTIYLPCS"
BASE COUNT          941 a          830 c          806 g          987 t
ORIGIN      
        1 tgacggagcc ggaggctttc taccgctttc tatccttcag agcgggatct acatccccgg
       61 gaacggtaag gccctggtaa agctgcaggg cagttccggc aagtacctgg tggccgccag
      121 ccaaaacaag gatgtcttaa aattatatga actgaaaaga aaaacgaaca gccttcgaat
      181 tgaaccggac gaggtgagcg ccctgatcag gttaaaagac ggtaaaatgc aaaaggaaga
      241 gttttattac ggaacttctt ttttatcaca atctgcgagg ttcctgaatc tggacagcaa
      301 tatggtgaat atcaaaatca cgaacagtaa aggtaaaatc cggaccgtta atttgccata
      361 gccgaagaat ggtttttccc ggagctattt ctccccccga ggtatttttt tgtttcctcc
      421 ggataaatgc taatgaaatg agaccgtatc gtagcggccg ttggccggat atttcccggc
      481 agcaatacgg aaatcagccg ggaaggcctc ctcggcatat gacaatcgat acagttttga
      541 acaattgcgg atccgagctt ctcttccatt ttacaggaat tgccgtgttc ggtattgtgg
      601 caacccatgc agcgttcgga aaacagggca agctgtcccc tttgatttgc gtgagggtca
      661 tggcaggtga cacaggtcat tgttccgctt atcctaaaac atttggagga gcggagcaat
      721 ccatactggt tgccatgcac atcaattccg gagggatcag gacggttggt gtcaaccaaa
      781 aaataattgg aaagactatc cccaaccgta aattgaaaag agggcctgag cttgcctaat
      841 cttcctccgt gacataaagc gcacagatca aggctttgct gccgggacag tgaagtggga
      901 tcaataataa attttgcctt tgtttctttc gggttttggg tatgaaaagc aacgtggttt
      961 gctgccgggc catggcattt ttcacaaccc accccaaaca gaacctgctc cgggtcaaat
     1021 ttttcaggca ccgcatccgg agcagaaagt gtattcgcaa acgtgctgtg gcattccaga
     1081 cagcgggagg cgatcggccg gtcaaaatta attttgtggg gaagataacc cgggctgttc
     1141 gaccagctat ccgtgagtgt aaaatacgaa accggcaact ggtaaagggt gtttccaatc
     1201 caggtcaaat aggtctgccc tctggtaccc gagccaatcg taatgtcaaa acgcctcgcc
     1261 gttttttcaa ctccctggtc gtacaggacc tggtaaaatc cgctatccct tttttccatg
     1321 accacccttc tgacggaatc ataagtgaat atgtttttac aggcggcaaa acttcccctg
     1381 atcgtttttc cggaagccgg tgcggaggta agataatgcg cggtatggat aaaactattc
     1441 cctatattct tatggcagct ggcgcatacc tggtctccgg caaactgacc gaaggcgggt
     1501 tgctgatcaa cccgcttttc atccttatcg ttgtgcacgc attgcgtaaa taataaaacg
     1561 gcgaggctaa tacccagggc aatcaaaaat gagcggggaa atccttcgat ctttatcagg
     1621 aggtgtttaa tcattcaaaa tcggttcgca tatattggct gataaagtta attcatgaca
     1681 aaggatcccg tccaccgtca tttcttttta cacttatgtc ctaaacaatt acatttgcag
     1741 cccatttctc tgtttcccaa ttgcccggat ggcggaattg gtagacgcgt cagactcaaa
     1801 atctggtatt cgcaaggatg tgcaggttcg attcctgttc cgggcacggc aaactccttt
     1861 aaaatcaatg atttttaagg agttttgcat tttttattcc cattaaatta aagtcttttt
     1921 ttggcttatt cctattattt ttggggagac ggatagattt cgggccgtcc ggtccggatc
     1981 cttatgccga acaggtaaga acatttaaaa aggtttattt aaaggcattt aatctttccc
     2041 aaccaaaaca aaatgatggc aaagcacgcg cacattcttt tggcagatga cgatgttgaa
     2101 gaccaggata ttctaaaatc catttttaca gagaaagatc tgaatgtttc catccattgt
     2161 gtcttcaatg gcaaacaggc cattgcctat ctggaaggtt gttctcccag ggaactacct
     2221 tccctgatta tactggatta taagatgccg gtcatgaatg cggttgaaac gctggaaagg
     2281 cttaaggaca atgcccgcct taagggaatt cctaaagtgg tatggagcac atcggaacaa
     2341 aatgagcacc ggaaactaag tatggaaaac ggtgccattc aatatttcgc aaaaccaaac
     2401 aatcaggccg aactttccga tctgcctaaa caaatgctgc ttattctcaa taatcaaaac
     2461 taaatcagga gtattcgttc aggagcaggg caggtatatg gtaaaggccg atccctgatc
     2521 cggcaggctg gccgcttcaa taaatccctt gtgattttgg acgattcttt tgcaaatggc
     2581 caaccccaca ccagtgccgg aatactcctc ccgggggtga agccggtgga ataattcgaa
     2641 aatcttttgg gcaaattccg gttcaaatcc gataccattg tcctgaacgg taattttgta
     2701 gtatggagcg gttgaatcgg tatccgactc ggtaacaaac gtatgaaatt ctttttcgca
     2761 ggtgatctga attacgggtt tgcggtcccg gtggtggtat ttaaatgcat ttcccagaag
     2821 attttcaaat aattgcctga tctgaaaagg tattcccgag atgaccggca aagcattcaa
     2881 ttctgccctg attatgtcat cgcccgacag atcccggtaa aagaccataa tctcttccac
     2941 tattgttttt atatccacgt cctcgaattt ttccaaatac tcggagactt tggcgtactt
     3001 cagcaggtca tcaatcaatc ctttcatccg ctttaccgaa ttaatcgaat tatttaagaa
     3061 ggttctttcc cggtccggca gggcttcccc ggcattttcc agaatagccg aattataaaa
     3121 ttgtactttt ctcaacggtt ctttcagatc atgggcagcg gcgtacgcga attgctgtaa
     3181 ttccttgttc tgaaattcca attcacgcgc gtagtcttta atctgatcgc tggcgatctt
     3241 gaattccgtc agatcgcgtg tcactttcgt gaatccgata atgttgtgct ggtcgtcgtg
     3301 caagctcgta atgacgatgc ttccccagaa acgggtgcca tccttgcgca acctccatcc
     3361 ttcatgggtc gccttgccat tccgggaggc ttcgtcaatt aatttttccg gcaatttcct
     3421 ttgccggtct tcggggaggt aaaaaacccg gaaactcttt ccaataattt catcctcccg
     3481 gtatcccttt attttttggg cccctttgtt ccagttcatg atgatcccat cccggtccag
     3541 taaaaggatc gcatagtcct ccac
//
LOCUS       DASYCU010000260         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_58169, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000260 DASYCU010000000
VERSION     DASYCU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58169"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..795
                     /locus_tag="VGM24_05210"
     CDS             172..795
                     /locus_tag="VGM24_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154797.1"
                     /translation="MKWIFIFLLFSIASCKRGPVCFVTSADIQKQSPQKLLVSYGSGK
                     KIAGLYDSGWDSTKGGVYLFYPNDHLKSYTFYQNNKKAVYREEYNENGVMVHSEGSPM
                     VDRIITEINLDSAYFQIYFFKLQKTFQRLQITINQNPALQFTLQNDSLYSNIKMVSFG
                     LNTKDLSKLNIYSRLEYLNDCSKIDHILTDTLLLVKNPHISPANAGK"
     gene            835..1791
                     /locus_tag="VGM24_05215"
     CDS             835..1791
                     /locus_tag="VGM24_05215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4154798.1"
                     /translation="MKVIFIKNILHSIFLFAFLFAGNSGLFSQRIVDITYRQDARGAY
                     VFSGINHGFCNYILEIHFTVFDNLKADREMPYRAEIKPGTNKLFSLLAVKPDVPVKFN
                     YSSTYHKGCIHPAVDSDFTYLFPITPGKEAQVYEMSSPDKPAAEGISDNNWYVIRLKM
                     KPGDTIYAARKGVVTEVEDRDGSNDAGSGSPGKENYIELVHADCSFGRYGILKKNGAL
                     VKPGQTVKAGQPIGLVGGDQYGRGSDIRFSVYYYQEEEVSRNGELLWKGFRNYIHLQC
                     WTKNNGKGRLKHGAVYRSEFPLAVLNQESEKKSQKPRMKAKK"
     gene            1867..3678
                     /locus_tag="VGM24_05220"
     CDS             1867..3678
                     /locus_tag="VGM24_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY4154799.1"
                     /translation="MAAGLMSSSLFSQADLITNIDGRNSFSLDGQWQYIVDPYETGIY
                     DYRHKELPQNNPSAYWNPDQPASKMDRKEYGYTEQSSLKVPGDWNSQRPEFLYYEGTV
                     WYKKSFDFRKSKSSGKLFLYFAAVNYRADVYLNGRKLGIHEGGFTPFDFEIPDSLIKE
                     RGNFLVVRVDNKRSAEGVPTLNTDWWNYGGITRSVKIIELPPAAIRDYFIQLKPKSGS
                     SGMVEGWVKLDSLIAGDKISIEIPALRLLKIFPVKDKKVPVHFTLPSFEPWSPEKPVC
                     YRVIIRNGTDRVEEKIGFRTIETKDRKILLNGKPVFLRGVCIHEEIPQQGRRAYSRED
                     ALQLLGWAKELNCNMVRLAHYPHDESMTRAADSLGILVWSEIPVYWTIDFSDSNVLKN
                     AERQLTEEITRDHNRSAVIIWSVGNETPVSETRTAFMKALVDSARILDPDRLLTAALE
                     VNYRSGKEQSRMVDDPLGEYLDLISFNEYLGWYGGTPESCRTASWGTKYNKPLFISET
                     GAGALGGFHADTLTVWSEEYQAWYFKEQTQMMERMPDGFSGVSPWILADFRSPRRNNP
                     VYQQGWNRKGLIGQDGNKKQAFFILKHYYDVMEKAHK"
BASE COUNT         1084 a          809 c          977 g          966 t
ORIGIN      
        1 atcaacggag gcggaattcc gatcatcgcc gatgccggag gcggccgggt tgacctgtcc
       61 ttcaaatgag ctgaaccgca gccctgaatt tatttttaaa tagcttgcag gcacatcgcc
      121 ggtaagctat tttagtatat atctttaggg ataagtcaac ggttgagtca tatgaaatgg
      181 atatttattt tccttttgtt ttctatagcc tcctgtaaac ggggacctgt atgttttgta
      241 acctcagcgg atattcaaaa gcagtccccg caaaaattat tggtttccta tggctccggc
      301 aaaaaaattg ccggcctgta tgattccggg tgggacagca ccaagggcgg tgtttactta
      361 ttttatccca atgaccatct gaagtcttac accttctatc aaaacaataa aaaggcggtg
      421 taccgggaag aatacaatga aaacggcgta atggtccatt ccgaaggaag cccgatggtt
      481 gaccggatca ttacggaaat caatctggac tccgcctatt ttcaaattta tttttttaaa
      541 ctccagaaaa catttcagcg cctgcaaatc acaataaatc agaatccggc tttacaattt
      601 accctgcaga atgattcgtt gtactccaat atcaaaatgg tttcttttgg attaaataca
      661 aaagatctga gcaaactgaa tatctattcc cggttggaat acctgaatga ctgttccaaa
      721 atagatcata ttctgaccga cacgctttta ctcgtaaaga atccgcatat ttctccggca
      781 aatgcgggta aatagagtat cccgggggta aatggagtta aaaacttatt tttggtgaaa
      841 gtaattttta tcaaaaacat tcttcattcc atctttctat ttgcattcct ttttgccggg
      901 aattccggat tattctccca acggatagtg gatatcacat accggcagga tgcccgggga
      961 gcttacgtat tttcgggaat aaatcacggt ttctgcaact atattctgga aatccatttt
     1021 accgtctttg acaatttaaa agctgatcgc gaaatgcctt accgggcgga aataaagccc
     1081 gggacgaata aattgttttc ccttcttgcg gtaaagccgg atgttccggt gaaattcaat
     1141 tattccagca cctatcacaa gggatgcatc catccggcag tggactcgga ttttacttat
     1201 ctctttccca ttaccccagg gaaagaagcc caggtttatg agatgagcag cccggataaa
     1261 ccggctgcag aaggtatttc cgacaataac tggtatgtga tccggttaaa aatgaaaccg
     1321 ggagacacga tttatgccgc caggaaggga gtggtcacgg aagtggaaga ccgggacgga
     1381 agcaacgatg caggcagcgg cagcccgggc aaggagaact atattgaact ggttcatgcc
     1441 gattgttcct tcggacgtta tggaattttg aaaaaaaacg gtgcattggt aaaaccggga
     1501 caaacagtaa aagccggaca acccattggt ctcgtcgggg gcgatcaata cggccgggga
     1561 tccgacatcc ggttcagtgt ttattattac caggaagaag aggtttcccg gaacggcgaa
     1621 ttgctctgga aaggattcag gaactatatt caccttcagt gctggacaaa aaataacggt
     1681 aaaggaagat tgaaacacgg ggctgtttac cggagcgaat ttcccttggc ggtgctgaac
     1741 caggaatcgg agaaaaaaag ccaaaagccg agaatgaaag ctaagaaata aggatttact
     1801 aaacgatttt ttcaattaaa cggctgttgt atgaaaccgc ttttgagggc aaaatttatt
     1861 tgttttatgg ctgcgggttt aatgagttcg agcctgtttt cccaagcgga tttgattacc
     1921 aatatcgacg ggcggaacag ttttagcctg gacggtcaat ggcaatatat cgttgatccc
     1981 tatgaaaccg gcatttatga ttaccgccac aaggaactcc cgcagaacaa tccgtcggcc
     2041 tactggaatc cggatcagcc tgccagtaaa atggaccgca aggaatatgg atatacggaa
     2101 caatccagcc tgaaggttcc cggtgactgg aattcgcagc ggccggaatt tttatattat
     2161 gaaggtacgg tctggtacaa gaaatcgttt gatttccgga aatcaaaatc atccggtaag
     2221 ctctttcttt attttgcagc cgttaactac cgggcagatg tttatctcaa tgggagaaag
     2281 ctggggatac atgaaggcgg tttcacgccc tttgattttg aaatcccgga ttccctgata
     2341 aaagagaggg gaaactttct ggtcgtccgg gtagacaata aacggtctgc cgaaggagta
     2401 cccaccttaa acacagactg gtggaactat ggcggcataa cccgaagtgt aaagatcatc
     2461 gaattgcctc cggcggccat tcgcgattat tttatccagt taaaaccaaa gagcgggtct
     2521 tccggaatgg tggagggctg ggttaagctg gatagtctta ttgccgggga taaaataagt
     2581 atcgagatcc cggcattaag gcttctgaaa atatttccgg tgaaggataa gaaagttccg
     2641 gttcatttta cgctgccttc ctttgaaccc tggtcgcctg aaaagccggt ttgttaccgg
     2701 gtgatcatcc ggaacggaac agaccgggtg gaagagaaaa tcggattccg gacaatagag
     2761 acgaaggata gaaaaatcct gctcaacggg aaaccggttt ttttgcgcgg ggtttgtatt
     2821 catgaagaaa ttccccagca gggccggcgg gcttacagcc gggaagatgc cttgcagtta
     2881 ttgggctggg ccaaagaact gaattgtaat atggtgaggc tggcacatta tccccacgac
     2941 gaaagcatga cccgggcggc agattcattg ggtatcctgg tatggtctga aattccggtt
     3001 tactggacca tcgatttttc cgacagtaac gtgttgaaaa atgcagaaag acagctcacg
     3061 gaagaaatta cgcgtgatca taaccggtct gccgtcataa tctggtctgt cgggaatgaa
     3121 acaccggtaa gcgaaacgag aactgctttt atgaaagcgc tggtggattc agccaggatc
     3181 ctcgatccgg accgtttgct gacggcggca ctggaagtaa attatcgcag tggaaaggag
     3241 cagtcccgga tggttgatga tccgcttggc gaatatctcg acctcatatc tttcaacgaa
     3301 tacctgggct ggtatggcgg aacgccggaa agctgcagaa ccgccagctg gggaacgaaa
     3361 tacaacaagc cgctttttat cagtgaaacg ggggcgggag cgctgggagg attccatgca
     3421 gatacgctga cggtctggag tgaggaatac caggcctggt attttaagga acaaacgcaa
     3481 atgatggaaa gaatgccgga cggtttttcc ggggtatctc cctggatact ggcagacttc
     3541 cgttcaccgc gccgcaataa cccggtttat cagcaaggct ggaacaggaa gggactgatc
     3601 ggccaggacg gaaataaaaa gcaggccttt tttattttga agcactacta tgatgtcatg
     3661 gagaaagcgc ataaatgagc atccgcggat tttgagttaa acccgtttta tttgaaggac
     3721 atgcaaacaa gtgccgatgt aattattatt ggagccggac tggccggatt ggtggctgcg
     3781 gcggaactgg tgcgggccgg gaagaaactc atcctggttg atcaggagcc tgaaca
//
LOCUS       DASYCU010000261         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5132865, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000261 DASYCU010000000
VERSION     DASYCU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5132865"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VGM24_05225"
     CDS             <1..534
                     /locus_tag="VGM24_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154800.1"
                     /translation="NRKNSINPDGGFYADILYRPNLKAMGSDTNWQSLTLDIRKYFRL
                     SPNNRNILAFWSYDWLTLSGKPPYLDLPATGWDTYNNTGRGYIQGRFRSENMLYLESE
                     FRFAISRNGLFGGVAFANMQSFSDYPSKKFSKLWPAAGGGLRIKVNKHSNTNIAIDYG
                     VGIDGSKGFFVNLGEVF"
     gene            complement(537..1682)
                     /gene="galK"
                     /locus_tag="VGM24_05230"
     CDS             complement(537..1682)
                     /gene="galK"
                     /locus_tag="VGM24_05230"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY4154801.1"
                     /translation="MEKQIRRQFQQYFGKDILIAAAPGRVNLIGEHTDYNEGFVLPGA
                     VDRNIYVGVDSNTNRILNVYAEQYQEKFTFPIDDVHPVKGWPTYLLGMIYHLLPHKQL
                     SVGMDVVVSGNVPVGAGMSSSAALCSAFGVALNEIFRLGLSRMEIALAAQKTEHEFAG
                     LKCGIMDMFASLHGKEGHVMKLDCRNLEYEYIPFDFPDYCIVLVNSMVSHSLSSSAYN
                     ERRQQCEDGVSYLKRFYPEIHSLRDVKPETLKQHRNEIDPVVFKRCLYVVEENQRLLE
                     GCDFLEAGRLDDFGKLMYATHEGLSRDYAVSCAESDFLVNAARSFPEVKGARQMGGGF
                     GGCIISLVKKDEADHFIKGIQEKYENRYGKTPDVFIMNIAEGAHLLS"
     gene            1708..>2284
                     /locus_tag="VGM24_05235"
     CDS             1708..>2284
                     /locus_tag="VGM24_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEY4154802.1"
                     /translation="MPFTISKTSAQDLNLLQLTDTSTGIRVKVLPDHGALLHEFSIPL
                     PGGHLQAVAGYKNLSDLRLNHPLFYRSAKLSPFPCRIYKGQNRFEEKAYEFKNKFIDG
                     SAIHGLLSDKPFVLVQDNADDFSSSVLLEYTYLHEEPGYPFDYKISVEYTLKKEGLLS
                     LKTTATNLSSGAIPIADGWHPYFSLGGKIDDW"
BASE COUNT          594 a          601 c          493 g          596 t
ORIGIN      
        1 aaccggaaaa attcaatcaa tccggatggc ggattttatg cagacattct ctaccgcccc
       61 aacctcaaag cgatgggaag cgataccaac tggcaatctc ttaccctgga catccggaaa
      121 tattttcgtc tttcgccaaa caaccggaat attcttgctt tctggagcta tgattggctc
      181 acgctctccg ggaaacctcc ttacctggat ttgccggcga cgggttggga tacttataac
      241 aatacaggca gggggtatat ccagggcagg ttccgttcgg agaatatgct gtatctggaa
      301 tcggaattcc gctttgccat cagcaggaac ggcctattcg gcggagtggc ttttgccaat
      361 atgcaaagct tttccgatta tccttcaaaa aaattcagta agctctggcc tgcagccgga
      421 ggcggccttc gcattaaggt gaataaacat tccaatacca atatcgcgat tgattatggg
      481 gtcggcatcg acgggtccaa aggctttttt gtaaatctgg gagaagtttt ctgagattag
      541 ctgagcaggt gtgctccttc agcaatattc atgataaaaa catccggtgt tttaccatac
      601 cggttttcat atttttcctg aatgccttta ataaaatgat ctgcctcgtc ttttttcacc
      661 aggctgatga tgcagccgcc aaatccgccc cccatttgcc ttgcgccctt cacttcagga
      721 aatgatctgg ccgcattcac cagaaaatcc gattccgcac agcttaccgc atagtccctg
      781 cttaaaccct cgtgggtggc atacatgagt ttgccaaaat catccagtcg tcccgcctcc
      841 agaaagtcgc aaccctccag caaacgctgg ttttcttcca ctacataaag acatcttttg
      901 aaaacgacgg gatctatttc gttccggtgt tgtttcagtg tttccggttt tacatccctc
      961 agggaatgga tctccgggta aaagcgtttc agataactga cgccatcctc gcactgctga
     1021 cgcctttcat tgtaagccga tgatgacagg gaatggctga ccatggaatt caccagtacg
     1081 atgcaataat ccggaaaatc aaatgggata tattcatatt cgaggttgcg gcaatccagc
     1141 ttcataacat gtccctcttt accatgcagg ctggcaaaca tgtccataat gccgcatttc
     1201 aggccggcaa attcatgctc ggttttttgg gctgcaagcg caatttccat tctggataga
     1261 cccaaacgaa aaatttcatt cagggcgacg ccaaaagccg aacacagcgc cgcggaagaa
     1321 ctcatgcccg cacccaccgg cacattcccg gatacaacca catccatgcc cacggagagt
     1381 tgcttgtgcg gaagcagatg ataaatcatt ccaagcaggt aggttggcca gcctttcacc
     1441 ggatgcacat catctatcgg gaaggtgaat ttttcctggt attgttcagc ataaacattc
     1501 agtatcctgt tcgtattgga gtcgacgccc acatagatat tccggtcaac ggcacccgga
     1561 agtacgaagc cttcgttata atccgtatgt tcaccgatta ggttgacccg tcccggtgcg
     1621 gcagcaatca gtatgtcctt tccgaaatat tgctgaaatt gcctccgaat ctgtttttcc
     1681 atcagggaaa ttatcaattt ttaattaatg ccatttacca tatccaaaac aagcgcacag
     1741 gatctgaacc tcctgcagtt gacagacaca tccaccggca tccgggtaaa ggtactaccg
     1801 gatcacgggg ctttgctcca cgaattttcc atacccctgc ccggagggca tttgcaggca
     1861 gtagccggtt ataaaaatct ttccgacctc cgcctgaatc atccgctttt ttaccggagc
     1921 gcaaaacttt cccccttccc ctgccgcatt tacaaagggc agaaccggtt tgaagaaaag
     1981 gcctatgaat tcaaaaataa attcatcgac ggatccgcca tccacggact gctctccgat
     2041 aaaccttttg ttctggtgca ggacaacgcg gacgattttt cttcatcggt ccttctcgaa
     2101 tacacttacc tgcacgaaga accgggatat cccttcgatt ataaaataag tgtcgaatat
     2161 acattaaaaa aggaaggctt gctttccctg aaaacgactg caaccaatct atcatccggg
     2221 gccatcccca ttgccgatgg ctggcatccc tatttcagtc tgggaggaaa gatagacgac
     2281 tggt
//
LOCUS       DASYCU010000262         5167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4408839, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000262 DASYCU010000000
VERSION     DASYCU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4408839"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGM24_05240"
     CDS             complement(<1..187)
                     /locus_tag="VGM24_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154803.1"
                     /translation="MLRKLILTAISALSVFFISAQVTTSGISGIIKNSKNETLAGASV
                     QAIHVPSGTVYNTVALSD"
     gene            548..1753
                     /locus_tag="VGM24_05245"
     CDS             548..1753
                     /locus_tag="VGM24_05245"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY4154804.1"
                     /translation="MLDSIESAIEDIRQGRLVIVVDDEDRENEGDFVTAARNASPEIV
                     NFMSKHGRGLICVPLIEERCDQLSLELMVNNNTALHETAFTVSVDLLGHGCTTGISAH
                     DRALTIRALIDPATKPADLGRPGHIFPLRAKKGGVLRRTGHTEATIDLARLAGFEPAG
                     ILVEIMNEDGSMARLPQLKEIAKKFDFKLISIRDLIEYRLKTDSLIEEIVRVDMPTKY
                     GHFKLIAFSEKNTTNEHLALIKGEWKENEPVMVRVHSSCFTGDILGSLRCDCGDQLHK
                     AMQMVEANKSGIILYMNQEGRGIGLVNKLKAYKLQEDGMDTVEANIHLGFPMDKRDYG
                     IGAQILRYLGATRLKLISNNPRKRAGLSGYGLEIVETIPIQIPSNPHNERYLQTKRDK
                     LGHELFEEQ"
     gene            complement(1794..>5167)
                     /locus_tag="VGM24_05250"
     CDS             complement(1794..>5167)
                     /locus_tag="VGM24_05250"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY4154805.1"
                     /translation="QGTIKNFTTSGTIETNIGSMVTNVSMRFPTHAPSVYSGTVLTND
                     FELGEFMGDSSLGRVSFRGKVQGSGLTINTVSASLDGNVRQFNFRGYTYGNIFVNGQL
                     TKKRFNGTIISRDSNLLATLNGLIDLSGTTPRFDFDAEVGNADLLALHFLKDSVTFNG
                     KLRFNFSGNDIDHFLGKARIYDATVIKRKERLSFDSLVVESNIIDSSKTITVMSNEFE
                     GAIVGVFSIKELPAAFQEFLHRYYPSYIKPNTRKLTPEKFSFVITTRNVDPYLDLFDK
                     NLKGFNFTNVTGRISSEENLLNLNANIPQFSYKNISFYKVDLKGSGNFDSLSLDTRVA
                     EVYVNDSLHFPSTHIHLRSFGDLTDFKITTSANQTLNAANVSAQVQTLADGVKIRFNP
                     STLDINSKTWTIDKNGELLFSRNTVGANALKIYSGDQQLLISTHPSAEGNWNDVHMDM
                     HKVNIGDFAPFFVPDERLEGLLSGSVDITDPFNHTYLQFSGNAEDFRFSNDSIGSLTL
                     AADYSRQTGMVNAQVHSDNMDYHFDLKGIINTSDSAAQPINIMIPDLVNTKIDLLQKY
                     LGAVFSNVTGYATGQLQIVGTGHDLNYIGDLQLKDGSVHVNYTQCTYKIPSATVHLRA
                     DQIDFGSFQVLDTLGNKAEVTTGKLYHHSFRNMRYDFALNTSKLLLLNTNISDNNQFY
                     GSMIGGANIRLTGPQEDLQMYIKGEPTDSSNIFIPTSTSRESADADFIVWKVYGKEMK
                     PQKAAGAEDNFTVTVDVTANNYANIYLIIDPLTKDIIKANGHGNLQIQVGTNTNMNMR
                     GKYEIDRGDYNFSFQSFIRKPFVFKDGANNYIQWTGDPYNADINIQAVYEAENVQFGD
                     LGLNNTLLQGSEKLNTYHGPVWVVANLSDKLMHPAITFDIQLPPNSELRNNFVASVLL
                     TQLIGNDPNELNKQVAFLLVFNSFGPLTTGGAVFNANEAVGGIFFTSISGFISNALSS
                     QFSNIFQRVFRDKSIRVNFNTSFYNGTALQESVDRTYDRTNMNLSVIKSFLNERLTFT
                     VGSALDFGLTAQQAQYAAVQFLPNVTAEWKITENGHVVLTFFYRDSYNYLSVGNHTAN
                     SSGTSISYRRDFDRLDEFFKKKKKTDSTAVESGKQ"
BASE COUNT         1284 a         1202 c         1333 g         1348 t
ORIGIN      
        1 catccgaaag ggccaccgta ttgtacacgg ttccggacgg aacgtggatg gcctgaaccg
       61 atgcgccggc aagcgtttcg tttttcgaat ttttgatgat tccggaaatg ccggaagtgg
      121 ttacctgcgc ggagataaaa aatacggaaa gagcagaaat cgcagtaagg attagttttc
      181 ttaacataca agttagttgt ttacgctcgg caaaggaaca atattgaagg atcactaggc
      241 gcagcctgcc tattaaaaaa ttattaacag atggcaggca accgggaagg ggaatatatt
      301 tactaaagaa aagaaaaaaa aggaattcaa aatacgggga atggattttt tattttgaca
      361 aattgtaaac aaacatgaaa aaaaatagat tttctgtttt cggtcccctt taaccgccgg
      421 ttaattcaga aatggatccg gaagactgcc cggcctttcc ttccttcggt attccgctta
      481 atgcctgatc tgccgggctt agtaaaaaac cgggtaattg attaactttg tgtataactt
      541 ttttggcatg ttagacagta ttgaaagcgc tatagaggat atccggcagg gcaggctggt
      601 gatcgtggtg gatgatgagg accgggaaaa tgagggggat tttgttacgg ctgcccggaa
      661 tgcctcgccc gaaattgtca atttcatgag caagcacggg cggggtttga tctgtgttcc
      721 attgattgag gaacgttgtg atcagctttc cctggagctg atggtaaata ataatacagc
      781 ccttcacgaa acggcattca ccgtttccgt agatctgctg gggcacggct gtacgaccgg
      841 aatttcggca cacgaccgcg ccctgaccat cagggcattg attgatccgg caaccaaacc
      901 tgccgacctt ggccggcccg ggcatatttt tccgcttcgc gccaaaaaag gcggcgtgct
      961 caggcggacc ggtcatacgg aggccacgat cgacctggcc agactggccg ggtttgagcc
     1021 cgccggcatc ctggtggaga tcatgaatga agatggaagc atggcgcgcc ttccccaact
     1081 gaaggagatc gcaaagaaat ttgattttaa actgatttcg atccgggatt tgatagaata
     1141 ccggttaaaa acggactcgc tgattgaaga aattgtgcgc gtggacatgc ccaccaaata
     1201 cggtcatttt aagctcattg cctttagtga aaaaaatacg accaatgaac acctggcgct
     1261 gatcaaggga gaatggaaag aaaacgagcc tgtgatggtt cgcgtgcatt cctcctgttt
     1321 taccggagac atcctcggat cgctgcgttg cgattgcggg gaccagttgc ataaagccat
     1381 gcagatggtg gaagccaaca agagcgggat catcctttat atgaatcagg aaggccgtgg
     1441 cataggcctg gttaacaaat taaaagcata caaactccag gaagatggaa tggatacggt
     1501 ggaagcgaat atccatcttg gcttccccat ggacaaaaga gactacggca taggagccca
     1561 gatactccgg tacctcggcg cgacccggct gaaactgatc agcaataatc cgagaaaacg
     1621 ggccggcctg tcgggatacg gactcgaaat tgtggaaacc attccgatcc agattccgtc
     1681 caacccgcac aatgaacggt atcttcaaac caaacgcgat aagctggggc atgagttgtt
     1741 tgaggaacag tgagcggata cgggccatac cgcaggataa tttattcccc tttttattgc
     1801 ttcccgcttt caacggctgt ggaatccgtt ttcttctttt tcttaaagaa ttcatccagc
     1861 cggtcaaaat cgcggcggta ggaaatgctg gttccggaac tgttggcggt atggttgccg
     1921 acggataaat aattatagga atcgcggtag aagaaggtga gcaccacgtg gccgttttcg
     1981 gtgatcttcc attctgccgt cacattaggc aggaactgaa ctgcggcgta ttgggcctgc
     2041 tgcgcagtga gaccgaaatc cagggcactt cccactgtaa aggtcaaccg ctcgttgaga
     2101 aagcttttta ttaccgaaag gttcatgttg gtccggtcgt aggttctgtc cacactttcc
     2161 tgcagggccg ttccgttgta aaaagaagta ttgaaattca cccggatgct tttatcccgg
     2221 aatacccgtt gaaagatatt ggagaactgg ctgctgagcg cattggaaat aaaaccggat
     2281 atgctggtga aaaagatacc gcccaccgct tcattcgcgt taaacacggc ccctccggtc
     2341 gtaagcgggc cgaagctgtt gaacaccagc aaaaaggcaa cctgtttatt caattcgttc
     2401 ggatcgtttc cgatcaactg ggtaagcagg acggaagcga caaaattatt gcgcagctca
     2461 ctgttgggcg gcagctgaat atcaaacgtg attgccggat gcatgagctt gtcgctgaga
     2521 ttggccacca cccagaccgg tccgtgatag gtattcagtt tttccgaacc ctgcagtaaa
     2581 gtattattca gccccaggtc gccaaactgc acgttctcgg cttcataaac ggcctggata
     2641 ttgatatcgg cattgtacgg atcaccggtc cattggatgt aattattggc tccgtctttg
     2701 aatacaaagg gcttgcggat gaaggattga aaagagaaat tataatcgcc gcggtcgatc
     2761 tcatattttc cccgcatatt catattcgta ttggttccga cctggatctg caggttcccg
     2821 tgtccgttgg cttttatgat atccttggtg agcggatcaa tgatcagata aatattggca
     2881 tagttgtttg ccgtgacgtc cacggttacg gtgaaattat cttcggctcc ggctgctttc
     2941 tgtggtttca tttccttgcc gtagaccttc cacacgatga aatcggcatc ggcgctttcc
     3001 ctgctggtgc tggtggggat aaatatattg gaactgtcgg tcggttcccc cttgatatac
     3061 atctggaggt cttcctgggg accggtaagc cgtatgtttg ccccgccgat cattgaaccg
     3121 taaaactgat tgttatcgga aatattcgtg tttagcagaa gcagtttgct tgtattcaac
     3181 gcaaagtcat aacgcatgtt ccggaaagaa tgatgatata gtttcccggt ggtgacctcc
     3241 gctttgtttc ccagggtatc cagcacctgg aatgacccga aatcaatctg gtcggcgcga
     3301 aggtgcacgg tagcggaggg tattttatac gtgcactggg tatagttcac gtgcacgctt
     3361 ccatccttca actgcaggtc tcctatgtaa ttcaggtcgt gcccggttcc gacgatctgc
     3421 aactgtcccg tggcatagcc cgtcacatta ctaaaaacgg cgccaaggta tttttggagc
     3481 aggtcgatct ttgtgttcac caggtccggg atcataatat tgatcggctg agctgcgctg
     3541 tcgctggtat tgataattcc cttcaggtca aaatgataat ccatgttgtc ggaatggacc
     3601 tgggcgttga ccattcccgt ttgcctgctg taatccgctg ccagggtcag actgccgatc
     3661 gaatcattgg agaaccggaa atcttccgca ttgcccgaga actgcaggta ggtatgattg
     3721 aacggatccg taatatcaac cgatccgctg agaagacctt ccagccgttc gtccggcaca
     3781 aagaacgggg caaagtcccc gatatttacc ttgtgcatat ccatatgcac atcattccag
     3841 tttccttccg ccgacggatg cgtgctgatg agcagttgct gatcgccgct gtaaattttc
     3901 agcgcattgg cgccaacggt atttctgctg aacaggagtt ccccgttttt atcgatcgtc
     3961 caggttttgc tgttgatgtc cagtgtggac gggttgaacc ggatctttac gccgtcggca
     4021 agcgtttgca cctgggcaga cacgtttgcc gcgttcaggg tctggttggc gctggtggtt
     4081 atcttgaaat ccgtcagatc accgaaggaa cgcaggtgaa tatgcgtgga agggaaatgc
     4141 aggctgtcgt ttacatatac ctcggccacc cgggtatcca gggaaaggct gtcgaagttc
     4201 ccgcttcctt tcaggtccac cttataaaaa gaaatatttt tatagctgaa ctggggaata
     4261 ttcgcattca ggttcagcag gttttcctcg ctgctgatcc gtccggtgac gttggtaaaa
     4321 ttaaatcctt tcagattctt atcgaacaga tccaggtacg gatccacgtt ccgggtcgtg
     4381 atgacaaaac tgaatttttc cggagtgagt ttacgggtat tgggtttgat atagctggga
     4441 tagtaccggt gaaggaattc ctgaaacgca gccggtagct ccttgatcga aaatacgcca
     4501 acgatggcgc cttcgaactc attgctcatt acggtaatgg tcttgctgct gtcgataata
     4561 ttcgattcca ccaccaggga gtcaaaagaa agtctttctt ttctttttat aaccgttgcg
     4621 tcataaatac gggctttccc cagaaaatga tcgatgtcgt tacccgaaaa gttaaaacgc
     4681 agtttgccgt tgaaggtgac actgtctttc agaaagtgaa gggcgagcag gtctgcgttg
     4741 cccacttccg catcaaaatc aaaccgggga gtggttccgc tgaggtcaat gagtccgttt
     4801 aatgtagcca gcaggttgga atccctggat atgatcgttc cgttgaatcg ttttttcgta
     4861 agctgtccgt tcacaaatat gttaccatac gtgtatcccc tgaaattaaa ttgccggaca
     4921 ttgccatcca ggctcgcact gacggtattt atcgtgaggc cgctgccctg cacctttccc
     4981 cggaatgaaa ccctgcccag gctgctgtcc cccatgaatt cccccagttc aaaatcattg
     5041 gtgagtacgg tacccgaata gaccgatggc gcatgggttg gaaatcgcat actcacattc
     5101 gttaccatcg agccgatatt ggtttcaatg gttccggacg tggtaaagtt tttgatcgtg
     5161 ccctgga
//
LOCUS       DASYCU010000263         8131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1365843, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000263 DASYCU010000000
VERSION     DASYCU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365843"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGM24_05255"
     CDS             <1..261
                     /locus_tag="VGM24_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154806.1"
                     /translation="ASAGFKISWNDPLLPGTLFVFGCFFCFQWLLGFYEERLTNTLIP
                     VMVCIWALLLGQKIAEKKVIYVVGGLALTWQFCLLFSSGPYY"
     gene            321..1961
                     /locus_tag="VGM24_05260"
     CDS             321..1961
                     /locus_tag="VGM24_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154807.1"
                     /translation="MREKHPLFSPRVYAGLLLVLLIVTGIYCFIKPLVLSIDSVYGFL
                     AYKGSLFFHSFNVVQEVTPGDIGRLVPVFESWWSPGQWLFPGLLNYVFGIRLGLAGIL
                     VTIASLVSGFIGYYRVFDFYRFPRTIIFLSLLLIFSSSTLYYCFVVYQGGEILEFGIF
                     PWFLLYVIRIRRVSWWNLAGTGILFLLCFLGKTTLLLYCSFVLLAKIFQLNEGPAGTG
                     IRFSLKSLRLLLPALLCLVLVSVFFLSRGPRPVLIHHFGISADAVLVPLSSPLCSILS
                     VQQWIKRGEAVLSGLSPAGGYATLISHAVYLLVFAILVWLARYFFRNKKIDLSYKRLL
                     LILYTGLLVFFCFAYSFDANIDLSSRHFKLMGYLFIPGLITVLQEKAGSFRIQLATVF
                     FVLVSILDIFYLKEKWTKDRYVGINYFYRNCSPPGVKDKLDRESYQKIVEIDRGEPDG
                     KGEPVVFFVESTADIAIDLRHPVILQTPGDNLRDKVYRKNGAKLFVCISKSTLLQNAG
                     ILQWIFPDYRNFEPIGETGEYRYFLCSGRQEPAGTNNP"
     gene            2076..3188
                     /locus_tag="VGM24_05265"
     CDS             2076..3188
                     /locus_tag="VGM24_05265"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4154808.1"
                     /translation="MKNSLLSEDKSKNFSIGNYYPALDGLRGLAILLIILYHNFNYLP
                     FSRPTWISLDLFFVLSGFLITGILLKKRDSVRFLRNFYARRVLRIFPIYYLGIVLFLF
                     VLPRLISYPFSLNYFIAHQLWFWLEIQNWLFILRPDGNNNFLNHFWSLALEEQFYLLS
                     PWILLLIKPLQKVISFLLLLLLLLLFLRLILWFYQFKNFSYIDLYAFTRIDGLCVGSL
                     LAILTHQGNMVISRFNKGLLTLFFVLIFAVLPFCKIFYHTRLPYLACCLYPGIALFWG
                     WTVWRSIDPGTIINKIFTSRALIFFGKISYGLYIFHWPVNRLIRLSPGSASFEQSGLS
                     GNLLLSFLSTSIAILAAVISYYTYERYFLRLKKYFT"
     gene            3532..4920
                     /locus_tag="VGM24_05270"
     CDS             3532..4920
                     /locus_tag="VGM24_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154809.1"
                     /translation="MILTLNAGPTMMDDTDSYHIQMVKWIQEFGSVPGIANLHLRFGF
                     NSSWFVSIGLLTWPLHGLNTYLSLNGILSLWFCYFMLEKIFKFLRKDSLHKSVNPALG
                     CLVILVLGLVNWAMIRGCATSCNYDFISTCCIVILFFDLTDPSFKPDLEWLIWPVYLF
                     TVRMMNFPLLLFSLFYFWPAIRRISIRRFLACVFAAFFLVVPFIARNLILSGYAFFPV
                     YQLDWFSFDWKADKSSVAAISEYIKYFNRVNPAFRSLAETSQLSFPRWIGNWFTYLFP
                     FDKLLIVLSFFGYGFVLVWRRRILSNSQRKFMVVMIIQLICWFIMAPDPRFAQGAMLF
                     GIYMAIQEAPALPVNLKNFFKYSTGLLAGLVLFYSGSKMVRDKNYRNLLSPHPLPVPA
                     VQTLVLGHIQLHIPSKVLNNWNPRCYDVALPCLYKLDPRVEARGKGIGEGFRIRPGSR
                     NIQQEGEYKIKE"
     gene            4928..6595
                     /locus_tag="VGM24_05275"
     CDS             4928..6595
                     /locus_tag="VGM24_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154810.1"
                     /translation="MVFELISWIYISIICLIWGNGFVRLIFRGGVNISELDLPVLCFV
                     GLSVIGVVSLYLSLFIPLNGWVKGILQVPVLLYFLSSSNRITFFNQVKNAFAKLAPTD
                     RFLLIAGIVMLLFLSSSPVIHPDSLNYHIYSIQIFNKYGMIRGIANLQPELGFQSLWF
                     GVMSVFDLSFFQPAVSYPLSACVLCWFIIFLVSGSVTRGARAEAGIPAFWYLALLIFA
                     FVSWTQVRLTASSASPDFIVSLCLLLSFYFFTRKALPGEVEFPLFFALFFAVIAISIK
                     LSAIFIILLPAYVLVYGAVTKKFFLVRSGLLLITLFLAPVIARNIISAGYPLYPSAFA
                     DVFHGDWKPDESKLILFRHYITDYARYPVSRSVAEGAYAASFQDWLPVWWRHLYIIDK
                     IMMLLIVCGGLADLILLRKWLKNFTARRAAAFVISLTGAVCWFINAPDPRFGTGFLLP
                     LIYFQYAPFLISGKQWFYKITGIVKFSFTLLIVTYIVYRGIYFFHPGQLIFPEGIKES
                     RPASADCRYKIKRMLFRDSAGQAGIADSCRTFIFRGSSLKEGFKPAE"
     gene            complement(6626..>8131)
                     /locus_tag="VGM24_05280"
     CDS             complement(6626..>8131)
                     /locus_tag="VGM24_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154811.1"
                     /translation="PHAIAFLFKKNDPSFIPKQTITTNIFDKTFISTTCPSPATSGTI
                     TSPLFGPAKKWNELHWRGTSTESPSTDSVGLQVYGVDTSGNSTLIYTLHQANPDYDIS
                     SVSPSQFPYLQLKLTATDTVHATPFQLNYWRLTYTPVPEGALAPNIFLTGRDTVELGE
                     QLEFGIAFKNISLQKFDSLKISLEIIDKNNVTHIIPYPKTKPLISGDTVKILYEIDSK
                     DYAGLNTIYLDVNPANDQPEQYHFNNFLFKNFYVHEDKTNPLLDVTFDNVHILNEDIV
                     SAKPHIMIKLKDESKFLLLNDTSVLKVQLRYPDQSLHPYYFNTDTLRFTPAAGSSDNT
                     ASVEFNPVFTNQIDPQGDEYTLIVTGSDKSGNPSGTIQYQVTFKIITKAMISNMLNYP
                     NPFTTSTAFVFTVTGSDVPQNIKIQILTITGKIVREITKDELGPLHVGRNITEFKWDG
                     TDMYHQRLANGVYLYHVVTNLNGKSLDKYQAQGDNTDQFFNKGYGKMYLMK"
BASE COUNT         1736 a         1811 c         1962 g         2622 t
ORIGIN      
        1 gcttctgccg gattcaaaat atcatggaat gacccgcttt tgccgggaac gctgttcgta
       61 ttcggctgct tcttttgttt tcaatggctg ctcgggtttt atgaggaaag gcttacgaat
      121 accttgatcc cggtcatggt atgtatctgg gcgttgctgc tggggcaaaa gatcgcagag
      181 aaaaaggtga tttatgtggt gggcggatta gccctgacct ggcaattctg tctgctgttc
      241 agttccgggc cttattattg atcaatgctt ttctccggca aaatccggct gcatgaccat
      301 gctttaatta tgactgaaat atgagggaga aacatccgct tttttcaccc cgggtttatg
      361 ccggcctgct attggtcctg ctgatagtga ccggcattta ttgtttcata aaaccacttg
      421 tcctgagcat cgacagcgtc tatggatttc tcgcatacaa aggcagtttg ttttttcatt
      481 cgttcaatgt ggttcaggag gtaacgccgg gcgatatcgg caggctggtt ccggtctttg
      541 aaagctggtg gtccccgggt caatggctgt ttcccggtct gctgaactat gtctttggca
      601 tccggctggg cttggccgga atcctggtta ccatcgcatc cctggtcagc ggcttcatcg
      661 ggtattaccg ggtttttgat ttttaccggt ttccgcgaac cattatcttt ctctccctat
      721 tgttaatttt ttcaagttcc accttatact attgctttgt tgtttatcag ggtggtgaaa
      781 tcctggaatt cggaatattt ccctggttcc ttttatacgt aatccggatc cggcgcgtgt
      841 cgtggtggaa tctggccggc accgggatcc ttttcctgtt gtgtttcctt gggaaaacca
      901 ctttactgct ttattgcagc ttcgttttac tggctaaaat atttcaactg aacgaggggc
      961 ctgccggaac cggtatccgt ttttccctga agagccttcg gttgttattg cctgcattgc
     1021 tttgcctggt cctggtctct gtattttttc tttcccgggg gccgcgtccc gtcctgattc
     1081 atcattttgg aatatcggcc gatgcggtcc tggtacccct gtcttctcct ttgtgcagca
     1141 tcctctccgt tcagcaatgg ataaaaagag gcgaggcggt tttatccggt ctgagccctg
     1201 ccggcggata tgccacgctg atttcccatg ccgtgtatct tctcgttttt gcgatcctgg
     1261 tctggctcgc gcgctatttc ttccggaata aaaagattga cctttcgtat aaaaggcttc
     1321 tcctcatttt gtataccggt ttactggtct ttttctgctt tgcttattcg ttcgatgcaa
     1381 atattgatct cagcagccgt catttcaaac tgatgggtta tttatttatt cccggtctga
     1441 tcaccgtatt gcaggagaaa gccggatcat tccggattca gctggcaacg gtcttttttg
     1501 tcctggtatc catcctggat attttttatc taaaggaaaa atggacaaaa gaccggtatg
     1561 tcggcatcaa ttatttttac cgcaattgca gccctccggg tgtaaaggat aaactggata
     1621 gggagtctta tcaaaaaatc gttgagattg accggggtga accggatggg aagggagaac
     1681 cggtagtatt ttttgtcgaa tctaccgcag acattgccat cgatctccgc catcccgtta
     1741 tcctccagac gccgggggat aaccttcgcg ataaagtcta ccggaaaaac ggcgccaaac
     1801 tgttcgtgtg tatttcgaaa agcaccctgc tacagaatgc cgggatcctg caatggatat
     1861 ttccggatta ccggaatttc gaacccattg gcgaaacggg ggaataccgc tattttttgt
     1921 gtagcgggcg gcaggagcca gcggggacaa ataatccata gactgccctg ttgcgccggg
     1981 ataaaatggt cttaaaatga gtgtatcttg aggaatgata gaacaggggc atcctcccat
     2041 cggaaatcag aagctataaa ctatccggca attaaatgaa aaattcactt ttaagtgagg
     2101 ataaaagcaa aaatttttct attgggaatt attacccggc gctggatggt ctccgcggtt
     2161 tagccattct gctgattatt ctctaccata attttaacta cctgccattt tccaggccaa
     2221 cctggatcag cctcgatctt ttttttgtgt tatcgggttt tctgatcacc gggatattgc
     2281 tgaaaaaaag agattcagtg cgatttctcc ggaatttcta tgcccgacgg gtcctccgga
     2341 tatttcccat atattattta ggtattgttc tgtttttatt tgtgctgccg cgcctgattt
     2401 cctatccgtt cagtctgaat tattttatag ctcaccagct atggttttgg ttggaaattc
     2461 aaaactggtt attcatcctg aggcccgacg ggaataataa ttttctgaat catttttggt
     2521 cgcttgccct ggaagagcaa ttctatctgc tcagtccctg gattctttta ttgattaaac
     2581 cacttcaaaa agtgatcagc tttctgttgc tattgctcct gctcctgctg tttttgcgac
     2641 tgatcctgtg gttttatcaa tttaagaatt tctcctacat agatctttat gcatttacga
     2701 gaatagatgg tttgtgcgtg ggaagcctgc tcgccattct tacccatcag ggaaatatgg
     2761 tgatcagccg gtttaataag gggttgctga ccttattttt tgttttaatt ttcgccgtgc
     2821 tgcccttctg taagattttt tatcataccc gcctgcccta cctggcctgc tgtctctatc
     2881 cgggtattgc cctgttttgg ggatggaccg tatggcgttc gattgatccg ggaacgatca
     2941 taaataaaat cttcaccagc cgggcgctca ttttctttgg aaagatctct tatggtttat
     3001 acatttttca ttggcccgtg aaccggctca tcaggttaag cccgggatct gcttcctttg
     3061 agcaatccgg actttccggt aatctgctcc tgtctttcct ttcaacctcc attgctatcc
     3121 ttgctgcggt tatcagttat tatacctatg aacgatattt tctgagacta aaaaagtact
     3181 ttacttaaac gaagcctccc catgctttat ttaatactct gttattttct gctcacctgt
     3241 gcttgtttat gggccggcct cttgatgtac agtttcattc ccggtagggg agcccaaaac
     3301 cggtctgtga tacacttcct gataacggga ctgatcggtc tgacagcggt tgggcaatgg
     3361 attgttttat ttttcccgct cactttattg tcccttgtac tgatcttatc cgggtgcgtt
     3421 gtaattacgc ttttcagaaa aaaatttatt ctggatgagg ggaaaaggtt ttggctaggc
     3481 ttaaggaacc tggatcggat ggtctgggtt tgcggattct gttttttgat catgatcctc
     3541 accctgaatg ccggcccgac catgatggat gacacggaca gctaccacat tcagatggtg
     3601 aaatggattc aggaatttgg atcggtcccg ggtattgcca atctccacct gaggttcgga
     3661 tttaactcat cctggttcgt ttcgatcgga ctattaacct ggcctttgca tggcctgaat
     3721 acctatctct ccctgaacgg gattctttcc ctctggtttt gctatttcat gctggaaaag
     3781 atttttaaat ttctccggaa ggattctttg cacaaatccg taaacccggc attgggttgc
     3841 ctggtcatcc ttgtcctggg ccttgtgaac tgggccatga tcaggggatg cgccacgagt
     3901 tgtaattatg attttatttc gacctgctgc atagtcattt tattcttcga tttaacggat
     3961 ccttctttta aaccggatct ggagtggttg atctggccgg tctatctgtt tacggtcagg
     4021 atgatgaatt ttcctctgct tcttttcagt cttttttatt tttggccggc catccggagg
     4081 atttccataa gaagattcct cgcttgtgtt tttgccgcct tttttttagt ggttcccttt
     4141 atagccagaa atcttattct ttcaggatat gcctttttcc cggtctacca gctggactgg
     4201 ttttcctttg attggaaagc agataaatct tccgtggccg ccatttccga gtatatcaaa
     4261 tacttcaacc gggtcaaccc ggctttccgg tccctggcag aaaccagtca attgtctttt
     4321 ccgcgctgga tcggaaattg gtttacctat cttttcccat ttgataagct gctaatcgtg
     4381 ctttcttttt ttggatatgg atttgttttg gtgtggcgga ggagaatttt gtcaaacagc
     4441 cagcggaaat ttatggttgt aatgatcatt cagctgatct gctggtttat tatggcaccg
     4501 gatccccggt ttgcgcaggg ggctatgctt tttggaatat acatggccat tcaggaggca
     4561 cctgctttac cggtgaacct gaaaaacttt tttaaatatt ccactggtct cctggctggc
     4621 ctggtcctgt tctactccgg atcaaaaatg gtccgggata agaattaccg gaatttgctc
     4681 agtccgcacc cactgcctgt gcccgctgtt caaacactgg tgctcggcca cattcaattg
     4741 catattcctt ccaaggtatt aaacaactgg aatccgcgtt gttatgacgt ggcattaccc
     4801 tgcctgtata aactggatcc gcgggtggaa gccaggggaa aagggatcgg ggaaggtttc
     4861 cggatcaggc cgggaagccg gaatattcag caggaaggcg agtataaaat aaaggagtaa
     4921 acggcttatg gttttcgaat taatttcctg gatatatatt tctatcatct gcctgatctg
     4981 gggaaacggt tttgtgcggc tgatcttccg gggaggggtg aatatttccg aacttgatct
     5041 tccggtcctt tgtttcgtgg gtctgtccgt gatcggagtg gtttctcttt atctttccct
     5101 ttttattcct ttgaacggat gggtgaaggg aatcctgcag gttcccgttc tcctgtattt
     5161 tctgagcagt tctaaccgga ttactttttt taaccaggta aagaacgctt ttgcaaaatt
     5221 ggctccgaca gaccggtttc ttttgattgc cggcatagtg atgctgcttt tcctttccag
     5281 ctctccggtc attcatccgg attctttgaa ctatcatatc tattccattc agatttttaa
     5341 taagtacggg atgatccggg gaatcgccaa tcttcagccg gaattgggtt ttcagagtct
     5401 ttggtttggg gtaatgagtg tttttgactt gtcttttttc cagccggctg tttcctatcc
     5461 actaagcgcc tgcgttttgt gctggtttat tattttcctg gtctccggat ctgtaacgcg
     5521 cggtgcccgg gcagaggctg gaattccggc tttctggtat ttggctttgc tgatctttgc
     5581 cttcgtttcc tggacgcagg tcaggctgac ggcatcttct gcaagcccgg acttcatcgt
     5641 tagcctttgc ctcttacttt ctttctactt ttttacccgg aaagcccttc cgggggaagt
     5701 ggaattccct ttatttttcg cgttgttttt cgccgttatc gccatttcca tcaaattgtc
     5761 cgctattttc attatcctgc ttccggctta tgttcttgtt tatggggcgg taacaaaaaa
     5821 gttttttctt gtccgaagcg ggcttttgct gataaccctt ttcctggccc cggttatcgc
     5881 ccggaatatt atttcagccg gctatccttt gtatccgtcg gcttttgctg atgtatttca
     5941 cggggactgg aaaccggacg agtccaaact aatattgttc cggcattata ttacggatta
     6001 tgcccggtac ccggtttcaa gatcagttgc ggaaggggcc tatgcggctt cctttcagga
     6061 ctggttgccg gtatggtgga ggcatcttta tattattgat aaaataatga tgctgctgat
     6121 tgtttgcgga ggattggcgg atttaattct tctgagaaaa tggctaaaga attttacggc
     6181 gcgaagagcg gcagcttttg tcatttcgct taccggtgcg gtatgctggt tcataaatgc
     6241 cccggatccg agatttggaa ccggatttct ccttccgctg atttatttcc agtatgcgcc
     6301 ttttctgatt tccggcaagc aatggttcta taaaataacc ggcatcgtaa aattttcttt
     6361 cacgcttctg attgtgactt atatcgtata ccggggcatt tacttctttc accccggcca
     6421 gctgattttc ccggaaggga taaaagaatc ccggcccgct tcggcagatt gccgttataa
     6481 aataaaaaga atgttgttcc gggattctgc tggtcaggca ggaatcgcgg acagctgccg
     6541 gacttttatt tttcgcgggt cttcccttaa agaggggttt aaaccggcgg aataatgacc
     6601 tgcctgttga acggggaata ccccgtcatt tcatcaagta cattttacca taccccttgt
     6661 taaagaactg atccgtatta tctccctgtg cctggtattt atccagcgac ttgccattca
     6721 gattcgttac cacatggtag aggtatacgc cgttggccag ccgttgatga tacatatccg
     6781 ttccgtccca tttgaattcg gttatgttgc gcccgacatg caggggtccg agttcgtctt
     6841 tggtgatctc ccggacaatt ttaccggtaa tggtcaggat ctggatcttt atattctgcg
     6901 gcacatcgct tccggtgacg gtgaatacga atgcggtgga tgtggtgaat gggttcggat
     6961 aattgagcat attcgaaatc atggccttgg tgatgatctt gaaggtcacc tggtattgaa
     7021 ttgttcccga aggattgccg ctcttatcgg agccggtcac aatcagggtg tattcatccc
     7081 cctgcggatc gatctgattg gtgaaaaccg ggttgaattc cacagaagcg gtattgtcgg
     7141 agctgcccgc agccggcgtg aaccggaggg tatcggtatt gaaataatac ggatgcaggg
     7201 attgatcggg ataacgcagc tggaccttaa gcaccgaagt gtcattcagc aaaagaaact
     7261 tggactcgtc tttcagcttg atcataatat gcggcttggc tgaaacgatg tcctcattca
     7321 ggatatgcac attgtcgaag gtcacatcca gcagcgggtt tgtcttatcc tcgtggacat
     7381 agaaattctt aaaaagaaaa ttattaaaat ggtattgttc cggctggtcg ttagccgggt
     7441 tcacatccag gtaaatggta ttcagcccgg cgtaatcctt gctgtctatc tcgtacagga
     7501 tcttaaccgt atcgccactg ataagcggtt tcgttttcgg gtatggaatg atatgggtca
     7561 cattattctt atcaatgatt tccagggaga ttttcaggct gtcgaatttc tgcagactga
     7621 tattcttaaa ggcgataccg aattccagtt gttcaccgag ttccaccgta tctcttcccg
     7681 tgagaaaaat attgggtgcc agggctccct cgggaaccgg cgtataggtt agtcgccaat
     7741 agttcagctg aaagggcgtg gcatgaaccg tgtctgtggc cgtcagtttt aactgcaaat
     7801 aagggaattg agatgggctg acagaagaga tatcgtaatc cgggttagcc tgatgaagcg
     7861 tgtaaataag ggtggaattt ccactggtat ccacgccata aacctgcagg ccgactgaat
     7921 ccgttgaagg ggattcggtg gaggttcccc gccaatgcag ttcgttccat ttttttgcag
     7981 gaccgaaaag aggagaggtg atcgtgccgg aagtggcggg agaaggacag gttgtggaaa
     8041 tgaacgtctt gtcgaaaata ttcgtcgtga tggtttgttt cggaataaag gaagggtcat
     8101 ttttcttaaa cagaaaagcg atggcgtgcg g
//
LOCUS       DASYCU010000264         4991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4263131, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000264 DASYCU010000000
VERSION     DASYCU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4263131"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGM24_05285"
     CDS             complement(<1..347)
                     /locus_tag="VGM24_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4154812.1"
                     /translation="MPDHEFTGKVAIITGAGQGIGFEMARQLVASGAHIVLNDLDARL
                     AGEAVQRIRQEREAGCLAMGGDSGDLSFIQKMVAAAVSEFGKLDIVIANAGITLFGDF
                     LHYPPESFFRVLQV"
     gene            complement(367..2070)
                     /locus_tag="VGM24_05290"
     CDS             complement(367..2070)
                     /locus_tag="VGM24_05290"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEY4154813.1"
                     /translation="MKLRSSNWFGRTGKDGFIYRSWMKNQGIPAHEFRGKPVIGICNT
                     WSELTPCNAHFRELAESVKHGIYEAGGFPVEFPVMSLGETLIKPTAMLFRNLVSMDVE
                     ESIRANPMDGVVLLCGCDKTTPALIMGACSVNLPSIVVSGGAMLTGKFRGKDIGTSDV
                     WRFSEAQRAGKMSQEDFEFAEACMSRSRGHCAVMGTASTMASMTEALGLSLSENAAIP
                     AADSRRKALAHLSGMRIVEMVREDLKLSDVLTRQAFENAIITNAAIGGSTNFIIHLMA
                     IAGRIGVKLGMDDFDTHTRGIPLIANLQPSGKYFMEDLYYAGGIPAVMKELDSFLNRD
                     ALTVSGKPIADQYKNSACYNREVISGIADPFNRVAGIRVLKGNLCEDGAVIKPSAASA
                     ALLQHTGKAVVFENIEDFKKRIDDPALEVDENSILVLKNVGPKGYPGMPEVGNMSLPK
                     KLLEKGVTDMVRISDGRMSGTGFGTVILHVSPEAFVGGTLAIVKNGDRISLDVFKNSL
                     QVELSEEEIARRKKELPAAGITGRGYSWLYQQHVEQANRGADLDFLKGGSGSEVLKDS
                     H"
     gene            2247..2651
                     /locus_tag="VGM24_05295"
     CDS             2247..2651
                     /locus_tag="VGM24_05295"
                     /inference="COORDINATES: protein motif:HMM:NF026420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HEY4154814.1"
                     /translation="MKIFNYFFVFVFFVFAALQYNDPDPYIWMPIYLYAAALCWLSTK
                     GKFSPPAYWAGFAVYGIYALYKMFDENGLIDWIRKHHAQNIADTMKAETPWVEETREF
                     FGLVIVIGVLLINYIYLKKHRNSGPPLRSDKL"
     gene            2992..3879
                     /locus_tag="VGM24_05300"
     CDS             2992..3879
                     /locus_tag="VGM24_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEY4154815.1"
                     /translation="MKKICAGVFSTAVATAVLVTACKKDNSGSSHNNTPPGEQTVSVY
                     LNDDPAPDLSSVLIDVRTVEVKVDTGKTDHDDAYYDDDHEEDSAGADHHGDQFGAWDT
                     LSINPGVYDLLKLKNGTDTLIAKGLAKKGKISRLRITLGSDNKVWTDSSHSYPLTICN
                     TSPYVYVRVKSDAVEAVTNGENRIRIDFDAARSIEGKDGQFCLRPRLKAYADPNTGKL
                     EGNVQPGEAHAKILVFNNTDTSFAFPEENGSFEIRGLLPDTYTVLYKATAPYMDSTVS
                     NIQLNAGAETKLPDLSLHK"
     gene            complement(3927..>4991)
                     /locus_tag="VGM24_05305"
     CDS             complement(3927..>4991)
                     /locus_tag="VGM24_05305"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY4154816.1"
                     /translation="KTFPLIPPAHKKISFSLAAVCAFMEVGKKLVFSEPALDDSLKRI
                     LAWYKAGKFGKGLYEASLEYGKEVADTLLRWANKDHYKETRRLPRYRFLKEEGKWIPT
                     PPAYMDAIEPYWNRIRLIALDSCTQFRPPPPFDFSKDTASTFYRQAYDVYETGNHLTD
                     EQRAIANFWDCNPFAVNMQGHLHFAEKKISPGGHWISIVGVVTRSRNKSVLEASSVYT
                     LTSIALFDAFISCWDEKYRSNVIRPETYINDYIDESWRPVLQTPPFPEYVSGHSIISA
                     AAAEVLTAIMGPSVGFDDDTEKEFGQPVRHFDSFIQASREAAVSRLYGGIHYRAAIDN
                     GLDEGREIGKWVLNKIQLKK"
BASE COUNT         1238 a         1347 c         1172 g         1234 t
ORIGIN      
        1 acctgcaata cccggaaaaa agattccgga ggatagtgga gaaagtcccc gaacagggtt
       61 atgccggcat tcgcgataac aatatccagc tttccgaatt cggaaacggc cgctgcaacc
      121 atcttctgaa taaaagacag atcgccggaa tcgccgccca tggcaagaca accggcttcc
      181 ctttcctggc gaatgcgttg aaccgcctcc ccggccagcc gggcgtccag atcgtttaaa
      241 acaatatgag ctccggatgc caccagctgc cgggccatct cgaatccgat gccctgcccc
      301 gccccggtaa tgatggccac ctttccggta aattcatgat ctggcatacc catccggttt
      361 taatttttag tgagaatctt tcagcacttc actgccgctt cctcctttta aaaagtcaag
      421 gtctgcgccc cggttcgcct gttcaacgtg ctgctggtaa agccagctgt acccgcggcc
      481 ggtgatgccg gcagccggca attctttttt ccgccgcgct atttcctctt cggaaagttc
      541 gacctgcagg gaatttttga aaacatccag gctgatccgg tcgccgtttt ttacaatggc
      601 gagggtgccc cccacaaaag cctccgggct tacatgcagg atcacggtgc cgaatccggt
      661 tccgctcatt ctgccgtctg aaatccggac catatcggtc actccttttt ccagcagctt
      721 tttaggcagg gacatattac ccacttccgg catgccggga tatcctttgg ggcccacatt
      781 tttcagcacc aggatactgt tttcgtccac ctcgagggcc ggatcatcga tccgcttttt
      841 gaaatcctca atattttcaa acaccaccgc tttcccggta tgctgcagta aagcggcact
      901 ggctgcagac ggtttgatca ccgccccgtc ctcgcataga ttacccttca atacccggat
      961 gcccgccacg cggttaaaag gatccgcaat gccggagatc acctcgcggt tatagcaggc
     1021 gctattttta tattgatctg ctatcggttt accgctgacg gtaagcgcgt ccctgtttaa
     1081 aaaggaatcc agttctttca tcacggcagg tatcccgcct gcataataca gatcttccat
     1141 aaaatatttt ccggaaggtt gcagattggc gatcagtgga attccccggg tatgcgtatc
     1201 gaaatcgtcc atgccgagtt taaccccgat ccgtcccgca atggccatca ggtgaatgat
     1261 aaaattggtt gagccgccga tcgcggcatt cgtaataatt gcattttcaa atgcctggcg
     1321 ggtgagcaca tccgataatt tcagatcctc ccgcaccatc tccacgatgc gcatgccgga
     1381 aagatgcgcc agcgcctttc gccgggaatc tgcagcggga atagccgcgt tttcggaaag
     1441 ggaaagcccc aaagcctccg tcatggatgc catggtggaa gccgtgccca taaccgcgca
     1501 atgcccccgg gagcgcgaca tgcaggcttc cgcaaattca aaatcttcct gcgacatttt
     1561 cccggcccgc tgcgcctccg agaaccgcca cacatcggag gtgccgatat cctttcccct
     1621 gaatttcccg gtgagcatgg ccccgcccga aaccacaatg gacggaagat tcacgctgca
     1681 ggcgcccatg atcaaagcgg gtgttgtttt atcgcagcca cagagcaata ccacaccgtc
     1741 catcggattg gcgcggatgg attcttccac atccatgctg accaggttgc ggaagagcat
     1801 ggccgtcggt ttgatcaggg tttcacccag cgacatcacc ggaaattcca ccgggaaacc
     1861 tccggcttca taaatcccgt gttttacgga ttccgccagc tccctgaaat gtgcgttaca
     1921 gggagtcagc tccgaccagg tattgcaaat gccgatcacc ggtttcccac gaaattcgtg
     1981 agccggtatg ccctggttct tcatccagga gcgataaata aacccatcct tgcccgtgcg
     2041 tccgaaccag ttactgcttc ttagtttcaa ttcttcttat tttaataaat ttcgggaaaa
     2101 caagatcctc ttgatggcac aatttagcaa accttttaat ggccgctgac cggatttttc
     2161 caattaaatt tgtaccggaa gaaaaatcag ggaacagccc ctgcgtcaaa ttattaataa
     2221 ttagccgtcg ccagcctgaa ccgctcatga aaatattcaa ctattttttt gtattcgttt
     2281 tttttgtatt tgctgcgctg caatacaatg atccggatcc gtatatctgg atgccgatct
     2341 atctgtacgc agccgccttg tgctggcttt ccacaaaagg caaattttct ccgccggcct
     2401 attgggccgg atttgccgtg tatggcatct atgcccttta caagatgttt gatgaaaacg
     2461 gattgatcga ctggatccgg aaacatcatg cccagaatat cgcggatacc atgaaggcgg
     2521 aaacgccgtg ggtggaagaa acccgcgagt tttttggtct ggtcatcgtg atcggcgtgt
     2581 tgctgatcaa ttatatctat cttaagaaac accggaattc aggtcctccg ctccggtctg
     2641 acaagctcta aggtcccggg cttattctta acatatcgtt taatgatcac cggctaccgg
     2701 tgattatacc atgcagttta attttcttca aacggattaa cttctttcag gccagcgatt
     2761 taaagcaatt gttttttggg aaagaaaatt ccgggatccg ggtcaggcat ttccggattt
     2821 caaacaccgg aaaatactgg tatagtattt ggctacggaa acctgcccct acaccccttt
     2881 cccaaagagg tcaccttaat acattacaaa agcgattcat ttgtaaaaat ttactccttt
     2941 cgattctctt ctcttatttt ttaattttta aagaaaacga ctatgaaaca aatgaagaaa
     3001 atttgtgcgg gagtattttc aactgctgtc gccacggcgg ttctcgtcac cgcctgcaag
     3061 aaagacaatt caggttcttc ccataataac accccgccgg gggagcagac cgtttcggtt
     3121 tacctgaacg atgatccggc tcctgatctg tccagtgtac tgatcgacgt gcgcacggtg
     3181 gaagtgaaag tggataccgg gaaaacggat catgatgatg cctattacga cgacgaccat
     3241 gaagaagaca gcgccggtgc cgatcatcat ggcgatcagt ttggagcctg ggatacgctg
     3301 agcattaacc ccggagtgta cgatctatta aaattgaaga atggaaccga taccctgatc
     3361 gcaaaagggc tcgctaaaaa aggtaagatc agccggctcc ggataaccct gggctctgat
     3421 aataaagtat ggaccgacag ctcgcattcc tatccgctta ccatctgcaa taccagtccc
     3481 tatgtatatg tgcgggttaa gtccgacgcc gtcgaagccg tgacgaatgg ggaaaaccgg
     3541 atccgcattg attttgatgc cgcaagatcc attgaaggga aggacggaca attctgcctc
     3601 cggcccaggc tgaaagcgta tgccgatccg aataccggga aactggaggg gaatgttcag
     3661 cccggcgaag cccatgcaaa aatactggtc ttcaacaata cggataccag ctttgctttt
     3721 cctgaagaaa acggcagttt tgaaatccga ggtctgctgc cggacacgta tactgtttta
     3781 tacaaagcga cggctccgta catggacagc accgtcagta atatccagtt gaatgccggc
     3841 gcggaaacaa aattgccgga tctcagcctg cataagtaaa atccaccaag ataaattcag
     3901 ccaaggccgg acagcgatcc ggccttttat tttttcagtt gtattttatt caacacccat
     3961 ttcccgattt ccctgccctc atccaatccg ttgtcaatgg cagcgcgata atgaattccg
     4021 ccgtaaagcc gtgaaacagc cgcctcccgg gaagcctgga taaaagaatc aaaatggcgg
     4081 acgggctgtc cgaattcctt ttccgtatca tcatcaaaac caacggaagg gcccattatc
     4141 gcagtgagca cttcggccgc agcagccgat atgatactat gcccgctaac atattccgga
     4201 aagggtggcg tttgcaatac cggccgccag ctttcatcga tataatcatt gatataggtt
     4261 tccgggcgaa ttacattact gcggtatttt tcatcccagc aactgatgaa cgcatcgaac
     4321 agcgcgatgg aagtgagcgt atacaccgaa gacgcttcca gaacagactt gttccggctc
     4381 cgggttacga cgccaacgat ggatatccaa tgccctcccg gggatatttt cttttctgca
     4441 aagtgcagat gaccctgcat gttcacggca aacggattgc agtcccagaa attggcaatg
     4501 gctctctgtt catccgtcag gtgattaccc gtttcataaa catcataagc ctgccggtag
     4561 aatgtgctgg ccgtatcctt gctgaaatca aagggcggag gaggccggaa ctgagtgcag
     4621 gaatccagcg cgatcaaccg gatccggttc cagtaaggct ctatggcatc catataggcg
     4681 ggcggagtcg ggatccattt accttcttct tttaagaagc ggtaccgcgg tagccggcgc
     4741 gtttctttat aatgatcctt attcgcccaa cggagaagcg tatccgcaac ttccttcccg
     4801 tattccaggg aggcctcgta aagccccttg ccgaatttcc ccgctttata ccaggcaaga
     4861 atgcgcttta acgaatcgtc cagcgccggt tcggagaaaa ccagtttttt ccccacctcc
     4921 atgaatgcac atacggcagc cagcgaaaaa gatatttttt tatgcgccgg aggaataagc
     4981 ggaaatgttt t
//
LOCUS       DASYCU010000265         6037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4698833, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000265 DASYCU010000000
VERSION     DASYCU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4698833"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="VGM24_05310"
     CDS             <1..1831
                     /locus_tag="VGM24_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4154817.1"
                     /translation="WFQSSADLTYKIPFVQGLSVKGLYSFDFTLNDNKMYPKAYNLYN
                     YNSATDTYTPSLNQSPSTVRREFYEYPTNLEQLSLNYNHSFGEHNITALLLYEQSVRK
                     ADNFYAQRELSIPVDQLLAGNSLNQQGSMNTGQLYTYANDAYVGTLTYDFRSKYLGKF
                     GFRYDGSSRFGNGRQWGFFPEGEIGWRVSEENFWKNSRALSFINSLKLRGSYGITGDD
                     GASTYQFVSGYTYPASGSSTGLPPGAVFDGTFINGVQNKGIPNPNISWYTSKTLDAGI
                     DVVAWNGLLGFTFDYFVRNRSGLLATELLTLPDVVGANLPQQNLNGDRTKGFDFEINH
                     KNHIGKLGYSVTATFAMTRTMNTVKVQAKAGNSYLNWLNNTGDRYNNIYFGYGSDGQY
                     ESYQSIENSGIYTGKNVVVGDYRYEDWNGDGQIGVDDSHPIATTGLPLITYGVNIGGT
                     YEGFDFSFLLQGASLVNASYLEQLSTPLWAGGNALSQFLDRWHPADPSADPYSPSTVW
                     VPGNFAYTGTVPYTNTLANLHNAAYLRLKSAEIGYNLPGKWVKRSGLGNVRVFVNAYN
                     LLTFTGLKYLDPEHPSAISAVDQQYGYAYPLDKIYSFGVNVKF"
     gene            1842..3758
                     /locus_tag="VGM24_05315"
     CDS             1842..3758
                     /locus_tag="VGM24_05315"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154818.1"
                     /translation="MKIKIHLLFLVLVAALPACRKNLDVPPENIVQDKDIFNSTSGIT
                     AYMARIYSEIPMEDFKWGPQSGFKTFFYGSPYAITGEAISRDQQNSTETFGYWADAYS
                     LIRECNYFMETLPTYAANFTTAQVNGWIGEARFIRAFTYFALVKRYGGVPIVDKVLTA
                     PGESIDEITGDIDSYNIPRSSEEAVYDFVGSDLDSAFAGLPETNQGGRANKYAAAALK
                     SRAMLFAGTIAKYNTISLTDGSTEVCGIPAARANDYFKAAYDAAALLDGKYSLYKKSW
                     SATDKAAQAQNFADLFLDASSPENIFIREYHYPDAAHWYDANQVPRQLWNGGYAAETN
                     PTLDFVEMFEGLPKNPDSTFRTLDADGHYILYDNLGDPFVNAEPRLKGTIIFPNDEFK
                     GQTIEIRRGIYTGPSAGGINKLTPEGSTAAYPGDNLVSSATDAQTAYTLPDGTKMNPA
                     GLSGIFTGNVAGCISGFTIRKYLDPNLATADVATNHSDQSWIEIRYAEVLLNQAEAAY
                     ELYDGGVGGNYLTTALTDLNLIRERAGASQATFGDLTSVDIIRRERRKELAFENKTWW
                     DLRRWRISDKEQNGTIYRILNPIFSSTANKYFFDDRFDERNSRYTFDPRWYYEQIPAS
                     AIAKSSALVQNPGY"
     gene            3770..4540
                     /locus_tag="VGM24_05320"
     CDS             3770..4540
                     /locus_tag="VGM24_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3823 domain-containing protein"
                     /protein_id="HEY4154819.1"
                     /translation="MKRIRYNMLIILALLMAGSCKKKLDNYPGPTETLKGSIMDMGTG
                     KNVQSEVSGDNGKGTRIKLLETSWSSNPTPLYLAGKQDGTYENTKVFAATYKMTAEGA
                     FVPLVQTNTAGEVTVDQSQTVDVKGGTTTVNFNVEPFLEVEWVGEPVINSDGSITAQA
                     KLTRGTTDADFQQDVTDLWLYVSPTEYVGDNNYDARYAQHITYSGTDGDAILGTTVTL
                     TTKGGALPKKDYYLRLGARINYGLKQYNYSEPKVVTIP"
     gene            4575..>6037
                     /locus_tag="VGM24_05325"
     CDS             4575..>6037
                     /locus_tag="VGM24_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154820.1"
                     /translation="MKCFNVCATAVAGKWVLGFFSIGIIITEILCSQALAIPPAPSFP
                     SSFDCKVQPDYKYRKDGKPGREIRIIFRDSKLYGPATIKVECNSIVEETSVDHPEGIG
                     ELSVLLPEGAGVDMECQVRISLTADKKELYRSIIVEPQRQWTVYIYPHSHVDIGYTNT
                     QDFVRRLHMRDIDVAIDLAKKTQHYPEGSRFIWNPEASWVTENYLRAASPEKKAAFID
                     AVKKGWICLDGSYCNTNTSACSDEELLRLFHTSDQLQKLTGVPIKTMVQFDVAGAAWG
                     ITQAAFQMGIQAFFYYPNLGTVRKPWENKPFYWVSPDGKSRILFYQAFPYGFGYTIKG
                     AKIGIGVVQGRKDSMLDRITTKDPTADFLDPLIFNETAKLEAEKSPYDIFVMPWSLAD
                     NSLIDADLPDAVRLWNEKYAFPKLIISGSQRILHDFESRYGASLPEIKGDYTEYWTDG
                     LGSDARRVGLDRHATEDLVQMETAWSMLDYNRPSPRK"
BASE COUNT         1586 a         1519 c         1556 g         1376 t
ORIGIN      
        1 atggttccag tcttcggcag atctgacata taagattccg ttcgtgcagg gcttaagcgt
       61 aaaaggtctc tacagtttcg actttaccct gaatgacaac aaaatgtatc cgaaggccta
      121 caacctgtat aactataaca gcgctaccga tacgtatact ccaagtctga atcagtcgcc
      181 aagcaccgtc agaagggaat tctatgaata cccgaccaac ctggaacaac tctccttaaa
      241 ctataaccac agtttcgggg aacataatat aaccgcatta ttgctttatg aacaaagcgt
      301 gcgcaaagcc gacaactttt atgcgcagcg ggagctttcc ataccggtcg atcagctgct
      361 tgccggaaac agtctcaatc agcagggcag catgaacacc ggccagctct acacctatgc
      421 aaatgatgcc tatgtgggaa cgttaaccta tgatttcaga tcaaaatacc tgggcaaatt
      481 cggtttccgc tatgatggct cttcccggtt cggcaacggc aggcaatggg gattctttcc
      541 ggaaggagaa atcggatggc gggtttcaga agaaaatttt tggaagaatt cccgcgcgct
      601 ttcattcatc aatagtttga aactgcgcgg ctcctatggg ataacaggtg atgacggggc
      661 atcgacctac cagtttgttt ccggctacac ttatccggcc agcggatcct ccaccggttt
      721 gcctccgggc gctgtctttg acggcacctt catcaatggg gtgcagaata aaggtattcc
      781 caatcccaat atcagctggt acacgtcaaa aacactggat gccggtatcg acgtggttgc
      841 ctggaacggt ttgctgggat tcacttttga ttattttgtc cgtaaccggt ccgggctgct
      901 ggcgacagag ctcctgacgc tgcccgatgt ggtgggagcg aacctgcccc agcagaatct
      961 caatggcgac cggaccaaag gttttgattt tgagatcaat cataaaaatc atattggcaa
     1021 gctcgggtac tcggtcaccg ccacattcgc catgacccgc acgatgaata cggtcaaggt
     1081 tcaggccaag gccggaaatt cctacctcaa ctggttgaac aataccggcg accggtacaa
     1141 caatatctat ttcggctacg ggtcagacgg gcaatatgaa tcctatcagt caatagagaa
     1201 cagcggtata tataccggta aaaatgttgt tgttggcgat taccggtacg aggactggaa
     1261 tggtgacggg cagatcggcg tggacgatag ccacccgatt gccacaaccg gcctgccgct
     1321 cattacctat ggagtcaata tcggcggaac atacgaagga ttcgatttca gcttcctctt
     1381 gcagggcgcc agcctcgtga atgcatccta tcttgaacaa ctgagcacac cgctttgggc
     1441 agggggtaat gccctgtcgc agttcctcga ccgctggcat ccggccgatc cttcagccga
     1501 tccctatagt ccttctacgg tttgggttcc cggaaatttt gcctacaccg gaacggttcc
     1561 ttataccaac accctcgcca atctgcacaa tgcggcttac ctgaggctta agagcgccga
     1621 aataggctac aaccttcccg gcaaatgggt aaagaggtca ggtcttggga atgtgcgcgt
     1681 tttcgtcaat gcctataacc tgttaacctt taccggcctg aaatatcttg atccggagca
     1741 tccttccgcc atctccgccg tcgatcagca atacggttac gcctatccgt tagataagat
     1801 atattctttt ggagtaaacg tgaaattcta aataaaaagt catgaaaata aaaattcatt
     1861 tgttgttcct tgttctggtg gctgccttgc cggcctgcag gaaaaacctg gatgtgcctc
     1921 cggagaacat cgtgcaggat aaggacatct tcaacagcac cagcggcatc accgcttata
     1981 tggcccgcat atacagtgaa atacccatgg aagattttaa gtggggccct caatcggggt
     2041 ttaagacctt tttttacggt tcgccctatg ccattaccgg ggaggcgatc agccgcgacc
     2101 agcaaaacag cacggaaaca ttcggctact gggcggatgc ctattccctg atccgcgagt
     2161 gcaattattt tatggaaacc ctgcccacct acgccgccaa ctttacgaca gcccaggtga
     2221 atggctggat cggcgaagcg aggttcatcc gtgcctttac ctatttcgcc ctggtcaagc
     2281 gctacggtgg tgtgccgatt gtggacaagg tgcttaccgc gcccggagaa agcattgatg
     2341 aaattaccgg cgatattgat tcgtacaata taccccgttc ctccgaggaa gccgtctatg
     2401 attttgtggg gtccgacctt gattctgctt tcgccggact gcccgaaacc aatcagggcg
     2461 gccgggccaa caaatacgcc gcggcagcgc ttaaatcaag ggccatgctg tttgcgggaa
     2521 cgatcgcaaa atataatacg atcagcctga ccgacggttc tacggaggtt tgcggcattc
     2581 ccgcggcccg ggccaacgat tattttaaag ccgcctacga tgcggctgca ttactggatg
     2641 gcaaatacag cctctacaaa aaaagctggt ccgccaccga caaagcggcg caggcccaga
     2701 actttgcgga cctgttcctg gatgcttcca gtcccgagaa catttttata agagaatatc
     2761 actatcccga tgcggcgcac tggtatgatg ccaaccaggt tccacgccag ctgtggaacg
     2821 gaggatatgc cgccgagacc aatccgaccc tggattttgt ggagatgttc gaagggcttc
     2881 ccaaaaatcc ggatagcacg ttcaggaccc tggatgccga cggacattat attttatatg
     2941 ataacctggg cgatcctttt gtcaatgccg agccaaggct caaaggcacg attatctttc
     3001 ctaatgatga attcaagggg cagacgattg aaatacgtcg cggcatatat accggccctt
     3061 ccgccggcgg catcaataaa cttactccgg aaggttcaac cgcggcttat ccgggagata
     3121 acctggtgag ttcggcgacc gacgcacaga cggcctatac cttgccggac ggcaccaaaa
     3181 tgaacccggc gggattgagc ggaatattca ccggcaatgt tgccggctgt atttccggtt
     3241 tcaccatccg gaaatacctg gatcccaatt tagctaccgc cgatgtggcc acgaatcact
     3301 cggaccaaag ctggatagag attcgctatg cggaagttct cctgaaccag gccgaagccg
     3361 cgtatgaact ctacgatggc ggagtcggcg gcaattacct gaccacggcc ttgaccgatt
     3421 taaacctgat ccgcgaacgg gcgggtgctt cgcaggctac cttcggtgat ctgacaagtg
     3481 tggatattat ccgccgggaa agaagaaagg aacttgcttt tgaaaataag acctggtggg
     3541 atctgcgcag atggcggatc agcgacaagg agcagaatgg tacgatttat cgcatactga
     3601 atccgatctt ttcatccacc gcaaacaagt atttttttga cgaccggttt gatgaaagaa
     3661 acagccgcta cacgttcgat cccagatggt attacgaaca gataccggca tcggccattg
     3721 ccaagagttc agccctggtt cagaatcccg gatattaaac tatataaaaa tgaaaaggat
     3781 acgatataac atgctcatta ttctggccct gctcatggcc ggatcatgca aaaaaaaact
     3841 ggataattac cccggcccca ccgagacgct caaggggagc atcatggata tgggcacagg
     3901 gaaaaacgtg caaagcgaag tttcggggga caacggaaag ggaacccgga tcaaattatt
     3961 ggagacgagc tggagcagca atcccactcc gctttacctg gccggcaaac aggacggcac
     4021 gtatgaaaat accaaggtgt ttgccgcaac ctataaaatg accgccgagg gtgcatttgt
     4081 accgctggta caaacgaata ccgccggcga agtcaccgtc gatcaaagcc agacggtcga
     4141 cgtgaaagga ggaaccacca cggttaactt caacgtggaa cccttcctgg aagtggaatg
     4201 ggtgggcgag ccggtcataa attctgacgg cagcatcacg gcccaggcaa aactgacccg
     4261 gggaacaacc gatgcggact ttcagcagga cgttacggat ctctggctgt atgtgagccc
     4321 cacggaatat gtgggagaca ataactatga cgcgcggtac gcccaacaca taacctacag
     4381 cggtacggac ggtgacgcca tcctgggcac tacggtcacg ttaaccacta aagggggcgc
     4441 gctgcccaaa aaagattact acctgagact gggggcaagg attaactatg gcctgaaaca
     4501 atataactat agcgaaccga aagtggtaac gatcccctga aatatggtta gggacaataa
     4561 ctaaaagaca aggaatgaaa tgtttcaatg tatgcgcaac ggccgttgca ggaaagtggg
     4621 tattgggttt tttttcgata gggatcatca tcactgaaat tttgtgcagt caggccctgg
     4681 ccataccgcc ggcgccatct ttcccctctt cctttgactg taaagtgcaa ccggattata
     4741 aataccggaa ggatggaaag cccggacggg agatcaggat cattttcagg gattcaaaac
     4801 tctatggacc ggcaacaata aaagtggaat gcaattccat tgtagaagaa acttccgttg
     4861 atcatcccga agggatcggt gagctgtctg tattgcttcc ggagggtgcc ggcgtggata
     4921 tggaatgcca ggtgcggatt tccctgacgg ctgataaaaa agaattatac cgttcgatca
     4981 tcgtggagcc ccagcggcaa tggacggtct atatttaccc gcattcgcat gttgatatcg
     5041 gttataccaa tacgcaggat tttgtaagaa ggctgcacat gcgggatatt gatgtggcca
     5101 tcgatctggc gaaaaagacg cagcattatc cggaaggttc gcgatttatc tggaacccgg
     5161 aggccagctg ggtaacggaa aattacctgc gtgccgcctc tcccgaaaaa aaggcggcat
     5221 tcatcgatgc ggtcaaaaag ggctggatct gcctggatgg cagctactgt aatacaaata
     5281 ccagtgcctg ttccgatgag gagctgctcc gcttatttca tacttccgac cagttgcaaa
     5341 aactcacggg cgttccgata aaaacaatgg tgcagtttga tgtagccggc gctgcctggg
     5401 gaattacaca ggctgctttc cagatgggga tccaggcctt cttttattat cctaacctgg
     5461 gtacggtaag aaagccctgg gagaacaaac ccttttattg ggtatctccc gacgggaagt
     5521 cgaggatttt attttaccag gcatttcctt acggatttgg atacacgata aagggtgcga
     5581 aaattggtat cggcgtcgtg cagggaagaa aggacagcat gcttgaccgg atcacgacaa
     5641 aggacccgac cgccgatttt ttagatccgt tgatctttaa tgaaacggct aaactggaag
     5701 cagagaagtc cccctacgat atttttgtca tgccctggtc gctggcagat aattccctga
     5761 tcgatgcgga tctgccggat gccgtgcgtt tatggaatga gaaatacgcg ttccccaaac
     5821 tgatcatatc cggttcgcag cggattctgc atgattttga aagccgatac ggagccagtc
     5881 tccccgagat aaagggagat tataccgaat actggacgga tggcctgggt tcggatgcaa
     5941 gaagggttgg cttggaccgc catgccacgg aggacctggt gcaaatggag acggcctggt
     6001 ccatgctgga ttataatagg ccctcccccc gaaaaca
//
LOCUS       DASYCU010000266         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4409743, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000266 DASYCU010000000
VERSION     DASYCU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4409743"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VGM24_05330"
     CDS             complement(<1..211)
                     /locus_tag="VGM24_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154821.1"
                     /translation="MQPVSRLTALAFLCCFLVLGCRSRERSGFPRILIFTTAGIHPDS
                     LSAGVEAIRKLGSENHFETDTTSDPS"
     gene            445..2922
                     /locus_tag="VGM24_05335"
     CDS             445..2922
                     /locus_tag="VGM24_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEY4154822.1"
                     /translation="MFTAVARVKMVLFSVLSCICFSGFAQQDDLKLWYRTPAGDDWEK
                     ALPIGNGKLGAMVYGNPARECFQLNECTIWTGGPNRNDNPDALRSLPEIRQLIFQGKE
                     KEAQALAANTLQTKKSNGQMYQPLGNLYLDFPGHENVADYYRELDISKSVATSSYRVG
                     NIKFTRTVFASAPANAIIIRLDADKPGRLSFTASLISPQKNAHITVNGGKISIHGSTE
                     GHEGVEGGRLKFNGIVRIVPQGGTLSAGDSGISVRKANHVLIYIAIATNYVSYRDLSA
                     DEIKRSENDLSKAVAKNYRELLTDHIKNYQKYFSRVSLDLGTSGAVKNPTDIRLREFA
                     RGRDPQFAALYFQYGRYLLIASSQPGGQPANLQGIWNDKMDPPWDSKYTININTEMNY
                     WPAEKDNLPEMAEPLIGMVRDLSETGRETARTMYGARGWMAHHNTDLWRITGPVDGIY
                     WAVWSMGGAWLCQHVWEKYLYSGDMEYLRSVYPLLKGAATFFIDDLVEEPTHHWLVIC
                     PGTSPENAPSVRPDVSFAAGTTMDNQIVFDMLSVALRAAQVLGTDAAFSDTLRKIRSR
                     LPPMHIGQYGQLQEWLEDLDNPGDHHRHISQLYGLFPSNQISAFRTPELFRAAHNTLI
                     QRGDISTGWSMAWKVNWWAHMLDGNHAYKLITDQLSPAGAHKEGGGTYDNLFDAHPPF
                     QIDGNFGCTSGITEMLMQSGDGAIFLLPALPDAWKTGSVRGLRARGGFEIRELEWKNG
                     KIAKLVIRSGLGGNCRLRVPNALKNAGTLPLSKAEGVNPNPFYYTNQTADPVIADRAK
                     LADLRLPETMLYDLKTKPGETYTLTAN"
BASE COUNT          761 a          790 c          813 g          687 t
ORIGIN      
        1 gcgacggatc ggaggtcgta tctgtttcaa agtgattttc actgcccagc ttccggatgg
       61 cttccacgcc cgcggaaaga gaatccggat gaatacccgc cgtggtgaag atcagtatcc
      121 ggggaaaacc ggaccgctcc ctgctccggc agccgaggac taaaaaacag caaaggaagg
      181 ctaaggcagt cagtctggat acaggttgca tttcttatat tttataaaaa aagcaaataa
      241 ccatgtaacc gttaaccagc cggttaataa acgtattaat ttataaatta agcgataatt
      301 agtaccctga ttaatggatt aaatgttaat tgcacgggta ttatccacac cggcctgcag
      361 aacggatggg agacctcccg ccagagaata atttttttcc tttgttccgg gcgggataac
      421 ggggaaataa tttaaattca gctgatgttt accgcggttg ccagggtgaa aatggttttg
      481 ttttcggttc tttcctgtat ctgcttcagc ggatttgctc agcaggatga cctgaaattg
      541 tggtaccgga cgccggccgg cgacgactgg gaaaaagcct tacccatcgg caatggaaaa
      601 ctcggcgcaa tggtttatgg gaatcctgcg cgggaatgtt ttcagctgaa tgaatgtacg
      661 atctggaccg gcggacctaa ccgcaacgat aatcccgatg cgctgcgctc ccttcccgaa
      721 atccgccagc tgatctttca gggaaaagaa aaggaagcgc aggcgcttgc cgccaacacc
      781 ctacagacga aaaaatcaaa cgggcagatg taccagcccc tcggtaatct gtacctggac
      841 tttccaggac atgaaaatgt tgcggattat taccgggaac ttgacatatc aaaaagcgtg
      901 gccaccagca gctatcgggt aggtaacata aaattcaccc gcaccgtttt tgcttccgcc
      961 ccggcaaatg ccattatcat ccggctggat gcggataagc ccggccggct ttcgttcact
     1021 gcatccctga tctctcccca aaaaaacgca catattactg tgaacggggg gaaaatcagt
     1081 attcacggca gtacggaagg gcacgaagga gtggagggag gaagactgaa attcaatggg
     1141 atcgtccgga tcgttccgca ggggggaact ttatccgccg gtgattccgg catttccgtg
     1201 cgcaaggcaa accatgtcct catttacata gccatagcga ccaattatgt cagctaccgg
     1261 gacctcagcg cggatgaaat taaacgttcg gaaaacgatc tgtcgaaagc agtggcgaaa
     1321 aattaccgcg aactgctgac ggatcatatt aaaaactatc aaaaatattt tagccgggtc
     1381 tccctggatc tcgggacttc cggcgccgtg aaaaacccga ccgatatccg cctgcgcgaa
     1441 tttgcccggg ggcgggatcc gcaattcgcc gctttgtatt ttcaatacgg aagatacctg
     1501 ctgatcgcct cttcgcaacc cgggggacaa ccggccaatc tgcagggcat ctggaacgat
     1561 aagatggatc cgccctggga cagcaagtac accatcaaca tcaacaccga gatgaattac
     1621 tggcccgccg aaaaggataa tctgcccgaa atggcggagc ccctgatcgg gatggtgcgg
     1681 gatctttctg agaccggaag ggaaacggcg cggaccatgt atggcgcaag aggctggatg
     1741 gctcaccaca ataccgatct ctggcggatc accgggccgg tggatggaat ttactgggct
     1801 gtgtggagta tgggcggcgc ctggctttgc cagcacgtgt gggagaaata cctgtacagc
     1861 ggggatatgg aataccttcg ttctgtatat ccgctgctga agggggcggc cacttttttc
     1921 atagatgacc tggtggaaga acccacgcat cactggctcg ttatctgtcc cggcacttct
     1981 cccgaaaacg cgccttctgt gcggccggat gtttccttcg ccgccggaac aacaatggat
     2041 aaccagattg ttttcgatat gctttccgtt gccttgcgcg cggcacaggt attaggaacc
     2101 gacgcagcat tttccgacac gctccggaaa atcaggagcc ggcttcctcc catgcacatc
     2161 ggacagtacg gacagttgca ggaatggctg gaggacctcg acaatcccgg agatcatcac
     2221 cgccatattt cccagcttta cggtctcttt ccttccaacc agatctctgc tttccggacg
     2281 ccggaattat tccgggctgc ccataacacg cttattcagc gcggcgatat ttccaccggc
     2341 tggagtatgg cctggaaagt gaactggtgg gcccatatgc tggatggcaa tcatgcctat
     2401 aaattaatta ccgaccagct ttcgcccgca ggtgcccata aagaaggcgg aggcacctat
     2461 gataatttgt ttgatgctca tccgccgttt cagatcgacg ggaattttgg ctgtacttcc
     2521 ggaattacgg aaatgctcat gcaaagcggt gacggggcga tatttttatt gccggcactt
     2581 ccagatgcct ggaagaccgg cagcgttcgc ggtttgcggg caagaggagg gtttgagatc
     2641 agggagctgg aatggaagaa cggaaagatc gctaaactgg tgatccgttc cgggcttgga
     2701 ggaaattgcc ggctgcgggt tccgaacgcg ttgaaaaatg ccggcaccct tccgctcagc
     2761 aaggcggaag gcgtcaaccc gaatccattc tattatacca atcaaacggc agatccggtg
     2821 atcgccgacc gggcaaagct ggctgacctg cgcctgccgg aaacgatgct ttatgacctg
     2881 aaaacaaaac cgggcgaaac ctacaccctg accgccaatt gaattttatg aaatattttt
     2941 ccggatggtt gatctctttc ttctttgcgg gctgggtgaa tggacaggct cccctgaccc
     3001 gcgtagcctg tatcggcgcc agcattacct ccggcgccac ccttcctaat c
//
LOCUS       DASYCU010000267         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2959019, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000267 DASYCU010000000
VERSION     DASYCU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959019"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VGM24_05340"
     CDS             complement(<1..1330)
                     /locus_tag="VGM24_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY4154823.1"
                     /translation="MRHALPVITIFDIGRTNKKRLLFNARYELLEEQSIPLPETTDED
                     GFPSEDLSLLTQWVKESFGEILLRKDIELKAVNVSAYGASLVHLNSALKPFTPLYNYL
                     KPYPDGLLQEFREKYDADGQLAKQTASPELGHLNSGMQLYWLKKNQPAVFRQIRYSLH
                     LPQYISFLLSGFCASDLTSVGCHTRLWDFEKQDYHDWVKKEELTGILPALCPGDFVSL
                     QRPDQIPVGVGLHDSSAALIPYLSAFKEPFVLISTGTWSITLNPFNHQPLTAPELEQD
                     CLCYLSFQGKPVKASRLFAGHDHEVLTKKLASCFHCPADHYLSVIPDERIIEKWTTAT
                     EQEIAGACSAPGGFNSYEEAYHVEMIRIMRMQQRSTRLVLEPGSIKKIFVDGGFGKNR
                     LFMKLMARSFPEMEVFAASLAHASAFGAALSIHHHWNREELPADLLALERY"
     gene            complement(1330..>1536)
                     /locus_tag="VGM24_05345"
     CDS             complement(1330..>1536)
                     /locus_tag="VGM24_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HEY4154824.1"
                     /translation="LESAREQNEVVTAQEILQGAFRTDVRPLVAEARRRAGGALDPLH
                     LFRSAGIRKKLVKERGEKSFATGL"
BASE COUNT          376 a          392 c          379 g          389 t
ORIGIN      
        1 aatatctttc cagcgccagc aggtcggccg gcaactcttc ccggttccag tgatgatgga
       61 tactcagtgc tgcaccgaag gcagaagcat gagcgaggga agcggcaaag acttccattt
      121 cgggaaaact ccgggccatc agcttcatga acagccggtt cttaccgaag ccgccatcca
      181 cgaagatctt ttttatgctt cccggttcca gcaccagccg ggttgatctt tgctgcattc
      241 gcatgatccg gatcatttca acatggtacg cttcttcata agaattgaaa ccgccgggag
      301 cggaacaggc accggctatt tcctgttccg tggccgttgt ccatttttca ataatccgtt
      361 cgtccggaat cacggataaa taatgatcag cggggcaatg gaaacaggaa gccagttttt
      421 tggtaaggac ctcatgatca tgccctgcaa aaagtctgga cgctttcacc ggctttccct
      481 gaaaggacaa ataacaaaga caatcctgtt ccagttccgg tgcggtcaaa ggttgatgat
      541 taaacgggtt cagggtgatt gaccaggtgc cggtggatat caaaacaaaa ggttctttaa
      601 atgcggataa ataaggtatc aatgccgccg agctgtcatg caatcctacg ccgaccggaa
      661 tttgatccgg tctttgcagg gacacgaaat ctcccgggca aagcgccggc agtataccgg
      721 tcagttcttc cttcttaacc caatcgtgat agtcctgctt ttcgaaatcc cagaggcggg
      781 tatgacagcc cacactggtc agatcggagg cacagaatcc cgacaataaa aagctgatgt
      841 attgcggcag atgaagggaa taccggatct gccggaaaac agccggctga ttttttttca
      901 gccagtacag ctgcatacct gaattcaaat ggcccaactc cggagacgcg gtctgtttgg
      961 ccagttgtcc gtccgcatca tatttctccc tgaactcctg cagtaatcca tccggatagg
     1021 gctttaaata attatagagc ggcgtgaatg gttttagcgc gctatttaaa tgaaccagcg
     1081 aagcgccata ggcagacacg ttcactgctt tcagctcgat gtctttccgc aataaaattt
     1141 caccgaaact ttcttttacc cactgcgtta gcagggaaag gtcttctgag ggaaatccgt
     1201 cttcgtccgt cgtttccgga agcggtatgc tttgctcttc gaggagttca tagcgggcat
     1261 tgaataaaag ccttttctta ttggtccggc caatatcaaa aatggttatg acaggaaggg
     1321 catgcctcat tataatccgg tagcaaatga tttttctccc ctttctttca ccagtttttt
     1381 tcgtataccc gccgaacgga aaagatgaag ggggtccagt gcgccgcccg ctctccggcg
     1441 tgcttcggcg accagggggc gaacatccgt gcggaaggcg ccctgtaata tttcctgggc
     1501 ggtcaccact tcattttgtt ccctggctga ttccag
//
LOCUS       DASYCU010000268         5146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5134730, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000268 DASYCU010000000
VERSION     DASYCU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5134730"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGM24_05350"
     CDS             complement(<1..398)
                     /locus_tag="VGM24_05350"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEY4154825.1"
                     /translation="MKKALKYSLRILAVLIGLLVLVSFGGWIYLKQHKAEIIQYIKTE
                     SAKNLNGQLSIGDIGFSLFHTFPKVSVSVDNVRLRDSLWTVHHHELLNARKAYASLDI
                     FELITGHLRISKIILEDASVYIYTDSSGYT"
     gene            complement(593..1066)
                     /locus_tag="VGM24_05355"
     CDS             complement(593..1066)
                     /locus_tag="VGM24_05355"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEY4154826.1"
                     /translation="MNIHKMGSNKRIALIAHDNKKSELIEWAIYNKDALSRHRLYATG
                     TTGKMLEKALNQTITKYMSGPLGGDQQIGARIAERKIDVLIFFWDPMEAQPHDPDIKA
                     LLRLGVVWNIPFACDRSTADFLLTSPLMHQDYEVIQPDYSVYLKRPQTGTPKVEN"
     gene            1202..1795
                     /locus_tag="VGM24_05360"
     CDS             1202..1795
                     /locus_tag="VGM24_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4154827.1"
                     /translation="MRKLWLTLVAALCCTGAICQTNNTPLFSRFTVSNAGNSSAILKW
                     TVSCVEPGEYFIVEKSADGIRFETMSAIASGTMASDTNFSVTDNAVGNGMIYYRIRIS
                     EGNGNLVYSKMVSTSATLLGDFRFYPNPVDKLLIVRSAHPVSIQVLDAYGIVWFSQAL
                     DAGLQIINVSTLQKGNYILKATDKETNNILSEQLIKN"
     gene            complement(2057..3061)
                     /gene="recA"
                     /locus_tag="VGM24_05365"
     CDS             complement(2057..3061)
                     /gene="recA"
                     /locus_tag="VGM24_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY4154828.1"
                     /translation="MSNPEKLKALKLTIDKIDKDFGKGSVMMMNEKSTEPMEVVSTGS
                     IGLDVALGIGGLPRGRVVEIYGPESSGKTTIATHVIAEAQKKGGMCAIIDAEHAFDSN
                     YARKLGVDVDSLLISQPDYGEQALEIADRLILSGALDVVVIDSVAALVPKGELEGEMG
                     DSKMGLHARLMSQALRKLTATISKTNTICIFINQLREKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRMAQIKDGEEAIGNHVKVKVVKNKVAPPFRSAEFDIIFGEGISKMGEVID
                     MGVEFGIVQKSGSWFSYGADKLGQGRDAVRQLLLDNPELATEIEGKIRAKIKEIQEAE
                     "
     gene            3319..4008
                     /locus_tag="VGM24_05370"
     CDS             3319..4008
                     /locus_tag="VGM24_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEY4154829.1"
                     /translation="MNRIDRLFGILTLLQSKKYVTGEKIAEKFSISVRSVYRDIKALN
                     EQGIPVSFEPYRGYFVVNGYFLQPVSFSTEEANALLLMESIVSGFADKSIKLNYSNAL
                     TKVKAVLRSSQKDSIEFLADNIKFQAPPCFIQHMDYLSDIQKAIARRLVLEIEYTNLQ
                     KEVSKRKAEPIGLVFYAFNWHLIGWCHLKKGYRDFRVSRILRLHCTQENFLNKKHMEM
                     NEYMKQLPVNF"
     gene            4081..4575
                     /locus_tag="VGM24_05375"
     CDS             4081..4575
                     /locus_tag="VGM24_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY4154830.1"
                     /translation="MNSIISYFQNQLQEEAVTARKMLERVPADKLDWQPHPKSMSLVR
                     LATHVAEIPNWIPMITESDELDFAKGDYSPHPINSNKDLLDYFEKNVKDGKAALEKAS
                     DEILDKPWTMRSGEDVFIRSTKGEVLRMSLSQIIHHRAQLGVYLRLLDVPIPGSYGPS
                     ADEQ"
     gene            complement(4630..>5146)
                     /locus_tag="VGM24_05380"
     CDS             complement(4630..>5146)
                     /locus_tag="VGM24_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154831.1"
                     /translation="KDPVSGRWNCAEITSLQSFEYGTYQFVVEGAIDKFDKNIVLGLF
                     NYSGNGGLDEMDIEIARWGNPAYPNLNYTIWPAKRGLKDSATVKEFSLKSNLSTHYFK
                     RNADTVICASFDGLSTDPKDEIFSSVFTKPASISHLAMPVHINFWLFGGNGPENQQPV
                     EVVIRSFQYKP"
BASE COUNT         1265 a         1316 c         1210 g         1355 t
ORIGIN      
        1 ttggtataac ctgaggaatc ggtgtagata taaacggagg catcctccag gatgatctta
       61 ctgatacgga gatggccagt gatcaactca aagatatcca gactggcata ggctttccgg
      121 gcattgagga gttcgtgatg atgtaccgtc cacaaactgt cccggagcct cacattatcc
      181 accgaaacgg agactttagg aaaagtatgg aacaggctga agccgatatc cccgatggat
      241 agttgcccgt tcaggttttt cgcggactct gttttaatat attgaatgat ctccgccttg
      301 tgttgtttca ggtaaatcca gcctccgaac gatactaaaa caagaagccc gataagaact
      361 gccaggatac gaagactgta ttttagggct tttttcattt tttgagaagc gcttacgcac
      421 aggggtgaat gatttctcca gacccgatga tcttctcacc ggtctgatcc gattgctgcc
      481 agagcaacct cattccggac acaccagcgg aatcaatttc ccgccttccg gcaaaaagag
      541 cggaaaacca agccggcagg cttcctgaac ggagctggca ggctttgcgc gttcagtttt
      601 caaccttggg cgttccggtc tgcggtctct tcagataaac cgaataatcg ggctggatga
      661 cttcatagtc ctggtgcatc aggggtgagg taagcaaaaa gtcggccgtg gaccggtcgc
      721 aggcaaaggg gatattccag acaacgccaa ggcgcagcag ggctttgata tccggatcat
      781 ggggctgggc ttccatagga tcccagaaaa aaatgagcac gtcaattttt ctttcggcta
      841 tccgggcgcc gatctgctga tcgcccccga gcggtccgct catgtacttg gtgatggtct
      901 ggttcagggc cttttccagc attttgccgg tggtgccggt ggcatagaga cgatgcctgg
      961 aaagcgcgtc cttattgtat atggcccatt caatgagttc agatttcttg ttatcgtgcg
     1021 cgattaaagc gatccgttta ttgctgccca tcttgtgaat attcattccg gattatttat
     1081 tgcaagatag gagattgcct gttgggccgg gagcaaacgg gctggtaaaa aaatggcttt
     1141 ccccgatagt tcatgtaacg gcatgaaatt tgttcacatg ttttccccat tttactgacc
     1201 catgaggaaa ctttggttaa ccctggtagc agccctgtgc tgcacaggag cgatttgcca
     1261 gacaaataac accccgctgt tctcccgttt tacggtaagc aatgcaggaa acagttcggc
     1321 cattctgaaa tggacggtca gctgcgttga accgggcgaa tattttatcg tggagaaaag
     1381 cgcggacggg atccgctttg aaaccatgag tgcgatcgct agcggcacca tggcctccga
     1441 taccaacttt tcggttaccg acaatgccgt cggaaacggc atgatttatt accgcatccg
     1501 gatttctgaa ggaaatggca atcttgttta ttcaaaaatg gtcagcacca gcgcgaccct
     1561 gctgggtgat tttcgttttt atcccaatcc ggtcgacaag cttttgattg ttcgcagtgc
     1621 ccatcccgtt agtatccagg tactggatgc ctatggtatt gtctggttca gccaggcgct
     1681 ggatgccgga ttgcagataa ttaatgtttc aacattgcaa aaaggcaatt atatactcaa
     1741 ggccacggat aaggaaacca ataacatcct ctcggagcaa ctgatcaaaa actaagtttt
     1801 ccccgttttc ttcccaaaaa aatcagatca atatctgata agatatttag ccttttctgt
     1861 ttgcagatct cactcttttc tattatcttt acctcggttt ttcataggat attggatttt
     1921 aaaacggggt tgaattttta ttctgacccc tttttttatg ccttgttcca gccggtccgg
     1981 atgctgagtt accaaaaaaa acaaaggcca tcttttcaga cagcctccgt acttaccaac
     2041 ccaatcaaat cggcggttat tccgcctcct gaatttcttt aattttcgcc cggatctttc
     2101 cttcaatttc agtagccagt tccggattat ccagcagcag ctgccgcact gcatcccggc
     2161 cctgacccag tttatccgcc ccatagctga accagcttcc gcttttttga acgatgccga
     2221 attcaacgcc catatcaatc acttctccca tttttgaaat gccttctccg aagatgatgt
     2281 cgaattcggc actgcggaac gggggggcaa ctttattctt cactaccttc actttaacgt
     2341 ggtttccgat ggcttcttcc ccgtctttta tctgcgccat ccggcggata tccaaccgta
     2401 cggaagcgta gaatttcaac gcatttcccc cggtggtggt ttccggattt ccaaacatca
     2461 ccccgatctt ttcgcggagc tggttgatga aaatacagat cgtattcgtc ttgctgatgg
     2521 tcgcggttaa cttccggagg gcctgggaca tcagtcgggc gtgtaacccc attttgctgt
     2581 ctcccatctc cccctccagt tcccctttcg gtaccagggc agccaccgaa tcgatgacca
     2641 caacgtccaa tgccccggaa aggatcaggc ggtctgcaat ctccagggcc tgttcgccat
     2701 aatccggctg ggagatcagg aggctatcca catccacccc cagtttgcgg gcatagttgc
     2761 tgtcgaatgc atgttcggca tcaatgatgg cgcacatgcc gccttttttc tgggcctccg
     2821 cgatcacatg ggtggcgatc gtcgttttac ccgaggattc cggaccatag atttcaacca
     2881 ccctgccccg gggaaggccg ccgattccaa gggccacatc caggccaatg gaaccggttg
     2941 aaaccacctc catcggttcg gtactttttt cattcatcat catcacactc ccttttccaa
     3001 agtccttgtc tattttatct atggtcagct taagcgcctt cagtttttct gggtttgaca
     3061 ttttttttat tttatcattc aaagtagtgg attccacaaa gctaggagaa atattttatt
     3121 cacactaata aatttagtaa attttcacta atttttttgg ttggcctaaa agaggccccg
     3181 gcaaaagacg gggccgataa tgagaaattc tgatctataa aactggtttc cgaagccggt
     3241 ggttggccgg cttcatttca caaagagaag gaaatccggc acggaagtca ataccattaa
     3301 tgtggtaatt ttgagcgcat gaaccgtatt gacagattat tcggcatcct cacgctcctt
     3361 caatctaaaa aatacgttac cggggaaaaa atcgcggaaa agttcagcat cagcgtccgc
     3421 tccgtttacc gcgacatcaa ggcccttaac gaacagggca ttcccgtaag ctttgagcct
     3481 taccggggtt attttgttgt gaacgggtat ttcctgcagc cggtttcatt cagcaccgaa
     3541 gaagccaacg ccctgttatt aatggagtcg atcgtttccg gatttgccga taaatccatc
     3601 aagctgaatt attccaacgc gctgaccaag gttaaagccg tgctccggtc ctcgcaaaaa
     3661 gattccatcg aattcctggc cgataatatc aagttccagg ccccgccctg ttttatccag
     3721 cacatggatt atttgtccga cattcaaaaa gccatcgccc ggcggctggt gctcgagatt
     3781 gaatatacca acctgcagaa ggaggtttca aaacgaaagg ccgaacccat cggactggtt
     3841 ttttatgcct tcaactggca cctgatcggc tggtgccatc tcaaaaaagg gtatcgggat
     3901 ttccgcgtct cccgcatcct gcgcctccat tgtacgcagg aaaacttcct caacaaaaag
     3961 cacatggaaa tgaatgagta tatgaagcag ctcccggtaa acttttaaaa atcttttttg
     4021 ccctgccata gggttgtcag tctccggagt ttactttgtt catcatcaaa aaaaacgatc
     4081 atgaacagta tcatcagcta ttttcagaat cagcttcagg aagaagccgt taccgcccgt
     4141 aaaatgctgg agcgggtgcc ggcagacaaa ctcgactggc agccacatcc aaaaagcatg
     4201 agcctggtgc ggctggccac gcatgttgcc gagatcccca actggatccc gatgataacc
     4261 gaatcggatg aactggactt tgccaaaggc gattattctc cccatcccat caacagcaac
     4321 aaggacctgc tggattattt tgaaaagaat gtgaaagacg ggaaagccgc gctggaaaag
     4381 gcttcagacg agatcctgga taaaccgtgg accatgcgta gcggggagga tgtgttcatc
     4441 cgctccacca agggagaagt gctccgcatg agcctttccc agatcattca tcaccgcgcc
     4501 cagctcggcg tatacctgag actgttagat gtcccgatcc cgggaagtta tggaccgagt
     4561 gcggatgaac aataaaagca gatggctgac ggcttcggac cggatctccg tccggagccc
     4621 gtccgccatt cagggtttgt actgaaaact ccggatcacc acttccaccg gctgctggtt
     4681 ttccggtccg tttcctccga atagccagaa atttatatgg accggcatgg caaggtggct
     4741 gatcgaggca ggtttggtaa aaacagaaga aaagatttcg tccttcgggt ccgtggagag
     4801 cccgtcgaag ctcgcacaga tcacggtatc cgcatttcgt ttgaaataat gcgtgctcag
     4861 gttggatttc agggaaaatt ctttcaccgt ggcactgtcc tttaatcctc tttttgccgg
     4921 ccagatggta tagttcaggt tgggataggc cggattgccc caacgcgcaa tttcaatgtc
     4981 catttcatcg agcccgccat tgcccgaata attaaagagg cccagcacga tattcttgtc
     5041 gaatttgtca attgccccct ccaccacgaa ctggtaggtg ccatattcaa aagactgcag
     5101 ggaagtgatt tcggcgcaat tccaccggcc cgagaccgga tctttt
//
LOCUS       DASYCU010000269         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5134736, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000269 DASYCU010000000
VERSION     DASYCU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5134736"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VGM24_05385"
     CDS             <1..1291
                     /locus_tag="VGM24_05385"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY4154832.1"
                     /translation="EIARALFQSVENILQHFQQQPPVLIAFSAAMHSLIAVNERGLPL
                     SKCIIWADNRAAGIASRLHQEQKAKGLYLKTGVPVHAMSPFCKLLWLKENDPSLFQRA
                     FRFIGIKEYIFFKLFGLYAVDSSIAAATGLMNLNSIQWDQEILEETGIGQERLSQIVS
                     VREIFSAPSLFPRLKNVPFVIGGSDGAMANLGTASATGDSLVVSIGTSCAVRIIVRGP
                     VTDTNMRSFCYHVKDDLYLLGGAGNNGAVVLQWLREKFLVAPENLPDLLALASPVKAG
                     SEGLIFLPYLLGERAPVWNENARGVLFGLTINHDRSHLVRAAMEAVILCVYAIGLSLL
                     EKRDIRQIDVTGGFARNEVWIQILSDAFNLPARVSETVENAAWGAVKLGMESLGIAPV
                     PEKENPKIFFPCAETHRLYHDQFKKSERLYNLLKTEF"
     gene            1322..2971
                     /locus_tag="VGM24_05390"
     CDS             1322..2971
                     /locus_tag="VGM24_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY4154833.1"
                     /translation="MKKIIRVCLGSFLLAAFGCHSRQPVDLIIRNATIYTVDSAFSKE
                     SAMAIKDGRIVALGDDPEILKSFKAPRELNAAGKFVYPGFIDAHAHFVSYGSSLFQVD
                     LTGSASWAEVMLRVRKFVSLHPGLAWIQGHGWDQNRFPGKDFPSNDSLNILFPDRPVL
                     LARIDGHAAIANAKALALSAIRPGETINGGTVETAHGKLTGLLIDNAVGLVSEHIPAP
                     SAADYCRWLMAAQDRCFAQGLTGITDCGLMYDEVALIDSLQKADRLKMKLYVMLSDDP
                     ANYRRYLKTGPYKTPRLFVHGFKLYADGALGSRGACLLRPYQDKPGWYGFLLSNKEHF
                     DSVARRLSTTSFQMCTHAIGDSGNRMILTVYNKYLNGKNDRRWRIEHAQVIDKKDFDL
                     FGRSSIIPSVQPTHATSDMYWAEERLGPERIKSAYALKKLLDQNGWLPLGTDFPVEDI
                     SPFKTFHSAVFRKDANHYPETGFQPENALTREEAIRGMTIWAAKADFLEKETGSLEPG
                     KKADFILLDRDLMTAGEPEILNIRVLATYSDGQPVYQAVPR"
     gene            3136..3666
                     /locus_tag="VGM24_05395"
     CDS             3136..3666
                     /locus_tag="VGM24_05395"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY4154834.1"
                     /translation="MDINKIYKPKGFCPAFYKGYLDLVPDDGNLLIHLQDIFADTEEL
                     VYELPPEKLAYVYAAGKWTIKDILVHLCDAERVFIYRALRFARCDETPLPGFDEEVFA
                     KHTRAAEREIADIIREFSLVRESSIAFIESLNEEALERKGTANNNPLTVGLILNVIYG
                     HHKHHINIIHERYLNG"
     gene            3673..>4227
                     /locus_tag="VGM24_05400"
     CDS             3673..>4227
                     /locus_tag="VGM24_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154835.1"
                     /translation="MEKDKKRISIGIDMDGVLADTQTHFIKYYENETGIRIDPGSMLG
                     IPENEAFPDKTAVQRFVRIAGFFRSLPLIPGSVRAVKMLMKDFEVYIVSAAMEFPLSL
                     FEKKEWLEEHFPFISWKNIVFCGDKNIIRTDYLIDDHLKNLDYFPGIPLMFTASHNIH
                     HTHHQRLNNWEEILAYFRKVKETPA"
BASE COUNT         1047 a          999 c         1137 g         1044 t
ORIGIN      
        1 agaaattgca cgtgcgcttt ttcagtctgt ggaaaatatt cttcagcatt ttcagcaaca
       61 gccaccggtg ctgatcgcct ttagtgcagc catgcacagc ctgattgcgg tcaatgagcg
      121 gggcctgccg ctttcaaaat gtatcatatg ggcggataac cgcgctgcgg gaattgcttc
      181 ccggcttcat caggaacaaa aagcaaaagg actttacctt aagaccggcg ttccggttca
      241 tgccatgtcg cccttctgca aactgctctg gctgaaggaa aatgacccgt ccctttttca
      301 acgtgccttc cggtttattg gcatcaagga gtatattttt tttaaattgt tcggccttta
      361 cgcggtcgat tcctctattg ccgcggccac ggggctgatg aacctgaaca gcattcagtg
      421 ggatcaggag atcctggagg aaaccggaat agggcaggag cgtctttcac agattgtatc
      481 agtccgggag attttcagcg ctccttccct atttccccga ttgaaaaacg ttccttttgt
      541 gatcggtgga agcgatggtg cgatggcaaa tctgggaacg gcttccgcaa ccggtgattc
      601 gctggtggtg agtatcggca ccagctgcgc tgtacggatc attgtccggg ggccggtaac
      661 ggacaccaat atgcgaagct tttgttatca tgttaaggat gatctatacc tgctgggcgg
      721 ggcaggcaac aatggcgcgg ttgttcttca gtggctcagg gagaaatttt tagttgcacc
      781 ggaaaattta cctgatctgc tcgccctggc ttctccggtg aaggcgggca gtgagggcct
      841 gatctttctg ccctatcttt tgggtgaaag ggctccggtc tggaacgaaa acgcccgggg
      901 agtgctgttt ggactgacca taaaccacga ccggtcgcac ctggtacggg ctgccatgga
      961 ggcggttatt ctttgcgttt acgcgattgg gttgtccctg ctcgaaaaaa gagatatccg
     1021 gcagattgat gttaccggtg gctttgcccg gaacgaggtg tggatccaga tcctgtccga
     1081 tgccttcaat cttccggcaa gggtttctga aaccgttgaa aatgcggcct ggggcgcggt
     1141 aaagctgggt atggaatccc tggggattgc tccggtcccg gagaaagaaa atccaaagat
     1201 attttttccc tgcgcggaaa cccaccggtt gtatcatgat caatttaaaa aatctgaaag
     1261 attatacaac ctgttgaaaa ccgaattttg atccacgccg ttcctttaca acaaaaatga
     1321 tatgaaaaag ataatccggg tttgccttgg gtcttttttg ctggcagcct ttggctgtca
     1381 ttcccggcaa cccgttgacc tgatcattcg aaatgccact atctataccg tggattctgc
     1441 gttcagcaag gagtcggcca tggccataaa ggacggccgg atcgtcgcgc tcggcgatga
     1501 cccggaaatt ctcaaatcct ttaaagctcc ccgcgagctg aatgccgccg ggaaatttgt
     1561 ctatcccggc ttcattgacg cccacgccca ttttgtttct tatggaagca gtttgtttca
     1621 ggttgatctt accgggtccg ccagttgggc cgaagtgatg ctgcgcgtaa gaaaattcgt
     1681 atctcttcat ccgggactgg cctggattca ggggcatggc tgggatcaga accggtttcc
     1741 cggtaaggat ttcccatcca atgattccct gaatatcctg tttccggaca gaccggtttt
     1801 gctggcccgg atagatggtc atgctgccat agccaatgca aaagcgctgg ctctttcggc
     1861 aatccgtccc ggcgagacga ttaacggcgg cacggtggaa acggctcacg ggaagctaac
     1921 cgggttgctg atcgataatg ccgtgggatt ggtcagtgag catattccgg ctccatcggc
     1981 ggcggattat tgccgctggc tcatggcagc acaggaccgc tgctttgccc agggacttac
     2041 cgggattacc gattgcggat taatgtatga tgaagtagcg ctgattgatt ctttgcaaaa
     2101 agcagaccgt ctcaaaatga aattgtatgt gatgctcagc gatgatccgg cgaattaccg
     2161 ccggtacctt aagaccggtc cgtacaaaac accaaggctt tttgtacatg gatttaagtt
     2221 atacgcggac ggcgcattgg gaagccgcgg tgcctgtctg ctccggcctt atcaggataa
     2281 gcccggatgg tatggctttc tgctgagtaa taaagaacac ttcgattccg ttgccaggcg
     2341 gctgagcacg acctcttttc aaatgtgcac acacgccatc ggagacagcg gcaaccggat
     2401 gatcctgacg gtttacaata aatatttaaa cgggaagaat gatcgccgct ggcggataga
     2461 acatgcgcag gtcatcgata aaaaagattt tgacctgttt ggcagatcaa gcatcattcc
     2521 ttccgtgcaa cctacgcatg ccacgagcga catgtattgg gctgaagaac ggctgggccc
     2581 ggaaagaata aaatcggctt acgcgctgaa gaaattattg gatcagaacg ggtggcttcc
     2641 gctgggaacg gattttccgg tggaagatat ttctcctttc aaaacatttc attctgccgt
     2701 attccggaag gatgccaacc attatccgga aacgggattt caaccggaga atgcattgac
     2761 gcgggaagaa gcgatccggg gaatgaccat ctgggccgcg aaagcggact ttctcgaaaa
     2821 ggaaaccgga agcctggaac ccgggaagaa ggcggacttc atcctgctgg atcgggacct
     2881 gatgactgcg ggggaacccg aaatactgaa tatccgggtt ttggcgactt attcggacgg
     2941 ccagccggtt tatcaggccg tcccccggtg actgctctca gaattttctc agggccctcg
     3001 gttgatccgt ataccattcc cgtggaaaaa taaaaaaggg gcccggcata attttttagt
     3061 gaacggtaag gaggagggac cggatcggga tttcgtccgg atgtttctta catttgaaat
     3121 ctaaagcgga gaaaagtgga cataaataaa atatataagc ccaaaggatt ttgcccggct
     3181 ttttataagg gatacctgga cctggttccc gacgatggca acctcctgat ccacctgcag
     3241 gatatttttg ccgatacgga agaactggtt tatgaacttc caccggagaa gctggcctat
     3301 gtttatgcgg cggggaagtg gacgataaaa gatatcctgg ttcacctctg tgatgcggag
     3361 cgcgttttca tttaccgggc gctgcggttt gcccgctgcg atgaaacgcc gttacccgga
     3421 tttgatgaag aggtgttcgc aaaacacacc cgggcggcag aaagggaaat cgctgatatc
     3481 atccgggagt tcagcctggt cagggaatcc agtatagctt ttatagaatc cctgaatgaa
     3541 gaagcgctgg aaagaaaggg aacggccaat aacaacccgc ttacggtcgg cctgatcctg
     3601 aacgtgatct acggccatca taagcatcat atcaatatca ttcacgaaag atacctgaac
     3661 ggataaagcg ccatggagaa ggataaaaag cggatttcaa tcggtattga tatggatggc
     3721 gtacttgccg atacacaaac ccatttcatc aaatattatg agaacgaaac ggggataaga
     3781 atagatcccg gatccatgtt gggcattccg gaaaatgaag ctttcccgga caagacggcg
     3841 gttcagcggt tcgtgcggat agctggtttt ttcagaagcc tgcccctgat tcccggaagc
     3901 gtccgggccg tgaaaatgct gatgaaggat tttgaagtat acattgtatc agcggcaatg
     3961 gaatttcccc tgtcgctgtt cgaaaaaaag gaatggctgg aggagcactt tccatttatt
     4021 tcctggaaga atattgtttt ttgcggcgac aagaacatta tccgaacaga ttatctgata
     4081 gacgaccacc tcaagaacct ggattatttt cccggcattc cgctgatgtt caccgcttcg
     4141 cataatattc accatacgca tcaccagcgc ctgaataact gggaggaaat tctggcttat
     4201 ttcaggaaag taaaagaaac accggcg
//
LOCUS       DASYCU010000270         6499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2815256, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000270 DASYCU010000000
VERSION     DASYCU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2815256"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VGM24_05405"
     CDS             <1..1774
                     /locus_tag="VGM24_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4154836.1"
                     /translation="STNEFRGDPYAEFNNGFGSFNTWKNTLKAGTGLIGKHFTVDMRL
                     SRISSNGYIDRASSDLKSAYFSVAWLGEKTSLRFNLLTGAEKTYQAWNGVPEAKLYGD
                     LSALHEHYKNNSGYSGALYNTISDSLNLYQSDPRRYNYFTYKNQTDNYKQDHYQAFLN
                     HQFSGFLSANLAAFLTRGKGYYEEYKNGAAYADYGLPDQMVGDTLISNTDLIRQKWLD
                     NFFYGGIFSLQYKNQGTELTLGGGWDRYDGKHEGNIIWADRGGIPENYPYYHEPARKT
                     DFNVYAKWQQKLGSYFSSFADLQFHQIRYRIDGFDDNPALHISNQYFFLNPKAGFSYT
                     RGPWQGYISYALARHEPNRDDFEAGLTQQPKPETLHDFEIGWGKRLPHYNWSITGYYM
                     LYRDQLILTGKINDVGSYTRTNTPRSYRLGLELQGALKPVKWFQASGNLTLSRNKVVD
                     YTEYIDDYDNGGQKTNTYPESDIALSPNMIAAANLSFYPVPRVEIGLPAKYVGLSYLD
                     NSESDQKKISAYYVQQLRVIYSPKIESLKELNIAFQLNNLFNKRYEANGYTYGYYSGG
                     KLVNENFLFPQAGRNFMLSLNIRI"
     gene            complement(1775..2083)
                     /locus_tag="VGM24_05410"
     CDS             complement(1775..2083)
                     /locus_tag="VGM24_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY4154837.1"
                     /translation="MQRISVQEVHERLAKGESLNLVDVREPAEHAEFNIGGFLIPLGK
                     IRQMQVDELEDLKSRELICYCRSGNRSGQACQLLGMMGFSNTKNLEGGMLAWREKIRR
                     "
     gene            complement(2109..2918)
                     /locus_tag="VGM24_05415"
     CDS             complement(2109..2918)
                     /locus_tag="VGM24_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY4154838.1"
                     /translation="MFRFLTSKPLWVNMLAGLILLILLLLLFLGSLDIITHHGKTMKI
                     PSVIGQSIEAARKTLESQGFEVQIQDSIYNDTLPPLQVIKQFPEPDNLVKVNRTIYLT
                     ITQAVAPKVDMPNLLSMTFRNAEMVLHRYGLKLGDTVFKPDFARNSVLDQLYKGVSIK
                     PGSKIQQGSAVTLVLGNGVGMEFVVPDLFGLTYREARSALSESGLIIGSIVPDADVQD
                     TVNAFVYRQNPGRFDYEGKLNHIRQGQIIDIWLSAQKRERIVDSSAVKPAQ"
     gene            complement(2953..3951)
                     /locus_tag="VGM24_05420"
     CDS             complement(2953..3951)
                     /locus_tag="VGM24_05420"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY4154839.1"
                     /translation="MKRVIAFVTGGYSGEAEVSYKSAITIEAQIDKTKYEVYRIDITP
                     SAWFHATGDGKKIPVDKNDFSLQIDGRKINFDAVLIGIHGTPGEDGKLPGYFDLMKLP
                     YTSCDAATSALTFNKRYTVAVAAFSGIPVARSLHLFKQDRPGASEVLEKLKLPVFVKP
                     NNGGSSIGMSKVNKPEEMAAALEKALKEDDQVLVEEFIQGREFTIGVFRSGAEILTLP
                     FTEVKTTKDFFDFEAKYSPGFTEETTPAVVDESIAEKVRNTAKKIYRIFNCQGVIRID
                     MIYNEAKGEPFLLEINTVPGQTEASVVPQQVRAMGWTLNQFYSALIEDALSRAGKP"
     gene            complement(3978..4325)
                     /locus_tag="VGM24_05425"
     CDS             complement(3978..4325)
                     /locus_tag="VGM24_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY4154840.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGVEWAIADGTSVKGAYT
                     LMDGTVIDAGQRNEPVSVDTYYIVTNKCTECQGFHEEPQCASVCPVDVCIPDEMYRET
                     VEELLAKKDKLHL"
     gene            4384..5385
                     /locus_tag="VGM24_05430"
     CDS             4384..5385
                     /locus_tag="VGM24_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEY4154841.1"
                     /translation="MDLENRISLMLELAKMMKSRDPQWMEAKTRACQINPWFTPGFID
                     LAVENIVSCYLQRDLLEQWTRSAGIPGEQKDPRQIGVVMAGNIPLVGFHDFLSVFISG
                     HRQLIKLSSKDRELMTAILQFIHLARPETETAIQTAEMLKGCDGYIATGSNNTARYFD
                     YYFGKFPSLIRRNRTSAAILNGKESREELENLADDVHRYFGLGCRNVTKIFVPENYDF
                     VPLLEAFRKYHWMTEQHRYKNNYDYRLSLAILNKQYYMTNESILLLEEESLFSPISVL
                     HYAFYQPGNPVIAADLSDSIQCLVGKDFIPFGKSQQPSLTDYADGSDTLLFLRNLNG"
     gene            5550..>6499
                     /locus_tag="VGM24_05435"
     CDS             5550..>6499
                     /locus_tag="VGM24_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY4154842.1"
                     /translation="MKFKQVAGIVFISAATTLASLWGYDHFSGGKTYFYTQDSTKIPA
                     NYAKFFDGEKNAAGPADFTEAASAAIPATVHIKTKTVRTASNNLPRSNPFSDLFGIDP
                     NDFFGDRGRSLPEMGSGSGVIISDDGYIVTNNHVVDGADEVTVTLSNKKSFKAKVVAT
                     DPSTDLAVVKVDAKGLPFLLYGNSDDIKVGQWVLAVGYPLSLETTVTAGIVSAKGRSI
                     DINSRQSQSPVESFIQTDAAVNPGNSGGPLINPQGQLIGINSAIASPTGSYAGYSFTI
                     PVNIVKKVVTDLIKYGTTQRAYLGIEYARDNLSDDLKKQEG"
BASE COUNT         1669 a         1624 c         1566 g         1640 t
ORIGIN      
        1 ttcaaccaat gaattccggg gagatcctta tgcggaattc aataacggct tcggttcctt
       61 caatacctgg aaaaatacac tgaaagccgg aacggggctg attggaaaac atttcacagt
      121 cgacatgcgt ttgtccagga tcagcagcaa tgggtatatt gaccgtgcct ccagcgacct
      181 gaaatccgct tatttttcgg tggcctggct cggcgagaaa acatccctcc ggttcaacct
      241 cctgaccgga gcggaaaaaa cataccaggc ctggaatggg gtgccggaag ctaaattgta
      301 cggtgatttg tcggcgctcc acgagcacta taaaaataat tccggatatt ccggggcatt
      361 gtataatacc atttccgatt cccttaacct ctaccaatcc gatccgcgca ggtataatta
      421 tttcacttat aaaaatcaaa cggacaatta caaacaggat cattaccagg cttttctaaa
      481 ccatcagttt tccggtttcc tttccgcgaa tctggccgcc ttcctgaccc gcggcaaggg
      541 ctattatgaa gaatataaaa acggcgcggc gtatgcggat tatggcctgc cggatcagat
      601 ggttggagat acgctcatca gcaataccga ccttatccgg caaaaatggc tggacaattt
      661 tttttacggc ggtatctttt ctttacaata caagaatcag ggaacggaac ttacgctggg
      721 cggtggctgg gaccggtacg atggtaaaca cgaaggaaat atcatctggg cggaccgcgg
      781 cggcattccg gaaaattatc catactatca cgaacccgca cgaaaaacag atttcaatgt
      841 atatgccaaa tggcaacaga agctcggcag ctatttcagc agttttgccg atctgcagtt
      901 ccatcaaatc cggtaccgga ttgacggctt cgacgataat ccggcgcttc atatttcaaa
      961 tcagtatttt ttcctgaatc cgaaagccgg tttttcctat accagaggac cctggcaggg
     1021 atatatatct tacgcgctgg cccggcatga accgaaccgg gatgattttg aggcgggact
     1081 cacccagcag ccgaaacccg aaaccttaca tgattttgaa atcggatggg gaaaaaggct
     1141 tccccattat aactggagca tcaccggtta ttatatgctg taccgcgacc agcttatcct
     1201 caccggcaaa ataaatgatg tgggttccta taccaggaca aataccccgc ggagttaccg
     1261 gctgggcctc gaattgcagg gcgcgctaaa gcccgttaaa tggtttcagg cttccggaaa
     1321 tctgacgctg agcagaaaca aggtggtaga ctataccgag tatattgacg attacgataa
     1381 tggagggcaa aaaacaaaca catacccgga atcggatatt gctctttcgc ccaatatgat
     1441 tgcagctgcg aatctgagct tttatcccgt tccccgggtg gagatcggcc ttccggccaa
     1501 gtatgtcggc ctctcttatc tggataattc ggaaagtgat cagaaaaaga tcagcgccta
     1561 ttatgtacag cagttgcggg tgatctattc tccgaaaata gaatccctga aggaattgaa
     1621 tatcgccttt caattgaata acctgttcaa taaaagatat gaagccaacg ggtataccta
     1681 cggatattat tccgggggaa agctggtcaa tgaaaatttc cttttcccgc aggcaggccg
     1741 gaattttatg ttgagcctga atatccggat ctgatcatct cctgatcttt tccctccagg
     1801 ccagcatgcc gccttccagg tttttggtat tcgaaaatcc catcatgccg agtagctgac
     1861 aggcttgtcc gctccggttt ccgctccggc aataacagat gagctcccgg cttttcaggt
     1921 cctcgagttc gtctacctgc atctgtctaa ttttacccag ggggatcagg aagcccccga
     1981 tattgaactc cgcatgttcg gctggttcgc gcacatccac caggtttaga gactctccct
     2041 ttgcgagacg ttcatgtact tcctgaaccg agatcctttg catgtaaatt tattttgaac
     2101 aagaataatc actgggcagg cttcaccgcg ctgctgtcca cgatccgctc ccttttctgt
     2161 gcgctcagcc agatatctat tatctgtccc tggcggatat gattcagctt tccttcataa
     2221 tcaaaccggc ccggattctg acggtataca aacgcattga cggtgtcctg cacatccgcg
     2281 tccggaacga tagaaccaat gatcaggccg ctttcactca aagcggaccg ggcttcccgg
     2341 taagtgaggc caaacagatc cggtactaca aattccattc ccactccgtt gcccaaaacc
     2401 agggttaccg cacttccctg ctggatcttg cttccgggtt tgatgctaac ccctttgtac
     2461 aattggtcca ggaccgagtt acgggcaaaa tcgggcttaa aaaccgtgtc gccaagtttt
     2521 agtccgtacc ggtgaagcac catttccgca ttccggaagg tcatgctcag gagattcggc
     2581 atatccacct tgggggcgac cgcctgggta atggtcagat agatggtccg gtttaccttg
     2641 acgaggttgt ccggctcggg gaactgcttg atcacctgca gcggaggcag ggtatcatta
     2701 tatatggaat cctggatctg gacttcaaaa ccctgggatt ccagggtttt cctggctgct
     2761 tctatcgatt gcccgatcac cgaaggaatt ttcattgttt tcccgtgatg ggtaataata
     2821 tccagcgaac ccagaaaaag gaggagcagc aaaataagca ggataagccc cgctagcatg
     2881 tttacccaca agggtttact ggtgagaaat cggaacacaa tcagaattta agttgaaaga
     2941 tagttattat attcaaggct tcccggcccg ggacagtgcg tcttcaatca acgctgaata
     3001 gaattgattt agtgtccacc ccatggcccg gacctgctgc ggcactacgc tggcttccgt
     3061 ttgtccggga acggtattga tttccagaag aaagggttct ccctttgctt cattgtatat
     3121 catgtcgatg cggatcaccc cctggcaatt gaatatccgg tatatttttt tggcggtgtt
     3181 ccgcactttc tccgcaatgg attcatctac gacagccggc gttgtttctt ccgtgaatcc
     3241 gggcgaatat tttgcctcaa aatcaaaaaa gtccttggtt gtttttactt cggtgaacgg
     3301 cagggtcagg atctccgcac ccgaacgaaa gacgccgatc gtgaattccc tgccctgaat
     3361 gaattcctcc accagcacct ggtcgtcctc tttcagggcc ttttccaggg cggcagccat
     3421 ttcttccggc ttattcacct tggacatacc gatactcgaa ccgccattat tcggttttac
     3481 aaaaacgggc agctttagtt tttcaagtac ctcggaggcc ccgggcctgt cctgtttgaa
     3541 gagatgcagg gagcgggcca ccggtatccc ggaaaacgcg gctaccgcaa cggtataccg
     3601 tttattaaaa gtcaaggcgg aagtggcggc gtcgcaggag gtataaggaa gtttcatgag
     3661 gtcgaaatac ccgggaagtt ttccatcctc ccccggcgtg ccgtgaatac cgatcagcac
     3721 ggcgtcgaaa ttgatttttc tgccgtcgat ctgaagcgaa aaatcatttt tgtcaaccgg
     3781 gatcttcttt ccgtccccgg tggcatggaa ccaggcggaa ggcgtaatat cgatccggta
     3841 gacctcatat tttgttttat caatctgggc ttcgatggtg atggcgcttt tatacgacac
     3901 ttcagcctca ccggaataac cgccggtaac gaaagcaatg acccttttca ttatttggat
     3961 ttaagcattt gccggaatca gagatgaagt ttgtcttttt ttgccagcag ttcttccact
     4021 gtttcccggt acatttcatc cggaatacaa acatccaccg ggcaaacgga ggcacactgg
     4081 ggttcttcgt gaaaaccctg acattcagtg catttattgg taacaatata ataagtatcc
     4141 acgcttaccg gttcatttcg ctggccggcg tcaataacgg ttccatccat cagggtataa
     4201 gcccctttta cggaagtgcc gtctgcaata gcccattcca cacctccttc gtaaatcgcg
     4261 ttattcgggc attccggttc gcaggcgccg caattgatgc attcttcggt tatcttgatc
     4321 gccataatat tgttattagg agttgattag aactaatttc gcaggtcaaa gataatctga
     4381 aatatggact tggagaaccg gatcagcctg atgctggaat tggcaaaaat gatgaagtca
     4441 cgggacccgc agtggatgga agcaaaaacg cgggcctgcc agataaatcc ctggttcaca
     4501 cccggattta ttgatctggc ggtggaaaat attgtaagct gctatttaca aagagacctg
     4561 ctcgagcaat ggacccggtc cgccgggatc cccggagaac aaaaagatcc aaggcagatc
     4621 ggggtggtca tggccggcaa tattccgctg gtgggttttc atgatttttt gtctgttttc
     4681 atcagcggcc accggcaact gatcaaactg tcttcaaagg atcgggaact catgaccgca
     4741 atcctgcaat ttatccattt ggcccgcccg gaaacggaaa ccgcaattca aacggcggaa
     4801 atgctgaagg gctgcgatgg atatattgcc acgggcagca acaatacggc ccgttatttt
     4861 gattattatt ttggaaagtt tccaagcctg atccgccgca accgcacctc ggccgccatt
     4921 ctgaacggaa aggaaagccg ggaggaactg gaaaacctgg ccgacgacgt gcaccggtac
     4981 ttcggactgg gctgccggaa cgtgactaaa atttttgttc cggaaaatta tgatttcgtg
     5041 cctttgctgg aggctttccg gaaataccac tggatgacgg agcagcaccg ctataagaac
     5101 aattatgatt accggctttc tctggccatc ctgaacaagc aatattatat gaccaatgaa
     5161 tccatcctgc tcctggaaga ggaatctctt ttttcaccca tcagcgtact tcattatgct
     5221 ttttaccagc ccgggaatcc ggtcattgca gcggaccttt cagacagcat ccaatgcctt
     5281 gtggggaagg acttcatccc ttttggaaaa agtcagcagc cttccctgac cgactacgcc
     5341 gatgggtccg acaccctgtt atttctccgg aatttgaatg gatgaccggc attgacagct
     5401 aagttttctt ttgtaaattt gggaaccttc atctacgaag gtcttagtaa attatttgtt
     5461 aaactgaagg ttaccgatta acggaacgtc caactcggtg ttattttgtt gttgaaaagg
     5521 gatcaatcag actagaaaaa aataaaaaaa tgaaattcaa acaagttgca ggcattgttt
     5581 ttatcagtgc agccactacc ctggcgagtt tgtgggggta tgatcatttc tccggcggca
     5641 aaacgtattt ctacacacag gactccacca aaattccggc gaattatgcc aaattctttg
     5701 acggggaaaa gaatgccgcg ggacccgccg actttaccga ggcggccagc gcagccattc
     5761 cggccaccgt gcatatcaaa acaaaaacgg tcaggacggc cagcaataat cttccgagaa
     5821 gcaatccgtt ctccgatctt ttcggaatcg atccaaacga ttttttcggg gaccggggcc
     5881 ggtcacttcc tgaaatgggt tcgggttccg gcgtcatcat cagcgatgac ggatatattg
     5941 taaccaataa ccatgtggtg gacggggcgg atgaggtaac ggtcaccctg agcaataaaa
     6001 aatctttcaa ggcaaaggta gtggcaacgg atccgagtac ggatctggcc gtggtcaagg
     6061 tagatgccaa gggattgccc ttcctgcttt atggaaactc agacgatata aaggtcggtc
     6121 agtgggtgct ggccgtcggt tacccgcttt ctctggaaac aaccgttaca gccggaatcg
     6181 tcagtgccaa agggagatcg atcgacatta acagccggca gagccaaagc cctgttgaat
     6241 cctttataca aacagacgcg gcggtgaatc cgggaaacag cggagggccg ctgatcaatc
     6301 cccagggcca gcttatcgga attaattcgg ccatcgcatc gcctaccggt tcttacgcag
     6361 ggtattcatt taccattccg gtgaacatcg taaagaaagt agtgaccgac ctgatcaaat
     6421 acggaacaac acagagagct tatctcggca tcgaatatgc ccgcgacaac ctcagtgatg
     6481 acctgaagaa acaggaagg
//
LOCUS       DASYCU010000271         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3249812, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000271 DASYCU010000000
VERSION     DASYCU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249812"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..905
                     /locus_tag="VGM24_05440"
     CDS             96..905
                     /locus_tag="VGM24_05440"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY4154843.1"
                     /translation="MFDTQSFRPDPLLREVVDRYQLVKEAIETPYAEYCFLPKVSQQI
                     SFNLGPDKSVYDLDNQDYISSCNLIGQNDRICRIRIYNGMSRLIVNLKSLGWYKLFRI
                     PAPRFINRSCNLQHLLGNEIQLLHQQLLACNDFKKQIQLLDTFFMKRLFQQEGHYKNI
                     EGAVHRILELKGNISINRLEKEVFITRRTLERHFLEQTGLFPKMFARITRFSEAIKYL
                     EKNLKPNWPYLINCLGYCDQSHFINEFQHFAGCPPTHFYDQRVEFETAGLS"
     gene            982..1674
                     /locus_tag="VGM24_05445"
     CDS             982..1674
                     /locus_tag="VGM24_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY4154844.1"
                     /translation="MENINPKNGSPRRDFLGKLAAGAAAIGAAALVSPVQAVSGISAP
                     FKNADDPEAWLGRIKGKHRIAFDVTGPNEIMPFAWPKVFLMTNGAASKDCGVVVILRH
                     NAIGYTMGNELWEKYKFGEVFKIDDPATKAPAVRNIFWEPKAGDFSVPGIGNVDIGIN
                     QLQQDGVLFCACDVALTVFSTVLAGKMNMAAADVKKEWLAGLLPGIQVVPSGVWAVGR
                     VQEHGCSYCFAG"
     gene            1675..>1978
                     /locus_tag="VGM24_05450"
     CDS             1675..>1978
                     /locus_tag="VGM24_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEY4154845.1"
                     /translation="MKRNRIILITTGAILIAGGIIFLTGNKTGNKNIPAAGHYTSASK
                     NRDWTAPDSSSIPHTAEGDLIRYGRKLIVNTSYYFGPKGIISHLANGMNCQNCHLEA"
BASE COUNT          535 a          486 c          478 g          479 t
ORIGIN      
        1 atcccggaaa tttcttatct tccggggcta ctccggctga atccgtacct gctgaatccc
       61 catatatccg gtattccgat gggataaatt aacctatgtt tgatacccaa tctttcagac
      121 cggacccact actccgggag gtcgttgacc ggtatcagct ggtgaaagaa gcgatagaaa
      181 ctccatacgc cgaatattgc ttcctgccaa aggtcagcca gcagatctcg ttcaacctcg
      241 gccccgacaa aagcgtatat gatctggata atcaggatta catttcttcc tgtaatctga
      301 tcgggcagaa cgaccgaatc tgccggatca ggatttataa tgggatgagc cgcctgatcg
      361 taaatcttaa atcgctggga tggtacaaac tgttccggat acccgctccc cggtttatta
      421 accggagctg caacctgcag catttattgg gaaatgagat ccagttgctc catcagcaat
      481 tactggcctg caacgacttc aaaaaacaaa tccagctact ggatacattc ttcatgaaaa
      541 ggctgtttca gcaggagggc cattataaaa atatagaagg ggccgttcac cggatccttg
      601 aactaaaagg gaatatttcc attaaccggc tcgagaaaga ggtcttcatc acccgtcgca
      661 ccctggagcg tcattttctg gaacaaaccg gcttatttcc aaaaatgttt gcccggataa
      721 cccgtttcag cgaagccata aagtatctgg aaaaaaatct gaaacccaac tggccctatt
      781 taatcaattg cctgggatac tgcgatcaaa gtcatttcat aaacgaattt caacatttcg
      841 ccggctgccc gcccacccat ttttacgatc aacgcgtgga atttgaaacc gccgggctat
      901 cctagtattt ttttgtcact tttttgcaat gattcattcg gcagggattt tagcttagca
      961 tcctaaccaa accagaacaa catggaaaat atcaatccaa aaaacggcag ccccaggaga
     1021 gatttcctcg gaaaactggc tgccggcgcg gctgccatcg gtgctgccgc cctcgtatcc
     1081 cctgtacagg cggtttccgg gatatccgca ccgttcaaaa acgccgatga tccggaagcc
     1141 tggcttggcc ggatcaaagg aaaacaccgc attgcattcg atgttaccgg gccgaatgaa
     1201 ataatgcctt ttgcctggcc aaaggtcttc ctgatgacga acggagccgc atcaaaggat
     1261 tgcggggtgg tcgttatctt aaggcataac gcgatcggat atacgatggg aaatgaacta
     1321 tgggaaaaat ataaattcgg ggaagtattt aaaattgacg acccggcgac caaggcgcct
     1381 gccgttcgca atattttctg ggagccaaag gcgggcgatt tttctgttcc gggtatagga
     1441 aatgtggaca tcggtattaa ccagctgcag caggatgggg ttctgttttg tgcctgcgat
     1501 gtagcgctga ctgtgttcag cactgtgttg gcgggcaaaa tgaatatggc cgccgctgat
     1561 gtgaaaaaag aatggctggc cggtttatta cccgggatcc aggtggttcc ttccggcgtc
     1621 tgggcagtcg gccgggtaca ggaacacggg tgctcctatt gttttgcggg ctgaatgaaa
     1681 cggaaccgga tcattttaat aacaacaggg gcaatcctga ttgccggagg tattattttt
     1741 ctaaccggaa ataaaaccgg aaacaaaaat ataccggctg caggccatta tacctcagca
     1801 tcaaaaaacc gggactggac ggctcccgac agcagctcca ttccgcatac cgccgaaggc
     1861 gacctgatcc ggtatggtag aaaactgatc gtgaatactt cctattactt tggcccgaag
     1921 ggaatcatca gccacctggc aaatggtatg aactgtcaga attgccatct ggaagccg
//
LOCUS       DASYCU010000272         4472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3829532, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000272 DASYCU010000000
VERSION     DASYCU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3829532"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /locus_tag="VGM24_05455"
     CDS             <1..2097
                     /locus_tag="VGM24_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4154846.1"
                     /translation="YREKRVFFPQTFFVDTAFLRMFSYPMLAGNRQTALLKPNSAVLT
                     ESAARKLFGKEDPIGKTITHYAGDTLSFTVTGVMKDIPGNSQLQFDALMSFNTVYKPD
                     WMNGWGGNWLNTYLELAPHTNVAAMEKRFPAYLKKHMTGDGEGWKFYVLFLLPFKDVH
                     SNAADIGLDYINFQKFDKNYTNIFMVIALIVLLIACINFMNLSTARSSERAKEVGIRK
                     SIGAQRFQLGFQFLGETVLLALIALCIALALVFLALPYIENLSQRNLRPLFFQHPGLS
                     IAVLLGTIGVGLISGLYPAIYLSSFQASKVLKGSVQTGNKKGNFRNILVVGQFVSAIF
                     LIIATIFVFRPFNFMQHQDPGFVRDQVVTIPLDDVTSRKYDLLKEELSANSLVEGVTA
                     AQDQLGSHLDQSGIGFQGDGPLRNLTSTRLIVDPDYLRLYQIKLAYGRNFSNEKQANG
                     KEYIINEALARELLKDNPKAGISSLLGRHFGFDSAGHLLGNIVGIAKDFNFNSLHYKI
                     ETMFLFNQKDWGYSTVSVKINGGKAQESLAFIQSTWKSIFPDHPFEYQFLDDHFKEVY
                     RTDAQITQMVGILAVLAILISCLGLFGLASYSAERRIKEIGVRKVLGASIPDIVNMLS
                     RHFIKLVIVANLIAWPLAWFSVNRWMQDYAYRIPISWWVFLLAGVVALAIALVTVSLL
                     AAKAAMANPVDSLRTE"
     gene            2209..3471
                     /locus_tag="VGM24_05460"
     CDS             2209..3471
                     /locus_tag="VGM24_05460"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEY4154847.1"
                     /translation="MEKISERIVLFNKDRLSRIVQLKYEAMAENLFRFYRGTNHLFYE
                     DLQKAASLPESPLAWICGDLHLENFGSYKSDNRLVYFDLNDFDEGILAPAAWELARIL
                     TSIFIAFESLKIGQEKALKMARLFLKSYSAKLAGGKPDYIEPQTAKGIVCEFLTAVSR
                     RRQREILKKKTVLKKNRLEILLDDPRHFELKKELKKRLIQHITHWLKNDGQSPYNYKV
                     MDAVFRLAGTGSVGVKRYAFLLKSINKEGEKYLLIDMKQSKPSSLLPFLQIPQPAWSS
                     EAERILDIQKRMQNRSPALLSTTEFEGDSFVIQEIQPTKDSINFKLIKKEYRNIYQVI
                     DDMAMLTASSVLRSSGRQGSAITDELISFGRQDHWQEPLLQYAVQYALEVKKNYLLFL
                     KDYIQGALARSGEDPQTAGILAAGQKID"
     gene            complement(3468..3860)
                     /locus_tag="VGM24_05465"
     CDS             complement(3468..3860)
                     /locus_tag="VGM24_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4154848.1"
                     /translation="MNNTTNKTRHSASECTQSLAAVGDAMYAIGGKWKLRIIIALQDG
                     SKRFNELQRTVEGISARVLSNELKELEMNGFVKRKIYDQTPVVVQYEITDYSHSLQEV
                     LDSLRDWGTRHRARIRKEMKKQRKAAEG"
     gene            3965..4357
                     /locus_tag="VGM24_05470"
     CDS             3965..4357
                     /locus_tag="VGM24_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4154849.1"
                     /translation="MKTNKVLYWTATIIVAGMMVYSAYAYLTQAAIEQAFHHLGFPGY
                     FRIELAVAKLLGAILLLAPVAGRIKEWVYSGFFITFVSAFIAHTASGDPLQYRMMPLI
                     FLALLLASYISYQYLRRTETRSAFVQPA"
BASE COUNT         1082 a         1151 c         1103 g         1136 t
ORIGIN      
        1 taccgggaaa aaagagtttt tttcccgcag actttttttg tggatacggc ctttctgcga
       61 atgttctctt atccgatgct ggctggaaac cggcagacgg ccttgcttaa gcccaacagc
      121 gcggtactca cggaatccgc cgcgcggaaa ttattcggaa aggaagatcc cataggaaaa
      181 accattacac actatgccgg cgataccctg agtttcaccg taaccggagt tatgaaagat
      241 attccgggta attcccagct gcaattcgat gccctgatgt cctttaatac ggtctataaa
      301 cccgactgga tgaatggctg gggcggcaac tggctgaata cttacctgga gctggccccg
      361 catacgaatg tggctgccat ggagaagagg tttccggcct atcttaaaaa acatatgacc
      421 ggtgacgggg aaggatggaa attttacgta ttgttcctgc tcccgttcaa agacgtacat
      481 tccaatgcgg cggatatcgg cctggattat atcaactttc aaaaattcga taaaaattac
      541 accaatattt ttatggtgat cgcgctgatc gttttgctaa tcgcctgcat caatttcatg
      601 aacctgtcca ctgcccgttc gtcggaaagg gccaaagagg tcggcatccg taagtccatc
      661 ggcgcccaac gcttccagct gggcttccag tttctcggag aaacggtttt actcgccctt
      721 attgcccttt gcattgccct cgcactcgta tttctggcgc ttccttatat cgaaaacctg
      781 agccagcgga atctgcgtcc tttgtttttt cagcatccgg gcctgagcat cgcggtcctg
      841 ctgggaacga tcggcgtggg cctgatctcc ggactttacc ctgccatcta tctttcttct
      901 ttccaggcct cgaaagtatt gaagggatcg gtgcagaccg ggaataagaa aggaaatttt
      961 cgcaatatcc tggtggtcgg acaatttgtt agcgcgatat ttctgatcat cgccaccatt
     1021 tttgtttttc gcccgttcaa tttcatgcag caccaggatc cgggattcgt aagggaccag
     1081 gtggtcacga ttccgctcga tgacgtcacc tcccgaaagt acgacctgct taaagaagaa
     1141 ttgtcggcca actccctggt ggagggagtc accgccgcgc aggatcagct gggcagtcac
     1201 ctggaccagt ccggcattgg attccagggt gacgggcccc tgcgaaacct gacctccacc
     1261 cgcctcatcg tggatccgga ttatctgcgt ctctatcaga tcaaactggc ttacgggcga
     1321 aatttctcca atgaaaaaca ggccaatgga aaagaataca tcatcaacga agccctggcc
     1381 agggaattat tgaaggataa tccgaaggcc ggaatctctt ccctgctggg caggcatttc
     1441 ggttttgact ccgcgggcca tctcctgggt aatattgtgg gcattgccaa agattttaat
     1501 ttcaattcgc tccattacaa gatcgaaacg atgttcctgt tcaatcagaa ggattgggga
     1561 tacagtaccg tgtccgtaaa aatcaacggc ggcaaggcgc aggaatccct tgcattcatt
     1621 caatccacct ggaagagtat ttttccggat catcccttcg aataccagtt cctggatgat
     1681 catttcaagg aagtttaccg caccgacgcg cagatcacgc agatggtggg catactggct
     1741 gtgctcgcca tcctgatctc ctgtctcggt ctgttcggtc tcgcttccta ttccgcggaa
     1801 agaaggatca aggagatcgg ggttcgaaaa gtcttgggcg cttccatacc ggatatcgtg
     1861 aacatgctct cccgccattt tatcaagctc gtcattgtag ccaacctgat tgcctggccc
     1921 ctggcctggt tcagcgtcaa ccgctggatg caggattatg cttaccgtat tccgataagc
     1981 tggtgggtat tcctgctggc tggtgttgtt gcgctggcca ttgccctggt tacggtaagc
     2041 ctgctggctg cgaaggcagc gatggcaaat ccggtggaca gtcttcgaac cgaataaatt
     2101 ttacctatga tcaggaacca ttttctcccg gcatcaccgg cctgctgacc gccaatccgg
     2161 tgaacagcct gagggtggaa tagttgttgc tatcttcgga tattaaccat ggaaaaaatc
     2221 tccgagcgaa tcgtcctgtt caataaagac cgcctgtccc gcatcgtgca attgaaatac
     2281 gaggccatgg ccgaaaacct gttccggttt taccggggaa cgaatcacct gttttacgag
     2341 gatcttcaga aggctgcttc cctgcctgaa tcccccctgg cctggatatg cggtgacctt
     2401 cacctggaaa atttcggcag ttacaaaagc gataaccggc tggtatattt tgacctgaat
     2461 gattttgacg agggcattct ggcgccggcc gcctgggaac tggcccggat cctgacgagc
     2521 atttttatcg cttttgagtc cctgaaaata ggccaggaaa aagcattgaa aatggccagg
     2581 ctcttcctta aatcctattc ggccaagctg gccggcggca aaccggatta tatcgaaccc
     2641 cagacggcca aaggcatcgt gtgtgaattt cttacggccg tcagccggag aaggcaaagg
     2701 gaaatactga aaaaaaagac cgtcctgaaa aaaaacaggc tggagatcct gctggacgat
     2761 ccgcgacatt tcgaattgaa aaaggaattg aaaaaaaggc tgatccaaca tattacgcac
     2821 tggctgaaaa acgacggcca gagtccctac aattataaag taatggatgc cgtattccgc
     2881 ctggcgggaa cgggaagcgt aggggtgaaa cgctatgcct ttctcttaaa atcaataaat
     2941 aaagaagggg agaaatatct gctgatcgat atgaaacaat caaaaccctc ttcgctcctc
     3001 cccttcctgc aaatccccca accggcctgg agttccgagg cagaacggat cctggacatt
     3061 cagaaaagaa tgcagaacag gtctcccgca ttgctcagca cgacggaatt cgaaggcgat
     3121 tcttttgtaa tccaggaaat tcaacccacc aaagacagca tcaattttaa gctgatcaaa
     3181 aaagaatacc ggaacatata ccaggtgatc gatgatatgg ccatgctgac ggcctcttcc
     3241 gttttaagaa gctccggaag acaagggtct gcgataaccg atgaactgat ttcatttggc
     3301 cggcaggacc attggcagga gcccctgctg caatatgccg ttcaatatgc cctcgaggta
     3361 aaaaagaatt atttactatt tctgaaagat tatatccagg gcgcattggc ccgttccggg
     3421 gaagatccgc agaccgcagg gatcctggcc gccgggcaaa aaatagacta gccttcggcc
     3481 gctttccgct gttttttcat ttccttcctg atcctggccc ggtgccgcgt tccccaatcc
     3541 cttagggaat ccagcacttc ctgcagcgaa tggctgtaat ccgtgatctc gtattgcacc
     3601 acgaccggcg tttggtcata tattttccgc ttcacaaaac cattcatttc aagttccttc
     3661 agttcgttcg ataagacccg cgcggaaatg ccttccaccg tgcgctgcaa ttcattgaag
     3721 cgcttgctgc catcctgcag ggcgataatg atccgcagct tccattttcc gccgatcgca
     3781 tacatcgcgt ctcctacggc tgccagactt tgggtgcatt cacttgcaga atgccgggtt
     3841 ttgtttgtcg tattgttcat tttttccggg atgatgctaa ccaaaaggtt agtactaacc
     3901 ttttgtatac tgctaactat ttacaagcaa atgtatatag ttttgtgctt gcaaaaacag
     3961 aattatgaaa acgaataaag tgttgtactg gaccgcgacg atcatcgtgg cggggatgat
     4021 ggtctattcc gcctacgcct atcttacgca ggcggcgatc gaacaggcat ttcatcattt
     4081 gggtttcccg ggttatttcc ggatagaact ggcggtggcc aaactcctgg gcgcgatcct
     4141 gctgctggct cctgttgccg gcaggataaa agaatgggtc tattcgggtt tcttcatcac
     4201 cttcgtctct gcctttattg cgcatacggc ctccggagat ccgctgcaat accggatgat
     4261 gccgctgatc ttcctggcat tgctgctggc ttcttatatc agctaccaat acctgcgcag
     4321 gacggaaaca cgcagtgcgt ttgttcagcc cgcataatcc cggttaagga tggcggctgg
     4381 ggataatggg tgatggctgc gggataaccg gtgcctttgc aaactataca gcctgtatcg
     4441 gttccgtaca gggtcttccg gcaggggatc tt
//
LOCUS       DASYCU010000273         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4555734, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000273 DASYCU010000000
VERSION     DASYCU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4555734"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VGM24_05475"
     CDS             complement(<1..729)
                     /locus_tag="VGM24_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154850.1"
                     /translation="MEKKHILILTDWYEPGYKAGGPIQSCRHFIAAMHDRFRISVLTS
                     DRDIGDSTAYPGLPTGQWILREPGINIYYADVSTLDGRLMGDLILSRKPDFVYLNSMY
                     SYRFSILPLFLSWQKKIQTQIVLSPRGMLQQGALKFKAVKKKSFIFLLRRMHIPERIH
                     FHATDPQEKKDILRWFPKAGKVEMIPNFSSAVPSHGLPVEKVAGQLRCVYISRIIPKK
                     NLLFFLKLLNLVPDQIQLEFTIYGE"
     gene            complement(772..1641)
                     /locus_tag="VGM24_05480"
     CDS             complement(772..1641)
                     /locus_tag="VGM24_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4154851.1"
                     /translation="MLTRRQKIKYLCNSILALGQEVQCPFCKSMLYEEIDRKYWVTRL
                     MECKNCHLYYRFPVDKVEVNDAFYQTEYQESDAMASDLPDDETLRKEMESGFASNVKH
                     GARFLNLFNRLFPDRPTLRIVDYCCSWGYMAHQFKQAGHEVQGYEISRPRAAFGTEKM
                     GLNIYTREQDLKGNNDIFFSSHVIEHHPDIGGMLRLAEKILAPGGYFIAVSPNGSPPF
                     REKFPESFHLAWGKVHPNYLNGHFYQYIFRDRAHYIGSSPFNLKHIGPLRPKEMMVDD
                     LSGEELLVIARFG"
     gene            complement(1644..>2174)
                     /locus_tag="VGM24_05485"
     CDS             complement(1644..>2174)
                     /locus_tag="VGM24_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEY4154852.1"
                     /translation="LQETDQETFTKQLESKRSALIKLPFFSHVTAAEYLGYTQQTLLK
                     DTDQMSMANSLEIREPFFDQDLVEFLMSVPDHFKVPVYPKSLLVESLKPLLPDEIVHR
                     KKQGFVFPWNEWIKHELRDFCNTQIDRMCQRDFIRGGSLKTYWMRFLSGDPNIRWQEI
                     WLFVVLNYWMEKNGCE"
BASE COUNT          580 a          547 c          479 g          568 t
ORIGIN      
        1 ttcgccataa atggtaaact ccaattgtat ctggtccggc acgaggttaa gcagtttaag
       61 aaaaaaaaga aggttctttt tcggaatgat gcgtgaaatg tacacgcaac ggagctggcc
      121 ggcaaccttc tccacaggca atccgtgtga tggcacggcg ctggaaaaat taggaatcat
      181 ttccacttta cctgctttcg gaaaccagcg aagtatgtcc tttttttcct gaggatccgt
      241 ggcatgaaaa tgaatacgtt cgggaatatg catgcgcctg agcaggaaga tgaatgattt
      301 tttcttgact gccttaaact tcaatgcgcc ctgctgcaac atcccccggg gagaaagcac
      361 gatctgcgtc tgaattttct tttgccagct caagaacagc ggcaggatgc tgaaccggta
      421 cgagtacatg ctgttcagat atacgaaatc cggcttcctg gaaagaataa gatcgcccat
      481 caaacggcca tccagggtac ttacatctgc gtaataaata tttatccccg gctcccgcaa
      541 aatccactga cccgtgggaa gccccggata ggctgtactg tctccgatat ctcgatcgga
      601 cgtaagtacc gagatccgga agcgatcgtg catcgctgct ataaaatggc ggcaagactg
      661 tatcgggccg ccggccttat atcccggttc ataccagtca gtaagaataa gaatgtgttt
      721 cttctccaat ttgtaaactt taaagccatc aaaatccgga gtgccggcct tttacccgaa
      781 tcttgcgata accaataact cctcgccgga aagatcatcc accatcatct ccttcggccg
      841 aagtggtccg atatgcttaa ggttaaaggg actactgccg atataatggg cgcgatcccg
      901 aaaaatatac tggtaaaagt gcccgttcag ataatttggg tgcaccttac cccaggccag
      961 gtgaaaggat tccggaaatt tttccctgaa aggcggactg ccgttgggtg aaaccgcaat
     1021 aaaatacccc ccgggggcca ggattttttc agccagccgg agcatccccc cgatatcggg
     1081 atgatgttct atcacgtgag atgaaaaaaa tatgtcgttg tttcctttca ggtcctgttc
     1141 tcgggtataa atattgagtc ccatcttctc tgttccaaat gcggccctcg gtctggagat
     1201 ttcatatccc tgaacctcat gacctgcctg tttaaactga tgggccatat agccccagct
     1261 gcaacaatag tcaacgatcc gaagtgttgg ccggtcggga aacaggcggt taaagagatt
     1321 gaggaaccgg gcaccgtgtt tgacgttgct tgcaaaaccc gattccattt ccttccgaag
     1381 cgtctcgtcg tctggcaaat ccgaggccat ggcgtcggac tcctgatatt ccgtctggta
     1441 gaaggcatcg ttgacttcta ctttatccac cggaaaccgg taatagagat gacaattctt
     1501 acattccatc aatcgcgtaa cccaatattt tctgtctatt tcttcataaa gcatgctctt
     1561 acaaaaaggg cattgcactt cctgtcccaa agccaaaata gaattgcaca gatatttaat
     1621 cttttggcgg cgcgtcaaca taattattca catccatttt tttccatcca ataattcaac
     1681 accacaaaaa gccagatctc ctgccaccgg atattcgggt cccctgataa aaacctcatc
     1741 cagtaggtct tcagcgaccc gccgcgaatg aaatctcttt ggcacatgcg atcgatctgc
     1801 gtattgcaaa aatcccgaag ctcatgcttt atccattcat tccacggaaa gacaaatccc
     1861 tgtttttttc ggtgaacgat ttcgtccgga agcaagggtt ttaatgactc caccagtaga
     1921 cttttaggat aaacaggcac tttgaaatga tcgggaaccg acatcaaaaa ctcgacaaga
     1981 tcctgatcaa aaaacggctc ccggatttcc agagaattcg ccatgctcat ctggtcggta
     2041 tccttaagca gggtttgttg cgtatatccc agatattcag ccgcagtcac atgactgaag
     2101 aacggcagtt ttatcaacgc ggatcttttg ctctccaatt gttttgtgaa ggtctcctga
     2161 tcggtttcct gcag
//
LOCUS       DASYCU010000274         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3829680, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000274 DASYCU010000000
VERSION     DASYCU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3829680"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..1506
                     /locus_tag="VGM24_05490"
     CDS             445..1506
                     /locus_tag="VGM24_05490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY4154853.1"
                     /translation="MKKLLFITVLFCLAAGCGRKNSLHLFPGGNPLIQYIGRIDFSTA
                     ARPRFWNPGTYISIRFRGSSCDVLVNDEVSGGENHNYLEIILDNQKPERIKLTDRENN
                     IHIGDHLAAGDHSLVIVKNTEAGIGWLEFAGIRCEKLLPVKLPSRKMECIGNSITCGT
                     GSDTSVFACGQGQWYDQHNAWLSYGPLTARALDAQYHLTAVSGIGLIHSCCHMDVLMP
                     QVFDKIDTHGNKIKWDFNEYQPDVVTICLGQNDGAGDSVNFFNAYTGFIARIRKVYPR
                     TQIVALTSPMADEFLKNAMVRYLTTIVEYENRQHDPNIHLYVFSKRYHQGCGDHPLLS
                     EHREIAAELTAYIQKLMGW"
     gene            complement(1543..2016)
                     /locus_tag="VGM24_05495"
     CDS             complement(1543..2016)
                     /locus_tag="VGM24_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY4154854.1"
                     /translation="MKTSIGISDANRKTVAEQLAKLLADEFVLYTKTLNAHWNIEGPD
                     FHSVHLYFEELYEEAAKTVDSVAERIRQLGHYAPATLRDFLDLTHLTEKLKGGNTSLS
                     LIKNLLEDHESIIEFIRGNVDEFADAHKDAGTSDYITGLMEKHEKTAWMLRAHIK"
     gene            2125..2370
                     /locus_tag="VGM24_05500"
     CDS             2125..2370
                     /locus_tag="VGM24_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154855.1"
                     /translation="MEESFTITVLDKGIEKELAGRLRISTYTYQFLFEIGDSEIILER
                     DDEGNFRALGSQAVPGKKPDPSLVQTLIRAMEEIMKE"
     gene            2397..2639
                     /locus_tag="VGM24_05505"
     CDS             2397..2639
                     /locus_tag="VGM24_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154856.1"
                     /translation="MEENISDLPVYTDVEAVFKQRKTTEDRVQMMATCTVNGKPGRSV
                     WIEVPTVLYNGYKGKVRIIEFRIRSGMLDVLKVQKI"
     gene            complement(2653..2886)
                     /locus_tag="VGM24_05510"
     CDS             complement(2653..2886)
                     /locus_tag="VGM24_05510"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY4154857.1"
                     /translation="MWKESDKKLHKEFKFEDFSEAFAFMTRVALEAEKMNHHPRWTNE
                     YNKVEFWLSTHDAGDLVTEKDHKLAKKIDALIK"
     gene            complement(3006..>3459)
                     /locus_tag="VGM24_05515"
     CDS             complement(3006..>3459)
                     /locus_tag="VGM24_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154858.1"
                     /translation="GLLPLLGACTASYFISFSMMKSTIMTEKINRRGVITPDSYEPDV
                     IQSMHVSDVMGEDISNIQIKEPLRYYVYPETGMTLAIDLMDRYKTELMPVVSKENKKK
                     IIGVINPSLIFAAYRKYRYANEDQRSISLKRKGYEIMVRGRQRFRRDT"
BASE COUNT          932 a          822 c          790 g          915 t
ORIGIN      
        1 caatgtcttc cattttaaac aatacgtatt catttcccct gcgaaagacg attctgcgga
       61 aggtaatcct atggttgtcg ttattgaaaa tgcgaatccg gtttatatta ataaataaaa
      121 taaccggaat aaaaataaat actaaatgct ttcaaatggc aaaatgatct aaaatcccgc
      181 ttttcggggg agaaggggtt gaaagaccgg cgctgaatat ataaacgaaa aaacccccag
      241 gtttaatcag gggcttttca gagaacttga atttaaggtt ttcgctagga cggtaacaga
      301 tgaactcaag tggtttttcg ggatattcgg ttgtttcagc ggtattggat aagtcaaaac
      361 tagggcatcg atacatcctg gcaaagcgag gaatcactca attttgaatc gtagatttac
      421 gaatctttcc cagtttttat taaaatgaaa aaattactat ttattaccgt ccttttctgc
      481 ctggcagccg gatgcggccg gaaaaacagc ctccatcttt tccctggcgg caatcccctg
      541 attcaataca tcgggagaat agatttttca accgccgcca gaccccgctt ttggaatccc
      601 ggaacctata tcagcatccg gttcagggga agttcctgtg atgtcctggt caatgatgag
      661 gtgtcgggag gggaaaatca taattatctc gaaattattc tggataacca aaaaccggaa
      721 agaataaaac tgacggacag ggaaaacaat atccatatcg gcgatcacct ggcagcaggc
      781 gatcattcac tggtgattgt taaaaacacc gaagccggca tcggctggct ggaatttgcc
      841 ggtatccgtt gcgaaaaact gctgccggta aaactgccat ccaggaaaat ggaatgcatt
      901 ggcaattcaa ttacctgcgg aaccggcagc gatacaagcg tttttgcctg cgggcaggga
      961 caatggtatg accagcacaa tgcctggctg agctatggtc cgctcaccgc ccgcgcgctt
     1021 gacgcacagt atcatctaac agccgtctcc ggcatcgggc ttattcacag ttgttgccat
     1081 atggacgtac tgatgcccca ggtatttgac aagatcgata cccacgggaa taagataaaa
     1141 tgggacttta atgaatatca gcccgatgtg gtgaccattt gcctggggca aaacgatggt
     1201 gctggcgatt cagtgaattt cttcaatgcc tatacgggat ttattgcccg catccggaaa
     1261 gtttatccgc gcacgcagat cgttgcactg accagcccca tggcggatga attcctgaaa
     1321 aacgcaatgg tcagatacct gaccacgatc gtcgaatatg aaaaccggca gcacgacccc
     1381 aatatacatt tatatgtatt cagcaaaaga taccaccagg gatgcggtga ccatcccctg
     1441 ctttcggaac atcgggaaat cgcagccgaa ctcactgcct atattcaaaa actgatgggc
     1501 tggtaaggaa cggaccggtt ccggatggtt gaacagtctc ccctacttga tatgcgcccg
     1561 cagcatccag gcagtctttt cgtgtttctc catcagaccg gtgatatagt cgctggttcc
     1621 cgcatcctta tgggcatccg caaattcatc cacatttcca cggataaatt ctatgatgct
     1681 ttcatggtct tccagcaggt ttttgatcag gctcaggctg gtattcccgc cctttaattt
     1741 ttcagtcaga tgggtaaggt ccaaaaaatc cctgagcgtt gccggcgcgt aatgccctaa
     1801 ctgccggatg cgctctgcca cactgtccac cgtctttgcc gcttcttcat acaattcttc
     1861 gaaatataaa tgcacggaat gaaagtccgg tccctcgata ttccagtgcg cattcaatgt
     1921 tttggtatac aatacgaatt catctgccag caacttggcc agttgttcag ctaccgtttt
     1981 ccggttagca tccgaaatcc caatacttgt tttcatttta gtttatttag tggccgtgtc
     2041 cggggaccag cgccctgcaa accggatccg gaaccgttcc tgcgaaatta tcacatttcc
     2101 cggtaccttc gcgtattgtc ggctatggag gaatcattta ccataacggt tttggataag
     2161 gggatcgaaa aagagctggc ggggagattg cggatcagca cctataccta tcagtttctt
     2221 tttgagatcg gggattcgga gatcattctg gagcgggatg atgaaggaaa tttccgggcg
     2281 ctcggcagcc aggcggttcc aggaaaaaaa ccggatcctt cactggttca gaccctgatc
     2341 agggcaatgg aagaaataat gaaagaataa caaaatctct tcgacaatca gctatcatgg
     2401 aagaaaatat ttcggattta cccgtatata ccgacgtgga agcggtattt aaacaaagaa
     2461 aaaccacaga ggaccgggtt cagatgatgg ccacctgtac ggtcaatggc aagcccggcc
     2521 ggagcgtatg gatcgaagtt cccacagtcc tatataatgg ttataaagga aaggtccgaa
     2581 tcattgaatt caggatccgc agcggaatgc tggacgtgct gaaagtccag aaaatttaga
     2641 agctcatccc ggctatttta tcagggcatc aatctttttc gccagcttat gatctttctc
     2701 cgtaaccagg tctccggcat cgtgggtaga taaccaaaac tccactttat tgtattcgtt
     2761 ggtccaacgg ggatgatggt tcattttttc cgcttccagg gccacccggg tcataaaggc
     2821 aaaagcctcg gaaaaatctt caaacttaaa ttccttgtgc agtttcttat ccgattcctt
     2881 ccacataaga gattaattta gatttttaat gagtcccggt ccgttttcac aaccccattc
     2941 cgtaaccgca aaaagcttgc cctgatcagc ggccctgctt gggtattcgt acctgccttt
     3001 tcgcatcagg tatccctcct gaaccgctgc cttccccgga ccattatttc gtagcctttc
     3061 cgtttgaggg agatgctacg ctggtcttca ttcgcatacc ggtacttccg gtaagctgca
     3121 aaaatcaggg aagggttgat taccccaata attttcttct tattttcttt cgatacgacc
     3181 ggcattaatt ccgttttata gcggtccatt aaatcaatgg ccagggtcat gccggtctcg
     3241 ggatatacat aataacgcaa aggctctttt atctggatat ttgaaatatc ctcccccatg
     3301 acatcggaaa catgcatgct ttgaataaca tccggctcat agctgtcggg ggtgatgacc
     3361 ccgcggcggt ttatcttttc ggtcatgatc gtacttttca tcatagagaa agagatgaaa
     3421 taagaggccg tgcaggcacc cagcaaaggc aataaaccg
//
LOCUS       DASYCU010000275         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2525186, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000275 DASYCU010000000
VERSION     DASYCU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2525186"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="VGM24_05520"
     CDS             <1..1836
                     /locus_tag="VGM24_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4154859.1"
                     /translation="LSKIEAGRMNLEYGFLDVRQMTGVIKSLFEPLAKEKKLDFKITI
                     GPAVPEEIETDRMRVEQVLKNLLSNAFKFTTEGSVELQVKPDPGNDPRFIVFEVKDTG
                     IGIPDSKQALIFEAFQQADGSTKRKFGGTGLGLSISRELSKLLNGDLSVSSEENKGSV
                     FSFRLPVSRLFFPAKEEKHPAGSFEEMNPPATAPVSPEPPLTVTDIPKDIPDDREFIL
                     PGDKVILIVEDDTHFARALLDFTRDQGYKGIVAVRGDVGIQLAKTFNPRGILLDIQLP
                     VQNGWEVMEELKRDPKTRHIPVHIMSSFEARKESLQKGAVDFTSKPVAFEKMQEIFKK
                     IELTLKGEAPSKVLIVEENAKHARALSYYLGTYGIQPKIAESVEETIKIFHENKVDCV
                     ILAMGVSDMSGYEKLEAVKAAPDLGNIPIILFTGKSFSRSEEKRIRQYADSIVIKTAH
                     SYERMLDEVSIFLHIVDQKDRPRESPPMEKLGALDEVLKDNTALIADDDVRNIFSLTK
                     VLEQHKMKVIPAIDGKEALEMLELNPQVNIILMDMMMPEMDGYESIRKIRKIPAFRNL
                     PIIAVTAKAMVGDREKCISAGASDYISKPIDIDQLISLLRIWLYE"
     gene            1852..2667
                     /locus_tag="VGM24_05525"
     CDS             1852..2667
                     /locus_tag="VGM24_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY4154860.1"
                     /translation="MMVQDNEVRILLNDLIDRYGYDFTGYAGASLKRRINRLFEMDRF
                     PSFAEFRYRILNDAEYFLRLVEELTVNVTEMLRDPDFYKILREDVIPILATYPFIRIW
                     HAGCSTGEEVYSMAIMLSEAGLLHKSRLYATDINPKVLDKAKKGMFPASQMRKYSENY
                     IQSGGSRDFSSYYTSNYNLAKFDTALSDKMIFASHNLVSDSSFNEFQLILCRNTLIYF
                     DRDLQSRVLELFDSSLEVLGFLALGSKESIKFSPIAPKFKPAVSGHKIWRKIR"
     gene            2649..>2903
                     /locus_tag="VGM24_05530"
     CDS             2649..>2903
                     /locus_tag="VGM24_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY4154861.1"
                     /translation="MAQDQINRTELVLIGGSSGSLAVVLRILTALPAGFPIPILLLLH
                     RGNTQESLLGEVLRLKSGMTLVEVEEKEPIRPATVYFAPAD"
BASE COUNT          782 a          688 c          753 g          680 t
ORIGIN      
        1 ctttcgaaaa ttgaagccgg ccggatgaat ctcgaatacg gcttccttga tgtgagacag
       61 atgaccggcg tcatcaaatc cctttttgag ccgctcgcaa aagaaaagaa gctggatttt
      121 aaaataacca tcggccctgc ggttccggag gagattgaaa ccgacagaat gcgggtggaa
      181 caggtgctga aaaacctgct ttccaacgca tttaagttta cgacggaagg cagtgttgaa
      241 ctccaggtga agccggaccc cggcaatgat ccccgtttta tcgtgttcga agtgaaggat
      301 accggcatcg gtattccgga cagcaaacag gcattgatct tcgaggcctt tcaacaggcg
      361 gacggctcta ccaaaagaaa attcggaggc accggcctcg ggctttccat cagcagggaa
      421 ctcagtaaat tactcaacgg ggatctctct gtgagcagcg aggaaaacaa aggcagcgta
      481 ttcagtttcc ggcttcccgt ttcccggcta ttcttcccgg ctaaagaaga gaaacatccg
      541 gcaggctctt ttgaagaaat gaacccgccc gctaccgccc cggtttctcc cgaacctccg
      601 ctaacggtga cggacattcc aaaagacatt ccggacgaca gggaattcat tctacccggg
      661 gataaggtga ttttgatcgt ggaggacgat acgcattttg ccagggccct gctggatttt
      721 acgcgggacc agggttacaa ggggatcgtc gccgtgcgcg gggacgtggg tatccagctg
      781 gccaaaacat tcaacccgag aggcatttta ctggatatcc aattgcccgt acagaatggc
      841 tgggaagtga tggaagagct gaaacgcgat ccaaaaacca ggcatatccc ggtacatatc
      901 atgtcttcct ttgaggcaag aaaagaaagt cttcagaaag gagcggtgga tttcaccagt
      961 aagccggtgg cttttgaaaa aatgcaggag atattcaaaa agatcgaatt gaccctgaaa
     1021 ggagaagctc cttccaaagt gcttattgtg gaagaaaacg caaaacatgc ccgggcgctc
     1081 agctactatc tgggaacata tggcattcag ccaaaaattg cggaatccgt ggaagagacc
     1141 ataaaaatat tccacgagaa taaggtggat tgcgtaattc tggctatggg cgtttccgat
     1201 atgagcggat atgaaaaact ggaagccgtt aaagctgcgc cggatctcgg gaatattccg
     1261 atcatcctct tcaccgggaa gagcttttcg aggtctgaag aaaaacgaat ccggcaatat
     1321 gccgattcca tcgtgatcaa aacggctcat tcttacgaaa ggatgctgga tgaggtttct
     1381 attttcctgc atatcgtgga tcagaaggac cggccgcgcg aatctccccc gatggagaaa
     1441 ctcggtgccc tggatgaagt attaaaagac aataccgccc tgattgccga tgatgatgtg
     1501 cggaatattt tttcgctgac caaggtgctg gagcagcaca agatgaaagt gataccggct
     1561 attgacggaa aagaggccct ggaaatgctg gagctgaatc cgcaggtcaa tatcattctg
     1621 atggatatga tgatgccgga aatggatggc tatgaaagca tcaggaaaat acggaaaata
     1681 cctgctttcc ggaatctgcc catcattgcc gtaacggcca aggccatggt gggcgaccgg
     1741 gagaaatgca tcagcgcagg ggcttcggat tatatttcga aacccattga tatcgatcag
     1801 ctgatttcgc tcctgcgcat ctggctgtat gaataaccga atttatagaa gatgatggta
     1861 caggacaatg aggtgaggat attattaaat gatctcatag atcgttacgg ttatgatttt
     1921 acgggttatg ccggggcctc cctgaaaaga aggattaacc ggctgtttga aatggaccgg
     1981 tttcccagct ttgcggaatt ccggtacaga attttaaacg atgcggaata tttcctgcgg
     2041 ctggtggaag aactgaccgt taatgtgacc gagatgctgc gggatccgga tttttacaaa
     2101 atactccggg aagacgtgat cccgatactg gccacctatc cgtttatccg gatctggcat
     2161 gccggctgct ccacgggaga agaggtctac tcgatggcca tcatgctttc cgaggccggg
     2221 ctgcttcata agtcccgctt atacgcgacc gacatcaatc cgaaagtgct cgacaaggca
     2281 aaaaaaggga tgttcccggc cagccagatg cggaaatatt ccgagaacta tatccaatcg
     2341 gggggaagca gggatttttc ttcctactat acctccaatt ataacctggc aaaattcgat
     2401 accgcgctaa gcgataaaat gatctttgcg tcgcacaacc tggtttccga ttcatcgttc
     2461 aatgagtttc agctgatcct gtgcaggaat accctgatct atttcgacag agacctgcaa
     2521 tccagggtgc tcgagttgtt tgacagcagc ctggaagttt taggttttct tgcgctggga
     2581 agcaaagagt ccatcaagtt ttctcccatt gcccctaaat ttaaacctgc cgtttccggc
     2641 cataaaatat ggcgcaagat cagataaatc gaacagaact ggtgcttatc ggaggttcat
     2701 ccggaagtct tgccgttgtc ctccgcattc tgacggccct tcccgcaggt ttccccatcc
     2761 ccatcctcct gctgttgcac cggggcaata cacaggaatc gctgctcggt gaagtgcttc
     2821 gccttaaatc cggtatgacc ctggtggaag tggaagaaaa agagccaatc cggccggcca
     2881 ccgtttactt tgctccggca gat
//
LOCUS       DASYCU010000276         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4266215, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000276 DASYCU010000000
VERSION     DASYCU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4266215"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VGM24_05535"
     CDS             <1..1262
                     /locus_tag="VGM24_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY4154862.1"
                     /translation="FICIHAIGGVEICDDRQMEFKYPGRPAEKIELKSAAVPALLWEY
                     MQKFEVEPATEKPVKTAQSLFGYTTFDAVRFFDTINLPDTKEAPLIPLMRYRLYQYVI
                     AINHFKDELYICENRVPDMESEVEVVESRIRSKDVPVYPFRVNGAEKSNLTDPVYMEI
                     VEKGIKSCLRGDVFQIVLSRRYQLPFIGDEFNVYRALRNVNPSPYLFYFDYGDYRLMG
                     SSPESQVLIKDGKAVMHPIAGTCRRSGNDEVDKEMADVLLKDAKENAEHVMLVDLARN
                     DLSRKCDEVSVTHFREIQYYSHVIHLVSEVKGKLARESNPFELLAATFPAGTLSGAPK
                     IRAMELIAGYEPTARSYYGGCIGFIGFNGDCTQAIMIRSLMSRRNTLTYQAGAGVVAA
                     SKPENELEEVNHKLGALKRAIELAQTI"
     gene            1275..1865
                     /locus_tag="VGM24_05540"
     CDS             1275..1865
                     /locus_tag="VGM24_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY4154863.1"
                     /translation="MKILVFDNYDSFTYNLVHLVEKIVGGKVDVYRNDRITLEKAGQY
                     DKIILSPGPGLPSGAGLLLPLIKELAATKSILGVCLGHQAIAEVFGGRLLNLSAVFHG
                     VSLPVDIVKRKEAGESANADLFRGLPDQFLAGRYHSWVVDPVGFPDELEITATDAGGH
                     IMALRHRKFDVLGVQFHPESVLTPQGEKIMENWLEN"
     gene            1887..>2507
                     /gene="trpD"
                     /locus_tag="VGM24_05545"
     CDS             1887..>2507
                     /gene="trpD"
                     /locus_tag="VGM24_05545"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY4154864.1"
                     /translation="MKKILQILFEHKTLSREQAKDVLVHISRGMYNESEITAFVTVFL
                     MRSITMEELEGFRDALLELAVKVDLDGLEVIDIVGTGGDGKNTFNISTLSCFIVAGAG
                     RKVAKHGNYGATSISGASNVMEQLGYRFSNDSGKLKKEVEQTNICFLHAPLFHPALKA
                     VGMIRKNLGIRTFFNMLGPMVNPASPKFQLVGVYSLEMARIYTYLLQ"
BASE COUNT          626 a          601 c          712 g          568 t
ORIGIN      
        1 cattcatctg catccatgcc atcgggggcg tggagatttg cgacgaccgc cagatggaat
       61 tcaaatatcc cgggaggcct gctgaaaaaa ttgaattgaa atctgccgcg gttccggctt
      121 tgctctggga atatatgcag aaatttgaag tggagccggc caccgagaag ccggtaaaaa
      181 cggcccagag cctttttggg tatacgacct tcgatgcggt ccgttttttc gataccatca
      241 acctgccgga taccaaagaa gcccctctga ttcccctgat gcgctaccgg ctttatcagt
      301 atgttatcgc catcaatcat tttaaggatg aattgtatat ctgtgagaac cgggtaccgg
      361 atatggagag tgaagtggag gtggtggagt cgaggatccg ctccaaagac gtacccgtat
      421 atccatttcg cgtaaacggc gcagaaaaaa gcaatctgac agatccggtg tatatggaga
      481 tcgtcgaaaa agggataaaa agctgcctgc ggggagatgt attccagatc gtgctgagcc
      541 gccgttatca actgcctttc attggggatg aattcaatgt ataccgtgcc cttcgcaatg
      601 tgaatccgag cccctatctg ttttatttcg attacggcga ttaccgcctc atgggatctt
      661 ccccggaatc ccaggtgctc attaaggacg ggaaagccgt gatgcacccg attgccggaa
      721 cctgccgccg gtcaggaaat gacgaagtgg ataaggaaat ggcggacgtc ctgctgaagg
      781 acgccaagga gaatgccgaa catgtgatgc tggtggacct cgcccggaac gacctgagcc
      841 ggaaatgcga tgaggtttcg gttacccatt tccgggagat ccagtattat tctcacgtga
      901 tccacctggt gagcgaggta aagggaaagc tggccaggga aagcaatccc tttgaattgc
      961 tggccgccac ttttcccgcg ggcactctga gcggcgctcc gaagatccgg gctatggaat
     1021 tgattgccgg gtatgagccc acggccagga gttattacgg cggctgtatc ggattcatcg
     1081 gattcaacgg ggattgcacc caggccatca tgatccgaag cctgatgagc cgtcgcaata
     1141 ccctcaccta tcaggccggc gccggcgtgg tggccgccag caaacctgaa aatgaactgg
     1201 aggaagtaaa ccataagctg ggcgcattaa aacgggcgat tgagctggcc caaaccattt
     1261 aagcaggaag cattatgaaa atactggtat ttgacaatta cgattcgttc acgtacaacc
     1321 tggtacatct ggttgaaaaa attgtcggcg gcaaagtgga tgtttaccgg aacgaccgga
     1381 taaccctgga aaaagcaggt cagtatgata agatcatttt atctcccggt ccgggtcttc
     1441 cgtccggggc cggattgctc cttcccctga ttaaagagct ggctgccacc aaatccatac
     1501 tgggggtttg cctgggccac caggccatag cagaggtgtt cggcggcagg ttgctgaacc
     1561 tgtcagcggt atttcacgga gtatccctcc cggtggatat cgtcaaacgg aaggaagccg
     1621 gagaatccgc gaatgccgat ctgttccggg gtctgccgga tcagttcctg gcaggccgct
     1681 atcacagctg ggtggtggat ccggttggat ttccggatga actggagatc accgcaaccg
     1741 atgcgggagg acatatcatg gccctccggc accggaaatt cgatgtgctc ggcgtgcagt
     1801 ttcatcccga aagcgtgctg accccgcagg gggagaaaat tatggagaac tggctggaaa
     1861 attaagttcc cgccttaact ggaattatga aaaagatatt gcagatttta tttgagcaca
     1921 aaaccctttc ccgggagcag gcaaaggatg ttctcgtcca tatttcccgg ggaatgtaca
     1981 atgaatccga gatcaccgca tttgtaacgg tattccttat gcgaagcatc acgatggagg
     2041 aattggaagg attccgggat gcgctgctgg aactggcggt gaaagtggat ctggacgggc
     2101 ttgaggtgat cgatatcgtg ggaaccgggg gagacgggaa aaacacattc aatatttcaa
     2161 ccctgtcctg ttttattgtt gccggggccg gaagaaaggt ggccaaacac ggaaactacg
     2221 gagccaccag catcagcggg gcctccaatg tgatggaaca gctgggctac cggtttagta
     2281 acgatagcgg gaaactgaaa aaggaagtgg agcagaccaa tatttgtttt ttgcatgccc
     2341 cgctctttca tccggcatta aaagcggtgg ggatgatccg gaaaaacctc ggtatccgga
     2401 ctttctttaa catgctgggt ccgatggtaa atccggcttc ccccaaattt cagctggtgg
     2461 gggtatatag cctggaaatg gcccggatct atacctatct cctgcag
//
LOCUS       DASYCU010000277         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_785576, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000277 DASYCU010000000
VERSION     DASYCU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785576"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VGM24_05550"
     CDS             complement(<1..800)
                     /locus_tag="VGM24_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY4154865.1"
                     /translation="MSNELAIHGHGAMVSHDEHHDHASHEHKQNFITKYVFSQDHKTI
                     CKQFLITGIIWAIIGGLMSVIFRLQLGYPDSTFPWLEDILGKWAKGGKLTPEAYYALV
                     TMHGTILVFFVLTGGLSGTFANFLIPLQIGARDMASPMLNMLSYWFFWLASVVMLCSL
                     FIETGPASGGWTIYPPLSALGDANLGSQAGMDFWIVGMACFVISSLLGGLNYITTILN
                     MRTKGMSMTRLPLTIWALLFTAILGVLSFPVLLSGLILLLFDRHFGTS"
     gene            complement(835..>1758)
                     /locus_tag="VGM24_05555"
     CDS             complement(835..>1758)
                     /locus_tag="VGM24_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY4154866.1"
                     /translation="VFLVLGLIGVYWCNELYKGKILGEAASDHGVKIDRMLYITFAIT
                     GVVFFITQILLFWFSFKYQHSEKRKAYFFPHNNKLEMIWTVIPAIALTVLVGFGLVYW
                     FQITGDPPKNAMEIEVTGKQFNWIFRYPGKDNIFGKKYYRNISDKENNTLGLLWDDEA
                     THDDIVVFNTMYLIVNQPVKLIINSRDVIHDVGLAEFRMKMDAVPGTPTTMWFTPLYT
                     TAEMKKITGNPDFEYEISCDQMCGRGHFSMRGEIQVVTQEEYILWRAKQQSNYAQVMK
                     DKAPATTKDSTAAPAAKLKLNNPGGLVAQTR"
BASE COUNT          450 a          453 c          426 g          429 t
ORIGIN      
        1 aaactggtcc cgaaatgccg gtcgaacagc aggagtatca ggccggagag caacaccgga
       61 aacgacaaca cgccgaggat agccgtaaac agcagggccc agatggtcag cggcaaacgg
      121 gtcatgctca tacccttggt ccgcatgttc agaatggtcg tgatataatt caaaccgccg
      181 agcagggacg aaattacaaa acaggccatg ccgacaatcc agaaatccat tccggcctgc
      241 gaacccaggt tcgcgtcgcc cagcgcactg agcggcggat aaatcgtcca gcctccactg
      301 gcgggtcctg tttcgataaa cagggaacag agcatcacga cgctggcgag ccagaaaaac
      361 caatacgaaa gcatattgag catcggcgaa gccatgtcgc gggcgccgat ctggagtggt
      421 atcagaaaat tggcaaaagt tccgctcaaa ccacccgtca gtacaaagaa tacaaggatg
      481 gttccatgca tcgtcaccag cgcatagtaa gcctccggag tgagctttcc gcccttagcc
      541 catttgccca ggatgtcttc cagccagggg aacgtggaat cgggataacc cagctgcagc
      601 cggaaaatga ccgacatcaa tccaccgatg atggcccaga taatccccgt aatcaaaaac
      661 tgtttgcaaa tggttttgtg atcctggctg aagacgtatt tcgtaatgaa attctgtttg
      721 tgttcgtgcg aagcatggtc atgatgttca tcatgagaaa ccattgctcc gtgtccgtgt
      781 atcgccaatt cgttactcat cacaaaaaat ttaatttctg tttgtataat ctttttacct
      841 ggtctgcgcc accaatccgc ccggattgtt caacttcaat ttggcagcgg gtgcagccgt
      901 agagtctttg gtggtcgcgg gggccttgtc tttcatcacc tgggcatagt tggattgctg
      961 tttggccctc cacaatatgt attcctcctg ggtaaccacc tgaatttctc cgcgcatcga
     1021 aaaatgtcct ctgccgcaca tctgatcgca ggagatctcg tattcaaaat ccgggttacc
     1081 ggttatcttt ttcatttccg ccgttgtgta caaaggggta aaccacatgg tggtgggtgt
     1141 tcccggaacg gcatccattt tcatcctgaa ttcggccaga cccacatcgt ggatcacatc
     1201 cctcgaattg atgatcagct taacgggctg gttgacgatc aggtacatgg tgttgaaaac
     1261 cacaatgtca tcatgcgtgg cttcatcatc ccaaagcagt ccgagggtgt tgttttcctt
     1321 atcgctgatg ttgcggtaat atttttttcc gaaaatatta tccttacccg ggtaccggaa
     1381 gatccagttg aattgttttc ccgtcacttc gatttccatg gcgttttttg gcggatcgcc
     1441 ggttatctga aaccaatata ccagaccaaa ccccaccagc accgtgagcg cgatggccgg
     1501 aataacggtc cagatcattt ccagtttgtt gttgtgcggg aagaagtaag cctttctttt
     1561 ttcagaatgc tggtacttaa aggagaacca gaacaggagg atttgcgtga tgaaaaaaac
     1621 gacgccggta atggcaaacg tgatgtagag catcctgtca atcttcaccc catggtccga
     1681 tgccgcctcg ccgagaattt ttcccttgta caattcattg caccagtaaa caccgatcag
     1741 gccgagcacc aggaaaac
//
LOCUS       DASYCU010000278         4220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_207423, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000278 DASYCU010000000
VERSION     DASYCU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207423"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VGM24_05560"
     CDS             complement(<1..1122)
                     /locus_tag="VGM24_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY4154867.1"
                     /translation="MKYFLGFDIGSSSVKAALLEADSGKPLAAAFSPETEMAIQAPEP
                     GFAEQDPELWWEELIRATRLLRKKHAFSKDEIGGIGISYQMHGLVCIGKDHKPLRPAI
                     IWCDSRAVAYGNQAFDQLGHSFCLERFLNSPGNFTASKLKWVKENEPGVYKKIFRILL
                     PGDFIALKLTGEAVTTVSGLSEGIFWDYRENNIAKTLLDFYGIDSGLLAPVVPTFGDQ
                     GKLNAEAAALLDIAAGTPVCYRAGDQPNNAFSLNVLEPGEIAATAGTSGVVYGVTGQA
                     AHDPRSRVNPFVHVNHRPERPRYGILMCLNGTGILNSWLRKNFFGSSSYDEMNVEAEK
                     AEPGAGGIFCYPFGNGAERILENRNPGAVIQGIDFNRHSR"
     gene            1209..1562
                     /locus_tag="VGM24_05565"
     CDS             1209..1562
                     /locus_tag="VGM24_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154868.1"
                     /translation="MLESKDQPLASIGVYRRRIRKSLFTGMAILLACLLIGLLGYHYT
                     AGIPWLDALHNASMILSGMGPVVEIKTTSGKWFSSFYALFSGVIFITNIGIILAPAIH
                     RFFHRMHVQTEKGKS"
     gene            complement(1565..3589)
                     /locus_tag="VGM24_05570"
     CDS             complement(1565..3589)
                     /locus_tag="VGM24_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3858 domain-containing protein"
                     /protein_id="HEY4154869.1"
                     /translation="MKSKKWICLFLCACFFQHKGNAQDKLDIKFGKISPADFDLSGEK
                     FDSGANAVIIADIGNTSFEGNSRGDFTLIYTRLVRVKILNKNGFDIADNRIMLYHNST
                     GDAEKLSNLKASTFNLENGVVTETKLDEKSVFDEKYSRNIDIKKFTLPALREGSVYDL
                     TYTIKSDFYTHLRNWKFQGDYPRLWSEYQVTIPTMFHYVEHTKGDGHFDVNTVKSVFQ
                     SFTIRVSNGTSSDDVYHLSGNSIQQRWVKKNVPALKDEPFTSSIDNYMAEVSFQLHYI
                     QWTETSERHDYLSTWFIASDKLLQDDDFGGALDKDNHWMSEELKGITADCKSDEEKIK
                     KIYCFVRDNFSCTDHDGIYARSTLKDVYKKRSGNVAEINLLLTAMLRHENITADPAIL
                     STRENGIADQSYPLMDQFNYVICVAYSNGKMLKLDASQPYNGFGKMVMDCYNGQARII
                     NKEKPYLINLSPDSIQESKLTTVFIMNDEKGNPSGSFQTTLGNEESYQVREEIRKTSE
                     KDYFKKIQTGYGSDLSLENPVIDSLGSLSNPVSVHYLFDLKNLLSADVIYFNPMMSEM
                     YKNNPFKPLDRHYPVEMPYKMDETYVLNMDIPKGYQVDELPKSARVAYNETDGLFEYL
                     IQKGENDIQMRVHLKLNKAFFPTEEYSTLRDFFGYVVKKENEQIVFKKIH"
     gene            complement(3640..>4220)
                     /locus_tag="VGM24_05575"
     CDS             complement(3640..>4220)
                     /locus_tag="VGM24_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154870.1"
                     /translation="VQSQTSYKAACQDQLEEFIHRLSKEDQLKSLKTQFDLPTYDVNS
                     FRYQEDFSHRLPVIQESLQLTVTDYAQVSGKRIFINPDILTRSRIKLEEDKDRQLGVE
                     LSSESCYIDSVQIAVPAGYQTESLPENIHLQGKFGNYETHIAIEPDKIVYYRKWERYS
                     GRFPVSEYNDLVKFYNEIYKSDHSKIVLVKKS"
BASE COUNT         1037 a         1059 c          928 g         1196 t
ORIGIN      
        1 tctgctgtgg cggttgaaat cgatcccctg aataaccgct cccgggtttc tgttttccag
       61 tatccgctcg gctccgttgc cgaacgggta acaaaaaata ccacctgccc cgggctcggc
      121 tttttccgct tccacattca tttcatcata agacgaggat ccaaagaagt tttttctcag
      181 ccagctgttc aggataccgg tgccgttcag acacatcagg atcccgtacc gcggtctttc
      241 cggccggtga tttacgtgaa caaacggatt tacccgggat cggggatcgt gtgcggcctg
      301 cccggtaact ccataaacca ccccggaagt tccggcggtg gctgcgattt cgccaggctc
      361 cagcacattt agtgaaaatg cattattggg ctgatcacca gcccggtagc aaaccggcgt
      421 gcctgccgca atatccagta atgctgccgc ttctgcattc agctttccct ggtctccgaa
      481 tgtgggaacc acaggcgcca gcagtccgga gtcaattcca taaaaatcca ataaggtttt
      541 tgctatattg ttttcccggt aatcccagaa aataccttcg gacaatccgg agaccgtggt
      601 gaccgcttcc ccggtcagct tcaacgcgat aaaatctccg ggcaggagta tgcggaatat
      661 ttttttatag acccccggtt cattttcctt tacccatttc aatttggacg cagtgaaatt
      721 tccgggagaa ttcaggaagc gttccaggca aaaggagtgg cccaactgat caaaagcctg
      781 gttaccatag gctacggcac gactgtcgca ccagataatg gccgggcgca atggtttatg
      841 atctttgcca atgcagacca atccgtgcat ctgataggaa atgccgatcc cgccgatctc
      901 gtctttggaa aaggcgtgtt ttttgcgaag cagccgggtc gcccggatca gctcctccca
      961 ccaaagctca ggatcctgtt ccgcaaatcc gggttcggga gcctgaatag ccatttcggt
     1021 ttccggagag aaggctgcag ccagcggctt cccgctatct gcttccagta atgctgcttt
     1081 taccgatgaa gaaccaatat caaaccccag gaaatatttc acaggcaaaa aaatgtgggc
     1141 tgtaagataa ttattattac ccactccgtc ctaatgtaac ttgttacatt tgagaacaat
     1201 ccattaaaat gctggaatca aaagaccaac ccctcgcgtc cattggggtg tatcgcaggc
     1261 gcataagaaa aagccttttt accggcatgg caatcctgct ggcctgcctc ctcatcggcc
     1321 tgttgggtta tcattatacc gccggtattc cctggctgga tgcattacac aatgcctcca
     1381 tgatcctgag cgggatgggt cccgtggtgg aaattaaaac gacctccgga aaatggtttt
     1441 cttcttttta tgcgctcttc agcggcgtca ttttcattac caatatcggg atcatactcg
     1501 cccccgctat tcaccgcttc tttcacagaa tgcatgtgca gaccgagaag ggaaaatcct
     1561 gatctcagtg gatttttttg aatacgatct gttcattttc tttctttacc acgtatccga
     1621 aaaaatcccg cagggtggaa tattcctccg tagggaaaaa tgctttattg agttttaaat
     1681 gtacccgcat ctgaatatca ttttcacctt tttgaatcag gtattcaaac agtccgtccg
     1741 tttcattata agccaccctc gccgatttcg gcagttcatc cacctggtaa cccttgggga
     1801 tatccatgtt cagtacatat gtttcatcca ttttataagg catttcaacg ggataatgcc
     1861 ggtccagggg tttgaacggg ttgttcttat acatttcaga catcatggga ttaaaataaa
     1921 tgacatccgc ggagagcagg ttcttaagat caaaaagata atggaccgat accgggttac
     1981 tcaggctgcc caaagaatca ataaccgggt tctccagact cagatcgctt ccgtaaccgg
     2041 tttgtatctt tttaaagtaa tccttctcgg aggttttcct gatctcttcc cggacctgat
     2101 aggattcctc gttgccgagg gtcgtctgaa aactaccgga cggattcccc ttttcatcat
     2161 tcattatgaa cacggtggtc aatttggact cctggatcga atccggcgaa aggttgatga
     2221 gataaggttt ttccttgttg atgatcctgg cctggccatt gtagcaatcc atgaccattt
     2281 ttccaaaacc attgtaaggc tggctggcat ccagtttaag cattttaccg ttggaatagg
     2341 caacgcaaat aacgtagttg aactgatcca tcagcggata ggactggtcc gcaataccgt
     2401 tctcccgggt actcaggatg gccggatcgg cggtgatatt ctcatgccgc agcatggcag
     2461 tcaataacag attaatttca gccacattcc ctgaccgctt cttatacaca tccttcaggg
     2521 tggagcgggc atagatcccg tcatggtccg tgcaggaaaa attatcccgg acaaagcagt
     2581 aaatcttctt tatcttttct tcatccgatt tacagtccgc ggtaattcct ttcagttcct
     2641 ccgacatcca atggttatcc ttgtcaagcg ccccgccaaa atcatcatcc tgtaataatt
     2701 tatcgctggc aatgaaccag gtgcttaagt aatcgtgccg ctcggaagtc tccgtccatt
     2761 gtatatagtg aagctggaaa gaaacttctg ccatataatt gtcgatggaa ctggtaaatg
     2821 gctcgtcctt taaggccggc acattcttct tgacccatct ttgctggatg gaattgccgg
     2881 ataaatggta aacatcatcg ctggaagtcc cgttgctgac ccgtatggta aagctctgaa
     2941 aaacgctttt aacggtattg acatcaaaat ggccatctcc cttggtatgt tccacgtagt
     3001 gaaacatcgt tggaatggta acctggtatt cgctccataa ccggggataa tctccctgaa
     3061 atttccagtt ccttaagtgg gtatagaaat ccgattttat cgtataggta agatcataga
     3121 ccgacccctc ccgcaaggcg ggcagggtaa atttcttaat gtcaatgttc ctgctgtact
     3181 tctcgtcaaa caccgatttt tcgtccagct tcgtctccgt caccaccccg ttctccaggt
     3241 taaaggtgga tgctttcagg ttggaaagct tttccgcatc tccggttgaa ttgtgataca
     3301 gcataatccg gttatcggct atatcaaaac cgttcttatt taatattttc acccgcacaa
     3361 gccgggtata gatcagtgta aaatctcctc ttgaatttcc ttcgaacgag gtatttccga
     3421 tatccgcaat gatcaccgcg ttggcaccgg aatcaaattt ttccccggaa agatcaaaat
     3481 ccgcggggct gattttgccg aactttatgt ccagcttatc ctgcgcattc cctttatgtt
     3541 gaaaaaaaca ggcacaaagg aacaggcaaa tccatttttt acttttcatg gttgtctagg
     3601 tttaaggggt attatcgggt taacgtccgc atccggtttt caactcttct tcaccagcac
     3661 aatttttgaa tggtccgatt tataaatctc attatagaac tttaccaggt cgttatattc
     3721 cgataccggg aaacggccgc tataccgttc ccattttcta tagtacacaa ttttatccgg
     3781 ttcaatggca atatgagttt cgtaattccc gaatttcccc tgcaaatgaa tattttcggg
     3841 tagcgattcg gtctggtatc cggccggaac tgcgatctga accgaatcga tataacagga
     3901 ttcgctgctt agctccacgc ccagctggcg gtccttatct tcttccagct tgatccggga
     3961 acgcgtcagg atatcgggat tgataaatat tctttttccg ctgacctgcg catagtcggt
     4021 tacggtaagt tgcagggatt cctgaattac gggtaaccgg tggctgaaat cctcctggta
     4081 acggaaagaa ttcacatcat atgttggaag atcaaactgg gtctttagcg acttcaactg
     4141 atcttctttt gacaaacggt gtatgaactc ctccagctga tcctgacagg ccgccttata
     4201 actggtttga ctctgaactt
//
LOCUS       DASYCU010000279         4877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2235482, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000279 DASYCU010000000
VERSION     DASYCU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2235482"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2704
                     /locus_tag="VGM24_05580"
     CDS             <1..2704
                     /locus_tag="VGM24_05580"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4154871.1"
                     /translation="AGGEALRAALLKVLEEDEAGVLKPRRLQRASDLLDEVIVERPPI
                     EVKEDTVEYNASAFKVKPNAYAEDILKKLPGVQVDKDGNVTAQGEQVQKVYVDGKEFF
                     GTDPKLATKNITADMIESVQVFDDMSDQAKFTKIDDGSRQKALNIKLKKDKRTGYFGR
                     ASMGYGSDNRYAGTLSFNRFREDQRISVIGGSNNVNQQNFNFSDIVTSMGGFGARNAG
                     GGGGGGRGGGGGYRGGNAGLSTSLGTGSSATGITQSTSGGINYNNKFGDKFQLAASYF
                     FSNSNRELDQSSFQQSFFPGDSASNQNSQSTSNNINQNHRFTARMEYTIDSSTSLLLI
                     PSLTLQHSQSSSEDSSSTEAIKGNNQYTAITGFTQNTNLRDGLSFNNNLLFRKRFKLP
                     GRTITIGINTSVNNSNGNGTNFAPLEFYAPNGTPDSLFMQNIHNTQKTSSQNNVLSLS
                     YTEPLGVGKLLEFNYAYTNNNNISDRRAMNYDSTSEKYDQVNLLQTNYFKNGFIANRF
                     GLNFRMAKEKFNLQIGSGVQFSSLSSRSLRAATGKDSTVNYSFINVIPTLFFNYKFSR
                     TKNLRISYRGHTNQPNITQLQNVPDVTNPLQIKTGNPDLKNEFENNMNIRYNSFNPST
                     YKFFSVNLVLDNTMNNIVNRIDSIGHGVQLITPINLNGTFSGNSFVTFGIPLRGRMKG
                     SNLNFNNSIAYARNPSMLYNQINVSTTWVVTQTAGINLVFNDKLNLGLNGSVSYNQAA
                     YSVQKNLNEEYFSQNYSADISYSFIKDWVFYTDYSQYIISGRAAGYNQTIPLWNASIA
                     REIFKKRNGEIKVSVNDLLDQNKSITRTVGDNYLTDTRSNVVKRYFMLSFLYNLNRSG
                     NGAKRNDGMPRMPRGMQREMQQLRSEPGGRPAPGAQAAPPPGRP"
     gene            3053..3625
                     /locus_tag="VGM24_05585"
     CDS             3053..3625
                     /locus_tag="VGM24_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154872.1"
                     /translation="MRILLLTSALLLNTALLFSQDDVFSNKTNQALEKVIRDYPNRFH
                     NIKGEMISQHANTAEYKSVVMVPGASSCTISKNNISSTELYSWSCTAFNSRDFLAARN
                     RFKEIYEQVENSIIKVDGQKPFILSGQYRTPTEMRDMNVIPFELLPAVGDMKNLKIEL
                     SLEKSLAFWKVKLNVYEGDPKLESATATVQ"
     gene            complement(3629..>4877)
                     /locus_tag="VGM24_05590"
     CDS             complement(3629..>4877)
                     /locus_tag="VGM24_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4154873.1"
                     /translation="VDHDIPSQLLGDPVRIRQIIVNLLGNAIKFTMEGEIYVSIRKEA
                     ELYWVDGKKYQNFVIQVKDTGIGIPKEKLQKIFESFTQADNSTTRKYGGTGLGLAISK
                     SLAELMHGTLSVESEAGSGSVFSLSLPLEVANDQPEILLKPKPSLKKVLVVDDNVTNL
                     HLMEETLNYFQIYCETCTNGPDALLKIEMANSSGAPFDLIITDHHMPGMDGINLVKEI
                     KHSIPGAIQPFILMLSSLEKNLYQNEADKTGINKFISKPVKLHELNSTLLSLFEKNMQ
                     NDSLHPSFESFEKITQSASILVVDDDPVNMMLISEVLKRMGFDIIQMQNGREVIEMLP
                     HYDPVLIFMDVNMPEMDGYTTTRIIRKMQTAQSRIPIIALTADAMKGDKERCLDAGMN
                     NYISKPFKLEEIEAVLREYLLLV"
BASE COUNT         1303 a         1230 c         1157 g         1187 t
ORIGIN      
        1 cgcgggcggc gaggccctgc gcgcggccct gctcaaggtg ctggaggagg acgaggccgg
       61 cgtgctcaag ccgcgccggc tgcagcgcgc gagcgacctg ctggacgaag tcatcgtgga
      121 aaggccgccc atcgaggtaa aagaagatac ggtcgaatac aatgccagtg cattcaaggt
      181 aaaacccaat gcatatgccg aggatatatt gaagaaactt cccggcgtgc aggtggataa
      241 ggatggaaat gttacggccc agggtgaaca ggtacaaaaa gtatacgtgg acggaaaaga
      301 gttttttgga acggacccca aactcgccac gaaaaatatt acggcagata tgattgaatc
      361 cgtccaggtt tttgacgaca tgagcgatca ggcaaaattt acaaagatag atgacggcag
      421 ccggcagaag gccctcaaca tcaaactgaa aaaggataag cgaacgggct atttcggaag
      481 ggcttccatg gggtatggct ccgataaccg gtatgccgga accctctcct ttaaccggtt
      541 ccgcgaagat cagcgcatct cggtgatcgg tggttcaaac aatgtcaacc agcagaattt
      601 caatttttcc gatatcgtta ccagcatggg cggctttggc gcccggaatg cgggcggcgg
      661 tggcggtggc ggcagaggag gcggtggcgg ctaccgggga ggaaatgccg ggctttccac
      721 ttccctggga accggatcca gtgcaaccgg cattacccaa tccacatcgg gcggtataaa
      781 ctacaataat aaattcggag ataaatttca gctggcggcc agttattttt tctccaacag
      841 taaccgggaa ctggatcaat ccagttttca gcaatccttt ttcccgggag attcggcttc
      901 caatcaaaac agtcaatcca cttccaataa tatcaatcag aatcaccggt tcaccgccag
      961 aatggaatat acgattgaca gctcgacctc cctcctgctg atcccatcac ttacgcttca
     1021 gcattcccaa tcctccagtg aggattcttc cagcacggaa gccatcaagg gaaataacca
     1081 gtacaccgcc attaccgggt ttacacagaa taccaatctg cgcgacgggc tgagctttaa
     1141 caataacctg ctattccgta aacggttcaa acttcccggc cggaccatta ccattgggat
     1201 caatacttcc gtcaataaca gcaacggtaa cggaaccaat tttgcacccc tggaatttta
     1261 tgcgccaaac ggcacgcccg acagcctgtt catgcagaat attcacaata cgcagaaaac
     1321 aagttcgcaa aataatgtgc tcagcctttc ctataccgaa cccctgggcg tagggaagct
     1381 gctggaattc aactatgctt ataccaacaa caacaatatt tcggaccgca gggcaatgaa
     1441 ttatgattcg acgagtgaaa aatatgatca ggtgaacctg ttgcagacca attattttaa
     1501 aaacggtttt attgccaacc gctttggtct gaacttcagg atggcaaaag aaaaattcaa
     1561 tctgcagatc gggagcggtg tgcaattctc gtccctgtcc tccaggagct tgcgggccgc
     1621 caccgggaaa gacagtacgg tgaactactc ctttatcaat gtgatcccca cgctgttttt
     1681 caattataag ttcagccgga caaagaacct gcgcatcagt taccggggtc ataccaacca
     1741 gcccaatatc acccagctgc aaaatgtacc ggacgtgacc aatcccctgc agatcaaaac
     1801 cggcaacccc gacctgaaaa atgagttcga aaacaatatg aatatccgct ataacagctt
     1861 caatccgtct acctacaaat tcttttccgt gaacctggta ttggacaata ccatgaacaa
     1921 tattgtcaac cggatcgatt ctatcgggca tggggttcaa ctcatcaccc cgataaatct
     1981 caatggaacg ttttccggaa actcttttgt cactttcgga atccccctgc ggggccggat
     2041 gaagggcagc aatctcaatt ttaataattc gatcgcctac gcccgcaatc ccagcatgct
     2101 gtataaccag atcaatgttt ccacgacctg ggtggttacc caaacggcgg ggatcaacct
     2161 ggtcttcaac gataagctta acctggggct gaatggcagc gtttcgtata accaggcggc
     2221 ctattccgtt caaaaaaacc tgaatgagga gtatttttca cagaattatt ccgccgatat
     2281 ttcgtacagc ttcataaaag actgggtatt ctataccgat tacagccagt atatcatcag
     2341 cggccgcgcc gccgggtata accagaccat tcctctctgg aacgcgagta ttgcccgcga
     2401 gatctttaaa aaacgaaacg gggagatcaa ggtatcggtc aacgacctgc tggaccaaaa
     2461 caaaagcatc acccggacgg tcggggataa ttacctgacc gatacccgct ccaatgtggt
     2521 gaaacgctat tttatgctca gtttcctgta taacctgaac cgttcgggga acggcgcaaa
     2581 aagaaacgac ggaatgcccc gcatgcccag gggaatgcaa agggaaatgc agcaattaag
     2641 atcggaaccg ggaggccgtc ccgcacccgg agcccaggcg gctcctcccc ccggccgccc
     2701 ataaaaaaac cgccggcggc ggtttgaatg aggtaaagac aggttatttt ttctttcgga
     2761 cataaggatg tgaagatcca tatggtgatt gtccttccac caggacgacc atgaccgttt
     2821 tgataaagtg gtatatatcg gtggtcggac gataactttt gcggtaaacc cgggctctct
     2881 gcgtttccag tctggcttgt attttcattg gttggtgctt ggaaagtaaa tatgggctat
     2941 tttaacgtcc cgggagcaga attattatgt aaaagcttgt taaacccggt ccgtattttt
     3001 gctattgtgc ttccctgaat tttatattaa tttagatccc aaatccaatt ccatgagaat
     3061 actgcttctg acctccgccc tgttgctgaa cacagccctg cttttttctc aggatgatgt
     3121 cttttccaat aagaccaacc aggcgcttga aaaagtgatc cgcgactatc ccaatcgttt
     3181 tcataatatc aaaggggaaa tgatctccca gcatgccaat acagccgaat acaaatcggt
     3241 ggtcatggtg cccggagcca gctcctgtac catttccaaa aacaatattt ccagcaccga
     3301 actctacagc tggagctgca cggcttttaa cagcagggat tttttggccg ccaggaacag
     3361 gtttaaggaa atctatgagc aggttgaaaa ttccattatt aaagtggatg ggcagaagcc
     3421 ctttatattg agcggccagt accgtacccc tacggaaatg cgcgatatga atgttatccc
     3481 gtttgaatta cttcccgccg tcggggacat gaaaaatcta aagatcgagc tctcgctgga
     3541 aaaaagcctg gccttctgga aggtaaaact caatgtttac gaaggggacc cgaagctcga
     3601 atcggccacg gcaaccgtgc agtaagcatt ataccagcag taaatattcc ctgagcaccg
     3661 cctcgatctc ttccagttta aacggcttgg atatataatt attcatcccg gcgtccaggc
     3721 atctttcctt gtcgcccttc atggcatcgg ccgtaagcgc gatgatcggg atccgggact
     3781 gggccgtctg cattttacgg atgatccggg tagtcgtata gccgtccatt tccggcatgt
     3841 tcacatccat gaaaatcagt acgggatcat agtgtggaag catttcgatc acctcccggc
     3901 cattctgcat ctggataatg tcaaagccca ttcttttcag tacttctgag atcagcatca
     3961 tattcacggg atcatcatcc accaccagga tgctggccga ctgggtgatt ttttcaaatg
     4021 attcaaaaga aggatggagg gaatcgttct gcatattctt ttcaaaaaga gagagcaggg
     4081 tgctgttcag ttcatggagc ttgaccggtt tggaaataaa cttatttatt ccggtcttgt
     4141 cggcttcatt ctggtaaagg tttttctcca gggaagaaag cattaaaata aaaggctgaa
     4201 tggcccccgg aatggaatgc ttgatttctt ttaccaggtt aatgccgtcc attcccggca
     4261 tatgatgatc tgtaatgatc agatcaaaag gtgctccgga agaattggcc atttcaatct
     4321 tcagcaacgc gtcgggtccg ttggtacagg tctcgcaata gatctggaaa taattcaggg
     4381 tttcctccat caggtgcagg ttggtaacat tgtcatccac caccagcacc tttttcaggg
     4441 aaggttttgg tttcagcagg atctccggct gatcattggc cacttccaaa ggcaggctga
     4501 gggaaaagac actcccgctc cccgcctcgc tttccaccga aagcgttccg tgcatcagtt
     4561 cggccaggct tttcgaaatg gcgagaccga gtccggtgcc tccgtatttc cgggtggtgg
     4621 aattatccgc ctgggtgaag ctttcaaaaa ttttctggag cttttctttc ggtattccga
     4681 tgccggtatc tttcacctgg atcacgaaat tctgatactt tttgccgtca acccagtaga
     4741 gctcggcttc tttccggatc gaaacataga tctcgccttc catcgtgaat ttgatggcat
     4801 tccccagcag gtttacgatg atctgccgga tacgaaccgg atcacccaat agctgggaag
     4861 ggatatcgtg atccacg
//
LOCUS       DASYCU010000280         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4119974, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000280 DASYCU010000000
VERSION     DASYCU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4119974"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VGM24_05595"
     CDS             complement(<1..551)
                     /locus_tag="VGM24_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY4154874.1"
                     /translation="MKIAVIGTGYVGLVTGTCFSETGNDVICVDIDSEKVKKLSQGEI
                     TIYEPGLNKLFLRNLKEDRLKFTTDLAAGVRDAEIIFLALPTPPGADGSADLRYVLGV
                     AGQLGSLLTDYKVIVDKSTVPVGTAEKVHDAVSKNFKGEFDVVSNPEFLREGVAVEDF
                     MKPDRVVIGTSSDRARKVMEELY"
     gene            complement(588..1715)
                     /locus_tag="VGM24_05600"
     CDS             complement(588..1715)
                     /locus_tag="VGM24_05600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY4154875.1"
                     /translation="MRPIQMVDLQGQYAGIKQQVDAAIHEVLETAAFINGKQVSEFSK
                     NLSAYLKIPHVIPCANGTDALQIALMGLGLEPGDEVITPSFTFIATTEVVALLGLKPV
                     FAEVDPKTFCLDPASVEKQITKKTKAIVPVHLYGQSAPMKEIMDIAGKYGLYVVEDNA
                     QAIGGDYHFPDGKSQKTGTIGTVGTTSFFPSKNLGAYGDGGALFTADESLADRFRMIA
                     NHGQKKRYYHELVGCNSRLDSLQAAVLNVKLPMLDNYILSRRKAADAYDLALGSHPKI
                     GIPYRAPYSNHVFHQYTILLKDADRNKLNEYLASKKIPSMIYYPVPAHRQQMFAAFHS
                     DRQKLEVTDWLTERVISLPMHTELDEEQIRFITNSVLEFLN"
     gene            1837..>2446
                     /locus_tag="VGM24_05605"
     CDS             1837..>2446
                     /locus_tag="VGM24_05605"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEY4154876.1"
                     /translation="MSILLYNLFLWLYRISVHLISPWNRKAAAWIQGRKGLLEKIGAS
                     GIGTARKRVWMHCASLGEFEQGRPVIESLRKKDPEITVLITFFSPSGYSVRKDYPGAD
                     YIFYLPADSALNARRFLDLVRPDLVLWIKYDYWFYFLTALRKRNIPALLISPLFREDQ
                     PFFKWYGNLHRLMLDSFRAFFVQTEASGKLLDGLGIRQNVIVS"
BASE COUNT          567 a          598 c          604 g          677 t
ORIGIN      
        1 ctgtataact cttccatcac ttttctggcg cggtcggacg aagtaccgat taccacccgg
       61 tcgggcttca tgaaatcttc caccgcaaca ccctccctta aaaattcagg gttggatacg
      121 acgtcaaact cccctttaaa attttttgat acggcatcat gtactttttc cgccgtcccg
      181 actggaaccg tgcttttatc gacgatcact ttatagtccg tcagcaggct gcccagctga
      241 cctgcaacac ccaatacata gcggagatcg gccgatccgt ctgctccggg tggcgtggga
      301 agcgccagga aaatgatttc cgcatccctg accccggcgg ccagatcagt cgtgaatttc
      361 aaccggtctt ctttcagatt ccgtaaaaac aatttattga ggccgggttc ataaatggtg
      421 atctcaccct gagacagctt tttcactttt tccgaatcga tgtccacaca gataacatca
      481 tttcctgttt cagaaaaaca ggttcctgta accagtccga catatcctgt tcctattact
      541 gcaatcttca taatctgata aggttttaaa gttcgggttt taagaaatca attcaggaat
      601 tccaatacac tattggtaat gaaccggatc tgttcttcat ccagttctgt gtgcataggg
      661 agggaaataa ctctttccgt gagccagtct gttacttcca gtttttgccg gtcggaatga
      721 aaagcggcaa acatctgctg gcggtgcgcc ggtaccggat aataaatcat ggaaggaatt
      781 tttttcgaag ccaggtactc attcagctta tttctgtcgg catccttcag caagatcgtg
      841 tattggtgga aaacatgatt ggaataaggt gcacggtacg ggatgccgat ttttggatgg
      901 ctgcccaaag ccagatcata agcatctgca gcttttcttc tgctcaggat ataattgtcc
      961 agcatcggca gtttgacatt cagcacggcg gcctgaaggg agtccagccg gctgttgcag
     1021 ccaacgagtt cgtggtaata tcgctttttc tgtccgtgat tggcgatcat ccggaaccgg
     1081 tctgccaaag attcatcggc ggtaaataaa gcgcccccgt ccccataggc tcccaggttt
     1141 ttggaaggga aaaaggaggt ggtgccgacc gtgccgatgg ttcctgtttt ctgcgattta
     1201 ccgtccggaa aatgatagtc gcccccgatg gcctgggcat tgtcttccac cacgtataat
     1261 ccatatttac ccgcaatatc catgatttct ttcatgggtg ccgactgccc gtataagtgc
     1321 accgggacga tggcttttgt ttttttggtt atctgttttt ccacggaagc cgggtcaagg
     1381 cagaaggttt ttggatccac ttccgcaaag accggtttta acccaagcag ggccacgact
     1441 tctgttgtgg cgataaacgt aaaagagggg gtaatgactt cgtcgccggg ttccagcccg
     1501 agtcccatca gggctatctg gagggcatcg gttccgtttg cacagggaat aacatgggga
     1561 atcttgaggt aagcggacag gtttttggaa aattcagaga cctgttttcc attgataaaa
     1621 gccgctgttt ccaagacctc gtggatggca gcatccacct gttgttttat ccccgcatac
     1681 tgcccctgga gatccaccat ttgaattggc cgcataatgc tcttcattaa cgggcacaaa
     1741 cctacattaa aataatggtt tatacaatga ctcaatcagt tatgaacggt ttagggaaag
     1801 gactacattt gtcggcagaa ccatctacca cccgatttga gtattttatt gtataacctt
     1861 tttctctggc tttaccggat ctcggtgcat cttatttcgc cctggaaccg gaaggccgct
     1921 gcctggattc aggggaggaa gggattgctg gagaaaatcg gggcttccgg gatcgggacc
     1981 gccaggaagc gggtctggat gcactgtgcc tccctgggag aatttgaaca gggaaggccg
     2041 gtgattgagt cgcttcgcaa aaaagacccg gaaatcacgg tattgatcac ttttttttcg
     2101 ccctccgggt attccgttcg taaagattac cccggagcgg attatatttt ttacctccct
     2161 gcggattcgg cgctgaatgc gcgtcgtttc ctcgacctgg taaggcccga tctggtgctt
     2221 tggataaaat acgattactg gttttatttt ctcacggccc tgcggaagcg gaatattccc
     2281 gcattgctga tttcgccgct tttccgggag gaccagcctt tttttaaatg gtatggaaac
     2341 ctgcaccgcc tgatgctcga cagtttccgg gctttttttg tgcaaaccga agcctccggg
     2401 aaactgctgg acgggttggg gatcaggcaa aatgtgatcg taagcg
//
LOCUS       DASYCU010000281         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1803093, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000281 DASYCU010000000
VERSION     DASYCU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803093"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VGM24_05610"
     CDS             complement(<1..599)
                     /locus_tag="VGM24_05610"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY4154877.1"
                     /translation="MKMVDSFEKIPLEIFPTARDGSAFVARELAALIRSRQEQKKTCV
                     LGLSTGSSPKQLYAELVRMHKEEGLSFRNVIAFNLDEYYPIERSAPQSYYQFMRKNLF
                     DHIDIDPANCHIPDGRVPKEAMKSHCAEYEHAIEASGGIDVQILGIGTNGHIGFNEPG
                     SSLFSRTRITPLENSTRLSNAGDFPSITDMPRLSVTMGI"
     gene            complement(772..1464)
                     /locus_tag="VGM24_05615"
     CDS             complement(772..1464)
                     /locus_tag="VGM24_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154878.1"
                     /translation="MEPSTSKQSAPLELWLKDVQSYIYKMTRPAMDRARFTFHRADYT
                     YRRKNKKSNDELSFIFLSRFPINYRVSFLLEIGHPQIRQVKESFMSEIMQKESNLCSI
                     VLNLRDFPSNDPQQEVVKDYSIYNQRDLFMVGDWLAQTLQYELIPLCDQMSTIEHMDA
                     FFESKPEWSLNTLGGGNVCTDLIVAKLNRKRDFEQRYEQLLQGLQQKIKQQLISPESR
                     QLLSLCYDTIKR"
BASE COUNT          395 a          365 c          401 g          427 t
ORIGIN      
        1 ctgatcccca tggttaccga aagccgcggc atatcggtaa tacttgggaa atctccggca
       61 ttcgaaagcc gggtggagtt ctccagcggg gtgatccggg tcctggaaaa aaggcttgaa
      121 ccgggttcgt tgaatccgat atggccgttt gttccgatcc caagaatctg cacatcgatt
      181 ccgcccgaag cttcaatggc gtgttcgtat tcggcacaat ggcttttcat ggcttccttg
      241 ggaacccggc catcgggtat atggcaattc gcagggtcta tgtcgatgtg gtcaaacagg
      301 ttcttccgca tgaattggta ataactctgg ggcgcacttc gttcgatcgg gtagtattca
      361 tccaggttga aagcgatcac attccggaaa ctcagcccct cctctttgtg catcctgacc
      421 agctcggcat agagttgctt cggggaagaa ccggtggaga gtccaagcac gcaggtcttt
      481 ttttgttcct gtctgctgcg gataagggca gccagctcac gggcaacaaa tgcagatccg
      541 tcccgggcag tcggaaaaat ctccagcgga atcttttcaa aagaatcaac cattttcatg
      601 tctgtgctct gttttgatgg aaattaggct gcaaaatact ttgtaattgg gcaatctcct
      661 aaaccgggtt ctccgggtat ttaataaaat ttttttctaa ctgaatgccg gggccggcga
      721 aaacggcaaa tggcgcgtca gggtaagtaa agataaaaag aaggctcctt tttatctttt
      781 aatcgtatca taacaaagag aaaggagctg ccggctttcg ggagagatca gttgttgttt
      841 aatcttttgc tgcaggccct gcaggagctg ttcgtaccgt tgttcaaaat cccttttccg
      901 gttgagtttg gcaacgatca ggtcggtgca gacgttgcct ccgccgagcg tatttaacga
      961 ccattccggt ttggattcaa aaaaggcgtc catatgttcg atcgtgctca tctggtcgca
     1021 taagggaatg agttcatact gcagcgtctg ggccagccag tcgcccacca taaacagatc
     1081 gcgctggtta taaatggaat aatcctttac cacttcctgt tgcggatcat tggacgggaa
     1141 atcccgaagg ttcaaaacga tgctgcataa attcgattct ttctgcatga tctcgctcat
     1201 aaacgattct ttcacctgac ggatctgcgg atgccctatt tccagcagaa aactgacgcg
     1261 gtaatttatg ggaaagcggc tcagaaatat aaaggacagt tcgtcgttgc ttttcttatt
     1321 cttgcgccgg taagtataat cagcccggtg aaaagtaaaa cgggcacggt ccatcgcagg
     1381 acgggtcatt ttgtagatat aggactgaac gtctttcagc cagagctcaa gcggggcgct
     1441 ttgtttactg gtggatggtt ccataaaggg aggtttgagc attcactacc agggacggca
     1501 attgaactac aaattaaata tcctcgctca aattttgaaa tatttatttt aaaaccaggc
     1561 cgtcctaacg gatgaccggc agctcaag
//
LOCUS       DASYCU010000282         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3976427, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000282 DASYCU010000000
VERSION     DASYCU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3976427"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VGM24_05620"
     CDS             complement(<1..1257)
                     /locus_tag="VGM24_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4154879.1"
                     /translation="MLKNFLKIAFRNLWKNKGFTVINITGLAIGMASAMLLLLWIHDE
                     LSFDQFHEKKDRIYEVWNRANFSGELHCWNTTPKILARTMEKDFPEIEHSVRVNWPSN
                     YLFSVGEKRLMVQGNIVDSPFLDVFSFPLISGNPHTALNDMHSIVITQKLSKKLFGKE
                     DPMGKLIKIDNNETFRVTGLARDLPGNTRFDFEYLIPWAYLHHIGGDDESWGNNSTRT
                     YVLLKPRMNPANLVQRVKKLKVKYDPSDPKWEMFLYPMNRWRLYSAFNGGAEEGGRIE
                     FVKLSALVAAFILLIACINFMNLNTARSEKRAREVGIRKVVGARRGSLISQFLGESIL
                     MSAMAAVLAFGLVQLSLPAFNRITDKTLSIPYGSGGFWLLVIAFILFTGLIAGSYPAF
                     FLSSFQPVKVLKGTFNKVHALVTPRKL"
     gene            complement(1457..>1911)
                     /locus_tag="VGM24_05625"
     CDS             complement(1457..>1911)
                     /locus_tag="VGM24_05625"
                     /inference="COORDINATES: protein motif:HMM:NF028498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEY4154880.1"
                     /translation="NLYFTEQMVIEDHGFQIAAEVNRLIIGATRNWSRNVLLWNLAAD
                     KDNRPHTDNGGCTMCQGAVTIEGNEVSRNLAYYVIAQASKFVHPGSVRIGSRQTEHLT
                     DVAFRTPEGKTVLIVANNTVADQPFTVQVSGKAFSGNLEAGAVGTYFW"
BASE COUNT          495 a          489 c          458 g          469 t
ORIGIN      
        1 caattttctg ggagttacga gcgcatggac cttgttaaag gttcccttga gcacttttac
       61 cggctgaaag gacgaaagga aaaaggctgg ataactgccg gctatgagtc cggtgaacag
      121 aatgaaggcg atgaccagca accagaatcc gccgcttcca tagggaatgg acaaggtctt
      181 atccgttatc ctgttaaagg caggcaaact aagctgtacc aacccgaaag ccaggaccgc
      241 ggccatagca ctcatcagga tggattctcc caggaactgg ctgatcaggg aaccgcggcg
      301 ggcccccact actttgcgaa ttccaacctc tctcgccctt ttctcgctcc ttgccgtatt
      361 caggttcatg aaattaatac aggcaatgag caaaatgaat gcagcaacca gggctgagag
      421 tttgacaaac tcaattcttc cgccttcttc cgcacctcca ttgaaagcgg aatacaacct
      481 ccagcggttc atcgggtaaa gaaacatttc ccatttggga tcgctggggt catattttac
      541 tttcagcttt tttacccttt gcaccaggtt ggccggattc atccggggct tcagcaacac
      601 ataagtgcgg gtggaattat tgccccagga ttcatcgtcc ccgccgatat gatgtaaata
      661 ggcccagggg atcaggtatt caaagtcaaa ccgggtgttc cccggaagat ccctggccag
      721 cccggtcacc cggaaggttt cgttattgtc aatcttgata agtttcccca tggggtcttc
      781 cttcccgaat aattttttag aaagtttttg tgtgatcacg atggaatgca tatcgttcag
      841 ggcggtatgc ggattcccgc tgatcagcgg aaaactgaat acgtccagga aaggagaatc
      901 cacgatatta ccctgcacca tcagcctttt ctctcccaca ctgaataaat aattggacgg
      961 ccagttgacc cgtaccgaat gttctatttc gggaaagtcc ttttccattg tccgggccag
     1021 tattttcgga gtcgtattcc agcaatggag ttctccgctg aagttggccc ggttccagac
     1081 ttcatagatg cggtcctttt tttcatgaaa ttgatcaaaa ctcagctcat catggatcca
     1141 aagcaaaagc agcatcgcac tcgccatacc gatcgccaga ccagtaatat tgattaccgt
     1201 aaatccttta ttcttccaga gattgcggaa agcgattttc aaaaaattct ttagcatttg
     1261 agcaggattt taatccggtt tggagaacag gctgtaagct gaaaaaggag aactgccgga
     1321 aaagcccggg ctggatcgct cccgcagatt cagaaagccc cggaattaag acacaggcaa
     1381 ttgcagaatg ttgcaggagg aaataaaagg aagaaaaaaa gcaggccgga taaaacgggg
     1441 tttccctcag ccctgctcac cagaaataag tgccgacggc gcccgcctcc aggtttccgg
     1501 aaaaggcttt cccggaaacc tgcacggtga acggttgatc agccaccgta ttgttggcaa
     1561 cgatcagcac tgttttgcct tcgggtgtgc ggaaagccac atcggttaaa tgttcagtct
     1621 gacgcgaacc gatacggact gaacccggat gcacaaattt tgaagcctgg gcaattacat
     1681 aataggcgag atttcgggaa acctcgttcc cttcaatcgt cactgccccc tggcacatgg
     1741 tgcaaccgcc gttgtcggtg tggggcctgt tatctttatc tgccgcgagg ttccatagca
     1801 gcacattcct gctccagttg cgggtagcgc caatgatcag tcggttcacc tccgccgcaa
     1861 tctgaaatcc gtgatcttca atcaccatct gctctgtgaa ataaagattc t
//
LOCUS       DASYCU010000283         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5137322, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000283 DASYCU010000000
VERSION     DASYCU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5137322"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..484
                     /locus_tag="VGM24_05630"
     CDS             35..484
                     /locus_tag="VGM24_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154881.1"
                     /translation="MNSIHPSEKEIQSYVLDKSGCSTELVAHMESCEDCRTDVATYQL
                     LFSEIKQQPKPAFDFDLRALVLPQLPSSTPRLSTDRFVAGFLVVFLCGCVGIPVYIFR
                     QNFLYLFSGLSTFFIWAILCSALLILLFKALNMYKKYQKQMHFLNFN"
     gene            546..1004
                     /locus_tag="VGM24_05635"
     CDS             546..1004
                     /locus_tag="VGM24_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154882.1"
                     /translation="MKKLLSTAIILGFVFVAQAQDPFDPLRDRQLYFDSLNILGILTA
                     MYLISSFILQIFRQHYSFRIKNRMLDKGTEENVVRQMLQPDKKENKNYVLQWFFTLGA
                     IGVGLILVTLVRPYGVRSVAILALSVAAGFGAYYYFTRQNEKINETGTGR"
     gene            1028..2233
                     /locus_tag="VGM24_05640"
     CDS             1028..2233
                     /locus_tag="VGM24_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154883.1"
                     /translation="MKLIFFLVAFCLVGIRVYFSWLGKDKQRRGETEYNMNIVSDNIH
                     EEINYSGKFGLTDDETGFKTISPGGYMKFRMNDVKVKAESSLQGKIEYRIYDGKDQLS
                     AEDESGKKLIAKAIREMIGWGFDANARMERVYRKGGIPALLSEADSMRSDDVKAMYLD
                     RLISIDSLSSGDLLEVAKKIGSLGSDMGKASLLNKFSAARLKDPQIDSVYFKVVSGMG
                     SDMDKVNTLRHMMDQDSLEEGNVYQILALAGHMGSDMDKSNLFGQLMDKGLIQGARFD
                     SLLNLISDMGSDMDKVNLYSRLIEHQNITEPQWTALLNEASRLGSDMDKINMYKKLME
                     EKAITESQWEGLLDKTAQLGSDMDKANLLTEIARKMPKDENLKIAYMKSAKSIGNDSD
                     YGRVIRAME"
     gene            complement(2514..3086)
                     /locus_tag="VGM24_05645"
     CDS             complement(2514..3086)
                     /locus_tag="VGM24_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154884.1"
                     /translation="MKNGLFVLLLAPLLCFSQKKNGPTLQAGGGYLFYPGSSADVHIA
                     GDYPGNPLKSGLTFELTADQPVNSFLSLGLGSSFIHFKNLGAPYIPVFADIRVVGVGK
                     YKLFSFLDPGYGIYHHGFTDDSAGAGPQPGKRSGGFYIAYGFGVIYHIFYLQAKYNWL
                     RFKTETAGSDGENSSKAYGVGGITLGVRLP"
     gene            3199..>3395
                     /locus_tag="VGM24_05650"
     CDS             3199..>3395
                     /locus_tag="VGM24_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154885.1"
                     /translation="MLKFLLFGFCVFSLASSRAQDKKSDTTDSRRILPERTISFSGYK
                     WTVRNIAGKQGPGPNYFNDSSV"
BASE COUNT          932 a          774 c          826 g          863 t
ORIGIN      
        1 aaaacttatt gtcaaaatat aaaaaggaag ccttatgaat tccatccacc cgtcagaaaa
       61 agagattcag tcatacgtac tggataaatc cggttgctca acggaacttg ttgcgcatat
      121 ggaatcctgc gaagattgca ggacggatgt ggccacttac cagttgttgt tttctgaaat
      181 aaaacagcag cccaagccgg ctttcgattt tgatctgcgc gcgctggtcc ttccccagtt
      241 gccgtcctct acgccgcgcc tgtcgacaga tcgttttgtc gccggtttcc tggttgtttt
      301 cctttgcggc tgtgtcggca ttccggttta tattttcagg caaaattttt tatacctgtt
      361 ttccggtctt tcgaccttct tcatttgggc catcctttgc agcgccctcc tcatcctttt
      421 gtttaaagcc ctgaatatgt ataagaaata ccagaaacaa atgcactttt tgaatttcaa
      481 ttagattttg aaacattata tgctcatccc ggtcaaatag ttatgttcca cttcaatcaa
      541 taaaaatgaa aaaattactc agcaccgcaa tcattttagg tttcgtgttc gtagcccagg
      601 cgcaggatcc ttttgatccc ttaagagatc gtcaactgta ttttgacagc ctgaatattt
      661 tgggcatatt gacagcgatg tacctgatct cttcttttat cctgcagatt ttcaggcagc
      721 actacagctt ccgtatcaaa aacaggatgc tcgataaggg aacagaggaa aatgtggtcc
      781 ggcaaatgct tcaaccggat aaaaaagaaa ataaaaacta cgtcctccaa tggttcttca
      841 cgcttggcgc catcggtgtc ggattgatcc tggtcacgct ggttcgtccc tatggtgtcc
      901 gctccgtggc catcctggct ttaagtgtgg cggccggctt tggcgcttat tattatttta
      961 ccaggcagaa cgagaaaatc aatgaaacag gtaccggccg ctgatcccgg acaataaaaa
     1021 tatacagatg aaactcattt tttttctggt cgccttttgc ctcgtgggta tccgggttta
     1081 tttttcctgg ttaggaaagg ataagcaaag aaggggagaa acggagtaca atatgaatat
     1141 cgtctctgat aatattcatg aggaaattaa ttattcggga aaattcgggc taacggacga
     1201 cgagaccggt tttaaaacta tttccccggg agggtatatg aaattccgta tgaatgacgt
     1261 gaaggtaaag gcggaaagca gtctccaggg aaagatcgaa taccggatct acgatggaaa
     1321 agatcaactg agcgcggagg atgaatccgg caaaaagctg atcgctaaag ctatccggga
     1381 gatgatcggc tggggcttcg atgcaaatgc acggatggaa cgcgtttacc gcaaaggagg
     1441 aatcccggcc ttgttaagtg aagccgattc catgagatca gacgatgtta aggcgatgta
     1501 cctggatcgc ctgatttcca ttgactccct ttcatccgga gacctgctgg aggttgcaaa
     1561 aaaaatcgga agcctgggtt ccgacatggg taaggccagc cttttaaata aattttcagc
     1621 agcccggctt aaagatccgc aaatcgattc ggtttatttt aaagtggtgt cgggaatggg
     1681 atccgatatg gacaaggtaa atacccttcg gcatatgatg gatcaggaca gcctagagga
     1741 aggaaatgta tatcagatac tcgccctggc cgggcatatg ggctcggata tggataaatc
     1801 aaatcttttc gggcagttga tggacaaggg cctgattcag ggagcacgtt tcgacagcct
     1861 gctgaatctc atatccgata tgggatccga tatggataaa gtaaacctgt atagcaggct
     1921 catagagcac caaaacatca cggagccgca atggacggcc ctgttgaacg aggcttcccg
     1981 gttgggctcc gacatggata aaataaacat gtataaaaag ctgatggagg aaaaagccat
     2041 caccgaatcc caatgggaag gcctgctgga caaaacggca caattgggtt cagatatgga
     2101 caaggccaat ctcctgacgg agatcgcccg gaaaatgcct aaggacgaaa acctgaaaat
     2161 agcttatatg aaatcggcca aatcaatcgg caacgattcg gattacggcc gggtgatccg
     2221 ggcaatggaa taaatgggca gaagggttcc tggaaacagc aatgcccttt gcctccgccc
     2281 ggcaaaaaat tcctaccgga tgatcagagt ggtcatcgcc agggccagaa aggccagggc
     2341 cagaagaata ctttttttca taaacaggtt ttaatggatt tcccttttat gaaaataagc
     2401 agatccggaa gcggcggcgc ctgcagtttg ccggcggccg gatacagcct gtgaccggtc
     2461 gtttttagta tgtaccgggc ggatggcccc gctccttcta taaatataat ttatcaggga
     2521 aggcgaacgc ccagggtaat gcctccgacc ccgtaggcct tactggaatt ttccccgtcc
     2581 gaaccggccg tttccgtttt aaaacgcagc cagttgtatt tggcctgcag gtagaaaata
     2641 tgataaatga cgccaaagcc gtaagcaata tagaaacctc cggaccgctt tccgggttgc
     2701 gggcccgccc ccgctgaatc gtcggtgaaa ccgtgatgat agatcccgta accgggatcg
     2761 agaaaagaaa ataatttgta tttcccgacc cccactaccc ggatatcggc aaatacgggt
     2821 atatagggag cacccaggtt tttaaagtgg atgaaggagg atcccagtcc gagagacaaa
     2881 aaggaattca ccggctggtc tgccgtgagt tcaaaggtaa gcccggattt cagcgggtta
     2941 cccggatagt cgccggctat atgaacgtcc gccgaagaac cggggtagaa gagatatccg
     3001 ccaccagcct gcagcgtggg cccgtttttt ttctggctga aacaaagcaa aggcgcaagg
     3061 agaaggacga aaaggccatt tttcatcccg tatgaatttc caataaatcc ggatttaacc
     3121 atattttgcc gccttcgttt ccgtttcctt ttcttaaaat gctaaattag tactcaatcc
     3181 ctcagtacaa tcttacttat gttaaaattt cttttatttg gtttttgtgt tttcagtctg
     3241 gcctcttccc gcgcccagga taaaaaaagc gatacaacag attctcgccg catccttccc
     3301 gaaaggacca tttccttcag cggatataaa tggacggtgc ggaacattgc cgggaagcag
     3361 ggaccgggtc cgaattattt caacgattcc tcggt
//
LOCUS       DASYCU010000284         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2672934, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000284 DASYCU010000000
VERSION     DASYCU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672934"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VGM24_05655"
     CDS             complement(<1..860)
                     /locus_tag="VGM24_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154886.1"
                     /translation="MKRRELIKQGAFLGLAAHPLAKAFGQKPKSSLAFFRNAAFNGLV
                     KPVFVYNNWSAYDELSDKVAQTELLALRELDEMIRLKRSGVQLDYYVMDAFWFDKMGG
                     YRSWNKEHWPDGPGKWLAGCRQNNILPGMWFSTNLIATHSGRFLEVIDAWKDSLATDP
                     NILCLFEGGYLDHLAGSLQLWYDQGVRLFKFDFAYFEAVTPASKNKYTAEQIQEKNKL
                     AFIGMLQKFRYQNPDVIITGYNGFGGDMENTFTPFKKDINLRWLEVFDTLYCGDPRFS
                     DVPMMNIWRSA"
     gene            complement(880..2457)
                     /locus_tag="VGM24_05660"
     CDS             complement(880..2457)
                     /locus_tag="VGM24_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY4154887.1"
                     /translation="MSIGYFKMGYSAFFAIGALINSGEKSGRQPGQTSPSEYYFSASG
                     NDQNDGSRDHPFNSISKLNGIHLQAGDAVYFHGGDTFDGSVLIDSSSKGTTASQIIIS
                     SYGKGKATIHGGNGKAFTVYETQYITVNNLNLKGSGRKGGNLKEGLAVLNSDHITVTD
                     LDISGFQKSGLLVYVSSNVAVSRVYTHDNGSAGITVEASQGKKESRNIQIRYCRAENN
                     PGDPTNLNNHSGNGIVAGHCTNLLIEYCTATNNGWDMPRIGNGPVGIWCYESDSAVIQ
                     HCLSYRNKTSVGGADGGGFDLDGGVTNSIVQYNLSYENQGSGYCIFQYWGASPWYNNV
                     FRYNISENDGSVSDSRAGIYVWNSSEDAGQFHDCLVYNNSIYNSKEAAISFSDKSARK
                     QFGFYNNIFVGKDSLIKGDKGEDIFLGNDWWSLADQFNADGMHSFPAWAKKFQKEMIS
                     GKIAGFNLDPHFREPGKMSLPSFPHPAGAFSEYKIPAKSVLRNRGIDLHRRLNIDTGN
                     RDFGGAAAPEKGIGASF"
     gene            complement(2524..>2901)
                     /locus_tag="VGM24_05665"
     CDS             complement(2524..>2901)
                     /locus_tag="VGM24_05665"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4154888.1"
                     /translation="LENARALVNRVRSRAINGCQVLNDLGDPAANYFAGTYDSPWSDQ
                     NFARNAIRLESRIELSLEGHRFFDLVRWGIAGDYLNAYFQKEQTRIDHLQNVKFTAQK
                     NEYFPIPEKEIGLNHHLVQNSGY"
BASE COUNT          717 a          759 c          654 g          771 t
ORIGIN      
        1 gccgagcgcc agatattcat catcggcaca tcggaaaacc gcgggtcacc gcaataaagg
       61 gtatcaaaca cttccagcca gcgcagatta atatcctttt tgaacggggt aaacgtattt
      121 tccatatccc caccgaaacc attatacccc gtgatgatga catccggatt ctggtaccgg
      181 aatttttgga gcatgccgat gaatgccagt ttatttttct cctgtatctg ttcggccgtg
      241 tacttgtttt ttgaagccgg cgtcaccgct tcaaaataag caaagtcaaa tttaaataac
      301 cgcaccccct gatcgtacca aagctggagc gaaccggcca ggtgatccag gtaacctcct
      361 tcaaacaaac aaaggatatt cggatctgtt gccagggaat ccttccaggc gtcgatcact
      421 tccagaaatc ttccgctatg ggtagcaatg aggttcgtgg aaaaccacat gccgggaaga
      481 atattatttt gcctgcagcc ggcgagccat ttccccggtc cgtccggcca atgctccttg
      541 ttccagctgc ggtatccgcc cattttatcg aaccagaacg catccataac ataataatcc
      601 agttgcacgc cactccgttt taaccggatc atctcatcca gttcccgcaa ggccagcagt
      661 tctgtctgcg cgaccttgtc cgacagttcg tcgtatgccg accaattgtt ataaacgaaa
      721 accggtttaa caagaccatt gaatgccgca ttgcgaaaaa aagccaatga tgattttggc
      781 ttttgtccga aggccttcgc taacggatgt gcagccaatc ctaaaaaagc tccttgtttg
      841 atcagttctc ttcttttcat cagaatttat gaattggttt caaaaactgg cgccgatccc
      901 tttttccggg gctgccgcgc cgccaaaatc ccggttaccc gtatcgatat ttaaccggcg
      961 gtgcaaatcg atgccacggt tgcgaaggac agatttcgcc ggaattttat attcggaaaa
     1021 tgctccggcc ggatggggaa aggaggggag gctcatttta ccgggttccc ggaaatgcgg
     1081 atccaggtta aatccggcta ttttcccgga gatcatttcc ttttggaatt tctttgccca
     1141 cgcggggaat gaatgcattc cgtcggcatt aaattggtct gccagactcc accagtcgtt
     1201 gcccagaaaa atatcttctc ctttatctcc tttgatcagg ctgtcctttc cgacaaatat
     1261 attgttataa aatccgaact gcttcctggc cgatttatca gaaaagctga ttgccgcttc
     1321 ctttgaatta taaatgctgt tgttgtaaac gagacagtcg tgaaactgac cggcatcttc
     1381 cgaactgttc cagacatata ttccggcgcg ggaatcggaa acggatccgt cattctcact
     1441 gatattgtac cggaaaacgt tattatacca gggactggca ccccaatatt gaaatatgca
     1501 atagccgctt ccctggtttt cgtacgacag gttatattga acgatggagt tggttacgcc
     1561 accatccaga tcaaagccgc cgccgtctgc cccgccgacg gaagttttat tgcggtagga
     1621 gaggcagtgc tgaatgacgg cgctgtccga ttcataacac cagatgccca ccgggccatt
     1681 tccgattcgc ggcatatccc aaccgttgtt tgttgccgta caatattcaa tgagcagatt
     1741 ggtacagtgg cctgccacga taccattgcc actgtgatta ttcaggttgg tcggatcgcc
     1801 cggattattt tccgcccggc aatagcggat ctgaatattc cgcgattcct tttttccctg
     1861 actggcctca acggtgatcc cggcggaccc gttatcgtgg gtgtaaaccc gggataccgc
     1921 tacattggaa gagacataaa ccagcaggcc ggatttttga aacccggaaa tgtccaggtc
     1981 agtaaccgta atatgatcgc tgttaagcac ggccagaccc tccttcagat tgccgccctt
     2041 tctacccgaa cctttcagat tcagattatt tacggttata tattgagttt cgtaaaccgt
     2101 aaatgccttt ccgttcccgc catggatagt tgctttcccc ttgccatagg agcttataat
     2161 aatttggctg gccgtagttc ccttactgga agaatcaatc agcacactgc cgtcaaacgt
     2221 atcgccgcca tggaaataga ccgcgtcccc tgcctgcagg tgaataccat tgagtttgga
     2281 aatggaatta aacggatgat cccggcttcc gtcgttctga tcgttccccg atgcgctgaa
     2341 ataatactcg ctgggactcg tctggcccgg ttgcctgcct gatttttccc cggaattgat
     2401 cagcgcgccg atggcaaaga aagcagaata acccatttta aaatatccaa tgctcatccg
     2461 gatgaaaatc atttgagctg taaaaaaaag ctgttggcac ctaaacagtc cggcatgcta
     2521 atcttaatac ccgctgtttt gtaccagatg atgattcagt ccgatttctt tttcgggaat
     2581 cggaaagtat tcattcttct gagcagtaaa ctttacattc tgcaaatggt cgatgcgggt
     2641 ctgctctttc tgaaaatacg cattcagata atctcccgca attccccagc gcaccaggtc
     2701 aaaaaaccgg tgaccttcca gcgacagttc gatccggctt tccaggcgaa tggcgttacg
     2761 ggcaaagttt tgatctgacc agggggaatc gtatgttccc gcaaaatagt ttgctgccgg
     2821 atcaccgaga tcattcagga cctggcatcc gttaatggcg cggcttctga cccggtttac
     2881 caaagcacgg gcattctcca g
//
LOCUS       DASYCU010000285         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2672986, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000285 DASYCU010000000
VERSION     DASYCU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672986"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VGM24_05670"
     CDS             complement(<1..953)
                     /locus_tag="VGM24_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4154889.1"
                     /translation="MNLKTNISGFAFGLLMLVSVAGMAQDSVRNISLDEAIGLSIKNS
                     KQLRASKAKMEEASAALTESKQKQLPDLKISGAYLRVTQPNLDLKLKLNNNNGGSGGS
                     SPSEIKVDQAAYGIANLSLPLYSGLRIQYGIRSAEFLEKAATLDADNDKEAIILNTIS
                     AFSNLYKAGVNVRLIRENLAQSRSRDSDFNNLERNGLLARNDKLKAALQTSNIELNLV
                     DAESNLQVAMVNMNLLLGLPEHGQIIPDSSSLVQSASLKGIDDYEQLAVQNRKDVQAM
                     QYRKKASAVGIQSAKSDYYPSLALTGGYIAADIPNLVTITNA"
     gene            complement(943..1578)
                     /locus_tag="VGM24_05675"
     CDS             complement(943..1578)
                     /locus_tag="VGM24_05675"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY4154890.1"
                     /translation="MKSEYSAKQLQLLDTAELLFSRKGFEGTSVRDIAEEAGINTAMI
                     SYYFGSKEKLMEAIFERRSLNIKEKVDLLMKDDSLDPFQKMFSLLDEYIDRILQRKKF
                     HRILLCEQVINQNPAILEMLSKLKIRNSASINELIRAGQKKGLFKKQVDVPMLMNTLV
                     GTITHILVNLEFYKNYHNLESLSDEEFERILKKKLSVHIKIIFKAILSHES"
     gene            1719..>3407
                     /locus_tag="VGM24_05680"
     CDS             1719..>3407
                     /locus_tag="VGM24_05680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEY4154891.1"
                     /translation="MKFFAFLLTCASLLFFSCQGPSENAGIKTATDAIDFSANDHTVR
                     PQNNFFLYVNGEWLKNTVIPPTQSSWGTFSVLQDSSVARLHGILDSLVNLSNLTKGSI
                     AQQTADLFYSAMDSAGIETRGFDPVRPELERIENLKNIKDLMNEVATEYQVNHSPFFS
                     FYVNADDRNSRMNIAHFDQGGLGMPSRDYYFKNDSSTVKVRNGYKSYISRIFTLTGDQ
                     PADADKKAGGILGLETTLAGVSRGPEEFRDPVANYHKLTVAGLNKTLPGFGEMLENLG
                     VHADTVLMGQPAFYEGLYAAFRKTPLQTLKDYLCFHVLNDDADYLSHDFAAARFGYDK
                     LLTGQSAPKERWKRMTSLIDNQLGDALGQFYVQKYFPPAAKQRINELVDNIVTTFGER
                     LQKLDWMSDSTRKKAIIKLHAIVKKVGYPDKWKDYSSVNIVRDDIISNLKATANYGYR
                     RQIDKIGKPVDRSEWVLTPPTINAYYNPTANNINFPAGILQPPFYFVSGDDAVNYGAI
                     GMVIGHEMTHGFDDKGRQYDADGNLKDWWTAEDAKKFKQRAQKIIDQYNGYIAID"
BASE COUNT          893 a          839 c          777 g          898 t
ORIGIN      
        1 gcattcgtga tcgtgaccag gttcgggata tctgccgcta tatagccacc ggttaaggcc
       61 agggacggat aataatcact tttggccgac tggatgccaa cagcagaggc cttcttccgg
      121 tactgcatgg cctgtacgtc cttacggttc tgaacggcca gttgttcgta gtcatcgatt
      181 ccctttagtg aagcggactg aaccaggcta ctcgaatcgg gaatgatctg tccgtgttcc
      241 ggaagcccga gcaaaagatt catattcacc atggccacct gcagattgct ttccgcatcc
      301 accaggttca actcaatatt cgaggtttga agggccgctt ttaatttatc attccttgcc
      361 agcaaaccat ttcgttccag gttattgaaa tccgaatccc ttgacctgga ttgagccagg
      421 ttttcgcgga tcaggcgcac attgaccccg gccttgtaga ggttgctgaa ggccgaaatg
      481 gtattcagaa tgatcgcctc tttatcattg tccgcgtcca gcgtagccgc tttctccaaa
      541 aattctgccg aacggatccc gtattgaatg cggagaccgg agtataatgg cagcgaaagg
      601 ttggctattc cataggcggc ctggtctacc tttatttcac tcggcgaact gcctccggaa
      661 cccccgttat tattgttgag cttcagtttc agatcaaggt tcggctgtgt taccctcaga
      721 taagccccgc ttattttcaa atcgggcaat tgcttctgtt ttgattccgt cagggcagcc
      781 gatgcttcct ccatcttcgc tttggaggcc cgcagctgtt tactgttttt aatactcaat
      841 ccgatggcct cgtccaggga aatgttccgg acactgtcct gggccattcc ggcaacggat
      901 accagcatca gcagtccaaa ggcaaacccg gaaatatttg ttttaagatt catgcgaaag
      961 aattgctttg aaaatgattt taatgtgcac cgataatttc ttcttgagta tacgttcaaa
     1021 ttcttcatcc gaaagagatt ccaggttatg atagttcttg taaaattcca ggttgaccag
     1081 gatatgcgtt atcgttccca ccagggtatt catcagcatc gggacgtcca cctgcttctt
     1141 gaagagccct tttttctgcc cggcacggat cagttcattt atactcgccg aattccggat
     1201 cttcaatttg gacagcattt ccagaatggc cggattctga ttgatcacct gttcacacaa
     1261 caggatgcgg tgaaactttt tacgttgcag gatccggtca atatattcat ccagcaggct
     1321 gaacattttc tggaacggat ccaggctgtc atccttcatc agtaaatcca ccttctcctt
     1381 gatattcagg cttcttcgtt caaaaatcgc ctccatcagt ttctccttcg agccaaaata
     1441 ataggaaatc atcgccgtgt tgatgccggc ttcctctgcg atatcccgta ccgacgtgcc
     1501 ttcaaacccc tttcgggaga ataacagctc cgccgtatcc agtaattgca attgcttcgc
     1561 agaatattct gatttcatgg gatgcaaaat taattaaacg tttgtttaat ttcctaatta
     1621 ttcggaaaat aatttttttt ttggaacgcc ggcattgatt gaaaacatga tttccaacag
     1681 gcaataatca ttactttcac tcctaacaaa atttatttat gaaatttttt gcttttttac
     1741 tgacctgtgc ttccctgctt tttttctcct gccagggacc ctcggagaat gccggaataa
     1801 aaacagcaac agatgcgatt gacttttcag caaatgacca tacggtcaga ccacaaaaca
     1861 atttcttctt atatgtaaac ggagaatggt taaaaaacac cgtcatcccg cctacccaat
     1921 cctcctgggg tacgttctcg gttctgcagg atagttccgt agcccgtctc cacggcatcc
     1981 tggactccct ggtgaactta tccaacctga cgaaagggtc tatcgcacag cagacggccg
     2041 acctgtttta cagcgcgatg gattccgccg gtattgaaac ccggggtttt gatccggtca
     2101 gacccgaact ggaaagaatt gaaaacctta aaaatataaa ggacctgatg aatgaagtgg
     2161 caacggaata ccaggtgaac cacagccctt tcttttcatt ctatgtgaac gcagacgacc
     2221 ggaacagccg gatgaatatc gctcattttg atcagggcgg tctgggaatg cccagccggg
     2281 attattattt taaaaacgac agctccaccg taaaggtcag gaatggatat aaatcctata
     2341 tcagcaggat ttttaccctc accggcgatc agccggcgga cgcggataaa aaagccggcg
     2401 gcattctggg cctcgaaacc acgctggccg gagtgtccag gggtccggaa gagttcaggg
     2461 atccggtggc caactaccat aagctgacgg ttgccggcct gaataaaacc cttcccgggt
     2521 tcggagaaat gctggaaaac ctgggtgttc acgctgatac ggtattgatg gggcaacccg
     2581 ccttttacga aggcctctat gccgcattcc ggaaaacccc gctgcaaacc ctgaaagact
     2641 atttgtgctt tcatgtgctg aatgacgacg cggattatct ttcgcatgat ttcgcggcag
     2701 cgcggttcgg ttatgataaa ttactgaccg gacaatccgc cccgaaagaa aggtggaaaa
     2761 gaatgacctc tctcatagac aaccagctcg gagacgccct gggccagttt tatgttcaga
     2821 aatattttcc gccggcggca aaacaaagga tcaatgaact ggtggacaat atcgtcacca
     2881 cgtttggtga gcgattgcag aaactggact ggatgagcga ttccacccgg aaaaaagcca
     2941 ttataaaatt acacgccata gtcaaaaaag taggataccc ggataaatgg aaagattatt
     3001 cttctgtaaa tattgtccgg gatgatatta tttctaacct gaaagccacc gcgaattatg
     3061 gctacagaag gcaaatcgat aagatcggaa agccggtgga ccgctccgaa tgggttttga
     3121 ccccgcccac catcaatgcg tattataatc cgacagccaa caatatcaat ttcccggcgg
     3181 gaattcttca gccgcctttt tattttgttt ccggggacga cgccgtaaac tatggtgcga
     3241 tcggaatggt gatcggccat gaaatgactc atggattcga tgacaagggc cgtcaatacg
     3301 atgcagatgg taatctgaaa gactggtgga cggcagaaga tgcaaaaaaa ttcaaacaac
     3361 gcgcacagaa gatcatagat cagtacaacg ggtacatagc catcgac
//
LOCUS       DASYCU010000286        10384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_499464, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000286 DASYCU010000000
VERSION     DASYCU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499464"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..465
                     /locus_tag="VGM24_05685"
     CDS             208..465
                     /locus_tag="VGM24_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154892.1"
                     /translation="MEISSHPEKFSNDIIVEIRTESEDHCIIVLTNHSGRIRRMMGVN
                     LNQGQNDIHVENVDTLEAGLYQLSVKNTDSTLLYTSILTKS"
     gene            complement(578..1843)
                     /locus_tag="VGM24_05690"
     CDS             complement(578..1843)
                     /locus_tag="VGM24_05690"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY4154893.1"
                     /translation="MKKIFTLLFVLPILYFSADGQIILNGQAVSRDIDYARLRRIDTL
                     VNGYVQKGWVKGVATIIVQDNHLIQFKGYGFLDNAAKKPLPPNALFRIASQTKAVVSV
                     GAMTLYEEGKFMLDEPIADFIPEFRHPQVLDHFNEKDSSYTSRPAKREITFRDLFTHT
                     SGLDYPGIGSPSMKAIYAKAGIPSGLGVENMDLLETVKRLAKLPLVHDPGEKWTYGLS
                     VDVLGCLVEQISGKDLEDYLAEKIFKPLGMKDTYFNVPATKADRLTTVYTEDSLGHVL
                     LWDKNKTGIDPDYPIVKKHYFSGGAGLTSTAYDYAVFLQMLLNKGKYNGVQVLAPRTV
                     EMMTSGQLDHPFDGIDNFGLGFEITGEKSAARGPRNIGTFSWGGFFGTTYWADPKAKL
                     VCLIMTQQTPNQHGDLSTKFEQLVYQSLK"
     gene            1967..2689
                     /locus_tag="VGM24_05695"
     CDS             1967..2689
                     /locus_tag="VGM24_05695"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY4154894.1"
                     /translation="MIRLSVNINKFATLRNSRGGNNPDIVKAAVDAQRFGADGVTVHP
                     RPDERHIRYADVFAIKKSISTEFNIEGNCQEQKFIDLVLKAKPDQVTLVPDALDQITS
                     NHGWDTIQAADFLREIIPVFKQAGIRVSIFVDPVERMVEGAALTGTDRVELYTEGYAK
                     TFPENKEKAIAPYLSAAKKAKSLGLGINAGHDLDLDNLHYFARSIPFLDEVSIGHALI
                     CDALYLGYENAVQLYKRQLQID"
     gene            complement(2711..3808)
                     /locus_tag="VGM24_05700"
     CDS             complement(2711..3808)
                     /locus_tag="VGM24_05700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY4154895.1"
                     /translation="MKRVLIILTALAGVARAQNAKPAGFTIHGQIKGLPEKSAVFITD
                     ANKPTDTLARTTVKNGVFILKGQVTEPNLYEVNFNTAKKKTILFIGNENIKMEGAVDD
                     LSLLKVTGSSSQDDFMAFQREFNPYFASLNSLSKLANSPEGAPRRDSIAGAYSSISGV
                     IESKIDSFIQQRKSSFVSPFILVVTNQLSGDPLLLERRYQTLSPAVQGSMYGKYLNEQ
                     IETSKIGAVGSEAIDFTQNDTAGNPVSLSSYKGKYVLLDFWASWCQPCRMENPNVLAA
                     YGKFKSKNFTILGVSLDKTRDSWLRAIHDDKLAWVQVSDLKFWNNDAAAKYRIQQIPQ
                     NFLIDPNGKIVGKNLRGPELDERLCELLGCN"
     gene            complement(3805..4425)
                     /gene="hisIE"
                     /locus_tag="VGM24_05705"
     CDS             complement(3805..4425)
                     /gene="hisIE"
                     /locus_tag="VGM24_05705"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEY4154896.1"
                     /translation="MEVNFSKYADGLVPAIVQDDQTRKVLMLGFMNRQALDKTRELGK
                     LTFYSRSRKRLWTKGEESGHFLTLRNIAVDCDQDTLLIQATPEGPVCHTGADTCWDEK
                     NEADGFLPELEAIIQQRKNGRDENSYVAGLFKKGINKIAQKMGEESVELIIEAKDQDD
                     SLFLNEAADLLFHYLILLQAKGYNLQHVSDVLKQRHEKKLKSKNHT"
     gene            complement(4435..5193)
                     /gene="hisF"
                     /locus_tag="VGM24_05710"
     CDS             complement(4435..5193)
                     /gene="hisF"
                     /locus_tag="VGM24_05710"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY4154897.1"
                     /translation="MLTKRIIPCLDIQNGRTVKGTNFLNLRDAGDPVELGALYARQGA
                     DELVFLDITATHEKRKTLSALARRIAQQINIPFTVGGGISSVADVQVLLENGADKISV
                     NTAAFKNPGLIAELAKEFGSQCVVLAIDAKKAAGGKNYVHLNGGRVPTETTCIDWARK
                     GVDLGAGEILLTSMDQDGTKSGFALELTRMLARTLPVPVIASGGGGTLRHFAEVFKEG
                     FADAALAASIFHFGEIPIPELKKYLNTEGISIRI"
     gene            complement(5187..5906)
                     /gene="hisA"
                     /locus_tag="VGM24_05715"
     CDS             complement(5187..5906)
                     /gene="hisA"
                     /locus_tag="VGM24_05715"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY4154898.1"
                     /translation="MPIEVIPAIDIINGKCVRLTQGDYSRQKIYNEYPLEVALQFEDA
                     GLKRLHLVDLDGARQGAVKNWKVLETLAGKTNLVIDFGGGIAGSGDLQIVFDSGAAFA
                     TVGSIAVKNEKLFMEWLLRFGAKKFLLGADVREEKISISGWQEDTGIGIYDFIEKYIQ
                     AGIIQLFCTDIRLDGAMQGPSLELYKKIVLRFPDLHFIASGGIRSLDDISRLEEAGCR
                     GAIVGKAIYEGLIKLKDLARC"
     gene            complement(5920..6513)
                     /gene="hisH"
                     /locus_tag="VGM24_05720"
     CDS             complement(5920..6513)
                     /gene="hisH"
                     /locus_tag="VGM24_05720"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY4154899.1"
                     /translation="MNLVIIKYNAGNIRSVLYALERIGVEALVTDDPEIIRSADRVIF
                     PGVGEASTAMQYLRERKLDLLIRNLKQPVLGICLGLQLMCRNSEENETECLGIFEEEV
                     KRFNPGEEPQLKVPQIGWNNIYDLKTPLFREVPEGSFCYLVHGYYAGLGPNTIAKTDY
                     VQPYSSGLHKNNFYGVQFHPEKSAVTGELILKNFIQL"
     gene            complement(6510..7283)
                     /gene="trpA"
                     /locus_tag="VGM24_05725"
     CDS             complement(6510..7283)
                     /gene="trpA"
                     /locus_tag="VGM24_05725"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY4154900.1"
                     /translation="MSRIQELFQKKNRGILSVYCTAGFPALNSTVPVMKALEQSGADL
                     IELGMPYSDPLADGPVIQASSTVALSNGMTIAGLFQQLKEFRKEIRLPVLLMGYMNPV
                     LQYGFERFCRDAAAAGIDGLILPDLPMFEFEKEYGPLIRKYQLDFVFLVTPETAEERI
                     RKLDALSSGFLYAVSSSSTTGKNKDLSLQSAYFDRLQTMQLRNPVLIGFGIRDKESFS
                     KACRYAGGGIIGTAFIHALEGAGPEEIEPRTRNFLNTLT"
     gene            complement(7299..8501)
                     /gene="trpB"
                     /locus_tag="VGM24_05730"
     CDS             complement(7299..8501)
                     /gene="trpB"
                     /locus_tag="VGM24_05730"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY4154901.1"
                     /translation="MTDTITYQRPSEQGYFGDFGGAFIPEMLYRNVEELRTRYLEIMQ
                     EPGFRQEMDSLLKDYVGRPTPLYFADRLSERYGTRLFLKREDLCHTGAHKINNTIGQI
                     LLAERLGKKRIIAETGAGQHGVATAAVCALKGVECVVYMGEKDIRRQAPNVARMKMLG
                     ATVIPAVSGSKTLKDATNEAIRDWINHPADTHYIIGSVVGPHPYPDMVARFQSVISKE
                     VRSQLEEKTGRNYPDAVIACVGGGSNAAGAFYHFLDNKEVRLIAVEAAGLGIDSGMSA
                     ATTRLGKKGVLHGSCSLVMQTEDGQVVEPHSISAGLDYPGIGPLHANLFVSRRAEFLS
                     ATDQEALNAAFLLTRLEGIIPALESAHALAALEKIRFPKEASVVICLSGRGDKDMDTY
                     MKAMEEEA"
     gene            complement(8551..9195)
                     /locus_tag="VGM24_05735"
     CDS             complement(8551..9195)
                     /locus_tag="VGM24_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY4154902.1"
                     /translation="MRVKVCGMTQLDQVRTLDELGVDFAGFIFYPKSPRFAGKHIRGE
                     ELRKAKLRIGKVGVFVNTPYEEMMRTVDEYGLDMVQLHGDESPHRCEQIANYVSVIKV
                     FRLGEDDPISWIIKPYEESCDMFLFDTLGVGYGGTGKKFNWDLLKPAGIDKLFFLSGG
                     IEPEDVERLKAFSGEAVGKRLFAVDINSRFEISSGVKDMRKVEGFLGEMGSGKG"
     gene            complement(9140..9991)
                     /gene="trpC"
                     /locus_tag="VGM24_05740"
     CDS             complement(9140..9991)
                     /gene="trpC"
                     /locus_tag="VGM24_05740"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY4154903.1"
                     /translation="MGERNILEGIIAYKKEEVRQRKSEAPVKELEKSRHFSRLVFSLK
                     KALNDPAKTGIIAEFKRRSPSKGIIHPDASVERVTAAYAQYGASAISVLTDTVSFGGS
                     AEDLMIARLNPLPILRKDFTIDEYQITEARAMGADAILLIAACLSPAQTERLAAFAKA
                     SGLETLLEIHQESELTHLCDAVDVIGINNRDLKTFTVDINRSIELLKHLPAGKPVIAE
                     SGIRDAETICTLKSAGFNGFLIGEQFMKESDPAIAFASFVDQLKRKVYESKGLRHDAA
                     RPGENPG"
     gene            complement(10026..>10384)
                     /locus_tag="VGM24_05745"
     CDS             complement(10026..>10384)
                     /locus_tag="VGM24_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY4154904.1"
                     /translation="FAIIHSLDGYDELSLTGDARVITNDGEQVMTPEQLGKRRVSPSD
                     IYGGNTPEESAKIFQRILQGKGTWAQNAVVFANAAMALYCTGAYPDYNKAFEASVESL
                     ESGKALAALQQLLALQ"
BASE COUNT         2423 a         2834 c         2383 g         2744 t
ORIGIN      
        1 gacccttatg aaatctgcaa tcaaaaatct gctggaaatg cttaaggagc ttattccgga
       61 agccaatgcc ggccgctcgg aatataacaa tctgcgtctt atcccggttc cggttaaaaa
      121 ccaggatcag aaaagaccta atccatataa tccgaacaga taatgcttta tttaaaagaa
      181 gagcaaacgc ctaaaccagt tactccaatg gaaatctcat cccatcccga aaaattctcc
      241 aatgatatta ttgtggagat caggactgaa tcggaagacc attgcatcat tgtgctgact
      301 aatcattccg ggcgaattcg ccgaatgatg ggggttaatc tcaaccaggg acaaaatgat
      361 atccatgtag aaaatgtgga tacgctggaa gccgggctat atcagctcag cgtaaaaaat
      421 acggactcca ctcttttgta tacatctatt ctaaccaaat cataatatac catacctaaa
      481 cttaccaagt tttttttagc tgcactttta taaggattac gagggggcct ttccaggctc
      541 cttccttttt ttatgaaccc gataaaatac ggaaacctta ctttaaactc tgataaacca
      601 attgctcaaa tttcgttgag agatccccat gctggttggg ggtctgctgg gtcatgatca
      661 ggcaaaccag ttttgctttg ggatcggccc agtaggtcgt tccgaaaaaa cctccccagg
      721 agaatgtgcc gatatttcgc ggaccccggg ctgcggattt ttcaccggtt atttcaaatc
      781 ccagtccgaa attatcgatc ccatcgaagg gatgatccaa ctggccgctg gtcatcatct
      841 ccacggtccg gggcgccagt acctgcactc cgttgtattt ccctttattc aataacatct
      901 gcagaaatac cgcatagtcg tatgcggtgg aagtcaagcc ggctccgccc gagaaataat
      961 gctttttcac gattgggtaa tccggatcga tgcccgtttt gtttttatcc cagagcagca
     1021 cgtgacccag tgagtcttcc gtatagaccg ttgtcaggcg gtccgccttg gtggccggta
     1081 cattgaaata ggtatccttc ataccgaggg gtttgaatat tttctccgcc aggtagtctt
     1141 ccaggtcctt tccgctgatc tgttcaacca ggcagcccag cacatccacg cttaatccat
     1201 aagtccattt ctcaccggga tcatgcacca gcggaagttt cgccagccgc ttaacggttt
     1261 ccagcagatc catattctcc acacccagtc cgctgggaat cccggccttg gcatatatcg
     1321 ctttcatgga agggctgccg atacccgggt aatccagccc ggaagtatgc gtaaacagat
     1381 cccggaaagt tatttcccgc ttagccggtc tgctggtata gctgctgtcc ttttcattaa
     1441 aatgatccaa tacctgcgga tgcctgaatt ccggaataaa atcggcaatg ggctcgtcca
     1501 gcataaactt cccttcttca tataaagtca tggcccccac actgaccact gcttttgttt
     1561 gcgatgcgat ccggaacagg gcattcggcg gcaggggttt tttcgctgca ttgtctaaaa
     1621 atccatatcc tttaaattga ataagatggt tatcctgtac gatgatggtc gccaccccct
     1681 ttacccaacc cttctgaaca tatccgttga ccagggtgtc gatccggcgg agccgcgcat
     1741 aatcaatatc ccggctaacc gcctgcccat ttaaaataat ttgcccgtcg gcggaaaaat
     1801 ataagatggg cagaacaaag agaagggtaa aaatcttttt cattaaaatg ttttttaaaa
     1861 gatagtacta tttttctgaa atacgggagg cggcccggcc tggagctgcc ggatgcggga
     1921 agcataaacc ggtttgcttt aattccgcat gaattaactt tgtgtcatga tccggctttc
     1981 tgtaaatatc aacaagttcg ctaccctccg gaacagccgg ggcggaaata atcccgatat
     2041 cgtgaaagcg gccgtcgatg cccagcgctt cggggcggac ggggtgacag ttcatccccg
     2101 gccggacgaa cggcatatcc gttacgcgga tgtatttgcg atcaaaaaga gcatcagtac
     2161 agaattcaat attgagggga attgccagga acagaaattt atcgacctgg tgctgaaggc
     2221 aaaacccgat caggtgaccc tggtgccgga tgcgcttgac cagatcactt ccaaccatgg
     2281 ctgggacacc attcaggcgg cggacttcct cagggagatt atcccggttt tcaaacaggc
     2341 cggaatccgt gtttctattt ttgtagaccc ggttgaacgg atggtggaag gagccgccct
     2401 tacgggaacg gaccgggttg aattatatac ggaaggatac gccaaaacct ttccggagaa
     2461 taaggaaaaa gccattgcgc cctatctctc ggccgcaaaa aaggcaaaat cacttgggtt
     2521 gggtatcaat gcggggcatg accttgacct ggataacctg cactattttg ccaggtccat
     2581 tccgttcctg gacgaagtat ccattggcca tgcgctgatc tgcgacgcct tatacctggg
     2641 ctatgagaat gccgtgcaat tatataaacg ccagttgcag atcgactaag attaaaaggg
     2701 aaccccggga tcagttgcaa cccagcaact cacagaggcg ttcatccaat tcggggccgc
     2761 gtaaattttt cccgacgatc tttccattcg gatcaatcag gaaattctgc ggtatttgct
     2821 gtatccggta tttagcggcc gcatcgttat tccagaattt cagatcactt acctgaaccc
     2881 aggcgagttt atcatcatgg atcgctctta accaggaatc gcgtgtttta tccagggata
     2941 cccccaatat ggtgaagttc ttggatttga attttccata ggctgccagc acattcggat
     3001 tttccatgcg gcagggttgg caccagctgg cccaaaaatc cagcaataca tatttccctt
     3061 tgtaagaaga aagactgacc gggtttccag ccgtatcgtt ctgcgtaaaa tctatggctt
     3121 cggatccgac cgcgccgatc ttcgaggttt caatctgctc attcaaatat tttccataca
     3181 tgcttccctg gactgccgga gacagggttt gatatcttct ttccagcagg aggggatcac
     3241 cggacaactg gttggtcacg accaggataa aagggcttac gaaagaagat tttctttgct
     3301 ggataaaact gtcaattttg gattcaatca ccccggagat gctgctatag gctccggcta
     3361 tggagtctct cctgggcgcg ccttccggag aattggcgag tttgcttaaa gagttcaatg
     3421 aagcaaaata cggattgaat tcccgctgaa aagccataaa atcatcctga ctggaagatc
     3481 cggttacctt caataggctc agatcatcca cggctccttc catttttata ttttcattcc
     3541 cgatgaacag aatggttttc ttttttgctg tattaaagtt cacttcgtag agattcggct
     3601 ccgttacctg acccttaaga ataaataccc cattctttac ggttgtcctc gccagggtat
     3661 ccgtcggttt gttggcatcg gttataaata cggccgattt ctccggaagg cctttgatct
     3721 ggccgtgtat ggtaaaaccg gccggttttg cattctgggc cctggccaca ccagccagcg
     3781 cagttaagat aatcagcact cttttcatgt atgattttta ctttttaatt tcttctcgtg
     3841 ccgctgttta agaacgtcac ttacatgttg taaattatat ccctttgcct gtaataagat
     3901 caggtagtga aataacaggt ccgccgcttc gttcagaaac agggaatcgt cctggtcttt
     3961 ggcttcaatg atcagctcaa cactttcttc ccccattttc tgagcgatct tattgatccc
     4021 tttcttaaac aatccggcta cataagagtt ttcatcccgc ccgttttttc tttgctgaat
     4081 gatggcttcc agttccggca aaaatccgtc tgcttcattt ttctcatccc agcaggtatc
     4141 ggcacctgta tgacaaacag gtccttccgg agttgcctgg atcaggaggg tatcctgatc
     4201 gcaatcaacg gcgatgtttc tgagggtgag aaaatgacca ctctcttctc ctttggtcca
     4261 gagtcttttc ctggaacggc tgtaaaaagt cagtttaccg agctcccggg tcttatccag
     4321 ggcctgccgg ttcataaaac cgagcatcag cactttccgg gtctggtcat cctgaacaat
     4381 ggctggcacc agtccgtcgg catatttcga gaagttaact tccatataac aaaattagat
     4441 ccttatgctg attccttccg tattaagata tttttttaac tccggaatgg ggatttcacc
     4501 aaaatgaaaa atactggcag cgagggctgc atccgcaaag ccctctttga agacttcggc
     4561 aaaatggcgc agggttcctc cccctcccga tgcaatgacc ggaaccggca gggtccgggc
     4621 cagcatccgg gtaagctcca gggcaaatcc ggatttggtt ccatcctgat ccatggaggt
     4681 gagcagaatt tccccggcgc ccaggtccac acctttgcgc gcccagtcga tacaggttgt
     4741 ttccgtaggc acgcgtccgc cattcagatg cacgtaattt tttccgccgg ctgctttttt
     4801 tgcatcaatc gcaaggacca cgcactggct cccgaattct ttggccagct cggcaataag
     4861 tccggggttt ttaaaggctg ccgtattcac ggaaatttta tccgcaccgt tttccagcaa
     4921 cacctgcaca tccgccacgg aggagatgcc cccgccaacc gtaaagggaa tgttgatctg
     4981 ctgcgcgatc cggcgggcca gcgcactcag cgttttcctt ttttcatggg ttgccgtgat
     5041 atccaggaat accagttcat ccgcgccctg ccgggcatag agcgcaccca gctctacggg
     5101 atcccccgcg tcccgcaggt tgaggaagtt cgtgcctttt acggtgcgcc cgttctgaat
     5161 atccaggcag ggtatgattc ttttggtcag catctggcga ggtccttgag tttgattaat
     5221 ccttcataaa tcgctttgcc aacgatggcg ccgcggcagc cggcttcttc cagtctggat
     5281 atatcgtcca gggaacggat tccgccgctg gcgataaaat gcagatccgg aaatctcaaa
     5341 acaatctttt tatacaattc cagggaaggc ccctgcatgg ccccatcgag ccggatatcc
     5401 gtacaaaaga gttggattat tccggcctga atgtattttt ctataaaatc atagatcccg
     5461 atcccggtat cttcctgcca tccgctgatc gaaattttct cctcccgcac atccgctccg
     5521 agaaggaatt tcttcgcccc gaaccgcagc agccattcca taaaaagctt ttcatttttc
     5581 accgcaatgc ttcccacggt agcgaatgcg gctcccgaat caaatacgat ctgcaagtcc
     5641 ccgctgccgg cgatgcctcc gccaaaatca atgaccaggt tcgttttgcc agccagggtt
     5701 tccagcactt tccagttttt cacagccccc tgtctggccc cgtcaaggtc cacgaggtgc
     5761 agtcttttta aaccggcatc ctcaaattgc aaggccactt ccagcggata ttcgttatat
     5821 attttctggc gggaataatc gccctgggtc agtcgcacgc attttccatt tataatatca
     5881 atggctggaa tcacttcaat tggcataacc ggaagggagc taaagttgaa taaagttttt
     5941 taaaatgagt tcgccggtga ccgcactttt ctcgggatga aactgaaccc cgtaaaaatt
     6001 atttttatgc aggcctgaac tatagggttg tacgtaatct gtttttgcaa tcgtgttcgg
     6061 cccgaggccc gcataatatc cgtgcaccaa atagcagaag ctgccctccg gcacctcccg
     6121 aaaaagcggg gttttcagat cgtaaatatt gttccacccg atctggggca ccttgagctg
     6181 cggttcctct ccgggattga atcttttcac ctcttcttca aatataccga ggcactccgt
     6241 ctcattttct tcggagttcc tgcacatcag ctgcaggccc agacagatcc ccagcaccgg
     6301 ctgtttcagg ttccggatca gcagatccag tttccgttcc cgcaggtact gcatagccgt
     6361 gctcgcttct cccacgcccg gaaaaataac ccggtctgcc gaacggatga tttcgggatc
     6421 atcggtcacc agggcttcca cgccgatccg ttccagggca tacagtacag accggatatt
     6481 ccccgcatta tatttaatga tgaccaggtt cacgtgagag tatttaaaaa gttccttgtt
     6541 ctgggttcaa tttcttccgg cccggctcct tccagcgcgt ggataaatgc cgttccgatg
     6601 atgccgcccc cggcataccg gcaggctttg gaaaaacttt ctttatcccg gatgccaaac
     6661 ccgatcagca ccggattccg aagctgcatg gtttgcaacc ggtcgaaata agccgactga
     6721 agggacaggt ccttgttctt tccggtggtg gaagaggaag aaacggcgta caaaaagcct
     6781 gagcttaggg catcgagttt ccggatccgt tcttcagcgg tttccggggt tacgagaaaa
     6841 acaaaatcaa gctggtattt ccggatcagc gggccatact ctttttcaaa ctcaaacatg
     6901 ggcaggtcgg gaaggatcag cccgtcaatt cccgcagctg ccgcatcccg gcaaaatctt
     6961 tcaaacccgt attgcaaaac cggattcata tagcccatca gcaggacggg cagccggatt
     7021 tccttccgga attcttttaa ctgttgaaag agacccgcga tcgtcattcc gttggacagt
     7081 gccaccgtgc tgcttgcctg gatcacgggg ccgtcggcca gcggatcact gtacggcatc
     7141 cccaattcga tcagatcggc gcccgactgc tcgagcgctt tcatcaccgg aaccgtactg
     7201 ttcaatgcag gaaaacccgc cgtgcagtaa acgcttagga tcccccggtt ttttttctga
     7261 aaaagttcct gaatacgact catagagaat gataaaaatt atgcttcttc ttccattgct
     7321 ttcatatagg tgtccatgtc cttgtcccct cggccgctca ggcagataac gacggatgcc
     7381 tctttcggaa accggatttt ttccagggcc gccagggcgt gggccgattc cagtgcagga
     7441 ataatccctt ccagcctggt taacagaaaa gcagcattca gtgcttcctg atccgttgcg
     7501 ctgaggaact ctgctctccg ggaaacgaat aaattcgcgt gtaacggtcc gatgccggga
     7561 tagtcgagac ctgccgaaat actgtgcggt tccacaacct gtccgtcttc cgtctgcatg
     7621 accaggctgc agctcccgtg cagcacccct ttctttccca ggcgggtggt ggccgcactc
     7681 attccgctgt ctattcccag cccggcagct tccacagcga tcagtcttac ctctttgttg
     7741 tcgaggaaat gataaaacgc gccggcggca ttactgcctc ccccgacgca ggcgatcacc
     7801 gcatccggat aattgcggcc ggttttctcc tccagctgcg atctgacctc tttgctgatc
     7861 acgctctgaa agcgggccac catatccgga tagggatgcg gacccaccac gcttccgata
     7921 atataatgcg tatcggccgg atgattgatc cagtcccgga tcgcttcatt cgtcgcatct
     7981 ttcagtgtct tacttccgct gaccgccggt attacggttg cgcccagcat tttcatgcgg
     8041 gccacattcg gcgcctgccg gcggatgtcc ttttctccca tgtacacaac acattccact
     8101 cccttcaggg cacagacggc cgcggtggcc acaccgtgct gccctgcccc ggtttccgca
     8161 ataatccttt ttttaccgag tctttcggcc agcaggatct gtccgatggt attattgatt
     8221 ttatgggcgc cggtgtggca aagatcttcg cgtttcagaa acaggcgcgt cccgtaccgc
     8281 tcacttaatc tgtcagcaaa atacaagggg gtcgggcggc ccacataatc tttcagcagg
     8341 gaatccatct cctgccggaa tccaggctcc tgcatgatct ccaggtagcg ggtcctcagc
     8401 tcctccacat tccggtaaag catttccggt ataaaagctc ctccgaaatc gccgaaatac
     8461 ccctgctcgg aaggccgttg ataagttatt gtatcagtca tattattccg ctttctattg
     8521 tgaaggcatt tatttttccg caacgctaaa ctatcctttt ccggaaccca tctctcccag
     8581 aaaaccttcc acctttcgca tatcctttac gccgctgctt atttcaaaac ggctgttgat
     8641 gtccacggca aaaagccttt tacccaccgc ctccccgctg aaggctttca atctttccac
     8701 atcttccggc tcgataccgc cgctcaggaa aaataattta tctatccctg ccggtttcaa
     8761 cagatcccag ttgaattttt ttcctgttcc cccatagccc acccccaggg tatcaaacag
     8821 gaacatgtcg caggattcct cgtaaggttt gataatccac gatatcggat catcctcgcc
     8881 gagacggaac accttgatca cggacacata atttgcaatt tgttcgcacc ggtgcgggct
     8941 ttcatctcca tgcaactgta ccatgtccag cccgtattca tccaccgtac gcatcatctc
     9001 ctcatagggc gtgttgacga atacccccac tttcccgatc ctcaattttg ccttccgcaa
     9061 ttcctctccg cggatatgct tccccgcaaa acgcggcgat ttcgggtaaa agataaaccc
     9121 ggcaaaatca acgcccagtt catccagggt tctcacctgg tcgagctgcg tcatgccgca
     9181 aacctttact ctcataaacc ttccgtttta attggtcaac aaaggaggca aaagcgatcg
     9241 ccggatcgga ttctttcata aactgttctc cgatcagaaa gccgttgaag ccggccgatt
     9301 tcaaagtaca gatcgtttcc gcatcgcgga taccgctttc ggcaataacc ggtttgccgg
     9361 caggtaaatg tttcaacagt tcaatcgaac ggttgatatc cacggtgaat gttttcaggt
     9421 cgcggttatt gataccgatc acatcgacgg catcgcaaag atgcgtcaat tcggattcct
     9481 gatggatctc cagcagcgtt tccagcccgg atgctttcgc gaaagccgca agcctttccg
     9541 tttgtgccgg gctcaggcag gccgcaatca gcagaatggc atcggctccc atggcgcgcg
     9601 cttcggttat ctggtattca tcgatcgtga aatcctttct gagtatcggc aacgggttca
     9661 ggcgggctat catcaggtct tctgcagatc ccccaaagga aacggtgtcg gtcagcaccg
     9721 agatcgcgga agcgccgtat tgcgcatagg cggcggttac cctttccacc gaggcatcgg
     9781 gatgaatgat cccttttgaa ggagagcgtc ttttaaattc tgcgatgatc ccggtttttg
     9841 ccgggtcatt cagtgctttt ttgagcgaaa agaccagcct tgaaaaatgg cggctttttt
     9901 ccaattcctt taccggggct tccgatttac gctgccgcac ttcctccttt ttataggcaa
     9961 taattccttc gagtatattt cgttcaccca tttttaaaat ttcagaatat tcaaattcct
    10021 ctttttcatt gcaacgccag caattgctgc agggcggcca gcgcttttcc gctctccagg
    10081 ctttccaccg aggcttcaaa ggccttgttg taatccgggt atgccccggt gcaatataat
    10141 gccatggccg cgttggcgaa taccaccgca ttttgggccc aggtcccttt tccctgcaga
    10201 atccgctgaa aaattttggc tgattcctcg ggtgtgttgc cgccatagat atcggatggc
    10261 gatacccttc tttttcccaa ctgttccggc gtcatcacct gttctccgtc attggtgatc
    10321 acccgcgcat ctccggtgag ggataattcg tcatatccgt ccaggctgtg tataatggca
    10381 aaag
//
LOCUS       DASYCU010000287         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4268267, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000287 DASYCU010000000
VERSION     DASYCU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4268267"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VGM24_05750"
     CDS             <1..594
                     /locus_tag="VGM24_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4154905.1"
                     /translation="KFLKQITRDEDAQPKIIVEGKQNVSIAALKDELTGAMRSIHKLS
                     PGQEDDYALNDIQSLTKQTEVIFSNLNKGGWAIAALSLIVGMFGVANIMFVTVRERTG
                     QIGLKKALGAKKSTILSEFLLESSFLCIIGGLIGLLAVFILAAVFSSILSFKVFIPIN
                     IIGLAVSICLFVGVVAGIIPAMTAAKMDPVVAIRSTT"
     gene            650..1669
                     /gene="tsaD"
                     /locus_tag="VGM24_05755"
     CDS             650..1669
                     /gene="tsaD"
                     /locus_tag="VGM24_05755"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY4154906.1"
                     /translation="MPVTLLAIESSCDETAAAVCRDGRILSNIVASQQIHSLYGGVVP
                     ELASRAHVRHIVPVVDQAMKTAGSDLSSLQAIAFTRAPGLIGSLLVGSQFAKSLSLSL
                     NIPLIGIHHMQAHVLANLIEGATDQQALPAFPFLCLTVSGGHTQIVICYSPSELKVIG
                     ETLDDAAGEAFDKSAKMLGLPYPGGPLIDRYAASGNPARFKFPEPRIPGLNFSFSGLK
                     TAILYFLQEGREKDPGFVDQNLPDICASIQSRIISILLNKLKKAAAETGIKTVCLAGG
                     VAANKGLRKTFEQTGSELGWKTFIPKMEYCTDNAAMIAIAGYYQYLDQAFSPLSVTVS
                     ARTSW"
     gene            1680..2390
                     /locus_tag="VGM24_05760"
     CDS             1680..2390
                     /locus_tag="VGM24_05760"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY4154907.1"
                     /translation="MPNSYFRFKQFTIQQDRCSMKVCTDSCILGAWTASRLKRSLRVL
                     DIGAGTGLLTLMLAQKNQCRFDAIELDRPTFEQAAENIRDSPWADRIGLFQGDVRNYP
                     FLHSYDFIISNPPFFESGLPSPFPEINKARRAESLPPEELISVIRRLLNAGGAFSLLL
                     PFYRTAYVEKLAAEKGFFLQEKLLMRQTPAHGPFRSALLFSDSKPISAAVQELALRSG
                     NGVETDELLALLQDYYLR"
     gene            complement(2433..3641)
                     /locus_tag="VGM24_05765"
     CDS             complement(2433..3641)
                     /locus_tag="VGM24_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4154908.1"
                     /translation="MIQIGLIRETKIPADNRVALIPSQCKWLQKNFPELKILLQPSAN
                     RCFTDKEYRMAGVEVTEDLGTCDFLFGIKEVKTDQLIPGKKYLFFSHTRKMQAHNRAL
                     FRAILDKKITLIDYECMEHDDGQRILGFGFFAGVVGAHNGIMAYGQRTGKYHLPRVYQ
                     QSSFRQLIHSYFGLRLPNIKVAITGSGRVAHGLLEIMNLLGVIEVEPEEYLSRDFSYP
                     VFTQLKGHALYSHKQNGSYNREDFHRNPAKYNCIFKPYSKTTDILLNGIFWDSRMPRL
                     FEWEDLQSPEFRIRTIADITDDRDGSVPCNLGDSNMENPVYGVDKISRQKTAPFLPGS
                     IDLMAFGNLPNELPRDASRYFGEQLIKFVLEDLLKNGSPVIERATMVDRGKITKGFDY
                     LKEYGGQGDD"
     gene            4045..>4744
                     /locus_tag="VGM24_05770"
     CDS             4045..>4744
                     /locus_tag="VGM24_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4154909.1"
                     /translation="MKNISQHSKSGICRTAFLFLVILMTANRLPAQNRPVTGRVLANE
                     SDSLIAGATVKVRGATTTVFTNADGTFSINAAPNATLIVSTIGYTTQEVPLNNRSSVD
                     IRLISEAMSLQQIVVVGYGTQKRKDLTGAISSVSADQIAKVPVTTLDQALQGRSSGVQ
                     VTNNDGAPGGGVQVQIRGIGSLGTNDPLYVVDGYPLSGGINTLNPNDIASIDILKDAS
                     ATAIYGNRASNGVVI"
BASE COUNT         1155 a         1227 c         1179 g         1183 t
ORIGIN      
        1 aaatttttaa aacagataac ccgcgatgag gacgcacagc ccaagatcat cgtggaagga
       61 aaacagaatg taagcattgc cgccttaaaa gatgagttga cgggggccat gcggtccatt
      121 cacaaactga gcccgggtca ggaggacgat tacgcgctga atgatatcca gtccctcaca
      181 aagcagacag aggtcatttt ttccaacctc aacaagggcg gctgggccat tgcggcgcta
      241 tcccttattg tagggatgtt cggcgtggcc aatatcatgt ttgttacggt gcgcgaaaga
      301 actggccaga tcggtttgaa aaaagccctg ggggccaaga aaagcaccat cctttctgaa
      361 ttcctgcttg aatcttcttt tctgtgtatt atcgggggcc tgatcggtct gctcgccgta
      421 ttcattctgg cggctgtttt ctcctccatt cttagtttta aggtttttat cccgatcaat
      481 atcatcggac tggcggtgag tatctgcctc tttgtaggcg ttgtggcagg catcatcccg
      541 gccatgacgg ccgctaaaat ggatccggtg gttgccatcc ggtccactac ctgaagactt
      601 tctggtttcc ccgttcgcgg atatctttgc cggaataaaa atcatccatt tgcctgttac
      661 tttattagcc attgagtctt cctgtgatga aacggcggcc gcggtttgtc gcgatggccg
      721 tatcctttcc aatattgttg cgagccagca gattcacagt ctgtatggcg gggttgttcc
      781 cgaactggcc agccgggcac atgtccggca catcgttccc gtcgtggatc aggcaatgaa
      841 aactgccgga tcggatcttt cctccctgca ggcaatcgct ttcaccaggg cgcccgggtt
      901 gatcggctcc ctgctcgtcg gttcccagtt tgccaaatcg ctttctcttt cattaaatat
      961 tcctttgatc ggcatacatc acatgcaggc gcatgtgctg gcgaatctga ttgaaggggc
     1021 aacagaccag caggccttgc ctgcatttcc ctttttgtgt ttaaccgtga gcggaggcca
     1081 tacgcagatt gtcatttgct attcaccgtc cgaattgaaa gtgatcgggg agaccctgga
     1141 tgatgccgcc ggagaagcct ttgacaagtc ggcgaaaatg ctgggacttc cctatcccgg
     1201 cggaccgttg atcgaccggt atgcggcttc cggaaacccg gcccggttta aattcccgga
     1261 accgaggatc cccggattga atttcagttt cagcgggctc aaaaccgcga tcctgtattt
     1321 tctgcaggaa ggccgggaaa aagatccggg attcgtcgat caaaatcttc cggatatctg
     1381 cgcctccatt cagtcgcgca tcatttccat tctgctcaac aaattaaaaa aagcggcggc
     1441 ggaaacaggt atcaaaaccg tttgcctggc gggcggcgtt gcggccaaca agggcctccg
     1501 gaagactttt gagcaaacgg gcagcgaact tggatggaag acattcatac ccaaaatgga
     1561 atattgtacc gacaatgccg cgatgattgc catcgccgga tattaccaat acctcgatca
     1621 ggccttttcg ccactaagcg tgaccgttag tgccaggact tcctggtaaa cctgccgata
     1681 tgcccaatag ctatttccgc tttaagcaat ttaccattca gcaggaccgc tgcagcatga
     1741 aagtatgtac cgactcgtgc atactggggg cctggacggc aagccggtta aagagatccc
     1801 tgcgggtgct ggatatcggt gcggggaccg ggttgctgac cctgatgctg gcccagaaaa
     1861 accagtgtcg cttcgatgcg attgaactgg acaggccgac tttcgaacag gctgccgaaa
     1921 atatcaggga tagtccctgg gcggaccgca tcggcctctt ccagggggat gtgcgcaact
     1981 atccattcct tcattcctac gatttcatca tcagcaatcc gccctttttt gaatcggggc
     2041 tcccctctcc tttcccggaa ataaataaag cgagacgtgc ggagtcctta cctcccgaag
     2101 aactgatttc ggtgatccgg cgattgctga atgccggcgg agcgttcagc ctcctgcttc
     2161 ccttttaccg tacggcatac gttgaaaaac ttgctgcaga aaaaggattt ttcctgcagg
     2221 aaaaactact gatgcgccag accccggcgc acgggccctt ccggtcggca ttattatttt
     2281 ctgattcaaa acccatatcc gctgccgttc aggaactggc tctccgcagc gggaacggag
     2341 tggaaacaga tgaattgctt gcgttactgc aggactatta tttacgttaa acaagagatc
     2401 ttctccgcct ccctcattct ctccaaaagg tctcaatcgt ccccctgtcc gccgtattct
     2461 ttcaaatagt caaaaccctt cgtgatcttt ccccggtcaa ccatggtagc tctttcaata
     2521 accggagacc cgtttttgag caggtcctcc agtacaaact tgatcagttg ctccccgaaa
     2581 tagcgggacg catcccgggg cagttcgttg ggaagatttc cgaaagccat cagatcaatg
     2641 gatccgggca gaaagggcgc ggtcttctgc cgggatattt tatccactcc gtaaaccgga
     2701 ttttccatat tcgaatctcc cagattgcag ggaacggaac cgtcccggtc gtccgtaata
     2761 tctgcgatag tccggatgcg gaattcaggc gattgcagat cttcccattc aaacagtctc
     2821 ggcatacgcg aatcccagaa aataccattc agcagaatgt ccgtcgtttt cgaatacggt
     2881 ttgaaaatac aattatactt cgcgggattg cggtggaagt cctcccggtt atacgatccg
     2941 ttttgtttat ggctatacaa tgcatgtccc ttgagttggg taaaaacagg ataagaaaaa
     3001 tctctggaaa ggtattcctc cggttccact tcaataactc cgagcaggtt catgatctcc
     3061 agcaggccgt gggccacccg gccggatccc gtaatggcga cttttatatt gggaagccgg
     3121 aggccgaagt aggaatggat gagctgacgg aaactggatt gctggtagac cctgggcaaa
     3181 tgatattttc cggtacgctg gccgtaagcc attatcccat tatgcgcacc gaccacaccg
     3241 gcaaaaaatc caaatcccag gatccgctgc ccatcatcgt gttccatgca ttcataatcg
     3301 atcagggtga tttttttatc gaggatcgcg cggaacaggg cgcggttatg ggcttgcatt
     3361 ttgcgggtat gagagaaaaa cagatatttt tttcccggaa tgagctgatc tgtttttact
     3421 tccttgatcc caaataaaaa atcacaggtt ccgaggtctt ccgtcacttc cacgcccgcc
     3481 atccggtatt ccttatcggt gaaacaacgg tttgcagaag gctggagcag gatcttgagc
     3541 tccggaaaat ttttctggag ccatttacat tgggagggga tcagggcaac gcggttgtct
     3601 gccggaattt tagtctcccg gattaatccg atttgaatca ttaaaggcat tgtttgatcc
     3661 ggcaagttaa ttatgttccc gttacggccg gaagatgcat aagattccgg ctaaaaaagg
     3721 aatttcttac cgttgagatt cgccggaata ccccccgtag ttttttgcat tttccgaatc
     3781 ctttttcatg aaaatcccgg ctttaatcaa ataacagcaa ggaatggcaa aaataatacc
     3841 aataaaatga taaaaataca ctcaaatttg aataggcaaa ttcacgaccg ggtatttgcg
     3901 aatattattc tttaaaacca aaacggaaca tcatgcttgt cctttcacct gcccaaaggc
     3961 gactccctcc tttatttttt cgaagcttca ttaccggccc tttccatcaa acccacaaat
     4021 accacccagt taaacattca taatatgaag aacatctcac aacattccaa gagcggcatc
     4081 tgccgcaccg ccttcctgtt cctggtgatc ttaatgacag cgaatcgcct gccggcgcag
     4141 aacaggccgg tgaccggaag ggtgctggca aacgaaagcg atagtttgat tgcgggggca
     4201 accgtgaaag tgaggggtgc aacgaccacg gtattcacca atgcagacgg aaccttcagc
     4261 atcaatgccg ctccaaatgc gacgctgatt gtcagcacga ttgggtacac tacccaggaa
     4321 gttccgctga ataaccgcag ctcggtcgat atccggctca tttcggaagc gatgtccctg
     4381 cagcagatcg tggtggttgg ttacggcacc caaaagcgca aggatttaac cggagccatc
     4441 agcagcgtat cggcagacca gatcgcaaag gttccggtca ctacgctgga ccaggcgctc
     4501 cagggaaggt cttccggcgt tcaggttacg aataatgacg gcgcaccggg cggtggggtc
     4561 caggttcaga taagaggtat cggaagcctg ggaacgaatg atcccctgta tgtggtggat
     4621 ggttatccgc tgagtggtgg aataaacacg cttaacccca acgatattgc ttccattgat
     4681 atcctcaaag acgcctctgc cacggcgatt tacggaaaca gggcgtccaa tggcgtggtt
     4741 atta
//
LOCUS       DASYCU010000288         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1077823, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000288 DASYCU010000000
VERSION     DASYCU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077823"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VGM24_05775"
     CDS             complement(<1..553)
                     /locus_tag="VGM24_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4154910.1"
                     /translation="MPNAAHVHRNQLFTASCLALLVTSLSFGIRAGILNSLGVQFQLN
                     ASELGTITATAFWGFPLAIIIGGFIVDIIGMKKLLVLAFIFHLLGIVLTIFAGGYWTL
                     FFSTLLIGIANGTVEAACNPLVASLFPENKTTKLNHFHLWFPAGIVIGTLVVFLLNKV
                     GVSWQVEVATMLIPTLIYGYLFSK"
     gene            719..1882
                     /locus_tag="VGM24_05780"
     CDS             719..1882
                     /locus_tag="VGM24_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4154911.1"
                     /translation="MNRKLRMGMVGGGKDAFIGAIHRIASHMDGLIELSCGALSIHPD
                     VAKLSGEQLFLPPDRSYLNFEEMLEKESKIPADRRIDFVTIVTPNFAHFAPAMMALDK
                     GFNVVVEKPITFTLDEAKQLKKKVEETGLVLCLTHTYSGYPMVKQARAMVQQGVFGKL
                     RKIWVEYPQGWLSRKTEREGNVQAAWRTDPKKSGKSGCMGDIGTHAAHLAEYISGLRI
                     TRICADLNIFVEGRALDDDGNVLLKFDNGAAGVLMASQVAAGEENALKIRIYGEKGGL
                     EWSQQEPNSLIIKWLDQPTQILRAGVGYKEPLSSFATHNLRTPGGHPEGYLEAFGNIY
                     RNFAQTLTARIDGTTPSAESLDFPGVEDGIRGMEFIDHVVASAASSEKWYDCK"
     gene            1907..2962
                     /locus_tag="VGM24_05785"
     CDS             1907..2962
                     /locus_tag="VGM24_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY4154912.1"
                     /translation="MKTIKGPGIFLAQFMGDAEPFNSLASICKWAASLGYKGVQIPTW
                     DARCIDLKKAAESKTYSDEIKGTVTAAGLEITELSTHLQGQLVAVNPAYDALFDGFAP
                     EAFRNNPKARQEWAVQQLHYAAKASKNLGLNAHATFSGGLLWPTVYPWPQRPAGLVDT
                     GFKELAKRWKPILNAFDENGVDLCYEIHPVEDLHDGVSYERFLKEVNDHPRACLLYDP
                     SHFVLQQLDYLSYIDYYHERIRMFHVKDAEFNPTGKQGVYGGYQNWIDRAGRFRSLGD
                     GQVNFKSIFSKLTEYDFAGWAVLEWECCIKHPEDGAREGAPFIKDHIIRVTEKAFDDF
                     ASTGSDESFNRAVLGIK"
     gene            3069..>3328
                     /locus_tag="VGM24_05790"
     CDS             3069..>3328
                     /locus_tag="VGM24_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4154913.1"
                     /translation="MKKAILVFLCSSVVIAACNSGGSSSKSADSVTTATPATPEATTP
                     SEKGLELIGASDCTTCHRLHKESAGANIGPAYSEVAAKYSPAA"
BASE COUNT          873 a          833 c          909 g          713 t
ORIGIN      
        1 gtttggaaaa aagatatccg tagatcaggg tcggaatcag catggtggcc acttccacct
       61 gccagctcac gcccacctta ttgagcagga agactaccag ggtgccgatg acgatcccgg
      121 cggggaacca gagatggaaa tgattcagtt tggtcgtttt attttccggg aatagagagg
      181 ccaccagggg attacaggct gcttccactg ttccgttggc gatgccgatc agcagggtgg
      241 aaaaaaacag ggtccagtat ccgccggcaa aaatggtgag cacgatgccc agcaggtgaa
      301 agatgaatgc cagcaccagc aattttttca tcccgataat atccacgata aaacccccaa
      361 tgatgatggc cagcggaaaa ccccagaatg ccgtagcggt aatggttccc agttcagacg
      421 cattcaactg aaactggacc ccgagactgt tgaggatccc ggcgcggatt ccgaaggaaa
      481 gggaagtgac cagcagggcc aggcaactgg cggtaaagag ctggttgcgg tgaacgtgag
      541 cggcattcgg catggcaatc ggtttaagtt aaatcgtcat taatacattt cttccaacag
      601 gcagtaaatg taatttttta tttcagaaaa taaaaaaatc aaacaaatcc gggttctgcc
      661 gcgtatttgt acattattat tctaaacttg cattgaaaat gatctaaaaa ccaaggttat
      721 gaatagaaaa ttacgaatgg gcatggtggg tggagggaaa gacgctttca tcggggccat
      781 ccatcgaatc gcctcccata tggacgggct catcgaactt tcctgcggcg cgctcagcat
      841 ccatccggat gtggcgaagc tttcgggcga gcagttgttt cttccccccg accgcagcta
      901 cctgaatttt gaggagatgc tggagaaaga gagtaagatc cctgccgacc gccggatcga
      961 tttcgttacc atcgtcacgc cgaattttgc acacttcgca ccggccatga tggcgcttga
     1021 taaaggattc aatgtggtgg tggaaaagcc cataaccttc accctggacg aggcaaagca
     1081 gttaaaaaag aaagtggaag aaaccgggct cgtgctctgt ctcacccaca cctattccgg
     1141 atatcccatg gtaaagcagg cccgggcaat ggtgcagcag ggcgtgttcg ggaagctgag
     1201 aaagatctgg gtggaatacc cgcagggatg gctcagccgg aaaacggaac gggaaggcaa
     1261 tgtacaggca gcctggagaa cggatccgaa gaaatccgga aagagcggct gtatgggaga
     1321 tatcgggact catgccgccc acctggcgga atatatcagc ggacttcgga tcaccaggat
     1381 ctgcgccgac ctgaatattt ttgtggaagg ccgcgcactg gatgacgatg gcaatgtatt
     1441 gttgaaattc gacaacgggg ccgccggcgt gctgatggcc tcccaggtgg ccgcggggga
     1501 agagaatgcg ctgaagatcc ggatctatgg tgaaaaaggc ggactggaat ggagccagca
     1561 ggaaccgaat tccctgatca taaaatggct ggatcagccg acccagatcc ttcgggcggg
     1621 cgtgggctat aaagaaccgc tatcctcttt tgccacgcat aacctgagga ctcccggcgg
     1681 gcatccggaa ggatacctgg aagccttcgg gaatatctac cgcaattttg cacagacctt
     1741 aaccgcccgg atcgacggca ccacgccgtc tgccgaaagc ctggatttcc cgggggtcga
     1801 ggatggcatt cgcggaatgg aattcattga tcacgtggtg gcttcggctg cgtccagtga
     1861 aaagtggtac gactgtaaat agcctcctca attcaaaata taaaaaatga aaacaataaa
     1921 ggggcccggc attttcctgg cgcagttcat gggagacgcc gagccgttca attcgctggc
     1981 atccatttgt aaatgggccg catctctggg atataagggc gtccagattc ctacctggga
     2041 cgcgcgctgc atcgatctga aaaaagccgc cgaaagcaaa acctattcgg acgagatcaa
     2101 agggaccgta acggcggccg gactggagat caccgaattg tccacgcacc tgcagggaca
     2161 gctcgttgcg gtgaacccgg cttacgatgc gctgtttgac ggatttgcac cagaagcttt
     2221 ccggaataat ccgaaagccc ggcaggaatg ggccgttcag caattgcatt atgccgcgaa
     2281 ggcttccaaa aacctcggac tgaatgcgca cgcgaccttc agcggcggcc tgctctggcc
     2341 cacagtctac ccctggcccc agcgccccgc cggtttggtg gataccggtt tcaaagaact
     2401 ggctaaacga tggaagccca tcctgaatgc gttcgatgaa aacggcgttg atctttgtta
     2461 tgaaattcat ccggtagaag atctgcatga cggcgtcagc tacgaacggt ttttgaaaga
     2521 agtgaacgat catccccgcg cctgtttatt atatgatccc tcccatttcg tattgcagca
     2581 actggactat ctcagctata ttgattatta ccacgagcgc atccgcatgt tccatgtaaa
     2641 agatgcggaa ttcaatccca ccggcaaaca gggcgtttac ggcggttacc agaactggat
     2701 cgaccgggcc ggaaggtttc gctcactcgg ggacgggcag gtgaatttta aatccatttt
     2761 cagtaagctg acggaatatg attttgccgg atgggcagtg ctggaatggg agtgctgcat
     2821 caaacatccg gaagacgggg ccagagaagg cgctccgttt atcaaagacc atataatccg
     2881 ggtcacagaa aaagcattcg atgattttgc ctcgacgggc tccgacgaat cgtttaaccg
     2941 cgcggtactc ggtataaaat aaaaatcggg atccgatgtg cagaaagtat ctccgccgtt
     3001 cttcatttcc ggcggggatt ggaatttttt cacgaaattt atgcccttac tctataattc
     3061 aagtaacaat gaaaaaagca attctagttt tcctgtgcag ttcggtggtg atcgcggcct
     3121 gcaattccgg aggctcttct tccaaatccg ctgactcagt cactaccgcg acgccggcaa
     3181 caccggaagc cacaacccct tctgaaaaag gactggagct gatcggggcc agtgattgca
     3241 ctacctgcca tcgcctgcat aaagaatctg ccggcgcaaa catcggacct gcttacagcg
     3301 aggtggccgc aaaatattca ccggctgc
//
LOCUS       DASYCU010000289         8018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1661428, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000289 DASYCU010000000
VERSION     DASYCU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661428"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VGM24_05795"
     CDS             <1..1008
                     /locus_tag="VGM24_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEY4154914.1"
                     /translation="LPGTDGKTYSLASFSGKEVLVIIFTCNHCPTAQAYEDRIIQLTR
                     DYSAKNVAVVAIMPNDPLSINLDELGYTDMGDSFEEMKIRAKEKKFNFPYLYDGDTEK
                     TARAYGPVATPHVFIFDKKRILRYSGRIDDMEKPTKTPTRYDTRNAIDALLAGKPVPV
                     TSTKVFGCSIKWAEKSDWIKDSRENWAKEPVRLDTLGEAGLRDLLKNHSDKLRLINVW
                     ATWCGPCVAEFPEFITMNRMYRQRDFEFISISADELAKEAKALQFLQKQQASGRNYIF
                     SGDNKYNLIEAIDPNWQGALPYTLLVEPGGKIVFAREGAIDPFELKKAIVDNKYIGRY
                     Y"
     gene            complement(1012..1821)
                     /locus_tag="VGM24_05800"
     CDS             complement(1012..1821)
                     /locus_tag="VGM24_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4154915.1"
                     /translation="MTKKINGLIAMIAVALFLGSCVPTRKFDASQRALQSARSDSARL
                     AARVSTLESTVSQLKGQVNDLTNKAGELSTDAANKASQLNKSQQELADQQKRLEQLQA
                     LMDQQKKATQEIRKKMSDALVGFNSNELTVSVKNGKVYVSLQENLLFPSGSAVVNPKG
                     KEALGKLAEVLNANPDITVDIEGHTDSIPIRGKYKDNWDLSTARANSIVRILTQDYSV
                     DPVRVISSGHSLYDPVQTNSTPEGRALNRRTEIILSPRLDELYKLLEGTSS"
     gene            complement(1977..3605)
                     /locus_tag="VGM24_05805"
     CDS             complement(1977..3605)
                     /locus_tag="VGM24_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582016.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HEY4154916.1"
                     /translation="MHKLQPADYVVFFIYFIVVSAYGYYVYHKKKFASTDSKDFFLAE
                     GSLTWWAIGASLIASNISAEHFIGMSGSGFALGLAISTYEWMAAATLIIVAVFILPIY
                     LKNKIFTMPQFLARRYNDQVSTMMAIFWLLVYVFVNLTSIIYLGALAITSISDISFGW
                     SVAGLSAFSIIVTLGGMKVIGYTDVIQVLVLLVGGLVTTYLALNLLSGHFGFGSNVWK
                     GLSILRKEAPDHFHMIFHKGNPYYKDLPGLSVLIGGMWINNLAYWGCNQYIIQRALGA
                     NLKTARSGILFAAFLKLLVPVIVVLPGITMFVLHKNGLFQHEMMQGGVIKPDHAYPTL
                     MNLLPAGLKGVAFAALTAAVVASLAGKANSISTIFSLDIYKKFFNKEASEKKLVGIGK
                     WVVIVAMGIAALIAPALKNLDQAYQFIQEYVGFISPGVLAIFLLGFFWKRTTAAAALT
                     GTILTIPISTVLKFLPSWTGGAFPDYPFLDRMSITFILIVIIMIFISLSKPAADQAAH
                     SILIDRKQFSVSPAFIVGSVFIIGILSALYTVFW"
     gene            complement(3652..4539)
                     /locus_tag="VGM24_05810"
     CDS             complement(3652..4539)
                     /locus_tag="VGM24_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HEY4154917.1"
                     /translation="MSSSKSLSQTEISPLASLDEWEEDVLKRYPEADKPAMAKQKEEY
                     RNYEAPARDTVKEFYRLNHTWQSYAFVQEKRAEFLQFNKREMPVWDAFQFLNQLVDDS
                     DPDTDLDQFQHLLQTAEAIRADGHPDWMVTVGLIHDMGKVLCLFGEPQWAVVGDTFPV
                     GCAHSEKIVYPEFFALNPDVKDEVLNSKLGLYSENCGLRNVSMSWGHDEYVYQMMKDS
                     LPEPALYMLRYHSFYAWHRESAYDYLLDEHDREMLKWVKLFNPYDLYSKNPTVPDWKE
                     LKPYYEDLVSKYLPATLKF"
     gene            4799..5620
                     /locus_tag="VGM24_05815"
     CDS             4799..5620
                     /locus_tag="VGM24_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4154918.1"
                     /translation="MIKTITAIFLFCAVSANAQQHKLVKLWETDSVVAIPESVLPDLK
                     NNILYVSLIDGGGWDADGKGGIARLGTDGKHYEPNWVTGMNAPKGLGKYGNRIYAADI
                     SEVVVVDISHRKIIKKIAIEGASGLNDITVSDKGIVYVSDSKTSRIWKIENDKPSLYL
                     ENVNGANGLKAIKNDLYYAEGPVVMKADAQKKLTRVAEVPQSIDGIEPVGNGDFIVTG
                     WSGYIFYVHANGAVDALLDSHLEKINTADLGYDPARRILYVPTFLAKKVIAYKLN"
     gene            5670..6128
                     /locus_tag="VGM24_05820"
     CDS             5670..6128
                     /locus_tag="VGM24_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY4154919.1"
                     /translation="MASFKSLSVFLFFTSLCVLAFSVTGFRQKTHTEPWTEDQLLAPE
                     DLARTINDPNAKQPVILCVGPGAMIKGSLDMGPAHEKAGYEKLKDQLEKLPKNADIVI
                     YCGCCPFDHCPNIRPAFALLNEMKFNNQRLLNIRHNIKTDWIDKGFPVYK"
     gene            6243..6512
                     /locus_tag="VGM24_05825"
     CDS             6243..6512
                     /locus_tag="VGM24_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY4154920.1"
                     /translation="MNKAELIARISEDAGITKTQAGASLDSFTEAVAKSLKKGDKVTL
                     VGFGTFSVSKRAARNGRNPQTGATIKIKAKKVAKFKAGKELSARV"
     gene            6627..7091
                     /gene="bcp"
                     /locus_tag="VGM24_05830"
     CDS             6627..7091
                     /gene="bcp"
                     /locus_tag="VGM24_05830"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY4154921.1"
                     /translation="MTNLKAGDKAPVFSGKDQNGKKVSLSDYKGRRVIIYFYPQDDTP
                     TCTIEACNLRDNYSMLKKEGFVILGISPDSEKSHKKFAAKFDLPFTLIADPEHAIIDK
                     YGVWGEKQLFGREYMGVLRSTFLVDEHGMIRKIFEKPKNSAHAEEIIAGWNE"
     gene            complement(7069..>8018)
                     /locus_tag="VGM24_05835"
     CDS             complement(7069..>8018)
                     /locus_tag="VGM24_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HEY4154922.1"
                     /translation="ETAEVLEKLIRNDSDWRLKYHLGLIWWSRNNTGKAKELFSACGT
                     TPAYAPFYAAKAALFPEEGEMDLKRAIELDKEQWRYARLLTEYYLKQNEKEKALAVVE
                     SFYRRHPENYIMGMLYARTLLLNRQYASTDALLTRLDIIPFEGATGGRQLYHEAKLMQ
                     AVAAMKKKEYKKALAFIQASGKWPENLGVGRPYPEDIDERLENWLAYLCYLQLGNKTN
                     AEQALKNIIAFVPKRENTVSNYLSVNTLVTAWALEKMNRREEGLRLLDQWIQKDPSNK
                     MAAWCKQVFENQPAAVPPKDILKDEGVRIIEEFIHSTRQ"
BASE COUNT         2202 a         1951 c         1861 g         2004 t
ORIGIN      
        1 cttcccggaa cagacgggaa aacgtattcg ctggccagtt tcagcgggaa ggaggtgctg
       61 gtgatcattt ttacctgtaa tcattgtccc acggcccagg cctatgaaga ccggatcatt
      121 cagttaacgc gcgattattc cgcaaaaaac gtcgccgttg tcgccatcat gcccaacgat
      181 cctttatcca ttaacctgga cgagctggga tacaccgata tgggcgattc ctttgaagaa
      241 atgaaaatcc gggcgaaaga gaaaaaattc aatttccctt atctgtatga tggagatact
      301 gaaaagaccg cccgggctta tggtccggtc gcaacccccc atgtgtttat tttcgataaa
      361 aaacgaatcc tgcgatacag cggtcgcata gacgatatgg aaaagccgac caagacgccc
      421 acccggtacg atacccggaa cgctattgat gcactcctgg ccgggaaacc tgttccggtg
      481 accagcacca aggtcttcgg ctgttccatt aaatgggcgg aaaagagcga ctggataaaa
      541 gattcaaggg agaactgggc gaaagagccc gtccggttgg ataccctggg cgaagcagga
      601 ctccgggacc tgctcaaaaa ccattcagac aagttgaggc tgatcaatgt atgggctacc
      661 tggtgcgggc cctgcgtggc cgaattcccg gaattcatta ccatgaaccg catgtaccgc
      721 caacgtgact ttgaatttat cagcatcagt gccgatgaat tggcaaaaga ggcaaaagcc
      781 cttcaatttc ttcagaaaca acaggcttcc ggcaggaact atattttttc cggtgataat
      841 aagtataacc tgattgaagc catagacccg aactggcagg gagcgctacc ctatacgctc
      901 ctggtggaac ccggtggcaa aatagtattt gccagagaag gcgccatcga tcctttcgag
      961 ctgaaaaaag caattgtgga taacaaatat atcggccggt attactaatt ctcaactgct
     1021 ggttccttcc agcagcttgt acagctcatc cagtctcgga gataaaatga tttctgttct
     1081 ccggttgagc gcccttccct ccggagtact gtttgtctgt accggatcgt agaggctatg
     1141 accggaggag atgacccgga ccggatccac actataatcc tgggtgagga tgcgaacaat
     1201 ggaattggcg cgggctgtgc tcaggtccca gttgtccttg tatttaccgc ggatgggaat
     1261 ggaatcggtg tgtccttcta tatccactgt aatatccgga ttcgcattca ggacttccgc
     1321 cagtttcccg agcgcttcct tccccttagg gtttacgaca gcactgccgg aaggaaaaag
     1381 cagattctcc tgcaggctca catacacttt tccgtttttc acggaaaccg tcagttcatt
     1441 ggaattaaaa cccaccagcg cgtccgacat ctttttcctg atttcctgtg tggctttctt
     1501 ttgctgatcc atcaacgcct gtaattgttc cagccgtttt tgctgatctg ccagttcctg
     1561 ctggcttttg tttaattgcg acgctttatt ggcagcatcc gttgacaact ccccggcttt
     1621 attggtgaga tcgttcacct gccccttaag ctggcttacg gtggactcca gagtgctcac
     1681 ccgggcggcc agccgggcac tgtcgctccg cgccgattgc agcgcccgtt gggaggcgtc
     1741 aaatttcctt gtcggaacac aactgcccag gaataaggcc accgcaatca ttgcgatcag
     1801 gccattgatt ttttttgtca tggtaatcgt ataattaatt aaaattaaac agagatgaca
     1861 gcagcttccg cccgttactg atccggaagg gcttgccggg ctttactgca attgtcttgc
     1921 catactgccg cattccggtt tcaaaatacg gatccgccgg aaaaaatggt catcccctac
     1981 cagaacacgg tatataaagc ggataatatt ccgatgataa aaaccgaccc gacaataaaa
     2041 gcgggggaaa cggaaaactg cttcctgtcg atgaggatgc tatgcgccgc ctgatcggcg
     2101 gcaggttttg aaaggctgat gaatatcatt atgatgacga tcaaaataaa ggtgatggac
     2161 atgcggtcta aaaacggata atccggaaag gcgccgcccg tccaggacgg aagaaatttc
     2221 agaacagtgg aaatgggtat ggtcagaata gtcccggtaa gggcagctgc cgccgtagtg
     2281 cgtttccaga aaaaacccag caggaaaatg gccagcacgc cgggtgaaat aaatccaaca
     2341 tattcctgta tgaactgata tgcctggtcc agatttttca aggccggcgc aatcagtgcg
     2401 gcaattccca tagccacgat aaccacccat ttaccaattc ccaccaattt tttttcagag
     2461 gcttctttat tgaaaaattt cttatagata tccagggaga atatcgtcga aatactgttc
     2521 gccttgcccg ccaaagaggc caccaccgcg gcggtcagtg cagcaaacgc aactcccttt
     2581 aatcccgcag gcaacaaatt cattaaggtg ggataggcat gatctggttt gatgacgccg
     2641 ccctgcatca tttcgtgttg aaaaagcccg ttcttatgaa ggacgaacat ggtgatcccg
     2701 ggtaagacca caatcaccgg caccagtaat ttcagaaaag ccgcaaacag gatcccgctg
     2761 cgtgccgttt ttaaattggc ccccagggcg cgctggatga tatattgatt acatccccag
     2821 taagcgaggt tgttgatcca cattccgcca atcaaaaccg acaatcccgg aaggtcctta
     2881 taatagggat ttcccttgtg aaagatcata tgaaagtgat ccggcgcttc ctttctcaat
     2941 atggacaagc ccttccatac attgctcccg aaaccaaaat gtccggagag caggttaagc
     3001 gcaagataag tggtgaccag gccgcctacc agcaatacga gaacctggat cacatccgtg
     3061 tatccaatca ccttcatacc ccccagggtt acaataatcg aaaacgcgct caggcccgca
     3121 acactccagc caaaggaaat atcggaaatg gaagtgatgg ccagggcccc caaataaata
     3181 atagaggtca ggtttacaaa aacataaacc aggagccaga aaatcgccat catcgtactg
     3241 acctgatcgt tgtagcgccg ggcaagaaac tgcggcattg taaaaatttt attcttcagg
     3301 tagatcggaa gaataaatac agcgacgatg atcagcgttg cggcggccat ccactcgtag
     3361 gtggagatgg ccagtcccag ggcaaacccg gaacccgaca tgccaataaa atgttcggcg
     3421 gaaatattgg aggcaatcag cgatgccccg atggcccacc aggttagaga accttccgcc
     3481 aggaaaaagt ctttggaatc ggtggaagca aatttctttt tgtgataaac ataatagccg
     3541 taagcggaaa cgacgatgaa ataaataaag aatacgacat agtcggctgg ctgtaattta
     3601 tgcacgggtt attggctgat gggttgattg aaataaggac ctgcggaacg atcagaactt
     3661 cagcgttgcg ggcaaatatt ttgaaaccag gtcttcataa tatggcttta actccttcca
     3721 gtccggaacc gtcggatttt tggaataaag atcgtaaggg ttgaataatt taacccattt
     3781 cagcatttcc cggtcgtgtt catccagtaa gtaatcgtag gcgctctcgc ggtgccaggc
     3841 ataaaaggaa tgataacgga gcatatacaa ggctggttcc ggcagcgaat ccttcatcat
     3901 ctgatataca tattcatcat ggccccagga catgctgaca ttccttaacc cgcagttttc
     3961 cgaatacagc cctaatttcg aattaagcac ctcatccttt acatccggat ttaatgcaaa
     4021 aaactcgggg taaacaattt tttcggaatg ggcgcatccg accgggaacg tgtcgccgac
     4081 aacggcccac tggggttcgc caaacaggca gagcacttta cccatatcat gaattaatcc
     4141 gaccgttacc atccagtccg gatgaccatc cgcgcgaatg gcttccgccg tttgcagtaa
     4201 atgctggaac tgatccagat ccgtatccgg atcggaatca tccaccagct gatttagaaa
     4261 ctgaaaggca tcccagacgg gcatttccct tttattgaac tgtaaaaatt cggctctttt
     4321 ctcctgcaca aacgcgtaac tctgccaggt atgattcagg cggtagaatt cctttaccgt
     4381 atcccgggcg ggagcttcgt aattccggta ttcttccttc tgtttcgcca tggccggctt
     4441 gtccgcttcc ggatatcttt tcaacacatc ttcttcccat tcatccagac tggccagagg
     4501 agaaatttcg gtttgcgata aagattttga agaggacata ttcggcagtt taagtgagag
     4561 aataaaaaag ttttcaggag aacgatcgct gtgcggcccg gacgatataa caagcaggct
     4621 caatttacga aaggtttccg gggatcaaac cgtttctgcc tgaagaaatg aaaataggat
     4681 tttattaatt gaaaatcagc aatttgcact ttccatcggc cagggctgcg actcaaaaaa
     4741 aattcaggga atgcgtaaag aataataact ttattattcg cccgaaaatt cccggatcat
     4801 gataaaaacc atcacagcca ttttcctttt ttgcgctgtc tcggccaacg cccagcagca
     4861 taagctggta aaattgtggg agaccgattc cgttgttgcg atacccgaat cggtgttgcc
     4921 ggatttgaaa aataatatcc tgtacgtttc cctgatcgac ggaggtggct gggatgccga
     4981 cggcaaaggc ggaattgcca ggctgggtac ggatggtaaa cactatgaac ccaactgggt
     5041 caccggaatg aatgcaccca aaggcctggg gaaatacggt aatagaatat atgccgccga
     5101 tatctccgaa gtggtcgtcg ttgatatttc gcatagaaaa ataataaaga agattgcaat
     5161 agaaggagca agcggactga acgatataac ggtgtcggac aaaggaattg tatatgtttc
     5221 cgattccaaa acgtcaagga tctggaaaat agaaaatgac aaaccttctc tctacctgga
     5281 aaatgtcaat ggggccaatg gtttgaaagc cattaagaat gatctgtact atgcagaagg
     5341 ccccgttgtg atgaaagcgg acgcacaaaa aaaactgacc cgggtggcgg aagttcccca
     5401 gtccattgat gggatcgaac ccgtcggaaa cggagacttc atcgttaccg gctggtcggg
     5461 ttatattttt tatgttcatg cgaatggcgc ggtggacgcc ttactcgatt cgcacctgga
     5521 aaagataaat acggccgacc tcgggtatga tccggccagg cgcattttat acgtgcccac
     5581 cttcctggcc aaaaaagtaa tcgcgtacaa attaaattag actacggatc cggcggcagg
     5641 tatttctgtg ccgggaaaca gcataaccga tggcaagctt taaatccctt tctgtttttc
     5701 tattttttac ttctttatgc gtcctggcat tttctgttac cggctttcgg caaaaaacac
     5761 atacagaacc ctggacagaa gatcaattgc tggcaccgga agatctggcc cggactataa
     5821 atgacccgaa tgcaaaacaa ccggtcatcc tttgcgtcgg gcccggcgca atgataaaag
     5881 gatcgctgga tatgggtccg gcccatgaaa aagcgggcta tgaaaaactg aaggatcaac
     5941 tggaaaaatt gccaaaaaac gctgatatcg ttatctattg cggttgctgc ccgtttgacc
     6001 attgtcccaa tatccgcccg gcgtttgctt tattgaatga aatgaaattc aacaaccagc
     6061 ggcttttgaa tatccggcac aacatcaaaa cagactggat agataagggt ttcccggtat
     6121 ataaataact ttttgccagg ttaaaaaacg gatgggcttc aaaataaccg gtccgaaaag
     6181 tctctgccag gcgcatagcc cttatatttg ttctttaacg aatcaataaa aaaagtacat
     6241 ccatgaacaa agctgaattg atcgccagaa tttccgaaga tgccggcatt acgaagaccc
     6301 aggcaggcgc ctcgctggat tcttttacgg aggccgttgc aaaatcgctt aaaaagggag
     6361 acaaggtaac cctcgtaggt tttggcacat tctccgtttc taaacgggct gcccgtaatg
     6421 gccgcaatcc ccaaaccggc gcgacgatca aaataaaagc caaaaaagta gcgaagttta
     6481 aagcaggcaa agaactttct gcaagagttt aaattcatag tccaacgtat aaaaaggctg
     6541 gatcttcatc cggccttttt agctgaatat accgccgccg ccggcaaaat tgattatcga
     6601 agcggctttt atttcaattt atttttatga caaatttgaa agcaggagat aaggccccgg
     6661 tgttttccgg gaaggatcag aatggcaaga aagtttcctt atccgattat aagggcagaa
     6721 gagtgatcat ctatttttat ccccaggacg atacgccaac ctgcaccata gaagcctgta
     6781 atctccggga taactattct atgcttaaaa aagaaggatt tgttatcctg ggaataagtc
     6841 ccgacagtga aaaaagtcat aaaaagttcg cggcaaaatt tgacctgccc tttacgctga
     6901 tcgcagatcc ggaacatgct atcatagata agtatggcgt ctggggcgaa aaacaattat
     6961 ttggccgcga gtacatgggt gtgctccgca gcacgtttct ggtggatgaa cacggaatga
     7021 tccgtaaaat attcgagaag cccaaaaaca gtgcgcatgc ggaggaaatt attgccgggt
     7081 ggaatgaatg aattcctcta ttatcctcac cccttcatct ttcagaatat ctttaggcgg
     7141 tacggcggcg ggttgatttt caaaaacctg tttgcaccag gcagccatct tattcgaagg
     7201 atctttttga atccattgat ccaatagccg aagcccttcc tcccgccggt tcattttttc
     7261 gagggcccac gccgtaacca gggtattgac cgataaataa ttacttactg tattttctct
     7321 tttcggtaca aaggcaatta tgttttttaa ggcctgctcc gcattggttt tattccccag
     7381 ctgaaggtaa cacagatagg ccagccagtt ctccagccgt tcatcgatat cttccggata
     7441 aggcctgcct acccccagat tttcgggcca ttttcccgaa gcctggataa aggccagggc
     7501 cttcttatac tccttctttt tcattgcggc taccgcctgc atgagttttg cttcatgata
     7561 caattgcctg cctcccgtcg ctccttcaaa aggaatgatg tcgagcctgg tcaacagcgc
     7621 atccgtgctg gcatactgcc tgttcagcag caatgttctt gcatacagca tacccataat
     7681 ataattttca gggtgccgcc ggtaaaagga ttccaccacg gccaatgctt tttccttttc
     7741 attttgcttc aggtaatatt ccgtcagcag tctggcatat cgccattgtt ccttatccag
     7801 ttcaatcgcc ctttttaaat ccatttcccc ttcttccgga aacaatgcgg ctttggccgc
     7861 ataaaacggc gcataggccg gagtggtccc gcaggctgaa aataattcct ttgcctttcc
     7921 cgtattgttc ctgctccacc atattaatcc cagatgatat ttcaatcgcc agtcgctgtc
     7981 attcctgatc agcttttcga gaacttccgc tgtttcag
//
LOCUS       DASYCU010000290         5864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4558618, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000290 DASYCU010000000
VERSION     DASYCU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4558618"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VGM24_05840"
     CDS             <1..659
                     /locus_tag="VGM24_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4154923.1"
                     /translation="ITAVIIEGIQGVGGINLADPSFLQKIRSLCTQHNAVFIADSVQC
                     GYGRTGKFFYHDHAGVNADIYSMAKGMGNGFPVAGILIAPQFKSRHGMLGTTFGGNHL
                     ACAAALAVLEVIESEKLMENAGATGDFLKNELLKMDRLQNVRGKGLMIGFDVTGELKD
                     LRKNLLFNQQVFTGESKPSVIRLLPSLALRKKEAGEFLESLTDEINHLTSSPNLVTQT
                     "
     gene            656..1621
                     /locus_tag="VGM24_05845"
     CDS             656..1621
                     /locus_tag="VGM24_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HEY4154924.1"
                     /translation="MKNFISIADVVDLDKLIGKALYYKKNPFADKTLGEGKRIGLLFL
                     NPSLRTRLSTQVAARQLGLEPILFNVDKEGWALEFEEEAIMSGNTVEHVKDAAPVLGK
                     YFDILCIRTFPSLKNREDDYSELFIRQFVKYSGVPVVSLESATLHPLQSLTDLITITE
                     TLPAGPPAGKPKIVLTWAPHVKPLPQCVANSFSQWINAWGKSDFIITHPEDYELDPGF
                     TRGAVITHNQNEALKDADFVYVKNWSTYQDYGKIYTNDPEWMLTNEKLKNTRQAKVMH
                     CLPVRRNVELSDEVLDGSHSIVTRQAGNRVWAAQAVLSDILQSQF"
     gene            1625..2410
                     /gene="argB"
                     /locus_tag="VGM24_05850"
     CDS             1625..2410
                     /gene="argB"
                     /locus_tag="VGM24_05850"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY4154925.1"
                     /translation="MEDLYVIKIGGNIIDDESILESFLKKFAVIPGKKILVHGGGKLA
                     TRMAEKMGIPQQLVNGRRVTDAETLKIVTMVYAGYINKKIVARLQSLDCHAAGICGAD
                     GNSILAHKRIPSGTDYGFAGDIDSVNAAWLGSLLNQGLTVVMAPVTHDGKNQLLNTNA
                     DSIAQEIAKAMSRSFRVQLLYSFEKAGVLLNVEDENSVIHSLTPAYYQELKESKKIFA
                     GMIPKLDNAFAAIRNGVNKVIIGKGEDLGNLLNGKSGTTISHE"
     gene            2403..3476
                     /locus_tag="VGM24_05855"
     CDS             2403..3476
                     /locus_tag="VGM24_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEY4154926.1"
                     /translation="MSKLSIEALQSEAIRLLKDLISTPSFSKEENDTAELISAFFEKH
                     EVPFSRVGNNIYARNKNYQTSRQSILLNSHHDTVRPNKNYSMDPFTPTEKDGKLFGLG
                     SNDAGGCLVSLIAVFLHYYHSEDLKHNVVFAASAEEEISGVNGIELVLPYLGQIDFGI
                     VGEPTRLEMAVAERGLMVIDCVAAGRPGHAARNEGENALYKALDDIDWIRNYRFEKKS
                     DLLGETRMSVTVIDTENKQHNVVPSQCKFVIDVRVNELYSFEEILDALKNNLKSHFKP
                     RTTRMKSTSIPLSHPLVKAGTALGRGYYGSPTTSDKALMPFPALKMGPGDSARSHTAD
                     EFIYIEEIKEGIAVYIQLLNEFI"
     gene            3523..4824
                     /gene="argH"
                     /locus_tag="VGM24_05860"
     CDS             3523..4824
                     /gene="argH"
                     /locus_tag="VGM24_05860"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY4154927.1"
                     /translation="MKLWQKEELKVNELIENFTVGRDKEFDMVLAGYDVEGSLAHARM
                     LESIGMLTKEESRSIQKALQHMLADIREGRFQIEPGVEDVHSQVEFNLTKAIGEAGKK
                     IHSGRSRNDQIALDIKLFLRAEIQNIKTEAKELFDLLIFLSNQCQGDLLPGYTHLQIA
                     MPSSFGLWFGAYAESLIDDLEMLAGSFQVTNKNPLGSGAGYGSSFPLNRRMTTSLLEF
                     SSLNYNSVYAQMTRGKTEKLVAFGMASIAATLSRLAMDCCLYMNQNFGFISFPDELTT
                     GSSIMPHKKNPDVFELIRAKCNRIQSIPNELSLLLVNLPSGYHRDLQLSKEILFPAIE
                     ELKACLQMTGYMLSNIRITKDILKNEKYKYLFSVEAVNELVNRGIPFREAYQTVGQQI
                     ANGSFSFDGTRPLNHTHEGSLGNLCNPEIVAEMNKVLTKFK"
     gene            4906..5409
                     /locus_tag="VGM24_05865"
     CDS             4906..5409
                     /locus_tag="VGM24_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HEY4154928.1"
                     /translation="MKFRIVFRLFMPLCLLLPAVISAQTKEDKKKAQYEELKAHIDAR
                     QYTFNAQSATSMGGRTRQLSSLYTLKIHQDSLDADLPYFGRAYSAPLDNSNGGIHFRS
                     VSFGYEQTSSKQGGWVIRITPAGERSASNMQMTISSTGYATLQVNSNDRQMISFYGRV
                     SGNSKKK"
     gene            complement(5413..>5864)
                     /locus_tag="VGM24_05870"
     CDS             complement(5413..>5864)
                     /locus_tag="VGM24_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY4154929.1"
                     /translation="FTAEEIGEFGSAYFSRQFNPDKVMAMFNIEMIGTESKWGKNSVY
                     LTGYDRSDMGSIMQKNLKNTVFQLHPDPYPEQQLFFRSDNASLAKLGVPAHTVSTSKM
                     DSEKYYHTRGDEIGTLDLQNMTGIIRAIAWSAGTIIAGTDTPRRVKP"
BASE COUNT         1571 a         1435 c         1523 g         1335 t
ORIGIN      
        1 aaattacggc ggtgatcatc gaagggatac agggcgtggg cggcatcaac ctggcagatc
       61 cttctttcct gcaaaagatc cgctcgctct gtacgcagca caatgccgtt ttcatagcag
      121 acagcgtcca gtgcggatac ggaagaaccg ggaaattttt ctaccatgat catgccggcg
      181 tgaatgcgga tatatacagt atggccaaag gaatgggaaa cggttttccc gttgccggca
      241 ttctgatcgc gccgcagttc aaatccaggc acggtatgct gggcaccacg ttcggcggga
      301 accacctggc ctgcgctgcc gcgctggcgg tgctggaagt gatcgaatca gaaaaactga
      361 tggaaaatgc aggggcaacc ggcgatttcc tgaagaatga attactgaaa atggatagac
      421 tccagaatgt acgcggcaag ggattgatga tcggctttga tgttacgggt gaattaaaag
      481 acctgcgcaa aaatctctta ttcaaccagc aggtctttac cggggaatcc aaacccagcg
      541 tgatccgttt gctcccttcg ctggccctga ggaaaaagga agccggggaa ttcctggaat
      601 ctttgacgga tgagatcaat catttaacat catctcctaa tctggtaact cagacatgaa
      661 aaattttatt tccattgctg atgtggttga cctggacaaa ctgatcggca aagcacttta
      721 ctacaagaaa aatcctttcg ccgataagac actgggagaa ggaaaacgga tcggactgct
      781 gttcctgaac ccgagcctcc gcacccggct gagcacacag gtggctgccc gtcagctggg
      841 tttggagccc atcctcttta acgtggacaa agaaggatgg gcactggaat ttgaagaaga
      901 ggccatcatg agcggcaaca ccgtggaaca cgttaaagat gccgcgccgg tgctgggaaa
      961 atattttgac atcctttgta tccggacctt tccttccctg aaaaaccggg aagatgatta
     1021 cagcgaatta ttcatccggc agtttgtcaa atattcgggc gttccggtag tcagcctgga
     1081 aagcgccacc ctgcacccgc ttcagagcct gacagacctc attacgatta cagagacgct
     1141 gccggcaggc cctccggccg gaaagcccaa aatcgtgctg acctgggcgc ctcacgttaa
     1201 accgcttccg caatgtgtgg ccaatagttt ttcacaatgg atcaatgcct ggggcaagtc
     1261 ggatttcatt atcacgcatc ccgaggatta tgaactggat cccgggttta ccaggggggc
     1321 tgttatcacc cataaccaga atgaagcgct taaggatgcc gattttgttt atgtgaaaaa
     1381 ctggagcacc taccaggatt acggtaaaat atataccaac gacccggaat ggatgctgac
     1441 caatgaaaaa ttaaaaaaca cccgtcaggc aaaagtgatg cattgccttc cggtgcgcag
     1501 gaatgtggag ctgagcgatg aagtcctgga cggatcccac agcatcgtca cgcgccaggc
     1561 cggaaaccgg gtatgggcgg cccaggcggt attgagcgat attcttcaat cccaatttta
     1621 accaatggaa gacctgtacg tgatcaagat cggggggaat atcattgatg acgaatccat
     1681 actcgagagc tttctgaaaa aattcgccgt cattccgggg aaaaaaatac tggttcatgg
     1741 cggaggcaag ctggctaccc gcatggcgga aaaaatgggc attccccagc agctggtgaa
     1801 cgggcgccgg gtgaccgatg cagaaaccct gaagatcgta accatggtgt atgcgggcta
     1861 tatcaataaa aagattgtcg cccggctgca gtccctcgac tgccatgcgg ccggcatctg
     1921 cggtgcagac gggaacagca ttttggcgca taagcggatc ccttccggaa cagactatgg
     1981 atttgccgga gacatcgatt cggtcaatgc ggcctggctg ggcagcctgc tgaaccaggg
     2041 attgaccgtt gtaatggccc ccgtcacgca cgacggtaag aatcagttgc tgaataccaa
     2101 cgcagacagc attgcgcagg aaatcgccaa ggccatgagc agatcgtttc gcgttcagct
     2161 gctgtacagc ttcgaaaaag ccggcgttct gctgaatgtg gaagatgaaa attcggtaat
     2221 tcattccctt accccggcct attaccagga attaaaagaa tctaaaaaaa tattcgcggg
     2281 aatgatccct aaactggata atgcttttgc ggccatccgg aacggggtca ataaagtcat
     2341 catcggaaaa ggagaggacc tcgggaatct attgaacgga aaatcaggaa caaccatcag
     2401 tcatgagtaa attatccata gaagcgctgc agtcggaagc catccgcttg ttaaaggatc
     2461 tgatttccac gccctctttt tcgaaagaag aaaacgatac ggcggagctc atcagtgcct
     2521 tttttgaaaa gcacgaagtt cctttttccc gggttggaaa taatatttat gcccggaaca
     2581 aaaactatca aacctcccgg cagagtattc ttttaaactc ccatcacgac acggtccgac
     2641 ccaacaagaa ttacagcatg gatccgttca cgcccaccga gaaagacggg aagttgttcg
     2701 gcctggggag caacgatgcc ggcggttgcc tggtcagcct cattgcggta tttctccact
     2761 attaccacag cgaagacctg aaacacaatg tggtttttgc cgccagtgcg gaagaagaga
     2821 tcagtggcgt caacggaatt gaactcgttc ttccttacct tggccagatc gattttggta
     2881 tcgtgggcga acccacccgg ctggaaatgg cggttgccga aagaggcctg atggtgattg
     2941 attgcgtggc ggccggcagg cccggtcatg cggcgaggaa tgaaggggaa aatgccctgt
     3001 ataaagcgct tgacgatatc gactggatcc ggaattaccg cttcgaaaag aaaagtgacc
     3061 tgctcgggga aacacgcatg agcgtaaccg ttatagacac ggaaaacaag cagcacaatg
     3121 tggttccgtc ccagtgtaaa tttgtgattg atgtccgggt gaacgaattg tacagctttg
     3181 aggagatcct ggatgcgctg aagaataacc tgaaaagtca tttcaagcca aggaccaccc
     3241 ggatgaaatc cacctctatc ccgttaagcc atccgctggt gaaagcgggt acggcgctgg
     3301 gaagagggta ttacggatcg cccaccacat ccgacaaagc gctgatgccc tttcccgccc
     3361 tgaaaatggg tccgggggat tcagcgcgga gccatacggc agatgaattt atttatattg
     3421 aggaaattaa agagggaatc gccgtgtata tacagctgtt gaatgagttt atataggctg
     3481 tttatgattg tccatttaat gggtaaggtt aaaatttaaa cgatgaagct ctggcaaaaa
     3541 gaggaattaa aagtcaatga actcattgaa aacttcacgg tgggccggga taaggaattc
     3601 gatatggtat tggccggata tgatgtggaa ggatccctgg cgcatgcccg catgctggaa
     3661 agcattggca tgctgacgaa ggaagaaagc cggtccatac aaaaagccct gcagcatatg
     3721 cttgccgata tccgggaagg ccgttttcag attgaacccg gtgtggaaga tgtgcattcc
     3781 caggtggaat ttaatctcac caaggccatc ggcgaagcag gaaaaaaaat tcacagcggc
     3841 cgcagccgga atgaccagat cgcgctggac atcaagctct ttctccgcgc cgaaattcaa
     3901 aatatcaaaa cggaagcaaa ggagctgttt gatctgctga tctttctgag caaccagtgc
     3961 cagggcgatc tgctgcccgg gtatacccat ctgcagattg ccatgccatc ctctttcgga
     4021 ttatggtttg gcgcctatgc cgaaagcctt atagacgacc tggaaatgct ggcaggctct
     4081 ttccaggtga ccaataaaaa tccattgggg agtggggcgg gttatggttc ttctttccct
     4141 ttaaaccggc ggatgaccac ctcgctgctg gaattctcct ccctgaatta caattcggtg
     4201 tacgcccaga tgacccgggg gaaaacagag aaactcgtgg cctttggaat ggcttccata
     4261 gccgcaacgc tctcccgcct ggccatggat tgctgcctgt acatgaacca gaatttcggg
     4321 tttatttctt ttccggatga actgaccacg ggcagcagca tcatgccgca taaaaaaaac
     4381 ccggatgtct ttgaattgat ccgggcaaaa tgcaaccgga tccagtccat ccccaacgaa
     4441 ctcagcctgc tcctggtaaa tctgccttcg ggttatcacc gggatttgca gctcagcaag
     4501 gagattctat ttcctgccat agaggaattg aaagcatgcc ttcagatgac cgggtatatg
     4561 ctatccaata tccggatcac aaaggatatc ctgaaaaatg aaaaatataa atatcttttt
     4621 agcgtggaag cggtgaatga actcgttaac cggggaatcc ctttccggga agcctatcag
     4681 acggttggcc agcagatcgc caacggaagc ttttcctttg atggtacgcg tcccctgaac
     4741 cacacccacg aagggagcct gggcaatctt tgcaacccgg agatcgttgc tgaaatgaac
     4801 aaggtgctta cgaagtttaa atagcccggc ggcgccggtc cgggatggct gaaagccggc
     4861 tgattccgtt agaaacggga tttcctacct ttacaaaaaa gttatatgaa attcaggatc
     4921 gtattccggc ttttcatgcc gctttgtttg ctactccccg ccgtcatttc cgcccagacc
     4981 aaagaagata aaaagaaagc gcagtatgaa gagctaaaag cgcatattga tgcccgccag
     5041 tatacgttca atgcacaatc cgccacttcg atgggtggaa gaacccgtca gctgagcagt
     5101 ctgtacacac taaaaattca tcaggacagc ctcgatgcag acctgcccta tttcggacgg
     5161 gcttattccg cacccctgga caatagcaat ggaggcatcc atttcaggtc cgtttccttt
     5221 ggttatgagc agaccagttc aaaacaggga ggatgggtga tccggattac cccggcaggg
     5281 gaaaggagcg ccagtaatat gcaaatgacc atcagctcaa cggggtatgc cacccttcag
     5341 gtgaacagca acgaccgcca gatgatctct ttctacggtc gggtatcggg gaattcaaag
     5401 aaaaaataga cctcagggtt tgacccttcg cggggtgtcc gttccggcaa tgatggtgcc
     5461 cgcgctccag gcgatggccc ggatgatccc ggtcatattc tgcaggtcca gggtcccgat
     5521 ttcatctccc cgtgtgtgat aatatttttc cgaatccatt tttgaagtgg acacggtatg
     5581 cgcgggaact cccagtttag ccagcgaggc attgtctgaa cggaaaaata actgctgctc
     5641 cggataagga tccggatgaa gttggaacac ggtgtttttc aaattttttt gcatgatgct
     5701 tcccatatcc gaccggtcgt atcccgtgag atacaccgaa tttttacccc atttgctttc
     5761 cgttccgatc atttcgatat tgaacatggc catgacctta tccgggttga attgcctgga
     5821 aaaataggcc gaaccgaatt caccgatctc ttccgccgta aacg
//
LOCUS       DASYCU010000291         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1078121, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000291 DASYCU010000000
VERSION     DASYCU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078121"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..927)
                     /locus_tag="VGM24_05875"
     CDS             complement(196..927)
                     /locus_tag="VGM24_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY4154930.1"
                     /translation="MKEMLRIGIADCGKYENYRRWIEAGGNAQAVRLSMNLNNPEAVA
                     GCDGVVLSGGEDVQPELYGKPEYVQEYGLKEIIPERDRFEYEVIRNTLDQGKPLLGIC
                     RGLQVVNVYLGGNLVPDIPAILNSDWHGKKQGIDQVHPVKIIRDSLLNKLSGLDSGQV
                     NSAHHQSAGQPARDLKPTAFADPGIIEAMEWAEPRNRSWLLLVQWHPERMQDPSGPLT
                     AGLRKAFLEACKIGEQGYSTSTGKM"
     gene            complement(978..2318)
                     /locus_tag="VGM24_05880"
     CDS             complement(978..2318)
                     /locus_tag="VGM24_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4154931.1"
                     /translation="MFKRFELRILIRVIFLFAVLCVTAAVVIEAQPIYLIILLPVLAY
                     QFSDFYRFFRKTQDELDQFVESVHYRDFSRHFDVNRAPDELKTMRRGFNDINSTFKII
                     SREKETQFLYLQKILELVETGILSYEMESGNVVWMNDSLKRILGIPYLKTMQSLEKRD
                     AYLFKEINQLQPGIGKIVSISRDNGIVKVLASATAFQTDSKMFKLAAFQNVNEALDET
                     EAKAWQKLLSVMTHEIMNSVAPISSLADTMLDRLQKATELMREQPSAIEDLELGIETI
                     RRRSEGLLKFAETYRNLNKIVAPTVKRTLLRDLFENLHQLMLPTLDQKKVLLEVILQN
                     PELSYELDATLIEQVLINLLVNAIEAVKDKPNPRIQLSAFNASNNKINIKVADNGCGI
                     PASLMDRIFIPFFSTRKNGSGIGLSLCQQILMLHKGSIQVQSVENEGSVFTLHL"
     gene            complement(2375..>3102)
                     /locus_tag="VGM24_05885"
     CDS             complement(2375..>3102)
                     /locus_tag="VGM24_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY4154932.1"
                     /translation="LFESELFGHKKGAFTDARDDRTGRFEMASGGTLFLDEIGNISLQ
                     QQAKLLSVLQSRQVSRLGSNLLVPIDIRLICATNLPISELANETRFRKDLIYRINTVE
                     IVMPPLRKRPEDIAPLAREFAHMYAGKYLKRTLELDNKAVEKLKAYHFPGNVRELQYS
                     IERAVIMCEGEEIHAEDLIFSPLETVREMEETEDRDLTLSSIEKNTILKVIEKHQGNI
                     SRAARELGLTRTALYRRLSKYDI"
BASE COUNT          680 a          802 c          788 g          832 t
ORIGIN      
        1 atcctcaaca gaaatctttt ccagatccta tttagttata aataaggttc acaacaggta
       61 tggaactgtt ttccggaggg acgaggactg gctttaggtg gatgggattt tgggagaacc
      121 ggcggaaatt gacaggatat aaaacaaaaa taggatagtc cggtgaataa aaaccaactg
      181 gttttttaaa tttatctaca ttttaccggt ggaagttgaa taaccctgtt cccctatttt
      241 acaggcttcc agaaaggcct tccgcagccc ggcggtcagc gggccggagg ggtcctgcat
      301 ccgttccgga tgccattgaa ccagtaataa ccaggagcgg ttccggggtt ctgcccattc
      361 catggcttct atgattccgg gatccgcaaa tgcggttggc ttcaggtccc gggcaggctg
      421 cccggcggat tgatggtggg cggaattcac ctggcccgaa tccagtccgg acagcttatt
      481 caacaaggaa tcccggatga tctttaccgg gtggacctgg tcaatcccct gctttttccc
      541 atgccaatcc gaattcagga tggctggaat gtccggaaca agatttccgc ccaggtaaac
      601 attaaccacc tgaagacccc ggcagatgcc cagcaggggt ttcccctggt ccagggtatt
      661 ccggatgacc tcgtattcaa aacggtcccg ttccgggatg atctccttca acccgtattc
      721 ctgaacatat tccggtttgc cgtataattc cggctgaaca tcttctcccc cgctcaatac
      781 caccccgtcg caaccggcaa ccgcttccgg attgttcaga ttcatgctga gccggacggc
      841 ctgcgcattt ccgcctgctt cgatccagcg ccggtaattt tcatatttcc cgcaatccgc
      901 aattccaatg cgaagcattt ccttcattgg gttatttttt aatttggagc ggcttccggg
      961 tgcagcccct gtttagccta taagtgcaaa gtaaatacag atccttcatt ttcgacggac
     1021 tggacctgga tgcttccttt atgtaacatg aggatctgct ggcaaagact gaggccaatc
     1081 ccgctgccgt ttttccgggt gctgaaaaag ggaatgaaaa tccggtccat gagggaagcc
     1141 ggtatgccgc atccgttatc ggccactttg atattgatct tgttattgct ggcattgaag
     1201 gccgagagtt gtatccgcgg gttgggcttg tccttaaccg cctcaatggc attgaccagg
     1261 aggttgatca ggacctgttc gatcagggtg gcgtccagtt cataggacaa ttcgggattc
     1321 tgcagaatga cttctagcag cacttttttc tgatccaggg taggcagcat cagctgatgc
     1381 aggttttcaa acaaatccct gagtagtgtt cttttcacgg tgggcgccac aatcttgttg
     1441 aggttccggt aggtttctgc aaatttcagc agcccttcgc tccgtctgcg gatggtttca
     1501 attcccagtt ccaggtcttc gatcgcggaa ggttgttccc gcatgagctc tgtggccttc
     1561 tgaagccggt cgagcatggt atctgccagg gaagaaatag gcgccacgga gttcatgatc
     1621 tcatgggtca tgacgctgag caatttctgc caggctttgg cttccgtttc atccagcgct
     1681 tcattcacgt tctgaaaggc ggccagtttg aacattttac tatccgtctg aaaggccgtg
     1741 gccgaggcca gcacctttac gatcccgtta tccctggaga tggatacgat cttccctatg
     1801 ccgggctgca gctggttgat ctccttaaac aggtaggcat cccttttttc cagtgactgc
     1861 atggttttca gatacggaat gcccaggatc cttttcaggg aatcattcat ccaaaccacg
     1921 tttccgcttt ccatctcata ggaaagaata cccgtttcca ccagctccag gatcttctgc
     1981 aggtagagga actgggtttc tttttcccgg ctgattattt tgaacgtgct gttgatatca
     2041 ttgaaccccc ggcgcatggt tttcagttca tcgggtgcgc ggtttacatc aaaatgacgg
     2101 gagaagtccc ggtaatgcac cgactccacg aactgatcca gttcgtcctg ggtcttccgg
     2161 aagaagcggt aaaaatcgct gaactggtag gccagcacgg gaagtaaaat gatcaggtaa
     2221 atcggctggg cctcaatgac caccgccgcc gtaacgcaga ggacggcaaa caggaagata
     2281 acgcggatca gtatgcgaag ttcaaatctt ttgaacatat ttttttactt tgtccaccga
     2341 tggatttttt tcaaccgccg gagctgctgg tgatttaaat atcatattta ctcagccggc
     2401 ggtacagggc cgtccgggtg agtccgagtt ccctggccgc tctcgaaata ttcccctggt
     2461 gtttttcaat cacttttaaa atagtgtttt tttcgatgct gctgagggtg aggtcccggt
     2521 cttccgtttc ctccatttcg cgcaccgttt ccaggggaga gaagatcaga tcctccgcat
     2581 gaatttcttc tccttcgcac atgatcaccg cccgctcaat actatactgc aattcccgga
     2641 cgttgccggg aaaatgatag gctttcagct tttcaacggc cttattgtcc agttccagcg
     2701 ttcgtttcag atacttacct gcatacatat gcgcaaattc ccgggccagg ggggcaatat
     2761 cttccggcct tttgcggagg ggcggcataa cgatttcaac ggtattgatc cggtagatga
     2821 ggtctttccg gaaacgggtt tcgtttgcca gttccgaaat gggcaggttg gtggcgcaga
     2881 tcagccggat gtctatcgga accagcaggt tcgatccgag acgcgagacc tgccggctct
     2941 gcagcaccga caatagtttc gcctgctgct gcaggctgat attgccgatc tcatccagga
     3001 aaagggttcc gcccgaggcc atttcaaatc tcccggtgcg gtcgtcccgg gcatcggtga
     3061 aggcgccttt tttatgcccg aacaattcac tctcgaataa gg
//
LOCUS       DASYCU010000292         7711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1661653, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000292 DASYCU010000000
VERSION     DASYCU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661653"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /gene="rlmN"
                     /locus_tag="VGM24_05890"
     CDS             complement(<1..784)
                     /gene="rlmN"
                     /locus_tag="VGM24_05890"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY4154933.1"
                     /translation="MKTDKRNIRTLSKEEIAEYLNQLGEPKFRAGQVYEWLWQKHAFS
                     FADMTNLSKELRAKLAADFAFPSLRIETTQYSADGTIKNRFKTFDNHFVEGVLIPATE
                     RRTACVSSQIGCSLSCKFCATGYMERERNLDFDEIYDQVVLLNQQSEKAHDKKLSNIV
                     FMGMGEPLLNYRNVLKSIERITSPEGLGMSPRRITVSTAGVSKMIRQLGDDKVKFKLA
                     LSLHAANDAKRHEIMPINDSNNIRSLVDALNYFYQQTGNVITF"
     gene            890..2089
                     /locus_tag="VGM24_05895"
     CDS             890..2089
                     /locus_tag="VGM24_05895"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY4154934.1"
                     /translation="MKKVFVIDAVRTPIGKYGGGLAALRPDDLLAYVIRALLNRNESI
                     DPEAIEDVIAGDANQAGEDNRNVARMAVLLAGLPVSVAGNTVNRLCASGLQAIMDASR
                     AIMCGEGDLYIAAGVESMSRAPFVMAKSAQPYNRKLEVMDTTIGWRFPNKRMTDVYYP
                     YSMGETAENVAKRWQISRTAQDEFALESQLKYFAALAAGKWDEEIVAVELERDRLISW
                     FSKDEHPRETSLEKLSRLKPAFIKDGTVTAGNSSGLNDGAAAMLLASEEGVAHYNLEP
                     MAQVLSMGVAGVDPAIMGLGPVPASQKALYRAGLTVNDMDLIELNEAFASQAIACIHD
                     LDLDLQKINVNGGAIALGHPLGCSGVRIATTLVHEMRRRESHYGMASMCVGVGQGAAV
                     VFENAGR"
     gene            complement(2092..3501)
                     /gene="hisS"
                     /locus_tag="VGM24_05900"
     CDS             complement(2092..3501)
                     /gene="hisS"
                     /locus_tag="VGM24_05900"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY4154935.1"
                     /translation="MNKPGLPQGTRDFGPETVYKRQFIFNTIRRVFELYGYEPLETPA
                     LENLETLLGKYGEEGDKLLFKILNNGLDHSSKKDSAAAEFEKVLAGKNNPLITERALR
                     YDLTIPFARYVAMHHNELSFPFKRYQLQPVWRADRPQKGRYREFYQCDADVVGSRSLL
                     NEAELTRIYLRVFEELKLEVEVRINHRKILAGLAALCGGQENLVSISTAIDKLDKIGI
                     DKVKQELGERKLSDRQISLVQQYLGIQGTNEEKLLQAGNLLNAAPGSDPLHKGKVGSA
                     SEGIREIQEVLNFFPGSKNIVLDFSLARGLNYYTGIIFEVRAGKVNIGSIGGGGRYDD
                     LTGLFGVPGIPGVGISFGVDRIYDAMEELRLFPEAVKRSTEFLFFNLGPEAVARCFEL
                     MEQLRGAGIRCELFPDESRFDKQFRYAEKKSIPFVVIIGTEELKTGKAKIKDLSTGIQ
                     EEQGFENLLSYARRITQHK"
     gene            complement(3511..3984)
                     /locus_tag="VGM24_05905"
     CDS             complement(3511..3984)
                     /locus_tag="VGM24_05905"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEY4154936.1"
                     /translation="MKILMVCLGNICRSPLAEGILEKKAKNEGLPWMVDSAGTNNGYH
                     VGEAPHPLSQKVASLHELDISTQCARSFVPEDFDRFDKIYAMSSDVLREMKAISGKKF
                     NVSKTDLLLNEVYPGENRDVPDPWYGDEPGYHIVYDLLDKACDRIIQKYGHLKTA"
     gene            4122..4934
                     /locus_tag="VGM24_05910"
     CDS             4122..4934
                     /locus_tag="VGM24_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY4154937.1"
                     /translation="MDPLAILYTDFLDLLFENRNKSYGAYPLRKYYPKRFFIAVGIVF
                     GIVLIFASAVILSGNKEFISSRIMYGPDPHLTRVVLEPRVINEPHALKPAAAGKRLAP
                     VSSGPPVIVKDQHIPRPIVTGEELNMAPSGASAEAGPPVTGGPVKQGAASSGGIDSAQ
                     ARPEILDRAQIMPEFPGGLEALRRFLLRNLRIPDSVTEPGSEVRVLVKFVVGTDGKVT
                     GIQLVQSGGMAFDPEVQRVISRMPVWKPGIQNGRKVSVYYNLPVNFVIPPDN"
     gene            5017..5253
                     /locus_tag="VGM24_05915"
     CDS             5017..5253
                     /locus_tag="VGM24_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154938.1"
                     /translation="MADDQDPDIRSKRYMLRRSIMDYGMGAIIFCLGIFFLIAPKVGV
                     IFGIDDLFRYLFGGLCILYGLFRVYRGYKKNYFN"
     gene            5265..6185
                     /locus_tag="VGM24_05920"
     CDS             5265..6185
                     /locus_tag="VGM24_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4154939.1"
                     /translation="MNIWKRCMPLILLAFLELSFSGCGHRKRIPVETATSGTIHISVD
                     EAFKPVIDSQLKVFESSFPDAKIIVEYKPEVNCLRDLEKDSTRMIIVTRGLSLKEEAF
                     YRDSLQRPAISGILAYDAVALIVNAHSRDTVMSMPEIRSLLEGNDKKGRQPVMDGVSA
                     TSTVRFAIDSILGGKPLGKNVEAARSSPQVIDYVASHENAVGFIGVSWIGDEEDSVQL
                     SFLQKVKVVAVECAGCPEKPYVQPFQANIVLKRYPMVRGLYYILKENYSGIGNNFVNF
                     LQNERGQLIFRRAYLVPARMSFNVRDVQLQ"
     gene            6235..>7711
                     /locus_tag="VGM24_05925"
     CDS             6235..>7711
                     /locus_tag="VGM24_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4154940.1"
                     /translation="MRKLSISLLLSVICIGSTVTAQTVEQGKQFLYYERYKSAQDAFE
                     KVLAANPNNIDAVYWLGQTMIHRRDTRDTAGAKALYQKMLATNGDAPLLLVGMGHVEL
                     LYGNTTDSRQRFERAISLTKEKDVNVLNAVGYANTDAKAGDVNYAIQKLTLATQTRKF
                     NNAETYVLLGDAYRKMLDGGNAVLNYNKALSLDPKLSEAKMKICIIYLTQNNRDYFLP
                     AFEDAIRIDPAYAPAYQQLFIYWYSRDVNKASAYLDKYLANSDQGPEAEYLKADMLYA
                     SSKFADARTRAQQLITQNGDKVIPRMYRMVAYTSDTLGDLPTAKTAMANFFSKADTSA
                     ERGLDYKILPTDYEEYAKIFSKMPDSASQVQAFQYYNMAIAMDTLPANKEKYANAGLE
                     LAKKLKNPQAAATLSDAMYKAIKEPNQSDLYKYGMANYSAGNYKKADSIFCGVYESKY
                     PTEIYGYLWCAKSKQAQDDSTNSQGLAVEAYDKLATFARGIA"
BASE COUNT         1880 a         1850 c         2044 g         1937 t
ORIGIN      
        1 cgaaagtgat cacgttcccg gtctgttggt agaagtaatt aagcgcatca acgagcgacc
       61 ggatattatt gctgtcgttg atcggcatga tctcgtgccg tttggcatcg tttgcggcgt
      121 gtaaggaaag cgccagtttg aatttcacct tgtcgtctcc cagttgtctg atcatcttgc
      181 ttacgccggc agtggaaacg gtgatccggc ggggactcat tccaagccct tccggtgaag
      241 tgatccgttc gatcgatttc agcacattgc ggtaattgag caggggttct cccataccca
      301 tgaatacaat attgctgagt tttttatcat gcgccttttc ggactgctgg tttaacagga
      361 ccacctggtc gtagatctcg tcaaaatcaa gattacgttc tctttccata tatccggttg
      421 cgcaaaattt gcaactcagg gaacagccga tctgggaaga aacgcaggcg gtccgccgtt
      481 cggtagccgg aataaggact ccttccacga aatgattgtc gaaggttttg aaccggtttt
      541 taatggtccc gtctgcgctg tactgggtcg tttcaatgcg cagggacgga aaggcaaaat
      601 cggctgccag cttggccctc agttccttac tgaggttggt catatccgcg aatgaaaaag
      661 catgcttctg ccacagccac tcatacacct ggccggcgcg gaatttcggt tcgcccaact
      721 ggttcaggta ttccgcgatc tcctctttgg aaagggtcct gatgtttcgc ttatctgttt
      781 tcatgaagcg gcaaagataa tcagtatttg cgggcgttaa gaaggaatag ggtcgccccg
      841 ccggataaag gggaaattca ttgatatttg ccttcattat acagtttcca tgaaaaaagt
      901 gttcgtcatt gatgcggtaa ggacccccat cggaaaatat gggggcgggc tggctgccct
      961 gcggccggat gatctgctgg cctacgtgat ccgggccttg ctgaaccgga atgaatctat
     1021 tgatcctgaa gccattgagg acgtaatagc cggggatgcc aaccaggctg gcgaagacaa
     1081 ccgcaatgtt gcccggatgg ccgttttact ggccgggctg ccggtatccg ttgccggaaa
     1141 taccgtgaac cgattatgcg cttccggtct ccaggccatc atggatgcct cccgggcgat
     1201 catgtgcggg gagggcgacc tgtatatcgc tgccggcgtg gagagcatga gccgcgctcc
     1261 ctttgtaatg gcaaaatccg cccagcccta caaccggaaa ctggaagtga tggacaccac
     1321 gatcggctgg cgttttccga ataaaaggat gacggatgtc tattaccctt attccatggg
     1381 agaaacagcg gagaatgtgg ccaaacgctg gcagatatcc cgtacggcgc aggatgagtt
     1441 tgccctcgaa tcccagttga aatattttgc cgcgctggcg gcgggaaaat gggatgagga
     1501 aatagtggcc gtggaactgg aacgcgaccg gctcatcagc tggttttcca aagatgagca
     1561 tccgcgcgag acctcactgg aaaagctgtc ccggctcaaa ccggcattta ttaaagacgg
     1621 aacggtaacg gccggaaact cttccggact caatgacggc gccgccgcga tgttgctggc
     1681 gagtgaagaa ggggtcgctc attataatct cgagcctatg gcgcaagtgc tctccatggg
     1741 agtggccggt gtggatccgg ccatcatggg cctcggtccc gtgcctgctt cgcagaaggc
     1801 attgtaccgc gccgggctta ctgtaaacga tatggacctg attgaattga acgaggcctt
     1861 tgcctcccag gccattgcct gcatccatga cctggacctc gatctgcaga aaataaatgt
     1921 gaacgggggc gccattgcac tgggccatcc gctgggttgc agcggggtgc gcatagccac
     1981 cactctcgtg catgagatga gaagaaggga atcgcattat ggaatggcgt ccatgtgcgt
     2041 gggagtagga cagggggctg ccgttgtttt tgaaaacgcc ggaagatgaa tctacttatg
     2101 ctgtgttatg cgcctggcgt atgacaggag attctcaaaa ccctgttctt cctggattcc
     2161 ggttgaaagg tctttgatct ttgccttgcc tgttttcagt tcttccgttc cgataatgac
     2221 cacaaagggt atacttttct tttcggcata tctgaactgc ttatcgaacc tggattcatc
     2281 cggaaataat tcgcagcgga tgccggcacc gcgaagctgc tccatgagtt caaagcatct
     2341 ggcgacggct tccggcccca ggttaaaaaa aagaaattcg gtggagcgct ttacggcttc
     2401 gggaaaaagt cttaactctt ccattgcgtc atagatccgg tccaccccga aggagatgcc
     2461 cactcccgga attccgggaa ctccgaaaag gccggtaaga tcatcgtatc tgccaccgcc
     2521 gccgatgctt ccgatgttca cttttccggc gcgcacctca aaaataatcc ccgtataata
     2581 attgagcccc cgggccaggg aaaaatccag cacgatattt tttgaacccg ggaaaaaatt
     2641 caggacctct tgaatttcgc gaatcccttc cgatgcggat ccgaccttgc ctttgtgtaa
     2701 cggatcacta cccggtgccg cgttcagtaa gttcccggcc tgcagcagtt tttcttcatt
     2761 ggttccctga ataccgaggt attgctgaac cagactgatc tgccggtcag aaagctttct
     2821 ttcccccagt tcctgcttca ctttatctat gccgatctta tccagtttgt caatcgccgt
     2881 gctgatggaa acgaggtttt cctgtccgcc gcaaagggcg gcgagcccgg ccaatatttt
     2941 gcggtggttg atgcggactt ccacctccag cttcaattct tcaaaaaccc ggaggtagat
     3001 ccgggtcagt tctgcttcat tgagcaggga gcggctgccg acaacatctg catcacactg
     3061 gtagaattca cggtagcggc ctttttgcgg gcggtccgcg cgccagaccg gctggagctg
     3121 gtaccgttta aaagggaagc tgagctcgtt atggtgcata gccacatagc gcgcaaaggg
     3181 gatggtcagg tcgtagcgta aggcgcgttc ggtgatgagc ggattatttt ttccggccag
     3241 gaccttttca aattcagcag cggccgaatc tttttttgag gaatgatcca gaccgttatt
     3301 caggatttta aacaggagct tgtcaccctc ctctccatat tttccgagca gggtttccag
     3361 gttttccagg gcgggcgttt ccaggggttc atatccgtac aactcaaaga cccgccggat
     3421 ggtattgaaa ataaattgtc gtttgtaaac ggtctccggt ccgaaatccc ttgttccctg
     3481 tggtaatccg ggcttgttca tgttggaatc ttaggcggtt ttaaggtggc cgtatttctg
     3541 tatgatccgg tcgcaggctt tgtccagcag atcataaaca atatgatagc ccggttcgtc
     3601 cccataccag ggatcgggca catcccggtt ttcccccgga tatacttcat tcagcagaag
     3661 atcagttttt gaaacattga atttctttcc cgaaatggct ttcatctccc ggagcacgtc
     3721 ggatgacatc gcatagatct tgtcaaaccg gtcaaagtct tccggaacaa aggacctggc
     3781 gcattgggtg ctgatatcca gttcgtgcag actggccact ttctgcgata acggatgggg
     3841 tgcttccccc acatgataac cgttgttggt gccggcgcta tccaccatcc agggaagtcc
     3901 ttcgtttttt gctttttttt ccaggatccc ttctgccagc ggactgcggc agatatttcc
     3961 caggcaaacc atcagaattt tcatggttgc aaagctaagg gaaaatgcct agtgggtaaa
     4021 gaacaagaac tgtttatgga ttccggcaac tttcgtgtct caatacgggt tctgcgcatt
     4081 cacccggtat ctgatttaat tcatccatta aaataaatag tatggatccc ctggccattc
     4141 tgtataccga cttcctggac cttttatttg agaacagaaa taaatcatat ggagcatatc
     4201 ccttgcgaaa gtattatccg aaaaggtttt tcattgcagt cggaattgtt ttcgggattg
     4261 ttttgatttt tgcttccgcg gtaatccttt ccggaaataa ggaattcatt tccagcagga
     4321 ttatgtacgg accggatccc cacctgacgc gggtcgtgct ggaaccccgg gtaataaacg
     4381 agccgcatgc cctgaagccg gcggcagccg gaaaacgcct ggcgccggtc agctctggtc
     4441 cgccggtgat tgtaaaggat caacacatac ccaggcccat agtaaccgga gaggaactga
     4501 atatggctcc ttccggcgca agcgctgagg cagggccgcc ggtaacaggc gggccggtga
     4561 aacagggggc tgcctcttcc gggggaatcg attctgcgca ggcaagaccg gaaattctgg
     4621 accgggcgca gatcatgccg gaatttcccg gcggactgga agcgctaagg cggtttttat
     4681 tgagaaatct gcggattccg gatagcgtga cggaaccggg cagcgaggtg cgtgtgctgg
     4741 tgaaatttgt ggtcggaacg gatggaaaag tgaccggaat ccagctggtc caatcgggcg
     4801 gaatggcttt cgatccggaa gttcagcggg ttatttccag gatgccggtc tggaagccgg
     4861 gtattcagaa tggaagaaaa gtatctgtgt attataatct tccggtgaat tttgtaattc
     4921 ctccggacaa ttaattttga attttttgac gcagcctccg cccgatatct acgtgttatt
     4981 atgtatattg ccttttgctt ttaaatgatt tgttaaatgg ccgacgacca ggacccggat
     5041 attcgctcga aaagatatat gctcagaagg tccatcatgg attacggtat gggagcgatc
     5101 atattctgtc tcggcatttt ttttctgata gcacctaaag tcggggtaat attcgggatc
     5161 gacgatctgt tccggtattt attcggcgga ctttgcatcc tgtatggatt attccgggtg
     5221 taccgggggt acaagaaaaa ttattttaac taaaattatt tggcatgaac atttggaaaa
     5281 gatgtatgcc tttaatacta ttggcattcc tggaactctc cttttccgga tgtggtcaca
     5341 ggaagcggat acctgtagaa accgccactt cgggaacgat tcatatcagc gtggatgagg
     5401 cctttaaacc cgtgatcgat tcacagctta aagtgtttga aagttctttt ccggacgcaa
     5461 aaattatagt ggagtataag ccggaagtga actgtttacg cgatctggaa aaagacagta
     5521 cgcggatgat catcgtcacc cggggcctca gcctcaaaga ggaagctttt tacagggatt
     5581 ccctccagcg gccggctatt tccggaatcc tggcctatga tgccgtggcg ctgatcgtga
     5641 acgcccattc cagggatacc gtgatgtcca tgccggagat ccggtcatta ctggaaggaa
     5701 atgataaaaa gggccgtcag ccggtaatgg atggtgtttc cgctaccagc acggtccggt
     5761 ttgcgatcga ttcgatcctg ggcggaaaac ccctgggaaa aaacgtggag gccgcccggt
     5821 ccagccccca ggtgatcgat tatgtggcat cacacgaaaa cgcggttgga tttatcggtg
     5881 tgagctggat cggggatgag gaagattctg tgcaactttc ttttctgcaa aaggtgaaag
     5941 tggttgccgt ggaatgtgcg ggatgtcctg aaaagcctta tgttcagcct tttcaggcta
     6001 atattgtgct gaaaaggtac cccatggtac gcggattgta ttatatacta aaggaaaatt
     6061 acagcggtat cggaaataat tttgtgaatt tcctgcagaa cgaacggggg caattgattt
     6121 tcagaagggc ttatctggtg ccggcgcgga tgagctttaa tgtccgggac gttcagctcc
     6181 aatagtaaat gattttttag ggatatattt ttacacatta aactgagatt tagaatgaga
     6241 aaactaagca tcagcctgtt actgtcggtt atctgcatcg gcagtacggt aacggcacaa
     6301 acggtagaac aggggaaaca gtttttatac tatgagcggt ataaaagcgc ccaggatgca
     6361 ttcgaaaaag ttttggcggc caacccaaat aatatcgatg cagtgtattg gcttggccaa
     6421 acgatgatcc atcggaggga tacccgggat acagcaggag caaaagcctt gtatcagaaa
     6481 atgctggcga ccaacggaga cgcccctttg ctgttagtcg gaatgggaca tgtggaatta
     6541 ctttatggaa acaccacgga ttcccgtcag cggtttgaaa gagcgattag cctgaccaaa
     6601 gaaaaagatg ttaatgtatt gaatgcggtt ggttacgcga atacggacgc caaagcagga
     6661 gatgtcaatt atgccattca gaaacttacg ctggccacac agacccggaa gttcaacaat
     6721 gccgaaacct atgttttgct gggggacgct taccggaaaa tgctcgacgg gggaaatgcc
     6781 gtgttaaatt ataataaggc gctcagcctc gatccgaaac tttccgaggc caaaatgaag
     6841 atttgtatca tctatctcac ccagaataac cgggattatt ttctgcccgc ttttgaagat
     6901 gcgatccgga ttgatcccgc ttatgctccg gcctatcagc aactgttcat ttactggtac
     6961 agccgcgacg tgaacaaggc gtctgcctac ctggacaaat acctggcaaa ttctgaccag
     7021 ggaccggaag cggaatacct gaaagcggat atgctctatg catccagcaa gtttgccgat
     7081 gcgcgtaccc gggcacagca gctgattacc cagaacgggg ataaagtgat cccccgcatg
     7141 taccgcatgg tcgcctatac ttccgatacc cttggcgatc tgccgaccgc gaaaacggcc
     7201 atggccaatt tcttcagtaa ggcagatact tctgccgaac gcggattgga ttacaagatc
     7261 cttccaacgg attatgaaga atacgcaaag atcttttcca aaatgccgga ttctgcttcc
     7321 caggtccagg ctttccagta ctataatatg gccattgcca tggataccct tccggcaaac
     7381 aaggaaaaat atgccaatgc cggactggaa ctggccaaaa agttaaaaaa cccgcaggca
     7441 gcggcaacac tttccgatgc gatgtataaa gccattaaag agccgaacca gtcggacctg
     7501 tataaatacg gaatggccaa ctattctgcc ggaaattata aaaaggccga cagtattttc
     7561 tgcggcgtct atgaatccaa atacccgacc gagatttatg gatacctctg gtgcgcaaaa
     7621 agtaaacagg cgcaggatga cagcacgaat tcccaggggc tggccgtgga ggcctatgat
     7681 aaactggcaa cgtttgccag gggaattgcg g
//
LOCUS       DASYCU010000293         7609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_208951, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000293 DASYCU010000000
VERSION     DASYCU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208951"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGM24_05930"
     CDS             complement(<1..347)
                     /locus_tag="VGM24_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEY4154941.1"
                     /translation="MTIHKEGYKTIAICSLVFAILNLVSFYFISFYEPWLSWAIFIVS
                     LIFLLFMISFFRVPFRVPTVNDKYVVCPADGKVVVIEEITDEEYFHDRRIQVSIFMSP
                     ANVHRNVNAVGGEV"
     gene            complement(369..1247)
                     /locus_tag="VGM24_05935"
     CDS             complement(369..1247)
                     /locus_tag="VGM24_05935"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY4154942.1"
                     /translation="MAIQAVHFRIRALTSLVFVAVMLFGLLWNQWSFFVLFSIIHFGC
                     WYEYQKLLERIDPAYREISAFQKYGVMVAGWCILMYCSKEGYATDDFSLHNVGLYGCI
                     IFLFILPVSEILYVRKPQLRNMGYSSLGLIYISLSLGFLLNLRTYYPAPVLDTVNKIL
                     VLMIILSLWVNDTMAYLVGSFFGKTPLSSISPKKTWEGTIGGVLFSVIVMGVVGYFLS
                     SGFELQAVSQWMIVAAIASITGTLGDLLQSKLKRAAGVKDSGQIMPGHGGFLDRFDSL
                     LFATPFIWLYIVMFAQ"
     gene            complement(1355..2323)
                     /locus_tag="VGM24_05940"
     CDS             complement(1355..2323)
                     /locus_tag="VGM24_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY4154943.1"
                     /translation="MNTYLRNKPAWMQLIIFGGLTGGILFLVMTIGVSLVAHANGMSM
                     MQIASMKPEDYARPELAGVVRGLLIVQFFGIFLLPSLIFAYLADPRPLAFAGLKAPDK
                     KSFILWGILIIVAAFFMVEWLSLINQELVSHLLGKSAKQWVEKGESETDNTLRNILAM
                     KNTRDLLQSVLLVGVMAAVGEELFFRGIMQRLLIQACKSPWAGIILTAAIFSAFHGQF
                     MGFIPRMILGIILGALYWYSGSIIPAMAGHFIYNTGQLLLVYFKLADMDSKSVPDTMT
                     SIVGIASLVLVIFLLNLLRKNSITTYERMYAVKERDMFEDDMNPKS"
     gene            2459..3484
                     /gene="dusB"
                     /locus_tag="VGM24_05945"
     CDS             2459..3484
                     /gene="dusB"
                     /locus_tag="VGM24_05945"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY4154944.1"
                     /translation="MAIQIGNIVLPDFPLLLAPMEDVSDPPFRFVCKRYGADLMYSEF
                     ISSEGLIRDAIKSRQKLDFFEYERPFGIQLFGGDEEAMGLSARIIDATNPDLVDINYG
                     CPVKKVVCKGAGAAVLKDINAMVRLTRAVKQSTRLPVTVKTRLGWDGQSQNIEEVAER
                     LQDEGIAALTIHGRTRAQLYKGEADWRLIARVKNNPRIRIPIFGNGDIDSPEKALEYK
                     NRYGVDGIMIGRAAIGYPWIFDEIKHFFKTGTRLDPPAIEQRVDAIRQHLYRSLEWKG
                     NVPGILEMRRHYSNYLRGFPYVREFRNQLVQKNSLEEIEVVLEEVVKRYSGVEQHAEM
                     HPSLVSG"
     gene            complement(3493..4014)
                     /locus_tag="VGM24_05950"
     CDS             complement(3493..4014)
                     /locus_tag="VGM24_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154945.1"
                     /translation="MLTRADIEKYFVAEKQESLLFLILGLAAVLLALIFYFGGKTLIL
                     RGAAVPLVVFGLIQAAVGYAVFQRSDDQRINNVYAYGMNPAQLKKGELARMGKVNRNF
                     KIYRLVEGGAVIAGIILVLAFYKNPDKHFWMGLGITLSVMAAELFIADFIAAKRALHY
                     TELLEKFEKSAEI"
     gene            4086..4895
                     /locus_tag="VGM24_05955"
     CDS             4086..4895
                     /locus_tag="VGM24_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY4154946.1"
                     /translation="MTRLLIAAFIPLLLTACKTNTVKVDDSLKAEFDSAGVTGCFALF
                     NNSQNDFTVYNLARYRDSAYTPASTFKIVNSLIGIQTGRISDENMVIKWDGVTRWNPD
                     WNRDLSMKQAFAVSSVPYYQEVARRIGHDTMQHWIDSLGYGNRDISGPVDSFWLNNRL
                     KVKPDEELGLVKKLYFNQLPFFHRTQEMVKKVMLREENTNYQLSYKTGWGNLENGNQL
                     GWMVGWIEENKHPYFFVLNFETPDKNMDIKAVRIQLLRRILSHFGFFNGKK"
     gene            4930..5934
                     /locus_tag="VGM24_05960"
     CDS             4930..5934
                     /locus_tag="VGM24_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY4154947.1"
                     /translation="MHFEHLEYLIALLVIPGLILLFNLLLSWKKKVGKRIGDPLLVKQ
                     LVSSFSARHFLIKFVLTLLVLSLLIAGMANLRSRAASESITRQGVDVMIVLDVSKSML
                     ARDIKPSRLDKAKQLLYRLVDKLQNDRLGLILFAGRAYMQMPLTPDHGAAKMYISEAG
                     PDAVPTQGTVISDALNMANNSFNRNEHKYKAVLLISDGEDHDPEGLKTAKQLAENGVM
                     INTVGIGSPDGSIIVDPATNEPKKDAKGNIVISKLNEQELQGIADATNGIYIRLDNLE
                     DANITLSQRLDGIEKRALTESEFINYKNYFPWFVGAALILLVIELYIPERKRTLRENA
                     "
     gene            5948..6607
                     /locus_tag="VGM24_05965"
     CDS             5948..6607
                     /locus_tag="VGM24_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4154948.1"
                     /translation="MLFLGSLLASVTGYAQPADALIRKGNRYYKAQDFDQSKANYEKA
                     LKQSPGNVNANFNLGNAEFRKNDFERAAAGYDEVLKGNTDTKTREDGWYNKGVAMIKQ
                     KKLDESIDAWKHALELNPEDQEARDNLVKALLEKKKQQQQNNSKDKKDKKPKPDKQNQ
                     QQKDQQKNQEDNRPKPQQSKLSKQQVEQYLRSLIQREKDVQDKMNQSKTHTPDQPDKD
                     W"
     gene            6652..>7609
                     /gene="ispG"
                     /locus_tag="VGM24_05970"
     CDS             6652..>7609
                     /gene="ispG"
                     /locus_tag="VGM24_05970"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HEY4154949.1"
                     /translation="MMQLYTPSLTRYERLKTVPVKVGSLIIGGDAPIRVQTMTTPDTM
                     DTKATVEQSIRCIEAGAELIRITAPSKTEAENLLNIKNEIRKRGYQTPLVADIHFTPN
                     AAEIAARIVEKVRVNPGNYVDKKKFQFIEYSDKEYSEEIERIHDRFLPLIRICKEYGT
                     AMRIGTNHGSLSDRIMSRYGDSPMGMVESAMEFLRIARDENYHQIVLSMKSSNPLVMV
                     QAYRLLVHNMQQEFGILYPLHIGVTEAGDGEDGRIKSAIGIGTLLEDGIGDTIRVSLT
                     EDPEFEIPVCQDIVARYTFRKADETVPPVEKIPYSPFEYKRRI"
BASE COUNT         2124 a         1909 c         1933 g         1643 t
ORIGIN      
        1 acttccccgc cgacggcatt gacattccgg tgcacattgg ccgggctcat gaatatcgag
       61 acctggatac gccggtcgtg aaaatattct tcatcggtga tctcttcgat gaccaccact
      121 ttaccgtcgg ccggacaaac aacatattta tcgttgaccg tcggaacccg gaacggaacc
      181 cggaaaaatg aaatcatgaa aagcaggaag atcaggctga cgataaagat cgcccagctc
      241 agccagggct cgtaaaaact gatgaaataa aaggagacca ggttcagtat cgcaaatacc
      301 aggctgcaga tggcaatggt tttatatcct tctttgtgaa tggtcatgaa taaatcgggt
      361 tgctgaaatt attgtgcaaa cataacaatg tatagccaaa tgaagggggt ggcaaaaagc
      421 agggaatcaa accggtccag gaacccgcca tgaccgggca tgatctgtcc gctgtccttg
      481 acgccggccg ctcttttcag cttgctttgc agcaggtctc ccagggtgcc ggtgatggag
      541 gcaatggccg caacgatcat ccattgggaa acagcctgta attcaaatcc gctgctcaga
      601 aaataaccga cgacacccat gacgatgacc gagaaaagaa cgcctccgat ggtgccttcc
      661 caggtttttt tcggagagat gctggagagc ggtgttttac cgaagaagga tccgacgagg
      721 taggccatcg tatcattcac ccataagctc aggatgatca tgaggacgag aattttattt
      781 accgtgtcca gaacaggcgc cggataatac gtacgaaggt tcagcaggaa ccctaaactg
      841 agagaaatat aaatgagccc cagcgaagag tagcccatat tccggagctg cggcttgcgc
      901 acataaagta tttccgaaac tggcaggata aaaagaaaaa tgatacagcc gtaaaggccc
      961 acgttgtgga gcgaaaaatc atcggtcgca tagccttcct tgctgcaata catcaggatg
     1021 caccatcccg caaccatcac cccgtacttc tgaaaagcgc tgatttcccg gtacgcaggg
     1081 tctatccgtt ccagcaattt ctggtactca taccagcatc cgaaatgaat gatggaaaaa
     1141 agcacaaaaa aactccactg attccaaagc aatccaaaca gcatcaccgc aacaaaaacc
     1201 agcgatgtca gcgccctgat ccggaaatgg actgcctgga tagccatgaa gtgaagatac
     1261 ggattacgga gaaaacgaaa aacgcgcaag atttttttaa gatcttactt accggaccat
     1321 tttgatccgc ccctccggtt taccgcaccc cggttcagga tttaggattc atatcgtcct
     1381 caaacatatc gcgttccttc accgcataca tccgctcata cgtggtgatg gaatttttcc
     1441 gaagcaggtt tagcaaaaaa ataaccagga ccagcgaagc aatcccgacg atgctggtca
     1501 tcgtatcggg aacgcttttc gaatccatat ccgcgagctt aaaatagacc aggagcaact
     1561 ggccggtatt gtaaataaaa tgcccggcca tagccggaat gatacttccg ctataccaat
     1621 ataaagcgcc gagaatgatg cccaggatca tacggggaat aaaccccatg aattgtccgt
     1681 gaaaagccga gaagatcgct gcggtgagaa taattcccgc ccaggggctt ttgcaggcct
     1741 ggatcagaag tctttgcatg atcccccgga aaaaaagctc ttccccgaca gcggccatta
     1801 cacccaccaa tagtaccgac tgtagcaggt cgcgggtatt tttcatggcc agaatattcc
     1861 ggagcgtatt atccgtttcc gattcgcctt tctccaccca ttgcttggcg gatttaccca
     1921 gcaggtggga aacgagctcc tggttgatca gggaaagcca ttccaccata aaaaaagcgg
     1981 cgacgataat caggataccc cataaaataa aggatttttt atcgggcgcc tttaacccgg
     2041 caaaggccag gggacgcgga tccgccaggt aggcaaagat cagcgagggg agcaggaaga
     2101 ttccaaaaaa ctgcacgatc agcaaaccgc ggactacgcc ggcaagttcc ggtctggcat
     2161 aatcttccgg tttcatggaa gcgatctgca tcatgctcat tccgttggca tgcgccacca
     2221 gcgatacgcc gatggtcatg accagaaaaa ggatgccgcc cgttaatcct ccgaaaatga
     2281 tcagctgcat ccaggcgggc ttgtttctca gataggtatt catgtaaggg cttccgtctt
     2341 ctgttttaat aaattatctt tgccatccgg aagtgctgcg aaattaagca agaccgcagc
     2401 aatattccgc ataacgggag agtaacccgg tattttagct ttctctcaaa ctttaatgat
     2461 ggcaatccag atcggaaaca tcgtattacc ggatttcccg cttttactgg cgcccatgga
     2521 ggatgtgagc gatccgccct tccgttttgt atgcaaacgg tatggcgccg acctgatgta
     2581 cagtgaattc atttccagcg aaggactgat ccgggatgcc atcaagagca ggcagaaact
     2641 tgattttttt gaatacgagc ggccttttgg catacagctt ttcggcgggg acgaagaagc
     2701 gatggggcta agcgcccgga tcatcgatgc gaccaacccc gacctggtgg atatcaacta
     2761 cggatgcccg gtgaagaaag tggtttgtaa gggcgccgga gcagcggttc ttaaagatat
     2821 caatgccatg gtgcgcctta cccgtgccgt gaaacaatcc acgcggctac ccgtaaccgt
     2881 taagacgcgg ctgggctggg acggtcagtc gcaaaatatc gaagaagtgg ccgagcggtt
     2941 gcaggacgag gggattgccg ccctgaccat tcacggaaga acccgcgcac aactatataa
     3001 aggggaggct gactggcgct tgatcgcccg ggtaaaaaac aatccccgga tccggattcc
     3061 gattttcgga aacggcgata ttgattcgcc tgaaaaagcg ctcgaatata aaaaccggta
     3121 tggggtggac ggcattatga tcggccgggc cgccatcggt tatccctgga tctttgacga
     3181 aataaaacac ttctttaaaa ccggcacccg gctggatcct ccggccatcg aacaaagagt
     3241 ggacgccatc cggcaacacc tgtaccgctc cctggaatgg aagggtaatg tacccggcat
     3301 cctggaaatg cgccggcatt acagcaatta tctccggggc ttcccctatg tacgggaatt
     3361 ccggaatcaa ctggtgcaga aaaattccct ggaagaaatt gaagtagtgc tggaggaggt
     3421 ggtgaagcgt tattccggcg ttgaacagca tgcggaaatg cacccgtccc tggtcagcgg
     3481 ttgaggaaga tttcagattt cagcgctttt ttcaaatttt tccaatagct ccgtatagtg
     3541 cagtgccctc ttagcggcaa taaaatcggc aataaataat tcggcggcca taacggaaag
     3601 cgtaatgccc agacccatcc agaagtgttt atcgggattt ttgtaaaatg ccaggaccag
     3661 gataatgccg gcgattacgg caccgccttc caccaggcga tagattttga agttccggtt
     3721 cacttttccc attcttgcca gctctccttt ttttaactgg gcggggttca tgccataggc
     3781 gtatacgttg ttgatccgct ggtcgtcgct gcgctggaac acggcgtacc ctacggcagc
     3841 ctggatcagt ccgaatacga ccaggggtac ggctgcgccc cggagtatca gggtcttccc
     3901 tccaaaatag aaaatcagcg ccagcaaaac ggcggcaagc ccgagaataa ggaaaagcag
     3961 gctctcctgc ttttccgcaa caaaatattt ctcgatgtcc gcccgggtaa gcatataaaa
     4021 gattctcatt aaatatacta atatcccgtt cgttcaaccc ttcttatttt tgccaaaatt
     4081 ctaccatgac ccggctcctg atcgctgcat tcatcccact attgctgacc gcctgcaaaa
     4141 ccaataccgt gaaagtggac gacagcctga aggcggagtt cgattcggcg ggcgtgaccg
     4201 gctgttttgc cctgtttaac aattcccaga atgactttac ggtttacaat ctcgcccgct
     4261 accgggacag cgcgtatact ccggcctcca cctttaagat cgtgaattcc ctgatcggca
     4321 ttcagaccgg ccggatcagc gatgagaaca tggtgatcaa atgggatggg gtgacccgct
     4381 ggaatccgga ctggaacagg gacctcagca tgaaacaagc ctttgccgtt tcctccgtgc
     4441 cttattatca ggaagtggcg cggcggatcg gccatgatac catgcagcac tggattgatt
     4501 ccctgggtta tggcaaccgg gacatcagcg gtcccgtgga ttccttctgg ctgaacaacc
     4561 ggctgaaggt gaagcccgat gaggaactcg ggctggtcaa aaaactatat ttcaatcagc
     4621 ttcctttttt tcaccgtacc caggaaatgg tgaagaaagt gatgctgcgg gaagagaaca
     4681 caaactacca attaagttac aagaccggct ggggcaatct ggaaaatggg aaccagctcg
     4741 gctggatggt gggctggata gaagagaaca aacaccccta tttttttgta ctgaactttg
     4801 aaacgccaga taagaacatg gacattaaag cggtccgaat tcaattactc aggcggatcc
     4861 tctcccattt tggatttttt aacggaaaga aatgaggttg gcggagggcc ttcgttaact
     4921 tgcaatcata tgcattttga acatttggaa tacctgatcg ctctgctggt gattccgggt
     4981 ctgatccttc tttttaacct tttgttaagc tggaaaaaga aggtcggcaa acggatcggg
     5041 gaccccctgc tggtaaaaca actggtaagc agtttttccg ccaggcattt tctgatcaaa
     5101 ttcgtgctga ccctgctggt cctttcgctg ctgatcgccg ggatggctaa tctgagatcc
     5161 agagcggctt ctgaaagtat cacgagacag ggcgtggatg taatgatcgt actggacgta
     5221 agcaaaagca tgctggcccg ggatataaaa cccagccgcc tggacaaggc caaacagttg
     5281 ctataccggc tggtggacaa acttcagaat gaccggctgg gcctcatcct ttttgccggg
     5341 agagcttata tgcagatgcc cctgacgccc gatcacgggg ctgcaaaaat gtatatttcc
     5401 gaagccggtc cggacgccgt tcccacgcag ggaacggtca tttcagacgc ccttaatatg
     5461 gcgaacaatt catttaaccg aaatgaacac aagtataagg ccgtgctgtt gatctccgac
     5521 ggggaagacc atgatccgga aggtttgaag accgcaaagc agctggctga aaacggggtc
     5581 atgatcaaca cggtcggaat cggttctccg gatggttcga tcatcgttga tccggcaaca
     5641 aacgaaccga agaaagatgc aaagggaaat attgtcatct ccaaactgaa cgaacaggaa
     5701 cttcagggaa tcgccgatgc gaccaatggc atttatatcc ggctggataa cctggaagat
     5761 gccaatataa ccctgtccca gcggctggat gggatcgaga aacgggccct gacggaaagt
     5821 gaattcatca attacaaaaa ttatttcccc tggtttgtcg gggctgccct gatcctgctg
     5881 gtcattgaat tatatattcc ggaacgaaaa agaaccctcc gtgagaacgc ttaagtcaat
     5941 acgaaatatg ctgttcctgg gctccctgct ggcttcggtc acgggctatg cccagccggc
     6001 ggatgcattg atccggaaag gaaaccggta ttacaaggca caggattttg atcagtcgaa
     6061 ggccaattat gaaaaggcgc tgaagcaatc tcccgggaat gtgaatgcca attttaatct
     6121 cggcaacgcc gaattccgga aaaatgattt cgaacgggcc gccgccggtt atgatgaagt
     6181 gctgaaaggg aataccgata cgaaaacccg ggaggacggc tggtataaca aaggcgtggc
     6241 gatgatcaaa cagaaaaaac tggatgaaag catcgacgcc tggaaacatg ccctggaatt
     6301 gaatccggaa gaccaggaag cacgggataa cctggtgaag gcccttctcg aaaaaaagaa
     6361 acagcagcag cagaacaaca gtaaggacaa aaaggataaa aagccaaagc cggataaaca
     6421 gaaccagcag caaaaagacc agcaaaaaaa tcaggaagac aaccggccca aaccccagca
     6481 gagtaaactg agcaagcaac aggtggaaca atacctgcgt tcgctcattc aaagggaaaa
     6541 ggacgttcag gataaaatga accagagtaa gacccatacc ccggatcagc cggataagga
     6601 ttggtaaacc gccgcccgaa tcattaattt tgctattatt cttcaaggtc catgatgcaa
     6661 ttgtataccc cttcgcttac ccgctatgaa cggctgaaaa ccgtacccgt aaaagtgggc
     6721 agcctgatca tcgggggcga tgccccgatc cgggtacaga ccatgaccac gccggacacc
     6781 atggatacga aagctaccgt ggagcaatcc atccgctgca ttgaagcggg cgccgagctc
     6841 atccgcatca cggccccgag taaaacggag gccgagaacc tgctgaacat caaaaatgaa
     6901 atcaggaaaa gaggttacca gaccccgctt gtcgccgata ttcactttac tccaaacgcc
     6961 gccgagatcg ctgcccgcat cgtggaaaaa gtgcgggtga acccgggcaa ctatgtcgat
     7021 aagaaaaaat ttcagtttat tgaatacagc gataaggaat attcggaaga gatcgaacgc
     7081 atacatgacc gcttccttcc gctgatccgg atctgtaagg aatacggaac cgccatgcgg
     7141 attggcacca atcatggttc actgagcgac cggatcatga gccgctacgg cgattcaccc
     7201 atgggcatgg tggaaagcgc catggagttt ctccgcatcg cccgggatga aaactatcac
     7261 cagattgtac tgagtatgaa atccagtaat ccgctcgtga tggttcaggc ctatcgcctg
     7321 ctcgtgcaca atatgcagca ggaattcggc atcctttatc ctctgcatat cggggtaacg
     7381 gaagccggcg acggagaaga cgggaggata aaatcggcga tcggcatcgg caccctgctg
     7441 gaagacggta tcggagatac catccgcgtg tcgctgacgg aagatccgga atttgaaatc
     7501 ccggtttgcc aggatatcgt tgcccggtat accttccgga aagcggacga aaccgttccg
     7561 ccggtggaaa agattcctta ttccccgttc gaatacaaaa gaagaataa
//
LOCUS       DASYCU010000294         5177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4268995, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000294 DASYCU010000000
VERSION     DASYCU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4268995"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VGM24_05975"
     CDS             <1..244
                     /locus_tag="VGM24_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154950.1"
                     /translation="NFVDPALFAAHLQAGDAQIATAMANKSPLQGIPGSNYNPGDKRG
                     DPSQKDAYFTFGLKLGIRLGTDHSYDNSTRCPLLRF"
     gene            282..680
                     /locus_tag="VGM24_05980"
     CDS             282..680
                     /locus_tag="VGM24_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154951.1"
                     /translation="MKTVPRRKGSVRWRPAMMSAAFLIATGLSYAGSFPSSDPTAVVI
                     TKNLNSRKHKIRLFTASDSKTLLFTVDGVAGKHYTLFVFDLDGKLIAQSAILNHETGI
                     LPLIHSGGYLYDVFVEDHKVESGQLTVKQD"
     gene            complement(890..1288)
                     /locus_tag="VGM24_05985"
     CDS             complement(890..1288)
                     /locus_tag="VGM24_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154952.1"
                     /translation="MLQEVGLGYITLGQSAVTLSGGEAQRVKLGTELSKRETGKTFYI
                     LDEPTTGLHFEDISHLLNVLNKLVDRGNSVLVIEHNMDVIKVADHVIDLGPEGGDGGG
                     RILFEGTPEKLIDVKESFTGKYLKAEMKAG"
     gene            complement(1295..3751)
                     /gene="uvrA"
                     /locus_tag="VGM24_05990"
     CDS             complement(1295..3751)
                     /gene="uvrA"
                     /locus_tag="VGM24_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY4154953.1"
                     /translation="MIEPNSPSAPAGEEVIEVYGARVHNLKNIDISIPKNKLVVITGI
                     SGSGKSSLAFDTIYAEGQRRYMESFGAYARQFIGDMERPDVDKITGLSPVISIEQKTT
                     NRNPRSTVGTVTEVYDFLRLLYARIGEAYSYNTGKKMVKFSEEEIVENIFQKFKNKKI
                     SVLAPIVRGRKGHYRELFEDIRKKGFVKVRVDGEVKDLAPKMQVDRYRIHDIEVVVDR
                     LAVTPDMKTRVSQSTQNALKMGKDLMFLLINESNQVAQYSKHLMCEDTGISYEEPSPN
                     SFSFNSPYGACPQCKGLGAVYRVSMNAIIPDETKSVNEGGIVPLGGEREAYVFNQVQK
                     LAKKNKFNLDTPIRDMPPRALNLILHGTAESPGDNTLDFDENEATAGGVEYEGLVPML
                     KRWFGASGTDSLREWVERFMELKTCDACGGARLKKESLWFRVDNKNIAELNELNLDKL
                     YAWFSGIEKRLSPKQQLIAKDVLKEIRERLQFLLDVGLNYLTLNRPTRTLSGGESQRI
                     RLATQIGSQLQGITYILDEPSIGLHQRDNHRLIRALQNLRNIGNSILVVEHDKEIMMA
                     ADYLVDIGPNAGQHGGRVVAQGTPGSMLKLNTLTAGYLNGRVRIPIPAERRKGNGKLL
                     ELKGAKGNNLKNVDVSLPLGKFIVVTGVSGSGKSTLINETLYPVLSHHAFGSKGVALE
                     YKTIKGLEHIDKVIEIDQSPIGRTPRSNPATYCGFFTDIRTLFASIPEARIRGYNAGR
                     FSFNVKSGRCDVCEGGGMRVIEMNFLPDVYVHCEKCNGKRYNRETLEIRYKGKSISDV
                     LDMTVDEAVEFFQNVHYLYR"
     regulatory      3943..4054
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4247..>5177
                     /locus_tag="VGM24_05995"
     CDS             4247..>5177
                     /locus_tag="VGM24_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY4154954.1"
                     /translation="MQEITQFIHSIPVDPLTGAVSVPIYQTSTFVQEAPGVNKGYDYA
                     RSNNPTRATLESLIAGLEKGATGLAFASGLAAIDAVLKLLQSGDEVIAVDDIYGGAFR
                     LFEEVYKKFGVKLTYVDASDPANVFRVLSARTRLIWIETPSNPTLKIVDIGAIARVAK
                     AQNCLLCVDNTFASPALQRPIELGADIVVHSATKYLGGHSDLIAGLVVTASPELGEKI
                     RFFQNATGAILGPFDSWLVIRGLETLHLRMKQHGHSAQQIAEFLLDHPAVERVFYPGL
                     PTHANHAIAKRQSRGFGGIVSFTLKEDTLAAAKF"
BASE COUNT         1179 a         1446 c         1214 g         1338 t
ORIGIN      
        1 gaattttgtg gatccggcct tattcgctgc acatcttcag gccggggatg cccaaatcgc
       61 aaccgccatg gcaaacaaaa gtcctttgca ggggattccg ggcagcaact ataacccggg
      121 agataaaaga ggagaccctt cccagaaaga cgcctacttt acgtttggcc tcaaactggg
      181 cattcgcctc ggaaccgacc attcctatga caattcaaca agatgcccat tgttgagatt
      241 ctaaatagat tttgttaatc cgtacccttt atttttttct aatgaaaacc gtaccccgaa
      301 gaaaaggatc tgtccgttgg cgtcccgcga tgatgtcagc cgcttttctt atcgcaaccg
      361 gtctgtcgta tgcagggagt tttccctcct ccgatccaac ggctgtggtc atcaccaaaa
      421 atctgaatag cagaaagcac aagatcaggc tttttaccgc atcagattcc aaaacgcttt
      481 tatttaccgt tgatggggtg gctggaaagc attacaccct cttcgtgttc gacctggacg
      541 gaaaactgat cgcccagtca gccatcctga atcacgaaac cggcatcctt ccgttgattc
      601 attcaggcgg ctatctgtac gatgtctttg tggaagacca caaagtggaa agcggacaac
      661 tgaccgtcaa acaagattaa acgcaaacca ataagcggcg ggaaaattgg taagatccgt
      721 atatccgaag gttaagtaga acctgatccg ttccctattt tgtacccgac gattttatta
      781 attcaatccc gcttacctta ttggccttat accttttttg gttcaccaat atcctcaacg
      841 gccggcgaca aataattgcc gtccgtttgt ttttttaacg ggaggaagct tacccggcct
      901 tcatttccgc cttcaggtat ttcccggtga aactttcctt cacgtcaatc agtttttccg
      961 gagttccttc aaacaggatc cttcccccgc catcccctcc ttccggcccc aggtcaatca
     1021 catgatcagc taccttgatc acgtccatat tatgctcgat cacgagcacc gaatttcccc
     1081 ggtccaccag tttattcaat acattcagca aatggctgat gtcttcaaag tgcaacccgg
     1141 tggtcggttc atcgagtata taaaatgttt tgcctgtttc ccgtttggac aattcggttc
     1201 ctagctttac gcgctgggct tcgcctccgc tgagggtgac cgccgactgc ccgagcgtaa
     1261 tatatccgag ccccacttcc tggagcactt ttatctagcg gtacagataa tgcacgttct
     1321 gaaaaaattc aacggcttca tccaccgtca tatccagcac atcactgatc gatttccctt
     1381 tgtagcggat ttcaagggtt tcccggttat atcttttacc attgcatttt tcacaatgca
     1441 catacacatc cggtaaaaaa ttcatttcaa tcacccgcat gcccccgccc tcacacacat
     1501 cgcagcggcc gctttttaca ttgaaggaga accgcccggc attgtatccc cggatacggg
     1561 cttcgggaat ggacgcaaag agcgtacgga tatcggtaaa aaacccgcaa taggttgccg
     1621 gattgctccg gggcgtgcgg ccaatgggcg actgatcgat ctcgatcact ttatcaatat
     1681 gctccaatcc ctttatggtt ttgtattcca gcgccactcc ctttgatccg aacgcgtgat
     1741 gactcaatac cgggtataag gtctcgttga tgagggtgga tttgccgctc ccgctcacgc
     1801 cggttaccac aatgaatttt ccgagcggca ggctcacgtc cacatttttt aagttatttc
     1861 cttttgcccc tttcaattca agcagtttcc catttccttt tcgccgctct gccgggatgg
     1921 gaatccggac acggccattc aggtaaccgg cggtaagggt attcagtttc agcatagatc
     1981 cgggtgttcc ctgcgccacc acgcgacctc catgctgacc ggcattcggc ccgatatcca
     2041 ccaggtaatc tgcggccatc atgatctctt tatcgtgctc cacgaccaaa atactattgc
     2101 cgatattgcg cagattctgc agggcccgga tcagccggtg attatcgcgc tgatgcaaac
     2161 caatgctcgg ttcatccagt atataggtga tcccctgtaa ctgcgatccg atctgtgtgg
     2221 ccagccggat acgctgggac tcgccgccgc tcagggtccg cgtcggacgg ttcagggtaa
     2281 ggtaattcaa ccctacgtcc agcaggaact gcaggcgctc ccggatctcc ttcagtacat
     2341 ctttcgcaat caactgctgc tttggactca atcttttttc aatcccgctg aaccaggcat
     2401 ataacttatc caggttgagc tcattcagct ccgcaatatt tttgttgtct acccggaacc
     2461 acaaactctc tttcttcagt ctcgctccgc cgcaggcatc acaggttttc agttccatga
     2521 accgctccac ccattcccgc agggagtctg ttcccgatgc accgaaccat cttttaagca
     2581 tgggcaccag cccttcatat tccacgccgc ctgccgtcgc ttcgttttcg tcaaaatcca
     2641 gggtattatc cccgggagac tctgctgttc cgtgaaggat caggttcaat gccctcggag
     2701 gcatgtcccg gatgggggtg tccagattga atttattttt cttcgccagt ttctgcacct
     2761 ggttaaatac ataggcttcc cgttcgccgc ccagcggaac aatgccgcct tcgttcaccg
     2821 atttagtttc atccggaatg atcgcgttca tgcttacccg gtaaacagca ccgagcccct
     2881 tacactgggg gcaggcgccg taaggtgaat tgaaggaaaa gctattggga gaaggctctt
     2941 cgtaactgat gccggtgtcc tcacacatca ggtgcttgct gtattgcgct acctggttgc
     3001 tctcattgat caacaggaac atcaggtcct tccccatttt gagggcattc tgggtactct
     3061 ggctcacgcg tgttttcatg tcgggagtaa cggcaaggcg gtccaccacc acttcaatat
     3121 catgaatcct gtaccggtcc acctgcattt tcggggccag gtcctttact tctccatcca
     3181 cccgcacttt cacaaaaccc tttttgcgga tatcctcaaa cagttcccgg tagtgtcctt
     3241 tccgcccgcg cacaatgggg gccagcaccg atatcttttt atttttgaat ttctgaaaga
     3301 tattctctac gatctcttcc tcactgaact tcaccatctt tttgcccgta ttataggaat
     3361 aggcttcccc gatgcgggca tagagcagcc gcaggaaatc gtacacttcc gtaaccgttc
     3421 ccacggtgct ccgggggttg cggttagtgg tcttttgttc aatggagatc accgggctca
     3481 ggccggtgat cttgtccaca tcgggccgct ccatgtcccc gatgaactgc cgcgcatagg
     3541 cgccaaaact ttccatatac cggcgctggc cttcggcgta aatggtatca aaggccagcg
     3601 aggatttccc gctgccgctg atgccggtaa tgaccaccag cttattcttg ggaatggaaa
     3661 tatcaatatt cttcagatta tgcacccggg cgccatacac ttcaatcact tcttcgccgg
     3721 ccggtgcaga cggactattc ggttcaatca taaataaaaa tggaatcgat aaagttaaca
     3781 acaaaatgcg ggttggcggg gctctgaaaa tccgggaata cccaatcatt aaacaaaatc
     3841 cggaggtaaa tgttccgggg gcgggtatat ttctgaaccc cgggataaaa taattttttg
     3901 gaatcaatgg ctgtttttat ctttgctcca gttcttatca aactcttatc aagaaaggct
     3961 gagggaaatg gcccgctgaa gccttgacaa cctgcccgga tactatccgt gcaaggtgcc
     4021 aactccatct cgtccaggac gggacagata agtcagattc aaagcttttt aatcgatcaa
     4081 catattaaaa gctcttctga cggttcggaa gagctttttt attgcctgcc ggtgaacaac
     4141 cactccagca tccatagaag ggcttccggg gattccagct ttcttgcggg agcattcatt
     4201 cactctaaaa cgactcccgg cgagggagca aacaggagga ttttttatgc aggagattac
     4261 ccagttcatt cacagcattc cggtggatcc gctaaccggg gcggtttccg ttccgattta
     4321 ccagacttcc acttttgtcc aggaagcgcc cggtgtaaat aagggctatg actacgcaag
     4381 gagtaacaat cccaccaggg ccactttgga aagcctgatc gccggcctgg aaaagggcgc
     4441 cactgggctt gcttttgcca gcgggctggc cgctattgac gcggtgctca aattgctgca
     4501 aagcggggac gaggtgatag ccgtggacga tatctatggc ggggccttcc ggctctttga
     4561 agaggtttac aaaaaattcg gggtgaagct gacctatgtg gatgcctccg atccggccaa
     4621 cgtttttcgg gtgctcagcg cgaggacccg cctgatctgg atcgaaacac ccagtaaccc
     4681 caccttaaag atcgtcgata tcggggccat tgcccgcgtt gccaaagcgc agaattgcct
     4741 gctatgcgtt gacaatacct ttgcgtcccc ggcactgcag cggcccattg aactgggagc
     4801 cgatatcgtg gttcattcgg ccaccaaata cctggggggt cacagcgacc tgattgccgg
     4861 cctcgtggtg acggccagtc cggagctggg cgaaaagatc aggttctttc aaaacgccac
     4921 cggcgccatc ctggggccct tcgacagctg gctggtgatc cgggggctgg aaaccctgca
     4981 tctgcggatg aaacagcatg gtcattctgc ccagcagatc gcagaatttt tattggacca
     5041 tccggcagtg gaacgcgttt tctaccccgg cctgcccacg catgccaatc atgccattgc
     5101 caaaaggcag tcccgcgggt tcggcggcat cgtgtctttt actttgaaag aagatacgct
     5161 ggcggctgcg aaatttc
//
LOCUS       DASYCU010000295         7566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1514646, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000295 DASYCU010000000
VERSION     DASYCU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514646"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VGM24_06000"
     CDS             complement(<1..506)
                     /locus_tag="VGM24_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY4154955.1"
                     /translation="MVQIRISFLSVFMTGILCLPAKSQKKEDSFIPYDQGIPNTSVSF
                     KMIPIPAGSFLMGSPDKENGHRADEGPVTKVFIDSFWIEEHEVTYDEYILFADEERDQ
                     EPKPDGITRPSPPYIDFTQGMGKSGGFPANSMSQYGAVMYCKWLYRKTGIFYRLPTEA
                     EWEYACRAG"
     gene            complement(509..1429)
                     /locus_tag="VGM24_06005"
     CDS             complement(509..1429)
                     /locus_tag="VGM24_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY4154956.1"
                     /translation="MGSPFTITLYSNDSAAASRSAGAAFKRAGGLNEVLSDYIDSSEV
                     NRLSLTSGQGRYVPVSEDLFTILQRAEEAGRLSDGSYDVSVGPLVKLWRSARKTKIFP
                     SPDSIRAARKKTGYRYIHLDAATRSVKLEKRGMKLDLGGLGKGFVAAAALAELNRNGI
                     YCAMVNAGGKIVTGQAPPGKEGWLIGINAPGEKEEILPVLLNLKEISVATSGDIYQYV
                     EFNGKRYSHIVDPKTGIGLTRRRNVTAIAPDGTTADWLATACSILSPRKSLRLIKKIP
                     GAALLITERRKGGIRARSSTNFKKFLNKAL"
     gene            complement(1739..2959)
                     /locus_tag="VGM24_06010"
     CDS             complement(1739..2959)
                     /locus_tag="VGM24_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEY4154957.1"
                     /translation="MKIASILFILLLQTFPQPLPAQSSKDESGLAERNYLIRSLTRIA
                     DPVLGALDKNELKQKMPVEAKETDRRNYTYLEAFGRLLAGMAPWLELGADPTPEGQLR
                     KKYIELARAGLHNATDPHAPDFMNFNEGSQPLVDAAFLAQALLRAPHQLWEPLDGQTK
                     AHIIAALKSSRVITPGYSNWLLFSAVIEAALLKFGQDGDRMRMDYAIRQHQLWYKGDG
                     TYGDGPHFHWDYYNSYVIQPMLLETLKTLSEADKKQQGNYDQALRRAQRYAAVQERLI
                     SPEGTYPPIGRSLAYRFGTFQLLSKIALLKVLPDEIKPQQVRAALYTVIKKQMEAPGT
                     FDAEGWLQIGLYGHQPGIGEGYISTGSLYLCSEAFLVLGLPASDPFWLGPDEDWTSRK
                     LWKGEDAPTDHAAD"
     gene            3117..3632
                     /locus_tag="VGM24_06015"
     CDS             3117..3632
                     /locus_tag="VGM24_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4154958.1"
                     /translation="MEYHGEQDPASRTDNSLNRRVEISLRLPERQVARADSDQLSIRK
                     TYTLSKLYFRPDEAILEPSSMPYLDNVANILKTYTSGLFEIRGLVNWEAPPVASADSG
                     YKKKMDELSTARARLVYEILIDKGIPPERMRYKGMGNSQMLYPYASTDEEKRKNMRVE
                     ILILTKQKQTH"
     gene            3638..4738
                     /locus_tag="VGM24_06020"
     CDS             3638..4738
                     /locus_tag="VGM24_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEY4154959.1"
                     /translation="MKKISGLTILVFLSGCAGKSDPFPELSSFHKPVNYPILKGDPSI
                     TFFDSLKMENVAWKRADVFNPAAIVKDNKIYLLSRCEDNPAAILGGRTSRIGLSVSDD
                     GIHFSSLGSPVLYPANDAFKEYDYPGGCEDPRAVETEDGLYVMAYTSWNYKVPRLSIA
                     VSKDLLHWSKKGPAFAKAYQGRFLDLATKSGSMLTIMKNNRPVLAKRNGKYWMYWGEH
                     FVNLAWSVNLSDWYPLLDSNNNLKKTIVTRPGKFDSDLDECGPPALITGKGILLIYNG
                     KNSAGRKADPSLPEGTYSTGSVLFSLKDMETVIDRPDHPFLKPSLPHELTGQYTAGTT
                     FAEGLVYFKEKWFLYYGTADSFVGVAVTGNKD"
     gene            4820..5119
                     /locus_tag="VGM24_06025"
     CDS             4820..5119
                     /locus_tag="VGM24_06025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4154960.1"
                     /translation="MEDVKLKLNEKGRGSFYIAVGDEQLAMMEIGISENTLTAYHTEV
                     SPKAGGKGLGKELLIAMAAYAREHKYKVIPLCPFVNAQFKRHPEEYSDIWLKPVP"
     gene            5149..5790
                     /locus_tag="VGM24_06030"
     CDS             5149..5790
                     /locus_tag="VGM24_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4154961.1"
                     /translation="MSADHLQIKNIVSAGKNIQEAGRVLIMMHGRGATAESILSLAGQ
                     LRVNDFALIAPQALNRTWYPYSFMATPAQNEPALSASLSRLKELLNQLNGQGISSKNI
                     YFLGFSQGASLILEFACRNATRYGGIVAFTGGQAGDQIYSENYTGNFDGTPVFLGTSD
                     PDQHVPVIRVNETATILQKMNAAVTKKLYPYLGHTINSDEMDSANRLIFDPET"
     gene            5914..7371
                     /gene="cls"
                     /locus_tag="VGM24_06035"
     CDS             5914..7371
                     /gene="cls"
                     /locus_tag="VGM24_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEY4154962.1"
                     /translation="MNWLLFSEILYTLVLMMVCLRIITDTRTTNKALSYLLFAVFVPF
                     FGMIFYFLFGINYRKRKIYSKKLYENDALANKMRGDIYQYSKQTFNECDASVQSSREL
                     AYMLLKDTTSPLTGNNSVKLLMNGENKFPEVIRALKEAKHHIHVEYYIYEDDEIGRTI
                     ETILIQKAAEGVEVRFIYDDFGSRSIRRKLVPRLKANGVHAYSFFRIYFIPFANRLNY
                     RNHRKIIVVDGRTAFVGGINVSDRYINSPEDTKKLFWRDTHLRIDGPGVQYLQYLFLC
                     DWNFCTEKKLQPDSHFFPPLNSYPVKDDKVVQIAAGGPDSEMPTILFSLLKAVNLATK
                     EILISTPYFIPGDGLIEALMIASLGGIAVKLLVPGQSDSLLVNSAAKSYYKDLLQTGV
                     EIYLYKRGFLHAKTLVADRKIAIVGTANMDFRSFELNFEVNAIVYDMEIANQLSDVFY
                     ADLKDAEKIDAVAWGKRAKYKRLLEKTARLVSPLL"
BASE COUNT         1972 a         1929 c         1791 g         1874 t
ORIGIN      
        1 cctgcgcggc aggcatattc ccattccgct tcggtcggca ggcggtaaaa aatgcccgtt
       61 ttcctataga gccatttgca gtacatgacc gccccgtact ggctcatgct gttggcggga
      121 aaaccaccgc ttttccccat cccctgggta aaatcaatgt acggcggact gggtctggtt
      181 atcccatccg gtttcggctc ctggtcccgc tcctcatccg caaacaaaat atactcgtca
      241 taggtcacct catgctcttc aatccagaat gaatctataa aaactttcgt aaccggaccc
      301 tcatccgccc ggtgcccgtt ttctttatcc ggacttccca tcagaaagga accggccgga
      361 atgggaatca ttttaaagga aacgcttgta ttcggaatgc cctgatcgta aggaataaag
      421 gaatcttcct tcttctggct ttttgccggc aggcacagga tgcccgtcat gaaaacggag
      481 agaaagctga tccggatttg aaccattttc aaagcgcttt attcagaaat tttttaaaat
      541 tagttgagct cctcgcccgt attccaccct ttcttctttc cgtaatgagc agtgccgccc
      601 ccggaatctt ttttatcaat ctcaatgatt tgcgggggga aagaatactg caggcagtgg
      661 ccagccaatc ggcggtagta ccatccgggg caatggcggt cacattcctc ctgcgggtca
      721 ggccgatgcc tgttttaggg tccacaatat gtgaatagcg tttcccattg aattcaacat
      781 actgatagat atcgcctgag gtggcaaccg aaatttcttt cagattcaac agaacaggca
      841 aaatctcctc cttctccccc ggtgcgttta ttccaatcag ccagccttct tttcccggag
      901 gcgcctggcc ggttactatt tttccaccgg catttaccat ggcgcaatat attccgttcc
      961 ggttcagttc ggccagggcg gcggcggcaa caaacccctt gcccagtccg ccgaggtcca
     1021 gtttcattcc tcttttttcc agcttaacgg aacgggttgc tgcatccaga tgaatatacc
     1081 ggtaaccggt ctttttccgg gcagcccgaa tggaatcggg gctgggaaag attttcgttt
     1141 tccgggcaga ccgccataat ttcaccagcg ggcccacgct gacgtcataa ctcccatcac
     1201 ttaaccggcc ggcttcctcc gcccgctgaa gaatagtaaa cagatcctcc gatacgggca
     1261 cataccggcc ctgtccgctg gttaaactta ggcggtttac ttcactgctg tcaatataat
     1321 cactcagaac ctcgttcaac ccgccggctc ttttaaaagc ggcaccggcc gaacgggagg
     1381 ctgcagcaga atcattgcta taaagcgtga tcgtaaacgg agatcccatt tcgggttgtt
     1441 caaatatgta ttttttttga ccgctgcctg tttggacagc cagtaatatc aagccagtca
     1501 ataaatgctc ttttattgta aacagattta tgtttaatcc ggtaatggga tccctgtctt
     1561 atcattcctg tccggatcaa tattgtacca atgcctgaat gtactccatt ctgctgaatt
     1621 tccggaaggc gcatttaaat tatcaacgga atctcctccg ggattttgcg ggaaaaagaa
     1681 ttcaggtcaa attgacattt aataagcgga tgcggccggc tggtgatcag gggcatcctt
     1741 agtctgctgc atgatcggtt ggggcgtctt ctcccttcca taatttcctg gatgtccagt
     1801 cttcgtccgg acccagccag aatgggtcag atgccggcaa cccaagtacg aggaaagctt
     1861 cagaacacag ataaaggctt ccggttgaaa tatacccttc cccgatacct ggctggtggc
     1921 catacaaacc aatttgcagc cagccctccg catcaaaagt tccgggcgct tccatctgct
     1981 ttttgatgac tgtatataat gcagccctta cctgttgtgg tttaatttca tccggcagaa
     2041 ctttcaataa ggcgatcttg gacaggagct gaaatgtgcc aaaccggtaa gcaagggaac
     2101 ggccgatggg cggataagtg ccttccggtg aaatgagccg ctcctgaacc gcagcgtacc
     2161 gctgggcccg ccggagcgcc tgatcataat tgccttgttg ttttttgtct gcttccgaaa
     2221 gcgttttcag tgtttccagg agcatgggct ggatgacata actgttatag taatcccagt
     2281 gaaaatgggg accgtctcca tatgtcccgt cgcccttata ccataattga tgctggcgga
     2341 tagcgtagtc catgcgcatg cggtcgccat cctggccaaa cttcagcagg gcggcctcaa
     2401 tgaccgcact gaataataac cagttggaat agccaggcgt aataactctc gatgatttca
     2461 gcgcggcaat aatatgggcc tttgtttgcc catccagcgg ctcccaaagc tgatgcggtg
     2521 cgcgcagcag ggcctgggca agaaatgccg cgtccaccag gggctgactg ccttcattga
     2581 aattcataaa atccggtgcg tggggatccg tggcgttatg caggcctgcc cgcgccagtt
     2641 caatatattt ttttctgagc tgaccctccg gagtcgggtc agcaccgagt tccagccagg
     2701 gcgccattcc ggccagcaaa cgaccgaatg cttccaggta agtataattt ctccggtccg
     2761 tttcttttgc ctcgacgggc attttctgtt tcagttcatt tttgtccagc gcacccagca
     2821 ctgggtcagc tatccgggtc agggacctta ttaaatagtt ccgttcagcc aggccggatt
     2881 catcttttga ggactgggca ggcaaaggct gtggaaatgt ctgcaagagc aaaataaaaa
     2941 ggatcgatgc aattttcatg gtgagagtat atgtcggtgc aaattatata aaaggatata
     3001 cggaaatgat ggtaaatagg tcaaatggta tactttgcga ataataaaag cgctgtattg
     3061 tcccgagcag ttacccggac gggtaaaata acagaaaccc ggaacaaaat tattccatgg
     3121 aatatcatgg ggaacaggac ccggcaagcc gtacggataa ttcactgaac agacgggtgg
     3181 agatatccct gcggcttccg gaacgccagg ttgcccgggc cgacagcgac caattatcca
     3241 tacgaaaaac atatacgctg agtaaattat atttcaggcc ggacgaagct attctcgaac
     3301 cttcatcgat gccctacctg gataacgtgg ccaatatatt aaaaacctat accagcggcc
     3361 tgttcgaaat caggggcctt gtaaactggg aggctccgcc agtcgcatcc gccgattcgg
     3421 gctataagaa aaagatggac gaattgtcca ccgcgcgcgc caggctggtt tacgagatcc
     3481 tgattgacaa gggcattccg ccggaaagaa tgaggtataa aggaatgggc aactcgcaaa
     3541 tgctctaccc ctatgccagt acggatgaag aaaagcggaa gaacatgcgg gtggagatcc
     3601 tgatactaac aaaacagaag caaacccatt aaataccatg aaaaaaatct caggcctaac
     3661 catccttgtt tttttatccg gatgcgccgg caaaagcgat ccttttcccg aattatcttc
     3721 ctttcacaag ccggtcaact atcctatttt aaaaggcgat ccgtcaataa cctttttcga
     3781 ttctttaaag atggagaacg tagcctggaa gcgcgccgat gtctttaacc cggcagcgat
     3841 cgtaaaagat aataagatct atttgctgtc ccgctgcgaa gacaatccgg cagccatcct
     3901 ggggggccgt acctcacgca taggcctgtc cgttagtgac gacggaatac acttcagctc
     3961 tctcggctct cccgtgctct atccggcaaa cgacgccttc aaagaatacg attacccggg
     4021 cggttgcgaa gatccaagag cggtggaaac agaagacggg ttatatgtca tggcctatac
     4081 ttcctggaac tataaagttc cacgcttatc catagccgtt tcaaaagacc tgcttcactg
     4141 gtcaaaaaaa ggccccgctt ttgccaaagc ataccagggc cggttcctgg atctggcaac
     4201 caagtcggga tcgatgctga caataatgaa aaataaccgg cccgtactgg cgaaacgcaa
     4261 cgggaaatat tggatgtatt ggggagagca ttttgtcaat ctcgcctggt ccgttaattt
     4321 atccgactgg tatccgttgc tggattcaaa taataacctc aaaaaaacaa tcgtcacccg
     4381 gccgggtaaa tttgacagcg atctggatga atgcggacca cccgctctaa taaccggaaa
     4441 aggaatcctg ttaatctata atgggaaaaa ttcggccggc cgaaaggcgg atccttcctt
     4501 accggaagga acttattcaa ccggaagcgt cttattcagt ttgaaagata tggagaccgt
     4561 catcgaccgg ccggaccatc ccttcctgaa accatccctg ccgcatgaac tcaccggcca
     4621 gtatacggca ggaaccactt ttgcagaagg gcttgtctat tttaaagaga aatggttttt
     4681 gtattatggc acggcagact cgtttgtagg tgttgccgta accggaaata aggactgagg
     4741 cgagccggaa atcgaaaccg atttgccggt aatttagcgg aactttgctc ccggcaatca
     4801 tccggccaaa acaaataata tggaagatgt caaattgaaa ttaaatgaaa aaggccgcgg
     4861 ctcattttat atcgcggtcg gagacgaaca gcttgccatg atggagatcg gtatttcaga
     4921 aaataccctg acggcttatc ataccgaagt ctccccgaaa gccgggggga aaggcctggg
     4981 aaaagaactg ctgatcgcca tggccgccta tgcccgtgaa cataaatata aagtgattcc
     5041 gctttgtcct tttgtgaacg cccagtttaa acgccacccg gaagaatact cggatatatg
     5101 gctcaaaccg gttccttaag cccggcagac caccttcttt tcattctaat gagtgctgat
     5161 catctgcaga taaagaatat agtttccgcc gggaaaaata ttcaggaggc cgggcgggtt
     5221 ttgatcatga tgcacggccg gggcgctacg gcggaaagca ttctttcgct ggccggtcag
     5281 ctccgcgtga acgattttgc actgatcgct ccccaggcat taaacaggac ctggtatcct
     5341 tattccttta tggccactcc cgcccaaaat gaaccggccc tgtccgcttc tctatccagg
     5401 ttaaaagaac tgctgaacca gctgaacggg cagggcattt cttcaaaaaa tatttatttc
     5461 ctggggttct cccagggcgc cagtttaatc ctcgaatttg catgccggaa cgcgacccgg
     5521 tacggcggta tcgttgcatt tacgggaggt caggccgggg accagatcta ttcagaaaac
     5581 tataccggca attttgacgg cactccggta ttcctcggta ccagcgatcc cgaccagcac
     5641 gtcccggtga taagagtaaa tgaaacggca accatcttac agaaaatgaa cgccgccgta
     5701 acaaaaaagc tctaccctta cctgggacat accatcaact ccgatgagat ggattcggcc
     5761 aaccggctga tatttgaccc cgaaacttaa cccccggccg ttcactccta tttatcccta
     5821 catttatccg gcaataaccc tttttttaaa acatcccggc tgctgtgcaa taagccccga
     5881 aacacagctc aatggcctga aattgaaaaa tgtatgaact ggttgctgtt ctccgaaatc
     5941 ctgtatacgc tggtgctgat gatggtttgt ctgcgtatta taacagacac ccggaccacc
     6001 aataaggcac tatcctatct cctttttgcc gtttttgttc cattcttcgg aatgatcttc
     6061 tatttccttt ttggcatcaa ttaccggaaa agaaagattt acagcaaaaa gctttatgaa
     6121 aatgatgcct tggccaacaa aatgagagga gatatttatc aatattcaaa acagaccttt
     6181 aacgaatgcg atgcttccgt tcaaagcagc agggagttag cctatatgtt attaaaggac
     6241 acaacaagtc cgttgacggg aaacaattcg gtaaagctgc tgatgaatgg agaaaacaag
     6301 ttccccgaag tgatccgggc cctgaaagag gcaaaacatc atattcacgt tgaatattat
     6361 atttatgaag acgatgagat tggccgcaca atcgaaacca tattgattca aaaggccgct
     6421 gaaggcgtgg aggtacgctt catttatgat gattttggaa gccgttccat acgaagaaaa
     6481 ctggttcccc ggctgaaagc aaacggagta cacgcgtact cctttttccg gatatatttt
     6541 atcccctttg ccaaccggct caactaccga aaccaccgga aaataattgt cgtggatggc
     6601 cgcaccgctt tcgtaggcgg catcaacgtg agtgaccgct atatcaattc accggaggat
     6661 acaaaaaaat tattctggcg ggatacccat ttacgcattg acgggccggg agtgcagtac
     6721 ctgcaatacc tttttttgtg cgactggaac ttttgcacgg agaagaaact gcaaccggac
     6781 agccactttt ttcccccgct gaattcgtac ccggtaaaag acgacaaggt ggtgcaaatc
     6841 gcagccggcg gtccggactc tgaaatgccg accattttgt tttcccttct gaaagcggtg
     6901 aacctcgcaa cgaaggaaat tcttatttcc actccttact ttattcccgg agatggtttg
     6961 attgaagccc tgatgatcgc gtccctggga ggcatcgctg tgaaactcct ggtgccggga
     7021 caatcggatt ccctgcttgt caattcggct gcaaaatcct attacaagga tctcctgcaa
     7081 acgggtgttg aaatctacct gtacaaaaga ggattcctgc acgccaaaac cctggttgca
     7141 gaccggaaaa tcgccattgt agggaccgcc aacatggact tccggagctt tgaactcaat
     7201 tttgaggtta acgcgatcgt gtacgatatg gaaatcgcaa atcagctcag cgacgttttt
     7261 tatgcggatc tgaaagacgc tgaaaagatt gatgccgtcg cctggggaaa gcgggcaaaa
     7321 tacaaacgcc tgctggagaa aacggccagg ctggtatctc ctcttttata aattcttccg
     7381 gttgcgttag gctgaatacc ggatttaaag caggccattc accgcatctg ccagcgatcg
     7441 gtagctggcg tcatagctgt tcagccggtt tccgttcagg aaaaagctgg gcgttccgtt
     7501 cacaccactg cgcactccgc cttcaaaatc agtttccact tttaaaagca ggtccccgga
     7561 ccggag
//
LOCUS       DASYCU010000296         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3399697, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000296 DASYCU010000000
VERSION     DASYCU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3399697"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VGM24_06040"
     CDS             complement(<1..1930)
                     /locus_tag="VGM24_06040"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY4154963.1"
                     /translation="MPELGQESAQKPPIPVALTYMNSDFWQLSVDLLNKPHTPIRYHY
                     LLQLPDMTRISEWGNDREIPVSAKSASEIQTIDTWNYAGEYENAFYTSAFQQILLPAH
                     EKKSGAKTPKNFTHIFKVKAPLLEANQCLCLAGSGEGMKEWSTEDPIRMKKEGPWWTA
                     SVNLPREFLPVSYKYAVWDRKHRQFLYFENGPNRSLPGDALDQKLIIVHDGFVRLPNN
                     TWKGAGVSIPVFSLRSEHSFGTGEFTDLKLLADWAKKTGLKLIQILPVNDTSATGTWL
                     DSYPYAAISAFALHPLYINLEETAGKKYADRLKPLRKKQKQLNELPDLDYETVISSKM
                     AVLRELYELQKKDLNEDPEYLKFFENNKFWLTPYAAFSYLREINGTPDFTKWKTYGRF
                     NKLSIDRLTAPQSRQYDQVALYYFIQYHLHLQLSRAADYAHDKGIVIKGDIPIGVYRY
                     SCDAWMAPELYHLDQQAGAPPDSFALKGQNWGFPTYNWEQMEKDDYRWWKNRFEQMAI
                     YFDAFRIDHILGFFRIWSIPVQETEGIMGRFVPAVPVYRLEFDERNIWFDHDRYTKPF
                     INDAVLWELFGKDSEKAKPVYFEEIPGGNYRIKDFVDDQRKVQDYFAGKTDDAYDMLS
                     REALLSLISNVIFFEVPES"
     gene            2140..2295
                     /locus_tag="VGM24_06045"
     CDS             2140..2295
                     /locus_tag="VGM24_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154964.1"
                     /translation="MEKTKKYTGLFWALFIISVIAFFGVYITIGGPCIMILPFVCTFL
                     AKALDIM"
     gene            complement(2417..2614)
                     /locus_tag="VGM24_06050"
     CDS             complement(2417..2614)
                     /locus_tag="VGM24_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154965.1"
                     /translation="MKQDVMPSIVRMDQETLKKLTTEVKETLATGYDSPKAKSRFHSV
                     NLWNIHRNWKLASRISGKRGI"
     gene            complement(2881..>4696)
                     /gene="dnaK"
                     /locus_tag="VGM24_06055"
     CDS             complement(2881..>4696)
                     /gene="dnaK"
                     /locus_tag="VGM24_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY4154966.1"
                     /translation="RTTPSVVAFLKNGERKVGDPAKRQAITNPVNTITSVKRFMGRRY
                     DEVTNEISHWSYKVVKGDNNTCRIDIDGRQYTPQEISAMILQKMKKTAEDYLGQEVTE
                     AVITVPAYFNDAQRQATKEAGEIAGLNVRRIVNEPTAAALAYGLDKAGKDEKIAVFDL
                     GGGTFDISILELGDGVFEVKSTNGDTHLGGDDFDKILIDWLAGEFKKDENIDLRKDPM
                     ALQRLKEASEKAKIELSSSTETEINLPYVTAVDGVPKHLVKKLSRAKFEQLSDSLFER
                     CLKPCEQALKDAGLPASKIDEVILVGGSTRIPKVQEIVEKFFGRKPNKGVNPDEVVAI
                     GAAIQGAVLTGEVKDVLLLDVTPLSLGIETMGGVMTRLIESNTTIPTKKSETFSTASD
                     SQPGVQIHVLQGERPMANQNKSLGMFNLDGIPPAPRGVPQIEVIFDIDANGILHVTAK
                     DKGTGKEQKIRIEAGSGLSKEEVEKMRAEAKANETTDKAAREKVDKLNQADSLIFQTE
                     KQLKEFGDKISADKRSPIESALAKLKEAHKSQDSAQIDAAVAEINNAWTAASEEMYKA
                     TQGQATASGNPGGTNAEGQPNADKNAENVTDAEFEEVK"
BASE COUNT         1036 a         1172 c         1121 g         1367 t
ORIGIN      
        1 cagattccgg cacctcaaag aaaattacgt tggagatcag gctgagcagt gcttcgcggc
       61 taagcatatc ataggcatcg tcggttttgc cggcaaaata gtcttgtact ttccgctggt
      121 catccacaaa atccttgatc cggtaattgc cgccgggaat ttcttcaaaa taaacgggtt
      181 tggctttttc ggagtccttt ccgaacagtt cccagagcac cgcatcgttg atgaatggtt
      241 tggtataacg gtcatgatca aaccagatat ttctttcgtc aaattccagg cggtataccg
      301 gcacagcggg tacaaaccgg cccatgattc cttccgtttc ctgaaccgga atgctccaga
      361 tgcggaaaaa acccaggata tgatcgatcc ggaacgcgtc gaaatagata gccatttgtt
      421 cgaagcggtt cttccaccag cggtaatcat ccttttccat ctgttcccag ttataggtcg
      481 gaaaccccca gttctggcct tttaatgcga atgaatccgg cggtgcgccg gcctgttgat
      541 ccaggtggta aagttcaggc gccatccacg catcgcaact gtaccggtaa acgcctatcg
      601 gaatatcgcc tttgatcacg atccctttat catgtgcata atccgcggcc cggctaagct
      661 gcaaatgaag atggtattga ataaaatagt aaagggccac ctgatcgtat tgtctggact
      721 gcggggcggt cagccggtcg atgctgagct tattgaagcg cccgtaggtt ttccatttgg
      781 taaaatcggg cgttccgttt atctccctta aataactgaa agcggcatag ggagtcagcc
      841 agaatttatt gttttcaaaa aacttcaggt attccgggtc ttcattgagg tcctttttct
      901 gcaattcgta gagttccctg agcaccgcca ttttactgct gatcaccgtt tcgtaatcca
      961 ggtccggcag ttcgttaagt tgcttttgtt tttttcgtaa cggcttaagg cggtctgcgt
     1021 attttttccc ggctgtttct tccaggttga tatagagcgg gtgtagcgca aaagcggaaa
     1081 tcgccgcata gggataggaa tcaagccagg ttccggtggc agacgtatca ttcaccggaa
     1141 ggatctggat cagtttcaat ccggtctttt ttgcccagtc tgccagtaat ttgaggtcgg
     1201 tgaattctcc tgttccgaat gaatgttcac tccggagact gaataccggg atactcaccc
     1261 ccgccccttt ccaggtattg ttcggcagcc ttacgaaacc atcgtgcacg atgatgagtt
     1321 tctgatccag cgcatcgccg ggcagggatc tgttcggacc gttttcgaaa taaaggaatt
     1381 gccggtgctt ccggtcccag accgcatatt tatagcttac gggaagaaat tcacggggaa
     1441 gattgacgga agcggtccac caggggcctt cctttttcat ccggatcggg tcttccgtgg
     1501 accattcttt cattccttcc ccgcttccgg ccaggcaaag gcactggtta gcctccagca
     1561 agggcgcctt taccttaaag atatgggtga aattttttgg cgttttggcg ccggattttt
     1621 tttcatgggc cgggagcagg atttgctgaa aagcggaagt ataaaatgca ttttcatatt
     1681 ccccggcata attccaggta tcgatcgtct ggatttcgct cgctgatttc gcagacacgg
     1741 gtatttcgcg gtcgttcccc cattcgctga tccgggtcat atcgggcaat tgcagcaggt
     1801 agtggtaccg gatgggggta tggggcttgt tcagcaggtc cacgctcaat tgccagaaat
     1861 cggaattcat ataggtaagg gctaccggta tgggaggctt ttgcgcactc tcctgcccca
     1921 attcaggcaa attcccgctt acaaaaagtg tctgtccggg tttggtgtta aatcttaagt
     1981 agaaatgtag tttcattctc cagtcaaatt cagaaaatcc attcacgccc tttctgtagc
     2041 ggatgagtcg cgaaattacc aaaagggaaa cttgaaataa aattaaagtg attcgggtac
     2101 gggattttga aatatatttg cgaatctaat ttctcccgca tggaaaaaac taaaaaatac
     2161 acgggtctgt tctgggctct tttcatcatt tcagtgatcg catttttcgg ggtctatata
     2221 accatcgggg gcccctgcat catgatcctt ccgtttgtat gcacttttct tgccaaagcc
     2281 ctggatatca tgtagggttt ccctcctcca accttccgga ataaaaaaca ccgcctgaag
     2341 cagacggtgt tgtcagatgg ttaacccata tattttcctt tccacgcgga aggcatttga
     2401 ttattgaaga tatggatcag atcccccgtt ttccggaaat ccgggaagcc agtttccagt
     2461 tgcggtgaat attccataga ttaacggaat gaaaacggct ttttgctttc ggtgaatcat
     2521 aaccggtagc gagggtctct ttaacttcag tggttaattt tttcagggtt tcctggtcca
     2581 tccggacaat cgagggcatg acatcttgtt tcataaatga tcgtgtttaa aatttaagat
     2641 tgatgagcaa ttgaaggaag aacacgaaag aatcgttaga aagaagtgtc ggatccagaa
     2701 ctggcttgcc aggttaaact attgctcaat tgcgatgcaa atataccact tattattgat
     2761 tcctgcaagc ttttaatcca atgttttggg aataagtgtc attttgtact ttattaacag
     2821 atacaataaa acaggaaagg ccgtcgttac agacggcctt ttccaaatcc atatatccgg
     2881 ttattttact tcttcaaatt cagcgtcagt cacgttttcc gcgtttttat ctgcattcgg
     2941 ctgcccttct gcgttagtgc cgcccgggtt tccggaagcc gttgcctgac cctgggtcgc
     3001 tttgtacatt tcttccgatg cggcggtcca ggcattattg atttcagcca cggcggcatc
     3061 gatctgggcg ctgtcctgcg acttgtgcgc ttctttcagt ttcgcgaggg cggattcgat
     3121 cggtgaccgt ttgtcggcgg agatcttgtc cccgaattcc ttcagctgtt tttctgtctg
     3181 aaagatgaga ctgtctgcct gattcagctt atccactttt tcgcgggcgg ccttatcggt
     3241 cgtttcattg gccttggctt ccgctctcat tttttccacc tcttccttgc tgagtccgct
     3301 tccggcttca atccggatct tctgttcctt tccggttccc ttatccttag ccgtaacgtg
     3361 caggatcccg tttgcgtcga tatcaaaaat gacttcgatc tgcggaacgc cccggggcgc
     3421 cgggggaatg ccgtcgaggt taaacatgcc cagactctta ttctgattag ccatgggcct
     3481 ttctccctgc agaacatgga tctgtacgcc cggctggctg tcgctggcgg tggagaaggt
     3541 ctctgatttc ttggtcggaa tggtggtatt gctttctata agccgggtca tcaccccgcc
     3601 catggtctcg atgccgaggc ttaagggggt aacatccagc aaaagcacgt cttttacttc
     3661 ccccgtcagg acggccccct ggatggcggc accgatagct accacttcat ccgggttcac
     3721 tcccttattg ggttttcttc caaagaactt ttcaacaatt tcctgcacct tcggaatccg
     3781 ggtactaccg cccaccagga tcacttcatc aattttggaa gccggcaaac cggcatcttt
     3841 caaagcctgt tcacagggtt tgagacatct ttcaaaaaga ctgtccgaca attgttcaaa
     3901 tttcgcccgg ctcagctttt tcaccagatg tttgggaacg ccatccacag ccgttacgta
     3961 gggcaggttg atctctgtct cggtagagga agacaattcg atttttgctt tttctgaggc
     4021 ttctttcagt ctttgaaggg ccatcggatc tttgcggaga tctatgtttt catctttttt
     4081 gaattccccg gcaagccagt cgatgaggat cttatcgaaa tcatccccgc ccaggtgcgt
     4141 atctccattg gttgatttta cttcaaaaac cccgtctccc agttccagga tggagatgtc
     4201 aaatgttccg ccacccaggt cgaaaacggc aattttttca tcttttcctg ccttgtccag
     4261 cccataagcc agggcagccg cggtaggttc gtttacgatc cggcgaacgt tcagacccgc
     4321 aatttcgccg gcttctttgg tagcctggcg ctgggcatca ttaaaatagg ccggtacggt
     4381 aatcacggcc tcggtcactt cctggcccag gtagtcttcc gcggttttct tcattttctg
     4441 aagaatcatc gcactaatct cctgaggtgt atactgacgg ccgtcaatgt caatacggca
     4501 agtattattg tcacccttca ccactttata gctccagtgg ctgatttcat tggtcacttc
     4561 gtcgtaccgg cggcccataa aacgcttcac cgaagtgatc gtatttaccg ggttggtgat
     4621 ggcctgccgt ttggccggat ctccaacttt acgttcgcca tttttcagga aagcaaccac
     4681 cgacggtgtg gtcctg
//
LOCUS       DASYCU010000297         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4270088, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000297 DASYCU010000000
VERSION     DASYCU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4270088"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VGM24_06060"
     CDS             <1..260
                     /locus_tag="VGM24_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HEY4154967.1"
                     /translation="LLFTFGKLLLKWLLSYSNMQTIYGASTSSVLLLLFVFYSSIIFY
                     FGACFTKVWAIHQKHPIQPGKNAVLYQWSQVKGNKVSTVGK"
     gene            304..2055
                     /locus_tag="VGM24_06065"
     CDS             304..2055
                     /locus_tag="VGM24_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4154968.1"
                     /translation="MKKISPFLLTLLISGLGFCQSPAPKYPVDPASVEHPGVPKGEVL
                     KFRFDRSAIFPGTVRDYWVYIPAQYDPSKPACVYVNQDGIQWNAPAVFDNLINSREMP
                     VTIGVFVRPGIIPAANNDHALDRVNRSFEYDGLGDAYARFILKELLPEVEKQKASDGR
                     PLRLSEKGNDRAIGGSSSGAVCAFTAAWEYPLEFSRVFSAIGTYTGLRGADRYPILIR
                     KYEPKPIRVFLQDGSGDLNIYGGDWWKSNESMERALDFAGYEVRHVWGEGGHNGDQGA
                     SVFPDAMRWLWKDWPKPVLTGNTKNQFITDILSDKDWELVAQGYSFTEGTAVNAAGEV
                     FYQDIPNAKTYKVGLDGVLSALPLDSKKAAGTIYGPDGKRYVAAGETKQILSYDPNNK
                     VSVIADSINGNDLVVANNGNIYVTVPDGTEKPSKLYLIRPNGEKMIVDQGLKFANGIA
                     LTPDQTQLYVSESATHWIWIYSINPDGTLANKQRYGWLHVPDTEENAWPDGIRCDKDG
                     RLYAATRLGIQVLDQLGRVNAILPVPENKVYNLCFGGPGFDILYIAAGDKVFRRKVKV
                     HGANGFEPPLKPASPKL"
     gene            complement(2060..4726)
                     /locus_tag="VGM24_06070"
     CDS             complement(2060..4726)
                     /locus_tag="VGM24_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY4154969.1"
                     /translation="MGNRALFLAVFFVSSSAFAQPALPYKNPALPVEERVNDLLSRMT
                     PTEKFWQLFMIPGDLGRGDSLQYKNGIFGFQVSTAAKGDAGGQMLNYRASENALSLAK
                     KINAIQEYFVNNTRLGIPVIAFDEALHGLVRGGATCFPQAIALAATFDTALMHEVAGA
                     IATETKARGIRQILSPVINIASDPRWGREEETYGEDPYLTSRMAVAFVSAFEKMGIVS
                     TPKHFIANVGDGGRDSYPIHFNERLLDEIYFPPFKACIQEGGSRSVMTAYNSLDGISC
                     SSNSWLLQKKLKGDWNFKGFVISDANAVGGDVVLHYTAKNSAEAGQHAINNGLDVIFQ
                     TDYKHYKLFISPFLNGAIDSNRINDAVARVLRAKFELGLFENPYTPEAEAAKWVDNAG
                     HKYLAGRAAVESIVLLKNARNTLPFHKDIRRIAVIGEDAVEARLGGYSGPGNHKVSIL
                     DGIKQRSGDSIQVLYEPGCGLRTKEWEPVPSGYLSSSLGGKPEQGLKGEYFNNITLEG
                     TPVLTRMDKTLNFSWTLSSPDRNISHDFYSVRWTGKLKAPQSGMVKIGLDGNDGYRLY
                     LDNVLVIDRWRKQTYSTRLVPFHFEKDKSYDIRVEFYEPDGNAHIKLIWNANISDDWN
                     AKINRAVAAAQKADVAVIVAGIIEGEFQDRAMLGLPGHQEELIRQVAATHKPVVVLLV
                     GGSAITMGDWLDEVNSVMDVWYPGEEGGHSVAQVLFGDYNPAGRLPVTFPISESQLPL
                     VYNHKPTGRGDDYNNLSGLPLFPFGFGLSYTHFGYRDMQIEKNNISTSDSTIVKYKVK
                     NEGAVEGDEVVQLYIRDMLSSVSRPVMELKGFQRIHLLAGEEKQGSFTILPAMLSMLD
                     KNLRTVVEPGDFRIMIGSSSRDIRLKETLTVR"
BASE COUNT         1186 a         1262 c         1209 g         1224 t
ORIGIN      
        1 gtctgctttt caccttcgga aaattgctgc tgaagtggtt attgtcatac agtaatatgc
       61 agaccattta cggcgcttcc acgtcttcgg tcctcttact tttatttgtc ttttattcct
      121 ccatcatttt ttatttcgga gcctgtttca ccaaggtctg ggcaatccat cagaagcatc
      181 cgatccagcc gggaaaaaat gccgtcctgt atcagtggtc gcaggttaag ggaaataaag
      241 tttcgacggt tgggaaataa gggaattgtc cggaagtgta tatttacagg acaagcttcc
      301 agcatgaaaa aaattagccc ctttttattg accctgctga tttccggcct gggtttttgc
      361 cagtcgcccg ctcccaaata cccggtggat ccggcctcgg tagaacatcc gggcgttcca
      421 aaaggggaag tattaaaatt ccgttttgac cggtctgcca ttttcccggg aaccgttcgg
      481 gattactggg tctatattcc agcccagtac gatccatcca aacccgcctg cgtctatgtg
      541 aaccaggacg ggatccagtg gaacgcaccc gctgtatttg acaatttgat caacagccgg
      601 gagatgccgg tcaccatcgg ggtcttcgtg aggcccggaa taattcccgc tgcaaataat
      661 gatcatgcgc tggaccgcgt gaacagaagt tttgaatacg acggactggg agatgcgtac
      721 gcgcgtttta ttctgaaaga attattgccg gaagttgaaa aacagaaagc gagtgacgga
      781 aggccgctgc ggttatctga aaaggggaat gaccgggcga tcggcggatc cagcagcggc
      841 gcggtttgcg cgttcaccgc cgcctgggaa tacccgcttg aattttcaag ggttttcagc
      901 gccatcggta cctataccgg cctgcggggc gccgaccgtt atccaatcct gattcgcaaa
      961 tatgagccca aacccatccg cgtgtttctg caggacggat ccggcgacct gaacatctat
     1021 ggaggggact ggtggaaatc aaatgaatcc atggagcgcg ccctggactt cgcaggatat
     1081 gaggtaaggc atgtctgggg ggaaggcggg cacaacggcg accagggagc ttccgttttt
     1141 ccggatgcga tgcgctggct gtggaaggac tggccaaaac ccgtgctcac cggaaataca
     1201 aaaaatcagt tcataaccga tattctttca gataaagatt gggaactggt ggcacaggga
     1261 tattcattta cggaaggaac ggcagtgaac gccgcgggtg aagtttttta tcaggatatt
     1321 cccaatgcaa agacctataa ggtcgggctt gacggagtcc tttccgcact gcccctggat
     1381 tccaaaaaag cggccggcac catttatggc ccggacggta aacggtatgt ggcggcgggc
     1441 gaaaccaaac agatcctttc ctatgacccg aataataaag tgagcgttat cgcagacagc
     1501 attaacggga atgatctcgt ggtagcaaac aacggcaata tttatgtgac cgttccggat
     1561 ggaacggaaa aacccagcaa actatacctg attcgtccca atggagaaaa gatgatcgtg
     1621 gatcagggtt taaaatttgc aaacgggatc gcgctgacac cggatcagac ccagttgtat
     1681 gtttccgaat ccgccacgca ctggatctgg atttacagca tcaacccgga cgggacactg
     1741 gccaacaaac aacgctacgg atggctgcat gttccggaca cggaagaaaa tgcgtggccc
     1801 gacggaatcc gttgcgataa agacggccgt ttgtatgcgg caacccgtct gggcatccag
     1861 gtgctcgacc agttggggcg ggtgaatgct attttgcccg tgccggaaaa taaagtatac
     1921 aatctttgtt tcggaggacc cggttttgac attctttata ttgctgcggg cgacaaggtc
     1981 ttccgtcgga aagtgaaagt gcatggcgcc aacggctttg agccgccgct aaagccggca
     2041 tcgccaaaac tctaattcgt cagcgcaccg ttaaggtttc tttcaaccgg atatcccggg
     2101 aagatgatcc gatcatgatc ctgaaatcgc cgggttccac tacggtgcgg aggtttttat
     2161 ccagcatgct taacatcgcc ggcaggatgg taaatgatcc ctgtttttct tctcccgcca
     2221 atagatgaat acgctgaaac ccctttaatt ccattaccgg ccgggataca ctggaaagca
     2281 tatcccggat atacagctga accacttcgt cgccttcaac ggctccttca ttttttactt
     2341 tgtatttaac gatcgttgaa tcgcttgttg aaatattgtt tttttcaatc tgcatatccc
     2401 ggtatccgaa atgggtatag cttaatccaa atccgaaagg gaaaagcggc aaaccgctga
     2461 gattattata atcatcgcct cttccggtgg gcttgtggtt ataaaccagg ggcagctggg
     2521 attcggaaat ggggaaagtg accggcaggc ggccggcagg attgtagtcg ccgaataata
     2581 cctgcgcaac ggaatggccg ccttcttctc ccggatacca aacatccatt acgctgttca
     2641 cttcgtccag ccagtcgccc atggtaatag cacttccacc caccagcagt acgaccacgg
     2701 gtttatgcgt ggcagccacc tggcggataa gttcttcctg gtgtccgggc aagccgagca
     2761 tggcccggtc ctggaattcc ccttcaatga ttcccgccac gatcacggcc acatctgctt
     2821 tttgcgcagc cgctacggcc cggttgatct ttgcattcca gtcgtctgaa atattggcat
     2881 tccagatcag ttttatatgg gcattgccgt ctggttcata aaattcaacc cggatatcat
     2941 agcttttgtc tttttcaaaa tgaaaaggga caagccgggt actgtaagtc tgtttccgcc
     3001 agcggtcgat cactaaaaca ttatccaggt ataaccggta gccatcattt ccatccagtc
     3061 cgatcttaac cataccggac tgcggcgcct tgagtttgcc cgtccacctc accgaataaa
     3121 aatcgtgact gatattccgg tctggcgaag aaagggtcca gctgaaattg agggtcttat
     3181 ccatccgggt cagcaccggg gttccttcca atgttatatt attaaaatat tctcctttta
     3241 agccctgctc cggcttcccg cccaggctgc tgcttaaata accggaaggg accggttccc
     3301 attcttttgt tctcaaaccg caacccggct cataaaggac ctgtatggaa tcgccggatc
     3361 tttgctttat cccatccagg atgctcactt tatgatttcc cggcccgcta tagccgccga
     3421 gtctggcctc aacggcatct tcgccaatta ccgcaatccg gcgaatgtct ttatgaaagg
     3481 gaagggtatt tcttgcattc tttaataaaa cgatggactc caccgcggct cgtcctgcga
     3541 ggtatttatg accggcattg tccacccatt ttgccgcttc cgcttccggt gtatacggat
     3601 tttcaaacaa ccccagttcg aacttggccc ttaagaccct ggccaccgcg tcgtttatcc
     3661 ggttactgtc aatcgccccg ttgagaaaag gggaaatgaa taatttatag tgtttataat
     3721 cggtctgaaa aataacatcc agcccgttat tgatggcgtg ttgcccggct tccgcagaat
     3781 tttttgcggt gtaatgcaat accacgtctc cgcctacggc gttggcgtcc gatataacaa
     3841 aacccttaaa attccagtcg cccttgagtt ttttttgcag caaccagctg ttggatgaac
     3901 aggatatgcc gtcaagcgaa ttataggcag tcattaccga gcgcgaaccg ccttcctgaa
     3961 tgcaggcttt aaaaggcggg aaataaatct cgtccagcag tctttcatta aaatgaatgg
     4021 gataactgtc ccttccccca tcccccacat tggctataaa atgtttcggg gtgctgacaa
     4081 ttcccatttt ttcgaaggcg ctcacaaagg caaccgccat ccgggaagtc aggtaagggt
     4141 cttcgccata ggtctcctcc tcccgccccc agcgcggatc actggcaata ttgatcaccg
     4201 ggctcaggat ctgccggatg cctcttgcct tcgtttccgt ggcaatggcg ccggccactt
     4261 catgcatcaa cgccgtatcg aatgtggcgg ccagggcgat ggcctgggga aagcaggtgg
     4321 ctccgccgcg caccaatccg tgcagagctt cgtcgaaggc aatgaccggg atccccaacc
     4381 gggtattgtt cacaaaatat tcctgaatgg catttatctt ttttgccagg ctgagcgcat
     4441 tttcggaagc gcggtagttc aacatctgtc ccccggcatc cccttttgcc gccgtactta
     4501 cctgaaaacc gaaaattccg tttttatact ggagcgaatc cccgcgaccc aggtcgccgg
     4561 ggatcatgaa aagctgccag aatttttccg ttggcgtcat cctcgacaac aggtcgttca
     4621 ccctttcctc taccggcaag gccgggtttt tatagggaag ggcgggttgc gcaaaggcgg
     4681 aggaagaaac aaaaaaaacg gccagaaata atgccctgtt tcccataaat aatgattggt
     4741 aaacttccgc gtggcgctga agaatatttt tcaaaataaa actatatttt agaaatagcc
     4801 cgtgttttta atacaatggc gttcatttgc cttatattga attcccaaaa cggactgcat
     4861 gaaacgattc ctggtcgcgg g
//
LOCUS       DASYCU010000298         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1079883, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000298 DASYCU010000000
VERSION     DASYCU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079883"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGM24_06075"
     CDS             complement(<1..513)
                     /locus_tag="VGM24_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154970.1"
                     /translation="MKMEFKEVYITKTATYFPNEPVSNDEMEEFLGLINKSPSKSKRI
                     VLRNNGIKRRFYALTREGKPTHTNAEMTAEAIRRMLDNDPEQIREIDLLVCGTSSPDQ
                     IMPSHAVMVHGELPESRSIEVVSPSGVCCAGMHAFKYAYMAVKTGGAHHAVATGSERF
                     SAALKSEIFED"
     gene            complement(521..1420)
                     /locus_tag="VGM24_06080"
     CDS             complement(521..1420)
                     /locus_tag="VGM24_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154971.1"
                     /translation="MGKKVLAVFYTQSGQLAEIIDHFTQPLLDAGASVEKLRIYPGVP
                     YPFPWTGESFFSVMPDSVNQVPGPLQPISFKEEFYDLIILGYQAWFLSPSIPANSLLN
                     DPKFRALARNTPVITITGARNMWISAIEKIKPTLRNIGAKLVGNIALVDRHHNFVSFV
                     TLFHWMFKGRKDRYLNIFPKPGVSDADIVHTKVFGEIVLPHLKNSNWQGLQEELVDAG
                     GLVVKYPLMFIESKARKIFKVWAGIISKRKKRTAWLVAFKYYLLIALFIAAPIILTVD
                     AIFFKPFLSGRIKRQKQYYSEVN"
     gene            complement(1434..1925)
                     /locus_tag="VGM24_06085"
     CDS             complement(1434..1925)
                     /locus_tag="VGM24_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154972.1"
                     /translation="MRKIKWVLGMLALVAGSSPGFAQSAGNIDRTAFYQAIRQDKKDL
                     VNEQLKILKMAPAELRQAFEGALTMKKAGLGGSPPQKLKLFRQGRHELEAAIKRDQNN
                     VEYRFLRLIIQENSPAVLGYRDDLPGDRRIIEKSYKTLPGELQRVIEDYSKKSKILKL
                     QVS"
     gene            complement(1950..3254)
                     /locus_tag="VGM24_06090"
     CDS             complement(1950..3254)
                     /locus_tag="VGM24_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4154973.1"
                     /translation="MIPAQTIFMKPEEINRTQEKKLLELLQHLKGHSPYYQRIFKEKK
                     IDLNRIRGLGDLGLLPTTVKEDLQKYNDEFLCVPRTKVIDYTSTSGTLGAPVTVALTE
                     KDLQRLARNEYGSFHCADGKPEDIYQLMLTLDRQFMAGMAYYSGIRKLGAGIIRVGPG
                     VPSLQWETIIRLRPSAIVAVPSFILKLIQYASDYHIDIRSSAVRKAICIGENIRQNDF
                     SLNVLGRKITEKWNIQLYSTYASTEMQTAFTECSAGRGGHQQPELVITELLNEQDQPV
                     PDGEPGEVTITTLDVEGMPLLRYKTGDICIAHREPCSCGRFSMRLSPVIGRKKQMIKF
                     KGTTLFAPALYDLLNGMDDITDFVAEVYSNEIGTDEVLLHLLPVHQSEETDHRIRAYL
                     QAKLRVTPHIRYLTAEELQRLQFPVAGRKAMKFIDKRSHPGG"
BASE COUNT          782 a          860 c          816 g          900 t
ORIGIN      
        1 atcctcaaat atctctgatt taagtgcagc tgagaaccgt tccgaaccgg tggctacggc
       61 atgatgcgcc ccaccggttt ttaccgccat ataggcatat ttaaaggcat gcattccggc
      121 acaacatacg ccggagggag aaaccacttc aatggagcgg gattcgggca gctccccatg
      181 taccattacg gcatgggagg gcataatctg atcgggagaa gaagtaccgc agacgagcag
      241 gtcaatctcc ctgatctgtt ccgggtcatt gtccagcatg cggcggatgg cctctgccgt
      301 catttcagca ttggtatgag tgggtttccc ctcccgggtg agcgcgtaaa atctcctttt
      361 gatcccgtta ttacggagta cgattctttt tgatttggag gggcttttat tgatcaggcc
      421 caggaactcc tccatttcat cgttggatac gggttcgttg ggaaaatagg tagctgtttt
      481 tgtaatatag acctccttaa actccatctt catttccgga ttaattcact tctgaataat
      541 attgtttctg cctctttatg cgcccggata aaaatggttt gaaaaaaatg gcatctacag
      601 taaggataat cggggccgct ataaatagcg caattaaaag ataatatttg aatgcaacaa
      661 gccaggcagt tctttttttg cgcttggaaa taatgcctgc ccacacttta aaaatctttc
      721 tcgctttgga ctcaatgaac attaacggat acttgaccac cagccccccg gcatccacca
      781 gctcctcctg caacccttgc cagttgctgt ttttaagatg aggcagtaca atttccccaa
      841 atacttttgt atgaacaata tcggcgtcgg atacaccggg tttcggaaag atattcagat
      901 aacggtcctt tcttcccttg aacatccagt gaaataaggt aacgaaacta acgaaattgt
      961 gatggcggtc caccagggcg atattaccaa ccagtttggc tccaatattt cttaaggtag
     1021 gcttaatttt ttctatggcg cttatccaca tgttccgggc tccggtgatc gtgatcaccg
     1081 gcgtattccg ggccagggcc ctgaacttcg gatcattcag cagactgttg gccgggatgc
     1141 tgggagacag gaaccaggcc tggtatccca ggatgatcag gtcgtagaat tcctccttaa
     1201 aggaaatggg ttgaagcggc ccgggaacct ggttcacact atccggcatg accgaaaaaa
     1261 atgattcccc cgtccagggg aaagggtaag ggacgccggg ataaatgcgc agcttctcca
     1321 ccgatgcgcc tgcatccaaa agcggctggg tgaaatgatc tataatctct gctagctggc
     1381 cggactgggt atagaatacc gctaatactt ttttgcccat tctggggccg aagttaagaa
     1441 acctgcagct ttaaaatctt cgattttttg ctgtaatcct cgatgacccg ctgcagctcc
     1501 ccgggtaaag ttttatagga tttttcaatg atccggcggt ctcccggcaa atcgtcccgg
     1561 taccccagca cagcgggaga attttcctgt atgatcagcc tcaaaaaacg gtattccaca
     1621 ttattctggt ccctttttat agctgcttcc agctcatgcc ggccttgcct gaaaagtttc
     1681 aatttttggg gagggcttcc ccccaggccc gcctttttca tggttaaagc gccttcaaag
     1741 gcctgccgga gttcggccgg cgccattttt aatattttca gctgttcgtt caccaggtct
     1801 tttttgtcct gccgtatggc ctgataaaaa gcggtccggt caatgttccc ggcagattgg
     1861 gcaaatccgg ggctgctccc ggcgaccagg gccagcattc ccaaaaccca ttttattttt
     1921 ctcattgaag atttatattc atcccgttat catccaccgg gatggctcct tttatcaata
     1981 aatttcatcg ccttgcgacc ggcgaccgga aactgcaatc gctgcagttc ttcggccgtc
     2041 agataccgga tatgcggggt tacgcggagc ttggcctgca gataagcccg tatccggtgg
     2101 tccgtttcct ccgactggtg aaccggcagc aggtggagca gcacttcatc cgttccgatt
     2161 tcattggaat atacttcggc cacgaagtcc gtgatatcgt ccatcccgtt caacagatca
     2221 tataaagcgg gggcgaataa agtggtgccc ttgaatttga tcatttgttt tttccttccg
     2281 atcaccggcg acaaacgcat cgaaaaacga ccgcaggagc agggttcccg gtgcgcaata
     2341 cagatatccc ccgttttata ccgcagcaaa ggcatgcctt ccacatccag cgtcgtgatg
     2401 gtcacttccc cgggttctcc gtcgggtacg ggctgatcct gctcattcag cagttccgtg
     2461 ataaccagct ccggctgctg atggccccct ctcccggcgg aacattcggt gaatgccgtc
     2521 tgcatctccg tggaagcata ggtagaatac aattgaatgt tccatttctc tgttatcttt
     2581 cttcccaaaa cattcaggga aaaatcattt tgccggatat tctcaccgat gcaaatggct
     2641 tttcttaccg cgctggaacg gatatcgata tgataatccg aagcatactg gatcagtttg
     2701 aggataaagg agggaacagc cacgatggcc gaaggcctga gccggattat ggtttcccat
     2761 tgcagggagg gaacgccggg gccgacccgg ataataccgg cccccagttt ccggattccg
     2821 gagtaatacg ccatgcccgc catgaactga cggtcgaggg taagcatcag ctggtaaata
     2881 tcctccggtt ttccatccgc gcagtggaag gatccatact cgttgcgggc cagcctttgc
     2941 agatcttttt cagtcagcgc aaccgtgacc ggggctccca gcgtaccgga agtggaggta
     3001 tagtcaatga ccttcgtgcg gggcacacag agaaattcgt cgttgtattt ttgcaggtcc
     3061 tcctttaccg tcgtcggaag aagaccgagg tctcctaatc cccggatccg gttgagatca
     3121 attttttttt ccttaaatat tctctgataa tagggagaat gacccttgag gtgctgcaat
     3181 aattccagga gttttttttc ctgcgtccgg ttgatctctt ccggcttcat gaatatggtt
     3241 tgagcgggga tcatgattcc aatattacga cggctgtcgc atatgttttt aaatgcgaca
     3301 gcgaaacatg aataacccgg atctccaggg attcgagtcc tctggccgtt tcacccgt
//
LOCUS       DASYCU010000299         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2239128, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000299 DASYCU010000000
VERSION     DASYCU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239128"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGM24_06095"
     CDS             <1..489
                     /locus_tag="VGM24_06095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY4154974.1"
                     /translation="SKNPDIPYLLTKKQLLAELSETIIPATATPGAREAGVVDFMLPI
                     LTECTETRKLNNFIEGLQDLEAHTRYRYQKDFTQCSGKEKEEILLYFEKKAEPLTRFT
                     GKVKNKFLGGSFFNTLKQYSVECYCISEKGATGGLTYVPVPGRYEACIPLEPMQRAWA
                     TK"
     gene            496..2214
                     /locus_tag="VGM24_06100"
     CDS             496..2214
                     /locus_tag="VGM24_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4154975.1"
                     /translation="MGDPKPVYVNMKGLGQHRFDAIVVGSGISGGWAAKELCEKGLKT
                     LVIERGKNVVHIKDYTTAFTPPWQFPHGSNNTIQEIRDNPVASHNFNPGNSAFFVRDK
                     DHPYIQDDPFHWIRGYQVGGRSLTWGRQCYRLSDLDFEANAKEGYGVDWPIRYRDIEP
                     WYDYVEEFTGISGRPEGLPQLPDGRFLPPIEMNCIEDHFATAVHTHYKERIVTPARLA
                     NLTKGWKGRGPCQFRNLCDRGCPFSGYFSSNAATLPAAAATGNLSVVYDAVVIEILYD
                     DKKQRASGVRVMDSHTKSVTEYFSTVLFLNASTIPSAAILLQSKSGRFPQGLGNDSQE
                     AGHNLMDHFTGTGAFADYPGMEDKYYYGRKPGGIYMPRFRNINAETKRSDYVRGWGFQ
                     GSGSRKGWDSAAAELKGFGKDFKEKMLKPGPWTIWLGGWGETLPYHENRISLDDSKKD
                     QWGLPLLHIHFKYGDNEKAMKKDIRQTAVDMLEKTGFSNIEGFQYDPPAGSAVHEMGT
                     LRMGRDPKTSVLNGNNQVHSVKNLFITDGSCMTSSAWQNPSLTYMALTARACDFAVNE
                     LKKGNL"
     gene            complement(2280..>3082)
                     /gene="prmC"
                     /locus_tag="VGM24_06105"
     CDS             complement(2280..>3082)
                     /gene="prmC"
                     /locus_tag="VGM24_06105"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY4154976.1"
                     /translation="DEREASSISSLVMERITGMSKSLRVIHKKEALDENGSGLFRKYG
                     EELQQHRPVQYVLGEAWFAGLRFYVDENVLIPRPETEELLDWVMADLGTSSPSGFRVL
                     DIGTGSGCIPVSLGKRIPGIQLLACDIDGAALEVARKNSVENQVPVDFIQCDMLDESS
                     WNQIPGINLIISNPPYIPEKQKALLDPHVRLFEPGLALFVPDGDPLLFYKMIGEFAKK
                     KLLRGGKIFLEIHHDFSAQIFLWYRENGFIAELRKDLSGKNRMLRVQI"
BASE COUNT          797 a          785 c          799 g          701 t
ORIGIN      
        1 tccaaaaatc cggacatccc ttatctgctc actaaaaaac aactgcttgc ggaactgtcg
       61 gaaaccatta tcccggcaac cgctaccccg ggtgcccggg aagccggcgt ggtggatttt
      121 atgctcccca tcctgaccga atgcacggaa accaggaaac tcaataactt cattgaaggc
      181 ctgcaggacc tggaggcgca tacccgttac cgttaccaga aggattttac ccagtgttcc
      241 ggaaaagaaa aagaagagat cctcctgtat tttgaaaaaa aggcggagcc gctgacccgg
      301 ttcaccggca aggtaaaaaa caagtttctc ggaggcagtt tttttaatac cctgaaacag
      361 tattcggtgg aatgttactg catttctgaa aaaggggcca ccgggggcct tacctatgtt
      421 ccggtgcccg gaaggtatga agcgtgcatt ccgcttgaac ccatgcaaag agcctgggca
      481 accaaatagt aactcatggg cgatccgaaa ccggtttatg taaatatgaa agggctcggg
      541 cagcatcggt ttgacgcgat tgttgtgggc tcgggcatca gtggcggctg ggcagccaaa
      601 gaactctgcg aaaaaggcct gaagaccctg gtgatcgaaa gaggaaaaaa tgtggtccat
      661 atcaaagact atacgaccgc ttttacgccg ccctggcaat tcccgcacgg gtcgaacaat
      721 acaattcagg agatccggga caacccggtg gcctcccata atttcaatcc cggaaacagc
      781 gctttttttg tgcgcgataa ggaccatccc tatatccagg atgatccttt tcactggatt
      841 cgcgggtacc aggtcggcgg cagatcctta acctggggac ggcagtgtta ccggctcagc
      901 gatctggact ttgaagcgaa tgcaaaagaa gggtatgggg tggactggcc gatccgttac
      961 cgggacattg agccctggta cgactatgtg gaagaattta ccggcatcag cggaaggcct
     1021 gaaggacttc cccagcttcc ggacggaaga tttctgccgc caatcgaaat gaattgtatt
     1081 gaagaccact tcgctaccgc agtgcataca cattataaag aacggattgt tacgccggcc
     1141 cggctcgcca acctgaccaa aggatggaag ggccgcggtc cctgccagtt ccggaatctt
     1201 tgcgaccggg gatgtccctt tagcggatat ttctccagca atgcggccac gttgccggcg
     1261 gcggcggcga ccggaaacct ttcggttgta tatgatgcgg tcgttattga aatattgtac
     1321 gacgataaaa aacaacgggc atccggagtc cgggtaatgg attcccatac caaatcggtg
     1381 accgaatatt tttctactgt tctctttctg aatgcttcca cgattccgtc tgcggccatc
     1441 ctgctgcaat cgaaatcggg ccggtttccc cagggattgg gaaacgacag ccaggaagcc
     1501 ggccacaacc tgatggatca ttttaccgga accggcgcct ttgccgacta ccccggtatg
     1561 gaagataaat actattacgg acgcaaaccc ggaggaatct atatgcccag gttccggaat
     1621 atcaatgcgg aaacaaaacg ttccgattat gtgcgtggct ggggtttcca gggcagcgga
     1681 tccaggaagg gctgggattc ggcggccgcg gaactgaaag gattcggtaa ggattttaag
     1741 gagaaaatgt taaaacccgg tccctggacc atttggttgg gcggatgggg cgaaaccctt
     1801 ccttatcatg agaaccggat cagtctggat gattcaaaaa aagaccagtg gggattgccg
     1861 ctgctgcata ttcattttaa atatggcgat aatgaaaagg caatgaagaa ggatatccgg
     1921 caaacggctg tggatatgct ggagaaaacg ggcttttcca atattgaagg gtttcaatat
     1981 gatccgcctg ccggatcggc cgtacatgaa atgggcacgc tgcgcatggg acgggatcca
     2041 aaaacttccg tattgaacgg gaataaccag gttcatagcg tgaaaaatct ttttattacc
     2101 gacggcagtt gtatgacttc ttccgcctgg caaaatccat ccctgaccta tatggcactc
     2161 acggccaggg cctgcgattt tgcagtgaat gaattaaaga aaggaaatct ctgaacggaa
     2221 cgggcacccc cggtcatggt tatacgccgg atgccggggc cttccccgcc ggaaatggat
     2281 cagatctgaa ccctgagcat cctgtttttc cccgaaaggt cttttctgag ttctgcgata
     2341 aacccgtttt ccctgtacca taaaaagatc tgcgcggaga aatcgtgatg gatttccaga
     2401 aagatctttc ctccccgcag caatttcttt tttgcaaatt ctccgatcat tttataaaaa
     2461 agaagcggat cgccatccgg tacaaaaagc gcgagcccgg gttcaaaaag gcggacatgc
     2521 gggtccagca gtgccttctg tttttcggga atatagggtg ggttgctaat gatcaggttg
     2581 atgccgggaa tctggttcca ggacgactca tccagcatat cacattgtat aaagtcaacc
     2641 ggaacctgat tttccacact gtttttccgg gcaacctcca gggcagcacc gtcaatatca
     2701 caggccagca actgaatccc cggtatccgc ttacccaggc tgacgggaat acaaccactt
     2761 ccggttccga tgtccaggac cctgaagccg gaaggggatg aagtcccgag atccgccatc
     2821 acccaatcca gtaattcttc cgtctccgga cggggaatca gcacgttttc atccacgtag
     2881 aaacggaggc cggcaaacca ggcttcaccc aatacatact ggacgggccg gtgctgctgg
     2941 agttcttccc catatttccg gaataaaccg gaaccatttt catccagcgc ctccttttta
     3001 tgaatcaccc gcaggctttt gctcataccg gtaattcttt ccatgaccag ggagctaatg
     3061 gaagaagctt cccgttcatc gt
//
LOCUS       DASYCU010000300         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1516216, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000300 DASYCU010000000
VERSION     DASYCU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516216"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..2089)
                     /locus_tag="VGM24_06110"
     CDS             complement(344..2089)
                     /locus_tag="VGM24_06110"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY4154977.1"
                     /translation="MHKKESGPGSAEGLNWFKREFNIFTGKAITLKELLEQLRPNDSA
                     STKKAIEALKDCRLQYKRFAFFLEYFFPDEAFIFNGPPVQEIDPFQDEVIDPAGLQLI
                     ESLLYEGNAYRNREELVKQADLLVRSSEIFPLSLNDFKTQGPEVLTSIHLELVRIMTL
                     YITGYDAPRIKTGIVEAHESLSAIDSALAIYTENDPHRDSIRYYMGAGEKYLDAHPDF
                     DSFDRLAFITRYAQPLEKYLNQLIRKSAPDPNSFTALNPRADNLFTAAALDKNAFPHS
                     ENEKDTAIAGLGKRLFFESFLSGGRSRSCASCHSPAAYFTDGMPRNRSLDGKSDLPRN
                     TPSLFYAGYQYNQFWDGRVGSLDEQINAVLNNKSEMNSSADTITKRLERVPGYVTAFK
                     KIWPTDSAIGFNRVAGALAAYIRNLAPFRSAFDRYMQGDRSALSLNQQKGFNLFMGKA
                     QCGTCHFAPLFNGLLPPQYKTTEFEILGTPADDHLEKAKADSDQGLFTLFPDPHNKGA
                     FKTPTVRNAAVTPPYMHNGGFSTLEKVIDFYDKGGGAGLGLNVPEQTLSSIPLHLSDQ
                     EKTDLVAFIQSLTDK"
     gene            2389..>2857
                     /locus_tag="VGM24_06115"
     CDS             2389..>2857
                     /locus_tag="VGM24_06115"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEY4154978.1"
                     /translation="MKQQFRFLFFVLLLGSCTRQGNKENKITAENRLPGTTDWLIQVK
                     FDTCALPDHRYCRRPQIEGYCSQTSVKSGDSLNLYVSTNPASRFTIDIYRMGYYQGKG
                     ANLKKSIGPLQGKEQITAEPDPKTNFLECKWDKTYQLVIPPDWLSGVYLCKLTT"
BASE COUNT          757 a          679 c          676 g          745 t
ORIGIN      
        1 cagtatgttg tcaggttttt aaatatttgt aaatgttttt acctatattt tgtttacaaa
       61 ttgttaggca atctagataa ctttttaatt ataataaaga attcctggac atatttttaa
      121 gatttcagaa taatagtcaa ccggttagcc tggttaacca ccgccgaaac accccccggg
      181 aaagggttcc aatatgccgg ggaagtgatt tacaggagag aatggtttgg caaacctgct
      241 ccggcttaaa atatttttgt ttacaaataa tgggaaacaa tggtcctccg gcccgggcta
      301 cggttatgag tatctccggt gatgaccgga aatgaccgga actttatttg tccgtgagac
      361 tttggatgaa agcgaccaga tccgtttttt cctggtcact taagtgcagg ggaatacttg
      421 aaagcgtctg ctcaggcacg tttaagccta atccggctcc tccccccttg tcataaaaat
      481 cgattacttt ttccagcgtg ctgaatccgc cattatgcat ataaggtggc gtaactgcgg
      541 catttctgac cgtaggcgtc ttaaatgctc ccttattatg cggatccgga aagagtgtaa
      601 agaggccctg atcgctgtcc gccttcgctt tttccaaatg atcgtcagcc ggcgtaccga
      661 gaatttcgaa ttcggttgtc ttatactgcg gaggaaggag cccgttaaat aaaggagcga
      721 aatgacaggt tccgcattgg gccttgccca tgaacagatt aaaccctttt tgctggttca
      781 gagacagagc ggagcgatcg ccctgcatat accggtcgaa cgcagaccgg aaaggagcca
      841 aattacgaat ataagcagcg agcgcgccgg cgacccggtt aaagccgatc gccgaatcag
      901 tgggccagat ttttttgaat gcagtgacgt aaccgggaac cctttccagt cttttcgtga
      961 tggtgtccgc agacgaattc atttccgatt tattgttcag caccgcattg atctgttcat
     1021 ccaggcttcc cacgcggcca tcccagaatt ggttatactg atacccggca taaaataaac
     1081 tgggcgtatt cctcggtaaa tcgcttttgc cgtccaggct cctgtttcgt ggcatcccat
     1141 ccgtaaaata agcagccggc gaatggcagc tggcgcatga cctgctcctg cccccggaca
     1201 ggaagctttc aaagaacaag cgtttcccga gcccggcaat cgccgtatct ttctcattct
     1261 cggaatgcgg aaaggcgttt ttatccaggg ctgcggccgt aaataaatta tccgcccgcg
     1321 ggttgagggc agtgaaactg tttggatcgg gggcggattt gcggatgagc tgattcagat
     1381 atttttccag cggttgcgca taacgggtaa taaatgcaag ccggtcgaag gaatcaaaat
     1441 caggatgggc atccaggtat ttttctccgg cacccatata atagcggata ctgtctctgt
     1501 gtggatcatt ctccgtataa atggccaggg cggaatcaat ggcagaaaga gattcatggg
     1561 cctcaacgat tccggtcttg atccggggcg catcatagcc ggtgatgtac agagtcatga
     1621 tccggaccag ctccaggtga atgctcgtga gcacttccgg accctgcgtt ttaaaatcat
     1681 tcagtgaaag gggaaagatt tcagacgatc tcaccagcag gtccgcctgt tttaccagtt
     1741 cttccctgtt gcgataggca ttcccttcat ataataagga ctcaatcaat tgcagtcccg
     1801 ccggatcaat cacctcatcc tggaatggat caatctcctg gaccgggggg ccgttgaaaa
     1861 taaaggcttc atccggaaaa aaatattcca ggaaaaaagc aaaccgcttg tattgcaggc
     1921 ggcaatcctt aagcgcctca atcgcttttt ttgtggaagc ggagtcgttg gggcggagtt
     1981 gttccagcaa ttcttttaaa gtgatcgcct tgccggtaaa aatattgaat tcccgcttga
     2041 accagttcag gccctccgct gaccccgggc cggattcttt tttgtgcaac cgcggaattg
     2101 cggtggatac aatcatgaaa aagaccaaca atgtcaaaca gattttcata tcctggtttt
     2161 atccggcaat tccggttgcc ggttgaaaga aagaatccca taccggacac ccgttcacaa
     2221 aagatatttt aaaaaaaccc tgtaaaaata tatttatgtt tacaatttaa gattataatt
     2281 gtaaactatt tttaatcgaa cagtataatt tcacgatttc ctgtataaag accggttggc
     2341 ctgaataacc cgtctgaaac gggatttttt catctattta tccatcacat gaaacagcag
     2401 ttccgctttc tcttcttcgt tcttttactt ggatcctgca cccggcaggg taataaggaa
     2461 aataaaatta cggcagaaaa ccggttgccc ggaacaacag actggctgat ccaggtcaag
     2521 ttcgatacct gcgcccttcc ggatcaccgg tattgccgga ggccccagat agagggctat
     2581 tgctcccaga ccagcgtcaa aagcggagac agcctgaacc tgtatgtcag caccaatcct
     2641 gcttcgcgat ttacgatcga tatttaccgt atgggatatt accaggggaa aggggctaac
     2701 ctgaaaaaaa gcatcggacc cctccaggga aaggaacaga tcacggccga accggaccca
     2761 aagacgaatt ttctggaatg caaatgggac aaaacttacc agctcgtgat tcctccggac
     2821 tggttaagcg gggtatacct ttgcaaactg accacct
//
LOCUS       DASYCU010000301         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4991119, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000301 DASYCU010000000
VERSION     DASYCU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4991119"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VGM24_06120"
     CDS             <1..1156
                     /locus_tag="VGM24_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEY4154979.1"
                     /translation="TSVPYDSQDKIYADFIRRLQEAVAVLKTHAGENHYGTYDLIYGG
                     DVDRWVKFGNSLLLRIAIRISKVDPTTAKATAEAAVASGVMTTSIGASDPSAMTSGDD
                     AFVLRSAKNTDGNGLQSMSDWNEFRMSATMASVLNGFQDPRLPTYFLPTYNSGTLYKG
                     LRNGLSVDELGETPNLAANNSHVGKRWSSTAVLDKNNNVLGSATSVATAQNVMVTAES
                     YFLRAEGALLGWNMGGTAKDLYAAGITNSFAQWGITDNTVVQNYINSTNTPVAPGDGL
                     ASPAVSSVQVKFSADPAVQLEQIMTQKWLALYPDGMEAWADWRRSHVMKLYPVVHSDN
                     PDITNTSTQWIRRIPFLLSEEQNNEAAVEDAVGLLGGPDKVTTPLWWDKN"
     gene            1250..>4360
                     /locus_tag="VGM24_06125"
     CDS             1250..>4360
                     /locus_tag="VGM24_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY4154980.1"
                     /translation="MTNFRKCIPVILSAILFSACGEDHSRQFTRLDEEKTGIEFKNQL
                     FESDAFNVMNYIYFYNGGGVSIGDINNDGLPDILFTGNMVKNRLYLNKGNFKFEDITA
                     KSGIAEKQGWCTGATMVDINGDGKLDIYICRSADSKGSMRENLLFINNGDLTFTEKAA
                     EYGLADKGYSTQALFFDYDKDGDLDMFLINHSLHQYTTGAIENTGWRQQRNPDFACKL
                     FRNDWNDSLHHPVYTDVSLQAGINSSVLTFGLGVVASDINNDGWPDLYVSNDFNEPDY
                     LFINNRNGTFSEKLHDCMDEVSLYSMGCDVADFNNDGLVDVLTLDMLPEDNRTQKMHS
                     GSENFDKFQMLFNKGFYYQYSRNMLQKNNGDGTFSEVGQLAGVSNTDWSWSALFSDFD
                     NDGNKDIFISNGYVKDYTDMDFIKYSLDNSLHSGENGGKSTVEDYIKKMPDNILPNYI
                     FQNQGDLHFVKKSKDWGIDQNRVSAGAAYADLDNDGDMDLVINNTNDFAGIYENNNER
                     IDKTNWLRIKLQGDARNETGIGSKVKVFCQGREYFQEAFPVRGFQSSVDPVLSFGLGS
                     HGQADSVIVIWPNDQFQKFTQVKANQTLAVKLAEATGKWIYDSAINKKQAYFSAVPAP
                     AGMQHHGRNFNDFSLQKLMTGYLSKTGPVMAVADVNKDGREDVFFGGGRDQPSRLFIQ
                     NRQGTFTLSPQPAFLKDSAFAAGSALFFDADQDGDMDLYLASSGYDLDQHSSYLEDRL
                     YLNDGKGNFTRSPNPLPPMPFSKSSVKAADVDGDGDMDLFIGGRVIPGKYPLSPGSRI
                     LLNDGKGNFTDATSSTAPVLRNLGMVTDAVWTDLNGDKKPDLVVVGEYMPVKVFINKN
                     GKLSDESASYIPFESSGWWNTVYAADMDGDGDQDLVIGNMGLNTPFHASAKEPVSLYY
                     KDFDGNGSVDPIFCYYIDGVSYPAISRDDLTDQIPALKKKFLKYSDYSTATINDLFTP
                     EQLKGAGLLKAGEMHTVYLENKENKSFVLHELPVEAQYSPVYAITSIDINKDGKKDLV
                     LAGNNSW"
BASE COUNT         1187 a         1020 c         1101 g         1052 t
ORIGIN      
        1 cacctctgtc ccgtacgatt cccaggataa gatctatgcc gactttatca gaaggttgca
       61 ggaagccgtc gccgttctga agacccatgc cggtgaaaac cattatggca cgtacgattt
      121 gatttatggg ggtgacgttg accgctgggt taaattcggc aattcactgc tgctgcgaat
      181 cgcaatccgg atctcgaaag tggatccgac cacggccaaa gcaacggctg aagccgcggt
      241 ggcgagcggg gtgatgacca ccagtatagg agcttctgat ccttctgcga tgacctccgg
      301 cgacgatgcc tttgtgctga gaagtgcgaa gaacacagat ggaaacggac tccaatccat
      361 gtcggactgg aatgaatttc ggatgagcgc taccatggcg tctgtactca acggattcca
      421 ggatccgcgt cttccaacct attttttgcc gacttataat tccggtacac tgtacaaagg
      481 gctccggaac ggcctttcag tcgatgagct cggagaaaca ccaaacctgg ctgccaacaa
      541 ctctcatgtc ggcaagcgct ggtcttccac tgcggtgctg gacaagaaca ataatgtact
      601 tggttctgcc acttcagttg ccactgcgca aaatgttatg gtgaccgcag aatcttattt
      661 cctgcgggcg gaaggcgccc tgctgggctg gaatatgggg ggaacggcga aagatcttta
      721 tgcagccggc attaccaatt cctttgccca atggggtatt accgataata cggtcgtaca
      781 gaattatatc aatagtacca acacaccggt ggctccgggc gatgggctgg cctcccctgc
      841 cgtaagttcc gttcaggtta agttttcggc tgatccggcc gttcagctgg aacagatcat
      901 gacccagaaa tggctcgccc tttatcccga tggtatggaa gcctgggccg actggcggag
      961 aagccacgta atgaaacttt atccggttgt tcattccgat aacccggata ttaccaatac
     1021 ctccacccag tggatccggc gcattccgtt cctgctgtcc gaagaacaga acaacgaagc
     1081 tgccgtggaa gatgccgtag gtctgcttgg cggtccggat aaagtgacca ccccgctctg
     1141 gtgggataag aattagtatc tgattttttt ataccttaag attccatcat tcatttgatg
     1201 gaatcttttt gttttagcca atccgtttat ccgttcaaaa agaaatatta tgaccaactt
     1261 ccgcaaatgc ataccggtga ttttatccgc cattttattt tcggcatgcg gcgaagatca
     1321 ctcccgccag tttacccggc tggacgaaga aaagaccggc attgaattca aaaaccagtt
     1381 gtttgaaagc gatgcattca acgtgatgaa ctatatctat ttctacaacg gcggcggtgt
     1441 ttccatcgga gatattaata atgacggtct tcccgatatc ctgtttaccg gcaatatggt
     1501 caaaaaccgg ctctatctca acaaaggaaa ttttaagttt gaagacatca ccgccaaaag
     1561 cgggattgcg gaaaaacaag gctggtgtac gggcgcaacc atggtggata ttaacgggga
     1621 cggcaaactg gacatctata tctgccgctc ggcagacagc aaggggtcca tgcgggaaaa
     1681 cctgctgttc atcaataacg gcgatttaac ttttacggaa aaggcggccg aatacggact
     1741 ggcagataaa ggctacagta cccaggcctt gttttttgac tatgataagg atggagacct
     1801 ggatatgttc ctgatcaatc attccctgca ccagtacact accggggcaa ttgaaaatac
     1861 cggatggcgc caacagagaa atcccgattt cgcctgcaaa ttattccgga acgactggaa
     1921 cgacagcctg caccatcctg tatatacgga tgtctccctc caggcgggca taaattcgag
     1981 cgtcctcacg ttcgggttgg gagtcgtggc ttcggatatc aataacgacg gctggccgga
     2041 tttatatgtt tccaacgatt ttaatgagcc ggattatctt ttcatcaaca accggaatgg
     2101 aacgttcagt gaaaaactcc atgactgcat ggacgaagtc tccttatact ccatgggctg
     2161 cgatgtggca gattttaata acgacggact ggttgatgtg ttaaccctgg acatgcttcc
     2221 ggaagataac cggacacaga aaatgcacag cggttcggaa aacttcgata aattccagat
     2281 gctttttaat aagggttttt attaccagta cagccggaac atgctgcaaa aaaataacgg
     2341 ggacggcacg ttcagcgaag tgggtcagct ggcaggggta tccaataccg actggagctg
     2401 gtcggcactt ttctccgatt ttgacaacga cggcaacaaa gatattttta taagcaacgg
     2461 gtatgtgaag gattatacgg atatggattt tatcaagtac agcctggata attcccttca
     2521 ttccggagag aatggcggaa agtccactgt cgaagactat ataaaaaaaa tgccggacaa
     2581 catcctgcca aattatattt ttcagaacca gggtgacctt cattttgtaa aaaaatccaa
     2641 agactgggga atagatcaaa accgggtttc agccggagct gcctatgcag acctggacaa
     2701 tgacggggat atggatctcg tgatcaataa taccaatgac tttgccggca tttatgaaaa
     2761 caataatgaa cgaattgaca aaaccaactg gctccgcata aagctgcagg gcgatgccag
     2821 gaatgaaacg ggcatcggat ccaaagtgaa agtattttgc cagggccggg aatatttcca
     2881 ggaagcattt ccggtccggg gattccaatc ttccgttgac cccgttttaa gtttcggtct
     2941 gggcagtcac gggcaggctg actccgtaat cgtgatctgg ccaaacgatc agttccagaa
     3001 attcacccag gtaaaggcta accaaaccct tgcggttaaa ttggcggaag ccaccggcaa
     3061 atggatttat gattccgcaa taaacaaaaa gcaggcttat ttttctgccg ttcctgcacc
     3121 ggccggaatg cagcatcatg gaaggaattt taatgatttt tcattacaga aactgatgac
     3181 cggctatctc tctaaaacag gtcccgtcat ggcggtggct gatgtaaata aagacggcag
     3241 ggaggatgtt ttctttggtg gcggccggga tcagccttcc cgcctcttta tacaaaaccg
     3301 gcagggaacg tttacgctct ccccccagcc tgctttcctg aaagattccg ccttcgccgc
     3361 cgggtcggct cttttctttg atgcggatca ggatggggat atggacctgt acctggccag
     3421 cagcggatat gacctggatc agcacagcag ctatctggaa gaccgtttat atctgaatga
     3481 cgggaaagga aattttaccc gcagccctaa tccccttccc ccgatgcctt tcagcaagag
     3541 cagtgtaaaa gctgcggacg ttgatggaga cggggacatg gatttattta tcgggggccg
     3601 ggtaattccc ggaaaatatc cgttatcacc gggcagccgt attttactga atgacgggaa
     3661 agggaatttt accgatgcta cctcatccac tgcgccggtg ctcaggaatc tgggaatggt
     3721 aacggatgcc gtatggacgg atctcaatgg tgataagaaa ccggacctgg tcgtagtcgg
     3781 ggagtatatg ccggtaaaag tattcattaa caaaaatgga aaattatccg atgaatccgc
     3841 cagctacatc cccttcgaaa gcagtggctg gtggaacacg gtttatgctg cagacatgga
     3901 cggggacggg gaccaggacc tggttattgg caatatgggt ttgaacaccc catttcacgc
     3961 gagtgcgaag gaaccggtaa gcctgtacta taaagacttt gatggaaacg gatcagtgga
     4021 tccgatattt tgctattata tagatggggt atcttaccca gccatatcca gggacgatct
     4081 gacggaccag ataccggcat taaaaaagaa gttcctgaaa tattccgact acagcacagc
     4141 aacaataaac gatctgttta cgccggaaca attgaaaggg gccggtttat taaaagcggg
     4201 agagatgcac acggtctacc tggaaaataa agagaataaa agttttgtat tgcatgaact
     4261 cccggtggaa gcgcaatatt ctcccgtgta tgccatcacg tcaatcgata tcaataagga
     4321 cggcaagaaa gacctggtgc tggcaggaaa caattcctgg
//
LOCUS       DASYCU010000302         5626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_210768, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000302 DASYCU010000000
VERSION     DASYCU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210768"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VGM24_06130"
     CDS             complement(<1..863)
                     /locus_tag="VGM24_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4154981.1"
                     /translation="MRVSSKENFQVAFSRIARLKKFLPVGSLAILAIAFSLPGYAQNT
                     IHVTGNIKNESGQPVSGASVLVKGLRTGVTSQENGHFEIDAPTKGTLVVSYVGMVTQE
                     LNVGASPITVTLVSLETGLNQVVVVGYGSQKKSDITGSVVNVSSRTLQETPTANIISE
                     LKGRAAGVDIVSNSSYPGSSGQIRIRGNRSLVQEITGNPSATAANNDALNGPLLVVDG
                     IPYGGSVNDLNPDDISGIDILKDASATAIYGSRGSNGVLIITTNRGKSGKAVMSYNGY
                     FGLTNALGEYKG"
     gene            1228..2271
                     /locus_tag="VGM24_06135"
     CDS             1228..2271
                     /locus_tag="VGM24_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4154982.1"
                     /translation="MNNGKEVTMYDLARKLNISVATVSRGLQDDPVVSKKTKKKITEL
                     AEELGYRSNNFARNLRTRRTQTIGVIVPRLKSYFMSTVIAGIESVANDAGYNLIISQS
                     SESADKEKASAKTMFNNRVDGLLVSLAYDTEDLSHLEAFTRKNIPVLFFDRVADHRDF
                     TNILIDNKKAAYEATSHLISQGCRKIIHITAPPKQNVYIDRLEGYKKALAEHRMRFLE
                     DNVIIGNLSQEAGAEAAEVILKMKPLPDAVFAANDNCAVGCMLALKQAGIRIPEQIAF
                     VGFNNDPVSKVIEPNLTTINYPGYEMGEVAARNLINHLDGVSALHSTNTILLRSEFVE
                     RESSQKQGRIHHN"
     gene            2293..3045
                     /locus_tag="VGM24_06140"
     CDS             2293..3045
                     /locus_tag="VGM24_06140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4154983.1"
                     /translation="MENKKKQPLALVTGGGSGIGLAIARKFTENGIRTLILGRDAGKL
                     SSAKKNLGPLCEEVRFDLDRLDALPEMVNEITDRFGTINILVNNAGVNLKKEFTEVTD
                     GEFQQVLLTNVAAVFALSRELVKKMMEAGNGSIIHISSMASQYGIPKVIAYSASKSAI
                     EGMTRSMAVDLSPHGIRVNCIAPGFIETEMSSKALNGDQERMQKVLSRTPMGKLGKPE
                     DIAEAALFLASDASRFITGVVLPVDGGNSIGF"
     gene            3071..>5626
                     /locus_tag="VGM24_06145"
     CDS             3071..>5626
                     /locus_tag="VGM24_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HEY4154984.1"
                     /translation="MKRIALIFSCLLSGGLAYCQPHVSENRTAGSFPLVAGGTAATLC
                     TDDGDYWLVNKSAALFQQDIERVTGIKPALVHRLPAAAKQIILIGSLDHSSLIRQLVE
                     KNKIGTESIKGKWECFRIQTVEHPFPGIDRALVIAGSDRRGTAFGVFEISRQMGVSPW
                     YWWADVPVKKKKECYVDKNGMVSGPPSVKYRGIFINDEAPAFSGWTREKFGGVNHGVY
                     EKIFELLLRLKANYLWPAMWGNAFNDDDTLNPILASKYGIVMGTSHHEPMLRAQQEWK
                     RYGKGQWNYDSNAAVLRDFWRKGIEKMDSHESIVTIGMRGDGDMPMTEGSNIALLERI
                     VHDQRQIIQQVTGRDPSETPQSWALYKEVQDYYDKGMRVPDDVTLLLCDDNWGNIRKL
                     PKRTDPPRAGGYGIYYHFDYVGDPRNYKWLNTNNIARVWEQMHLADAYGANRIWIVNV
                     GDLKPMEYPISFFLDYAWNTKKWNAGNLDDYPLQWVQEQFGKRYAKDIAQLLKKYSGY
                     NSRRKPELLSPDVYSLVHYREAETVLQDYYKLQADAEKIYEKLPAASRDAFFELVLYP
                     IKSCANLNALYITTAKNRMYASQGGSETNMLADSVAKLFEKDSILSHEYNTLAGGKWD
                     HMMDQTHIGYTYWQQPPVQTIPATRSIPVPEKASMGVSIDGSPSAWPGEQEKARLPAI
                     SRSNKSTYIELFNRGSLPYAYSIHTDAPWLLVSPAKGRVGYLQRIELRADWTKVPNGK
                     AEAMLRVEGPDGQAVSVMVPVENLIPRSFTGFPETNGYVSIQADHYSKAVNEGAVHWE
                     LIPGIGKSGNGMHIDPVTAPAALAGKGPYLEYPVWLSDTGRILVNVSCSPTLGF"
BASE COUNT         1450 a         1450 c         1446 g         1280 t
ORIGIN      
        1 cctttgtatt ctcccagggc gttcgtcaac ccgaaatatc cgttgtaact cataacggct
       61 ttccccgatt ttcccctgtt cgttgtgatg atgagcactc cattggatcc tctcgatccg
      121 taaatggcgg ttgccgacgc atcctttaat atgtcaatac cggaaatatc gtcggggttg
      181 agatcgttaa cgcttcctcc gtacggaatg ccgtcaacca cgagcagagg tccgttcaat
      241 gcatcattat tggccgccgt ggctgaagga tttccggtaa tttcctgcac cagggagcgg
      301 ttgccccgga tgcggatctg cccgctggaa cccggatagg aactattgct gacaatatcc
      361 acgccggccg cccttccttt cagttcactg atgatatttg cggtgggtgt ttcctgcagg
      421 gtgcggctgc tgacgttaac cacagacccc gtgatatcgc tttttttctg gcttccgtat
      481 ccaaccacca ccacctggtt caaaccggtc tcgaggctga ccagcgtaac cgttatgggg
      541 gacgcaccca cattcagttc ctgggtgacc attcccacat aggaaacgac cagggttcct
      601 ttcgtcgggg cgtctatttc aaaatggccg ttctcctggc ttgtaacccc tgttcgcaat
      661 ccctttacca gcaccgatgc cccggatacc ggctgaccgc tttcattctt tatatttccc
      721 gttacatgaa tcgtgttctg cgcatatccg ggcaatgaaa aggctattgc cagaatggcc
      781 agtgatccga ccggaagaaa ttttttcaac ctggcgatcc tgctgaaggc cacctgaaaa
      841 ttttcttttg atgaaactct cataactgag ctttttttta aatagtgaac gttttggtta
      901 acagaaatcc cgcccgatgc aggcggggcg cgcattcaga tctggtcagc cttgccgctg
      961 acggcaaacg ggagagaaag gggaacctca aaaaaaagaa atcagcaacg gaagaattcc
     1021 aatgtgaaga agccggaaac cggttggccg gaaaagcaga aattggtttt ggctgcatca
     1081 gacaatcgtt tttttttgag ttaggcaagt atgcagttcg gaatcgccgc actaaaatag
     1141 ggaaaaaaac aacaaaatgt aaccgattac attttatttc aaaaaatata tctaatttac
     1201 ttttatttcc ctcatgtaag cgtttgtatg aacaatggaa aagaagtaac catgtacgac
     1261 ctggcccgca agctgaatat ctccgtggcc acggtcagcc ggggtttgca ggatgacccg
     1321 gtggttagca aaaaaacaaa gaaaaagata acggaactgg cggaagaact ggggtaccgg
     1381 tccaataatt ttgcccggaa cctgcgcacc aggcgcacgc agaccatcgg cgtgatcgtt
     1441 ccccggctga agagttattt catgtcaacc gttattgccg gcattgaatc cgttgccaat
     1501 gacgccggat acaatctgat catatcgcaa tcctcggaat cggccgacaa ggaaaaagca
     1561 agtgcgaaaa ccatgttcaa caaccgggtc gacggcctgc tggtctctct tgcttacgat
     1621 acggaggacc tttcccacct ggaagccttc acccggaaaa atatccccgt gctgtttttt
     1681 gaccgggtcg ccgaccaccg ggatttcacc aatatattaa tagataataa gaaagcggcc
     1741 tatgaggcca ccagccacct gatcagccag ggctgccgga aaatcattca tattaccgcg
     1801 cctcccaaac aaaatgttta tattgaccgt ctggagggat acaagaaagc gctcgccgaa
     1861 caccggatgc gttttctgga ggacaatgtg atcatcggta atctcagcca ggaagccggg
     1921 gcggaagcgg cggaagtgat cctgaagatg aaaccgctgc cggacgccgt attcgcggcc
     1981 aatgataatt gcgcggtggg atgcatgctc gcccttaaac aggccggcat ccgcatcccg
     2041 gaacagattg cctttgtcgg atttaacaat gacccggtat ccaaggtaat cgaacccaat
     2101 ctgaccacca ttaattatcc gggctatgag atgggggaag tggctgcccg gaatctgatc
     2161 aatcacctgg acggagtttc ggcattgcac agtaccaata ccatcctact ccggtcagaa
     2221 tttgttgaac gggaatcttc tcaaaaacag ggaagaatcc atcacaatta aaccgcttat
     2281 acaactgctt gaatggaaaa taagaaaaaa caacccctcg cactcgttac cggcggagga
     2341 tcaggaattg ggctggcgat tgcccggaaa tttacagaaa atggaatccg cacgcttatc
     2401 ctgggccgcg atgccggtaa attaagttct gcaaaaaaaa atctcggtcc gctttgcgaa
     2461 gaagtccgtt tcgacctgga ccggctggat gcgcttcccg aaatggtgaa tgaaataacg
     2521 gaccgttttg gtaccataaa tattctcgtc aataatgccg gcgtaaatct gaaaaaagaa
     2581 tttacggaag tgaccgacgg ggaatttcag caggtattgc tgaccaatgt ggccgccgtg
     2641 tttgcccttt caagggaact ggtaaaaaaa atgatggaag ccggtaacgg ttccatcatc
     2701 catatcagct ccatggcttc ccaatatgga attccgaagg tgatcgccta cagcgcatcc
     2761 aaatcggcca tcgaaggcat gacccgttcc atggcggtgg acctttcccc gcacgggatc
     2821 cgggtaaact gtatcgcacc gggatttatc gagaccgaaa tgtcgtccaa agcactcaac
     2881 ggcgaccagg agagaatgca gaaagtttta tcccgcacgc ccatgggcaa actcggtaaa
     2941 ccggaagaca ttgcggaagc cgctttgttc ctcgcttcgg atgcttcccg tttcattacg
     3001 ggagtggtgc tcccggtaga tggcggaaat tccatcggat tctaacctgt tcatttcaaa
     3061 tgcgcttcag atgaaaagga ttgcccttat tttcagttgc ctgctctccg gcggcctcgc
     3121 ctattgtcag ccgcacgttt ctgaaaatag aacggccggc tcctttccgc tcgttgccgg
     3181 cggaaccgcc gccaccctct gtacggatga cggagattac tggctggtga ataagtcggc
     3241 cgcccttttt cagcaggata tcgaacgcgt gaccggaata aaaccggccc tagtacaccg
     3301 gcttcccgcc gccgcaaaac aaatcatcct catcggcagc ctggatcatt cctccctgat
     3361 ccgtcaactc gttgaaaaaa ataagatcgg cacggaatca ataaaaggaa aatgggaatg
     3421 ctttcgcatt caaacggttg aacatccgtt tcccggcatt gaccgcgccc tggtgatcgc
     3481 cggcagcgac cggcgcggaa cagccttcgg ggtttttgaa atttcccggc aaatgggtgt
     3541 atcgccctgg tactggtggg ccgatgttcc ggtgaaaaag aagaaagaat gctatgtaga
     3601 taaaaacggg atggtttcag gtccgccctc cgtaaaatac cgcggcattt ttatcaatga
     3661 cgaagcgccg gccttctcgg gctggaccag ggaaaagttc gggggcgtaa atcatggcgt
     3721 ttacgaaaag atattcgaac tgctgttaag actgaaagca aactatctct ggcccgccat
     3781 gtggggcaac gcttttaacg acgacgatac gctgaatccc atactggcct cgaaatacgg
     3841 catcgtcatg ggcacttccc atcacgaacc catgctccgg gcccagcagg agtggaagcg
     3901 gtacgggaag ggccagtgga attacgacag caatgccgcg gtgctgcgcg atttctggag
     3961 gaaagggata gaaaaaatgg actcgcatga aagcatcgtc accatcggga tgcggggaga
     4021 cggggacatg ccgatgacgg agggcagcaa tatcgctttg ctggagcgga ttgtgcatga
     4081 ccagcgtcag atcattcagc aggtcaccgg gagggaccct tccgaaaccc cacagtcctg
     4141 ggcgctctat aaggaagtgc aggactatta cgacaagggt atgcgggttc ccgacgatgt
     4201 gaccctgctt ctctgcgacg acaactgggg caatatccgg aaacttccga aaaggaccga
     4261 tccgccgaga gccggcgggt atggtatcta ttaccatttc gattatgtgg gtgatccccg
     4321 gaattataaa tggctaaata caaataatat cgcccgcgta tgggaacaga tgcacctggc
     4381 ggatgcgtat ggcgccaacc ggatctggat cgtgaatgta ggtgatctga agcccatgga
     4441 atatcctatc agcttttttc tggactatgc ctggaatacg aaaaaatgga atgccgggaa
     4501 cctggatgac tatccccttc aatgggtgca ggaacagttt ggaaaacgat atgccaaaga
     4561 catcgcgcag ctcctgaaaa aatattccgg atataacagc cggaggaaac cggaactgct
     4621 ttccccggat gtctatagcc tggttcatta ccgggaagcg gaaacggtgc tgcaggatta
     4681 ttataaactg caggctgatg cggaaaagat ttatgaaaaa ttgcctgccg cctccagaga
     4741 tgcctttttc gaattggtgc tttatcccat aaaatcctgt gctaacctga acgcattata
     4801 cattacgacg gctaaaaacc ggatgtatgc ctcacagggc ggttcggaaa ccaatatgct
     4861 ggcagacagc gtggcaaaac tctttgaaaa ggattctatc ctctcgcatg aatacaatac
     4921 cctcgccggg ggaaagtggg accatatgat ggatcagacg catatcggat atacctactg
     4981 gcagcagccg ccggtgcaga ccattccggc aacccgaagt attccggttc ccgaaaaagc
     5041 ttccatgggg gtctccatcg acggttcgcc ctcggcctgg ccgggtgaac aggagaaagc
     5101 gcgtttgcca gccatcagca gatcaaataa atccacctat atcgaattgt tcaaccgcgg
     5161 cagcctgccc tatgcgtaca gcattcatac agatgctccc tggctgctgg tttctcccgc
     5221 aaaaggcagg gtcggatacc tccagcgcat tgagttgcgc gccgactgga ccaaagtgcc
     5281 caatggaaag gccgaagcga tgctccgcgt tgaggggcct gacggtcagg cggtcagcgt
     5341 gatggtgcct gttgaaaatc tcatcccccg gtcgtttacc ggttttcccg aaaccaacgg
     5401 atatgtttcc attcaggcgg atcattattc gaaagccgtg aatgaaggcg cggttcactg
     5461 ggaactcata ccgggtatcg gaaaaagcgg aaacgggatg catatcgatc cggtgacggc
     5521 gcctgccgcc ctggccggta aaggccctta tcttgaatat cccgtctggc tttccgatac
     5581 aggtcgcata ttggtaaacg ttagctgctc ccctacgctg ggcttc
//
LOCUS       DASYCU010000303         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2675909, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000303 DASYCU010000000
VERSION     DASYCU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675909"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VGM24_06150"
     CDS             <1..200
                     /locus_tag="VGM24_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEY4154985.1"
                     /translation="NIYAIQGYETEILAASIRSSLHIVQCAEAGADVVTSPLEPILGL
                     LKHPLTDIGLAKFLADAKGAK"
     gene            395..1258
                     /gene="hisG"
                     /locus_tag="VGM24_06155"
     CDS             395..1258
                     /gene="hisG"
                     /locus_tag="VGM24_06155"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY4154986.1"
                     /translation="MSSVKLKIAIQKSGRLYDDSLKLLKDCGIDFKNGINKLKTEADN
                     FPMEVFFLRDDDIPQYVEDAVADIGIVGENVIYEKGREVLITEKLGFGKCRLSVAVGR
                     NEQYDGAEFLRDKKIATTYPVLVSGFLQKKKISADIHEISGSVEIAPGIGLADAIVDL
                     VSSGSTLFMNGLKEVETILQSEAVLIRHPELDAARQQILEKLLFRIRSVRKARHTKYI
                     LLNAPNAKLNEIISLLPGMKSPTVLPLAEAGWSSVHSVVNEDEFWDIIEKLKEAGAQG
                     ILVVPIEKMIL"
     gene            1277..2575
                     /gene="hisD"
                     /locus_tag="VGM24_06160"
     CDS             1277..2575
                     /gene="hisD"
                     /locus_tag="VGM24_06160"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY4154987.1"
                     /translation="MQIVEYPGRPEWEKLLARPEKDIASLEGLVKKIMEDVKLGGDRA
                     VDKYSLEFDKADLKDRRVPESEWESAGQELSGPLKAAIRQAKNNIERFHRTQFVQEEV
                     METMPGIRCWRKSVPIEKVGLYIPGGTAPLFSTILMLAVPAKIAGCREIVLCSPPGKD
                     GKLHPAILFAASLTGVRQIFKVGGAQAIAAMAYGTATVPKVDKIFGPGNQYVTCAKQL
                     LQLEGVAIDMPAGPSELAIYADETATPAFVAADLLSQCEHGPDSQVILVTGSRKIAEA
                     VIRETGLQLSVLPRKDIARKALENSRFFLVHDGEEAMELLNAYAPEHLILACEDAEGR
                     AAGVQQAGSVFIGHFAPETAGDYASGTNHTLPTNGYARAYSGVGVDSFLKKISFQQLS
                     ESGLSGISQTLIEMARAEGLQAHAAAVEIRLKQMNTPAHA"
     gene            2568..>3590
                     /gene="hisC"
                     /locus_tag="VGM24_06165"
     CDS             2568..>3590
                     /gene="hisC"
                     /locus_tag="VGM24_06165"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY4154988.1"
                     /translation="MPDINRLLRNNIKKLVPYSSARDEFTGTASIFLDANENSLGSPI
                     PEQYNRYPDPHQVKIKEMLSAIKGVPAKNIFLGNGSDECIDLLYRAFCNPGRDNVIIN
                     PPTYGMYEVSARINDVEIRKAVLTEDFQPDLEKMESLTDENTKMIWLCSPNNPTGNAF
                     HYQSIEMILHNFDGLLVIDEAYINFSRQQSFIRELTEFPNLVVLQTLSKAWGLAGLRM
                     GMAFASESIVEILNRIKPPYNISQPAQELVLKALKETGRVNERIREIVLMRDLLAAGL
                     GQVPGVKKVYPSDANFLLVKMDRAREVYETLLKQGIVVRDRSRVVLCDDCLRITVGKK
                     EENEELL"
BASE COUNT          954 a          857 c          990 g          789 t
ORIGIN      
        1 ccaatatcta tgccatccag ggttatgaaa cggagattct ggccgcttcc atccggagtt
       61 cactgcatat tgtccaatgc gcagaggccg gggctgatgt ggttacttcg ccgctggaac
      121 cgatcctcgg gttgttgaaa cacccgttaa cggatatcgg cctggccaag ttcctggccg
      181 acgccaaagg ggccaaatga gaataatttt ggcacaatgc ttccgggcaa ttatttttgc
      241 tccgcatcta tttatgatta tttctttatc catacatcat catcatacct tgcccaaccg
      301 gtaggctgct gatgttttgt gtaaacaata taattgcagg ggcttaccga aaggtaggcc
      361 cctgttcttt ttttgtatca ctgaaacgaa aaatatgtca tccgtcaaat taaagattgc
      421 cattcagaaa tcaggccggc tgtacgatga ttccctgaaa ctgctgaaag actgcggcat
      481 cgacttcaag aacgggatca acaaattaaa aacggaagcg gacaattttc cgatggaagt
      541 tttttttctg cgggatgacg acataccgca gtacgtggaa gatgccgttg ccgatatcgg
      601 tattgtgggt gaaaatgtga tttatgaaaa gggcagggag gtgctgataa cggaaaaact
      661 gggttttggc aaatgccgcc tgtccgttgc tgtaggaagg aacgaacaat atgacggagc
      721 ggaatttctc cgggataaaa aaattgccac gacgtatccc gtgctggttt ccggattcct
      781 ccagaagaaa aagatctcag ccgacatcca cgagatcagc ggttcggtcg agatcgctcc
      841 cggcattggc ctggccgacg cgatcgttga cctggtgagc agcggatcca cgctctttat
      901 gaacggattg aaagaagtgg aaacgatctt gcaatccgag gccgtgctca tccggcatcc
      961 ggagctggat gccgcaaggc agcagatcct ggaaaaattg ctgttccgga tccgttcggt
     1021 gcgaaaagcc cggcatacca agtacatcct gctcaacgcg ccgaatgcga aactgaatga
     1081 aattatttcc ctcctgccgg gcatgaaaag cccgacggta ttacccctgg ccgaagccgg
     1141 ctggagcagc gtgcacagcg tggtgaacga agatgaattc tgggacatca ttgagaaatt
     1201 aaaggaagcc ggcgcccagg gaatcctggt ggtgccgata gaaaaaatga ttttataata
     1261 ttaaacgcca tgatccatgc agatcgttga atatcccgga aggccggaat gggaaaaatt
     1321 gctggcgagg ccggagaaag atatagcttc cctggaaggg ctggtaaaaa agatcatgga
     1381 ggatgtgaaa ctgggcgggg accgggcagt ggataaatat tcactggaat tcgacaaagc
     1441 ggacctgaag gaccggcgcg tgccggaaag cgaatgggaa tcggccgggc aggaattatc
     1501 cggaccccta aaagcggcta tccggcaggc aaagaataat atcgaacgtt ttcacagaac
     1561 acaatttgtt caggaagaag taatggaaac catgcccggc atccgctgct ggaggaaatc
     1621 ggttcccatt gaaaaagtgg ggttgtatat accgggcgga acagctccgt tattctccac
     1681 gatactgatg ctggcggttc cggccaaaat tgccggttgc cgggaaatcg tgctgtgcag
     1741 ccctccgggt aaggatggca aactacatcc ggccatcctg tttgcagcgt ccctgacagg
     1801 cgtccgccag atctttaagg tcggtggagc gcaggctatc gccgcgatgg catacggaac
     1861 cgcaaccgta cccaaagtgg ataaaatttt tggaccgggc aaccaatacg tcacctgcgc
     1921 caaacaatta cttcagctgg aaggcgtcgc catcgatatg ccggccggcc cgagtgaact
     1981 cgccatatac gcagacgaaa cagccacacc cgcgtttgtt gcggcagatc ttttgtccca
     2041 gtgtgaacac ggaccagaca gccaggtcat cctggtaacc gggagcagga aaattgcgga
     2101 agcggtgatc cgggagaccg gcttacagct ttcggtactc cccagaaaag atatcgcccg
     2161 gaaggcattg gaaaacagcc ggttcttcct tgtacatgat ggagaggagg ccatggaatt
     2221 gttaaatgcc tacgctccgg aacatttgat cctggcctgt gaagatgcgg aagggcgggc
     2281 tgccggtgtt cagcaagccg ggtcggtgtt catcggccat tttgcgccgg aaacggcggg
     2341 agattacgcc agcggcacca accataccct tccgacaaat ggatatgcca gggcgtatag
     2401 cggagtaggc gtggatagct ttttaaagaa gatcagtttt caacagctct ccgaaagcgg
     2461 actttccggt atcagccaga cactgatcga gatggccagg gcggaaggtt tgcaggcgca
     2521 cgccgctgcc gtggaaatca gacttaaaca aatgaatact ccagctcatg cctgatatta
     2581 accgactgct tcgaaacaac attaaaaagc tggtgcctta ttcatcggcc agggatgagt
     2641 tcaccggaac cgccagcatt ttcctggatg ccaatgagaa tagtctcggg tctccgatcc
     2701 ccgaacaata taatcgctat cccgatcccc accaggtaaa gatcaaggaa atgctgagtg
     2761 caataaaagg cgttccggca aagaatatct tcctcggaaa cggcagtgac gaatgcatcg
     2821 atctgctgta ccgtgcgttt tgcaatccgg gccgggataa tgtgatcatt aacccgccaa
     2881 cctatgggat gtatgaagtg agtgcccgca tcaacgacgt ggaaatccgg aaggccgtcc
     2941 tgacggagga ctttcaaccc gatctggaga aaatggaaag cctcacggat gaaaatacga
     3001 aaatgatctg gctgtgttct ccaaacaatc caaccggcaa tgcctttcac tatcaaagca
     3061 ttgagatgat cctccataat tttgacggat tgctggtgat tgacgaagcc tatatcaatt
     3121 tttcccggca gcagagtttt atccgcgaac tgaccgaatt ccccaacctg gtggttctgc
     3181 aaacactcag caaggcctgg gggcttgccg ggctcagaat gggtatggct tttgcgagtg
     3241 agtccattgt tgaaatattg aatagaatca agccgcccta taatatcagt cagcctgccc
     3301 aggagctggt gctgaaagcg ctgaaggaga ccgggcgggt caatgaaagg atccgggaga
     3361 tcgtgcttat gcgggatttg ctggcggccg ggcttggcca ggttcccggc gtaaaaaaag
     3421 tttaccccag cgatgcaaac ttcttactgg ttaagatgga cagggcaaga gaggtctatg
     3481 aaaccctgtt aaagcagggt atcgttgtcc gggacaggag ccgggtggtg ctttgtgatg
     3541 actgcctacg gattaccgtt ggtaaaaaag aggaaaatga ggagctgctc
//
LOCUS       DASYCU010000304         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_938444, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000304 DASYCU010000000
VERSION     DASYCU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938444"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..715
                     /locus_tag="VGM24_06170"
     CDS             305..715
                     /locus_tag="VGM24_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4154989.1"
                     /translation="MKKLISTSYSDNAFNFAMLLQRVVVGLMLLIAHGLPKISNFNEM
                     SRTFFDPFQIGHRNTLLLSIFAEVFCSMLLVLGLFTRIAAFIIVLNLSCAVFLYHKGQ
                     PLRNVELGIIYLTSVFSILLLGPGRVSVDGMMGK"
     gene            742..1155
                     /locus_tag="VGM24_06175"
     CDS             742..1155
                     /locus_tag="VGM24_06175"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY4154990.1"
                     /translation="MFITETQIRVRYAETDQMNVVYYGNYAQYFEVARAESIRKLGFT
                     YREMEALGVIMPVVEMQTKYLRPAHYDDLLNIKTVLRELPVDHRISFEHEVFNQEKKL
                     LTVGKVVLYFVKMGTFEKSRMPQSLRSLLEPYFEV"
     gene            1206..2456
                     /locus_tag="VGM24_06180"
     CDS             1206..2456
                     /locus_tag="VGM24_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY4154991.1"
                     /translation="MTNASIITIGDELLIGQVTDTNSAWMAQELNKAGIWVKRRVAVG
                     DNGEEIRRTLDEESAVSDIILITGGLGPTADDITKPLLCDYFQTRMVVDEEALKNVKY
                     IFETVFKRPVIERNLKQAEVPESCTVIRNKRGTAPGMLFERNGKIFVSMPGVPHEMKG
                     MMTDEVIPFLMKKFRMPFIAHRTLLTAGVGESFLAEHIQEFETALPENIRLAYLPGYG
                     MVRLRLTGIGNDPAVIEPETDARFARLKELVSEWMVTDEDISLQEALGKLLKSRKQTL
                     AAAESCTGGYIAHLITSIPGSSAYFQGGLIPYQNRIKEKVLQVQENTLMTAGAVSEET
                     VREMITGTLNLFGSDYALAVSGIMGPDGGTETKPVGTVWIAVGNKQSTETRKLGFRFD
                     RLRNIELTSTNVLNLLRKFILSQP"
     gene            2524..>3312
                     /locus_tag="VGM24_06185"
     CDS             2524..>3312
                     /locus_tag="VGM24_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY4154992.1"
                     /translation="MAIVDVLMPKMGESIMEATILKWHKQPGDHINQDETLLDIATDK
                     VDSEVPSTAAGILEETLFQVNDVVAVGAVIARIKTEMTEAAGHSNQPPAPEKPEPEKV
                     YTPEDKPAGPDIDAGPAAPNGNGNRFYSPLVLNIAGSEGIPVAELEKIPGTGNEGRVT
                     KKDILQYVSGRKTENVSYAAQDDGGSAFSRPAESKAASDYSSASTANTTDAREYNPGG
                     NVEIREMDRMRKLIAEHMVRSKQTSPHVTSFSEADVTNLVLWREK"
BASE COUNT          886 a          786 c          880 g          760 t
ORIGIN      
        1 tgaaaatcgg gtaagccagg ctataatact gcttctgata atattcctgt gcctgtttgt
       61 atgccgactg cggatcattc agataccggg tctgctgagc atttgccgaa aaggaaaaaa
      121 taaggaggaa cggaaagaat aatcgtttca tccggaaata gttaatcacg cgcccaagtt
      181 tctgaatatt ttcttctatt accaaagatt gttccaaaca ggtgtggata actgaaagga
      241 ttaacatttc cttagaacaa gcggcaaaaa ccgcaaatcc actacttttg gataaatatt
      301 cgcgatgaaa aaactgatat ccaccagcta ttccgacaat gctttcaatt ttgccatgct
      361 gctgcagcgg gtggtggtgg gactgatgtt gctcattgcc cacgggcttc ccaagatttc
      421 aaattttaac gagatgtccc ggacattttt tgatccgttc cagatcggtc accggaatac
      481 gctgttgctt tcgatctttg cagaagtttt ttgttccatg ctgctggttc tcggattatt
      541 tacccggatt gccgcgttta tcattgtgct gaatttatcc tgtgccgttt ttctgtatca
      601 taaagggcaa cccctccgga atgtggagct gggcatcatt tatttaacca gcgtattttc
      661 cattttgctt ttgggcccgg gaagggtgag tgttgacggc atgatgggca aatagaaacc
      721 gggattaaat caaacgatca catgtttatt accgaaacgc aaatcagggt caggtatgcc
      781 gaaaccgatc agatgaatgt ggtctactac ggaaactatg cccagtattt tgaagtggcc
      841 cgggcagagt ccattcgcaa actcggcttc acctaccggg agatggaagc gctgggggtg
      901 atcatgcccg tggtggaaat gcaaaccaaa tatctgcgtc cggcacacta cgacgacctg
      961 ctcaatatta aaaccgtttt acgcgaactg ccggtggacc accgcatcag cttcgaacat
     1021 gaagtgttca accaggagaa gaaactgctc acggtcggaa aggtcgttct ctattttgta
     1081 aagatgggaa cctttgaaaa aagccggatg ccgcagtccc tgcgcagcct cctggagcct
     1141 tatttcgagg tatgaaaccg gcactcctgt ttcggcagga ccatttttaa agcattattc
     1201 caattatgac aaatgcatcc ataataacga tcggcgacga actgctgatc ggtcaggtaa
     1261 cagataccaa cagcgcctgg atggcgcagg aactcaataa ggccggcatc tgggtgaaac
     1321 gccgggtggc cgtcggcgat aacggggaag agatccggag aaccctggat gaagaatctg
     1381 ccgtttcgga tatcatcctg atcaccggcg ggttgggacc gacggcggac gatatcacca
     1441 agccgctttt gtgcgattat tttcagaccc ggatggtcgt ggatgaggag gccctgaaga
     1501 atgtgaaata tatattcgag accgttttca aacgccctgt catcgagcga aacctgaagc
     1561 aggctgaagt gccggagtcc tgcaccgtga tccggaacaa acgcggaacc gctccgggta
     1621 tgttgtttga acggaatgga aagatattcg tatccatgcc cggcgttccg catgagatga
     1681 agggaatgat gacggatgaa gtgatcccct tcctgatgaa aaaattcagg atgccattca
     1741 tcgcgcaccg caccctgctc acggctggag tgggtgaatc ctttctggcc gagcatatcc
     1801 aggaatttga aacggcctta ccggaaaata tccggctggc ctatcttccg ggttacggaa
     1861 tggtccgttt gcggttgacg ggaataggga atgatccggc cgtgattgaa ccggaaaccg
     1921 atgcccgctt tgcacggctg aaagaactgg tatccgaatg gatggtaacg gatgaagata
     1981 tttccctgca ggaagcattg ggaaaattat tgaaaagccg taaacagacc ctggctgcgg
     2041 cggagagttg caccggcggt tatatcgccc acctgatcac ttccattccc ggatccagcg
     2101 cctatttcca gggaggactc attccctatc agaaccggat caaggaaaaa gtgctgcagg
     2161 ttcaggagaa tacgctgatg actgccggcg cggtgagtga agaaaccgtt cgtgaaatga
     2221 tcacgggcac tttgaattta tttggcagcg attatgcgct ggccgtatcc ggcatcatgg
     2281 ggcccgacgg cggcaccgaa acaaaaccgg tgggtacggt ttggattgcc gttggaaata
     2341 aacagtcgac cgaaacccgc aaactcgggt tccggttcga ccgtctgaga aatatagaac
     2401 tcacttccac caatgtgctc aacttgttaa gaaaatttat tttgtcgcag ccctgattgg
     2461 tggctctccg cgacttaatt aataattaat atttactttt gaccgaacct aaagttcatc
     2521 atcatggcga ttgtagatgt gttaatgccg aaaatgggtg agagcattat ggaggccact
     2581 attctcaagt ggcacaaaca gcctggcgac cacattaacc aggatgaaac cttattggat
     2641 atcgcaacgg acaaggtgga cagcgaagtt ccctcgactg ccgcgggaat cctggaagaa
     2701 acgctgttcc aggtaaacga tgtggtggcg gttggagccg tgatcgcgag aataaaaaca
     2761 gagatgacgg aagcggccgg gcattcgaat caaccccccg cccccgaaaa gccggaaccg
     2821 gaaaaagttt atactccgga agataaaccg gccgggccgg atatcgatgc cggtcctgcc
     2881 gcccccaatg gcaacggcaa ccgcttctat tcccccctgg tattgaatat tgccggcagc
     2941 gaaggcatac cggttgccga actggagaag attccgggaa ccgggaatga gggcagggta
     3001 acaaaaaaag atatccttca atatgtttcg ggacgaaaaa cggagaacgt atcttacgcc
     3061 gcacaggatg acggcgggag cgccttttcc cggccggccg aaagcaaagc ggcttcagat
     3121 tattcctccg cgtccacggc aaatacaacg gatgcccggg aatacaatcc cggcggcaac
     3181 gtagagatcc gggaaatgga taggatgcgg aagctgattg cggaacacat ggtgcggagc
     3241 aaacagacca gcccgcacgt aacgagcttc tccgaagcgg acgtgaccaa cctggtgtta
     3301 tggcgtgaaa aa
//
LOCUS       DASYCU010000305         6241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_791300, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000305 DASYCU010000000
VERSION     DASYCU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791300"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VGM24_06190"
     CDS             <1..700
                     /locus_tag="VGM24_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4154993.1"
                     /translation="SRPGSRVSGNEFDIYRPRVDAAPATRGGAATRPAPARAQNFRDL
                     RPQTQNNSTRPVTQPAAQNPAINNNPRPAAPPANNPRPAAPPANNPRDVTQPPAQNPA
                     FNNNNNPRPVPQPATVNPNPATTGRPADSRYNQRPGNAQPIQQRTLPQPSPANRRQMP
                     AQPPANAPANRAQPVQQRTLPQPSPANRRQMPAQPPANRPQPVRQGTPAPQAHRPVRP
                     EEKKTDKPVTRDAN"
     gene            1207..2766
                     /gene="dnaB"
                     /locus_tag="VGM24_06195"
     CDS             1207..2766
                     /gene="dnaB"
                     /locus_tag="VGM24_06195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY4154994.1"
                     /translation="MELTNLNKDRKLRRKSPLDVSTLMYGKVPPQARELEEAVLGAVM
                     LEKAAFDTVIEILKPECFYVESHQHIYRAMQSLAQKSQPIDMLTVVEELKFREELEIC
                     GGPYYITRLTNSVVSAANIDAHSRIILQKFIQRELIRISGEIIGDAYEDSTDVFDLLD
                     DAEGKLFEITNNHLRKNFDSIDTVLVKTIQRIEDLRNKNEDISGVPSGFASLDRITYG
                     WQPTDLIVLAARPSVGKTAFALNLARHAALHPTKSTPVAVFSLEMSASQLVQRILSAE
                     SEIWLEKIARGKLEEHEMKQLYAKGIQRLAQAPIFIDDSAALNIFELRAKCRRLKNKH
                     NVGLIIIDYLQLMSGTGDNRNGNREQEISRISRDLKGLAKELQVPIIALSQLSREVEK
                     RKEGNKMPQLSDLRESGAIEQDADMVMFLYRPEYYDITANEFGESNKGETHVRIAKHR
                     NGSLETVKLKALLHIQKFIEDDGAGGFDQLPPGGNWKPVPGDQGGPKLYIQAGSKMND
                     IPFNDEEEAPF"
     gene            2799..3506
                     /locus_tag="VGM24_06200"
     CDS             2799..3506
                     /locus_tag="VGM24_06200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY4154995.1"
                     /translation="MPAVHFYLASLSHMAEEFPLNEPASRHIVQVLRMQAGEDLVLTD
                     GRGLEAVATILDAHKKHCQVRISERKFRKPDARRVTVAISLLKNGSRFEWFLEKATEL
                     GISEIIPLKCSRTEKQQFRKERMQSIMESALIQSQQSWMPLLQEPAAFPEFIENAVSD
                     QKFIAYCGPAEKRELREMIDISLSSHLILIGPEGDFTGEEIRLAFQSRYIAVSLGEHR
                     LRSETAAVAAAALLKLV"
     gene            complement(3510..4571)
                     /locus_tag="VGM24_06205"
     CDS             complement(3510..4571)
                     /locus_tag="VGM24_06205"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY4154996.1"
                     /translation="MYKQLRNLLFLLPPEAAHHLSMGLFGLFSGNAITRPLLRRAFSV
                     NDPALEKDFTGLHFKNAVGLAAGFDKNARYLQSLETLGFGFVEIGTVTPEPQNGNPKP
                     RLFRVKKDQALINRMGFNNDGADRIAKRLQRWKEKKPDSGNGRLIIGGNIGKNKVTPN
                     DDAWKDYETCFIKLFPWVDYFVVNVSSPNTPGLRELQEKESLRKILGTLQQCEPQRKT
                     PKPILLKLAPDLPLEQLGEVLDLLMEIGLDGLVISNTSTSREGLVSSTGFLDRIGQGG
                     LSGKPLQKKSTSLIRYISARTREQLPVIASGGIFNPADALEKLNSGAGLVQVWTGFIY
                     EGPAIAKKILKALKKTLSH"
     gene            4688..5866
                     /locus_tag="VGM24_06210"
     CDS             4688..5866
                     /locus_tag="VGM24_06210"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY4154997.1"
                     /translation="MQEAYIVAGFRTAVGKSKRGGFRFYRPDDLAIEVIKGLLASVPQ
                     LDPKQVDDVIVGNAVPEAEQGLQVGRIIAARAVGIHAAGVTVNRYCASGLETIAIATA
                     KIRTGMAECIIAGGTESMSMVPTAGWKPVPSYSIASDDPDFYLSMGLTAEAVAKEYKV
                     SREDQDLFSYQSHQKALNAIKNGYFKPGILPVKVEETFLDKQGKKQKRDFVVDTDEGP
                     RQDTSPEALAKLKPVFAAGGSVTAGNSSQTSDGAAFVIVMSENLVKQLGLKPVARLVA
                     CASAGVHPRIMGVGPVEAVPKVLKQAGMSLQQIDLIELNEAFASQSLAVIRELGLNPE
                     IVNINGGAIALGHPLGCTGCKLTIQITHEMKRLNKKYGIVTACVGGGQGIAGIIENIH
                     "
BASE COUNT         1572 a         1632 c         1632 g         1405 t
ORIGIN      
        1 cagccgcccg ggaagccggg tatccggaaa tgaatttgac atctaccggc cccgggtaga
       61 cgcagctccc gccacccgcg gaggtgcagc gaccaggccg gccccggcaa gggcgcaaaa
      121 cttccgggac ctgcgacccc agacccagaa taatagcacc cgcccggtaa cgcagccggc
      181 tgcacagaac ccggctatca ataataatcc gagacctgcc gcgccacctg caaataaccc
      241 gagacccgct gcgccgcctg caaataatcc gcgtgatgta acgcagcctc ctgcacagaa
      301 tccggcattt aacaacaata acaatccccg tcctgtaccg caacccgcaa cggtaaaccc
      361 caatccggca acaacggggc gacccgctga ttcccggtac aaccaacgac cgggaaatgc
      421 acaaccgata cagcaacgga cccttcccca accttctcct gcaaaccgca ggcagatgcc
      481 ggcgcaaccg cctgcaaacg ctccggctaa ccgggcacaa ccggtacagc aacggaccct
      541 tccacagcct tctccggcaa accgcagaca gatgccggcg caaccgcccg ccaaccgtcc
      601 gcagcccgtg cggcagggca cccctgcacc gcaagcgcac aggccggtga gaccagagga
      661 aaaaaagaca gataagccgg taacaagaga tgcgaattaa ttccggaagt ttaataatat
      721 gagttcagcg acggtcccgg atcaaccggg accgttgttt taggatcctg tattactatt
      781 ttgcaaaggg ccgggcccgg atatttaatc cgatttaaaa agaaatattc atccccgtga
      841 tttcagctga ttccggtttc tttattttct gccggggaac aaaaaagaga ttcttgttct
      901 gcaaaatttt acaggagaaa tctttctgca cagccggtgt gcatttataa accggattag
      961 cgaaaaactt ttttaaattg tggatagttg gaaacaattt gtgtataacc ccttgtttta
     1021 tgttcgtaac cttgtgcatt caggctggac aaatttaaaa aatgaaaact ttttttaaag
     1081 gaattaatgg ggtttatatt cgtccccctg atatcatttg gtaacacttg catcatttta
     1141 atttaatcat ttcctaacac agccaactcc cacttctgaa agagtggaag caaaccaagc
     1201 aaataaatgg agctcacgaa tttgaataag gaccggaaac tgagacgcaa gtctccgttg
     1261 gatgtcagca ccctgatgta tggcaaagta ccgccgcagg cccgtgagct ggaggaagcg
     1321 gtgctggggg ccgtcatgct cgaaaaggca gcatttgaca ccgttattga aattctgaaa
     1381 ccggagtgct tttatgtgga aagccaccag cacatttacc gcgccatgca atccctggcc
     1441 cagaaaagcc agccgatcga catgcttacg gtggtggaag aattgaaatt ccgggaagaa
     1501 ctcgagatct gcggagggcc ttattatatc acccggctga ccaactccgt ggtgtctgcc
     1561 gccaatattg acgcgcattc ccggattatt cttcaaaaat tcattcagcg cgagctgatc
     1621 cggatttccg gggagatcat tggagacgca tacgaagaca gcacggatgt ttttgacctg
     1681 ctggatgatg cggaaggcaa acttttcgaa atcaccaata accacctgcg caagaatttt
     1741 gacagtatcg atacggtgtt ggttaaaacg atacagcgga ttgaagacct ccggaataag
     1801 aatgaagata tttcgggtgt cccctccggt tttgcatcgc tcgaccggat cacctacggc
     1861 tggcagccga cggatctgat cgttctggca gcccgcccct ccgtgggtaa gaccgctttt
     1921 gcgctgaacc tggccaggca cgcggccctt caccctacga aatccacgcc cgtggccgtt
     1981 ttcagcctgg aaatgagcgc aagccagctg gtgcagcgca tcctttccgc cgaaagtgag
     2041 atctggctgg aaaagattgc ccggggaaaa ctggaagaac atgaaatgaa gcaattgtat
     2101 gccaagggca tacagcggct ggcccaggcg cccattttca ttgatgattc cgctgccctc
     2161 aatattttcg agcttcgcgc caaatgccgc cggctgaaga ataaacacaa tgtcggtttg
     2221 atcatcatcg attatttgca attgatgagc ggtaccgggg acaaccggaa cgggaaccgg
     2281 gaacaggaaa tcagccgcat ttcacgggat ctgaaaggtc ttgccaaaga actacaggtg
     2341 cccattatcg cgctttccca gttaagccgg gaagtggaaa aaagaaagga aggaaacaaa
     2401 atgccgcagc tgagcgatct gcgggaatcg ggcgccattg aacaggatgc cgacatggtc
     2461 atgttcctct accggcccga atactacgat attacggcaa atgaattcgg tgaaagcaat
     2521 aagggggaaa cccatgtgcg tatcgccaaa caccggaatg gttcgctgga aaccgtaaag
     2581 ctcaaggccc tgctgcatat tcagaaattc atcgaggatg acggcgccgg aggattcgac
     2641 cagctccctc ccggaggcaa ctggaaaccg gtgccgggag atcagggggg acctaaatta
     2701 tacattcagg caggaagtaa gatgaatgat attcccttca acgatgaaga ggaagctccg
     2761 ttctgaccgg ttatcctcct gcatttacca tcaacgatat gccggctgtc catttttatt
     2821 tagcttccct gagccatatg gccgaggaat tccctttgaa cgaaccggca tcccgtcata
     2881 tcgtgcaggt gctccgcatg caagcgggag aagatctggt cctcacagac gggcggggtc
     2941 tggaagcggt ggccaccatc ctggatgccc ataaaaaaca ttgccaggtg cggatctccg
     3001 aaagaaaatt ccggaagccc gatgcccgcc gggtgacagt cgccatttcc cttttaaaga
     3061 atggcagccg cttcgaatgg ttcctcgaga aagcaaccga actgggtatt tcggagatca
     3121 tcccgctaaa atgcagccgg acggaaaaac agcagttccg taaggaaaga atgcaatcca
     3181 ttatggaaag cgcactgatc cagtcacagc aatcctggat gcccctgttg caggagcccg
     3241 ccgcatttcc cgagttcatt gaaaatgccg tttccgacca gaaatttatt gcttattgcg
     3301 gaccggcgga aaagcgggaa ctgcgtgaaa tgatcgatat cagcctgtcg tcccatctga
     3361 tcctgattgg tcccgaaggg gatttcacgg gtgaagagat ccggctggca tttcagtcca
     3421 ggtatattgc ggtcagcctc ggggagcacc ggctccggtc ggagaccgct gccgttgcgg
     3481 cggcggcctt attgaagctg gtttgacggc taatgagaaa gggtcttctt cagggccttc
     3541 agtatttttt ttgcgatggc gggaccttcg taaataaacc cggtccacac ctgcaccagg
     3601 ccggctccgc tgtttaattt ttccagggca tccgccggat taaaaatacc acccgaggct
     3661 atgacgggca gctgctcacg agttcttgcg ctgatatacc ggatcaggct ggttgatttt
     3721 ttttgcaggg gttttccgct aaggccgcct tgcccgatcc ggtccaggaa gccggtggaa
     3781 gagaccagcc cctccctgga agtgctggta ttgctgataa cgagcccgtc cagcccgatc
     3841 tccatcagca ggtcaaggac ttcgcccagc tgctccaaag gcaggtcagg cgctaatttc
     3901 agcaggatgg gtttgggtgt ttttcgctgg ggttcgcatt gttgcagggt gccgaggatc
     3961 tttctcaggg attctttctc ctggagttcc cggaggccgg gggtattcgg agaactgaca
     4021 ttcaccacga aataatccac ccagggaaaa agtttaataa aacaggtttc gtagtctttc
     4081 caggcatcgt cgttgggcgt gactttattc ttaccaatat tgccgccgat gatcagccga
     4141 ccgtttccgg aatccggctt tttttccttc caccgctgca gccgttttgc gatgcggtct
     4201 gctccatcgt tgttaaaacc catccggttg atgagcgctt ggtctttttt tacccggaac
     4261 agccggggct tcgggtttcc attttgcggt tccggagtta cggtcccgat ttccacaaag
     4321 ccgaacccca gggtttcgag gctttgcaga taccgtgcat ttttgtcgaa ccccgctgcc
     4381 agtcccacgg catttttaaa atgcaatccg gtgaaatcct tctccagggc aggatcgttt
     4441 accgagaaag cccgccggag caaaggccgg gtaatggcat ttccggaaaa caggccgaac
     4501 aaacccatgg acaaatgatg tgcggcctcg ggcggtaaaa gaaataataa attacgcagc
     4561 tgtttgtaca tgaacggttc aggtgacgag taaaaaatga aagtaaagat ataccacttt
     4621 aacagttgca tttggcagtg agccttcgaa aaatttatct ttggtattcc atcattcaat
     4681 tcattttatg caggaagctt atatcgttgc cggattccgt acggcggtgg gtaaatccaa
     4741 acggggcgga ttccggtttt accggcccga cgacctggcc atcgaagtga tcaaagggtt
     4801 actggcatcg gttccgcagc tggacccgaa gcaggtggat gacgtgatcg taggcaatgc
     4861 cgtgcccgaa gcggaacagg gattgcaggt agggcgcatt attgccgccc gggcggtagg
     4921 cattcatgcc gccggggtaa cggttaaccg gtattgcgcc agcggactgg aaacgattgc
     4981 cattgcaacg gccaagatcc gtaccggaat ggcagaatgc atcattgccg gcggcacgga
     5041 aagtatgagt atggttccta cagcaggctg gaaaccggtg ccttcctatt ccattgcatc
     5101 ggacgatccg gatttctacc tcagtatggg gctcacggca gaagccgtcg caaaagaata
     5161 taaggtgagc cgggaagacc aggatctgtt ttcataccag tctcatcaga aagccctgaa
     5221 tgcaataaag aacgggtatt ttaaaccggg cattctgccg gtgaaagtgg aagaaacttt
     5281 cctcgataag cagggaaaaa aacaaaaacg ggatttcgta gtggatacgg atgaaggtcc
     5341 gcgccaggat acttcacccg aagcgttggc gaaactaaag ccggtctttg ctgccggcgg
     5401 gtcggtcact gccggtaatt cttcccaaac cagcgatggg gcggcttttg ttatcgtaat
     5461 gagcgaaaat ctggtgaagc agctgggcct gaaaccggtc gcccggctcg tggcttgtgc
     5521 gagtgcaggc gttcatcccc gaattatggg cgtggggccc gtggaagccg ttccaaaagt
     5581 attgaaacag gccgggatga gcctgcagca gatcgacctg atcgaattaa acgaagcctt
     5641 tgcgagccag tcgctggccg taatccggga actcggattg aatcctgaaa tcgtcaatat
     5701 taacggcggc gctatcgcgc tcggtcatcc gctgggttgt acgggttgta agctcacgat
     5761 ccagatcacc catgaaatga aaaggttgaa taaaaaatac ggcatcgtaa ccgcctgcgt
     5821 cggtggaggt cagggaatag ccggtataat cgaaaatatc cattaagatt ttggtaaaga
     5881 attgttattg cttccgtttg aatggcggtc ttcctaattt tgcggactcc ctaatttgaa
     5941 acaacatcag atcatacgaa gattttccgc agcttttttt ctgctgttgt tcactttttg
     6001 cgttacgcca aagagatttc ttcacgactt actggccaat cataaggaca ttgaacgaac
     6061 cgcgaaccag ccgttccagg aaatcgctgc ctcgggcttt cattgtcatt gcgaccggct
     6121 cgtggtcatg gcaccctttc tcccggagat ccgcccgcag gaaacgccgc tgatctcctt
     6181 cactgcgccg gtatttaccg agtcgctcac cgcctttgtt tttactttcc ttacccacac
     6241 c
//
LOCUS       DASYCU010000306         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1664684, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000306 DASYCU010000000
VERSION     DASYCU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664684"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VGM24_06215"
     CDS             <1..728
                     /locus_tag="VGM24_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223114.1"
                     /note="hemoprotein; NADPH dependent; with the alpha
                     subunit (a flavoprotein) catalyzes the reduction of
                     sulfite to sulfide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase subunit beta"
                     /protein_id="HEY4154998.1"
                     /translation="EEIARRTGFTLEKERPFEFVDRKDYYGWYKNAEGLWYYTPFVEN
                     GRIVDSEEAALKTALYEIAQTGKLNFRFTTNQNVILSDITQKDKPVIEKILNKYKINE
                     YTDSISAIRRNSMACVALNTCPLAFAEGQRYLPSLISKIEPLLNKYGLEKEEIIIRMT
                     GCPNGCARSYIAEIGFVGTSLGKYNLQLGGDHQGMRLNKVYRESLDEAGILNELDSLF
                     LLFSKEREQKESFGDFAMRKQLV"
     gene            960..2519
                     /locus_tag="VGM24_06220"
     CDS             960..2519
                     /locus_tag="VGM24_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEY4154999.1"
                     /translation="MSEKNESGNRNQAVEEAQAGRNHLFPVFLKLEELGVLLVGGGKV
                     GMEKLSAILANAPATLVTVVATEISEPVKQLAGSNSSIRLIERPFEPDDLEDKDIVVV
                     AVNDKSVSNYVRVLAKEKKLLVNVADTPDLCDFYLGSIVDKGNLKIAISTNGKSPTIA
                     KRMKEQLNELIPDEIEGVLQNMHTIRQDLKVDFAEKVKQLDDLTKVLSAKQAKLDQIS
                     KPGGRKWQGIVKWCLFAFFFMLFGHGVLSFIPFHEISEGIQKIPEVMDVPSFLMMLFT
                     GFLAQMVDGSLGMGYGTISTTFLLANGVSPAIVSSRVHSARVFSSGVSGYSHHRFGNI
                     NKKLFRALVIPGVIGAILGATLAYFGQKYATWVRVPLSLYTLYLGYYIIGKAFRKKNK
                     QYKVKNAGWLASVGGFMDAFAGGGWGSLVTGTLISKRRNPRYVIGSVCLAEFFVVFAS
                     SITFFILLKTIFIVDVAGLIVGGLIAAPIAARLVGKIPMKYMFMGVGSLVIITSILTL
                     IKGISNALISF"
BASE COUNT          696 a          569 c          662 g          628 t
ORIGIN      
        1 aggaagaaat tgcgcggcgc accggattca ccctggaaaa agaacggcct tttgaattcg
       61 tggaccgcaa agattattac ggatggtata aaaatgcgga agggctttgg tattatacgc
      121 cgttcgtcga gaacgggcgg atcgtggaca gcgaagaagc ggccctgaag acggcgctgt
      181 atgaaatcgc gcaaacgggc aagctgaatt tccggttcac caccaaccag aatgtgatcc
      241 tttccgacat tacgcaaaaa gataagccgg tcattgaaaa aatactgaac aagtataaga
      301 tcaatgaata taccgattcg atttccgcca tccgcaggaa ttcgatggcc tgcgtggccc
      361 tgaatacctg tcccctggcc tttgcggaag gacagcgtta cctgccctcc ctgatcagta
      421 agatagagcc gctgctgaat aagtacggac tggaaaaaga agagatcatc atccggatga
      481 cgggctgccc caatggctgt gcccggtcct atatcgccga aattggtttt gtcggtactt
      541 ccctgggaaa atataacctg cagctgggcg gcgatcacca gggcatgcgg ctcaataaag
      601 tttaccggga aagcctggat gaggcaggta ttttaaacga actggattct ctttttctgc
      661 ttttttcaaa ggaaagggaa caaaaagaat ctttcggaga ttttgcgatg cgaaaacagc
      721 tcgtttgatc cattgcgaat attctataaa aacaaccccg gccgatgttg aaggatttta
      781 ataaaagatc aaaataaaaa tttaatagtc tacaaattaa gtagggaata ccttattttt
      841 gggcctgccc ggtttgagaa gcgggaacag agtatgaaaa gagtatgaac agagtccggc
      901 cagagtatga ccagaacgag agcagaacgg gaaccgggtg ctgattaatg atgtcattta
      961 tgagtgaaaa aaacgaatcg gggaaccgga atcaggccgt ggaagaggca caggcgggca
     1021 ggaaccacct gtttcccgtg ttcctgaaac tggaggaact gggggttttg ctggtcggtg
     1081 gcgggaaagt gggcatggaa aaactgtcgg cgattttagc caatgcaccg gcgaccctgg
     1141 tcaccgtggt ggcgacggaa atctcggaac ccgttaaaca gcttgccgga tcgaattcct
     1201 ccatccgcct gatcgaaagg ccctttgaac cggatgatct cgaggataag gatatcgtgg
     1261 tggtggcggt aaatgataaa tcggtcagta attatgtgcg ggtactggcc aaggaaaaga
     1321 aactcctcgt caatgtggcg gataccccgg atctgtgtga tttctacctg ggatccatcg
     1381 tggataaggg aaacctcaag atcgccattt cgaccaacgg caaatcccct accatcgcca
     1441 agcggatgaa ggaacagtta aacgagctga tccccgatga aattgaaggc gtattgcaaa
     1501 acatgcatac catccggcag gacctgaaag tcgattttgc ggaaaaagta aaacagctcg
     1561 acgacctgac caaggtgctc agcgccaagc aggccaaact ggaccagatc agcaaacccg
     1621 gcgggagaaa atggcaaggc attgtgaaat ggtgcctgtt tgcttttttc tttatgctat
     1681 tcggtcatgg cgtactctcc tttatcccgt tccatgaaat atcggaagga attcaaaaga
     1741 ttccggaagt tatggatgtc ccttcctttc tcatgatgct gtttaccggg ttcctggctc
     1801 agatggtaga cggttccctg ggtatgggat acggcaccat atccaccact ttcctgctgg
     1861 ccaacggcgt aagtccggcc atcgtcagca gtcgcgtgca ttctgcccgg gttttttcca
     1921 gcggcgtatc cggatacagc catcaccggt ttggaaatat caataaaaaa ttattccggg
     1981 ccctggtaat acctggggtg atcggtgcaa tactcggagc aaccctggcg tatttcggac
     2041 aaaaatatgc cacctgggtg cgcgtgcccc tttctctcta cactttatac ctgggttact
     2101 atattattgg caaggcattc agaaaaaaaa ataaacaata caaggtaaaa aatgccggct
     2161 ggctggcttc cgtcggtgga tttatggatg cttttgccgg cggaggctgg ggttccctgg
     2221 ttaccggaac actcatttca aaaagaagaa acccacgtta tgtgattggc tcggtttgtc
     2281 ttgctgaatt cttcgtggtg ttcgccagtt ccattacttt tttcattctg ttaaaaacga
     2341 tattcattgt ggacgttgca ggcctgattg tcggaggcct gattgcggca ccgattgcgg
     2401 caaggcttgt cggaaaaata cccatgaaat atatgttcat gggcgtcggt tcactggtta
     2461 ttatcaccag catactcacc ctgatcaagg ggatttcaaa cgctttaata agtttttaat
     2521 ttgaacagtc aaatctggtt aatgattttt tatac
//
LOCUS       DASYCU010000307         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4124965, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000307 DASYCU010000000
VERSION     DASYCU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4124965"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VGM24_06225"
     CDS             <1..1016
                     /locus_tag="VGM24_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155000.1"
                     /translation="NTVMPQGLFWIRAAIPKNSRSVSETTAIFTQAISTTFINSDPND
                     KLRLSQPLPGGSLSKLEIADASVKTVSQPYDSFGGELPEIQQQFYVRVSETLRHKGRA
                     IQAFDYERLALEKFPQLFKVKCINHSFALNAHEYKNDFPYAGGYVILAVIPDLNKLKA
                     GNSFEPKVPVSMIEAVDDYIRKKTSPFVRFRAMNPRYEKINFCLRIRLLPGRDENYYK
                     EQVREDIREFLAPWAVGEFSKLSFGQCVYRSDIIRFLETRDYMDFISDLRMAGDGETP
                     DGTRPKICPDTPRSILIAGNIEVCIDDPDCPQWGPYTACPEDQKPITTCENIPEKISD
                     YCK"
     gene            1037..>2445
                     /locus_tag="VGM24_06230"
     CDS             1037..>2445
                     /locus_tag="VGM24_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155001.1"
                     /translation="MADSTNDKTINRINTGFPDYLDFDTLRSSAIAYLGNLTGKIWTD
                     YNVHDPGITTLEVLIYALLDLGYRTNLPVDDLLTRDPSDSTTDNNFFPASRILGNNPL
                     IITDYRKLLVDISGVKNAWLEPDDHTSVDFCGEKQSPPPGAVILEEVYIPPSDPCQCS
                     FLNGLYHVYIQLDDDIDPADKHQVGKITRAVRDSLLAHRNLCEDFMDIQILCKLDLGI
                     CAEIDLATGADAETVYISIVEALQEYFSPAPKFYSLQQLLDRGKPIDEIFAGRPYNLT
                     QSHGFVDTDEFEQLTLRKELHLSDVYHLLFAIPGINRIRNLGWIKCCKDKQSVPDWKL
                     VLPENYIPSFSTACSGFVFTRNGLPEKVDTKKFDAYFEIKFSGVKKAVYTEPSAFLDP
                     VLPKGVYRNDLADYYSIQNEFPRVYGISEGGLSSEEPDKRKAQALQLQGFLLFFDQLL
                     ANYLTQLKNIRSLFALTSSA"
BASE COUNT          626 a          672 c          596 g          551 t
ORIGIN      
        1 ataataccgt catgccccaa ggcctcttct ggatcagggc ggccattccg aagaacagcc
       61 gttcggtgag cgaaacgacc gccatattca cccaggccat cagcaccacg ttcatcaaca
      121 gtgatccgaa tgacaaatta aggctgtcgc aacccctgcc cggcgggagc ctttcaaaac
      181 tggagattgc cgatgcaagt gtgaaaacgg tcagccagcc ttacgattct ttcggaggag
      241 aattgccgga aatccagcag cagttctacg tgcgcgtgag cgaaacgctc cggcataagg
      301 gacgggcgat acaggcattc gactacgaac ggctggccct ggaaaagttc ccgcagttgt
      361 tcaaggtaaa atgcatcaac cacagttttg cactgaacgc ccacgaatac aaaaatgatt
      421 ttccttatgc cggcggctat gtgatcctgg ccgttatccc ggacctgaac aaactgaaag
      481 cgggaaattc cttcgagccc aaagtgcccg taagcatgat cgaagccgtc gacgactata
      541 tccggaaaaa gacttccccg tttgttcgct tccgggcaat gaacccccgc tatgaaaaaa
      601 taaatttctg tctgcgtatc cgtctgcttc cgggccggga tgagaattat tataaggaac
      661 aggtccggga agatatccgg gaattcctgg cgccctgggc cgtcggcgaa ttcagcaagc
      721 tcagcttcgg gcagtgcgtt taccgctcgg atattatccg cttcctcgag acccgcgact
      781 atatggattt tatcagtgat ctgagaatgg cgggcgatgg cgaaacgccg gacggcaccc
      841 ggccgaaaat atgcccggac actccgcgct ctatcctgat cgcggggaat attgaagtat
      901 gtatcgacga tcccgactgc ccgcaatggg gtccttatac ggcctgcccg gaagatcaga
      961 aacccattac aacctgtgaa aatattcctg aaaaaattag cgactactgt aaataaaaac
     1021 agaccgatga taagccatgg cggacagcac aaacgataag accatcaaca gaatcaatac
     1081 cggttttccg gattatctgg attttgatac ccttcgcagc agcgccattg cgtatctcgg
     1141 aaatctcacg ggtaagatct ggaccgatta caacgtccac gatcccggca tcactaccct
     1201 ggaggtgctg atctatgcct tgctcgacct gggttaccgc actaacctgc cggtggatga
     1261 cctgcttacc cgtgaccctt cagacagcac tacggataat aatttctttc ccgcatcgcg
     1321 tattctcggc aataatccgc tcatcattac cgattaccga aaactcctgg tagatatcag
     1381 cggcgtgaaa aatgcctggc tggaaccgga cgaccataca agcgtcgatt tctgtgggga
     1441 gaaacagagc ccacctccgg gtgccgtgat cctggaagaa gtctacatac cacccagcga
     1501 tccctgccag tgcagttttc ttaacgggct atatcacgtg tacatccagc tggacgatga
     1561 tattgatccg gcggacaaac accaggtggg aaagatcacg cgggccgtgc gcgactccct
     1621 cctggcgcac cgtaatcttt gcgaggattt tatggatatc cagatcctgt gcaaactcga
     1681 tctcggcatt tgtgcggaga ttgatctggc aaccggcgct gacgccgaaa cggtctacat
     1741 cagcatcgtc gaggccctgc aggaatattt ttcgccggcg ccaaaatttt attccctcca
     1801 gcaactcctg gatcgcggga agcccatcga tgaaatattt gccggccgcc cctataacct
     1861 tacgcaaagt cacggcttcg tggataccga tgaattcgag cagctaacct tgagaaagga
     1921 attgcatctc tcggatgtct atcatttact ttttgccatt cccggaataa accggatccg
     1981 gaacctgggc tggatcaaat gctgcaagga caagcaaagt gtcccggact ggaaactcgt
     2041 gctgccggag aactatatcc cttctttctc gactgcctgt tccggatttg ttttcacccg
     2101 caatgggctg ccggaaaaag tggatacaaa aaaattcgac gcttattttg aaattaaatt
     2161 ttccggcgtt aagaaagccg tctatacgga accttccgct ttcctggatc cggtcctgcc
     2221 aaaaggcgta taccggaacg acctggcaga ctattattcc attcaaaatg aatttccacg
     2281 ggtttacggg atcagcgaag gcggcctcag cagcgaggag ccggataaaa gaaaagcaca
     2341 ggccttgcag ttgcagggct tcctgttgtt cttcgaccag ctgctcgcca attacctgac
     2401 gcagttaaaa aatatccgct cactcttcgc gctgacctct tccgc
//
LOCUS       DASYCU010000308         7104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_647552, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000308 DASYCU010000000
VERSION     DASYCU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647552"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2061
                     /locus_tag="VGM24_06235"
     CDS             <1..2061
                     /locus_tag="VGM24_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4155002.1"
                     /translation="GYQFQEAMFPSTNNKKYNLALNLDAQATATTKISFNINGRSQNS
                     QYPAIAPGRVFELLQYVNTRRGALFFTNGKPGDKIPATIYGSGYEKINTLALYSQLSI
                     EQDLPFIPGLKAKGTVAYDPSNGLDKLWRIPTHITSIVDTSQHPYVFKDAIFDQAQAS
                     LNETNRQSYQLTYQASLNYVKSFRNNNITALALFEAKSNDSLYLAASRRNYNVNIDEI
                     DLGSSSQADMTTNGSSSATRQLGLLYRVTYNYSQKYFFEASGRYDGSYYFAPGKQFGF
                     FPAFSAGWRLSQENFLKDISWIDNLKIRGSYGEVGALAGAPFQYLSTYSVYGPAYVLG
                     GQAVQAASERAESNPNITWERAKKTDIGLDLSLWNGLLTLEADYFYEKRSNMLVSPNV
                     IVPSEYGIGLSQVNGGIMENKGIDFSIGSAYSISKDFQISLQGNFTYARNTLLKVFET
                     SATYDNPNRRITGKPLGTQFGFHAVGYFQEADFNPDGTLKSGIAVQPWGAVNPGDLRY
                     EDINHDGQINNDDLTKIGNSPTPRIIYGISPSVRYKGLSLDLLFQGAADANLYMDRQM
                     QWPFYNSMNAYVANFNSWTPDNPNAKYPRITNAPTTNNSQQSSWWMTNVSYLRLKSAT
                     LAYVIPSFIISRIKMQSVRVFLSGQNILTWTKLINYDPENAGAYTNLYPQQKVISAGL
                     NISF"
     gene            2084..3736
                     /locus_tag="VGM24_06240"
     CDS             2084..3736
                     /locus_tag="VGM24_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155003.1"
                     /translation="MSYIFNNARIAVLILAITGLVSCSKYLDVTPKDQVTNSALWSST
                     GNADLFLNNIYSSLPDPYNKLESEDTWTDNAMAGYNYYDSRAIYARSVYTPVNAPNYW
                     VNYNYVRKCNVFIQNVSASTTLPDAWKKQRLAEARYLRAYYYQLLWTHYGGVPIITDV
                     LNENTQGDSIFRVRNTDEETFKFIDDECAAIADDLPAKSDAGRATKGAVLTLKGWCEL
                     FEASALKNPENDPAKWALAAATNKQVMDLGVYSLFPDLESLFFEGNNNNSETIFARTY
                     LGGSATLGGSKEGLWGLRNVGGAQVGYGGVNPTQELVDDYEMANGLPITDPASGYDPQ
                     APYVNREKRFYQSIIYDGCTWLGYVILTRRGVGSKNEIDLTNGSGGGPNTGYALRKTL
                     DPKYAVSGSNHLNSANYNIFRYAEVLLSYAEAQNEAVGPDASVQDAVNQVRARSDLPA
                     LPTDLTQDEMRTAIRRERRVELSFEEKRWYDLIRWKTAEINLNTPSLHCMVIEKVNDV
                     WTYTVEPAADGSRIFYANKNYVLPIPQDAIDKNTKLVQNPNY"
     gene            3856..5532
                     /locus_tag="VGM24_06245"
     CDS             3856..5532
                     /locus_tag="VGM24_06245"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY4155004.1"
                     /translation="MNNRRDFLKQVSALGALSGLPNTLSAQSESGPDLTWYASGNGGV
                     VGSGPGPSAQAGIEMLNKGGNAADGAAAALFNLMVCDYGNFCIGGEVPFMYYNVKDGK
                     INVFNGMGGAPRDPKAIEWYYRHGIPNKRGIKASTTPSAVSTCLKALEVKGTMSFEQV
                     IAPTLSLLDAGGKKWYANLATTFRKLVETERSTGGSREKKIRAARDRFYKGDIADELN
                     DYYIRSEGFLRKSDLEAHETLIEDPVSINYRGYDVYKCNTWTQGPVLLQTLRLLENFD
                     LKSMGFLSANYIHTLAEAMKLAYADRDKYYGDPAFVKVPLKELLSDEYTAIRWPLIDK
                     NYASQAIRPGDPYKMQADAGAGEYWPGESGTTTCVVVDKWGNVAAATPSANPEYAICE
                     SLGIAHNTRLSSLNTQKGHPNSLEPGKRPRITLTPTIVLKDGKPIIAQSINGGDVQDQ
                     VSLELFLDFAEFGMMPKEAVSAPRFRTYHFENSFNPASYRASRILRLGGLTINSFNES
                     ILETLLTRGHSVNGVKGTMGIPVMAYIDQKTGISYVAGEPRIRFCAARNL"
     gene            5557..>7104
                     /locus_tag="VGM24_06250"
     CDS             5557..>7104
                     /locus_tag="VGM24_06250"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY4155005.1"
                     /translation="MSNRRKFIKQISALGVLGSLPDMLGAQAAPGQELTWYASGSGGA
                     VAAGPKVSAQAGIDILEKGGNAIDGAVAVIFNLAVSNYGLFCMGGESPFMCYTAKDAK
                     VHVFNGMGGAPKDPAAIEWYYKHGIPDLFGPEKGGIKAATTPSAVSTCLKALETKGTF
                     SFEQVIASTLSLLDAGGKSWYPNLAVTLRKMVETERNTSGSREKKIRAARDRFYKGDI
                     ADALNEYYKTSGAFLRKHDLETHETLIEEPVSIHYRGYRVNKCNTWTQGPAFLQSLRL
                     LEKFDLKSMGFFSPDHIHVTVEAMKLAFADRDKYYGDPAFVKVPLKQLLSDKYTGIRV
                     PLIDMKKASAQIRPGDPYAMKALAGPGEYRKGEPGTTTCTVVDKWGNAVAATPSANPE
                     YGVCESLGIAHNTRLCSLNTEKGHPNSLQPGKRPRITLTPTIVLKNGKPVMVMSVAGG
                     EKQDQVSLQLFMDLFDFGMMPKEALSSPRFLSYHMEDSFNPSPDPKARYKNYPRLNID
                     ESVQLDIN"
BASE COUNT         1919 a         1708 c         1873 g         1604 t
ORIGIN      
        1 ggctaccagt ttcaggaagc catgttcccg agcaccaaca ataaaaaata caatctcgcg
       61 ctcaacctgg atgcccaggc tacggcaaca acgaaaatat cctttaatat taacggaagg
      121 agccagaatt cccaatatcc cgccattgca ccgggccggg tcttcgaatt gttgcagtat
      181 gtcaataccc ggcggggagc cttattcttc acgaacggca aacccgggga taaaatacct
      241 gcaacgattt atggcagcgg atatgagaag atcaataccc tggcattgta ttcccagttg
      301 tctattgaac aggacctgcc ttttattccg ggactgaaag caaaaggcac cgtggcctat
      361 gatccgtcta acgggctgga taagctctgg cgtattccaa cgcatatcac cagcattgta
      421 gacacttcac aacatcctta tgtattcaag gacgccatat ttgaccaggc ccaggcttcc
      481 ttaaacgaaa ccaacaggca atcttaccag cttacttacc aggcaagcct gaattatgtg
      541 aaatccttca ggaataacaa tatcaccgca ctggccctgt ttgaagcaaa gagcaatgac
      601 agcctttacc tggcggcttc gagaaggaat tataatgtga atattgatga aatagatctg
      661 ggaagttcca gccaggcgga tatgacgacc aacggatcat cgagtgctac ccgccaattg
      721 ggtctgctct accgggtcac gtataattat tcacagaaat atttttttga ggccagcggc
      781 cggtacgacg gcagctatta tttcgctccc ggaaaacagt ttggtttttt cccggcattt
      841 tctgccggat ggcggctgtc gcaggagaat ttcctgaagg acataagctg gatcgacaat
      901 cttaagatca ggggttctta cggggaagtg ggtgcgctgg caggtgctcc cttccagtac
      961 ctgagtacct acagtgtata cggtccggct tatgtgttgg gcgggcaggc cgtacaggca
     1021 gccagcgaac gggccgaaag caatccgaac attacctggg aaagagcgaa aaaaacagat
     1081 atcggtctgg acctttccct atggaacggt ttgctgaccc tggaagccga ttatttttat
     1141 gagaagagat ccaatatgct ggtgtcgccc aatgtgatcg ttccttcgga atatggaatc
     1201 ggactcagtc aggtgaacgg cggtatcatg gagaataaag gaatcgattt ctcgatcggt
     1261 tctgcctata gcatctcaaa agattttcag atttccctgc agggtaattt cacctatgcc
     1321 aggaacactt tgctgaaagt tttcgaaacg tctgcgacct acgacaaccc gaaccgccgg
     1381 ataaccggca agccgctcgg gacccagttt ggtttccatg ccgtaggata cttccaggag
     1441 gcggacttca atccggacgg aacgcttaaa tccggcatag ccgttcagcc ctggggagcc
     1501 gtaaatcccg gtgatctcag atatgaagac atcaatcacg acgggcagat caataatgat
     1561 gacctgacca agatcggtaa ttctccgacc cccaggatca tttatggaat ttcgcccagc
     1621 gttcggtaca aaggcctttc tctggatctg ttattccagg gcgcagccga tgcaaacctt
     1681 tatatggacc ggcagatgca gtggccattc tataattcaa tgaacgctta cgtcgccaat
     1741 ttcaattcct ggacgccgga taatccgaat gcaaaatatc cgcggatcac caatgcgcca
     1801 accaccaata actcccagca atcatcctgg tggatgacca atgtgagtta tctgcggctg
     1861 aaaagcgcta cgctggcgta tgtcattccc tcctttatca tcagccgcat caaaatgcaa
     1921 agcgtgcggg tttttctgtc tggccagaat attcttacct ggaccaaact gatcaattac
     1981 gatccggaga atgccggcgc ctatacgaat ctttatcctc agcagaaagt tatttcagca
     2041 ggtcttaata tcagctttta acaatcgaaa taaataaaat gccatgagct atatatttaa
     2101 taatgccaga atcgccgtcc tgatactggc gatcacgggc ctggtttcct gtagtaagta
     2161 cctggacgtt accccgaaag accaggtgac caacagcgca ctctggtcaa gcacgggaaa
     2221 tgcggatctg tttttaaaca atatttattc cagcctgccc gatccgtaca ataaactgga
     2281 gtcggaagat acctggacgg acaacgccat ggccggttat aattattatg acagccgggc
     2341 catctatgcg aggtcggtgt atacgccggt caacgctccg aactactggg tcaattacaa
     2401 ttatgtacgt aagtgtaatg tatttataca gaatgtttcc gcttccacca cgctgccgga
     2461 tgcctggaaa aaacaacgcc ttgcggaagc ccggtatctg agggcatatt attatcaatt
     2521 gctgtggaca cattacggcg gcgttccgat catcacggac gtgctgaacg aaaacacgca
     2581 gggcgattcc atcttccggg tgcggaacac ggatgaagag acgtttaaat ttatcgacga
     2641 tgaatgcgct gcgatcgccg atgacctgcc tgcgaaatcg gatgcaggca gagccacaaa
     2701 gggcgcggtg ctgactttaa aaggatggtg cgagttgttt gaggccagtg cgttgaaaaa
     2761 tccggagaat gatccggcga aatgggcgtt ggctgcggcg accaataaac aggtgatgga
     2821 cctgggcgtt tacagcctgt tcccggatct ggaaagcctg ttttttgaag ggaataacaa
     2881 taacagcgaa acaatttttg ccagaaccta tttaggcggc agcgccacac tgggtggaag
     2941 caaggaagga ttgtggggct tacgcaatgt gggcggagcg caggtaggtt atgggggcgt
     3001 gaatccgacc caggagctgg tggatgatta tgaaatggcc aacggccttc ccatcaccga
     3061 tccggcctcc ggatatgatc cgcaggcgcc ctatgttaac cgagagaaaa gattctatca
     3121 gtccatcatt tacgacggct gcacctggct gggctatgtg atccttacca gacggggcgt
     3181 cggcagcaag aatgaaattg atctgaccaa tggctcggga ggcggaccca atacgggcta
     3241 tgcattacgc aaaacactgg atccgaaata cgccgtcagc ggaagtaatc atctgaacag
     3301 tgcgaactat aatatattcc ggtatgccga agtgctgctg agttatgcag aagcccagaa
     3361 tgaagctgtg ggaccggatg cctccgttca ggacgcggtc aaccaggtca gagccagatc
     3421 ggacctgcct gcgttgccga cggacctgac ccaggatgaa atgcggacgg cgatccgccg
     3481 ggagcgccgc gtggaacttt cttttgaaga gaaacgctgg tacgatctga tccgctggaa
     3541 aacggcggaa ataaacctca atacgccttc cctgcattgt atggtcattg aaaaagtaaa
     3601 tgacgtgtgg acttacaccg tagagcctgc agcagatgga agccgtatct tttacgccaa
     3661 taaaaattat gtattgccga ttccgcagga cgcaatagat aaaaacacca aactggtgca
     3721 gaatccgaat tattgaggaa gatatatttg gtcagggaga aggtattcca ctttttaaga
     3781 aggaaattta gcaaatgagg gggatcgggt atgaaggaaa atttgctgat catttaattc
     3841 tttaaaaaag caaaaatgaa taacagaaga gattttctca agcaggtatc cgcattggga
     3901 gcactgagcg ggttgccaaa taccctgtct gcccaatcgg aatccgggcc ggacctgacc
     3961 tggtatgctt ccggcaacgg aggtgttgtg ggaagcgggc cgggcccttc cgcccaggct
     4021 ggaattgaga tgcttaacaa aggaggcaat gccgccgacg gagccgccgc ggctctcttc
     4081 aacctcatgg tttgtgatta cggaaatttc tgtatcggcg gggaagtgcc tttcatgtac
     4141 tataatgtaa aggatggcaa aataaatgtc tttaacggaa tgggcggagc gcccagggat
     4201 ccgaaagcca ttgaatggta ctaccgccat ggcattccga ataaaagagg aattaaagca
     4261 tccaccacac cgagcgcggt aagtacctgc ctgaaggcgc tggaagtaaa agggaccatg
     4321 tcgttcgagc aggtgatcgc ccctacgctt tcgctgctgg acgccggcgg aaagaaatgg
     4381 tatgcgaacc tggcaacgac cttccgaaaa ctggttgaaa ctgaaagaag caccggcgga
     4441 agccgtgaaa agaaaatccg cgcagccagg gaccgctttt acaaagggga tattgcggat
     4501 gaactgaatg attattatat ccgttcggaa ggcttcctgc gaaaatcgga tctcgaagcg
     4561 catgagacgc tcattgaaga tcccgtgtcc atcaattacc gcgggtatga tgtttacaaa
     4621 tgtaatacct ggacccaggg tccggttctt ttgcagacct taaggctcct ggagaatttc
     4681 gatctgaaat cgatggggtt tttatccgcc aattacatac acaccctggc agaggcaatg
     4741 aaactggctt atgcggaccg cgataaatat tatggtgatc cggcctttgt aaaagtgccc
     4801 ttgaaggaat tgctttccga tgagtatacc gcgatccggt ggccgttgat cgataagaat
     4861 tatgcctcgc aggcaatacg gcccggggat ccatacaaaa tgcaggctga tgcgggcgcg
     4921 ggggaatact ggcccgggga atccggtacc accacctgtg tggtcgttga caaatggggg
     4981 aatgtagcgg ctgcgacacc gagcgccaat ccggaatacg ccatttgcga aagcctgggt
     5041 atcgcccata atacccggct cagcagtttg aatacgcaga aaggtcaccc taattcattg
     5101 gagccgggca aacgtccgcg gatcacgctc acgcccacga tcgtgctgaa agatggaaaa
     5161 cccatcatcg cacagagcat caatggcggc gatgtccagg atcaggtatc tttggagctc
     5221 tttcttgatt ttgcagagtt tggtatgatg ccaaaggaag cggtatccgc tccgcggttc
     5281 agaacttacc attttgaaaa ctcgtttaat ccggcctcct atcgggccag ccgtatctta
     5341 agactgggcg gccttacgat aaattccttt aacgaatcga tattggaaac cctccttacc
     5401 cggggacaca gtgtaaacgg ggttaaaggg accatgggga ttccggttat ggcttatatt
     5461 gaccagaaga cgggaatatc ctatgtggcc ggagagccca ggatcaggtt ttgtgccgca
     5521 cggaatctat aatccggcgg ataaaaaatg aacagcatga gtaacagaag aaaatttatc
     5581 aaacagatct ctgcattggg agtgctcggt agtttgccgg atatgctggg ggcacaggcc
     5641 gcgcccggcc aggaattgac ctggtatgct tcgggaagcg gaggggctgt ggcagccggt
     5701 ccgaaggtat ctgcccaggc aggtatcgat atactcgaga aaggtggaaa tgcgattgac
     5761 ggtgcggtag cggttatttt caatctggcc gtttcaaact atggcctgtt ctgtatgggc
     5821 ggcgaatctc ctttcatgtg ctatacggcg aaagatgcca aagtgcatgt atttaacgga
     5881 atgggcgggg cgccaaaaga tccggcagca atagagtggt attataagca tggcattcct
     5941 gatctgttcg gcccggaaaa aggaggaata aaagcggcta cgacgcccag cgcagtgagc
     6001 acctgtctga aagcgcttga aacgaaggga actttttcgt ttgagcaggt gatcgcatca
     6061 accctgtccc tgctggatgc cggaggcaaa agctggtatc cgaacctggc cgttaccctg
     6121 cggaaaatgg tggagacgga aagaaataca agcggcagcc gcgagaaaaa gatccgggcg
     6181 gcccgggatc gtttttacaa gggagatatt gcagacgcgc tgaatgaata ttacaaaact
     6241 tcgggagcct tcctgagaaa acacgatctg gaaacgcatg aaacattgat cgaagaaccg
     6301 gtatccatcc attatcgcgg gtaccgggta aacaaatgca atacctggac gcagggaccg
     6361 gcttttttgc aatccctgcg cctgctggaa aagtttgatc tgaaatccat gggtttcttt
     6421 tcaccggatc atattcatgt taccgtagaa gcaatgaaac tggcttttgc ggaccgcgac
     6481 aaatactacg gggatccggc ctttgtgaag gttcccctga agcaactgct ttcggataaa
     6541 tataccggta tccgcgtacc gctgatagat atgaaaaagg cttcggccca gatcagaccg
     6601 ggcgatcctt atgccatgaa ggctcttgcc ggacccggcg aataccgcaa gggagaaccc
     6661 ggaacaacaa cctgcacggt agttgataaa tggggcaacg cagtagcggc cacccccagc
     6721 gccaatccgg aatatggggt atgcgaaagc ctgggcatag cgcacaatac ccgcctctgc
     6781 agcctgaaca cggaaaaagg acatcccaat tcacttcagc cgggtaagcg tccccggatc
     6841 acccttacgc cgacgatcgt tctgaaaaac ggcaaaccgg taatggtgat gagcgtggca
     6901 ggaggagaga aacaggatca ggtatccctg caattattta tggatctttt cgacttcgga
     6961 atgatgccca aagaagcgct ttcctctccc aggttccttt cctaccatat ggaagattct
     7021 ttcaatcctt cccccgatcc gaaggcgagg tataagaatt accccaggct gaacatcgat
     7081 gaatccgttc agctggatat caat
//
LOCUS       DASYCU010000309         5102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4562491, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000309 DASYCU010000000
VERSION     DASYCU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4562491"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGM24_06255"
     CDS             <1..541
                     /locus_tag="VGM24_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4155006.1"
                     /translation="EGNRLIRQIKENVSNLYSGTRDILWTLNPGSDRLSEIAGRLGSI
                     GIEIFQDTPIEFRFEKRLRSSDDLQLPASYGRNMLMIFKEAMNNSLKYSDASQISLTI
                     EKAEEGELNILLKDNGVGFDPVQAKRGHGTGNMFKRAKRIQSEFIIESRPQCGTACRL
                     ITPLGPGGKGPGPACFGRF"
     gene            665..1003
                     /locus_tag="VGM24_06260"
     CDS             665..1003
                     /locus_tag="VGM24_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY4155007.1"
                     /translation="MKIFVAGLPFAVSDQELQEIFEPYGTVGSAKVILDRETHKSRGF
                     GFVEFASNEEAEKAIKALDGTSLEGRTLTVKVAEDKNPGGGGGGYRGGGGGGNRGGGG
                     GGYNRDRNRY"
     gene            complement(1186..3183)
                     /locus_tag="VGM24_06265"
     CDS             complement(1186..3183)
                     /locus_tag="VGM24_06265"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY4155008.1"
                     /translation="MVIRKKIVPALTLLLLGFRTGLAQVPAAVSPTDTASEFRPAELF
                     SSIFYTSDGNERNGANGSAGPAYWQNRCDYQINAVLDTAQNTLTGSEHIRYTNNSPDS
                     LSYIWLQLDQNTYRPDARSRFAAKSTGGQYTSGYALDSVYLENGRRLEKADYIITDTR
                     MQIRLMHALPSRGGRLQLYIKYHYEVPGSFGNRTDHYKTKNGEVYEIAQWFPRLCVYD
                     AGQGWNTLPFLGAGEFYLEYGDIDYRVTVPWNMIVAGSGELMNPEEVLTAGQVSRLAK
                     AKNSDQTLMIRSPEEVGDPHSRPKQRGLVSWHFRMRNTRDVAFGTSAAYIWDAARVNL
                     PDRKKCLAMSVYPVESAGRDGWGRATEYLKASIEYFSKKWFVYPYPVAVNQAGIAGGM
                     EYPGIVFDGWTDKGKDLYWVTAHEIGHNWFPMIVGSDERRFGWMDEGFNTFIDIYAAD
                     EFHQGEYAPKRDSEYAPGGGNPAEEILPTLRDPEAPVILSPADAISEKYRHPVTYYKT
                     AFGLVLLREQILGKDRFDYAFRKYIQAWAYKHPRPDDFFRSMENAAGEDLSWFWKEWF
                     YNNWEFDPAIAGAAYTGNGYAKGVDIRIVNKKRMVLPLTLELKYRNGTSSRKTIPVEA
                     WLLQRQISVHEDLSSPLASVSIDPDAALPDSDRKNNRIAFP"
     gene            3323..>5102
                     /locus_tag="VGM24_06270"
     CDS             3323..>5102
                     /locus_tag="VGM24_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEY4155009.1"
                     /translation="MDTRREFIKKAGLLSGATGLFSMLPPSIQRALAINPDPGSSFLD
                     AEHVVILMQENRSFDHCFGCLRGVRGFNDPRAITLPDQNPVWLQSNKAGETYAPFHLD
                     IRNTKATWMNSLPHSWSNQVDARNDGKYDQWLNVKRSGNKDYSDMPLTMGFHNRDDIP
                     FYYTLADAFTVCDQHFCSSLTGTSPNRSYLWTGTIRDRQRETSRALVWNDDMDFESLG
                     WRTFPELLEENNISWKIYQNELSVGVGFEGEEDAWLSNFTDNNLEFFKQYHARLNKRH
                     LLYLEKIAVSLPVEIEELKRKLATLPPADPSVEAETKRLKEKQKKWNELQKEMETHHP
                     AAYEGLTPREKSIHQKAFTTNEQDPDFHTLTELTYSDNGVEREMQVPKGDVLYQFRED
                     VRTGALPTVSWLVAPENFSDHPGAPWYGAWYVSEVLDILTQNPELWKKTIFMLTYDEN
                     DGYFDHVPPFVAPHSHKPGTGKVSGGMDTRVEFVTLQQEEERENFPSAYDRESSIGLG
                     YRVPLVIASPWSRGGFVNSEVFDHTSSLQFLEEFLSRKTGRKITETNISDWRRTVCGN
                     LSSVFRPYSGEKTDNPAFLSRDEVLEG"
BASE COUNT         1265 a         1239 c         1336 g         1262 t
ORIGIN      
        1 agaaggaaac cggttgatcc gccagataaa ggaaaacgtg agcaacctat acagtggaac
       61 ccgggatatt ctctggacgc tcaacccggg aagcgaccgc ttgtctgaaa ttgccggcag
      121 gctgggaagt atcgggattg aaatttttca ggatacgccc attgaattca gatttgaaaa
      181 aagacttcgc tcttcggatg atcttcagct tccggccagc tacggacgga atatgctgat
      241 gatatttaag gaagcgatga ataatagtct taaatattcc gatgcatcgc agatcagcct
      301 gaccattgaa aaagcggagg aaggggaact gaatattctg ctgaaggata atggggtcgg
      361 gtttgatccg gttcaggcaa aaagaggcca cggaacagga aatatgttca agcgggcaaa
      421 gcgaattcag tcagaattta tcattgaaag ccggccccaa tgcggaacgg cctgccggtt
      481 gattaccccg ctggggccgg ggggaaaagg gcccggaccg gcctgttttg ggcgctttta
      541 gccttttcgc ctgtcgggag ggcctttatt tcgttaaaag ggatcaataa tgaaaaaaaa
      601 acaggggatt ggatgagcga tcagaactat tgtggtatat tgtcggctaa taaattgtat
      661 tggaatgaaa atatttgtag ccggtctgcc tttcgctgtg agtgatcagg aattgcagga
      721 aatttttgag ccttatggaa ctgtaggttc tgccaaggta attttagaca gagagaccca
      781 taagagccgc ggttttggtt ttgtggagtt cgccagtaat gaagaggctg aaaaagccat
      841 taaagcactc gacggcacca gcctggaagg aagaacgctt accgtaaaag ttgctgagga
      901 taaaaatcct ggcggcggcg gtggtggtta tcgtggcggt ggcggaggtg gaaaccgcgg
      961 aggtggcggt ggtggatata accgcgacag gaaccggtat taattcgctt tagtcttcga
     1021 taccagcata gtttcgctgt tctcaaacct atttacggat ctcagtaacg tattcaggtg
     1081 cccgtaaaat caattttgcc gggcacctga atcttttttg ccataaagaa gctccggtgg
     1141 cctgcatttg cacatcccgg ccgggacacc gtttgtgcgg gagggtcaag gaaaggcaat
     1201 ccggttgttc ttccggtcgg aatccggtaa agcggcatcg ggatcgatgc tgaccgatgc
     1261 caggggagaa gagagatctt catgaacact gatttgccgt tgcagcagcc aggcctccac
     1321 cggaattgtt ttccggctgc tggtcccatt cctgtatttt aactccaggg ttaacggcag
     1381 caccatcctc tttttattga cgatgcggat atccactccc ttcgcatatc cgttaccggt
     1441 ataggcggct ccggcgatcg cggggtcaaa ttcccagtta ttataaaacc attctttcca
     1501 gaaccaggaa aggtcttccc cggctgcatt ttccatgctt cgaaaaaaat catccggcct
     1561 gggatgcttg tatgcccagg cctggatata ttttctgaag gcataatcaa agcggtcctt
     1621 tcccaggatc tgttcccgta ataacaccag tccgaaggcg gttttgtaat acgttacggg
     1681 atgccggtat ttttctgaaa tagcatctgc cggcgacagg atcacgggcg cttccgggtc
     1741 gcgcagcgtt ggcaggattt cctccgcagg atttccgccc cccggcgcat actccgagtc
     1801 ccgcttgggc gcgtattccc cctgatgaaa ttcatccgcc gcatagatat cgataaaggt
     1861 attaaaccct tcatccatcc agccaaaccg gcgttcatca ctaccgacga tcatggggaa
     1921 ccagttgtgc ccgatttcat gggccgtcac ccaatacagg tccttgcctt tgtccgtcca
     1981 accgtcaaag acaattcccg ggtattccat tccaccggca atgcccgcct ggttaacagc
     2041 taccgggtat ggatatacga accatttttt ggaaaaatat tcgatggacg ctttcaggta
     2101 ttcggtagcg cggccccagc catcccttcc ggcactttcg acgggataaa cggacatggc
     2161 caggcatttt tttctgtccg gaaggtttac ccgcgctgcg tcccatatat aggcggcgct
     2221 ggttccaaag gccacgtcgc gggtattccg catgcggaaa tgccagctga ctaaaccccg
     2281 ctgtttcggt ctcgaatgcg gatcgccgac ctcttccggt gaacggatca tcagggtttg
     2341 gtcactgttt tttgctttgg ccagccggct gacctgcccg gccgtcagca cttcttccgg
     2401 attcatcaat tcgccggatc cggcaacgat catattccag ggaacggtaa cccggtaatc
     2461 gatatccccg tattccaggt aaaattcgcc ggctcctaaa aagggaaggg tgttccatcc
     2521 ctgaccggca tcatatacac ataggcgcgg aaaccattgg gcaatctcat atacctctcc
     2581 gtttttggtt ttgtagtgat cggtgcggtt gccgaatgat ccgggaactt catagtggta
     2641 tttgatatat agctgcaaac ggccgccgcg ggagggcaaa gcatgcatga gccggatctg
     2701 catccgggta tccgtaataa tataatccgc cttttccagc cggcgcccat tctccaggta
     2761 aacactgtcg agtgcatacc ccgatgtata ttgaccgccg gttgattttg cagcgaaccg
     2821 ggaacgtgca tcgggacgat atgtattctg atccagctga agccagatat atgataaaga
     2881 atccggactg ttattggtat accggatatg ttcggagccg gttaatgtat tttgtgcggt
     2941 atccagtact gcgttgatct gataatcgca acggttttgc caataggcag gacctgctga
     3001 accatttgcc ccattcctct cgttaccgtc cgaagtataa aaaatggaag aaaagagttc
     3061 agccggtctg aattccgatg ccgtgtccgt cggggagact gctgccggaa cctgtgccag
     3121 gccggttcgg aaaccaagca ggagcagcgt cagggccggt actatttttt ttctgatgac
     3181 catgttgtta aactcaattg aattttattt tttgcaaatg aaggtaagca ttcattgtta
     3241 tttttttttg atcatttgat ccgggagatt gaataactgc gattgattta tatttatgtg
     3301 gtaatatcaa attgactgat ccatggatac gcgaagggaa tttataaaga aagcgggctt
     3361 attgtccggc gcaacggggc ttttcagtat gttgccccct tcgatccaaa gggcgcttgc
     3421 gattaatccg gatccgggca gcagttttct ggatgcggaa catgtcgtca tcctgatgca
     3481 ggagaaccgt tcttttgatc attgtttcgg atgccttcgc ggagtgcgtg gatttaatga
     3541 tccgcgcgcc attaccctgc cggatcagaa tccggtctgg ctgcaatcca ataaggctgg
     3601 cgaaacctat gctccctttc acctggatat ccggaatacc aaagccacct ggatgaattc
     3661 ccttccgcac agctggtcca accaggtgga tgcgcgtaat gatggaaaat acgatcagtg
     3721 gctgaatgta aaacgatccg gcaataaaga ttattccgat atgcccctca cgatgggttt
     3781 tcataaccgc gacgatatac cgttttatta tacactggcg gacgccttca cggtttgtga
     3841 tcagcatttt tgttcatccc ttacgggcac cagccccaac cggtcatatc tctggacagg
     3901 cactatccgg gaccggcagc gcgaaacatc gcgtgccctg gtctggaacg atgatatgga
     3961 ttttgaatcg ctgggctggc ggacttttcc cgaactgctg gaagaaaata atatttcctg
     4021 gaagatatat cagaatgaac tgagcgtggg ggtgggtttt gaaggagagg aggatgcgtg
     4081 gctgtcgaac ttcaccgaca ataacctgga atttttcaaa caatatcatg cccgtctgaa
     4141 caaaagacat ctcctgtatt tggagaagat cgccgtttcc cttcccgttg agatcgaaga
     4201 gctgaaaagg aaactcgcga cccttccccc tgccgatcct tccgtggaag cggaaaccaa
     4261 acggctgaag gaaaaacaaa aaaaatggaa cgagctgcaa aaggaaatgg agacccatca
     4321 ccccgcagcc tatgaaggac tgacgccgcg tgaaaagagc attcatcaaa aagcgtttac
     4381 gaccaacgaa caggacccgg actttcatac gcttacggaa ttaacctatt ccgataacgg
     4441 ggtggaaaga gaaatgcagg tgcccaaagg agacgtgttg tatcaattca gagaggatgt
     4501 ccgaacgggt gccctgccta cggtatcctg gctggtcgcg ccggaaaatt tttcggacca
     4561 tcccggcgct ccctggtacg gcgcctggta cgtgtctgaa gtgctggata ttcttacgca
     4621 gaacccggag ctgtggaaaa aaacgatttt catgcttacc tacgacgaga atgacggata
     4681 ctttgatcat gttccgccat ttgttgcgcc ccattcccac aaaccgggaa cgggtaaggt
     4741 ttccggaggc atggatacgc gggtcgaatt tgtaaccctt cagcaggaag aagaaagaga
     4801 gaatttcccc tctgcctatg accgcgaaag ttccatcggt ctcggttata gggtgccgtt
     4861 agtaatcgct tccccatgga gccggggggg ctttgtaaat tcggaagtat ttgatcatac
     4921 ctcctccctt cagttcctgg aggaatttct cagcagaaaa acaggcagaa aaattacgga
     4981 gaccaatatc agcgactggc gccgcaccgt ttgcggaaac ctgagctccg tcttccggcc
     5041 ttatagcggg gagaaaacag acaaccccgc cttcctctcc cgggacgagg tcctggaagg
     5101 ta
//
LOCUS       DASYCU010000310         5317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1953478, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000310 DASYCU010000000
VERSION     DASYCU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953478"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VGM24_06275"
     CDS             <1..188
                     /locus_tag="VGM24_06275"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HEY4155010.1"
                     /translation="RYFSLTGKLLDSRLTLSQIIALRFAPDNEFVELADRALKENLNN
                     SSIKKAIRQWKADHHRV"
     gene            complement(207..473)
                     /locus_tag="VGM24_06280"
     CDS             complement(207..473)
                     /locus_tag="VGM24_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155011.1"
                     /translation="MNQFVIDFIYNGAPYSGLVTPKEENNSTYYSVHLESENQEVHLD
                     IEARPCDADQMDWCFRCPDGQEPPKNYDKAFLQEIGEAIEKCEA"
     gene            complement(532..984)
                     /locus_tag="VGM24_06285"
     CDS             complement(532..984)
                     /locus_tag="VGM24_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155012.1"
                     /translation="MKKTNEAASDLTDPKKDREKLKGDRGTLDLPELKDIPGQKKKGL
                     SAGVPPGDTTISSADEEGDDVLGNEDEDAFVSPQEEELPDQSFDPPYDADLPVEEISL
                     DDRDSEGERLEESGPEQDLFGEDLDDDLTEEEDEESEGENEKGDGGKA"
     gene            complement(1061..2227)
                     /gene="dinB"
                     /locus_tag="VGM24_06290"
     CDS             complement(1061..2227)
                     /gene="dinB"
                     /locus_tag="VGM24_06290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY4155013.1"
                     /translation="MDNERHIAHLDLDTFFVSVEKLRNPKLAGKPILIGGSNDRGVVA
                     SCSYEARKFGIHSAMPMYMARRLCSHAIVIRSDFEAYSKYSAIVTDIIKDTVPVFEKA
                     SIDEFYVNLTGMDKFHGCKKFTDELRIRVIRESGLPVSYGLASNKLISKVATNEAKPN
                     GQLEIAPGFEKSFLAPLSIMKIPGVGTKTGYKLLKMGIETVKKLSEIPVHLMQSFMGE
                     SGVELWRRAQGIDESPVIPYREQKSISTERTYQRDTIDVDFLMGDLYRMTEKIAFELR
                     KQNRLTGCVTVKIRYTDFETHDIQKTIPYTNADHELFRVAHELFTRLFERRQLIRLIG
                     IRFTHLIPGTYQINMFEDREEMIKLYQSIDHIKTRFGEGLLVRGRCLFPNRNGV"
     gene            complement(2232..5183)
                     /gene="dnaE"
                     /locus_tag="VGM24_06295"
     CDS             complement(2232..5183)
                     /gene="dnaE"
                     /locus_tag="VGM24_06295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY4155014.1"
                     /translation="MYLNCKTNFSFLYGTFSTRELIQTAVEHGITSLALTNINSTCDS
                     WEFVKLCREAGIKPIPGVEIRNGDKLLYILLAANNKGLAWMHSFLSEHLLEKKDFPES
                     GQSFFPDSWDGFVIYPFGAKDPEELVPNERIGLLPTETGRLFGFDLRRHSDKLVVRQP
                     VTIQDKQHFSLHRLLRCISKNTLLAKLSPQEQCDPSETFLSRDELLSKFKSFPFIVIN
                     TYKLMDECRIEMDFGKDKSKNTYGGTKEDDRIFLEKLATEGLKKRFGGRNRIAEERLK
                     KELKIINDLGFNSYFLINWDFIRYAQLREYYYVGRGSGANSLVAYCLFITNVNPIELN
                     LYFERFLNPHRTSPPDFDIDFSHTDRDDVMDYIFKRYGRNQVALLGSYPTFKHDSVIR
                     QLGKVFGLPDQEIVDLQRTGRATDQNGRLILQYGRLMQGFPSYPSLHPCGMLISQEPV
                     MNYASLFMPPKGFPTAQIDMFSAEDIGLNKFDILSQRGLSHIREAQRLIKLNKGVSID
                     IHDVEKFKQDREVATRIREADSIGCFYIESPAMRQLLKKLKCDDYITLVAASSIIRPG
                     VAQSGMMKEYIYRYHHRDRIKYLHPLFEEHLSETFGVMVFQEDVIKIAHYYGGLDLGK
                     ADILRRAMSGKYRSNNQFRMIREEFFASSKKLGREEALTAEIWRQMESFAGYSFNKAH
                     SASFAAESYMSLYLKTYYPKEFMVAVINNFGGFYSTELYFLELLKTGGEIEPPCINNS
                     DEYTNIQGNKVYVGFVHIKRLQDKLAETILEERRNNGNYLGLQDFIERTNTGIEQLNT
                     LISIGAFRFTGKSKKQLLWEANFLQKKNQPQLHNGPALFREPPVEFTLPELLDTRLDD
                     LYDQIEILGFPLSNPFELADDDPGKYIRATDLENNKGKTVSALTYFINRKHVVTKYND
                     EMFFGTFVDSELNWIDTVHFPDSARNFPLHDSGFYRIRGKVAEDFGVYSIEVQQMEKV
                     GHKKRIYENLG"
BASE COUNT         1255 a         1362 c         1267 g         1433 t
ORIGIN      
        1 taagatattt ctctttaaca ggcaaattgc tcgacagccg cttaaccctt tcccaaatca
       61 tcgccctccg gtttgcgccg gacaatgagt tcgtggaact ggcggaccgc gctttaaagg
      121 aaaacttaaa caacagctcc atcaagaagg ctatccgcca atggaaggcg gaccatcacc
      181 gggtctgaag gctgccgcgg acaggttcat gcttcgcatt tctcaatcgc ctcaccgatt
      241 tcctgtaaaa aggccttatc atagttcttt ggcggctcct gaccgtccgg gcacctgaag
      301 caccagtcca tctggtccgc atcgcagggc ctcgcctcga tatccagatg gacttcctga
      361 ttttcactct ccaggtgcac ggaataataa gtcgaattat tttcttcctt cggcgtgacc
      421 aggccggaat aaggtgcccc gttatagata aaatcaatca caaattggtt catcggcaaa
      481 tcttttctga tacaatttac caaattttcc gcccattgcc tgaccggcag atcaggcttt
      541 tccgccatcg cctttttcat tttccccttc gctttcttca tcttcctctt ctgtcaggtc
      601 atcatccagg tcctcgccga aaagatcctg ttcaggcccg gactcttcca gcctctcccc
      661 ctcgctgtcg cgatcatcca ggctgatctc ttctacgggc aggtccgcat cataaggcgg
      721 atcaaaggac tggtccggca actcctcttc ctgcggtgaa acgaaggcgt cttcgtcctc
      781 gttacccagc acatcatctc cttcttcatc cgcggatgaa atggtcgtat ctcccggcgg
      841 gactccggcg gacaggcctt tctttttctg cccgggtata tcttttaatt caggcagatc
      901 aagcgttccg cgatcccctt ttaatttttc ccggtctttt tttggatccg ttaaatcaga
      961 tgccgcctca tttgtctttt tcataatact gattttacag aatgcaccta aatttccgtg
     1021 ccatcggatt ggtctgccaa aaaaacaggg actggttatt tcatacccca ttccggttcg
     1081 ggaacaggca tcttccccgg accagaagcc cttctccaaa ccttgttttt atatggtcga
     1141 tcgactgata cagcttgatc atttcttccc ggtcctcgaa catattgatc tggtaggtgc
     1201 ccgggatcag gtgagtaaag cggatcccga tcagccggat cagctggcgg cgttcgaaaa
     1261 gccgggtgaa cagttcatgg gcgacccgga ataactcgtg atcggcattg gtatacggaa
     1321 tcgttttctg gatatcgtgg gtctcaaaat cggtgtaccg gatcttcacc gtaacacagc
     1381 cggtcagccg gttctgtttg cggagctcga aagcgatctt ttcggtcatg cggtaaagat
     1441 cgcccatcaa aaaatccacg tcgatggtat cgcgctgata ggtcctttcc gtggaaatgg
     1501 atttctgctc ccggtaaggg atcaccgggg attcgtcgat gccctgggcg cggcgccaga
     1561 gctcgactcc gctttctccc ataaaggact gcatcagatg taccgggatt tcgcttaatt
     1621 ttttgacggt ctcgataccc attttaagca atttgtaacc ggttttggtc ccgactcccg
     1681 gtatcttcat aatgctgagg ggggcaagaa atgatttttc aaagccgggg gcgatctcca
     1741 gctgtccgtt gggtttggct tcgttcgtgg cgaccttgct gatgagttta ttgctggcca
     1801 ggccatagga aaccgggagg ccgctttccc tgatcacccg gatcctgagc tcatccgtaa
     1861 attttttaca gccatggaat ttatccatgc cggtcaggtt tacgtagaat tcgtcgatgg
     1921 aagctttttc gaaaaccgga acagtatctt ttatgatgtc cgtgacgatc gccgaatatt
     1981 tgctgtacgc ttcgaaatcg ctcctgatca cgatggcatg ggagcacaac cgcctggcca
     2041 tatacatcgg catggcgctg tggataccga atttgcgcgc ttcataactg caggaggcca
     2101 ccactccccg gtcgttactg cctccgatca aaatgggttt gccggccagt tttggattac
     2161 gcaatttttc aaccgataca aagaacgtat cgagatccag gtgggcaata tgtctttcgt
     2221 tatccatgcg cctaacccag gttttcgtaa atccgttttt tatgcccaac cttttccatc
     2281 tgctgcactt caatactgta tacgccaaag tcttcggcca cttttccccg gatccggtaa
     2341 aagccgctgt cgtgcaaggg gaaatttctc gcggaatccg gaaaatgaac cgtatctatc
     2401 cagtttaatt ccgaatcgac aaaagttcca aagaacattt cgtcattgta tttcgttacg
     2461 acgtgcttgc gattgataaa ataagtgagg gcggatacgg tcttcccctt attgttttcc
     2521 aggtctgtcg ccctgatata cttcccggga tcatcatcgg ccagttcaaa cgggttggac
     2581 aacgggaagc ccagaatttc aatctgatca tacaaatcgt ccaaccgggt atccagcaat
     2641 tccggaaggg taaattcaac gggcggctcc cggaaaagcg ccggaccgtt gtgcagctgc
     2701 ggctgatttt tcttttgcag gaaattggct tcccaaagca actgtttttt ggatttaccg
     2761 gtaaaccgga aggcgccgat gctgatcagg gtattgagtt gttcgatacc ggtattggtg
     2821 cgttcaataa aatcctgcag acccaggtaa tttccattgt ttctgcgctc ctccaggatc
     2881 gtttctgcca gtttatcctg caggcgtttg atatgaacaa agccaacata caccttgttg
     2941 ccctgaatat tcgtatactc gtcgctgtta ttaatgcagg gaggctctat ttccccgccg
     3001 gttttcaaca gctccaggaa atataattcg gtcgaataaa aacccccgaa attattgatc
     3061 acggcaacca tgaactcctt cggataatac gtcttcaggt aaaggctcat gtaactttcc
     3121 gcagcaaagc tggccgaatg cgctttgttg aaggagtatc cggcaaagct ttccatctgc
     3181 cgccagatct cggcggtaag ggcttcctcc cgcccgagtt ttttcgaact ggcaaagaac
     3241 tcttcccgga tcatccggaa ctggttattc gagcggtact tgccggacat ggcccggcgc
     3301 aggatatcgg ccttgcccag atcgagcccg ccatagtaat gagcgatctt gatgacatct
     3361 tcctggaaga ccatgacgcc aaaggtttcg gaaagatgtt cttcaaaaag cggatgaaga
     3421 tattttatcc ggtcgcggtg atggtaacgg taaatatatt ctttcatcat gccggactgg
     3481 gcaacgccgg gacgaatgat ggaacttgcc gcaaccaggg tgatgtagtc gtcgcatttc
     3541 agtttcttca gcagctgccg catggccggg gactcaatat aaaaacagcc gatggaatcg
     3601 gcttcgcgga tccgggtggc cacctccctg tcctgcttga atttttcgac atcatgaata
     3661 tcaatactga cccccttatt taatttgatc agccgctggg cttcccggat atgactcagc
     3721 ccgcgctggc tcaggatatc gaatttgttc agcccgatat cttccgcact gaacatatcg
     3781 atctgcgcgg tgggaaagcc tttgggcggc ataaaaagcg atgcatagtt catgaccggt
     3841 tcctgggaga tcagcatgcc gcagggatgc agggacggat aactgggaaa cccctgcatc
     3901 agccggccat actggaggat cagcctgccg ttctggtctg tcgcccttcc ggtccgctgc
     3961 aaatccacaa tctcctgatc cggaaggccg aatacttttc cgagttgccg gataaccgaa
     4021 tcgtgtttga aagtgggata agatccaagc aaggcaacct gattcctgcc gtaacgcttg
     4081 aaaatataat ccatcacatc atcgcggtcc gtatgcgaaa agtcaatgtc gaaatccggc
     4141 ggggaagtcc ggtgcggatt caggaaacgt tcaaaatata agtttaattc aatcggattt
     4201 acgttcgtga tgaaaagaca ataggcgacc agcgagttgg ctccgctgcc ccgccccacg
     4261 taatagtatt cccgcagctg ggcataccgg ataaagtccc agttgattaa aaagtaactg
     4321 ttgaacccga ggtcgttgat gattttcagt tcttttttta acctttcttc cgcaatcctg
     4381 tttctgccgc caaagcgttt cttcagtcct tccgttgcca gtttctccag gaagatccgg
     4441 tcatcctcct ttgtgccgcc atatgtattt ttgcttttat ccttaccgaa gtccatttcg
     4501 atccggcact cgtccatcag cttgtacgtg ttgataacga tgaaggggaa agatttgaat
     4561 ttgctcagga gctcatcccg ggaaagaaag gtttcggagg ggtcgcattg ctcctgcggg
     4621 gatagtttgg ccaaaagggt attctttgaa atgcagcgaa gcaggcggtg caaagaaaaa
     4681 tgctgtttat cctggatggt gaccggctgg cggacgacca gtttatctga atgccggcga
     4741 agatcaaagc cgaataacct gccggtttcc gtgggcaata aaccgatgcg ctcatttgga
     4801 accagctctt ccgggtcctt tgctccaaac ggataaataa caaacccatc ccagctatcc
     4861 ggaaaaaagg actgaccgga ttcgggaaaa tcttttttct ccagcaggtg ttcggataaa
     4921 aaggaatgca tccaggcgag gcctttatta tttgcagcga gcaaaatata aagcagctta
     4981 tccccattgc ggatttccac cccgggaatc ggtttgatgc ccgcttcgcg gcagagtttc
     5041 acaaattccc aggaatcgca ggtggaattg atattggtca gggcaaggga ggtaatgcca
     5101 tgttctacgg ccgtctgaat cagttcccgg gttgaaaaag tgccgtaaag aaaactaaaa
     5161 ttggtcttgc agttcaaata catagccctg caagaatact aatttattta gttatttcat
     5221 actaatttta ttagttactg gttttcagct ttttaataag agggtaagga atactgcctg
     5281 aaatttagcg gaataacgct taggggtaaa catttac
//
LOCUS       DASYCU010000311         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2677158, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000311 DASYCU010000000
VERSION     DASYCU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677158"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VGM24_06300"
     CDS             complement(<1..684)
                     /locus_tag="VGM24_06300"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY4155015.1"
                     /translation="MRYLFCFLFIFFQYPGFAQSFTRIHNKAILVDTHNDFLSKAVEN
                     HYAFDSDLKGITYSDLGRMKAGGVDVQVFSIFCDEHFGRGSAFAYANRELDSLFAIAA
                     RNPDRMQIVYSPKELMKVVKRHKLAALSGVEGGHMIEDDLSYLDSFYRRGVRYMTLTW
                     NNSTSWATSAADESKHSFVVTPYGLNEFGKQVVKRMNALGMMIDVSHNGEKTFWDVMR
                     TTTKPVIASH"
     gene            736..1092
                     /locus_tag="VGM24_06305"
     CDS             736..1092
                     /locus_tag="VGM24_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY4155016.1"
                     /translation="MDPEIIRKYCLKKRAVKESFPFGESTLVFKVSGKIFLLLSLDAV
                     PLQFNVKCDPEQAEIFREEYPSVIPAYHMNKKHWNTIILDGRISSRIVWEMIDASYLL
                     VVKTLPLKERKKLDPL"
     gene            complement(1087..1659)
                     /gene="nadD"
                     /locus_tag="VGM24_06310"
     CDS             complement(1087..1659)
                     /gene="nadD"
                     /locus_tag="VGM24_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEY4155017.1"
                     /translation="MKVGLYFGSFNPVHHGHLIIANHVVQTTELDQAWFIVSPQNPLK
                     PSSALLNEYHRLYLVRAAIEGENHLRASEIEFKLPRPSYTIDTMTYIREKYPQHEFSI
                     ILGSDGYQNLPRWKNHEQLLKTAPFYIYIRAGYENLPEYPGAQTFILDAPLLQISSTH
                     IRNILKEGKSIRYLVPDIVKEEIERNRYYL"
     gene            complement(1720..1950)
                     /locus_tag="VGM24_06315"
     CDS             complement(1720..1950)
                     /locus_tag="VGM24_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEY4155018.1"
                     /translation="MKRLSELRSGSKARIHSFEKNDVFLKLMEMGCVPGEIIKVEKIA
                     PLGDPISIAVAGYHLSLRLDEAENIFVDPILS"
     gene            2045..>2397
                     /gene="ybeY"
                     /locus_tag="VGM24_06320"
     CDS             2045..>2397
                     /gene="ybeY"
                     /locus_tag="VGM24_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY4155019.1"
                     /translation="MVPCPLSGRKRLKAFISGLFRLEKHQLKELSYVFCSDPYLLALN
                     RKFLDHDYYTDILTFPSSYGAGKGVSGEIYISVDRVRENAKNLGHSFRMELHRVIFHG
                     ALHLCQYKDKTKKDKA"
BASE COUNT          635 a          555 c          555 g          652 t
ORIGIN      
        1 atgagacgcg atcaccggtt tggtcgttgt gcgcatcaca tcccaaaaag ttttttcccc
       61 gttgtgggat acatcgatca tcattccgag ggcattcatt ctttttacga cctgctttcc
      121 gaattcattc agaccatagg gggtaaccac aaaactgtgt ttgctttcat ccgcggcgga
      181 agtggcccag gacgtactgt tgttccaggt cagggtcata tatcgcaccc cgcgccggta
      241 gaagctgtcg agataagata gatcatcttc aatcatgtga ccgccttcca cgccggacaa
      301 ggccgccagc ttatgccgct taaccacctt catcagctcc ttcggggaat agacgatctg
      361 catgcggtcc ggattgcggg cggctatcgc gaacaacgaa tcgagctccc ggttggcata
      421 ggcaaaggca gaaccccggc caaaatgctc atcgcaaaaa atcgagaaga cctgcacatc
      481 cacgccgcct gctttcattc tgcccaggtc ggaatacgtt atccctttca gatcggaatc
      541 aaacgcatag tgattctcca cagccttact caggaagtcg ttgtgagtat ctaccaaaat
      601 agctttgtta tgaattctgg tgaatgactg ggcgaaaccg gggtattgaa aaaaaataaa
      661 caggaaacaa aacaggtatc tcatatttca tcgatttatt cggaactttc ctaaatgtaa
      721 accatttgac ctgcaatgga tccggagatc atcagaaaat attgtctgaa gaaaagagcg
      781 gtaaaggaat catttccctt cggagaatcc accctggtat ttaaagtgag cgggaaaatt
      841 tttttgctgc tgagcctcga tgcggttccg ctgcagttca atgtgaagtg tgatccggaa
      901 caggctgaaa ttttccggga agaatatccg tctgtgattc ccgcctatca catgaacaag
      961 aaacactgga atacgattat tttggacggc cggatttctt cccggattgt ctgggaaatg
     1021 attgacgctt cttatttgct ggtggtaaaa acgcttccgc tcaaagaaag aaaaaaactg
     1081 gaccctttat aaataatacc ggttcctttc aatttcttct ttaacgatat ccggtaccag
     1141 gtaccggatc gacttccctt ctttcagaat gttacggata tgggtagacg aaatctgcag
     1201 gagaggggcg tctaaaataa acgtctgcgc tcccggatat tcgggcaggt tttcatatcc
     1261 ggcccggata taaatataga aaggggcagt cttcaacagc tgctcatggt ttttccacct
     1321 gggcagattc tggtagccgt cactgcccag gataatagag aattcgtgtt gggggtattt
     1381 ttcccggata taggtcatcg tgtcgatggt gtaagaaggc ctgggtaatt taaattcaat
     1441 ttcagaagcc cttaaatggt tctctccctc aatggctgcc ctgaccaggt aaagccggtg
     1501 gtattcattc aaaagggcgc tggaaggttt taacggattt tggggggaaa cgataaacca
     1561 ggcctgatcc agttccgtgg tctggacgac gtgattggcg atgatcagat gcccgtgatg
     1621 aaccgggtta aaagaaccaa aatataaccc aactttcata caactagtta taaatcagat
     1681 aattatatct ccttttggag cttggccgta tatgtttttt caggaaagaa tggggtctac
     1741 gaaaatgttt tctgcctcgt ccagtcgcag gctgaggtga taccccgcca ctgcaatgga
     1801 aatcggatct ccgagcggag cgatcttttc caccttgata atctcgcccg ggacgcagcc
     1861 catttccatt aacttaagga aaacatcatt tttttcaaat gaatgaattc tggcttttga
     1921 cccgcttctt aactccgaaa gtcgtttcat gtgttggaac aaaagtagct ttgttctctc
     1981 aatgaacttt acgattttcg gaaatgacat gatctcagca ggctccccgg tacaatttca
     2041 ttttttggtt ccctgtccgc tttccggacg caaaagactc aaggcgttta tctccggact
     2101 ctttcgcctg gaaaaacacc agctgaagga gctttcctat gttttctgca gtgatccgta
     2161 tttgcttgcc cttaaccgca agttcctgga ccacgattat tatacggaca tcctgacgtt
     2221 tccttcttcc tatggcgccg gaaaaggggt ttccggagaa atttatataa gcgtagaccg
     2281 cgtcagggaa aatgcaaaaa atctcgggca ttcatttcgg atggaattgc accgggtcat
     2341 ctttcatgga gcgcttcacc tttgtcagta taaggacaag acaaaaaaag acaaggc
//
LOCUS       DASYCU010000312         4790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2822865, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000312 DASYCU010000000
VERSION     DASYCU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2822865"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VGM24_06325"
     CDS             complement(<1..373)
                     /locus_tag="VGM24_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155020.1"
                     /translation="MDNINKEIAALFAGFRREPLVSIKKIPQSGSIRTYYRVHSAEQS
                     YIATYGINIRENLSFIRFSRHFKSRDCPVPEIYAVNEAGTLYIQEDFGDISLLNKLEE
                     NGFGPYVFDLFCMSLKSLARLQ"
     gene            complement(399..3071)
                     /locus_tag="VGM24_06330"
     CDS             complement(399..3071)
                     /locus_tag="VGM24_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEY4155021.1"
                     /translation="MNAIMTIEQKRLVLNSYRKIPLEQWGPYLSERQWGTVREDYGPY
                     GDAWNYFPYDHAHCRQYQWGEDGLAGISDFFQNLCFAISLWNGKDPVLKERLFGLGNN
                     QGNHGEDVKELYYHLDNIPSHYYMEYLYKYPQQEFPYDQIRQENQRRTRSDPEYELLD
                     TGIFDHDEYFDVLVTYAKENAKDIYIRIEITNRFGEAADITILPTLWFYNKWNNDPEK
                     KPRISQRNKNSVKAEHPRLGSYYLYFQSPKDKWYTENETNLEKVSGIPNETIFVKDAF
                     HDAVIQGKNAAELRKIKKGTKFSPLYQFHIEGGKTQTLFLRLSNKSIGNPFTFGFEKI
                     FQQRREEADAFYEEVFGPQPDPRLKRLQRQAVSGLLWSKQYYHFDVEKWLSASDGITP
                     VNAGKLSGRNHNWKHLKNQDIIAMPDKWEYPWYAAWDMAFQSIPMSMVDPNFAKNQLL
                     LIMREWYMKPDGQLPAYEWNFSDVNPPVQAWAALQVYRIEKEKSGYSDTAFLKRIFQK
                     LLINFTWWVNRKDPSGNNMFEGGFLGLDNIGVFNRSIELPGQMQLEQADGTSWMGMYA
                     LNMMDMALEIAMKDVSFEDTATKFFEQFVMIAEALNEHGMWNEEDKFFYDTLAIAGSA
                     PLQLRIHSIVGITTLFAVSIIEKKKLDKLVDFKKRISWFENYRLKNKKFWPNEEKTDG
                     DTILLSLIPRERLVNLLERLLNEDEFLSPGGIRALSKYHEKNPYSVTIENINYTIQYD
                     PGDSTSNLFGGNSNWRGPVWMPINYMIIQSVRKYGQFYGNALKVECPKGSGNYLNLEE
                     VADFLTSRVISLFQQDAGGDIPQFGKYNWFYQKPGNEHLNLFYEYFHGDNGQGLGACQ
                     QTGWTALLAELINEQQRSQKEKIPAETPEITVVN"
     gene            3183..3743
                     /locus_tag="VGM24_06335"
     CDS             3183..3743
                     /locus_tag="VGM24_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY4155022.1"
                     /translation="MGKLSGKKVAILTENGFEESELTSPRKALTDAGAIVHIVSPRKG
                     KVKAWNHDHWSVELPVDVNLAEADAEDYDALMVPGGVMNPDQMRANPDCVDFAQQFLE
                     EGKPVAAICHGPQLLIETGMLSGRNMTSFPSIRTDLENAGVNWADREVVTDQGLVTSR
                     SPADLEAFNKKMIEEIAEGAHAPSAS"
     gene            complement(3830..4627)
                     /locus_tag="VGM24_06340"
     CDS             complement(3830..4627)
                     /locus_tag="VGM24_06340"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY4155023.1"
                     /translation="MRRSFLLLCILIFGCRTNPYRSTNRSYKKQVKQFNHTLKERPPN
                     YFPGQTAEWAAAVNFDLRKPDFIIIHHTAELGCPMTLHTFTVKHSKVSAHYVICKDGT
                     VYHMLNDYLRAWQAGASKWGHNTDINSCSIGIELDNTGFEDFTAPQINSLLMLLDTLK
                     VKYRIPPENIIGHGDIAPGRKVDPNWRFPWKILADHGFGEWYDTSRVIVPANFDPFQA
                     LRTIGYNLKDTVAVVKAFRRHWMQDTVCQVMDSASDKILFDLSLKYQ"
BASE COUNT         1184 a         1168 c         1150 g         1288 t
ORIGIN      
        1 tctgcaaccg ggccaggctt ttcaggctca tgcagaataa atcaaataca taagggccga
       61 atccgttttc ttccagttta ttcaggaggg aaatatcgcc gaagtcttcc tggatgtaca
      121 aagtccccgc ttcattcacg gcgtagattt ccggtaccgg acaatccctg cttttgaaat
      181 gacggctgaa gcggataaaa ctcaggtttt ccctgatatt aattccataa gtggctatat
      241 aactttgctc cgcgctgtga acccggtaat aggtgcggat gcttccggat tgcgggatct
      301 tcttaataga aaccagcggc tccctgcgga agccggcaaa aagggcggcg atctctttat
      361 taatattatc catagtgatt gccgaaacta ctacaaggtc agttaacaac tgtgatttct
      421 ggggtctccg ccgggatttt ttctttctgg ctccgctgct gctcattgat cagctcggcc
      481 agcagggcag tccagccggt ctgctgacag gccccgaggc cctgcccgtt gtctccatga
      541 aaatattcat aaaaaagatt caaatgctca ttccccggct tttgataaaa ccaattgtat
      601 ttaccaaatt gaggtatatc cccgccggcg tcctgctgaa acaggctgat cacccggctg
      661 gtgaggaaat cagccacttc ttccaggtta agataattgc cggatccctt cggacattcc
      721 actttcaatg cgttcccgta aaattggccg tacttccgca cagactgaat gatcatataa
      781 ttgatcggca tccagacggg tccccgccag tttgaattgc ctccaaaaag attggaagtg
      841 gaatcccccg gatcgtattg aatggtgtaa ttgatatttt caatggtcac ggaatagggg
      901 ttcttttcgt ggtatttgga aagggcgcga attccgccgg gagaaagaaa ttcatcttcg
      961 ttcagcagtc tttccagcag gttcacgagt cgttcgcgcg ggataagcga caaaagaatg
     1021 gtatccccgt cggttttttc ttcgttgggc cagaactttt tattcttcag ccggtaattc
     1081 tcaaaccagc tgatccgctt tttaaaatca accagtttat cgagcttttt cttttcaata
     1141 atggaaacag caaataaagt ggtgatgccc acgatggaat gaatgcggag ctggagcggg
     1201 gccgaaccgg caatggccag ggtgtcgtaa aaaaatttat cttcctcgtt ccacatgccg
     1261 tgctcattca atgcttcggc gatcatcaca aactgctcaa agaatttcgt ggccgtatct
     1321 tcaaaagaga catccttcat ggcgatctcc agcgccatat ccatcatgtt cagcgcatac
     1381 atacccatcc agctggtgcc atctgcctgt tcgagctgca tttgccccgg cagctcaatg
     1441 cttcggttaa agacgccgat attgtccagg cccaggaacc cgccttcaaa catattgttg
     1501 ccgctcggat ctttccggtt aacccaccag gtgaagttga tcagcagctt ttgaaagatc
     1561 cgttttaaaa aggcggtatc cgaatacccg ctcttctcct tttctatccg gtaaacctgc
     1621 agggcggccc aggcctggac cggcgggttc acatcgctga aattccattc ataagcggga
     1681 agctgtccgt cgggtttcat ataccattcc cgcatgatga gcaacaactg atttttcgca
     1741 aagttggggt ccaccatgga catgggaata ctttggaaag ccatgtccca ggccgcatac
     1801 caggggtact cccacttatc gggcatggcg atgatatcct gatttttcag atgcttccag
     1861 ttatggttcc ggcccgacaa ttttccggca ttcaccgggg tgatcccatc actggcggac
     1921 aaccacttct ccacatcaaa atgataatac tgtttgctcc acagcaatcc cgaaacggcc
     1981 tgccgctgaa gccgtttcag ccggggatcc ggttgggggc caaacacttc ttcataaaag
     2041 gcatccgcct cttccctgcg ttgctggaaa atcttttcaa atccaaacgt aaatggattg
     2101 ccgatggatt tgttggacag ccgcagaaaa agcgtttgtg tttttcctcc ctcaatatga
     2161 aactggtaca aaggggaaaa tttggttccc ttttttatct ttcttaactc ggccgcattt
     2221 ttcccctgga tgacggcatc gtgaaaagcg tcttttacaa agatggtttc gttcggtatg
     2281 cccgagacct tttcgagatt ggtttcgttt tccgtatacc atttatcttt cggcgactgg
     2341 aaatacaaat aataactgcc cagccgcgga tgttccgcct tgactgaatt tttattgcgc
     2401 tgactgatcc ggggtttttt ttccggatca ttattccatt tattatagaa ccatagggta
     2461 ggaagaatgg ttatgtccgc tgcttccccg aaccggttcg tgatttcgat ccggatataa
     2521 atatcctttg cattttcttt ggcataggtg acgagcacat caaaatactc atcgtggtcg
     2581 aaaatgcccg tatcgaggag ttcatattcc ggatcactcc gggtgcgcct ctggttttcc
     2641 tgccggatct ggtcgtacgg gaattcctgc tgggggtatt tatacaggta ctccatataa
     2701 taatgggagg gaatattatc cagatgatag tataattcct ttacatcttc cccgtggttg
     2761 ccctgattat tccccagtcc gaataatctt tcttttaaaa ccggatcctt tccattccat
     2821 agggaaatgg caaaacacag gttctgaaag aaatcagaaa tacccgcaag cccgtcttcg
     2881 ccccactggt attgcctgca atgggcatga tcatagggaa aataattcca ggcgtccccg
     2941 taaggaccgt aatcttcccg gacggttccc cattgccgtt cgctgaggta gggtccccat
     3001 tgttccagcg gtatttttcg gtaagaattt aaaacaagtc gtttctgctc aatggtcata
     3061 atagcattca tccggtttaa aattacgatt tagttgatta agccgttgaa ggaaccggcc
     3121 tggtatcgtt tttgaatcca gaagaagaag aacaaaacct attcatcatt aaatcaatcc
     3181 cggtgggaaa gctaagtggt aaaaaggtag ccatccttac ggaaaatggc tttgaagaat
     3241 ctgaattgac gagtccccgg aaagcgctga cggatgcggg agcaatcgtg catatcgttt
     3301 ctccccgaaa aggcaaggtg aaggcgtgga atcatgatca ctggtctgtt gagctgcccg
     3361 ttgatgtgaa cctggctgaa gcggatgcgg aagattacga cgcactgatg gtgcccggcg
     3421 gggtaatgaa tcccgatcaa atgcgcgcca atcccgattg tgtggatttt gctcaacaat
     3481 ttctggaaga aggaaaaccc gtagcggcca tttgccacgg ccctcaatta ttaattgaaa
     3541 ccggcatgct gagcggaaga aatatgactt ctttcccttc catcagaacg gatctggaaa
     3601 atgcaggcgt caactgggcc gacagggaag tggtgaccga ccagggactg gttaccagcc
     3661 gcagcccggc ggacctggaa gcttttaaca aaaaaatgat tgaagagatc gcggaagggg
     3721 cgcatgctcc gtctgcttcc tagtggatcc ggcccgtttc tatatacact gctctatcgt
     3781 aaaaaaagaa ccaccgcaat ggtggttttt ttttgaattg cagacgaagc tattggtatt
     3841 tcagggaaag gtcaaataaa attttatccg aagcgctgtc catcacctgg cagaccgtgt
     3901 cctgcatcca gtgccgccgg aacgctttca ccacggcgac cgtatctttc agattataac
     3961 caatggtcct caaagcctgg aaaggatcga agtttgccgg tacgatcacc cggctggtat
     4021 cataccattc accgaatcca tgatcggcca gtattttcca gggaaaccgc cagttgggat
     4081 ctacttttct accgggcgca atatcaccgt gaccaataat attttccggg ggaatccggt
     4141 actttacttt aagcgtatcc agcagcatca gcaaactatt gatctgcggt gcggtaaagt
     4201 cttcaaaccc cgtgttgtcc agttcaatgc cgatgctgca ggaattgata tcggtattgt
     4261 ggccccattt gctggcccct gcctgccagg cgcgcaaata atcgttgagc atatgataca
     4321 ccgttccgtc tttgcaaatc acatagtgcg cgcttacttt ggaatgttta acggtaaagg
     4381 tgtgcagggt catcggacaa ccaagctccg ccgtatggtg gataatgata aaatcgggtt
     4441 tccgcaggtc gaagttaacc gctgcggccc actccgcagt ctggcctgga aaataattgg
     4501 gtggccgttc cttcagggtg tggttgaact gcttcacctg ctttttgtag ctccggttgg
     4561 tggaacgata tggattggta cggcatccaa aaatcaggat acagagtaaa aggaaggatc
     4621 ggcgcatgca gactatttag gttgtaaaaa taatacagtt aagggctgat cgttgacgca
     4681 gaggggtttt ttatgcggat atgccatccg ctttcaagtt ccggcaggcg tcagggtctc
     4741 tccggcggat ccttccagca gctgtttcag ctgcaaggca ttgtgaatgg
//
LOCUS       DASYCU010000313         6686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3837067, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000313 DASYCU010000000
VERSION     DASYCU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3837067"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VGM24_06345"
     CDS             <1..547
                     /locus_tag="VGM24_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155024.1"
                     /translation="FFDVHAVFSNVTNISESIRKNNKMLLDFRARFLNKAGFHAELAL
                     VLNNPEGKFRLDANLGPMNATFLNPMTEPLGLAKVDRGQIQGLTYHLDADKIHGVGKL
                     TFLYSDIKVILLKKNEAQNKYKTRVLPTLAAGFLVKDSNPAKGETRVSRVDFSRDRHR
                     SIFHLMWKSMFTAVKETAGMK"
     gene            complement(571..2838)
                     /locus_tag="VGM24_06350"
     CDS             complement(571..2838)
                     /locus_tag="VGM24_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY4155025.1"
                     /translation="MVRPILRWLMFMVMAGMLFSCSETKVLPAGQYLYDGAKIKIKAK
                     PKIKKSDRKDLKEELKDLVRPNPNGSFLGIKFKLLIYNFAGKPTGKGLRYWIREKLGE
                     PPVLASYSAMEKSRAVLQNRLENRGYFRDTVLLDTIYKGRKLSAVYTAEIGYQYKIRK
                     LTYPSDPDSLSQKISRTAKRSLIKKGEPYNLDIIKNERTRIDTWLKQRGYYYFSPDDL
                     LFRADTTVGDHQVDLALLIKKSTPQQGKEVYRIGEVIVFADYDINSDTGMAVQQVPKF
                     HGYTIIDPGKKFKPEVISRALVFKSGDLYNRRNHELSLSRLISLGVFKFVKVRFDETD
                     TAKNTLNAFYYLTPAQKKSIRFEVTGLTKSDDANGGQVSVNWRNRNFFRGAELFTASA
                     YAGLERQVIGKGKSVNTNKLGLDFNLFLPRIVSPFEFRTNSGFIPKTRINLGYEYFDR
                     KDQYTLNSFKTSFGYIWKESASKEHTLDIIRINYVNPTRIDPAFQLALDTNIILARSL
                     ERQFIIGPVYNYNLNTQYRPNKKKNNFYFNGNLDLSSNLLGILSGADVAHGKEKKILN
                     TPFSQYIRAEVDFRHYHNFSKYTILASRITGGMGYAYGNSLTMPFVKQFFAGGANDLR
                     AFRSRSIGPGSYYAGNRDTAFLPDQPGDIKMEMNTELRFKLFSVIRWAFFADAGNIWT
                     LRYDSARPGSKISGHFLNQVAVGVGTGLRVDVSILLLRLDLGIPVREPFLPEHQRWVF
                     DYKNYVLNFAIGYPF"
     gene            complement(2849..>6686)
                     /locus_tag="VGM24_06355"
     CDS             complement(2849..>6686)
                     /locus_tag="VGM24_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY4155026.1"
                     /translation="LKKLSTQAVYSNKGAALKNLVIRTGRSEIRSQSAIRYLSTDAFS
                     KHPADIQTDLLFDHSRIAIPDLLLFVPALAGQLKGNEHAVLTLNGKVNGQIKNLHIAY
                     LEIGGIGNTSLAASGNIRGLPDGKKAYYDLFISRLTTSRADLFRIIPAGSLPQNIRLP
                     EQIAAGGKFTGTVKRFRVSVKATTSNGDAGVAGLLDLDRKTYDLAVSTRSADLGYILK
                     QDSLLGKISLDATAKGSGFDPKKMNSVFHVNLREGDFKSYSYKGLVLDANLHDGSGTL
                     RSSLHDPNISFELQGEGNFNGKYPSAKIKLNLDTLNLLALHLLKDTMQLHLQLDADFS
                     SSDPDALQGKLNVHDIDFSNASQTLHTDSIRLEAEHSDTAQLIQLRSEMADLDWRGKY
                     KLTQVPDAIKKLVNHYYKLPGNPDTVRVTAQDWNMALVLRPSPLLLNMIPALKGTDTV
                     RGRVYFNSEEKNLNLAVTAPKIQYNQQSVHELAFQADTKNDGISYSLNVNDAGQPGFQ
                     LHKTSLSGKIKENKLSTTLELRDKKNREQYRLSGILSQVAQGIRFAFNPDSLMLNYDL
                     WQVPAGNYVQYDSSGILVRNLNLEQHSESLRINSNGNSTQSPLNLEFSNFHIRTLTRF
                     AGQDSLMVDGTVNGKAEIKNLFSKPLFTSDLKIDNLSYNADTVGNLVLKVNNEEQNAY
                     VAHLKLNGRDNDVQVDGKYYSGESKMDLKVKLNQLNLASIKGIAASQVKDMKGYLKGE
                     LQASGNLDRPVLNGDLHFDSAVIVPAITGESLKLGGDQIEFDSDGFNFSEFVMLDSAG
                     NRATLDGNVFTSDFRKYRFDMTFNAVNFRVVNAPKEPNRIFYGKLNINAAVDVTGDQD
                     LPKVNANIRVNKNTDFTMVLPSADPEVVDRQGVVVFTDSSRKVDTAQFRHFLDSLSSH
                     ATLKGMDLSATIETDSNAQFTLVIDERNGDALAMRGRAELSGGVDKSGKTTLTGNYEL
                     DNGSYNLTLSVLHRQFIIQQGSTVTWNGRPTDADVNITAIYTVNTPSIDLVEQQLAGR
                     TTSEVNRFKQRLPFQVILHMTGKLLEPQIKFDIALPENLLALWPEVDLKLQQMRTDEA
                     EVNKQVFALLLLGRFITENPFESAAGGTNANTLARQSVSKILTDQLNQLAASLIKGVD
                     VNFDLNSNEDYTTGTLQNQTQLNVSVSKNLFNDRVRVSVGSDFELEQTNPNQNTSNLA
                     GDINLDYRLSKDGRYMIRVYRKDQYESVVEGQVIETGLSFILTFDYDQFKELFEKAKD
                     IKTPKPKKIRKQKSKK"
BASE COUNT         1529 a         1699 c         1652 g         1806 t
ORIGIN      
        1 gttcttcgac gtgcacgccg ttttcagcaa tgtgaccaat atctccgaat cgatacgaaa
       61 gaacaacaaa atgctgttgg atttccgcgc gcgctttctg aataaagccg gtttccatgc
      121 tgaactggcc ctggtgctga ataacccgga aggaaaattc aggctggatg ccaatctggg
      181 tccgatgaat gcgacttttc tcaatcccat gaccgagccc ctgggactgg ctaaagtgga
      241 cagaggccaa atccaggggc tcacctacca tctggacgct gataaaatac acggcgttgg
      301 aaaactgact ttcctgtaca gcgatatcaa ggtgatcctg ctcaaaaaaa acgaggcgca
      361 gaataaatat aagacccggg ttttgcccac tttggccgct ggctttttag tgaaagattc
      421 gaatcccgcc aaaggagaaa cccgggtttc ccgcgtggat ttttccaggg accgtcaccg
      481 gtctattttt catttgatgt ggaagtccat gttcaccgca gtaaaagaaa cggcgggtat
      541 gaaatgatcg gtcataacgg ggtgaacggg tcagaacgga tagccgatcg caaaattcaa
      601 tacatagttc ttataatcaa aaacccatcg ctggtgctcg ggaagaaagg gctcccgcac
      661 gggaataccc aggtccagcc gaagcagcag aatactaacg tccacccgca accccgttcc
      721 gacgcccacg gccacctggt taaggaaatg gccggagatt ttggatccgg gccgggcaga
      781 atcatatctc aatgtccaga tatttcccgc gtcagcaaag aatgcccacc ggatgacgga
      841 gaataattta aaccgcagtt ccgtattcat ttccatcttg atatcgcccg gctggtcggg
      901 cagaaaagcc gtatcccggt tcccggcata ataggaaccc ggcccgatgg accgcgagcg
      961 gaatgcccgg agatcattgg cgcctccggc gaaaaattgt tttacaaagg gcatcgtaag
     1021 gctattccca taggcgtatc ccattccccc cgttatccgg gaggccagga tggtatactt
     1081 cgaaaaatta tggtagtgcc ggaaatccac ttctgcccgg atgtactgcg aaaaaggcgt
     1141 gttcaatatt ttcttttcct ttccatgggc tacatcggca cccgaaagaa tgccgagcag
     1201 gttggaggaa agatccaggt tcccgttaaa ataaaaattg ttctttttct tattggggcg
     1261 gtattgggtg ttcaggttat aattatatac gggtccgatt atgaattgtc tttccaggct
     1321 ccgggccagg ataatattcg tatccagtgc gagctgaaag gcgggatcga tgcgcgtcgg
     1381 attcacataa ttgatccgga taatatcgag cgtatgctcc ttggaagcgc tttctttcca
     1441 gatatatcca aaactggttt taaaagaatt cagggtgtac tgatctttcc ggtcaaaata
     1501 ttcatagccc aggttgatgc gggttttcgg gataaaaccg ctgttggtgc ggaactcgaa
     1561 cggtgaaacg atccgcggga gaaacaggtt aaagtcaagg cccagcttat tggtattgac
     1621 actcttccct ttcccgatca cctgtctttc cagccccgcg taggcgctgg cagtgaataa
     1681 ttccgcgcca cggaaaaaat tccggttccg ccagtttacg gatacctgtc cgccgttcgc
     1741 atcatccgat ttggtaagac ctgtgacctc gaagcggatg gattttttct gcgccggggt
     1801 gagataataa aacgcattca gggtgttttt tgccgtatcg gtttcatcaa agcgcacttt
     1861 tacgaattta aatacaccca ggctgatcaa ccggctcagg gagagttcgt gattgcgccg
     1921 gttatacagg tcgccggatt taaagaccag cgcgcggctg atcacttccg gtttaaactt
     1981 tttcccggga tcgataatgg tatacccatg gaatttcgga acctgctgca cagccattcc
     2041 cgtatctgaa ttgatatcgt agtccgcaaa tacgatcacc tctccgatcc ggtatacttc
     2101 ctttccctgc tgcggagttg acttcttgat cagcagggcc aggtccacct ggtgatcccc
     2161 cacggtggta tccgcgcgga acagcaggtc atccgggctg aaataataat atccccgctg
     2221 tttcaaccag gtgtcgatcc ttgtcctttc gtttttgatt atgtcgagat tgtagggctc
     2281 gccctttttg atcaatgacc gtttggccgt tcttgaaatt ttctgactga gtgaatccgg
     2341 atcggaagga taggtcagct tccggatctt atactgatac ccgatttcag cagtgtaaac
     2401 agccgacagc ttcctgcctt tgtaaatggt gtccagcaac acggtatctc tgaaataccc
     2461 gcggttctcc agccggttct gaagcacggc ccggcttttt tccatagcgg aataggaagc
     2521 cagcaccggg ggttctccca atttttcccg gatccagtac cgtaagcctt taccagtggg
     2581 tttgcccgca aaattataga tcagcaattt gaatttgatt cccaaaaaac tgccatttgg
     2641 attggggcga accagatcct taagctcttc cttcaggtct ttccggtcag atttttttat
     2701 tttcggtttc gctttgatct ttattttggc gccgtcatac aggtattgac cagccggcag
     2761 gacttttgtc tcactgcagg aaaagagcat cccggccatc accataaaca ttaaccaccg
     2821 gagtatcggg cgaaccattt tagttttatt actttttgct tttctgtttc ctgatctttt
     2881 ttggcttcgg cgtcttgata tcttttgcct tttcgaataa ttccttgaac tgatcgtaat
     2941 caaaggtgag aatgaagctc agaccggttt cgatgacctg gccttccacc acactttcat
     3001 actggtcttt ccggtaaacc cggatcatgt atctcccatc cttactcagc cggtaatcca
     3061 ggttgatatc tcccgcgagg tttgaggtat tctgattcgg attggtctgt tccagttcaa
     3121 aatcgctgcc gacgctcacg cggactcggt cattgaacag gttcttcgaa acactcacgt
     3181 tcaactgcgt ttggttctga agggtgcccg tcgtataatc ttcattagag ttcagatcaa
     3241 aattcacatc cactcctttg atcagggaag cggccagctg attcagctga tcggtgagga
     3301 ttttgctgac actctgccgg gcaagggtat tggcattggt tcctcctgcg gcgctttcaa
     3361 aggggttttc cgtgatgaac cggcccagta acagcagcgc aaagacctgc ttgttcacct
     3421 ccgcttcatc ggtgcgcatc tgttgcagtt tcagatccac ttcgggccag agggccagca
     3481 ggttttccgg cagggcaata tcaaatttta tctgcggctc cagtaatttg ccggtcatgt
     3541 gcaggatcac ctggaagggc agccgttgct tgaagcggtt cacttcagag gtggtgcgac
     3601 cggccagctg ctgttccacc aggtcgatgg atggcgtatt gaccgtatag atcgcggtga
     3661 tgttgacatc cgcatccgtc ggacggccat tccaggtgac cgtactcccc tgctggatga
     3721 taaattgacg gtgcagcacg ctcagcgtaa gattatagga cccattgtcg agctcataat
     3781 ttccggtaag cgtggttttc ccgcttttgt ccactccccc ggacagttcc gcacgaccgc
     3841 gcatcgccag tgcatctccg ttccgttcat ctatgaccag ggtgaactgg gcattgctgt
     3901 ctgtttctat cgttgccgac aaatccattc cctttaaggt ggcgtgtgaa ctcagggaat
     3961 ccaggaaatg tctgaattgg gcagtatcca ctttccggct gctgtcggta aagaccacca
     4021 ccccctgccg gtccaccact tcgggatcgg cgctgggaag gaccatggtg aaatcggtat
     4081 tcttattcac ccggatattc gcattcactt tgggcaggtc ctggtcgccc gtaacatcca
     4141 cggccgcatt gatattcagt tttccgtaaa atatccggtt gggttctttg ggggcattca
     4201 ccacccggaa attcaccgcg ttaaacgtca tatcaaaccg gtactttcta aaatcactgg
     4261 taaatacatt cccgtcgagg gtggcccggt ttcccgcgga atcgagcatg acgaattcgg
     4321 aaaaattgaa cccgtcactg tcgaattcga tctggtcgcc acccagcttt aatgattctc
     4381 cggttattgc cggcacaatc accgcactgt caaaatgaag atcgccgttt aataccggcc
     4441 ggtccaggtt tccgctggcc tgcagttctc ctttgagata acccttcata tcttttacct
     4501 gggatgcggc aatgccttta atggaagcca ggttcagctg attcagtttc accttcaggt
     4561 ccattttgct ttccccggaa taatattttc catccacctg cacgtcattg tccctcccat
     4621 tcagtttcaa atgggcgaca tacgcgttct gctcttcatt attcactttc aaaaccaggt
     4681 tacctacggt gtcggcatta taagacaggt tatcgatctt taagtcggac gtgaacaggg
     4741 gttttgaaaa caggttcttt atttccgcct tgccgttaac ggtcccgtcc accatcagcg
     4801 aatcctgtcc ggcaaaccgg gtgagcgtgc gaatgtgaaa gttggaaaat tccaggttca
     4861 gcggcgattg ggtgctgttt ccgttactgt tgatcctgag cgattcggaa tgctgttcca
     4921 ggttaagatt gcggaccagg atgccggagg aatcgtactg aacataatta ccggcgggaa
     4981 cctgccacag gtcataatta agcatcaggc tatccgggtt aaaggcaaaa cggatccctt
     5041 gcgccacctg ggacaaaatt ccggaaagcc ggtattgttc cctgttcttt ttgtctctca
     5101 gttccagggt ggtggatagt ttattttctt ttatttttcc ggaaagggaa gttttatgca
     5161 actggaaccc cggctggcca gcatcattca cattcaatga ataactgatc ccgtcgtttt
     5221 ttgtatccgc ctggaaggcg agctcatgaa cagattgctg attgtactgg atcttcggtg
     5281 cggttaccgc caggtttaaa ttcttttctt cgctgttgaa ataaaccctt ccccgcacgg
     5341 tatccgttcc ttttaacgcg ggaatcatat tcaacagcaa aggagacggg cgcaggacga
     5401 gggccatatt ccagtcctgt gcagtcaccc ggaccgtatc cggatttccg ggtagcttat
     5461 aatagtgatt gaccagtttc tttattgcat ccggtacctg ggtcaattta tattttcctc
     5521 tccagtccag gtcggccatt tccgaacgaa gctgaatcaa ctgggctgta tccgaatgtt
     5581 cggcttccag gcggatacta tccgtatgca gggtttgact ggcatttgaa aaatcaatat
     5641 catgtacatt gagcttaccc tgtaaggcat cgggatcgct ggagctgaaa tcagcgtcga
     5701 gctggagatg gagctgcatg gtatctttca ataaatgcag ggccagcagg ttcagggtat
     5761 ccaggttcag ttttattttt gccgaaggat attttccatt aaaattcccc tctccctgca
     5821 actcaaagga tatattcgga tcatgcaggg aagaacgcag ggttcccgag ccgtcatgca
     5881 ggttggcgtc cagcaccagg cctttataag aataggattt aaaatctcct tcccggaggt
     5941 tcacgtggaa gacgctgttc atctttttcg gatcgaatcc agagcccttt gccgtcgcat
     6001 ccaggctgat ctttccgagc aggctatcct gttttaagat ataaccgagg tcagcagacc
     6061 gggtgctgac tgccaggtca tacgttttcc gatccaggtc gagcagtccg gccaccccgg
     6121 catctccatt gctggtggtc gccttcacgc tcacccgaaa gcgtttaacc gttcccgtga
     6181 attttccgcc ggcagcgatc tgctccggga ggcggatatt ctgcggaagg gaaccggccg
     6241 gaatgatccg gaacaggtcc gccctcgaag tggtcagccg cgatatgaac aggtcataat
     6301 aggctttctt tccatccggc aatccccgga tatttccgga agcggccagt gaggtatttc
     6361 caattccgcc aatctccagg taagcgatat gaaggttttt tatttgtccg ttcacttttc
     6421 cattcagcgt cagcacggca tgctcatttc ctttcagttg tccggccagc gccggaacaa
     6481 acaggagcag atcgggaatg gcgatccgcg aatgatcaaa caggaggtcc gtttgaatat
     6541 ctgcgggatg tttgctgaat gcatctgttg aaagataacg gatggcggac tggctgcgga
     6601 tttccgaacg gccggtccgg ataacgaggt ttttcagcgc tgcccctttg ttgctgtaca
     6661 cggcctgcgt ggagagtttt tttaaa
//
LOCUS       DASYCU010000314         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2532520, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000314 DASYCU010000000
VERSION     DASYCU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532520"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VGM24_06360"
     CDS             <1..450
                     /locus_tag="VGM24_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY4155027.1"
                     /translation="WAFAGFSLGGLSALDISWAHPHIFRNTGVFSGSLWWRSLDKADP
                     GYSDEVHRLMHNLIHNGAYVPGVKFFFECGTEDEEEDRNHNGVIDSIDDTKDLIRELV
                     QKGYSEKEDIYYLEIPGGTHDPATWASAMPHYLKWAWGPAASKDQKL"
     gene            complement(438..>2057)
                     /locus_tag="VGM24_06365"
     CDS             complement(438..>2057)
                     /locus_tag="VGM24_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155028.1"
                     /translation="DASGVASVYASQYQTVYSTDPKNPKPVANITASQLGFYAQDAFS
                     VRDNFNITYGLRADIPLFTKKPVSNEAFNSSDIAVSNGVATNKVPKNSILLSPRIGFN
                     WDVKGNRQTQVRGGVGIFTGRVPFVWISNQYSNTGIGTISSTLNATQVASNDVHFNPN
                     APFHPSTGVGPGINVTDPDFKYARTLRSNLAIDQRLPYGVVATLEGIYTRTIQDILYH
                     DLNLAPASTSLILGNTTRPFYGLKNNTGYSSIYALSNTTQGHSYNLTLSLARLFNRGW
                     SANISYSLGHSWGVIDGTSSTAASNYRYNYNINGLNNIEEARNNYDQGSRVVGYIGKK
                     FSYGKIFSTNIGLVYTGQSGQPFSYVYFGDINGDDGSTPAKLSTAGGADLIYMPGDAS
                     QFVDKNGLTADQEFAAFQTYMNSTKYLKSHIGKNTERNGDRLPWENHFDLKVEESIAV
                     YKQHVLSVSVNIFNISNLLSNKWGHSYYLGNQEAQPLDVDHFTTNGDGSITPYYYFNP
                     TFGLNKYTNKPWAYNDFLSRWNLQLGLRYSF"
BASE COUNT          500 a          477 c          556 g          524 t
ORIGIN      
        1 tgggcttttg ccggcttttc cctgggtgga ctgagcgctc tggatatcag ctgggctcac
       61 ccgcatattt tccggaacac cggcgttttt tccggatccc tctggtggag aagcctggac
      121 aaagcggatc ccggatacag cgatgaggtc catcgcctca tgcataacct gatccataac
      181 ggcgcctacg ttccgggagt gaaatttttt tttgaatgcg gaacggaaga tgaggaggag
      241 gaccgcaatc acaatggcgt gatcgattcc attgacgaca ccaaagacct gatccgggaa
      301 ctcgttcaaa agggttactc tgaaaaagag gatatatatt acctcgaaat accgggcggt
      361 acgcacgacc cggccacctg ggcatccgcc atgccccatt atcttaaatg ggcctggggc
      421 ccggccgctt ccaaagatca gaagctgtaa cggagtccca attgcaggtt ccagcgcgaa
      481 aggaaatcgt tgtacgccca gggtttattg gtatacttgt ttaaaccaaa agtgggattg
      541 aaataataat acggggtaat gctaccgtcg ccgttggtgg tgaaatgatc cacatccagg
      601 ggctgggctt cctggtttcc gagataatag gaatgacccc atttattgct cagcaggttg
      661 ctgatattga aaatattaac cgagacggac agcacgtgct gtttgtaaac cgcaatggat
      721 tcttccactt tcagatcgaa atgattttcc cagggcagac ggtctccatt cctttccgtg
      781 ttcttaccga tatgactctt caggtatttt gttgaattca tatacgtctg gaacgcggca
      841 aattcctggt ctgccgtcaa gccgttcttg tcgacaaact gggaagcatc cccgggcatg
      901 taaatcaggt cagcgccacc ggctgtactg agttttgcgg gagtggaacc atcatccccg
      961 ttgatatctc caaaataaac gtaggaaaaa ggctggccgg actgtccggt atagaccagg
     1021 ccgatattcg tggagaatat ctttccgtaa ctgaactttt tgccgatata cccgaccacc
     1081 cgggaaccct ggtcataatt gttcctggct tcctcaatat tattcagtcc attgatatta
     1141 tagttgtaac ggtaattgga agcggcggtg gaggatgttc catcgatcac tccccaggaa
     1201 tggccgaggc tgtaggaaat atttgcgctc catcccctgt tgaaaaggcg cgcaagagaa
     1261 agcgtcagat tataagaatg cccctgggtg gtattgctga gtgcgtaaat gctggagtat
     1321 ccggtgttat tctttaggcc atagaagggg cgggtggtat tgcccagaat aaggctggtg
     1381 gaagcaggag ccaggttgag atcatgatat agaatatcct gtatggtcct ggtataaatg
     1441 ccctcgagcg tagctacgac tccgtaaggc aaacgctggt cgatggccag attgcttctg
     1501 agcgttctgg catatttaaa atcaggatcc gtcacattga ttccgggccc cacgcctgta
     1561 ctgggatgaa agggcgcatt cggattaaaa tgaacatcgt tgctggcaac ctgtgtcgcg
     1621 ttgagtgtgc tggaaatggt tccgatgccg gtgttgctgt actggttgga gatccagaca
     1681 aacggtacgc ggccggtaaa gatcccgacg cccccccgga cctgggtctg acggtttcct
     1741 tttacatccc agttaaatcc gatgcgggga gaaagcagga ttgaattttt tggcactttg
     1801 ttggttgcca ccccgttgct tactgcgata tctgaactgt tgaatgcttc gttggaaacc
     1861 ggctttttgg tgaaaagcgg aatatctgcc cggaggccgt aagtaatatt gaaattatcc
     1921 cggaccgaga aggcatcctg tgcataaaaa ccaagctggg aagccgtgat attggcaacc
     1981 ggttttggat ttttcggatc ggtggaataa acggtttgat actggctggc atatacagac
     2041 gcgacgccgg aggcatc
//
LOCUS       DASYCU010000315         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2968039, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000315 DASYCU010000000
VERSION     DASYCU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2968039"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1133)
                     /locus_tag="VGM24_06370"
     CDS             complement(75..1133)
                     /locus_tag="VGM24_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEY4155029.1"
                     /translation="MMKRTIRNAGLFLFGLMILFSLAGMAQFGEPVKLVKADHGNRID
                     ILVGGKLFTRFLYTDSLEKPVLYPLCSSKGTVVTRGFPLDPRPGEPTDHPHHLGCWFT
                     FENVNGLDFWNNSYAIPADKKHLYGWIRTDRIEKIKDGKPGTLIYHANWTNQQGKVLL
                     EETTRFEFSGNEHQRMIDRITELKADTTVLFADAKDGMLGLRLAHELQIPAKEDQTFT
                     DNKGNKTLVKGGTDKIANGDYLTSEGKTGDSAWGTRGVWCTVFGKMGNDTVSISIIDH
                     PQNPGYPTYWHARGYGLFAANPLGQKIFSNGKREMNLRLKKGQTVRFRYRILIEENSR
                     STPVAELNRLAADFARTP"
     gene            complement(1152..>2472)
                     /locus_tag="VGM24_06375"
     CDS             complement(1152..>2472)
                     /locus_tag="VGM24_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155030.1"
                     /translation="SLAGGAVLVSKMGWTASSYRRIIGANDRVRVGVVGFSDRHKATH
                     MPCFMNHYKELNFDVVAVSDIWNRRREEGAAKWKEKMQHDIIACRNNDELYGKKLVDA
                     VFVSTADFQHARHAIEAVNAGCDAYVEKPFAETMEDNRAALKAIKASGKIVQIGSQRR
                     SGANYMSANEFIRSGKFGPIKMVELTWNVNQPGRWRRPELTGLIKKEDTDWERFLLNR
                     PKDEWDPRKYLEFRLFWPYSSGLPAQWMSHQIDTVHWFTGLKHPRSVVANGGIYMWKD
                     GRKNWDTITAAFDYGPADDPTNGFQVVFASRMDNGVENPSEIYYSNGGELNLNTNKVS
                     PAGGLTKRFADAMHLEPNLLPEMTLSTAEKVVTSANTGGDALTSAHIRNWMECIRSRK
                     EPHAPVEAGYSHSIANIMTNAAVHTGGKATFDENSQEVMADGKVFRY"
BASE COUNT          519 a          702 c          582 g          669 t
ORIGIN      
        1 acctacctgg gaccgcccga aacgctaatt cttccctgaa cattagctcc cattcgcgtc
       61 agcagcctgc cgcattaggg agtccgggca aaatccgctg cgagccggtt cagttcggcg
      121 accggcgtgg atcttgagtt ctcctcgatg agaatgcgat agcggaaacg gaccgtttgc
      181 ccttttttta aacgcagatt catttccctt tttccgttac tgaatatttt ctgacccagc
      241 ggattggcgg caaacaaacc ataaccgcgg gcatgccagt aggtgggata accgggattt
      301 tgcggatggt cgataatgct gatgctgacg gtatcatttc ccattttccc aaatacggta
      361 caccatactc cccgggttcc ccaggcggaa tcaccggttt taccttcact ggttaaatag
      421 tctccattgg caatcttatc cgttccgccc tttacaaggg ttttgttccc tttattgtct
      481 gtaaaggtct ggtcttcttt tgccgggatc tgcagttcat gtgccagacg caaccccagc
      541 atgccgtctt ttgcatccgc aaagagaact gttgtatctg ccttcaattc ggtaatccgg
      601 tcaatcatcc gctgatgttc gttcccgctg aattcaaaac gggtggtttc ctccagcagt
      661 acttttccct gctggttggt ccagttggca tgatagatca gggtgccggg cttgccatct
      721 tttatttttt cgatccggtc tgttcttatc catccgtata aatgtttttt atccgccgga
      781 atggcgtagg aattattcca gaaatccagt ccgttcacat tttcaaaagt aaaccagcat
      841 cccaggtggt gcggatgatc ggtgggttct cccgggcggg gatccaaagg aaatccccgg
      901 gtgaccaccg ttcccttcga cgagcacagg ggatataata ccggcttttc caggctgtcc
      961 gtatataaaa accgggtaaa taactttcct ccgaccagga tatcaatgcg gtttccatgg
     1021 tctgctttca ccagcttcac cggttcgcca aactgggcca ttccggccag tgaaaataaa
     1081 atcatcaggc cgaataaaaa taaaccggca ttccggattg ttcttttcat cataaaaata
     1141 ttttctgcca tttaatatct gaagactttt ccatctgcca tcacttcctg cgaattttcg
     1201 tcgaaggtcg cctttccacc ggtatgaacc gccgcgttgg tcatgatatt ggctatggaa
     1261 tggctgtatc cggcttccac cggagcatga ggttccttcc ggctccggat gcattccatc
     1321 cagttgcgga tatgcgccga cgttagtgca tcccctcccg tgttggccga agtgaccact
     1381 ttttctgcag tggacaacgt catttccggc aggaggttcg gctccaggtg catggcatcg
     1441 gcaaatcgtt tggtgagccc gccggccggg gaaaccttat tcgtgttcag gttcagttcc
     1501 ccgccgttgg aataataaat ttcagaaggg ttttcgaccc cattgtccat gcgggaggca
     1561 aaaacaacct ggaagccgtt cgttgggtca tccgccggac cgtaatcaaa agcggccgta
     1621 atcgtatccc agttctttct tccgtccttc cacatgtaaa tgccgccatt ggccaccacg
     1681 ctgcggggat gtttcaatcc ggtaaaccag tgcaccgtat cgatctggtg gctcatccat
     1741 tgtgcgggca agccggagga atacggccag aacagccgga actccaggta ctttctggga
     1801 tcccattcat ctttcggccg gttcagcaga aatctttccc agtcggtatc ttcctttttt
     1861 atcagcccgg tgagttccgg cctgcgccag cggcccggct ggttcacatt ccaggtaagt
     1921 tccaccatct tgatcggccc gaattttccg gaacggataa attcgttggc ggacatataa
     1981 ttcgcgccgc tccttctctg cgagccgatc tgcacaattt ttccgctggc cttaatggct
     2041 ttcagcgccg cccggttatc ttccatggtt tccgcaaagg gtttctccac atacgcatcg
     2101 caacccgcat tcaccgcttc aatggcatga cgggcatgct ggaaatccgc cgtgcttaca
     2161 aatacggcat ccaccagctt ttttccgtag agttcatcgt tgttgcggca ggcgataata
     2221 tcatgctgca ttttttcctt ccactttgcg gctccttcct cccgtctccg gttccagata
     2281 tccgacacgg ccaccacatc aaaattcaat tccttataat gattcatgaa acagggcata
     2341 tgcgttgcct tatgccggtc ggaaaaaccg accaccccca cccgcacgcg gtcgttcgca
     2401 ccgatgatcc gccggtagct gctggccgtc cagcccattt ttgaaacgag tacggcgccg
     2461 cccgcaaggg aa
//
LOCUS       DASYCU010000316         8587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4994239, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000316 DASYCU010000000
VERSION     DASYCU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8587
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4994239"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGM24_06380"
     CDS             complement(<1..188)
                     /locus_tag="VGM24_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155031.1"
                     /translation="MKPTYKSVSLIDSKSFKVIRQDNKQEFDFPWHFHPEFELTYISN
                     SQGIRYVGNNVENFYEDDL"
     gene            complement(322..1260)
                     /locus_tag="VGM24_06385"
     CDS             complement(322..1260)
                     /locus_tag="VGM24_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155032.1"
                     /translation="MKLEEKVNYMGWPNCLRLSNSEIEVIIATDIGLRILRFGFIDGQ
                     NILYLSPEDKGKQGGDGWRIFGGHRLWHSPEAIPRSYSPDNDPISYRYDQQTLKITQP
                     VEETTGIVKEMEISLSPDLNQLRILHRLINMNLWDVKLSAWALSALAENGLAIIPQEP
                     FGEGDAYLLPARSLALWSYARMEDPRWSWGNKYIRARQDPSRSSEQKIGLMNKQGWAA
                     YCLNEDLLIKVFDFDPVAEYPDFGSNQEIYINGRFLEMETLGPLVNVPPKGKTEQVEH
                     WLLSRAKTGETDESVERELIPRLSCFQDRLSLIRKK"
     gene            complement(1271..2398)
                     /locus_tag="VGM24_06390"
     CDS             complement(1271..2398)
                     /locus_tag="VGM24_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY4155033.1"
                     /translation="MEQTVSHIGKLKQIKNASRTDAFLITSPSSVKYFSGYFFYFEYG
                     PSPFHLLPAILMVVPDQDSGLVLADNEMGQASPVDPAIRLIPYESYTFETAPDPVAAC
                     IKKLCEFIDRNRLSGARIGIEQATLPFAVVKCLQGLYPSLEWVDVSPEIGQCKLIKDA
                     GEIALLRRASALADTGQQAVLKYARAGMTELELFSLAHRDMEAAAGYRIPLMSDLSSG
                     KGTNSGGGMPTDKIIEAGDLILSDFQPCLQGYWGDSCNTMAVGQATAEQKNTFIRVRE
                     ALEIGIGAIKPGVKAKTVDRLMRDHIGNYPHHSGHSVGTAYHESPRITPYNNMELMPG
                     MLVALEPAIYKEEYGIRLEHLLLVTAGGCEVLTQFRHRFEQ"
     gene            complement(2404..3522)
                     /locus_tag="VGM24_06395"
     CDS             complement(2404..3522)
                     /locus_tag="VGM24_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEY4155034.1"
                     /translation="MKITNVQAIVLKLPEISSAADGTQDDLIIVVETDEGITGYGEVD
                     TAPGVGKAVVDAYMSHGTCYGLREVVKGSDPFDYEQIWNDMWAKTYYYGRSGPVMHVM
                     SGIDMAIWDIMGKATGKPVHKLLGGSYTDKVRAYASALMPENKEEVKRKVESYISQGF
                     TAIKFGWGPLGYDVHFDIELVKAARKAAGDQVDILIDIGKRYRLKSAFYVAKALEQLN
                     IYWLEEPLPAEDYIGYKRLTESTSLRIATGEEESGRLAFARLINETHVDVIQPDMSRC
                     GGLTEAKKIATMTADQNMLCVPHAFKTGVLVAASIQLIAAIPNAPFLEFSVTDSAIRK
                     ELLVNPFKQKDGFVDVPQTPGLGIELNPDVIKKYGIAK"
     gene            complement(3519..4475)
                     /locus_tag="VGM24_06400"
     CDS             complement(3519..4475)
                     /locus_tag="VGM24_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4155035.1"
                     /translation="MNSIKSQKIVDWSLVIIVNFMWATQVPVIRLIGDAYGPITVAFV
                     PMILSTVIFLPALWMENKRRNVKMKWRWRDIRYFVLPGLIGVFLMQYLYTLGSRMTLA
                     ANAGIITLTIPVIVAVFASFLLKEKLNIVRILGFVLAIGGVLMTSLPDISGANFKHGS
                     YLAGNLIFLFACCCCGFYNTYCKLLVDKKFTELEILVYGSIIGSIVSIPLLIWVEPFH
                     YSTFIHSGKLALFGVLELSLIVYGVSMLLFFYILKRMDVTQAILGNYLLPFFIALLGI
                     LLLDEKITVLMFTGGILILLSTLMVTIYENTLLRMIKKNKTE"
     gene            complement(4495..5175)
                     /locus_tag="VGM24_06405"
     CDS             complement(4495..5175)
                     /locus_tag="VGM24_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEY4155036.1"
                     /translation="MKSFKNDAELFLFVRKHLYVAAVCDILDEMGYRNQAMHQRLRPL
                     LTDVRNCGFVGRARTFHWVETDTIVESDPYGLEIEAMDSLKAGDVVVHSTDYNGTNAP
                     WGELMSTISKNKGVVGCVCDSQVRDCIKIMEMRFPVFYAGIRPLDSKGRGMVTGYDVA
                     VECGGVLVNPGDLIYSDFDGIVVVPQGIEDEVFQRAKEKVENENHSRRELLDGRSLRQ
                     VYDKYKSL"
     gene            complement(5249..6568)
                     /locus_tag="VGM24_06410"
     CDS             complement(5249..6568)
                     /locus_tag="VGM24_06410"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY4155037.1"
                     /translation="MGKGFLLLCGFLSLLFSSSVSVAQTNSPERFTRPEPSDWYTGDI
                     HVHRSCDGSKPVPSSDLPAMMKVNHLAVISVLGDMGNNNSADRLEDLHKVDGSDSPLS
                     DSDQIIHYAAEWHWDATQWQFPHQALGGHLVLLGLREAHKIWDPSAYKVLEWAGQQHA
                     VRGFAHMQYLNNRIQDTLDCCIPIDYPVEAALKNIEFVAEEQPRGSENGILAYYRLLN
                     CGFRLSLAGGTDYPCNRVALGSVLTYVEVPGKITYQKWIEGIAKGRTVVSRNAHNEFL
                     DLKVNKTEGPGKVIRIKKGGKVNIGVSWSAGKELSGQVELVSNGRVIARQKGMARPGD
                     PLVLHTTQTFSKSGWICARRMDSLGRTHFLHSSPVYVIVDDQPIRASTEDAEYFVAWI
                     DNILQRIEPGGPWNKYFPDDLETVKAHYRKARDIYKKIVAESRHLNK"
     gene            complement(6625..8205)
                     /locus_tag="VGM24_06415"
     CDS             complement(6625..8205)
                     /locus_tag="VGM24_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155038.1"
                     /translation="MKNKSILIYSLLALFASSCKKGFLDRQPLDQYSETSLWTSANDA
                     SAALTGCYSGFEDGTWIFYMPCASDNAFSAFPGEGYEEYGNMVLLTPNHTPNNKWNFN
                     SIQKCNWFLANIDKTPMDDGLKKRMKGEARFLRAYKYFIMSQLYGDVPLITANLTTQE
                     ADTVSRTPKDQVVQFILDELGSIAADLPDSYTGADVGRITSGAAWALKARTELYNQKY
                     ADCISSCNNIMGKYSLFPNYTDLFRIQNEHNQEIILDIEYIENDAPLTSLGRNALHFQ
                     GGGGGGWYSIDPSQSLVDAYEMSNGKTIDDPSSGYDPDQPYQNRDPRLAATVVVPGSL
                     VDGVYFNPLQPGTLDYWLRHNFTGYASRKYVSHLSDFDDMWNVGLNIPLIRYAEVLLT
                     YAEAKIELNQIDGTVYAAINQVRNRAGMPDVDQTLYNSQTKMRELIRRERRVELAMEG
                     LRWFDIQRWKIGEQVMSGPLYGSRLGTVDPNNGHLTLSTDRILSDQRTFDPSKNYLWP
                     VPQPEIDINKNITQNPGY"
     gene            complement(8229..>8587)
                     /locus_tag="VGM24_06420"
     CDS             complement(8229..>8587)
                     /locus_tag="VGM24_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4155039.1"
                     /translation="TGKPTSIFSDHWTPDNPNAKFPRLWNSYTQNDPDYNNSSFWVRN
                     AGYMRLKNLQVGYTLSSGWLARVGIQKARIYYSGQNIFTSTRFYNWIDPEAPAGESGN
                     TYPQVLINTVGLNITF"
BASE COUNT         2137 a         2270 c         1972 g         2208 t
ORIGIN      
        1 aggtcgtctt cgtagaaatt ttcaacgtta ttcccaacat accggattcc ctggctattg
       61 gaaatatagg taagttcaaa ttctggatga aagtgccagg ggaaatcaaa ctcctgttta
      121 ttgtcttgcc ggattacctt aaaagatttt gagtctatca gggaaaccga cttgtaagta
      181 ggtttcatat ctattatttt gtaccgcctg tatcaaaatt gaccaagtaa tatacaaaat
      241 aaggtaatga aataggaaaa ataaagggtt ttaatttatt tccatcttcc aaaactgaat
      301 ccatggacga tcaaacacat cttatttttt tcttataagg ctcagccggt cctgaaagca
      361 ggataaacgg ggtataagtt cccgttcaac ggattcgtcg gtctctccgg tttttgccct
      421 ggaaagcagc cagtgttcca cctgctcggt tttccctttc ggaggaacat tcacgagcgg
      481 gcccagggtt tccatttcca ggaaccggcc gttgatatat atctcctggt tacttccaaa
      541 atccgggtat tccgctacgg ggtcaaagtc aaataccttg atgaggaggt cttcattcaa
      601 gcaataagcc gcccatccct gcttattcat caggccgatt ttttgttcgg acgagcggga
      661 aggatcctgt cttgcccgga tatatttatt tccccaggac cagcggggat cttccatgcg
      721 ggcatagctc cagagtgcca gggagcgggc gggcagtaaa taggcgtccc cttctccgaa
      781 cggctcctgg gggatgatcg ccaggccgtt ttcagcaagg gcggataatg cccaggcgga
      841 taatttaacg tcccagagat tcatattgat caggcggtgg agaatcctaa gctggttcag
      901 atccggcgaa agactgatct ccatttcctt tacgatgccg gtagtttctt ccaccggttg
      961 ggtgatcttc agcgtttgct gatcataccg gtaactgatc ggatcattgt cgggagaata
     1021 agatctcgga atagcttccg gtgaatgcca caaacggtga cctccgaata tccgccatcc
     1081 atcccctccc tgttttcctt tgtcttccgg tgacaaataa agaatattct gcccatcaat
     1141 gaagccaaat cgaagaatac gcagaccaat atccgtagca atgatcactt ctatttcgga
     1201 attgctcaac cgaagacaat tcggccagcc catatagtta actttctcct ccagtttcat
     1261 attagctgaa ttattgttca aaacgatgcc ggaactgggt gagcacttcg caacctcccg
     1321 ccgtaaccag caacaggtgc tccagccgga tcccgtattc ttccttataa atagccggct
     1381 ccagggcaac cagcattccc ggcatcagtt ccatgttatt gtaaggggtg atgcggggag
     1441 attcatgata ggccgttccc acgctgtggc cgctgtgatg agggtaattt ccaatatggt
     1501 cacgcatcag ccggtcgacg gttttcgcct taacacccgg tttgatggct ccaatcccga
     1561 tttccaatgc ttccctaacc cggataaagg tattcttttg ctctgccgta gcctggccaa
     1621 cagccatcgt attgcaggaa tcaccccagt atccctggag acagggctgg aagtcggaca
     1681 ggatcaggtc gcccgcttca attattttat ctgtcggcat cccgcctccg ctgttggtcc
     1741 cttttccgct gctcaggtcc gacatcagcg ggatccggta tccggcagcg gcttccatat
     1801 cccggtgggc cagcgaaaaa agttccagtt cggtcatccc tgcccgagca tattttaaaa
     1861 cggcctgctg accggtatcc gcaagggcag aagcccggcg aaggagagcg atctccccgg
     1921 catccttgat caatttacat tgaccgatct ccgggctcac atccacccat tccagagatg
     1981 gataaagccc ctgcaggcat ttcaccacgg caaacggaag cgttgcctgt tcaatgccaa
     2041 tccgcgcacc ggagagccgg ttccggtcta taaattcgca gagttttttt atgcaggctg
     2101 cgaccggatc gggagcagtc tcaaaggtat aactctcgta agggatcagc cggatggccg
     2161 gatccaccgg tgaagcctgg cccatttcat tatccgccag caccagaccg gaatcctgat
     2221 ccggaaccac cattaatatg gccggaagca gatgaaaagg ggaaggcccg tattcaaaat
     2281 agaaaaaata gccgctgaaa tattttacag acgagggaga tgtgatcaga aaggcatccg
     2341 tccggcttgc attcttaatc tgtttcaact tcccaatgtg tgacaccgtc tgctccatga
     2401 aactcatttt gctattccat attttttgat aacgtccgga ttcagttcaa tgcccagtcc
     2461 gggagtctgg ggcacgtcta caaatccatc tttctgttta aacgggttta cgagcaactc
     2521 cttccggata gcggaatccg taacggaaaa ttcaagaaaa ggagcgttcg gtatggccgc
     2581 gatcagttga atggaagccg ccaccagtac accggtttta aacgcatgag gtacacaaag
     2641 catattctgg tcggccgtca tcgtggcgat cttttttgct tccgtcaatc cgccgcagcg
     2701 cgacatatcg ggctggatca cgtccacatg tgtttcgttg atcagcctgg caaaagccag
     2761 ccgcccgctt tcctcctctc ccgtcgcaat ccggagcgag gtggattccg taagccgttt
     2821 gtatccgata taatcctccg cgggcaaggg ttcttccagc cagtaaatat tcaattgctc
     2881 caaggctttt gccacataaa aagcggattt cagccggtat cttttgccga tgtcaatcag
     2941 gatatccacc tggtcaccgg ctgccttccg ggccgctttg accaattcaa tatcaaaatg
     3001 cacatcgtaa ccaagcggtc cccagccgaa tttgatggcc gtgaatccct gtgaaatata
     3061 ggattccacc tttcttttga cttcttcctt attttcaggc attaaagcgc tggcataagc
     3121 ccggacctta tccgtataac ttcctcccaa taacttatgg acgggcttgc cggttgcctt
     3181 gcccatgata tcccagatcg ccatatcaat gccgctcatc acatgcatga cggggccgct
     3241 tcttccataa taatatgttt tggcccacat atcattccag atctgttcgt aatcaaacgg
     3301 atcggatccc tttaccactt cccgtaaccc gtaacaggtt ccatgtgaca tatacgcatc
     3361 cactaccgcc ttgcccactc cgggggcggt atctacttcc ccataaccgg taatcccctc
     3421 atctgtttca accacaatga tcaggtcgtc ctgcgttccg tcggcagcgc tggagatttc
     3481 gggtagcttt aatacaatgg cttgtacatt cgtaattttc attccgtctt attttttttt
     3541 atcattctca ataaagtatt ttcataaatg gtgaccatca gggtgctgag taaaataaga
     3601 atgccccccg taaacataag gacggtgatc ttttcatcca gcagcaggat gccgagaagg
     3661 gcaataaaaa agggaagcag gtaattcccc aggatagcct gcgttacatc catccttttc
     3721 aggatataaa aaaacagcag catcgacacc ccgtaaacaa tgaggcttaa ttccaaaacg
     3781 ccgaataagg ccagcttgcc gctatggata aacgtagaat aatggaaagg ttcgacccag
     3841 atcagcaggg gaatgctgac aatgctgccg atgatggaac cgtataccag gatttcgagc
     3901 tctgtgaatt tcttatccac cagcaatttg cagtaggtat tataaaagcc gcagcagcaa
     3961 caggcaaaga gaaagatcag gttgccggcc aggtagctgc cgtgtttaaa attcgcaccg
     4021 gaaatatccg ggagtgaggt catcagaacg cctccgatgg ccagtacaaa accaagtatc
     4081 cggacaatat ttaatttttc cttcaggaga aaggaagcga aaacagcaac aataacgggt
     4141 atggtcaggg taatgatccc ggcattggcg gccagggtca tccgggaacc aagggtatat
     4201 aaatactgca tcaggaaaac accgatcaga cccggcagca caaaataacg gatatcccgc
     4261 caccgccatt tcattttcac gttcctccgt ttgttttcca tccataacgc gggtaaaaaa
     4321 ataacggtgc tcaggatcat gggaacgaat gccacggtaa tcggaccata ggcatccccg
     4381 atcagccgga tcaccggcac ctgggtagcc cacataaaat taacaatgat gaccagggac
     4441 cagtctacga ttttctgaga tttgattgaa ttcatatttt tcgcggttct ccctctagag
     4501 ggatttgtat ttatcataga cctgccgcag cgagcggccg tccagcagtt cccggcggga
     4561 atggttttca ttttccacct tctcctttgc ccgctgaaac acttcgtctt ctattccctg
     4621 gggaaccacc acgatcccgt caaaatcgga atagatcagg tctcccgggt tcaccagcac
     4681 tcccccgcat tccaccgcca cgtcatagcc cgtcaccatg ccccgcccct ttgaatccag
     4741 gggcctgatc ccggcataaa acaccgggaa acgcatctcc atgatcttta tgcaatcccg
     4801 cacctggctg tcgcacacgc aacccaccac ccctttgttc ttggagatcg tggacatcag
     4861 ttctccccag ggcgcattgg ttccgttata atccgttgag tggaccacca catccccggc
     4921 cttcagcgaa tccatggcct ctatttccag cccgtaggga tcggactcca caatcgtatc
     4981 ggtctccacc cagtgaaaag tccgggcccg acccacgaat ccgcaattcc tgacatccgt
     5041 cagcaggggg cgcaggcgct gatgcatcgc ctggttccgg taccccatct cgtccagaat
     5101 gtcgcatact gctgcaacat acagatgttt gcgaacgaaa agaaacagtt cagcatcgtt
     5161 tttaaatgat ttcaccaaga tgaatttatg gttaagcagt taaagccctt acccggactc
     5221 agggaaaggc cattgaaaaa atgattattt atttatttaa atgccgggat tccgcaacaa
     5281 tctttttata aatatccctg gctttccggt aatgcgcctt tacggtttcc agatcatctg
     5341 gaaaatattt attccacggg ccgcccggtt caatccgctg taagatatta tcgatccagg
     5401 ccacgaaata ttccgcatct tccgtactcg cccggatcgg ctgatcatct acaattacat
     5461 atacggggga agaatgtaaa aaatgagtcc ggcccaggga atccattctc cgggcacaga
     5521 tccatccgct tttggagaag gtttgtgtcg tgtgaagaac caggggatcg cccggtctcg
     5581 ccatcccctt ttgcctggcg atcacccggc cattgctgac cagttcaacc tgaccggata
     5641 gttctttgcc ggcagaccag ctgaccccga tattcacttt tcctcctttc tttatacgga
     5701 taacctttcc tggtccttct gttttgttta ctttcagatc caggaattca ttatgggcat
     5761 tccgggacac taccgtacgg cccttcgcaa ttccttcaat ccacttctga taagttatct
     5821 tccccggcac ttcaacatag gtgagtacac tgcccagcgc cacccggtta cagggataat
     5881 ccgtaccacc ggccaggctg agcctgaatc cgcagttcag caggcggtaa taggccagga
     5941 tgccattttc gcttccgcgc ggctgttcct ctgcgacgaa ttctatattc ttcagtgccg
     6001 cttcaaccgg ataatctata ggtatgcagc aatcgagcgt atcctggatc cggttgttca
     6061 ggtattgcat atgcgcaaaa ccccggacgg catgctgttg cccggcccat tccagcactt
     6121 tatacgcgga cgggtcccat attttatgtg cttcccggag tccaagcaaa accagatgac
     6181 ctcccagggc ctgatgcgga aattgccatt gggtggcatc ccagtgccat tccgcagcat
     6241 agtgaatgat ctggtccgaa tcagagaggg gagaatcgga accatccact ttgtgcaggt
     6301 cctctagccg gtctgccgaa ttattattgc ccatatcacc gaggaccgag atcacggcca
     6361 gatgattaac cttcatcata gccggcaagt cgctggaggg aacaggcttg cttccgtcac
     6421 agctccggtg aacatgtata tctccggtat accaatccga aggctcgggc ctggtaaacc
     6481 gttccggaga attggtctgg gcaacggaaa cggaagagga aaaaagcaaa gacaaaaatc
     6541 cgcacaataa taaaaaaccc tttcccattt gtattgaatt aatcggtcgc accatagaaa
     6601 tccttttcaa ggcagtatgt ttttttaata tcccggattc tgcgttatat ttttatttat
     6661 atcaatttcc ggctgaggca cgggccataa ataatttttt gacggatcaa atgttctctg
     6721 atccgagagg atacggtcag tggacagggt caaatggccg ttgttgggat cgacagtgcc
     6781 gagacgggaa ccatacaatg gcccggacat cacctgctcg ccaatcttcc aacgctgtat
     6841 atcaaaccac cggagacctt ccatggccag ttctaccctt ctttcccggc gtatcagttc
     6901 ccgcattttc gtctgggaat tatacagggt ctggtcaaca tccggcattc ctgcccggtt
     6961 acgcacctga ttgatggccg catacacggt accatcgatc tggttcagtt caattttcgc
     7021 ctcggcataa gttaacaaaa cttcggcata acgaatcagc ggaatgttca ggccgacatt
     7081 ccacatgtcg tcgaaatcag ataaatgaga aacgtacttt cttgatgcgt acccggtaaa
     7141 attatgccgg agccagtaat ccagggtccc cggctggagc ggattaaaat aaacgccatc
     7201 caccagcgaa cccggcacca ctacggtggc cgctaaccgc ggatcccggt tttgatatgg
     7261 ctggtccgga tcatagccgg aggacggatc gtcgatggtt ttcccattgc tcatttcata
     7321 agcatccacc agggattggg aaggatcaat ggaataccat ccgccgccgc cgcctccctg
     7381 gaaatgcaat gcatttcttc ccagcgaggt cagcggcgca tcgttttcaa tatactctat
     7441 atccagtatg atctcctggt tatgttcgtt ctggatacga aaaagatcgg tataattggg
     7501 gaacagcgaa tactttccca ttatattatt gcaggagctg atacagtctg cgtatttctg
     7561 gttatacagt tccgtccttg ctttcagggc ccaggccgcc ccgctggtga tccgtcccac
     7621 atccgctccg gtataactat cgggcagatc ggccgcaatg gatcccagtt catccaaaat
     7681 aaattgcacc acctggtcct tgggcgttct ggatacggta tctgcttcct gcgttgtaag
     7741 attggcggtg atcaaaggta catcaccata caactgggac ataataaaat acttgtatgc
     7801 cctcagaaat ctggcttccc ctttcatcct tttcttaagc ccgtcatcca tcggcgtttt
     7861 gtcgatattg gccagaaacc agttgcattt ctggatgctg ttgaaattcc atttattgtt
     7921 gggggtatgg ttgggagtaa gcaggaccat attcccatac tcttcatacc cttcccccgg
     7981 aaatgcgctg aacgcgttgt cggaagcgca gggcatataa aagatccagg tgccgtcttc
     8041 aaacccggaa taacatccgg tcaatgcggc actggcgtca ttggcggaag tccataagga
     8101 ggtttcgctg tactgatcga gtggctggcg gtctaaaaag ccttttttgc aggagctggc
     8161 gaatagggcc agaagactat atattaagat tgatttattt ttcatgacct gtttattttg
     8221 cgcatgtatt aaaaagtaat attgagacca accgtattga ttaatacctg gggataggtg
     8281 ttcccggatt caccggccgg agcttccggg tctatccagt tataaaaccg ggtggaagta
     8341 aaaatattct gaccgctgta atatatccgt gctttttgaa ttcccaccct ggccagccat
     8401 ccggaagaaa gggtataacc gacctgcagg tttttaagcc gcatataacc tgcattgcgt
     8461 acccaaaaag aagaattatt gtaatccgga tcattttgcg tgtaggaatt ccataagcgg
     8521 gggaattttg cattcggatt atcaggcgtc cagtgatcgc tgaagatgct cgtcggcttg
     8581 cccgtct
//
LOCUS       DASYCU010000317         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3258512, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000317 DASYCU010000000
VERSION     DASYCU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3258512"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VGM24_06425"
     CDS             <1..414
                     /locus_tag="VGM24_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155040.1"
                     /translation="LFFGFHTQKQGSVEPAWAQFRFVIFFFVEILSYSMIAVIFGLMI
                     RKAGLAMGIFFLYMIMEQFVVGLGRNKYKVGWVDYLPEEVTDRLIPQPFAPRMMSPAN
                     TTAWEHHIPLYLSLAVIYLLVYIVFVSWRFRKNDL"
     gene            468..1835
                     /locus_tag="VGM24_06430"
     CDS             468..1835
                     /locus_tag="VGM24_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4155041.1"
                     /translation="MKKIIFAGALVLISAAGFAQPRLPEHYYWEKMPNGLEVVVIEND
                     KVPLATIEIAVKNGAYTEGPEYSGLSHLFEHMFFKANKDYPDQEKLLARTQELGAIFN
                     ATTGAERVNYFFTFDKDSLSAGLRFMNAAIRFPIYRPEDMKKERPVVDGEFQRAESDP
                     GFQLWFECQKKLWGDLITRKNPIGIHEVINTATPEKMMIIKNKYYFPNNSILVICGDV
                     QHEDAFAKAQDIFGSWESSGFDPQIKFPIPEFTPLSSTEYFVKESSIAQTPLEMYFWQ
                     GPDTRHDSASTVAADVFFTILGMNSSKWQQALVDKGLTSFASAQYQTSRYVGPVMLYL
                     MPNPNKLKTCNDAILNQVKQWGNDDYFTDAQLEDAKQILRRNAIREREKPSAQASQVT
                     YSWCSTSLDYYTDYESNCQKVTRDDIKRFVNRYITGKPYVAGMIINPAMNKKFNASAE
                     FKPGL"
BASE COUNT          538 a          474 c          475 g          513 t
ORIGIN      
        1 ctgttttttg gtttccacac ccaaaaacag ggaagtgtgg aacctgcctg ggcccagttc
       61 cggtttgtga ttttcttttt tgttgagatc ctttcctatt ccatgatcgc tgtcattttt
      121 ggcctgatga tccggaaggc cggattggct atgggtattt tttttctgta tatgatcatg
      181 gaacagtttg tggttggcct gggaagaaat aagtacaaag tgggctgggt ggattatctg
      241 ccggaagaag tgaccgaccg gctgataccg cagccctttg cgccccggat gatgtctccc
      301 gccaatacca cggcatggga acatcacatc cctttgtatc tttcactcgc cgtcatttat
      361 ttgctcgttt atatagtttt tgtaagctgg agattccgta aaaatgatct ctagcgcaga
      421 tccgctcaat gatcagatat gaattcggta aacttaaaat tcctttaatg aaaaaaatca
      481 tcttcgcagg ggccctggtg ctaatatctg ctgccggttt tgcccaaccc cgcctccccg
      541 agcactatta ctgggaaaaa atgcccaacg gactggaagt cgtcgtcatc gaaaacgata
      601 aggttccgct ggccaccatt gagatcgccg taaaaaacgg agcgtacacg gaaggtcctg
      661 aatacagcgg gctttcccat ctctttgaac atatgttttt caaggcaaac aaggattatc
      721 cggaccagga aaaactgctc gccaggaccc aggagctcgg tgctattttt aatgccacca
      781 ccggcgcaga acgggtgaac tactttttca cctttgacaa agacagcctg tctgcgggac
      841 ttcgttttat gaacgccgcg atccgtttcc cgatttaccg gccggaagat atgaaaaaag
      901 aaagaccggt ggtggatggt gaattccagc gggctgaaag tgatcccggg tttcagttat
      961 ggtttgaatg ccagaaaaaa ctctggggcg atctgattac ccgcaagaat cccatcggga
     1021 tccacgaagt gatcaacacg gctacgccgg aaaagatgat gatcatcaag aataaatatt
     1081 atttcccgaa caactcgatc ctggtcatct gcggagatgt acagcatgaa gatgcctttg
     1141 caaaagcgca ggatattttc gggagctggg aatccagcgg atttgatccg cagatcaagt
     1201 tcccgatccc ggaattcact cccttgtctt caacagaata ttttgtaaag gaatcttcca
     1261 ttgcccagac tccattggaa atgtactttt ggcagggccc ggatacaagg catgattctg
     1321 cttccacggt ggcggcggat gttttcttta ctatcctcgg catgaactcc tccaaatggc
     1381 agcaggccct cgtggataag gggctgacca gctttgccag cgcacaatat cagaccagcc
     1441 gctatgtcgg ccccgttatg ctgtacctga tgcccaatcc gaataagttg aaaacctgta
     1501 atgatgccat actgaaccag gtaaagcaat ggggaaatga tgattatttc accgatgcgc
     1561 aactggagga tgccaagcag atcctgcgcc gaaatgccat ccgggaaaga gaaaaaccgt
     1621 ccgcgcaggc aagccaggtc acttatagct ggtgcagtac ctcgctggat tactatacgg
     1681 attatgaaag caattgtcag aaagttaccc gggatgatat caagcgcttc gtaaaccgct
     1741 acatcaccgg taaaccgtat gtggccggaa tgatcatcaa tcccgccatg aacaaaaaat
     1801 tcaatgcatc agcggaattc aaacctggtt tataattcat aaaattttca attatgtgca
     1861 ttcaacccgt tataaaaata tttttaagca gtatgctggg ggctgcttcc tttcttcccg
     1921 cggccgcaca gaataaagag ccctatgaaa tgacggtgaa cggtgtaaaa gtgatcgttc
     1981 aacccagtgg aaatgaaatt
//
LOCUS       DASYCU010000318         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1376803, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000318 DASYCU010000000
VERSION     DASYCU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376803"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VGM24_06435"
     CDS             <1..582
                     /locus_tag="VGM24_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY4155042.1"
                     /translation="QSRATFANYLRTLPYLDSSWKARPLALKTDSFLSAIPDSASRPV
                     LERTFTSANNLKSNFDLSALTYETERKNLRMHLMAWHEKITMSIACLVLFLIGAPLGS
                     IIRKGGIGMPLIFAVIFFVVFFLLNNFGRKFVKQDVLQPIAGMWMATYVLLPLGVFLT
                     YKAMNDSQLFNKEFYYRFFRNFRIKKKPEEKQP"
     gene            640..1653
                     /locus_tag="VGM24_06440"
     CDS             640..1653
                     /locus_tag="VGM24_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY4155043.1"
                     /translation="MLTIFSVDKGKQNLRVQLWIVIVALVLFSIKIVAWYLTRSVAIL
                     TDALESIVNVIAGFVGLYSLSIAYKPRDFNHPYGHGKAEFLSAAIEGTLIMLAGIFII
                     YEGVYHLFYPMKLQQLTSGIYLVAISAVINFIVGFIAVSKGKANRSDALEASGRHLQS
                     DTYSTLAVIVGLLLIYFTGYQQMDSIVAIIMSLVIMFIGYRIARKSVAGIMDEADDKI
                     LVKLIALLNRDRKPNWIDMHNLRVIKFGNILHIDCHMTVPWFLNVREAHAEVDTLRSL
                     IEKEFGPALEFFVHADYCIPDCCPICIKTDCSVRQHPFVKRIAWNMENIIPNKKHNSS
                     TAG"
     gene            1718..3085
                     /locus_tag="VGM24_06445"
     CDS             1718..3085
                     /locus_tag="VGM24_06445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEY4155044.1"
                     /translation="MQAWKQYQEENKDRFLEELFALLRIPSVSADSTRKPDMLACAEE
                     VKKSLLKSGAGKAEIFETAGHPVVYGEKIGDPSGPTVLVYGHYDVQPPDPLELWDSPP
                     FEPVIRDGKIFARGSADDKGQFFMHVKAMEIMQHSGKLPCNIKFIIEGEEEIGSPSLG
                     AFIHTHRDLLKAEVILISDTSMISLDTPSIDTGVRGLAYIQVELTGPNRDLHSGVYGG
                     AVANPITLLARMIASCHDENNHINIPGFYDDVLESSVEDRKKMAQAPFHEKEYMADLG
                     VKMLWGEKGYTTNERTGIRPTLEVNGIWGGYQGEGSKTVLPSKAFAKISARLIPNQSA
                     QKIAKLLMDYFKKIAPPSVTVNAFELHGGEPYLTPLDSKAYRAAEKAIEASFGKKPVP
                     VRGGGSIPICSLLEKELGIKIVFMGFGLDSDNLHSPNEKFDLANFYKGIETIPYFHQY
                     FAGMK"
     gene            3108..3509
                     /locus_tag="VGM24_06450"
     CDS             3108..3509
                     /locus_tag="VGM24_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY4155045.1"
                     /translation="MPEKEKLRIDKYLWAIRLFKTRALASAACEKGKIKFNSAQVKPS
                     RSVSLGDEYEVKTEARRWRIKVSGLLDHRVGHAEAIQNYLDITPAEEVERLQFQAASF
                     HTGKRLSKVGRPTKKLRRDWEDFREEDRDAP"
     gene            3518..4216
                     /gene="trmD"
                     /locus_tag="VGM24_06455"
     CDS             3518..4216
                     /gene="trmD"
                     /locus_tag="VGM24_06455"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY4155046.1"
                     /translation="MPLISFLMRIDIISVLPELLESPFAHSILKRAKEKQLLEIFVHP
                     LRSWAINEYGQVDDYQYGGGAGMVMMCEPLANAIDSLSGERKYDEIIYLTPDGELLNQ
                     PIVNALSLKNNLMIISGHYKGIDERIRKHFITKEISIGDYVLSGGELPAAVLADAIGR
                     LIPGVLNDETSALFDSFQDNLLAPPVYTRPAEFRGWKVPDILLSGDPAKIADWRQEES
                     LKKTEQRRPGLLND"
     gene            4227..>4841
                     /locus_tag="VGM24_06460"
     CDS             4227..>4841
                     /locus_tag="VGM24_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4155047.1"
                     /translation="MMISGQLVDIPGKRIFPAELQVQNKKITAIQELPSDENVPKQYV
                     CPGFIDAHVHIESSMLVPAEFARLAVVHGTVATVSDPHEIANVCGMEGVDFMIQNGRD
                     SPFHFYFGAPSCVPATVFETAGATLNAKQVEALLRRPEIRYLSEMMNFPGVLQGDPEV
                     MDKIASAKKYDKPVDGHAPGLRGQDAKKYIAAGISTDHECFTADE"
BASE COUNT         1268 a         1205 c         1208 g         1160 t
ORIGIN      
        1 cagtccaggg ccacttttgc aaactatctc cgtaccctgc cctatctgga cagttcatgg
       61 aaagcccgcc ccctggcatt aaaaacggat tcctttctgt ccgccattcc cgattccgcg
      121 tccagaccgg tactggaaag aacgttcacc agcgccaaca acctgaagtc gaactttgat
      181 ctgtccgccc tcacttatga gaccgaacgc aaaaacctgc gaatgcacct gatggcctgg
      241 catgaaaaga tcaccatgtc cattgcctgt ctcgtccttt ttctgattgg cgcaccgctg
      301 gggtccatca tccgcaaagg agggataggt atgccgctga tatttgccgt gatctttttt
      361 gtggtctttt tcctgctcaa taatttcgga aggaaatttg tgaagcagga tgttctccaa
      421 cccatcgcgg ggatgtggat ggcaacctat gttttgctgc ccctgggcgt tttcctcacc
      481 tataaagcca tgaacgattc acaacttttc aataaggagt tttattaccg ttttttcagg
      541 aatttccgga ttaagaaaaa accggaagaa aaacagccct aggtctttgt gcagaactta
      601 ttttgacccg ggaaaccgtt ctacctgatt cctttacatt tgctgacaat ttttagtgtg
      661 gataaaggca aacaaaatct ccgggtacag ttatggatcg tgattgtagc cctggtcctg
      721 ttcagcatta aaatcgttgc ctggtatctc acgcgctcgg tggccattct gacggatgcg
      781 ctggagagta tcgtgaatgt catcgccgga ttcgtgggcc tgtacagctt atcgatcgct
      841 tacaaacccc gcgatttcaa ccacccctat ggtcatggaa aagcggaatt tctatctgca
      901 gccattgaag gcaccctgat catgctggcg ggaatcttca tcatctatga aggggtttat
      961 catctgtttt atcccatgaa attgcagcag ctgacctccg gtatttacct ggtggccatt
     1021 tctgccgtta taaactttat tgtcggattt attgcggtca gcaaagggaa ggcaaaccgg
     1081 tcggacgcgc tggaagccag cggccgtcat ctccagtcgg acacctattc cacgcttgcc
     1141 gtcatcgttg gtttactgct catttatttt acgggttacc agcagatgga cagcatcgtt
     1201 gccatcatca tgtcgctggt gatcatgttc attggttacc ggatagcgcg aaaatccgtc
     1261 gccggcatca tggatgaggc ggatgataaa atcctggtaa aactgattgc gctgctgaac
     1321 cgggaccgga aacccaactg gattgacatg cacaacctcc gggtgatcaa attcggcaat
     1381 atcctgcaca tcgactgcca tatgaccgtt ccctggttcc tgaacgttcg cgaggcgcat
     1441 gccgaagtgg acacgctccg gtcattaatt gaaaaagaat ttggtccggc actggaattt
     1501 tttgttcatg ccgactactg cattcccgat tgctgtccca tctgcatcaa gacggattgc
     1561 tccgtgcgcc agcatccgtt tgttaaacga atagcctgga atatggaaaa catcatcccc
     1621 aataaaaaac acaatagctc aacagccggc tgatctctta aaaggagcta atttcgcgct
     1681 gcccgcttac tctttgcggt ttattaaaat ttcacccatg caagcctgga aacaatatca
     1741 ggaggagaat aaagacagat ttctggaaga attattcgca ttgctccgga tcccttcggt
     1801 gagtgcagac agcacgcgga aacccgacat gctggcctgt gcggaagaag taaaaaaatc
     1861 cctgctgaag tccggggccg gaaaggctga aatattcgaa accgccgggc atcccgtggt
     1921 atacggcgaa aagatagggg acccttccgg accgacggta ttggtttacg ggcattacga
     1981 tgttcagccg cccgatccgc tggaactttg ggacagtccg ccctttgaac ccgttatccg
     2041 ggacggaaag atctttgcgc gggggtccgc ggacgataag ggacagttct tcatgcatgt
     2101 gaaggcaatg gagatcatgc agcattccgg caaactgccc tgtaatatca aattcataat
     2161 tgaaggagag gaagaaatcg gttcacccag cctgggcgca ttcatacata cgcaccggga
     2221 tttattaaag gccgaggtca tcctgatcag cgatacttcc atgatcagtc tggatacccc
     2281 ttcgatagat accggcgttc gcggtttagc ctatatccag gtggaattga ccggccccaa
     2341 ccgggacctg cacagcgggg tttacggggg tgccgttgcc aatcccataa ccctgctcgc
     2401 ccggatgatc gcaagttgtc atgatgaaaa taaccatatt aacatccccg gattttatga
     2461 tgatgtgctg gaatcctccg tagaagacag aaaaaaaatg gctcaggctc ctttccatga
     2521 aaaggagtat atggccgatc tgggcgtaaa aatgctttgg ggagaaaaag gatatacgac
     2581 caatgaacga accgggatcc ggcccacgct tgaagtgaac gggatctggg gaggttacca
     2641 gggcgaggga tccaaaaccg ttttaccatc caaagccttt gctaaaattt cagccaggct
     2701 tatcccgaac caatccgctc agaaaatagc caaactgctg atggattatt ttaaaaagat
     2761 cgctccgcca agcgtgaccg taaatgcctt cgaattacac ggcggcgaac cctatctcac
     2821 gccattggat tccaaagcct acagagccgc agaaaaagcg atcgaagcca gctttggtaa
     2881 aaaaccggtg cccgttcgcg gcggcggcag tatcccgatc tgttcccttc ttgaaaaaga
     2941 actcggcatc aagattgtat ttatgggatt cgggctggac agcgacaacc tccattctcc
     3001 caatgaaaaa tttgatctcg ccaattttta taagggcatc gaaaccattc cttactttca
     3061 tcagtacttt gccgggatga agtaaactac gggtatccga attcattatg ccagaaaaag
     3121 aaaaactcag gatcgataaa tatctctggg ccatccggct ttttaaaacc agggccctgg
     3181 cttcggctgc ctgtgaaaaa ggaaaaataa aattcaattc ggcccaggta aaaccctcca
     3241 ggtccgtcag cctcggggac gaatacgaag taaaaacaga agccagaaga tggcggatca
     3301 aggtaagcgg gctgctggac caccgggtgg gccacgcaga agccatccaa aattacctcg
     3361 atatcacgcc ggcggaagaa gtggagaggc ttcagttcca ggcagcgagc tttcataccg
     3421 ggaaacggct gagtaaagtg ggaaggccca ccaaaaaatt acgcagggac tgggaggatt
     3481 tccgggagga ggatcgggac gctccttaaa tctatctttg ccactcatct cttttctcat
     3541 gcgaatagat attatcagcg tgttgcccga attactcgaa agtccttttg cgcattccat
     3601 attaaaacgc gcaaaggaaa aacaactgct ggaaatattt gtgcacccgc tgcggtcctg
     3661 ggccattaat gaatatggcc aggtggatga ttaccaatat gggggcggcg ccggtatggt
     3721 gatgatgtgc gagcccctgg ccaatgccat cgactcgctt tcgggagaaa gaaaatatga
     3781 tgagatcatt tacctgacgc ccgatgggga actgctgaat cagccgatag tgaatgctct
     3841 ctcgctgaaa aataatctga tgatcatttc cggccattat aaagggattg acgaacgcat
     3901 ccgaaagcat ttcataacaa aagaaatctc gatcggcgat tatgtgctga gcggcggcga
     3961 acttcccgca gcggttctcg ccgatgccat cggcagattg attcccggcg tgctcaatga
     4021 tgaaacctct gccctatttg attcttttca ggataacctg cttgctccac cggtctatac
     4081 ccggccggca gaattccggg gctggaaagt gcccgatatt ctgctcagcg gggatccggc
     4141 aaaaattgct gactggcggc aggaggaatc cttaaaaaaa acggaacaaa gaagacccgg
     4201 attactgaac gactgaatac cacattatga tgatcagcgg gcaattggtt gatatccccg
     4261 gtaaaaggat ttttcctgcc gaactgcagg tgcagaataa aaaaattacg gctatacagg
     4321 aactgccttc ggatgaaaac gttccaaaac aatatgtctg tcccggtttt attgatgccc
     4381 acgttcatat tgagagttcc atgctggttc ctgccgagtt tgcccggctc gccgtggttc
     4441 atggtacggt ggctaccgtg agcgatcccc acgaaatcgc gaatgtttgc ggaatggaag
     4501 gagtggattt tatgatccaa aacgggcggg attccccctt tcatttttac ttcggcgcac
     4561 ccagttgcgt cccggctacc gttttcgaaa cggccggggc cacgctgaat gcgaaacagg
     4621 tggaagccct gctgcgccgc ccggagatcc ggtacctgag cgaaatgatg aattttcccg
     4681 gggttttaca gggagatccc gaagtgatgg ataaaatagc ttctgctaaa aaatacgata
     4741 agccggtaga cgggcatgct cccggcctgc gcgggcagga tgcaaaaaaa tatattgcgg
     4801 ccggtatcag caccgatcat gaatgtttca cggccgacga g
//
LOCUS       DASYCU010000319         6709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3114936, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000319 DASYCU010000000
VERSION     DASYCU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3114936"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1334
                     /locus_tag="VGM24_06465"
     CDS             21..1334
                     /locus_tag="VGM24_06465"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HEY4155048.1"
                     /translation="MVDSRIKWDVNLASAGIFVDNSFFYIPGRQLPPFGFKKIADEIN
                     NRRVRSHFNPSRPNQLYHFVSSAETIGPSFFMPLNYNSQIGFTIAARGYANLMQVPGH
                     LAQNTYTNLKDPSLWNTEWQDTHSKVNSMAWLEYGLHYGRVLYRRNNKKLLMGVSLKY
                     LQGIVAAYAKNTNLNYTIIDSNEAMLSHSSVDYGGTREFSGKKYSDLIHGSGFAGGLG
                     LTFVSGGPSACNANPDAITENESGNRYVYKIGISLIDLGNIRFNRYSAAYYLRADSAV
                     YTGNGQSNSGSSGPIAQFVYENDSAKHLTANHFNMVLPSAISVQADWNVFNDYYINAT
                     VIKGFGHGNHTGVVRPDLYSVTPRYETSWFEVSAPFSLLYYQRWQPRIGIAFRIGYFF
                     FGGDAPASLAGIGNFERTDIYAGIHFFPLEWKHRPSGLLCPDVKN"
     gene            1456..2493
                     /locus_tag="VGM24_06470"
     CDS             1456..2493
                     /locus_tag="VGM24_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY4155049.1"
                     /translation="MENDDFELNPFFELTPDLVCVAGKDGYFRKINHAVIHKLEYSRE
                     ELFSRPISSFIHPEDRKITEQERKELLGGKPLINFENRYISKSGKIIWLHWTSVYIPD
                     REVVFAISKDVTERKTIDREIEEKYKKFKSLTTYYKSSIEKDRKYLAIELHEELAQLA
                     SVVKMDIDWIENNSPDLSALTKNRIGHALSVSDLLISAIRRISFSISPRMLDDLGLNE
                     TLIWLCSEFAILNGIPCEFESDYNEADLTHEIKLDFFRICQESLTSVMYHVLASSVKI
                     SIVASEDKICLSIFDNGEGFNIEQQNQTSVWTGISERVASINGHLTIKNEFGKGTTIQ
                     VSINKPPLSTQ"
     gene            2515..3306
                     /locus_tag="VGM24_06475"
     CDS             2515..3306
                     /locus_tag="VGM24_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY4155050.1"
                     /translation="MGKNESSRRKFVKLSSIGLGGMAFSPFLKISKKDSGFHPSGGTG
                     KKMNILCVGAHPGDPEFGCGGTLARYSDAGHQVSILYLTRGEAGDPSKTFEQSAALRT
                     KEAETACKILKARAIFAGQIDANTELNKSSIEKITKLVLSEKPDVLFTHWPVDAHPDH
                     QVSGLLAFNAWIKSGRQFELYYYEVNTGSETLEFKPGEYVDITEVRDRKKQAMFAHKT
                     QNPEQVYADFFKTMEEFRGLEAGVKAAEGFIRAKLKEERANISGL"
     gene            3424..3852
                     /locus_tag="VGM24_06480"
     CDS             3424..3852
                     /locus_tag="VGM24_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155051.1"
                     /translation="MKKLILIAGFMPFFGKAQFKIVNTATINLLELRSDTWPINLQRV
                     TKQSDTCYVLNFRTQEFSNARIMKSLTFRNLEQLRYFEKALSALKTGATGDVAEFKDY
                     TIKRVDVKQDGIWYTLNYDNGLTNFKQSEADKMIAAISKL"
     gene            complement(3983..4132)
                     /locus_tag="VGM24_06485"
     CDS             complement(3983..4132)
                     /locus_tag="VGM24_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155052.1"
                     /translation="MIRKLKSGEYRLYSRKANPKTKKRRNLGTFSSLEAAKKHEKEIQ
                     YFKRH"
     gene            4216..5334
                     /locus_tag="VGM24_06490"
     CDS             4216..5334
                     /locus_tag="VGM24_06490"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEY4155053.1"
                     /translation="MKYVLYFLIPFSVLSHAGTAQQLKPGFDRDEYIALMKLSAQFGD
                     SSYAASMSAPKEYRLLYRSPVMGLLNRWDLWMRDDGIAVISIRGTIPDKISWLANFYA
                     AMVPAKGMLQLSKTDSFSYELADNPRAAVHVGWLVSMAFLSETILPKIDSLYKTGTRE
                     YLLIGHSQGGGIAYLLNAYLFHLQKEKRLPADIRFKTYCSAAPKPGNLYFAYDYETAT
                     QGGWAYNVVNAADWVPEAPVSIQTLDDFNAVNPFKHIIPFIKKQKWPGRWALRYAYGR
                     MNKPNRKAARNYRKYLGTFVSKSIKKQLAGFNPPKYDNSMNYARAGSYIVLEPDEDYY
                     KRFPQDSGNIFINHMHPPYLFLIGKFSMPSGTNRTSTR"
     gene            complement(5363..5581)
                     /locus_tag="VGM24_06495"
     CDS             complement(5363..5581)
                     /locus_tag="VGM24_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155054.1"
                     /translation="MKKFLLIGCIVIAGQACSTTQPMSVTANPDPTTSTSGTASSAKF
                     TDRMPRKSTDTATLPTDTARIKREAVPR"
     gene            5744..6460
                     /locus_tag="VGM24_06500"
     CDS             5744..6460
                     /locus_tag="VGM24_06500"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEY4155055.1"
                     /translation="MKNKTPVVLSWSGGKDSSYALHQLMNDERYEICYLLSTINGMNK
                     RLSMHGVREELMDIQAAAIGIPLIKTFVCKGTNEEYEQQMGDSLRKLKAEGIHAIAFG
                     DIFLEDLRSYRENQMRQVGMECLFPIWQKDTLGIVNDFIAKRFKAYTCCVNSSRLDES
                     WCGRLIDEGFVKDLPPSADPCGENGEFHSFCFQGPVFKRPLSIITGEKVYKPMDRPAG
                     DPDTHFTGTIGFWYCDLLPS"
     gene            complement(6492..6689)
                     /locus_tag="VGM24_06505"
     CDS             complement(6492..6689)
                     /locus_tag="VGM24_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155056.1"
                     /translation="MRNRDSGILYLILGIYSILVSWYYNHSIILLLVHWIFWPVYLVY
                     ELLIGHLSHGMWKTIPLSYFN"
BASE COUNT         1881 a         1521 c         1572 g         1735 t
ORIGIN      
        1 tatttctaaa tcccgcttta atggttgatt ccaggataaa gtgggatgtc aatttggctt
       61 ctgcgggtat atttgtggac aattcctttt tttatatacc ggggcggcaa ctgcctcctt
      121 tcggattcaa aaaaatcgcc gatgagataa ataacaggcg ggttcggagc cattttaatc
      181 cctcgcggcc caatcagctg taccatttcg tatcatcggc cgaaaccatt gggccatctt
      241 tttttatgcc cctgaactat aattcacaaa ttggattcac gatagccgcc aggggttatg
      301 cgaatcttat gcaggtgccc ggccacctgg cccaaaatac ctatacgaac ttgaaagacc
      361 cgtccttatg gaatacggaa tggcaggata cgcattcgaa agttaattcg atggcctggc
      421 tggaatacgg gctgcactat gggcgggtgc tctatcgccg caataataaa aagttgctca
      481 tgggcgtcag tctgaaatat ttgcagggaa tagtcgccgc gtatgcgaaa aatacgaacc
      541 tgaactatac cataatcgat tcgaacgagg ccatgctttc ccattcctct gtggattatg
      601 gcggaacccg ggaattctcc ggtaaaaaat acagcgattt aattcacgga tctggatttg
      661 ccggaggcct tggtctgacc tttgtgtcgg gcggtccctc cgcctgcaat gcgaacccgg
      721 acgccatcac ggaaaacgaa tccgggaacc gttatgtata taaaataggc atttccctaa
      781 ttgacctggg taatatccgt ttcaacagat attcagccgc ctattatctc cgggcagaca
      841 gcgctgtata tacgggaaac ggacagtcta attccggttc ttccgggccc atagcccaat
      901 ttgtttatga aaatgattcc gccaaacatt tgactgcaaa tcattttaat atggtcctgc
      961 cgtctgcgat aagtgttcag gcagactgga atgtttttaa tgattattat attaatgcaa
     1021 cggtcataaa gggctttggg catggcaatc acaccggcgt ggtaagaccg gatctctact
     1081 ccgtaactcc tcggtatgaa acgagctggt tcgaggtgag cgctccgttc tctcttttgt
     1141 actaccagcg ctggcaaccg aggatcggca tcgctttcag aattggatat ttcttttttg
     1201 gcggagacgc tcccgccagc ctggccggga ttggcaattt tgaacgaacg gatatttatg
     1261 ccggcattca ttttttcccg ctggaatgga agcatagacc ttccggcctg ttatgccccg
     1321 atgttaaaaa ttaggtggcc gtttgcgaaa aaagccaatg aagatggctg cttgctaatc
     1381 agcccgcata tgttaagttc gcgcaaatat tgagagtttt atatagattt atatcattta
     1441 aaaaatatac ccgatttgga aaacgatgat tttgaactga atccattttt tgaactcact
     1501 ccggacctcg tatgcgtagc aggcaaagat ggctatttca ggaagataaa tcatgcggtc
     1561 attcataaat tggaatattc ccgggaggag ctgttttcca ggcccatctc ttcctttatt
     1621 catccggaag accggaaaat caccgagcag gaaaggaagg aattactcgg tggcaaaccg
     1681 ctgataaatt ttgaaaaccg gtatatttct aaatccggaa aaatcatctg gcttcactgg
     1741 acctctgttt atatcccgga cagggaagtc gtcttcgcca tctccaagga tgttacggaa
     1801 aggaaaacga tagaccggga aattgaggaa aagtataaaa aatttaaaag cttaactacc
     1861 tattataaat ccagtattga aaaagacaga aaatatttag ccatcgagtt gcatgaggag
     1921 ctcgcgcaat tggcttctgt tgtcaaaatg gatatagact ggatagaaaa caacagccct
     1981 gatttatcgg ccctgaccaa aaacaggatt gggcatgcat tgtcggtgtc tgacctgctc
     2041 attagcgcaa tccggagaat ttcgttttcg atcagcccca ggatgctgga cgacctggga
     2101 ctgaacgaaa cgctgatctg gctttgcagt gagtttgcca ttttaaacgg aattccctgc
     2161 gagtttgaaa gtgattataa tgaagcggac ctgacccatg aaatcaagct ggactttttc
     2221 cggatctgcc aggaatccct taccagcgtg atgtatcacg tactggccag ttcggtaaaa
     2281 atcagcatcg tggcttcgga ggataaaata tgcctctcca tttttgataa tggggaagga
     2341 ttcaacatcg aacagcagaa ccaaacatcc gtatggaccg gtatcagcga acgggtcgcc
     2401 tcgatcaacg ggcatttgac gattaaaaat gaattcggaa agggaaccac aatacaggtt
     2461 tccataaata aacctccgct gtcaacgcaa tgaacccgaa aacaatttaa cctcatgggc
     2521 aaaaatgaat ctagccgcag gaagttcgta aaactttctt cgatcggact tggaggaatg
     2581 gctttttctc cgttcttaaa gatctcaaaa aaggattccg gattccatcc ttccggaggt
     2641 accggaaaaa aaatgaatat cctctgtgtg ggagctcacc ccggcgaccc ggaattcggt
     2701 tgcggaggaa ccctggcgag atatagcgat gccgggcacc aggtaagcat cctttatctt
     2761 acccgcggtg aagcgggaga cccgtccaaa accttcgaac aatcggcggc attgagaacg
     2821 aaggaagccg aaacggcctg taagatatta aaggcaagag ccatttttgc cggccagatc
     2881 gatgccaata cggaattaaa caaatcgagc attgaaaaaa taacaaagtt ggtgctatcg
     2941 gagaaaccgg atgttctatt tacccattgg ccggtggatg cgcatcccga ccaccaggtt
     3001 tccggtttac tggcattcaa tgcctggata aaatccggcc ggcaattcga actgtattat
     3061 tatgaagtga atacaggaag cgaaacgctc gaatttaaac cgggtgaata cgtggacatc
     3121 acggaagtga gggataggaa aaagcaggcc atgtttgcgc acaaaactca aaatccggag
     3181 caggtatatg cagatttttt caaaaccatg gaggaattcc ggggtttgga agcgggagtg
     3241 aaagcggccg aaggttttat tcgtgccaaa ttaaaggaag aaagagcaaa tatcagcggg
     3301 ttgtaattat aatacatcgc ggttttaatc gttatatcgg ggcgagactt cccaaaaacc
     3361 ggatccctta aaatttaact atttattcta taatacggat ttcctttaaa aacaggttct
     3421 gatatgaaaa aacttattct gatcgccgga ttcatgccct tttttggaaa agcacagttt
     3481 aaaattgtca atactgccac catcaatctg ctggaacttc gttccgatac ctggccgatt
     3541 aatctgcaaa gagttacaaa acaatcagat acctgttatg tgctcaattt ccgcacccag
     3601 gaattttcaa atgcccggat catgaaaagc ctgacctttc ggaatctgga acaattgaga
     3661 tattttgaga aagctttatc tgctttgaag accggggcaa ccggcgatgt ggcggaattc
     3721 aaggattata ctatcaaaag agttgatgta aaacaggacg gcatctggta tacgctgaac
     3781 tatgataatg ggctgaccaa ttttaagcaa tccgaggccg ataaaatgat cgctgcgatc
     3841 agtaaactat aatccctccc ggattaattc aatccaatca caggagacag ggacagcgct
     3901 gaaccataaa caaattcttt ccttatccat atcaaaatgg ataaggaaaa cctgtattta
     3961 tagctgccgg aattcccggt gctcaatgcc gtttgaaata ctggatctct ttctcgtgct
     4021 tcttggcggc ttccaatgaa ctgaacgttc ccaggttgcg gcgtttcttt gtcttcggat
     4081 tggctttgcg ggaatagaga cggtattccc cggattttaa ttttcggatc ataatgcttt
     4141 tttggatgaa attgcaaaga tgatgcccgc gaaaagccgt ccatccggta cattcgcaaa
     4201 taaaaaattt cacacatgaa gtacgtgctg tattttttaa tccccttttc cgttctctcg
     4261 catgccggca cggcccaaca actcaaaccc ggatttgacc gggatgaata tatcgcctta
     4321 atgaaactct ccgcgcaatt cggcgattcc agctatgcag ccagcatgtc cgcgccaaag
     4381 gaataccgcc tgctttaccg ttccccggta atgggcctgc tgaaccgctg ggatttgtgg
     4441 atgcgggatg acggaatagc cgttatcagt atccgcggca ccatccccga caaaataagc
     4501 tggctggcaa atttttatgc ggcgatggtt ccggccaaag gcatgctgca actttccaag
     4561 acggacagtt tttcctatga gctggcggat aatccacgcg ccgctgtgca tgtcggctgg
     4621 ctggtcagca tggccttcct ttctgaaacc atcctgccaa aaattgattc cctgtataaa
     4681 acgggtacaa gagaatatct tctgattgga catagccagg gaggcgggat tgcttacctg
     4741 ctgaatgcct atttattcca tcttcaaaag gaaaaacggc tccccgccga tatccggttc
     4801 aaaacgtatt gcagtgcggc gcccaaaccg ggaaatctct attttgccta cgattatgaa
     4861 acggccacgc agggcggctg ggcttacaat gtggtgaatg cggccgactg ggtgccggaa
     4921 gcaccggtct ccattcaaac gctggatgat tttaatgccg tgaatccgtt caaacatatt
     4981 atccccttca tcaaaaaaca aaaatggccg ggtcgctggg ctttgcgtta tgcttatggc
     5041 cggatgaata aacccaaccg gaaagccgca cggaattatc ggaaatacct gggaacattt
     5101 gtttcaaaat caattaaaaa gcagcttgcc ggatttaatc cacccaaata tgacaacagt
     5161 atgaactatg cccgcgcagg atcttatatt gtattggagc ccgatgaaga ttattacaaa
     5221 cgatttccgc aggacagtgg caatattttt ataaatcaca tgcacccgcc atatctattt
     5281 ctgatcggga agttttctat gccctccgga acaaaccgga cgtccacccg ttaagatcag
     5341 ggattcagcc agggtcggca ccttaacgcg gaaccgcctc cctttttatc ctggccgtat
     5401 cggtaggcag tgtggccgta tcggtggatt tacgcggcat ccggtcggta aatttagccg
     5461 aactggctgt tccggaggtc gatgtggtcg gatccggatt ggccgtaacg gacatgggct
     5521 gggttgtgct gcaggcctgt ccggcaatta caatgcagcc tatcagaagg aattttttca
     5581 tgatcattgg ttattcctta tcctttcaat tttcatacct ctgttagtga acggggccgg
     5641 cgtccctggc acgattttct gcttattatt cccggcaaat aaatccaccg ggcaccaggc
     5701 cgggttttaa aacaattaca tttgcatttt ttcattttga agcatgaaaa acaaaacacc
     5761 ggtcgtatta tcctggagtg gcggaaagga cagttcctat gccctgcatc agttgatgaa
     5821 tgatgaacgc tatgaaattt gctatttact gagcaccatt aacggaatga acaaaaggct
     5881 atccatgcat ggagtgcggg aagagctgat ggacatacag gcagcagcta ttggaattcc
     5941 tttgataaaa acatttgtct gcaaaggaac taacgaagaa tatgaacagc agatgggcga
     6001 ttccctgcgg aagctgaaag cggaaggtat ccatgccatt gccttcggcg atattttcct
     6061 ggaagacctg agatcatacc gggaaaatca aatgaggcag gtcgggatgg aatgcctttt
     6121 cccgatctgg caaaaagata cgctggggat cgtcaatgat tttattgcca aaagatttaa
     6181 ggcatatacc tgttgcgtca acagcagccg cctggatgaa agctggtgcg ggagactgat
     6241 cgatgaaggt tttgtaaaag accttcctcc ttccgccgat ccctgcgggg agaacggtga
     6301 gttccatagc ttctgctttc agggtccggt ttttaaaaga ccattatcca taattaccgg
     6361 agagaaagtt tacaaaccca tggatcggcc tgccggtgac ccggataccc atttcaccgg
     6421 caccatcggt ttctggtatt gcgatctgct tccttcctga ggaaatcaca atcccggcgt
     6481 ggtagccgct ctcaattaaa atacgacaaa gggatggtct tccacatacc atgcgaaaga
     6541 tggccgataa gtaattcata taccaggtat accggccaaa agatccagtg cacgagcagc
     6601 aaaatgatgg aatgattata ataccagctg accagtatgg aatagatacc taaaataaga
     6661 tataagatgc ccgaatccct gttcctcata aaaggagttt ctgtttact
//
LOCUS       DASYCU010000320         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1377530, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000320 DASYCU010000000
VERSION     DASYCU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377530"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VGM24_06510"
     CDS             <1..295
                     /locus_tag="VGM24_06510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4155057.1"
                     /translation="TIEDDVTITTMCVVIAHDLSRKYNEGKEIIGAVLIRKGAFIGMN
                     TVIMPGVTIGEGAVVAAGSVVTKDVEPFSVYGGVPAKKIRDYVLNEKKNSQTG"
     gene            complement(296..895)
                     /locus_tag="VGM24_06515"
     CDS             complement(296..895)
                     /locus_tag="VGM24_06515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4155058.1"
                     /translation="MKTTSLKGRGLFHVLDSFHLRLKLLFGKYYFFMSRCRGVKFLGS
                     CQFIGRPKFKLYPGTKLIIGNNCKFISGHTDNLIGINHPCIITVSNAGTELRIGENSG
                     FSGTAIGAFAGITIGSNVKCGANTLITDSDWHPEDPRSGRPSPIFIGDNVWLGVNCVV
                     LKGVRIGDNAVIGANSVVSRDIPPNSVAAGNPCKVIREL"
     gene            complement(892..2199)
                     /locus_tag="VGM24_06520"
     CDS             complement(892..2199)
                     /locus_tag="VGM24_06520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4155059.1"
                     /translation="MEKSEKRLRVLWFANTPGLSQPLLNDRTSGGGWISSLQSGVENS
                     LRVDLGFVFYSDLQLDPFHLNKTDYYPIRRTAFTKSKRLLRRISGRAEYEENLARFLR
                     IIKEFKPDIIHVHGTENSFGLIVKQVHDIPVIISIQGNLTVYARKYFSGISRPGLIRQ
                     WRSGYPFFGADFRIWGKRARIEREILKKARYVFGRTDWDRRICLLMAPGARYFHIEEV
                     MREKFYRIRWEPLPDSSPVFFTTCSASFYKGFETILDTARILSDHRLPFTWIVAGLKE
                     EDALVRLVKKIKKIDRLEDLNVRLKGPLSADELANGLLQANVYVQVSHIENSPNSLCE
                     AMLAGMPVIASFAGGTASLVQDNITGILVQGGDPYVLAGALLEMIKYPQNSIDMAREA
                     YRTAHQRHDPPAIVDRLLSAYSEIIEEHQAGGLPTIHKSRING"
     gene            complement(2227..>3337)
                     /locus_tag="VGM24_06525"
     CDS             complement(2227..>3337)
                     /locus_tag="VGM24_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY4155060.1"
                     /translation="TYYYKLVGVNDQGKETTASNIIAVLTPDPAADRFVYIGSEDKNM
                     YCFYAGTGAKVWAFPTNGDVESSATIKGGVLYFGSTDQRLYAIDALTGVRQWNFLTEG
                     AILTSPATGTDAIYIASYTGFVYGVNMNGSKKWSAQPSGPSLFFSSPVYSNGSIYIGS
                     PDHSLYAFDAETGTKLWSAATSDTINSSPAISNGIVYVGSSDKYLYAFDAVTGSFKWK
                     FATGDSVLSSPTISNGTGYVGSFDGKLYAFNAVSGAIKWSTATGGRIQSSPAVSNGVV
                     YAGSFDGKLYAFDAVTGAVKWSTATGDRIMSSPTVSNGVVYAGSYDNKLYAFSVSDGT
                     IKWTAATGGKIRMSSPTVLTFPGNIVLPGVSGDVQ"
BASE COUNT          827 a          854 c          812 g          844 t
ORIGIN      
        1 caccatagaa gacgatgtta cgattaccac gatgtgcgtg gtgattgccc atgatctgtc
       61 ccgcaagtac aatgaaggca aggaaattat tggagcggtg cttatcagga agggcgcatt
      121 catcggaatg aataccgtca ttatgcccgg agtaaccatc ggggaaggcg ctgttgttgc
      181 tgccggttcc gtggttacca aagacgtaga gccttttagc gtatatggag gagtgcctgc
      241 aaaaaagatc agggattatg tattgaatga aaagaaaaat tcgcagaccg ggtagtcata
      301 actcacggat cactttacag ggattgcctg ccgcaacgga attcggggga atatccctgg
      361 aaaccacgct atttgcaccg atcaccgcat tgtcgccgat ccgtacgcct tttaaaacga
      421 cgcaatttac tcccagccac acattgtctc cgataaatat gggtgatggc ctgccgctgc
      481 gcggatcttc gggatgccag tcggaatcgg ttatcagcgt attggctccg catttcacgt
      541 tgctgccgat cgtaattccg gcgaatgctc cgattgcggt tccgctaaac cctgaatttt
      601 caccgatccg caattctgtt ccggcattcg ataccgtaat gatacaggga tgattgatgc
      661 cgatcaggtt gtcggtatgt ccggaaatga atttgcagtt attgccaatg atcagcttcg
      721 ttcccggata aagcttaaac ttcggtctgc ctataaattg gcaggatccc aggaatttga
      781 cgccccggca cctgctcata aaaaaataat atttcccgaa caggagcttt agtctcaggt
      841 ggaatgaatc taaaacatgg aacaggcctc ttccttttaa tgaggtggtt ttcatccgtt
      901 gattcttgat ttgtgaatgg ttggaagacc acccgcctga tgttcttcaa ttatttctga
      961 ataagcggac aataaacggt caacgattgc cggcggatca tgtctttgat gcgcggtccg
     1021 gtatgcctcc cgggccatgt caatggaatt ctgcggatat tttatcatct ccagaagcgc
     1081 tcctgccagc acatacggat ccccgccctg taccaggatc ccggttatat tatcctgaac
     1141 cagggaagcg gtacctcctg caaaagaggc aatcaccggc atgccggcca gcatggcttc
     1201 acaaagactg ttcgggctgt tttcgatatg ggaaacctgc acgtacacat tcgcctgcaa
     1261 aagcccgttt gccagttcat ccgcggacaa gggtcctttc aaccggacat tcagatcctc
     1321 cagccggtct atcttcttta tttttttgac cagacgcacc aacgcatctt cttcctttaa
     1381 tcccgctact atccaggtaa atggtaaccg gtggtcagac agaatcctgg ccgtgtcaag
     1441 aattgtttca aaccccttgt agaaagaagc gctgcaggtg gtgaaaaaaa cgggagaact
     1501 atccggaagc ggttcccacc tgatccggta gaacttctcc cgcatcactt cttctatatg
     1561 gaaataccgg gctccgggag ccatcagcag gcaaatgcgc cggtcccagt ccgtccttcc
     1621 gaaaacatat ctcgcttttt tcaaaatttc gcgttcgatt cttgctcttt tcccccagat
     1681 ccggaagtca gccccgaaaa agggataacc tgatctccat tgccggatca ggccgggtct
     1741 gcttatcccg gaaaaatatt tacgggcata gaccgtaaga ttaccctgga tggaaatgat
     1801 caccgggata tcgtgcacct gtttgacaat gaggccgaat gaattttccg ttccatgaac
     1861 atgaataata tccggtttga attcttttat gattcggaga aaccgggcga ggttctcctc
     1921 atattccgcc ctgccgctta ttcggcgaag aagccttttg cttttggtga acgctgttct
     1981 tcttatggga taataatctg ttttattcaa gtggaaagga tccagttgaa ggtcgctgta
     2041 gaaaacaaag cccaggtcga cgcgtaatga attctcgact ccgctttgca gggaagaaat
     2101 ccagcctccg ccggatgttc gatcattcag aagaggctga cttaaccccg gggtattcgc
     2161 aaaccagagt acccgcaggc gcttttcgct cttttccata ttagcggcaa aaatacttta
     2221 tgatctctat tgaacgtcgc cgctcactcc cggcagaacg atattgccgg gaaaggtaag
     2281 caccgttggc gatgacatcc gtattttgcc accggtcgca gcagtccatt tgattgtgcc
     2341 gtccgaaacg gaaaaagcat aaagcttgtt atcatagctt ccggcgtata cgaccccgtt
     2401 gctgaccgtc ggactggaca tgatccggtc gccggtggcg gtgctccatt ttaccgcacc
     2461 ggtcactgca tcaaatgcat aaagcttccc gtcaaaactg cctgcataca cgaccccgtt
     2521 gctgactgcg ggactggatt gtatacggcc accggtggcc gtactccatt taattgctcc
     2581 ggataccgca ttaaacgcat aaagcttgcc gtcgaagcta cccacatagc cggttccatt
     2641 actgatcgtg ggactggaga gaacactgtc gccggtggcg aacttccact taaaggatcc
     2701 cgtaactgca tcgaaagcat atagatactt gtcggaactt ccgacataga cgatcccgtt
     2761 gctgatggcc gggctggaat tgatcgtatc cgaggtggcg gcgctccata gcttggtgcc
     2821 ggtttctgca tcaaaagcat agagactgtg atccgggctg ccaatatata tgcttccatt
     2881 actgtaaacg gggctggaaa agaagaggga agggccggaa ggctgggcgg accatttctt
     2941 tgaaccattc atattgacgc cataaacaaa tccggtataa ctcgcaatat aaatggcatc
     3001 tgtaccggtg gccgggctcg tcaggatggc gccttcggtc aggaaattcc attgcctcac
     3061 cccggtaagt gcatctatgg cgtataagcg ttggtcggta ctgccaaaat acagcacgcc
     3121 tcctttgatc gtggcgctgg attcgacatc cccattggtc gggaaagccc agacctttgc
     3181 accggtgccc gcataaaagc agtacatgtt tttatcttca ctaccgatgt aaacaaaacg
     3241 atcagcggca ggatccgggg tgagcacagc aataatattg gaagccgtcg tttccttgcc
     3301 ctggtcattc accccaacca gtttataata ataagtg
//
LOCUS       DASYCU010000321         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4565295, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000321 DASYCU010000000
VERSION     DASYCU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4565295"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..727)
                     /locus_tag="VGM24_06530"
     CDS             complement(32..727)
                     /locus_tag="VGM24_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY4155061.1"
                     /translation="MTSFSGKVISFNRQLNFSGKLPEGIRIMNPFRENKGILNLSSRF
                     YRKYYSDPGSRHLILGINPGRFGAGLTGIPFTDPKRLVEKCGIRFDGPAAHEPSSVFI
                     YEMIDAFGGAGAFYRKFYIHSVCPLGFTQSVKGGKEVNHNYYDSRELTEAVYPFILDN
                     IQKQIGLGVETDVCFCFGTGKNERFLRRVNEEKNFFKEIIALEHPRYIMQYKSKSRQA
                     YLDKYLSAFGRVL"
     gene            complement(801..1463)
                     /gene="phoU"
                     /locus_tag="VGM24_06535"
     CDS             complement(801..1463)
                     /gene="phoU"
                     /locus_tag="VGM24_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY4155062.1"
                     /translation="MSQLEIELKQLKAEVINMWNLVHSQLVKSNVALVNFDKDLAREV
                     VSREKRVNGSELKIDRDCENIFALLTPVAVDLRFVLAVLKINSNLERIGDIAEGVAKH
                     IISAEKPFNRELLKATETVSMYDEAINILEDTLSAFEGEDTALARSVFQKDEFLDKIN
                     VTATESTGKYLETHPEDIYPALYNLSIIRKLERIGDQAKNIAEEIIFYVEAKVLKHNT
                     KE"
     gene            complement(1499..2257)
                     /gene="pstB"
                     /locus_tag="VGM24_06540"
     CDS             complement(1499..2257)
                     /gene="pstB"
                     /locus_tag="VGM24_06540"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY4155063.1"
                     /translation="MERTIIKSRDFNAFFGTNHVVRQVNMDIPKGHVVAVMGPSGCGK
                     TTFLRCINRMHELIPGASSNGEMLLNNENVYQMHPIFVRLKIGMVFQRPNPFPNLSIY
                     DNVIAGYKLNGLKLPRAERDLKVEQSLTKAALWNEVKDSLHKKGTFLSGGQQQRLCIA
                     RAIAMEPEVLLLDEPTSALDPISTASIEELLHELKKNYTLVIVTHNMQQAARVSDKTA
                     FFYLGELVEYDETKKMFTNPQDKRTQNYITGRFS"
     gene            complement(2239..3084)
                     /gene="pstA"
                     /locus_tag="VGM24_06545"
     CDS             complement(2239..3084)
                     /gene="pstA"
                     /locus_tag="VGM24_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY4155064.1"
                     /translation="MNVHLKYRVAKSRLAQAVIIMLAFIITLPLLAIIIYIFREGITK
                     INWTFLTHIPKPVGETGGGIVNALLGSILIVLAASIMAVPVGILCGIYLSENRTSRLS
                     YWSRLSVDVLQGIPSIVIGIVVYFWIVKPIGGFSAFSGSIALGIMMLPILIRSTEETL
                     KLIPDSLKEAGLALGLPYHKVILKVIVPCGMSGIISGIMLSIARIAGETAPLLFTAFG
                     NPYLNTNLAKPMQSLPLLIFNYATSPYDEWHNLAWGAALILLFTVLLLNIITKLTTRR
                     WNVQL"
     gene            complement(3088..>3322)
                     /locus_tag="VGM24_06550"
     CDS             complement(3088..>3322)
                     /locus_tag="VGM24_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY4155065.1"
                     /translation="ALGETMAVTMVIGNTSLIPTSLFAPGNTMASVIANEFTEADHSV
                     YLSALIELGLVLFLVTVVINMVGKRIIKRFTNE"
BASE COUNT          890 a          814 c          745 g          873 t
ORIGIN      
        1 ttaaagatat ctcaagacgg atacagggga tttacagcac ccggccaaat gcgctaaggt
       61 atttatccag gtaggcttgc ctggatttcg acttatactg catgatatac cggggatgct
      121 caagcgcaat gatctcctta aaaaagtttt tttcttcgtt tacccggcgg agaaaccgtt
      181 catttttgcc ggtaccaaaa caaaagcaga catctgtttc aacccccagg ccgatctgct
      241 tttgaatatt atccaggata aacgggtaaa ccgcttcggt caactcccgg ctgtcataat
      301 aattgtgatt tacttccttt ccgcctttaa cggattgggt aaacccgagc ggacaaacgg
      361 aatggatata aaatttcctg taaaaagcgc cggcgccgcc aaaagcatcg atcatttcat
      421 aaatgaaaac ggaggaaggt tcgtgcgccg ccggaccgtc aaaccggatt ccgcattttt
      481 caaccagccg tttggggtct gtaaaaggaa ttcctgtaag gccggctccg aaacggcccg
      541 ggttgatccc gagtatcagg tggcgggacc cgggatcgct gtaatattta cgatagaagc
      601 gggaactgag gttaaggatc cctttatttt ccctgaacgg gttcataatg cgaatgcctt
      661 caggaagttt tcctgaaaaa ttcagctggc ggttaaagga gatcactttc ccggaaaagc
      721 ttgtcataga agggataaca aacctaaacc aaattggtta aacaaccggg agacagaaca
      781 ttcggtaata agatcgtgag ctactccttc gtattatgtt tgagcacctt ggcctcaaca
      841 taaaaaatga tctcttcggc aatatttttg gcctggtcgc ctattctttc cagtttgcgg
      901 ataatggata agttgtagag agccggataa atatcctccg gatgggtctc caggtactta
      961 ccggtagact cggtcgcagt tacgttgatc ttatccagaa actcatcttt ctgaaatacg
     1021 cttctggcga gggcggtatc ttccccttcg aatgccgaaa gggtatcttc cagtatattg
     1081 atcgcttcat cgtacatgct taccgtttca gtcgctttca gcaattccct gttaaatggc
     1141 ttttccgcac tgatgatatg cttcgccacc ccttccgcaa tatctccgat ccgctccagg
     1201 ttagaattga tctttaagac cgccagcaca aaccggagat cgaccgccac cggcgtcagc
     1261 aaggcaaaga tattttcaca gtcccggtca attttcaatt ccgacccatt gacgcgtttt
     1321 tcgcgggaca ccacttcccg ggctaggtcc ttatcgaagt tcaccagggc cacattggat
     1381 ttcaccaatt gggaatggac caggttccac atattgatca cctctgcttt taattgcttc
     1441 agttctattt ctaactggga cataaaatga aaattaaaat aaaaaataca atcagggctt
     1501 agctaaaccg gccggtaata taattctgcg ttcgtttatc ctggggattc gtgaacatct
     1561 ttttcgtttc gtcgtattcc accaattctc ccagataaaa aaacgccgtt ttatcgctca
     1621 cccgcgcagc ctgctgcata ttatgcgtta caataaccag ggtataattt tttttaagtt
     1681 cgtgcaataa ttcctcaata ctggccgtgg aaatgggatc cagtgcggaa gtgggttcat
     1741 ccagcaacag gacctcaggc tccatcgcaa tggcccgggc tatacataaa cgctgttgct
     1801 gcccgcccga aaggaaagtt ccctttttgt gcagcgaatc ctttacctca ttccatagcg
     1861 cggctttggt aagcgattgt tccactttaa gatcgcgctc ggcccgcggg agttttaacc
     1921 cattcagttt ataacccgcg atcacattgt cataaatgct gagattggga aagggattcg
     1981 gacgctgaaa taccattccg atcttgagcc ggacgaatat cggatgcatc tgataaacat
     2041 tttcattatt cagcaacatt tctccgttgc tgctggcgcc gggaataagc tcatgcatcc
     2101 ggtttataca tcgtagaaaa gtggttttac cacagccgga cggacccata accgcaacaa
     2161 catggccctt cgggatatcc atattcacct gcctgaccac atgattggta ccgaaaaagg
     2221 cattgaaatc cctgcttttt ataattgtac gttccatctt cttgttgtca gcttagtaat
     2281 gatatttaat aacagtacgg taaataataa gatcagggct gctccccagg ccaggttatg
     2341 ccactcatca taagggctgg tggcatagtt gaagatcagg aggggcaggc tttgcatggg
     2401 ctttgccaga ttggtattca ggtagggatt accaaatgcc gtaaataaca gaggagcggt
     2461 ttctcccgcg atccgcgcta tagagagcat gatgcctgaa atgattccgc tcattccgca
     2521 ggggacgatc actttcagga tcactttatg atagggaaga cccagggcca ggccggcttc
     2581 ttttagtgaa tcagggatca gtttcagcgt ttcctcggtg gaacggatca atatgggaag
     2641 catcataatg cccagcgcaa tgcttccgga aaatgcagaa aatcctccaa tgggttttac
     2701 gatccagaaa taaacgacaa tgccgatcac aatggaagga attccctgca acacatccac
     2761 cgacaaccgg ctccaatacg aaagacggct ggtcctgttc tcgcttaaat aaattccgca
     2821 aaggattcca acgggcacgg ccataatgga agccgccaga acaatcaaaa tacttccgag
     2881 caacgcattc acaatacccc ctcctgtttc tcccaccggc ttgggaatat gcgtgagaaa
     2941 ggtccagttt atttttgtga tcccttcccg gaaaatataa atgataatgg ccagcaaggg
     3001 aagcgtgata ataaaagcca gcatgatgat cacggcctgc gccagcctgc ttttggccac
     3061 ccggtatttt aagtgaacat tcatccttta ttcatttgta aagcgcttga taatgcgttt
     3121 gcccaccata ttgatgacca cggtaaccag aaacaatacc aggccgagtt caatcagggc
     3181 cgacagataa acactgtgat cagcttctgt gaattcattg gcaatcacac tggccatcgt
     3241 atttcccgga gcaaacaggc tcgtcgggat caggctggta ttgccgatga ccatggttac
     3301 cgccatggtt tcaccaagcg cc
//
LOCUS       DASYCU010000322         4873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4275218, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000322 DASYCU010000000
VERSION     DASYCU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4275218"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VGM24_06555"
     CDS             <1..368
                     /locus_tag="VGM24_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155066.1"
                     /translation="VFTACSLDDHPGNGLQDSGDTAGFINRGLDWKVMTDTSSEYRLQ
                     VSMNRSGIHYPVYVDVTPRHYQQFRVAGSVYAYDIDADGKIIAQKKLTDENGLFSFEK
                     FAITSAGGNTLLISINKKN"
     gene            397..2154
                     /locus_tag="VGM24_06560"
     CDS             397..2154
                     /locus_tag="VGM24_06560"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEY4155067.1"
                     /translation="MKKQLAFLLYTAFLSVTYAANAQSADPRNIVNGYSIYDNGYIDQ
                     PYAVVLNNGNWLCVFTTGAGTESKPGQHIVSSVSSDQGKTWTKPVDIEPSTGPSASWA
                     TPYITPFGRVYVFYDFNGDHINTMKGKPIPQNSEMGWYCYKYTDDNGNTWSDRYKLPM
                     PKAPVDFKNDFDGEVQLFWGIDKPKKTDKGMIFAFTRLGKYVQSDGEGWFYKSDNIDM
                     EKDPQQLHWSLLPEGDTGLKNPAFGSIQEEFNTVSLNNGDLFCMYRTTMGFSANAYSR
                     DGGKTWTMPVAAPYTPGGEQVFKNPRACPRVFKCTNGKYLFWYHNHGEKDFKSRNPVW
                     ISGGTEKDGLIYWSQPEILLYGLHVNDRMSYPDLILQDGKYWFTETQKTKARVHPVDA
                     ALLEGLWQQETAKKVATGGLVFQKQNIRRHKRFRMPALPGLTAGGFTADIWLEAGNLE
                     SGQVIFDSRDKKGRGIWITATANQSLRLDMSDGVLSPQGWDADPGLLSGDKAHNVIFI
                     VDGSPDIITVVVDGKLCDGGKSRVYGWGRFSEKLDNVNGGGKLILAPGRQNAIKSLRI
                     YDRYLTTSEAVSNFNAGSR"
     gene            2193..3842
                     /locus_tag="VGM24_06565"
     CDS             2193..3842
                     /locus_tag="VGM24_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY4155068.1"
                     /translation="MDIKSVKAFPAFILLVFILNTSRAQTNPRYAIIPDPAKLIARNG
                     FFNINARTTMHLESDDADMKMAVSYLVELLKNSMGFTCPMVYAGTKRNSIDFFLDSGI
                     TNEEGYSLDITTSGAIIKARTAKGIFYAVQTLRQLMPAGIETRNSGLREIPVPAVEIE
                     DTPRFVYRGVMLDVARHFFTIGFLKKYIDLLACYKINKFHLHLTDDQGWRIEIKKYPR
                     LQTISAWRKETQVGYRTDTPKRFDGIRHGGYYTQQELIDLVRYARQRFITIIPEIDIP
                     GHSQAVLAAYPQLGCIDSGYQVSRQWGVHQDILCPKEETFAFLEDVFTELIDIFPGKY
                     IHIGGDEVPKDRWKESPFCQTLIKNLGLKDEHELQSYFIGRIEKFLNSKGRAIIGWDE
                     ILEGGIAPNATIMSWHGEKGGIAAAKEKHDVIMTPSAFLYFNFYQTKNGKAAEPLSNG
                     NNFYIPLRKIYGYNPVPSVLAPDESKYILGLQGCLWTEYVASGERAENLSFPRVCALA
                     EAAWTPLSEKNYSDFVNRLNSQTAHLRRMNVNFSEFYRMDD"
     gene            complement(4074..>4873)
                     /locus_tag="VGM24_06570"
     CDS             complement(4074..>4873)
                     /locus_tag="VGM24_06570"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY4155069.1"
                     /translation="ALGITADGIDKILAKAKQRIRIENILLKEPGPQQLKMRLPAVHK
                     IIYLLFNEGYRAGSGKEIIRQELCEESLIMTKSLLDNHISDNDTAALYALLLFNAARL
                     NARITAAGELADLGEQDRTLWNDDLIALGSYYLHQSKCEKVSSYHYEAAIAYLHCHAK
                     TFAGADWASIVRLYRQLLQNNPNPFIELNYAIALYYDSQKQQSFDMLHDLQHSFLDQS
                     YLLHAALGKLYRQEGEYQKSELHLTKAISLTHFQVEKEFVRKMLIKS"
BASE COUNT         1284 a         1102 c         1240 g         1247 t
ORIGIN      
        1 cggtgttcac tgcctgctcg ctggatgatc atcccggcaa tggcctccag gattccggtg
       61 ataccgccgg gtttatcaac cgcggactgg actggaaggt catgaccgat acctcatccg
      121 aataccgctt gcaggtctcc atgaaccggt ccgggattca ctatccggtt tacgtggatg
      181 ttaccccaag acactatcag caattccggg ttgccggcag tgtgtatgct tatgatatag
      241 atgcagacgg gaaaattatc gcgcagaaaa aattgacgga tgaaaatggt ctcttcagct
      301 ttgaaaagtt tgccattacc tctgctggcg gaaatacgct gctgatcagc attaacaaaa
      361 aaaattgaaa ggacaggcca aactgaaaca ttcaacatga aaaagcaact tgcattcctc
      421 ctgtatacgg cttttttgtc cgttacttat gcggctaacg cgcaatccgc ggatcccagg
      481 aatatcgtga acggatattc catttatgac aatggttata tcgatcagcc atatgcggtt
      541 gttttgaata atggcaactg gctttgcgta ttcactaccg gcgccggaac ggaaagtaag
      601 cccggtcagc acatcgtatc ctccgtgagc agtgaccagg gaaaaacctg gacaaaaccc
      661 gtggatattg aacccagcac cggtccttcg gcttcctggg caacgccgta tatcacccct
      721 tttggaaggg tatatgtttt ttacgatttt aatggagatc atattaacac tatgaagggt
      781 aagcccattc cgcagaattc tgaaatgggc tggtattgtt acaagtatac cgacgataac
      841 ggtaatacct ggtctgatcg ctataaatta ccgatgccga aagctccggt ggattttaaa
      901 aatgattttg atggggaagt gcaactgttc tggggcatcg acaaaccgaa aaaaacggat
      961 aaaggcatga tcttcgcttt tacccgcctg ggaaaatatg ttcaaagcga tggggaaggg
     1021 tggttttaca aatcggataa tatagatatg gaaaaagatc ctcaacagct gcattggagt
     1081 cttttgcccg aaggggatac gggattaaag aaccctgctt tcggatccat acaggaggag
     1141 ttcaatacgg tgtccctgaa taatggagac cttttttgca tgtatcgcac cacgatggga
     1201 ttttcggcaa atgcatatag ccgggatgga ggaaaaacct ggacgatgcc cgtggctgct
     1261 ccttacactc cgggcggaga acaggtattc aagaatccga gagcctgtcc gcgtgttttt
     1321 aaatgtacca atggcaagta tcttttttgg taccacaatc atggagagaa ggattttaag
     1381 agcaggaatc cggtctggat cagcggcggc acagaaaaag atggtttgat ttactggtcg
     1441 cagccggaga ttctgcttta tggtctgcat gttaacgaca ggatgagcta tcccgattta
     1501 attctccagg atggtaagta ttggttcacc gaaacgcaaa aaacaaaggc acgcgtccat
     1561 ccggttgatg ccgcattgct ggaaggcctg tggcaacagg aaacggctaa aaaagtcgca
     1621 accggaggac tggtctttca aaaacaaaat atccgccggc acaaaagatt ccggatgcct
     1681 gccttgcccg gcttaacagc aggcgggttt accgcggata tctggctgga ggccggcaac
     1741 ctggaatccg ggcaggtgat attcgatagt cgggataaaa agggcagggg tatctggatt
     1801 actgccaccg caaaccaatc cctcaggctg gatatgagtg acggagtgtt gtcgccccag
     1861 ggctgggacg ctgatccggg tttgctcagc ggagataaag cgcacaatgt catttttata
     1921 gtggacggat ctccggatat tattaccgtc gttgttgatg gtaaactctg cgacgggggc
     1981 aagtcccgcg tctatggctg gggacgcttc agcgaaaagc tggataatgt gaacggcggt
     2041 ggaaaactga tcctggcgcc cggcaggcag aacgctatta aaagtctgcg gatatacgac
     2101 cggtacctga ccacgtctga agcggtcagt aatttcaatg ccggtagcag atgagactgc
     2161 tgccaaaatt ttaccgttaa acagaatgag agatggacat aaaatcggtc aaagcttttc
     2221 cggcttttat tttattggtt ttcatattga atacgtcccg ggcgcagaca aacccgcggt
     2281 atgccattat accggacccg gcgaaactca ttgcgcggaa cggcttcttc aatataaacg
     2341 caagaacaac aatgcatttg gaaagcgatg acgccgacat gaaaatggcc gtttcctatc
     2401 ttgttgaatt gctgaaaaat tcaatgggat ttacctgtcc gatggtttat gccggaacca
     2461 aaaggaatag catcgatttt tttctggaca gcgggataac aaatgaggaa gggtattctc
     2521 ttgacattac cacctcaggt gctatcataa aagcgaggac cgcaaaaggg atattttatg
     2581 ctgtccaaac attaagacaa ctgatgccgg caggaatcga aacaaggaac agcggacttc
     2641 gcgaaatacc tgttccggct gtggaaatcg aagatactcc gcgttttgtt taccgcgggg
     2701 ttatgttgga tgtggcgcgc cattttttta cgattgggtt cttgaaaaaa tatatcgatt
     2761 tgctcgcctg ttataaaata aacaaatttc atctgcacct taccgacgat cagggttggc
     2821 ggatcgagat caaaaagtac ccgcgactgc aaaccatttc tgcctggcgg aaggaaacgc
     2881 aggtgggtta ccgcaccgac acgcctaaga gattcgatgg catccgccat gggggctatt
     2941 atacgcagca ggaattgatt gacctggttc gatatgcgcg gcagcgtttt attaccatca
     3001 ttcccgaaat agatattccc ggccattcgc aggctgtttt agcggcctat ccccaactgg
     3061 gttgtatcga ctccggttac caggtgtcca ggcaatgggg tgttcaccag gatatccttt
     3121 gtccaaagga ggaaacattt gcgtttttag aagatgtgtt cactgaactg atcgatattt
     3181 tccccgggaa atatatccat atcggtgggg atgaagtgcc aaaagaccgc tggaaagaat
     3241 ctcccttttg ccagaccctg attaaaaact tggggttgaa ggatgaacac gaattgcaga
     3301 gctatttcat tggccggata gagaaatttc tgaattcaaa aggacgcgcc atcatcggat
     3361 gggatgaaat actggaaggg ggcatcgccc cgaacgcgac aattatgtcc tggcacggcg
     3421 aaaagggcgg tattgcggcg gcgaaggaaa aacatgatgt cattatgacc cccagcgcct
     3481 ttttatattt taatttttat cagactaaaa atggcaaagc agcagaaccc ctatcgaatg
     3541 ggaataattt ttatattccc ctccggaaga tttacggtta taatccggtt ccttccgttt
     3601 tggcgcctga tgaatcgaaa tatatcctgg gcctgcaggg ttgtttatgg acggaatatg
     3661 tagcttccgg agaaagggcg gagaacctgt cctttcccag ggtctgcgcc cttgccgaag
     3721 cggcctggac tcccctgtca gaaaaaaatt attccgattt tgtgaatcgg ttaaatagtc
     3781 aaacggcgca tcttcgaaga atgaatgtca acttctccga attctaccgg atggatgatt
     3841 agttttgaat aggtttttgc acagatgcca tagcccacca tccccagcca tttcaactgc
     3901 cccaaagata gggttcccga aaaaccgggt ttcgccgggt ctatatttgc tgtggcagcc
     3961 ggtattgcga tttggataat gatccttctg cgactccacc atctcaaagg aggaaaaatt
     4021 caggcccgta catgtagcgg ttccggaaca ggagccggcg gatctttgac aggtcagctt
     4081 tttatgagca tctttcttac gaattctttt tccacctgga aatgggttag tgaaatagct
     4141 ttcgtcagat gcagttcgct tttttggtat tcgccctcct gccggtataa tttgcccagg
     4201 gccgcatgga gcaaatagga ctgatccagg aagctgtgct gcaaatcgtg aagcatatca
     4261 aaggactgct gcttttgcga atcatagtat aacgcgatcg catagttcag ttcgataaag
     4321 gggttgggat tattttgcag taactgccgg tatagccgca caatgcttgc ccaatccgcc
     4381 ccggcaaagg ttttggcgtg gcaatgcaga taggcgatcg ccgcctcgta atgataggaa
     4441 gatacctttt cgcatttgga ttgatggaga tagtaactgc cgagggcaat caaatcgtcg
     4501 ttccagagcg tcctgtcctg ttctcccagg tcggccaatt cgccggctgc cgtgatccgg
     4561 gcattgagcc ttgccgcatt gaacagtaac aatgcatata aggcagccgt atcgttgtcg
     4621 gaaatatgat tgtccagtaa tgactttgtc atgatcaggc tctcttcgca tagctcctgc
     4681 cggatgatct cttttccgct accggcgcga tagccttcat tgaataggag gtagatgatc
     4741 ttgtgcacgg cgggcaatct catcttcagc tgctgtggtc ccggttcctt caaaagaatg
     4801 ttttcgatcc ttatccgttg ctttgccttg gccagtatct tgtcaatgcc atcggccgta
     4861 atacccaaag cct
//
LOCUS       DASYCU010000323         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_360155, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000323 DASYCU010000000
VERSION     DASYCU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360155"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VGM24_06575"
     CDS             complement(<1..1811)
                     /locus_tag="VGM24_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEY4155070.1"
                     /translation="MLKFVLLKPGVIESFLPIRRFLSLWLCLLVSPAFAQVSFTTVAN
                     TKDMGKTDYVQIQFKIDNAKQIDGLQPPAFPDFNVVEGPSESTGMSIINGVASQSRSI
                     SFVLQPKHAGKFTIRGATATIDGNKMSSNPVTINVRNVASYGNSQPLNNFSPLPGPAW
                     PSAEPEVDVDEVIRPGENVADKIRKNFFIKVDLSKKECYLGEPIVVTYKLYSRLHSDS
                     RVSKNPSLNGFSVYDMLDPGDDHSYVEKVNGKNFRVHVIRKAQLIALQAGDIPLDPVE
                     IDNTIYFVKVDRDQSSGTGSLLDRFFDQGPQGTPFSQHVTLDSKPVTVHVKPLPEAQK
                     PADFSGAVGRFSIQASVDSKEIDTGDAATLTVTIKGNGNLPMVNAPAVEWPADMESYD
                     VSSKEDINKASAPLGGSKKYSYSFTCSKTGKFVIPPVRLNYFDPALNAYKTVQSDPVH
                     IQINHAAKRKAAPAPLISVPTKGIAGGWLKNIFWIIGGILIGGLGIYFLMQKKRVSTP
                     VPSLVNQEPVPAEPEPLPVIDPLQESKELAGSGDYGKFYASVNRAIWKAVSDKLQLPA
                     SQLNKFNISTGLRSAGWRDEDIIRLKDLLNECEMKLYT"
     gene            complement(1889..2590)
                     /locus_tag="VGM24_06580"
     CDS             complement(1889..2590)
                     /locus_tag="VGM24_06580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4155071.1"
                     /translation="MNIVITGASRGLGYSLAEKFAVEGHDLYLTARNEVTLYHALESL
                     MARFPDQQIKARAFDLSSKEGAYAFGNWVLDLRADPDVLINNAGSFVPGMVHNEPDGA
                     LEEMLGNNLLSAYHVTRCLLPAMIKRKSGHIINMCSIASLGAYPNGGAYSISKFALSG
                     FSKNLREEMKPHHIKVTGIYPGAVYTDSWSASGVDPNRIMKPSDIADLVLQITKLSPQ
                     ALVEDLVIRPLPGDL"
BASE COUNT          649 a          739 c          707 g          679 t
ORIGIN      
        1 gtatataatt tcatttcgca ttcattcagc aggtccttga gccggatgat gtcttcgtcg
       61 cgccagccgg cggaccgcag cccggtggag atattgaatt tattcaactg gctggccggc
      121 aattgcagtt tgtcggaaac cgctttccag atggcccggt tcaccgaggc gtaaaatttc
      181 ccgtaatctc ccgacccggc cagttctttg gattcctgta atggatcgat aaccggcagg
      241 ggttccggtt cagccggaac cggttcttgg ttaaccagcg atggaaccgg tgttgacacg
      301 cgctttttct gcattaaaaa ataaatgccc agccccccga tcaatatccc cccgatgatc
      361 cagaaaatat tttttaacca gcccccggca attcctttgg tgggaacgct tatcagcgga
      421 gccggagccg ctttccgttt cgcggcatgg ttgatctgga tatggaccgg atcggattga
      481 acggttttat aggcgttcag ggccggatca aaataattca gcctgaccgg cggaatcaca
      541 aattttccgg tcttggaaca ggtgaagctg tagctgtatt tttttgaccc gccaagcggc
      601 gcgcttgcct tattgatatc ttccttactg ctgacatcgt aggattccat atccgctggc
      661 cattcaactg ccggcgcgtt caccatgggc aaattgccat tgccttttat ggtaacggtt
      721 agcgtggccg catcaccggt atcaatttcc tttgaatcca ccgaggcctg aatggaaaac
      781 cggcctacgg cgccactgaa atcggcgggc ttctgggctt cgggcagggg cttcacgtgt
      841 acggtcaccg gcttgctgtc cagggtaaca tgttgtgaaa aaggagttcc ctgcgggccc
      901 tgatcaaaaa accggtcgag caaactgccc gtgccggagg actggtccct gtccactttc
      961 acaaaataaa tcgtattgtc tatctccacg gggtccaatg gaatatcacc cgcctgcagg
     1021 gcaatcagct gggccttccg gatcacatgc acccggaaat ttttcccgtt cactttttcc
     1081 acgtaagaat gatcgtcgcc gggatccagc atatcgtaaa cgctgaaccc attcagggaa
     1141 gggtttttcg atacgcggga atctgaatga agcctggaat agagtttata ggtaaccacg
     1201 atgggctcgc cgagataaca ttccttcttg ctcagatcca cctttataaa gaaattcttc
     1261 cggatcttgt ccgccacatt ctccccgggc cggatcactt cgtccacatc cacctccggt
     1321 tccgcggaag gccaggccgg gccgggcagc ggactgaaat tattaagcgg ctgggagttc
     1381 ccgtaactgg ccacattccg gacattgatc gttacgggat tggagctcat tttatttccg
     1441 tctatcgtgg cggtcgctcc gcggatcgtg aatttgccgg cgtgtttcgg ctgcaaaaca
     1501 aaactgatgg acctgctctg gcttgcgacc ccgttgatga tgctcatgcc ggtggactca
     1561 ctcggaccct ccaccacatt aaagtcgggg aaggcaggcg gctgcaggcc gtcgatctgc
     1621 tttgcattgt cgattttgaa ctggatctgg acataatccg ttttacccat atcctttgta
     1681 ttggcaacgg ttgtaaaact cacctgcgca aaagccggac ttaccaggag gcaaagccat
     1741 aaggacagaa agcgccggat gggtaaaaaa gattctataa cgccgggttt aagtaaaaca
     1801 aacttcagca ctttgcaaat atagaaacgg agaaagggta tgcctcgaaa accaaactaa
     1861 aaatgcgtta aaacgggaat ccgccggact aaagatcgcc cggcaggggc cggatcacca
     1921 ggtcctctac cagggcctgg ggcgaaagtt tcgtgatctg caaaaccaga tctgcgatat
     1981 cggaaggttt catgatgcgg ttgggatcta ccccgcttgc ggaccaggaa tcggtataaa
     2041 ccgcacccgg atagattccc gttaccttaa tatggtgggg tttcatttcc tcccgcaggt
     2101 tttttgaaaa tcccgacagt gcaaatttgc tgatgctgta cgcaccgccg ttgggatagg
     2161 ctccgaggga ggcaatggaa cacatattaa tgatatgacc gcttttccgc tttatcatgg
     2221 ccggaagcag gcaacgcgta acatgatagg cgctcaataa attattgccc agcatttctt
     2281 ccagcgcacc atccggttca ttgtgaacca ttccgggaac aaaactcccg gcattattga
     2341 tcaggacatc cgggtccgcc cgcagatcca gcacccagtt cccaaaagcg tatgccccct
     2401 ctttggaaga gagatcaaat gccctggcct tgatctgctg atcgggaaac cgcgccatca
     2461 gggattccag cgcatggtaa agcgtgacct cattccttgc cgtcaaataa agatcatggc
     2521 cctccaccgc aaatttttca gccagactgt atccgagccc ccgcgatgct ccggttatta
     2581 caatattcat gagatgcaat ttacgccctg gccgccggtt ttgggttgat taaacaaaca
     2641 gtaaattgcg ggatgcgcta tcggcacctt ttcttcgatc tggatcatac gctctgggat
     2701 tttgaccgga acagccggga ggcgctggag gatatttata cggccctctc gctcgagctt
     2761 gccggggtga ataa
//
LOCUS       DASYCU010000324         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4276006, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000324 DASYCU010000000
VERSION     DASYCU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4276006"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VGM24_06585"
     CDS             complement(<1..561)
                     /locus_tag="VGM24_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY4155072.1"
                     /translation="MNQAEYKNVSLSEVHGTVEVNQTGPRWKQVFSFFGPAYLVSVGY
                     MDPGNWATDIAGGSKFGYTLLWVLLMSNLMALLLQSLSARLGIVRGRDLAQANRETYP
                     KTVNFCLYVLAEVAITACDLAEVLGMAIGIQLLTGLPLIYGVIITALDTFLLLSLQKL
                     GMRKIEMFILSLIAIIGLSFFLEILMA"
     gene            706..1686
                     /locus_tag="VGM24_06590"
     CDS             706..1686
                     /locus_tag="VGM24_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155073.1"
                     /translation="MGRMKKILIWAAVCSFSCCRHKPQNAGEKEKPVDIKDFMGMFTP
                     VKLPLAFGDSSLSKKPSDSSVRTSVLRQFIPDSIFIKHWGKSPKSKFYTAGKVAVRKA
                     ETYLFLKFMGTRRVLYVMGFDREGHFKAILPLVYGDEDPNISYSSVMDTRYTITLNRQ
                     RKQQFQNFYKKSVYVFNEDAAAFTLIMTESNEEKPKQAQVYNPIDTFSHKHKFTGDYV
                     QDGRNFISVRDGRSNSVIRFFVHFEKEKGSCIGELKGEARMVSPTVARYSSNGDPCSL
                     QFTFSEKNVRMKELEGCGNHRGIRCYFEGVYNKRKAAKVNRTSKKPGKRQ"
BASE COUNT          514 a          414 c          420 g          429 t
ORIGIN      
        1 tgccattaaa atttccagaa aaaaggaaag cccgatgatg gctatcaggc ttaaaataaa
       61 catttcgatt ttacgcattc ccagcttctg gagtgaaagc aataaaaaag tatccagcgc
      121 cgtaatgatc acgccgtaaa tgaggggcag gccggtcaat aactgtattc caatagccat
      181 acccaaaact tccgccaggt cgcaggcagt aatggccact tcggcaagga catataaaca
      241 gaaattaacg gtcttcggat aagtctcgcg gttggcctgc gccaggtccc ggccccgtac
      301 gatccccagc cgggcggaaa ggctctgcaa cagcagggcc attaaattgc tcatcagcaa
      361 cacccataag agagtatagc caaacttgct tcctccggcg atatcggtgg cccagtttcc
      421 cgggtccata taccccacgc tgaccagata ggcgggacca aaaaaagaaa aaacctgctt
      481 ccagcgggga ccggtttgat tgacttccac cgtaccatgt acttcactaa gagagacgtt
      541 tttgtattca gcctggttca tgaatttgat ccggtatttt cggtcagctg cattgtggga
      601 agccccggtt tgaacaaatc taaaatatgt tccgaacagt tgcggaaaat attaccgggg
      661 ctcagttatt gccgaatctt tatttttgat tttaaataag ggttcatggg acgaatgaaa
      721 aagatcctga tatgggcggc cgtttgctca ttttcctgct gccggcacaa accgcaaaat
      781 gcgggagaaa aagaaaaacc cgttgatatc aaggatttta tggggatgtt taccccggtt
      841 aagctgccgc tcgcctttgg agacagctcc ctttccaaaa aaccttccga ttcatccgtc
      901 aggacctccg ttctgaggca gtttatcccg gactccattt ttataaaaca ctgggggaag
      961 agcccgaaat caaaattcta tactgctggc aaagtggcgg ttagaaaggc agagacctat
     1021 cttttcctca aatttatggg aaccaggaga gtgctgtacg tgatgggatt cgacagggaa
     1081 ggccatttca aggcgatctt gccgctggtg tacggagacg aggatccgaa tatcagctat
     1141 tcttctgtaa tggataccag gtataccata acgctgaacc gccagcgaaa acagcagttc
     1201 cagaattttt ataagaaatc agtctatgtt tttaatgaag acgccgctgc gtttaccctc
     1261 atcatgacgg aatccaatga ggaaaaaccg aagcaggccc aggtctataa tcccatcgac
     1321 acgttttcac acaaacataa attcacggga gattatgtcc aggacgggcg gaattttatc
     1381 tctgtccgcg atggcaggag caattcagtg atccgttttt ttgtccattt tgaaaaggaa
     1441 aagggatcct gtattggtga attgaaagga gaggcccgga tggtttcccc taccgtggcc
     1501 cgctacagtt ccaatgggga tccctgttca cttcaattca cgttcagtga gaaaaatgtg
     1561 cgcatgaaag aactggaagg ctgtgggaat caccggggta tccgttgtta tttcgagggg
     1621 gtctacaata agcgcaaggc ggccaaggtt aaccggacca gcaaaaagcc aggaaaaaga
     1681 caataaaata aaataatttc gcagccgttt acgcaatcaa tatgagtttt acacctttta
     1741 agcatccata ccccatcgac gaccggtatt ccaagaa
//
LOCUS       DASYCU010000325         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4421291, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000325 DASYCU010000000
VERSION     DASYCU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4421291"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2175
                     /locus_tag="VGM24_06595"
     CDS             <1..2175
                     /locus_tag="VGM24_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY4155074.1"
                     /translation="ETLIRELSADKQGFNSVYMMLDSGARGSKQQIKQLAGIRGLMAK
                     PRKSGSTGSEIIENPILSNFKGGLNVLDYFISTHGARKGLADTALKTADAGYLTRRLV
                     DVAQDVVIKDEDCGTLRGIATTALKDNEDIVEPLYDRILGRVSLHDVYDPTTDQIIAK
                     GGVEITEEVAKKIEEAGIETIEIRSVLTCESKRGVCVKCYGKNLATGYTAQKGDAVGI
                     IAAQSIGEPGTQLTLRTFHVGGVAGSASVESSLLAKFDGTIQFDGLRTVLTENNEGAK
                     VPIVIGRTGEVRIMDVKNDRLLITNNVPYGATLAVKDGQKVAKGDVICTWDPFNNVVI
                     TEITGKLKFENVLEGITYREEADEQTGHREKVVIETKDKTKIPSIIVEGGKDIKSYNL
                     PVGSHIILDTGEEVRAGQVVVKIPRVLGKLRDITGGLPRVTELFEARNPGNPAVVSEI
                     DGVVAFGNIKRGNREIIVEAKDGIVKKYLVPLTRQILAQDGDFIKAGAQLSDGQIAPA
                     DILSIKGPFAVQEYVVNEIQEVYRLQGVKINDKHIEVIVRQMMRKLNIVDPGDTRFLE
                     DETVDKIEFLDENDWIFDKKVVTEAGESTKLKPGQIVSLREVREENSILRRNDKKLAE
                     FRDAHSATSAPMLLGITKASLGTQSWISAASFQETTKVLSSAAIQGKTDDMMGLKENV
                     ITGHPIPAGTGLREFENMIVGSKEEYELLQTSREAMQFDEEE"
     gene            2283..2870
                     /locus_tag="VGM24_06600"
     CDS             2283..2870
                     /locus_tag="VGM24_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY4155075.1"
                     /translation="MRNIAMIPGFLALLLTGVLFYVIYKRPADVQPVRSGSAGNAQAP
                     FRIAYFNLDSLEAHYEYFKDVLDQVKGKENDMNAQLSNMEKGYQKKIAEWQKKGNSMS
                     AAESEQAQQEYAQMQQAYQIKKQTLQESLLKHNEDLKADIRKKIEDYLAEYNKDKGYN
                     FIISYESNSFIYVKDTTYNITGELIQGLNALYKKK"
     gene            2930..4402
                     /locus_tag="VGM24_06605"
     CDS             2930..4402
                     /locus_tag="VGM24_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4155076.1"
                     /translation="MSGESPSFRPSLGLMDATMIVAGSMIGSGIFIVSADIVRNVGSA
                     GWLILVWLITGFMTITAAVSYGELSGMFPRAGGQYVYLKEAYNPLIGFLYGWSFFSVI
                     QTGTIAAVGVAFSKYAAYLIPALSEDNRLISIPTGVNACGDPSFFSISAAQVTSIGII
                     ALLTYTNTRGIQGGKRIQNIFTSTKLVALFGLILAGFIAFHPEVWHANWSHAWESTKT
                     ALRDVCHPSSGFTVTPISGAALFGGIAAAMVGSVFSSDAWNNVTFIAGEIRNPQKNIG
                     ASLFFGTLIVTLIYIGANLMYISVLPLDGLAHAEKDRIAVSAAHVIFGNGGTIIIALL
                     IMVATFGCNNGLILSGARVYYTMAKDKLFFQKAGTLNKNAVPQWALWAQFVVASILCL
                     SGKYGDLLDMISFVVVIFYVLTIAGIFILRKKRPESPRPYKAFGYPVLPALYILMGIS
                     FCILLIIYKPEFTWPGLIITLLGIPLYFIAGSGKRKTTEP"
BASE COUNT         1166 a         1062 c         1177 g         1057 t
ORIGIN      
        1 gaaaccctga tccgtgagct cagtgccgac aagcagggat tcaattccgt atacatgatg
       61 ctggattccg gagcccgggg ttcaaaacaa cagatcaagc agcttgccgg catacgcgga
      121 ttgatggcca agcccaggaa atccggatcc accggaagtg agatcattga aaatcccatt
      181 ctgtccaatt tcaaaggcgg attgaacgta ctggattatt tcatctccac ccatggtgcc
      241 cggaaaggtc ttgctgatac cgcgttgaag acagcggatg ccggttacct cacccgccgt
      301 ttggtggacg tggcgcagga tgtggttatc aaggacgaag attgcggaac cttacgcggt
      361 atcgcaacca cggcgctgaa agacaatgaa gatatcgttg aacctttata cgacaggata
      421 ctcggacgtg tttcccttca tgatgtatat gatccgacca cagaccagat cattgcaaaa
      481 ggtggagttg aaatcacaga ggaagtggcc aaaaaaattg aagaggccgg aattgaaacc
      541 atcgagatcc gttccgtgct cacctgcgaa agcaagagag gggtttgcgt aaaatgttat
      601 ggtaagaatc tcgccaccgg atataccgcg cagaagggag acgccgtagg gattattgcg
      661 gcgcagtcca tcggcgaacc gggtacccag ctcaccctgc ggaccttcca tgtgggtggt
      721 gttgcgggat cggcatccgt tgaatcttcg ctcctggcaa aatttgacgg taccattcag
      781 ttcgacggat tgagaaccgt actgaccgaa aataatgaag gagctaaagt cccgatcgtc
      841 atcggccgta ccggagaagt ccggatcatg gatgtgaaga acgaccgtct gctgattacc
      901 aataacgttc cctatggcgc cacgctggcc gtgaaagacg gacagaaagt ggccaagggc
      961 gatgtgatct gcacctggga tccgttcaac aatgttgtta ttacggaaat taccggtaag
     1021 ctcaaatttg aaaatgtact ggaaggaatc acctaccggg aagaagcgga cgaacaaacc
     1081 ggtcaccggg aaaaagtggt tattgaaaca aaggacaaaa ccaagattcc atccattatc
     1141 gtggaaggag gaaaggatat taaatcctac aacctgcccg tgggcagtca tatcatattg
     1201 gatacgggtg aagaggtccg tgccggtcag gtggtcgtaa agatcccgcg tgttctcgga
     1261 aaactgaggg atatcaccgg cggtctcccc cgggtaacgg aattattcga agcgcgtaat
     1321 ccgggaaatc cggccgtggt ttctgaaata gacggggtcg tcgccttcgg aaatatcaag
     1381 cggggcaacc gggagatcat cgtggaggcc aaggacggta tcgtgaaaaa gtacctggtg
     1441 ccgctgaccc gccagatcct ggcgcaggac ggcgacttca tcaaggccgg cgcccagttg
     1501 tcggatggac agatcgcacc ggcggatatt ctttccatca aaggcccgtt tgcggtgcag
     1561 gaatatgtgg tgaatgaaat tcaggaagta taccgcctgc agggggtaaa gatcaatgac
     1621 aagcacattg aagtgatcgt tcgccagatg atgcgtaaac tgaatattgt agatcccgga
     1681 gacacccgtt tcctcgaaga tgaaacggtg gataagatcg agttcctgga tgagaatgac
     1741 tggatctttg ataagaaagt ggttacggaa gccggtgaat cgaccaagtt aaaacccggg
     1801 cagattgtca gcctccggga agtaagggaa gaaaattcca ttctgcggag aaacgataaa
     1861 aaactggcgg aattccggga tgcccattcg gctacatcgg cccccatgct gctgggtatt
     1921 accaaggcct ccctgggaac gcaaagctgg atttcagctg cttctttcca ggaaaccacg
     1981 aaagtgcttt catcggccgc catccagggt aaaaccgacg atatgatggg gttgaaggaa
     2041 aacgtgatca cgggtcatcc gatcccggca ggtaccggct tgcgcgagtt tgagaacatg
     2101 atcgtaggca gtaaggaaga gtatgaactg ctgcaaacgt cccgggaagc catgcagttt
     2161 gatgaagagg aataggatca aaatttaaga attaataagc agtaggaagc agaaatgctt
     2221 cctactttta tgtcgggtag tactggtccg gaacggatca ttcatttaaa tcgttgttgt
     2281 ctatgagaaa tattgcaatg attccgggct ttcttgccct gctgcttacc ggcgttttat
     2341 tttatgtcat ttacaaacgc ccggcagatg tccagcccgt acgttccggt tcagcgggaa
     2401 atgcccaggc gcccttccgg attgcctatt tcaacctgga ttccctcgaa gcgcattacg
     2461 aatatttcaa ggacgtgctg gatcaggtta agggtaagga aaatgatatg aatgcccaac
     2521 tcagcaacat ggagaaaggg tatcagaaga agatcgcgga atggcagaaa aaaggaaatt
     2581 cgatgtcggc ggctgaaagc gaacaggccc agcaggaata tgcccagatg cagcaggctt
     2641 accagataaa aaaacagacc ctccaggaat ccctcctgaa gcacaatgaa gacctgaagg
     2701 cagatatccg gaaaaaaatt gaagattatc tggccgaata caataaggat aagggctata
     2761 attttattat ttcttatgag tccaattcct ttatttatgt aaaggatacc acgtacaata
     2821 tcaccggtga actcatccaa gggctgaatg ccctctacaa gaaaaagtag gcggcccgcc
     2881 ctacgcccgt tttgcttccg atttactatc ttccggttta aatcagacta tgtccggaga
     2941 aagcccttcg tttagaccca gcctcggatt gatggatgct accatgatcg tcgcgggctc
     3001 catgatcggc tcgggcatct ttatcgtatc cgccgatatt gttcgtaatg tgggaagtgc
     3061 cggatggctg atcctggttt ggctgatcac gggttttatg acgatcaccg ccgcggtgag
     3121 ttatggcgag ctgagcggta tgtttccccg ggccggcggg caatatgtct accttaaaga
     3181 agcctacaat cccctgatcg gattccttta cggctggagt tttttttccg tgatccaaac
     3241 agggaccatc gcggcagtag gcgttgcctt ttccaaatac gcggcttacc tgatcccggc
     3301 actcagcgaa gacaaccggc tgatcagtat tccgaccggc gtgaatgcct gcggagatcc
     3361 gtcttttttc agtatcagtg cggcccaggt aacctccatc ggcatcattg ccttactaac
     3421 gtatacgaat acgcgcggta ttcagggggg taaaaggatt cagaatattt tcacttccac
     3481 caaactggtt gcattattcg ggctgatcct ggccggtttt attgcttttc atccggaagt
     3541 atggcatgcc aactggtccc atgcctggga atccaccaaa accgcgttgc gggacgtctg
     3601 tcatccttcc tccggattca ccgtcacgcc catcagcgga gccgccctgt tcggcggaat
     3661 cgcggccgcg atggtgggtt ccgtcttcag cagcgatgcc tggaacaacg tgacgtttat
     3721 agccggagaa atcaggaacc ctcaaaaaaa tatcggcgct tccctgtttt tcggaaccct
     3781 gatcgtaacg ctgatctata tcggcgccaa cctgatgtat atttcagtac ttccgctgga
     3841 cgggctcgcg catgccgaaa aagaccgcat tgccgtatcg gccgctcatg tcattttcgg
     3901 aaacggggga acgatcatca ttgccctgct cattatggtg gccaccttcg gctgtaataa
     3961 cggtttgatc ctttccgggg cccgggtgta ttataccatg gcaaaagaca aattgttctt
     4021 ccaaaaggcc gggacgctga ataaaaatgc ggttccccaa tgggcgctct gggcccagtt
     4081 cgtggttgct tccattttat gcctcagtgg taaatatgga gatctcctgg atatgatttc
     4141 ctttgtggtc gtgatatttt atgtactcac cattgcaggc atattcattc taagaaagaa
     4201 aaggcccgaa tcgccgagac cgtataaggc cttcggatat ccggttctgc cggccctgta
     4261 tattctcatg ggcatcagtt tttgcatcct tctgattatt tataaaccgg aatttacctg
     4321 gcccggattg atcattaccc tgctggggat acccctgtat tttattgctg gatcgggtaa
     4381 gcggaaaacg acggaacctt aatatgtcat cgatcgattt taaacagatt ttcaaaagct
     4441 tcacttcggt aaagatcggc gt
//
LOCUS       DASYCU010000326         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2536013, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000326 DASYCU010000000
VERSION     DASYCU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2536013"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /gene="ligD"
                     /locus_tag="VGM24_06610"
     CDS             <1..1642
                     /gene="ligD"
                     /locus_tag="VGM24_06610"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY4155077.1"
                     /translation="FSDHVEEDGIAFFDAAISKGLEGVMAKKADSLYAENRRSQNWLK
                     IKNNQFAEAIICGFTKGRNSRKYFGAIILGRYKGKELIYSGHSGSGIDEKGLKELSAK
                     FQPLITEKCPFKVKPKTNMPATWLMPELVCMVKYTEITKDGSLRHPIFAGLREDKNGA
                     DEKNIRVLKIPGKGPERKNATKKKSPGKKSVKVEKAGTPSMRKAPAGKAKANSKLKSA
                     EEPAGKARGKKDSDNPGGQLVDPAEKQAEVTIRKHTLSLSNLPKIYWPGEKISKGDMI
                     NYYYQVAGFILPYLKDRPQSLNRYPNGIEGESFYQKNIGEQLPEWIDTYQYKSESDGK
                     IKHYLVVKDEASLIYMANLGCIEMHPWHSRIQKPDYPDWCVIDIDPDKTNTFNQVIDV
                     ALQIRAHLHQLKIESYCKTSGSTGMHIYIPLGAKYTYDQSRMFAEMLVTGVQQEIPAF
                     TSIERSLSKRKGKIYLDFLQNRQIQTIASPYSLRPKPGAPVSMPLYWEEVKHGLKILD
                     FNINNSLERIRRESEHFKAVLGPGINLGKILSEMGKGS"
     gene            1672..2049
                     /locus_tag="VGM24_06615"
     CDS             1672..2049
                     /locus_tag="VGM24_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY4155078.1"
                     /translation="MKKINISIKRVYAPPDEKDGTRILVDRLWPRGLTRERAKVDLWL
                     KEIAPSTNLRKWFGHEPDKWTGFKERYIRELNINKESVSILKERLKKGPVTLVYGAKD
                     ELHNDAVCIKEWLSHSSLEQSLP"
     gene            2095..2463
                     /locus_tag="VGM24_06620"
     CDS             2095..2463
                     /locus_tag="VGM24_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155079.1"
                     /translation="MRQRSISSGTFISATICLFILVTGFNLPRNRFKENLLPEGGSIL
                     GIVVPFSVKTTVLAVSVSDTIASTTSDTTNGAYILKDIPPGNYALLYVPSDNQYRKNS
                     TRAVVSEGQITVADTIWLRK"
     gene            complement(2506..>3017)
                     /locus_tag="VGM24_06625"
     CDS             complement(2506..>3017)
                     /locus_tag="VGM24_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155080.1"
                     /translation="ITGQHFDPVASLVRIGGQLVAGFTYSDLGNGSQELVKQNFTPAS
                     NLNNPATITVSVSNVNSNAYSFLFYPEIKSITPDTVYENENVSLQGILFGDRTLTSTV
                     KAFYFDQGQNKIYMAPDPAILSWNTNTLHVSMPDYGSYPIGSGDSPFYLEVTVGSKSG
                     SAPLYFHIL"
BASE COUNT          893 a          688 c          754 g          682 t
ORIGIN      
        1 attcagcgat catgtggaag aggacgggat tgcctttttt gatgcagcca tatccaaggg
       61 actcgaaggg gtcatggcca aaaaagcgga cagcctgtat gctgaaaacc ggcgcagcca
      121 aaactggttg aagatcaaaa acaatcagtt tgcagaagca ataatctgcg gattcaccaa
      181 gggccggaac agccggaaat atttcggggc gattatcctt ggccgttata aaggaaagga
      241 attgatatat tccggtcaca gcggttcggg aattgatgaa aaaggcctga aggaactttc
      301 tgcaaaattt cagccgctga ttacggaaaa atgccctttc aaagtaaaac caaaaaccaa
      361 tatgccggcc acctggctga tgcctgaact ggtttgcatg gtcaagtata ccgaaataac
      421 caaggacggg agcctgcgtc atcccatatt tgcgggtttg cgggaagata agaacggcgc
      481 cgatgaaaag aatatccggg tgttgaaaat tccaggtaag ggcccggaga gaaagaatgc
      541 aactaaaaag aaatcaccgg gtaagaaaag cgtaaaagtt gaaaaggccg gaacgccgtc
      601 catgagaaaa gcacctgcag ggaaggcgaa agcaaacagc aaattgaaat cggcggagga
      661 accggcaggt aaggcccggg ggaaaaagga ttctgacaat ccgggtggcc agttggtaga
      721 tccggccgaa aaacaggctg aagtaacgat ccggaaacat accctttccc tgtccaatct
      781 gccaaaaatt tattggccgg gggaaaagat cagcaagggg gacatgatca attattatta
      841 ccaggtagcc ggattcattc tcccctatct gaaagacagg ccccagtccc tcaaccggta
      901 tccgaatgga attgaaggcg agagctttta ccagaagaat atcggcgaac agttgcccga
      961 atggatagat acctaccaat ataaaagtga gtccgatgga aagatcaagc attacctggt
     1021 ggttaaggac gaggcgagcc tgatctatat ggcgaacctg ggttgcatcg aaatgcatcc
     1081 ctggcatagc cggatacaaa agccggatta cccggactgg tgcgtaattg atatcgatcc
     1141 cgataaaacc aacacgttta atcaggtgat tgatgtggct ctgcagatca gagctcatct
     1201 gcatcaactc aaaatagaat cgtattgcaa aacttccgga tcgaccggga tgcatattta
     1261 tattccgctt ggcgcaaaat atacctatga ccagtcacgg atgttcgccg aaatgctggt
     1321 gaccggggtt cagcaggaaa tacccgcctt caccagtatt gagcgcagcc tgtccaaaag
     1381 aaaagggaaa atctaccttg actttctgca gaaccgtcaa atacaaacga ttgcgtcccc
     1441 ttattccctg cgtcccaaac cgggcgcgcc ggtgtccatg cccctgtatt gggaagaagt
     1501 aaaacatggt cttaagatcc tggacttcaa tatcaataat tcactcgagc gtatccgccg
     1561 ggaatcggaa cattttaagg cggtgctcgg gcccggaata aacctgggaa aaatattatc
     1621 tgaaatggga aaaggcagtt aagcgggaac aacctcaaac gatcgtcgcc aatgaaaaaa
     1681 ataaatataa gcattaaaag agtatatgcc cctccggacg aaaaagacgg cacccgcatt
     1741 ttggtagaca ggctctggcc gagaggcctg accagggaaa gagccaaggt ggacttatgg
     1801 ctgaaagaga tagcgccgag cacaaacctc cgtaaatggt tcggtcatga gccggataaa
     1861 tggaccggct ttaaagagcg gtatatccgg gaactgaata taaataagga atcggtatct
     1921 attctgaaag aacggctaaa aaagggtcct gttacgctgg tttacggagc aaaggacgaa
     1981 ctgcacaacg atgccgtttg tattaaggaa tggctgtctc attcttctct ggaacaatct
     2041 ttgccttaga taaccggata tcggattgcc agcggctttt taactcaatt cctgatgagg
     2101 caaagaagta tttcttccgg aacgtttatt tctgcgacca tctgcttatt catcctggtc
     2161 acggggttta accttccgcg aaaccgattt aaagaaaacc tgcttccgga agggggttcc
     2221 atactcggca tcgttgtgcc attttcagtt aaaacgactg ttctggccgt ttctgtttcc
     2281 gacacaatag ccagcaccac ttcggacacg acgaacgggg catatatctt aaaggatatt
     2341 cctcccggca attacgcttt gctttatgtt ccttcggata atcaataccg aaaaaattcc
     2401 acccgcgcgg ttgtttctga aggacagatt accgtagcgg atacgatctg gctgaggaaa
     2461 taagacattg cttcttaccc gcggcagatg caagccgggt ttgttctaca ggatatgaaa
     2521 atacaagggg gcactaccgc tctttgaacc caccgtcact tccaggtaaa acggagaatc
     2581 cccggatcca atcggataac tgccatagtc cggcatgctg acgtgaaggg tatttgtatt
     2641 ccaggacagg atggccgggt cgggcgccat atagatttta ttctgtccct gatcaaaata
     2701 aaatgcttta accgtgctgg tcagcgtccg atctccaaac aggatcccct gcaggctcac
     2761 attttcgttt tcgtaaaccg tatccggagt gatggatttg atttcaggat aaaataaaaa
     2821 cgagtacgca ttgctgttaa cattgctgac ggataccgta attgttgccg gattgttcag
     2881 gtttgaagcc ggcgtgaaat tctgtttaac gagttcctgc gatccgttgc ccaaatccga
     2941 atacgtgaag ccggcaacca gctgcccgcc aatcctgact agggacgcca cgggatcaaa
     3001 atgctgaccg gttatgc
//
LOCUS       DASYCU010000327         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4421751, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000327 DASYCU010000000
VERSION     DASYCU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4421751"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1916)
                     /locus_tag="VGM24_06630"
     CDS             complement(237..1916)
                     /locus_tag="VGM24_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4155081.1"
                     /translation="MADNVYDAIVIGSGISGGWAAKELCEKGLKTIMLERGRNIEHVK
                     DYVNANKEAWDYPHRGTRTQQMIKDYPVLKRDYPLNETNLDYWCSDKDSPYTEIKRFD
                     WFRGYHVGGRSLMWGRQSYRWSDHDYEANAKDGHGVDWPVRYKEIAPWYDHAERFAGI
                     SGNRDGLEVLPDGQFMPAMEMNCVEKDFVGRMKDHFKGKRPVIIGRTANITEPLPGRT
                     NCQYRNKCWLGCPFGAYFSTQSSTLPAAMKTGNLTVRPWAIVTKILYDKDKKRATGVE
                     ILDGETNKTYTYQAKIIFLNASTLNSAWILMNSATDVWPDGLGSSSGELGHNIMDHHL
                     GVGASGMVDGFDDKYYYGRRANGIYIPRFRNIFGDKREYVRGFGYQGGGSREGYRRSP
                     EEVAIGLDLKNALVEPGPWSLGIGGFGETLPYHENKVTLDKTRKDKWGLNILAMDIEY
                     KDNEVKMRKDMKDDAVEMLTAAGLKNVRGHEGDGTLGRGIHEMGSARMGKDPKTSVLN
                     KYNQIWDAPNVFVTDGSFMVSSSCVNPSLSYMAFTARAADHAVSELKKHNI"
     gene            complement(1939..2790)
                     /locus_tag="VGM24_06635"
     CDS             complement(1939..2790)
                     /locus_tag="VGM24_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY4155082.1"
                     /translation="MIAGSAALVTGGPILSSYSPHPKSDTVDLKGNINHSVCRWCYES
                     IPYEDFCRQVKDLGLKAIDLVAPKDWPLLQKYGLYSSMCYVAGEVSLTKGFADPANHP
                     TLIAQYLDVFPQMVSAGYKDVICFSGSRAGMDDETGWTNCVKGLKEIMPQAEKQGITV
                     HMELLNSKVDHKDYMCDHTNWGVTLCKRLASPNFKLLYDIYHMQIDEGDVIRTIEDNY
                     EYLGHYHTGGVPGRHEIDETQELYYPAIMRAILKTGFRGYVAQEFIPASEDKIGSLGK
                     AIQICDI"
     gene            complement(2879..>3113)
                     /locus_tag="VGM24_06640"
     CDS             complement(2879..>3113)
                     /locus_tag="VGM24_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155083.1"
                     /translation="LMIRKLPAGADLDSYRSAAAGTEMSRLFDGARSAAGPEVLNTTL
                     IIPVILIVAFAGLNIYMRGRKKPDILVASPAGH"
BASE COUNT          662 a          876 c          746 g          829 t
ORIGIN      
        1 ggtctctccc acctcatcca ggaaagcaat atcatcggga gaaaactgca aaccggccgt
       61 gccgatcttt ttatccgcat tggtacagcc ggataaaaac atgtcagctc ctatgatcgt
      121 accgcccagc agcagggcta cggacgataa ggcttcgcgt ctgttcattt gttttaattt
      181 gaaatttaaa aaatttaaga atctgaattt acaccgtccc ggattcccgg ttgatcctaa
      241 atattatgtt tctttaattc actcaccgca tgatcggcgg cccttgccgt aaatgccata
      301 taagaaagcg aggggttcac gcaggaagac gataccatga acgatccgtc cgttacaaat
      361 acattcgggg catcccagat ctggttgtat ttgttcagca cggaagtttt cgggtccttc
      421 cccatccggg cggatcccat ttcatgaata ccccggccca gggtgccgtc gccctcatga
      481 ccgcgcacat tcttgagacc cgcagcggta agcatttcca ccgcatcgtc tttcatatcc
      541 tttctcattt tcacttcgtt gtccttgtat tcgatatcca tcgccaggat attcagtccc
      601 cacttgtctt tccgggtttt atccagcgtc actttatttt cgtgataggg aagagtttcg
      661 ccgaagccgc cgattcccag gctccagggc cccggttcca ccaatgcatt tttcaggtcc
      721 aggccgatgg cgacttcttc cggggaacgc cggtaacctt cgcggcttcc tccgccctgg
      781 taaccgaatc cgcggacata ttcgcgctta tcgccgaaaa tattccggaa gcgcggaata
      841 taaattccgt tggcccttct tccataataa tatttgtcat caaatccatc taccattccc
      901 gaagcgccca cccccaggtg atggtccatg atattatgac cgagttcccc gctgctgctg
      961 cccaggccgt cgggccatac atccgttgcg gaattcatca ggatccacgc gctgttcagg
     1021 gtggatgcat tgaggaaaat gatctttgcc tggtaggtat aggtcttgtt tgtttccccg
     1081 tccaggatct ccacgcccgt cgctcttttc ttatccttgt cgtaaagaat ttttgttacg
     1141 attgcccatg gccgcacggt gagattgccg gtcttcatgg ccgccggcag ggtcgacgac
     1201 tgcgtgctga aataagcgcc gaagggacaa cccagccagc atttattccg gtactggcaa
     1261 ttggttctgc cgggaagggg ttcggtgata ttggccgtgc ggccgatgat gaccgggcgc
     1321 ttgcctttga aatgatcttt catccggccc acaaaatcct tttccacgca gttcatctcc
     1381 atggcgggca tgaattgtcc gtcgggtaga acctccagtc cgtcgcggtt tccgctgatc
     1441 cccgcaaacc gttctgcgtg atcgtaccag ggcgcaattt ctttataccg caccggccag
     1501 tcgaccccgt gcccgtcctt cgcgttggct tcgtagtcgt gatcgctcca gcgatagctt
     1561 tgacgtcccc acatgaggga acggccgccc acatgataac cccggaacca gtcgaaccgt
     1621 ttgatctcgg tataggggct gtccttatcg ctgcaccagt aatcgaggtt ggtttcattc
     1681 agcggataat cccgtttcag caccgggtaa tccttgatca tctgctgcgt tcgggttccg
     1741 cggtgcggat aatcccatgc ttccttgttt gcattcacat aatctttgac gtgttcgata
     1801 ttccggccac gttccagcat gatcgttttc aatccttttt cacaaagttc ttttgcggcc
     1861 catcctccgc tgatcccgga tccgattaca attgcgtcat aaacattgtc tgccatgata
     1921 agagctgttt aatttttatt atatatcaca aatctgaatt gcttttccca gggacccgat
     1981 cttgtcttcc gaagcgggga tgaactcctg cgcaacatat cccctgaaac ccgtcttcag
     2041 gatggcccgc atgatggccg gataataaag ctcctgggtt tcgtctatct cgtgccggcc
     2101 cggcacgccg ccggtatggt aatgtcccag atattcgtaa ttgtcttcga tggtccggat
     2161 cacatctccc tcatcgatct gcatgtggta gatatcgtat aataatttaa aattgggact
     2221 ggcaagtctt ttacacaggg tgacccccca gttcgtatgg tcgcacatgt aatccttatg
     2281 atccactttg ctgttgagca gctccatatg cacggtgatg ccctgttttt cagcctgcgg
     2341 cataatttct ttcaatcctt ttacacagtt ggtccagccc gtttcatcgt ccatgccggc
     2401 gcggcttccg ctaaaacaga tcacatcctt gtaaccggcg ctcaccatct ggggaaacac
     2461 atccaggtac tgggcgatca gcgtggggtg attggcggga tcggcaaatc ccttggtcag
     2521 gctcacctcc ccggccacat agcacatgga actgtagagt ccgtatttct gcaacagggg
     2581 ccagtctttc ggcgccacca ggtcaatggc tttcagtccg aggtccttca cctgccggca
     2641 aaaatcttca tacggaatgc tctcgtaaca ccagcggcat acggaatggt tgatatttcc
     2701 tttgaggtcc accgtatctg atttgggatg aggagaatag gaagacagta tgggtccccc
     2761 tgtaactaat gcggctgagc cggcaatcat attgcggata acttttcggc gggatgatgg
     2821 atgatccatg gcaatcaagg tggttaatgt acgaaattct gcactataaa aagccgtctt
     2881 aatgaccagc gggtgaagcc accaggatat cgggcttttt tctgccccgc atatagatgt
     2941 tcaatccggc aaaagcaacg atgaggatca ccggaatgat cagggtggtg ttcaacacct
     3001 cggggcccgc ggccgacctg gccccatcaa aaaggcggct catttccgtt cccgccgccg
     3061 ccgaacgata gctgtccagg tcggcccctg ccggcagctt tctgatcatc agc
//
LOCUS       DASYCU010000328         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_361149, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000328 DASYCU010000000
VERSION     DASYCU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361149"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VGM24_06645"
     CDS             <1..248
                     /locus_tag="VGM24_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155084.1"
                     /translation="LDSKGRGMVTGYDVAVECGGVLVNPGDLIYSDFDGIVVVPQGIE
                     DEVFQRAKEKVENENHSRRELLDGRSLRQVYDKYKSL"
     gene            489..>2805
                     /locus_tag="VGM24_06650"
     CDS             489..>2805
                     /locus_tag="VGM24_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4155085.1"
                     /translation="MNLHSRTLRNWQKRVLLQALFVFFALFSFAQKITLSGPVTDGKA
                     PLEKVSIRVLGSGRGTTTNENGEFKISAVKGQTLVFSYLGFEEKRVLVGDQTEIAVSL
                     TASAQDALNDVVVVGYGTKRKLNVTGALSTVSAKQLENRPVTNVADALQGTMAGVTVV
                     QNSGQPGKDQGTIRVRGIGTLGNSDAMVVVDGIVSTLNDINPNDIESITVLKDAASAS
                     IYGSRAANGVILVTTKKGKNGATVVHYNAYVGKQQPTRLPDYLPSWQASSFYNEALVN
                     EGKTPRYSDAEIQKFKDGSDPDNYPNTDWLGLFYKGSGFQQSHYVDVSGGNEKTQSLL
                     SLGYFSEDGIVKNTGLDRYTTRFKISTRLGSRFSVNGNFAYTLENFKEPVDGQNRGFQ
                     ALIHGINRIGNVVPYKYSNGYYGFNDEGNPVASVDEGGLAQTNTHYLKGVVDGDLEII
                     KGLHFKPLLGYQLTIDQSKNFIKDMQFYDWQTGAPALYLGPNSLTNTNNITSVVTLQG
                     LLQYDKNWGDHDFSVLAGYSQEYTHFSNLMGYRKDFLNNSLSELNAGPVAGQQATGSA
                     YEIALQSFFGRINYSYKGKYLLEGNIREDGSSRFASDNRWGVYPSFSAGWRISEEGFF
                     SPLKTLLNDLKFRGSWGKLGNQNLSTIQNSSGSPIGNYPYISTISSGQNYTFGDAVAS
                     GVAPLNGANAMIQWESTTSTDFGFDASFLQGKITLTADYFIRNTDNLLLNIPVGAVYG
                     FNGPVQNGGSIQNKGLELELGYHGRQKDFTYN"
BASE COUNT          760 a          672 c          736 g          637 t
ORIGIN      
        1 ccctggattc aaaggggcgg ggcatggtga cgggctatga cgtggcggtg gaatgcgggg
       61 gagtgctggt gaacccggga gacctgatct attccgattt tgacgggatc gtggtggttc
      121 cccaggggat agaagacgaa gtgtttcagc gggcaaaaga gaaggtggag aatgaaaacc
      181 attcccgccg ggaactgctg gacggccgct cgctgcggca ggtctatgat aaatacaaat
      241 cactataacc ggccagcggg aagccggctt gacggactct ccggagaata ataaatgtgg
      301 ctctaacctt ttatacagga tcgcataatt aaagcgcaaa atcgggtaag tgaacccctt
      361 ctttgtcctg tagatttgaa aattaataac agaatggtaa tctttttaaa gacatcggca
      421 acccgtgtct gatcgttctg aaatttgggt tttaaattat atccaaacta atcactaaag
      481 catctgctat gaacttacat tcacggacac tgcgcaattg gcaaaagagg gtccttctgc
      541 aagccctgtt tgtctttttt gccttattct cctttgcaca aaaaataacc ctttcaggac
      601 cagtcacgga tggaaaggcg ccgctggaaa aagtatccat ccgggtactg ggatccggcc
      661 ggggcactac caccaatgag aacggtgaat tcaaaatttc cgccgttaaa ggccagaccc
      721 tggtattcag ctatctgggt tttgaagaaa aaagagtgct ggtcggcgat caaacggaga
      781 tcgcagtaag tttaacggca tccgcgcagg atgcgctgaa cgatgtggtg gttgtcggtt
      841 acggcaccaa aagaaaactg aatgttaccg gagccctctc aacggtatct gcgaaacagc
      901 tggagaacag gccggtaacc aatgtggcgg atgccctgca gggaacgatg gccggcgtaa
      961 ccgttgtcca gaacagcggg caaccgggca aggatcaggg tacgatacgg gttcgcggaa
     1021 tcggaacgct gggcaatagc gacgccatgg tagtggtgga cggaattgtt tccacgctga
     1081 atgacatcaa ccccaacgac atcgagtcca taaccgttct gaaagatgcc gcatctgctt
     1141 ccatttacgg gtccagggca gccaacgggg tgatcctggt caccaccaaa aaaggtaaga
     1201 acggcgccac cgtcgtgcat tataatgcgt acgtgggtaa acagcaaccc acccgcctgc
     1261 cggattatct tccttcctgg caggcttctt ctttttataa tgaagcgctg gtgaacgaag
     1321 gaaaaacacc gcggtattcg gatgccgaaa tacagaaatt caaagacgga tccgatccgg
     1381 acaactatcc caataccgac tggctcggat tattctacaa aggatcgggt tttcagcaga
     1441 gccattatgt agacgtcagc ggcggaaatg aaaaaacaca aagcctgctt tccttaggct
     1501 atttctcgga agacggtatc gttaaaaata ccggactcga ccggtacacc acccggttca
     1561 agatcagcac caggctgggc tcccggttta gtgtaaatgg aaatttcgcg tataccctgg
     1621 agaattttaa agagcccgtc gatggccaga acaggggctt tcaggccctg atccatggaa
     1681 tcaaccggat cgggaatgtg gttccctaca aatattcaaa tggatattac gggtttaacg
     1741 atgagggaaa tccggtggcc tcggtagacg agggcggtct ggcgcagacc aacacccatt
     1801 atcttaaagg cgtagtggat ggagatctgg aaatcatcaa gggactgcat tttaaacccc
     1861 tgctgggcta tcagcttacc atcgatcaat ccaagaactt tataaaggat atgcagtttt
     1921 atgactggca gaccggggcg cctgctctat acctgggacc caattccctg acgaatacga
     1981 ataatatcac cagcgtggtg acgctccagg gattgctgca atacgataaa aactggggcg
     2041 accacgactt cagtgtgctg gccggttatt cccaggaata tacgcacttc agtaacctga
     2101 tgggataccg gaaagatttt ctcaacaact ccctaagcga actgaatgca ggacccgtgg
     2161 ccggtcagca ggcgacgggc tcagcctacg aaattgcatt gcagtctttt ttcggccgga
     2221 taaattattc ctataaaggc aaatatttgc tggaaggcaa tatccgcgag gacggttcat
     2281 cccgttttgc ttcggataac cgctggggcg tttacccctc cttctctgcc ggatggcgga
     2341 tttcagaaga aggatttttc agcccgctga aaacgctttt aaacgatttg aaattccggg
     2401 gatcctgggg taaactgggc aaccagaatc tgagcaccat ccaaaacagc agtggcagcc
     2461 cgatcgggaa ctatccgtac atatccacca tttcttccgg tcagaactat accttcggag
     2521 atgccgtggc atccggtgtg gcaccgctga acggcgccaa tgcaatgatc cagtgggaga
     2581 gcaccacctc taccgatttt ggttttgatg ccagtttcct gcagggaaaa ataaccctca
     2641 ctgcagatta ttttatccgc aatacggaca acctgttatt gaatattccg gtaggagctg
     2701 tttatggctt taacgggccg gtgcaaaacg gcggcagcat tcaaaacaaa ggcctggaac
     2761 tggaactggg atatcacgga cggcaaaagg attttaccta taacg
//
LOCUS       DASYCU010000329         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5147136, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000329 DASYCU010000000
VERSION     DASYCU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5147136"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VGM24_06655"
     CDS             <1..181
                     /locus_tag="VGM24_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155086.1"
                     /translation="KEWKFKDADGSNTEMTIPVKELLDARKNSVSGVSLYYVAEGRAE
                     GQKLVAVGPVKKTNT"
     gene            531..1394
                     /locus_tag="VGM24_06660"
     CDS             531..1394
                     /locus_tag="VGM24_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY4155087.1"
                     /translation="MLKSQMAICLCSSLSLLPTSATPDKSMPAGMKHSVENLRKTSPV
                     VSKMTLNKRNLQFVRNYIQKSKENLVLIKRRSAVPFSIIDSVFKHYRLPHELKYLAVI
                     ESELNQSALSRVGALGPWQFMPGTAHILGLKARSGYDERRNYYKSTRAAARYLKDLYA
                     EFGDWLLVIAAYNGGPGAVYGAISKSGSRNFWVLQRYLPQETRLHVKKFIATQYFFEG
                     AGSVAGTAKAEILPKIKTGYIAPVAAIAIPKPVRAADPPRAETAEEKFQRLMKESEIL
                     LQKSNELLKKG"
     gene            complement(1432..>1787)
                     /locus_tag="VGM24_06665"
     CDS             complement(1432..>1787)
                     /locus_tag="VGM24_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155088.1"
                     /translation="ALQGDGELAGNALETSLDIEFTVRIIRKENGTINNPRVEDSGYL
                     MSVGLGKSLDDALKAATAGLFDWLQKDYRLSYAEATQVLSTSIEYTIAEIADPEVEIV
                     ARIKKEILENLKKQD"
BASE COUNT          468 a          444 c          437 g          438 t
ORIGIN      
        1 gaaagaatgg aagtttaagg atgcggacgg atcaaatacg gagatgacca tcccggtaaa
       61 ggaactgctg gatgcgcgga aaaattcagt gtctggggta agtctttatt atgtggcaga
      121 aggccgtgcc gaggggcaaa aactggtggc ggtagggcct gtaaaaaaga ctaatacctg
      181 atttggaact gttcatttgc agattcaaaa gcccgctccc tgagcgggct ttgtttttct
      241 gtgcccggaa taccaggccc tttttgcctt ttcgatccgg tttatttcct gttctgaagt
      301 attgtcctga taaccagtct gctggacgat cgtagtttta cccgtgaaaa cccctatgaa
      361 ttgatggatt gccagagtat ctttaaacca tacagttccg aaaatgaaaa gcctgactca
      421 ccgtaccccc tggaacactg tataaacttt tccaatattc ctatacctga ttttcaaaag
      481 caataaatcc aagcctgggt tcggcattgt tttacgcata aattaaaacg atgttgaaat
      541 ctcaaatggc gatctgtctt tgcagctcac tttctctttt accgacctcc gcgactccgg
      601 ataaatccat gccggcgggg atgaagcaca gtgtcgaaaa cctccggaaa acgagcccgg
      661 tggtttcgaa aatgaccctg aataaaagga acctgcaatt cgtgcgcaac tacattcaaa
      721 aaagcaaaga aaacctggtg ctgatcaaac gccggagtgc ggttcctttt tccatcatcg
      781 attctgtttt caaacattac cggttaccgc atgaactaaa ataccttgcg gtgatcgaat
      841 cggaattgaa ccagtccgcg ctttcccggg ttggtgcatt aggcccctgg caattcatgc
      901 ccgggaccgc acacatatta ggattgaaag cccggtccgg atacgacgaa cgcaggaact
      961 attataagag cacgcgggcg gcagccaggt accttaagga tctctacgcg gaatttggtg
     1021 actggctgtt ggtgatcgct gcctataacg gcggcccggg agccgtctac ggagccatca
     1081 gcaaatccgg gagcaggaat ttctgggtcc tccagcgcta tctgccccag gaaacccggc
     1141 tgcatgtgaa aaaattcatc gcaacgcagt atttttttga aggcgcgggc agtgtggccg
     1201 gcacggcaaa agcggagatc ctgcccaaaa taaaaacggg ttatattgct ccggtcgctg
     1261 ccatcgcaat acctaagccg gtgagagcag cagatcctcc gcgagcagaa actgccgaag
     1321 aaaaatttca acggctgatg aaagaatcag aaatattatt gcaaaagtca aatgaactgc
     1381 tgaaaaaggg atgatctcag aaggctggcc tcattcaggt aaagggccgg ctcaatcctg
     1441 ttttttcagg ttctccagga tttctttttt gatcctggca acgatctcca cctccggatc
     1501 ggcgatctct gcgatggtat attcgatgga agtactcaac acctgggttg cttccgcata
     1561 ggaaagacgg taatcctttt gcagccagtc gaacaggccg gcggtcgccg ccttcagcgc
     1621 atcatccagg ctcttgccca atccaactga catcaggtat ccggaatcct ccacccgggg
     1681 attgttgatg gtcccgtttt ctttcctgat aattcttacc gtaaactcaa tatccaggga
     1741 agtttccagg gcattgcccg ccagttcgcc atcgccctga agggcat
//
LOCUS       DASYCU010000330         6119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_652454, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000330 DASYCU010000000
VERSION     DASYCU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652454"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VGM24_06670"
     CDS             <1..925
                     /locus_tag="VGM24_06670"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY4155089.1"
                     /translation="MTRKAFENAITVVMVLGGSTNAVLHLIAMAKSVDVPLSLNDFQA
                     ISNRIPVLADFKPSGKYLMEDLHQFGGVPAVMKYLWKKGLLHGDCLTVTGKTLAENLA
                     TVPDLDFDKQKIIMPLEKPLKATGHLQILYGNLAEGGSVAKISGKEGERFEGTARVFD
                     GEHDLIKGIQTGKVHAGDVVVIRNIGPKGAPGMPEMLKPTGAIIGAGLGKSVALITDA
                     RFSGGTHGFVVGHITPEAFEGGLIALVEDDDIIEIDARKNTLTLKVADELIAERRKKW
                     KKPALKASRGVLFKYARSVKNASEGCVTDED"
     gene            974..2716
                     /gene="ilvB"
                     /locus_tag="VGM24_06675"
     CDS             974..2716
                     /gene="ilvB"
                     /locus_tag="VGM24_06675"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY4155090.1"
                     /translation="MEALTIKNDKKTQEKKKARKLSGSAALLEIFLAEKVDTIFGYPG
                     GAIMPIYDALYDYQDKLKHILVRHEQGAAHAAQGYARVSGKPGIVFATSGPGATNLVT
                     GLADAMIDSTPLVCITGQVYAHLLGTDAFQETDVINITTPVTKWNYQVTDATEIPSVL
                     AKAFYLASSGRPGPVLIDITKNAQLQAFDFEEYVPCEHIRSYRPKPIIRKKYIAEAAK
                     LINEAKRPFILFGQGVLLGGAEKEFIQFLEKSGIPAAWTILGMSAIPTNHPLAVGMLG
                     MHGNYGPNVLTNECDVLIAVGMRFDDRVTGRLDKYAKQAKVIHLDIDPAEIDKNVKTT
                     VPVWGDCKETLPLLTRLIDKKNYPQWLQKFRELQKEEDDKCIFPELHPATGILSMGEV
                     IDALNELTGGNAIIVTDVGQHQMVACRYAKFNQSKSNITSGGLGTMGFALPAAIGAKF
                     GAPERAVIAIVGDGGFQMTLQELGTIMQTGLNVKIIILNNHFLGMVRQWQQLFNDKRY
                     SFVNIESPDFVQLAKAYNIQAQRLTDRAEIRNALRAMLDHDSSYLLEVMVGKENNVFP
                     MVPQGCSVSEIRLM"
     gene            2776..3387
                     /gene="ilvN"
                     /locus_tag="VGM24_06680"
     CDS             2776..3387
                     /gene="ilvN"
                     /locus_tag="VGM24_06680"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY4155091.1"
                     /translation="MNGKKEIIMKQEYTITVYTENQIGLLNRIAIIFSRRKINIESMN
                     TSPSEIEGIHRFSIVINETEEVVKKLCRQIEKQVEVLKAYYNQNDEIVWQELALYKVS
                     TDLIAEKVKVERLLRKYGARAVVIRKDYTVFETTGHREETDGLLAVLEPYGLIEFVRS
                     ARVAIINASSGFHEKLKEFEQTEPGKELIENEFLDKNNEVFSM"
     gene            3427..4518
                     /gene="ilvC"
                     /locus_tag="VGM24_06685"
     CDS             3427..4518
                     /gene="ilvC"
                     /locus_tag="VGM24_06685"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY4155092.1"
                     /translation="MATLNFGGVDETVVTREEFPLSKAQQVLKDEVVAVIGYGVQGPG
                     QALNQKDNGINVIVGQRKNSKTWDKAVRDGFVPGKTLFEIEEALEKGTIICYLLSDAA
                     QIELWPTVKKHLTRGKALYFSHGFGITFKEQTGIIPPADVDVFLVAPKGSGTSLRRMF
                     LQGRGLNSSYAIFQDATGKAKDRVIALGIAVGSGYLFETNFKKEVYSDLTGERGTLMG
                     AIQGIFAAQYQVLRDKGHTPSEAFNETVEELTQSLMPLVAENGMDWMYANCSTTAQRG
                     ALDWWKKFRDATAPVFKELYESVASGKESQRSIDSNSKTDYREKLEVELKELRDSEMW
                     QAGKTVRSLRPENQPSPAPKTDKKLAEKV"
     gene            4543..5799
                     /gene="ilvA"
                     /locus_tag="VGM24_06690"
     CDS             4543..5799
                     /gene="ilvA"
                     /locus_tag="VGM24_06690"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY4155093.1"
                     /translation="MKTSTISLPKLDVAKAAERVKKVALRTPLMLNQNLSRRYQCNVY
                     LKREDLQVVRSYKIRGAYNMMSSLPEFKIRNGVVCASAGNHAQGFAYSCKKLNGKGVV
                     FMPVITPKQKVDQTKMFGEDFVEIRLTGDTYDDCAVAAQLFTPEHNMTFIPPFEDLRI
                     IEGQATVALEILEDLSDIDYLLVPVGGGGLASGTGLYFKNHSPGTKLIGLEPEGAPSM
                     FEAFKAGHPVTLDKIDRFVDGASLKRTGDLTFSICREVLDELILVPEGRICTSILKLY
                     NEDAIVVEPAGALSIAGLESMAESIKGKNVVCILSGSNNDINRMPEIKERSLQYEGLK
                     HYFLIQFAQRPGALKEFVNNVLGPSDDITRFEYMQKTNKESGPALVGIELQNRSDYHR
                     LMENLKVRHMDFSEINKNDTLYGYLS"
BASE COUNT         1735 a         1455 c         1636 g         1293 t
ORIGIN      
        1 catgacccgt aaagcatttg aaaatgcgat tacggtagtg atggtcctcg ggggttccac
       61 caatgccgtg ctgcatttga ttgcgatggc aaaaagtgtg gatgttccct taagcctgaa
      121 tgattttcag gccatcagca accggattcc ggtgctggcc gattttaagc ccagtggaaa
      181 atacctgatg gaagacctgc atcaatttgg cggggtgccg gcggtaatga aatacctgtg
      241 gaaaaaaggt ctcctgcatg gcgactgttt aaccgtaacg ggcaagaccc tcgccgaaaa
      301 cctggccacc gtccccgatc tcgattttga caaacaaaaa ataattatgc ctttggaaaa
      361 gccattaaag gcgaccgggc atttacagat attatatggc aacctggccg aaggcggaag
      421 cgtggcaaaa atcagcggca aggaaggcga acgattcgaa gggacggcca gggtttttga
      481 tggcgaacac gacctgatca aagggataca aacaggaaaa gtgcatgcgg gcgatgtggt
      541 cgtcatccgg aatatcggac cgaaaggggc accgggcatg cccgaaatgc tcaaacccac
      601 cggcgcgatc atcggcgcag gcctcggcaa aagcgttgcg ctgatcacgg atgcccgctt
      661 cagcggagga acgcatgggt ttgtggtggg ccatattacc cccgaagcgt ttgaaggcgg
      721 actgattgcc ctggtggaag atgatgatat catcgaaatc gatgcccgaa aaaataccct
      781 cacgttaaaa gtggcggatg agctgatcgc cgaaagacga aaaaaatgga aaaagccggc
      841 cctgaaagcc agccgcggag tactttttaa atatgcccgg agtgtaaaga atgcatccga
      901 aggctgtgtg acggatgagg attgagcttt gctgtgaaaa tggaatattt taatacacga
      961 taaattatgc aggatggaag cgcttacaat taaaaatgac aagaagaccc aggaaaaaaa
     1021 gaaggcccgg aaactgtcgg gatctgctgc cctgctggaa atatttctgg cagaaaaagt
     1081 ggataccatt tttggatatc ccgggggagc tatcatgccc atttacgatg ctttatacga
     1141 ctaccaggac aaactgaagc atatcctggt gcgtcatgag cagggagctg cgcatgccgc
     1201 acagggttat gcacgggttt cgggaaaacc gggcattgtg tttgccacca gcggccccgg
     1261 cgccaccaac ctggtgacgg gcctggccga cgccatgatc gacagcaccc cgctggtctg
     1321 cattaccggt caggtatatg cgcacctgct cggcaccgat gcttttcagg aaaccgatgt
     1381 gatcaacatc accacgccgg tgaccaagtg gaattaccag gtcaccgatg caacggaaat
     1441 cccttcggtg ctggccaagg cattttatct ggcgtccagc ggacgccccg gcccggtatt
     1501 gatcgatatt accaaaaacg cccagctgca ggcattcgat tttgaggaat atgttccgtg
     1561 cgaacatatc cggagttacc ggccgaaacc catcatcagg aaaaaatata tagccgaggc
     1621 ggcaaagctc atcaatgagg ctaaaaggcc ttttatcctt ttcggtcagg gcgtcttgct
     1681 cggcggcgcc gaaaaagaat tcatccagtt cctggagaaa tccggaattc cggcggcgtg
     1741 gaccatcctc ggcatgagcg ccatcccgac aaatcatccc ctggcagtgg gtatgctggg
     1801 catgcatggc aactacggac ccaacgtgct caccaatgaa tgcgatgtgc tgatcgccgt
     1861 gggcatgcgc ttcgacgacc gcgttaccgg caggctggat aaatatgcaa aacaggctaa
     1921 ggtgatccac ctggatattg atccggcgga aatagataag aacgttaaga caaccgttcc
     1981 ggtttggggc gattgcaaag aaacccttcc gctcctcacc cggctaatcg acaaaaaaaa
     2041 ttatccgcaa tggctgcaga agttcaggga gctccaaaaa gaagaggacg ataagtgtat
     2101 ttttccggag ctccatccgg cgaccggaat attgtccatg ggcgaagtga tcgacgcgct
     2161 gaacgaactt accgggggaa atgcgatcat cgtgaccgac gtgggccagc accagatggt
     2221 agcctgccgt tatgcaaaat tcaaccagtc gaagagtaac attacttccg gcggactggg
     2281 taccatgggt tttgccttac ccgcagccat cggggccaaa ttcggcgcac cggaaagagc
     2341 cgtcattgcc atcgtgggcg acggcggatt tcagatgacc cttcaggaac tcggtaccat
     2401 tatgcaaacc ggcctgaatg tgaaaatcat aatcctgaac aatcacttcc tgggtatggt
     2461 gcggcagtgg caacaattgt ttaacgacaa aagatattcc ttcgtaaaca tcgaaagccc
     2521 ggattttgtc cagctggcca aagcatataa tatacaggcg caacgcctga cagaccgggc
     2581 agaaatccgg aatgccctca gggcaatgct ggatcacgac tcgtcctatc tgctggaagt
     2641 gatggtggga aaggagaata atgtgtttcc catggtgccg cagggatgca gtgtttcgga
     2701 aatcaggttg atgtaataaa aaatattata cccggaaagt aatttgacag cagccatata
     2761 atttaaaacc cgtcagtgaa cggaaagaag gagatcatta tgaaacaaga atatacgata
     2821 accgtgtaca ccgaaaacca gatcggcctg ctcaaccgga tagcgatcat cttctcgcgg
     2881 agaaagatca atattgaaag catgaacacc tctccctccg aaatcgaggg catacaccgg
     2941 ttcagcattg tgatcaacga aacggaagag gtggtgaaaa agctttgccg ccagattgaa
     3001 aaacaggtgg aagtgttaaa ggcttattac aatcagaacg acgagatcgt ctggcaggaa
     3061 cttgccctgt acaaagtatc caccgatctc atagcggaaa aggtgaaagt ggaaaggctg
     3121 ttaagaaaat acggcgcccg ggccgtcgtg atccgtaagg attatacggt ttttgaaacc
     3181 accggccacc gcgaggaaac cgacggactg ctggcggtgc tcgaaccgta tggcctgatt
     3241 gaattcgtgc gcagcgcgcg cgtggccatt attaatgcca gtagcggttt tcacgaaaaa
     3301 ttaaaggaat tcgaacaaac ggaaccgggt aaagaactca ttgaaaacga gtttttggat
     3361 aagaacaatg aggttttcag catgtaattc attcaattat tttattaaat caataattca
     3421 aacagtatgg caacacttaa tttcggcgga gtggatgaaa cggtggtaac ccgtgaagaa
     3481 ttcccgctct cgaaagccca gcaggtatta aaggatgaag tggtcgccgt gatcggctac
     3541 ggcgtacagg gccccggtca ggcattgaac cagaaagata acggcatcaa tgtgatcgta
     3601 ggccagcgca agaattcaaa aacctgggat aaagccgtac gcgacggatt cgttcccggc
     3661 aaaacgcttt ttgaaattga agaagcattg gagaaaggaa ccattatctg ttacctgctg
     3721 agtgatgcgg ctcaaattga actgtggcca accgtaaaaa aacacctgac ccggggcaag
     3781 gctttatatt tctcccacgg attcgggatc acgttcaagg aacagaccgg aatcataccg
     3841 ccggccgatg tggatgtttt cctggtagcc ccgaaaggtt caggaacttc attgagaagg
     3901 atgttcctgc agggacgcgg acttaacagc agctatgcca tcttccagga tgctaccgga
     3961 aaagccaaag accgcgtaat tgcgctcggc atcgccgtag gcagcggata tttatttgaa
     4021 acgaatttta aaaaggaagt ttacagcgac ctcaccggcg aacgcggcac cctgatgggc
     4081 gccatccagg ggatttttgc tgcgcagtac caggtgcttc gcgataaagg gcatacgcct
     4141 tccgaagcct tcaatgaaac cgtggaagaa ctcacgcaga gcctgatgcc gctggttgcc
     4201 gaaaacggaa tggactggat gtatgccaac tgctccacga ccgctcaacg gggtgcgctc
     4261 gactggtgga aaaaattccg ggatgctacg gctccggtgt ttaaagagct ttatgaaagc
     4321 gttgccagcg gaaaagaatc gcagcgttcc atcgatagca acagtaaaac agactaccgg
     4381 gaaaaactgg aagttgaact gaaagaattg cgggatagtg aaatgtggca ggcgggcaaa
     4441 acagtcagaa gcctgcggcc ggaaaatcag ccttccccgg ctcccaaaac agataagaaa
     4501 ctggcagaga aagtgtgagg ccggcagcgt aaaatggtta gcatgaaaac atcgacgata
     4561 tcccttccga agctggatgt ggcaaaggcg gcggaacgcg ttaaaaaagt ggcgttgcgg
     4621 acgcccctta tgctgaatca gaatctttcc cgaaggtatc agtgcaatgt ttatctgaag
     4681 cgggaagatt tgcaggtggt tcgctcatat aaaatacgcg gcgcctacaa catgatgagc
     4741 agtctaccgg aatttaagat ccggaatggc gtcgtatgcg caagtgccgg aaaccatgcc
     4801 cagggatttg cttattcctg taagaaactc aacgggaaag gcgtggtttt tatgcccgtt
     4861 atcacgccca aacaaaaagt ggatcagacc aaaatgttcg gggaggactt tgttgaaatc
     4921 agactgaccg gagacacgta tgatgattgc gccgttgccg cccagctatt cacgccagaa
     4981 cataatatga ccttcatccc gcccttcgaa gatctccgga tcatagaagg acaggcgacg
     5041 gtggccctgg aaatcctgga ggatctgtct gatattgatt acctgctggt tcccgtcgga
     5101 ggaggcggcc tggcttccgg aacaggcctg tatttcaaaa accattctcc cggaacaaaa
     5161 ctaatcgggc tggaaccgga aggagccccg tccatgttcg aggccttcaa ggccggccat
     5221 ccggtcaccc ttgataagat cgaccgcttt gtggacggcg cttccttgaa aaggaccggg
     5281 gaccttacct tttccatctg ccgggaagtg ctcgacgagc tgatcctcgt gccggaaggg
     5341 cggatctgca cgagcatcct gaaactgtat aatgaagatg ccattgtggt ggaaccggcc
     5401 ggggccctgt ccattgccgg gctggaatcc atggcggaat ccattaaagg gaagaacgtg
     5461 gtctgcatcc tgagcggcag caacaacgat ataaaccgga tgccggagat caaagaaaga
     5521 tccctccagt atgaaggcct caaacactat ttcctgatcc agtttgcgca acggcccgga
     5581 gcacttaagg aattcgtaaa caatgtgtta ggaccgagcg atgacatcac ccggtttgaa
     5641 tacatgcaaa agaccaataa ggagtccggg cccgccctgg tcgggataga gctccaaaac
     5701 cggtcagact atcaccggct catggaaaac ctgaaggtcc ggcacatgga tttttcggaa
     5761 attaataaaa atgatacgct atacgggtac ctgagttaaa cataaaccct gcggaaagtg
     5821 ttgatatata tttaataaaa tatcggcact tttttatatt tttagacgaa tcggctgctt
     5881 ccttagaaaa atcatacata taacgattgt ccgttgatgc cattcactgc ttaatcgaaa
     5941 ataatggaaa gcaatcaagg attataccat ccttcctttg agcgggatgc ctgtggcatc
     6001 gggtttgtcg ccaatattaa aggcaataag tcccaccaga tcatttcaga tgccctgacg
     6061 attctggaaa acatggaaca ccgcggggcc tgcggttgcg aaaccaatac cggggacgg
//
LOCUS       DASYCU010000331         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1231170, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000331 DASYCU010000000
VERSION     DASYCU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231170"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGM24_06695"
     CDS             complement(<1..266)
                     /locus_tag="VGM24_06695"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase"
                     /protein_id="HEY4155094.1"
                     /translation="MLRNDLYTIDTAAENNSVLLVSVRLNANHSVFKGHFPDHPVLPG
                     VCMLEMVREILEISLHEKLRISKGPLIKFLSMIVPDKSPVVSIEV"
     gene            complement(272..895)
                     /locus_tag="VGM24_06700"
     CDS             complement(272..895)
                     /locus_tag="VGM24_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155095.1"
                     /translation="MQISPIRCLLFLSLICGMLSCNAYRHLEKTNADFGCLQQFKPDI
                     KRALYRTSVDITGKHISGLLLVKTLPDSSLRIAFSNAMGFSFFDIGFLPGNEFKVYQI
                     LPQMNKAAVIKTLRKDFELVLFRKMDGKNRFALKDSSLIYYGFPQEKGVNYYITDTNC
                     SRLVKMQRASKRKPVVEAVMFLDSGSVPDSVSIRHLNFNFSISLKKI"
     gene            complement(858..1493)
                     /locus_tag="VGM24_06705"
     CDS             complement(858..1493)
                     /locus_tag="VGM24_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY4155096.1"
                     /translation="MRKCCLGLICILLAVSVKAQYAGFTLVRDQDNFKLAFNEATRKT
                     NSIKSDFIQEKNLSMLSDKITSRGKFWFKKENRVRMEYTRPFDYLLILNGGRIYVKDG
                     QKENKVQAGSNKVFQQVNRILLDCVGGTVLSNPDFQTRVFENAKSYLIELAPVAKNLK
                     DLYQKINIFIDKKDYTASAIDMFEVSGDNSSIRFLNKELNANIPDTLFTIP"
     gene            complement(1486..2259)
                     /locus_tag="VGM24_06710"
     CDS             complement(1486..2259)
                     /locus_tag="VGM24_06710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY4155097.1"
                     /translation="MLNYRNSNIFFILFIIAWVWLDSQRHFSPMAYGWILLAYLSVLF
                     CGSYFIQLGFFFKSICSGPHTQREIALSFDDGPDPFFTPVILDLLKERGVETIFFCIG
                     KKIPGNENILHRIVAEGHLIGNHSYSHQRFFDLFGPDKMHEDLQQMNEAIQAATGLLP
                     RLFRPPYGVTNPNLKKAVERNRFISIGWNLRSYDTVIRDENRLMQRVLRRIKPGSILL
                     FHDTSETTVKILPDLIGKVQQDGYRIVRLDKMLKLKAYA"
     gene            complement(2253..3299)
                     /locus_tag="VGM24_06715"
     CDS             complement(2253..3299)
                     /locus_tag="VGM24_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY4155098.1"
                     /translation="MKVYIRSAGNISPQHSLEPELFFSKREIYSGSRMKAIEPDYSLF
                     VDSKMIRRMSRIVKMGVASALVGLRNAATDMPEAIVTGTAYGCLEDTGVFLRRMIDNG
                     EELLTPTAFIQSTHNTVGAQIALLLKCHGYNNTIVHRGFSFENALIDASLLLQEGMNN
                     VLVGGIDEITDDSHAILSRFHIYGKGNNADFQNKKFKGTIGGEGSSFFLLNNKAVAGT
                     LAELSSLKTIYKPESAAAVEEEIQQFLEESSLKPEDLDLILTGSNGDETHDAIDQYLS
                     ARIFKNIPAYPFKLVCGEYPTASAFAVWLAATLAGGQKIPDWFTPLRLPLRKILIHNH
                     DMNNFHSLFLISAC"
     gene            complement(3308..>4449)
                     /locus_tag="VGM24_06720"
     CDS             complement(3308..>4449)
                     /locus_tag="VGM24_06720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY4155099.1"
                     /translation="AASLQAFRSGEAGMKEIRGLRTRQKGKLPVAEVPFSNRELAEQS
                     GLSPETPRTALLSAIAAREAIKDSGIENVKAIRTGFISANTVGGMDKTEDFFESFMKD
                     PASGKLRDVVRHECGAITEIVADHLGITHFSSTISTACSSSANAVFTGARMIRHGLLD
                     MAVAGGADALTRFTLNGFNTLMILDKEYCKPFDENRKGLNLGEGAGYLVLLSEKMMRR
                     ANKDPYCILSGYSNANDAYHQTASSPEGTGSFLAMKNALAGSGLRPGDIDYINLHGTG
                     TQNNDIAEGTAIKLLFDPVYPKMSSTKSFTGHTLGASGGMEAVFSALAIRHGIIYPNL
                     RFETAMKELPFTPETKFLTRQRVRHVLSNSFGFGGNCSSLIFSAA"
BASE COUNT         1057 a         1118 c         1149 g         1125 t
ORIGIN      
        1 acttcaatgg agaccacggg gcttttgtcc ggaacgatca tcgagaggaa tttgatcagc
       61 gggcctttgg agatccggag cttttcatgc agcgagattt ccaggatctc ccggaccatt
      121 tccagcatgc agaccccggg tagcaccgga tgatcgggga aatggccttt gaatacggaa
      181 tgattcgcgt ttaaccggac ggaaaccagc aggaccgaat tgttttcagc agccgtatca
      241 atggtataaa ggtcattcct gagcaaggcg gttatatttt tttcagactg atggaaaaat
      301 taaaattgag gtgccggata gacacgctgt cgggcacgga tccggaatct aagaacatga
      361 ctgcttccac cacgggtttt cgtttggaag ccctttgcat tttcaccagc ctggaacaat
      421 tcgtatcggt aatataataa ttaactcctt tttcctgtgg aaatccataa tagatcaggg
      481 aactgtcctt cagggcaaac cggttctttc catccatttt ccggaacaaa accaattcaa
      541 aatccttcct cagcgtttta atcaccgccg ctttattcat ctgcggaagg atctggtata
      601 ccttaaactc attgccggga agaaagccga tatcgaaaaa gctaaagccc atggcgtttg
      661 aaaaagcgat ccggaggctg ctgtccggca gggtcttgac cagcagcagg ccgctgatat
      721 gcttgccggt aatgtccacc gaggtccggt agagcgccct ttttatatcc ggtttgaatt
      781 gctgcaggca tccaaagtcc gcattggttt tctccaggtg ccggtaagca ttgcaggaaa
      841 gcatcccgca gatcaggcta aggaatagta aacagcgtat cggggatatt tgcattcagt
      901 tctttattga gaaatcggat gctgctgtta tctccggaca cttcaaacat gtcaatagca
      961 gaagccgtgt aatctttctt atctataaaa atattgatct tttgatacaa gtccttcagg
     1021 tttttagcca ccggcgccag ttcgatcagg tagctcttcg cattttcaaa gacccgggtt
     1081 tgaaaatcgg ggttggacaa taccgttccc cccacgcaat ccagcaggat ccggtttacc
     1141 tgctgaaata ccttattcga accggcctgg accttattct ctttctgacc gtccttcaca
     1201 tagatccggc cgccattcag gatcaggaga tagtcaaagg gccgggtata ttccatacga
     1261 acccggtttt cctttttaaa ccagaacttt ccccgggacg tgatcttgtc ggaaagcatg
     1321 ctgagatttt tctcctggat aaaatcactt ttaatactgt ttgttttccg ggtagcttcg
     1381 ttgaaagcca gtttaaaatt atcctgatcc cggacaagtg taaatccggc atattgcgct
     1441 ttaacggaaa cggcgagcag tatacagatc aggccaaggc aacacttacg cataggcctt
     1501 caatttaagc attttatcca gtcgcacgat ccggtatccg tcctgctgaa cctttccgat
     1561 caggtccggc agaattttaa cggttgtttc actcgtatcg tggaaaagca ggatggatcc
     1621 gggttttatc cgccggagca ctctttgcat cagccggttc tcatcccgga tcacggtgtc
     1681 gtaggaacgc agattccagc cgatggagat gaacctgttc cgttcaactg cttttttcag
     1741 attcggattg gtgactccgt agggcggccg gaacagccgg ggcaggagcc cggtagcggc
     1801 ctggatcgct tcgttcatct gttgcaggtc ctcgtgcatt ttatccgggc cgaacagatc
     1861 aaaaaaacgc tggtgcgaat agctgtggtt gccgatcagg tggccctcgg caacgatccg
     1921 gtgcaggata ttttcattgc ccgggatctt tttcccgatg cagaaaaaaa tcgtctccac
     1981 tcctctttct ttcagcaggt cgagaatgac cggggtaaaa aacggatcgg gcccgtcgtc
     2041 gaaactaagc gcgatctctc tttgcgtgtg cggacccgag cagatggatt taaaaaaaaa
     2101 gcccagctga ataaaataac ttccgcagaa aagtacggag aggtaggcca gcaggatcca
     2161 tccataggcc atgggagaaa aatgccgttg actgtccagc catacccagg cgatgatgaa
     2221 aaggataaag aaaatattgc tgttccggta gtttaacatg cagagatcag aaataagctg
     2281 tgaaaattgt tcatatcgtg attgtggatc aggatcttcc ttagcggtag tcggagggga
     2341 gtgaaccagt ccggtatttt ctgcccaccg gccagcgttg cggccagcca taccgcgaac
     2401 gcggatgccg tgggatattc tccgcatacg agcttgaacg gataggcggg aatgttctta
     2461 aaaatgcggg ccgataaata ttgatcgatc gcgtcgtggg tttcatctcc gttactaccg
     2521 gtgaggatca ggtccagatc ctccggcttg agggaagatt cttccaggaa ctgctgaatc
     2581 tcttcctcca ccgccgccgc gctttccggt ttatatattg ttttcaggga acttaattca
     2641 gccagggttc cggcaaccgc tttattgttc aggagaaaga aagaggagcc ttcgccgcca
     2701 atggttccct tgaatttttt gttctggaaa tccgcgttgt ttccttttcc ataaatatgg
     2761 aaacggctca gaatggcatg actgtcatcc gtgatctcat cgatgccgcc aaccaggaca
     2821 ttattcatcc cttcctgcag gaggagcgag gcatctatca acgcattctc gaaagaaaac
     2881 ccgcggtgta caatggtatt attatatccg tggcatttaa gcaggagggc gatctgcgca
     2941 cccaccgtat tgtgcgtgga ttgaataaaa gcagtggggg tgaggagctc ttccccgtta
     3001 tcgatcattc tgcgtaagaa cacgccggta tcttcaagac atccgtaagc cgttcccgtt
     3061 acgatcgctt ccggcatatc ggtagcggca ttgcggagac ctaccagggc tgaggccacg
     3121 cccattttta cgatccggct catgcggcgg atcatcttgc tgtccacaaa aagcgaataa
     3181 tcgggttcaa tagctttcat ccggcttccg gaatatattt cccgtttgga aaaaaataat
     3241 tccggttcga gcgaatgttg cggggaaata tttccggctg aacgaatgta aaccttcata
     3301 caatggatta agcggccgaa aaaataaggg aggaacaatt ccccccgaac ccgaatgaat
     3361 tggaaaggac atgccggacc cgctgccggg tcaggaattt ggtttccgga gtgaatggaa
     3421 gctctttcat cgctgtttca aaacgcaggt tcggatagat gatcccatgg cggatggcga
     3481 gcgccgaaaa aacggcttcc atgccgccgc tggcgcccag ggtatgcccg gtgaacgatt
     3541 tggtcgaact cattttcgga taaaccggat caaacagcag tttaatggcc gttccttcgg
     3601 cgatatcgtt gttctgggtt ccggtcccgt gcaggttgat atagtcgata tctcctggcc
     3661 gcaatccact ccccgcgagg gcattcttca tagccaggaa agatcctgtg ccttccggag
     3721 aagacgcggt ctgatgatag gcgtcattgg cattgctgta cccggacagg atacaatagg
     3781 ggtctttgtt cgcccggcgc atcatcttct ccgagagcag caccaggtac ccggcgcctt
     3841 cgcccaggtt cagccctttc cggttttcat caaagggctt gcagtattcc ttgtccagga
     3901 tcatcagggt attgaaacca ttcagcgtaa aacgggtgag ggcgtcggcg ccgcctgcca
     3961 ccgccatatc cagcagaccg tgccggatca ttcgcgcgcc ggtgaacact gcgttcgcag
     4021 aagaggaaca ggccgtgctg atggtgctgc tgaaatgcgt tattcccagg tgatcggcca
     4081 cgatctcggt aatggcgccg cattcatgcc ggaccacatc gcggagtttt ccggaagcgg
     4141 ggtccttcat gaaggattcg aagaagtcct ccgttttatc catcccgccg acggtattgg
     4201 cggaaataaa cccggtgcgg attgctttta cattttctat tccggaatct tttatggctt
     4261 cgcgggcagc gatggcgctc aggagggccg tacgcggggt ttcgggagaa agaccgctct
     4321 gttcggccag ctcccggttg ctgaatggga cttccgccac cggtaatttt cccttttgcc
     4381 gcgtgcgcaa tccgcggatc tctttcatgc ccgcttctcc ggaccggaat gcctgcagcg
     4441 aagcggctg
//
LOCUS       DASYCU010000332         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1814069, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000332 DASYCU010000000
VERSION     DASYCU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814069"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..839
                     /locus_tag="VGM24_06725"
     CDS             186..839
                     /locus_tag="VGM24_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4155100.1"
                     /translation="MEKIRILIAEDHKLIRETWTFILNNDGRFAVVAACSDSAEAVDL
                     AEKERPDVILMDINMMPMNGFEATARFGEVTPESKVIGLSFYSEMLFVKKMFSAGAKG
                     YITKNSGREEMYEAILAVQAGQLFVCEEIRNKGIDLDTQPDADPAYIESLTNKEIQIS
                     QCIRQGLRSKDIADLLSISIKTVEVHRHNILRKMHVKNSISLVNLMNSKNGYVRYSF"
     gene            983..>1833
                     /locus_tag="VGM24_06730"
     CDS             983..>1833
                     /locus_tag="VGM24_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY4155101.1"
                     /translation="MYTIYLSVMYYWILYPPPFFLTRLLFIEWSVNSFLFFVAVFCAG
                     AAAFGIISRKYFPWEGNSIKPVFGEETSPDSVKNSGDFTTQKKIGDIVADLLRLIEDT
                     SDAVFSTDMDLKLITWSKASEKLFGYPEPEILGKEVLDVLAVRMPEGKRLAIRRQLAG
                     TGYWKGEMGFAPQNHSGLRVRVSMSLTRKDCGEPDGYLIVCRDITERKAHTDQPRQVN
                     EDSEKEGQENTKRTVQSEKKYKMLFDNNPMPMWMLSLDNFRIIEVNQAALRHYGYNRE
                     EFLAIDAR"
BASE COUNT          505 a          404 c          444 g          480 t
ORIGIN      
        1 ctgcagggtt tcgaataaca taatcagggg tttaatttat acgtaattaa cgattgactt
       61 ttttgtagtt cttattctac agggaaatgg atttttttcc cgtttaaata ggtgctttgt
      121 tctatttcag gcggcttcga tccatactat cttgcaccca ttgcagaagt tcaatcccca
      181 aaacaatgga aaaaattcga atcctgatcg cagaagatca caaactaatt cgtgaaacct
      241 ggactttcat cctgaataat gacgggcggt tcgccgtggt tgccgcctgc agtgattccg
      301 ccgaggcggt tgacctggcg gaaaaggaaa gaccggatgt aatcctgatg gacatcaaca
      361 tgatgcccat gaatggtttt gaagcaacag cccgttttgg ggaagttaca ccggagtcca
      421 aagtcattgg cttgtccttt tattcggaaa tgctgtttgt aaaaaaaatg ttctccgcgg
      481 gagcaaaggg atatataacc aagaattcgg gccgggaaga aatgtacgag gccattctgg
      541 cagtacaggc cggtcagctc ttcgtttgtg aagagatccg gaataaaggt attgacctgg
      601 atacccagcc tgatgccgat ccggcctata ttgaatccct taccaacaag gaaatccaga
      661 tttcccagtg catccgtcag gggcttcgtt cgaaagacat cgccgacctg ctcagcatct
      721 cgataaaaac agtagaagtt caccgccata atatcctgcg aaaaatgcac gtgaaaaatt
      781 ccatctcgct ggtaaatctc atgaattcaa agaacggcta cgtaagatat agtttctgat
      841 attatttatt gtttcccttt ttttgccgga atgcttattc cggcatttct gtttacggtg
      901 ctaaaggttt acccttacgc agattttccc ttccggggtc cttatcatgc ccgggtttgc
      961 tgaacggata aaatggatta aattgtacac aatctactta tccgtaatgt attactggat
     1021 cctttatcct cctccgttct ttctgaccag gctgctattc atcgaatgga gcgtgaactc
     1081 tttcctgttc tttgtcgcag tcttttgtgc cggcgcggcc gctttcggga tcatcagcag
     1141 aaaatatttt ccctgggaag ggaattccat aaaaccggtt ttcggggaag aaacctcacc
     1201 ggattccgtc aaaaactccg gggattttac cacacaaaaa aagatcgggg atatagttgc
     1261 ggatctcctc cggttaatag aggataccag cgatgccgtc ttctcgacag atatggatct
     1321 taaattgatt acctggagca aggcatctga aaaattgttt gggtaccccg aaccggaaat
     1381 attggggaaa gaggtcctgg atgttctggc ggtgcggatg ccggagggaa agcgcctggc
     1441 gatccgcagg cagctggccg gaacaggata ctggaaagga gaaatgggtt ttgcacctca
     1501 aaatcattcc ggactcaggg tccgggtctc catgagcctg acccggaaag attgcgggga
     1561 accggatgga tatcttattg tttgccggga tataacggaa agaaaagccc atacagatca
     1621 accccgtcag gtgaacgaag attcggaaaa agagggacag gaaaatacaa agagaactgt
     1681 ccagtctgaa aaaaaataca aaatgctttt cgacaacaat cctatgccga tgtggatgct
     1741 gtctctggat aatttccgga tcatagaggt gaaccaggcc gcgctccggc actacggata
     1801 taaccgggaa gaattcctgg caatagatgc gcg
//
LOCUS       DASYCU010000333         9270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1814331, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000333 DASYCU010000000
VERSION     DASYCU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814331"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGM24_06735"
     CDS             complement(<1..534)
                     /locus_tag="VGM24_06735"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4155102.1"
                     /translation="MQVLYKILFFLLLVFSLTISSCSEKAAATDTKEGYVLPDSLLKT
                     IEIDTVSTAKVLNSLTLTGKVDFDEDRILKIYPVVSGQVSRISVMLGDYVKKGQVLAV
                     LKSAEMAGFSNDLTLARSNLEVARKSLDAANDMYKSGLASQRDQLAARESYNQALSAL
                     QKAQRVLNLNGGSMDGDY"
     gene            complement(553..1833)
                     /locus_tag="VGM24_06740"
     CDS             complement(553..1833)
                     /locus_tag="VGM24_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4155103.1"
                     /translation="MLQKFISLLIFASFTGTGKAQIPGDSLRINLDSAEHLFISGNFS
                     LLALKYNIQAQQAQEIQAHLYPNPNLSASYSLYNTKTKKIFPVGPGESGGELAAQLSQ
                     VVYLAGQRNKQLKIARAGTRLAYYQFYDLVRTLKYTLRTDFFNIHYLLQSAAVYDEEI
                     KSLQQVVDAFNEQEGRGYIAEKEVIRVKAQLYALLNEYNDLNNQINDLQSELRLVLQV
                     KDSFIVPQVDTSVLRSFDPAAYPLNTLLDSAYKNRSDLLIARTNVDISKLNYNYQKAL
                     AVPNLTLSLAYDQQGSYINNLSSLGAAIDLPFFNRNKGNILTAKLNTEASTVLQQNME
                     ATVEENVFRSLQKAFSNDKMLKNIDPKFLADFDRMLNEVLLNYRKRNITLLDFLDFYN
                     SYKENILASNNILFNRLVAFEDINFYTGTEFFHP"
     gene            complement(1845..2540)
                     /locus_tag="VGM24_06745"
     CDS             complement(1845..2540)
                     /locus_tag="VGM24_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4155104.1"
                     /translation="MSNKAKVLLAEDDLSLGYVIKDNLVDAGYDVVLCPDGQTAIDKF
                     NKESFDICLLDVMMPNKDGFAVAKKIRQQTDIVPILFITSKSLEEDRIHGFESGGDDY
                     ILKPFSMQELMLRIEVFLRRTKKMYSERSQSFRIGEMLFSFNNLKLMVDKTEHNLTQK
                     EAELLQFLCEHPNRILKREEVLLNVWGKDDYFLGRSMDVFITKLRKYFKADPSANIET
                     IHGIGFRLNAVVQ"
     gene            complement(2571..3833)
                     /locus_tag="VGM24_06750"
     CDS             complement(2571..3833)
                     /locus_tag="VGM24_06750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4155105.1"
                     /translation="MRSTMLKWLVLSATIVIGLIVIVQVFWLTKVYSFEKKQFSTNIV
                     KSLRGVFEDLEMNDDPSLSLEQLIHNPQDDYYLFKADTIPEKDSLTHYLKKEFGDFDV
                     LTDVKLGAYSKLNKKYIYEEYVPTAASRYNVTGTSGLPVFTTDYDHILLYFPHRSLYI
                     LGEMNFWIVSSVALSLALVSLAISLFYFYRQKFLAEVQKDFVNNFTHEFKTPLAVIKI
                     AGDVLGQEDIVNKPDRLKRYASIIQNQTTHLQSQVEKLLKSASVENKKLPIDKQTINP
                     AFLIQQALNKLQPLIEQRQAKVELKVDNYETFIQGDESHLELAVVNLLENALKYSNSP
                     HIVVQAGREDSDYFISVKDNGIGIEKKYIKNIFKKFYRVPTGDVHDVKGFGLGLNFVK
                     RIVDGHDGKIRVNSLPGIGTEFRLLLPF"
     gene            complement(4306..7356)
                     /gene="secDF"
                     /locus_tag="VGM24_06755"
     CDS             complement(4306..7356)
                     /gene="secDF"
                     /locus_tag="VGM24_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HEY4155106.1"
                     /translation="MRALVSMFAGLLIVISLYQLSFTWFVNKHEAVITQKAQRYIRNN
                     YPSAEKKYPGNKDEQVLYKDTLDQLLRTRTQKLLDSTKDTKITWWGTTYQKSKENELL
                     LGLDLQGGINVTLDIALEGLVKNLANNPRDQTLLKTIQLADQKKLTSGGTNFIDLFAQ
                     AFKEINPTGRLAPLFANTNRNKLKYESSDADVIRYLHDQASDGMKQTYQVLRNRIDKF
                     GVAQPSINLDENKGIITVELAGATDPDRIRKYLQSTANLQFWAVYNIGELSNAFQNAD
                     KALQKYLNGVRDSATLAKTDTTADSTSSALNQNPLVRVIRFQQPSQDEKGKVHYPAAI
                     GVPLTKDTPLVNSYLNNPIVRNQFPSNLKFLWGKQQTDDNGKILPFLELYAIKTIPGT
                     DKAPLEGEVISDARQDYDPVSGGVVVEMSMYKHGADVWAKITGDNVGLPIAIVLDDIV
                     YSAPYVNGQITGGNSQISMGNKSSGQTVEEAHDLANILKSGKLNAPARIVQEQVVGPT
                     LGREAVHGGALAFGISFLVIFTLMLIYYNTAGWVANIALILNLLFTIGILSALHATLT
                     APGIAGLVLTIGMAVDTNVIIFERIKEELTRGKSYPIAVHDGYRRSLAPVLDAHVTTF
                     LTAAILAYFGLGPVLGFATTQMLGIVLSLFCGILVSRLVTDFYTNKNRHFKYFTGISK
                     KIFRHASFKFIEYRKYTYMISVVVLILGISAYFHGFKKGVEFLGGRSYIVKFNKPIKD
                     QAVRDSLNKQFGRYTEIKTYGSNEQLEITTDYLVEQEGSTADSIVINKMFAGLKSFLP
                     ESATAQEFTGTNKYIQQSKKVGASISDDLKAGAVKATIFALLIIFLYIFIRFRDWRYS
                     LGTIFSLLHDVLVTLAVFSFLPRFVPFPLEIDQHFIAAILTVIGFSMNDTVIVFDRIR
                     EDSRILRGKTKGEIINKAVNETLSRTVMTSLTVFLTILILFLVGGEVTRGFAFAMLIG
                     VITGTYSSIFVAAPILMDLSPKRPLGAADTHATTGVKPKPALTR"
     gene            7466..8290
                     /locus_tag="VGM24_06760"
     CDS             7466..8290
                     /locus_tag="VGM24_06760"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEY4155107.1"
                     /translation="MARSKLIHSLKSKISTDRDKFYLRLRAVLPQAIGDRCSAFPVLC
                     SVCCGIIFSLLTASCNKSSGVKENKAFVTVTHVAPGFSALNILSDGDSILGNLAYGQT
                     SGVPENPYVEATAGVRKLQVTEGSETVLEGNTAFQQGLHYSIFAYDSLKNDSLKLFIL
                     QDNVQIRTDTFTYTRFINFSPGSNLNLVLTSKRDTVATGFQPFAGNKLSPSYYSFRIL
                     HIGRYAARAFRDTLFANSIPLDSLVVDSTKIYTIFLQGFADSTGGTGLMLKSIRHN"
     gene            8361..>9270
                     /locus_tag="VGM24_06765"
     CDS             8361..>9270
                     /locus_tag="VGM24_06765"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY4155108.1"
                     /translation="MQLSSILSRFSEPETLKMAKLGRELRAQGIDIIDLSLGEPDFDT
                     PGHIKEAAKKAIDDNWSHYTPVSGYLDLREAICTKLKRDNNLDYKPDQVVASTGAKQT
                     LANAILALVDEGEEVIIPTPYWVTYSELVKIARGKVVEVHTSLESGFKITAKQLEAAI
                     TPKTKAFLFSSPCNPSGAVYSKEELSALADVFKKHPQIFILSDEIYEYINFVGKHESI
                     AQFTELKDRVVLINGLSKGFAMTGWRLGWLAASAEVAKACEKLQGQITSGTSSITQKA
                     GVTALTTDLRPSFAMVEEFGRRREKTM"
BASE COUNT         2250 a         2229 c         2344 g         2447 t
ORIGIN      
        1 gtagtcgccg tccatactcc cgccgttgag gtttaacacc cgctgggcct tctgcagggc
       61 ggaaagggcc tgattatagg attcccgggc agcaagctgg tcccgctgcg aggccagtcc
      121 gcttttgtac atatcgttag ccgcatcaag ggatttgcgg gccacttcca ggttggatct
      181 ggccagggtc aggtcattgc tgaatcctgc catttcagcg cttttcaaca ccgccagcac
      241 ctgccctttt tttacataat ctcccagcat cacgctgatc cgggaaacct gtccgctgac
      301 caccggataa atttttagta tccggtcttc atcgaaatcc accttaccgg tcagggtgag
      361 cgagttcaat accttggccg tgctaaccgt atcgatctcg atcgttttca gcagggaatc
      421 gggtaaaaca tagccctctt ttgtatctgt tgccgcggct ttttcggagc aggaagagat
      481 ggtcagggaa aataccaaaa ggagaaaaaa taaaatttta taaaggactt gcatattttt
      541 tggtaaacca gtttaaggat ggaagaactc cgttccggtg taaaagttga tatcttcaaa
      601 ggcgaccagc cggttaaaaa ggatattgtt cgaggccagg atattttctt tataggaatt
      661 atagaaatcg aggaaatcca gcagggtgat atttcttttt cggtaattca gcagcacctc
      721 atttaacatc cggtcgaaat cggctaaaaa tttcggatcg atatttttca gcattttatc
      781 attcgagaaa gccttctgga gtgagcggaa aacattttcc tccacagtgg cttccatatt
      841 ctgctgcaac accgtggagg cctcggtatt gagttttgcg gtcaggatat ttcctttgtt
      901 ccggttaaag aacgggaggt cgatagcagc ccccaggctg ctgaggttat tgatataact
      961 tccctgctga tcataagcca gggaaagggt gaggttggga acggcgaggg ctttctgata
     1021 attgtaattc agtttactga tatccacatt cgtccgggca atgagcaggt ccgaccggtt
     1081 cttgtacgca gaatccagca gtgtgtttaa cggataggcg gccggatcaa aagagcgcag
     1141 gacgctggta tccacctggg gaacaataaa agaatcctta acctgtaaga ccagcctcag
     1201 ttcgctttgc agatcattga tctggttgtt aaggtcgttg tattcattta agagggcata
     1261 cagctgggct tttacccgga taacctcttt ctccgcgata tatccccggc cttcctgttc
     1321 gttgaaggcg tcaaccacct gctgcagcga ttttatttcc tcatcatata ccgccgcgga
     1381 ttgtaacagg taatgaatat tgaaaaagtc cgtccgcagc gtatatttca gggttctgac
     1441 caggtcgtag aactgatagt acgcgagccg ggtgccggcc cgggcaatct tcagctgttt
     1501 attccgctgc cccgcgaggt agacgacctg ggatagctgg gcagccagtt cgccaccgct
     1561 ttccccgggc ccgaccggaa atattttctt cgtttttgta ttgtacaagc tgtaggatgc
     1621 cgaaagattt ggattcggat aaaggtgcgc ctggatctcc tgggcctgct gtgcctgtat
     1681 attatatttc agggcaagca gggaaaaatt cccggaaata aagagatgtt ccgcgctgtc
     1741 caggtttatg cggagggaat ctccgggaat ttgcgctttc ccggtaccgg taaaagaagc
     1801 gaaaattaat agggagatga atttttgtaa catgggttac gaggttattg aactacggcg
     1861 ttcaggcgga agccgatccc gtggatggtt tcgatattgg ctgagggatc tgctttaaaa
     1921 tatttccgga gcttggtgat gaacacatcc atgctcctgc ccagaaaata atcgtccttg
     1981 ccccagacat tcagcaagac ctcttcccgt ttcaggatcc ggttgggatg ttcgcacagg
     2041 aactggagaa gttcggcctc tttctgtgtc agattatgtt ccgttttgtc caccatcagt
     2101 ttcagattat taaaggagaa aagcatctcc ccgatccgga atgactggga cctttcggaa
     2161 tacatttttt ttgtcctgcg taaaaacact tcgatgcgga gcatcagttc ctgcatgctg
     2221 aagggcttga gtatataatc atctccgccg gattcaaaac catggatacg gtcctcttcc
     2281 agtgatttgg aagtaatgaa aaggatggga acgatgtccg tctgctgcct tatcttcttt
     2341 gccacggcaa atccgtcttt gttgggcatc atcacatcca gcaggcaaat gtcaaagctt
     2401 tctttattga atttatcgat cgccgtttgt ccgtcggggc aaagcaccac gtcataaccg
     2461 gcatcaacca ggttgtcctt gattacgtat ccgagggaaa ggtcatcctc ggcgagtaat
     2521 acttttgctt tattgctcat tcggtttatg ttagattcga tataaaatga ttaaaaagga
     2581 aggaggagcc tgaattcggt gcctatgccg ggcaagctgt tcacccgtat ttttccatca
     2641 tggccgtcaa cgatccgttt cacgaagttc agcccgagtc caaatccctt tacatcatgc
     2701 acatctcccg taggaacccg atagaatttt ttgaaaatat ttttgatgta ttttttttcg
     2761 ataccgatcc cgttgtcctt gaccgaaata aaataatccg aatcttcccg gccggcctgg
     2821 acgacaatat gcggcgagtt ggaatatttt aaggcatttt ccagcaggtt aaccacggcg
     2881 agttccaggt gggactcatc cccctggata aaagtctcat agttatccac tttcaattcc
     2941 accttggcct gtctttgttc gatcagcggc tgaagtttgt tcagggcctg ttgaatgaga
     3001 aaagccgggt tgatggtttg tttgtcaatg gggagctttt tgttttccac cgaagcggac
     3061 ttcagcagtt tttccacctg gctctgcagg tgcgtggttt ggttctggat aatcgatgca
     3121 taccgtttca gccggtccgg cttattgacg atgtcttcct ggcccagcac atcgccggca
     3181 attttaatta ccgcaagcgg cgtcttgaat tcgtgggtga aattattgac aaaatccttc
     3241 tggacttccg cgaggaattt ctgccggtaa aaataaaaca ggcttatggc caggctgacc
     3301 agggccaggg aaagggcgac gctgctgacg atccagaagt tcatctcgcc aagaatatac
     3361 agggagcgat ggggaaaata caggaggatg tgatcgtaat ccgtggtaaa taccggcagg
     3421 ccgctggtgc cggtaacatt gtaccgggaa gccgcggtag gtacatattc ctcatatata
     3481 tatttcttat tcagcttgct gtaagccccc agcttcacat cggtaagcac atcgaaatcg
     3541 ccgaattcct ttttcagata gtgggtgagg gagtctttct ccggaatggt atccgcctta
     3601 aacaggtagt agtcgtcctg cggattgtgg atgagctgct ccaggctcag ggaagggtcg
     3661 tcattcattt ccaggtcttc aaaaacaccc cggaggcttt tcactatatt ggtgctgaac
     3721 tgtttttttt caaaggaata gaccttcgtg agccagaata cctgaacgat cacaatcagg
     3781 ccaataacaa ttgtggccga aagcaccagc cattttaaca ttgtggatcg catggaccgt
     3841 aggtaaaaat aagaacgcaa attgggaaag attgtgttgt gctaaattac aaaattggtt
     3901 aaacaccggt taacgacctg aatttcagcg tgaacagcac aataaaccag aatttggttt
     3961 tgaattgatt ttatctattt ttataacagc ctgtgaaaaa cggaacgacc ggcaggcctg
     4021 acaggagaac gtattattgt taaattaaac tcagaacaat catgaaaagt atccttttag
     4081 tgttgggact tttatttttt tcccttgctt ccttcagcca accgcaccat ccgccgcgcc
     4141 cgcaccatcc ggtgaggcat catcatgcgc cgcaccatgt gaagcaccgg cctgcgcatc
     4201 atccggttca tcacgcgcca caccgctgat caggcaaaac aaacggatta ttaagcaaaa
     4261 accctccgaa agcggagggt tttttattca aagagatcgg aaaatctatc tggtcaaagc
     4321 aggttttggt tttacgcctg tcgtagcatg cgtatctgct gcgccgaggg gcctttttgg
     4381 tgaaaggtcc atcaggatcg gagcggccac aaaaatggaa gaataggttc cggtaattac
     4441 cccgatgagc atggcaaagg caaatccccg ggttacttca ccgcctacga ggaacaggat
     4501 caggatggta aggaatacgg tgagagaagt cattaccgtt ctgctcaggg tttcatttac
     4561 tgctttgtta atgatctctc cctttgtttt accccggagt atccggctgt cttcgcggat
     4621 cctgtcgaac acaataacgg tatcgttcat ggagaacccg atgaccgtaa ggatggccgc
     4681 aatgaaatgc tggtctattt ccagcgggaa aggaacaaag cggggcagga aggagaacac
     4741 ggccagggtg accaggacgt catgcagcag ggaaaagatg gttcccagcg aatatctcca
     4801 gtcgcggaag cgaatgaata tatacaggaa tatgatcagc agggcaaaga tcgttgcctt
     4861 tacggctcct gctttcaggt cgtcggaaat agacgccccg actttcttcg actgctgaat
     4921 gtatttgttg gtgccggtaa attcctgtgc cgtagcgctt tccgggagaa atgatttcag
     4981 gccggcaaac attttattga tcacaatgga atcggcagtc gatccttcct gttccaccag
     5041 gtaatccgtt gtgatctcca gctgttcgtt ggagccatag gtttttatct cggtgtatct
     5101 tccgaactgt ttattcagag aatcccggac cgcctgatcc ttgatgggct tgttgaactt
     5161 tacgatatag ctccgtccgc ccaggaattc cacccctttt ttaaatccat gaaaataggc
     5221 ggatataccg aggatcagca cgacgacgga gatcatgtag gtatacttcc ggtattcgat
     5281 gaatttgaaa ctggcatgcc ggaatatttt tttcgagatg ccggtgaaat atttgaagtg
     5341 gcggttttta ttggtataga aatcggtgac gagccgggag accaggattc cgcagaaaag
     5401 ggaaagcacg atacccagca tttgggtggt ggcgaatcca agcaccggcc caagcccgaa
     5461 ataggccagg atggctgccg taaggaaggt cgttacgtgg gcatccagta ccggggcaag
     5521 cgatcgccgg taaccgtcgt gcaccgcaat gggataactc tttcctctcg ttagttcttc
     5581 cttgatgcgt tcgaatatga tcacattcgt atccacggcc atgccgatcg tgagcaccag
     5641 accggctata ccgggcgccg ttaacgtggc gtgaagggcg ctcaggatcc cgatggtaaa
     5701 gagcaggttc agaatcaggg caatgttcgc cacccatccg gctgtattat aatagatcag
     5761 catgagggtg aagatgacga ggaaagaaat gccaaaagcc agggccccgc catggacggc
     5821 ttctcttccg agggttggtc caacaacctg ttcctgtacg atgcgggcgg gcgcattcag
     5881 cttgcccgac ttcaatatat tggccaggtc atgcgcttct tcaacggttt gcccggagct
     5941 tttattcccc atgctgatct gggaatttcc gccggtgatc tgtccgttca cataaggcgc
     6001 cgaataaacg atatcatcca gcacaatggc aatcggtagc ccgacattgt cgcccgttat
     6061 ttttgcccag acatccgctc catgtttata catgctcatt tccacgacta cgcccccgct
     6121 caccgggtca tagtcctggc gggcatcgga gatcacttct ccttccagcg gcgccttatc
     6181 ggtgccggga atggttttga tggcatagag ctccaggaaa ggaaggattt tgccgttgtc
     6241 atccgtttgc tgctttcccc agaggaattt caggttggaa ggaaattgat tacgaacgat
     6301 ggggttattg agatagctgt ttaccagcgg agtgtccttt gtcagcggaa cgccgatagc
     6361 ggcgggataa tggacctttc ctttttcatc ctgtgaaggc tgctgaaaac ggatcacgcg
     6421 caccagcgga ttttgattca gcgcactgct ggtgctgtct gccgtggtat ctgtttttgc
     6481 cagcgtggcg gaatccctca cgccgtttaa atatttctga agcgccttgt cggcattttg
     6541 aaatgcattg gataattcac caatattata tacagcccag aattgcagat tggcggtgga
     6601 ctgcaggtat ttccggatac ggtccgggtc ggtggctccg gcgagttcaa ccgtgataat
     6661 gcccttgttt tcatccaggt tgatactggg ttgggcgacc ccgaatttat caatacggtt
     6721 tctaaggacc tggtaggttt gcttcatgcc atctgaagcc tgatcgtgca ggtagcggat
     6781 cacgtctgcg tcggaagatt cgtatttgag tttattgcgg ttggtgttgg cgaaaagggg
     6841 cgccagcctc ccggtgggat ttatttcttt aaatgcctgg gcaaaaaggt cgataaaatt
     6901 ggtgccgccg ctggtcagtt ttttctgatc cgccagctga atggtcttca gcagggtttg
     6961 atcgcgggga ttgttggcga ggttcttaac aaggccttcc agggcaatat cgagcgttac
     7021 attgatgccg ccctgaagat cgagccccag gaggagttcg ttctctttgg atttctggta
     7081 ggtggttccc caccaggtga tctttgtatc ttttgtgctg tcaagtaatt tctgggtacg
     7141 tgtacggagc aactggtcga gcgtatcttt gtaaagcacc tgctcgtctt tatttccggg
     7201 atattttttt tctgctgaag ggtagttatt ccggatatac cgctgcgctt tctgggtgat
     7261 taccgcttcg tgcttattca caaaccaggt aaaggacagt tggtataggg aaatgacaat
     7321 cagtaagccg gcaaacatgc taaccagggc tctcatcggt tttcaatttt ttaagagcgg
     7381 caaagatagg ttttacaacg gataattaaa atgttaagac cttacccgga aaagccgggt
     7441 tttcctactt tcacgcctga taattatggc caggtcaaaa ttaatccatt cattgaagtc
     7501 gaagatcagc acggaccggg ataaattcta cctccggctt cgcgccgttc tcccccaggc
     7561 gatcggggac aggtgctccg cattccccgt tctttgttcc gtttgctgcg gaatcatttt
     7621 ctccctgctt accgcctcct gtaataaatc ctccggtgtc aaggagaata aggcgtttgt
     7681 gacggtaacc catgtggctc ccggattttc agccttgaat atcctttccg acggggactc
     7741 catacttgga aacctggcat acgggcagac ctccggggtc ccggaaaacc cttacgttga
     7801 ggctacagca ggcgttcgta aactgcaggt tacggaaggg tccgaaaccg tgctcgaggg
     7861 aaatacggct tttcagcagg gacttcatta tagtatattc gcgtacgatt ccctgaaaaa
     7921 cgactccctg aaactattca tcctccagga taatgtgcag atccggacag acacttttac
     7981 ttatacccgg tttatcaatt tctctcccgg gtccaatctc aacctggtgc tgaccagtaa
     8041 gcgggacacg gtcgccacgg gctttcagcc ctttgccgga aataaattaa gtccttccta
     8101 ctattccttc cgtatcctgc atatcggcag gtatgccgcc cgcgcgttcc gcgacaccct
     8161 ttttgccaat tccattcccc tggattcgct tgtggtggac agcaccaaaa tctatacgat
     8221 cttcctgcag gggtttgccg attcaacggg cggcaccggc ctgatgctga agagcataag
     8281 gcataattag ggtcaatccg ccgccatccc ggttcgcatt aatcccaaaa tatatacctt
     8341 tgcgcccgca actaaataac atgcaattat cttctattct ttcccgtttc agcgagcccg
     8401 aaaccctgaa aatggccaaa cttggccgtg agctcagggc ccaggggata gatattatcg
     8461 atttgagtct tggcgaaccg gactttgata cacccggtca cattaaagag gctgccaaaa
     8521 aagccattga cgataactgg agccactata caccggtttc cggatacctg gatctccgcg
     8581 aagcgatctg caccaaactg aaaagagaca ataacctcga ttataaaccg gatcaggtgg
     8641 ttgcttccac cggggctaaa caaaccctgg ccaacgcgat cctcgccctg gttgacgaag
     8701 gggaggaagt gatcatcccc accccgtact gggtgacgta ttccgaactg gtaaaaattg
     8761 cgcgcgggaa agtggtggaa gtgcatactt ccctggaatc cggattcaag atcaccgcaa
     8821 aacagctgga agcggccatt acacccaaaa caaaggcatt ccttttttct tccccctgca
     8881 atccgtccgg cgccgtttac agtaaagaag agcttagcgc gctggcggac gttttcaaaa
     8941 aacacccgca gatctttatc ctctcggatg aaatttacga gtacatcaat tttgtcggca
     9001 aacacgaaag catcgcacag tttaccgaac tgaaagaccg ggtggtgctg atcaacggat
     9061 tgagcaaggg ttttgccatg accggatgga gactcggctg gctggctgct tctgcggaag
     9121 tcgccaaagc ctgtgaaaaa ctacagggtc agattaccag cgggaccagt tcgatcaccc
     9181 agaaagccgg cgtgacggcg ctcacgacgg acctccggcc ttcctttgcg atggtggagg
     9241 aattcggcag gagaagggag aagaccatgg
//
LOCUS       DASYCU010000334         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2246919, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000334 DASYCU010000000
VERSION     DASYCU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246919"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /gene="metG"
                     /locus_tag="VGM24_06770"
     CDS             complement(<1..1910)
                     /gene="metG"
                     /locus_tag="VGM24_06770"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY4155109.1"
                     /translation="MKEYNRILVTAALPYANGPIHIGHLAGCYIPADIYARYERARKK
                     NILFVGGSDEHGVPITIRAMKEGISPQQVVDKYHALIRESFEQMHISFDIYSRTTNPI
                     HHKTAADFFKTLYDEQVFEEKETEQYYDEKAKLFLADRYITGTCPICGNPNAFGDQCE
                     RCGSSLSPEQLINPRSTLSDAVPVKRKTTHWYFPLQKYEEFLKEWILNGHKEWKNNVY
                     GQCKSWLDNGLQSRAMTRDSNWGVKVPLPDAEGKVLYVWFDAPIGYISATKELTPNWA
                     DYWCQPDTKLVHFIGKDNIVFHCIIFPAMLKAHGKFVLPDNVPANEFLNIEGEKVSTS
                     RNWAVWVHEYLRDFPGCEDVLRYVLCANAPETKDNDFTWKDFQDRNNSELVSIFGNFV
                     NRTFVLMHKLCRGKVPVWHPAIEDGADRELVIQIRETKAAVENLLEQYKFRDSLFAII
                     DLARKGNKYLQDKEPWKKAGKENTSPENQQLIDNCLYLCLQLTANLSILINPFLPITA
                     KKMLHMMKVVDRMLDWEHAGKTDLLKTGYSLRAPELLFRKIEDDEIQAQMEKLHQNST
                     KPNPETKEEPAVVVPEKKPEIQYADFAKLDLKAGIITQAEKVPKADKLIRLEVDLGSE
                     KRTIVSGIAMHF"
     gene            2066..3100
                     /locus_tag="VGM24_06775"
     CDS             2066..3100
                     /locus_tag="VGM24_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY4155110.1"
                     /translation="MKRKHFLSSLAASPLIPALSFRSPFGAPAWRMPEKEGFAIPPYL
                     KPGDSIGITCPAGHMLAEELEPALAALQSWGFKLRLGSTVGKRDFTFGGTDEERRADL
                     QQMLDDPSLQAILCARGGYGCIRIIDQLDFRQFVAKPKWIVGFSDITVIHAHLNHNYR
                     IASIHSKMCNSFPYRMNEIEEEQRNSILSIRTALSGDRVEYPVSADPGNKNGTGTGLL
                     AGGNLKTIETLAGTASDLRTDGMILFVEDVGEYLYSIDRMFYSLKRTGKLDRLSGLLV
                     GGFKVKPDDPGDEFGRSVYNIVLDQVSPYSYPVCFNFPVGHQKVNYALKCGVMHQLTV
                     SGQEVIFKEV"
     gene            3102..4019
                     /locus_tag="VGM24_06780"
     CDS             3102..4019
                     /locus_tag="VGM24_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY4155111.1"
                     /translation="MSEIPPYLSPGDTIGMVCPAGYMAAENTVQCRTTLEQWGFRVKT
                     GKTIGGKSANYFSGTDEERLDDLQEMLDDESVKAILFGRGGYGVGRIIDRISFKRFRK
                     TPKWIMGYSDITLLHAHIYANYGIATAHSPMAAAFNDPQQPNPYTQSIYDLLTGERYS
                     RTCDAHAFNRKGETKGILIGGNLTLLAHAVGGPSDIKTRGSLLFLEDIGEYLYNIDRM
                     MYQLKRAGKLHKPEGIIIGSFTDCKDTERPFGKSAYEIIRDILDEFDYPVCFGFPVGH
                     DTENYALKCGVEYKLKVTNSKVTLVEADN"
     gene            4081..>4187
                     /locus_tag="VGM24_06785"
     CDS             4081..>4187
                     /locus_tag="VGM24_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY4155112.1"
                     /translation="MTFTQLEYILAIDAHRHFAEAADACFVTQPTLSMQV"
BASE COUNT          964 a         1083 c         1058 g         1082 t
ORIGIN      
        1 tcaaagtgca tcgcaatccc ggatacgatg gttctttttt cagatcccag gtccacttcc
       61 agccggatca gtttatccgc tttgggcact ttttccgcct gcgtgatgat gccggccttt
      121 aaatccagtt tggcaaaatc ggcatactga atctccggtt tcttttccgg aacaaccacc
      181 gcaggttcct cctttgtctc cggattgggt tttgtcgaat tctgatgcag tttctccatc
      241 tgtgcctgta tttcgtcgtc ctcgatcttc cgaaagagca gttccggcgc gcgaagactg
      301 tatcccgttt tcagcagatc cgtttttcca gcatgctccc agtccagcat ccggtccacg
      361 accttcatca tgtgtagcat tttttttgcc gtgatgggaa gaaaggggtt gatcagtatg
      421 gacaggttcg cggtgagttg caggcagaga taaagacagt tatctatcag ctgctgattt
      481 tcagggctgg tattttcctt tcctgctttt ttccagggct ctttgtcctg cagatattta
      541 tttcctttcc gcgccagatc gatgatcgca aataaggaat cccggaattt atattgttcc
      601 agcaggttct ccactgccgc ttttgtttcc cggatctgga tgaccagctc ccggtcggcc
      661 ccgtcttcaa tcgccgggtg ccagaccgga acttttccac gacagagttt atgcatcagc
      721 acgaatgtcc ggttcacaaa attgccgaag atggatacca gctcgctgtt gttccggtcc
      781 tgaaaatcct tccaggtaaa atcattgtcc ttggtttccg gcgcattggc gcagagtaca
      841 tatctcaaca cgtcttcgca tccggggaaa tcccggagat attcatgaac ccagaccgcc
      901 cagttccggc tggtggatac tttttctcct tcgatattta aaaattcatt tgccggcaca
      961 ttgtccggca gcacaaattt tccatgcgct ttcagcatgg cagggaaaat gatgcaatga
     1021 aaaacgatat tgtcctttcc gataaagtga acgagcttgg tatcgggctg gcaccagtag
     1081 tcggcccagt tcggggtgag ctccttggtg gccgaaatat aaccgatggg cgcatcaaac
     1141 cacacgtaaa gcactttccc ttccgcatcc gggagcggca cttttacccc ccagttactg
     1201 tccctcgtca tcgcgcggct ttgcaagcca ttatccagcc agctcttaca ctggccgtac
     1261 acgttatttt tccattcttt atgtccgttg aggatccatt ccttgagaaa ttcctcatat
     1321 ttctgcagcg ggaaatacca atgcgtggtt tttcttttta caggaaccgc gtcactcagg
     1381 gtgctccgcg gattaatcag ctgttcggga cttaaggaag aaccgcagcg ttcgcactgg
     1441 tcgccgaaag catttggatt cccgcaaatg ggacaggtgc cggttatata gcggtccgcc
     1501 aggaaaagtt tcgccttttc atcgtaatac tgttcggttt ctttttcttc gaaaacctgt
     1561 tcatcgtata aggtcttaaa aaaatcagcg gcggttttat gatgaatggg attggttgtt
     1621 ctggaataga tgtcgaagga aatatgcatc tgttcaaaac tttccctgat cagggcatgg
     1681 tacttgtcaa ccacctgctg cgggctgatc ccttctttca tcgcccggat ggtaatgggc
     1741 acgccatgct cgtcgcttcc gccgacaaaa agaatgtttt tctttcttgc ccgttcatac
     1801 cgtgcataga tgtccgcggg gatataacag cccgccaggt gaccaatatg aatggggccg
     1861 tttgcatagg gaagggcagc ggtaacgaga atccggttgt attctttcat agtttaaaat
     1921 agtcggccgc aaaggcgccg ttcagcctgc aaaaatacca attcaaatcc attgtcccgt
     1981 tttgaatccc cgcacccgcg ccggagtcct ttgtatggct taacctgctc cttggcaatt
     2041 cccgttcatc cgttaacttt aattgatgaa gcgtaagcat tttctttcct ccctggctgc
     2101 cagtcccctg atcccggctc tttcttttcg atctcctttc ggggcgccgg cctggcgaat
     2161 gccggagaag gaggggtttg cgatcccgcc ctacctgaag cccggagaca gcatcggaat
     2221 aacctgtcct gccggacaca tgcttgctga agaactggag cctgccctgg cggccctgca
     2281 aagctggggg tttaaactgc ggttaggcag caccgtgggg aagcgggatt ttactttcgg
     2341 cggaacggac gaagagcgcc gggccgatct gcagcagatg ctggatgacc cttccctgca
     2401 ggccatctta tgcgcccggg gcggctatgg ttgcatccgg atcatcgacc agctggattt
     2461 caggcaattt gttgctaaac ccaagtggat cgttggtttc agcgacatca ccgtcatcca
     2521 cgcacacctc aatcacaatt accggatcgc ttcgatccac tccaaaatgt gcaatagctt
     2581 tccttaccgg atgaatgaga tcgaagagga acagcggaac agtatcctgt ctatccggac
     2641 ggcccttagc ggagaccggg tggaataccc ggtttcagcg gacccgggaa ataagaacgg
     2701 taccggcacc ggattactgg ccggcggaaa cctgaaaacg attgaaacgc tggccggcac
     2761 ggcctccgac ctccggaccg acgggatgat cctgttcgtg gaggatgtgg gcgaatacct
     2821 ctacagcatc gatcgcatgt tttacagtct gaaaagaacc gggaaactgg accgcctgag
     2881 cggtttgctg gttggcggct ttaaagtaaa acccgatgac ccgggagatg aattcggaag
     2941 aagtgtttac aatattgtgc tggatcaggt gtcgccctat tcttacccgg tctgttttaa
     3001 tttcccggtc ggccaccaga aagtcaatta tgcgttgaaa tgcggggtta tgcatcagct
     3061 gacggtcagc ggacaggaag ttatttttaa agaagtttaa tatgtcagaa attcctcctt
     3121 acttatcccc cggagatacc atcggaatgg tttgcccggc gggttatatg gctgccgaaa
     3181 atacggttca atgcagaacc accctggagc aatggggatt ccgggttaaa acgggtaaaa
     3241 ccattggcgg gaagtccgct aattattttt ccggtacgga tgaagaaagg ctggacgacc
     3301 tgcaggagat gctcgatgac gaatcggtaa aagccatatt gttcggccgc ggcgggtatg
     3361 gggtggggcg cattatagac cggatcagtt tcaagcgctt ccggaaaacc cccaagtgga
     3421 tcatgggata cagcgatatc actctgttgc atgcacatat ctatgccaac tacggcatcg
     3481 ccaccgcgca ttcccccatg gctgccgcct tcaacgaccc gcagcaaccc aacccctata
     3541 cccaatccat ttatgatctg ctgacgggtg aaaggtattc ccggacttgc gatgcccatg
     3601 cgttcaaccg gaagggcgaa acaaaaggca tattgattgg aggcaacctg accctgcttg
     3661 cccacgcggt gggcggacct tcggatatca aaacccgggg atccctgctt tttctggaag
     3721 acatcgggga atacttatat aatattgacc ggatgatgta tcagttgaaa agagctggaa
     3781 aattgcataa gccggaaggg atcatcatcg gcagttttac ggactgcaaa gacacggagc
     3841 ggcccttcgg aaaaagcgcc tacgaaatca tccgcgatat cctggatgaa tttgattatc
     3901 ccgtctgttt cggatttccc gtcgggcatg acacggagaa ctatgccctg aaatgcgggg
     3961 tggaatataa actgaaagtc acgaacagca aggtgacgct ggtggaagcg gacaactgat
     4021 ccttttattc atcgaccaaa ttgattaatt ttataatccg atcgatttgg ttgatatcgt
     4081 atgacattta cccaactgga atatatcctg gccattgatg cgcaccggca cttcgccgag
     4141 gccgccgatg cctgctttgt tacgcagccc acgctcagca tgcaggt
//
LOCUS       DASYCU010000335         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_362373, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000335 DASYCU010000000
VERSION     DASYCU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362373"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..847)
                     /locus_tag="VGM24_06790"
     CDS             complement(89..847)
                     /locus_tag="VGM24_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY4155113.1"
                     /translation="MTCFCFCSETVYGQADSIRADTVFPAQDSIAETDTAVLDQTDFP
                     VTDSAAASEHLRRVPDSRVKAYQRDPDFAYANDPAYWKELPPPHSRLGEKFFRFLFSR
                     GFRIGLFILFLLLAGYGLYRLSKENSFTWFRRSSRPDHTAGPAGAAEDPEETDLDEAI
                     RKNREAGNYKLAIRYMYLKIIRAAFSKGAILIKASSTNSDITRAFRDPQQARDFRYLA
                     TAYEHVFYGEFMPDREQFDLLEKKFDLFNQNLEN"
     gene            complement(900..1865)
                     /locus_tag="VGM24_06795"
     CDS             complement(900..1865)
                     /locus_tag="VGM24_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEY4155114.1"
                     /translation="MREGQFIQQNIEKWKTYQYEPTSDPDEMAQRFTELVNDLGYSKT
                     FYPQSKVTEYLNRLASGIYLGIYKNKKEESSRIIRFWKSELPLVVRKHHRELIYSFAI
                     FLIFALMAAFSASHDEHFVRGVLGDRYVEMTEENIAKGDPFGVYKSQNALSMFLWIAV
                     HNIQVSFLIFVSGIFACLGTVWLLFTNGVMLGSFQYYFFAKGLGWKSVLVIWIHGTLE
                     ISSFIIAGAAGILLGKSLLFPGTFKRLPSLKQGARDGVKLMIGLVPVFILAAFLEGFV
                     TRYSGMPVWLSLFILIVSLLFIVWYFVIYPIRLSRKLTLPEYIKF"
     gene            1903..>2586
                     /locus_tag="VGM24_06800"
     CDS             1903..>2586
                     /locus_tag="VGM24_06800"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY4155115.1"
                     /translation="MLSVKLDTGFNIEVVFPVSPFHRRMFAWLIDILLMFVYGLLCQK
                     LFGKLPENKTENAEWLWVLIGLPVLFYYPVMEILTNGQTVGKKLLGIRVITAEGGQAT
                     VSQYLLRWIFRTADFPVWIFAAIFTGALPWYCSILTFSGVGCVIMSHKSQRIGDIIAG
                     TIVIFTRSKTSWEDTVFTEVEAGYKPRYPEVMRLSDKDLNSLKTIIGAVRKSSNHEIA
                     LRISERIKGK"
BASE COUNT          712 a          632 c          613 g          629 t
ORIGIN      
        1 ttattcaatt tcttttggcg tgtacaggat gaacaggaac cgagtgcggc caggcagaaa
       61 ataaaaatga tatgccgggt aagcaatttc aattctccag attttggtta aacaggtcaa
      121 atttcttttc cagtagatca aactgctccc ggtccggcat aaattcccca tagaaaacat
      181 gctcataagc ggtggccagg taccggaaat cccttgcctg ctgcggatcc cggaaggccc
      241 tggtgatatc tgaattggtg gaagaagcct tgatcagtat ggcgcctttg ctgaaggccg
      301 cccggataat tttcaaatac atatagcgga tagccagttt gtagttcccc gcctcccggt
      361 tttttcgtat ggcttcgtcc agatcggtct cttcaggatc ttctgctgcc ccggccggtc
      421 ccgcggtatg atcgggcctg ctgcttcgcc tgaaccaggt aaaagaattt tctttagaca
      481 accggtaaag gccataaccg gccagcaaaa gaaataaaat aaaaaggccg atgcgaaaac
      541 cccggctaaa caaaaaccgg aaaaattttt ctcccaggcg ggagtgcggc ggaggcaact
      601 ccttccagta tgccgggtca ttggcgtaag caaaatccgg atcacgctga taggctttta
      661 cacggctgtc cggaacccgg cgcaaatgtt ctgaagcggc tgcgctatcc gttacgggaa
      721 aatcagtctg gtccagaaca gccgtatcgg tttccgcaat ggaatcctgc gccgggaaca
      781 ccgtatctgc ccggatactg tccgcctgac cataaaccgt ttcgctacaa aagcagaaac
      841 aggtcaaaaa caatatgaca ttagtaaata gtttgagcat ttggatccgg ccagcggcct
      901 cagaatttaa tgtattcagg taaagtcagt ttccggctta gccggatcgg atatataaca
      961 aaataccaga cgataaacag cagcgaaaca atcaggataa agaggctgag ccataccggc
     1021 atgccggaat accgagtaac aaatccttcc aggaaagcag ccaggataaa tacgggcacc
     1081 agcccgatca tcagttttac tccatccctt gccccctgtt tcagggacgg cagccttttg
     1141 aaggttcccg gaaacaagag gctttttccg agcaggatgc ccgctgcgcc tgcgatgata
     1201 aaggaggaaa tttccagggt gccgtgtatc cagatcacca aaaccgattt ccatccaagg
     1261 cctttcgcga aaaaataata ctggaaagag cccagcataa ccccgtttgt aaataataac
     1321 caaaccgtgc ccaggcaggc aaaaattccc gaaacaaaaa tgagaaagga aacctggata
     1381 ttatgtacgg caatccatag gaacatagac agggcattct gacttttata aacgccaaag
     1441 ggatcaccct tcgcaatatt ctcttccgtc atttcaacgt agcggtcacc cagtaccccc
     1501 cggacaaaat gctcatcatg agaagccgag aaggcagcca tcagggcgaa gatcagaaat
     1561 atggcaaacg aataaatcaa ctcgcggtga tgttttctga cgaccagggg cagttccgat
     1621 ttccagaacc ggatgatgcg ggaagattcc tcttttttat ttttatagat gcccagataa
     1681 attccggatg ccagtctgtt caggtattcg gtaaccttac tttgcggata aaaagttttt
     1741 gaatacccca ggtcgttcac cagttccgta aaccgctgcg ccatttcatc cggatcggag
     1801 gttggttcat attgataggt tttccacttc tcaatattct gttgtatgaa ttgaccttcc
     1861 cgcacaatta gattatattt attgataaat ataacatctg ttatgttatc cgtaaaactg
     1921 gataccggat tcaatatcga ggttgtgttt cccgtaagcc cgttccatcg ccggatgttt
     1981 gcgtggctga tcgatatcct tttgatgttc gtgtacggat tattgtgcca aaaactattt
     2041 gggaaacttc cggaaaataa aacggaaaat gcagaatggc tttgggtatt gatcggtctt
     2101 cccgtccttt tttattaccc ggtgatggaa atactgacca acggacaaac cgttggcaag
     2161 aaattactgg gcatccgggt gatcaccgcc gagggcgggc aggctacggt aagccagtat
     2221 ctcctgcgct ggattttccg gaccgccgat tttccggtct ggatctttgc ggccatattc
     2281 acgggcgctc tgccctggta ctgcagcata ctcacctttt ccggcgtcgg ctgcgtgatc
     2341 atgtcgcata aatcccagcg catcggagat atcattgccg gcaccatcgt cattttcacg
     2401 cgttcaaaaa cttcctggga ggacacggta tttaccgagg tcgaagcggg ttacaaaccc
     2461 cgttaccccg aagtgatgcg gctgagcgac aaagacctca attcattgaa aacgattatc
     2521 ggggcagtga ggaaaagcag taatcacgaa atcgcccttc ggatttcgga aagaataaaa
     2581 gggaag
//
LOCUS       DASYCU010000336         4385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3842267, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000336 DASYCU010000000
VERSION     DASYCU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3842267"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1731)
                     /locus_tag="VGM24_06805"
     CDS             complement(49..1731)
                     /locus_tag="VGM24_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY4155116.1"
                     /translation="MISEEKNKQIQELIATLEQSDLIWMIGFLQGVLSKMPENANPVA
                     KPAASGKKITIAYGTESGNSKKVASVFAARAKQEGIQTKVVSLDQYRLADLAKEEYFL
                     TVISTQGEGEPPESAKKFYDHIHQNGFKLPKLKYGVLALGDTSYPMFCKAGEDVDQQL
                     DHLGAERIIDIQRCDVDYENDAQSWFNGVLQKLSGAKAPSVPAVKPVKKTSGKKIYSG
                     KILTNINLNDRGSAKHTHHIEIASEEVQYKSGDSIGIIPHNPEHLVKAIIEQTGINPE
                     ITFVHRDEACSAYELLHRKLNIVYLPERVVTKYAAHIQKEISPVKTGLLELLTQHPVK
                     DAAQFETFIQQLEPTTPRLYSIASSLEKFDGEIHLTVGRDEFCIAEERKYGLCSDLLC
                     SLKPGDQIDFYVHHNDQFRLPEEDKDVIMIGPGTGIAPFRAFLAEREATGAAGRNWLF
                     FGDQHFTTDFLYQTEIQQWLEDGVLHKLDLAFSRDQRGKIYVQDKIKKNASEFYNWLK
                     AGATVYICGAKRMSEDVEETILDIIRNSGETEDAEAYFNQLKESGRYLKDVY"
     gene            complement(1728..2552)
                     /gene="cobA"
                     /locus_tag="VGM24_06810"
     CDS             complement(1728..2552)
                     /gene="cobA"
                     /locus_tag="VGM24_06810"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEY4155117.1"
                     /translation="MNQNKNLPKLIIAGAGPGDPELITVKTARHLREAEVVITDRLVS
                     EVILEKYVSKSALVLHAGKQRGKEGSTPQHIINELLVEYALQGKRVVRLKGGDVSVFS
                     NLLDELQALNRYRIPYEIVPGITAACGAAAYAGIPLTARNYSNAVRLLTLHKTGLIEP
                     EYWKELAGTEDTLVFYMSGDLSDELADNLLRNGIKSDRHIAVIEQATTPMQRVYSCPL
                     NEYANRPGNRNYLSPTILIIGKVAALHEQFRWRPDNGSDELYFKPIKKTQQQEERA"
     gene            complement(2576..3820)
                     /locus_tag="VGM24_06815"
     CDS             complement(2576..3820)
                     /locus_tag="VGM24_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY4155118.1"
                     /translation="MDLLRFITAGSVDDGKSTLIGRLLYDSKSILTDQLEALEKQRKH
                     KEEESIDLALLTDGLRAEREQGITIDVAYKYFSTPKRKFIIADAPGHTQYTRNMVTGA
                     SNAQLMIILVDARNGIVEQTRRHSIIASLLNMPHVVVAVNKMDLVEYSQDVYNNIVIE
                     YASFARSLGLKNVQYLPISALNGDNIVDLSVNTPWYEGLPLLEILETTSISSDINLTH
                     ARLFVQYVIRPQSEELHDYRGYAGKIISGVYRKGDEIIILPAGINTRLARIERNQQEV
                     EEAVAGQSVILHLEDDIDVSRGDVLVKKDFPPLLGQELEVLLCWMDSKPLKAGNRYFL
                     QHNSRRVKSVVREIEYKLDVNSLEKQEGVEKAELNDIVRAKIKTASPLAYDLYKDLRA
                     NGGLILIDETSNVTVGACMIQE"
     gene            complement(3838..>4385)
                     /gene="cysD"
                     /locus_tag="VGM24_06820"
     CDS             complement(3838..>4385)
                     /gene="cysD"
                     /locus_tag="VGM24_06820"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY4155119.1"
                     /translation="TIEEFGFDACIGGARRDEEKARAKERIFSVRDEFGQWDPKRQRP
                     ELWNIYNGKIQKGENVRVFPISNWTELDIWNYIRRENITLPSIYFSHHRDVIEYEGQL
                     VAVSAFIRLDENDKVVNKKVRYRTVGDMSCTAAVESSASTVNDIINEIIATRTSERGE
                     TRIDDRLSEAAMEDRKKNGYF"
BASE COUNT          891 a         1130 c         1121 g         1243 t
ORIGIN      
        1 actcatgtct tcgttgttgg atccttggtt cgcttgtatt ttttttcctc aataaacgtc
       61 cttcagatat cttccggatt cctttaactg attgaaatag gcttccgcat cttccgtttc
      121 tcccgaattc cggataatat ccaggatggt ttcttccacg tcctcgctca ttctttttgc
      181 tccgcagata taaacggtgg ccccggcttt taaccagtta taaaattccg aggcgttctt
      241 ttttatttta tcctggacat atatttttcc gcgttgatcg cgggagaagg ccaggtcgag
      301 tttatgcaga acgccgtctt ccagccactg ctggatttcc gtctggtaca gaaaatcggt
      361 ggtgaaatgc tggtctccaa agaacagcca gtttcttccc gcagcgccgg ttgcttcccg
      421 ctcggcgaga aaggcgcgga agggagcaat gccggtgccc ggaccgatca tgatcacatc
      481 tttatcttct tccggcaagc ggaactggtc gttgtggtgg acgtaaaaat caatctgatc
      541 ccccggtttt aaagagcaga gcaggtcgga gcataaacca tatttccttt cttcggcgat
      601 gcaaaattca tcgcggccga ccgtcaaatg gatttcgcca tcaaattttt ccagggaaga
      661 agcgatggaa tacagccggg gagtggttgg ctccagctgc tgtatgaacg tttcaaactg
      721 ggcagcgtcc tttaccgggt gttgggtgag cagttccagc aggccggtct taacgggcga
      781 tatctctttt tggatatggg ccgcgtattt ggtcaccacc cgttccggga ggtaaacgat
      841 attcagtttc cggtgaagga gctcataggc ggagcaggct tcatcccggt gtacaaacgt
      901 gatttccgga ttgatgccgg tctgttctat gatggctttc acgaggtgct ccggattatg
      961 cggaatgata ccgatcgagt ccccggattt gtattgaacc tcctcggaag caatttcaat
     1021 atggtgggta tgtttcgcag aaccccggtc gttcagattg atattggtca ggatctttcc
     1081 ggaatagatc ttttttccgg atgttttttt gacaggcttc actgccggca cgctgggggc
     1141 tttggcgccg cttaatttct ggagaacccc gttaaaccag ctttgggcgt cgttttcata
     1201 atccacgtcg cagcgttgaa tatcgatgat gcgttcggcg ccgagatggt ccagctgctg
     1261 gtccacgtcc tcaccggctt tacagaacat gggataggag gtgtctccca gggccaggac
     1321 cccgtatttt aatttgggca gtttgaaacc gttctggtgt atgtggtcat aaaatttttt
     1381 ggccgattca ggcggctcgc cttctccctg ggtactgatg accgttagaa agtattcttc
     1441 ttttgccagg tcggccaggc ggtactgatc caggctgacc acttttgtct gaatgccttc
     1501 ctgttttgcc cgggcggcaa atacgctggc cactttcttg gagttaccgg attctgtgcc
     1561 gtaggcaatg gtgatctttt taccactggc tgccggcttg gccaccggat tggcgttctc
     1621 cggcattttg gaaagcacgc cttgcaggaa cccgatcatc cagatgagat cggactgctc
     1681 cagggtagcg atcagttcct gtatctgttt atttttttct tctgatatca tgctctttcc
     1741 tcctgctgct gtgttttttt aatgggttta aaataaagtt catcggatcc gttatcgggc
     1801 ctccaccgga attgctcatg caatgcggcc actttgccga tgatgagtat ggtgggggaa
     1861 agatagtttc ggttccccgg ccggttggca tattcgttca gcgggcagct gtatacgcgc
     1921 tgcatcgggg tggtcgcctg ttcgatgacg gcaatatgcc ggtctgattt tataccattc
     1981 cggagaagat tatcggcgag ttcatcggaa aggtcgccgg acatataaaa caccagggtg
     2041 tcctccgtgc cggccagctc tttccagtat tccggttcga tcaacccggt cttatgaagc
     2101 gtaagcaggc gcacggcatt ggaatagttc cgggcggtga gcggaatgcc ggcataggct
     2161 gccgcaccgc atgccgcggt aatgcccgga acgatctcgt agggaatccg gtacctgttc
     2221 agtgcctgta attcatccag caggttggaa aaaacagaaa cgtctccgcc tttcaggcgc
     2281 accactcttt ttccctgcag cgcatattca accagtaatt catttatgat atgctgcggg
     2341 gtagatcctt ctttaccccg ttgtttaccg gcatggagga ccagcgccga cttgctcaca
     2401 tatttctcca ggatcacttc gctcacaaga cggtcggtga tgaccacttc cgcctcccgc
     2461 aggtgacggg ctgtttttac cgttatcaat tccggatctc ccggtcctgc gccggcaatg
     2521 atcagcttcg gcagattttt attttggttc atcccgctgg attaaaattt aaaatttatt
     2581 cctgtatcat gcaggctcct accgttacat tactggtttc atctatcaat atcagtcccc
     2641 cgttcgcccg cagatcctta tacagatcat aggcgagggg agaggccgtt tttatttttg
     2701 cccgcacgat atcattgagc tcggcttttt caacgccttc ctgtttttca agggagttca
     2761 catcaagctt gtattcgatc tcccggacga cggatttaac ccggcggctg ttgtgctgca
     2821 ggaaatacct gttccccgct ttcagcggtt tgctgtccat ccagcataac agcacttcca
     2881 gttcctggcc cagcagcggg ggaaaatctt ttttcaccaa cacgtcaccg cggctgacat
     2941 cgatatcgtc ttccaggtga aggatcacgc tttgtcctgc caccgcttcc tccacctcct
     3001 gctggtttct ttcgatccgg gcaaggcggg tattgatccc agcgggaaga ataatgatct
     3061 cgtctccttt ccggtaaaca ccgctgataa tttttccggc gtatccgcga tagtcgtgca
     3121 attcctcgct ttgcggccgg atcacatact gcacgaagag gcgggcatgc gtcaggttaa
     3181 tgtccgaaga aatggaagtc gtttccagga tctcgagtaa gggcaatcct tcataccagg
     3241 gcgtgttcac ggaaaggtcc acgatattgt cgccgttcag tgcgctgatc ggcaggtact
     3301 gaacattctt tagtccgagg gaacgggcaa aggaggcata ttcaatcaca atattattgt
     3361 agacatcctg ggaatattcc acgaggtcca ttttattgac cgccaccacc acgtgcggca
     3421 tgttcagcag cgaggcgatg atggaatgcc ggcgggtctg ctccacgatc ccgttgcgcg
     3481 catccaccag gatgatcatc agctgggcat tggacgcacc ggtgaccata ttccgggtgt
     3541 actgggtatg tcccggagca tccgcgataa tgaatttacg tttgggggtg gagaaatatt
     3601 tataggccac gtcgatcgta atgccctgtt ccctttctgc acgcagtccg tcggtaagca
     3661 gggccaggtc gatcgattct tcttccttgt gttttctctg tttttccagc gcttccagct
     3721 gatccgtcag aatgctttta ctgtcgtaca acaaacgccc gataagggtg ctcttgccgt
     3781 catccacact tcctgccgtt ataaatcgta aaagatccat gttatgattg tcggtgttta
     3841 aaaatatccg ttcttctttc tgtcttccat ggccgcttcg gaaaggcggt cgtcgattct
     3901 cgtttccccg cgttcgctgg tgcgggtggc gatgatttca ttgataatat cgtttacggt
     3961 ggacgcactg gattccacgg cggcggtgca ggacatatcc ccgacggtgc ggtaccggac
     4021 ctttttattg accactttgt cgttttcatc cagccggata aaagcggaaa cggcaacgag
     4081 ctgcccttca tattcgatca cgtcccggtg atgggaaaaa taaatggaag gaagggtaat
     4141 gttctcgcgg cggatataat tccagatatc gagttccgtc cagttgctga tggggaatac
     4201 gcgcacgttc tctcctttct ggatctttcc gttatagata ttccagagct ccggcctttg
     4261 tcttttagga tcccactggc cgaactcatc gcggacggaa aaaatccgtt ctttcgcgcg
     4321 ggccttttct tcatcccgcc gggctccgcc gatgcaggca tcgaatccga attcctctat
     4381 ggtat
//
LOCUS       DASYCU010000337         7107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2828688, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000337 DASYCU010000000
VERSION     DASYCU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828688"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VGM24_06825"
     CDS             <1..1409
                     /locus_tag="VGM24_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4155120.1"
                     /translation="NKKEIEGTAIEHWFTADHIKLFKSIELKLASQQAKDWQGELELT
                     SGEKAFFAFVNIVPFLDNNRRFLKISILDLSEIKTAQFELMKAKQKAEVASQAKSRFL
                     SNMSHELRTPLNGIIGTSNLLLQENYLAEQKSHLDILKYSSEHMMMLINEILDFNKIE
                     VGKLQLGQEPVDLKKFLEKITLQFAAQAHAKNIGFKTDIDESLDIEVMTDETRLNQVF
                     SNLLSNAIKFTHFGEIVVVARKIYSTSQKATIQFMVKDTGIGIPANKQVEIFESFTQA
                     DVNTTRKYGGTGLGLAITKKLVSLFQGELQLESEEGRGSTFHFTLKLTISENRKRYIN
                     EDKVKHLCMFPGVKVMVAEDNAINMSIAKRFLSKWGIEVKEAYNGRQAVELFAQNNFD
                     LVLIDLEMPEMDGITALSEIKKINPAVAAIAFTAAVYDDMRADLLRKGFMEFVPKPFR
                     PEDLHSKISILLSAQTSA"
     gene            1536..2549
                     /locus_tag="VGM24_06830"
     CDS             1536..2549
                     /locus_tag="VGM24_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155121.1"
                     /translation="MKAILASRMVLAISIAAGLSLYSCKKESSSNHSSSSSENLSTAG
                     DDQAQVSDESDAISEDANTALNSQAAFSGVDNSSTTISGGTAVNAVETNKLGGGHFSI
                     PGLICDATVTYDTSNGQRIITIVYDGTNCRGNRTRTGTVVITLPYGKYWKDAGTSATV
                     DMQQLKITRIRDGKSIVIDGSKTFTNVSGGLLKDLATLGTITHTIKSDGITVTFNNGK
                     QRVWQISKQRVFTYDNGIVITTTGTHSDGTDTNIAIWGTNRFGDSFESLISEPKVFRQ
                     DCDFRLTGGQNTVKISSGTSVITYGLDANGDPTGCPGTGTYYLKLVWTGVNGQTYTVI
                     MPY"
     gene            complement(2654..3772)
                     /locus_tag="VGM24_06835"
     CDS             complement(2654..3772)
                     /locus_tag="VGM24_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155122.1"
                     /translation="MKSRRDFLQKMTASALGLSLLPGLASAGIPILDELHEDDRVLRV
                     AILGLGSYATRVAEAMQSCKKARLVGLISGTPAKIKSWREKYNIPEKNCYNYENFDNI
                     KDNPDIDAVYVITPNGLHHDQVIRVAKAGKHAISEKPMAVNAKEAQEMIDACKENHVR
                     LLVGYRMHFEPHTLEIIRMRKAGEFGKITFFQGLSGFIIGDPNQWRLNKKLSGGGSMM
                     DIGIYSINGSRYMTGEEPIWVTAEETKTDPVKFKEGVDETIQFQLGFPSGAVASCLST
                     YNMNNLDRFFLDGKNGFAELYPATGYGPIQGRTNKGEINQPIVTHQTVQMEEMSDIIF
                     KNKKPVVPIDGEEALKDLKIIDAIYETIRTGKKIDLKA"
     gene            4048..4548
                     /locus_tag="VGM24_06840"
     CDS             4048..4548
                     /locus_tag="VGM24_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155123.1"
                     /translation="MITKTSIIVFFSQSKVIFATVAAIITFTITLYNQFKSPPVTEIS
                     GYVSAEKDAVVPVDAVVKIISPVQSQTETDAKGRFKFKLQNIQSDTFLLVIQNKKTNI
                     ETKQDEYVNASSGKKDIFVLFNSSSDTEKVYAPWGKSTSPAYSKRKPEVLKIIQQLHL
                     PRRRAH"
     gene            4671..>7107
                     /locus_tag="VGM24_06845"
     CDS             4671..>7107
                     /locus_tag="VGM24_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEY4155124.1"
                     /translation="MKWPVNCIRILFTLLVIVLSFTDSYAQAGFQFARLDFTQGLSNN
                     QVNAIYKDAKGFMWFGTMSGLNRYDGYQFKIFRHNLSDTATLNDDYISRICEDPFKKL
                     WIQTREGYNIYDPETEKFNRRPSTYLKSLSLPEAGFSDVIQTKNGFWFLYSGTGLYYF
                     RTGKKPEFLHPVAGDAGSISADPVADIREDSKGYSWLVHQNGLLEKLDNRNNRVIFRT
                     EILKNRNAGRSLAYHLFIDGQDGIWFYSAGNLQGVYYYQPGKGTLQHLSKMNSRGGLN
                     NNLVNGIVEDANGLIWIGTDHGGVNLFNKKEGSVRFVTNSPDDNKSLSQNCITTLYRD
                     VQGGIWVGTYKGGISYYHEDMVRFPWYRRQPATVNSLPYDDVNRFVEDASGNLWIGTN
                     GGGLIYFDRKKNSFRQYLHNPADPNSLSNNVVVSMWLDHKNILWIGTYFGGLDSYDGK
                     HFVHYRHQDAVSGSLADDKVWEIYEDSKENLWIGTLGGGLDKFDRNRKIFVHNKAALP
                     NSIHSDYISALTEDRYNNLWIGTSVGIDVWQNDNRIIHYSTDDKLSNNNIIAILKDDR
                     GNMWVGTREGLNVFNPEKQRFQSFRMEDGLPDNAILTIVEDDSNHLWVTTPNGISRIK
                     VLGSAGSGFRIHCRNYDETDGLQGREFNENAALKTRSGELVLGGANGFNIFNPANIKS
                     SQKNAALVLTDFQVFNKTIHAGDKVNGKRILDKAISETDEVTLKYNQNVFSVEFASLG
                     FNNTDKCRYAYRLVGFDRDWLVTDGNARKATYTNLDHGKYVFMVKASNDDGVWNESAK
                     PLTIIILPPFWETP"
BASE COUNT         1911 a         1673 c         1755 g         1768 t
ORIGIN      
        1 ggaataaaaa agaaatagaa ggaacggcga tagagcattg gttcaccgcc gaccatatca
       61 aattattcaa aagcattgaa ttgaagctgg ccagtcagca ggcgaaggac tggcaaggag
      121 aactggagct gacttcgggt gaaaaggctt ttttcgcttt tgtgaatatc gtgccctttc
      181 tggataataa ccggcgcttc ctcaagatca gtatcctgga tctttcggag atcaagacgg
      241 cgcagtttga attaatgaaa gcgaagcaga aagcggaagt ggcatcacag gccaaatcca
      301 ggttcctttc caatatgagc catgaactgc gcaccccatt gaacggcatc atcggtacat
      361 ccaacttgtt attgcaggag aattatcttg cggaacaaaa atcgcatctc gatatcctga
      421 agtattcttc ggagcacatg atgatgctca ttaacgaaat cctggatttc aataagatcg
      481 aggtgggcaa attacaattg ggccaggagc ccgttgacct taaaaaattc ctggaaaaga
      541 taacccttca gtttgcggcc caggcgcatg cgaagaatat cggttttaaa acagatatcg
      601 acgaaagcct ggatattgaa gtgatgacgg atgaaacgcg cctgaaccag gtattcagca
      661 acctgttgtc caatgcgatc aagttcaccc atttcggaga gatcgtcgtg gttgcccgga
      721 agatctattc aaccagtcag aaagccacta tccagtttat ggtgaaggat accggcatcg
      781 gtattcccgc caataaacag gtggaaatat ttgaaagctt cacgcaggcg gatgtaaaca
      841 ccacgcgcaa gtacggcggt acgggccttg gcctggcgat caccaaaaag ctggtcagcc
      901 ttttccaggg tgaactgcag ctcgaaagcg aagagggcag gggaagcact tttcatttta
      961 cgctcaaact gaccatcagc gagaatcgca agcggtatat caatgaagac aaggtgaagc
     1021 acctgtgtat gtttcccggc gtgaaagtga tggtggcgga agacaatgcc attaatatgt
     1081 ccattgccaa acgcttcctc agcaagtggg gcatcgaagt aaaagaagct tataacggca
     1141 gacaggcagt tgaactgttt gcccagaata attttgacct ggtgctcatt gacctggaaa
     1201 tgccggagat ggatggcata acggcgctca gtgagatcaa aaaaataaat ccggccgtgg
     1261 ccgccatcgc ctttaccgcc gcggtatacg atgatatgcg cgcggacctg ctgcgcaaag
     1321 gatttatgga attcgtaccc aagcccttcc gcccggaaga ccttcatagt aaaatttcaa
     1381 tcctgctttc cgcccagact tcggcctaag gatccgcatt cttccctgtt acaacctgca
     1441 ttttctccgc ctaacgggtc gttaacaaaa aaaatccggt ttaccgttaa accttattta
     1501 acaaaacgga tctgagatga aaatctttgt aaattatgaa agctattctt gccagccgga
     1561 tggtccttgc tataagtatt gccgccggat taagtcttta ttcctgtaaa aaggaaagtt
     1621 cttccaacca ttcttcttct tcctctgaaa atctttctac tgccggcgat gatcaggcgc
     1681 aggtttcgga tgaatcggat gcgatatccg aggatgccaa tacggccctg aacagccagg
     1741 ccgctttttc cggcgttgac aacagctcaa cgactatttc cgggggaacg gcggtaaatg
     1801 ctgtggaaac aaacaagctg ggcggaggtc atttcagcat accgggcctg atctgtgatg
     1861 ctacggtcac ctacgatact tcgaacggcc agcgtatcat aaccatcgtg tatgacggca
     1921 ccaattgccg ggggaaccgg acgcgcaccg gaacggtggt gatcacgctg ccttacggaa
     1981 aatattggaa ggatgcggga accagcgcca ccgtcgacat gcaacagctg aagatcaccc
     2041 gcatacggga tggaaaatca atcgtgatcg acggatccaa aacctttacc aatgtgagcg
     2101 gcgggttgct gaaagacctc gccacgctcg gaacaatcac ccataccatc aagagcgatg
     2161 gcattacggt cactttcaac aacggtaaac aacgggtatg gcagatttcc aaacagcggg
     2221 tatttaccta cgacaacggc attgtgatca ccaccaccgg aacgcattcg gacggaacag
     2281 acacgaatat cgcaatctgg ggaaccaatc gttttggaga ttcattcgag agtctgatca
     2341 gcgagccaaa agtatttcgc caggattgtg atttcaggct gaccggcggg cagaataccg
     2401 taaagatcag cagcggaact tcggtcatca cttatgggct ggatgccaac ggagatccca
     2461 ccggttgccc gggtacagga acttattatt tgaaacttgt ctggaccgga gtgaatggtc
     2521 aaacctacac tgttatcatg ccgtattgat ccgtgctttg gtttttataa aaagacccat
     2581 ctctgtgttt tgagatgggt tcttttctga gtttaggtaa acactgtacg ggctggcaaa
     2641 aattttttct gttctatgct ttcagatcta tttttttacc tgtccggatg gtctcgtaga
     2701 tcgcgtcgat gattttcagg tctttcaatg cttcttcccc gtctatggga acaaccggtt
     2761 ttttgttttt aaaaataata tccgacatct cctccatctg aacagtctga tgggtaacga
     2821 tcggctggtt gatctccccc ttgttggtcc ggccctgaat gggcccataa ccggttgccg
     2881 gatataattc tgcaaacccg ttttttccgt ccaggaaaaa gcggtccagg ttattcatat
     2941 tatacgtgga caagcaggag gctacggccc cgctgggaaa gccgagttga aactggatgg
     3001 tttcgtcgac gccctctttg aatttcaccg gatccgtttt ggtttcctcc gcggtcaccc
     3061 agatgggttc ttctccggtc atataacggg aaccattgat cgaatagatg ccgatatcca
     3121 tcatggagcc gccgccggat aatttcttgt tcaaccgcca ttgattcgga tcgccgatga
     3181 tgaatccgct caatccctgg aaaaacgtta tttttccaaa ctcgccggct ttccgcatcc
     3241 ggatgatttc cagggtatgc ggttcaaagt gcatacggta tccgacgagt aaacggacat
     3301 ggttctcttt acaggcatcg atcatttcct gagcctcttt tgcattcacc gccatgggtt
     3361 tctcgcttat ggcgtgcttg ccggctttcg ccacgcggat cacctggtcg tgatgaagtc
     3421 cgttcggcgt gatgacatat accgcatcga tatccgggtt gtcttttata ttgtcgaaat
     3481 tttcgtagtt gtaacagttt ttctccggaa tattatattt ctcccgccag gatttaattt
     3541 ttgccggcgt tccgctgatc aagccgacca gcctggcttt tttgcaggac tgcatggcct
     3601 cggccacgcg cgttgcataa ctgcccagtc ccaggatggc cacgcgcaac acccggtcgt
     3661 cctcgtgcag ctcgtctaaa atcgggatac cggccgaagc aagcccgggc aaaagggaaa
     3721 gtccaagtgc cgaagcggtc atcttttgca aaaaatcgcg gcgtgatttc atctgagtac
     3781 tatttgggtt attatctgtt gatccctctt caaattaaaa ctttcaaatg aaacagcaat
     3841 acaaaatcgg aaagggtggt aaggtatcgg atcttctcct gcggatccgg cccgcagaaa
     3901 agagggcaaa gcggaggagg aattgaagcc tctctcctaa atcattgatc ctgatagatt
     3961 aatcagttat aatttattcc ccgggaaaaa tacaggtttt tataaaccgg agaaatatgc
     4021 tttttcagta ttttagtcct cccgaatatg atcaccaaaa cgtcaataat tgtttttttc
     4081 agccagagca aggtcatttt tgcgaccgtt gccgcgatca tcacctttac gattacactc
     4141 tacaatcaat ttaagtcgcc gcccgtgacg gaaatatccg gatacgtatc tgctgaaaaa
     4201 gatgcggtgg tgcccgtgga tgccgtcgtc aaaattataa gcccggttca gagccagacg
     4261 gaaaccgatg ccaagggcag gtttaaattc aagcttcaga atatccagag tgataccttt
     4321 ctgctggtca tccagaataa aaaaactaat atcgaaacca agcaggatga atatgtgaat
     4381 gcttccagcg gaaaaaaaga cattttcgtt cttttcaatt caagcagtga taccgaaaag
     4441 gtatacgccc cctgggggaa aagcacttct ccggcttatt cgaaaagaaa gccggaagta
     4501 ttgaagataa tccagcagct tcaccttccc agaagaaggg cgcactgaga tttaatcctt
     4561 tgaattccag aaatcccgaa attgaattat ttaattggca ggcgggccgg aaattatcct
     4621 aacttccctc ggctgactgc tgcaagcgga tgcttgtgaa atgatagttt atgaaatggc
     4681 cggttaactg cattcgaata ttattcaccc ttctggtaat tgttttgtct tttacggatt
     4741 cttatgccca ggccggcttt cagttcgcgc gcctggattt cacgcaggga ttgtcgaaca
     4801 accaggtgaa cgccatttac aaagatgcaa aaggctttat gtggtttggt accatgtccg
     4861 gcctcaaccg ctacgatggc taccagttta aaatattccg gcataacctc tcggatacgg
     4921 ctactctcaa tgatgattat atttcccgga tctgcgagga tccctttaaa aaattgtgga
     4981 ttcaaacccg ggaaggctat aatatttatg atccggaaac ggagaagttt aaccgccggc
     5041 cctcgaccta cttaaaaagc ctgtctcttc cggaggccgg gttctccgat gtgatacaaa
     5101 caaaaaatgg tttctggttc ctttattcgg gcaccggcct ttattatttc aggaccggca
     5161 aaaagccgga attccttcat ccggttgccg gagatgcggg cagcatttca gctgatccgg
     5221 tagcggatat ccgggaggat tccaaggggt attcctggct ggttcaccag aacggcctgc
     5281 tggaaaagct ggacaataga aataatagag tcattttcag aacggaaatc ctgaagaaca
     5341 ggaatgccgg caggtctctt gcctaccatc tgtttataga cgggcaggac gggatctggt
     5401 tttattcggc gggcaattta cagggggttt attattatca gcccggtaaa ggcaccctgc
     5461 agcatctgtc gaaaatgaat tcccgcggcg gcctgaataa taacctggta aacggcattg
     5521 ttgaggacgc caacggattg atctggatcg gcacggacca tggcggggtg aatctcttta
     5581 ataaaaaaga aggttcggtc cggtttgtca ccaattcccc cgacgacaat aaaagccttt
     5641 ctcaaaattg cataacgacg ctctaccgcg acgttcaggg aggtatttgg gtcggaacct
     5701 ataagggggg tatcagttat tatcatgagg acatggtccg ctttccctgg tacaggcggc
     5761 agcctgccac cgtcaatagt ttaccctacg acgatgtaaa ccgttttgtg gaagacgcgt
     5821 ccggtaatct ctggataggc accaatggcg gagggttaat ttattttgac cggaagaaaa
     5881 acagcttccg gcagtatctg cataaccccg ctgatccgaa cagtctgagc aataacgtag
     5941 tggtgagcat gtggctggat cacaaaaata tattgtggat cggaacctat ttcggcggac
     6001 tggatagtta cgacgggaaa cattttgttc attaccggca ccaggatgcc gtcagcggca
     6061 gcctggccga tgataaagta tgggaaatat atgaggattc gaaagagaat ctctggatcg
     6121 gcacgctggg gggcggactg gataaattcg accggaaccg gaagatcttc gttcataaca
     6181 aggcagctct tccgaactcc attcattcgg actatatatc tgcgcttacg gaagaccggt
     6241 ataataattt atggatcggc acttcggtcg ggatagacgt atggcagaac gataaccgga
     6301 tcatacacta ttccaccgac gataaattaa gcaacaacaa tatcatcgcc atcctcaagg
     6361 atgaccgggg caatatgtgg gtcggaaccc gcgaagggtt gaacgtattc aatcccgaaa
     6421 agcaacgttt tcaatcgttc aggatggagg atggattgcc ggacaatgcg atcctgacca
     6481 tcgtggaaga cgattcaaat catttgtggg tgaccacgcc caacggtatt tcccggataa
     6541 aggtcctggg ctcggcaggc agcggtttcc ggatccattg ccggaactat gatgaaacgg
     6601 atggactgca gggccgtgaa ttcaatgaaa atgcggccct gaaaacaaga tccggcgagc
     6661 tggtgctcgg cggggcgaac gggttcaata tttttaatcc ggccaacata aaaagcagtc
     6721 agaaaaacgc cgccctggtg ctgaccgatt tccaggtctt caataaaacc attcatgccg
     6781 gggataaggt gaacggcaag cggatcctgg acaaagcgat ctccgaaacg gacgaagtaa
     6841 ccttaaaata taaccagaat gtattctccg ttgaatttgc ctccctgggt ttcaacaata
     6901 cggataaatg caggtacgcc tacagactgg tgggatttga cagggactgg ctggtgacgg
     6961 acggcaatgc gcgcaaggcc acgtatacga acctggatca cggcaaatat gtttttatgg
     7021 tgaaggcatc caacgacgat ggggtatgga atgagtcggc gaagccctta accattataa
     7081 tcctgcctcc cttttgggaa acgcccc
//
LOCUS       DASYCU010000338         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5148840, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000338 DASYCU010000000
VERSION     DASYCU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5148840"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VGM24_06850"
     CDS             complement(<1..269)
                     /locus_tag="VGM24_06850"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY4155125.1"
                     /translation="MEAKFNPDKQHTLNQEISISGTGLHTGINVDMTLRPANPGFGFQ
                     FQRIDLAGRPVIKADCELVTDTSRGTTLEDNGAKVSTVEHILAALV"
     gene            410..1114
                     /locus_tag="VGM24_06855"
     CDS             410..1114
                     /locus_tag="VGM24_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4155126.1"
                     /translation="MEGKKPRILLCEDDPNLGMVLKNYLELNDFDVTLERDGRLGLAA
                     FQREHIDLCLLDVMMPHMDGFTLAEEIRDVNPDVPLFFLSAKTMKEDIIQGYKLGADD
                     YITKPFDSEVLLLKIKAILKRNDDFNKEADNKEFDLGSYHFNPKLRELNHNGQTQTLS
                     PKENELLKMLAEHLNDLLPREAALKKIWGSDTYFNGRSMDVYIAKLRKYLRDDNNIEI
                     VNIHGNGFRLVVPVKA"
     gene            complement(1118..1381)
                     /locus_tag="VGM24_06860"
     CDS             complement(1118..1381)
                     /locus_tag="VGM24_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155127.1"
                     /translation="MSGIFKIISGLIANVLDFAFAGYGLVCDNSFIKINLNHLNFRYT
                     RCRDLWVKCYTTPYLREWYSRVAGKLAELINLEENEKWYLRGR"
     gene            complement(1477..>2220)
                     /locus_tag="VGM24_06865"
     CDS             complement(1477..>2220)
                     /locus_tag="VGM24_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY4155128.1"
                     /translation="LVYVEAQAGIRHVGQHVSGYTESDLVLIGPDIPHLNFDYGLKTE
                     YKQIVVQLKPHFLGNAFDHTPELTAIHNLFERAAFGISFYGKTKISVSEQLKEMQGLS
                     PFDQLVLLLKIFQTLAESGEAITLNEIDTSVKTFLDDKIRMSSVYEYIQAHFDQRPDV
                     NEVASRVHLSTPAFCRYFKKQTKMTFTDFVNQYRISQAKTLLLQGKTVSETGYAVGYE
                     SASHFNKLFKKLTGGNPSQFRKTYILAAV"
BASE COUNT          609 a          516 c          531 g          564 t
ORIGIN      
        1 accagggcag ccaggatatg ctccaccgtg ctgacctttg ctccgttatc ctccagggtg
       61 gttccccggg aagtatcggt caccagttcg caatcggcct tgatcacggg ccggccggcc
      121 aggtcaatcc gttgaaactg aaagccaaaa cccgggtttg ccgggcgcag ggtcatatcc
      181 acgttgatcc cggtatgaag accggttcct gaaatactga tttcctgatt taatgtatgt
      241 tgtttatccg gattgaattt tgcctccatc agattgattg taacccgcaa agatacagtt
      301 tggatggcat tccggaccca ccgtccccga aggagtatta acaaaaaatt accggccgaa
      361 catccttaac cggaaatgaa ttaattttat ttgtaattaa actgatataa tggaaggaaa
      421 aaagcccagg atcttattgt gcgaagatga ccccaacctc gggatggtgt taaagaatta
      481 tctggaacta aatgattttg atgtgacgct ggaacgcgac ggcaggctcg gactggccgc
      541 atttcagcgg gaacatatag acctctgcct gctggatgtg atgatgccgc atatggacgg
      601 cttcactctt gccgaggaga tccgggacgt gaatcccgat gtgccgcttt tttttctgag
      661 cgccaagacc atgaaggaag acattatcca ggggtataaa ctcggcgcgg atgattatat
      721 caccaagccc ttcgacagcg aagtgttatt gctcaaaatc aaagccatct taaagcgtaa
      781 tgacgacttc aacaaagagg cggacaataa ggaatttgac ctgggttcct atcacttcaa
      841 tccgaaattg cgggaactga atcataacgg tcagacccaa acgctttcac ccaaggaaaa
      901 tgagttgctg aaaatgctcg ccgagcacct gaacgacttg ttaccgcggg aggcggcact
      961 gaaaaagatc tggggcagcg atacgtattt taacggaaga agtatggacg tatacatcgc
     1021 caagctcaga aaatatctgc gggatgataa caatatcgag atcgtgaaca ttcatggaaa
     1081 cggcttccgg ctggtggttc cggtgaaagc ctgaccatta ccttccccgg agataccatt
     1141 tctcattttc ttccaggttg atcagttcag ccagtttccc ggcaacccgg gaataccatt
     1201 cccggagata gggagtagta taacatttca cccataaatc gcggcaacgg gtataccgga
     1261 aattcaggtg gttaaggtta attttaataa atgaattatc acagaccagc ccgtacccgg
     1321 caaatgcaaa atccaggacg tttgcgataa gacccgaaat tattttgaat atcccactca
     1381 taaccgatta cttaaaaatt tcatgccaaa aagggctgga atcggaaagt aaattgttag
     1441 tagctgattt tcaggagata acccggccat aaacttttaa acagctgcca ggatataggt
     1501 tttcctgaac tgggaaggat tcccgccggt gagtttttta aacagcttgt tgaagtggga
     1561 agcgctttca taacctacgg cataacccgt ttcggaaacg gttttgccct gaagcagcag
     1621 ggttttagcc tggctgatcc ggtactggtt tacgaaatcg gtgaacgtca tttttgtctg
     1681 ctttttaaaa taccggcaaa aagcaggggt actcaggtgt accctggaag cgacttcatt
     1741 tacatccgga cgctgatcaa aatgcgcctg tatgtattca tacaccgacg acattcttat
     1801 tttatcatcc agaaatgtct ttacgctggt atctatctca tttaaagtga tggcctcgcc
     1861 ggattcagcc agcgtctgga atatcttcag cagcaaaacc agctgatcaa aaggagaaag
     1921 gccctgcatc tctttcaact gctccgaaac cgagatcttt gttttaccgt aaaagctgat
     1981 gccgaatgcc gctctttcaa ataagttgtg gatggccgtt agttccgggg tatggtcaaa
     2041 ggcgtttccc aggaaatgcg gtttcagctg gacgacgatc tgtttgtatt ctgtctttag
     2101 cccgtaatcg aaattcaggt gcggtatatc cgggccgatc agaacaagat cgctttcggt
     2161 ataaccggaa acgtgctggc caacatgccg gatgccggcc tgcgcctcaa catataccag
//
LOCUS       DASYCU010000339         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5148861, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000339 DASYCU010000000
VERSION     DASYCU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5148861"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGM24_06870"
     CDS             complement(<1..383)
                     /locus_tag="VGM24_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155129.1"
                     /translation="MHTKPNISFLAFALMAAGLFAFRKPDNGFRNRSFQTALLSGTPD
                     KLDTGGRAVNLDSAQKCINRFEAVMKQHGFSDVAGEKVNIHIKRTSLITTGEVFGGKG
                     LLDWLTQTEAQYTAAHRTLMIKIQLG"
     gene            757..1107
                     /locus_tag="VGM24_06875"
     CDS             757..1107
                     /locus_tag="VGM24_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155130.1"
                     /translation="MDTTTSLSFPVFLQEADTNLIQPFSMNFSETLGSFGVGILLIAF
                     ILNLLKIIRTDSVIYGLLNLAGAGIACYASYLIPYFPFVILEGVWTVLSLVALVKTIS
                     RIPQSANRPPEGAD"
     gene            complement(1123..1995)
                     /locus_tag="VGM24_06880"
     CDS             complement(1123..1995)
                     /locus_tag="VGM24_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4155131.1"
                     /translation="MNLSIASLNSGSNGNCYYIGNEEEAIFIDAGLSCAETEIRMKRL
                     GLNMERIKGIFISHEHSDHILGLSAIVQKYKVPVFINSGTLREVRGKRLRAFVQLTES
                     HKPVSVGGLRITPFPKKHDARDPHSFIVSYRQTTVGIFTDIGQPCEQVVHYFGQCHAA
                     FLEANYDEDLLQNGKYPYFLKNRIRGGNGHLSNHQALDLFKTHRSAFLSHLLLSHLSR
                     ENNSPELVRSLFDSFKTDTRVIIASRYEESLVYSISHSAVAETDPDPLLPAYGFSPKK
                     RRNVNTVLEQLRLF"
     gene            2030..2689
                     /locus_tag="VGM24_06885"
     CDS             2030..2689
                     /locus_tag="VGM24_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155132.1"
                     /translation="MEEPFLTFRKFNDPAMASEMAGRLREHGIPVEIEDSGKLFDPSF
                     AHNYLDQDIRLKLRARDFGEADGIMQSFYESQVHRVGEDYYLYQFSNSELLEIVRNPD
                     EWGYLDYMLAQKILSEHGISIPSRELKRFQNEKIKLLSEPEHADKSWTYMGYLLALLF
                     PPLGMIIGASMGFMKKTLPDGQRRFAYSESDRKQGKQILIISEVCLVPWVWLWLRGWK
                     I"
     gene            2757..3395
                     /locus_tag="VGM24_06890"
     CDS             2757..3395
                     /locus_tag="VGM24_06890"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEY4155133.1"
                     /translation="MDAQAKGRRPFLLAGTFSGWIAIILQFYLILIHRDASVRETIIR
                     YFSFFTILTNILFTVCFTFLLISPDSGPGKFFSRPATLAAIAVYMAVVGIIYNGVLRF
                     LWNPRGFQWFADELLHVVNPVCFLLFWIFYVPKTGLQWKQAFIWMLYPVIYISLILFR
                     GSFSGFYPYPFSNVRKLGYHRVFLNSTRTILVFLVIALLFVLIGKRQEKLKK"
     gene            complement(3419..4504)
                     /locus_tag="VGM24_06895"
     CDS             complement(3419..4504)
                     /locus_tag="VGM24_06895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY4155134.1"
                     /translation="MILSPILSAYGLREEQLEIIPLGSGLINHTWIIRAPDRALILQR
                     INDTVFKQPEDIEENIRLIGSFLKQHHPDYLFTAPVKTADGREMIRDPGQGYFRMFPF
                     VESSHTVTVVNNTVQAWEAANQFGLFTKKLAGFPVNALKYPLPDFHNLSLRYEQFLQA
                     LENGNKQRIRENRVWIEKARQQESIVAGYERIKNHPGFRIRVIHHDTKISNVLFGPTD
                     KGLCVIDLDTVMPGYFISDVGDMMRTYLSPVSEEEKDFGRLEVREDFFRAIVNGYLHE
                     MKDELTEEEKTAFVYSGKFMIYMQALRFLTDYLNNDIYYGAKYEGHNLVRACNQLTLL
                     ERLIEKESSLIGILKEENGPRVLGSFY"
BASE COUNT         1129 a         1116 c         1112 g         1280 t
ORIGIN      
        1 cccagctgga tctttatcat gagggttctg tgtgccgccg tatattgcgc ttctgtctgg
       61 gtaagccagt ccagcaggcc cttcccgccg aatacttccc ccgtggtgat caggctggtc
      121 cgcttgatat ggatattcac tttttcaccg gccacatccg aaaagccgtg ctgtttcatc
      181 acggcctcaa accggttaat gcatttttgc gccgaatcca ggttgacggc cctgccgccg
      241 gtatccagct tgtccggtgt tccggaaagc agagcggttt gaaaggaccg gttccggaaa
      301 ccattgtccg gtttacgaaa agcaaataag ccggctgcca tcagggcgaa cgcaagaaag
      361 gaaatattag gtttggtatg catatttatc attttaaagg ttatttgtgc ttcaaaatac
      421 ggagaatagc cacatgaaat gcgggcaggc ccagaaacgg gcatttcccc cctccggtac
      481 cgggaaaaat accggggtca gaatccagct ttctcccctt gtttgggcct ggccttccgg
      541 cgtaagtttg ttctcaggcc ccaaccttca aaaccattga ccattacctt atccgggacg
      601 ggctcggaat acagattttg acacttatta attttctcgt ggactagcaa tcgttaactg
      661 cctccctgtt cccagggagg cttttttaga cagaaacaga acaaataatc ataaacggca
      721 ggcaggattt cgtactttgc gcattgaagt ttttcaatgg atacgactac cagccttagt
      781 ttcccggttt tcctgcagga agcggacacg aacctgatcc aaccattttc catgaatttt
      841 tcagaaacct taggaagttt tggggtgggc attcttttaa ttgcctttat actcaacctc
      901 ctgaaaataa tccggacgga cagcgtaata tatggcttgc tgaatctggc cggcgccggc
      961 atagcctgtt atgccagtta cctgatccct tacttccctt tcgtgatcct ggaaggcgtc
     1021 tggacggtct tatcgcttgt cgcgctggtt aaaacgattt ccaggatccc gcaaagcgca
     1081 aaccggcccc cggaaggagc ggattagccc tatacctgac cgttagaata agcgcaattg
     1141 ctccagaaca gtatttacgt tcctcctttt cttaggggag aatccgtacg cgggcagcag
     1201 cggatcggga tccgtttccg caacggccga atgggagatg gaatagacca ggctttcctc
     1261 gtacctggat gcgatgatca cccgggtatc cgttttaaag gaatcgaata acgagcggac
     1321 cagttcgggg gaattgtttt ccctggaaag atgcgataac agcaggtggc taaggaaggc
     1381 agaccggtgt gttttgaaaa ggtccagcgc ctggtggttg gacaggtggc cattgccacc
     1441 cctgatcctg tttttcagga aataaggata tttcccattc tgcaacaggt cctcatcata
     1501 attcgcttcc aggaaagcgg catggcattg tccgaaataa tggaccacct gttcgcaggg
     1561 ctggccgata tcggtaaaaa taccgacggt tgtctgccgg tagctcacga taaaactatg
     1621 cggatcgcgg gcgtcatgtt tttttggaaa cggcgtgatc cggaggccgc cgacactcac
     1681 cggcttatgg ctttccgtca attggacgaa tgccctcagt ctcttgcctc tcacttccct
     1741 cagggtaccg gaattgataa acaccggaac cttatatttc tgaacgatgg cagacaggcc
     1801 gaggatatga tcgctgtgtt cgtgggaaat aaaaattccc tttatccttt ccatattcaa
     1861 tcccaggcgc ttcatccgga tctccgtttc ggcgcaggat agtccggcgt ctatgaaaat
     1921 ggcttcttct tcgttgccga tataataaca gtttccgttg ctaccggaat ttaatgaggc
     1981 gatggaaaga ttcataactg attgcgaaat taatggtaag tttattgcca tggaagaacc
     2041 ttttctcaca ttccggaaat tcaatgatcc ggcaatggct tcggaaatgg ccggcaggct
     2101 gagagaacat ggaattccgg tggaaataga ggacagtgga aaactttttg atccgagctt
     2161 tgcccataat taccttgacc aggatatccg gctgaaactc cgggcccggg atttcgggga
     2221 ggcagacgga attatgcagt ccttttatga aagccaggtg caccgggtgg gggaagacta
     2281 ttatttgtac cagttcagca attccgaact gctggagatt gtccggaatc ccgacgaatg
     2341 gggatatctg gattatatgc ttgcgcagaa gatcctatcg gagcatggca tttccattcc
     2401 ttcccgggag ttgaagcgat ttcagaatga aaaaataaag ttgctttccg aaccggaaca
     2461 cgcggataaa tcctggacct atatgggata cctgcttgcc ctgcttttcc cgccgctggg
     2521 aatgatcatc ggggcctcaa tgggttttat gaaaaaaacc ctgcccgacg gccagcgccg
     2581 ctttgcgtat agcgaatcgg acaggaaaca ggggaaacaa atactgatca tttcggaagt
     2641 ctgtctcgtt ccgtgggtct ggctctggct ccggggatgg aagatttaat tcgagggata
     2701 cgtacatatt taaacggaat tttttaaata taacggatga attaaatgct tatgaaatgg
     2761 atgctcaggc aaaaggcagg cggccattct tattagccgg gactttttca ggatggatag
     2821 ccatcattct tcagttttat cttatcctga ttcaccggga tgcttccgtc cgggaaacca
     2881 ttatccggta tttcagcttt ttcaccatcc ttaccaatat cctgtttacg gtttgcttta
     2941 ctttccttct tataagtccg gactccggcc ccgggaaatt cttttccagg cccgctacgc
     3001 tggccgccat tgccgtgtac atggcggtcg ttggaatcat ttataacggg gttctccggt
     3061 ttctctggaa cccccgggga tttcagtggt ttgcagatga attgctgcac gtggtgaacc
     3121 cggtctgttt ccttctattc tggattttct acgtgccaaa aaccggtttg cagtggaaac
     3181 aggcatttat ctggatgctg tacccggtga tttatatctc gctcatcctg ttccgcggat
     3241 ctttttccgg cttttatcct tatccgttca gcaatgtccg caagctcgga tatcatcggg
     3301 tattcctgaa tagtacaaga accatcctgg ttttcctggt gatcgcgctg ttgtttgtcc
     3361 tgatcgggaa aagacaggaa aaattgaaaa aatgaaagaa ttatattgaa agagagcatc
     3421 aataaaaaga ccccaatacc cggggtccgt tctcctcctt caggataccg atcagcgaag
     3481 attctttctc tatcagcctt tccagcaggg tcagctggtt gcatgctctt acgagattat
     3541 gcccttcata tttcgctcca taataaatat cattattgag ataatcggtg aggaagcgga
     3601 gcgcctgcat atagatcatg aatttgccgg agtaaacaaa agcggttttc tcctcttccg
     3661 ttaattcatc cttcatctcg tggagataac cgttcacgat ggccctgaaa aagtcctccc
     3721 gcacctccag cctgccgaag tccttttctt cttcgcttac cggcgaaaga taagtgcgca
     3781 tcatgtctcc cacatcactg atgaaataac cgggcatcac cgtgtccagg tctattacgc
     3841 aaaggccttt gtccgtaggg ccgaacaaca cattactgat cttggtatcg tgatggatga
     3901 cccgtatccg gaacccggga tggttcttta ttctttcgta cccggctacg attgattcct
     3961 gttgcctggc tttttcaatc cagaccctgt tttcccggat gcgctgctta ttcccgtttt
     4021 caagagcctg caggaactgt tcgtagcgaa gggaaagatt atggaaatcg ggaagcggat
     4081 attttaatgc attgaccgga aagccggcca gctttttcgt gaacaatccg aactggttcg
     4141 ctgcttccca ggcctgcacg gtattgttga ccacggtaac ggtatgtgaa ctttccacaa
     4201 aaggaaacat cctgaaataa ccctgaccgg gatcccggat catttcccgg ccatcggctg
     4261 tttttacggg agctgtaaaa agatagtcgg ggtgatgctg cttcaaaaag cttccgatca
     4321 aacggatatt ctcttcaata tcttccggtt gtttaaatac ggtatcgttg atgcgctgca
     4381 ggatcaaagc ccggtccggg gccctgatga tccaggtgtg attgatcagt ccgctcccga
     4441 gaggtattat ctcaagctgt tcttctctta gtccgtaggc agataaaatg ggagacaata
     4501 tcatttcaat ggaatatccg gtaaacttat aaaattatat cagctttcta ttaaataact
     4561 gtggcaatcc ggttttcctt ttattgatta ccttaataca aaataatttt aataattggc
     4621 actaatgaaa acgatct
//
LOCUS       DASYCU010000340         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1959884, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000340 DASYCU010000000
VERSION     DASYCU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959884"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VGM24_06900"
     CDS             <1..527
                     /locus_tag="VGM24_06900"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4155135.1"
                     /translation="LLLLNGAFRYAPNLEALENLVFQINPLLQKEGFPYQILVCGMDI
                     PEKFKNTRFPNLMAIGYVKDIETYLKGCQVFLNPVIRGAGIKTKLVEALGYNLNSVST
                     ENGSVGIDPVLCNGKLLVCPDSNWEEFARLIRQMAEIQIPIPQAFYDHFYWDNIARRA
                     AEFIQTGAAREYGI"
     gene            complement(559..1026)
                     /locus_tag="VGM24_06905"
     CDS             complement(559..1026)
                     /locus_tag="VGM24_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HEY4155136.1"
                     /translation="MAYENNDKRMESVYSKRIRAGKRRTYFFDVRATRSNDYYLTITE
                     SRKKFNEDGYDRHKIFLYKEDFNKFIKALTEAVDYVKTDLMPDFDFDAFNHDQLPENG
                     ENGIHENGETTRSEPYLDPVVQVSAAEIKEESTAPAPAPSSSGSDHLEEVDKW"
     gene            complement(1144..3054)
                     /locus_tag="VGM24_06910"
     CDS             complement(1144..3054)
                     /locus_tag="VGM24_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4155137.1"
                     /translation="MKYLRAVNKYFWKYKWRLLLGLVFVILSNYFRILSPQVTGYVVN
                     EVEKELSSPHVSGTGAPGANPGNAYSGNRRPSANQKSDTANYDILVKKFIRKLNVSHQ
                     SFGKKVALSGITLLVLAVIGGAFLFLMRQTIIVMSRHIEFDQKNEIYRHYQLLDSSFY
                     KAHSTGDLMSRMAEDVSRVRMYTGPAIMYLGNLLATIGFSLYFMIRKDALLTLYVLAP
                     LPILAVTIYLVNTIIHKRSERIQALLSNLTTNAQESYSGIRVIKSFVQENAMIRFFTN
                     NSEEYRKNAINLAKVEAIYFPSMGLIIGLSTLLTIMIGGFYVIRGTHHTDLGTITEFV
                     IYITMLTFPVSAIGWVASMIQRASASQKRLNEFLETVPAIQNPPQPVSQPLQGSISFE
                     RVDFIYPNTGIHAVKNFSLKINKGEKIAIIGKTGSGKTTLAQLLLRFYDPTHGELQID
                     GTNIRTMDLHSLRSQIAYVPQDVFLFSDTVTGNILFGNDKKMEPLARKAAGFASVDQE
                     IGQFSSGFDTLIGERGVTLSGGQKQRISIARALSTDHEIVVFDDCLSAVDARTEKEII
                     SHLYAYLQNKTALIITHRIFSLFDFDRIVVLDQGKIVESGTHNALLAMNGLYARLYEQ
                     QQQTGGEVQTRN"
     gene            complement(3141..>3430)
                     /locus_tag="VGM24_06915"
     CDS             complement(3141..>3430)
                     /locus_tag="VGM24_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEY4155138.1"
                     /translation="TGIFATKTVNPAGNDGWFYGNFSFFWTQIKALLIVVPYSFIVSY
                     GIFKFINFILPLRVTQAEEEEGLDASQHAEKYLQGTLLVSTPEGLAEREAF"
BASE COUNT          840 a          807 c          855 g          928 t
ORIGIN      
        1 cgctcttgtt gttaaacggc gcattccggt acgctcctaa cctggaagcc ctggagaacc
       61 tggttttcca gataaatccg ctcctgcaaa aagagggatt tccctaccag atcctggttt
      121 gcggaatgga tatccctgaa aaattcaaga acacccggtt tcccaacctg atggctatcg
      181 ggtacgtgaa ggatatcgaa acctatttaa agggatgcca ggtatttttg aatccggtga
      241 tccggggagc cggcatcaaa accaaactgg tggaagccct tggatataac ctgaattcgg
      301 tttcgacaga aaatggctcc gtgggcattg atccggttct ttgcaacgga aaactactgg
      361 tttgtccgga cagcaattgg gaagagtttg ccaggttgat ccggcagatg gcagaaatac
      421 agatccccat tccgcaggct ttttacgatc atttttattg ggacaatatt gccagaagag
      481 cggcggagtt cattcaaacc ggagcggccc gggaatacgg gatctaaaaa aaatgcctcc
      541 cggaggaggc tgatgcgttt accatttatc cacttcttcc agatggtctg aaccggagga
      601 agaaggagcc ggagccgggg ccgtcgattc ttcttttatt tctgcggctg aaacctgaac
      661 gacgggatcc aggtatggtt cactacgggt tgtctcgccg ttttcgtgaa tgccgttttc
      721 cccgttttcc ggaagttgat catgattgaa ggcgtcgaaa tcaaaatcgg gcatcaggtc
      781 cgtttttaca taatccaccg cttccgtaag tgccttgata aatttgttga agtcttcctt
      841 gtaaagaaaa atcttatgac ggtcgtatcc gtcctcatta aattttttac gactctctgt
      901 aatggtcagg taataatcgt tgctgcgtgt tgcccgaacg tcaaaaaaat aggttctcct
      961 cttccctgct ctgattcttt tactgtaaac actctccatc cttttgtcat tattctcgta
     1021 agccactttg gttggtttta ggtaaataaa ttatacaagc gcttacaaat ataatattgt
     1081 tccagaactg gcaaaaattt ccccaatata aaactcagga aagggtttat acgcgaaata
     1141 gcctcagttg cgggtttgga cttccccgcc ggtttgctgt tgctgttcat ataacctggc
     1201 atataagcca ttcatagcca gcagtgcgtt gtgggttccg gattcgacta ttttgccctg
     1261 gtccaggaca acgatccggt caaaatcaaa caagctgaag atccggtgcg ttatgatcaa
     1321 tgccgttttg ttctgcaggt aggcataaag gtgggaaatg atctctttct ccgtccgggc
     1381 atccaccgca ctgaggcaat cgtcaaaaac cacaatctca tgatcggtcg acagggcgcg
     1441 ggcaatggag atcctttgtt tttgccctcc gctcaaggta acgccgcgtt ctccgatcag
     1501 ggtatcgaaa ccggaagaga actggccgat ttcctgatcc acgctggcga atccggctgc
     1561 cttccgggcc agcggttcca tcttcttatc atttccgaaa agtatgtttc cggttacagt
     1621 atcactgaaa agaaaaacat cctggggaac ataagcgatc tggctgcgga gactgtggag
     1681 atccatggtt ctgatgttgg ttccatctat ctgtagctcc ccatgggtcg ggtcgtaaaa
     1741 ccggaggagc agctgggcca gcgtggtctt tccgcttccg gtttttccga tgatcgcaat
     1801 tttttcccct ttgttgatct tcaggctgaa attctttacg gcatgaatgc cggtattggg
     1861 ataaataaaa tcgacccgct caaaggaaat gctaccctgt agtggttgag ataccggctg
     1921 gggcggattt tgaatcgccg gaacagtttc gagaaattca tttagtcttt tttgagaggc
     1981 ggatgcccgc tggatcatgc tggcgaccca tcctatggcg ctcaccggga aagtgagcat
     2041 ggtaatatag atcacaaatt cggtgatcgt gcccaggtcg gtatgatggg tgccccggat
     2101 cacgtaaaat ccgccaatca tgatagtcaa tagcgtgctc agtccgatga tcagtcccat
     2161 ggaggggaaa taaatagctt ccactttggc caggttgatg gcattcttcc ggtattcttc
     2221 actattgttt gtgaagaaac ggatcatggc attctcctga acaaaggatt tgatcacgcg
     2281 aatgccgctg tacgattcct gggcattggt ggtgaggttg ctcaggagag cctgtatgcg
     2341 ttcactccgc ttatggatga tcgtattcac caggtagatg gtgacggcca gtattggcag
     2401 cggcgccagt acatacaggg tgagcagtgc atctttgcgg atcatgaaat aaaggctgaa
     2461 gcctatggtg gcgagcaggt tccccaggta catgatggca ggtcccgtat acattctgac
     2521 ccggctcaca tcttccgcca tccggctcat caggtcgcct gtactatgcg ctttgtaaaa
     2581 agaactgtcc agcaactggt agtgccggta aatttcattt ttctgatcga attcaatatg
     2641 gcggctcata acgataatcg tttgccgcat gaggaagaga aatgcaccgc cgatcaccgc
     2701 caataccagg agggtgattc ccgaaagggc gacttttttc ccaaagctct ggtgagaaac
     2761 gttgagtttc cggataaatt tctttaccag gatatcgtag ttggccgtat ccgatttctg
     2821 gtttgccgaa gggcgccggt ttccggaata ggcattcccc ggatttgccc ccggagcccc
     2881 ggttcctgat acgtgcgggc tgcttaactc cttttccact tcattcacca catacccggt
     2941 cacctgtggg gagagtatgc gaaaatagtt ggatagaata acgaaaacca gtccaagcaa
     3001 aagcctccac ttatacttcc agaaatactt gtttactgcc cgtaaatact tcatgccccg
     3061 ttttactgaa cggcaaattt acgtgaatgc aaacatattc ctgaaagcgt ggaggcttgc
     3121 gggtatatgg aattaatggt ttagaatgct tccctttctg ccaggccttc cggtgtggaa
     3181 accagcaaag tgccctgcag gtatttttct gcatgctgac tggcatccag tccctcttct
     3241 tcctctgcct gggtaacgcg caggggaagg atgaagttga tgaatttgaa tattccgtag
     3301 gaaacgataa agctgtacgg aactacaatc agaagggctt tgatctgtgt ccagaaaaaa
     3361 gaaaaattac cgtagaacca tccgtcgttg ccggccggat tcaccgtttt ggttgcgaaa
     3421 atgccggtca
//
LOCUS       DASYCU010000341         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2539312, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000341 DASYCU010000000
VERSION     DASYCU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2539312"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VGM24_06920"
     CDS             <1..270
                     /locus_tag="VGM24_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY4155139.1"
                     /translation="AFFYWLIDVVNIRRWSFFLLVIGANSIAAYVIADGFSSFISDTL
                     YIHLGRHYNQLFGVTYETLVRGGLILLLEWLILYWLFKKKIFIKI"
     gene            325..1746
                     /locus_tag="VGM24_06925"
     CDS             325..1746
                     /locus_tag="VGM24_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155140.1"
                     /translation="MKENRRDFLRLSILAGVGVGSGLGNRLSAQSPEDQSAGPEKKIS
                     GPQHFNMSGFAAPKLNTVRAGFIGLGQRGPTHLNNLSKLDSVQIVALCDLRPEKVSQA
                     QKHLEGSGRTPAVYSGGAEEWKKLCDRKDIDLVYIATPWDLHTPMAVYAMEQGKHVAV
                     EVPAAVSLEECWKLVNTSERTRKHCMMLENCCYDFFELLTLNMARQGYFGEIVHAEGS
                     YIHNLLAENFDKKAYWDMWRLKENTHRNGNLYPTHGLGPVCQIMNINRGDKMDYLVSM
                     SGIDFNMGKKAKELAATDDFFKPYAGMSYRGNMNTSIIRTDMGRTIMIQHDVSSPNVY
                     SRIHKITGTKGTALKYPLPGKISNGGENWLTEPEMKALEEKYQPAIVKRVGEMAKQIG
                     GHGGMDFLLDWRTVDCLRNGLPLDMDVYDAALWSALSPLSEKSVAARSKTIDVPDFTA
                     GSWKTNKPVDILLEEGGNTKVNV"
     gene            complement(2211..2759)
                     /locus_tag="VGM24_06930"
     CDS             complement(2211..2759)
                     /locus_tag="VGM24_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HEY4155141.1"
                     /translation="MAKAKRSIVLADEVIMNKIYMVKGQKVMIDRDLAELYGVETKVL
                     KQAVRRNAKRFPKDFMFEMNTKEFEHWRSQNVTSNQGDRMGLRYAPFCFTEQGVTMLS
                     CILNSERAIAVNIQIIRIFTKLREMIITHKDILLKLEQLEKKVTVHDGDIEMIFSALK
                     QLLNPPQEPRPRIGFRRQNEKE"
     gene            complement(2820..3062)
                     /locus_tag="VGM24_06935"
     CDS             complement(2820..3062)
                     /locus_tag="VGM24_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155142.1"
                     /translation="MRRERAGTKAALTEELKGIHPGIRNALHIRSGVILNWLRQHGKR
                     QMQYMIDHKHIDSTERYQVQDMESLKSALSKHHPFG"
     gene            complement(3146..>3419)
                     /locus_tag="VGM24_06940"
     CDS             complement(3146..>3419)
                     /locus_tag="VGM24_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155143.1"
                     /translation="FNKSLLYRFLIARCDFSTVRLRYADCSPLQNKFVRIANQLPAFR
                     YFLPPAASFNPDQNYSLTQLIKLPGIRLYYEYLKRQDIIEKNPAKH"
BASE COUNT          856 a          849 c          813 g          901 t
ORIGIN      
        1 gccttttttt actggctgat cgatgtggtc aatatccgga gatggtcctt ttttctgctg
       61 gtgatcgggg ccaattcgat cgcggcctat gtgatcgccg acggattttc ttcctttatc
      121 tccgatacgc tttatattca cctgggccgc cattataacc agctattcgg cgtcacatat
      181 gagaccctgg tcaggggcgg gctgatctta ttgctggaat ggcttatctt atactggctg
      241 tttaagaaaa aaatatttat caaaatatag agatccggcc gccggatcct ttccgtcaat
      301 ccttcaccga tcaattaaaa tattatgaaa gaaaaccgca gggatttttt aagactgtcc
      361 atactggccg gggtaggcgt cggcagcgga ctcggtaacc ggctatccgc gcagtccccg
      421 gaagatcagt ccgccgggcc ggagaaaaaa atatccggtc cgcagcattt taatatgtcc
      481 ggcttcgcgg cgcccaagct gaatacggtc cgtgccgggt tcatcggcct gggccagcgg
      541 gggcccaccc acctgaacaa cctgagtaaa ctggattccg tccagatcgt cgcgctctgc
      601 gacctgcggc cggaaaaagt ttcccaggcc cagaaacacc tggaaggctc cgggcgtaca
      661 ccggctgttt attccggcgg tgcggaagaa tggaaaaaac tctgcgaccg caaggacatc
      721 gacctggtct atatcgccac gccctgggac ctgcatacgc ccatggctgt ttacgccatg
      781 gaacagggca agcatgttgc ggtggaagtg cctgcagccg tcagcctgga agaatgctgg
      841 aaactggtta atacctccga gcgcacgcgc aagcattgta tgatgcttga aaactgctgc
      901 tatgatttct tcgagctgct cacgctgaat atggcgcggc agggatattt tggggagatc
      961 gtccatgcgg aaggctccta tattcacaac ctgctggcgg agaactttga caaaaaggcc
     1021 tactgggata tgtggcggtt aaaggagaac acccaccgga acggcaatct ctatcccacg
     1081 catggcctcg ggccggtctg ccagatcatg aatatcaacc ggggggataa aatggattac
     1141 ctcgtctcca tgtccggcat tgattttaat atgggaaaaa aggcaaagga gctggcggcg
     1201 accgacgatt ttttcaaacc ctatgccggt atgtcctacc ggggaaatat gaataccagc
     1261 atcatccgta cggatatggg ccgcacgatc atgatccagc acgatgtatc ctctcccaat
     1321 gtatattccc ggatccacaa gatcaccgga accaaaggca cggccctgaa gtacccgctg
     1381 ccgggaaaaa tttccaacgg gggagaaaac tggctgacgg aaccggaaat gaaagcgctg
     1441 gaggaaaaat atcagccggc gatcgtaaaa agagtgggtg aaatggcaaa acagatcggc
     1501 ggtcacgggg gcatggattt cctgctggac tggcgcacgg tggattgtct ccgcaacggc
     1561 ctgcccctcg atatggacgt gtatgatgcc gccctctgga gcgctctttc tccgctcagc
     1621 gaaaaatcgg ttgccgcccg ttccaaaacg atcgatgtcc cggacttcac cgccggttca
     1681 tggaaaacga acaagcccgt ggatatcctg ctcgaagaag gaggaaatac caaggtgaac
     1741 gtgtgaaaat cggaactttg attgacaata atgccaatat gacagagcga tatctggtct
     1801 ggtttcaaag cggcatcaaa gcggtttcaa agcggtatta aggcgatgga taaaaacagg
     1861 ccgggagccg gcttatttcc atctccactc tgccagattt tcatatttaa tcagtctctt
     1921 ccggacccaa tgctttcttc attgcgaccg gcggggaaat cctttcgttc acttttccac
     1981 tgtaaagctg gtgaaaaaaa ttgcagatac ccctggaagg gtcggcggat tagctggccg
     2041 gcggcggatc acataaatta tttttcctcg ctaccgccgt tatccgcgac gctaaaacaa
     2101 taaaagcata tgcgcgtaac agaccctatt taacctgaca attaataagg gtcggtattt
     2161 attaaatctt ccaatctgtg gatcatggaa gcctgggatc ctggttatgg ttattctttt
     2221 tcattctgcc ttctaaagcc gattcttggc cgcggttctt gcggcggatt caataattgc
     2281 ttcaaagcag aaaaaatcat ctctatatct ccatcatgca ccgttacttt cttttccagc
     2341 tgttccagct tcagcaaaat atccttatgt gttattatca tttcccgcag cttcgtaaaa
     2401 atcctgatga tctgtatgtt taccgcaatg gccctttcgc tatttaaaat acaagacaac
     2461 atggttactc cctgttcagt aaaacagaat ggtgcataac gtaaacccat tctgtcgccc
     2521 tgattggagg tcacattttg tgacctccaa tgctcaaact ctttagtatt catctcgaac
     2581 ataaagtctt tcgggaaccg cttcgcattc cttcttacgg cctgtttcaa tactttggtt
     2641 tcaactccgt ataattccgc caggtcccgg tctatcatca ctttttgacc tttaaccatg
     2701 taaatcttat tcatgattac ttcatccgcc aatactattg atctttttgc ttttgccatt
     2761 tttacagttt gaagtttgca aagatattaa aacaggttac ccgcaaaatg cggataacct
     2821 cacccaaacg gatgatgttt gctgagcgcg ctcttaaggc tttccatatc ctgtacctgg
     2881 taacgctccg tactgtcaat atgcttgtga tcgatcatat actgcatctg ccgtttgccg
     2941 tgttgtctta accagttcag gattacaccg ctcctgatat gcagggcatt cctgatcccg
     3001 ggatggatgc ctttgagctc ttcggtcagc gcagccttag ttcccgctct ctctctgcgc
     3061 aaggttttat agatgtgata aagatgctcc agttcatcat atcttaaagg ctcctcggtt
     3121 accgtctttg gcctgccttt tattctcagt gttttgcagg attcttttca atgatatcct
     3181 gcctcttcaa gtattcatag tagagcctga tgcccggaag cttaatgagc tgggtcaaag
     3241 aataattttg atcaggatta aaagaagcgg cgggagggag gaaataccta aatgccggta
     3301 attgattggc gatgcgcacg aatttatttt gcagcggcga acagtctgcg tatcgtaacc
     3361 ggacagttga gaagtcgcaa cgcgcaatta aaaacctgta caacagggat ttattaaac
//
LOCUS       DASYCU010000342         4483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2393654, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000342 DASYCU010000000
VERSION     DASYCU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393654"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGM24_06945"
     CDS             <1..303
                     /locus_tag="VGM24_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY4155144.1"
                     /translation="RNNKLRPEDIQDCTFTLTNVGIFGSLMGTPIINQPQVAILAVGA
                     IKKRPVVIETEQGDAIGIRHMMYLSMSYDHRIIDGSLGAGFLSAVAKELESFDGEH"
     gene            386..1105
                     /locus_tag="VGM24_06950"
     CDS             386..1105
                     /locus_tag="VGM24_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155145.1"
                     /translation="MKTLSETWFADGYIDFELKKYTLLAYLQEINHHFRENKLYPQLA
                     EVIFHYNNLVAFRENKKYLQEQFPKKLSGLQIEKLQVLYEQMIQDDELMQELEAIIDY
                     SAEHMKTAISNGTEIYEFVEGKFTIQPVGIMPLNSKEGYFLLSEGSSHSTRVYSYHIS
                     FFEKHDEKYRGMRTEYIDSWQRSICNTYEQIKSELLSRNSGFPHPAVYSIETSLRFPV
                     DETLLPVAKRSLVRYISDKAA"
     gene            complement(1081..1980)
                     /locus_tag="VGM24_06955"
     CDS             complement(1081..1980)
                     /locus_tag="VGM24_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4155146.1"
                     /translation="MKKAFIRLHIAVFLAGFTGVLGRLITLNEALLVWYRLLITCAAL
                     WIMSAFMKNRRPPSVSQIARASGIGCILVLHWVSFYAGIKYSTVSTALVCFSAIGFFT
                     AILEPLLLRKSFDVVEVLLGLLVIAGISIVFHFDPRYKTGIIISLVSAFFASVFPVLN
                     RGLLQKMEVEPATRYQLSGGFIFLSLLLPLYLHLFPAARLFPSLPDWLWLLFLALLCT
                     VLAYTLSMSALQRIPAFTVNLTYNLEPIYGIALAFLIYREDKSVSRGFYYGLALIITA
                     IVLQMIRLRRISGKAPQAALSEI"
     gene            complement(1999..2790)
                     /locus_tag="VGM24_06960"
     CDS             complement(1999..2790)
                     /locus_tag="VGM24_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEY4155147.1"
                     /translation="MEKIVIIPTYNEKENVSAIVEAVFDLNEGFHILIIDDSSPDGTA
                     QIVSGLQQKFPGRLFMEERKGKQGLGTAYIHGFKWAVEREYNYIFEMDADFSHNPKDL
                     RRLYEACKYQGADLAIGSRYVQGGGNVNWPANRVILSKGASIYTRLITWMPIRDPTAG
                     FVCYSRDVLQTINLDEILFTGYAFQIEMKFAAWKLGFKIKEVPIIFEDRKLGVSKMNK
                     GIVKEGILGVLNLRWQALFKSYRKRVKNTDSAVNQLPRNMAVEGK"
     gene            complement(2852..3631)
                     /locus_tag="VGM24_06965"
     CDS             complement(2852..3631)
                     /locus_tag="VGM24_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY4155148.1"
                     /translation="MLQLTKPLAVIDLETTGTNLAADRIVEIAIVKLMPDGTRSVKHK
                     LLNPEMPIPKQASDIHGITDEKIKDAPSFRQTANELKQFLDFSDLAGYNSNRFDIPLL
                     VEEFLRVGIDFDMKGRKLVDVQRVFHLMEQRTLTAAYRFYCDKVHDGAHGAQADAEAT
                     LEVLLAQIVRYPQLGTDLDGILKFTGDDQVVDFARRFSMENGVEIFNFGKHKGRAVAD
                     VLKNEPQYYDWMMKGDFPMHTKQKLTEIFNRTMLKKDKTVG"
     gene            complement(3645..>4483)
                     /locus_tag="VGM24_06970"
     CDS             complement(3645..>4483)
                     /locus_tag="VGM24_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY4155149.1"
                     /translation="HFLFPHIAILTGIAWDHINVFPTFDFYLEQFRIFIGMIEPGGLL
                     IYNETDPVLKELVETVAPEIRKTGYGIPEHRVAGGSTSVRLEGISQELQVFGTHNLLN
                     LQAAWLVCRELDVPLKTFLQQIAGFAGASKRLELLGEKGNTRVYRDFAHAPSKVKATI
                     AAMKEQFPDPELIAVLELHTYSSLQAGFMSEYKGVLDPADQALVYYSAHALELKRLPP
                     LDPETVRKGFGTPGLAVISSVQELSAWLRAKSYENSNLLLMSSGNYDGLDIATFVKEI
                     TA"
BASE COUNT         1165 a         1157 c         1100 g         1061 t
ORIGIN      
        1 cggaataata aactgagacc ggaagatatt caggactgca cattcaccct gaccaatgta
       61 ggcatattcg gcagcctgat gggaactccc atcatcaatc agccccaggt ggccattctt
      121 gccgtaggcg ccatcaagaa acggccggtc gtaattgaaa ccgaacaggg cgatgccatc
      181 ggcatccgtc acatgatgta tctgtccatg agctatgatc accggatcat tgacggcagc
      241 ctgggcgcgg gctttctgtc tgccgtagcg aaagagctgg aatcctttga cggagaacac
      301 tgaatccata caaaacaaat ttccaatccc ggcgtttcag ttaagggaag ttttgtaaat
      361 tcaataactc actaaagcca aaacgatgaa aaccctttcc gaaacctggt ttgctgatgg
      421 ttatattgat ttcgaactga agaaatacac cctgctggcc tacctgcagg agatcaacca
      481 ccatttccgg gaaaacaaac tctatcccca gctcgcagaa gtcatttttc attataataa
      541 cctcgtggcc ttccgcgaga ataaaaaata cttacaggaa cagttcccta aaaaactgtc
      601 cggtctgcag atcgaaaaat tgcaggtact ctacgaacag atgatccagg acgatgagct
      661 gatgcaggaa ctggaagcga tcatcgacta ttcggcggag cacatgaaaa cggccatcag
      721 caacggtacc gagatctatg aattcgtgga aggaaaattc accatccagc ccgtgggcat
      781 catgccgctc aacagcaagg agggatattt tttattgagt gaagggtcca gccattccac
      841 gcgcgtatat agttaccaca tcagcttctt tgagaaacac gatgaaaaat accggggcat
      901 gcgcaccgaa tatatcgaca gctggcagcg cagcatctgc aatacttacg aacagattaa
      961 atcggaactg ctcagccgga attccgggtt cccgcatccg gccgtatatt ccatcgaaac
     1021 cagcctgagg tttccggtgg atgaaacgct tttacccgtg gccaagagaa gcctggtccg
     1081 ttatatttcc gacaaagctg cctgaggcgc cttccccgag atccgccgga gccggatcat
     1141 ctgcaacacg atcgccgtaa tgatcagcgc gagcccgtaa taaaaacccc tgctgaccga
     1201 tttgtcttcc cggtaaataa gaaatgccag cgcgatcccg taaatgggtt ccaggttata
     1261 cgtcagattt acagtaaatg ccggtatccg ctgcagggcg ctcatcgaaa gcgtgtaggc
     1321 cagcacggta cagagcaggg caaggaacaa gagccacagc cagtccggca gggaaggaaa
     1381 caaccgcgcg gcgggaaaca ggtgcaggta taaaggaagc agcaggctca gaaaaataaa
     1441 accgccgctg agctggtagc gggtggcggg ttccacctcc attttttgca gcaggccccg
     1501 gttcaatacc ggaaacacgc tggcaaaaaa tgccgagacc agggaaatga taatgcctgt
     1561 tttataccgg ggatcgaaat gaaatacaat actgatcccg gcaatcacca gtaagcccag
     1621 caggacttcc accacatcaa aagacttgcg gagcaacagg ggttcaagta tcgcggtaaa
     1681 aaatccgatg gcggaaaaac agaccagggc ggtggaaacc gtgctgtatt ttataccggc
     1741 ataaaagctc acccagtgca gcaccaggat gcacccgatg ccggatgctc ttgctatttg
     1801 cgacacggaa ggcggccggc gatttttcat aaatgccgac atgatccaga gcgcggcgca
     1861 ggtgatcagc agccggtacc agaccaggag cgcttcgttc agggtgatca gtcttcccag
     1921 aacgccggtg aagccggcaa gaaaaacagc aatatgcagg cggatgaatg ctttcttcat
     1981 gaaggaagca aagaaagatt attttccttc caccgccata tttcggggga gttgatttac
     2041 ggcggaatcc gtatttttca cgcgtttgcg ataactttta aagagggctt gccagcggag
     2101 gttcagcacg cccaggatac cttctttaac gatgcccttg ttcattttcg aaacaccgag
     2161 tttacggtct tcaaaaatga tcggaacctc ttttatttta aacccgagtt tccaggcggc
     2221 gaatttcatt tctatctgga atgcataacc ggtgaacagg atctcgtcca ggttaatggt
     2281 ctgcagaaca tcccgggaat aacaaacgaa tccggccgtc gggtcccgga taggcatcca
     2341 ggtgatcagg cgggtataaa tggaagctcc ttttgaaaga atgacgcggt tggccggcca
     2401 gttcacattt ccgcctccct gtacgtagcg cgagccaatg gccaggtcgg cgccctggta
     2461 tttgcaggct tcatacaaac gccggagatc tttggggttg tgggaaaagt cggcgtccat
     2521 ttcaaaaata taattatact cccgctcaac ggcccatttg aacccatgga tataggccgt
     2581 gcccagcccc tgcttcccct ttctttcttc cataaataag cggccgggga atttttgctg
     2641 aagcccgctg acgatctgtg cggtgccatc gggggagctg tcgtcaatga taagaatatg
     2701 aaatccctcg ttcaaatcga ataccgcttc cacaatcgcg gagacatttt ctttctcgtt
     2761 gtacgtcggt atgattacaa tcttttccac cgggaatatt gacctgtaaa attagctctt
     2821 tatgctgtct tcccggacca atttgacaat attaaccaac cgttttgtct ttttttagca
     2881 tagtccggtt aaaaatctcg gtcagctttt gtttggtgtg catgggaaag tcgcccttca
     2941 tcatccagtc gtaatattgg ggctcatttt ttaatacgtc agcaacggcc cttcccttat
     3001 gctttccgaa gttaaatatt tccactccat tttccatgga aaaccttctt gcaaaatcca
     3061 ccacctgatc atccccggta aatttcagaa taccatcgag atctgttccc aattgtggat
     3121 aacggacgat ctgggccagt aatacttcca gggtcgcttc cgcatctgcc tgtgccccat
     3181 gggctccgtc gtgcacttta tcacaataaa aacgataggc cgcggtcagg gtccgttgct
     3241 ccatgaggtg aaatacgcgc tgcacatcca ccagcttccg gcccttcata tcgaagtcaa
     3301 tcccaacccg cagaaattct tccacgagca ggggaatatc gaaccggttg gaattatagc
     3361 cggccaggtc gctgaagtcg agaaactgtt ttaattcgtt ggctgtctgc cggaaactgg
     3421 gggcatcctt tattttttca tcagtaatgc catgaatgtc ggatgcctgt ttgggaatgg
     3481 gcatttccgg attgagcagt ttgtgcttga cggaccgggt gccgtccggc atcagcttta
     3541 caatggcaat ttcaacaatg cgatcagcgg ccaggtttgt tcccgtggtc tccaggtcaa
     3601 tgacggccag gggttttgtt aattgtaaca tgaatcttta tgggttatgc ggtaatttct
     3661 ttcacaaatg tagcgatatc caaaccatca taattgcccg aactcatcaa cagtaaatta
     3721 ctgttttcgt aggatttcgc tcttaaccag gcggacaatt cctgtaccga actgatcacg
     3781 gcaagccccg gagttccaaa gccctttctg acggtttcgg ggtccagcgg aggcaggcgt
     3841 ttcaattcca gggcatgggc cgaataatag accagggcct ggtcggccgg atccagaacg
     3901 cctttgtatt cgctcatgaa tcccgcctgg agactgctat aggtatgcag ctcgagcacg
     3961 gcgatcaatt cagggtccgg gaactgctct ttcatcgcgg cgatcgttgc ctttactttg
     4021 gaaggagcat gggcaaaatc acggtatacc cgggtgtttc ccttttcccc cagcagctca
     4081 agtcgtttcg atgcgccggc aaaaccggcg atctgttgca gaaaagtttt aagcggcaca
     4141 tccagttccc ggcaaaccag ccaggccgcc tgaagattta acagattatg cgtcccgaaa
     4201 acctgcagtt cctgtgaaat gccttccagc cggaccgaag tgctcccgcc cgccacccgg
     4261 tgctccggaa ttccatatcc tgttttccgg atctccggcg cgacggtctc caccagctct
     4321 ttcagcacgg gatccgtttc gttgtagatc aggagaccgc ccggctcgat catcccgata
     4381 aagatccgga actgctccag ataaaaatca aatgtcggaa atacatttat atggtcccag
     4441 gcgatgccgg taaggatggc gatatgggga aagagaaaat gga
//
LOCUS       DASYCU010000343         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3844197, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000343 DASYCU010000000
VERSION     DASYCU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3844197"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VGM24_06975"
     CDS             complement(<1..468)
                     /locus_tag="VGM24_06975"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583286.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEY4155150.1"
                     /translation="MLTGYPSSIAERFFRYVKIDTQSDPESKTQPSTEKQKVLANLLL
                     EELHATGVTDAELDEYGYIYATIPSNTTKAVPVICFCAHLDTAPDCSGTGVKPILHKN
                     YDGSDLVLPDDPGQIISPQNHPYLSEKRGEDLITASGKTLLGADDKAGVAIIME"
     gene            601..1497
                     /locus_tag="VGM24_06980"
     CDS             601..1497
                     /locus_tag="VGM24_06980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY4155151.1"
                     /translation="MATTAVSKPLISPGWLRVLIFCIVHLLLILTGVLLITAVIHHLK
                     GKTADAEALMKGELLWAVIAMSFAGTMGITYVFRRWVDRKSFLSLGLKYRHHGADMIA
                     GIALAVSMLGLATLILRLTGHLKWTDIIFDKKALMTGLGDLLLVAFYEEILFRGYILG
                     NLLESFNQWVALAISSILFTLFHLGNPGFDFFSIVNIFAIGVLLGMYYLYSRNLWFPI
                     CFHFAWNFLEGPVLGYPVSGIHFDPLLQTEMKGDENITGGAFGLEGSVVVAAVCIIGI
                     LTLYFVLQKKTRPEILPVPDQK"
     gene            complement(1510..1938)
                     /locus_tag="VGM24_06985"
     CDS             complement(1510..1938)
                     /locus_tag="VGM24_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4155152.1"
                     /translation="MAVQSGNTIKVHYNGRLNDGTVFDSSNGRSPLEFTVGTGSVIKG
                     FDEGVQGMQVGEKKTIEIPFLDAYGPVDPSMFIEFPRNQLPEDIQPEVGMQLTMNNAS
                     GEQFPVEVTGVKEDSIVLNANHPLAGKDLIFDLELVEIDD"
BASE COUNT          485 a          496 c          509 g          622 t
ORIGIN      
        1 ttccatgatg atggccactc ccgctttatc gtctgcgccc aataaggttt tacccgatgc
       61 ggtgatcagg tcttcccccc gcttttctga taaatacgga tggttttgcg gagagatgat
      121 ttgcccggga tcatcgggca ataccaggtc ggacccgtca taatttttat ggagaatggg
      181 tttaaccccg gttccgctgc aatcgggcgc cgtatccaga tgtgcgcaaa aacagattac
      241 cggaacggcc ttcgtggtat tggacgggat ggtggcgtat atatatccat attcatccag
      301 ttcggcatcc gtcactccgg ttgcgtgcaa ttcctccagc agaagattgg ccagcacctt
      361 ttgtttttcg gtagaaggct gtgttttgga ttcaggatca ctctgcgtgt caatttttac
      421 ataccggaaa aagcgttctg cgatgctgga aggatatccc gttaacataa tgctttatta
      481 aaaatgaatt ggaaggttaa aaatattgcg tttatcataa gctgccataa aatttctttt
      541 gatcttttta ttccgccttt acgatattcg tattgtgata gttcgaataa tttcctgatc
      601 atggctacta ccgctgtttc caaaccgttg atttctcccg gctggttgag ggtgcttatt
      661 ttctgcatcg tccatttgct gctgatctta accggcgtat tgctgataac ggcggtcatt
      721 caccacctga aagggaaaac agccgatgcg gaggcactga tgaagggaga actgctctgg
      781 gccgttatcg ccatgagttt tgccggaacg atggggataa cctatgtatt ccgccggtgg
      841 gttgaccgga aatctttttt gtcactgggg ttgaaatacc gccatcatgg ggccgatatg
      901 attgccggga tcgcactggc cgtttccatg ctggggctgg ccaccctgat cctgcggctg
      961 accggtcatt tgaaatggac ggatattatt ttcgacaaaa aggccctgat gaccggactc
     1021 ggagatctcc tcctggttgc tttttatgaa gaaatccttt tccggggcta tatcctcggc
     1081 aatctcctgg aatcatttaa ccaatgggtc gctttagcca tatcctctat tttgttcacg
     1141 ctgtttcatc tgggcaaccc gggatttgat ttcttctcca ttgttaatat ttttgcgatc
     1201 ggcgtcctgc tgggcatgta ttatttatac agccgaaatc tctggtttcc catttgtttt
     1261 catttcgcct ggaatttcct ggaaggtcct gtactgggat atccggtaag cggcattcat
     1321 ttcgatcctt tgttgcagac ggaaatgaag ggcgacgaaa atatcaccgg gggcgcattc
     1381 gggctggaag gatccgtggt ggtggccgcc gtatgtatta ttgggatact gaccctttat
     1441 tttgttctgc aaaaaaagac cagaccggaa atcctgccag tgccggatca gaaataacgc
     1501 tgccgggaat taatcatcaa tctccaccag ctccaggtcg aaaataagat ccttgccggc
     1561 taagggatga ttggcattca atacgatgga atcttccttt actccggtca cttcaaccgg
     1621 gaactgttcc cctgaagcat tattcatggt gagctgcata ccgacttccg gctgaatatc
     1681 ttccggcagt tgatttcttg ggaattcaat gaacatggat ggatctaccg gtccatacgc
     1741 atcgagaaaa ggaatttcaa ttgttttttt ctcaccgacc tgcataccct gcacgccctc
     1801 atcaaatccc ttgatgacgg atccggtgcc aacggtaaac tccagcgggg aacggccgtt
     1861 ggaggaatca aagacggttc cgtcgtttaa acggccatta tagtgaactt taatcgtgtt
     1921 accggactga acggccataa gaatgatttt agtttataat tggactgcaa aaatacgatt
     1981 tatttggggg atggatgatc tgtcaaccgc ccgctattcc ccatccgcta tatttgcaat
     2041 atgcagccag atcaacctta tccccggatc attttcatgg gaacgcctga ttttgccgtc
     2101 gcgtccctgg ag
//
LOCUS       DASYCU010000344         5638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2974361, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000344 DASYCU010000000
VERSION     DASYCU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2974361"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VGM24_06990"
     CDS             complement(<1..944)
                     /locus_tag="VGM24_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HEY4155153.1"
                     /translation="MRKLLFLPLMFLAAGVCAQSAYPEDGMLAKIRNEGMNHSQVMDI
                     AFYLTDANGPRLMQSPGYFRAANWARTKLSSWGLQQVKLEPWGHWGRGWELEKFYFAM
                     TAPYYKPLIGFPKTFIPGTQGLQHASVILLDTRDSAGLEKYKGRLKDKIVLLVRNDSL
                     PLGFSPDAHRYTDAELKQLAAFDPKAVVAASGPDKTAEAYRAAAKLLADAKEFSKREG
                     AICMLSSSPRNSDGTVFVQNGTIYSPETPETFNDLAVSYEDYMTIQRLLQHQIPVEID
                     LELRTKVYDQDQQGYNVIGEIPGTDKKLKDELVMIGGHL"
     gene            complement(1013..3367)
                     /locus_tag="VGM24_06995"
     CDS             complement(1013..3367)
                     /locus_tag="VGM24_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4155154.1"
                     /translation="MIKPFLLLFLLLSLLSASAQKRFTVTGYVLDSLSSETLIGASLT
                     VNGAGKGIISNGYGFYSLTLPEGHYTLSVSYVGYQTVRTEIGLDKNISLNFLVNPVAT
                     SSEEVVVYAKKRDANVKNAQMGKIDLAMAQVKSLPVLFGEVDILKIIQLLPGVSNAGE
                     GNTGLYVRGGGPDQNLILLDDAIVYNTGHLFGFFSIFNGDAIKNTSLIKGGMPAQYGG
                     RLSSVLDISMKEGNMNKFEVDGGLGLIASRLSIQGPIKKDKASFIISARRTYLDLLIK
                     PLIPKSSSYSGSGYFFYDLNTKVNYKFSEKDRLYLSGYFGRDVFDFKNAKRSFSTNVP
                     WGNTTATLRWNHVFNRKLFANTTLLYNDYRFAFNGAQDNFQLSLASGIHDLTVKTDFD
                     YFISPKHKVRFGGLFTHHTFTPNILTGHQDSTQFVPNNSNKKYAREMALYLQDDWQIS
                     DRLQLNAGLRWSGFEQTGPYRIYQEDADGNKTDSTDYSRGKTIKAYGGFEPRVTLRYS
                     IDGETSLKASASRNLQYIHLVSNAGTTLPTDLWVPSTYRVQPQTSWQYAAGIFRNFHN
                     NVYETSVELYYKRMDHQIEYKEGYTPSLSDPENAFVFGKGRSYGAEFYINKARGRLTG
                     WIGYTLSWTWRIFPDLNNGNRFPAKYDRRHDLSVVASYELSKKWKLSGVFVYANGNAT
                     SYPEKFYVVEGTLAQDYSRINQYRLPAYHRLDLSAVYTPQHKKNKKVQGSWVFSIYNV
                     YSRLNPYFIYFDQTGSPYDGTLQVQAKKVSLFPILPSVTWNFHF"
     gene            complement(3373..3993)
                     /locus_tag="VGM24_07000"
     CDS             complement(3373..3993)
                     /locus_tag="VGM24_07000"
                     /inference="COORDINATES: protein motif:HMM:NF023255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HEY4155155.1"
                     /translation="MKKALSLVVVLAAALAITYLLLHKRSSSGAPEKRDAPLAVAAGS
                     SAFNRSFTLIMNNYYELSDAFVNWDTAAIREKANKLSSSVDSLKLNELKADTAILQTA
                     DNLAQSIRGEIAGLNGEADIEQKRREFYMVSDILYNLIRTVRYDGATVYHMECPMAFS
                     DSSEAYWLSASSKVVNPYLGKKHPVYKDKMLDCGKVNDSIHFAIEP"
     gene            4073..>5638
                     /locus_tag="VGM24_07005"
     CDS             4073..>5638
                     /locus_tag="VGM24_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY4155156.1"
                     /translation="MLAGLQNVRFEFGARTILEESTWHIQPSERIGLIGYNGTGKSTL
                     LKLLAGEYSPSAGTVERSRSTSVGYLHQDLLSFDTGDSILQVAMGAFEKVLDLEKELE
                     ELARELESGIHGEGAQADELLTRYTDKLHEMDLLDGYSIHHKTEEVLHGLGFTNADLQ
                     RPFREFSGGWRMRVLLAKMILQKPDLLLLDEPTNHLDLPSIEWLEKYLQHYQGSVVIV
                     SHDKFFLDRMVTEIVELYQQNLHFYKGNYSYYEKEKEMRISLQQRAFENQQDYIRQQE
                     KFIERFRAKATKAAAAQSAMKRLEKMDRIENVEMERPDMRIQFRVGKTPGKVLVELKD
                     LSKRFGEIEILNHAEAEIDRGDKIALIGANGKGKSTVLRIIAGTEPFTGTRNWGHNVE
                     DSFYAQHQLESLDVENTVLEEMKMCGSQKNELELRTLLGCFLFSGDDVDKKIRVLSGG
                     EKARVALAKTIAGKANFLLLDEPTNHLDIHSCELLADALNKYEGSYILVSHDRYFISK
                     TANKIWEIDNGKIK"
BASE COUNT         1437 a         1328 c         1453 g         1420 t
ORIGIN      
        1 agatgtcccc cgatcatgac cagttcatct ttaagctttt tatccgttcc cggaatttcg
       61 ccgatcacat tgtacccctg ctggtcctgg tcatagactt ttgtgcgcag ttccagatcg
      121 atttccaccg ggatctgatg ctgcagcaaa cgctggatgg tcatataatc ttcgtaggaa
      181 acggccaggt cattgaatgt ttcgggtgtt tccggagaat aaatggtgcc attctgaaca
      241 aataccgtcc cgtcagaatt cctggggctg ctgcttaaca tgcaaatagc cccttcccgt
      301 tttgaaaatt cctttgcgtc cgccagcaat ttagctgccg cgcggtaggc ttccgctgtt
      361 ttgtcgggac cggaggcagc aacgaccgct ttgggatcaa atgccgcgag ttgttttaat
      421 tctgcgtccg tgtaccgatg tgcatccggg ctgaacccca gcggcaggga atcgttcctg
      481 accagcagaa caattttatc tttcagcctg cccttatatt tttccaatcc cgcggaatcc
      541 cgggtatcca gcagaatcac agaggcatgc tgtaatccct gggtgccggg tataaacgtt
      601 tttggaaaac cgatcagtgg tttataataa ggagccgtca tcgcaaaata aaatttctcc
      661 agttcccatc ccctgcccca atgcccccag ggttccagct ttacctgctg caaaccccag
      721 gacgatagtt tggtcctggc ccagttggcc gcccggaaat atccgggcga ctgcatcagc
      781 cggggaccgt ttgcatcggt cagataaaac gcaatatcca tcacctggga atggttcatc
      841 ccctcattcc ggatcttagc gagcatgcca tcctccggat acgctgactg ggcgcagaca
      901 ccggcggcca gaaacatcag gggcagaaac aaaagctttc tcataataaa atgttttctt
      961 cctgcgaatt tataaaagga aggatcaccg gatcgtttta ttttgacgga aattaaaaat
     1021 gaaagttcca ggtaacggag ggtaatatgg gaaacaggga gactttttta gcctgtacct
     1081 gcagggttcc gtcatagggg cttcccgttt gatcgaaata aatgaaatac ggattgaggc
     1141 ggctgtacac attatagatg ctgaacaccc agcttccctg gaccttttta ttctttttat
     1201 gctggggggt ataaacggcc gagagatcca gccggtggta ggccggcaga cggtattgat
     1261 tgatccggct gtagtcctgc gccagtgttc cttccaccac ataaaatttt tcaggataag
     1321 aagtggcatt tccgttggca taaacaaata ctccggagag cttccatttt ttactcagtt
     1381 catagctggc tactacggaa agatcatggc gacggtcgta ttttgccgga aaccggttgc
     1441 cgttgttcag gtcgggaaat attctccagg tccaggataa ggtatatccg atccagccgg
     1501 tgagcctgcc tctggcctta ttgatataga attctgctcc gtaactgcgg cctttaccaa
     1561 acacaaacgc attttcggga tcgctcagcg acggggtata gccttccttg tattcgatct
     1621 gatgatccat gcgcttataa taaagctcca cggaagtctc gtatacattg ttgtgaaagt
     1681 tcctgaatat accggcagca tattgccagc tggtttgcgg ttgcacccgg tacgtgctgg
     1741 gaacccagag gtccgtcggc aaggtggttc cggcattgct taccaggtga atgtattgaa
     1801 gattacgcga agcggaagcc tttaaggaag tttccccgtc aatggaataa cggagtgtga
     1861 ccctgggttc aaagccgcca taagccttaa ttgtttttcc gcgcgaataa tcggtgctgt
     1921 ccgttttatt cccgtccgca tcttcctggt aaatgcggta gggaccggtt tgttcaaacc
     1981 cgctccatct tagtcccgca ttcaattgaa gccggtcact gatctgccag tcgtcctgca
     2041 gatacagcgc catttcccgg gcatatttct tatttgaatt attgggaaca aattgcgtgg
     2101 agtcctgatg accggtcaga atgttggggg taaaagtatg atgagtgaaa agaccgccga
     2161 agcggacctt atgcttcggg ctgataaaat agtcaaaatc cgttttgacc gtcaggtcgt
     2221 ggatgccgga agccaggctc agctgaaaat tatcctgcgc gccattgaaa gcgaaccggt
     2281 agtcgttata aagtaaagtg gtattggcaa agagtttccg gttgaataca tggttccatc
     2341 gtaaggtggc cgtggtattt ccccagggaa cattggtgct gaaggatcgc tttgcatttt
     2401 tgaaatcaaa gacgtccctt ccgaaataac cgctcaggta gagcctgtct ttttccgaga
     2461 atttataatt cacttttgtg ttcaggtcgt agaaaaaata tcccgagccg ctgtaactgc
     2521 tggacttcgg aatcagtggt tttatcagga gatcgaggta ggttctccgg gcggagataa
     2581 taaaggaggc tttgtccttt ttgatcggcc cctgtatgga taagcgggag gcgatcagtc
     2641 cgagaccgcc gtccacttcg aatttattca tgtttccttc cttcatggaa atatccagaa
     2701 cggaagataa gcggccgccg tactgggccg gcattccgcc tttgatcagg ctggtgttct
     2761 tgatggcgtc cccgttaaaa atagagaaga aaccaaagag gtggcccgta ttgtacacaa
     2821 tggcgtcatc cagcaggatc aggttctgat ccggtccgcc accccgcaca tagaggccgg
     2881 tgttcccttc cccggcatta ctcacgcccg gcaagagctg gatgatcttg aggatatcca
     2941 cctcgccaaa taaaacgggc agggatttta cctgggccat ggccaggtca atcttgccca
     3001 tctgcgcatt cttcacattg gcatcccttt tctttgcgta taccaccacc tcttcgctgg
     3061 atgttgccac cggattgacc aggaaattga ggctgatgtt tttgtcgagg ccgatttcag
     3121 tccttacggt ttgatatcct acataagaaa cagagagggt gtaatggccc tcgggaagcg
     3181 taagggaata aaatccataa ccattgctga tgatcccctt tcccgctccg ttaaccgtta
     3241 aggaagcgcc gatcagggtt tcggaggaaa gagagtccag cacatagccc gtaaccgtaa
     3301 atcttttctg tgcagaggca gaaagtaggg ataataaaag gaacagaagc aggaatggtt
     3361 taatcatgat cgctaaggct caatggcaaa atgaatggag tcatttactt tgccgcagtc
     3421 cagcattttg tctttatata ccgggtgttt tttgccaaga taaggattaa ccaccttgct
     3481 gctggcactt aaccagtagg cttccgagga atcgctgaaa gccatgggac attccatatg
     3541 ataaacggta gcgccatcgt aacggacggt gcggatgagg ttatataata tgtccgaaac
     3601 catatagaat tctcttcttt tctgttctat atccgcttcc ccgttcaatc ccgcaatctc
     3661 gccccggatg gattgagcca aattatcggc cgtctgaagg atggcggtat ccgccttaag
     3721 ctcattcagt ttaaggctgt cgaccgatga cgaaagctta tttgcttttt ctcttatggc
     3781 tgcggtatcc cagttaacaa acgcgtcgct gagctcgtaa taattgttca tgatcagggt
     3841 aaaagaccgg ttaaacgcag aactgccggc agcaacagcc aggggagcat ctcttttttc
     3901 aggagccccg gaagagcttc ttttgtgcag gagcaggtag gttattgcaa gcgcagccgc
     3961 cagaaccaca accagtgata gggctttctt catgaatgga atttaatcaa agataattgt
     4021 aaaaacattt gttttacttt tgcgggtttg atttaacctc catcgcaaaa ttatgttagc
     4081 aggactgcag aatgtacgat ttgaatttgg cgcccggacg atcctggaag aatccacctg
     4141 gcatatacaa cccagcgaac ggattggcct gatcggatat aacggaacag ggaaatccac
     4201 tttgttaaaa ttactggcag gcgaatattc cccaagcgcg ggaacagtgg aaagaagccg
     4261 gagcaccagc gtcggttacc tgcatcagga tcttctgagt ttcgacacgg gggactccat
     4321 cctgcaggtg gctatgggtg catttgaaaa agtgctggac ctggaaaagg agctggagga
     4381 acttgcccgg gagctggaat cgggaatcca cggcgaagga gctcaggccg atgaattatt
     4441 gacccggtat accgacaagc tccatgaaat ggatttgctg gacggttaca gcattcatca
     4501 taaaactgaa gaagtgttgc atgggctcgg gtttaccaat gccgacctgc aacggccttt
     4561 ccgcgaattc agcggaggct ggcggatgcg ggtgctgctt gcgaaaatga ttcttcagaa
     4621 accggacctc ctgctgctgg atgaaccgac caatcacctg gatctgcctt ccattgaatg
     4681 gctggaaaaa taccttcagc attaccaggg atcggtggtt attgtgagtc acgataaatt
     4741 ttttctggac agaatggtta cggaaattgt ggaactttac cagcagaacc tgcacttcta
     4801 taaagggaat tattcttact acgaaaaaga aaaggaaatg cggatctccc tgcagcagcg
     4861 ggcttttgaa aaccagcagg actatatccg ccagcaggag aagtttattg agcgcttccg
     4921 ggcaaaagcc accaaggcgg ccgccgcgca gtcggccatg aaacggctgg aaaaaatgga
     4981 ccggatcgaa aatgtggaaa tggaaaggcc cgatatgcgg attcagttcc gggtcggtaa
     5041 aacgccgggc aaggtgctgg tggaattgaa ggacctcagc aaacgcttcg gcgaaattga
     5101 aattttaaat cacgcagaag ccgaaatcga ccggggggat aaaattgcgc tgatcggcgc
     5161 caatggaaag gggaagtcca cggttctgcg gatcatcgcc ggcacagaac cgttcaccgg
     5221 cacgcgcaac tggggccata atgtggaaga cagcttctat gcacagcacc agctggaatc
     5281 cctggatgtg gagaataccg tgctggagga aatgaaaatg tgcggaagtc agaaaaatga
     5341 attggaattg cgcaccctgc tgggttgctt tttattttcg ggagacgatg tggacaaaaa
     5401 gatccgggtt cttagcggcg gagagaaagc gcgggtggcg cttgccaaaa ccattgccgg
     5461 caaagccaat tttttgctgc tggacgagcc taccaatcac ctcgatatcc attcctgtga
     5521 gttgctggcc gatgccctga ataaatacga gggcagttat atcctggtga gtcacgaccg
     5581 ttatttcatt tccaaaacgg ctaataagat ctgggagata gacaatggaa aaataaag
//
LOCUS       DASYCU010000345         6119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4425617, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000345 DASYCU010000000
VERSION     DASYCU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4425617"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VGM24_07010"
     CDS             complement(<1..934)
                     /locus_tag="VGM24_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY4155157.1"
                     /translation="MSRQPATLFDKVWDSHVVRKIEGGPDVLFIDRHFIHEVTSPVAF
                     LEMENRGLRVIFPEKTFATADHNTPTTNQHLPVRDPLSANQLGALERNSAKYGISHWG
                     LGNPKNGIVHVVGPENGITQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLSSQC
                     IMQPKPKKMRIKVDGKLGKGITPKDVVLYIISRLTASGATGYFVEYAGEVFENMSMEG
                     RMTVCNMSIEMGARGGMIAPDEACFTYIKGREKAPKGEAWEKALAYWKTLKTDAGAGF
                     DLEYDFKADDIEPMITFGTNPGMGIGIGSRIPASQ"
     gene            complement(1050..1667)
                     /locus_tag="VGM24_07015"
     CDS             complement(1050..1667)
                     /locus_tag="VGM24_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY4155158.1"
                     /translation="MSKKDKRIDGYIAASEGFARPILNHLRKLVHMVCPEVEETIKWG
                     FPHFEYHGQILCSMASFKQHCSFAFWKGNIMADPDKILEKVGKTGMGHFGRITALSEL
                     PADEKLKTYLKEAARLNKEGTRIPKEKSTPAEKKVLVVPDYLKKALAGNKKALRTFEA
                     FSPSNKKEYVDWITGAKTESTRNSRLETALEWMAEGKIRNWKYVR"
     gene            complement(1788..4622)
                     /locus_tag="VGM24_07020"
     CDS             complement(1788..4622)
                     /locus_tag="VGM24_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4155159.1"
                     /translation="MKCLFFRNIGGNTYVRSFLIRPLLLLAGCVFLLSGAHAADRYWV
                     AASAGNWNNSANWSNSSGGTAGASVPVLSDRAFFDGGGPGDCTMDIAVGINRITVDAT
                     YTGTISQGANPISIAGIANFGGGIFSGGSADISVAGTFTNSGSAFTSTSGVLQLNGNT
                     AFTGGTFAHHNGTVRIDGISGAISLSGISPSFYVLELVGEARTYNFTSVGNITVSHAL
                     NISGSLFCRINSGSFDVTGDINITNTATGGGGTALINIDGTGTQNVAGGSAAGLGALP
                     QLNINKTGTLNLSGFPSVANDFIYTAGTVSSGSSTFCFIRSTVNSYTITGSLGLGNVL
                     FSAEPTNLTVTLSTGTMLTVNGTLTTAGTANLNINSAVPNTTALMVKGDISIGNSGSG
                     GGGTAGMLINGTGNQTLSGPGAAGQGKLPQISIQKTSGTLLLSGIISEGQDWTYISGS
                     VDAVSNASTVVFGGSGLGITSDGMSFYHVMVNANTITLLNDLTVLGDLTISGGTLSAG
                     TNTINLAGNWNNTGGNFSEATSTVNFNGSGLQSIAYTAGENFYNLNVNNTGAGIRLMN
                     DVSIRNTFNMNQGNTDLNGNALTLGISSTNRGTLVRTSGSLINAGSFTRWFNNVTIPD
                     GSITGLFPMGNATDYRPFYISAPVSRPTGGGTVTVAYTDAGGNSAVSIPDGLFTVSIR
                     KNLYWTMSTANGLAGGTYNLVAQGSGFGQVGDINDLRLTLSNGVVGTAGTNAGTTANP
                     QVNRTGLSLADLANSFYIGSKDILISTLPVNLVSFTATALKDRVRLDWETAGEIDNDH
                     FTIQRSGNAKDWEDILTVSGSVTTSAKSDYTAYDEHPLSGTSYYRLEQTDRDGKETFS
                     FVREMALQKQLLIRLYPNPATDYIFISGLPGGEKLKTELFDHIGQRMNIPVRDQGTDA
                     MLNLSRMAPGIYYIRISQGNSKETRTIVKE"
     gene            4896..5300
                     /locus_tag="VGM24_07025"
     CDS             4896..5300
                     /locus_tag="VGM24_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155160.1"
                     /translation="MQKMIDSSYRPGLGEFMSGIQLHHEKLWFAGKNENWKLADFEIN
                     EIKETITDINNYCKDRPEIHFLPMINPVLDSLANAIQEKNSASFIKNYSLLTGTCNSC
                     HQATKHEFNVIKVPRVSAFSDQEFTVPRQTGN"
     gene            5376..5906
                     /locus_tag="VGM24_07030"
     CDS             5376..5906
                     /locus_tag="VGM24_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HEY4155161.1"
                     /translation="MQDQYLESAKKQFNQYKLLGEKAIRQIPEEKLFWEYNPESNSVG
                     IIVKHLWGNMRSRFSDFLTTDGEKPWRHRDSEFQNDIKTREELLGKWNEGWKCLFEAL
                     EPLTGEDLQKTIYIREEKHSVMEAINRQLTHYAYHVGQIVFLGKMICGQDWKTLSIPK
                     GKSEIFNAEKSSTPKQ"
     gene            complement(5931..>6119)
                     /locus_tag="VGM24_07035"
     CDS             complement(5931..>6119)
                     /locus_tag="VGM24_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155162.1"
                     /translation="FESFEAAESFFKNAGFKKLKSARADHGKLSALPHLIGTASAEQE
                     KKLQAAGKIQETWMLEAE"
BASE COUNT         1496 a         1614 c         1463 g         1546 t
ORIGIN      
        1 cctggctggc cggaatccgg ctgccgatgc cgatccccat gcccggattc gtgccaaacg
       61 tgatcatcgg ttctatgtcg tctgccttga aatcatattc cagatcaaag ccggcgccgg
      121 catccgtttt cagcgtcttc cagtaggcaa gcgctttctc ccaggcctct cctttcggag
      181 ctttttcccg gcctttgata tacgtgaaac aggcttcatc aggtgcgatc attcctccgc
      241 gcgcgcccat ttcaatactc atattgcaaa ccgtcatccg gccttccatg ctcatatttt
      301 caaacacttc gccggcatat tccacaaaat aacccgtggc ccccgaagcc gtcagccggg
      361 atatgatata caggaccaca tctttcggcg taatgccctt gcccagctta ccatccacct
      421 tgatgcgcat tttctttggc ttgggctgca tgatgcattg ggacgaaagc accatctcca
      481 cttcggaagt gccgatccca aaagcgatcg cgccaaatgc accatgggtg gaggtatggg
      541 aatcgccgca gacgatcgtc atgccgggtt gggtgatccc gttttccgga ccaaccacat
      601 gcacgatgcc gttcttcgga ttccccaggc cccagtggga gatgccatat ttagccgaat
      661 ttctttccag cgcacccagc tggttggcgg aaagcgggtc gcggacaggt aaatgctggt
      721 ttgtcgtcgg tgtattgtga tcggcggtgg caaaggtctt ttccggaaaa atgacccgga
      781 gaccgcggtt ctccatctcc aggaaggcca ccggactggt aacttcatga ataaaatgac
      841 gatcgataaa taacacgtcc ggaccgcctt cgatcttgcg gactacgtgc gaatcccata
      901 ctttgtcaaa taaggtagcg ggctgcctgc tcatttcaat aatttataaa ttggctgcaa
      961 aattcttgaa atatatccat ataccctgca ttcttcctgt atattttcta atcaataagc
     1021 taaaaaacat aacaaatgga agaaaccggt taccggacat atttccaatt cctgattttc
     1081 ccttcggcca tccactcgag ggccgtttcc aggcggctgt tccgcgtgga ttccgttttt
     1141 gccccggtta tccagtccac atattctttt ttgttcgaag gactgaaggc ttcgaaagtc
     1201 cttaatgctt ttttgttccc ggccagcgct ttttttaaat aatccggcac gaccagcact
     1261 ttcttttcgg ccggtgttga cttttccttc ggaatccgcg tgccttcctt atttaatctg
     1321 gccgcctctt tcagatacgt tttgagcttt tcgtccgcag ggagctccga aagagccgtt
     1381 attctaccga aatgccccat ccccgtttta ccgactttct ccaggatttt atccggatca
     1441 gccatgatat tgcctttcca gaaagcaaac gaacaatgct gcttgaaaga agccatgctg
     1501 cagaggatct ggccatggta ttcaaaatgc gggaagcccc atttgatcgt ttcttccacc
     1561 tccgggcaga ccatatgaac cagtttgcgc aaatgattca gaatgggcct ggcaaagccc
     1621 tcggaagcgg cgatatagcc gtcgatccgt ttatcttttt tactcatccg gatgaagttt
     1681 attggtttcc tttcccaagc acaaagatcc gtaaaagggc ggatttgccc accggcaaat
     1741 taatttgcgg aaccgaccct ttggggatta aaaaggaaag cggtggatta ttcttttact
     1801 attgtgcggg tctcttttga attcccctgg ctgatccgga tataataaat accgggggcc
     1861 atgcgggaaa gattcaacat tgcgtccgtg ccctggtctc tgaccggaat attcatcctt
     1921 tgcccgatat gatcaaacag ttcggtcttt aatttttctc cgccgggcag gccggaaatg
     1981 aaaatataat ccgttgcggg attcggatat agccggataa gcagctgctt ttggagagcc
     2041 atttcccgga cgaaggaaaa agtctctttg ccatccctgt ctgtttgctc caggcggtaa
     2101 taggaagtgc cggaaagcgg atgctcatca tatgccgtgt agtccgattt cgcactcgtg
     2161 gtcaccgatc cgctgaccgt aagtatatct tcccagtcct ttgcatttcc cgaacgctgg
     2221 atggtaaaat gatcgttgtc tatttcaccg gcggtttccc aatccagcct gaccctgtct
     2281 ttcagggctg tggccgtaaa ggaaaccagg ttcaccggca gggtggaaat caaaatgtcc
     2341 tttgatccga tataaaaaga gttggccagg tcggccagcg acaaaccggt tctgttcacc
     2401 tgcgggttgg ccgttgtacc tgcgtttgtg ccggctgttc ccaccacccc attgctcagg
     2461 gtcaaccgca gatcattgat atctccgacc tggccaaaac cactgccctg ggcaaccagg
     2521 ttataggttc ctccggccag tccgtttgcg gtggacatcg tccagtacag gttttttcgt
     2581 atactcacgg tgaagagacc gtccgggatg ctcacggcag aattaccgcc ggcatcggta
     2641 taggcaacgg ttaccgtgcc gccgccggtg ggcctgctta ccggtgcaga gatataaaat
     2701 ggccggtaat cggttgcgtt ccccatggga aacagaccgg ttattgaacc gtccggaatg
     2761 gtaacgttat tgaaccaacg ggtgaaagat cctgcattga ttaaacttcc gctggttcgg
     2821 acaagcgttc ccctgttcgt ggaggaaatg cccagggtaa gcgcattccc gttgaggtcg
     2881 gtatttccct ggttcatatt gaaggtattc cggatactga catcgttcat cagccggatg
     2941 cccgcaccgg tattattcac attcagattg taaaaatttt cacctgccgt ataggcgatg
     3001 gattgcaaac cggatccatt gaaatttacc gtactcgttg cctcggaaaa attccctccg
     3061 gtattgttcc agttccccgc caggttgatc gtgttcgtac cggctgataa agtacctccg
     3121 ctgattgtca ggtccccgag caccgtcaga tcattcaata aggttatggt attggcattg
     3181 accataacat gataaaaact cattccgtca ctggtaatac cgagtccgct cccaccaaag
     3241 accaccgtgg aggcattgct gaccgcatct accgatccgc tgatataggt ccagtcctgc
     3301 ccttcggaaa taatcccgct taataaaagg gttccggacg ttttctgaat gctgatctgg
     3361 ggaagtttgc cctgaccggc tgcacccgga ccggacaggg tctggtttcc tgttccattg
     3421 atcagcatgc cggcagttcc tcccccgccg ctgcccgaat ttccgatgct gatatcccct
     3481 tttaccatca gcgccgtcgt attgggcacg gcggaattga tattcaggtt ggccgttccg
     3541 gccgtcgtca gggttccgtt caccgtgagc atggttccgg ttgacagggt gaccgtcagg
     3601 ttggtcggtt cagcactgaa caacacattg cccaaaccca ggctgccggt gattgtgtat
     3661 gaattcacgg tggaacggat aaagcaaaaa gtggaacttc cgctggaaac cgttccggcg
     3721 gtatagatga aatcgttcgc caccgaagga aaacccgaca ggttaagcgt cccggtcttg
     3781 ttgatgttga gttgcggaag ggctcccagg cccgcagcgg atccgccggc aacgttctgc
     3841 gtaccggttc cgtcgatatt gatcagcgcg gttccaccgc ctcccgtcgc cgtattggta
     3901 atattaatat cgccggtaac atcaaagctg ccgctgttga tccggcaaaa tagactcccg
     3961 cttatattga gcgcatggct tacagtgata ttgccaacgg aagtaaaatt ataggtcctc
     4021 gcttctccca ccaattccag tacataaaaa gaaggactga tccccgacaa gctgatggcc
     4081 cctgaaatgc cgtcaattcg taccgtaccg ttgtggtggg cgaatgttcc gccggtgaat
     4141 gccgtattac cgttcaattg aagaacaccc gatgtggaag tgaatgcgga accggaattg
     4201 gtgaatgtgc ccgcgacgct gatgtccgcc gaaccgccgc tgaaaatgcc tccgccgaaa
     4261 ttggcgatcc cggcaatgga aatgggattg gctccctggc tgatggtgcc ggtataggtg
     4321 gcatctacgg tgatgcggtt tatcccaacc gctatatcca tcgtgcaatc tccggggccg
     4381 ccgccgtcaa aaaatgcccg gtcactcagg accggaacgc ttgcgccggc cgttccgccg
     4441 gaggaattcg accagttcgc gctgttattc cagttccctg ccgaagccgc cacccagtaa
     4501 cggtctgccg catgcgcgcc cgaaaggaga aatacacacc ccgccagcag caggagtggc
     4561 cggatcagaa aggaacggac ataagtattg ccgcctatgt ttctgaaaaa caggcatttc
     4621 atagccggat accggatttt gtggttaaag aggatatcag actataataa cgtctatcta
     4681 agtgttttca tatatatgcc tcaaaaataa aacctgctaa atagattagc atccgggcgg
     4741 tttcaaccca catcccgttc ttgtattaat tttgggcctg atcaccaccg caaatgaagg
     4801 ccagcctatt tttatgcttt tttgtttttt ctctgctagc ctgcaatcag gagcaggccg
     4861 gctcccaaaa agttttggaa actaaaatag accgtttgca aaaaatgatc gacagttcct
     4921 accggccggg gctcggtgaa ttcatgagcg gcatccagct acaccatgaa aaactctggt
     4981 ttgccggaaa aaacgaaaac tggaaactgg ccgactttga gatcaatgaa attaaagaga
     5041 ccataacgga cattaataat tactgtaaag accggccgga aattcacttc ctaccaatga
     5101 taaaccccgt gctggacagc ctggccaatg cgatccagga gaaaaacagc gcatccttta
     5161 taaaaaatta ttccctcctg accggaacgt gcaatagttg tcaccaggca accaaacatg
     5221 aattcaatgt gataaaagtt ccccgggttt ctgcattttc tgaccaggaa tttactgttc
     5281 cccggcagac cggcaactaa tacccggggc tgaaggttat ccggccttct gaaatgtaat
     5341 ccggcatcgt aaatttggat ttgaaaacag gccatatgca ggatcaatat ctggaaagcg
     5401 caaaaaaaca gtttaaccaa tataaacttc tcggggaaaa agccatccgg cagattccgg
     5461 aggagaaatt attctgggaa tataatccgg aaagcaacag cgtcggaata attgtaaagc
     5521 atttatgggg caatatgcga tcccggttca gcgatttcct gacgaccgac ggtgaaaaac
     5581 cctggcgtca ccgcgacagc gaatttcaga atgatataaa aacaagagaa gaactgctcg
     5641 gcaaatggaa cgaaggctgg aaatgcctct ttgaagccct tgagccgctg accggggaag
     5701 accttcaaaa aaccatctat atccgagagg agaaacattc cgttatggaa gccattaacc
     5761 ggcaactcac gcattatgct taccatgtcg gccaaattgt ttttcttgga aaaatgatct
     5821 gcggtcagga ttggaaaacc ctgtccattc cgaaagggaa atcggaaata tttaatgcgg
     5881 agaaatcttc caccccgaaa cagtaaatcc aggagccatc cgctgtccat ttattctgcc
     5941 tccagcatcc aggtctcctg tatctttccg gctgcctgca attttttttc ctgttccgcg
     6001 gaagccgttc cgatcaaatg aggcaaagcg cttagtttcc catgatccgc cctggccgat
     6061 ttcagctttt tgaaccctgc atttttaaaa aaagattcag ccgcttcaaa actttcaaa
//
LOCUS       DASYCU010000346         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4280549, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000346 DASYCU010000000
VERSION     DASYCU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4280549"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VGM24_07040"
     CDS             <1..260
                     /locus_tag="VGM24_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY4155163.1"
                     /translation="ILLGFAAGAAIGAALGILFAPDKGTETRRRISEKGSDVADSIKD
                     RFSGLVDGVKDKFSNLKAEGEDVAEKGYTAYNRSKSEINAF"
     gene            complement(348..1511)
                     /gene="moeB"
                     /locus_tag="VGM24_07045"
     CDS             complement(348..1511)
                     /gene="moeB"
                     /locus_tag="VGM24_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY4155164.1"
                     /translation="MSLDNISFSKEERARYNRHMIIPDFGEEAQKKLKAAKVMVIGCG
                     GLGSPALLYLTAAGIGTIGIIDFDDVDDSNLQRQVLFDVNEIGKPKVEAAKRRLEALN
                     PHVRFNLYNAHLNSENALEIFKDYDVIADGTDNFPTRYLVNDASVLLDKPNVYASIFQ
                     FEGQVSVFNYVNGRGERGPNYRDLYPTPPPPGLVPSCAEGGVLGVLPGIIGSLQALEV
                     IKVITGVGEPLSGRFFTFDALNFENRTFNIKRRDDNPVNGKNPTITKLIDYEQFCGMR
                     AVEEKPIKEISAKELYDWQVKGKKFQLIDVREPGEYEIVEIGGELIPLATVADQAGRI
                     SRDLDVVVHCKVGGRSAKAIRELEEKFGFRNLYNLKGGILAYIDQVKPELTKY"
     gene            complement(1522..1803)
                     /locus_tag="VGM24_07050"
     CDS             complement(1522..1803)
                     /locus_tag="VGM24_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY4155165.1"
                     /translation="MATVIIPTPLRKFTNQTARIQIPAKTVGEIIDSLTRDFPDLKKH
                     LLDDQGRVRSFVNIFAGDDDIRDLQQEKTVVEESTLVSIVPAIAGGISI"
     gene            complement(1808..2245)
                     /locus_tag="VGM24_07055"
     CDS             complement(1808..2245)
                     /locus_tag="VGM24_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY4155166.1"
                     /translation="MIQIQNPALEKMLEHAKQVFPDECCGFLFGTEDREGNRNILEIT
                     EVNNSKEGDKRRRFEIGPRDYLRGEQYAVEKDLLLLGVYHSHPNHPAIPSEHDRVAAQ
                     PYFSYIIISVMQGNIDHLRSWQLNEENRFEEENTSTIHLHQSI"
     gene            complement(2242..3180)
                     /locus_tag="VGM24_07060"
     CDS             complement(2242..3180)
                     /locus_tag="VGM24_07060"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY4155167.1"
                     /translation="MSDLSTIAIPGIPEKLAALQSQIGNTPLFEVRRLFQHKKVRLYA
                     KQEWMQLSGSVKARAAYAIFRDAIGKGRLGPGKILLDATSGNTGIAYAWIGKALGIPV
                     TLCLPENASAEIKEILKSLDVDLVLTSRFEGTDGAQEVALALAEKNPDRYFYADQYKN
                     ENNWKAHYSGTGPEIIESVPGITHFVAGLGTTGTFMGTGRRLKKYNPAIQLISLEPDN
                     PLHGMEGWKHMETAVIPEIYDPLLADADIEVSTEEAYEMMKRAKEIEGLNLSPSSAAN
                     LAGAIRVASGLEKGIVVTVFPDNADKYADTIKKIFK"
     gene            complement(3388..>4398)
                     /locus_tag="VGM24_07065"
     CDS             complement(3388..>4398)
                     /locus_tag="VGM24_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4155168.1"
                     /translation="EFKPEYTPRLAAVYTFMENHNFRFTYQRGYRFPALFEALSYVNN
                     GRVKRVGSLPYINRGLGYLENSYTQQSVINFNAAVSAQGNSDQAALANRNLLVVANLP
                     DAGPERIISYEAGYKAVLLNNRLVLDLDAYSNQYTGFLGQVQVFVPKGVTVGTDAAVL
                     AMLDRNRDATAATAATAASQGQDRYRVYSNAKNTYHNFGSALAITYNLYRKFTVAGNI
                     NFNEMKTNKTNDIFVTGFNTPRWSTNLSFGNRELVKNFGFNITWRWQDSFLWESPLVT
                     GSVSAYSSVDAQVTFHLPRLKSSIKVGASDIFNHRYIQYAGGPTLGGLYYAAITVDGL
                     LK"
BASE COUNT          995 a         1112 c         1118 g         1173 t
ORIGIN      
        1 aaattttatt ggggttcgca gcgggtgctg cgataggcgc agcattaggt attttatttg
       61 caccggataa gggaaccgaa acccgtcgga ggatctctga aaagggaagc gatgtggccg
      121 attcgataaa agacaggttc agcgggctcg tagatggggt aaaagataag ttttccaacc
      181 tgaaagcgga aggagaagat gtggccgaaa agggatatac tgcttataac cgcagcaagt
      241 ccgaaataaa cgccttttaa aaatattgaa ccataaagaa taatcccccg tcgccggggg
      301 atttctcttt attcttcact tgggaatata attcttccct tacggaatca atattttgtc
      361 agttccggtt tcacctggtc gatataggcc agaattccgc ctttcaggtt gtacaggttc
      421 cggaacccga atttttcttc cagctcccgg atggccttgg cgctgcggcc gcctacttta
      481 cagtgaacga ccacatccag gtcccgggaa atccgcccgg cctgatcggc caccgttgcc
      541 aggggaatga gctcgccccc gatttccacg atttcgtatt ctccgggttc gcgcacatcg
      601 atcagctgga attttttccc cttcacctgc cagtcataca attcttttgc tgagatttcc
      661 tttatcggtt tttcctccac ggcgcgcatt ccgcaaaact gttcatagtc gatgagtttg
      721 gtgatggtcg gatttttccc gttcaccgga ttatcgtccc ggcgtttgat attaaacgtg
      781 cggttttcaa agttcagcgc atcgaaagtg aagaaccggc cggaaagggg ttctcccaca
      841 ccggtaatga ccttaatgac ctccagggcc tgcaaactcc cgatgatccc cggtaaaacg
      901 ccaagcactc cgccttccgc gcagctggga accagtccgg gcgggggtgg ggtcgggtaa
      961 aggtcccggt aattgggtcc tctttctccc cggccgttca cataattaaa tacggaaacc
     1021 tggccttcaa actgaaaaat ggatgcgtac acattgggtt tgtccaataa aacgctggca
     1081 tcatttacga gataacgcgt gggaaaatta tccgttccgt cggcgatgac atcgtaatcc
     1141 ttaaaaatct ccagggcatt ttccgaattc aggtgtgcat tatacaggtt gaaccgtaca
     1201 tgcggattca gcgcttccag ccggcgtttg gccgcctcca ctttgggttt cccgatctcg
     1261 ttgacgtcga ataatacctg tctttgaaga ttgctgtcgt ccacatcatc gaaatcaatg
     1321 atcccgatgg tcccgatccc tgcagccgtc aggtaaagca gggcaggact tcccagtccg
     1381 ccgcaaccga ttaccatcac tttggccgct ttcagttttt tctgggcttc ttccccaaag
     1441 tccggaatga tcatatgccg gttataccgg gcccgttctt ctttactaaa ggatatatta
     1501 tctaatgaca tgatgtttga tttatattga aatgcctccg gcaatggcgg gaacgatgct
     1561 gaccagcgtg gattcttcca caaccgtctt ttcctgctgc aggtcgcgga tatcgtcgtc
     1621 ccccgcaaaa atgtttacaa aggagcggac cctgccctgg tcgtccagca ggtgcttttt
     1681 cagatccggg aaatcgcggg tcagcgaatc gatgatctcc ccgacagtct ttgcgggtat
     1741 ctgaattctg gccgtttgat tggtgaactt gcgaagcggc gtgggaataa taacagttgc
     1801 catattttta tattgattga tgtaaatgaa tggttgatgt attttcttct tcaaagcggt
     1861 tctcttcatt gagctgccag gaccggagat gatcgatatt tccctgcatg acggaaataa
     1921 tgatatagga aaaataaggc tgggcggcca ctcggtcatg ctccgacgga atcgccggat
     1981 ggttggggtg ggaatggtaa accccaagca acaggagatc cttttccacc gcatattgct
     2041 cgccccgcag atagtccctg ggcccgatct cgaatcgcct gcgtttgtct ccttcttttg
     2101 aattgttcac ttctgttatt tccaggatat tccggtttcc ttcgcggtct tcggttccaa
     2161 acagaaatcc gcagcattca tccgggaata cctgctttgc atgttccagc attttttcaa
     2221 gagcgggatt ttgaatctgg atcatttaaa tatcttttta atagtgtcgg catatttgtc
     2281 cgcattatcg ggaaaaaccg tgaccacaat ccctttttcc agtccggagg ctacccggat
     2341 ggcgcccgcg agattggcag cggaagaagg actcagattc aatccttcga tttcttttgc
     2401 ccgcttcatc atctcatacg cttcttccgt ggatacttcg atgtcggcat ctgccaggag
     2461 cgggtcatag atctccggta tcaccgccgt ttccatatgc ttccagcctt ccataccgtg
     2521 cagcggattg tcgggctcca gggaaataag ctgaatggcc ggattgtatt ttttcagccg
     2581 cctgccggtc cccataaacg tgccggtggt tccaagtccc gccacaaaat gagtgatccc
     2641 gggtacggat tcgatgattt cgggtcctgt tccggagtaa tgggccttcc agttgttctc
     2701 gttcttatat tgatcggcgt agaaatagcg gtccgggttt ttttcggcca gggcgagggc
     2761 gacttcctgc gccccatcgg ttccttcaaa ccgggaggtg aggacgagat cgacatccag
     2821 tgatttcagt atttctttta tttccgccga agcgttctcc ggcaggcaaa gggtgactgg
     2881 tattccgagg gccttgccga tccaggcata ggcgatgccg gtattgccgc tggttgcgtc
     2941 cagcaggatc ttgccgggcc ccagccttcc tttaccgatg gcgtcccgga aaatggcata
     3001 cgcggcccgg gctttcacac tgccggacag ctgcatccat tcctgcttgg cataaagcct
     3061 aaccttctta tgctgaaaca gccggcgcac ttcgaaaagt ggggtgtttc cgatctggga
     3121 ctgcagggca gccagctttt ccgggatccc cggaatggcg atggtggata agtcggacat
     3181 ggcgtaaaat aaaaaagctc cacatgggtg gagcttctgt attatttgtc gtttttagtt
     3241 tatatacgaa taccctccac ccgataaaca aggcgttctg cacaacaaca acagctctgg
     3301 agtgtattca gactcatttg catgattctt tactaacaca aagataatcg ttttgttcaa
     3361 taaatggtgg aacatggaaa ttctttctta ttttaataat ccgtccaccg ttatggctgc
     3421 ataataaagg ccgccaaggg tgggtccccc ggcatactga atataccggt gattaaagat
     3481 atcggacgcc ccgaccttga tagaagattt gagcctggga agatgaaaag tcacctgggc
     3541 gtcgacgctg ctgtaagcgg aaacgctgcc ggtcaccagg gggctctccc agagaaagga
     3601 atcctgccat ctccaggtaa tattgaatcc gaaattcttt accagttcgc ggtttccaaa
     3661 agaaaggttg gttgaccagc gcggggtatt gaagccagtg acaaagatat cattcgtttt
     3721 attcgttttc atttcgttga aattgatatt gccggcaacc gtaaatttcc tgtacaggtt
     3781 ataggttatg gccaaggcgg aaccgaaatt atgataggtg tttttagcat tgctgtatac
     3841 gcggtaccgg tcctggccct ggctcgcggc ggtggccgca gtggctgccg ttgcatcccg
     3901 gttcctgtcc agcatggcca gcaccgcggc atccgttccc accgtaacgc cttttggaac
     3961 aaatacctgg acctgtccca gaaaacccgt atactgattg ctgtaggcat ccaggtcaag
     4021 caccaggcgg ttattcagca gtacggcttt atacccggct tcgtaggaga tgatccgttc
     4081 aggtccggca tccggaagat tggctaccac cagcaggttc cggttggcca gtgcggcctg
     4141 atcggaattt ccctgggcac tgaccgccgc attgaaattg atgacggact gttgtgtata
     4201 actattctcc aggtagccca gcccccggtt gatatagggt aggctgccca cgcgtttcac
     4261 ccttccgtta tttacatagg acagggcttc aaagagggcg gggaagcggt aaccccgctg
     4321 ataggtaaaa cgaaaattgt ggttttccat aaaggtatag acggctgcca ggcgcggggt
     4381 gtattcgggt ttgaattc
//
LOCUS       DASYCU010000347         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2394667, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000347 DASYCU010000000
VERSION     DASYCU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394667"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1064)
                     /locus_tag="VGM24_07070"
     CDS             complement(96..1064)
                     /locus_tag="VGM24_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEY4155169.1"
                     /translation="MKRGLFCLCCLFLYNRVFCWGFYMHEQINYHAVFLLPPEMMILY
                     KPNIGFLTEHAVDPDKRRYMLASEGAHHFMDIDHYGRYPFRDLPRNYQAAVEKFSSDT
                     LLAYGIVPWWVQTMLGRLTRAFREKDNRHILKYSAELGHYIADAHVPLHACSNHNGQL
                     TNQQGIHAFWESRIPELFAAQRWDFFIGKAVYIPDPAAFIWARLLESAAASDTVLRCE
                     REAGSGIRPDQKYAFEERNNKLIRQYSTAYATMYNEKLNGMVERRMRQSIFAVASFWY
                     TAWVNAGQPPLDRLSHDSLSLAEQQEYQLLDLEWKKGKVLGRNEEE"
     gene            complement(1112..1732)
                     /locus_tag="VGM24_07075"
     CDS             complement(1112..1732)
                     /locus_tag="VGM24_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY4155170.1"
                     /translation="MKFKSIAGKLFKYFLQGLIILAPITITLWAVLSLFGWIDSILPD
                     FLHRLFPRLALDETGEPRRIPGLGFVVVIGIVLLVGYVSSSFIVSKMVELFDNILEKT
                     PGIKIIYTTIKDFFEAFAGNKRKFDRAVLVSIDAPDIWRIGFVTHDELLEFGLQDYIS
                     VYVPQSYAFAGHLYFVKKERVRLITDISSTDAMKFAISGGVTQLEE"
     gene            1912..2550
                     /locus_tag="VGM24_07080"
     CDS             1912..2550
                     /locus_tag="VGM24_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY4155171.1"
                     /translation="MENREKTESRDRILEKARDLFMQYGIRSITMDEIAGQLGISKKT
                     IYQFFTDKDEMVEAVVMDEIKGNEEGCKQFQIDAENAVQEIFLAMENVQEMMKTMNPQ
                     LMYDLEKHHPAAFRRLKQYKYHFLYHMIRENLERGIKENIYRKDLNTDITCRYRIESA
                     FMAFNQEAFPWNKYPMNQTCHELALLFMHSICSAKGKKLIDKYLNEEQKITL"
BASE COUNT          735 a          612 c          638 g          585 t
ORIGIN      
        1 tttatttgaa ggagccgcaa atttacgaaa accgggctgc cggatgattt tccggaaggg
       61 tttttttgca gctccggtgc tgccggcccc gtaaatcatt cctcctcatt ccgcccgagc
      121 actttcccct ttttccattc caggtcaagc aactggtatt cctgctgttc ggccaggctg
      181 agactgtcgt gactaagccg gtccagcgga ggctggccgg cattgaccca ggcggtatac
      241 cagaaagagg caacggcaaa gatggattgc cgcatccgcc gctccaccat gccgtttaat
      301 ttctcattat acatcgttgc gtaggccgtc gagtactgcc ggatgagttt gttattgcgt
      361 tcttcgaagg cgtatttctg atcgggccgg atgccggaac cggcttcgcg ttcgcagcga
      421 aggaccgtat cggatgcggc tgcactttcc agcaggcgcg cccagataaa ggcggcggga
      481 tccggaatat aaacggcttt gccgatgaag aaatcccatc tctgcgctgc aaataattcc
      541 gggatccgcg attcccagaa cgcatgaatt ccctgttggt tggtgagctg gccgttgtgg
      601 ttactgcagg catgcagcgg aacatgggca tcggcaatat aatgaccgag ttccgcagaa
      661 tatttaagaa tgtgccggtt gtctttttcc cggaaagccc gggtgagcct gccgagcatg
      721 gtctgtaccc accagggtac gattccgtaa gccagcaggg tgtctgaaga gaatttttca
      781 acggccgcct gatagtttct gggaagatcg cggaaggggt atctgccata atgatctatg
      841 tccatgaagt gatgcgcccc ctccgaagcc agcatatatc ttcttttatc cggatcaacg
      901 gcgtgttcgg tcagaaaccc gatattcggt ttatagagga tcatcatttc aggaggaagc
      961 agaaaaaccg cgtgatagtt gatttgctca tgcatataaa aaccccagca aaatacccgg
     1021 ttgtacagga acagacagca aaggcaaaac aaccctcttt tcataaaaag atttgaatgc
     1081 tgctaaccgg atttaaaact gctgaaacac ctcattcctc aagctgcgta acccctccgg
     1141 agatcgcgaa tttcatggca tccgttgaac tgatatcggt gatgagccgc acccgctcct
     1201 ttttaacgaa atataggtgg cccgcaaacg cataggattg gggaacataa acagagatat
     1261 aatcctgcag accgaattcc agcagttcat catgggtgac gaagccgatt cgccagatgt
     1321 cgggagcgtc aatggaaacc aatacggccc ggtcgaactt gcgtttgttt ccggcaaaag
     1381 cttcaaaaaa atctttaatg gtcgtataga tgatcttgat gccgggcgtc ttctccagga
     1441 tattatcaaa aagctccacc atcttgctta cgatgaagga cgaggaaaca tagcccacga
     1501 gcagcacaat cccaatcacc acgacaaacc ccagtcccgg gatcctgcgc ggttccccgg
     1561 tctcatccag ggcaagccgc gggaagagcc ggtgcaggaa atccggcagg atgctgtcga
     1621 tccatccgaa aagcgacagt acggcccaaa gcgttatggt gatcggagcc aggatgatca
     1681 gcccctgcag aaaatattta aacagcttac ctgcaatgga tttgaatttc atgagcagtg
     1741 gtttgtttct caaaagtaaa caaacattcc ccctgggcca tgcttaaaac acccgcggac
     1801 tttccggcat tttcacctga attgtaaaaa aaaactaaaa attataaccg gaaactttgg
     1861 tgacgaaaaa agtttccata gttttgcagc tccaaacgca agggaacaaa aatggaaaac
     1921 agggaaaaaa cggagagcag ggacagaata ctggaaaagg cgagggatct gttcatgcaa
     1981 tatggcatac ggtccattac catggatgag atcgcgggac agctgggcat cagcaaaaag
     2041 acgatctacc agttctttac cgataaggat gaaatggtgg aagcggtggt gatggatgag
     2101 atcaaaggaa atgaagaagg ctgcaaacag tttcagatcg atgcggagaa cgcggttcag
     2161 gagatttttc ttgccatgga aaacgtgcag gagatgatga agaccatgaa tccccagctc
     2221 atgtatgatc tggaaaaaca tcatccggct gccttccgca gattaaagca gtacaaatat
     2281 catttcctgt accatatgat cagggaaaac ctggaaaggg gaataaaaga aaatatttac
     2341 cggaaggacc tgaataccga tatcacctgc cggtaccgga tcgaatccgc tttcatggcc
     2401 tttaatcagg aagcttttcc ctggaataaa tacccgatga accagacctg tcatgaactc
     2461 gccctgctgt tcatgcacag tatttgcagc gcgaaaggga aaaaactgat tgacaaatat
     2521 ttaaatgaag aacaaaagat aaccctatga ataaaaactt cagtatgaaa
//
LOCUS       DASYCU010000348         9443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5002173, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000348 DASYCU010000000
VERSION     DASYCU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5002173"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..2212)
                     /gene="feoB"
                     /locus_tag="VGM24_07085"
     CDS             complement(116..2212)
                     /gene="feoB"
                     /locus_tag="VGM24_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEY4155172.1"
                     /translation="MNKRKIHIGLVGNPNSGKSSLFNALTGLNQKVGNFPGVTIDKKT
                     GSSDLTPGYSASIVDLPGTYSLYPKRSDEWVTYKVLLNQDEEVKVEMVILLVDANNLK
                     RNLLFSSQIIDLKIPVVIALTMMDLAARKGIEIDVPGLERELGVPVVPVNPRKNKGIA
                     QLKKIISQRVQQLSHAPARDFISNRALASDAVSQLKKVFPDISDYTALHYLINHEGLQ
                     FPADQHNRIEGIEEVNQFNHTRVQAEEIMQRYARIKQVMQLTTTEPDPLQKALFTERL
                     DNILLHRVWGYAILLIVLFLLFQSVFWLAQFPMDGLDWTFTKMSTWVSAQLPDGWFRD
                     LMVNGLLAGLSGILVFIPQIMILFGLITVLEDTGYMARISFLTDKLMRKVGLNGKSVM
                     PMISGFACAVPAIMSARNIENKKERLLTILVTPLMSCSARLPVYTMLIALVVPNKEVL
                     GFLNIQGLVMMGLYLMGMVAALIVSYVAKRFIHSREKSFFILELPVYRAPRWKNVGVT
                     MISKAKIFVRDAGKVIIVISMILWGLSSYGPPGRMRAVAARYPAPSEAKAKNAALLEN
                     SYAGILGRTIEPAIKPLGYDWKIGIALITSFAAREVFIGTMATLYSVEGGKNADPQTL
                     RQRMASARTEEGTAVYTLATGVSLMIFYAFAMQCMSTLAVVKKETRGWKWPIIQFIYM
                     TGLAYVMSLLAYQLLK"
     gene            complement(2292..3536)
                     /locus_tag="VGM24_07090"
     CDS             complement(2292..3536)
                     /locus_tag="VGM24_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY4155173.1"
                     /translation="MVLLRHMRFLKAVLLYSISAFGGPQGHLGMMMKTFVNGRKDITE
                     EELMDYNAFCQLLPGASSTQTLTLIGYKRGGIPLAVLTLLIWITPACILMGFFSFFIE
                     FMSQKGSHAELFKFIQPMTVGFLIYASWKAFKISVHNTITHCILLVATVATFFLFKTP
                     WIFPILIVLGGFITNLSSKRIPQVEVLTRKIKWANIWLFAIIFILAGITSEMARKNNW
                     PHRRPINLFENTYRFGSLVFGGGQVLIPLMYEQFVARPESEKLIERNPNIVKIGKDEF
                     YTGAGMVRAIPGPVFSVSSYMGGIAMKDMGIPMQVLGCIIGSIAIFLPSALLVLFFFP
                     IWSNLKKYAAVYRALEGINAVVVGIMVAGSLYMTKDFSFSNNLILGLEVFVTFITFLL
                     LLYSRLPSPVIVFICLLLGWFI"
     gene            complement(3650..4417)
                     /locus_tag="VGM24_07095"
     CDS             complement(3650..4417)
                     /locus_tag="VGM24_07095"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4155174.1"
                     /translation="MLVFLAMQAGAQEVTIRGTVFNMYRTHPLEAVSVISNTGRGTTT
                     DANGNYSIRVNEKDSLSFSYLGRATQMFAVSEMNHTTGFDIALHVNVTELSEVRVAPR
                     DYHMDSLQNRIDYEKSFNYRKPGFKLTDPSSGGLGVGVDLDALINMLKFRQIRRSLAF
                     RERLEEDEQDKFVDHRFNRSLIKRITGLDGDLLDSFMLAYRPSYLFAKSATDYDFLYY
                     IKLADQEFQFNQSNPGIHKESIFKGEDSLKRARIINR"
     gene            complement(4516..5136)
                     /locus_tag="VGM24_07100"
     CDS             complement(4516..5136)
                     /locus_tag="VGM24_07100"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY4155175.1"
                     /translation="MKDNGKQISIALFASGRGSNAREIIRYFHQEEHAVSGHRVRVSL
                     ILCNKPGAGVLEIAQKENIPSLLIEREEFYRGAHYLGELEKKNIRFLVLAGFLWKIPP
                     ELIRAYPDHIINIHPALLPAYGGKGMYGNAVHQAVISAGEKESGISIHKVDEQYDHGQ
                     VLFQARCPVSAEDTAETLAQKIHALEHAHYPEQIARWMESKLSLNP"
     gene            5428..6690
                     /locus_tag="VGM24_07105"
     CDS             5428..6690
                     /locus_tag="VGM24_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4155176.1"
                     /translation="MHYSRLSAFRISYSVLLTSVILLFASVSMAQDGKALFQSNCASC
                     HNPIKVVTGPALKGVTSRVPDQKLLHAWIHNNTAVLASGNKYFNDLYVQFSKTPMNTF
                     PGLSDAEIDAILKYVESYPEPGKTGPSPTGGEQAVPLNDNSLLYGILTLILAVIMLVL
                     MQVNSNLKKLSDDKSGIPAGEPIPFYKNKAYIAMIVLVLFVLGGYFLVQAAIGLGRQQ
                     NYEPVQPIFFSHKVHPGNNQISCLYCHGGAWDSKHAGVPSLNVCMNCHAAINDYHGEP
                     LHRADGSTVDANAEIKKLYSYTGYDPASGKYTSPGKQIEWVKIHNLPDHVYFNHSQHI
                     RAGKVQCQTCHGNITEMNEVKQFAELSMGWCINCHRTTKVNFPDGKGNGNKFYSIYQK
                     FHQDLKSGKMDSVTVENIGGTECQKCHY"
     gene            6779..>9443
                     /locus_tag="VGM24_07110"
     CDS             6779..>9443
                     /locus_tag="VGM24_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEY4155177.1"
                     /translation="MSEKKYWQNFGEYSSSEASRKMSENEFQEELPMEGFDSKGLLDV
                     KTPRRDFLKYLGYSTAAATLAASCKIPVRKVVPYLNKPDNMIPGVADYYASTYVSGGD
                     VTPIVAKVRDGRPIKLEGNELSGLTKGGTSARVQASVLDLYDTARLRYPVQMIKGLPQ
                     EVTSFEAFDKLVSDSMAGQTGSIVLLTSTITSPTSNQVINEFLAKYPGSRHVQYDTDS
                     FSGMLLANEAAYGKRSIPSYRFDNAKVIVSLGADFLGTWLSPVEYARQYATGRKINEK
                     APEMNRHYQFESLLSLTGANADQRYAHKPSETGAVALALLAALGGNVTAPALSDKLKA
                     GIAKAAKDLTAAKGKALVVSGSNDVNVQLIVNAINETVGANGTTINWAAPLLTRQGMD
                     SEFATLVSDLNEGKVGTLLIYDVNPAYDYYDAKKFTDGLQKVKTSVSFNGRLDETTEL
                     CKFVLPSPHFLERWGDAQAKPGYISMIQPVIAPLFLTRPYEESLMKWSGNNQTYDEFF
                     KQYWEGKLGGLEAYEKALQDGVIEPTEMPAAAGGSYNGAKLADAASAISSSKKGSALE
                     LVIYQQVSMGVGSQPNNPWLLELPDPIARSTWDNFAMVSPKFLIDNYDIDLGNQHQAD
                     KFEVHPERPVIKIKANGKETTIPVVAVPGVADGVIAIAVGYGHQSADPKNTASFIGRA
                     VPGAGRNAYPFASFNGTTVDWQATDVSFEKTNEEYQIAFTQTHNSYEGRTSVIKEMNL
                     EEFVKNPNEVLEERENELKHFGGIENFEKEGTLYGVYDKPGIHWGMSIDLNSCIGCGA
                     CVVACNAENNIPMVGKNEVMRFHDMQWMRIDRYFTGDYSDPHSVQTIFQPMLCQHCNN
                     APCENVCPVSATNHSSEGLNQMIYNRCIGTKY"
BASE COUNT         2345 a         2376 c         2535 g         2187 t
ORIGIN      
        1 gcctaccgga tctttaccga ttccttttac gtgaaactga aaccggtggg ggcggtcaat
       61 aatttttacc agacggcttt tgttgtggaa tgatgaccgg gagagaatag ggccgttatt
      121 tcagcagttg gtacgccagc aggctcatta cataagccag tcccgtcatg taaatgaact
      181 gaatgattgg ccatttccat ccccgggttt cttttttcac cacggccagg gtgctcatgc
      241 actgcatggc aaaggcatag aagatcatga gggagacgcc ggtggccagg gtataaacgg
      301 cggtgccttc ttccgtccgg gcggaggcca tgcgctgtcg caaagtttgc gggtcagcat
      361 ttttccctcc ttcaacactg tacaaggtgg ccatggtgcc gataaatact tcgcgggcgg
      421 caaaagaggt gatcagcgcg atgccgatct tccagtcata ccccaggggt ttgatggcgg
      481 gctcgatggt tcttcccagg atgccggcat aggaattttc cagcagggcg gcattcttgg
      541 ccttggcttc ggaaggcgcc ggataacgcg ccgccacggc gcgcattcgg cccggcggtc
      601 cgtagctgct gagtccccag aggatcatgc tgatcacgat gatcactttt ccggcatccc
      661 gcacaaatat ctttgccttg ctgatcatgg taacgcccac gtttttccag cggggggcgc
      721 ggtacacggg cagttccagg atgaaaaagc ttttttccct ggaatggata aagcgtttgg
      781 ccacatagga aacgatcagg gcggctacca tgcccatcag gtataatccc atcatcacca
      841 ggccctgtat attcaggaat ccgagcactt ctttattggg aacgaccagg gcgatcagca
      901 tagtataaac gggcagcctg gcgctgcagc tcatcagggg cgtgacgagg atggtgagca
      961 gccgctcttt tttgttttca atattcctgg cgctcatgat ggcgggcacg gcgcaggcga
     1021 aaccgctgat catcggcatc acgcttttgc cgttcagtcc cacttttcgc atgagcttgt
     1081 cggtgagaaa gctgatgcgg gccatataac ccgtgtcttc cagcacggtg atcagcccga
     1141 acagaatcat gatctgggga ataaacacca ggatgccgct caggcctgcg agcagtccgt
     1201 tcaccatcag gtcccggaac cagccgtccg gcagctgggc ggaaacccag gtgctcattt
     1261 tggtaaaggt ccagtcgagc ccgtccatgg ggaactgcgc cagccagaac acgctctgga
     1321 aaagcaggaa gagcacaatc agcaggatgg catagcccca cacgcggtgg agcaggatat
     1381 tgtcgagccg ttcggtgaac agggctttct ggagcggatc gggttcggtg gtggtcagct
     1441 gcatcacctg tttgatccgg gcgtagcgct gcatgatctc ttcggcctgc acccgggtat
     1501 ggttgaactg gtttacttct tcaatgcctt cgatccggtt gtgctggtcg gcgggaaact
     1561 gcagcccctc gtggttgatg aggtagtgca gggcggtgta atcgctgata tcgggaaaga
     1621 cctttttgag ctgggaaacg gcatcgctgg ccagggcgcg gttcgaaata aaatcccgcg
     1681 ccggggcgtg ggacaattgc tggacccgct gggaaatgat ctttttcaat tgggcgatcc
     1741 ccttgttctt ccggggattc acgggaacga cgggtacccc gagttctctt tccagcccgg
     1801 gcacatcgat ctcgatccct ttcctggcgg ccaggtccat catggtcagg gcgatcacga
     1861 cagggatttt caggtcgatg atctgggaag aaaacaggag gttccgtttc aggttattgg
     1921 catccacgag caggatgacc atttccactt ttacctcttc atcctggtta agcagcactt
     1981 tgtacgtcac ccattcgtcc gagcgcttgg ggtacaggct atacgtgccc ggaaggtcca
     2041 cgatgctggc ggaatagccg ggcgtcagat cgctggatcc ggttttcttg tcaatggtta
     2101 cgccggggaa attgcctact ttctgattca ggccggtaag ggcattaaaa agggagcttt
     2161 ttccactgtt cgggttgcca acgaggccga tatgtatttt tcttttattc aataaacctt
     2221 ttccggatta agcggaaaat gaggcaacaa aattaacctt gtaaagccgg attgatttac
     2281 gaattccgga gttaaataaa ccagcccagc agcaggcaga tgaaaacgat cacgggagaa
     2341 ggcaggcggc tgtacaaaag caggaggaaa gtgatgaagg tcacgaagac ctccagcccc
     2401 aggatcaggt tattggaaaa ggaaaagtcc ttggtcatat acaggctgcc ggcgaccata
     2461 atgcccacca cgacggcatt gatcccttcc agcgcgcggt agacggcggc atattttttc
     2521 aggttcgacc agatcgggaa aaagaaaagc accagcagcg cgctgggtaa gaaaatggcg
     2581 atcgagccga tgatacagcc cagcacctgc atcgggatgc ccatatcctt catcgcgatg
     2641 ccgcccatat aggaagaaac ggagaagacc ggtccgggta tggcccgcac cattccggcc
     2701 ccggtgtaaa attcatcttt gccgatcttg acgatattgg gattgcgctc gatcaatttc
     2761 tctgattcgg gcctggccac aaactgttcg tacatcaggg gaatgagaac ctgtccgccc
     2821 ccgaatacca ggctcccgaa ccggtacgtg ttttcaaaca ggttgatcgg cctccggtgc
     2881 ggccagttat tttttctcgc catttcactc gtgataccgg ccaggataaa tatgatggcg
     2941 aagagccaga tattggccca tttgattttg cgggtgagca cttccacctg gggaatacgc
     3001 ttgctgctca ggttggtgat aaagccgccc agcacgatca ggatcggaaa gatccagggc
     3061 gttttgaaca ggaaaaaggt ggccacggta gccaccagca ggatgcaatg ggtaatggta
     3121 ttgtgaaccg atatcttgaa ggctttccag gaggcataga tcaaaaatcc cacggtcatg
     3181 ggctggatga atttgaacag ttcggcatga ctgcctttct ggctcatgaa ttcaatgaaa
     3241 aaggaaaaaa aacccatgag gatgcaggcc ggcgtgatcc agatgagcag ggtaagaaca
     3301 gccaggggga tgccacctct tttataaccg atcagggtga gggtctgcgt ggaggaagcg
     3361 cccggaagta actggcagaa cgcattatag tccatcagtt cttcctcggt gatatcctta
     3421 cggccgttca caaaggtctt catcatcatt cccaaatgac cctgcgggcc gccgaaagcg
     3481 gaaatgctgt ataaaagaac agctttcaga aaccgcatat gacggagcag caccatggaa
     3541 tattttcagc cggcagaaaa ttccgcggga caatttagag attgttattc aatttaaaat
     3601 ttatgagggg aaccgttgac cgattgcagg agcgaatcca cggaaaagtt tacctgttta
     3661 ttatacgggc cctttttaaa gaatcttctc ccttgaatat actttcctta tggatccccg
     3721 ggttggactg gttgaactgg aattcctggt cagccagctt gatataatac aggaaatcgt
     3781 aatcggtggc gcttttggca aagaggtaag aaggccggta ggccagcata aaagaatcca
     3841 gcaggtctcc gtccagcccg gtgatccttt ttatgaggga gcggttgaac cggtggtcca
     3901 caaatttatc ctgttcatct tcctccagcc tttcccggaa ggccagggag cggcggatct
     3961 gacggaactt gagcatattg atcagcgcat ccaggtctac tcccactccc agcccgccgc
     4021 tggaaggatc ggtcagctta aagcccggtt ttctgtaatt gaaggatttc tcataatcga
     4081 tccggttttg cagggagtcc atatgatagt cgcggggggc gacccgcacc tcactcagtt
     4141 cggtcacgtt aacatggagg gcaatgtcga aaccggtggt atggttcatc tcgctgacgg
     4201 caaacatctg ggtggctctt ccgaggtaag aaaaactcag ggaatccttt tcatttacac
     4261 ggatggaata attcccgttg gcatccgtgg tggttccccg gccggtattg gatatcacgc
     4321 tcacggcttc gagcggatgc gtccggtaca tattgaagac cgttccccgg atcgtcactt
     4381 cctgtgcgcc ggcctgcatg gccaaaaaaa caagcaatcc cggcaaaatc catcttctaa
     4441 aatgtgcgta gaaaaagacc aatcaggtga ttttaaggtg attcggtggc aaaatagtaa
     4501 gaagcccccg gaaaattatg ggtttaacga taatttggat tccatccagc gggcgatctg
     4561 ttccgggtaa tgggcatgtt ccagggcatg gatcttttga gccagcgttt ccgccgtgtc
     4621 ttcggcagaa acggggcagc gggcctggaa aagaacctgt ccgtggtcat attgttcatc
     4681 cactttgtga atactgatgc cgctttcttt ttctccggcg ctgatcacgg cctggtgtac
     4741 cgcatttcca tacattcctt tcccgccgta tgccgggagc agggccggat gaatattgat
     4801 gatatggtcg gggtaagccc ggatcagctc cgggggtatc ttccataaaa accctgccag
     4861 cacgaggaag cggatatttt ttttctccag ttcgcccaga taatgcgcgc cgcggtaaaa
     4921 ttcttcccgc tcgatcagga gggagggaat gttttctttt tgtgcgattt ccagaacgcc
     4981 ggcgcccggt ttattgcaga ggatgaggga aacccggacg cggtgtccgg aaaccgcatg
     5041 ttcttcctga tgaaaatacc ggattatttc ccgggcgttg ctgcccctgc ccgaagcaaa
     5101 gagggcgatg ctgatctgct tcccgttatc tttcattgat cccgctcggt ttttttttaa
     5161 tgaggaaaga aggcttccgg ccatccgtct ttcggtaaag atgagtgaaa aatctggttt
     5221 gccggcccgc tttttcaaac caaaaattca ttgaaatttc aatgacaaat gaatgacaat
     5281 tatttttcga aaatttatat aatctgctga aacgcaagcc agatcaggga aaaaaaattt
     5341 atggcatgtt cgtattttgt taatttcatg gcttactttt gcaaccgatc caagtgattt
     5401 ttcacattta tacaacgaat tgtatatatg cattattcca gactcagtgc attcagaata
     5461 tcctatagcg tcctcctcac ctctgtaatt ctgctttttg ctagtgtatc gatggctcag
     5521 gacgggaagg ctttatttca gagtaattgt gcatcctgcc acaatcccat taaagtagtc
     5581 acgggtcccg ccctgaaagg ggtcacgagc cgggttcccg atcagaaatt attacatgcc
     5641 tggatccata ataatacggc ggtcctcgct tccggtaata aatacttcaa cgacctctac
     5701 gtccagttca gcaagacccc gatgaatacg tttccgggct tgagcgatgc ggaaattgac
     5761 gccatcctga aatatgttga atcctatcct gaaccgggaa agaccggccc ttccccgacc
     5821 gggggagaac aggcggtgcc gctgaacgac aacagcctgc tgtacggcat cctcaccctg
     5881 atcctggcgg tgatcatgct tgtgctgatg caggtgaaca gcaatcttaa aaaactttcc
     5941 gacgataagt ccggcatccc cgccggagaa ccgatcccct tttataagaa caaggcctat
     6001 atcgccatga tcgtgctggt cctttttgta ctgggcggtt atttcctggt gcaggcagcc
     6061 atcggtttgg gcaggcagca gaactacgag cccgttcagc cgatcttctt ctcccataag
     6121 gtgcacccgg ggaataacca gatcagttgc ctgtattgtc atggaggcgc ctgggacagc
     6181 aaacatgcgg gcgttccctc gctgaacgta tgtatgaact gtcatgcagc catcaacgat
     6241 tatcatggcg aacccctgca ccgcgcagac ggcagcaccg tggatgcgaa cgcggaaatc
     6301 aaaaaattat acagctacac cggttatgat ccggcttccg gaaaatatac cagccccggc
     6361 aaacagattg aatgggtgaa aatccacaat ctgcccgatc acgtttactt caaccactcc
     6421 cagcatatcc gggcgggcaa ggttcaatgc cagacctgtc atggaaatat caccgagatg
     6481 aacgaggtta aacagtttgc cgagctgagc atgggctggt gcatcaactg tcatcgtacc
     6541 acgaaagtca atttccccga cggtaaaggg aacgggaata aattctatag catttaccaa
     6601 aagttccacc aggacctcaa gtccggtaaa atggatagcg taacggtgga gaatatcggg
     6661 ggcacggaat gtcagaagtg ccattattaa tgtgctaatg ttgaaatatg aaaacaggaa
     6721 aatgggctat ggtcattttc gggttttcag attttcagat tttcaaattt gaatctaaat
     6781 gagcgaaaaa aagtactggc agaattttgg agaatacagt agctccgaag cttcccggaa
     6841 aatgagcgaa aatgaattcc aggaagagct tccgatggag gggttcgaca gcaagggcct
     6901 gctggatgtg aaaacgccgc gcagggattt tctgaaatac ctcggctaca gtacggccgc
     6961 agccaccctg gcagccagtt gcaagatccc cgttagaaaa gtggttccct atctgaataa
     7021 gcccgataac atgattccgg gtgttgccga ttattatgcc agcacttatg tttccggtgg
     7081 agacgtgact ccgatcgtgg cgaaagtgcg cgacggacgt ccgatcaaac tggaaggaaa
     7141 tgaattatcc ggcctcacca aaggcggaac ttccgcgcgg gtccaggctt ccgtgctgga
     7201 tctctatgat acggcccgtt tgcgttaccc ggtgcagatg ataaaagggt taccgcagga
     7261 agtaacgagt tttgaagctt tcgataaact ggtttcggat tccatggcgg gccagaccgg
     7321 ctccatcgtg ttgctgacca gcaccatcac ctctccgacc agtaaccagg tgatcaacga
     7381 attcctggcg aaatatccgg gcagccgcca cgtacaatac gacaccgatt cgttcagcgg
     7441 aatgctgctg gccaatgaag cggcttacgg aaaacgcagc atcccttctt accggttcga
     7501 taatgcaaaa gtaatcgtga gcctgggcgc cgattttctc gggacctggc tttcaccggt
     7561 tgaatacgcc cggcagtatg ccacgggcag aaagatcaat gagaaggcgc cggaaatgaa
     7621 ccggcattac cagttcgaat ccctgctgag ccttacgggc gccaatgccg atcagcggta
     7681 tgcccataag ccttccgaaa ccggcgcagt cgcgctggcc ctgctggcgg ccctcggcgg
     7741 aaatgttacc gctcccgcac tctccgataa actgaaagcc ggaattgcca aagcagcaaa
     7801 agacctgacc gcagcaaaag gaaaggcgct tgtcgtttcc ggaagtaatg atgtaaatgt
     7861 tcagctcatt gtaaatgcca tcaacgaaac ggtgggggcc aatgggacca cgataaactg
     7921 ggcggctccg ctcctgaccc gccagggaat ggacagtgaa tttgcgacgc tcgtaagcga
     7981 tctgaatgaa ggcaaggtcg gtaccctgct gatttacgat gtgaacccgg cctatgatta
     8041 ttatgatgcg aagaaattca ccgacggcct gcagaaagtg aagaccagcg tctccttcaa
     8101 cggccgcctg gatgaaacca ccgagctgtg caagttcgtt cttcccagtc cgcacttcct
     8161 ggaaagatgg ggggatgcgc aggccaagcc cggttatatc tccatgatcc agccggtgat
     8221 tgctccgctc ttcctgaccc ggccttacga agaatccctg atgaagtgga gcggcaacaa
     8281 ccagacttac gatgaattct ttaaacaata ctgggaagga aagctcggcg gactggaagc
     8341 ttatgaaaaa gcgcttcagg acggagtgat cgaacccact gaaatgcccg ctgctgccgg
     8401 cggttcttat aatggggcaa aactcgcgga tgccgcttct gccatcagct cttcgaaaaa
     8461 aggcagcgct ctcgaactag tcatttacca gcaggtgagc atgggggtcg gttcacagcc
     8521 caacaacccc tggctgctgg aattgccgga tcccattgcc cggtccacct gggacaactt
     8581 cgcgatggtt tcgcccaaat tcctgataga caattatgat attgacctgg gcaaccagca
     8641 tcaggcagat aaatttgaag tgcacccgga acgtccggtc attaaaataa aggcgaacgg
     8701 aaaagaaacg accattccgg tcgtcgccgt tcccggggtg gccgacgggg ttatcgccat
     8761 cgccgtggga tatggtcacc agagtgccga tccgaaaaat acggccagct ttatcggccg
     8821 ggcagttccc ggtgcaggcc ggaatgccta tccgtttgcg agcttcaacg gaacaacggt
     8881 cgactggcag gccacggatg taagttttga aaagaccaac gaagaatacc agatcgcgtt
     8941 cacccagacg cataattcct acgaaggccg tacctcggtg atcaaggaaa tgaacctgga
     9001 agaatttgtg aagaatccca atgaggtatt ggaagaaagg gagaatgagc tgaaacattt
     9061 cggagggatt gaaaattttg agaaagaggg aaccctgtac ggggtctacg ataaacccgg
     9121 cattcactgg ggcatgtcca ttgacctgaa ttcctgtatc ggctgcggcg cctgcgtggt
     9181 ggcctgtaat gcggagaata atataccgat ggtcggaaag aatgaagtga tgcgctttca
     9241 tgacatgcag tggatgcgta tcgaccggta ttttaccggc gattattcgg atccgcacag
     9301 cgtgcaaacg atcttccagc ccatgctttg ccagcattgc aataatgcgc cctgcgaaaa
     9361 tgtttgcccg gtttccgcca ccaatcacag cagtgaaggc ctgaaccaga tgatatacaa
     9421 ccgctgcatc ggcacaaaat att
//
LOCUS       DASYCU010000349         3090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4281537, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000349 DASYCU010000000
VERSION     DASYCU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4281537"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1034)
                     /locus_tag="VGM24_07115"
     CDS             complement(123..1034)
                     /locus_tag="VGM24_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155178.1"
                     /translation="MKIPLLLVQFLYKNRRLALPGIGIFTLDQSIVLPEENDTALLSL
                     PNGVQFQNANIAAADKELITYICENTGKIRPLAISDLESFLTLGMEMLNIGKPFHLEG
                     IGTITKKGGKLDFVPGEYALIKEGHPGTAPDHSKPKRAEPEQPSSAFRSSQNQNLLRG
                     LAILAGLVIIGWGGYLLYKKTAVPGAENKTEAVVPQQSPAAAADSTTPAGKGDSEKIN
                     KPAAQHAAGNKDSVVYKFIILPTNNKAHALRRYRQLLSFDLRAHLYEKDSSFFKVYFQ
                     FPALSKDTVHIKDSLKRQYAHEVTIEE"
     gene            1203..1535
                     /locus_tag="VGM24_07120"
     CDS             1203..1535
                     /locus_tag="VGM24_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155179.1"
                     /translation="MLTEEEESFIKYWEANRERQKKTFRQFLLGIPVALLFVIPIVIN
                     FFSGWYKRAAMIVHTGSFNPGVLLLALLLITVFIALFSRKFRWDQQEQRYLELLAKKK
                     KQASAEKK"
     gene            1684..>3090
                     /locus_tag="VGM24_07125"
     CDS             1684..>3090
                     /locus_tag="VGM24_07125"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY4155180.1"
                     /translation="MPYEPISVFDIFKIGVGPSSSHTLGPWRASQSFVRLLEARGLLG
                     DLKTIRIQLFGSLAKTGKGHGTDIAVQLGLSGYDPILIDTASISGILEGIRMSKNLQP
                     AAGINIAFDPQADIEFRVKESLPHHPNALRFLAGFNHSAPVEETYYSIGGGFIETGER
                     MNAARSGPSLPFPVDSAAELLHWCMKTGFTIHEVVLENERVWRDESLIKKELLEIWRV
                     MKECIYTGCHTAGILPGGLNVSRRAAALNKKLLGGRPDNDPEEWTRSIRDGGKDFNYI
                     LDWVSCFALAVNEVNASFGKVVTAPTNGAAGVIPAVLQYFVCFCDATEEQIISFLLTA
                     GETGSIFKKGATLSAAMGGCQAEIGVSSAMASAALTEGMGGTQRQSLMAAEIAMEHHL
                     GLTCDPIGGLVQIPCIERNTMGAIKAIMASQLALQGKPDFAKVSLDAVIRTMWNTALD
                     MNSKYKETSDGGLAVQIPI"
BASE COUNT          745 a          692 c          805 g          848 t
ORIGIN      
        1 cattcagggc gagattcagg gttagaagag ataaagataa aacgaagatt tttttggaag
       61 aaagcgaatc ggtgatatgc atgttcaatc ttttaaattg aattgaagtt agcataaatt
      121 ttttattcct caatagtcac ctcgtgtgcg tattggcgtt tgagcgaatc cttgatgtga
      181 acggtatctt ttgataatgc cggaaactgg aaatagactt taaaaaagct ggagtctttt
      241 tcatacaggt gagcccggag atcaaacgaa agaagttgcc ggtatcttct cagggcatgc
      301 gccttattgt tggtgggaag gataataaat ttgtaaacta ccgagtcttt atttcccgcg
      361 gcatgttgcg cagccggttt gttgatcttt tcggaatcgc ctttccccgc tggggttgtg
      421 ctatcggctg ccgctgcagg agattgctgc ggaacaaccg cttccgtttt attttccgct
      481 cccggcaccg ctgttttttt atacaggagg tatccgcccc atccgatgat gaccaggccg
      541 gcaagaattg caaggcccct gagcagattt tggttttgcg aagaccggaa cgcggatgaa
      601 ggctgttcgg gttcggccct tttgggtttg ctgtggtccg gcgctgttcc cggatgacct
      661 tctttgatca gcgcatattc tcccggaaca aaatccagtt tgcccccttt tttagtgatg
      721 gttccaattc cttccaggtg gaacggcttg ccgatattta gcatttccat tcccagcgtc
      781 aggaaagatt ccagatcaga aatggcgagc ggcctgattt taccggtatt ttcgcagata
      841 taagtgatca gttctttgtc ggctgcagcg atatttgcat tttgaaactg aactccgttt
      901 ggtaaggaca gcagggccgt gtcattttct tccggaagaa cgatggactg gtcgagggta
      961 aaaatgccta tccccggcaa ggcgagcctt ctgtttttgt ataaaaactg tacaagaagt
     1021 aatgggatct tcaaagccaa tggatttcgg attttaaatg taaagaatcg aacgctaatt
     1081 agcagtaata attatatgaa aattaaaata aaatattgaa aactagcatt ctaactacat
     1141 aaacttcgat ttttgttcaa tatcggatga tgccataata tccggtcaaa tcgtataaag
     1201 tcatgcttac ggaagaagaa gagtccttta ttaagtactg ggaagccaac cgggaacgcc
     1261 agaaaaagac tttccggcaa tttttgctgg gtattccggt agcccttttg tttgttatcc
     1321 cgatcgtgat caatttcttt tccggttggt acaagagggc ggccatgata gtccatacgg
     1381 gcagcttcaa cccgggtgtt ctgctcctgg cgctgctcct gatcaccgtg tttatcgccc
     1441 ttttctcgcg taaatttcgc tgggaccagc aggagcagcg ttacctggaa ctcctggcaa
     1501 aaaagaaaaa acaggcttcc gctgaaaaaa aataatttct tgcagcgttt taaagtaggg
     1561 ctttgtctta ttaaagcata tcaagtaatt gttttgcgtg ccgtaaccat agactacctg
     1621 caagactaac cctggacagc ctgttcgggg ttttttattg tatttttggt caaatctatc
     1681 tttttgccct acgaacccat atcggttttt gatatattca aaataggggt cggaccttcc
     1741 agttcccata ctctgggtcc ctggcgggct tcgcagtcct ttgtccgttt attggaagcc
     1801 cgggggttgt tgggggatct gaaaaccata cggatccagc tttttggatc cctggcaaaa
     1861 acgggaaagg gccatggaac cgatatagcg gttcagctgg ggttatcggg gtatgacccg
     1921 atccttatcg atacggcctc tatttccggg atcctggaag gcatccggat gagtaaaaac
     1981 ctgcagccgg ctgcaggaat aaatattgct tttgatccgc aggcggatat tgagttccgg
     2041 gtgaaggaat ctctccccca ccatcccaat gccctccggt ttcttgccgg attcaaccat
     2101 tctgctccgg tggaggaaac gtattattcc atcgggggtg gatttattga aaccggggaa
     2161 aggatgaatg ccgccagatc cggtcccagc cttccctttc cggtggattc agcggccgaa
     2221 ctgctgcatt ggtgcatgaa gaccggtttt accattcatg aggtcgttct ggagaatgag
     2281 cgtgtttggc gggacgaatc cctcataaaa aaggaattgc tggaaatatg gcgggtaatg
     2341 aaggaatgca tttataccgg ttgccataca gcgggtatat tgcccggagg actgaatgtc
     2401 agccgccggg cagccgcgtt aaataaaaaa ttattgggcg gaaggccaga caatgacccg
     2461 gaagagtgga cccggtccat ccgggacggc ggcaaggatt ttaattatat cctcgactgg
     2521 gtaagttgtt ttgccctcgc cgtgaatgaa gtaaatgctt ctttcggcaa agtggttacg
     2581 gcgcctacca atggggcagc cggggtgatc ccggcggtac tgcagtattt tgtttgtttt
     2641 tgtgatgcga cagaggaaca gatcatatcc tttttactta ccgccggtga aaccggaagc
     2701 attttcaaga agggggctac cctttccgct gccatggggg gatgccaggc ggagatcggc
     2761 gtatcttctg ccatggcttc cgcagccctt accgagggga tgggtggtac ccagcggcaa
     2821 tcccttatgg ccgccgagat tgcgatggaa catcatttgg gtctgacctg tgatcctatc
     2881 ggcggcctcg tgcagatccc ctgcattgaa cgcaatacga tgggagccat caaggccatc
     2941 atggcttccc agctggccct gcagggcaag ccggattttg cgaaagtatc cctggatgcg
     3001 gtgatccgga ccatgtggaa tacggccctc gatatgaaca gcaaatacaa ggaaacctcg
     3061 gatggcggtc tggctgttca gattcctatc
//
LOCUS       DASYCU010000350         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2109051, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000350 DASYCU010000000
VERSION     DASYCU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109051"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VGM24_07130"
     CDS             <1..864
                     /locus_tag="VGM24_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4155181.1"
                     /translation="RADHSFGKVSATVDLGFGTRAEEFAYNDGNTRLAVKQLYVTYSP
                     SSKIKFTLGTWGTHVGYEVLDAYLNRNYSMSYMFTYGPFSHTGLKADISLGGKSALML
                     GISNPTDYRSAPGMPKSFIAQFSTGSKDDKFKAYLNFVDGKQTEDKNLMQEDLVLTYA
                     ISGKFSLGYNGTYQTLNLKQPDNSWSSVNWWGSALYLNADPVSWFGLTLRGEYIGNTD
                     GWLTNSVGSNSYDLGNILEATLSANFRIDNLIIIPEFRLDNARNAAFYKNSTDLTKST
                     GSFILAATYHF"
     gene            complement(980..1666)
                     /gene="cmk"
                     /locus_tag="VGM24_07135"
     CDS             complement(980..1666)
                     /gene="cmk"
                     /locus_tag="VGM24_07135"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY4155182.1"
                     /translation="MAEKKIIITIDGWSSCGKSTLAKQLARKLKYTYIDSGAMYRAIT
                     LYFLRNHVDWTNPSSVAEALGDIVIEFIVNSKTGDSETWLNGENVEYLIRDLVIAEKV
                     SDVAAIASVRTFAVKQQQQLGLNSGIIMDGRDIGTVVFPKAELKIFMTADIAVRVERR
                     FKELFEKNPNISIEEVKANLELRDYIDSHRAVSPLRKAEDAIELDNTLLNPEEQLSLV
                     LKWTKERMKR"
     gene            complement(1756..4281)
                     /locus_tag="VGM24_07140"
     CDS             complement(1756..4281)
                     /locus_tag="VGM24_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY4155183.1"
                     /translation="MDNNFSAQVKEIISFSREEALRLGNDFIGTEHLLLGLIREGDNT
                     AVRILKSFNVDLYELRKEVELAVKDKTGKNIANINSLPLTKQAEKVIRVTVLEAKALK
                     SPTVETEHLMLSILKNKENIATQILNQFDVDYDLFRNELGVVKSNDTRSEYAEENDDD
                     FDDEKKYAQQKARPAGNAKSKTPVLDNFGRDITRLAEMNSLDPIVGREEEIERVSQIL
                     SRRKKNNPILIGEPGVGKTAIVEGLALRIVQRKVSRVLFDKRVISLDLAALVAGTKYR
                     GQFEERMKAIMNELEKNRDVILFIDEIHTIVGAGGASGSLDASNIFKPALARGELQCI
                     GASTLDEYRMYIEKDGALDRRFQKVMIDPPSVEETIQILVNIKSKYEDYHNVVYSNDA
                     IEACVKLSDRYMTDRLLPDKAIDVLDEVGARVHLKNINVPQNIVELEKKIEDVKNEKN
                     KVVKSQKFEEAASLRDAEKRLQEELEKAKAEWEEESKHKRYPIDEENIAEVVSMMTGI
                     PVKRMVQAESEKLRNMSVDMADMVVGQTDAIQKVVKAIQRNRVGLKDPRKPIGTFIFL
                     GPTGVGKTELARALARYMFDSEDSLVRIDMSEYMEKFTVSRLIGAPPGYVGYEEGGQL
                     TEKVRRKPYSVILLDEIEKAHPDIYNILLQVLDDGQLTDGLGRKVDFKNTLIIMTSNI
                     GARQLKDFGDGVGFATSARTANVDENNKAVIEKALKRTFSPEFLNRVDDIVIFNSLNR
                     ENIFNIIDILMKGVMKRLSQLGFSLELTVEAKSFIADKGYDQQFGARPLHRAIQKYLE
                     DPLAEEILNMNIKNGDIMIADLDKELGKIKFTLKEKEKEASEV"
     gene            4374..4742
                     /locus_tag="VGM24_07145"
     CDS             4374..4742
                     /locus_tag="VGM24_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY4155184.1"
                     /translation="MNVKISTNEKFHAITIRETELAANMTEDLRECLEPFLHNEVKNL
                     ILVMKDIQKIDSAAAKELLQIRNRFLENRASLVLCELQAPVKEIFDHNEQYESLQITP
                     TFSEAADMVYLEEIEREMTD"
     gene            4918..>5236
                     /locus_tag="VGM24_07150"
     CDS             4918..>5236
                     /locus_tag="VGM24_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4155185.1"
                     /translation="MLAVTILGNNSAIPAFDRHPTAQVITMDDQLFLIDCGEGTQMQM
                     SKYKIRRSRINHIFISHLHGDHYFGLIGLITSMGLLGRQQDLNLYAPAPLKEIFDMQL
                     KVAD"
BASE COUNT         1210 a         1422 c         1150 g         1454 t
ORIGIN      
        1 cgcgcagacc atagttttgg taaagtgagt gccacggtag acctgggttt tggaacccgg
       61 gcggaagaat ttgcttataa tgacggcaac acccggctgg cggttaagca actgtacgtt
      121 acctattccc cctcttcaaa aataaaattc actttaggaa cctggggcac ccatgtgggc
      181 tatgaagtgc tggacgccta tctgaaccgc aattacagca tgagctatat gtttacctac
      241 ggacctttct cccacaccgg gttaaaggca gatatcagcc tgggtggaaa aagcgccctc
      301 atgctgggga tctccaatcc gacggattac cgctcggcgc ccggaatgcc gaaatccttt
      361 attgcgcaat tctccaccgg atcaaaggac gataaattca aggcttatct gaatttcgtt
      421 gacgggaagc aaacggagga caagaacctg atgcaggaag acctggtgct gacctacgcg
      481 atctccggaa aattcagcct gggatacaat ggcacctacc aaaccctgaa tctgaaacag
      541 ccggacaata gctggagttc cgtgaactgg tggggatccg ctttgtacct gaatgcagac
      601 cccgtcagct ggttcggcct caccctcagg ggagaataca tcggcaatac cgacggctgg
      661 cttaccaaca gcgtaggctc caacagctat gatctcggaa atattctgga agcaacgctt
      721 tctgccaatt tccggatcga taacctgatc attatccccg agttcaggct ggataacgcc
      781 aggaatgccg ctttttataa aaacagcacc gacctgacga agtcgaccgg gagtttcatc
      841 ctggcagcca cctatcattt ttaagcccca tcagcaccgg cgcaacggat tccgggatcc
      901 gttttcagta cgaagcggct gccttccatc atccgggggc agccgcttct gtttccggaa
      961 caaacgccca tgggtgatct caacgtttca tcctctcttt tgtccatttt aatacaagcg
     1021 aaagctgttc ttcgggattt aaaagcgtat tgtccagctc aatggcatct tccgcttttc
     1081 tcaggggact taccgcccgg tgggaatcga tataatcccg cagttcgaga ttggccttta
     1141 cttcctctat actgatattg ggattctttt cgaacagttc tttaaaccgg cgctccacgc
     1201 gtacggctat atcggcggtc ataaatattt tcaattcagc cttcggaaat acgactgttc
     1261 cgatatccct tccgtccatg atgattccgc tgttcaggcc cagttgctgc tgctgtttaa
     1321 ccgcgaaggt ccgtaccgaa gcaatagccg ctacgtcact gactttttct gcgatcacca
     1381 ggtcccggat caggtactct acattctccc cgttaagcca ggtttcgcta tccccggttt
     1441 tggaattcac gatgaattcg atcacgatat ccccgagggc ttccgcaacg gaggagggat
     1501 tggtccagtc cacatgattc cgcaggaaat acaaggttat tgcccggtac atggccccgc
     1561 tgtcgatata ggtatacttc agcttccggg cgagttgttt agcgagcgtg ctctttccgc
     1621 aggacgacca gccgtcgatg gttatgatga tctttttttc cgccatgaac ttttattccc
     1681 tgcaaataaa aacaattgcc acggaaacac ggaaattttc cgcgcgccgt ggcaatgtat
     1741 attattatat gcttcttata cttcggaagc ttccttctct ttttccttca gggtgaattt
     1801 aattttcccc agctccttgt ccagatcggc gatcatgata tctccgttct tgatgttcat
     1861 attgaggatc tcttcggcga gcggatcttc caggtatttc tgaatcgccc ggtgaagcgg
     1921 tctggctcca aactgctgat catacccctt gtctgcgata aagctttttg cctcaacggt
     1981 cagctcgagg ctgaagccca gctgcgacag tcttttcata acccccttca tcaggatatc
     2041 aatgatattg aagatgtttt ccctgttcag cgaattgaag atgacgatat catccacgcg
     2101 gttcaggaac tcggggctga atgtgcgttt cagggctttt tcaatcaccg ccttattgtt
     2161 ttcatccaca ttggccgtcc gggccgaagt agcaaacccc accccgtcgc caaagtcttt
     2221 caactgcctt gccccgatat tggacgtcat gatgatcagc gtattcttaa aatccacttt
     2281 tcttcccaac ccgtcggtca gctgaccgtc gtccaaaacc tgcagcagga tattatagat
     2341 atccggatga gctttttcga tttcatcgag caggatcacg gagtagggtt tccttctcac
     2401 cttttcggta agctggcctc cttcctcata acccacgtat ccgggaggtg cgccgatcag
     2461 ccggctgacg gtgaacttct ccatgtattc gctcatgtct atccggacga gcgaatcttc
     2521 cgaatcgaac atataccggg caagggcccg ggcaagttcc gttttaccga ccccggtcgg
     2581 gcctaggaaa ataaaggtgc cgatgggctt ccgcgggtct ttcaatccga cccggttccg
     2641 ctggatggcc ttcaccactt tctggatcgc atcggtttga ccgaccacca tgtccgccat
     2701 atcgaccgac atgttccgga gcttctcgct ttccgcctgt accattcttt tcaccggaat
     2761 acccgtcatc atactcacca cttcggcaat attttcctca tcgatcggat accttttgtg
     2821 tttgctttct tcttcccatt cagcctttgc tttctcgagt tcttcctgca aacgcttttc
     2881 cgcatctctc agggaagcgg cttcttcaaa cttctgactc ttgacaacct tattcttttc
     2941 gtttttaacg tcctcgatct tcttttccag ctccacgata ttctgcggta cattgatatt
     3001 cttcagatgg accctcgccc ccacttcatc caaaacgtca atggccttgt ccggcagcag
     3061 gcggtctgtc atgtaccggt cgctcagctt tacgcaggct tctatcgcgt cgtttgaata
     3121 aacaacattg tggtaatctt cgtatttgga tttgatgtta accaggatct gaatggtttc
     3181 ctccacgctg ggcggatcga tcatcacttt ctgaaaacgg cggtcaaggg cgccgtcctt
     3241 ttcaatatac atcctgtact cgtccagggt agaagcgccg atgcactgga gttcgccccg
     3301 ggctaaagcc ggtttgaaaa tattggaggc atccagggaa ccggaggcac ctcccgcccc
     3361 aacgatggtg tgtatttcat cgatgaacag gatgacgtcc ctgttttttt ccagttcatt
     3421 catgatcgcc ttcatgcgtt cctcgaactg tccgcggtat ttggtaccgg cgaccagcgc
     3481 agccagatcc agggagatca cgcgtttgtc gaacaatacc cgggaaacct tgcgttgcac
     3541 gatacgcagc gccaggcctt ccacgatggc cgttttaccc actccgggtt cgccgatcag
     3601 gatcgggtta ttctttttcc gccgggacag tatctgcgag acccgttcga tttcttcctc
     3661 cctgcccaca atgggatcca gggaattcat ttccgccaga cgggtaatat cccttccgaa
     3721 attatcgagc accggcgttt tacttttcgc atttcctgcc ggacgcgctt tctgctgcgc
     3781 atactttttt tcgtcatcaa aatcatcatc attctcttct gcgtattcgc tgcgggtatc
     3841 attactctta acaacaccca gttcatttct gaataaatcg taatccacgt cgaattgatt
     3901 taagatttga gttgcgatgt tttccttgtt cttcagtatg gagagcataa gatgttccgt
     3961 ttccaccgtg ggactcttca acgcctttgc ctcgagcacg gtgacccgga tcactttttc
     4021 tgcctgcttg gtaagcggca ggctgttgat attggcaata ttcttacccg ttttatcttt
     4081 aaccgccagc tccacttcct tgcggagttc gtaaagatcc acattaaagc ttttcaggat
     4141 acggacggcc gtattgtcgc cttcccggat cagtcccaga agcaaatgct cggtaccgat
     4201 aaagtcgttt cccaacctta aggcttcctc cctgctgaac gaaataatct ctttaacctg
     4261 ggccgaaaaa ttattatcca tttttaagta attgtgctta atacaataat aacgaatatt
     4321 tttgattcct gttttccatg gtttatcaac tatgttaaca ataccaggtc attatgaatg
     4381 tcaaaattag taccaatgag aaattccatg ccattaccat ccgggaaacg gaacttgctg
     4441 ctaatatgac agaagatctt cgggaatgcc tggagccttt tcttcacaat gaggtcaaaa
     4501 acctcatcct cgttatgaaa gacattcaaa agatcgattc tgctgctgcc aaagaactgc
     4561 ttcagatccg gaaccgcttt ctcgaaaacc gtgcctccct ggtgctctgc gaacttcagg
     4621 cgccggtaaa agaaatattt gaccacaatg aacaatatga aagccttcaa atcacgccca
     4681 cattcagtga agcggccgac atggtttatc tggaagaaat cgaaagagaa atgactgatt
     4741 agacgaaatt tcctgcctct tttatacaat cctcatcaac atttgttcat tcaatggctt
     4801 ccggatacaa tggaaacgac cgaatcccgt tcctattact ttttgtatta tttttcctgt
     4861 atggcagcag tcccccaacc ggaaactgtc cgtttgatca taccttaaaa tctacttgtg
     4921 ctggcggtaa ctattcttgg taacaattct gctattccgg ccttcgaccg gcacccgaca
     4981 gcacaggtta ttacgatgga tgaccagctt ttcctgatcg attgcggcga aggcacgcag
     5041 atgcagatga gcaagtataa gatccgcagg agccggatca accacatctt tatttcgcac
     5101 ctccacgggg atcactactt cggtttgatc gggctcatca ccagcatggg attgctgggc
     5161 cgccagcagg acctgaacct ttatgcaccc gccccgctta aagaaatctt cgacatgcaa
     5221 ctgaaagtgg ctgata
//
LOCUS       DASYCU010000351         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4717148, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000351 DASYCU010000000
VERSION     DASYCU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4717148"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VGM24_07155"
     CDS             complement(<1..1004)
                     /locus_tag="VGM24_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY4155186.1"
                     /translation="MAETTTPRLMAAAKEFNIGRDTLIDFLAGKGFNKEDLKPTAKLT
                     EEMYHSLQQEFQGDKVAKIKSNQIDLPKGSVEAKRKKEDEAVLFRKDPKKASREEPVA
                     PVVEEPKVVKAEEPKKPKAEEPKKEPEITKIEAPDIEGPKIIDKIDLSSIDSSTRPKK
                     TSKKKTETAAQKEEPVVAEDKVGETEPGVPAETEKAEEANTIENIRAEKIEGPRILGK
                     IELPVEADKPKQLDEKRKRKRIPIEKRDTGRPATSPGTPVQRESRPGGAGRPILRRDQ
                     RGPAGGNRGNTTPREQKEIDKKEIQEKIRETQAKLAGTGGRGKSLKAKYRRARREENA
                     "
     gene            complement(1111..2358)
                     /gene="nusA"
                     /locus_tag="VGM24_07160"
     CDS             complement(1111..2358)
                     /gene="nusA"
                     /locus_tag="VGM24_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY4155187.1"
                     /translation="MASINLIEAFQDFKEAENIDRPTMMKVVEDVFKTLLRKKYGSDE
                     NFDVIVNAEKGDLEIMRRRTIVENGSVEDPLAQIAYNEAVKIEPDYEVGEELYEEIEI
                     LDFGRRAILAAKQTLASRIGDLKKNVLVKKYGERIGEIISAEVYQVWKKEILLLDEEG
                     NELILPKSEQIPQDYFKKGESIRSVVKKVDMKNNSPVIILSRTSPSFLSKLLEIEVPE
                     IFDGLIVIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INWTNNTQLLLQRSLTPSKITSMDIDSEKLHANVYLKPDQVSLAIGRKGVNIKLAQEL
                     TGYSIDVFRDNEGETTEFDIDLDEFSDEIETWVIDELKRIGCDTAHSVLDLTVEELER
                     RTDLEKETIEDVRRVLKEEFDKE"
     gene            complement(2382..2933)
                     /locus_tag="VGM24_07165"
     CDS             complement(2382..2933)
                     /locus_tag="VGM24_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HEY4155188.1"
                     /translation="MHPERWNNRKGTQSFPSLFRMHNETLIETVTAMVQEILTADPAY
                     FLVDCRIKPINNLKIFLDGDQGITIEKCVQVNRALYKKLEESGLFPGGDFSLEVSSAG
                     LDEPLKSWRQYKKNIGRLVEILLLDGSRKEGKLAEVAEDGIVLEEATGRGSAGKAVPK
                     KQTTSYHTYLFNNIKTTKIQVVF"
     gene            3034..3282
                     /locus_tag="VGM24_07170"
     CDS             3034..3282
                     /locus_tag="VGM24_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155189.1"
                     /translation="MNVFFLAILLYLLYRFIVGLVIPIFRTTRHVRQQFQQMNGQGPG
                     QAPGQPDAGGPQTRTKTRDAAHPDTSKVGEYIDFEEVK"
     gene            complement(3309..>3854)
                     /locus_tag="VGM24_07175"
     CDS             complement(3309..>3854)
                     /locus_tag="VGM24_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY4155190.1"
                     /translation="QKAFTEMGVKNINDYFGHGFAASFFKDHEAGKISDAAFIQKIRE
                     EVRMELSDEAVIGAWNALLLRFPPERIALLDEIRKKYRIFLYSNTNALHHQRFMEIYR
                     SAFPGRSLDDHFEKAYYSHVLGHRKPDSGGFEIIVRENGLDPVETLFVDDALINIEGA
                     VRTGLQGLYLPPAMGIKDIRW"
BASE COUNT          741 a         1056 c          874 g         1183 t
ORIGIN      
        1 tctgcatttt cttcccttct ggcgcggcgg tattttgctt tcaggctctt tcccctgccg
       61 ccggtacccg ccagcttggc ctgggtttcc ctgatctttt cctgaatttc tttcttgtcg
      121 atttctttct gctcgcgtgg cgtggtattg ccccggtttc cgccggcagg gccccgctgg
      181 tcgcgccgga gaattggtct tcccgctccg ccaggcctgc tttcgcgctg caccggcgtt
      241 cccggagaag tggcgggacg acccgtatcg cgcttttcaa tcggaatgcg ttttcttttt
      301 cttttctcgt cgagctgttt gggtttgtcg gcttctacgg gcaattctat tttacccaga
      361 atgcgggggc cttcgatttt ctctgcgcgg atattctcaa tggtatttgc ctcttccgcc
      421 ttttcggttt cggcgggcac acccggttcc gtttccccga ctttatcttc cgcaacaacg
      481 ggctcttctt tctgagcagc cgtttccgtc tttttcttgc tggtcttttt cggtcttgtg
      541 gaagagtcta tactggacag gtcaatcttg tcaatgattt tcggcccctc gatatccgga
      601 gcttcgatct tggtgatttc cggctctttt ttgggttctt ccgcctttgg ttttttgggc
      661 tcctcggcct tgacgacttt tggttcttcc accaccggcg ccacgggttc ttccctggac
      721 gcttttttcg gatccttgcg gaacaacaca gcctcgtcct ccttcttcct cttggcttcc
      781 acactgccct tggggagatc gatctgattg ctcttgatct tcgccacttt gtccccctga
      841 aattcctgct gaagggaatg atacatctct tccgtcagct tggcggtggg tttcaggtct
      901 tctttgttaa atcctttgcc agccaggaaa tcaatcagcg tgtctctgcc gatgttgaat
      961 tccttggcgg ctgccattag cctgggtgtt gtcgtttctg ccatattaaa ttaatccatg
     1021 aacagccggt ccgttcatgg accgtagtca cgcggtgata aatgttttat aataatgaaa
     1081 acccataatc atatagccat gatatacagg ttattctttg tcgaattctt ctttcagtac
     1141 ccggcgcacg tcttcaatgg tttctttctc caggtccgtt ctgcgctcca gttcttccac
     1201 cgtcaggtcc agcacactgt gcgccgtatc gcaaccgatg cgtttcagtt catcaataac
     1261 ccaggtttcg atttcatcgc tgaattcatc cagatcgata tcaaattctg tggtttcccc
     1321 ttcattatcg cggaacacgt cgatcgaata gcctgtcagc tcctgagcca gtttaatgtt
     1381 tacgcccttc cggccgatgg ccagggaaac ctggtccggc tttagataga catttgcatg
     1441 cagcttttcg ctgtctatgt ccatgctggt tattttggat ggggtaagcg aacgctggag
     1501 gagcagctgg gtgttgttgg tccagttgat cacgtcgatg ttttcgttct tcagttcccg
     1561 cacgatgccg tgaatacggc ttcccttcat ccccacacag gcacccaccg gatcgatccg
     1621 gtcgtcgtag gattccaccg ccactttggc cctttctccc ggttcacgga cgatcttttt
     1681 gattacgatc agtccgtcaa agatttccgg aacttctatt tccagcagct tggacagaaa
     1741 agagggactt gttctggaaa gaatgatcac cggtgaattg tttttcatat ccactttctt
     1801 cacgaccgag cggatgcttt cgcctttctt gaaatagtcc tgcggtatct gttctgattt
     1861 aggtaaaatg agttcattgc cttcttcgtc cagcagcagg atctcctttt tccaaacctg
     1921 gtatacttcc gcactgatga tctcgccgat gcgttcgccg tatttcttga ccagcacatt
     1981 ctttttcaga tccccgatcc ggctggccag ggtttgcttg gcagccagga ttgccctgcg
     2041 gccaaaatcc agtatctcaa tctcttcgta gagttcttcc cccacctcgt aatccggttc
     2101 tattttcaca gcttcgttgt aagcgatctg ggcaagcgga tcttccacag agccgttctc
     2161 cacaatcgtt cgcctgcgca tgatttcaag gtcgcctttt tcggcattca caattacgtc
     2221 aaaattctca tcactaccgt attttttacg gagcaatgtt ttgaaaacgt cttccaccac
     2281 tttcatcatg gtgggacggt caatattctc ggcctccttg aaatcctgga aagcttcgat
     2341 cagattgata cttgccataa cggatcttgt ttacatccag tttaaaaaac cacctggatt
     2401 tttgttgttt ttatgttgtt gaataaatac gtatgataac tcgtggtctg ttttttgggg
     2461 accgccttgc cggctgatcc tctgcctgtc gcctcttcca gcacaatccc gtcttcggcc
     2521 acttcggcga gctttccttc cttgcggctt ccgtccagca gcaggatttc caccagacgg
     2581 ccgatgtttt tcttgtactg tctccaggat ttcaaaggct cgtccaggcc ggcggaggaa
     2641 acctccaggg aaaaatcgcc tccgggaaac agaccggatt cttccagctt cttatacagg
     2701 gcccggttca cctgaacgca tttctcaatg gttattccct gatcaccgtc cagaaagatc
     2761 ttcaaattgt tgatcggctt tatgcggcaa tcaaccagaa aataggcagg atctgcagtc
     2821 agtatttcct gaaccatagc ggtgaccgtc tctatcagcg tctcattgtg catacgaaat
     2881 aaagaaggga acgattgcgt tcccttcctg ttgttccacc tttccggatg caaatttaac
     2941 gcatttaatt tattctttcc aaaaatgccg gtttaaattg cgattgcctt tttaacaggc
     3001 tttttgggtg ttctgtatta attttgaatc gctatgaatg tatttttttt agccattctc
     3061 ctttatctgc tttaccgctt tattgtgggc ttggttattc cgatcttccg gactacccgt
     3121 catgtccgcc agcagtttca acagatgaac ggacaggggc ccggacaggc tcccggtcag
     3181 ccggatgcgg gcggtcccca gacccggaca aagacccgcg atgctgctca tcccgacaca
     3241 tccaaagtcg gtgaatatat tgattttgaa gaagttaagt aggttctagg cttcccttcc
     3301 cgcaggaatc accagcgaat atccttgatg cccatagccg gaggaaggta aagtccctga
     3361 agtccggtcc gcacggctcc ttcgatattg atcagcgcat cgtccacaaa aagcgtttct
     3421 accgggtcga gcccgttttc ccggacgatg atctcaaatc ccccgctatc cggcttccgg
     3481 tgacccagca catgggaata gtaggccttt tcaaaatgat cgtcgaggct gcggccggga
     3541 aaagcggagc ggtagatttc catgaaccgc tgatgatgga gcgcgttggt attgctgtaa
     3601 agaaatatcc tgtacttttt cctgatctca tccagcaggg caatccgttc gggcggaaag
     3661 cgcaacagga gcgcgttcca ggctccgatc acagcttcgt ccgacaattc catccgcacc
     3721 tcctccctga ttttttgaat aaaggccgca tcgctgattt ttcccgcctc gtgatcttta
     3781 aaaaaggatg ctgcaaaccc gtgcccgaaa tagtcattga tgtttttcac acccatttcg
     3841 gtaaaggcct tctg
//
LOCUS       DASYCU010000352         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_77887, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000352 DASYCU010000000
VERSION     DASYCU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77887"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1005
                     /locus_tag="VGM24_07180"
     CDS             106..1005
                     /locus_tag="VGM24_07180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4155191.1"
                     /translation="MDAAPFSSPQCPVCKSEQVCFALKAKDHTVSGEWFEIWECHHCL
                     LRFTRNAPDAENIGRYYHSENYISHSNTRKGWLNSLYHRVRNHTLDSKYKLIRSVTGL
                     NRGEHLDIGAGTGAFVQFMNGKGWRSQGIEPDEAARRVASSDHRTSLLPAEAFQTLPL
                     SSFDAISLWHVLEHVHDLHPYLEQIKKLLKPSGAVFIAVPNYTSYDGMKYKENWAAYD
                     VPRHLYHFSPTSMKYLLDAGGFKLMRMEPMWFDSFYISLLSEKYATGHASLPGGFLSG
                     AVSNAKALLKKEHCSSLIYVAGK"
     gene            complement(1027..>1919)
                     /locus_tag="VGM24_07185"
     CDS             complement(1027..>1919)
                     /locus_tag="VGM24_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEY4155192.1"
                     /translation="FLFSRILGIYPLFLRTINFMPKILMPLLLILTLAIACKDSSRSS
                     GGSDSIDEPATDNEPAGISYTVLNATPHDTNSYTEGLLFHNGKLYESSGAPQDDSNTR
                     SIFGEVDPATGKIAVKAELDRKIYFGEGITILNGKIYQLTWKNKTGFIYDAKTFKRTG
                     QFSFPTDEGWGMTTDGTDLIMSDGTSNLTFLDPNTFKTVKIVAVKDNNGPVGNINELE
                     YIKGNIYANVYQTANIVRIDPSSGNVTGKADFSRLYKEAQMKYPATEYMNGIAFDSTK
                     NIVYITGKLWPSIYEVRLNN"
BASE COUNT          474 a          473 c          467 g          505 t
ORIGIN      
        1 atttttcatc caacggatgg agcgtggagt ccacgggaaa ccagcaggcc atccagttga
       61 gtaaataatt tttatctttc tgccgaatca attcttatca agccgatgga tgctgctcca
      121 ttttcttctc cccaatgtcc cgtatgtaaa agcgagcagg tctgttttgc gctgaaagca
      181 aaagaccata cggtttccgg cgaatggttc gagatctggg aatgccatca ttgcctgctg
      241 cggttcaccc ggaatgcacc cgatgcggaa aatatcggtc gttattatca ttcggagaat
      301 tatatttccc atagcaatac ccgcaaaggt tggctgaaca gtctgtacca ccgggtaaga
      361 aatcataccc tcgactccaa atacaaactg atccggtcgg tgaccggact aaaccgcgga
      421 gaacacctgg atatcggcgc cggcaccggt gcgtttgttc agttcatgaa tggaaaggga
      481 tggcggagcc agggaatcga gcccgatgag gcggcccgcc gggtggcatc cagcgatcac
      541 cggaccagcc tgcttccggc ggaagccttt caaacccttc cgctttcctc cttcgacgcc
      601 atcagcctct ggcatgtatt ggaacacgtg cacgacctgc atccttacct ggagcagata
      661 aaaaaattgc tgaagccctc cggagccgtg tttattgccg tcccgaacta taccagttac
      721 gatggaatga aatataagga aaactgggcc gcttacgatg tgccgcgaca cctctaccat
      781 ttttcaccca cttccatgaa gtacctttta gacgccggcg gatttaaatt gatgcggatg
      841 gaaccaatgt ggtttgacag tttttatatc agcctgctga gtgaaaaata tgctacaggg
      901 catgcttctt tacccggtgg atttttatcc ggtgctgttt ccaatgcgaa agcccttttg
      961 aaaaaagaac actgcagttc gctgatttat gtagcgggaa aatgaaggga ggggccgtgc
     1021 gcctgcttaa ttattcagcc ggacctcata aatactcggc cagagcttgc cggtaatata
     1081 aactatgttt tttgtggaat cgaaggcaat cccgttcata tattccgtcg cggggtattt
     1141 catctgagcc tctttataca gcctgctgaa atcggccttg cccgttacgt ttccgctgga
     1201 cgggtctatg cggacaatat tcgcggtctg ataaacattg gcataaatat tacccttaat
     1261 atattccagt tcattgatat tccctacggg accgttattg tcttttacag caacgatctt
     1321 aaccgtttta aatgtattcg gatcaagaaa ggtcaggttg ctcgtgccgt cactcatgat
     1381 caggtcggta ccgtcggtgg tcatgcccca gccttcgtcg gtcggaaaac tgaactggcc
     1441 ggtcctctta aaggttttcg catcgtaaat aaaaccggtt ttatttttcc aggtgagctg
     1501 gtagatcttc ccgttcagaa tcgttatgcc ctccccaaaa tagatcttcc ggtcaagctc
     1561 cgcctttacc gcaatttttc cggttgccgg atccacttcc ccgaaaatgg accgggtatt
     1621 ggaatcatcc tgcggtgcgc cggagctttc atataatttc ccgttatgaa acaacagacc
     1681 ttccgtgtag gaattggtat cgtgcggagt ggcattcagc acggtatagg aaatgccggc
     1741 gggttcattg tccgtcgcgg gctcatcgat agagtcggag cctccggagg accgggatga
     1801 atctttacag gcgatcgcca gcgtaaggat cagcaatagg ggcattaaga tcttgggcat
     1861 aaagttgatc gtccgcaaaa atagcgggta aattcctaaa attctgctaa ataaaaaag
//
LOCUS       DASYCU010000353         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2251832, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000353 DASYCU010000000
VERSION     DASYCU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251832"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..>1513
                     /locus_tag="VGM24_07190"
     CDS             35..>1513
                     /locus_tag="VGM24_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155193.1"
                     /translation="MADLCRAQAVLSEQQDRWVITPDSSIEWKIAGRLPHHDHIEMSG
                     EKVSLWVQYGVDSSGLPEVSRTVVFPTFRTKPNDTHASLMYTFTDADLPRIYINAEPL
                     RADIINGRKRGNFPRQVESIRQKGIMRIESNIGAPSFLHLEHSLFPSVDKALALETFV
                     FTNTSAKQASVTMEYLKKEIHTDTSKSTGGPHIVSMETLQYGNRVLKPGGSATFVVGY
                     MASPGSSFGEIHPDQEEALRKKRVDGILDLLRLETPDTVLNTAFAFAKIRATESIYKT
                     RGGYMHSPGGLSYYAAIWANDQAEYVNPFFAMLGDKTAILSAMNSYRLFASYMNPEFK
                     PIPSSIVAEGDSIWNGAGDRGDMAMIAYGASRFALSLGDQDSAATLWPLIEWCLEYCR
                     RKINADGVVESDTDELERRFPSGKANLATSSLYYDALRSASMLAHQLDKPSSVASGYL
                     SRAKTMKENIEKYFGATVEGFKTYRYYQGNDVLRSWICLPLTM"
BASE COUNT          358 a          379 c          403 g          373 t
ORIGIN      
        1 aagatatttt tccggttttc tccttctttt tcttgtggcg gacctttgcc gtgcgcaggc
       61 cgtattgtcg gagcagcagg accgctgggt gatcactccc gatagcagca ttgaatggaa
      121 gatagccggc cggctgccgc atcacgatca tattgaaatg agcggtgaaa aagtttccct
      181 gtgggttcaa tacggcgtag acagctccgg attgccggag gtttcgagaa cggtcgtttt
      241 cccgaccttc agaacaaagc ccaatgatac acacgccagc ctgatgtata cgtttacgga
      301 cgctgatctt ccgaggatat acatcaatgc ggaaccctta agggcggata tcatcaatgg
      361 aaggaagcgg ggaaattttc cccggcaggt ggaaagtatc cggcaaaaag ggattatgcg
      421 cattgaaagc aatatcggcg ccccttcttt cctgcacctg gaacacagcc tgttcccttc
      481 cgtggataaa gcgctggccc tggaaacttt tgtttttacg aatacctctg cgaagcaagc
      541 ctctgtaacc atggaatacc tgaaaaagga gatccatacc gatacctcaa aaagcacggg
      601 cggtccgcat atcgtcagca tggaaacgct gcaatacgga aaccgcgtgc tgaagcccgg
      661 cgggtctgct acttttgtgg tgggctatat ggcctctcct ggttcttcat tcggggaaat
      721 ccatccggac caggaagaag cgttgaggaa gaaacgggtg gacgggatcc tggacctgct
      781 gcgtctcgaa acacccgata ccgttctgaa tactgctttt gcgtttgcca agatcagggc
      841 aacggaaagt atttataaaa ccaggggcgg ttatatgcat agcccaggcg gcctttctta
      901 ttatgcggcc atctgggcaa atgatcaggc ggaatacgtc aacccgtttt ttgcgatgct
      961 gggagataaa accgccattc tttccgctat gaattcctac cggcttttcg cttcctacat
     1021 gaatccagag tttaaaccga ttcccagttc gatcgttgca gaaggcgact ccatctggaa
     1081 cggggccgga gaccgcggag atatggcgat gatcgcttac ggggcttccc gttttgcttt
     1141 gtccttaggc gaccaggatt cggccgcaac actttggccc ctgattgaat ggtgtcttga
     1201 atattgccgg cgtaaaatca atgcggacgg cgtcgtggaa tcagatacgg acgaactgga
     1261 gcggcgtttc ccttccggga aggcaaatct tgccacttct tcgttgtatt acgatgcgct
     1321 acgctctgcc tcgatgctgg ctcatcaact ggacaaaccg tcttcggttg cctccggata
     1381 cctttcccgg gcgaaaacca tgaaggaaaa tattgagaaa tattttggtg caaccgttga
     1441 agggtttaag acctataggt attaccaggg caatgacgtg ctgcgttcct ggatatgcct
     1501 gccgctcacg atg
//
LOCUS       DASYCU010000354         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_658165, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000354 DASYCU010000000
VERSION     DASYCU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658165"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VGM24_07195"
     CDS             complement(<1..795)
                     /locus_tag="VGM24_07195"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY4155194.1"
                     /translation="MNYQQITDLLGKENETLFTHVCQKVPKASLQLPSPHFTAEVWSA
                     SNRNLQTLRSLQQMFDNGRLARTGYLSILPVDQGVEHSAGASFAKNPMYFDPENIVRL
                     GLEGGCNAVASTFGVLAMMARKYAHRIPFIVKINHNELLTYPNKYDQILFGTVKDAWN
                     MGAVAVGATIYFGSEESDRQITQISQAFDEAHALGMATVLWCYLRNKAFVKDGKDYQD
                     SADLTGQANYLGATIQADILKQKLPSLNGGYKALNMGDSHYGKLDER"
     gene            897..1916
                     /locus_tag="VGM24_07200"
     CDS             897..1916
                     /locus_tag="VGM24_07200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY4155195.1"
                     /translation="MIRLSILDQSIVPKGSTASKAIANTIALVKQAEASGFHRFWVSE
                     HHNSPLIAGSAPELLMVRLANETSTIRIGSGGIMLPNHSALKVAENFRLLESMYPGRI
                     DLGMGRAPGGDRMTAVLLNPSNNFLEESYLQQLEYLQAFFHDEAETQYGPMLAVPQTV
                     SVPAPWILSSSGGSAAIAAKMGMGLAVARFINGFAGREIVENYKETFRPSREFAEPQT
                     LLAVSVLCADTPGKAAQLRKLADYTLLQFEKGNFREMSRYEDIRDYVFSPAELDRIRY
                     NSGRIISGTPDEVKQQLLELSQNTGVDEIMITSMTHSQEDRIRSFELLAEVFELANGK
                     GIVRH"
     gene            complement(1946..2899)
                     /locus_tag="VGM24_07205"
     CDS             complement(1946..2899)
                     /locus_tag="VGM24_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY4155196.1"
                     /translation="MMNNNLSKTPRPGSVILAFITVYIVWGSTYFFIRHALNGFPPLM
                     LGAFRFILAGLIMMCWAGYKKEKLLDWKAMKMAAISGFFILFIGNGVVIYVEQYVSSA
                     WVAIIVSAAPIWFVLYDKPHWKENFGSKSILAGLAMGIMGVILLFSENISSLGTNANN
                     YQTLAFISLIIGSMSWVIGSLFAKYSKPRFPAVISTTWQMFFAGIYFLAGSIAKNEFR
                     SFHLDQVSLNAWASLWYLILFGSILAYSCYVWLMEVRPPSQVSTYAYVNPVVAVLLGV
                     FLASEHISLLQITGLLVILLSVLLINLKKYLQRNGQWRVEH"
     gene            3047..3535
                     /locus_tag="VGM24_07210"
     CDS             3047..3535
                     /locus_tag="VGM24_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY4155197.1"
                     /translation="MELDRISWSIISSLQENARASYADIGRTVGLSAPAVAERMQKLE
                     ESGIINGYHIDLDYERTGYALKAFISFTAHSGKLVTFLKYIEKMDEVLECHRVTGNYC
                     IFLKVVVKDSQQLEQLLSRFMEYGETTTSIILSSPISHRVFSKSDAGLTKTNRAAKLR
                     RV"
     gene            complement(3495..>3816)
                     /locus_tag="VGM24_07215"
     CDS             complement(3495..>3816)
                     /locus_tag="VGM24_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEY4155198.1"
                     /translation="KYNMSLMTKCWAEEFKEDGIASNTLWPATTIATAAVKNIMGGEE
                     LIRRSRKPEIVADAAFYILQKDAKTNSGNSYLDEEVLRSEGITDLEPYSIIPGGVLQP
                     DLFL"
BASE COUNT         1022 a          926 c          924 g          944 t
ORIGIN      
        1 tctttcatcg agttttccgt aatgactgtc tcccatattc agggctttgt agccgccgtt
       61 aagcgagggt aacttttgtt tcaggatgtc tgcctgaatg gtcgctccaa gatagttggc
      121 ctgaccggtt aaatcagcgg aatcctgata atcctttccg tcctttacaa aagctttatt
      181 ccgcaaataa caccagagca cggtagccat gcccaacgca tgcgcttcgt cgaaagcctg
      241 agagatctgg gtgatctgcc ggtcagattc ttccgaacca aagtagatgg ttgcgcctac
      301 agcaacagcg cccatgttcc aggcatcctt aaccgtaccg aaaaggatct gatcatactt
      361 gttcggatac gtgagtaact cattgtggtt gatctttacg ataaacggaa tacggtgggc
      421 atatttccgg gccatcatgg ctaaaacacc gaaggtgctc gccacggcgt tgcagcctcc
      481 ttccaatccc aaccgcacaa tattttccgg atcgaaatac atcggatttt ttgcgaaaga
      541 cgctccggcg ctgtgttcca cgccctgatc aacaggcaga atggaaaggt agccggtgcg
      601 ggcaagccgt ccgttatcaa acatctgctg caggcttctg agggtctgta aattccggtt
      661 ggaagcactc cagacttctg ctgtaaaatg gggagaagga agttgaagcg atgccttcgg
      721 aaccttttga cagacatggg taaatagtgt ttcattttct ttacccagta aatcggttat
      781 ttgctgatag ttcataataa aaaaactgaa cctaaaatta agacatccgg tagcaaaccg
      841 gtttactttt tacaacaaag gcgcaattaa aatgttaatg atgcaaaagg aattccatga
      901 tcaggctcag catccttgac cagtccatcg ttccaaaagg ttcaacggct tcaaaagcca
      961 ttgcgaatac catcgccctg gtcaaacagg ccgaagcatc cgggtttcat cggttttggg
     1021 tttccgaaca tcataattct cccctgattg ccggatcggc acctgaatta ctcatggtaa
     1081 gactcgcgaa tgaaacatca accatccgca tcgggtcggg gggaatcatg ctgcccaatc
     1141 acagtgcact gaaggtcgcc gagaatttcc ggctcctgga atccatgtat ccggggcgga
     1201 ttgatctggg aatgggtcgg gctccgggcg gagaccggat gacggcggtc ctgctcaatc
     1261 cttccaataa ttttctggag gaatcctatt tgcaacaact ggaatatctg caggcctttt
     1321 tccacgatga agcagaaacc caatatggtc ctatgctcgc cgttcctcaa accgtttccg
     1381 tgccggctcc gtggatcctg agttccagcg ggggaagcgc agccatcgcc gcaaaaatgg
     1441 gaatggggct cgccgtagcc cgcttcatca acggatttgc tggccgggag atcgttgaaa
     1501 attataaaga aacgttccgg ccatcccggg aattcgccga accgcaaacc ctgctggctg
     1561 tttcagtgct ttgtgccgac accccgggta aggccgctca gttacgtaaa ctggctgatt
     1621 acaccttgtt gcaatttgaa aaaggaaatt tccgggagat gagccggtac gaagatatcc
     1681 gggattatgt tttttcaccc gctgaactgg acaggatccg gtacaacagc ggacggataa
     1741 tttcaggcac tccggatgag gttaaacaac aattgcttga gctgtcccaa aatacgggcg
     1801 tggatgaaat tatgattact tccatgaccc attcccagga ggaccggatc cgctctttcg
     1861 agctgctggc ggaagtcttt gaattggcaa acggaaaggg gatcgtgcgg cattgaaaaa
     1921 gaaagaccat tgtttccggg aatgctcaat gttctactct ccattggcca ttccgctgaa
     1981 gatatttctt caggttgatc agcaatacgc tcaataatat caccagcagg ccggttatct
     2041 gcagcaggct tatatgttca gaagcaagaa aaacgcccag taaaaccgct accaccggat
     2101 tcacgtaggc ataagtgctt acctgggaag gcggccggac ctccatcagc cacacataac
     2161 agctgtaagc caggatagaa ccgaagagga tcaggtacca gagagatgcc catgcgttta
     2221 aagaaacctg atccagatga aaagaacgga attcattctt ggcaattgat ccggccagaa
     2281 aatagatgcc cgcaaaaaac atttgccagg tcgtgctgat cacggcggga aaacggggtt
     2341 tgctgtattt tgcaaacagg gatccgataa cccagctcat ggatccgatg atcagggaaa
     2401 taaatgccag cgtctggtag ttattcgcat tggtccctaa tgaggaaatg ttttccgaga
     2461 atagtaaaat gactcccatg atccccatgg ccagtccggc aagaatggat ttactgccga
     2521 aattctcttt ccagtgaggt ttgtcataca gcacaaacca gatgggtgcc gcggatacaa
     2581 taatcgccac ccaggcactg ctgacatatt gttctacata aatcacgacg ccgtttccga
     2641 tgaacaggat aaaaaatccg ctgatcgccg ccattttcat tgctttccaa tccagcagct
     2701 tctccttttt gtagcctgcc cagcacatca tgatcagtcc ggccagaata aaacggaagg
     2761 ctcccagcat tagcggagga aacccgttga gagcatggcg gatgaaaaaa taggttgacc
     2821 cccacacgat ataaacggtg ataaaggcga gaataacgga accgggccgg ggcgttttgg
     2881 aaaggttgtt gttcatcatt tgtgccaggt ttaacatgcg aaacagctgt catacgctaa
     2941 atactatcca aagttacgat gagatgcagg aattttacct acgaaataat cctttttatg
     3001 tatttttacc tttgaaacaa aagtggctca acaaaaactc gtgaccatgg aattagacag
     3061 gatcagctgg tccatcatca gcagtctgca ggaaaatgcc cgggcgtcct atgcagatat
     3121 cggtcgcacg gtaggcttat ctgcaccggc ggtggcagaa cggatgcaaa aactggaaga
     3181 gtccggcatc atcaacggct atcacatcga cctggattat gaaagaacgg gttatgccct
     3241 gaaagccttc atcagcttta cggcccattc gggaaagctt gttacttttt taaagtatat
     3301 cgaaaagatg gatgaagtgc tggaatgcca tcgcgttacg ggcaactatt gcattttttt
     3361 gaaagtggtg gtaaaagatt cccagcagct ggagcaattg ctgagccggt ttatggaata
     3421 cggggaaaca accacttcca tcattctctc cagtcccatt tcgcaccggg tcttttcaaa
     3481 atcggatgcc ggccttacaa aaacaaatcg ggctgcaaaa ctccgccggg tataatggaa
     3541 taaggttcca gatcggtgat cccctctgaa cggagcactt cctcatccag gtaggaattt
     3601 cctgaatttg ttttggcgtc tttttgaagt atgtagaaag cagcatctgc cacgatctcc
     3661 ggtttccggc tgcgacggat aagttcttcc cctcccatga tatttttaac tgcggcagtg
     3721 gctatcgttg tggccggcca caaggtgttg gaagcaatcc cgtcttcctt aaattcttcc
     3781 gcccagcatt tggtcatgag gctcatattg tatttg
//
LOCUS       DASYCU010000355         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3267635, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000355 DASYCU010000000
VERSION     DASYCU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267635"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VGM24_07220"
     CDS             complement(<1..249)
                     /locus_tag="VGM24_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155199.1"
                     /translation="MSVSTEPGTGKAGIFPKPKLSFWQIFNMSFGFLGIQFGFALQNG
                     NASGILRNYGADIDKLSWFWLVAPVVGIVVQPIVGHYSD"
     gene            complement(268..1542)
                     /locus_tag="VGM24_07225"
     CDS             complement(268..1542)
                     /locus_tag="VGM24_07225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY4155200.1"
                     /translation="MTNKVLAVILGGGAGTRLYPLTASRSKPAVPIAGKYRLVDIPIS
                     NCLNSGIDRMFVLTQFNSASLNRHIKNTYHFSAFSKAFVDILAAEQTPDNPTWYQGTA
                     DAVRQGLRHLGPFEGEYILILSGDQLYQTNFMDMINNHINQQADITIATIPVPAREAS
                     DFGIMKMDDKGYITSFIEKPKKDLLKDWESDTGPAMQAKGRKYLASMGIYIFNRQLLQ
                     DLLLNEYVDATDFGKEILPQSLGKYKVASYQYDGYWTDIGNIYSFFEANLGLTNEIPD
                     FNLFDNNNSIYTRARMLPPAKISGTTLEKTIIAEGCIINASRIENSVIGIRTRIGSGT
                     TIVSSYLMGIDFYETIEEIGHDKEHGLPSVGIGNRCYIRNAIVDKNCRIGDDVRINGG
                     SHMENTDHSLYTVKDGIVVIKKGAVLPNGFVI"
BASE COUNT          387 a          373 c          382 g          417 t
ORIGIN      
        1 gtcgctgtaa tgaccaacga ttggctgaac tacgatccca acgaccggag cgaccagcca
       61 aaaccaggag agtttgtcaa tatccgcgcc gtagttacgg agtatgccgc tggcgtttcc
      121 gttttgcagg gcaaatccaa actggatccc aaggaaccca aaactcatat tgaatatctg
      181 ccagaaggat aatttcggtt tgggaaaaat acccgccttt cccgttccgg gttccgttga
      241 aactgacata agcaatcgtt gactggttta gattacaaaa ccattcggaa gcacggctcc
      301 ttttttaatg accacgatcc cgtcctttac ggtatataaa gaatgatctg tattttccat
      361 gtgcgaacct ccgttgatgc gcacatcatc cccaatgcgg cagttcttgt cgacgatggc
      421 attgcggata taacagcggt tgccgattcc aacggagggc aggccatgct ctttatcgtg
      481 tccgatctcc tcaatggttt cataaaagtc gatccccatc aggtagctgc ttacaatggt
      541 ggtgccgctg ccgatccggg tgcggatacc gatcacgcta ttctcaatgc gggaggcatt
      601 gatgatgcag ccttccgcaa tgatggtctt ttcgagcgtg gttccgctga tcttggccgg
      661 cggaagcatg cgcgcccggg tataaatcga attgttatta tcaaagaggt tgaaatccgg
      721 aatttcattg gtcaggccca ggttggcttc aaagaatgaa taaatattcc cgatatccgt
      781 ccagtagcca tcgtactgat aactggccac cttgtatttt cccagcgatt gaggaaggat
      841 ttcttttcca aaatccgttg catccacgta ttcattcagc agaaggtcct gcaatagctg
      901 gcggttgaaa atatagatac ccatggaagc caggtatttt cttcccttgg cctgcatggc
      961 cggaccggta tcgctttccc agtccttcag taaatccttt ttgggctttt cgataaagga
     1021 agtgatatat cctttatcat ccattttcat gatcccgaaa tcagacgctt cgcgggcggg
     1081 aacaggaatg gtggcaatag tgatgtctgc ctgctgattg atatggttgt tgatcatatc
     1141 cataaaattc gtctgataga gctggtcgcc ggaaaggatg aggatatact ctccttcaaa
     1201 gggacccaga tgacgaaggc cctggcgaac ggcatctgcc gtcccctgat accaggtggg
     1261 gttatccggt gtttgctcgg ccgcgaggat gtccacaaac gctttgctga atgcactgaa
     1321 gtgatatgta ttttttatat ggcggttcag cgaagccgaa ttgaattgcg tcagcacaaa
     1381 catccggtcg atcccggaat tcaggcagtt tgaaatgggg atgtctacca gccgatattt
     1441 tcctgcaatc ggaacggcgg gtttggaacg actggcagtt aatggatata accgggtacc
     1501 tgcaccacca cccagaatca cagctaacac tttatttgtc ataacgggta tatttaata
//
LOCUS       DASYCU010000356         6679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_513467, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000356 DASYCU010000000
VERSION     DASYCU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513467"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VGM24_07230"
     CDS             <1..1035
                     /locus_tag="VGM24_07230"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4155201.1"
                     /translation="QWIPNDNPVTNSVSGKVNAVYSFGHRNAQGLVWAHVGSQYILYN
                     SEHGDKSDDEVNVISAGRNYGWPKVAGMCDDNYNDTDANPNNDKLANQTIHNEHSFCS
                     ANNVAEPLFSFFNVNGSAVPSSGVSNYTWPTIAPSSIDYYDKMYIPGWKNSLLVTSLK
                     LGMYRLKLKTDGLSVDSSSTAQITDTIAYLHEYRIRDLAIAPTGDTIFLAIDSSGSTS
                     GPTGGFTGKQIATKTPGHILRMVYLTVLPDGAKEPIHPKTEKINTTLVYPNPATDHIH
                     VLCRDILQSPFRVQLFDMSGRIILSQTFTGNSFDVPLRNVREGLYVFRLFDALDNVVV
                     TRKILVVSRQ"
     gene            complement(1057..2454)
                     /gene="fumC"
                     /locus_tag="VGM24_07235"
     CDS             complement(1057..2454)
                     /gene="fumC"
                     /locus_tag="VGM24_07235"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY4155202.1"
                     /translation="MDFRTEKDTLGEVKVPADAYYGAQTQRSIDNFKIAQDINRMPKE
                     IIRAFAILKKAAALTNLEAAVLSKDKSDLIGKVCDEILEGRLDAHFPLVVWQTGSGTQ
                     SNMNVNEVIANRGHVLQGGKLTDKEKALHPNDDVNKSQSSNDTFPTAMHIAAYKILVE
                     TTIPGIETLRDTLLEKSRKYRQLVKIGRTHFMDATPLTVGQEFSGYASQLTHGLKAIK
                     NTLPHLAELALGGTAVGTGINTPPHYSEKVAAQIARLTGLPFVTAENKFEALAAHDAI
                     VETHGALKTIAVSLMKIANDIRMLASGPRSGIGELHIPDNEPGSSIMPGKVNPTQCEA
                     LTMICAQVLGNDVAINIGGATGHFELNVFKPVIIYNFLHSARLIGDGTVSFNDKCAAG
                     IEPIEANIKKHVDNSLMLVTALNPKIGYYKAAEIAQKAHQEGTSLKEMAVKLGYLTPE
                     QFDEWVDPKKMVGEI"
     gene            complement(2546..4099)
                     /locus_tag="VGM24_07240"
     CDS             complement(2546..4099)
                     /locus_tag="VGM24_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY4155203.1"
                     /translation="MSDQTGFLNSIRNGYYFKSESVKIGIGMLDGKPVPGADINLPLK
                     TMNRHGLIAGATGTGKTKTLQMITEALSNSGVPVLVMDIKGDLSGLGAAGTENDQLKS
                     RCDSIGIHFQPRAFPVEFLTLSNEKGVRLRATVSEFGPILLSRILELNDTQEGLVAMI
                     FKYCDDNKLPLLDLKDFIKVLQFISAEGKAEIQQAYGSIATTSTGTILRKVIGLQQQG
                     ADVFFGEKSFEVADLMRQSDDGRGMVNILRVTDLQDKPKLFSTFMLQLLAELYGSCPE
                     EGDPDKPKLLMFIDEAHLVFEEASDALLKQIETIVKLIRSKGIGIFFCTQNPADVPSG
                     ILAQLGLKVQHALRAFTASDRKAIKQAAENYPSTTYYKTEDLITGLGIGEALVTMLDE
                     KGVPTPLVHCLMAPPASRMDILRPEEIDALVAKSKLVPKYNQVIDSNSAYEILTQKLQ
                     DAAAKGQSAPAKPPPAGGKPQPTTLEKILNSGAARQVERTAASVITRGLLGALGLGGR
                     SSSKKSGWF"
     gene            complement(4149..5204)
                     /gene="lpxD"
                     /locus_tag="VGM24_07245"
     CDS             complement(4149..5204)
                     /gene="lpxD"
                     /locus_tag="VGM24_07245"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY4155204.1"
                     /translation="MQFSAVQIAGLVNGKVEGDPEVSVDAFGKIEEARSGQLAFLANP
                     KYEDHLYTTKASIILVNESQVLKEKINATLIRVPDAYLAFAALLSRYQEMMKEQLTGI
                     QEPSYISKTAVTGEQVFVGAFAFIGEQVRIGNNVKIYPQVYLGDRVRIGDESVILPGA
                     KIYSDCVIGKGVTIHAGCVIGSDGFGFAPQPDGSFRKIPQMGNVVVEDFVEIGANTTI
                     DRATIGSTLIRSGAKLDNLIQIAHNVEVGNNTVIAAQAGVSGSTKLGRNVMVGGQVGI
                     VGHLQIADGSRINAQSGVTKSLKTPNSAVTGSPAYDYTSALRSQVASRHLPDFEKRLQ
                     ELEKKIRELLVGQDLKS"
     gene            complement(5247..6458)
                     /locus_tag="VGM24_07250"
     CDS             complement(5247..6458)
                     /locus_tag="VGM24_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY4155205.1"
                     /translation="MRAGSQKIINDPVYGFITIDHPLILDLIRHPYFQRLRRIQQMGL
                     AHLVYPGAVHTRLHHGLGAYHLMCTALNTLKGKGIDITESEELGAKAAILLHDAGHGP
                     FSHALENNLIEKVHHETVSLQIMQQLNEETGGRLKTAIEIFTDSYPKRFLHQLVSGQL
                     DVDRMDYLNRDSFFTGVSEGVIGYDRIIHMLTVHEGELMVQEKGTYSIEKFLVARRLM
                     YWQVYLHKTVVSAESMLVKIIQRAKELIAMGEHLYSGSSPLDFFLSHSVSDPGEYLKP
                     FSLLDDYDVMSAIKLWMNHGDKVLSALSVMLINRQLLKVKLQAEPFDEKITEAHRARA
                     VRELNLRPEEADYFAFTGENTNSTYNPEDERINILFKDGEVRDISRVDNALISRSLAT
                     EVKKFYICYLA"
BASE COUNT         1472 a         1736 c         1722 g         1749 t
ORIGIN      
        1 cagtggatcc ccaatgataa cccggtaacc aattccgtga gcggaaaagt gaatgccgtg
       61 tattcttttg gtcaccgtaa tgcccaggga ctcgtgtggg cacatgtggg ctcgcagtat
      121 attttatata attcagagca tggggataaa tcggacgatg aggtgaatgt gatcagcgcg
      181 ggaagaaatt acggctggcc caaagtagcc gggatgtgtg atgataatta caatgacacc
      241 gatgccaacc cgaacaatga taaactggcc aaccagacca ttcataacga acacagcttt
      301 tgttcggcca ataatgtggc ggaacccctg ttttcatttt ttaatgtgaa tggttcggct
      361 gttccttcca gcggcgtttc caattatacc tggcccacga ttgctccgtc cagtattgat
      421 tattatgata aaatgtatat tcccggatgg aaaaattctt tactggtgac ctcccttaag
      481 ctgggcatgt accggctgaa gctcaagacc gacgggctgt cggtggatag ctccagtacg
      541 gcgcagataa cggacacgat cgcttacctg catgagtacc ggatcaggga cctggccatt
      601 gctcccacgg gagacacgat ttttcttgcg atcgacagca gcggttccac gtcagggccg
      661 accggcggct ttaccggaaa acagatcgcg acaaaaacgc ccggccatat tctgcgcatg
      721 gtttacctga ccgttcttcc ggatggagca aaagaaccaa tccatccgaa aaccgaaaaa
      781 atcaatacca cgctggtcta tccgaatccg gcaacggatc atattcatgt tctctgccgg
      841 gatattctac agagcccttt ccgggttcag ttgtttgata tgtccggaag gatcatcctt
      901 tcacaaacat tcaccggaaa tagttttgac gtgcctctgc gaaacgtccg ggagggattg
      961 tatgtattcc gtttgtttga tgcgctggac aatgtagtgg tcaccaggaa gatcctcgtg
     1021 gtgagcaggc aataaatgct ggtgaataaa cagggattaa atttccccta ccatcttttt
     1081 cggatccacc cattcgtcga attgttccgg cgtcaggtat ccgagtttta cggccatctc
     1141 tttcaggctg gttccttcct ggtgcgcctt ctgtgcaatc tctgccgctt tataatagcc
     1201 gattttcgga ttcagtgcgg tcaccagcat cagggaattg tccacgtgtt ttttaatatt
     1261 ggcttcgatg ggttcgatcc ccgccgcgca tttatcgttg aagctcaccg tgccgtcgcc
     1321 gatcaggcga gcgctgtgca gaaagttgta gatgatcacc ggtttaaaaa cattcaactc
     1381 aaaatgcccg gtagctccgc cgatattgat cgcaacatca ttgcccaata cctgtgcgca
     1441 gatcatggtc agggcctcgc actgggtggg gttcactttt cccggcatga tggaggatcc
     1501 gggttcatta tcggggatgt gcagttcacc gatgccacta cggggaccgg aggccagcat
     1561 gcggatatca ttggcaattt tcatcaggct tacggcaatc gtttttagag cgccgtgcgt
     1621 ttccacaatc gcgtcgtgtg cggcgagcgc ttcgaatttg ttttccgccg taacaaaagg
     1681 aagcccggta aggcgggcaa tctgagccgc caccttttcg gaataatgcg gcggggtgtt
     1741 aatgcctgtg ccaacggccg taccgcccag ggccagctca gcgaggtggg gcagcgtatt
     1801 tttaatggcc ttcaacccgt gcgtcagctg ggaggcataa ccgctgaatt cctgcccgac
     1861 ggtcagaggc gttgcatcca taaaatgggt ccggcctatt ttaaccagct gccggtattt
     1921 cctgcttttt tcaagcaggg tatcccgcag ggtttctata ccgggtatgg tggtctccac
     1981 cagtatttta taggcggcaa tatgcatggc cgtgggaaag gtatcgttgg acgactggga
     2041 cttatttaca tcatcattgg gatgaagtgc tttttccttg tccgtcagtt tgccgccctg
     2101 cagcacatgc ccccggttag cgatcacttc attcacgttc atattactct gcgtgccgct
     2161 tccggtttgc cagaccacca gcgggaaatg cgcgtccagc ctgccttcca ggatctcatc
     2221 acaaacttta ccgattagat ctgatttatc ttttgaaaga actgccgcct ccaggttggt
     2281 gagggccgcc gctttcttga gaatggcaaa tgcgcggatg atctctttgg gcatccggtt
     2341 tatatcctgt gctattttga aattatctat actgcgctgg gtttgtgcgc cgtagtaggc
     2401 gtctgcaggt accttcacct cacccagggt atctttttct gtccggaaat ccatgtgatt
     2461 atttgaagac gcaaaattag tcacaaatcg gaacaacttc gctatttcga tattgcgggc
     2521 gcgggcggta agggcaggga aagcgttaaa accagcccga ttttttagaa ctgcttcttc
     2581 ctcccaaacc cagcgcgccc agcaggcccc gggtgatcac gctggcggcc gttctttcaa
     2641 cctgccgcgc cgcgccgctg ttcaggattt tttccagcgt ggtgggttgc ggttttccgc
     2701 cggcgggagg aggtttggcc ggagcggact gacctttggc ggccgcatcc tggagttttt
     2761 gtgtcaggat ctcgtaggcg ctattgctgt caatgacctg gttgtatttg ggcaccagtt
     2821 tggattttgc gaccagggcg tctatctctt cgggacggag aatatccatc cggctggcgg
     2881 gaggggccat caggcagtga accaggggag tgggaacgcc tttttcatcc agcatggtta
     2941 ccaatgcctc cccgatgccc agtccggtaa tcaggtcttc cgttttataa taggtcgtgc
     3001 tgggataatt ttctgcggcc tgcttaatgg ccttgcgatc gcttgccgtg aaagcccgta
     3061 aggcgtgctg aacttttaat ccaagctggg ccagaatgcc ggaagggaca tcggccgggt
     3121 tttgcgtgca gaaaaaaatg ccaatgcctt tggaacggat gagttttaca atcgtctcaa
     3181 tctgtttcag cagtgcgtcg ctggcttctt cgaataccag gtgcgcctca tctataaaca
     3241 tcagcagttt gggtttgtcc ggatcacctt cttcggggca ggagccatag agctcggcca
     3301 gcagctgcag catgaaagtg gaaaaaagct tgggtttgtc ctgcaggtcc gttacgcgca
     3361 atatattcac cattccgcgc ccatcatctg actggcgcat caggtcggcg acttcaaaac
     3421 ttttctctcc aaaaaaaaca tccgcaccct gctgctggag tccgatcacc tttctcagaa
     3481 tggttccggt ggaagtggtg gcgatcgacc cgtaagcctg ctggatctcc gccttgcctt
     3541 cggcgctaat gaactgaagc accttgatga aatctttaag gtccagcagc ggcagcttgt
     3601 tgtcgtcgca atatttgaag atcatggcta ccaggccttc ctgggtatcg ttgagttcca
     3661 gtatccggga taaaaggatt ggtccgaact cactcacggt ggcgcgcaaa cgaactcctt
     3721 tctcgttgct gagggtaaga aactccaccg ggaaagcccg gggttgaaaa tggattccga
     3781 tgctgtcgca gcggcttttg agctgatcgt tctccgttcc ggcagcgcca agtccgctca
     3841 ggtctccttt tatatccatc accagcacgg gaacaccgga attgctcaat gcctccgtga
     3901 tcatctgcag ggtcttggtt ttccccgtac ccgtggcccc ggcgatcagt ccgtgccggt
     3961 tcatggtctt tagcggcagg ttgatgtctg cgcccggaac gggttttccg tcgagcatgc
     4021 cgatcccgat ctttacggat tcacttttga aatagtaccc gttgcggatg ctgtttaaaa
     4081 aaccggtctg atccgacata aaatataaaa gtttaaaata aaatcctcct tttttccatg
     4141 cacaacgctc aactttttaa atcctggccg accagtaact ccctgatctt tttctccagc
     4201 tcctgaaggc gtttttcgaa atccggcaaa tgccggctgg ccacctggct tcgcagggcg
     4261 ctggtataat cataagccgg actgcccgtc accgcgctgt tgggcgtttt cagcgatttg
     4321 gtcacgccgc tttgcgcgtt gatccggctg ccatcggcaa tctgcaaatg gcccacgatg
     4381 ccgacctggc cgcccaccat cacatttctg cccagcttgg tgcttccgga aacgcccgcc
     4441 tgcgccgcta ttacggtatt gttgccgact tccacattgt gtgcgatctg aatgagatta
     4501 tccagtttgg cgccgctgcg gatcagggtg gatccgatcg ttgcccggtc aatcgtagtg
     4561 ttggcgccaa tctccacaaa atcttccacg accacatttc ccatctgcgg gatcttcctg
     4621 aaactgccgt cgggttgcgg agcaaatccg aatccatcgc tgccgatcac acaaccggcg
     4681 tgaatggtta cgccttttcc gatcacgcag tcggaatata tttttgcgcc gggaaggatg
     4741 accgattcat ctccgattct tacccggtct cccaggtata cctgggggta gatctttaca
     4801 ttgttgccga tccgtacctg ctcgccgata aaggcaaagg cgcccacgaa aacctgttcg
     4861 ccggtgaccg ccgttttcga aatataggag ggttcctgaa tgccggtcag ttgttctttc
     4921 atcatttcct gatacctgga cagcaaagcg gcaaatgcca gataggcatc cggcacccgg
     4981 atcagggtgg cattgatttt ctctttgagc acctggctct cattcaccag aatgatggag
     5041 gcttttgtgg tataaagatg gtcttcatat tttggattcg ccaggaatgc cagttgaccg
     5101 gagcgggcct cttcgatctt tccgaaagcg tctacggaaa cttccggatc cccttccacc
     5161 ttaccgttga ccagtcccgc gatctgcaca gcagaaaatt gcatatttaa tttgtttgga
     5221 atgaaaacag ccgtttaccg gattccctac gcaaggtaac aaatatagaa ttttttcacc
     5281 tcggtggcca ggctccggct gatcagggca ttgtctaccc gggagatatc ccggacttcg
     5341 ccgtccttga agagaatgtt tatcctttca tcctccgggt tgtatgtgct gtttgtattt
     5401 tctccggtaa aagcaaaata gtcagcctct tccggacgca ggttcagctc ccgcaccgcg
     5461 cgcgcgcggt gggcttccgt aattttttca tcaaagggtt ctgcctgcaa tttcactttc
     5521 agcagttgcc ggttgatgag catgacgctg agggcggaca aaaccttatc cccatgattc
     5581 atccataatt ttattgcgct catgacgtcg taatcgtcca ggaggctaaa cggtttcagg
     5641 tattccccgg gatccgatac ggaatgggaa agaaaaaaat caagggggga tgaaccggaa
     5701 tacaaatgtt cccccatcgc gatgagttct tttgcccgct gaatgatttt caccagcatg
     5761 ctttccgcgc tcaccacggt tttatgcagg taaacctgcc agtacatcag gcgccgggcc
     5821 accaggaatt tttcaatgga ataagtccct ttttcctgga ccatcagttc cccctcgtgc
     5881 acggtcagca tatgaatgat ccggtcgtac ccgatcaccc cttccgaaac gccggtaaaa
     5941 aaactatcgc ggttcaggta gtccatccgg tccacatcca gttgtccgga aaccagctga
     6001 tgtaaaaacc gtttggggta tgaatcggtg aaaatctcaa tggctgtttt gagccggccg
     6061 cccgtttctt cgttaagctg ttgcatgatc tgcagcgaaa ccgtttcatg atgcactttt
     6121 tcgatcagat tattttccag ggcgtgggaa aaaggtccgt gtcccgcatc gtgcagcagg
     6181 atggccgctt tggccccgag ttcttcggat tccgtgatgt caatgccttt gccttttagc
     6241 gtattcagcg cggtacacat caggtggtaa gcgcccagcc cgtgatgcag gcgggtatgc
     6301 acggcccccg gataaacaag atgggccagt cccatttgct ggatgcgccg caggcgctga
     6361 aaatacggat gccggatcag gtccaggatc agcggatggt caatcgtaat gaaaccgtaa
     6421 acgggatcat tgatgatttt ctgggaaccg gcacgcatgc tgtatttgat tttgttaaaa
     6481 gaagccaaat cctggcccaa atttaggaat gactgactga taaactatct ttagaataac
     6541 gaaacatcat actatggcac caggaacgat actttgggtg gacgatgaga ttgagagcct
     6601 gcaatcacat atcctgtttt tgaagagtaa gggttatgaa gtgaatgcga tgaccaacgg
     6661 gttggacgcg attgacttt
//
LOCUS       DASYCU010000357         4290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_367153, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000357 DASYCU010000000
VERSION     DASYCU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367153"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VGM24_07255"
     CDS             <1..518
                     /locus_tag="VGM24_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4155206.1"
                     /translation="QGIYSSGGANSFLNLILYLVEKYAGRDMAIIASKVFEIEIERQT
                     QSPFIIFGGQKDHDDEPILRAQAFIENNFQEKISVEQLASMLAISRRNLERRFKKATS
                     NTAVEYMQRVKIEAAKQSLESSRENVNEVMYKVGYSDTKAFRNAFKRITGLSPVEYRN
                     KYNRENIPAYS"
     gene            complement(545..922)
                     /locus_tag="VGM24_07260"
     CDS             complement(545..922)
                     /locus_tag="VGM24_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4155207.1"
                     /translation="MRRDVFQAIADPTRREILHLISRQPLNVNKVSEKFEVSRTAIYK
                     HIKILTECGLVTIKPEGRERFCEARLEKLHEVSDWVDQYRKIWTAKLDRLEDYLKELQ
                     SANKRTVKIKSRNKKESSGKGRK"
     gene            1083..1532
                     /locus_tag="VGM24_07265"
     CDS             1083..1532
                     /locus_tag="VGM24_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155208.1"
                     /translation="MKKIILSAAIVGALLMFNQTQAQPEISLNVRVGAPPVWIPQGID
                     NTDYYYLPDIQTYYYIPRHQFIYMEGGRWMFSASLPGRYRSYDLNRGYKVVVHEPRPY
                     LHDNIYRERYGRSGYNYNRPEYRNGNGYSRYGRSSEPGRGHAYGHRR"
     gene            complement(1546..2922)
                     /locus_tag="VGM24_07270"
     CDS             complement(1546..2922)
                     /locus_tag="VGM24_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155209.1"
                     /translation="MNTNRLILAAILFCFSSSVQAQHKHGAADSLNRPVEDTMDMPGM
                     NMHDMHHMAMEGMSMPMNHSLSLNLPMSRDGSGTSWAPDASPMYGYMVHARSWMFMFH
                     GDIFPRYTMADLFKKGKRGAAKWDAPDMLMAMGQRKIGTAGLFHFNVMLSADALIAGG
                     SGYPLLFQTGESWKGRPLVDRQHPHDLFSELSISYAYALSKRSDLSVYLGYPGEPALG
                     PVAFMHRPSGLFMPDAPIGHHWADATHISFGVATIGFRFYNFKLEGSSFTGREPDENR
                     YNFDKPRFDSWSGRLSYNPTENWSFQVSRGFIKSPEALQPGENINRTTASATWVSRFN
                     DKSHIASTALWGQNKIAGQPSSNAALLEATVKLNRLVLYTRYEWVQKSVEELALDPVI
                     YGNAEWLFPVNAVSLGLGYDLFRIGHLIVAGGGQLSLYKTSDALNSLYGATPLSGQIY
                     LHIYPGRM"
     gene            complement(2988..4106)
                     /locus_tag="VGM24_07275"
     CDS             complement(2988..4106)
                     /locus_tag="VGM24_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4155210.1"
                     /translation="MRMLRYILQKEFLQLFRDRTILAMMFAMPTIQLTIIPLAMNFDV
                     KNINISVVDLDHSSLSQKLLTQVGASGYFRYVNISPSYKEGLKSIERGEADLIMEIPA
                     GFERNLVREGSQKISLSVDAINGTKASLGTGYLRTIIEDFNQTLPLQINASPAAAPQP
                     AIDITYSYWFNPLGEYKFYIVPGVLVLLLTLIGGFISALNIVKEKESGTIEQINVTPI
                     KKWQFILGKMIPFWIMAMVVFSLGLLVTWGVYGIWPQGNLALLYLFASVYLIAVLGFG
                     LLISTYSDNQLQAMFVAFFFMMIFMLMSGLFTSIESMPAWARTIANLTPITHFIKVIR
                     MIMLNGSGWADIRMELFYELLFAVVLNAWAIWNYKKTA"
     gene            complement(4103..>4290)
                     /locus_tag="VGM24_07280"
     CDS             complement(4103..>4290)
                     /locus_tag="VGM24_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4155211.1"
                     /translation="MPRIFQLISNIVPSRWYYLIVKAVMLKGLGFEYVWKETLVLTGM
                     TVVLLAISLKNFKKRLS"
BASE COUNT         1080 a         1069 c         1077 g         1064 t
ORIGIN      
        1 agcagggtat ttattccagc ggaggagcca attctttcct gaacctgatc ctatacctgg
       61 tggagaaata tgccggtcgc gatatggcca tcatcgcctc caaagtattt gagatcgaga
      121 tcgagcgaca gacacaatct ccctttatca ttttcggcgg acagaaagac cacgatgatg
      181 agcccatcct gagggcccag gcctttattg aaaataattt ccaggagaag atcagtgtcg
      241 agcagctggc ctccatgctg gcgatcagcc gcagaaacct ggagcgccgg tttaagaagg
      301 cgacctccaa taccgcggtc gaatatatgc aaagggtaaa aattgaggct gccaaacaaa
      361 gcctggaatc ttcccgtgaa aatgtgaacg aagtcatgta taaggtcggg tacagcgata
      421 caaaggcttt tcgcaacgca ttcaagcgga tcaccggact gtcgccggtg gagtaccgta
      481 acaaatataa ccgggaaaat ataccggcat attcatagac ccggttttga tttgtaattc
      541 ctgtttattt tctccctttg ccggaacttt cttttttatt cctgcttttt attttaaccg
      601 ttcgtttatt cgcggactgc agctccttca gataatcctc gagccggtcg agtttggcgg
      661 tccagatttt ccggtactga tccacccagt cggacacctc gtgcagtttt tccagcctcg
      721 cttcgcaaaa ccgttcccgg ccctccggtt tgatggtgac cagtccgcat tcggtaagga
      781 tcttgatatg cttatagatg gccgtccggc ttacttcgaa tttttcagac actttgttca
      841 cgtttagcgg ttgacgggag atcaggtgta aaatttccct tctggtggga tcggcaattg
      901 cctgaaagac gtctcttctc atttaaatgt aacctttggg ttgcccaaat atagggaacc
      961 ttttcaatgc cgtgttttcc tgctttgttt tcaggctccc cggctgcggc ttttgctttt
     1021 ttataacatt cgcctgttat acgtttatcc tgagaggaag tccaaataga aaacctacaa
     1081 acatgaaaaa gatcatcctg tcagcagcaa tagttggcgc tcttttgatg ttcaatcaaa
     1141 cacaggcgca gccggaaata agtttgaatg tccgggtcgg tgccccgccg gtatggattc
     1201 cgcagggtat cgacaacaca gattactatt atcttccgga tatccagacc tactattata
     1261 ttccccggca ccagttcatt tatatggaag gcggaagatg gatgttttcg gcctctctcc
     1321 cgggcagata ccgctcgtat gatttgaaca ggggttataa ggtggtcgta cacgaaccca
     1381 gaccttattt gcacgataat atttaccggg aaaggtatgg ccggagcgga tataattaca
     1441 atcgtcccga atacagaaat gggaatggct attcccggta tggccggagc agtgaaccgg
     1501 ggcgcgggca cgcatatggt caccggagat aagcatcgtg cccggtcaca ttctgcccgg
     1561 gtatatatgc aggtagatct gaccagacaa aggagtggct ccgtagaggc tgttcagggc
     1621 atcgctggtt ttgtacagcg atagttgtcc cccgccggca acgatcaggt gcccgatccg
     1681 gaacagatcg tagccgaggc ccagagaaac cgcgtttacc ggaaacagcc attcggcatt
     1741 tccgtaaatg accggatcca gcgccagctc ttccaccgat ttttgaaccc actcatagcg
     1801 ggtgtacaga accagcctgt tcaatttgac cgtagcttcc agcagggccg cattggaaga
     1861 gggctgaccc gctattttgt tctgccccca gagtgccgtg gatgcgatgt gcgatttatc
     1921 gttgaaccgg cttacccagg tggcggatgc cgttgtacga ttgatatttt cgcccggttg
     1981 caaggcctcc gggcttttta tgaaaccacg ggagacctgg aacgaccagt tttcggtggg
     2041 gttataggat agccttccgc tccaggaatc gaaacggggt ttgtcaaaat tataccggtt
     2101 ctcgtcgggt tcccttccgg taaaggaaga cccttcgagc ttgaaattat aaaaccggaa
     2161 accgatggta gccacgccga agctgatatg ggtggcgtcc gcccaatgat gcccgatcgg
     2221 agcgtccggc atgaacagcc ccgatggccg gtgcataaag gcaaccgggc ccaatgccgg
     2281 ttctccggga taacccaggt agaccgagag atcggatctt ttagaaagcg cataagcata
     2341 gctgatagac agttccgaaa acaggtcgtg cggatgctgg cggtcgacca gcggtcttcc
     2401 tttccaggat tcgccggttt gaaacaggag gggatacccg cttcccccgg cgatgagcgc
     2461 atcggcggaa agcatgacat taaaatggaa caaaccggcg gtgccgatct tccgctgacc
     2521 cattgccata agcatatccg gggcatccca tttggcggcg cctctttttc cctttttaaa
     2581 aagatcggcc atagtatacc ggggaaaaat atctccgtga aacatgaaca tccaggatcg
     2641 tgcatggacc atgtatccat acatcgggga agcatcggga gcccagctgg ttccggaacc
     2701 gtcccggctc atgggcagat tcagggaaag ggaatgattc atcggcatac tcattccttc
     2761 catagccata tgatgcatgt catgcatatt cattcccggc atgtccatcg tgtcttccac
     2821 gggcctgttc aggctatctg ccgccccatg cttatgctgc gcctgaacgg aggaggaaaa
     2881 gcaaaacaaa atggccgcca gaatcaaccg gttggtattc atgaaatccg tgttatttga
     2941 agcaaagtta gcccggtgtg ggtcagcatc cttcagtggg ttccgatcta ggccgtcttt
     3001 ttgtaattcc atattgccca ggcattcagc accacggcaa aaagcagctc atagaataac
     3061 tccatcctga tatccgccca tccgctgccg tttagcatga tcattctgat caccttgatg
     3121 aaatgggtga tcggagtgag gttggcgatg gtcctggccc aggccggcat gctttcgatg
     3181 gaggtgaaca gcccgctcat cagcatgaag atcatcataa aaaagaaggc gacaaacata
     3241 gcctgcaact ggttgtcgct atacgtggag atgagtaagc caaagccaag caccgcgata
     3301 agataaacgg aagcaaaaag ataaagcagg gccaggtttc cctgcggcca gattccataa
     3361 actccccagg ttacgaggag gcccaggctg aaaacgacca tggccatgat ccagaacggg
     3421 atcatcttac ccagaataaa ctgccatttt tttatcgggg ttacattgat ctgctcgatg
     3481 gtgcccgatt ctttttcttt cacgatattc agggcagaaa taaagccgcc gatcagggtc
     3541 agcagaagta ccagcactcc cggaacgata taaaacttgt attcacccag cggattgaac
     3601 cagtaagaat acgtaatatc aatggccggt tggggcgcag ctgccggact ggcatttatc
     3661 tgcagtggaa gggtttgatt aaaatcctca atgatggtgc gaagatagcc ggtgcccagg
     3721 gacgctttgg ttccattaat ggcatccacg gaaagcgaaa ttttttgtga accttccctg
     3781 accaggtttc tttcaaagcc cgccggtatt tccatgatca gatcggcttc ccctctttca
     3841 atactcttca atccctcttt ataggaaggg gatatgttta cataccggaa gtatcccgat
     3901 gcacctacct gcgtaagcag tttttgggaa agactgctgt gatccaggtc cactacggaa
     3961 atattgatat ttttcacgtc gaaattcatg gccagcggaa taatggtcag ttgaatggtg
     4021 ggcatggcaa acatcatggc caggatggtc ctgtctctga ataactgaag gaattctttc
     4081 tgcaatatgt accgcaacat cctcatgaca gccgcttttt aaaatttttc agggagatgg
     4141 ccagcaatac caccgtcatt cccgtaagaa caagcgtttc cttccagaca tattcaaagc
     4201 cgaggccttt cagcatcacc gcctttacga tcaggtagta ccagcgggag ggaactatat
     4261 tggaaataag ctgaaaaatc cggggcatat
//
LOCUS       DASYCU010000358         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2687699, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000358 DASYCU010000000
VERSION     DASYCU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687699"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VGM24_07285"
     CDS             complement(<1..1005)
                     /locus_tag="VGM24_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155212.1"
                     /translation="MENEPISSSPLEAVRIFEFRNLVIGRFLFIMGLRMMSTLVGWWI
                     YNLTNAPLAIGIVGLSEFVPAVSFALYAGHMIDLSEKRKMMLLGVSLYGLAALLLLLL
                     STRIGSDHFHPHSIALLIYLIIFGTGVVRSFTGPLFNVVLAQVVPKKLLQNASTWNQG
                     AYLSASVTGHAFGGFLIAGLGNTGTLAIVAILVFAALSVLFGLKPKPPVNERGEKKTW
                     DSVREGLAFVFRTKELLGAISLDLFAVLFGGAVAMVPVYARDILKVGVKGFGFLNGAS
                     DLGSIAIVILLTLFPLKYQQGKKLLFAVGGFGLCIIIFGLSRLFWLSFGILVISGMLD
                     G"
     gene            complement(1031..1663)
                     /locus_tag="VGM24_07290"
     CDS             complement(1031..1663)
                     /locus_tag="VGM24_07290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY4155213.1"
                     /translation="MNYHFIAVEGNIGAGKTTLAHLLARHFNSRLILEAFAENPFLPK
                     FYENPKQYAFPLELFFMAERYKQLKELVHTKDLFRSLTISDYIFSKCLLFAKVNLPDE
                     EFHLYQKLFDIIYQQLVQPDLLIYLHSPVSQLQKNIRKRNRSYELSIPDDYLFNLQET
                     YTSYIRQHNIKTLFIDASEADFLGNEKHFQVILDALEKEYEPGQHYFTLP"
     gene            complement(1708..2187)
                     /gene="folK"
                     /locus_tag="VGM24_07295"
     CDS             complement(1708..2187)
                     /gene="folK"
                     /locus_tag="VGM24_07295"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY4155214.1"
                     /translation="MHKAYLLIGGNEGDRFLHMQQARANIEHICGVIEQVSSLYLTAA
                     WGKTDQEDFLNQALMIRTPLGPRELLRANLGIEEKMGRKRTGKNAPRIIDIDILFYDQ
                     EIISEPDLAIPHPRIPDRRFVLEPLNEIASGWIHPVSGKTVNELLRECTDTLAVKKI"
     gene            2250..>2891
                     /locus_tag="VGM24_07300"
     CDS             2250..>2891
                     /locus_tag="VGM24_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY4155215.1"
                     /translation="MRSFFKIFFASLLALIAFVLIIFFFFLGFVSSIAGSQHEETGSN
                     AVLVLDLSQSFREKPVEDPLANFSETRYNAPGVYDVIRMIRYAKSDSAVRGIYIKAGS
                     NANGLGTTEEIRNALIDFKKSGKFIYAYGDVISMKAYYAANVADKFYCNPKGGLDWQG
                     LTSVMLYIKGTLDKLGIQPEIFYAGKFKSATEPLRMEKMSDANRRQMTELLGDV"
BASE COUNT          756 a          674 c          750 g          711 t
ORIGIN      
        1 gccgtccagc atgccgctga tgaccagtat gccaaaggag agccagaata gcctggaaag
       61 accaaaaata ataatgcaga gaccgaatcc gccaacggca aacaacagtt ttttgccttg
      121 ctggtatttg agtggaaaga gcgtgagcag gatcacgatg gcaatagatc cgaggtccga
      181 agctccgttt aaaaatccga atcctttcac cccgaccttc aggatgtccc gcgcgtaaac
      241 cggcaccatg gccacggcgc cgccaaacaa taccgcgaat agatccaggg aaatggcgcc
      301 cagcaactct tttgtccgga atacaaaggc cagtccttcc cgtacgctgt cccaggtttt
      361 tttctctccc cgttcattca ccggaggctt tggttttaaa ccgaataaaa cggagagggc
      421 ggcaaagacc aggatggcca cgatagccag cgttccggta tttcccaatc cggcgatcag
      481 gaatccgcca aaagcatgac cggttaccga agcggagagg taggcgccct ggttccaggt
      541 ggaggcgttc tgtaataatt ttttagggac cacctgggca agcaccacat tgaacagggg
      601 accggtaaag gagcggacca cgccggtgcc gaaaatgatc agatagatga gcagggcaat
      661 ggaatgcgga tgaaagtgat cgcttccgat ccgggtggaa agaaggagca gaagcagcgc
      721 ggccagcccg taaagcgata ccccgagcag catcattttg cgtttttcac tcaggtcgat
      781 catatgcccg gcatagaggg caaaagaaac ggccggaaca aattcggata atcctacgat
      841 cccgatggca agcggtgcgt tggtaaggtt gtatatccac cagcccacca gcgtgctcat
      901 catgcgcaaa cccatgataa acaaaaaacg gccgatgacc aggttccgga attcgaaaat
      961 ccggaccgct tccaggggag aggaggatat cggttcattt tccattgcat gaaaatatat
     1021 taccaggcgg tcatggcaaa gtgaaataat gctgtcccgg ttcgtactct ttttccagtg
     1081 catccaggat cacctgaaaa tgtttttcat tacccaggaa gtccgcctcg gacgcgtcaa
     1141 tgaaaagcgt tttgatatta tgctgccgga tataactcgt ataggtttcc tgcagattaa
     1201 aaagataatc gtccggaatg gacagttcgt acgaacggtt ccgcttgcgg atattttttt
     1261 gcagctggct gaccggtgaa tgtaaataga tcagcaggtc gggctgaacc agctgctgat
     1321 agatgatgtc aaataatttc tggtagagat ggaactcctc gtcgggcaga tttactttgg
     1381 caaaaagcag gcatttgctg aatatataat cggatatcgt caggctccgg aacaggtctt
     1441 tggtatgcac gagttctttt aattgtttat agcgttcagc cataaagaaa agctccagcg
     1501 ggaaagcata ctgtttggga ttttcgtaaa atttaggaag aaagggattc tctgcaaagg
     1561 cttccagaat gagcctcgaa ttaaaatgcc gggccagcag gtgggcaagc gtggtctttc
     1621 cggccccgat atttccttcc acggcaataa aatgatagtt catgaagctg taaatatccg
     1681 gattccatgg ctgttatccc gggctgttca tatttttttt accgccaggg tatccgtaca
     1741 ttcccgcagc agctcattca cggttttccc cgataccggg tgtatccatc cggatgcaat
     1801 ttcattcagg ggttccagta caaagcgccg atcaggaatc cgtgggtgcg gaatggcgag
     1861 atccggttcc gaaatgattt cctgatcata aaaaaggata tcgatatcta ttattcgcgg
     1921 agcattcttc ccggttcttt tcctgcccat tttttcctcg atgcccaggt tcgcccgcag
     1981 gagttcgcgg ggcccgagcg gggtccggat catcagcgcc tggttgagga aatcttcctg
     2041 gtctgttttt ccccatgccg ccgtgaggta cagggaggaa acttgttcaa tgacaccgca
     2101 aatatgttct atatttgccc ttgcctgctg catatgaaga aatctgtcgc cctcattacc
     2161 gccaatcaat aaataagctt tatgcataaa tgatccggtt atccggatca aagatcatta
     2221 atttaaattc atatctaaac ggaaagcgca tgcggagctt ttttaaaata ttttttgcga
     2281 gcctgctcgc tttgattgct tttgttctga tcatattctt tttctttctg ggttttgtga
     2341 gcagcatagc gggttcgcag catgaagaaa ccggaagtaa cgccgtgctg gtactggacc
     2401 tgagccagtc attcagggag aagccggtgg aggacccgct ggcgaatttc agtgagaccc
     2461 gttataatgc gcccggcgta tatgatgtta tccggatgat tcgctatgcg aaatcagaca
     2521 gtgcggtgcg gggtatttat attaaagccg gttccaatgc caacggactg ggtaccaccg
     2581 aagagatccg gaatgcgctg atcgatttta aaaaatccgg gaaatttatt tatgcctatg
     2641 gcgatgtgat ctccatgaaa gcgtattatg cggccaatgt ggcggataaa ttttattgta
     2701 acccgaaagg gggattggat tggcaggggc ttaccagcgt aatgctctat atcaagggga
     2761 ctctggataa actgggcatc cagccggaga tcttctacgc cggtaaattt aaaagtgcca
     2821 cggagcctct gcgcatggaa aagatgagtg acgccaaccg gcgccagatg accgagctgc
     2881 tgggagatgt t
//
LOCUS       DASYCU010000359         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_224049, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000359 DASYCU010000000
VERSION     DASYCU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224049"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGM24_07305"
     CDS             complement(<1..383)
                     /locus_tag="VGM24_07305"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY4155216.1"
                     /translation="MARRHNVEMAFLPFIRLEGIASKDFRRQKVEIGNYSAVVFTSRH
                     AIDHFFRTAEEMKITVSQDTKYFCVTEAVALYLQKFILYRKRKVFYGADGTNKSMLEV
                     INKHKENEKFLYPTSENQQDNEIVN"
     gene            complement(523..1335)
                     /locus_tag="VGM24_07310"
     CDS             complement(523..1335)
                     /locus_tag="VGM24_07310"
                     /inference="COORDINATES: protein motif:HMM:NF025459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HEY4155217.1"
                     /translation="MNAPVFTDSLRFSNHKPALTSLPSGWMDLLKSNDYLNFSGEPVR
                     NRDELYESRSSDGLFYLLVGLLFFFAIIKLAFGKYLNNLLTLFFRVSMRQQQIREQVI
                     QSPLPSLLLNLLFVLSGGLYACYILQYYHYIAPDQFWVCFLYSSLLLALIYLVKFIFL
                     KISGWIFNIKRTAETYLFIVFMTNKVIGIFLLPFLIIMSFSSNLISTICITGSAILIA
                     VFYFYRLIASYKSLRKEINITALHFFLYLCAFEIAPLLLIYKVLLTYLKKAI"
     gene            complement(1644..>2702)
                     /locus_tag="VGM24_07315"
     CDS             complement(1644..>2702)
                     /locus_tag="VGM24_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155218.1"
                     /translation="KPKRSSQIDVLVGFLPNNDQLTSNKLLVTGEATANLKNPFGNGE
                     SLGLSWQQLQVKSPRLNLYFQQPYLFHSPYGINAGFSLYKKDSSYVTTQGTLGIQFAS
                     SANRTGTVFIQATGSNLLNVDTLQVISTRRLPAIADVSSTSLGASFEINTTNYRFNPV
                     RGNEFLFTGSVGTKKIRKNNQIGKLVDPDDPEFDYGSLYDTIKLSSYQFRLNLAAAHY
                     FGITRASTIRFGINAGWYQSANTFRNELFQIGGYKLLRGFDEESILATQYTAGSLEYR
                     YLIGMNSFLFAFFDGGWAKNDVPGYQLNSIYLGVGLGLAFETKAGIFNISYAIGKQDS
                     NPFAVRQAKIHLGYLNFF"
BASE COUNT          767 a          556 c          662 g          717 t
ORIGIN      
        1 cagttcacaa tttcattatc ctgttggttt tcggaggtcg gatagaggaa tttctcattt
       61 tccttatgct tgttgatgac ttcaagcata ctcttgttgg ttccgtctgc tccataaaag
      121 acctttcgtt ttctatacag gataaatttc tggaggtata gggcaaccgc ttccgttaca
      181 caaaagtatt tggtgtcctg ggaaacggta atcttcattt cttccgcggt cctgaaaaaa
      241 tgatcgatgg cgtgccggct ggtaaatacg acggccgaat aatttccaat ttcaaccttc
      301 tgtcgtctga agtcttttga agcaattcct tcgaggcgga taaatggcaa aaaagccatt
      361 tccacattat gccgcctggc caaatcaaaa taaggtgatt tgtctgattc aggccggggt
      421 tgggtgatta aaattttttt taaagcaatt tctgacatct cggcaaaatc ctttttttcc
      481 ctatttttaa tcatgtaatt attaaaaagc gcgccttcaa aattaaatag cttttttcaa
      541 ataagtaagt aacactttgt aaatcaatag cagaggtgct atttcaaagg cgcaaaggta
      601 aagaaaaaag tgcaatgcgg tgatatttat ttcctttcgg agcgatttat aagaggcgat
      661 cagccggtaa aaataaaaga cggcgatcag gatggccgat ccggttatac agatggtaga
      721 tatcaaattg cttgagaatg acataattat aagaaagggg agcaaaaaga tgccaatcac
      781 cttattggtc atgaagacaa taaacaggta agtttccgcc gttcgtttaa tgttgaatat
      841 ccagccactg attttgagaa agatgaattt tacgaggtaa atcagggcca gcaaaagaga
      901 ggagtacaga aaacagaccc agaattgatc cggagcgata taatgataat attgtaatat
      961 gtagcaggcg tatagtccgc cggacaatac aaagagcagg tttaacaata aagaaggcag
     1021 gggggactga atcacctgtt cccggatctg ctgctgtctc atggaaacac ggaaaaacaa
     1081 ggtgagcaga ttgttcaggt atttgccgaa tgccagtttg ataatggcaa agaaaaagag
     1141 caggcccacc agtaaataga atagcccgtc ggaagacctg gattcatata attcatccct
     1201 gttccgtacc ggttcgccgg aaaaatttaa ataatcattt gatttcagca ggtccatcca
     1261 tccggaagga aggctggtta gtgcaggttt gtggttactg aaacggagag agtccgtaaa
     1321 gacaggtgca ttcattccag gaagggaatc gggcttatgg tgaatcgccg aatcggccgg
     1381 agttttcagg attttcttcc ggggtgccgg aacgggcggc aggctatcgg gtgaagcctg
     1441 cctgatgatc gcagtatctc gggaaccagt ggaatcagcc ggtatttgcg cggaagatct
     1501 gccagccagc ataaaaaaaa agaaaagaag cgggtaaaat tttgccaatg gatatatggt
     1561 tgattgaaga attccggctc ctcccgcatc cgcaacaaca aaatccggag gaattgcaaa
     1621 gttggtattt gaacgcagat cttttaaaaa aagtttaaat aacccaaatg gatttttgcc
     1681 tgcctgacgg cgaacgggtt gctgtcctgt ttcccgatgg cataagaaat attaaaaatt
     1741 cccgccttgg tttcgaaggc cagacccaga cctactccaa gataaatgct gttcagctga
     1801 tagcccggaa catcattttt tgcccagcct ccgtcaaaaa aagcaaaaag aaaggagttc
     1861 attccgatca gatagcggta ttccaggctt ccggccgtat actgggtggc cagaatgctt
     1921 tcttcatcaa agccacgcag taatttatat ccgccgatct gaaacagctc gttgcggaaa
     1981 gtattggcgc tctggtacca tcccgcgttg attccaaacc ggatggtgga ggcccgggtg
     2041 atgccgaaat aatgagctgc agccaggttc agccggaact ggtaggaact gagtttgatg
     2101 gtatcgtata atgatccgta atcgaattca gggtcgtccg gatctaccag ttttccgatc
     2161 tgattattct tccggatctt ttttgttccg accgagccgg taaagaggaa ttcatttccc
     2221 cgcaccgggt taaaccggta attggtggtg ttgatctcga aagaagctcc caggctggtg
     2281 gaactcacat ctgctattgc cgggaggcgc cgggtggaga tcacctgtaa ggtatccaca
     2341 ttcaacagat tggagccggt ggcctggata aataccgttc cggtgcggtt cgccgaggag
     2401 gcaaactgaa ttcccagggt gccctgcgtg gtaacatagg aagagtcttt tttatacagg
     2461 gagaaaccgg cgtttatgcc ataggggcta tgaaaaagat agggttgctg aaaataaaga
     2521 ttcagcctgg gcgactttac ctgcaattgc tgccagctca aaccgaggct ttctccgttt
     2581 ccaaatggat ttttgagatt ggccgtggct tcacccgtta ccagcaattt attgctcgtg
     2641 agctgatcat tgtttggcag aaatccgaca agcacatcta tctgactgct ccgcttgggt
     2701 tt
//
LOCUS       DASYCU010000360         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4283669, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000360 DASYCU010000000
VERSION     DASYCU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4283669"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="VGM24_07320"
     CDS             complement(<1..1961)
                     /locus_tag="VGM24_07320"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4155219.1"
                     /translation="MRKKLLTLGLSTVFAIMGMAQAPAKPAGPAARAGGPPSIGRAFG
                     KVTDSTGQPMEATALVLKSTVDASTKKKKLVLLKGMDTKGNGEFNFEDLPISIPLVLR
                     TSATGYKSEDLSFTITNSAAAPKPGGSSSPYGNLPSFDKDLGSIKLKADVKMLNSVVV
                     TSSTPTMKLELDKKVFNVEKNIVSAGGTGLDVMRNVPSVNVDIDGNVTLRGSKPTIFV
                     DGRPTTLTLDEIPADAIESVEVMTNPSAKYDASGGGAGILNIVLKKNKKKGYNGSITA
                     GMDSHGAPNALAGVNVRQGKINVTANGMINGMNGKTTGNTERFTYLGDTTRLLQQDLN
                     RNKGHFMFGQIGLDYFVSNKTTLSASYIKVHGKFKPTDVSQIQTDSLSYLGNTTGFSQ
                     RNSNSVREFDVNGVQLGMKHIFDKEGEQITADGSYFGVNAHSTSLYTTNYYSGQPGSA
                     VSGITRQQMVGTADPAFFTVQTDYTDPVGKSKLEAGLRASVQKLGNKNDNFTLDKNGD
                     AELNAFGSSNYRSSSQVYAGYVNFTSAVGKFGYSLGLRAESSSYSGDLLNTGQHFSNT
                     YPVSIFPTLFLSQKLENDQELQFSATRKINRPGFFQLIPFTDYSDTLNITRGNPDLVP
                     EFTESFEMTYMKSFQQNHTILFSGYYKYT"
     gene            complement(2086..2973)
                     /locus_tag="VGM24_07325"
     CDS             complement(2086..2973)
                     /locus_tag="VGM24_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155220.1"
                     /translation="MLKSRYNIMQVTMVVFFGVFFMLVPFLLHKDSTHSAGSTILMFI
                     VPLLGASLPYLFFQKAPRVVFDPEFITARYVTGTKTRNWSSVTDIFLSRKEGFLGQSL
                     EATVVVFDNREKLILWQDLYRNLTQMRGFLAQKAAGKIRDAVPDIKEKNLAVISRRRY
                     AGNAYTSFNTLLIVGMIVFFIIAMKHPKAESILFIPAGFVLLLFFGVGTQMNYFVIDE
                     GYLIVRNHYFPWKNKRIDLRQIKEVDVETPNKRSTGLRVLSSDFSSRLYGAGTLRSNN
                     WTELLNDLKLIGIPVRNDG"
     gene            complement(2981..4438)
                     /locus_tag="VGM24_07330"
     CDS             complement(2981..4438)
                     /locus_tag="VGM24_07330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY4155221.1"
                     /translation="MAIGLIPRYSVVFPVEGMTTNQFLMLASRAAKKLNWEISFTGND
                     GFVAYTPFRKRSLNEKIQLKIDAGLVNLQSESTGGQFIDWGWNKRNIDAFILNYDELR
                     SSVTAEELNAVPGESPQAQENENGPAGVSIESTQNSKGFFSLLIPVKGYGVTPVILWL
                     NILVFILMVISGVDFLHPNTQSLLQWGANFRPYTMQGQSWRLFTNFFLHIGILHLLFN
                     MYALLYIGILLEPRLGSWRFGMAYIVTGLMASMTSLYWHPFTVSAGASGAIFGMYGVF
                     LAMLATNLIEKSLRKPLLTSIAIFVGYNLLSGMKGGIDNAAHLGGLISGIVIGYSFYP
                     GLSKTQDKKLNYGLPALLIVALFGFSSWEYRKIPNDIVRYDEKMKSFFSMESQALEVF
                     KMPASTPKEQFMSEIKNRGLYYWREDKNLITELDKLDLPEVIHRRNRMLMKYCDLRIA
                     SYELIYRSITDDTHSYNDQIRNYNDSIESLIKTLK"
     gene            4603..4746
                     /locus_tag="VGM24_07335"
     CDS             4603..4746
                     /locus_tag="VGM24_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4155222.1"
                     /translation="MIITDLTLANLSILKTGKAKAIRFSTLEAICKALDCQPGDLLEY
                     GRG"
BASE COUNT         1249 a         1172 c         1152 g         1279 t
ORIGIN      
        1 ttggtgtatt tataatagcc cgaaaacagg atcgtatggt tctgctgaaa gctcttcatg
       61 taagtcattt cgaaggactc cgtaaactcc ggcaccaggt cgggattacc acgggtaata
      121 ttcagcgtat cggaataatc cgtaaacggg atcagctgaa aaaagccggg gcggttgatc
      181 ttgcgcgtgg cgctgaattg cagctcctga tcattctcca gtttctggct gagaaaaagc
      241 gtggggaaga tactcaccgg ataggtattg ctgaaatgct ggcccgtatt gagcaggtcc
      301 ccggaatagc tcgagctttc cgcgcgcagg cccagggaat atccgaattt gccgaccgca
      361 ctggtgaaat tcacatatcc cgcatacacc tggctgctac tccggtaatt gctgctgcca
      421 aaggcattca gctccgcatc tccattcttg tccagggtaa aattatcatt cttgttgccc
      481 agtttctgaa cgctggcccg cagaccggct tccagttttg atttaccgac gggatcggta
      541 taatccgttt gcacggtaaa gaaggccgga tccgcggtac ccaccatctg ttgccgggtg
      601 ataccggaaa cagccgatcc gggttgaccc gaataataat tggtggtata cagggaagtg
      661 gaatgcgcat ttacgccgaa ataagaacca tcggccgtga tctgctcgcc ttctttgtca
      721 aaaatatgtt tcatgccaag ctgcacgccg ttcacatcga attcccgcac actattggaa
      781 ttgcgttggg agaagccggt tgtatttccg aggtaactca ggctatcggt ctggatctgg
      841 gaaacgtcgg tcggtttaaa ttttccgtgc accttaatat aggaggcgga caaagttgtt
      901 ttattgctga caaaataatc cagcccgatc tgtccgaaca tgaaatgccc tttattccgg
      961 ttcaggtcct gctgcaacag gcgggtcgta tcaccgagat aggtgaaccg ttcggtgttc
     1021 ccggtcgttt tcccattcat cccattgatc atgccattgg cggtcacatt tatttttccc
     1081 tgccgcacgt tgaccccggc cagggcattg ggcgcgccat gggaatccat tccggccgtg
     1141 atgcttccgt tatagccctt cttcttgttc tttttgagca cgatattaag aatgcccgct
     1201 ccaccgccgg aggcatcgta tttggcggaa ggattggtca tcacttccac actctcaatg
     1261 gcgtctgccg gaatttcatc cagggtgagc gtggtcggtc ttccatctac aaatatcgtt
     1321 ggcttcgatc cccggagcgt gacgtttccg tcaatgtcca catttacgga aggcacattg
     1381 cgcataacgt ccagcccggt tcccccggcg ctcacaatat tcttctccac attgaacact
     1441 tttttatcga gctccagttt catggtgggc gtggaggagg taacgaccac ggagttaagc
     1501 atcttcacat cggccttcag tttaatggac cccaggtcct tgtcgaacga aggcaggttg
     1561 ccataagggg aagaactacc gcccggtttg ggtgcggccg ccgaattggt aatagtgaac
     1621 gaaaggtcct cgcttttata accggtggcg gaagtccgca gaacgagggg aatggaaatg
     1681 ggcaggtcct caaagttgaa ttccccgttt cccttggtgt ccattccttt caggaggacc
     1741 agtttctttt ttttggttga cgcatccacg gttgatttca agaccagggc ggtggcttcc
     1801 atgggctgac cggttgaatc agtcactttt ccaaaagcgc ggccgatgga tgggggaccg
     1861 ccggctcttg cggccggacc ggctggttta gcaggagcct gtgccatacc catgattgca
     1921 aagacagtag acagacccag agttaatagt ttctttctca cagtatttta ttttgacagc
     1981 aaaagtaggg ggacaggcta tatgcgacca gtgaataacc gccaaacggg gaaaacttcc
     2041 ggttaccggc gggaaatatt cggttaaaga ggaaaagcgg cccggttatc catcattccg
     2101 gacgggtatg ccgataagtt taagatcatt tagaagctcc gtccagttat tgcttctcag
     2161 ggtccccgct ccgtacagcc tggaactgaa gtctgatgac aaaacccgca atccggtcga
     2221 ccgcttattc ggagtctcaa catcaacttc cttgatttgg cggaggtcaa tccgtttatt
     2281 cttccagggg aaataatgat tcctcacaat taaatatcct tcatcaatta caaaataatt
     2341 catctgggtt cccaccccaa aaaacaatag cagaacaaat cccgccggaa taaaaagtat
     2401 ggattcggct tttgggtgct tcatagctat aataaaaaat acaatcatac cgacgatcaa
     2461 taacgtattg aatgaagtgt atgcgtttcc ggcatatctc cttctcgaga tgacagccag
     2521 gtttttttcc ttaatgtcag gaaccgcgtc ccggatcttt cccgctgctt tctgagcgag
     2581 gaaacctctc atctgggtaa ggtttctgta caaatcctgc cacaggatca atttctcccg
     2641 gttgtcgaaa acaacgactg ttgcttccag ggactgaccc agaaatcctt ctttccggct
     2701 caggaaaata tcggttacag acgaccagtt ccgggttttg gttccggtta cataccgggc
     2761 ggttataaat tccgggtcaa atacaaccct cggggctttt tgaaagaata aataaggcag
     2821 ggatgcgccc agcaggggaa cgataaacat caaaatagtt gatccggcgc tatgcgtcga
     2881 atccttatgt aataaaaacg gcaccagcat aaaaaatacg ccgaaaaaaa caaccatcgt
     2941 cacctgcata atattatatc tcgattttag catagtctga ttatttcaac gtcttaatta
     3001 atgattcgat gctgtcattg taattcctga tctgatcatt gtatgagtgg gtatcatctg
     3061 ttatggatct ataaatcaat tcataactgg caatccggag atcacaatat ttcattagca
     3121 ttctgttcct ccggtggatt acttccggca ggtcgagttt gtcgagttcc gtgatcaggt
     3181 ttttatcttc cctccagtag tacagtcccc ggtttttgat ttcagacatg aactgttctt
     3241 ttggagtgga agccggcatc ttaaatacct ccagcgcctg ggattccatg ctgaaaaaag
     3301 atttcatctt ctcatcatac cgcacgatgt cattgggaat cttcctgtat tcccaggagg
     3361 aaaatccaaa aagagcaacg ataagcaatg ccggcaagcc ataattcagc tttttatcct
     3421 gtgtttttga aaggccggga taaaaggaat acccgatcac tatgccggaa ataagaccac
     3481 ccaggtgagc tgcattgtca attcctcctt tcattccgct caaaaggtta tagccgacaa
     3541 aaatggcgat gcttgtcagt aatggctttc tcagggattt ttcaatcagg ttcgtggcaa
     3601 gcatcgcaag gaatacgccg tacataccga atatggcgcc ggatgctccg gcactgacgg
     3661 tgaacggatg ccaataaagg ctggtcatgc ttgccattaa tccggttacg atataagcca
     3721 tcccaaatct ccagcttccc agtctcggct ccagcaggat cccgatatac aggagcgcat
     3781 acatattgaa aagcaggtgc agtatcccga tgtgaagaaa aaaattcgta aacagccgcc
     3841 agctctgccc ctgcatggta taaggccgga aattagcacc ccattgaagc aggctttgtg
     3901 tattcgggtg caggaaatcc acgccggaaa tgaccatcaa tataaagacg agaatattga
     3961 gccaaagtat gaccggggtc actccatatc ctttgacagg tatcagtagg gaaaaaaagc
     4021 cttttgaatt ttgggtagac tctatgctca ctcccgcagg tccattttca ttctcctggg
     4081 cttgaggaga ttctccgggc actgcattta attcttccgc ggtaacagac gatcttaatt
     4141 catcataatt caaaataaac gcatcgatat tgcgcttgtt ccatccccag tcaatgaatt
     4201 gtccgcccgt gctctcactc tgcaaattga ccaggccggc atcaatcttc agttggatct
     4261 tttcattgag cgagcgtttc cggaacggcg tataggcaac aaagccatcg ttcccggtga
     4321 aagagatttc ccagttcagc ttttttgccg cccgggatgc cagcattaaa aactggttcg
     4381 ttgtcattcc ttctaccgga aatacaacgg agtaacgggg tatcaatcct atggccattg
     4441 caggggttat tcggggttaa aattgaatat agaacgaatc agagagggat atcatatttg
     4501 aattgaaatt tcgccggaaa atttagagat ccgggttttt aaaacaggta gggggtgaga
     4561 gataggaaaa atgagaattg ggaagtcctt gtttatgagg acattataat tacggatctg
     4621 acgctggcca atctctccat actcaaaacc ggaaaggcaa aagccatccg gttcagcacc
     4681 ctggaagcca tctgcaaggc actggactgt cagcccggag atttattgga gtatggcagg
     4741 gggtaatcat aaaaaatcaa ataccggaca tgacccttcg gactactccg gcatcagctt
     4801 ctcaatatcg tcaagatcct tgtgacgtcc cgatgtcttt ttggcctgaa tc
//
LOCUS       DASYCU010000361         6129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1237765, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000361 DASYCU010000000
VERSION     DASYCU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237765"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            500..1231
                     /locus_tag="VGM24_07340"
     CDS             500..1231
                     /locus_tag="VGM24_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY4155223.1"
                     /translation="MKVQIDHHSKLPLHVQVEELLRKMAAAPEFRGGAFLPKEVELAN
                     RLGVSRNTIRQATNKLQNEGLLTRKKGVGTKVAKKKPLATGLDHWYSFTQEMREKGIQ
                     VVNLELRIAWVSIDKKIADFFNIPGNTKVLKLSKLKGTAGDPIVYFESYFHPRTGVTE
                     NDEFDIPLYTMLEKRYGVLVARSSENISARLAGEVGKKLKIRASEPVLFRERFVYDAG
                     DRPVEYNLGYYRSDKFIYSIDIKKA"
     gene            1254..3971
                     /locus_tag="VGM24_07345"
     CDS             1254..3971
                     /locus_tag="VGM24_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY4155224.1"
                     /translation="MCKHRDKVILGVDIGGTHISSALVSAADGCVLEETFCKKEVDTR
                     ASGPNGILGRWMQALQQTISGLGEAELKGIGIAMPGPFDYENGISLLQGVDKYESLFG
                     VNIRSAMESHLIPGLRVPVIFENDSACFGLGEGLTGEGASHKNIIAITLGTGFGASFI
                     RQGKIIKQGPGVPDQGYLYNIPFKNGIAEDYISARWLLNSYFELSGRRLHAVKELAEL
                     AETEKDPAAKEVFRTYGNNLAACLAHWLRSFKANCLIIGGSISNAMPLFLPELIRALK
                     ENEGINIPIKRSKKTERSAISGAAGLVEIADKMMENKRKQAVKWRKSSQKILPQTAEG
                     ISRKAGEYDMYPFYSLGKDKIFSGYQALAEWIAEEKRVAIDGYVGVDWPMIRERLSTA
                     FAKMNLKVLWYETSAFCKEEREIGKLVRPFLGEEDSVWGKKTTLALEDFYDLEKLEQL
                     QPDKNFDVTILLGTGAGLCNENMPLIYVDLPKNEIQYRMRAGSINNLGTTRSTSASAM
                     YKRFYFVDWVLLNKYRQKIKHKIAVVADGQRKDSISWARYDSIAEGFRFMSKNVIRAR
                     PWFEAGAWGGQWMKEHIPSLNRNEINYAWSFELIVPENGLVFESNGHLLELAFDWLME
                     SCSKEVLGEDAKRFGTAFPIRFDFLDTFDGGNLSIQCHPSLPYIRKHFGETITQDETY
                     YILDCKKGAGVYLGFQPDINAEQFREVLEESEEKDTPVEIEKYVQWHPSHKHDLFLIP
                     NGTIHSSGVNNLVLEISATPYIFTFKMYDWVRLDLEGNPRPINIEHAFNNLDFGRKGD
                     VVKEELISRPGLIEETDHYKLIHLPTHKEHFYDVHRIDFLKEAEMKTNGKCHVLMLVE
                     GVSLTVETADGYRQRFNYAETFIIPAAAGSYRLINEEGGLIRVIKAFIK"
     gene            3997..5424
                     /locus_tag="VGM24_07350"
     CDS             3997..5424
                     /locus_tag="VGM24_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY4155225.1"
                     /translation="MNDKQTIPNKRYFLLVCFIAALGGFLFGFDTAVISGVLEFVVHD
                     FRFNLFMEGWFVSSALLGCILGVAAAGKLSDWYGRKKVMLLSACLFFLSALGCMLATG
                     SALLISFRLIGGLGIGVASMICPLYIAEFSPAHFRGRMVALYQLAITLGIVAAYFTNA
                     YLVKISLQHAFSGWLHYIITQDVWRSMIGIGMVPAFIFLVCLFFVPESPRWLLLKGRE
                     KEALGYLEKLNPPAAAQTALNEIRENIKTAGGSIRELVSPVYRRALIIGLALPFLSQV
                     SGINAIIYFGPTILDKAGFSLGDALGGQVTIGIVNVLFTFVAIFTVDRWGRKPLLILG
                     ISGAVLSLLVLGVLFKMQVTEGYWILIFILLFIACFAFSFGPVCWIIISEIFPNALRG
                     RAMSLATLSLWVANFFVGQLTPLMLKSIHWGPAATFWTFAVLCAPGLWLTWKWIPETK
                     GKSLEEIEDSWKAKRSPVIRKEGAL"
     gene            5550..>6129
                     /locus_tag="VGM24_07355"
     CDS             5550..>6129
                     /locus_tag="VGM24_07355"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4155226.1"
                     /translation="MRLFKSERKSFISFKKLLLFFGFTVFFSGLFYAASAQKLNTVTG
                     VVQDTAGTPVSGVSVFLKGSKKGVITDDNGKYSLRMSRAQANDTIVFSNVGYVLQKHA
                     VPVSGVLDVQLTAKASQLSDVVVVGYGAQKKGALTSAVSSVQGPEIVTTKNENILNTL
                     TGKVAGLRIVQNTSEPGSFNNSFDIRGMGSPLI"
BASE COUNT         1545 a         1408 c         1653 g         1523 t
ORIGIN      
        1 tcaagtgaga tcagagcggt ttcaaagcgg tttcaaagcg gtttcaaagc ggtttcaaag
       61 cggtttcaaa gcggtatcaa agcgatggat aagaataggc cggaagcggc ctgtttccat
      121 ctccactctg ccagattttc atatttaatc agcccgttcc agaccaaatg ctttcttcat
      181 tggggccggg ggaaatcctt tttcaattta ccggagccct gaccgccaac agatggtttc
      241 ccgccggcgc ggatttattt cggagaactg ccctggaaaa ccgggcatcc aacatgcctt
      301 tccggatcag cccgtaaaaa tatcggatgg ataaatgtat ggaagatttt tcttgcgccc
      361 cttacattta aggagcggtt gggccgccgg tcaatgtaga atgaatccgg gctgcagtgc
      421 gggtccctca aattatattc ctccaaattt ttatttattt aataaaatcc agtatgtttg
      481 aacattcgta caataaaaaa tgaaggtaca gattgaccac cacagtaaac tgccgctgca
      541 cgtacaggta gaggaattgc ttcggaaaat ggctgctgcg cccgaattca ggggcggggc
      601 ttttttgccg aaagaagtgg agttggccaa ccgcctgggc gtttcccgca acaccatccg
      661 gcaggcgacg aacaagctgc agaacgaagg gctgttgacg agaaaaaaag gagtggggac
      721 caaagtggca aagaaaaagc cgctggccac cggcctggat cactggtata gcttcaccca
      781 ggagatgcgc gagaagggga ttcaggtggt gaacctggag cttagaatcg cctgggtctc
      841 catagacaaa aagatcgcgg attttttcaa tattccgggg aacacgaagg tcctgaagtt
      901 gtcaaagctg aaaggcaccg ccggggatcc gattgtttat tttgaaagct attttcaccc
      961 ccgcaccggc gtcaccgaaa atgatgaatt cgatattccc ctgtacacca tgctggaaaa
     1021 gcggtacggc gtcctggtcg cgcggtcgtc cgagaatatc agcgcccggc tggcgggaga
     1081 ggtggggaaa aagcttaaga tacgggcatc cgaacccgtg cttttcaggg aaagatttgt
     1141 ttacgatgcg ggggaccggc cggtcgaata caacctcggc tattaccggt ccgataagtt
     1201 catttattcg atagatatca aaaaggcctg agtttttttt catgagcgtg aatatgtgca
     1261 aacataggga caaagtgatc ctgggggtgg atatcggagg aacccatatt tcttctgcgc
     1321 tggtcagcgc ggcggatggc tgcgttctgg aggaaacttt ctgtaaaaag gaggtggaca
     1381 ccagggcatc gggtccgaac gggattcttg gaagatggat gcaggcgctt cagcaaacca
     1441 tttccgggtt gggagaggct gaattaaaag gaattggtat tgcgatgccc ggaccatttg
     1501 actacgagaa tggaatttcg cttctccagg gggtcgataa atatgaatcc ctgtttggcg
     1561 tgaatatcag gtccgcgatg gaaagccatc tgatccctgg tctccgggtt ccggtgattt
     1621 ttgaaaacga cagtgcctgt ttcggtttgg gggaaggact gacgggagaa ggcgcttccc
     1681 ataagaatat aattgccatc acgctgggga cgggctttgg cgcttccttt atccgccagg
     1741 ggaagatcat aaaacagggt ccgggtgttc cggaccaggg ttacctgtat aatattcctt
     1801 tcaaaaacgg gatcgctgaa gattatattt cggcacgctg gctcttaaac agttatttcg
     1861 agctgtcggg tagaagactc catgcagtaa aggaattggc tgagctggcg gagacagaaa
     1921 aggatcccgc agcaaaagaa gtttttagaa cctatggcaa taatctcgcg gcctgtctcg
     1981 cccactggct ccgttcgttt aaagcgaatt gcctgatcat cgggggaagc atcagcaatg
     2041 caatgcccct gtttttgccg gaactgatca gggcactaaa ggaaaatgaa ggcataaata
     2101 ttccaataaa gcgctccaaa aaaacggaac ggtcggccat ttccggagcg gccggcctgg
     2161 ttgaaatcgc agataaaatg atggagaata aaagaaagca ggcggtcaag tggcgtaagt
     2221 cttcccagaa aatattgccg cagacggcgg agggcatttc ccgcaaagcg ggggaatatg
     2281 atatgtatcc tttttacagt ctgggtaagg ataaaatatt ttccgggtac caggccctgg
     2341 cagaatggat tgcggaggaa aaaagagtag cgatagacgg ttatgtgggg gttgactggc
     2401 ccatgatccg ggagcggctt tccactgctt ttgccaaaat gaatttgaag gttttgtggt
     2461 atgaaacctc cgccttctgc aaagaggagc gcgagatcgg gaaattggtc cggccttttt
     2521 tgggagaaga ggattcggtc tggggtaaaa agacgaccct cgccctggag gatttctatg
     2581 accttgaaaa actggaacag ctgcagccgg acaaaaattt tgatgtgacc atcctgctgg
     2641 gaaccggcgc aggcctgtgt aatgaaaata tgccgctgat ctatgtagac cttcccaaaa
     2701 acgaaatcca gtaccgcatg cgcgcgggaa gcatcaataa tctcgggacc acccgcagta
     2761 cttccgcatc ggccatgtac aagcggtttt actttgttga ctgggtgctg ctcaataaat
     2821 accggcaaaa aataaaacat aagattgcgg tcgttgctga cggccagcga aaagatagta
     2881 tcagctgggc gcgctatgat tccattgcgg aagggttccg gttcatgagt aaaaatgtga
     2941 tccgggcaag gccctggttt gaagccggcg cctggggcgg acagtggatg aaggaacata
     3001 ttccttcctt gaaccggaac gagatcaact atgcctggtc atttgaattg attgttccgg
     3061 aaaacggatt ggtcttcgaa agcaacgggc atttgttgga actggctttt gactggttga
     3121 tggaatcctg ttcaaaagaa gtattgggtg aagacgcaaa acggttcggg accgcttttc
     3181 ccatccggtt cgatttcctg gacacgtttg acggcggtaa tctttccatt caatgtcatc
     3241 cttcgctgcc ctatatccgg aagcatttcg gggaaaccat cacccaggat gagacctact
     3301 atatcctgga ttgtaaaaaa ggcgccggcg tttatctcgg cttccagccg gatattaatg
     3361 cggaacagtt ccgcgaagtg ctggaagaaa gcgaggaaaa agatacgcct gtcgagattg
     3421 aaaaatatgt tcaatggcat ccttcccaca aacacgatct ctttctcatc cctaacggaa
     3481 ccattcacag ctcgggtgtg aacaatcttg ttttggagat cagcgccacg ccctacattt
     3541 tcacgtttaa aatgtacgac tgggtaaggc tcgacctgga agggaatccc cgtcccatca
     3601 atattgaaca cgcttttaat aatctcgatt tcggccgcaa aggggacgtg gtaaaagaag
     3661 aactcatttc ccggccagga ctgattgagg aaacggacca ctataagctg atccatttac
     3721 ccacccacaa agaacatttt tatgacgtgc accggattga tttccttaaa gaagcggaaa
     3781 tgaaaacgaa cggcaaatgt catgtcctga tgctggtgga gggcgtttcc ttaacggtgg
     3841 aaacagcgga cggctaccgg caacggttca attacgctga aacatttatc attccggcag
     3901 cagccggatc ttaccgctta ataaatgaag aaggcggttt gattagggtg atcaaggcat
     3961 ttataaaata gaacgaaaag gagagccagt aaaatcatga atgacaaaca aaccatacca
     4021 aacaaaagat atttcctgct ggtgtgcttt atagctgccc tgggaggttt tctttttggt
     4081 ttcgatacgg cggtgatatc cggcgtcctg gagtttgtcg tgcatgattt ccgcttcaac
     4141 ttattcatgg aaggctggtt tgtgagctcg gcattattgg gatgcatact gggtgtggcc
     4201 gcggccggga aactgagtga ctggtatgga agaaagaaag tcatgctttt gtcggcctgc
     4261 ctgttcttcc tgtctgccct cggctgcatg ctggctaccg gttcagccct gctgatctcc
     4321 ttccgcctga tcgggggttt gggcattggc gtcgcttcga tgatctgccc cctgtacatc
     4381 gcggaatttt ctcccgcaca tttcaggggc cggatggtcg ccctgtacca actggccata
     4441 acgcttggga tcgtagcggc ttatttcacc aatgcgtacc tggtaaaaat atcattacaa
     4501 catgcgttca gcggctggct gcactatatc ataacccagg atgtctggcg gtctatgatc
     4561 ggtatcggca tggtccctgc ttttatcttc ctggtatgcc tgttttttgt tcccgaatcg
     4621 ccccggtggt tattattgaa aggcagggag aaagaggcgc ttggctatct cgaaaagctg
     4681 aacccacccg ctgcggcaca gactgcccta aatgagatca gggaaaatat caaaacggca
     4741 ggcgggagta tcagggaact ggtctccccg gtttaccgcc gggccctcat catcggcctt
     4801 gcgctgccct ttctttccca ggtctccggc attaacgcga tcatttattt tgggccgacg
     4861 atcctggata aggccggctt ttccctcggc gatgccctgg gcgggcaggt gaccatcgga
     4921 attgtgaacg tgctcttcac cttcgtcgcc atttttacgg tcgaccgctg gggcaggaaa
     4981 ccactcttga tcctgggtat ttccggcgcc gtactatcgc tcctggtttt gggcgtatta
     5041 tttaaaatgc aggttacgga gggatactgg atactgatct ttattttatt gttcattgcc
     5101 tgttttgcct tttcgtttgg acctgtgtgc tggatcatca tcagcgagat ctttccaaat
     5161 gcgctccggg gaagggcgat gtcgctggcc accctgtctc tctgggtcgc gaattttttt
     5221 gtcggccagc tcaccccgct gatgttaaaa agcattcact ggggccccgc agcgaccttt
     5281 tggacatttg ccgttttgtg tgcgccggga ttatggctta cctggaaatg gataccggaa
     5341 acgaagggaa aatcgctgga agaaatagaa gactcctgga aagcgaagcg atccccggtt
     5401 atccggaagg agggagccct ataaaagttc atctaaaagg agagaaccga tgttcactaa
     5461 tatgttcaga caatcagaca tatacggccc gatcttcccg ctttcccggt atccggtcac
     5521 acattcacta acgtttaaac ttttgccaaa tgagactatt taaatcagaa cggaagtcct
     5581 ttatttcctt taaaaagctg ctcctttttt tcgggttcac ggtctttttc tccggtttgt
     5641 tttatgctgc gtcggcccag aagttaaata ccgtcaccgg tgtggtgcag gatacggccg
     5701 gaacaccggt atccggcgtt agtgtttttc tcaaagggtc caaaaaggga gtgatcacgg
     5761 atgataacgg aaaatacagc ctccggatgt cccgggcaca ggcaaacgat acgatcgtat
     5821 tttccaatgt cgggtatgtt ctgcaaaaac acgcggttcc cgtttccggc gttctggatg
     5881 tgcagttgac agccaaagca agtcagctta gcgacgtggt ggtggttggt tacggtgcgc
     5941 agaagaaagg agcgcttacg agtgcggtat cgtcggtgca gggccccgaa attgttacca
     6001 caaaaaatga aaatatactg aacacgctga ccggcaaagt ggccggcttg cgaatagtgc
     6061 aaaataccag cgaaccggga agcttcaaca attctttcga tatccggggt atgggttcgc
     6121 cgttgatcg
//
LOCUS       DASYCU010000362         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1237858, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000362 DASYCU010000000
VERSION     DASYCU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237858"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VGM24_07360"
     CDS             <1..997
                     /locus_tag="VGM24_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6786 family protein"
                     /protein_id="HEY4155227.1"
                     /translation="GEGGQSFGWINYKAFAGEPNAHMNAYGGENRIWLGPEGGKYSLF
                     FSKGAKMDFDHWKTPASFDTEAWDLVSQSKQEVSLHKKMTLQNYAGSVLQVDADRRIS
                     ILSRDRIDSILHLDPDDSVKAVGYETQNKITNSGKTAWDEKTGMPCIWMLDMFNPSEK
                     TVILIPYQRPSDTVSKIATTNYFGEIPADRLKHANGILYFRADGKSRGKLGLDPSRAT
                     RYAGSYDPAHHILTVAYFDLDPSAKYLNQEWNTARPVFSGDAVNAYNDGPLADGTQMG
                     PFYEMESVSPAAQLKPGESLEHHHSIFHFTGPEENLNKITETVFHVSISNIKSVF"
     gene            1230..>2207
                     /locus_tag="VGM24_07365"
     CDS             1230..>2207
                     /locus_tag="VGM24_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4155228.1"
                     /translation="MRRLPDLQLVQRSRLTAPRNGFPVKALLFFFLFLGFSTSGLAVR
                     SSLSRAVIGTVTNAEGSPLPGVTVTLKGSTMAVSTDNTGAFSLDIPEDQANAVLVFSS
                     VGYVTREIAATGNTPLKVAMETDIRNLNDVVVVGYGTQRRKDLTGSIASVSSAQIAQR
                     PVSSYEDALAGVAAGIDVAPRSARPGNTSEIVIRGIGTISGDREPLFVVDGFPTDALN
                     AAAINPDNIASVDILKDASSTAIYGSRGANGVIIITTKSGRAGQARVDVNLKTGFSEA
                     NKHDYYKVLNGEQYVQWYKEKAINDGTPIPSWVTNWDGTSTNWQNVIYRK"
BASE COUNT          558 a          535 c          596 g          518 t
ORIGIN      
        1 aggggaggga ggtcagagtt ttggctggat caactataaa gcgtttgccg gggagccgaa
       61 tgcacatatg aacgcctatg gcggagagaa ccggatctgg cttgggccgg agggtgggaa
      121 atattccctg tttttcagca agggcgcaaa aatggatttt gatcattgga aaacaccggc
      181 ttcattcgat acggaagcct gggatctggt gtcgcaaagc aaacaggagg ttagtctgca
      241 taaaaaaatg accctgcaga attatgccgg atccgtattg caggtggatg cggaccgcag
      301 gatatccatt ctttcgcgcg accgcattga ttccatcctt catctggatc ccgatgattc
      361 cgtgaaagcg gtcgggtatg aaacgcagaa taaaataacc aacagcggga agacagcctg
      421 ggatgaaaaa acaggtatgc cctgcatctg gatgctggac atgttcaatc cgtcggaaaa
      481 aacggttatc ctcatccctt atcagagacc gtcggacacg gtatcaaaaa tcgcaacgac
      541 caattatttc ggggaaatac cggcggaccg gctaaaacac gcgaacggaa ttttatattt
      601 cagggcagat ggaaagagca ggggtaaact ggggcttgat ccttcccggg ccacccgcta
      661 tgcgggaagt tatgatccgg cccatcatat ccttacggtc gcttattttg atctggatcc
      721 ttccgcaaaa tatctgaacc aggaatggaa taccgccagg ccggtatttt cgggcgacgc
      781 cgtaaatgca tacaatgatg gtccgcttgc cgatggaacg caaatgggac ctttctatga
      841 aatggaaagc gtgtctcccg ccgcgcagct gaaaccggga gaatcgctgg agcaccacca
      901 cagtatattt cattttaccg gcccggaaga aaacctgaat aaaatcactg aaactgtttt
      961 tcatgtttcc atcagcaaca tcaaatcggt cttctgaaac aactgccaaa aataaatttt
     1021 gttgctttaa acgtttaagc taggtttggg gagtggttgg ccggtttgcc gttccggtcc
     1081 cttatgcggt cttcccccac gggaggatca tccggtcatt ttggttctct tttttttatc
     1141 cgtactgctt aaacgtttaa atagagcctt ccggtggatt tgcggtgacg ggtcgccggt
     1201 ataaattgtt taacaccaaa attaaatata tgagacgatt gcctgacctg cagcttgtac
     1261 agcgtagccg gcttaccgcg ccccggaacg ggttcccggt gaaagccctg ctttttttct
     1321 ttctttttct gggattcagc acttccgggc ttgcggtccg gagtagtctt tcgcgtgccg
     1381 tcatcggaac ggtaacgaat gcggaaggtt cgccgctgcc gggcgttacg gttacgctca
     1441 agggcagcac gatggcggtc agtaccgata ataccggcgc cttcagcctg gatatccctg
     1501 aggaccaggc caacgcggtg ctggtcttca gttccgtcgg atatgttacc cgggaaatcg
     1561 ccgcaaccgg taacaccccg ctgaaggttg ccatggaaac agatattcgg aacctgaatg
     1621 acgtggtggt ggttggttat ggtacccaga ggagaaaaga ccttaccgga tccattgcct
     1681 cggtctcctc ggcacaaatt gcccaacgcc cggtgagttc ctatgaggat gccctggcgg
     1741 gcgttgctgc cggaatcgat gtggcgccga gaagtgcaag acccggcaat acttccgaga
     1801 tcgtaatacg gggaatcggg acgatttccg gcgaccggga gccactgttc gtggtagacg
     1861 ggtttccaac ggatgcgctc aatgcagccg ccatcaatcc agacaatatc gcttccgtgg
     1921 atatcctgaa agacgcttct tccaccgcaa tctatggttc ccgtggcgca aacggtgtga
     1981 tcattattac gacaaaatcg ggaagggctg gtcaggcaag agtggatgta aacctgaaaa
     2041 caggtttttc cgaagccaat aagcacgatt attataaggt gctgaacggg gagcaatatg
     2101 ttcaatggta taaggaaaaa gccattaacg acggaactcc cattccttcc tgggtgacca
     2161 actgggacgg cacgagcacc aactggcaaa acgtcattta ccggaaa
//
LOCUS       DASYCU010000363         5665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2688770, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000363 DASYCU010000000
VERSION     DASYCU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688770"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="rpsB"
                     /locus_tag="VGM24_07370"
     CDS             complement(<1..724)
                     /gene="rpsB"
                     /locus_tag="VGM24_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY4155229.1"
                     /translation="MEQNTSLQQQLLEAGVHFGHLRKKWNPKMLPYIFAEKKGIHIID
                     LNKTTEALQEASSALKQIAKSGKKIMFVGTKKQAKEIVTESAKKVNMPFVTERWLGGM
                     LTNFNTVRKSVKKMQSIEKMLTDGTFDSITKKERLTLARDKDKMEKVLGGIAQLGRVP
                     AALFLIDIGHEHIALAEARKLGITTFGMVDTNSDPNKVDFAIPSNDDATKSIAIVVNY
                     IAAAIAEGLAERMAAKDDEVEEV"
     gene            complement(786..1175)
                     /gene="rpsI"
                     /locus_tag="VGM24_07375"
     CDS             complement(786..1175)
                     /gene="rpsI"
                     /locus_tag="VGM24_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY4155230.1"
                     /translation="MEKQKTATGRRKEAVTRIFLTRGSGKIVINDKDYKQYFSLAYLQ
                     NEVDLPLKTTDSLDKFDIQINATGGGIKGQAEACKLGIARALLEVNPEFRPVLKAAGV
                     LRRDPRAVERKKPGRKKARRSFQFSKR"
     gene            complement(1257..1715)
                     /gene="rplM"
                     /locus_tag="VGM24_07380"
     CDS             complement(1257..1715)
                     /gene="rplM"
                     /locus_tag="VGM24_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY4155231.1"
                     /translation="MSKLHFTTKHANEATVQRNWYVVDGTNQTVGRMCARIAAILRGK
                     NKAYYTPHVDCGDFVIVTNAEKVLFTGNKLEEKIYINFSGYPGGKKEETARNLQRRRP
                     EAVIERAIKGMLPKNSLGRKMYKKLFVYAGSEHPHIAQQAKPLSFETITR"
     gene            1968..2921
                     /locus_tag="VGM24_07385"
     CDS             1968..2921
                     /locus_tag="VGM24_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155232.1"
                     /translation="MRRKSLLVIIALLIIIAVIAWAPLHFEIKKEIRIETPIEYVGRQ
                     FTDLRHWKNWNVAVRRLDSSGIKYSASSSGINAFLQSGDLLYTIIQTNPAFVLVRSGQ
                     PGTREYHSLYAVSDSFGKSTEVTWTRSLPPARWLKEKIIPDGEMEEGLKNLKNTLEDP
                     LHIYGFPIRIGPVTDTLVITCTATCAKKDKIAELNRLYQGIYGYAKRRKMPVADSRMA
                     NFTFRGKDSLQIDAGIPTSVNGDPENNISVLRMPTRGRMLIGSYEGPYSGLGKLYSAM
                     GKYTRDKRLMKVAAVYEKYLTDPKSATDSLHMKIELCFPVF"
     gene            complement(2918..>5665)
                     /locus_tag="VGM24_07390"
     CDS             complement(2918..>5665)
                     /locus_tag="VGM24_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY4155233.1"
                     /translation="SIHQTANYGDSSVRSKYSAVSGGKLFHHEGNHFRDVTAGSGIIS
                     SSLGYGLGVGVADFNHDGYDDIYVSNDFHENDYYYLNLGNGRFREMNAQAFGHESKYS
                     MGSDIADVNNDGWPDLLTLDMLPEDEKVLKSSATDETYDNYRLKIKAGYHYQYSRNCL
                     QLNVGRGKWFSDIGLYSGIAATDWSWSPLIADFNLDGIDDIFISNGIRNRLNDLDYEK
                     FLSGNQMNIRYNETRSHDREILSQQPPGAVHNYIFEGDSVLRFTDRSADWGFGKADLS
                     QGAAYADLDNDGALDLVTNNMNSEAGIYRNTIRTRSPGSHYLTVQLKGASPNTFAIGA
                     KVFVFYAGKLLYRELQTVKGFMSSVEPLLHFGLNHSAVADSLLVIWPDNTFQRLKKVP
                     ADQKLVIPYDRRKSDTITDERQFINRLLKEPDTSFFSNVPVPLSPDFKDFENPAGIND
                     FNRQPLIPHLLSPLAPKLAVADVNADGLEDFFVCGGKGEPAKLYIQQRDGSFKSGDDS
                     LFGKDAACEKEDALFFDADQDGDPDLYVVNGGNISASKGDLEQDRLYLNDGNGNFTRS
                     AGLPLLSGNRSVVRAADYDGDGYKDLFIGGRANPGNYGTVPQSYLLHNDGRGNFAIVT
                     DAVAPGLKMAGMITDACWTDINRDGRPDLVVVGDWMAPAIFINRNGRLEKEHTSLDGM
                     TGWWTCIKSTDLDGDGYDDLLLGNYGLNSKLKASAEYPVKMYVGDFDANGIPEQLLSI
                     QKDGKYYPFLGKEELEKQLPYLKKEYLSYSKMAGKTMEEIFGEKLRKADLLQASTLAS
                     VVLMNDRKGGFIVKPLPVPMQWAPIFSFYTGDFDHDGKKDLLAAGNFFGTRPFEGRYD
                     AMPPTLGIGDGKGNFTCRIPFADNLLISGEVRDMQTLQVRDQRFLVLARKNEKLIFLS
                     Y"
BASE COUNT         1334 a         1502 c         1316 g         1513 t
ORIGIN      
        1 tcacttcttc tacttcatca tccttggctg ccatcctttc ggcaaggcct tctgcaatag
       61 ctgctgcgat ataatttaca acgatcgcga tggacttggt tgcatcatca ttggaaggga
      121 tcgcaaaatc cactttattg ggatcggaat tggtatccac cataccgaaa gtggttatgc
      181 cgagttttct tgcttccgcc agcgcgatat gctcatgacc gatatcgatc aggaataacg
      241 ccgcgggaac ccgtcccagc tgggcgatac cccccagcac tttctccatt ttatccttat
      301 cccttgccag ggtgagacgt tcttttttag tgatactgtc gaaagtacca tccgtcagca
      361 tcttttcaat gctctgcatt ttcttcacac tcttgcggac ggtattgaaa ttggtgagca
      421 tgcccccgag ccagcgttcg gtaacgaaag gcatattcac tttttttgcc gattccgtta
      481 cgatttcctt cgcctgcttt ttcgtaccga cgaacatgat tttcttaccg gatttggcaa
      541 tttgtttcag cgccgaggaa gcttcctgaa gagcttccgt ggtcttattg aggtcgatga
      601 tgtgaatgcc cttcttttcc gcaaagatat aaggcagcat tttggggttc catttcttac
      661 gcaaatggcc aaagtgcaca ccggcttcca ataactgctg ctgaagtgaa gtattctgtt
      721 ccatattgat taaactttga gccccgggtt gcaggaaccc gggcaggctg tttgaatgat
      781 tgtaattaac gtttgctgaa ctggaagctt ctcctggctt ttttgcggcc cggcttttta
      841 cgttcaacag cccttgggtc gcgacgcagc acaccggccg ctttcagtac ggggcggaac
      901 tcggggttca cttccagtaa ggcccgggcg atgcccagct tgcaggcttc cgcctgaccc
      961 ttgatgcctc cgccggtcgc attgatctgg atatcgaatt tatccagcga atcggtggtc
     1021 tttaacggca gatccacctc attctgcagg taggccaggg aaaaatattg tttataatct
     1081 ttatcgttga tcacgatctt gccgctgccc cgggtcagga agatcctggt tactgcctct
     1141 ttacggcgtc cggtggcggt tttttgtttt tccatgaaac agaaaaattt gaaaatttaa
     1201 taattcgaaa atgatttctc caccgccatt ttagcgaagg gtcaatatca ttcttttcac
     1261 ctggtaatgg tctcgaaaga taagggcttg gcctgctggg caatatgcgg atgctccgat
     1321 ccggcataca caaacagctt tttatacatc tttcttccca ggctgttttt cggcagcatc
     1381 cctttgatgg ctctttcgat caccgcttcc ggccgccgtc tctgcaggtt cctggccgtt
     1441 tcctcttttt taccccccgg atacccggag aaattgatat agatcttttc ttccagttta
     1501 tttccggtaa acaacacctt ttctgcattg gtgacgatca caaaatcgcc gcagtcaacg
     1561 tgtggtgtgt aataggcctt gttcttcccg cgcaggatgg ctgcaatacg ggcacacatg
     1621 cgtccgacgg tttgattagt accgtccaca acgtaccaat tccgttgtac ggtagcctcg
     1681 ttcgcatgtt tggtggtaaa atggagtttg ctcattatgc ttatttttga tttcgcctcc
     1741 gctatcccgg gtggcatatt taaatggacg gcaaaggtag gtgtaacaaa tgcttattcc
     1801 caaaatttga aatggtattt tttataggcc cctttataac tcaatgataa tcaataaaaa
     1861 ttttgactgt tcagataaat gctacattac cggggaattg ctttccattt ggttaagcat
     1921 tttccccgga tctgtttacc cgctagattt gcaaaaaaaa gccctgaatg cgcagaaaga
     1981 gcctgcttgt cattatcgcc ctgctgatca tcattgccgt tattgcctgg gcgccccttc
     2041 attttgaaat aaagaaagaa atccggattg aaacgcccat agaatacgtc ggccgccaat
     2101 tcaccgatct ccgccattgg aaaaactgga acgttgccgt tcgcaggctg gatagttcgg
     2161 gaataaagta ctccgcttcc tcctccggca tcaacgcctt tctgcagtcg ggagacctcc
     2221 tgtataccat tatccaaacc aatccggcct ttgtgctggt cagatccgga cagcccggaa
     2281 ccagggaata tcattcgctc tacgccgttt ccgactcctt cgggaagtcg accgaagtca
     2341 cctggacccg gtctctcccc ccggcgagat ggttgaaaga aaagatcatt ccggacggcg
     2401 aaatggaaga gggtttgaaa aatttaaaga atacgctgga agaccccttg catatatatg
     2461 gatttcccat ccgcatcggg cccgtaacag ataccctggt catcacctgc accgcaacct
     2521 gtgccaaaaa ggataagatc gccgaactca accggttgta ccagggcatt tacgggtatg
     2581 caaaaagaag aaagatgccc gtcgcggatt cgcggatggc caatttcact ttccggggaa
     2641 aggatagttt gcagatcgat gccggaattc ccacatcggt aaacggggac ccggagaaca
     2701 atatctcggt tcttcgcatg cccacccgcg ggagaatgct gatcggcagc tatgaaggtc
     2761 cttacagcgg tttgggtaaa ctttattcgg caatggggaa atatacccgc gacaaacggt
     2821 tgatgaaagt ggctgctgtt tatgaaaaat atttgactga ccctaaatcg gcaacggaca
     2881 gcctccatat gaagatcgaa ctctgttttc cggtctttta ataagaaaga aaaatcaatt
     2941 tttcattctt tctggcaagc accaggaatc gttgatccct cacctgcagg gtttgcatgt
     3001 cccgaacttc tccggagatc aacagattgt cggcaaaggg tatcctgcag gtaaaatttc
     3061 ctttgccgtc tccgattccc agcgtggggg gcatggcatc atacctgcct tcaaagggcc
     3121 tcgttccgaa aaaattgccg gcagccagaa ggtctttttt cccgtcgtga tcaaaatcac
     3181 cggtgtaaaa ggagaagatg ggagcccatt gcatcggtac cggcaagggt ttaacgataa
     3241 atcccccctt gcggtcattc atcaaaacga cggaagccag cgtcgacgcc tgcagcaggt
     3301 ccgcctttcg taatttttct ccgaaaattt cctccatcgt tttccccgcc attttggaat
     3361 agctcaggta ttcttttttc agatagggca attgtttctc cagctcttcc ttacccagga
     3421 aaggataata tttgccgtct ttctgaatgg ataataattg ttccgggatc ccattggcat
     3481 caaaatcgcc cacatacatt ttaaccggat attcggcaga agctttcagc ttcgaattca
     3541 acccgtaatt acccagcagg aggtcgtcat agccatcccc gtccaggtct gtgcttttga
     3601 tgcaggtcca ccagcccgtc attccatcca aagaggtatg ctctttttcc agccggccgt
     3661 tccggttaat gaaaatcgcc ggagccatcc agtctcccac gacaaccaga tcgggcctgc
     3721 catcccggtt gatgtccgtc cagcaggcat ccgtaatcat gcctgccatc ttcaacccgg
     3781 gagccaccgc atcggttacg atagcaaaat ttcccctgcc gtcattgtgt agtaaataag
     3841 actgcggaac agttccataa tttcccgggt tcgccctgcc accgataaaa aggtctttat
     3901 atccatcccc gtcataatct gccgccctga ccacggaccg gttgccggac agaagcggaa
     3961 ggcctgccga ccttgtaaaa ttcccattgc cgtcattgag atagagccgg tcctgctcca
     4021 aatctccctt agatgccgaa atattcccgc cgttgacgac atacaggtcg ggatccccgt
     4081 cctggtctgc gtcaaagaaa agggcatcct ccttttcgca ggccgcatcc ttcccaaaca
     4141 aagaatcgtc gcctgatttg aaagaaccgt cgcgctgctg aatatataat tttgccggct
     4201 cgccctttcc gccgcacaca aaaaaatcct ccaacccgtc cgcattcacg tcggcgaccg
     4261 cgagctttgg ggccagcggg gacaacaggt gaggaataag cggttgccgg ttaaaatcat
     4321 tgatccccgc cggattttcg aaatccttaa aatccgggct caggggaacg gggacattgg
     4381 agaaaaaaga agtgtccggc tctttcaata agcggttgat aaattgccgt tcatccgtaa
     4441 tcgtatctga tttccggcgg tcatatggta tgaccagctt ctggtcggcc ggaacctttt
     4501 taagccgctg aaaggtatta tccggccaga tcacgaggag gctgtcggct acggccgaat
     4561 ggtttaatcc aaaatgcaac aacggttcca cactgctcat gaaacctttc accgtttgca
     4621 attcccggta caataattta cccgcgtaaa aaacaaaaac cttggcgccg atggcaaacg
     4681 tattggggga agcgcctttt aattgcacgg taaggtaatg tgatcccggg ctgcgggtcc
     4741 ggatagtatt ccggtaaatg cccgcctcac tgttcatatt gttggtgacc aggtcgagcg
     4801 cgccgtcgtt atccaggtcg gcataggctg caccctgcga aagatccgcc tttccgaatc
     4861 cccagtcagc ggatctgtcg gtgaaccgca gaacggaatc tccttcaaaa atataattat
     4921 gcacggctcc cggaggctgc tggctaagga tttcccggtc atgactgcgg gtttcattgt
     4981 accggatatt catctggttt ccggagagga acttttcata atcgaggtca ttgagcctgt
     5041 tcctgatccc gttgctgata aaaatatcgt cgatgccgtc caggttgaaa tccgcgatca
     5101 ggggactcca gctccaatcc gtggcagcaa tgccgctata caggccgata tcagaaaacc
     5161 atttgcccct tcccacattc agctgcaggc aatttctgga atactggtaa tggtaacccg
     5221 cttttatttt cagcctgtaa ttatcgtagg tttcatccgt agcggaagat ttcaatacct
     5281 tctcgtcctc cggcagcatg tccagcgtca gcaggtcagg ccagccatcg ttgttgacgt
     5341 ctgcgatatc gcttcccata gagtacttgg attcatgccc gaatgcctgg gcattcattt
     5401 ccctgaaccg gccgttgccg aggttcaggt aatagtaatc attctcatga aagtcgttgc
     5461 tgacgtaaat atcgtcgtac ccgtcgtggt taaaatcggc aacgcccacg ccaagtccgt
     5521 aacccagcga actgctgatg atccccgaac cagcggtcac gtcccggaaa tggttccctt
     5581 cgtgatgaaa caatttcccg ccgctgactg cactgtattt gctgcgcacc gagctgtccc
     5641 cgtaatttgc cgtctgatga atgga
//
LOCUS       DASYCU010000364         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3992865, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000364 DASYCU010000000
VERSION     DASYCU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3992865"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..846)
                     /locus_tag="VGM24_07395"
     CDS             complement(100..846)
                     /locus_tag="VGM24_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4155234.1"
                     /translation="MQSIKNIFGRVWAVWGALAFVATMLVFLIPFFGFCYFQKDPKKT
                     RRFILFSRTWMDVYLFLIGCPLKIKGRENFKKGETYIVVCNHNALIDVPVSSPGIPGG
                     NKTIAKSEMAKIPVFGLIYQTGSILVNRKDEKSRRDSYMKMHEVLNMGLHMCIYPEGT
                     RNTGNEPLKPFHDGAFRLSLGSGKSIVPMIIFNSRRANPPTRGFFLLPVKLYMDFLPE
                     IKPLPGESVDLLKARVHEIMKRYILDHAPN"
     gene            complement(867..1538)
                     /gene="ung"
                     /locus_tag="VGM24_07400"
     CDS             complement(867..1538)
                     /gene="ung"
                     /locus_tag="VGM24_07400"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY4155235.1"
                     /translation="MNVQIEPLWKKVLDTEFSKPYFLQIVHFLKTEKNTGKTIYPPGQ
                     LIFNAFNTTPFDRLKVVLLGQDPYHGLGQAHGLSFSVPDGIRPPPSLQNIFKELQSDT
                     GSGIPQTGNLTPWARQGVLLLNAYLTVLAKAPMSHSQNGWGDFTDAVIRKISDLKKNI
                     VFLLWGKFAQEKQGLIDETKHLVLKAAHPSPYSADKGFFGCRHFSKTNEYLVKNGIDP
                     IDWKL"
     gene            1645..>2519
                     /locus_tag="VGM24_07405"
     CDS             1645..>2519
                     /locus_tag="VGM24_07405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4155236.1"
                     /translation="MKYRNLGKTGQRLSAIGLGCMGMSFAYGPRDDRESIATLNRSLE
                     LGVNFWDTADMYGMGHNEELISTVLVPNRDKIFLATKFGFRQRVGTSGLAGTPGTYFD
                     GSPAHVKEAVEDSLRRLKIETIDLYYAHRVDQNVPVEDTVGAMAELVKQGKVKWLGLS
                     EASAVSIRRAHAVHPITALQSEYSLMTRDVEGEILATVRELGITFIPYSPLARGLITA
                     TVSEKDRLPADDFRRTVPRFDAEHWENNQQLVNEFAGIAMQEDCTPAQLALAWVLAQG
                     EDIIPIPGTKKRKYL"
BASE COUNT          641 a          604 c          654 g          620 t
ORIGIN      
        1 tcaatgttac gccggttggc ttataccgtt cattcagcat aacccttaat ccgaaatccg
       61 gaatactccg ggacctgcgc atcaaccgga accggacctt taattaggag catgatccag
      121 gatatatctc ttcattattt cgtgcacacg ggctttcagc aaatccacgg attcgccggg
      181 taagggcttt atttccggaa gaaaatccat atagagttta accggaagca gaaagaatcc
      241 cctggtcggc gggtttgccc ggcggctgtt gaaaatgatc atgggcacga tcgattttcc
      301 gctgcccagg gagaggcgga aagcgccgtc atgaaaaggc ttgagcggtt cattgcccgt
      361 attgcgcgtt ccttccggat aaatgcacat gtgcaggccc atgttcagca cttcatgcat
      421 tttcatatag ctgtcgcgcc tgcttttttc atctttccgg ttcaccagga tgctcccggt
      481 ctgatagatc aatccaaaaa ccgggatttt cgccatttcg gatttggcaa tggttttatt
      541 tcccccgggt ataccaggcg aggaaaccgg gacgtcaatc agtgcattgt gattgcatac
      601 cacgatatag gtctccccct ttttgaaatt ttcacggcct tttattttaa gggggcagcc
      661 gatcaggaac aggtagacat ccatccaggt cctcgaaaac aggatgaagc gcctggtttt
      721 cttagggtct ttttgaaaat aacaaaaacc aaaaaaaggg atcagaaaaa caagcatggt
      781 cgccacaaaa gccagtgctc cccaaactgc ccagacgcgc ccgaagatgt ttttaatgga
      841 ttgcattatg tttaaatttc cgggagttaa agtttccagt caatggggtc aatcccgttt
      901 ttcaccaggt attcattggt cttggagaaa tgacggcatc cgaaaaaacc tttgtcggca
      961 gaatatggag aaggatgggc cgctttcagc accaggtgtt ttgtttcatc gatcaacccc
     1021 tgtttttcct gggcgaattt tccccagagc aggaacacga tatttttttt caggtcggaa
     1081 attttccgga taacggcatc ggtaaaatcg ccccagccat tctgggaatg gctcatgggt
     1141 gctttggcaa gaaccgtcag gtaggcattg agcagcagga cgccctgcct ggcccagggg
     1201 gtcagattcc cggtttgcgg aatgccagat ccggtatccg attgcaattc tttaaaaata
     1261 ttctggaggg agggcggggg acgtatcccg tccggaacag aaaaacttaa tccgtgcgcc
     1321 tgacccaacc cgtggtaagg gtcctggccc aggaggacca ctttcaatct atcgaaaggg
     1381 gttgtgttaa acgcattgaa aatgagctgg ccgggcgggt aaatggtctt gcctgtattt
     1441 ttctctgttt taagaaaatg gacgatctgc agaaaataag gtttggaaaa ttcggtatcc
     1501 agcacttttt tccacagggg ttcaatttga acattcatac agcgatcctg ataagtaaca
     1561 aatgtataat catcttttcc acaaacccat attcattggc cctaattttg tccagagaat
     1621 tattcaccgc taaaagaata ggatatgaaa tacagaaatc tgggaaaaac aggacaaagg
     1681 ctgtctgcca tcggtctggg ctgcatgggc atgagcttcg cctacgggcc gcgggatgac
     1741 cgcgaatcca tcgccaccct gaaccgttcg ctggaactgg gagtaaactt ctgggacacg
     1801 gcggacatgt acggtatggg tcataacgag gagctgattt cgaccgtgct ggtgccgaac
     1861 cgcgataaaa tattcctagc caccaagttt ggttttcggc agcgggtggg aacttccggc
     1921 cttgccggaa cgccgggcac ttattttgac ggctccccgg cgcatgtaaa agaggcggtt
     1981 gaggacagcc tgaggcggtt aaaaatcgaa accattgatt tgtattatgc tcaccgggtc
     2041 gatcagaacg ttccggttga agataccgtg ggcgctatgg ccgagctggt aaaacagggc
     2101 aaggtaaaat ggctgggttt atccgaagcc tccgctgttt ccatccggcg cgcgcatgcc
     2161 gttcacccca tcacggcatt acaaagtgaa tattcattga tgacccggga tgtggaagga
     2221 gaaattcttg ctacggtgcg ggagctggga attaccttca ttccctattc gcccctggcc
     2281 cggggcctga tcacggcaac agtgagtgag aaagaccgtt tgcctgccga tgatttcaga
     2341 aggacggttc cccgcttcga tgcggagcac tgggaaaaca accagcagct ggtaaatgaa
     2401 tttgccggga ttgcaatgca ggaagactgt accccggccc aactggccct ggcctgggtg
     2461 ctggcacagg gagaagatat tatccccatt ccgggtacca aaaaaagaaa atacctcga
//
LOCUS       DASYCU010000365         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3414707, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000365 DASYCU010000000
VERSION     DASYCU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3414707"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGM24_07410"
     CDS             <1..252
                     /locus_tag="VGM24_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4155237.1"
                     /translation="LPDKTGGIEIGYGTMPSHRGKGIMTEAVGGMIQWAKSRPDIESI
                     LAETDKDNKASICVLEKNQFILFDVRENMKWWRINVSGL"
     gene            complement(241..1587)
                     /gene="purB"
                     /locus_tag="VGM24_07415"
     CDS             complement(241..1587)
                     /gene="purB"
                     /locus_tag="VGM24_07415"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY4155238.1"
                     /translation="MELNSLTAISPLDGRYRSQLQQLSEYFSEYALIKYRIRVEVDYF
                     LFLAEKKFFAISAATRKQLESVAENFSVEDAREIKKIEKTTNHDVKAVEYFLKSRMEL
                     SKDADTREWIHFGLTSQDINNTAIPLLWKHALEIEYLPFVLNLKKQLRLLAGPWIRMP
                     MLARTHGQAASPTLLGKEIMVFVERLENQLELLIQIPFTAKFGGATGNFNAHHVAFPK
                     KDWVKLANEFVENKLGLKRQQYTTQIEHYDMLAAHFDSIKRINTILVDFCRDIWTYIS
                     MDYFKQKTREGEIGSSAMPHKVNPIDFENAEGNLGLANTIFEYLSAKLPVSRLQRDLT
                     DSTTLRSIGVPFAHTLLALKSIERGITRLVLNEKKLKQDLEDNWAVVAEAIQTILRRE
                     DYPKPYEALKDLTRGKNGITRESIHHFIDSLKIAKVVKEELKKITPHNYTGVSLKA"
     gene            1660..2400
                     /locus_tag="VGM24_07420"
     CDS             1660..2400
                     /locus_tag="VGM24_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEY4155239.1"
                     /translation="MDRKIRVGAVSYLNTKPLLYGIERSPLLEKMELITDYPASIAAL
                     LLKDEIDIGLVPVAIIPEMKEYHINTRYVIGCNGPVASVCLFSAVPVEKLETVLLDYQ
                     SRTSVALVKILLREYWKINPVFKEAGPGFQDEIKDSVGAVMIGDRALKQRSVSAYTYD
                     LGEAWKKMTGLPFVFAAWISNKKLDPEWVSAFDQANGVGVHHIDQVLEGLDNSLFNLK
                     KYYTQYLSYELDPEKEKGMQAFLEMMRR"
     gene            complement(2426..>3004)
                     /locus_tag="VGM24_07425"
     CDS             complement(2426..>3004)
                     /locus_tag="VGM24_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEY4155240.1"
                     /translation="LRITDPVCGMSSSFPVNVIVNEKPVVKALKSNDIDCQTGTSQLN
                     ATGARSYEWLPAAATVDSHIADPMVAIESTTTFTVTGMDENGCTSTDTVTVLVTSKGK
                     ALMVLPNAFTPNHDGLNDCFGVIKWGNVTIEEFSIFNRWGQRVFDTRNAGDCWDGTFH
                     GEMQDTGQYVYVIKAASFCGNITRTGTVLLIR"
BASE COUNT          762 a          715 c          735 g          792 t
ORIGIN      
        1 ctaccggaca aaacgggggg cattgagatc gggtacggca caatgccgtc ccacagggga
       61 aaaggcatta tgacggaagc ggtcggcggc atgatccaat gggcaaaatc gagaccggat
      121 attgaatcta ttctggcaga gacggataaa gataacaagg cctccatctg cgtactggaa
      181 aagaatcaat tcatcctgtt tgatgtccgg gagaatatga aatggtggag aataaacgta
      241 tcaggccttt aagctgacgc cggtataatt atgcggtgta atttttttca gttcttcttt
      301 caccactttt gcgatcttca gggagtctat aaagtggtgt atggattccc gggtaattcc
      361 gttcttcccc cgggtgaggt cttttagggc ttcatagggt ttcggataat cttccctccg
      421 gagaatggtt tggatggctt ctgccaccac cgcccagtta tcctccagat cctgtttcag
      481 ctttttttca ttcagtacga gccgggtaat tccccgctca atggatttta gagccagcag
      541 ggtatgtgca aacggcacac cgatgctccg tagggtagtt gagtcggtca ggtcccgctg
      601 caggcgggaa acaggcagtt tggcggacag gtattcaaaa atcgtattgg ccaggccgag
      661 gttgccttcc gcattttcga aatcgatcgg atttacctta tgcggcatgg ccgatgagcc
      721 gatctctcct tccctggttt tctgtttaaa ataatccatg gaaatatagg tccagatatc
      781 gcggcaaaaa tccaccagga tcgtattgat ccgtttaatg gagtcgaaat gagccgccag
      841 catatcatag tgttcgatct gggtcgtgta ttgctgtctt tttaagccca gcttattctc
      901 tacaaactca ttcgccagct tcacccagtc ttttttcgga aaagccacat gatgggcgtt
      961 gaaattgccg gtggctcccc cgaatttggc agtgaatgga atctggatca acagttccag
     1021 ctggttctcc agtctttcca caaataccat gatctccttt ccgagcaggg tgggagaggc
     1081 agcctgtccg tgcgttctgg ccagcatggg catacggatc cacggaccgg ccagcaaccg
     1141 gagttgtttc ttcaaattaa ggacaaaggg taaatattct atttccagcg catgtttcca
     1201 gagtaacgga atggccgtat tatttatatc ctgggaggtg agaccgaaat ggatccattc
     1261 ccgggtatcg gcatccttgc tgagttccat tctggatttc aggaaatatt caacggcttt
     1321 tacatcgtgg ttcgtggtct tctctatctt tttgatctcc cgcgcgtcct ccacactgaa
     1381 gttttcagct acagactcca gctgcttacg ggtggcagca gagatcgcaa aaaatttttt
     1441 ttcggccaga aataaaaaat aatccacttc cacccgaatc cggtatttga tcagcgcata
     1501 ctccgagaag tattcgctca gctgctggag ctggctgcgg taccttccgt cgagcggaga
     1561 aatggccgta agggaattca gttccatata ttcgtttgcc ggtgaaagaa atattcaggt
     1621 tgcttagcct acttttgcgc caaatttaaa ctaatcgcgt tggacagaaa aataagggta
     1681 ggggccgtga gttatctgaa caccaaaccc ctgttatatg gcattgaacg gtcaccttta
     1741 ctggagaaaa tggagctcat caccgattac cccgccagca tcgcggctct tttgctcaaa
     1801 gatgaaatcg atatcggcct tgtacccgtt gccattatcc cggaaatgaa ggaatatcac
     1861 atcaataccc ggtatgtgat cggctgtaac ggtcccgtgg catccgtttg cctgtttagc
     1921 gcggttccgg tggaaaaact ggaaacggtc ctgctggact accagagccg gacttcggtg
     1981 gccctggtaa aaatcctgtt aagggaatac tggaaaataa accccgtgtt taaggaagcc
     2041 ggtccgggct tccaggatga aataaaagat tctgtcgggg cggtgatgat aggcgaccgg
     2101 gcattgaaac aaagatccgt ttccgcctat acctatgatc tgggagaagc ctggaaaaaa
     2161 atgaccgggc ttccgtttgt attcgctgcc tggatcagta ataaaaaact ggatccggaa
     2221 tgggtaagcg cctttgacca ggccaatgga gtcggcgttc accatattga ccaggtgctg
     2281 gaagggttag acaacagcct cttcaacctg aaaaaatact acacacaata ccttagctat
     2341 gagctggacc cggaaaagga aaaaggaatg caggcctttc tggaaatgat gaggagataa
     2401 accggaagat tgttcatgac cagaactatc ggatcaggag taccgtgccg gtccgggtaa
     2461 tatttccgca aaaggaggct gcttttataa cataaacata ttggccggta tcctgcattt
     2521 ccccatgaaa agttccatcc cagcaatccc ctgcattccg ggtgtcaaaa actctttgtc
     2581 cccatcgatt gaaaatggag aactcttcta ttgttacatt cccccatttt atcactccaa
     2641 aacaatcgtt aagtccgtcg tggttcggag taaatgcatt gggtaatacc atcagcgctt
     2701 tccccttact cgtaaccaaa acagtcactg tatccgttga ggtacagcca ttctcatcca
     2761 taccggtgac ggtaaatgtc gtagtgcttt ctatggctac catcggatct gcaatgtggg
     2821 aatccactgt tgcggcagcg ggtaaccatt cataagacct ggcacccgtt gcattcaact
     2881 gactggtccc ggtctggcaa tcgatatcat tgctttttag agccttaacg actggctttt
     2941 cattgactat cacgttaacc gggaaactgg aactcatccc gcataccgga tccgtgatgc
     3001 gtag
//
LOCUS       DASYCU010000366         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3993689, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000366 DASYCU010000000
VERSION     DASYCU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3993689"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /locus_tag="VGM24_07430"
     CDS             <1..1670
                     /locus_tag="VGM24_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY4155241.1"
                     /translation="NLHTYLDIELQELAEKLLSNKVGAIVAIEPKTGGILAMASSPDY
                     DPNSLTGPDKQKNYGKLVLDVRAPLLNRAIKGLYQPGSTYKPLAALIGLDEGVITPAS
                     GIACTGFYYGCNKVYRCTEKAPGHAANLRLAIANSCNSFFYNAFKLTLDNPELHGPRN
                     GLMKWKEYVNKFGLGERLGVDLPSEDAGNIPDTLTYDKEYHGSWNSCTMVTMGIGQDK
                     MQVTPLQIANAMCIVANKGSFYIPHFVERVDGESPDDSILNKYSQKHAVLNHISDADY
                     ETVISGMQDVTEIGTARMARIPGINMCAKTGTAETYLSLEGRRTKLNNNSIFVCFAPR
                     ENPQIVVAVVVQNAGFGARWAAPMGSFLVEKYLRDTLTAERVKQATVIANTNLVPGYF
                     VRLQFLTDSLRGEAWAKQTGDSSRLKRYQDPSLRREMLDTLHLPTVRVTIPFGPRPAA
                     AASHGDTAKSRKSDSVLLKKAEPHRTHKADSLPLYRTDSGGLHKADSTKIRRPVPGRS
                     NNPDSGSLKGAETRLMNKNSSRWADYYALKPEEQKSVQPDDLKNSRS"
     gene            1667..>2706
                     /gene="rodA"
                     /locus_tag="VGM24_07435"
     CDS             1667..>2706
                     /gene="rodA"
                     /locus_tag="VGM24_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY4155242.1"
                     /translation="MNQRNASLGKGVDRSLILLWFVLSVIGVISIFAATYRDGENILQ
                     GFLSLKTDYSRQALFFLLSGIAGIFILLTDSKFFTATANLWYAFGLLLMLLVFPFHSN
                     IKGTESIIRLGSAVQIQPADICKIFVNLSLAKYLSRVETDFAKPRSQIIAAFIALIPA
                     GLSIMQKETGLALVYFSFFLVMFREGLPPYVLIVGFSFAVLVVATLLLDPNLLALLLT
                     GIAALAIFILRKQVKRHRSILFFIASIWFVCVGIQRFVVPFAFDHIFQPYQVKRIYDA
                     IGKDYIPKNAEAALEIEQKSGAKKKDNSNYNVKQSKIAISNGGLLGKGLLSGTQTRYD
                     FVPEQRTDFIFCT"
BASE COUNT          692 a          701 c          671 g          642 t
ORIGIN      
        1 ggaacctgca cacctatctc gatatcgaat tgcaggaact cgctgaaaaa ttgctgtcca
       61 ataaggtcgg agcgatcgtg gccattgaac caaaaacagg aggcatcctg gccatggctt
      121 ccagtccgga ttacgatcca aactcgctta cgggtcccga taagcaaaag aattatggaa
      181 aactggtgct ggacgtccgg gcaccgctgc tgaaccgggc catcaagggg ctttaccagc
      241 ccggatccac ctataaaccg ctggccgccc tgatcggcct cgatgagggc gtcatcacgc
      301 cggccagcgg cattgcctgc accggattct attacggctg caacaaagtg tatagatgta
      361 cggagaaagc gccgggacat gcggccaatc tccgcctggc cattgccaac tcctgcaatt
      421 ccttctttta taatgcattc aagctaaccc tcgataatcc ggagttgcac ggaccaagaa
      481 atggattgat gaaatggaaa gagtacgtta acaaattcgg actgggtgaa cgccttggag
      541 tggatcttcc gagtgaagac gcaggaaata ttcccgacac cctcacgtac gacaaggaat
      601 atcacggttc ctggaattcc tgtacgatgg tgaccatggg tatcggccag gataaaatgc
      661 aggtaacgcc cttgcagatt gccaacgcca tgtgcattgt tgccaataag ggatctttct
      721 atatcccgca tttcgtggaa agggtggacg gcgaatctcc cgatgattcc attctgaata
      781 aatacagcca aaagcacgcg gtgctcaacc atatttccga cgctgactac gaaacggtca
      841 tcagcggtat gcaggatgta accgaaatcg gcacggcgcg catggcgcgg ataccgggca
      901 tcaatatgtg tgcaaaaaca ggcacggcgg aaacctactt atctctcgaa ggtcgcagga
      961 ccaaattaaa taacaattcc attttcgttt gttttgcacc ccgggaaaat ccccagatcg
     1021 tggtcgcggt ggtggtacag aatgcaggtt tcggggccag atgggccgca cccatgggca
     1081 gcttcctggt tgaaaaatat ttgcgcgata cacttaccgc cgaacgggtg aaacaggcta
     1141 ccgtgattgc caataccaat ctggttcccg gttattttgt gcgccttcag ttcctgaccg
     1201 attccctccg gggcgaggcc tgggccaaac aaaccggcga cagctcccgg ctgaaacgct
     1261 accaggatcc gtcactccgc agagaaatgc tggataccct gcatctgcca accgttcggg
     1321 taaccattcc cttcgggcca aggccggcgg cagcggcttc ccacggagat acggcgaaat
     1381 ccaggaaatc agattcggtc ttattgaaaa aagcggaacc gcacagaaca cataaggctg
     1441 attcattgcc attatacaga acggattccg gcggattgca caaagcggat tccactaaaa
     1501 tcaggaggcc cgtacccggc cggtcaaata atcccgattc cggcagctta aagggtgcag
     1561 aaaccagatt gatgaataaa aattcttccc gctgggcaga ttactatgcc ctgaagcccg
     1621 aagaacaaaa gtccgttcag cccgatgatc taaaaaacag ccgctcatga atcaacgtaa
     1681 cgcttctttg ggaaaagggg tggaccgctc gctgatcctg ctgtggttcg tcctgagcgt
     1741 catcggagtc atcagtattt ttgccgccac ctaccgcgac ggagaaaata ttttgcaggg
     1801 atttctttcc ctgaaaacag actacagccg ccaggccctg ttttttttat tgagtggaat
     1861 agccggcatt ttcattttgc tgacagacag taaattcttt accgcaacgg caaatctctg
     1921 gtatgccttc ggactcctgc tgatgctgct ggtgttcccc tttcattcaa acatcaaagg
     1981 gacggagtcc attatccggt tgggaagtgc cgtccagata cagcctgcgg atatttgcaa
     2041 aatatttgtg aacctttccc tggcaaaata tttatcgcgg gtggaaacgg attttgccaa
     2101 accgcgttcc cagatcatcg ccgcctttat tgcgttgatt cccgcaggcc tcagcatcat
     2161 gcaaaaagaa accgggctgg cgctggttta tttttcattt ttcctggtca tgttccggga
     2221 aggacttccg ccctatgtac tgattgtcgg gttttccttt gccgtgctgg tcgtagctac
     2281 gcttttgctg gacccgaacc ttctggccct ccttctgacc gggattgccg cactggccat
     2341 ctttatttta agaaagcagg tgaaacgcca ccggagtatt ctattcttca ttgccagcat
     2401 atggtttgtt tgtgtaggca ttcagcgttt tgtggtaccc tttgcttttg atcatatctt
     2461 ccagccatac caggtaaaac gcatttatga tgccatcgga aaggattata ttccaaaaaa
     2521 tgcggaagcc gccctggaga tcgagcaaaa gtccggagct aaaaagaaag ataacagtaa
     2581 ttataatgta aagcagagta agattgccat cagtaacggc ggcctgttgg gcaaaggcct
     2641 actgagcggc acccaaaccc gctacgattt cgttccggaa cagcgaacgg attttatatt
     2701 ctgtac
//
LOCUS       DASYCU010000367         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4138457, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000367 DASYCU010000000
VERSION     DASYCU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4138457"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VGM24_07440"
     CDS             complement(<1..1374)
                     /locus_tag="VGM24_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEY4155243.1"
                     /translation="MTKFFIFGIMLQESLLLILSLLFAVSMLTILSKKLHISYPIFLV
                     ISGLLIGFIPGIPAVSLDPDFVFLIFLPPLLYAAAWNTSWKDFWEQRRNISLLAFGLV
                     IFTSAAIAFLSHAMIPGFSLALGFLLGGIISPPDAVAASSVLEELKIPKRIVTILEGE
                     SLINDASSLIVFRFALLAVITGEFTFWKATGDFFLVGGLGIVTGLGIAYIIYNIHRLL
                     PTSPSVDTALTLISPYLMYIAAEHFHYSGVMAVVSGGLFLSFRSHIIFTYNSRIQIYS
                     FWETLNFLLNGMVFILIGLQLPQIVGGLNEYSLSTVIFYAVVISLVTIIIRIIWVFPG
                     AYIPRLLSRKIRIGENRPPWKSVFLVAWSGMRGVVSLAAALAVPLTLSGGKAFPQRDL
                     ILFITFIVILFTLVLQGLSLPWLIRVLKIGDAGEDQTAEQEAAIRLRLAWAALNHMQN
                     HYSGELNE"
     gene            complement(1441..2118)
                     /locus_tag="VGM24_07445"
     CDS             complement(1441..2118)
                     /locus_tag="VGM24_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HEY4155244.1"
                     /translation="MRSGFLISFALFIPFLLLVTAQSVFAQQAPGSPAPSPKSVTARQ
                     PPESRFGKNDTIYSMIIVYDGDTIPAQTLSLVSVARAMTRAQRKQWEAMTRLRNAVYV
                     TYPYARKAGIILNEMNGVLAKMKSEEQKKGYIKSREKDLKKEFTDPMENLSVYQGKVL
                     MKLINRQTGNNCYDIIKEYRGGFTARFWQTVAFFFGSSLKQPYDARGDDQEIELIVQE
                     VERMYRG"
     gene            complement(2150..>2895)
                     /locus_tag="VGM24_07450"
     CDS             complement(2150..>2895)
                     /locus_tag="VGM24_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HEY4155245.1"
                     /translation="NITLLKNVKTDMGKYYATYLSNDSVNPAGNIIYFQINFQNKRTG
                     ESFDLWPNLIKNTKGVENYSNNPDKRHYLDRDLFTYISYANILDKQKDTSGFVSHPVA
                     LHDSIFYSKGYMILDTVLVNPNTDKYHFTSSDTALLARIRVVSQDGSQYTISPLLYVK
                     NNMVNRVTDTLFAQNLAVELGSVMNNRKIELRVKESSDMVPFVSLKVYAFPQINLVWI
                     GIVVMVTGFWMSIFYRRKQRLRPEPPLVV"
BASE COUNT          782 a          705 c          743 g          665 t
ORIGIN      
        1 ctcatttaat tccccgctat aatggttctg catatgattc agggcggccc aggccaggcg
       61 aaggcggatg gccgcttcct gttctgcggt ctggtcttct ccggcatccc cgatcttcag
      121 tacgcggatc agccagggta agctcaggcc ctgcaaaacc agcgtgaaca aaataacgat
      181 gaacgtgatg aagaggatca ggtcccgttg cgggaatgcc ttcccgccgg ataaagtaag
      241 cggaacggcg agcgcggctg ccagcgatac caccccccgc atgccgctcc aggcgaccag
      301 gaaaacactt ttccagggag ggcggttttc cccgatccgt attttccggc tcagcaggcg
      361 gggtatataa gcgcccggga aaacccagat gatccggatg ataatcgtga ccaggctgat
      421 aacgaccgca tagaaaataa ccgtggaaag ggaatattcg ttcaggccgc cgacaatctg
      481 cggcaattgc agcccgatca aaataaagac catcccgttt aataaaaagt tcagggtttc
      541 ccaaaaagaa tagatctgaa tgcgggagtt ataggtgaaa atgatatgcg accggaagct
      601 tagaaacaga cccccgctca ccaccgccat tacaccggaa taatgaaaat gctcggccgc
      661 gatatacatc aggtagggag aaataagcgt taacgccgta tccacgctcg gcgaggttgg
      721 caaaagccgg tggatattat aaatgatata ggcaatccct aatccggtca cgatacccag
      781 tcccccgacc aggaagaaat cgccggtcgc cttccagaaa gtgaactcac ccgtaataac
      841 cgccagcagg gcaaaccgga agacaatcag ggaggaggca tcgttgatta aactttctcc
      901 ttcgaggatg gtaacgatcc gttttggaat ttttaattcc tccaataccg aagaagccgc
      961 caccgcatcc ggcggagaaa tgattccgcc cagtaaaaag cccaatgcca gagaaaatcc
     1021 gggaatcatg gcatgcgata aaaaggcaat ggcggcagaa gtgaagatga ccagcccgaa
     1081 cgccagcagg ctgatgttcc tccgctgttc ccaaaaatcc ttccaggagg tattccaggc
     1141 cgcagcgtag agcaatggcg gaagaaagat caaaaaaaca aaatcaggat cgaggcttac
     1201 ggcgggtatg ccgggaataa agccgatgag caggccggaa atgacgagga atatcggata
     1261 ggaaatatgt aatttcttgc tgagtatggt cagcatggac accgcaaaca ataaagaaag
     1321 gatcaacagt aagctttcct gcagcatgat tccgaaaata aagaattttg tcaaatgttc
     1381 cttttccgga gaggaatcga taccggggaa taaaggcaga aagaccgcaa taagtaatag
     1441 ctagccccgg tacatccttt ccacctcctg cacgatcagc tcaatttcct gatcgtcccc
     1501 acgggcatcg tagggttgtt tgaggctgct tccgaagaag aaagcaacgg tctgccagaa
     1561 acgggcggtg aatccgcccc ggtattcttt gatgatgtcg taacaattat ttccggtttg
     1621 acgattgatg agtttcatga ggacctttcc ctgataaacg gaaaggttct ccatcggatc
     1681 ggtaaattct tttttcagat ctttctccct ggatttgata tatcctttct tctgctcctc
     1741 cgatttcatt tttgccagca ccccgttcat ctcattgagt atgatcccgg cttttctggc
     1801 atagggatag gtgacatata cggcattgcg taaacgggtc atggcttccc actgcttacg
     1861 ctgggcgcgg gtcatggcgc gggcaacact gaccagcgaa agggtttgtg ccggaatggt
     1921 atctccgtcg tacacgatga tcatgctgta gatggtatcg ttctttccaa agcggctctc
     1981 gggcggctgc cgcgcggtta cggatttggg agaaggtgcc ggggatccgg gtgcctgctg
     2041 ggcaaaaacg gattgcgccg tgaccaatag gaggaacgga ataaacaggg cgaaagatat
     2101 caaaaacccg gatcgcataa tgaccctaaa gttaaagatt attgcgggtt catacaacca
     2161 gcgggggctc cggccgtaaa cgctgtttcc gccggtaaaa tatgctcatc cagaagccgg
     2221 tgaccatcac cacgatcccg atccagacca ggttgatctg tgggaaggca tatactttta
     2281 acgatacgaa cggaaccatg tcgctggatt cctttaccct taattcaatc ttccggttgt
     2341 tcatcacgga tcccagctcc acggccaggt tttgggcaaa aagggtgtcg gtcacccggt
     2401 tgaccatatt gtttttcaca tatagcaagg gggaaatggt atactgcgaa ccatcctggc
     2461 tgaccacccg gatccgggcc agcagggccg tatccgatga agtaaaatgg tatttatccg
     2521 tgttcggatt caccagcacg gtatcgagga tcatatatcc ctttgaataa aaaatgctgt
     2581 cgtgcagggc aacgggatga gaaacaaaac cactggtatc tttctgttta tccaggatat
     2641 tcgcataact gatgtaagta aacagatcgc ggtccaggta gtgtctttta tcggggttat
     2701 tcgagtagtt ctccacgcct ttggtgttct tgatcagatt gggccaaaga tcaaacgact
     2761 cgccggtgcg cttgttctga aaattgatct ggaaataaat aatatttccg gcagggttca
     2821 cggaatcatt gctgagatag gtggcgtaat atttccccat gtccgtcttt acatttttca
     2881 gcagcgtaat attct
//
LOCUS       DASYCU010000368         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_516445, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000368 DASYCU010000000
VERSION     DASYCU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516445"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..526)
                     /locus_tag="VGM24_07455"
     CDS             complement(287..526)
                     /locus_tag="VGM24_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155246.1"
                     /translation="MLTIYPNSATSILYLNTGSSLITVNSLKVYSINSVLVYAVNSIL
                     PSSYAINIGDFPRGAYTAILELSDGSRQVREFVKQ"
     gene            786..1400
                     /locus_tag="VGM24_07460"
     CDS             786..1400
                     /locus_tag="VGM24_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4155247.1"
                     /translation="MAKKTIAAPALSTEEKFKEAARKVFTRKGYAATRTRDIAEEAGL
                     NLALLNYYFRSKEKLFGLIMAEKMQQFFGFMLPVINNASTTLEDKVEIIASNYIDLLL
                     ENPDLPFFILTEIRNNPAYFSKITGKQNPLLKSVLIKQFQEKVPGRNPIHYLINLLGM
                     CVFPFVMNPVLQKQGVLDDKSFKALARERKTLIPVWIKTMLKSA"
     gene            1466..>1787
                     /locus_tag="VGM24_07465"
     CDS             1466..>1787
                     /locus_tag="VGM24_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4155248.1"
                     /translation="MGLLPAFLLLTFAAQAQTGPLTIDSCYRLAKENYPLIKKQDLIS
                     KSSGYSLENAARFFLPQVTVSGQASYQSQTIDFRDVLPGVPAGSLPNISKDQYRIQAE
                     VSQQI"
BASE COUNT          504 a          410 c          405 g          468 t
ORIGIN      
        1 ttatgtatat aataataatg aacgcagtat tctcccggtt taagaaggaa ttttcccctg
       61 cccacggatt tccgaaagga gtgttccatc aaatactgaa ggggcttttc cccgacctgg
      121 tagattgtct tcataaaatc caccagcggt gaaccgggta tctttgactt catctgggat
      181 taaaattggc accaaatata aaaaaatcct tccagttgcc cctgattcag ggctcattcc
      241 agcggaatga tccaatattc agatgctgcg tggggagaaa agcgtcctat tgcttgacaa
      301 attccctgac ctgccgtgaa ccgtccgaca attccaaaat agccgtatag gcgccccgcg
      361 ggaaatcacc gatattgatc gcgtagctgg aaggcaaaat tgaattgacc gcatacacca
      421 gaacactgtt gatcgaatag actttcaggc tattgacggt gatcaggctg ctgccggtgt
      481 tgagatataa gatggaagtg gcggagttcg ggtaaatagt taacaaggtt gaatttagaa
      541 tgggtatttg caatattaag catccctccg ttttggtctg agccgtcagc ccgttttatt
      601 gacaattatc aatacaattc tgataaccat caggctttcc gcttaggggt ttaccctgat
      661 tccttacctg gttttgtatg ttttttcgaa aagccttccg gaaacagttt aatcaattga
      721 ttaaataatc tgattaatat gaatatttgt ttctaccttt ggtccggaaa acaaaccggg
      781 atcccatggc aaagaagaca atcgcggcgc ccgctctttc caccgaagaa aaattcaagg
      841 aagccgccag aaaagtattt acccgtaaag gctatgcggc aacgcgcacc cgggatattg
      901 cagaggaagc cggattgaac ctggccctgc tgaactatta tttccggagc aaggaaaaat
      961 tgttcgggct gatcatggcg gagaaaatgc agcagttctt tggttttatg ctgccggtca
     1021 taaataatgc gtccactacc ctggaggata aagtggagat cattgcttcg aattacatcg
     1081 atctgctcct cgaaaacccg gaccttcctt ttttcattct caccgaaata agaaacaatc
     1141 cggcctattt ttcaaaaatc actggcaagc agaatccgct gctgaaatcc gttttgataa
     1201 aacaattcca ggaaaaagtg cccgggcgaa accctatcca ctacctgatc aatctcctgg
     1261 gcatgtgcgt gtttccattt gtcatgaatc cggttttaca aaaacagggg gtcctggacg
     1321 ataaatcatt taaagcattg gcaagggaaa gaaaaaccct gataccggtc tggataaaga
     1381 cgatgctgaa atctgcctga tttcctgccc ggatcaattc aataatataa atgtattcgc
     1441 tatgatgatt aaagcgggta agaggatggg gctactaccg gcctttcttt tactaacctt
     1501 cgccgctcag gcgcaaaccg gtccactgac cattgattcc tgttaccggc tggcaaaaga
     1561 aaattatccg ctgatcaaaa agcaggatct gatttcaaaa tccagcgggt attctctgga
     1621 aaatgcggcc aggttctttc ttccacaggt aacggtgagc ggtcaggcga gctaccagtc
     1681 ccaaaccatc gatttccggg atgtgcttcc cggcgtgccg gcgggctcat tgcccaatat
     1741 cagcaaggat cagtatagga tacaggcgga ggtcagtcag caaatct
//
LOCUS       DASYCU010000369         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2690596, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000369 DASYCU010000000
VERSION     DASYCU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690596"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VGM24_07470"
     CDS             <1..134
                     /locus_tag="VGM24_07470"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY4155249.1"
                     /translation="GCSGARIIVTLINILIQRNAVYGAAGICNGGGGASAMVIERLS"
     gene            complement(129..1241)
                     /locus_tag="VGM24_07475"
     CDS             complement(129..1241)
                     /locus_tag="VGM24_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEY4155250.1"
                     /translation="MAIINYQNFTIGIEEEYMVIDPETRELKSHEQKIVAEGQKVIRD
                     KVKAEMHQAVVEMGTDICEDIQAAHKDVSLLRKTISQIAGDLGLWVGASGTHPFSHWE
                     SQLITDHARYNEIVNELQEAARSNLIFGLHVHVGMESREMANHIANSTRYFLPHIYAL
                     STNSPFWEGRSTGYKSYRTKVFDKFPRTGIPEYFESIEAYENFVKLLVKTNCIDNAKK
                     IWWDLRVHPFFNTVEFRICDVPLTVEETICIAALFQAVCAKIYKLKTQNLNFIIYQRA
                     LINENKWRASRYGIDGHLIDFGKEEEVNTRALIYELLDFIDDVLDPLGSREAVSIIPK
                     IFETGTGADRQLKVYEEKKSLVDVVDYIHDQFLAGL"
     gene            complement(1274..2230)
                     /locus_tag="VGM24_07480"
     CDS             complement(1274..2230)
                     /locus_tag="VGM24_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155251.1"
                     /translation="MKKIGILYGKERSFPMAFIEAVNGRNVRGIQAEPVYIDKVIQGE
                     PSGYAVIIDRISQDVPFYRAYLKNAALSGTAVINNPFWWSADEKFFNNALATKIGVPV
                     PKTVILPSRELPEETSEHSFSNLAYPLDWESIFRYIGFPAYMKPFAGGGWKSVYRVSS
                     AEDFYQKHSETGRLVMLLQEEIVFEEYYRCYCIGGKHVRIMPYEPRNPHHLRYAAGFA
                     PPEERVRQMEQIVLRINQFLGYDFNTVELALRNGIPYAIDFCNPAPDAEKSSVGEDNF
                     NWVVETSASYAIERALETRDGLDNLTWGEYLKKTTLRKPLMS"
     gene            complement(2255..2968)
                     /locus_tag="VGM24_07485"
     CDS             complement(2255..2968)
                     /locus_tag="VGM24_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY4155252.1"
                     /translation="MNMKEEYHKWHSPSLDRDVEMLVFGYAGQPVILFPTSQGAYYQN
                     KDEGMIEAAKWFVENGKVKIYCPDTLDAQTWYNKSVSPEIRAHNYECYDRMLQTELIP
                     WAQHETGFERMALAGCSFGGYHAVNTSFRHPQLVSYCFSMSGAFDIRSFVSGFYDDTV
                     YFNNPVDFIVSDNNPNLWKMGIVLGTSDHDICKVENERLSSILHLKNINHWLDVRPNA
                     SHDWPVWREMFPHYLSLMS"
     gene            3051..>3394
                     /locus_tag="VGM24_07490"
     CDS             3051..>3394
                     /locus_tag="VGM24_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY4155253.1"
                     /translation="MQSVKPETVLKKSVTIPSAALGRSVAVDFYLPPPAGPGPVSVLL
                     INDGQDLARMKFQPLLEQLYGSEAIRPLLCIGIHAGTQRKLEYGTAHAPDYRSRGIYA
                     PRYTYFVIRELIP"
BASE COUNT          805 a          859 c          832 g          898 t
ORIGIN      
        1 tgggctgcag cggtgcgcgc atcatcgtaa ccctgatcaa tatattaatt cagagaaatg
       61 cggtatacgg cgcggccgga atctgtaacg gaggaggggg agccagtgcc atggtcatcg
      121 aaaggctgtc ataatccggc gagaaactga tcgtggatat aatccaccac atcaaccagg
      181 ctcttctttt cttcataaac ttttaactgg cggtctgccc ccgttccggt ttcgaaaatt
      241 tttgggataa tgctgacggc ctcccggctt cccagcggat ccagcacatc atctataaag
      301 tccagcaatt catagatcag ggcgcgggta ttcacttctt cttcttttcc aaaatcaatg
      361 aggtgcccgt caattccata gcggctggcg cgccatttat tttcattgat cagcgcccgc
      421 tggtagatga tgaaattcag attctgggtc ttcaacttat agatcttggc gcagacggcc
      481 tgaaacaggg ccgcaataca aatcgtttct tcaaccgtca gcggaacatc gcatattctg
      541 aattcgacgg tattgaagaa aggatgaacc cgcaggtccc accagatctt ttttgcgtta
      601 tcaatgcaat tggtcttcac cagcagcttg acaaaatttt catacgcttc aatgctttca
      661 aaatattcgg gaataccggt gcggggaaat ttatcgaaca ctttggtgcg gtacgacttg
      721 tacccggtgc tccggccttc ccagaacgga gagttggtgc tcagcgcata aatgtgcggc
      781 aggaaatagc gggttgaatt cgcgatatga ttggccattt cccggctttc cataccgaca
      841 tgaacatgca ggccgaagat cagattgctg cgggcggctt cctgcagttc gttgacgatc
      901 tcgttatacc gggcatgatc ggtgatgagc tggctttccc agtgactgaa gggatgcgtg
      961 ccggaggcgc cgacccagag acccagatcc cctgcaatct gcgaaatggt ttttcttaat
     1021 aaggaaacgt ccttgtgggc agcctgaata tcttcgcaga tatccgttcc catttccacc
     1081 accgcctgat gcatttccgc ctttactttg tcgcggatca ctttttggcc ctccgccacg
     1141 atcttttgct catggctttt taattcccgg gtctccgggt caatgaccat gtattcctct
     1201 tcgatgccga tcgtgaaatt ctgatagttg atgatagcca taggttgcaa gaggaatttg
     1261 gtggggtgct gaatcagctc ataaggggtt tccggagcgt cgtttttttc aggtactctc
     1321 cccaggtcag attatccaat ccgtcccggg tttccaaggc gcgttcaatc gcataggagg
     1381 ccgaggtttc aaccacccag ttgaaattat cttcccccac actggatttt tccgcatccg
     1441 gtgcgggatt gcaaaagtca atggcatacg gaatgccatt gcgcagggct aattccaccg
     1501 tattgaaatc ataccctaaa aattgattga tccttaatac gatttgttcc atctgcctta
     1561 ctcgttcttc cgggggcgca aaaccggctg cataccggag gtggtgcgga ttgcggggtt
     1621 cgtacggcat gatgcgtacg tgtttgccgc cgatgcagta acagcggtaa tattcttcaa
     1681 aaacaatttc ttcctgcagc aacatgacca ggcggccggt ttcactgtgt ttctggtaaa
     1741 aatcctctgc gctgctaacc cggtaaacgc tcttccatcc gcctccggca aaaggtttca
     1801 tataggcggg gaatccaatg tatctgaaaa tggattccca atcgagagga taagccagat
     1861 tggagaaaga atgttcggat gtttcttccg ggagttcccg ggaaggcagg ataacggtct
     1921 tgggaaccgg cacgccgatt tttgtggcga gcgcattatt gaaaaatttt tcatcggcgc
     1981 tccaccagaa cggattattg atcacggcgg tcccgctgag ggcggcattt ttcaggtaag
     2041 cgcggtagaa aggcacatcc tgcgaaatgc ggtcgataat taccgcataa ccgctcggct
     2101 caccctggat caccttgtcg atatacaccg gctccgcctg aattccgcgc acatttctgc
     2161 cgttcaccgc ctcaatgaaa gccatgggaa agcttctttc ttttccgtat aggatgccga
     2221 tctttttcat tgcggtgttt atgatttagg gaagttaact cattagagag agatagtgcg
     2281 gaaacatttc ccgccatacc ggccagtcat gactggcatt tggacgcaca tccagccagt
     2341 ggttgatatt cttaagatgc aggatgctgc tcaatctttc attctctact ttgcagatat
     2401 cgtggtcgct ggtgcccagc acgattccca ttttccacag gttgggatta ttgtcgctca
     2461 caatgaaatc caccgggtta ttaaaataga ccgtatcgtc ataaaatccg ctcacaaaag
     2521 aacggatatc aaaagcgccg ctcatggaaa aacaatagct gaccagctgc gggtgacgga
     2581 aagaagtatt taccgcatgg tagccgccaa aactgcagcc cgcaagggcc atccgttcaa
     2641 aacctgtttc gtgctgggcc caggggataa gttcagtctg cagcatccgg tcgtagcatt
     2701 cataattatg tgcccgtatt tccggagaaa cggatttgtt ataccaggtt tgggcatcca
     2761 gcgtatcggg acaatagatt ttcaccttgc cgttttccac gaaccatttt gcggcctcaa
     2821 tcattccctc gtctttattc tggtaatagg cgccctgtga ggtgggaaat aaaataacgg
     2881 gctgaccggc ataaccaaat accagcatct ccacatcccg gtcaagcgaa ggcgagtgcc
     2941 atttgtgata ttcttctttc atgttcatga acgccgggtt ggtgtgccat ccaaatttat
     3001 ttttaaaatg ctataaaaac aaatcagtat tctttattat ttttggattt atgcaatccg
     3061 taaaaccgga aacggtgttg aaaaagtcgg taacgattcc ttccgcagca ctgggaagaa
     3121 gcgttgcggt cgacttttac cttccgcccc cggccgggcc gggtcccgtt tccgttctgc
     3181 tgatcaacga cggccaggac ctggccagaa tgaagttcca acctctcctg gagcagttat
     3241 acgggtcgga agccatccgg cccctgcttt gtattggcat acatgcggga acccagcgta
     3301 aactggagta cggaacagcc catgccccgg attaccggag caggggcatc tatgcccccc
     3361 ggtatactta ttttgtgatc cgggagctca ttcc
//
LOCUS       DASYCU010000370         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1966764, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000370 DASYCU010000000
VERSION     DASYCU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966764"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VGM24_07495"
     CDS             complement(<1..231)
                     /locus_tag="VGM24_07495"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY4155254.1"
                     /translation="MKNLDFILSASLTLMSGMVTAQRPEKTSGIMYAGTSPGRGSKGI
                     YVLQFDPEQKKFTELQTVTEKKNPNFLAVSPDK"
     gene            complement(218..1939)
                     /locus_tag="VGM24_07500"
     CDS             complement(218..1939)
                     /locus_tag="VGM24_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4155255.1"
                     /translation="MILPPGDPDNGGLILPGGFEALVVADSIGRARHLAVNDNGDIYV
                     KLTYNDAMKGRGGTVGLRDTNNDGKADSIVYFGDYTDVGSSAVGVRIHNGYLYTSTVT
                     SVLRNKLRSGELVPESKTEVIMNDNRERVEHNWHTTKPVAFDNDGNMYVPFGAPTNAG
                     QDVSLYGPTGIPGGHGIDPDPELSEYAGIWRFDANKTGQTPKDGHQFATGIRSVVGMT
                     WNPGDNHLYLVMNGIDNFHTLYPKEYSSWQAAVLPAETLLKVTEGANYGWPYAYYDQM
                     LGKNMLEPGYGGDGKKVGRAAKFDLPLMGFPGHWAPMDLLFYQGDQFPERYKKGVFIA
                     FHGSTDRSPYPQAGYIVCFAPFVNGKPTGQYEVFADGFTGVDTVANTSDAKYRPMGLS
                     EGPDGSLYISESNKGKIWRVMYKGDKTKFGKEQLAELEKRKLTRTYIKTPDSVKDDLH
                     KGSEMEGGILFNTYCATCHQRNGMGDNNRYPPLAGSEWVSGDKERLMKIVLNGLQGAV
                     EVNGKTYNGLMPAHGGFLDDNAVASILTYIRKGRRFKNASAPIGPLEVARVRNISAKK
                     NRINEKP"
     gene            2255..>3663
                     /locus_tag="VGM24_07505"
     CDS             2255..>3663
                     /locus_tag="VGM24_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155256.1"
                     /translation="MPTSKKNHGTDRNSRRRFLKNTLTASAGFYIVPRHVLGRGFTAP
                     SDKLSIAAIGAGGKGQDDIDHFFKSGKANIAYLCDVDDRQAVETRKRFPDAKYYKDFR
                     EMLSKEAKHIDAVSVSIPDHTHAVAAMAAMQLNKHVYVQKPLTHDIYEARMLTQAAHR
                     YKVVTQMGNQGASGDGVRQLGEWLNAGLVGDVHTVYCFTDRPIWPQGIPRPATASPIP
                     AELNFDLWLSTAPQKSYFEGMLPFNWRGWWDYGTGALGDMACHIMAPAFTILELGYPV
                     SAECSVATKYIENWQVGYFPDSGPIASHITLTFKGKNGKPDVELHWMDGGIQPTRPEE
                     LAPNEQMGDGGNGAIFIGTKGKMMCGTYGIDPQLLPTSRTKTTKVPQTIARVPGGDEG
                     HYAAWIEAALAGYGSEKAKNLSSNFDIAGPLTESVLMGNLAIRSNNIQLKKGQDIEYP
                     GRHIKLLWDGPNMKITNFD"
BASE COUNT          902 a          940 c          886 g          935 t
ORIGIN      
        1 tttatccgga ctaaccgcca ggaaattggg atttttcttt tctgtcaccg tttgcagttc
       61 ggtgaatttt ttttgttccg ggtcaaactg aagtacatat atgcctttgc tacccctgcc
      121 tggggaagta cctgcataca tgatcccgga ggttttctcc ggccgctggg cggtcaccat
      181 cccgctcatg agggttaagg acgcagataa aataaagtca aggtttttca tttatcctgt
      241 tttttttagc ggaaatattt ctcactctgg caacttctaa cgggccgatc ggtgccgatg
      301 catttttaaa tcgcctgccc ttccggatat aggtcagaat ggaagctact gcattatcat
      361 ccaaaaaacc accgtgtgcc ggcatcaggc cattgtaggt cttaccgttt acttccacag
      421 cgccctgcaa tccgttcagg actattttca tgagcctttc cttatccccg cttacccatt
      481 cggatcctgc cagcggcgga tagcggttat tgtcacccat tccattccgc tggtgacagg
      541 tggcgcagta cgtattgaat aatattccgc cctccatttc actgccttta tggagatcgt
      601 cctttaccga atccggtgtt ttgatatagg tccgggttaa tttgcgtttt tccagttcag
      661 ccagttgttc ttttccaaat ttggtcttat ctcctttata catcacacgc cagatctttc
      721 ctttgttcga ttcggagatg tagagcgagc cgtcgggccc ttcagaaagg cccatgggcc
      781 ggtacttcgc gtcactggta ttcgcgaccg tatccactcc ggtgaagcca tccgcaaata
      841 cttcatactg accggttggc tttccgttta caaaaggcgc aaaacaaacg atgtatcctg
      901 cctgcggata aggggagcgg tctgtggaac cgtgaaatgc aatgaagacc ccttttttat
      961 aacgctcagg aaattgatca ccctggtaaa ataataaatc catcggagcc cagtggccgg
     1021 gaaatcccat cagcggaaga tcgaatttcg ccgcccgtcc gacctttttt ccatcccctc
     1081 catatccggg ttccagcata tttttaccca gcatctggtc gtaataagcg tagggccatc
     1141 cgtagtttgc gccctcggtc acttttaaca gggtttcggc gggaagtacg gccgcctgcc
     1201 aggagctgta ttcctttgga tataaagtat ggaaattatc aatcccattc ataactaaat
     1261 ataaatgatt atcgccgggg ttccaggtca tgcccaccac gctgcggatt ccggtcgcga
     1321 actgatgccc gtctttcggc gtttgtcctg ttttattggc gtcgaaacgc cagatgccgg
     1381 catattccga aagctccggg tcgggatcga tgccatggcc gccgggaatg ccggtggggc
     1441 cgtaaaggga aacatcctgg ccggcatttg ttggcgcgcc aaaaggaaca tacatgtttc
     1501 catcgttatc aaaagcgacc ggtttggtgg tatgccagtt atgttccacc cgttcccggt
     1561 tatcattcat gatgacttcc gttttgcttt ccggcacaag ttctccggag cgcagtttat
     1621 ttctcagcac gctggtcacc gtactggtat agagataccc gttgtgaatc cgaaccccaa
     1681 ccgctgaact tccgacatcg gtgtaatccc cgaaataaac aatggaatct gccttcccat
     1741 cgttattcgt gtcccggagt ccaaccgttc caccccttcc cttcatggca tcgttatacg
     1801 tcaatttcac atagatatca ccgttatcat tcacggccag gtgccgggcg cgtccaatgc
     1861 tatcggcgac gaccagcgct tcaaaaccac ccggaaggat caggccgccg ttatccggat
     1921 ctcccggcgg aaggatcacc ggtccccggc atgaggttag tgcggcaata gctaaactta
     1981 accagattac accggtaaat cgttctggcc ttttaaacgg ttgaataaaa tttataaaac
     2041 tgatttcaat ggaagaccgg aaacgcattg gctgtcgatt gcttttttgt tgcgccgatc
     2101 ccgggatgat gtgatgtgta acggcggaga atttctcggg aataagcagt gataacaaat
     2161 gtaaattatt ttaggatcag caaaagggat cgttaataaa aatatttatt taccttaggt
     2221 catcattaac cagctttaat aaaaaaacga tttaatgcct acgtctaaaa aaaatcacgg
     2281 aacggaccgg aattcgcgtc gccgttttct taaaaatacg ctgactgcct ctgccgggtt
     2341 ttatattgtc ccccggcatg ttttgggaag aggattcact gctccgagtg ataaactcag
     2401 cattgctgcc attggcgccg gcggaaaagg ccaggacgat atcgatcatt tttttaaaag
     2461 cgggaaggcc aatatcgctt acctctgcga tgtggatgac cggcaggccg tcgaaacccg
     2521 caagcgtttt cccgacgcca aatactacaa ggacttccgg gagatgcttt ccaaagaagc
     2581 gaaacacatc gatgcggttt cggtatccat tccggatcat acccatgccg tggccgccat
     2641 ggccgccatg caactgaata aacacgtgta tgttcaaaaa ccgctgacgc atgatattta
     2701 tgaagcccga atgctgaccc aggcggcgca tcgttacaag gtggtaacgc aaatgggtaa
     2761 tcagggcgcc tccggtgatg gtgtccgtca actgggagaa tggcttaatg ccgggcttgt
     2821 gggggatgtg cataccgtat attgttttac cgaccgcccc atctggccac agggaatacc
     2881 aagacctgcc accgcttcgc ccattcctgc ggaactgaac tttgacctgt ggctcagcac
     2941 ggctcctcaa aaaagctatt ttgaaggaat gcttccgttt aactggcgtg gctggtggga
     3001 ctatggcacg ggagctctcg gcgatatggc ctgtcacatc atggcgccgg cctttacgat
     3061 attggaactc ggatacccgg tctccgcgga atgcagcgtg gccacaaaat atattgaaaa
     3121 ctggcaggtg ggatattttc cggacagtgg tccgatcgct tcccatatca cccttacgtt
     3181 taaaggaaag aacggaaagc ccgacgtcga actgcactgg atggacggcg gcattcagcc
     3241 gacccgcccg gaagaactgg ctccgaatga gcaaatggga gatggcggaa acggcgcgat
     3301 ctttatcgga acaaaaggaa agatgatgtg tggaacctat ggtatcgatc cgcaattatt
     3361 accaacctcc agaacaaaaa caacaaaagt tccacagact atagccaggg taccgggcgg
     3421 ggatgagggc cattatgctg cctggataga agcagccctt gccggttacg gaagtgaaaa
     3481 ggcaaaaaac ctcagttcca atttcgatat cgccggaccc ttaacggaaa gcgtgctcat
     3541 gggtaatctc gccatccgga gcaataatat ccagcttaaa aaaggacagg atattgaata
     3601 tccgggccgt catatcaaac tcctttggga cggacctaat atgaagatca ccaatttcga
     3661 cga
//
LOCUS       DASYCU010000371         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4431966, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000371 DASYCU010000000
VERSION     DASYCU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4431966"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VGM24_07510"
     CDS             complement(<1..1029)
                     /locus_tag="VGM24_07510"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155257.1"
                     /translation="MKIHKILTAVLFVGLPAIFSGCKKSLQEKPYSFYNETQILKTAD
                     DARNLLLGAYNPLSNDYNMLRLPYWIAIDLDNDHITGPSWLFGNTGSGDYVDFWGMNG
                     IWLGHYQIVGRCNEVLAKVPPLDIDETIKNNILGEAHALRAWAYFSLVRMYGEVPIKM
                     KTLETDPEFNTPRSSVKDIYDFIIQDLNDANTLLLPKDDPKGGGPGRINKAAAQALLA
                     KVYLTMASGAQKGASLTVRGGKDNGYYTHTQNGVAGYEGFNPDSCFMQARDIAAGIMD
                     GSGGGGQFSLVPAFKDVFSKAQIDGPEAIWYLEFKDGSNTVNPLNMYYSPTSVKRGYG
                     GWAWLTDNF"
     gene            complement(1078..4227)
                     /locus_tag="VGM24_07515"
     CDS             complement(1078..4227)
                     /locus_tag="VGM24_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4155258.1"
                     /translation="MLKPKRLLFQLLFIGFAQLLSQQVFSQTKIISGKITDAESGLPL
                     SGVTVMSKETKEATQTDSAGKFSLSVSQQARTLVFSYVGFENTEMPVGVQHYLAVAME
                     PGSKKLSDVVVVGYGSVKKSDITGALASVRGDELMKSNPVSMEQGLQGKVAGVQVLQN
                     DGAPGAGISVQIRGSNSFLGGTEPLYVVDGIPFNGDNSSVTPTALQPNEVQTINALSF
                     INPADIESIEILKDASATAIYGSRGANGVVLITTRKGRKGRDRIELNVVNTVSRVTHQ
                     IHMLDAETYAEYQNESYLNADKYAGTNYEATGQLPYTGTTDPVTGLYKPAPAEFRSKS
                     TNWQDEVFRTALTSDYNLSFSGGSDKGNHLLSFDYLNQQGTMVNSGYKRGSVRMNLSR
                     NVTSWLTVGSNTSLTRSLNNMLKTGSTDLFGEAGVIRSALTYPPVFGLNDLKIQRLTD
                     PYTYTRDVQNQVSSNSLFSSNYLEITLAKGLKIRQNIGLSYLGNERNQYYPVTTFEGS
                     NAHGKAGVSDNFTYSVVSESILSYQKKFKKHDFSFMGASTYEKTLLGFKATGASNFEN
                     DILTSFNLAAGSVYAVPENGRTQYSLNSFLGRINYSYDDKYLATVSYRADGSSKFAAQ
                     NKWAGFPSFALAWKINRESFLSNAGAVSDLKLRFSYGQTGNQAIPPYASLSKYTPVPF
                     IFNSTLYNGYAVNQPGNNDLRWETTAQFNLGLDLALRNIFSFTVDVYKKKTSDLLQNV
                     VLPGSTGYSSQLQNMGAVENKGMELGLNALIINEAFRWRASANFSLNRNKILSLGDKQ
                     RQFGANVTLNDAPFIQQVGSPIGLLYGYVEDGFYNSEAEVKADPQYAGAPDDVVKKMV
                     GEIRYKDLDGVAGITDADRTVIGNTNPKFIYGFTNNFNYKNFDLSIFIQGSQGNDIIN
                     LNTYYMSNLGFPQYGNITQALYDGRWTPENAAHAVNPKAIATTDRSFKFSRRFIENGS
                     YVRIKNINLGYQLNFPENKYISSVRLFLSADNLLTITKYTGFDPDVNGYGQDPSLRGV
                     DLGGYPVSRSFALGVKCMF"
     gene            complement(4524..>4918)
                     /locus_tag="VGM24_07520"
     CDS             complement(4524..>4918)
                     /locus_tag="VGM24_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4155259.1"
                     /translation="ADSLVTGPNTIFAKMQTLDKCNQTGFSIDSITITKTSAGGMLDP
                     DFPNSPIGSYPNPVTDKIYVSGLSATKSYTIQIYNNQGAQVSQLSVNGQTGVNIYAGS
                     LQSGVYWLQVYDNSKNKKIGTTMLLKSR"
BASE COUNT         1151 a         1214 c         1202 g         1351 t
ORIGIN      
        1 aaaattatct gtaagccagg cccatccgcc gtatccccgt tttacactgg tcggggaata
       61 atacatattc aacgggttta ccgtattgct tccatccttg aattcgagat accagatggc
      121 ttcgggccca tcgatctgtg ccttcgaaaa tacatcttta aaagccggga cgaggctaaa
      181 ctgcccgccg ccgccggagc catccatgat tccggccgcg atatcccttg cctgcataaa
      241 acaggaatcc gggttaaacc cttcgtaccc ggccacgccg ttttgcgtat gggtataata
      301 tccattgtcc tttccgcccc ggacggtaag cgaagcgcct ttctgcgcgc ccgaagccat
      361 ggtcaaatag actttggcca gcagggcctg ggctgcggct ttgttgatcc ggcccggtcc
      421 gccgcccttg gggtcgtctt tcggcagaag cagggtattt gcatcgttca ggtcctgtat
      481 aataaaatcg tatatgtctt ttacggaaga tcgcggggta ttgaactccg gatccgtttc
      541 gagcgttttc attttgatcg gaacctctcc gtacattctc acgaggctga aataggccca
      601 tgcccgtaac gcatgcgctt ctcccagaat attattcttt atggtttcgt cgatgtcgag
      661 cggtggaact tttgccagca cctcgttgca tctccccacg atctgatagt ggcccagcca
      721 gatgccgttc attccccaga aatccacata atcccccgag cccgtattcc caaacaacca
      781 ggaaggcccg gtaatatgat cgttgtccag gtcgatggcg atccagtatg gaagccggag
      841 catattataa tcattcgaca ggggattgta ggcgccgagc aataagttcc gggcatcgtc
      901 cgccgttttt aagatctgcg tttcgttgta gaagctgtac ggtttttcct gaagcgattt
      961 tttacagccg gaaaatatcg ccggcaggcc tacaaaaaga actgccgtta aaatcttatg
     1021 tattttcatc ttgaatcctt tataaatgat ttaaactgtt tttacctgag ccgtctatta
     1081 aaacatgcat tttacaccga gtgcaaaaga tctcgatacc gggtagccac ccaggtccac
     1141 cccgcgcagg gaaggatcct gcccgtatcc gtttacatcc ggatcaaagc cggtatattt
     1201 tgtaatagtc aggaggttgt cggccgataa aaacaatctc acgctgctga tatatttgtt
     1261 ttccggaaaa ttaagctggt aacccaggtt aatatttttt attcgtacat aggacccgtt
     1321 ttctataaag cggcgggaaa acttaaagga acggtcggtg gtggcaatcg ctttcgggtt
     1381 taccgcgtgt gcggcatttt ccggagtcca ccggccgtca taaagcgcct gggtaatatt
     1441 tccgtactgg ggaaatccga gattgctcat ataataggtg ttcaggttaa tgatgtcgtt
     1501 cccctgagaa ccctggatga aaatactcag atcaaaattt ttataattga agttgttcgt
     1561 gaagccataa atgaatttcg ggttggtgtt cccgatcacg gtacgatccg catcggtaat
     1621 gcccgccaca ccgtccaggt ccttatatcg tatttcgccc accattttct ttacgacgtc
     1681 atccggtgcg cctgcgtatt ggggatccgc cttcacttcc gcttccgaat tgtaaaatcc
     1741 gtcctccaca tacccgtaca acaaaccgat cggtgagccc acctgctgaa taaacggcgc
     1801 atcattcagg gtaacattcg cgccgaattg ccgctgcttg tctccgagag agaggatctt
     1861 gttccggttg agtgagaaat tcgcagacgc tctccatctg aacgcctcgt tgatgatcag
     1921 cgcgttcagg ccgagttcca tgcccttgtt ttccacggct cccatattct gcaattggga
     1981 agaatagccg gtactgccgg gcagcaccac gttctgcagc aggtcgctgg ttttcttttt
     2041 atagacatca acggtgaatg aaaaaatatt ccggagcgcc aggtcgagtc ccaggttaaa
     2101 ctgtgcggtc gtttcccatc tcaggtcgtt attgcccggc tggttgactg catacccgtt
     2161 ataaagcgtg ctattaaaaa taaaaggaac gggcgtgtat tttgacaggg aggcgtaggg
     2221 cggaatggcc tggttacctg tttgaccata gctaaagcgc agtttcagat cgctgacggc
     2281 gccggcatta ctgagaaagc tttccctgtt tattttccag gccagggcaa aggaaggaaa
     2341 gccggcccat ttattctgcg cagcaaattt cgaagaaccg tcggcgcggt aggacacggt
     2401 agccaggtat ttatcatcat agctgtagtt gatgcggccg agaaaggaat tcagcgaata
     2461 ctgcgttctc ccgttttccg gaacggcgta aacgcttccg gcggcaagat taaaactcgt
     2521 taaaatatca ttttcaaaat tgctcgcacc ggttgcttta aagcccagca gggttttttc
     2581 ataggtgctg gcgcccatga aagagaaatc gtgttttttg aatttcttct ggtaagaaag
     2641 aatgctttca gataccacgc tgtaggtaaa attatcggag actccggcct ttccgtgggc
     2701 attgcttcct tcaaacgtgg ttacgggata atattgattc ctttcgttgc cgaggtagct
     2761 caggccgata ttctgtctta ttttcaaccc tttggccaaa gtaatttcca gatagttgga
     2821 ggaaaacagg ctattcgaac tgacctggtt ctgtacgtcc cgtgtatagg tatacgggtc
     2881 ggtcagacgc tgtatcttga gatcgttcaa tccgaaaacc ggcggataag tcagcgcaga
     2941 gcggatgacg ccggcctctc cgaagagatc cgtgctgccg gttttcagca tattgttgag
     3001 ggacctggtt aatgaagtat tcgatcccac cgtaagccag cttgttacgt tccgggataa
     3061 attcattctt acgctgccgc gcttatatcc tgaattgacc atggttccct gctgattcag
     3121 gtagtcaaaa ctgagcaggt gattgccttt gtccgagcct cccgaaaatg acaggttata
     3181 atcggaagtg agggccgtgc ggaaaacttc atcctgccag tttgtgctct tcgatctgaa
     3241 ttccgcgggc gcgggtttat aaagaccggt aaccggatcc gtggtgccgg tatagggaag
     3301 ttgtccggta gcttcataat ttgttcccgc atacttatcg gcattcagat aggattcatt
     3361 ctgatattcg gcatacgttt ccgcatccag catgtggatc tgatgtgtta cccgggaaac
     3421 cgtattcacc acgttaagtt ctatccggtc gcggcctttt ctcccctttc ttgtggtgat
     3481 cagaacgacg ccgttggctc ccctggatcc gtagatggcg gttgccgatg catctttcag
     3541 aatttctatg gattcaatat cggccgggtt gataaaggat aaggcattga tcgtttgtac
     3601 ttcgttcggc tgcagagcag tcggcgtaac ggaactgttg tctccgttaa acggtatgcc
     3661 gtcaactacg tacagcggtt cggttcctcc caaaaaagaa ttggatccac ggatctggac
     3721 gctgataccc gcgccgggtg cgccgtcatt ttgcaggacc tggacgccgg ccacttttcc
     3781 ctgtaatccc tgctccatgc tgaccggatt agatttcatc agttcatctc ctctcacgga
     3841 agccagcgca ccggtgatgt ctgatttctt aaccgacccg taacccacga ccaccacgtc
     3901 ggagagtttt ttacttccgg gctccatcgc tacggccaaa taatgctgta cgcccaccgg
     3961 catctccgta ttttcaaatc ccacgtaaga gaaaacgagc gttctggcct gctgagaaac
     4021 agagaggctg aattttccgg cactgtctgt ctgcgtggct tctttcgttt ccttcgacat
     4081 gacggtgacc ccgctcaagg gaagaccgct ttccgcatcc gtaatttttc cgctgattat
     4141 ttttgtttgc gaaaagacct gctgcgagag cagctgagca aatccaatga acaataattg
     4201 aaagagcaat cgttttggct tcaacatttt aaatggaggt tattgatgaa taaaaaaggc
     4261 gagagagagg agaattgcgg gcaaaccctg gcctgctccc tttttccagg cagttcgtcc
     4321 cttgaccgta tcaaaaatta aattgggttg tcagcgcagg aaggttaccg tcaacatcga
     4381 tccatccctc ttattttgta caaagtaagt gtatagaaaa ccggaaatct ttatcaaaat
     4441 tttccgaacc tgataaatta ttttccattt tcagcgtttg cggacggaat gaggagaagg
     4501 tttgggatgc cggggcggtt ttcctaacgg gacttcaaca gcatagtcgt tccgatcttt
     4561 ttgtttttgc tgttgtcgta aacctgcaac cagtaaacgc cggattgaag agagccggca
     4621 tagatgttta ctccggtctg accgttcacg cttagctgtg aaacctgcgc accctggttg
     4681 ttatagattt gaatcgtata agatttcgtg gcacttaacc cgctgacata tatcttatcg
     4741 gtaaccggat tgggatagct gccgatgggc gaatttggaa agtcaggatc cagcataccg
     4801 cctgcgctgg ttttcgttat cgtaatgctg tcgatcgaaa atccggtctg gttgcattta
     4861 tccagggtct gcatttttgc aaaaatggta ttcggaccgg taaccaaact atccgcag
//
LOCUS       DASYCU010000372         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3561644, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000372 DASYCU010000000
VERSION     DASYCU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3561644"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..>1501)
                     /gene="creD"
                     /locus_tag="VGM24_07525"
     CDS             complement(92..>1501)
                     /gene="creD"
                     /locus_tag="VGM24_07525"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEY4155260.1"
                     /translation="QAYFSAPDHLTIKKHTNMETVNTPDRVWENSRPTRSNKILYKGI
                     TTGVLILVMMIPTVFITDLVKERQQRQEEVAKEVSSRWALGQTLTGPYIYLPYKTTYL
                     DQDNKPKEQTLHLLVIPDNLNVTGRIDHELRQRSIYKVLLYRASIADTGDFVLQIPKE
                     IQADQVQWNDARICYGLSDFKGIEERLVIQFNGAPYELSPGLPVNDINEKGLSASIPL
                     SVADLGKSISFHIGLKIKGSEMLHFVPFAGNSRFTLTSAWPDPSFDGGNLPTERTVSD
                     QGFSAKWAFNKANLPFGTVLKDFKFDQSALAFGVTMVQPADEYAKTNRSVKYAILIIG
                     LTFSIFFIVELMQKKPIHPIQYILIGLALVIFYSLLLAISEFVLFDTAYLVASLATIG
                     LISLYARSHFRNWKTAGIFGGVLTLLYGFIFVLIRLEDTALLIGSIGLFIILALTMYA
                     SKKINWYGLDSSTAVLQQE"
BASE COUNT          370 a          339 c          381 g          411 t
ORIGIN      
        1 gctgtttttt gtttcatttc aaaatggaat aaatgattta ctgtttatat ccgccggtgc
       61 ttccggtttc cggtaaccgg cggaagagaa attattcctg ctgaagaaca gccgttgatg
      121 aatccaggcc ataccagtta atttttttgc tcgcgtacat ggtcagtgcc aggattatga
      181 aaagtccgat gcttccgatc agcagggccg tatcttccag ccggatcaga acaaaaataa
      241 aaccatatag cagggtcagg acgcctccaa atataccggc tgttttccag tttcgaaaat
      301 ggcttctggc gtacaggctg atcaggccga tggtggccag ggaggcaacc aggtaggccg
      361 tatcgaacag gacgaattcg ctgatcgcca ggagaaggct gtagaagatc accagggcca
      421 gtccgatcaa aatgtattga atgggatgga tgggtttttt ctgcatgagt tccacaatga
      481 agaatatcga gaacgtcaat ccgatgatca ggatcgcata ttttacgctc cggtttgttt
      541 tggcatattc atctgccggt tgcaccatgg tgactccgaa agccagggcc gactgatcaa
      601 atttaaaatc tttcaatacg gttccgaagg gcaggttggc tttgttgaaa gcccatttgg
      661 cgctgaatcc ctggtccgaa accgttcttt ccgttggcag gttgccgccg tcaaaagaag
      721 ggtcgggcca ggccgatgtc aacgtgaagc ggctgttccc ggcgaatgga acaaaatgca
      781 gcatctcgct tcctttgatc ttaagaccga tatgaaagga tatggatttg cccagatcgg
      841 caacggataa gggaatcgaa gcggataacc ccttttcatt gatatcattc accggtaagc
      901 cgggagataa ttcataggga gcaccattaa actggatgac cagtctttcc tcaattccct
      961 taaagtcgga aagcccgtaa cagatcctgg catcattcca ttgaacctga tccgcctgaa
     1021 tttcttttgg gatctgcaac acaaaatcgc ccgtatcggc tatggatgcc cggtataaaa
     1081 ggactttata aatggatcgt tgacgcaatt catgatcgat gcgtccggtt acattcaggt
     1141 tgtccggaat gaccagcagg tgaagggttt gctcctttgg tttattgtcc tggtcaaggt
     1201 aggtggtttt ataaggcagg tagatatagg gaccggtcag cgtttgcccc agggcccagc
     1261 ggctgcttac ttcctttgcc acctcctcct gtctttgttg ccgctccttt accaggtccg
     1321 tgataaatac ggtgggaatc atcatcacca ggataagtac cccggtcgta attcctttgt
     1381 aaagaatctt gttcgaacgg gttggtctgc tgttttccca aacccggtca ggcgtgttga
     1441 cggtttccat atttgtgtgt ttttttattg ttaaatgatc aggggctgaa aaataggcct
     1501 g
//
LOCUS       DASYCU010000373         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_661967, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000373 DASYCU010000000
VERSION     DASYCU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661967"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VGM24_07530"
     CDS             <1..389
                     /locus_tag="VGM24_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY4155261.1"
                     /translation="GTITDASGRTLSGQTLEAFYTSIAHAKPISVGLNCALGAAEMRP
                     HIEELSRLAVCYTSAYPNAGLPNSMGAYEEEPEETAHFLEEWAKEGFVNIVGGCCGTT
                     PEHIRHIAEKVKRFDPRKLPVVETAA"
     gene            400..3147
                     /gene="metH"
                     /locus_tag="VGM24_07535"
     CDS             400..3147
                     /gene="metH"
                     /locus_tag="VGM24_07535"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY4155262.1"
                     /translation="MTEENIIRPWLRLSGLEPLVVRPESNFVNIGERTNITGSKKFAR
                     LIRENRYEEALSVARQQVENGAQILDVNMDDALLDGEKAMTNFLNLIQSEPDIARIPI
                     MIDSSKFNIIEAGLKCVQGKCIVNSISMKEGEEKFIEQAIICKSFGAAVVVMAFDEKG
                     QADTKQRKIDISHRAYEILTRQLNYDPQDIIFDLNIFAIATGLEEHNNYAVDFIEAAK
                     EVKGQHPLVKLSGGVSNLSFSFRGNETVREAMHSVFLYYAIRAGLGMGIVNAGQMIVY
                     DEIDPVLRDLCENVILNRNNDNNEATDKLLAFAETVKRKGKEEKKDETWRKTPVEERL
                     KHALINGITDYIDADTEEARLLYPKPLDVIEGPLMDGMNVVGDLFGSGKMFLPQVVKS
                     ARVMKKSVAILTPYIEQEKLEKFNRRNAANTSGIATEEEKGPAKILLATVKGDVHDIG
                     KNIVGVVLGCNGYDIIDLGVMVSADRILETAIKEKVDIVGLSGLITPSLDEMVHVAKE
                     MKRRNFELPLMIGGATTSRMHTAIRIAPEYGHGVVHVLDASRSVTVAGTLLNETQKLP
                     FLEKLGLEYQKLKADFASKKSGKQLLGFKEAQANKTKIDWAKFEPVKPAFSGIKVFDH
                     PDLEEIARYIDWQPFFIAWEIHGKFPELLKDEKIGEAATKLYEDAQNLLRKIIGEKWL
                     TPRGTIGIWPANGTDADTITVQPQPGNAAPVNLECLRQQSKKAAGQANISLADFISPA
                     ETGKTDYIGAFSVTIQGIEKHIDRFMADQDDYNKIILQALSDRLAEAFAEMLHKKVRT
                     DYWGYEKEEQLTNEQLIKEEYKGIRPAPGYPACPDHTEKYKLFALLGGEENTGIQLTE
                     SLAMYPASSVCGWYFANPESKYFGIGKINKDQVTDYARRKQMEISEVERWLRPVLDYD
                     A"
BASE COUNT          930 a          795 c          849 g          670 t
ORIGIN      
        1 gcggcaccat taccgatgcc agcggaagga ccctcagcgg tcaaaccctg gaggcctttt
       61 acacctccat cgcgcacgcg aaaccgataa gcgtgggttt gaactgcgcg ctcggggcag
      121 ccgaaatgcg gccgcatatc gaagagctgt ccaggctggc ggtctgttat acttcggctt
      181 atccgaatgc gggcctgccc aattccatgg gcgcttatga ggaagagccg gaagaaaccg
      241 cccatttcct ggaagaatgg gcaaaagaag ggtttgtgaa tatcgtgggc gggtgctgcg
      301 gcaccacccc cgaacatatc cggcacattg cagaaaaagt aaaaagattc gacccgagaa
      361 aattaccggt tgtcgaaacc gcagcctaaa catcagatca tgaccgaaga aaatattatc
      421 aggccctggc tccgtttatc cggtctggaa ccgctggtcg ttcgccccga atccaatttc
      481 gtcaatatcg gagaacgcac gaatatcacc ggttccaaaa aatttgcccg gcttatccgg
      541 gaaaacaggt atgaagaagc gctcagcgtt gcccgccagc aggtggagaa cggcgcccag
      601 atcctcgacg tcaacatgga cgatgccctg ctcgatggcg agaaagccat gaccaatttc
      661 ctcaacctga tccagagcga gcccgatatt gcccggattc ccatcatgat cgactcgtcc
      721 aagttcaata tcatcgaagc gggactcaaa tgcgttcagg gaaaatgtat tgtcaattcc
      781 atttccatga aggaagggga agaaaaattc attgagcagg ccatcatctg taaaagtttt
      841 ggcgcggccg tggtggtgat ggcttttgat gaaaaagggc aggccgatac caaacagcgt
      901 aagatcgaca tctctcaccg ggcctatgag atcctgacca gacaattaaa ctacgatccg
      961 caggatatca tttttgacct taatattttc gccatcgcca ccggtttgga ggaacataat
     1021 aattatgccg ttgattttat cgaagcagcc aaagaggtaa aaggacaaca cccgctggta
     1081 aagctcagcg gcggagtgag caacctgtct ttctccttcc gcggaaacga aacggtacgc
     1141 gaagccatgc acagcgtatt tctttattat gccatccggg ccggcctggg catgggcatc
     1201 gtgaatgcag gccagatgat tgtttacgat gaaatagacc cggtgctccg cgatttatgt
     1261 gagaacgtga tcctgaaccg caataacgat aacaatgaag caacagataa attactggcc
     1321 tttgctgaaa cggtaaaaag gaagggcaag gaagaaaaaa aagacgaaac ctggcggaag
     1381 accccggtgg aagaaagact caaacacgcg ctgatcaacg gcatcactga ttatattgat
     1441 gccgatacgg aagaagccag attgttatat ccgaaaccgc tggatgtgat tgaaggaccg
     1501 ctgatggatg gcatgaatgt ggtcggcgat cttttcggaa gcggaaaaat gtttctcccc
     1561 caggtggtga agagtgcgcg ggtgatgaag aaatccgtag ccatcctcac gccctacatt
     1621 gaacaggaaa agctggaaaa atttaaccgc aggaacgcgg ccaatacgtc cggcatcgct
     1681 acggaagagg aaaaaggacc ggcaaagatc ttactcgcca ccgtaaaagg cgatgtgcac
     1741 gatatcggca agaacatagt aggcgtggtc ctgggttgca acgggtatga tatcatcgat
     1801 ctcggggtga tggtttccgc agaccggatc ctcgaaacgg ccataaaaga aaaagtggat
     1861 atcgtggggc tgagcggtct catcacgccc tccctcgacg aaatggtgca tgtggctaaa
     1921 gagatgaaaa ggcggaattt cgaattgccc ctgatgattg gcggcgccac cacctcgcgc
     1981 atgcacacgg ccatccggat cgcgcccgaa tacggacatg gcgtggtcca tgtgctggat
     2041 gcttcccgca gcgtaaccgt tgccggcacc ctgctcaatg aaacccagaa acttcctttc
     2101 ctggaaaagc tgggcctgga atatcaaaaa ctcaaagccg atttcgccag taaaaagtct
     2161 ggaaaacaat tgcttggttt taaagaagca caggccaata aaacaaaaat cgactgggca
     2221 aaatttgaac ccgtgaaacc cgcattttcc ggaataaagg ttttcgatca tcccgacctg
     2281 gaagaaattg cccgttacat tgactggcaa ccatttttta ttgcctggga gatccacgga
     2341 aaattccccg agctcttaaa ggatgaaaag atcggcgaag cagccacaaa actatatgag
     2401 gacgcacaga acctgctccg gaagatcatc ggcgaaaaat ggctgactcc ccgcggcacg
     2461 atcgggatct ggccggcgaa tgggacggat gccgacacga tcaccgttca gccccagccg
     2521 ggaaatgcgg ccccggtaaa cctggaatgt ttaagacagc aatcgaagaa ggcggcgggc
     2581 caggccaata tctcactcgc tgattttatc agcccggccg aaaccggaaa aacggattat
     2641 atcggcgctt tcagcgtcac catacagggg atcgaaaagc atattgaccg gttcatggcc
     2701 gaccaggacg attacaataa gatcatcctg caggccttgt cggaccggtt ggcggaagcc
     2761 tttgccgaaa tgctgcataa aaaagtgcgc acggattact ggggttatga aaaagaagaa
     2821 cagttaacca acgagcaact cattaaggaa gaatataaag ggatccgccc ggcgcccggc
     2881 tatcctgcct gcccggatca taccgaaaaa tataagctgt ttgcattgct gggaggggaa
     2941 gaaaataccg gtatccaact taccgaatcc ctggcgatgt atccggcttc ctccgtatgc
     3001 ggatggtact tcgctaatcc ggaaagcaaa tatttcggta tcggtaagat caataaagac
     3061 caggtgaccg attatgcgcg ccggaaacaa atggaaattt cagaagtgga gcgctggctc
     3121 cggccggtgc tggattatga cgcctgatgg taaataactg gttaaccggt ttgaaaccag
     3181 catctttgtt agtagctttg ccagtctttt taaaaatttt tatcctttat ggaaaaacga
     3241 aaag
//
LOCUS       DASYCU010000374         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4286768, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000374 DASYCU010000000
VERSION     DASYCU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4286768"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VGM24_07540"
     CDS             <1..577
                     /locus_tag="VGM24_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155263.1"
                     /translation="LLDQGIHLLDVCNWTLDEHALGAIGRGSNKGGPAVGDTWNNFQV
                     VYQYPNNVNVSIHSTQVGPYFGDVCCRFAGTKGIAEAHYSGGVFIHGDNEWDSGIAKA
                     ETELTPQQRAAGIFTSSLYDADANKEIAFIKSIETGNYLNGIRSGVESTLTAILGREA
                     ALTGKETNWNEIFQSNQKSDPKLTLSQFDKP"
     gene            589..1506
                     /locus_tag="VGM24_07545"
     CDS             589..1506
                     /locus_tag="VGM24_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY4155264.1"
                     /translation="MNRRKFIYSTALIPGLPRVLPAVARTGIKENKALAGDISIGIIV
                     GADNPEEDLKKVADLGFSACQISIKTYSPDLANRLDAALKKYQLTPVTLMCMGPGPYI
                     YNFMEGPSTIGLVPREYRAARVERLKQGIDFCKAASLPSVHAHFGFIPENPKDILYTE
                     FIDVMKGVAGYALKQNIALYFETGQETPITLRRAIEDIGTGNLFVNYDTANLVMYGKA
                     NPLDGLKILGPYIKSFHAKDGQYPTNPRELGKEVLIPEGEVNFPGIISFLKKMNFKGN
                     ITIECELSEKRKEYLLKTKEYLEKLIASV"
     gene            1640..1930
                     /locus_tag="VGM24_07550"
     CDS             1640..1930
                     /locus_tag="VGM24_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY4155265.1"
                     /translation="MSLQRIFTATLSGIVIGLLIAPAKGSETRQKIADSADQIREKIR
                     KIRGTTNQELEELHEVFEHEIDGLREDIRVKVLRLIEVSKKGYNHVKAEAMN"
     gene            complement(1948..>3000)
                     /locus_tag="VGM24_07555"
     CDS             complement(1948..>3000)
                     /locus_tag="VGM24_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEY4155266.1"
                     /translation="ITSGQGEKGFSWNDRYNMWSGMIGGFFLALSYFGTDQSQVGRYL
                     TARSTRESKLGLLLNGLVKVPMQFGILLLGTLVFAYYQFAREPLFFNQTQIELLSKTK
                     YADSLQIVLKEYDRLQKQKVEQMNPAGGTPVVNRMQEGLVMQNAQREEARLRVTIKRW
                     LDDKEVKGDSNDTNYIFLRFVIDHLPKGLIGLLIAVIFLSAWGSVAAALNALSSATIN
                     DIHKRWVKIPLTSKQEYQYSKWYTLAWGTFCVIVAQLAYNIGNSLIEAVNVLGSLFYG
                     VILGIFLVAFYLKNISATPVFISSLITETFVITIHILNQKNVVNISFLWLNAIGAAGV
                     LLLSVIIQKLKRADTG"
BASE COUNT          849 a          731 c          730 g          690 t
ORIGIN      
        1 attgctggac cagggaatcc acctgctgga tgtttgtaac tggacgctgg atgaacatgc
       61 gctcggggcc atcggcaggg gaagcaataa aggaggtccg gccgttgggg atacctggaa
      121 taatttccag gtcgtctacc agtatccgaa caatgtgaac gtgagcattc attcaaccca
      181 ggtcggcccc tattttgggg atgtctgctg ccggtttgcc ggaacaaaag gcattgcgga
      241 agcgcattac agcggaggtg tttttatcca cggcgataat gaatgggatt ccggaatagc
      301 aaaggcagaa accgagctga cccctcagca gcgggcggcc ggaatattca cctcttccct
      361 ttatgatgcg gatgccaata aggaaatcgc ctttataaaa agtattgaaa cgggaaatta
      421 cctgaacggg atccgttccg gagtcgaatc aacgctcacc gccatattgg gaagagaagc
      481 ggcgctgacc ggcaaggaaa cgaactggaa tgagatcttc cagtccaatc aaaaatccga
      541 tccgaagctg accctttctc aatttgataa accataagaa caataatcat gaacagaaga
      601 aaatttattt actccaccgc actgattccg ggattgccgc gcgtgctgcc ggcagttgcc
      661 cggaccggca ttaaagaaaa taaggcactg gcgggagaca tttccattgg tattatagta
      721 ggcgccgaca accccgaaga agatctgaaa aaagtagcag acctgggttt ttccgcctgc
      781 cagatatcca taaaaacgta ttcccctgat ctggcaaacc ggctggatgc ggcgctcaaa
      841 aaatatcagc tcactcccgt taccctcatg tgcatgggac ccggtcctta tatctataat
      901 tttatggaag gaccttcaac cattggtctg gtgccgaggg aatatcgggc cgcccgggtg
      961 gaaaggctga aacagggaat cgatttttgc aaggcagcct cccttccctc cgtacatgcc
     1021 cattttggat ttattccgga aaatcccaag gacattcttt ataccgagtt tattgatgtt
     1081 atgaaaggcg ttgccggata cgcactaaaa caaaacattg cactgtattt tgaaaccggg
     1141 caggaaaccc cgatcaccct ccgccgggcc attgaagata tcggaaccgg caaccttttt
     1201 gtgaactacg atacggccaa cctggtcatg tatgggaagg caaacccgct ggacggattg
     1261 aagatcctcg gcccttatat taaatcattt catgccaaag acggacaata ccctaccaac
     1321 ccacgcgaat taggcaagga agtccttata ccggaaggag aagttaattt tccgggaata
     1381 atttcctttc ttaaaaagat gaatttcaag ggaaacatca ccatcgaatg tgagttaagc
     1441 gaaaaaagaa aggaatatct cctgaaaaca aaggaatacc tcgaaaaact catagcatcc
     1501 gtataaaagc acgctatcct gccggtaacc cggaccggag caaaggggat ccggaacaac
     1561 ccggatccgg ctttcttatt ctggcaagat tgttgaaaat tatattctga taaacggcat
     1621 cattttaaaa tcatacgtta tgagtttaca aagaatattt acagcaacct tgtcagggat
     1681 cgttatcgga ttgttgatcg cgccggcgaa agggagcgaa accaggcaga aaatagcgga
     1741 ttccgccgat cagatcagag aaaagatccg gaaaatcagg ggaaccacca accaggaact
     1801 ggaagaactt catgaagttt ttgaacacga gatcgacggg cttcgggaag acatccgggt
     1861 aaaagtgcta cggttgattg aagtgagtaa aaaaggctac aatcacgtaa aggcggaggc
     1921 catgaattaa gaggggaaga atgaccgcta tccggtgtct gcccttttca gcttttgtat
     1981 tatcacgctc agcaacagca ccccggctgc gccgatcgca ttcagccaga gaaaactgat
     2041 gttcaccacg tttttctggt tgaggatgtg tatcgtgatc acaaacgttt ccgtgatcag
     2101 ggagctgata aataccggcg tggcagaaat gtttttgaga tagaaagcaa ccaggaagat
     2161 cccgaggatc acgccataaa ataaagatcc caaaacatta accgcttcga tcaggctgtt
     2221 cccgatatta taggccagct gggcaacgat cacgcaaaag gttccccagg ccagggtata
     2281 ccatttcgaa tactgatatt cctgtttgct ggtcagcggt attttgaccc accgcttatg
     2341 gatgtcgttg atcgtggcgg aggacaacgc attgagcgcc gccgccaccg agccccaggc
     2401 ggataaaaaa ataacggcaa tcagcaaacc gatcaatccc ttcggcaaat gatcaatcac
     2461 aaaacgaagg aaaatgtagt tggtatcatt gctgtcccct ttcacctctt tgtcatccag
     2521 ccatcttttg atcgttacgc ggagccgggc ctcctctctt tgggcatttt gcataaccag
     2581 gccctcctgc attcggttta ccacgggcgt tccaccggca gggttcatct gttccacttt
     2641 ctgtttttgc aggcggtcgt attccttcag cacgatctgc agagaatcgg catacttggt
     2701 ttttgaaagc aattcgattt gcgtctggtt aaagaacagg ggttctctgg caaactggta
     2761 gtatgcgaaa accagggtcc ccaaaagcaa aatgccaaac tgcatcggca ctttcaccaa
     2821 tccgttcagg agcagtccga gcttgctttc gcgggtggac cgggccgtca gataacgccc
     2881 gacctggctc tgatcggtgc cgaaataaga aagggcaaga aaaaacccgc cgatcattcc
     2941 gctccacata ttgtagcggt cattccagct gaatcctttt tccccctgcc ccgaggtgat
//
LOCUS       DASYCU010000375         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4286997, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000375 DASYCU010000000
VERSION     DASYCU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4286997"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VGM24_07560"
     CDS             complement(<1..584)
                     /locus_tag="VGM24_07560"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155267.1"
                     /translation="MVIKKPLLILLFAGLFNQGPAQGLKVNYSDPHIHYMGRIAFTDS
                     SAILSWTATSLKINFSGTGVKALLNDEHGDNYYNIIVDGKVIRILHPDSSKQLYTLAS
                     GLPPGNHLLELYKRTEWAMGKTGFYYFLLDKNAAVLPPPQTQKRKLEVFGNSITCGYA
                     VEDSTGQDRGTSPYENGYLSYAAILARHYDAEYF"
     gene            769..1083
                     /gene="rhaM"
                     /locus_tag="VGM24_07565"
     CDS             769..1083
                     /gene="rhaM"
                     /locus_tag="VGM24_07565"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY4155268.1"
                     /translation="MHKIAFKMKLKPGLRDEYKRRHDAIWPELRELLKESGISDYAIF
                     LDGETNTLFAVQSNPGKNSSQDLSGNPVIKKWWKYMADIMETNKDHSPVSVALDQVFY
                     ME"
     gene            complement(1071..2261)
                     /gene="nagA"
                     /locus_tag="VGM24_07570"
     CDS             complement(1071..2261)
                     /gene="nagA"
                     /locus_tag="VGM24_07570"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY4155269.1"
                     /translation="MNSQRIKIINGQIITPERIIPDGSLLVCDGLIRELSETPIQAPA
                     DLILDARGKFVSPGFIDIHVHGGGGADFMDGTVDAFLKIADTHAHYGTTAMLPTLLTA
                     GKEELLHCLDIYKQALNRNERGAGFMGLHIEGPYFDMEQRGAQDPRYIRNPDEKEYVE
                     IVEYAGGSIARWSAAPELPGALAFGRYMKSKGILPAIAHTNAVYEDVLKALENGYTLA
                     THFYSGMSGVMRRKAFRFAGVIESVYLLDEIDAEIIADGVHLPLPLLKLICKVKGTDR
                     IALITDAMRAAGMPPGESILGSKQNGIKVIVEDGVAKLPDRSSFAGSVATADRLVRTM
                     IRLAGVSLAEAVKMMTGVPARIMHFDQQKGSIAAGKDADLVIFDEDINIQTTMIKGRV
                     VHSI"
     gene            complement(2288..>2737)
                     /locus_tag="VGM24_07575"
     CDS             complement(2288..>2737)
                     /locus_tag="VGM24_07575"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY4155270.1"
                     /translation="NPEKECARLGSRIRENPIDLALVGIGENGHLAFNDPPADFETDK
                     PFIIVRLDEDCRRQQMNEGWFDSLSSVPEKAISMSIKQILSSGHIICSVPDQRKAAAV
                     RDCVTEEISNRHPASILRNHPNCFLYLDHGSASLVPELHLTDGAARS"
BASE COUNT          684 a          700 c          668 g          685 t
ORIGIN      
        1 caaaaatatt cagcatcata atgcctggct aaaatagcgg cataactgag atatccattt
       61 tcgtaaggcg aagttcccct gtcctggccc gtgctgtctt ccaccgcata cccgcaggta
      121 atcgaattgc caaatacttc cagcttcctt ttctgggttt gcggtggagg taggacagcg
      181 gcattcttat ccagtaaaaa ataatagaat ccggttttgc ccatggccca ttcggtccgt
      241 ttatataatt ccagcagatg atttcccggg ggcagaccgg aggccagggt gtaaagctgt
      301 ttgctgctgt ccggatgaag gatccggatc actttgccat ccacgataat attataatag
      361 ttatccccgt gttcgtcgtt taaaagcgct ttcacgccgg tgccggagaa gtttattttg
      421 agcgaggtgg ctgtccagga aagtatggcg gaactatcgg tgaaagctat gcgtcccata
      481 taatggatat gcggatcgga ataattcact ttaagaccct gcgccggacc ctgattaaac
      541 aggccggcaa aaagaaggat gaggaggggt tttttgatta ccatatatta aaatttgcag
      601 gctaaggacc aggcaacagg gcagggaatc ataaaaccac cattagatcg attttttcca
      661 atctgcatca gataacgaaa taatatggga cttttaccgc gtaaatctat ttaaaaggac
      721 gcaaatcatc cgggatctgc ggaatttatt taataatatc ttccacctat gcataagatc
      781 gctttcaaaa tgaaactcaa acccggctta cgggatgaat ataagcgcag gcatgatgcc
      841 atctggccgg aattaaggga attgctgaag gaatcgggaa taagtgatta tgctattttt
      901 ctcgacgggg aaaccaacac cctgtttgcg gttcaaagca atcccggaaa aaattcctca
      961 caggacctgt ccgggaatcc ggtgattaag aaatggtgga aatacatggc cgatatcatg
     1021 gaaacgaaca aagatcattc cccggtctcc gtagctctcg accaggtatt ttatatggaa
     1081 tgaacaaccc ggccctttat catcgtggtc tgtatattga tatcctcatc aaaaataacc
     1141 aggtcagcgt ctttgcccgc tgcgatcgat cccttctgct gatcaaaatg cataatgcgt
     1201 gccggcaccc ccgtcatcat cttaaccgct tccgcaagcg aaaccccggc gagccggatc
     1261 atggttctga ccagcctgtc ggcagtggcc acactgcccg caaaagaact gcggtccggt
     1321 aatttggcga cgccgtcttc cacgatcact ttgatgccat tttgttttga gcccaggata
     1381 ctttctcccg gaggcatgcc tgctgcccgc atggcatcgg tgatcagggc aatacgatcg
     1441 gtgcctttta ccttgcagat cagtttcagc agcggcaaag ggaggtgaac gccgtctgca
     1501 atgatctcgg catctatttc atccagcagg taaacagact caattacccc ggcaaagcgg
     1561 aaagccttac ggcgcatgac cccggacatc cccgaataga aatgcgtcgc cagggtatat
     1621 ccgttttcca atgccttcag cacgtcttca tacacggcat tggtatgcgc aatggccggc
     1681 aagatccctt ttgatttcat ataacggcca aaagccagcg ctccgggcag ttcgggcgcc
     1741 gcgctccatc tcgcaataga acctcccgcg tattctacaa tttccacata ctctttttca
     1801 tccggattcc ggatataccg cggatcctgg gctcctcttt gttccatgtc gaaatagggc
     1861 ccttcgatat gcaggcccat gaacccggca ccccgttcgt ttctgttcaa tgcctgctta
     1921 taaatatcca ggcagtgcaa taactcttct ttacccgcgg taagcagggt aggcagcatg
     1981 gcggtggtgc cgtagtgggc atgggtatcg gcaattttta aaaacgcatc taccgttccg
     2041 tccataaaat cggctccgcc gccgccgtga acatggatat ctataaagcc gggggaaaca
     2101 aattttcccc gggcatccag aatgagatct gccggagcct gaataggagt ctcgctcagc
     2161 tcccggatca gcccgtcgca aaccaataag ctgccgtccg gaattatccg ctccggagtg
     2221 atgatctgtc cgttgatgat ttttatacgc tggctattca tgaatctggg gtctgtaaaa
     2281 agaattttca ggaacgggcg gctccgtctg ttaaatgcag ctccggaacc agcgaagcag
     2341 acccgtggtc caggtataga aaacagtttg ggtgatttcg cagaatgctg gcgggatggc
     2401 gatttgatat ttcttccgtc acacaatccc ttaccgccgc agccttccgc tggtcgggta
     2461 cggagcagat gatgtgtccg gaggagagga tctgttttat ggacatgctg atggccttct
     2521 ccggaacaga tgatagggaa tcaaaccagc cttcattcat ttgttgccgg cggcagtcct
     2581 catccagacg aacaatgatg aagggcttgt ccgtttcaaa atcggccggc ggatcattaa
     2641 aggccaggtg gccgttttca ccaatcccga ccagggccag gtcgatcggg ttttccctga
     2701 tccggcttcc caaacgggca cattcttttt ccggatt
//
LOCUS       DASYCU010000376         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3417510, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000376 DASYCU010000000
VERSION     DASYCU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3417510"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VGM24_07580"
     CDS             complement(<1..223)
                     /locus_tag="VGM24_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4155271.1"
                     /translation="MNTRPIIEIKNLTKRFKRSTEDAVKDISFTVNRNEIFGLLGPNG
                     AGKTTTLSILCGLFPPTRGTVVIDGKNIRQ"
     gene            complement(270..1763)
                     /gene="crtD"
                     /locus_tag="VGM24_07585"
     CDS             complement(270..1763)
                     /gene="crtD"
                     /locus_tag="VGM24_07585"
                     /EC_number="1.3.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF042421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-hydroxycarotenoid 3,4-desaturase CrtD"
                     /protein_id="HEY4155272.1"
                     /translation="MDIAASGRSGHHKSCAVIGAGISGIAAAIRLRNKGWEVTVYEAN
                     AFPGGKLSSESIRGYRFDMVPSVFTMPEYVDELFLLSGKNPRDFFNYTPLDPVYRYFF
                     EDGSVLQARHGREQFAAVMAANSTDAKEDIIKYLKRSETIFSLTEDVFLKNSLHKLKS
                     FFTWPTLRGILNFGKIGAFDTMNAANSKAFTDSRLIQIFNRYATYNGSSPYLSPATLN
                     VIAHVEIVRGAYYPEGGMYSITKSLVQLAQDIGVQFRFSTPVREILTEKKKITGIRTD
                     AAKVAYEVVVSNMDIYNGYRQLLPDVKKPARILDQPKSSSGIIFYWGIKKEFSQLGLH
                     NILFSSHYAEEFDTIFSKKSIHPDPTVYLNITSKHTTGDAPPGCENWFTFINVPNNCG
                     QDWDELIGDARANIIKKINRLLKTDIEPLIECELVYDPRVIETRTSSAFGAIYGNSSN
                     NKYAAFLRHPNFSRQIRNLYFCGGSVHPGPSIPLCLLSAKITAGLVK"
     gene            complement(1766..2602)
                     /locus_tag="VGM24_07590"
     CDS             complement(1766..2602)
                     /locus_tag="VGM24_07590"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY4155273.1"
                     /translation="MITLFHELCEQCSKSTTRKYSTSFCSAIKLLHPELRTPIYNIYG
                     FVRFADEIVDTFHEYNRAELLLEFKRETYKAIERNISMNPILHSFQQTVKRHSIPMEL
                     IESFFKSMEMDLGGTLYNCSVYKEYIYGSAEVVGLMCLYVFCEGDAGMYKKLKPGAQA
                     LGAAFQKVNFLRDVKTDYEELHRTYFPEVDFQNFTPVKKKEIEAEIEKDFRDAYFGII
                     NLPAKAKFGVYVAYKYYLSLFHKIRRTAPSNILENRIRIPNYGKMYIVAKAGIRSQLN
                     IL"
     gene            complement(2651..4162)
                     /gene="crtI"
                     /locus_tag="VGM24_07595"
     CDS             complement(2651..4162)
                     /gene="crtI"
                     /locus_tag="VGM24_07595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEY4155274.1"
                     /translation="MKKAVIIGSGFAGLSAACFLAKAGLDTLVLEKQAIPGGRARKLE
                     ADGFVFDMGPSWYWMPEVFERFFGCFGKKVSDYYHLERLDPSYRVYWPEREMDIPADY
                     EALRNLFEREEAGSAVGLDRFMKEAEYKYKVGMNDLVYKPGLSLKEFMNPDLLKGIFK
                     LDVFTSMQSHIGKFFKNPCLRQLMEFPILFLGAMPRDIPALYSLMNYADVKLGTWYPR
                     GGMYRVVEGMYSLAKELGVKFLFNMNVREISTGNGMAKGVVAKSPSSEQHFFEADVVM
                     GAADYHYLEMNTLPAESRTHSEKYWDSRVMAPSCLLFYVGLNKKLKGIRHHSLFLDTD
                     FGEHGREIYSTRKWPADPLFYVCTPSVTDHSVAPPGKENLFFLIPVAAGLQGDTEVLR
                     EAYFQKILARFEARTGQTIREAIIHKRSYSVSDFLNDYNAFKGNAYGLANTLRQTAFL
                     KPSCRSNKIKNLFYTGQLTVPGPGVPPSLISGEIGAKEAIKYLHTFSHPVKTD"
BASE COUNT         1144 a         1113 c         1065 g         1265 t
ORIGIN      
        1 cctgccgtat attttttcca tcaatgacaa cggtgccgcg ggttggggga aataacccgc
       61 aaagaatgga gagggtagtg gtcttgccag cgccattggg gccaagcaaa ccaaatattt
      121 cattccggtt aacggtaaaa gagatatcct ttaccgcatc ttctgtactc cgtttaaacc
      181 gcttcgtcag attttttatt tctatgatgg gcctggtatt catttttcag ttataaaagt
      241 aaaacaactc gcattgcggc cggggtaatt tatttcacca ggccggcggt tattttggcc
      301 gacagcaaac acagtggaat gctggggccc gggtgaacac ttcccccaca gaaatacaga
      361 ttccggatct gtctggaaaa atttggatga cgcaaaaaag cagcatattt attattggaa
      421 ctgttgccat agatggcgcc gaatgcgctt gacgtacggg tttcaatcac gcgcggatca
      481 taaacaagtt cgcattcgat aaggggttcg atatctgttt tcagcaggcg gtttatcttt
      541 ttgatgatat tggccctggc atccccgatc agctcgtccc agtcctgacc gcaattgttc
      601 ggcacgttga taaaggtgaa ccagttttcg caacccgggg gggcgtcgcc ggttgtatgt
      661 ttactggtta tgtttaaata aacggtgggg tcggggtgaa tcgatttttt ggaaaaaatg
      721 gtatcgaact cttctgcata atgactgctg aataagatat tatgcaggcc gagctggctg
      781 aattcctttt tgatgcccca gtagaatata atgccggaac ttgatttggg ctggtccagg
      841 atcctggcgg gctttttaac atccggcaac agctgccggt atccgttata tatgtccata
      901 ttgctgacca ctacttcgta tgcgaccttt gctgcatccg tacggatacc cgttattttc
      961 tttttttctg tcagtatctc ccggaccggg gtggaaaatc gaaactgtac gccaatatcc
     1021 tgagcgagtt gcaccagaga ttttgtaatg ctgtacatgc ccccttccgg ataataggcg
     1081 cccctaacga tctctacatg ggcaatcacg tttaatgtgg ccggggacag gtaggggctg
     1141 gatccgttgt acgtggcata gcggttaaaa atctgtatca gcctggaatc ggtgaatgcc
     1201 ttgctgttgg ctgcattcat tgtatcaaaa gctccgatct tgccgaaatt caggatccct
     1261 ctcagtgtcg gccaggtgaa aaagctcttt agtttgtgca acgaattctt taagaaaacg
     1321 tcttccgtaa ggctgaaaat tgtttcggat cttttcaggt attttatgat atcctccttc
     1381 gcatccgtgc tgttggcagc cataacagcg gcaaattgtt ccctgccatg ccgcgcctgc
     1441 aacacgcttc cgtcttcaaa gaagtagcgg tacaccgggt cgagcggggt atagttaaag
     1501 aaatcgcgcg gatttttccc cgacagcagg aataactcat ccacgtattc gggcatggtg
     1561 aatacggaag ggaccatatc aaagcgatag ccgcggatac tttcggacga taactttccg
     1621 ccggggaaag cattggcttc atataccgtt acctcccagc ctttgttgcg caaacggatc
     1681 gcggctgcta tgccggaaat tcctgcgcca atcaccgcgc aggatttgtg atgccctgac
     1741 cttccggatg cagcgatatc catctttaca ggatatttaa ctggcttcgg atgcctgcct
     1801 ttgccacaat atacatcttg ccgtaattgg ggatgcgtat gcgattttcg agaatatttg
     1861 agggagcggt tcttcttatt ttatgaaaca gggaaagata atatttatag gccacgtaaa
     1921 cgccaaactt tgccttggcg ggcaggttga ttatcccaaa gtaggcatcc ctgaaatcct
     1981 tttctatttc ggcttcgatt tcttttttct ttaccggtgt gaaattctgg aaatcaactt
     2041 cggggaaata ggtccggtgg agttcttcgt agtcggtttt aacatcccgc aggaaattaa
     2101 ctttctggaa agcggcgccc agcgcctgtg ccccgggttt aagcttttta tacatgccgg
     2161 catcgccttc gcaaaaaaca tacaggcaca tgagtcccac cacttcggcg ctgccataaa
     2221 tatattcttt gtatacgctg caattgtaaa gtgttccacc caggtccatt tccatgcttt
     2281 taaaaaaaga ttcgattaac tccattggaa tggaatggcg tttgacggtt tgctgaaagc
     2341 tatgcagaat gggattcatg ctgatgtttc tctcaatcgc tttatacgtc tcgcgcttga
     2401 attcaagcag cagttcggcc ctgttgtatt catgaaaagt atctacaatt tcatcggcaa
     2461 agcgtacaaa cccgtagata ttatagatgg gggttcgtaa ttccggatgt aatagtttga
     2521 tggcgctgca aaaggaagtg ctgtatttcc gggtcgtgct tttactgcac tgttcgcaga
     2581 gttcatggaa tagggttatc atgcctgata aggtttatgg gttattgata aaaacagcgt
     2641 atttgttctt tcaatctgtt ttaacggggt gcgaaaatgt atgcagatat ttaatcgctt
     2701 cttttgcccc tatttcacca cttatcaggc tgggaggcac cccaggaccc ggcacggtca
     2761 attgaccggt gtaaaatagg ttttttattt tattacttct gcaggaaggt tttaaaaaag
     2821 ccgtttgtct taatgtattg gcgaggccat acgcattgcc tttgaatgcg ttgtaatcat
     2881 tcagaaaatc gcttacggaa taggatcttt tatggataat ggcttcccgg attgtctggc
     2941 ccgttctggc ctcaaaccgg gcgagtattt tttgaaaata agcctcccgt aatacttccg
     3001 tatctccctg taaaccggca gctaccggga tcaaaaagaa caaattctct tttcccggag
     3061 gcgccaccga atggtccgtt acggaaggcg tacatacgta aaataagggg tctgccggcc
     3121 atttccgggt tgaatagatt tctctgccat gttccccgaa atcggtatcc aggaatagag
     3181 aatgatgccg gatacccttt aatttcttat ttaatcctac gtaaaacagg aggcaactcg
     3241 gcgccatgac cctggaatcc caatactttt cgctatgagt tctgctttcc gcaggcaggg
     3301 tattcatttc caggtaatgg tagtccgccg cgcccatcac cacatccgcc tcaaaaaaat
     3361 gctgctccga cgaggggctt tttgccacaa ccccctttgc catcccgttc cccgtgctga
     3421 tctcgcgtac attcatgtta aaaaggaatt tgacacccag ttctttagcc agcgaataca
     3481 ttccttcaac cacccggtac atgccccccc ggggatacca ggttcccaat tttacatccg
     3541 cataattcat caggctgtat aaggcgggta tatcccgggg catggctccc aaaaacagga
     3601 tggggaattc catcagttgg cgcaaacagg ggttcttaaa gaactttccg atatgggatt
     3661 gcatggaagt gaatacatcc agcttaaaga tccctttgag gagatccggg ttcatgaatt
     3721 ccttaagcga caggcccggt ttataaacga ggtcgttcat tcccactttg tacttatact
     3781 ccgcctcctt catgaaccga tccagaccta cggcggatcc cgcttcctcc ctttcaaaaa
     3841 gatttctcaa ggcttcataa tccgccggaa tgtccatttc ccgctccggc cagtagaccc
     3901 ggtaagaggg atcaagccgt tcgaggtggt agtagtcgga aacctttttc ccaaaacagc
     3961 caaagaaccg ctcaaaaacc tccggcatcc agtaccagct gggccccata tcgaaaacaa
     4021 atccatcagc ctccagcttc ctggcccggc caccgggtat ggcctgtttt tccaatacca
     4081 gggtatccaa acccgctttc gccaaaaaac aggccgccga taaaccggcg aatccgcttc
     4141 ctataattac cgcttttttc acaggatgaa aatacgaata ttgcgtatcg gccaatgtaa
     4201 aaacagaaag ccgtgtatct cttatgttct gagacaaagc tcggggtata aataaggaat
     4261 ttgcggaata cccgaccaac gggcttgtaa aatcgtcgaa catctttacc aatccgcaag
     4321 tcagcctgtc tccgcttttg gacgatgaaa gaaagatgtt tgccgggcaa tcgggttatt
     4381 aagtcgtaat gactcgtccg cgaaaggatc atcgaagcca gcgtccgcca acctcaggat
     4441 cctattctgc cctcagactc ttcaccggat tggcaaccgc tgctttgatc aactgggaac
     4501 ttatcgtaac gagtgcgatc agtaaacaga tagcgccgac ctctacaaac atccaccagc
     4561 taagtcggat tcggccggcc tgcgtat
//
LOCUS       DASYCU010000377         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3995842, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000377 DASYCU010000000
VERSION     DASYCU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3995842"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VGM24_07600"
     CDS             complement(<1..985)
                     /locus_tag="VGM24_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155275.1"
                     /translation="MKKLLLILTLNISLLFSPPPKAGTTDPNGRYVSLSPFAGFFLNP
                     DTYGFIFPAIEPGQLLTSQSQRQSRPLFILAGSAIGYAITGLTWPFHNRLLRLYGAFW
                     RGTYPADRILLIGNFYLGFLLLNVLVLWLSLFLFERIFFSGIRRSRDAEYSLYFLMVF
                     IVSNPVTKAFFWTVHQQMFSFLTPIFCIYVLLWFNRRPDALSGRQMIMLFLGGGALLL
                     VYGNFLLLLPVMLFGFFQQFRKFPGQESRLLLKASGLFLVFFLPTILWIGILKWTGVS
                     YYSFELQGYHEIIWIPETLHYSVGSFLKQLGQNTLEFLGTLRQFGILMLFSV"
     gene            complement(994..1581)
                     /locus_tag="VGM24_07605"
     CDS             complement(994..1581)
                     /locus_tag="VGM24_07605"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY4155276.1"
                     /translation="MFTGIIEAQGLLQAITRTGANKTFRISSPLSAGLKMDQSLSHDG
                     VCLTVESVSGDSHEVTAIAETLAKSRLNQWQPGDLINLEQCVQLNGRLDGHIVQGHVD
                     TVAVCLSREDCDGSLEYRFEFPESFGHLVIEKGSVAINGISLTCFEVTENRFSVAIIP
                     YTLEHTNMKSVIEGSVVNIEFDMIGKYVSRLASNF"
     gene            1679..1876
                     /gene="rpsU"
                     /locus_tag="VGM24_07610"
     CDS             1679..1876
                     /gene="rpsU"
                     /locus_tag="VGM24_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY4155277.1"
                     /translation="MLIIDSKDCENIDKALKKYKKKFEKAKVLIQLRNRQSFTKPSIK
                     RRGEVLKAVYKQQLASGKFEL"
     gene            1949..2848
                     /locus_tag="VGM24_07615"
     CDS             1949..2848
                     /locus_tag="VGM24_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY4155278.1"
                     /translation="MPFPLDASVRSFIDFLKFEKRYSQHTVKAYSDDLDQFFCFLDSL
                     FGKQDLRQVSTPIIRSWLAGLKEKKISSRSIKRKISSLKSFFKYLVRTGALEKTPMSG
                     IISPKSGKRLPVYVAEKDMELLTRHLEFPDNWQGKTDRLLIGIFYQTGMRLSELVNLS
                     TDQVDLEAAMVRVLGKGNKERMIPLSAELGKEIRLYIREKNESGRNIEKNLLVGPQGK
                     KLYTKYAYLAVRKYLSQVTTLSKKSPHVLRHSFATHLMNHGADLNSVKELLGHASLAA
                     TQLYTHNSIEKLREVHRKTHPRG"
BASE COUNT          882 a          713 c          716 g          701 t
ORIGIN      
        1 ttacagaaaa aagcatcaat ataccgaact gccgcagggt acccagaaat tccagggtat
       61 tttgtccaag ttgttttaaa aatgaaccca ccgagtaatg cagggtttcc ggaatccata
      121 tgatttcatg gtatccctgc agctcgaaac tataatagga aacccccgtc cattttaata
      181 ttccgatcca aaggatcgtc ggaagaaaga aaaccaggaa caatccggaa gctttcagta
      241 atagcctgga ctcctgaccg ggaaattttc tgaattgctg aaaaaacccg aatagcataa
      301 cgggcagcag caaaagaaaa ttaccgtata ccagcaggag tgctcccccg cccaaaaaaa
      361 gcataatcat ttgccgaccg gaaagggcgt ccggcctgcg gttaaaccac agcaggacat
      421 aaatacagaa aatgggtgtc agaaaggaaa acatctgttg atggaccgtc cagaaaaagg
      481 ccttggtcac cggattggat acgataaaga ccatcaggaa atacagggaa tattccgcat
      541 cccggctgcg gcggatcccg ctaaagaaaa ttctttcaaa caaaaaaagg gatagccaca
      601 agaccagcac attcagcagc aggaatccta aataaaaatt tccaatgaga agaattctat
      661 cggcgggata ggttccccgc cagaatgctc cgtacaaccg gaggagccgg ttgtggaatg
      721 gccaggttaa cccggtaatg gcatatccta tggcggatcc ggccagaatg aacagcggcc
      781 gggactggcg ctgggactga ctggtcagca actggcccgg ttcaatggcc ggaaatatga
      841 agccgtaggt atccggattt aaaaagaagc ccgcaaaagg acttaaagaa acataccgcc
      901 cgttcggatc cgttgtgccc gctttggggg gaggagaaaa taagagggag atgttcaggg
      961 tcaggatcag caatagtttt ttcataccct tttttagaag ttggacgcca gcctggaaac
     1021 atactttccg atcatgtcaa actctatatt cacgacagat ccttcaatga ccgatttcat
     1081 attggtatgt tccaacgtat atggaataat ggccacgcta aacctgtttt cggttacctc
     1141 gaagcaggtc aggctgattc catttattgc gacagatccc ttttcaatga cgaggtggcc
     1201 gaaggattcc ggaaactcaa accggtattc caggctcccg tcgcagtctt cgcggctaag
     1261 gcaaacggcc acggtgtcca cgtgcccctg cacgatatgt ccatccaggc ggccattcag
     1321 ctgtacacac tgctccagat tgatcaggtc gccgggctgc cattggttca gccggctttt
     1381 ggcgagggtc tccgcgatgg ccgtcacttc atgcgaatcc ccggaaaccg attccacggt
     1441 caggcagacc ccgtcgtggg aaagactctg atccatcttt aatccggccg acagcgggga
     1501 agaaatccgg aacgttttgt tggccccggt ccgggttatg gcctgcagca ggccctgggc
     1561 ctcaatgata ccggtaaaca tgctgtaaaa atcgcatttt ccgggctttt tattacttta
     1621 ttttgaaaaa ctccctatct ttgcaggccg taattcaatt ttaaaagcgg aaattattat
     1681 gctcattatt gattcaaaag attgcgagaa cattgacaag gccctgaaga agtacaagaa
     1741 aaagtttgaa aaagccaagg tactgattca gttgaggaac cgccagtctt ttaccaaacc
     1801 ttccattaaa agacgggggg aagtacttaa ggcagtgtac aaacagcagc tcgccagcgg
     1861 taagttcgaa ctataacacc ggtttcccac agcattatat atggccatct ttccggatgg
     1921 cctttttatt ttatatttgg atgaatcaat gccctttcct ttggatgcat ccgtccggtc
     1981 atttattgat ttcctgaaat ttgaaaaaag gtattcccaa cataccgtca aagcatattc
     2041 agacgatctg gatcaattct tttgtttcct ggattccctt tttggcaaac aggatctcag
     2101 gcaggtttcg actcccatta tccgttcctg gctggccggc ctgaaagaaa agaagatcag
     2161 ctccaggtcc attaaacgca agatctcctc attgaaatca tttttcaaat acctggtgcg
     2221 aacgggcgcc ctggaaaaaa caccgatgag cgggattatc agccccaaat ccggcaaaag
     2281 acttccggtg tatgtggcgg aaaaagacat ggagttgctc acccgccacc tggagttccc
     2341 ggataactgg cagggaaaaa cggaccggct gttaatcggc atcttctatc aaaccgggat
     2401 gcggctgagt gaactggtga acctcagtac ggatcaggtg gacctggaag cagctatggt
     2461 ccgggtgctc ggaaaaggga ataaggaaag aatgatcccg ctgagcgctg aattgggcaa
     2521 agaaatccgg ctttatatcc gggaaaagaa tgaatccgga agaaatattg aaaaaaatct
     2581 tttggtcggt ccccagggca agaaattata tacaaaatat gcttatctgg ccgtcagaaa
     2641 atatttatcc caggtgacca cgctcagtaa aaaaagcccg catgtgcttc gccacagctt
     2701 cgccacccat ttaatgaatc atggagccga cctgaattcg gttaaggaac tgctcgggca
     2761 cgccagtctg gccgcaaccc agttatatac ccataacagc attgaaaagc tccgggaagt
     2821 gcatcggaaa acccacccgc gcggataaat tcaccggttg aattggctac aatttatggg
     2881 gcaaatattt ggaaaaatta agctaaggcc ttaatttcaa tccaccgcgg gacagacctg
     2941 accggcgggg aatgagttct tttaaatatt gtttcatcgt ttaaaaagcg aagctatgaa
     3001 cgttaatatt ca
//
LOCUS       DASYCU010000378         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2981808, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000378 DASYCU010000000
VERSION     DASYCU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2981808"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VGM24_07620"
     CDS             complement(<1..491)
                     /locus_tag="VGM24_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155279.1"
                     /translation="MKKTNCIPAVLMALGFVALSATGYSQSRNSGEVSPVKTGSAILN
                     LGIGVGADYKNSYGTGFGFKAAAEFGIWQAGPGVITLGPEIGGSFSNGGYYDNYRSNT
                     FVIAGRAAWHYGWQVPGLDTYGGFSAGIGFHHYGYRNGTDYSYNEVIPAFGGFVGASY
                     FLSP"
     gene            881..1273
                     /locus_tag="VGM24_07625"
     CDS             881..1273
                     /locus_tag="VGM24_07625"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY4155280.1"
                     /translation="MSSLEMDNLTGANQGAIFPNLPLKYLTASSVIGDKVRNNANEHL
                     GEIKDIMLDITTGKIDYFVIEFGGFLGIGIKYFAIPFRLIRVDAEKKMFVFNQSKEAL
                     EKAPGFDIEHWPDTNLHLDQVYSYWSFF"
     gene            complement(1289..1471)
                     /locus_tag="VGM24_07630"
     CDS             complement(1289..1471)
                     /locus_tag="VGM24_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEY4155281.1"
                     /translation="MNKTLITLLIGIGIGILLAPDKGSETWKKLKDGLNDFKDKLADE
                     ADELGEQAENIVREER"
     gene            complement(1590..2312)
                     /locus_tag="VGM24_07635"
     CDS             complement(1590..2312)
                     /locus_tag="VGM24_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY4155282.1"
                     /translation="MDHLMDFHPDCFLCHFALDAWQPAIEANASCLHFKRGEHLFKEG
                     EPVKGFYFIHSGKVKIHKCWGDSKDLIIKFAGKMNILGHRGMLGNQAYPVSATAIEDC
                     AVCFITTEFFNTTLLVNHELAYQMVLFYADELQQAELGMRNMVHMDVKSRIADSLLKL
                     QESFGSDPEGFIDCKLTRQDVASYAGTTYETLFKTLQELTEAKIIGTCGKSICIRNKN
                     KLRNLIKLPQNKIPVATGQTIS"
     gene            2438..>3140
                     /locus_tag="VGM24_07640"
     CDS             2438..>3140
                     /locus_tag="VGM24_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY4155283.1"
                     /translation="MLRDNTPKEEGIPIAPALALEDEDSSAKPVVSTRLFFISVQVVF
                     NAIMIGLMAKVMVALINLITNISFYGKFSLAEASPAGNHLGLWVIIVPVIGGLLVGIM
                     AKVGSKAIRGHGIPEAMEQVLTNKSKIKPVVTLLKPISAAFSIGMGGPFGAEGPIIST
                     GGAFGSFTGQLMHITANERKVMLTAGATAGMAAIFGTPISSVLLAVELLLFEFSPRSM
                     IPVALACATGAAMHLL"
BASE COUNT          852 a          725 c          707 g          856 t
ORIGIN      
        1 ggcgataaaa aataggaagc gccgacaaag cctccgaatg cgggaatgac ttcattataa
       61 ctatagtcgg tcccattgcg gtatccatag tgatgaaatc ctattccggc agaaaaaccg
      121 ccataggtat ccagtcccgg aacctgccag ccgtaatgcc aggctgcccg gccggcaatc
      181 acgaaggtgt tggagcggta attatcgtaa tagcctccat ttgaaaagga gccgccaatt
      241 tccggcccta aggtaatgac ccccggaccc gcctgccata ttccgaattc ggcagcggct
      301 ttaaatccaa aacccgttcc gtaactattt ttgtagtctg cgccgacacc aatgccaaga
      361 ttgagaatgg cactacccgt ttttaccgga gacacttctc cactgtttcg cgactgcgaa
      421 taaccggtag ccgaaagagc aacaaaacca agcgccatta atactgctgg gatacaattc
      481 gtttttttca taactgcaaa gataattacc tgttatccta ataaaaaagg gccaggataa
      541 aaatccagcc ccgtttaaaa tccaatatcc tatgaaaaac caattagact taaacaagcc
      601 acataccgga aatgaatgat tgttatataa taaaataaat aatatgtaat aactgtaaag
      661 taagcatttt aacaaactat tttgggataa atttgttttg aaccggatag ttttgaaaaa
      721 tcagaattga ccaatttttc tcctttgtgg aaatattatc ccatttagag caattggcgg
      781 gaatgaaatt tgcttgtgaa aaccggcagt aagggaaaac aatacggtat gtgcaaaatt
      841 tagaggtata ccgccgcctt tttaattaaa aactattttt atgagttcat tggaaatgga
      901 taatctgacc ggcgctaacc agggagctat ttttccaaat ctcccgctga aatatctgac
      961 ggcttcatct gttatcggcg acaaggtcag gaataatgcg aacgagcacc tcggggaaat
     1021 aaaagatatc atgctggata ttacgaccgg taaaattgat tatttcgtaa ttgaattcgg
     1081 cggttttctc ggaatcggaa tcaaatactt tgcaatcccg ttccggctga tccgggtgga
     1141 tgcggaaaaa aagatgttcg ttttcaatca gtcaaaggag gcgcttgaaa aagcacccgg
     1201 gttcgatata gaacactggc cggataccaa tctgcatctc gaccaggttt acagctactg
     1261 gagctttttt taataaaaca gccggctgct atctttcttc ccggacaata ttttctgcct
     1321 gctcacccag ttcatctgct tcatcagcca gcttgtcttt aaaatcgttc aggccgtctt
     1381 tcagtttttt ccatgtttcg gaacccttat ccggggcaag cagaataccg ataccgattc
     1441 cgataaggag ggtgatcaat gttttgttca taaatgttgt tttaagttga gtttcaaatt
     1501 tcaagccagc gaagaggaac ttccggctgc ccggcggcgc taccggaatt tcctatgcgt
     1561 ggtttgcgca aactccttcc ggtccccatt cagcttatgg tctgccctgt tgcgacaggg
     1621 attttatttt ggggaagttt gatcagattc cgcagtttat ttttatttct gatgcagatg
     1681 ctttttccac aggtcccgat aatttttgct tcggttagtt cctggagggt cttgaaaagg
     1741 gtttcgtagg tggtgccggc atacgaagcc acgtcctggc gggtgagttt gcaatcgata
     1801 aatccctccg gatccgatcc aaaactctcc tggagtttca gtaaactatc tgcaatccgg
     1861 ctttttacgt ccatgtgcac catattgcgc atgcccagtt cggcctgctg caattcatct
     1921 gcataaaaca gcaccatctg gtaagccagc tcgtggttta ccaataaggt cgtattgaaa
     1981 aactcggtgg tgataaagca aacggcgcaa tcttcaatcg ctgtggcgga aacggggtaa
     2041 gcctggtttc ccaacatgcc ccggtggccg agtatattca tttttcctgc gaacttgatg
     2101 atcaggtcct tggaatcccc ccagcattta tgaattttta ccttaccgga atggataaaa
     2161 taaaacccct ttaccggttc tccctcttta aataaatgct ctcccctttt aaaatgaagg
     2221 caggaggcgt ttgcttcaat ggcgggctgc caggcatcca gggcaaaatg gcaaagaaag
     2281 caatccggat ggaaatccat gaggtgatcc attaaaatga gtaaatttcg gttcctatga
     2341 aaacattgct aaaataatgt tttataataa aaattaaggt taaagctgag gaaatgtact
     2401 ggtaaggcaa tatttttgca aaaaatttta cttttccatg ctccgagata atacccctaa
     2461 ggaagaaggt attccgatcg cccccgccct cgcattagaa gatgaagatt cttccgcaaa
     2521 gccagttgtt tccacccggt tattttttat cagcgtccag gtggttttta atgccattat
     2581 gatcggcctt atggcaaagg taatggtggc cctgatcaac ctgataacca acatttcctt
     2641 ttacggaaaa ttttctttag cggaagcgag cccggcggga aatcacttag ggctttgggt
     2701 aattattgtg cccgtcatag gcggactgct ggtcggcatc atggcgaaag tcggatccaa
     2761 agcgatccgc ggacacggca tcccggaagc catggagcag gtgctgacca ataagagcaa
     2821 gataaaaccg gtggttactt tgctcaaacc catatcggct gccttttcga tcggtatggg
     2881 tggtcccttt ggcgcagaag ggccgatcat ttctaccggc ggtgcattcg gatcattcac
     2941 cggacaactc atgcacataa cggcgaatga acggaaagtg atgctgactg caggggccac
     3001 ggccggaatg gcggccatct tcggcacccc gatctcatcg gtgttgctgg cggttgaatt
     3061 attattattc gaattttctc cgcgctccat gatccccgtt gccctggcct gcgcaaccgg
     3121 tgccgccatg catctgctcc
//
LOCUS       DASYCU010000379         4372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3709340, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000379 DASYCU010000000
VERSION     DASYCU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3709340"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /gene="radC"
                     /locus_tag="VGM24_07645"
     CDS             <1..493
                     /gene="radC"
                     /locus_tag="VGM24_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY4155284.1"
                     /translation="RLSIHDLLKIKGIGRAKAVIILAALELGRRRHIMGALDKPVIRD
                     SHQVADYLRVTLQDNCREVFAVLFLNQANRVNHFEVVSQGGITGTVADPRIILKRALE
                     SNAVNVILCHNHPSGNLRPSKADEDLTYKIREAAKYFDIKVIDHIIVSDDGYFSFADS
                     GIL"
     gene            complement(551..859)
                     /locus_tag="VGM24_07650"
     CDS             complement(551..859)
                     /locus_tag="VGM24_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEY4155285.1"
                     /translation="MADPVNNQLNIEISEEVAEGEYANLAIITHSHAEFVIDFVNVMP
                     GTPKSKVKSRIIFTPQHAKRFMKALSENVNRFEASNGKIQDLEDVQIPMNFGGPTAQA
                     "
     gene            complement(973..1452)
                     /locus_tag="VGM24_07655"
     CDS             complement(973..1452)
                     /locus_tag="VGM24_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding protein"
                     /protein_id="HEY4155286.1"
                     /translation="MMKNITLLLLCIVIATVSCKKSTFDPGTTYAKKVANGWWVTALE
                     NGTDVGVGYFFLSTYNTSGQPDSLWVDDNEQFWEFKVKAKIDMSALTLTTDNAQNEYY
                     DSHVTIDNGKILLKAGHSTSGVITDSIYMEAHFDDDPDAATWTITGTARTGFIEDDH"
     gene            complement(1497..2144)
                     /locus_tag="VGM24_07660"
     CDS             complement(1497..2144)
                     /locus_tag="VGM24_07660"
                     /inference="COORDINATES: protein motif:HMM:NF027718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HEY4155287.1"
                     /translation="MKISFSSFVVLLILGGMISCKKDNFNYAKDTVGSSRITYFATFN
                     MAGDPYVSVVQGDTYTDAGATASQNGTDLPVTVTGEVDANTVGIYTLTYSATNSDGFP
                     ASVSRTIAVLPSAELPGVDISGSYYYISTGANNSTVTKLAPGFYSTTNCWSSATTIPC
                     LFICVDGQNIIMPNQPTGFGELYGTGTLSSTGALTYLVTIESQGIFGTARKWHLK"
     gene            complement(2163..3659)
                     /locus_tag="VGM24_07665"
     CDS             complement(2163..3659)
                     /locus_tag="VGM24_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEY4155288.1"
                     /translation="MNRLKIKISAPLLVFSFLILGTGCKHTLDINQNPNFPTLDQGTP
                     SLVLPPAVLATTGKIGADLAIVGGIWSQYFTQAALASQYKPVDSYNMPNTDFFVNDVY
                     DGLFTTGLKNYQYVIDKSRESADWNTFLIATVMKAYTTGIMVDMYDQIPYSEALGGAA
                     QLKPKFDDGYDIYTSLLSSLDSALNGDFSAGTNTNLGATDLVFGGDVGQWKLFANTLK
                     LKFYLRMINAHADVAQSGITAMMSAGTSFLASDAAVTNFTDAPSQDNPFFEWNQRTLN
                     TTANLKASATFVLWLEQANDPRIVSYFGNPTPNYVHQGDYSGSDPTYQTAATFVQSPT
                     DPVEFISGAESYFLQAEADVRYNSGANAKALYDQGVLTAFADLGLDGSSFIEPGGAYE
                     WGNESEDGVLLTPIQQIIRQKWASFPYGCHQIEGFFDKNRTGFPLTSAVYSTEPGYVP
                     GQFVVGKNSVLDPGLLPKRFVFPYDETSRNSNAPQTVVPISTPVWWAQ"
     gene            complement(3679..>4372)
                     /locus_tag="VGM24_07670"
     CDS             complement(3679..>4372)
                     /locus_tag="VGM24_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155289.1"
                     /translation="SPDGKIVVNPANGLPVVNTSPLDAYGLQNGYYGSGLPNYTMGFT
                     NNLRYKNFNFNFSFDFRYGGVMFSQTASLSEFVGNSKATIYNDRKPFIVPNSVNAVVD
                     GSGKTSYVENKTFIGATGNGQSDNYYEYWNNPANIGSLYQSTIFDRSFLKLRDINLSY
                     TLPAKWASKIGSSQASVGVYGRNFLLWTPKSNIYVDPEATNFGNDLIGNFGEFATGPL
                     SKSYGVILKLTF"
BASE COUNT         1133 a         1038 c         1046 g         1155 t
ORIGIN      
        1 caggctcagc attcacgatt tattgaaaat aaagggaata ggccgggcaa aagcggtgat
       61 catcctggcg gcactggagc tggggcggcg tcgtcatatc atgggagccc tggataaacc
      121 cgttatccgg gatagccatc aggtagctga ctacctgcgt gtaacgttac aggataactg
      181 ccgggaggtt tttgccgtat tatttctgaa ccaggctaac cgggtaaatc attttgaagt
      241 cgtcagccag ggcggtatca ccggaaccgt ggccgatccc cggatcatcc tgaaaagggc
      301 cctggaatcc aacgcggtca acgtgatcct ttgtcataac catccttccg gcaatctgag
      361 gcccagcaag gcggatgagg acctaaccta caagatccgg gaagcggcaa aatactttga
      421 cataaaagta atagaccaca tcatcgttag cgatgacgga tatttcagtt ttgcagattc
      481 gggcattttg tagtgaaccc gggatggttt tttcacatcc tgaaagcggg cgggccgctt
      541 ttacttagat tcaggcctgc gccgtgggac ctccgaaatt catcgggatc tgcacatctt
      601 ccaggtcctg aattttaccg ttcgaggctt caaaccggtt cacattctcc gagagtgctt
      661 tcatgaagcg cttggcatgc tggggcgtga aaataattct cgatttcacc ttgctcttgg
      721 gagttcccgg cataacattt acaaaatcga tcacaaactc agcatgactg tgcgtgatga
      781 tggccagatt agcatattcc ccttcagcca cttcttcact gatttcaata tttaactggt
      841 tattgactgg atctgccatt cggatgattt ttatttgtca aaagtacaat ttcatcgaac
      901 accaaagggc ctgtaaataa aaaaaggccc ggaggccttg tattttaatg catcgtacat
      961 tgcgtcacgc catcaatgat catcctcgat gaacccggtc cttgctgttc cggtgatggt
     1021 ccaggtggct gcatccggat catcatcaaa gtgcgcttcc atatagatgc tgtccgtgat
     1081 cacaccggaa gtggaatgac cggctttgag caggatcttt ccattatcaa tggtcacatg
     1141 gctgtcgtaa tattcgttct gtgcattatc tgtggtcaag gtaagcgcgg acatgtcaat
     1201 ctttgccttc accttgaact cccagaactg ttcattgtca tccacccaaa gggaatccgg
     1261 ctggcctgag gtattatagg tcgatagaaa aaaatatccg acacccacat ccgttccgtt
     1321 ttccaacgcg gtgacccacc agccattggc cactttcttg gcataggtgg tgccggggtc
     1381 aaaagtgctt tttttgcaag atacggttgc tattactata caaagcaaca gcagggtaat
     1441 attcttcatc ataaaaattt tatttgtttt aaatgcccgt ttcaatagcg aaataattat
     1501 ttgagatgcc atttccttgc cgtaccaaaa attccctgtg attcaatcgt aaccaggtag
     1561 gtcagtgctc cggttgaact cagggtgccc gtaccgtata attctccaaa gccggtaggc
     1621 tggttaggca taattatatt ttgcccgtca acgcaaataa acaggcaggg tatggtcgtt
     1681 gcactgctcc agcagttcgt ggtggaataa aatccgggag ccagtttggt gactgtggaa
     1741 ttattggcgc cggtactgat atagtaatag ctgccggata tatccacccc cggtaattcc
     1801 gcactcggca acacggcaat ggttctcgat acggaagcag gaaaaccgtc ggagttggtt
     1861 gcggaatagg tcagggtgta aatgccaacc gtattggcat ccacctcacc ggtgaccgtt
     1921 accggcagat ccgttccgtt ctgtgaggcg gtagccccgg cgtcggtata ggtatctccc
     1981 tgaacaaccg aaacataagg gtcgccggcc atattgaatg tggcaaaata cgtgatcctc
     2041 gaactgccta cggtatcttt cgcataattg aagttgtcct tcttacagga aatcattcca
     2101 cctaggatca gcaggacaac aaacgatgaa aaacttattt tcattttatt cttttttttt
     2161 gattattgag cccaccaaac gggggttgaa atgggtacaa cggtttgcgg tgcatttgaa
     2221 ttccgggaag tttcatcata agggaaaaca aaccgtttgg gaagcagccc cggatccaga
     2281 acggaatttt ttccaactac gaactgaccg ggaacataac cgggttcagt agagtatacc
     2341 gcgctggtga gcggaaaacc ggtcctgttc ttatcaaaga atccttcaat ctggtgacag
     2401 ccgtagggga aggaggccca tttctgccgg atgatctgct ggatgggcgt taacaacacg
     2461 ccgtcttccg attcgttccc ccattcgtag gctccccctg gttcaataaa ggatgaacca
     2521 tccagtccga gatccgcaaa agcagtcagc acaccctgat catataaggc ttttgcattt
     2581 gcgcctgaat tataacggac atctgcttcc gcctgcaaaa agtaagactc ggcgccggaa
     2641 ataaattcaa ccggatcggt agggctctga acaaaagtgg ctgccgtctg ataggtcgga
     2701 tcacttccgc tataatcgcc ctggtgcaca taatttggtg tggggttacc gaaataactg
     2761 acgatccgcg gatcattggc ctgttccagc cataatacaa atgtcgcgct ggctttcagg
     2821 ttggccgtcg tattgagtgt ccgttggttc cattcgaaaa atggattgtc ctggctcgga
     2881 gcatcggtga aattggtgac cgccgcatcg ctggcaagga aagaagttcc ggcagacatc
     2941 atcgcggtaa tgccggactg tgccacatct gcatgggcat tgatcatccg gagatagaat
     3001 ttcagtttaa gcgtattggc aaaaagcttc cactgaccta catctcctcc gaaaaccagg
     3061 tcggtggcgc caagattggt attagtcccc gcgctgaaat caccattcaa tgcggaatca
     3121 aggctgctga gcagggaggt ataaatatca tacccgtcgt caaattttgg cttcaactgg
     3181 gcagcgccgc ccagggcttc ggaatagggt atctgatcat acatgtccac cataatacct
     3241 gtcgtatacg ctttcatcac cgtagcaatc aggaaagtat tccagtctgc cgactctctc
     3301 gatttatcaa ttacgtattg atagttcttc aggcccgtgg taaaaagacc gtcatatacg
     3361 tcattgacaa aaaaatcagt gtttggcata ttgtacgagt ctaccggttt atactggctt
     3421 gccagggcag cctgtgtaaa atactgggac cagattccgc ctacgatggc cagatctgct
     3481 ccgatctttc cggtagtagc caataccgct ggtggtaaaa caagcgatgg cgttccctgg
     3541 tcaagggttg gaaagttagg gttctgatta atatccagcg tgtgtttaca tcctgttccc
     3601 aagatcagga atgaaaaaac cagaagcggg gcggatattt ttatttttaa tcggttcata
     3661 ataaattcgg tttttccttt agaaagttaa tttaagaatt actccatagc tttttgaaag
     3721 aggtccggtg gcgaactctc caaaattacc tatcaggtca tttccaaaat tcgtagcctc
     3781 cggatctaca tatatattgg acttcggtgt ccacaacagg aaattcctcc catacacgcc
     3841 aaccgacgcc tgtgaagaac caattttgga agcccatttg gccggcagcg tataggagag
     3901 gtttatatca cgcagtttca ggaaagaacg gtcgaaaatg gtgctctgat aaagcgaacc
     3961 aatatttgcc ggattgttcc agtactcgta ataattatcg ctctggccgt taccggtggc
     4021 gccgataaat gttttattct ccacatagct ggtcttaccg cttccgtcca cgaccgcgtt
     4081 gacggaatta ggaacgataa acggtttacg gtcattatag atggtggcct tgctgttccc
     4141 aacgaactca gaaagagatg ccgtctgact gaacatcacg cccccatatc tgaaatcaaa
     4201 ggagaaatta aaattgaagt tcttatatct caggttattg gtgaatccca tggtataatt
     4261 aggaagaccg cttccataat aaccgttttg caggccatag gcgtcaagtg gggaggtatt
     4321 gacaaccggc aacccgtttg ccgggttaac cacgatcttt ccatccggcg at
//
LOCUS       DASYCU010000380         9573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_956167, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000380 DASYCU010000000
VERSION     DASYCU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956167"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VGM24_07675"
     CDS             complement(<1..664)
                     /locus_tag="VGM24_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY4155290.1"
                     /translation="MNEDRLWLLVARHLAEEASVSEAEELQLLLKQNPDKQYLADIIG
                     TYFKAHSSETEPENEGIDFDQRLRRIMKTDRPGDGSGAYAIPENGSRNKKPFFRLNRL
                     LPYAAAITSIGFTVWLVYRSTKPPAVRPANPDARTSQVVSRPGVRPKLVLPDGTQVWL
                     NSGSKLHYKNDFDKSIREVSLEGEAFFDVVKEVDHPFIVHTSAINVRVLGTAFNVKSY
                     PRD"
     gene            complement(731..1333)
                     /locus_tag="VGM24_07680"
     CDS             complement(731..1333)
                     /locus_tag="VGM24_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY4155291.1"
                     /translation="METFNPFSDPSRYPDLLQQIATGNQPAFKQLYGYFYRRLYHFAL
                     SMVKIKEASEEIIEDVFIRIWKQKENMTGIRNLRVYLYTAVKNSSLNYLSKKARESIT
                     EPFDHIEIEFIESVNPEQLMITAELFNKIRQAIENLPPRCKMIFKLIREDGLKYKEVS
                     QILNISVNTIDAQMAIAVSRLAAALTADFGPMARKKPVAE"
     gene            complement(1413..2261)
                     /locus_tag="VGM24_07685"
     CDS             complement(1413..2261)
                     /locus_tag="VGM24_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEY4155292.1"
                     /translation="MLNRFKRNYLFFGIICLCAGCFCGYSVGAQPAVPAFKVIAFYTG
                     RNDQAHISFVHEANRWFPEMAKKYHFSYDSTNDWKNLNADFLSHYQVVIFLDTRADDP
                     AQRDAFKNYMEHGGAWMGFHFAGFALTPSDFPQNWDWYHNEFLGSGSYVSNTWRPVSA
                     ILRVEDKNHPVTRNLPETFKSSPSEWYRWSNDLRTNPDIDILLSIDSSSFPLGTGPKL
                     NEIWHSGYYPVAWTNKKYRMVYFNMGHNDIDYEHKTNKELSHTFHNPDQDQLVLNALL
                     WLGRGK"
     gene            2409..3185
                     /gene="otsB"
                     /locus_tag="VGM24_07690"
     CDS             2409..3185
                     /gene="otsB"
                     /locus_tag="VGM24_07690"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY4155293.1"
                     /translation="MAIFANLVGYGMKELLIENFKRAKNKLILLDYDGTLVDYKTAPQ
                     DATPSKKLLFSLLKLNESPRTKLVIITGRAYQDIDGFVGHLPIDILAEHGAMIRENDE
                     WKELVPDDERWKQTVYPVLNRFIDDCPGSFVEEKRFAMAWHYRNADQETGFSVSRQLI
                     DALAGEIRRLNLKITDGNKVVEIKRKDIDKGRGTVFLLDKDDYDYILAIGDDKTDEDM
                     FVVLADNPHAFTIKVGEGATSAKYRLENVQNVMGLLNELL"
     gene            3182..5410
                     /locus_tag="VGM24_07695"
     CDS             3182..5410
                     /locus_tag="VGM24_07695"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY4155294.1"
                     /translation="MKINLALIISIIAAVGLVAFGFTAFQISSERQQLNSELEAKTIQ
                     VAEAFYKSHFSHLEKEESQHFTNITDSLITQYRFSGMAVCYNNDSVVALNSTAEPYLK
                     YSIDYIAQAISADSSMGNLINVHGKKVYEYIRLIKQRDLPESAVIFYADADYISNIVQ
                     SIWLRNFVRWFLQALVISIVTLLVVKWGILTPLNRVVEWIKAVRFGNADQFKKRPMLN
                     FLEPLYKEISGIAQAMQEAKAIAQEEAQLRTSGEAIWTHERLNEEMKQVLQDKILVVV
                     SNREPYMHVHAGKEIKCIVPASGMVTAMEPILKACGGLWIASGSGDADKDTVDEEDKV
                     QVPPYEGKYTLRRIWLSKEQENHYYYGFSNEGLWPLCHIAHTRPIFRKEDWDYYQEVN
                     ELYAKAVLEEIADQEEPFILVQDYHFALLPKLIKDKRPDAKVAIFWHIPWPNPESFGI
                     CPWQRELLKGMLGGDLIGFHTQYHCNNFLETVNTSLESRVIWENFSVKIGNHFTLVKP
                     FPISISFTLKDYENGNGQKEDPAELLRQHGLHAEFIGIGVDRIDYTKGLIEKFLAIER
                     FLEKYAEYRGKFTFIQIGAPSRSLLKSYADITSAVENEVNRINWKFKSKNWQPILFLK
                     RHHSHEEIIPYYRSAAFCMVTSLHDGMNLVAKEFIAARTENDGVLILSRFAGASQELH
                     GALIINPYDVEQTADAIREALEMPKLLQVQKMKQMRRVIMGHNVYSWASNILRAMASI
                     QN"
     gene            5585..6322
                     /locus_tag="VGM24_07700"
     CDS             5585..6322
                     /locus_tag="VGM24_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155295.1"
                     /translation="MRLRLFLFYLLSVYFCPFSLLAQSASEDSSLYRSALAHTLDTYY
                     SQVGDQSAIYNGRLYSGYGFSFKAGIPYFLSGEFRNESLFYDGIQFENIPLLYDNLSE
                     VLITRDRGYWVQLVNERIGSFTIAGHRFIRIVTDSLNRSLSGTGFYEILYQGHTIVLK
                     KTVKKIREELSSSEGILRFIDSTEHYYIKMGNAYFTVSSRRAMLDIFSDRKSQVRQFI
                     KKNKLKYRKDKENTMVKVTGYYDQITK"
     gene            6325..9102
                     /locus_tag="VGM24_07705"
     CDS             6325..9102
                     /locus_tag="VGM24_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4155296.1"
                     /translation="MKYRILLLLLLFLQACLFPAAAQKNAQNLISGNFQNLTIEQFTQ
                     ELESQTGYYFYYDDSLFDSLRVTLSASREPLSKVLEKAFAGTDFNYAIDRHLQVFLTR
                     GVMVQTNFEAGMPDSVVRKRVRENPEEAADEEERPSNHLQRAILENKLYQIGENAGNN
                     GQATAIIAGYVRDAKTGEPVIGASIYMDKPRIGVASDQYGYYTISLPKGRHILNIQSI
                     GMKDTRRQILLEGDGKMNIDLQGQVMMLKNVIVSAQKLSNVKGMEMGVQKIDIRTIKQ
                     VPVVFGEADLLRVVLTLPGVKSVGEASTGLNVRGGSSDQNLILFNEATIFNPSHFFGL
                     FSAFNPEVVKDVQLYKSSIPARYGGRLSSVLDITSREGNKKNITGSAGIGLLTTRLDV
                     EGPLIKDKSSFILGGRTTYANWLLNLLPQQYKHSKASFYDLNLNVTHQINKKNNLYLT
                     AYTSSDHFNLNSDTAYSYSNLNFSLKWKHNFNDKLYGVFTTGYDRYQYQISSDNNPVN
                     AYKLNFDINQKYLKAHFNYYLNSSHTMEFGLNTINYKLDPGSFLPVGKSSLVAPDVVE
                     AEHALESALYVSDRYTISPSFSVEGGLRYSMFNYLGPQSVNEYAPGIPKTVDNILSTK
                     TYSGGDFIKTYSGPEYRVSVKYEISDSFSIKAGFNTQRQYIHVLSNTAAIAPTDIWKL
                     SDPNIKPQAGSQISLGLYRNFKNNTIETSLEVYYKKIQNYLDYKSGAELVLNHHIETD
                     VINTGGKAYGVELLIKKATGKLNGWISYTYSRILLKMDDPNAGEIINEGKYYPANYDQ
                     PHSATCIANYRVNHRFSVSVNATYSTGRPITLPVGVFFYDNALRTLYADRNAYRIPDY
                     FRTDFSMNIEGNHRVHQKTHNSWTVGVYNVTGRKNPYSVYYVTENGAVNGYKLSIFGS
                     AIPYVNYNIRF"
     gene            9112..>9573
                     /locus_tag="VGM24_07710"
     CDS             9112..>9573
                     /locus_tag="VGM24_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEY4155297.1"
                     /translation="MKAIHWLMLLMVLGCKDKYNAPVHIPATGYLVVEGFINVGSGST
                     DFLLTRATGLDSPYTRAEPGALVSVESENGESYPLAEQGEGKYSISQVPVDFNQRYKL
                     RVRTTDGREYESDLTSPRLTPPIDSVSWKPADGGIWIYVTTHDPAGNSRYYQ"
BASE COUNT         2556 a         2211 c         2393 g         2413 t
ORIGIN      
        1 ggtcccgcgg ataggatttt acgttgaagg ctgttcccag cacccgcaca ttaatcgcgg
       61 aagtatggac gataaaggga tggtccacct cttttaccac atcaaaaaaa gcttctcctt
      121 ccaggctcac ttcccggatg ctcttgtcaa aatcgttttt gtagtgaagc ttgctgcccg
      181 aattcagcca gacctgggtt ccatccggca atacgagttt ggggcgcacg ccgggcctgg
      241 aaacgacctg gctggtccgg gcatccggat tggccgggcg gacggccggc ggtttggtag
      301 atcggtaaac cagccagacg gtaaacccga tactcgttat agcggcggca taaggcagaa
      361 gacggttaag ccggaagaac ggttttttat tccgggatcc attctccgga atggcatatg
      421 ctccggaacc gtcgccaggc ctgtctgtct tcatgatccg ccggaggcgt tgatcaaaat
      481 caatcccctc gttttccggt tccgtttcgg aagaatgcgc cttgaaatag gtgccgatga
      541 tgtctgccag gtattgttta tccggatttt gtttaagtaa aagctgcaat tcttcggctt
      601 cggagacgga agcctcttcc gccaggtggc gggcaaccaa aagccataag cggtcctcgt
      661 tcatgttgca gcagttatct atcagagaca acgaaatgag gtccgtctac taagggaaat
      721 gaaaattaat ttattcagcc acgggtttct tccgggccat cggtccgaaa tcggcggtca
      781 gtgcggccgc caggcgggaa accgcaatgg ccatctgggc gtcgatcgta ttgacggaaa
      841 tattcagtat ctgggagact tctttatact tcaggccgtc ttcccggatc agcttaaaga
      901 tcatcttaca gcggggcggt aagttttcta tggcctgccg gatcttattg aacagttccg
      961 cggtaatcat cagttgttcg ggattgaccg attcgatgaa ttcaatttca atatgatcaa
     1021 agggttcggt gatgctttcc cttgcctttt tggaaagata attcagggag ctgttcttta
     1081 ccgccgtata caggtaaact ctcagattcc ggatgccggt catattctct ttttgtttcc
     1141 agatccggat aaatacatcc tcaataattt cctctgaggc ctcttttatt tttaccatgg
     1201 acagggcgaa atgatacagc ctcctgtaaa aatatccgta tagttgttta aatgcgggct
     1261 gatttcccgt ggcaatttgc tgcagtaagt cggggtacct ggatgggtct gaaaagggat
     1321 taaaggtttc cataagcctc gttttggttc acactccgta aacgcaaaat tatttaaaac
     1381 ttttaagaaa gtacgggaaa ccttcggaaa gatcatttgc cccttcccag ccacaacaga
     1441 gcatttagga cgagctggtc ctggtccggg ttatggaagg tgtgggataa ctctttattg
     1501 gtcttgtgct cgtaatcaat atcgttatgc cccatattaa aatagaccat cctatatttt
     1561 ttattggtcc acgccaccgg gtaatacccg ctgtgccaga tctcatttaa tttgggccct
     1621 gttcccagag ggaaactgct tgagtcgatg gataacagga tgtcaatatc cggattggtg
     1681 cgcagatcgt ttgaccagcg gtaccattca ctcggtgacg atttaaatgt ttccggcaga
     1741 ttgcgcgtta cgggatgatt tttgtcctcc acccggagaa tggcggatac gggcctccag
     1801 gtattgctca cgtaggaacc ggatcccagg aattcattgt ggtaccagtc ccagttctgc
     1861 gggaaatcag agggggtgag cgcgaatccg gcaaaatgaa atcccatcca ggcgccgccg
     1921 tgttccatgt aattcttaaa ggcatcccgc tgggccgggt catccgcccg ggtgtccagg
     1981 aagatcacga cctggtaatg ggatagaaaa tcggcattca ggttcttcca gtcgttggtg
     2041 gaatcgtagg agaaatgata tttttttgcc atctccggaa accagcggtt tgcctcgtgc
     2101 acaaaactga tatgtgcctg atcgttcctt ccggtataaa aggcgatcac tttaaaagcc
     2161 gggacagccg gctgggcgcc gacgctgtag ccgcaaaagc agcctgcgca caggcagata
     2221 atcccgaaga aaagataatt gcgtttaaag cggttcagca tccctgggtt tggggtttgt
     2281 tataaaagta aaggatttat gccgtattta aaccggtgct gaaagagacg gaaaaaaggg
     2341 agcggtaacg gatctgcgga aggcccggag tacaagctat attttgacta aatttgcgca
     2401 tattttattt ggccatattt gcaaatctcg tgggttacgg aatgaaagaa ctattgatcg
     2461 aaaattttaa aagggcaaaa aataagctga tattattgga ttatgacggg accctggtcg
     2521 attataagac ggctccccag gatgcaaccc cttcaaaaaa attgctgttt tccctgctga
     2581 aattaaatga atcgccccgg accaaactgg tcatcatcac tggccgggcc tatcaggaca
     2641 tcgatggatt tgtagggcat ctgcccatag acattttggc ggagcacggg gcgatgatca
     2701 gggaaaatga tgaatggaag gaattggtgc cggacgatga acggtggaag cagacggttt
     2761 atccggtcct caaccggttt atagacgact gcccgggttc ctttgttgag gaaaaacggt
     2821 ttgccatggc ctggcattac cggaatgcag atcaggaaac ggggttttct gtttcgaggc
     2881 aactgattga cgcgctggca ggagaaatcc gccggctaaa ccttaaaata acagacggta
     2941 acaaggtggt ggagatcaaa agaaaggata tagacaaggg cagggggacg gtgttcttac
     3001 tggataagga tgattatgat tatattttgg ccatcggcga tgataaaacg gatgaggata
     3061 tgtttgtggt cctggccgac aatccccatg ccttcaccat taaggtcggg gaaggagcta
     3121 cctcagccaa atacagactg gaaaacgtgc aaaatgtaat gggtctctta aatgaattgt
     3181 tatgaagata aacctggccc tgattatttc gattattgcc gcggtaggcc tggttgcctt
     3241 tggtttcact gcttttcaga tatcctcgga acggcagcag ctgaactccg aactggaagc
     3301 caaaaccatc caggtggcgg aagcttttta taagtctcat ttctcccatc tcgaaaaaga
     3361 agaaagtcag cattttacaa atatcacgga cagcctgatc acccagtacc ggttcagtgg
     3421 aatggccgtg tgttataata atgatagtgt ggttgccctg aacagcaccg cggaacctta
     3481 tctgaagtat tccatcgact acattgccca ggccatttcc gcagattcct ccatgggcaa
     3541 cctgatcaat gttcacggaa agaaagtgta tgaatatatc cggctcataa aacaacgcga
     3601 cctgcccgaa agcgctgtta ttttttatgc agatgcggat tatatctcca atatcgttca
     3661 aagtatctgg ctgcggaatt ttgtgcgctg gttcttgcag gcgctggtca tctccattgt
     3721 caccctgctg gtggtgaaat ggggcatcct gacgccctta aaccgcgtgg tggaatggat
     3781 aaaggccgtg cgcttcggga atgcggacca gtttaaaaag cggccgatgc tcaatttcct
     3841 ggaacccctg tacaaggaaa tttcaggcat tgcccaggcc atgcaggaag caaaggcgat
     3901 cgcacaggaa gaagcccagc tccggacctc cggggaagcc atctggacgc acgaacggct
     3961 gaatgaagaa atgaaacagg tattgcagga caagatcctg gtggtggttt ccaaccgcga
     4021 accctatatg catgttcatg ccggcaaaga aattaaatgt attgttccgg ccagcgggat
     4081 ggtgacggct atggaaccca tcttaaaagc ctgtggcgga ctgtggatcg cttccggaag
     4141 cggggatgcg gataaggata cggttgacga agaggataag gtccaggttc cgccatacga
     4201 aggaaaatat accctgagaa gaatatggct ttccaaagaa caggagaacc attattatta
     4261 cggattttcc aacgagggct tatggccttt atgccatatt gcccacacca ggccgatttt
     4321 cagaaaggaa gactgggatt attaccagga agtgaatgag ctgtacgcca aagcggtcct
     4381 ggaagaaata gcggaccagg aagaaccctt tatcctggtc caggattatc actttgccct
     4441 tttacccaaa ttgatcaagg acaagcggcc cgatgccaag gtggccatct tctggcatat
     4501 tccctggccc aacccggaat cctttggtat ctgcccctgg cagcgggaat tattaaaagg
     4561 tatgctaggt ggtgatctga tcggttttca cacgcaatat cattgcaata attttctgga
     4621 aacggtcaac acctctctgg agtcgcgggt gatctgggaa aatttttccg tcaagatcgg
     4681 caaccatttt accctcgtga aaccttttcc gataagcatc tcctttacct taaaggatta
     4741 tgaaaatggt aacgggcaaa aggaagaccc ggctgaatta ctgagacaac acggtcttca
     4801 tgcagagttc atcgggatcg gcgtggacag gattgattat accaagggcc tgatcgagaa
     4861 attcctggcc atcgagcggt tcctggaaaa atatgccgaa tacagaggaa agttcacgtt
     4921 tatccagatt ggcgctccca gccgttccct cttaaagagt tatgccgata tcaccagcgc
     4981 ggttgaaaat gaggtaaacc ggataaactg gaaattcaag tcgaagaact ggcagcccat
     5041 cctgtttctg aaaagacatc atagccacga agagatcatt ccctattaca gaagcgccgc
     5101 attttgtatg gtcacctctc tacacgatgg catgaacctg gtcgccaaag aatttattgc
     5161 ggcacgcacg gaaaatgacg gcgtactcat tctcagccgg tttgcggggg catcgcagga
     5221 acttcacggg gctctgatca ttaatccgta tgatgtggaa caaacggccg atgcgatccg
     5281 ggaagcgctg gaaatgccaa agctcctgca ggttcagaaa atgaaacaaa tgcgccgggt
     5341 catcatgggc cataatgtgt attcctgggc atccaatatc ttaagggcca tggcctccat
     5401 ccagaattaa cggcatcccg ccgcggaggc caacagccag ggataaacgc tgttcatacg
     5461 ggaaggtcgg tggaaacggg aaaaaatacg gtaaaagcga aagatcatcc gtcttttcag
     5521 tctgttcgtc tcctttattc cctttattta atgaaaaaaa gataacttac caggttttac
     5581 tgctatgaga ctcagattgt tcctgtttta cttattgtct gtttattttt gtcccttttc
     5641 gcttttggcc cagtcggcat ccgaagacag tagtctgtac cggtccgccc ttgcccatac
     5701 gctggacacc tactatagcc aggttggcga tcagtcggct atttacaatg gccggcttta
     5761 ctccgggtac ggattttcct ttaaagccgg cattccctat ttcctgtccg gtgaattccg
     5821 taatgaaagt cttttttacg acgggattca atttgaaaat attccccttc tatatgataa
     5881 cctctcggaa gtattgatta ccagagaccg gggttactgg gtgcagctcg tcaacgagcg
     5941 gatcgggtca tttaccattg ccgggcaccg gtttatccgg atcgtaacgg atagcttgaa
     6001 caggtccctg tccggaacgg gtttttatga aatcctttac cagggtcata ccatagtcct
     6061 taaaaaaacg gtcaaaaaga taagggaaga attatcctct tccgaaggaa tactccggtt
     6121 catagacagc accgagcact attatattaa gatgggtaac gcctatttta ccgtatcctc
     6181 ccggcgggca atgctggata tttttagtga ccgcaaaagc caggttcgtc agtttataaa
     6241 aaagaataag ttgaaatacc gtaaggataa ggagaacacg atggttaaag taaccggcta
     6301 ttacgaccaa ataacgaagt aattatgaaa taccgcattt tattactact tctattattc
     6361 cttcaggcct gcctctttcc ggctgcagcg cagaaaaatg cacagaacct gatcagcgga
     6421 aattttcaga acctgaccat tgaacagttt acgcaggaac tggaaagcca gaccggttat
     6481 tatttttatt atgacgattc cttattcgac agtctccggg ttacgctttc tgccagccgg
     6541 gaaccgctta gcaaggtgct ggaaaaagcc tttgccggaa cggatttcaa ttacgccatt
     6601 gaccggcatc tgcaggtctt tcttacccgg ggagtgatgg tccaaaccaa ttttgaagcc
     6661 ggcatgccag acagtgtggt caggaaacgc gtcagggaga acccggaaga agcagcggat
     6721 gaagaagaaa gaccgtcaaa tcatttgcaa cgggcgatcc tggaaaataa actctatcag
     6781 ataggagaaa atgccggcaa taatggccag gccacggcga ttattgcggg ctatgtccgg
     6841 gatgccaaga ccggcgaacc ggtgatcggc gcttccatct atatggacaa gcccaggatc
     6901 ggggtggcct ccgatcaata tggttactat accatttcat tgcccaaagg acggcatata
     6961 ttgaatattc agagtatcgg tatgaaggat acccgtcgcc agattctgct ggaaggcgac
     7021 ggaaagatga atatcgatct tcagggacag gtaatgatgc tgaaaaatgt gattgtttcc
     7081 gcgcagaaat taagtaatgt caaaggcatg gagatggggg tgcagaaaat cgatattaga
     7141 accataaagc aggtgccggt ggttttcgga gaagccgatc tgctgcgcgt ggtcctcact
     7201 ttaccgggag taaaatcggt tggagaggcc agtaccggat taaatgtccg tggcggatcc
     7261 tcagatcaga acctgatctt attcaatgag gcaacgatct tcaatccttc ccatttcttt
     7321 ggcctctttt ctgctttcaa cccggaggtt gtaaaggatg tacaactgta taagagcagt
     7381 attccggcca ggtacggggg ccgcttatct tccgtgctgg atataaccag ccgcgaaggc
     7441 aataaaaaaa atataaccgg ctctgcggga atcgggctgc tgactacccg gcttgacgtg
     7501 gagggtccgc tgatcaagga caaatcctct tttatcctcg gcggcaggac cacctatgcc
     7561 aattggttat tgaacctgct tccccagcaa tataagcaca gcaaggcttc cttttatgat
     7621 ctcaacctga acgttaccca tcagatcaat aaaaagaaca acttatacct cacggcgtat
     7681 acgagcagcg atcattttaa tctcaacagt gatacggcct acagttacag caacctgaat
     7741 ttctccctga agtggaagca taattttaat gataagctgt acggcgtatt cactaccggg
     7801 tatgaccgct accagtatca gatttccagt gacaataatc cggtgaatgc ctacaaactg
     7861 aattttgaca tcaaccagaa atacctgaaa gctcatttta attattacct gaattcctcc
     7921 cataccatgg aattcggact gaatactatt aattataaac tcgatcccgg aagtttcctc
     7981 cccgtgggta aatcttccct ggttgcaccg gatgtggtgg aagcggagca tgcgttggaa
     8041 agcgcattat atgtcagtga ccgctatacc atcagccctt ccttctcggt ggaaggaggt
     8101 ctgcgttatt ccatgttcaa ttacctcggt ccgcaatccg ttaacgaata tgctcccggc
     8161 atccccaaaa cggttgataa tatattgtcg acgaaaacct attccggagg tgattttata
     8221 aaaacctatt cgggccccga ataccgggtc tccgtgaaat atgaaatatc ggatagtttt
     8281 tccatcaagg ccgggttcaa tacccagcgt cagtatattc atgttttatc gaatacggcg
     8341 gccattgccc ctaccgatat ctggaaatta agcgatccga atatcaagcc ccaggccgga
     8401 agccagatct ccctcggact atacaggaat ttcaaaaaca ataccattga aacttccctg
     8461 gaagtgtatt acaaaaaaat tcagaactac ctggattata aaagcggagc ggaactggtt
     8521 ttgaatcatc atatagaaac agatgtcatc aacaccggcg gcaaagctta cggagtggag
     8581 ttgctgatta aaaaggcgac cgggaagctg aatggatgga tcagttatac ctattcccgc
     8641 atattgctta aaatggacga tcccaatgcg ggtgaaatta tcaatgaagg gaaatattat
     8701 ccggccaatt acgaccagcc gcattcggcc acctgtattg ccaattaccg ggtcaatcac
     8761 cggttcagcg tatcggtgaa tgccacctac agtacgggtc gtccgatcac ccttccggtt
     8821 ggtgtatttt tttatgacaa tgcgctcagg accttgtatg cagaccggaa cgcttaccgg
     8881 attccggact atttccgaac cgatttttca atgaatatcg aagggaatca ccgcgttcac
     8941 cagaagaccc ataattcctg gacggttggc gtgtacaatg ttaccgggag aaaaaatcct
     9001 tattcggttt attatgttac ggaaaacgga gcagtgaacg ggtataagct ttcgattttt
     9061 ggcagcgcca ttccttatgt caattacaat atccggtttt aaaaagggtt tatgaaagcg
     9121 attcattggt tgatgctgtt gatggtgctg ggctgtaagg ataaatataa cgcccccgtt
     9181 catatcccgg caacgggcta cctggtcgtg gaaggcttta tcaatgtggg aagtggttca
     9241 accgatttcc tgctgacccg ggctacgggt ctggactccc cgtatacccg cgcggaaccg
     9301 ggcgccctgg taagcgtgga atcggaaaac ggagaaagct atccgctggc cgaacaggga
     9361 gagggaaaat attccatcag ccaggtgccg gtggatttca atcagcggta caagctgcgg
     9421 gtccggacta cggacggcag ggagtatgaa tcggatctta cttctccccg gcttactccc
     9481 cccatcgaca gcgtgagctg gaagccggcg gacggcggca tctggatata tgtgaccacg
     9541 cacgacccgg caggaaattc ccggtattat caa
//
LOCUS       DASYCU010000381         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2402316, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000381 DASYCU010000000
VERSION     DASYCU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402316"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VGM24_07715"
     CDS             <1..796
                     /locus_tag="VGM24_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY4155298.1"
                     /translation="TYRSKYLIMATGLYGKPRAVSFKGMETFPGKILHSYEYKTGKDF
                     KGQDVLVVGFGNSACEIAIDLYEQGAVPSMAVRSAVNVIPRDVLGVPVLKLSLMLKRL
                     RPRLADRLSAPLISWLIGDLSSLNLKKMPYGPLEQIRRDGKAPVLDIGTIRHIRQTHI
                     KIYDNIDHIEGETVHFKDGKKGNFDVIVAGIGYHRDGIGIVDVDSRRIADLQMSADKQ
                     KYFGNEGLYFCGYWVSPTGQIREIASDAKKIAGDIAKRENHSGDHR"
     gene            1038..2195
                     /locus_tag="VGM24_07720"
     CDS             1038..2195
                     /locus_tag="VGM24_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155299.1"
                     /translation="MNIQLKAQKTIPALLFLSVFMFSDRAIGQTDSTAKDSALLKQIE
                     LQMQSSTPTPPQQTRSGISANPDIGLVADFRGSYISHGKRNFDAYLDETEISFQSVVD
                     PYIRADFFVSFERDPVTHNYGVSVEEGYLTSLSLPAKLQLKVGKFREAVGKINPTHPH
                     ALPFIDLPNAFVNYFGEEGLNDEGASLSWLLPTKAFYQELVFQATSGAGESPTFYRGD
                     NNRFIYLGHLKNFFTLTDNATLELGLTGISGPNDSSSYTNIGAVDLTYKWKPVQMNTY
                     KSLTWQSEFFYSSAKYSRNEMSNSLGLYSFLEYQIAKRWFLTGRYDYAEKPYDRDILE
                     QAYSLSAGWLATEFQKIEFEGKLTHDNIQPTYDQFWLRWIFVIGAHGAHQY"
     gene            2211..>2918
                     /locus_tag="VGM24_07725"
     CDS             2211..>2918
                     /locus_tag="VGM24_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEY4155300.1"
                     /translation="MNRVILIFSSLLMLVSGADAATIKVVTTTMDMKSIAEMIGGNKV
                     SVQSIATGYQNPHFVDPKPSYIIGLSNADLFVTVGLDLETGWSPQLLASSRNSRIQKG
                     SPGYVDASEGVALYQVPSVVNRGEGDIHIYGNPHYWLDPLNGKVIARNIANGLERIDP
                     SGRAFYEANLKTFSDKIDQKLKQWAAEMAPFKGTKIIAYHNEWVYFEKRFGLQIVDFM
                     EPKPGIPPSPSQLVKVIH"
BASE COUNT          759 a          670 c          730 g          759 t
ORIGIN      
        1 cacctaccgg tcgaaatacc tgatcatggc cacggggctt tatggtaaac cccgggcggt
       61 tagctttaaa ggaatggaaa catttcccgg gaagatcctg cacagttacg aatataaaac
      121 aggcaaagat tttaaaggac aagacgtttt ggtagttggg tttggcaatt cagcctgtga
      181 aattgcgatc gatttatatg agcagggtgc ggttccttcg atggcggttc gctctgcggt
      241 aaatgtaatc ccgcgggatg ttttgggcgt cccggttctg aaactgagtt taatgctgaa
      301 acgtctccgg ccacgcttgg ccgacaggct cagcgctcca ttgataagct ggctgatagg
      361 cgatctttcc agcctgaatc tcaaaaaaat gccctacggc ccgctggaac aaattcgccg
      421 ggatgggaag gctccggtgc tcgacatcgg caccatccgg catatccgcc agacccatat
      481 aaaaatctat gacaatattg atcacatcga aggggaaacc gtccatttca aagatggtaa
      541 aaaaggaaat tttgatgtga ttgttgcggg catcggatat caccgcgatg gtatcgggat
      601 tgttgatgtg gatagtcgcc ggatcgcgga tttgcagatg agtgcggata aacaaaaata
      661 ttttggcaat gagggtcttt atttctgcgg ttactgggtc agcccgacgg gccagatccg
      721 ggagattgcg tccgatgcaa aaaaaattgc cggggatatt gctaaacgag aaaaccattc
      781 gggagatcac cgttgacagt tcatccggtt catgccggta aaaatgattt atcaggacgt
      841 attggtctcc gtcaattatt cttaacttac atgttttctc atcctgaatc ttctctgaat
      901 tatgttcggc ggaaaattta ccggcagcgc aaaggcaatc acagatacag gcagggtacc
      961 agtcgatgga cccggcagaa cgccgggacg gagttgttat gagatttact gcaatgggtt
     1021 caaaaacaaa tattcacgtg aacattcaac ttaaggctca aaaaacaatt ccggccctgt
     1081 tatttctttc cgtatttatg ttcagtgacc gggccattgg gcaaacggac agtacggcta
     1141 aagattccgc cctgcttaaa cagatcgagc tgcagatgca gtccagtacg ccaactcctc
     1201 cccagcagac ccgttccggg atttcagcca acccggatat cggtttggtg gccgatttcc
     1261 gggggtccta tatcagccac ggcaaaagga attttgatgc ctatctcgat gaaacggaga
     1321 tctcctttca atctgttgtg gatccctata tccgggcaga cttttttgtt tctttcgaac
     1381 gagatcccgt gacacataat tatggagtgt ctgtggaaga aggttatctc acctccctct
     1441 cactgcccgc gaaactgcag ctgaaagtgg gaaaattcag ggaagcggtc ggaaaaataa
     1501 atccgacgca tccgcatgct cttcctttta ttgatctgcc caatgccttc gtaaattatt
     1561 ttggagagga aggcctgaat gatgaaggag cgtccctgag ctggcttctt cccaccaagg
     1621 ctttttacca ggaactggtt ttccaggcca cttcgggagc cggtgaatcg ccgacttttt
     1681 accggggcga caacaatcgt tttatttatc ttggtcacct taagaacttt ttcaccctta
     1741 cggataatgc cacgctggaa ttgggtttga ccggtatttc tggacctaat gattcttcca
     1801 gctacaccaa tatcggtgct gttgatctga cgtataagtg gaagccggtt cagatgaata
     1861 cgtataagtc tcttacctgg caaagcgagt ttttttacag cagcgccaag tattcgagaa
     1921 atgaaatgtc taacagcctc ggcctttatt ctttcctgga ataccagatt gcgaaacgct
     1981 ggttcttaac cggcaggtat gattatgccg aaaaacccta cgacagggat atcctggaac
     2041 aggcgtattc gttgagtgca ggctggctgg ccaccgagtt tcagaaaatc gaatttgagg
     2101 gtaagctgac ccacgataat attcagccga catacgacca gttctggctg cgctggatct
     2161 ttgtgatcgg cgcgcacggg gcacatcaat attaaaaaag acaataaatc atgaacagag
     2221 ttattttaat tttttcctcc ctgctgatgc ttgtttccgg tgcggatgcc gccaccataa
     2281 aggtggttac gacaacgatg gatatgaaga gcattgcgga aatgatcggc ggtaataaag
     2341 tttccgttca atccatcgct acgggctatc agaatcccca ctttgttgat ccaaaaccga
     2401 gttatatcat cggtctttcc aatgctgatc tttttgtaac ggttgggctc gacctggaga
     2461 ccggctggtc cccccagctg ctggccagct cgcgaaattc gagaatccaa aagggctctc
     2521 ccgggtatgt ggatgcatcg gaaggagtgg ccctgtatca ggtgccttct gttgtgaacc
     2581 ggggggaagg cgatattcat atttatggaa atcctcacta ctggctggat cccctgaacg
     2641 gaaaggtgat tgcgagaaat attgcgaatg gcctggaacg gatcgacccc tccggcaggg
     2701 cattttatga ggcgaatctg aaaacctttt ccgataagat cgaccagaaa ttaaagcaat
     2761 gggcggcaga aatggcgccg tttaaaggaa ccaaaatcat tgcgtatcac aatgaatggg
     2821 tctattttga aaaaagattc ggtttacaga tcgtggactt tatggaaccc aaaccgggca
     2881 ttcctccttc gccctcccag ttggtgaaag tgatccat
//
LOCUS       DASYCU010000382         4344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1825525, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000382 DASYCU010000000
VERSION     DASYCU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825525"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VGM24_07730"
     CDS             <1..1566
                     /locus_tag="VGM24_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEY4155301.1"
                     /translation="YLHIGHAKSICLNFGLAQKYGGKTNLRFDDTNPAKEETEYVDSI
                     LEDVKWLGFQPAQVVYASDYFEKLYSYALILIQKGLAYVDDSSSEEIARQKGTPADPG
                     VENKFRNRSVEENDKLFQEMREGKYRDGEKVLRAKINMASPNMHMRDPILYRIKHTHH
                     HRTGDSWCIYPMYDFAHGQSDSIEQITHSICTLEFVPHRPLYDWLIEKLEIFPSRQYE
                     FARLNLNYTIMSKRKLLQLVNEKYVTGWDDPRMPTISGLRRRGYTPESIRSFADRIGV
                     QKRDNLIDVSLLEFCIREQLNRTAMRKMVVFDPLKIVISNYPDTVEMLSSEDYPEGAE
                     QSSHRELPFSRELYIEREDFMENPPKKFFRLAPGRRVRLKSAYIIQCDEVIKDDTGKI
                     TELRCSYLPESRSGTDVSGLKVQGTLHWVSLPHAINIELRLYDRLFKTENPATGEGDF
                     QEYINPDSLRVVSHAFAEPSLANAKFDERYQFIRKAYFCLDKDSSEEKMIFNRTVTLK
                     DGWAKEIRKVDGI"
     gene            complement(1603..1959)
                     /locus_tag="VGM24_07735"
     CDS             complement(1603..1959)
                     /locus_tag="VGM24_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155302.1"
                     /translation="MKQLIAGAFVLMALFSSVNSFAQQRDPAAREAEMRQKLKTDLKL
                     TDIQIDSVVAINREFMPQRREIYQDQSLSADDKKTKMKAVTDQSDTRIQAVLGDDLFK
                     QYQDWRMKNMQRRRND"
     gene            2097..2912
                     /locus_tag="VGM24_07740"
     CDS             2097..2912
                     /locus_tag="VGM24_07740"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY4155303.1"
                     /translation="MKQYLDLLRHVLEKGVSKSDRTGTGTLSCFGYQIRFDLQAGFPL
                     VTTKKLHVKSIIYELLWFLRGETNTRYLKEHGVSIWDEWADEQGELGPVYGKQWRSWE
                     GADGKTVDQITDTIRQLKKNPDSRRIIVSAWNVAELPQMALMPCHALFQFYVAPPPAA
                     GGRGSLSCQLYQRSADVFLGVPFNIASYALLTLMVAQVCDLEPGEFIHSFGDVHLYNN
                     HLEQAKLQLSRTANPLPRMKLNSGVNNIFEFQFEDFTLENYQPHPPIKAPVAV"
     gene            2923..3438
                     /locus_tag="VGM24_07745"
     CDS             2923..3438
                     /locus_tag="VGM24_07745"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY4155304.1"
                     /translation="MTLSIIVAASENNVIGKNNQMVWHLPEDFRFFKNKTWGLPLIMG
                     RKTYEAMGARQLAGRFNIIITRQRGYPASGPGTQVAGNLEEAIRLAKQTDSKEAFIGG
                     GGQLYEASILLCDKIYITRVKAVLDGDAFFPEIPRETFEMTGSRLVGADEKNAYAMDF
                     QTWEKKRPSRV"
     gene            3456..4247
                     /locus_tag="VGM24_07750"
     CDS             3456..4247
                     /locus_tag="VGM24_07750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4155305.1"
                     /translation="MYSSTRLAFNYLNYLRRSSNGKGHGIHSPFVYEFVTEVLNDERE
                     FYPFAEIESRRSELERDHSLIEVEDYGKSPRSVRNTEVAGIARRAVSTPKFGRLLFRM
                     VNHYQPAQVIELGTSLGISGAYLASANRGSGLITLEGSASIARVAKETFNKLNLPQIR
                     QITGNFDETLSAVIASAPPAGLVYIDGNHRKGPLLNYFQQFLNKRPATAVFILHDIHW
                     SPEMEDAWRIIRDCPELMLTIDLFSAGLVFFRDTFRVKQHFTIRF"
BASE COUNT         1157 a         1047 c         1100 g         1040 t
ORIGIN      
        1 tacctgcata tcgggcacgc caaatccatt tgtctgaatt tcggcctggc ccaaaaatat
       61 ggaggaaaga ccaatctccg tttcgatgat accaatcccg caaaggaaga aaccgagtat
      121 gtggacagta tcctggagga cgtgaaatgg ctcgggttcc agcccgcgca ggtcgtttat
      181 gcttccgatt atttcgaaaa actgtatagc tatgccttaa tccttattca aaaaggactc
      241 gcttacgtgg atgacagcag cagtgaagaa attgcccggc agaaaggaac gccggcggat
      301 cccggcgtgg aaaataaatt ccggaaccgt tctgtggagg aaaacgacaa gctttttcag
      361 gaaatgcggg aagggaaata cagggatggt gaaaaagtat tgcgcgcgaa aataaatatg
      421 gcctccccca acatgcatat gcgggacccg atcttatacc gcattaagca tacgcatcat
      481 caccggacgg gtgactcctg gtgtatttat ccgatgtatg attttgccca cggacaaagt
      541 gattccattg agcaaatcac ccattccatc tgcacgctgg agttcgttcc gcaccggccg
      601 ctgtatgatt ggctcatcga aaagctggag attttccctt ccaggcaata tgaattcgcc
      661 cggctcaacc tcaactacac gataatgagt aagcgaaaac ttttgcagct ggtaaacgaa
      721 aaatatgtga ccggatggga tgacccgcgc atgccgacca tctccggatt gaggcggcgg
      781 ggatatacgc cggaaagcat ccggagtttt gcggaccgga tcggcgttca aaaaagggat
      841 aacctgattg atgtttccct gctcgagttt tgcataaggg agcagctcaa cagaacggcg
      901 atgcggaaaa tggttgtttt tgatcccctg aaaatcgtta tcagcaatta cccggatacg
      961 gtggagatgc tcagcagtga ggactatccg gaaggagctg agcaaagcag ccaccgggaa
     1021 ctgccgttta gccgggaatt gtacatcgag cgggaggact ttatggaaaa cccgccaaag
     1081 aaattcttcc ggctcgcgcc gggcagaaga gtgcgcctga aaagtgccta tatcattcag
     1141 tgcgatgaag tgatcaaaga cgatacagga aaaataacgg aactccggtg cagctattta
     1201 ccggaaagca ggagcggaac cgatgtctcc ggcttaaaag ttcaggggac cctgcactgg
     1261 gtaagccttc cccatgccat aaatattgaa ctgagattgt acgaccgcct gtttaaaaca
     1321 gaaaatcccg caaccggaga aggggatttt caggaatata tcaacccgga ctccctgcgg
     1381 gtggtttctc atgcatttgc tgaaccttcc ctggcaaatg cgaaattcga tgaacgctac
     1441 cagtttatcc gcaaagccta tttctgcctg gataaagaca gttcggaaga aaagatgatc
     1501 ttcaaccgga cggtaaccct gaaggacggc tgggcgaaag aaataagaaa agttgatggt
     1561 atataaaata ccatcaacct gaataagtct gctgctcaag cgtcagtcgt tcctgcgccg
     1621 ctgcatattc ttcatccgcc aatcctggta ttgtttgaaa agatcgtctc ccagcacggc
     1681 ctggatcctc gtatcggact ggtcggtaac ggccttcatt tttgtttttt tgtcatctgc
     1741 gctcagggac tgatcctggt aaatctcccg ccgctgcggc atgaattccc ggttgatcgc
     1801 aacgactgaa tctatctgaa tatcagtcag tttgaggtct gttttcaatt tttgcctcat
     1861 ttcggcttcc cgggctgccg gatcccgctg ctgggcaaag ctgttgacgg aggaaaacag
     1921 ggccatcagt acaaaagcgc ctgctataag ttgtttcata tctgaagttt tgttaggcaa
     1981 ttaatggagc caaatacatg ccagatccct ggatctatgc ctgcattatg caaacggcaa
     2041 aacggtatat tccaccggat caataagaca tttaagaact tcagtaaatt gcgctcatga
     2101 agcaatacct ggaccttctc cggcatgtat tagaaaaggg agtcagcaaa tcagacagga
     2161 ccggtaccgg aaccctcagt tgtttcggat accagatacg gtttgatctc caggccggct
     2221 ttcctctggt gaccaccaaa aagcttcatg tgaaaagtat tatttacgag ctgctctggt
     2281 tcctgagagg ggagaccaat acccgttacc tgaaggaaca tggagtcagc atctgggatg
     2341 aatgggctga tgaacagggt gaactgggac cggtttatgg aaaacaatgg agaagctggg
     2401 aaggggcaga tgggaaaacg gttgatcaga tcacggatac aatccggcaa ctgaagaaaa
     2461 atccggacag ccgccggatc atcgtgagcg cctggaatgt ggctgaactt ccgcaaatgg
     2521 ccctgatgcc ctgtcacgct ttatttcaat tttacgtggc tcccccgcct gcggcaggcg
     2581 gcaggggaag tttaagctgc cagctttacc agcgcagtgc cgatgtattc ctcggagtac
     2641 ccttcaatat tgcttcttat gccttgctga ccctgatggt tgcccaggtc tgcgacctgg
     2701 aacccgggga attcattcat agttttggag acgttcacct atacaataat catctggaac
     2761 aggcaaaact tcagctgtcc cggacagcca atcccttacc ccggatgaaa ttaaatagcg
     2821 gggtaaataa tatattcgag tttcagtttg aagatttcac gctggaaaat tatcaacctc
     2881 atccgcccat caaagcgccg gtagcggttt aaaccagacc tcatgacgct ctccatcatc
     2941 gttgccgctt ctgaaaataa tgtgatcgga aaaaataatc aaatggtttg gcatcttccg
     3001 gaggattttc gttttttcaa aaataagacc tggggattgc cgctgatcat gggaagaaag
     3061 acgtacgaag cgatgggtgc ccggcaactg gcgggaagat tcaatataat catcacccgg
     3121 cagcgcggtt atccggcctc cgggcccgga acacaggtcg cgggaaacct ggaggaagcg
     3181 atccggctgg caaagcaaac cgatagcaaa gaggcattta tcggcggcgg aggccagctg
     3241 tatgaagcgt ccatcctgct ctgtgataag atttacatta cccgggtgaa ggcggtcctg
     3301 gacggagatg cgtttttccc ggaaatcccc cgcgaaacgt ttgaaatgac cggttcaaga
     3361 cttgtgggcg cagacgaaaa aaatgcctat gcaatggatt ttcaaacctg ggaaaaaaaa
     3421 cggccatccc gcgtttagac ggctgatact agaccatgta ttccagcacc cggcttgcct
     3481 ttaactattt aaattatctc cggcgttcgt ccaatggaaa aggacacggg atccattctc
     3541 ctttcgttta tgaattcgtg acggaagtgc tgaacgatga acgggaattc tatccttttg
     3601 cggaaattga aagccggcgt tccgaactgg aaagagacca cagcctgata gaagtggaag
     3661 actatggaaa atcgccccgg tccgtaagga atacagaagt ggccggcatc gcccgccggg
     3721 ccgtttccac tccgaaattc gggaggcttc ttttccggat ggtaaatcat tatcagccgg
     3781 cccaggtcat tgagctggga acctccctgg gcatttcagg cgcctacctg gcttctgcca
     3841 atcggggttc gggcctgatt acgctcgaag gttcggcctc catcgcccgg gtcgcaaaag
     3901 aaacgtttaa caagctcaac ctgccgcaaa tccggcaaat caccggtaat tttgatgaaa
     3961 ccctgagtgc ggttatcgct tcggctccgc cggccggtct ggtttatatt gacggcaatc
     4021 accggaaagg accgctgttg aattattttc agcagttcct gaataaaaga ccggcaacgg
     4081 ctgtttttat tttgcacgat atccactgga gcccggaaat ggaagatgcc tggaggatca
     4141 tccgggactg cccggagctg atgttaacca tcgacctgtt ttccgccggt ctggtttttt
     4201 ttcgggatac attccgggtt aaacaacatt ttaccatccg attttaaata acaaaatgtt
     4261 tcgtttatta gtgactgtta ttctttcccc gctgatcttt tctgcaaagg cccaggattt
     4321 ttcagcttat cagaaaaaaa cctt
//
LOCUS       DASYCU010000383         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4142517, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000383 DASYCU010000000
VERSION     DASYCU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4142517"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1067
                     /gene="queA"
                     /locus_tag="VGM24_07755"
     CDS             18..1067
                     /gene="queA"
                     /locus_tag="VGM24_07755"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY4155306.1"
                     /translation="MKLSQFKFDLPLNLIAQHPAKRREDSRMMVVHRKTGQIENKHFR
                     DVMDYFDDKDVFVVNNTKVFPARMYGRKEKTGAKIEVFLLRELNKPNRLWDVIVDPAR
                     KIRVGNKLYFGDNDELVAEVIDNTTSRGRTIRFLWDGTDDEFRQVLEMLGETPLPKYI
                     KRKPDEEDKERYQTVYAKHEGAVAAPTAGLHFSKELIKRLEIKGIRFAEVTLHTGLGT
                     FRPIEVEDLSKHKMDAEYFRIDEEACRIVNKARMGSNRICSVGTTTMRALESSFTAEK
                     LLKPSEGWTNTFIHPPYQFNIADALITNFHLPKTSLLIMVCAFAGYDLTMEAYKRAIK
                     DKYRFFSYGDAMLLV"
     gene            1198..>2522
                     /locus_tag="VGM24_07760"
     CDS             1198..>2522
                     /locus_tag="VGM24_07760"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEY4155307.1"
                     /translation="MKFDFPRKIRSGYLIAFLLLLFSYFLSISALVQLREQNQWVDHS
                     REVINKLQVLNSYIKDSGIGWRDYIILGDEKYLQPYLKSRAKIDSVCRDLSFFVKDNP
                     QEKERVDLLRHTIDRQFHNNRDQIEAFKNKPFQVSELFRTRTLNSEMLIDSIGLISRS
                     IGSREDELLNLRIRRVGFSQKIVFGIIVAAGIVSGLLILYSLITFNMESQAKKKATLQ
                     AASYHDQLEKRIKELDLANRELIELRSLERFTSTGQIARVIAHEVRNPLTNIDLSAGH
                     LENASLTGEEKKMFLDIITRSSKRINQLINELLTATKFSELEYERIMANELLEESLSE
                     ARDRMKLSQVLIEKKYAETGILLNVDRAQMKIALLNIIVNAIEAMPADEGKLVVETAI
                     VKGNCQIKIRDNGKGIEPEIQPKIFDPYFTSKSKGNGLGLTNTQNIILN"
BASE COUNT          740 a          583 c          605 g          594 t
ORIGIN      
        1 tataaaattc tgattgaatg aagctttccc agttcaaatt cgaccttccc ctaaatctta
       61 ttgcccaaca tccagccaaa cggagagaag acagccgaat gatggtcgtg caccgcaaaa
      121 ccgggcaaat agaaaataaa cactttcgcg atgtcatgga ttatttcgac gacaaggatg
      181 tgttcgtggt caacaatacc aaggtatttc ccgcgaggat gtatggccgg aaagaaaaaa
      241 caggggccaa gatcgaagtt ttcctgctga gggagctcaa taaaccgaac cgcctatggg
      301 atgtcatcgt ggatccggcc cggaaaatcc gagtgggaaa caagttgtat ttcggcgaca
      361 acgatgaact ggttgccgag gtaatcgaca acaccaccag ccggggccgt acgatccggt
      421 ttttatggga cgggacggac gatgaattcc gccaggtatt ggaaatgctg ggcgaaacac
      481 ctcttccgaa atacatcaaa cgcaaacccg atgaggagga taaagaaaga tatcaaacgg
      541 tatatgccaa acatgaagga gccgtcgctg cccccacggc cgggctgcat tttagtaagg
      601 aattaatcaa gcggctggag atcaaaggta tccggtttgc ggaagtcact ttacacaccg
      661 gcctcggaac cttccggccc attgaagtgg aggatctcag taaacataag atggatgcgg
      721 aatatttccg gatcgacgag gaggcctgcc ggattgtaaa caaggcccgg atgggcagta
      781 accggatctg ctccgtcggc accaccacca tgcgcgcact ggagtcctct tttactgccg
      841 aaaaattatt gaaaccctcc gagggatgga cgaacacctt cattcatcct ccttatcaat
      901 ttaatattgc agatgcgctc atcaccaatt ttcaccttcc caaaacgagc ctcctgatca
      961 tggtttgcgc ctttgcagga tatgatctca ccatggaagc atataaaaga gccatcaagg
     1021 acaaataccg gtttttcagt tatggcgatg ccatgctgct cgtttgataa aaacggactt
     1081 ccggatcggc tttagataat tttatgtaat ttcagctact gcaggaaaag tgatcccagc
     1141 agaaatatct gttggctgtt gactggcttc cgggtttttt cttttccgca ttggcttatg
     1201 aaatttgact tcccaagaaa aatccggagt ggttatctga tcgctttcct gttattgctt
     1261 ttctcctatt ttctttccat atctgccctt gttcagctca gggaacaaaa ccaatgggta
     1321 gatcattccc gggaagtgat caataaactt caggtgctga attcctatat taaagactcg
     1381 gggatcgggt ggagagatta tatcatcctg ggagacgaaa aatatcttca gccttacctg
     1441 aaaagccgcg cgaaaattga ctcggtctgc cgggaccttt ccttttttgt aaaagacaat
     1501 ccccaggaaa aagaaagggt tgatttacta aggcatacca tagaccggca atttcataac
     1561 aaccgcgatc agatagaggc tttcaaaaac aagccgtttc aggttagcga gctgttcagg
     1621 accaggaccc tcaacagcga aatgctcata gattccattg gcctcatttc ccggagtatc
     1681 gggtcgcgcg aagacgaatt attgaatctc cggatacgga gggtgggctt ttcccagaaa
     1741 atagtatttg ggatcatcgt ggcggccggg attgtttccg gattgcttat cctgtattcg
     1801 ctgattacat ttaatatgga aagccaggca aaaaagaagg ccactctgca ggcagccagt
     1861 tatcacgacc agctggaaaa acggataaag gaactggatt tagcaaaccg ggaactgatt
     1921 gagctgcgaa gcctggaaag gttcaccagc accggacaga ttgcccgggt gatcgcacac
     1981 gaagtgagaa atccccttac gaatatcgac ctctccgccg gccacctgga aaatgcgtca
     2041 cttacgggcg aagaaaaaaa aatgttcctg gatattatca cgcgcagttc aaaacgcata
     2101 aaccagctga tcaatgaatt gcttaccgcc actaagtttt ctgaactcga atatgaacgg
     2161 atcatggcga atgaattgct tgaagagtcc ctcagtgagg ccagggaccg gatgaagctg
     2221 agccaggttc tcatagaaaa aaaatatgcg gaaaccggca ttttgttaaa tgtggaccgg
     2281 gcccagatga agatcgccct gttgaatatt attgtaaatg cgatcgaagc catgccggcc
     2341 gatgagggaa agctggtggt ggaaacagcc atcgtcaaag ggaactgcca gattaaaata
     2401 cgcgacaatg gaaaaggaat tgaaccggaa atacagccca aaatatttga cccttatttt
     2461 acttccaagt ccaagggcaa cggcctcggc cttacgaaca cacagaatat catcttaaat
     2521 ca
//
LOCUS       DASYCU010000384         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3274786, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000384 DASYCU010000000
VERSION     DASYCU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274786"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGM24_07765"
     CDS             complement(<1..414)
                     /locus_tag="VGM24_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4155308.1"
                     /translation="MVNNSTERFSNRVNDYVKYRPGYPAAIVNYLQDHFDLTIDKQIA
                     DIGSGTGISTALFLDAGYPVRAVEPNKEMRKKSVELLNHYPKYTAVDGKAENTTLPAG
                     SVDAVVAGQAFHWFDAEKAKAEFKRILKPAGLVVLI"
     gene            complement(500..970)
                     /locus_tag="VGM24_07770"
     CDS             complement(500..970)
                     /locus_tag="VGM24_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY4155309.1"
                     /translation="MKEISPLPILRIYRFPMVLLILMVMLNISCSRSKSSDPSGGPTL
                     VNYSGTFVASGGQDSTKAGGTVKATFSSSTRVLTYSITWSSLSSNPVAMHFHDDGPVI
                     VTLSGFPVSKTGSLDGTATLTPSQVSDLSAGLIYAMIHTEIYSAGEIQATLVKQ"
     gene            complement(1137..1940)
                     /gene="xth"
                     /locus_tag="VGM24_07775"
     CDS             complement(1137..1940)
                     /gene="xth"
                     /locus_tag="VGM24_07775"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY4155310.1"
                     /translation="MDLRVFTDHFTMKITTYNVNGVNGHLAVLLRWLGETKPDVVCLQ
                     ELKAPHEKFPEKAIQDAGYNAIWHGQKSWNGVAILARDREIREVRRALPGDPEDIHSR
                     YIEAVINGITIGCLYLPNGNPAPGPKFDYKLNWFERLTAHAAGLLASDIPVVLTGDYN
                     VIPTELDVYKPERWVDDALFRPESRAAFGKLVEQGWTDALRKLYPDQKIYTFWDYFRN
                     AYGRDAGLRIDHFLLSPHLNKRLAAAGVDRNVRGWEKTSDHAPVWIELS"
     gene            2169..>3838
                     /locus_tag="VGM24_07780"
     CDS             2169..>3838
                     /locus_tag="VGM24_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY4155311.1"
                     /translation="MTDLQTSIRLISEICEEQPEAAVWVKPVWSTDGYPDGRKIVDFQ
                     FHYCNPAILDLLGKPAEQIIGKCVLDDRLPDPAVYQIIFDQCREVYETGRSIQYSYLS
                     KGLGKYISLQRVRAHDGVLTTARNRTGEFKLLEEREGQARLLENLVEHSPYGISLYES
                     MRDGKGEITDFKLKLCNRKSSEITGLSLEELFGKTVKELMQLRGHSVYFDICKRVVEK
                     GDPHYMEYYSTSRDQWLGFSFVKFGDGYLLNYIEITQTKKYEQEAKRNAQELSAIFNG
                     SLNGIFSAKLVRDPEGQIFDLIFLRANESFYRMFPETSGQLIGQSLLAISEKEDQSLF
                     LKYVDEVISSGKPAIHVLHYDNSGRWFELSMAKLEEEIISVTLNDITAQKLALLEIER
                     QKNLLAGIMKQSPSGLAVMKAIRDQDGKIKDAVPILLNEACERLLGVPNEILLAGTIG
                     RLEPSLPESSIFDAAGRLAAGESIRTEYYQATTCRWLELAIGKMDEDHFINVLTDITE
                     IKETQQQLEASVADLKRSNENLEDFAYAASHDLKEPIRKIRYYAEQLK"
BASE COUNT          969 a          908 c         1002 g          959 t
ORIGIN      
        1 aatcagtacg accagcccgg caggtttcaa tatcctttta aattcggcct ttgctttttc
       61 cgcatcaaac caatgaaatg cctgaccggc gaccaccgca tccacactgc cggcaggaag
      121 ggttgtattt tcagctttgc cgtcgaccgc ggtatatttt ggataatggt ttaaaagctc
      181 aaccgatttt ttcctcatct ctttattggg ttccacggcc cggaccggat atccggcatc
      241 caggaacaaa gcggtcgata tccctgttcc ggatccgata tcggctatct gcttatcaat
      301 ggtcagatca aaatgatcct gcagataatt tacaatggcg gccggatagc ctggcctgta
      361 tttgacatag tcattcacac ggttgctgaa ccgctccgta ctgttattca ccattggcca
      421 aaattaataa acccccggat atccttaact ccgctggaag ggatggcagg tcattccgca
      481 ggatcttcct taaaacagat tattgtttga ccagggtggc ctggatctcg ccggctgaat
      541 aaatttcggt atggatcatg gcataaatca gaccagcgga gagatccgaa acctgggaag
      601 gcgtaagcgt agctgtccca tccaaactgc cggttttaga aaccgggaat ccggacaagg
      661 ttacaattac cggtccgtcg tcatgaaaat gcatggccac cggattactg gacagggaag
      721 accaggtgat ggagtaggtg agaacccggg tggagctgct gaaagttgcc tttacggtgc
      781 ctccggcttt ggtcgaatcc tgccctccgg acgcaacgaa ggtcccggaa taatttacca
      841 gcgtcgggcc gccggatggg tcgctgcttt tggatcggct gcaggaaata ttcagcataa
      901 ccatcaggat tagcaagacc atcgggaacc ggtagatgcg gaggatgggc aaaggggaga
      961 tttctttcat agcaggcatt tgtagggaaa ggcaaacaat aattcaggtc cattaccccg
     1021 ccttatataa tacgtaatga gccggaaaca gttgcgttgg tcagacggtt gcatggccac
     1081 ttcactggcg gaagcctctt cctttaccgg cattacaaat cattttcttc atcggatcaa
     1141 ctcagctcga tccagaccgg ggcgtgatcg cttgtctttt cccatccgcg aacattgcgg
     1201 tcgactccgg ctgcggcgag gcgcttattc aggtgggggc tgagtaaaaa atgatcaatg
     1261 cgtaaaccgg catcgcggcc ataggcattg cggaaataat cccaaaaggt atagatcttc
     1321 tggtccggat agagttttcg caaagcgtct gtccatccct gttctaccag cttcccgaat
     1381 gcggcgcggg attccggccg aaagagcgcg tcatccaccc agcgttccgg tttgtacaca
     1441 tccagttcag taggtataac attgtaatct ccggtgagca ctaccgggat atcggaggcg
     1501 agtagcccgg ctgcgtgggc cgttaatctt tcaaaccagt tcagcttata atcgaatttc
     1561 ggtccggggg cggggtttcc gtttggaagg taaaggcagc caatcgtgat gccgttgatc
     1621 accgcctcta tataacggct atgaatatct tccggatctc cgggaagcgc ccggcggacc
     1681 tcccggattt cccggtcacg cgccaggata gcgacgccgt tccaactttt ttgtccgtgc
     1741 cagatggcgt tatagccggc gtcctgtatc gctttctccg gaaatttttc atgtggtgct
     1801 ttgagttcct ggaggcagac aacatccggc tttgtttccc cgagccagcg gaggagtacc
     1861 gccagatgcc cgttgacgcc attgacatta taggtggtaa ttttcatggt aaaatgatcg
     1921 gtgaataccc ggagatccaa tttacaaaaa atccgggcag cccttcccga tttccctctc
     1981 ttttataaat gacggacgcc ttgtgaataa aagggtttgg tttataaagc cagtctccgg
     2041 ccattgggga ggaatatccg caaatccggt atctttttta tttattctct gccttttaga
     2101 atatggttgt aatttaacag gtagccgggc gtctggtatc aggttcctgg acaggatccc
     2161 atttccatat gacagatctt caaacgagca ttcgtttaat cagcgaaatt tgtgaagagc
     2221 agccggaagc ggctgtttgg gtgaaacctg tatggagtac cgacgggtat ccggacggaa
     2281 ggaaaattgt tgattttcaa tttcactatt gtaatccggc tatcctggac ctcctcggca
     2341 aaccggcaga acagataatt ggaaaatgtg ttctggatga ccggctaccc gatccggctg
     2401 tttatcagat catttttgac cagtgccggg aggtttatga gactggcagg tctatacaat
     2461 attcttatct cagtaaggga ttgggaaaat atatcagcct tcagcgggtc agggcccatg
     2521 atggcgttct gacgacggca aggaaccgga ccggggaatt caaacttttg gaagaaaggg
     2581 aagggcaggc caggctcctg gaaaatctgg tggagcattc tccttacggc atatcccttt
     2641 atgagtccat gcgggacggg aaaggagaaa taacagactt taagttaaaa ttatgtaaca
     2701 ggaaaagctc cgaaataacg gggctgagcc tggaggaact gttcgggaaa acggttaagg
     2761 agttaatgca gctgaggggc cattccgttt actttgatat ctgtaaacgg gtcgttgaaa
     2821 agggagatcc gcattacatg gaatattatt cgacgtccag ggaccagtgg ttgggctttt
     2881 ccttcgtaaa atttggagat gggtacctgc tcaattacat cgagataacg caaacaaaga
     2941 agtacgagca ggaggcaaaa cggaatgcac aggaactcag cgctattttc aacggatcat
     3001 taaacggcat tttttccgca aaactcgtcc gcgatccgga aggtcagatc tttgacctga
     3061 tatttctgcg ggccaatgaa agtttttaca gaatgttccc ggaaacatcc ggtcagttga
     3121 ttggccagtc tctcctggcc atctcagaaa aggaggatca atcgctgttc ttaaagtatg
     3181 tggatgaagt catttcttcc ggtaaaccgg ccatccacgt attgcattat gacaattcag
     3241 gccggtggtt tgagctgtct atggcgaagc ttgaagaaga gatcatttcg gttacgctga
     3301 acgatatcac tgcacaaaaa ttggccctgc ttgaaatcga aaggcagaaa aacctgcttg
     3361 ccggtatcat gaaacagtcg cccagcgggc tggccgttat gaaagcgatc cgcgatcagg
     3421 acgggaaaat aaaagatgcg gtcccgattc tgctaaatga agcctgtgag cgcctgctgg
     3481 gtgttccgaa tgaaattttg ctggcgggga ccattggccg tttggagccg tcgctgccgg
     3541 aaagctccat tttcgatgcc gccgggcggc ttgctgccgg cgaatctatt cgcacggaat
     3601 attaccaggc aaccacctgc cggtggctcg aactcgccat cggcaaaatg gatgaagatc
     3661 attttatcaa tgtactgacc gatatcaccg agatcaagga aacacagcag cagctggagg
     3721 catccgttgc ggatctgaaa cgttccaatg agaacctgga agattttgct tatgccgctt
     3781 cccacgatct gaaagaaccg attcgtaaga tccgatacta tgccgaacag ctgaaatt
//
LOCUS       DASYCU010000385         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1098332, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000385 DASYCU010000000
VERSION     DASYCU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098332"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /gene="vgrG"
                     /locus_tag="VGM24_07785"
     CDS             <1..951
                     /gene="vgrG"
                     /locus_tag="VGM24_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEY4155312.1"
                     /translation="GHKLEQELQDWADGLLLRSRLAKIRGRAKFIGFGDIKPGDTVKI
                     SGVSDRFNGKAYVTAVRQDMGNGAWETQIQFGLDPERYAYRYRNMEDASSAGLVGAIQ
                     GLQIGVVVKLEGDPDGQDRIQVRIPVIDNNAQGTWVRIATLDAGNSRGTCFRPEIGDE
                     VVVGSINGDPRDFVMIGQLNSSNKPSPIPAKDANDEKGFTTRSKMHISFNDQTKTIKI
                     DTPAGNSITLDEQGQKIEIADQNQNKITMQSSGISLESLKNIDIKAGAVLTLSGGTSL
                     SIGAPSLSVSADADVSISGATAKLTGQGPTIISGLPVKIN"
     gene            959..1246
                     /locus_tag="VGM24_07790"
     CDS             959..1246
                     /locus_tag="VGM24_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012489250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY4155313.1"
                     /translation="MPAAARITDMHTCPLVTPPPHVGGPITGPCVPTVLIGNLPAAVI
                     GDLCVCVGPPDSIVVGSSTVIIGGRPAARQGDLTAHGGVIVGGFPTVIIGG"
     gene            1262..1690
                     /locus_tag="VGM24_07795"
     CDS             1262..1690
                     /locus_tag="VGM24_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEY4155314.1"
                     /translation="MNTSSSNSFLGTGWAFPPTFDYDTGSVEMVSAIDDINESLNILL
                     STSLGERVMQPDYGCNLNDYMFESLNNSLIGLIKHHVENSILFYEPRIIAENVDVTAA
                     DSTDLIEGKFTITVEYTIPETNSRYNYVYDYYLNEAAKPA"
     gene            1725..>4644
                     /locus_tag="VGM24_07800"
     CDS             1725..>4644
                     /locus_tag="VGM24_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155315.1"
                     /translation="MDCQCLIHPFQNDPGTSQQQRVMDALLGDAAKIDARTLADLLDY
                     FVQMSRHINYYDLQLNVNDWQPFFKSSIPFTLASIINFPLQNTQSAFDLYKSIFNKRP
                     SAAGLQLIAFSLYYRFINNINNWQLTLKDSSLPMAATVDSLIRNQLQDPVKRLIQYIN
                     AAVKNYGIQRIDFLKLSANPVWGLDQAALNATDTAYTAGTTSARRRLINLYNEFGTLT
                     TALEDAIGTLSTAAAANLEQSFIPLKEDLQKKHQPHLALLFAFLNMFRQLQNDLNGYT
                     RKHLDYFYKEMLQFRAAGAVPDKAHVVFEIQKLLKTYLIKKGLRVKDGKDNNKQDILF
                     SLDDEIVVNQTSIADKRTLFLNNLDAHAQTYVEGVYMAPVADMADGVSKPFTDDPKNF
                     STLGAKQSKYADPESGLIKPFPNARLGFILASPVLSLQAGSTRHIHIDLSCTLLDSIC
                     GDLGDFIVQANKNCCSDQSPSPVPPEPNPYPVFLRASKIYTYVKDHINFRYVYLSGDI
                     FRQAQKKGFSKDFIDSIRDKFLLVNSGLKPCYCDIPQYNTEDTVRWSVWDAFVGPTIT
                     DPGQRKLLDDLFPPQKILNLSFSGDKDWIAPPANDYTVHLDPLGAGNTFKMKIDVTLQ
                     PVTGAVVNADPLVLKEDFGTVQPLVKVQLNDKIKLKWDITPDPAATQGNNKCCEPVDS
                     CCLLFEDNPPPQIVSYYHFFRNVQISQEADTVPVIEVHVCGLKNFIVQNDDTVMDVNG
                     PVYPFGTRPEILNYDINNPSPTTLVGPGFYIGSEEIFFKKWDYVSVNVKWKDKPANFK
                     DYYAAYVDGGILESGFKMKASVLDNGKWIDIGLEKQLFQNSFIYAGGGPTAPPHQICG
                     HTNPVDNAYLVFSSSSGIDRVFITDQHLNKKLEAATLNGFLRISLLDQDFLHKIYAFV
                     LARQMMALGRLPGALLTGAVYRNNATNAVFTFADSFLALRDLVPDSASAKASADATAT
                     K"
BASE COUNT         1178 a         1252 c         1194 g         1020 t
ORIGIN      
        1 ggtcataagc tcgaacagga attgcaggat tgggcagatg gcctgctgct gcgcagccgg
       61 ctggccaaga tccggggccg tgcaaaattt attgggttcg gagatatcaa acccggagat
      121 acggtaaaga tcagcggggt aagcgaccgg ttcaacggca aggcctatgt aacagcggtt
      181 cgtcaggata tgggaaacgg cgcctgggaa acgcaaatcc agttcggact ggatcccgaa
      241 cgctatgcat accgctaccg aaatatggaa gacgcttcat ccgccgggct cgttggtgcg
      301 atacagggac tgcagatcgg cgtggtggtg aaactggaag gcgatccgga cgggcaggac
      361 aggatacagg tacggattcc cgttatcgat aataatgcgc aaggcacctg ggttcgcatc
      421 gccaccctcg atgcaggcaa tagccgcggc acctgtttcc gccccgagat cggcgatgaa
      481 gtggtggtgg gcagcatcaa tggcgatccc cgcgacttcg tgatgatcgg ccagctgaac
      541 agcagcaata aaccttcccc cattcctgcc aaagatgcca atgatgaaaa aggatttacc
      601 acgcggagta aaatgcatat cagtttcaat gatcagacga agacgataaa gatcgacaca
      661 ccggccggca acagcattac gctcgatgag cagggacaga aaatagagat cgccgatcag
      721 aaccagaaca agatcaccat gcagtcctcc ggtatttccc tggaaagcct caagaatatt
      781 gacatcaaag cgggcgcggt gctcacgctg agcggaggca cttcgctctc tatcggtgct
      841 ccttcgctgt cggtcagtgc ggatgcggat gtcagtatct ccggtgcaac agcaaaatta
      901 accgggcagg gcccgaccat tatatccgga cttccggtaa aaataaatta accagaccat
      961 gcctgcagca gcccgtataa cagacatgca tacctgcccg ctggtgacgc cgcccccgca
     1021 tgtgggcgga cccatcaccg ggccctgtgt gccaacagtg ctgatcggca acctgcctgc
     1081 agcggtgatc ggcgatctct gtgtatgtgt gggtccgccg gactctatcg tcgtcggatc
     1141 gtccacggtg atcatcggtg gcagaccggc agcccgacag ggagacctga cggcacacgg
     1201 aggcgtaatc gttggcggat ttccaacggt aatcattggc ggatgaaaaa aaattatagt
     1261 tatgaacact tcttcatcaa attcttttct cggaaccggc tgggcttttc cgccgacatt
     1321 cgactatgat accggctcgg tggaaatggt cagcgccatc gacgacatca atgaaagcct
     1381 gaatatcctg ctctctacca gcctgggaga acgggtaatg caaccggact acggctgcaa
     1441 cctcaacgac tatatgtttg aatcactcaa caatagtctg atcggactca tcaaacatca
     1501 cgtggaaaac tcgatccttt tttatgagcc gcgcatcatt gcggagaatg ttgacgtgac
     1561 ggcagcggat tccactgatc tgattgaagg gaagtttacc attacggtgg aatataccat
     1621 acccgaaacc aattccagat acaattatgt gtatgattat tatctgaacg aggcggccaa
     1681 gccggcctga cggataagat cagcagggac caatatattt caccatggat tgtcagtgcc
     1741 tcatacaccc ttttcagaat gaccccggca ccagccagca gcaacgggta atggatgcgc
     1801 tgctgggaga cgccgccaaa atcgacgcgc gcacgctcgc cgatctgctg gattattttg
     1861 tccagatgtc gcggcacatc aactattacg acctccagct caacgtaaac gactggcagc
     1921 cgttttttaa aagcagtatt ccgtttaccc tggcgtccat catcaatttt cccctgcaga
     1981 atacccagtc cgctttcgac ctgtacaaat ccattttcaa caaaaggccc tccgccgccg
     2041 gcctgcagct gattgctttt tcgctttatt accgcttcat caataacatc aacaactggc
     2101 agctgaccct gaaggacagc agcctgccca tggccgcgac ggtcgacagc ctgatccgaa
     2161 accagttgca ggatcccgtg aaacgcctga tacaatacat caatgccgcg gttaaaaact
     2221 atggaattca gcggatcgat tttttgaaat tatctgctaa ccccgtctgg ggacttgatc
     2281 aggctgcact gaatgcaacg gatacggctt atacagccgg cacgaccagc gcccgccgga
     2341 ggctgatcaa tctctataac gaatttggca ccctgaccac ggccttggag gatgccatcg
     2401 gaaccctttc aacggcagcc gccgcgaacc tggaacagag ctttattccg ttaaaggaag
     2461 acctgcagaa aaaacaccag ccgcaccttg ccctgctctt tgcctttctc aatatgttcc
     2521 ggcaactgca gaacgacctg aacggttata cccgcaagca cctcgattac ttttataagg
     2581 agatgctcca gttccgcgca gccggcgctg taccggataa ggcccatgtc gtattcgaga
     2641 ttcagaaact gctgaagacc tacctcatta aaaaaggact ccgcgttaag gacggcaagg
     2701 ataataacaa gcaggatatc ctgttttcac tggacgatga gatcgtcgtt aaccagacca
     2761 gcattgcgga taagcggacc ctcttcctga ataacctgga tgctcatgcg caaacctatg
     2821 tggaaggcgt ttacatggca ccggtagcgg atatggccga cggcgtaagc aagcccttca
     2881 cggacgaccc gaagaatttt tcaacgctcg gggcaaagca gagcaagtat gcggacccgg
     2941 aatccggtct gatcaaaccg tttccgaatg cgcgcctggg gttcatcctg gcttcgcccg
     3001 ttttgtccct gcaggccgga agcacccgtc atattcatat cgacctgtcc tgcaccctgc
     3061 tggattccat ctgcggcgac ctcggcgatt tcatcgttca ggccaacaag aactgttgca
     3121 gcgaccagag tcccagcccg gtgccgccgg aaccaaatcc ttacccggta ttccttcgcg
     3181 cttcaaagat ttatacctat gttaaggatc atatcaactt cagatatgtt tacctgagcg
     3241 gggatatatt ccggcaggca cagaaaaaag ggttcagcaa agattttatt gactcgatcc
     3301 gggataaatt cctgctggtc aattccgggc taaagccctg ctattgcgat attccgcagt
     3361 ataataccga agacacggtg cggtggtcgg tctgggatgc tttcgtcggt ccaacgatca
     3421 ccgatccggg acaacgtaag ctgctggacg acctgttccc gccccagaaa atcctgaacc
     3481 tgagcttcag cggagacaag gactggatcg cacccccggc caacgactat acggttcatc
     3541 tcgaccccct gggcgccggc aatacgttta agatgaagat tgacgtgacg ctccagccgg
     3601 tcaccggcgc cgtggtcaat gccgacccat tggtcttaaa agaagatttt ggaacggtgc
     3661 agccgctggt caaggttcag ctgaacgata agataaaact gaaatgggat ataactcccg
     3721 atccggcggc aactcaggga aataataaat gctgtgagcc ggtggacagc tgctgtttgt
     3781 tgtttgaaga taacccgcct ccccagatcg tttcctatta tcattttttc cggaacgtgc
     3841 agatcagcca ggaagcggat accgtgcccg tgattgaagt gcatgtatgc ggattgaaaa
     3901 actttattgt ccagaacgac gatacggtta tggatgtgaa cggcccggtc tatccattcg
     3961 gaacgagacc ggagattctg aactacgata taaataatcc ctccccaacc acgctcgtag
     4021 gaccgggttt ttatatcggc tcggaagaaa tattttttaa gaaatgggat tatgtcagcg
     4081 tgaatgtgaa atggaaggac aagcccgcca acttcaaaga ttattatgcc gcctatgttg
     4141 acgggggtat tctggaatcc ggctttaaaa tgaaagcctc ggtgctggac aacgggaaat
     4201 ggattgatat cggcctggaa aagcagctgt tccagaattc cttcatatat gccggcggcg
     4261 gaccgacagc cccgccgcac cagatctgcg gtcacaccaa tccggtggat aatgcatacc
     4321 tggttttctc ttccagctca gggattgacc gggtgttcat cactgatcag catctcaata
     4381 aaaagctgga ggcggccaca ctcaatggat tcttacggat cagcctcctg gatcaggatt
     4441 ttctacacaa gatttatgct tttgtgctcg cccgccagat gatggccctt ggaagactgc
     4501 cgggcgcgct cctgaccggt gcggtgtata ggaataacgc cacgaatgcg gtgtttacat
     4561 tcgccgactc attcctcgcg cttcgtgatc tggtcccgga ctcggccagt gcgaaggcca
     4621 gtgcggatgc aaccgcgacc aaga
//
LOCUS       DASYCU010000386         4530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_665467, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000386 DASYCU010000000
VERSION     DASYCU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665467"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..589)
                     /locus_tag="VGM24_07805"
     CDS             complement(266..589)
                     /locus_tag="VGM24_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155316.1"
                     /translation="MEKHATQQEILLSIDSRFAQREWAEKEINENNGLSPKDQLEKAC
                     WDGLVKELIPEVDITLMTDKKLWLWNIRRTRSFLELEFCDYPSPKDNWDSIDPYAFLE
                     TACRN"
     gene            complement(711..2015)
                     /locus_tag="VGM24_07810"
     CDS             complement(711..2015)
                     /locus_tag="VGM24_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY4155317.1"
                     /translation="MAASLLSPLQSWAQQDKLKNWSGNITYSTDKVFYPGSVKELQEW
                     VRKCRNLRSLGTRHCFNTIADSRFNLISSNKMDRVLELDPAAHTVTIEGGIKYGELSP
                     YLHQKGFALHNLASLPHISVAGSISTATHGSGVSNGNLASAVRSLEIVRADGSIVHLS
                     KDKDDLFPGAVVGLGALGFISKVGLAIEPTYQMRQNVFINLPLARLKDQFDKIMSAGY
                     SVSLFMDWQTEFINEVWIKSRVDPEKEYQPLSEFYGAAAATQNLHPIMDHSAENCTEQ
                     MGVAGPWYERLPHFKMGFTPSSGKELQSEYFVPRQHAVEAISAVYRLGKEIGSHLFIT
                     EIRTIAADELWMSPCRKQDSATIHFTWKPDWPAVSRLLPRIEKELEPFQARPHWGKLF
                     TLSPKRLQSRYEKLEDFKKLVAQYDPSGKFRNEFLNSALYAG"
     gene            2204..3229
                     /locus_tag="VGM24_07815"
     CDS             2204..3229
                     /locus_tag="VGM24_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY4155318.1"
                     /translation="MSKKWYKSGNAVIFFHILAWLLLFSLPALFHRENDQPRVNWKGM
                     FTLRALIFDACFVSLFYLNSLLLIPRLLNRKRIGAYIFSIVGLFVAVLVLLPLIPSEN
                     GPPGFHRESFFLVFPYLFIWAMSTAYRFVSNQVKTEQLLKERENENLKTELSFLRSQV
                     SPHFLFNVLNNMVAMARLKSDLLEPSLIRLSGLMRYMLYESDEAKVPLSREIEYVNSY
                     IELQKMRYARSLTIDAQLDPGDNQLIEPMLLIPFIENAFKHGTGLIEDPVIGISLRMS
                     EGLIDFRVRNKYMEDGTETKDKTSGIGLTNVIRRLNLLYDQKHILTINRENGWFTVSL
                     QIKLHNA"
     gene            3226..3930
                     /locus_tag="VGM24_07820"
     CDS             3226..3930
                     /locus_tag="VGM24_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY4155319.1"
                     /translation="MNCMAVDDEPLALALLEDNIRQVPALKRVASCSNAMEALEVLNR
                     QMVDLIFLDIQMPGLTGLQFLQSLPVKPMVILITAYEKYALEGFNLDVIDYLVKPVPF
                     ERFLKACNKAQDFFRLKNNVFLTEQPDYFFVNAEYGLIKIVISEMVFVEGLKDYLKIH
                     LADGKHLVIRMSMKTIEEKLPSNKFFRIHKSYIVALSHISSIRKNSVFLGPRELPLSD
                     HYKDDLLRVIGQGNKG"
     gene            4026..>4530
                     /locus_tag="VGM24_07825"
     CDS             4026..>4530
                     /locus_tag="VGM24_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4155320.1"
                     /translation="MKKFLLLIFSFAGLFSVRAQFAGAGGKAPNMGHIYGKIIDSTGH
                     PISEATVIVLQSKRDSATKKNKFVLLKGLSTRTNGDFNFEDLPLFGGLKLKISATGYK
                     PYEQMVVFQMRMPAGGAPKPGADPSQALSAMSNAINGFDRDLGNIKLDMDVAMLGAVT
                     VTATPPTL"
BASE COUNT         1118 a         1158 c         1079 g         1175 t
ORIGIN      
        1 aaatgatgtc caccacggcg gattttccct ttgatgcaat ctcttcagtt ttattcccca
       61 ctttagtggc cgtcgtatcc attttcttct ttacctgagc gccggcgctc agggcaagta
      121 caaagctgaa caacaatatc ccggtaattt ttttcataaa cctgttttat ctgaataacc
      181 tgattccaac aggtcttatg aaaaaacaat gccaggcagg ggagctcaac cggttcagcg
      241 gccggaatcc tgatttctgc ttccatcaat tccggcaggc ggtttcaaga aaggcatagg
      301 gatcaatgga atcccaatta tcttttggcg aaggataatc gcagaattcc agctcaagaa
      361 atgaccgggt gcgccggata ttccaaagcc atagtttttt atcagtcatc agggtgatgt
      421 cgacctccgg aatcagttcc tttaccagtc cgtcccagca ggctttttca agctggtcct
      481 ttggagaaag cccgttgttt tcattgatct ctttttcagc ccattcccgt tgggcaaacc
      541 gggaatcgat gctgaggagg atttcctgct gggtagcgtg cttttccata atctgttgtt
      601 taagcttcta cctatttgac gtaaaaaaat gcccgaggta aatcgaaacc ccgggaatct
      661 tctgtcggaa acaaataatc catctaattt atcgatccct taacaggaga tcaaccggca
      721 taaagggctg aattgagaaa ttcattcctg aatttaccgg atggatcgta ttgagcaacc
      781 agctttttaa aatcttccag tttttcatag cgggattgca atcttttcgg agaaagggta
      841 aacagttttc cccaatgcgg cctggcctga aagggttcca gttccttttc aatccggggc
      901 aataacctgc ttaccgccgg ccagtcgggt ttccaggtaa agtgaatggt tgccgaatcc
      961 tgtttccggc atggactcat ccagagctca tcggcagcaa tagtgcggat ttcggtaatg
     1021 aacaggtggg aaccaatttc ctttcccaac cggtaaacgg ctgaaatagc ttccacggca
     1081 tgttgccggg gaacaaaata ttcagactgc agttctttgc cgctgctggg cgtaaagccc
     1141 attttaaaat gcggcagccg ttcataccag ggtcccgcaa cacccatttg ctctgtacag
     1201 ttttccgcgg aatgatccat tatcgggtgc aggttttggg tggctgctgc ggccccgtaa
     1261 aattcggaaa ggggttgata ttctttttcc ggatccaccc ggctcttaat ccagacctca
     1321 ttgataaatt ccgtttgcca atccataaaa agacttacac tgtatcctgc agacatgatt
     1381 ttatcgaact gatctttcag acgggccagc gggaggttga taaaaacgtt ctggcgcatc
     1441 tggtaagtcg gctcgatggc cagtcccact ttgctgataa agcccagtgc acccagtcct
     1501 acaacggctc cgggaaacaa atcatcctta tccttcgaaa gatgaacgat actgccgtcg
     1561 gcccgcacga tttccaatga cctgacggcg gaagccaggt ttccattgct aacaccggaa
     1621 ccgtgggtgg cagtgctgat ggaacccgct acagaaatat gaggcaggga agccaggtta
     1681 tgcagcgcga accctttttg atgaagatag gggctgagct ctccatattt tattcctcct
     1741 tcaatggtaa cggtatgcgc ggcgggatcc agttccaata cccggtccat tttattggaa
     1801 gagatcaggt taaaccggct gtccgcgatc gtattaaagc aatgccgggt gcccaggctc
     1861 ctcaggttcc tgcatttcct tacccattcc tgaagctcct taacggaccc gggataaaat
     1921 accttatccg tgctgtaggt tatattaccg gaccagtttt ttaatttatc ctgctgtgcc
     1981 cagctttgaa ggggcgataa taaagaggcc gccatgccgg tcgaagttaa tttgataaat
     2041 gttttccttt ccatgccgtt gcagtgatac aatgtaatat ttattttctt aaacgggcgg
     2101 cgatccggac tttcctcccc ttatcgcaga aatcgggctc tttgtcgcag gatgccgccc
     2161 tttttttcaa tatatcctta cattggggcc gattcgattt tcaatgagta aaaaatggta
     2221 taagtcaggg aacgccgtta tctttttcca tattctggcc tggttgctcc tgttttcatt
     2281 gcctgcactc tttcataggg aaaatgacca gccccgggta aactggaaag ggatgtttac
     2341 cctgcgtgcc ctgattttcg atgcctgctt cgtcagcttg ttctacctga attccctcct
     2401 cctgatcccc cgcctgctca accgcaaacg gatcggcgcg tatattttct ccattgtggg
     2461 tttgtttgtc gccgtcctgg tcctgctgcc gctcattcct tcggaaaacg ggccacccgg
     2521 ctttcaccgg gagtccttct tcctcgtctt cccctacctc tttatctggg ccatgagcac
     2581 ggcctaccgc tttgtttcca accaggtcaa gacagagcag cttctgaaag aacgggaaaa
     2641 tgaaaacctg aaaacggaat tgtcttttct ccggtcccag gtcagtcccc attttttatt
     2701 caacgtgctg aacaatatgg tggccatggc caggttgaaa tcagacctgc tggagccttc
     2761 gctgatccgg ctatccggac tgatgcgata catgctctac gaatccgacg aagccaaagt
     2821 tcccctctcg cgggaaatcg aatacgtgaa cagctatatc gaactgcaaa aaatgcggta
     2881 cgcgagatcg ctgaccattg atgcgcagct ggacccggga gacaatcagc tcatagaacc
     2941 catgctcctg attcccttca ttgaaaatgc gttcaagcat ggtacgggac tcattgaaga
     3001 tccggtgatc ggcatttccc tccggatgag cgaggggctg attgatttcc gggtaaggaa
     3061 taaatatatg gaggacggaa cggaaacaaa agacaaaacc tccggtatcg gactgaccaa
     3121 cgtgatcagg cgcctgaacc tgctctacga tcaaaaacat attctgacca tcaaccggga
     3181 aaacggatgg ttcaccgttt ctttgcaaat aaaactgcac aacgcatgaa ctgcatggcg
     3241 gtagacgacg agcccctggc cctggccctg ctggaagaca atatccggca ggtgcccgcc
     3301 cttaaacggg tggcttcctg cagtaacgcg atggaagcgc tcgaggtgct gaaccggcag
     3361 atggtggacc tgatctttct ggatatccag atgcccggcc tcaccggcct gcagtttctt
     3421 cagtcgcttc cagtaaaacc catggtgatc ctcatcaccg cgtatgagaa atatgcattg
     3481 gaaggcttta acctggacgt aattgactac ctggtcaaac cggttccctt tgaacggttt
     3541 ttaaaggcct gcaacaaagc acaggatttc ttccggctga agaataatgt ttttttaacg
     3601 gaacagccgg attatttttt tgtgaacgcc gaatacggac tgatcaagat cgttatttcg
     3661 gaaatggtgt ttgtggaagg gctgaaagac tatttgaaga tccatctcgc ggatggcaaa
     3721 cacctggtga tccgcatgag catgaagacc attgaagaaa agcttccgtc caataaattc
     3781 ttccgtatcc ataaatccta tatcgtcgcc ctttcccata tcagttcgat ccgcaagaat
     3841 tccgttttcc tgggcccccg ggaactcccc ttaagtgacc attataaaga cgacctgctc
     3901 cgggtgatcg gacagggaaa caaggggtaa tgcatttggt cgcaggataa tgaccgcttg
     3961 tctcaaaccc gccatttaat aagaattccc gtattaggtt tgccggataa taccaaacag
     4021 attttatgaa aaagttcttg ttgctgatct tttctttcgc cggccttttt tcggtacgcg
     4081 cgcaattcgc gggtgcaggc ggtaaagcgc ctaacatggg gcatatctat ggaaaaataa
     4141 tagatagcac cggtcatccc atcagcgaag cgaccgtgat cgtactgcag agcaagcggg
     4201 actccgccac caaaaagaac aagtttgtcc tgttgaaagg gctcagcacc cggacaaacg
     4261 gggatttcaa ctttgaggac ctgccgcttt tcgggggact gaaactcaag atctctgcaa
     4321 ccggatacaa accctacgaa caaatggtgg tattccagat gagaatgccc gcgggcggtg
     4381 cgccgaagcc gggcgccgat ccgtcccagg ccctgagtgc catgagcaat gccataaacg
     4441 ggttcgaccg ggacctcgga aacataaagc tggatatgga tgtcgccatg ctgggggccg
     4501 taacggtaac cgctaccccg cccacgcttc
//
LOCUS       DASYCU010000387         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_374611, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000387 DASYCU010000000
VERSION     DASYCU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374611"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VGM24_07830"
     CDS             <1..1005
                     /locus_tag="VGM24_07830"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEY4155321.1"
                     /translation="GPRIPNTPIAMPFVHHTMPLVNMKSYVEWIHIPYTRWFAAPVKR
                     RDVVVFNFPAGDTVINKEGYESEQPYYDVARALGNGDINAGRQIILNDPDEYPLVVRP
                     VDKQENYIKRCVAVAGDTLQIKKQIVYIDGQVETLPEYSETNYIVVTRGQPLDETVMK
                     DEYDIDLGNPDEFQQTGVPNRYRILLTAGAKAKMLKNGLAKEIIPEIDSSKSVYPYDP
                     HYNWDKDDYGPIWVPKKGATIELNAMTYPLYERAIRDYEKNKLEMRDGKIFLNGKEAT
                     HYTFKMDYYWMMGDNRHMSQDSRYWGFVPEDHVVGEAWMIWMSWNKGVRWSRLFKRIR
                     "
     gene            1016..1501
                     /locus_tag="VGM24_07835"
     CDS             1016..1501
                     /locus_tag="VGM24_07835"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY4155322.1"
                     /translation="MTKKELTIPVEVYADSRELKPEDALLLDRARKATGDAYAPYSRF
                     RVAAAARLANGEILTGTNQENASFPAGICAERAALSTASAIYPGIAVTDLAVSCFQEG
                     AVNSRPISPCGICRQTLTEYELRAGRPLRLILGGYSGEVYIINCATDLLPLAFTAAEM
                     E"
     gene            1602..2084
                     /locus_tag="VGM24_07840"
     CDS             1602..2084
                     /locus_tag="VGM24_07840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY4155323.1"
                     /translation="MKKIIFSVILLSAIGYFVACNKTPQSTPSCNGVDPAQESDQLIH
                     FAADSITVTKDTTGVYFQILDSGSGVSPVGTSNLVVSYTAMRMDKTIFDSASNSNLGG
                     LQLYQLIPGWQIGLPKIKKGGHIKLLIPSAYAWGCTGSGTTIPPNSPVYFDIYLIDVI
                     "
     gene            2219..>2636
                     /locus_tag="VGM24_07845"
     CDS             2219..>2636
                     /locus_tag="VGM24_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155324.1"
                     /translation="MPKTKSSISRRNFLLNSAYTAGGIMIVPRHVLGRGFIAPSDKLN
                     IAGVGTGGKAEVNLPYAYNKGSDNIVALCDVDDRRSVNARKNWPSAPYYKDYRKMLEK
                     EAKNIDAVIVTTPDHMHAPIALAAMQLGKHVYVEKPL"
BASE COUNT          698 a          681 c          665 g          592 t
ORIGIN      
        1 ggtccccgta ttccgaatac gccgatcgcg atgccctttg tacatcatac catgccgctg
       61 gttaatatga aatcctatgt ggaatggatc catattccct atacccggtg gttcgcggca
      121 cccgttaaaa gaagggacgt ggtggtattt aattttccgg ctggcgatac ggtgatcaat
      181 aaggaaggat atgagtccga gcaaccctat tacgacgttg cccgggcttt gggaaatgga
      241 gatatcaatg cgggccgcca gatcatcctg aatgatcccg atgaatatcc gctggtcgtt
      301 cgtcccgtgg ataagcagga aaattatatc aaacgttgcg tcgccgttgc cggagacacg
      361 ctgcagatca aaaagcagat tgtttatatt gacggccagg tcgaaaccct gcccgaatat
      421 tccgaaacaa attatatcgt ggtcacccgc ggccagcccc tggatgaaac ggtcatgaaa
      481 gatgaatacg atattgacct gggtaacccg gatgaatttc agcaaaccgg cgttcccaac
      541 cgctaccgca ttctcctcac ggccggtgcc aaggcaaaaa tgctgaagaa cgggctggca
      601 aaagagatca ttccggaaat cgacagctcc aagagcgttt atccttacga tccgcactat
      661 aactgggaca aggacgacta cggtcccatc tgggttccga aaaaaggagc gactattgag
      721 ctgaacgcaa tgacctaccc gctctacgaa cgcgcgatcc gggattacga aaagaataaa
      781 ctggaaatgc gggacggaaa aatttttctg aacggaaagg aggcgacgca ctacacgttc
      841 aaaatggact actactggat gatgggcgat aaccggcata tgtcgcagga cagccgctac
      901 tggggttttg ttcctgaaga ccacgtggtc ggggaagcct ggatgatctg gatgagctgg
      961 aataagggag ttcgctggag ccggctgttc aaaaggatcc ggtgaaaata aagccatgac
     1021 aaaaaaagag ctgaccatac ccgtcgaagt atatgcagac tcccgggaac tgaaaccgga
     1081 ggacgcgctt ttgctggacc gggcccggaa agctaccggc gacgcctatg cgccgtattc
     1141 ccggttccgg gtggcggcgg cggcccggct ggccaatggg gaaatactca ccggaaccaa
     1201 tcaggaaaat gcttcctttc ccgccggcat ctgcgcggag agagcggctc tttccaccgc
     1261 atccgctatt tatcccggca tcgccgtaac ggatctcgcc gtttcctgtt tccaggaagg
     1321 ggctgtgaat tccaggccca tttctccctg tggcatatgc aggcaaaccc ttacggaata
     1381 tgaactccgg gcaggccggc cattgcgcct cattctcgga ggatattccg gcgaggtata
     1441 tatcataaat tgtgctaccg accttttacc cctggctttt actgccgcag aaatggaata
     1501 ggcccaccta ttatataaat tgttaaaatc cggtggcaag gaaacccaaa tcgttaatat
     1561 tgcgtcctgg ataataaccg gtattaaaaa ataaacctgt catgaagaaa attattttta
     1621 gcgttatcct gctttctgct atcggatatt ttgtagcctg caataaaaca cctcagtcga
     1681 ctccaagctg taacggagtg gacccggcgc aggaatctga ccagctgatt cattttgccg
     1741 ccgactcgat cacggtcacc aaggatacca ccggcgtcta ttttcaaata ctcgacagcg
     1801 gatcgggagt cagccccgtc ggcacatcaa acctcgtggt cagctatacc gcgatgcgga
     1861 tggataaaac gattttcgac agcgccagca attccaatct cggcggattg cagctgtacc
     1921 agctgattcc gggatggcag atcggattgc cgaaaattaa aaaaggcggc catatcaaac
     1981 tcctgatccc atctgcctat gcctggggtt gtacgggctc cggcacgacc attccgccaa
     2041 attctcccgt gtattttgac atttacctga tcgatgtgat ttagtttatc agtaacttac
     2101 gtttcgaaaa ttcagagaag ccaccgcggg tgccagattg aattgtccca tcagcaaagg
     2161 gttgaaatgc aaaatcacac ccggtgcatc cctggtttga ttctttaaaa acgtaggcat
     2221 gccaaagacc aaatcctcca tttcgagaag gaactttctc cttaattctg cttatactgc
     2281 cggtgggatc atgatcgttc cccgtcacgt gctcggccga gggttcatcg cacccagcga
     2341 caagctcaat attgccggag tcggcaccgg cggtaaagcc gaagtcaacc tgccctatgc
     2401 atataataag ggctctgaca atatcgtggc cctttgcgat gtggatgacc ggcggtcggt
     2461 taacgccagg aaaaactggc ccagtgcgcc ttattataag gactaccgga aaatgctgga
     2521 aaaagaagca aaaaatattg atgccgtgat cgtgaccact ccggatcaca tgcacgcacc
     2581 cattgcgctc gccgctatgc aactgggcaa acacgtatac gtggaaaaac cgctga
//
LOCUS       DASYCU010000388         8520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3998443, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000388 DASYCU010000000
VERSION     DASYCU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3998443"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="VGM24_07850"
     CDS             complement(<1..1813)
                     /locus_tag="VGM24_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4155325.1"
                     /translation="MPANCKNQSFRIPYCRLPATSLRLLLFSFIFLFSATLFAQQPIT
                     GTVRDQLNPLKGVTVQVKGKTTSTQTDDNGAFTIVASPNSKLVLTNIGYTTQEIAVDN
                     RTSINVVMTATSRQLDQVVVTGYTRQAKKDITGSVAVVDMKALKSVPAGSAEQALQGQ
                     ASGVVVINSGAPGGSSNIFIRGVSSFGDTQPLVIVDGVQSSLHDINSNDIESIQVLKD
                     AGAAAIYGVRGSNGVIIVTTKKGRPGTPTVSYDFYYGTQVPPKGNVLNIASPAAYASF
                     VKRMDPTSQLFPDGTLPDFMYGGPGVKGIGNAGDPAVDPSKYVFDAANPNNDYMIQQV
                     NKQGTDWFHQIFKSAPTQSHNLTLSGGTDKSSYLFSVGYLNQQGTLLDTYLKRYSVKV
                     NTEFKIKNTVRVGENIYAFYKQNPGFSNQDQDNAIFFAYTMPNFIPVYDINGQYGGTW
                     AGPGELGDRWNPVAMLKNTSGNKNNAWDVVGNMYGDVDILKHLTARTSFGGTIDNEYS
                     YNFMPNRYQDLEQHTGTNMYNENALYNSSWIWTNTLVYSQIFGRHNVKMLLGSEAINN
                     YGRGVGGGANGFFSSDPNYLVLGNGTSNVSNYSNAYTN"
     gene            complement(2053..3378)
                     /locus_tag="VGM24_07855"
     CDS             complement(2053..3378)
                     /locus_tag="VGM24_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY4155326.1"
                     /translation="MIIAKVPDQFSEIIEEANHYIAGGVVSLNRKVNPNIVFRKGKGS
                     KIYDTAGKEYIDYHAGFAPYLLGHNFDKVNEAVYEVIRSEWSLIGSGTNELEIKLARL
                     LCESIPSLDTVQILNTGSEGTAHAIRLARAWTGREDIIVTMGGYNGWHNDVARVVLPD
                     LQQAGPRVTKGQYPFIPSSSGIPESTKEKVHVVNFNDLDSVEYVLKNYPIACLLTEPV
                     LQNIGVVLPKEGYLQGLIDLCEKYGTVCIFDEVKTGFRSALGGYQSIAGVKPHLSVFG
                     KAVANGYPIGVIGGRRDIMELFDSPDPDKRVLLAGTYNAHPVNTAASIATIEILKNPQ
                     VYHHIEKVSNLLYKGLNALFYEKGIPFNLSANASAFCIYFCEKTPADLHDILENHDFA
                     YDTAYRKALIESGIYHIPIPCKQGSISYSHTEEDISRTLAITRKVLRSI"
     gene            3561..4592
                     /locus_tag="VGM24_07860"
     CDS             3561..4592
                     /locus_tag="VGM24_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4155327.1"
                     /translation="MKRSSIFDLIQVNEHSPTPKYLQLTKSILNAIDAGKIEKGALLP
                     SINELSFELDISRRTAEKGYAYLKHIGVLGSVPGKAFFIKRLESRQNRRIVLLFNKLS
                     VYKKMVYDSFVNCLGDNASIVLYIYNNDYLLFRKLLLEKKEEYSYYVIIPFFQNGGQD
                     ACEIINAIPKEKLILLDRQMEGVTGCYGAAWENFENDIYCAMHSARERLAKYDTIKII
                     FPNNSYYSRQILKGLSRFCSDFGFRYKAIENIQEEPIRPGEVYINLMDDDLVLLIERL
                     RDLHFIIGQDVGIISYNETPLKRIILNGITTISTDFHQMGRMAAKLIMNRSREQLAVP
                     FHLNLRESL"
     gene            4808..6472
                     /locus_tag="VGM24_07865"
     CDS             4808..6472
                     /locus_tag="VGM24_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155328.1"
                     /translation="MNKTIIVSLLATLIFEGCSSPSPRYTGLSEDIIQAKRDSVLPDS
                     IIYHPVHVDQDGNILPWYSDTLGKSYDHMLALVWGFWKNMATDSNGEKYYMNHQVWRP
                     NHDKRGIGGDQVMMALSSWDLYYDYTGDESLLENMRYMADYYLAHSLSSPDCQWPDLP
                     YPYNDAVETGIYDGDMILGKDYLQPDKAGSFGFELVHLYKKTGDQKYLNAAIKIANTL
                     ASKVQPGDSDHSPWPFKVNAKTGEPGVIVDKVVWYEGMSKDIKAKKQKSKESVYTSNW
                     TGTLGLFSELNALHAGNAEAYKKAFDIALGWMKNYPAKTNKWGPFFEDVPRWSDTQIN
                     GITYAMYLLEHKELDPDWKATVAHIIQWVHNELGDKQYLKYGVICTDEQTAYRVPGQS
                     HSSREASVELMYWEKTGDTLYARNAVRELSWATYMVDNDGKNFYPTNDIWMTDGYGDY
                     VRHYLRAMAAAPQLAPDNADHMLRSSSIVKNISYRPEGISYSVFDSASEESFRLQSRP
                     GNITLNGNALKEIQDSSSEGWLWQDMDKGGLLKIRHQHGNKIEISR"
     gene            complement(6504..7931)
                     /locus_tag="VGM24_07870"
     CDS             complement(6504..7931)
                     /locus_tag="VGM24_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY4155329.1"
                     /translation="MNRKSGSPISILSAELKKTAAAFLPALLLCLMSLPASAARGPVS
                     GNPPPPRSAAGLQQLLDKAKPGDTVIIPEGIYKLNDRPIRPHVSGNAHAWITIMPGKG
                     KQVIIDGSNYLSRYSGGKQPLSALVLFKNCRYIRMKGIEVRNSHDAGIVMQGPNTDHI
                     EIIRCKSDGSYNSGISMWYADSCRILQCEVVAANNKDLKPAGAPFRGEAPHEAISLAG
                     ATHFEVAYNRVHDCFKEGIDCKEVSRNGIIHHNVVYNMPRQGLYTDCWFGRLSGIRFY
                     DNTVYNCEWGFAISGEGRNASMDSIYFHHNLLYNNRASGIFISVWGFDEPRKDIFIYN
                     NTVYHNGTAGHWSGLTGGIDIMSSHTRNLYIYNNIISDNYGYAVGLWAPDAAKDSLLK
                     NRNIRILDNLEWQQNKPGTKNPKAIFKPMYPIELSSDIKADPRFVDAAAGNFRLSASS
                     PARKKGWSAAPTGKADFLGALAPAK"
     gene            8215..>8520
                     /locus_tag="VGM24_07875"
     CDS             8215..>8520
                     /locus_tag="VGM24_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155330.1"
                     /translation="MTTATAAEKRISSRPEITASLPANRIASVDVFRGFVMLLMMAEV
                     LSFERVSNALPGNIFWGILSFNQTHVEWTWLGLHDMIQPSFTFLVGVVLPFSMESRKK
                     "
BASE COUNT         2097 a         2116 c         2195 g         2112 t
ORIGIN      
        1 tattcgtata ggcattactg taattgctta cgttggacgt gccgttgccc agcacgaggt
       61 agttgggatc gctggagaag aagccgttgg ctccgccgcc cacgcctctg ccatagttgt
      121 tgatggcttc ggaacccagc agcattttta cattgtgccg gccgaagatc tggctgtaga
      181 ccagggtatt ggtccatatc cagctgctgt tgtacagcgc attctcattg tacatattgg
      241 tgccggtatg ctgctccagg tcctggtagc ggttcggcat gaaattataa ctgtactcgt
      301 tgtcgatcgt accgccgaag ctggtccttg ccgtaaggtg tttcaggata tccacgtcac
      361 catacatatt cccaaccaca tcccaggcat tgttcttgtt gccggaagta tttttcagca
      421 tggccaccgg gttccagcgg tcacccagtt ctccgggtcc ggcccaggtt ccgccgtact
      481 gcccgttgat atcgtaaacc gggatgaagt tgggcatggt ataagcgaag aagatcgcat
      541 tgtcctggtc ctggttactg aaccccgggt tctgcttgta aaaggcatag atattctcgc
      601 cgacgcggac cgtgttcttg atcttaaatt cggtgttcac ctttacggaa tatcttttta
      661 aataggtatc cagcagggtt ccctgctgat tcagatatcc cactgaaaaa agataactcg
      721 atttatccgt accgccgctc agggtcaggt tatgactctg ggtgggagcg gatttaaaga
      781 tctggtgaaa ccagtcggtg ccttgtttat tgacctgctg gatcatatag tcgttgttcg
      841 ggttggcggc gtcaaaaaca tattttgagg ggtcgaccgc aggatctccc gcattgccaa
      901 tccccttaac acccggtccg ccgtacatga aatccggaag ggtgccgtcg ggaaagagct
      961 ggcttgtagg atccattctt tttacgaagc tggcataggc cgccggactg gcaatgttta
     1021 aaacatttcc tttgggagga acctgggttc cgtaataaaa atcgtaagaa acggtcggcg
     1081 tgccgggcct tcctttttta gtggtgacga tgatcacgcc gttggatccg cgcaccccgt
     1141 agatggctgc ggcgccggca tctttcaata cctggatgga ttcgatatcg ttggagttta
     1201 tatcgtgcag gctgctttgc accccgtcca cgatcaccag cggctgggta tccccgaaag
     1261 aggaaacgcc ccggataaag atattgctgc tgcctccggg cgcaccggaa ttgatcacca
     1321 ccacgccgga agcctggccc tggagcgcct gttcggcaga tccggcaggg accgacttca
     1381 acgctttcat gtccaccacc gcaacggatc cggtgatatc tttcttggcc tgccgggtgt
     1441 aaccggtcac cactacctgg tcgagctggc gggaagtggc cgtcatgacc acgttgatgg
     1501 aagtccggtt atccaccgca atttcctgcg tggtatagcc gatattggtg agaacgagct
     1561 tcgaattagg cgaagcaaca atagtaaagg cgccgttgtc atcggtttgc gtgctggtgg
     1621 ttttcccctt tacctggacg gtcacccctt ttaagggatt caactggtca cggaccgttc
     1681 cggtgatggg ttgctgggca aacagggtag ctgaaaaaag aaaaataaaa gaaaaaagaa
     1741 ggagccggag gctggttgcc gggagccggc agtacggaat tctaaatgac tggttcttgc
     1801 aatttgctgg cataatcgtt cagttttggg aataaaattt agtttggagt tcccggaaga
     1861 gataattgtt tacaaaagca cttatgtttt gtaaacataa aaaattgttt taaataaaca
     1921 attcttttta accaaaaatc tttgggagcc tccggccgtt gctgcaaatc cgggacaggg
     1981 gctgcgtgga aaagggaaac acgaagtttc tcttcccggc aacaataggg cagttgatcc
     2041 tgacctgcgg gatcagatcg atctcagcac cttccgagtg atcgccaggg tgcggctgat
     2101 atcctcctct gtatgggaat agctgatgga accctgcttg caggggatcg ggatatgata
     2161 aattccggat tcgatcagcg ctttccgata ggcggtgtca taggcgaaat cgtgattctc
     2221 cagtatgtcg tggaggtccg cgggtgtttt ttcacagaaa taaatacaga aggcggaagc
     2281 gttggcggag agattaaagg gaatgccctt ttcataaaac agcgcattca atcctttata
     2341 cagcaggttg ctcacttttt cgatatggtg gtagacctgc gggttcttca atatttcaat
     2401 cgtggcaatg gacgccgcgg tattcaccgg gtgggcattg taggtgccgg ccagcagaac
     2461 gcgtttatcg gggtcgggcg aatcaaagag ctccatgata tcccggcgcc cgccgatgac
     2521 gccgatgggg tatccgttag ccaccgcttt tccgaatacc gatagatgtg gtttcacccc
     2581 ggcaatgctc tggtagccgc ccagggcaga ccggaagccg gtcttcacct catcgaaaat
     2641 gcagacggtc ccgtattttt cgcagaggtc gatcagcccc tgcaggtatc cttcttttgg
     2701 caggaccacc ccgatattct ggagcacggg ttccgttaag aggcaggcaa tgggatagtt
     2761 ttttaataca tactcgacgg agtccaggtc attgaaattg accacatgca ctttttcctt
     2821 ggtcgattcc ggtatgccgg aagaggaggg aataaagggg tactggcctt tggtcacccg
     2881 gggcccggcc tgttgcaggt cgggcaggac caccctggcc acatcgttat gccagccgtt
     2941 gtatccgccc atagtaacta tgatatcttc gcgccccgtc caggccctgg ccaggcggat
     3001 ggcatgggcc gttccttcac tgcccgtatt gaggatctgg accgtatcca gggaggggat
     3061 ggattcgcac agcagcctgg ccagtttaat ctccagctcg ttggtgccgc ttccgatcag
     3121 ggaccattcc gaccggatca cctcataaac cgcctcgttc accttgtcaa aattgtgtcc
     3181 taacagatag ggggcaaagc cggcatggta gtctatatat tcttttcccg cggtatcgta
     3241 gatcttgctg ccctttcctt tcctgaatac aatgttcgga ttcaccttcc ggttaagcga
     3301 aacaacgccg cccgctatat agtggttggc ttcctcgatg atttctgaaa attgatccgg
     3361 gaccttggcg atgatcatat gtcttgattt aaggattggt tggcggttac ccggatttta
     3421 aggaaaatcc ggccgtttat tcgtgaatgt atacaaaaaa gcattataat tgtacacaaa
     3481 tataaatatt ccggttgatt ttttacacac aatttcatga ggagacagat taaagcacct
     3541 gatttattaa tacaatcact atgaaaaggt cttccatatt cgatctgatc caggtgaatg
     3601 aacattcccc cacgccgaaa tatttacagc tcacgaaatc cattttaaat gccattgacg
     3661 caggaaagat tgagaaaggc gccctgcttc cttccatcaa tgaactgagc ttcgaactgg
     3721 acatttccag gagaacggcg gaaaagggat atgcctatct caagcatatc ggcgtgctgg
     3781 gctccgttcc cgggaaggct ttttttatca agcggctgga gtcccggcag aaccggcgga
     3841 tcgttctgct gttcaataaa ttgagcgtgt ataagaaaat ggtttacgat tctttcgtga
     3901 actgcctggg ggataatgcc agcatcgtcc tctatatata taataacgac tacctcctgt
     3961 tccggaaatt attgctggag aaaaaagaag agtacagcta ttacgtgatc attccttttt
     4021 ttcagaacgg cggacaggat gcctgtgaga tcatcaatgc gattcccaaa gaaaagctga
     4081 tcctgctgga ccggcagatg gagggcgtta ccggatgcta tggggccgcc tgggaaaatt
     4141 tcgaaaacga tatctattgc gccatgcaca gtgcgcggga aagactggcc aaatacgata
     4201 ccatcaagat cattttcccg aataacagtt actattcaag acagatcctc aaaggacttt
     4261 cccggttctg ctccgatttt ggtttccggt ataaggcgat tgaaaatatc caggaggaac
     4321 cgatccgtcc cggagaggtt tatatcaacc tgatggatga cgacctggtg ctcctgatcg
     4381 aacggttgag ggacctccat tttatcatcg ggcaggacgt gggcataatt tcttataatg
     4441 aaacgccttt aaagcggatc atcctgaacg gcatcacgac gatctccaca gactttcacc
     4501 agatgggcag gatggcggcc aagctgatca tgaaccgttc gcgggaacag cttgccgtgc
     4561 cctttcatct taacctgcgg gaatctttat gagtgcagag agaagttttt gagaacgcag
     4621 ctcttttctc atccggcaga ggatggtgga taacggctga acccgggaaa cgacttagtt
     4681 tagcgctgtt taggcagttt tgcagtatct atggtttaat aacgcgggag cagtgtctat
     4741 tgctcccgct tattttattt ctttaccggt ttttttataa aacagttcac ccatcaagtt
     4801 tctgaaaatg aataaaacga taatcgtcag cctcctggcc acgctgatct tcgaaggatg
     4861 cagcagcccg tctccccggt atacgggcct gagcgaagat atcattcagg ctaaaagaga
     4921 cagcgttctt ccggacagta tcatctatca cccggtgcac gttgaccagg acgggaatat
     4981 ccttccctgg tattccgata ccctgggaaa atcctacgac cacatgctgg ccctggtctg
     5041 ggggttctgg aaaaatatgg cgaccgattc caacggagaa aagtattata tgaatcacca
     5101 ggtctggaga cccaatcacg acaagcgcgg gatcggcggc gaccaggtaa tgatggcgct
     5161 ttcttcctgg gacctctact atgattatac cggcgacgaa tcgctcctgg aaaatatgcg
     5221 gtatatggcc gattattacc tggcccattc cctgtcatcg cccgactgcc aatggcccga
     5281 cctgccctat ccctataacg atgcggtgga aaccggcatt tatgacggcg atatgatcct
     5341 ggggaaagat tacctgcagc ccgacaaagc agggtctttc ggattcgagc tggtgcacct
     5401 ttacaaaaag accggggatc agaaatattt aaatgctgca ataaagatcg ccaataccct
     5461 ggcctctaaa gtacaacccg gcgacagcga ccattctccc tggcccttca aggtgaatgc
     5521 gaaaaccggc gaacccggcg ttatcgtgga taaagtggtc tggtatgaag gcatgtcgaa
     5581 ggacatcaaa gcaaaaaaac agaagagcaa ggaatccgtt tacaccagca actggaccgg
     5641 caccctgggc ttattttcag agctcaatgc gctgcatgcc ggcaatgcgg aagcgtataa
     5701 aaaggcattc gatatagcgc tcggctggat gaaaaattac cctgccaaaa caaataaatg
     5761 gggacccttc ttcgaagacg taccccgctg gtcggatacc cagatcaacg gaatcaccta
     5821 tgccatgtac ctgctggaac ataaggaact ggatccggat tggaaagcaa cggtcgctca
     5881 tattatccag tgggtgcaca atgaactggg agataagcaa tacctcaaat acggcgtgat
     5941 ctgcaccgac gagcagacgg cctaccgtgt accgggccag agccattctt cgcgcgaagc
     6001 ttccgttgaa ctgatgtact gggaaaagac cggcgatacc ctttacgcgc ggaacgccgt
     6061 tcgcgaactg agctgggcaa cctatatggt ggacaatgac gggaaaaact tttacccgac
     6121 caacgatatc tggatgaccg acggctatgg cgattatgtc cggcattatc tccgggccat
     6181 ggccgccgct ccgcaactgg cgccggacaa tgcagaccat atgcttcgca gctcttccat
     6241 cgtgaaaaat atttcttacc gcccggaagg gatctcctac tcggtattcg acagcgcatc
     6301 cgaagaaagc ttccgtcttc aatccaggcc gggaaacatc acgctcaacg gaaatgccct
     6361 gaaggaaatt caggacagca gctccgaagg ttggttatgg caggatatgg acaaaggagg
     6421 gctgttgaag atccgccacc agcacggaaa caaaattgaa atttccaggt aagcggaaaa
     6481 tcctccccgc cggtcccggc aatttattta gccggggcca gcgctcccag aaaatcagcc
     6541 ttcccggtcg gtgcggcgga ccatcctttt ttcctcgccg gagaagaggc cgacaaacgg
     6601 aaattgccgg cggccgcatc cacaaaacgg ggatccgctt ttatatcgga tgacagttcg
     6661 atcggataca tcggtttgaa aatggccttc gggtttttcg tccccggttt attctgctgc
     6721 cattccaggt tgtcgaggat gcggatattc cggtttttca acaggctgtc ctttgccgca
     6781 tccggagccc agagcccgac ggcatatccg taattgtccg aaataatatt attatagata
     6841 tacaggttcc gggtatggga ggacatgatg tcgattcccc cggtgagccc ggaccagtgg
     6901 ccggccgtgc cgttatggta taccgtattg ttgtaaataa aaatatcctt tcgcggttca
     6961 tcgaagcccc agacgctgat aaaaattccg gatgcccggt tattgtacaa aaggttgtga
     7021 tgaaaataaa tgctgtccat gctggcgttc cgtccttccc cgctgatcgc aaaaccccat
     7081 tcgcaattgt ataccgtgtt atcgtaaaac cggatcccgc ttaagcggcc gaaccagcag
     7141 tccgtgtaca atccctgtct cggcatattg tacacgacat tgtgatgaat gatcccgttc
     7201 cggctcactt ccttgcaatc gatgccttct ttaaagcagt cgtgaaccct gttgtacgcc
     7261 acttcaaaat gggtggcgcc ggccaggctg atggcttcat gcggcgcttc tccccggaac
     7321 ggggcgcctg ccggtttcag gtctttgtta ttggcggcaa ccacttcaca ctgcaggatc
     7381 cggcaggagt ccgcatacca catcgaaatc cccgaattat aggagccgtc cgatttgcaa
     7441 cggatgatct cgatatgatc cgtgttcggg ccctgcatca cgatgccggc atcgtgcgaa
     7501 ttcctcactt caatcccttt catccggata tagcggcagt tcttaaagag gaccaaagcg
     7561 gaaagcggtt gtttgccccc ggagtacctg cttaaataat tgctcccgtc aatgatcacc
     7621 tgttttcctt tccccggcat gatcgtgatc caggcatggg catttcccga aacatgcggc
     7681 cggatgggac ggtcattcaa cttatatatt ccttcgggaa taataaccgt atcgccgggt
     7741 tttgctttat ccaggagttg ctgaagtccg gctgcgcttc tcgggggagg aggattcccg
     7801 gagaccggtc cccgggcagc cgatgcggga agggacatca ggcaaagcaa tagggcagga
     7861 agaaaagcgg cggcggtttt ttttagctcc gcagaaagaa tggagatcgg tgatccggat
     7921 ttgcgattca ttgtagaatg tataaaagat aaaatttgaa tgaataaaaa gataaacctt
     7981 aattggataa aaagatgaac gataaaataa aaacggtctt tccaatgtct aaagttcaaa
     8041 aaatacagac aggaaatccg gtcctgcttt ttattgttca taaataaaaa agactgaaga
     8101 ggatgccgtc ctgtcaaaaa aagatagtgc aggacggtaa atccttattt taaacatatc
     8161 ttaataccca ggttgaaatc cgctccgtca aaaaaataaa acccattcat gccaatgact
     8221 actgcaacag ctgctgaaaa gcgtatttct tccaggccgg agatcaccgc ctccctgccg
     8281 gccaaccgga tcgcctcggt ggatgtattc cgcggcttcg tgatgttgct gatgatggcg
     8341 gaagtgcttt cctttgagcg cgtgtcgaac gcgctgcccg gaaatatttt ctgggggatc
     8401 ctgtcattta accagaccca tgtggaatgg acctggctgg gcctgcacga tatgatccag
     8461 ccttccttta cgtttttagt cggcgtggtg ctgcccttct cgatggagag ccgcaagaag
//
LOCUS       DASYCU010000389         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1826803, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000389 DASYCU010000000
VERSION     DASYCU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826803"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VGM24_07880"
     CDS             <1..1524
                     /locus_tag="VGM24_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY4155331.1"
                     /translation="IGFSYLMERGFREDIIRKFQLGYDPASADAFASHALSSQYSPEI
                     LQKSGLVVWRDGKAFDNYRGRIIFPIHSQTGKITGFGARIIRNNDRAPKYINTPENEI
                     YIKSKILYGSYFARQAIDKLDECLLVEGYTDVISLHQAGVENVVASGGTSLTPDQLRL
                     IKKYTSNLTILYDGDAAGIKAAQRGMDLAFEESLNVSLVLIPDQEDPDSYVRKKGAAA
                     FRDLISANKKDFILFQLETSLSAVGNDSNKKAVLVNQIADTISRINKAEDFTKQQDYI
                     RRSSELLKIDENGLNSLVNKFIRERMQKWENRAAGQQKQEEILPDTPESDETLNDLLN
                     RDEQQERAIVRSLLEFGLKLWDEERRVADYMLPEFEDEDMIDNKNLVHILRIYKTWYE
                     QGLEPTARNFLYQEDQQLSTLVVSIMDFPYEVSENWKTFYDGKIATREELYKEEVRST
                     LTYLKLRKIRRLMVMNQKDLERSQSADEQVMYMQTHLHLKQMERELMDNSGTVIVKI"
BASE COUNT          460 a          393 c          423 g          342 t
ORIGIN      
        1 atcggctttt cctatctgat ggaacgcggg ttccgggagg atataatccg gaaattccag
       61 ctggggtatg atcccgcttc ggcagatgct ttcgccagcc atgccctctc ttcgcagtac
      121 agcccggaga tccttcaaaa aagcgggctg gttgtttggc gcgatggaaa agcatttgat
      181 aattaccggg gccggatcat ttttcccatt cacagtcaga ccggaaaaat taccggattc
      241 ggagcaagga ttatccgaaa caatgaccgc gccccgaaat acatcaatac cccggaaaat
      301 gagatctata taaaaagcaa gattctgtat ggttcttatt ttgcccggca ggcgattgac
      361 aagctggatg aatgtttact cgtggaaggg tataccgatg tgatatccct ccaccaggcc
      421 ggcgttgaaa acgtagtggc cagcggcggc acctcactca ctccggacca gctccggctc
      481 atcaaaaaat atacatccaa cctgaccatt ttatatgatg gcgacgctgc cggaataaaa
      541 gcggcgcaac ggggcatgga cctggccttc gaagaaagcc tgaacgtaag cctggtgctg
      601 atccccgacc aggaagatcc ggatagctat gtccggaaaa aaggcgcagc tgcctttcgc
      661 gatctcatct cggcaaacaa aaaggatttt attctttttc agctggagac ctctctatct
      721 gccgttggga acgacagcaa caaaaaagca gtcctggtaa accagatcgc ggataccatt
      781 tcacgcatca acaaagccga agattttaca aagcagcagg attatatccg ccgttcatcc
      841 gaattgttga aaattgacga gaacgggctc aatagcctgg tgaataaatt catccgggag
      901 cggatgcaga aatgggagaa ccgggcggcc ggccagcaaa agcaggagga aattcttccg
      961 gacacgcccg aatccgatga aacgcttaat gacctgctga acagggacga acagcaggaa
     1021 cgggccatcg tccgttcgct gctggaattc ggattgaaat tatgggatga agaaagacgg
     1081 gtggcagact atatgcttcc cgaatttgag gatgaggaca tgatagacaa taagaacctg
     1141 gtccatatcc tccgcattta taagacctgg tatgagcagg gactggaacc caccgcgcgc
     1201 aatttccttt accaggagga tcagcaactg agcaccctgg tggtctctat catggacttc
     1261 ccctatgaag tcagtgagaa ctggaaaacg ttttacgatg ggaagattgc cacccgggaa
     1321 gagctttata aagaggaagt gcggtctacc ttaacctacc tcaagctccg gaaaatccgc
     1381 cggctgatgg ttatgaatca aaaagatctc gagcgctcac aaagcgcgga tgaacaggta
     1441 atgtacatgc aaacgcatct tcacctgaaa cagatggaaa gggaactgat ggataactcg
     1501 ggaacggtga tcgttaaaat ctgaaccggg attaggggga atgaccggtc aacgggcaac
     1561 catcgccgta aggccgcggt tgctcaggac accctgacgg ctggcttttt caaagcgg
//
LOCUS       DASYCU010000390         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2550025, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000390 DASYCU010000000
VERSION     DASYCU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550025"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VGM24_07885"
     CDS             <1..1070
                     /locus_tag="VGM24_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY4155332.1"
                     /translation="PAGHEGKGKVFGYQYELDPSSRRWTAGIYDEGRRDWLYPLSLHP
                     SAQNAFKLNEFNKVRIECVGNNLRTWINGIPAAYVVDSMDHKGFIALQVHAIQKPEFA
                     GEKIYWKNIRIRPISTLTDDYPKDAYVQNNIPNHLSPAEKKNGWKLLFDGKTSTGWRG
                     AFKTGFPEKGWRIKDGEITVLSSAGKESANGGDIVTLKEYKAFDISFEFKLTPGANSG
                     LKYFVTLKEDSKGYAIGLEYQVLDDKLHPDAKLGRNGDRTLASLYDLIPAAKQARFVH
                     PIGEWNTGRVVVFPNNHVIHYLNGVKVLEYDRGSPAFRQLVAISKFKVWPHFGEGAEG
                     HILLQDHGFEVSFRSIKIREL"
     gene            1089..2405
                     /locus_tag="VGM24_07890"
     CDS             1089..2405
                     /locus_tag="VGM24_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155333.1"
                     /translation="MERRDFIKNASVAGAGLTVLNFPVFGKKAPSNKVVLAVMGLNGR
                     GSYLAKSYSSLNNVEIAYICDVEDGAIQHGFDALKAAPRKPVLIRDIRELVNKKDFDA
                     LLVAAPDHWHTPAAILGVSHGKHVYVEKPCGQNPAEGELIADALHHYGKLIQMGNQRR
                     SFPTLIQAAKEVRDGVIGDVYFARAWYSNNRKSIGTGKKIPVPPNLNFELWQGPAPRR
                     DYQSNLVHYNWHWFWHWGTGEACNNGTHEIDCCRWFLGVDFPTKVTSSGGRYAFHDDW
                     QTPDTQVAAFEFGNEKCIMWESRSCNLFPVDGADRGFNIYGKKGTLVNLAGGDYKIYD
                     ADNKLVKEVKSEVVADPTNPISATGNLDLYHFQNFVDSIRGEAHLNSPVDEVQKSVLL
                     CHLANIAQRTGRALHCDPKNGHILGDPGAMELWKREYEKGWEPKIG"
     gene            2479..>3280
                     /locus_tag="VGM24_07895"
     CDS             2479..>3280
                     /locus_tag="VGM24_07895"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEY4155334.1"
                     /translation="MIRYFFGLMALLIVQSGSAQKFEQLAMTPPMGWNSWNKFACDGV
                     NEQVIRQTADAMVSSGMKDAGYEYIVIDDCWQIDRDSSGNIVADPKKFPSGIKALADY
                     VHSKGLKFGIYSCAGRKTCAGRPGSRGHEYQDAISYARWGVDYLKLDWCNAQGQNAKD
                     SYTLMRDALYKAGRPIVFSLCEWGSNKPWEWAGDIGELWRSSGDIANSWGKMEGNDGK
                     IWGGSVLANLDLQRGLEKYAGPGRWNDPDMLEVGNGALTPAEDRAHFSL"
BASE COUNT          856 a          836 c          912 g          676 t
ORIGIN      
        1 acccggccgg tcacgagggc aaggggaaag tttttggcta ccagtatgaa ctggatcctt
       61 cttcccgccg gtggaccgcc ggcatttacg atgagggccg ccgcgactgg ctttatccgc
      121 tttccctgca tccctccgcc cagaatgcct ttaaactgaa tgaattcaat aaggtgagaa
      181 ttgagtgcgt cggcaataac ctccgcacct ggatcaacgg aattccggca gcctacgtgg
      241 tggacagcat ggatcataaa ggattcattg ccctgcaggt gcatgccatc cagaaacctg
      301 aattcgccgg ggaaaagatt tactggaaga atatacgcat ccggccgatc agcacgctga
      361 ctgatgatta cccgaaagat gcctatgtac agaacaatat tcccaatcat ttatcaccgg
      421 cggagaaaaa gaacggatgg aaattattat ttgacggaaa aacctccacc ggttggcgcg
      481 gagcttttaa aacaggcttc ccggaaaaag gctggagaat aaaagacgga gagatcaccg
      541 tgctctcttc cgcagggaaa gaatccgcca acggaggcga tatcgttacg ctgaaggaat
      601 acaaagcctt tgatatttcc ttcgaattca aactcacccc gggcgccaac agcgggctga
      661 aatattttgt cacgctgaaa gaagacagca aaggctacgc catcggactg gaataccagg
      721 tgctcgatga taaattacat cccgatgcca agctcgggcg gaacggagac cgcaccctgg
      781 cctctttgta cgatctcatc ccggcggcca aacaggcccg ctttgtgcat ccgatcggtg
      841 aatggaatac cggccgggtg gtcgtttttc caaataatca cgtgatccat tatctcaacg
      901 gggtcaaggt gctggaatac gatcgcggtt cccccgcttt ccgccagctc gtggcgatca
      961 gtaagtttaa ggtctggccg catttcggcg aaggcgccga aggccatatc ctcctgcagg
     1021 atcacgggtt tgaagtgagt ttccggagca tcaagatccg ggaactgtaa tcctcaaaaa
     1081 aagcaaaaat ggaaagaaga gattttataa agaatgcctc ggtggccggc gccggactca
     1141 ccgtcctgaa ttttccggtc ttcgggaaaa aagcgccctc caataaagtg gtgcttgccg
     1201 taatgggttt gaacgggcgg ggttcctatc tcgccaaatc gtattcctcc ctgaacaatg
     1261 tcgagatcgc atatatctgc gacgtggaag acggcgccat ccagcatggc ttcgacgcgc
     1321 tgaaagccgc gccgcgcaaa ccggtgctga tccgcgatat acgcgaactg gtcaataaaa
     1381 aagattttga cgccctgctc gttgctgcgc cggaccactg gcatacgccg gccgccatac
     1441 tgggggtaag tcatggaaaa catgtgtatg ttgaaaaacc ctgcggacaa aatccggcgg
     1501 aaggagagct gattgccgat gcgctgcacc attatggcaa actgatccag atgggaaacc
     1561 agcgcagatc ctttcccacg ctcattcagg ccgcgaaaga agtgcgggac ggagtcatcg
     1621 gcgacgttta cttcgccagg gcctggtact ccaataaccg gaaatccatc gggaccggga
     1681 aaaagatccc ggtccctccc aatctgaatt ttgaactctg gcagggtcca gccccccgca
     1741 gggattatca aagcaacctc gttcattata actggcactg gttctggcat tggggaaccg
     1801 gggaagcctg taataacgga acgcatgaga tcgattgctg ccggtggttc ctgggcgtgg
     1861 attttccaac gaaagtcact tcctccggcg gccgttatgc gtttcacgac gactggcaga
     1921 cgccggatac acaggttgcg gcctttgaat tcggaaatga aaaatgcatt atgtgggaaa
     1981 gcaggagctg taatttgttc ccggtcgacg gggcggaccg ggggtttaac atctacggta
     2041 aaaagggaac cctggtcaat ctggctggcg gcgattacaa aatatatgat gcggacaata
     2101 aacttgtcaa agaggtaaaa tcggaggtgg ttgccgatcc caccaatccc atcagcgcta
     2161 cgggcaacct ggacctctat cattttcaga atttcgtcga cagcatccgc ggggaagccc
     2221 atttgaattc cccggtggat gaagtgcaaa aaagcgtttt gctttgtcat ctcgccaata
     2281 ttgcccagcg taccggccgg gccctgcact gcgatccgaa aaacggccat atcctgggcg
     2341 atcccggagc catggaactc tggaaacggg aatatgaaaa aggctgggaa ccgaagatcg
     2401 gctgataaaa aaagtaaacc cattcatcat tggcgctggt aaaaatcgtt gagcgccgga
     2461 gaaaataaaa caaaagatat gatcaggtat tttttcggac tgatggccct gctgatcgtt
     2521 cagtccgggt ccgcacaaaa attcgaacaa ctggcgatga ccccgcccat gggatggaac
     2581 agctggaata aatttgcctg cgatggcgtg aatgagcagg tgatccgtca aaccgccgac
     2641 gccatggttt cgagcgggat gaaagatgcc ggctatgaat atatcgtgat tgacgattgc
     2701 tggcagatcg accgcgacag ttccgggaat atcgtagccg atcctaaaaa atttccctcc
     2761 ggcatcaagg ccctcgcgga ttacgtgcac agcaagggat tgaaattcgg catctattcc
     2821 tgtgcgggaa gaaagacctg cgccggccgc ccgggcagcc ggggccacga ataccaggat
     2881 gccatcagtt atgcccgctg gggagtggat tacctgaaac tggactggtg caatgcccag
     2941 ggccagaacg caaaagattc ctacaccctc atgcgggacg ctctctacaa agctggccgg
     3001 cccatcgtat tcagcctttg cgaatgggga agcaacaaac cctgggaatg ggccggggac
     3061 atcggggaat tatggagaag ctcgggcgat attgccaata gctggggtaa aatggagggt
     3121 aacgacggaa agatctgggg cggaagcgtg ctggccaacc tggacctgca aagagggttg
     3181 gaaaaatatg ccgggccggg acggtggaat gatccggaca tgctggaagt agggaacggt
     3241 gcgctgactc ctgccgaaga ccgcgctcat ttcagtctct
//
LOCUS       DASYCU010000391         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2404431, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000391 DASYCU010000000
VERSION     DASYCU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404431"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VGM24_07900"
     CDS             complement(<1..935)
                     /locus_tag="VGM24_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEY4155335.1"
                     /translation="MSQIISIGTAVPRFKHDQQEILRFMSRIYAFNEKDRRKLKYLYA
                     QSGISTRYSVLPDYSQLPEDWTFYPPGENLDPFPSLEKRMNIFNLHAPALSAEAIRNC
                     LRDQSFPGEITHLITVTCTGLSAPGLDLQVMELLDLPKNIFRTSVNFMGCYAAIHAMK
                     LADAICTAEPDARVMIVCTELCTLHFQQEPTLDNITSSLLFGDGSAAMLMTGTADKTP
                     GLKIRGFYAEVNAMGKQDMSWSLSSTGFAMTLSSYVPGLIEEDFRHMLCHALSRNQLT
                     VDAITDWCIHPGGRKILEAIEKSLHLPKEKLSAGS"
     gene            complement(956..1828)
                     /locus_tag="VGM24_07905"
     CDS             complement(956..1828)
                     /locus_tag="VGM24_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY4155336.1"
                     /translation="MTIYRSTIQLLRFHFSFFLMPVYWFALSQLPDPDPGAAMLIFFI
                     LHGLLYPSSNGYNSYMDRDEGSIGGLQSPPAPTRQLYRVTLLMDLLALALSLCISGWF
                     MLGSFLYILASRAYSARQIRLKRYPVTGYLTVIIFQGALVFFMVSHGADPALPLRIPV
                     AGMVAAALLVGGAYPLTQIYQHEADARDGVRTLSALLGYRGTFIFSALLYGLALGTLA
                     ILFLLGLELKEFMVFATLMVPVIVYFTVWAVQVWRDPAKADFHHSMRMNGIASICTNL
                     GFIIVFLMHRYHYH"
     gene            2020..>2795
                     /locus_tag="VGM24_07910"
     CDS             2020..>2795
                     /locus_tag="VGM24_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155337.1"
                     /translation="MKTKMCNRICGLLLTGILFPFLMQAQVAQWNFNNTLNGTGSANA
                     TVKPALLGSAITQGDYNGGTVYFGEGGWPAGALDPNAYLEFSVSPTSSYSLTIASMVM
                     SIRRSTTGTSGSGPNSWALRSSLDGYAADISHGTLSTSVLAIPVTFDASYANLSGTVT
                     FRLYGYNATVSANGGLNRFVYDNILISGSTILPVVFESFKASKNNQAAELSWTLGGEG
                     TIAQITVERAINGNAFETITEMAQQTDLNYSYKDVQIQTSG"
BASE COUNT          691 a          707 c          745 g          652 t
ORIGIN      
        1 tcagacccgg ccgacagttt ttctttgggc agatggagac ttttttcaat ggcttcgaga
       61 atttttctgc ctccgggatg aatgcaccag tccgtaatgg cgtcaacggt cagctggttc
      121 cggctgaggg catggcacag catatggcgg aaatcttctt cgatcaggcc gggcacatag
      181 ctgctgagcg tcatggcgaa accggtggag gacagcgacc agctcatatc ctgttttccc
      241 atcgcattga cttcagcgta aaaaccgcgg atctttaatc cgggcgtctt gtcggccgtt
      301 ccggtcatca gcatggccgc cgatccgtcg ccaaacagca ggctcgacgt gatgttgtcc
      361 agggttggct cctgttgaaa atgcaaggtg cacaactctg tgcaaacgat catcacacgc
      421 gcgtccggtt cggcggtaca aatggcatcg gccagcttca tcgcatgaat ggccgcataa
      481 catcccataa agttcaccga ggtccgaaaa atattttttg gcaggtccag caattccatc
      541 acctggagat ccaggcccgg agcgcttaaa ccggtgcagg tgacggtgat gagatgggta
      601 atttctccgg gaaaagactg gtctcgaaga cagttgcgga tggcttcggc ggatagtgcc
      661 ggagcatgga gattgaatat gttcatcctt ttttccaggg aagggaaggg gtcgagattc
      721 tcccccggtg gataaaaggt ccagtcttcc ggcaactggc tgtagtccgg taaaacggag
      781 taccgggtgg aaattccgct ttgggcatag aggtatttca gtttccgccg gtccttttca
      841 ttaaatgcgt atatccggct catgaaacgc aggatttcct gctggtcatg cttaaaccgg
      901 ggaactgccg tgccgatgga gatgatctga ctcaaaagtt tgttttaaga cgacgttaat
      961 gatagtggta gcgatgcatc agaaatacaa ttataaagcc aaggttggta cagatggagg
     1021 cgatgccgtt catgcgcatg ctgtgatgga aatcggcttt cgcgggatct cgccatacct
     1081 gtactgccca cacggtaaaa tatacgataa ccggaaccat cagggttgca aacaccataa
     1141 attcttttaa ctccaggccg agcaggaaaa ggatggccaa agttcccaga gccaatccat
     1201 acagcagggc ggaaaaaata aaagttcccc ggtagcccag cagggcactc agggtcctga
     1261 cgccatcccg ggcatccgcc tcatgctggt atatctgcgt cagcgggtag gccccgccca
     1321 cgaggagggc agcggctacc ataccggcca cgggtatacg gagcgggagc gcaggatccg
     1381 cgccgtgcga aaccataaaa aataccagcg ccccctgaaa aataatgacg gtcaggtaac
     1441 cggtaaccgg atagcgcttt agcctgatct ggcgggcact gtaggccctg gaggcaagga
     1501 tgtataaaaa actacccagc ataaaccagc cgctgatgca aagactcagt gccagggcca
     1561 gcaaatccat caggagggta acccggtaca gctgccgggt gggggcgggc ggcgattgca
     1621 ggcctccgat gctgccttca tcccggtcca tataactgtt atagccgttg ctggatgggt
     1681 ataataatcc gtggaggatg aaaaaaatca gcatggcggc cccgggatcc gggtcgggaa
     1741 gctggctcag ggcaaaccaa tagaccggca tcagaaaaaa cgaaaaatgg aagcgcagca
     1801 gttgaatggt ggaccggtaa atagtcataa aaagggcaaa ctgtaattta tcattctttt
     1861 gcgtcaatac cgttccggac ggcctttcgg gccattttct ccggtcggcg gaatacgttc
     1921 ccgtcccggg aataaaattg atttttaacc tattgaaaat cagccgaatt gcccttggta
     1981 tgaaattcgt ctgttagaca tcgatttcaa atccgaacta tgaaaaccaa gatgtgtaac
     2041 agaatttgcg gactcctttt aaccggaatc ttatttcctt tcctgatgca ggcacaggtt
     2101 gcccagtgga atttcaacaa taccctgaat ggaacaggat ctgccaatgc caccgtaaaa
     2161 ccggcgctcc tgggatctgc catcacgcag ggtgattata atggaggtac cgtttatttc
     2221 ggcgaaggcg gttggccggc cggtgccctc gatccgaatg cctatctcga attttcagtt
     2281 tctccgacca gctcctacag tctgaccatt gcttctatgg tgatgtctat ccgcaggagc
     2341 accacgggta cctcgggatc cggtcccaac agctgggctt tacgttccag tcttgatgga
     2401 tacgccgccg acatttccca cggaaccttg tccaccagcg tactggctat tccggttact
     2461 ttcgatgctt cttatgccaa tctttccgga acggtcactt tccgtttgta tggttataat
     2521 gccacggttt ctgccaatgg cggtctgaac cggtttgttt acgataatat tttgatctcc
     2581 ggttccacga tcctgcccgt tgtttttgaa tccttcaagg cttcaaaaaa caatcaggcc
     2641 gccgagctga gctggacgct cggtggcgaa ggaaccatcg cgcagatcac cgtagaacgg
     2701 gccatcaacg gtaatgcctt tgaaacgatc acggaaatgg cgcagcaaac agatttgaat
     2761 tattcttata aagatgtgca gatccagact tccgg
//
LOCUS       DASYCU010000392         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2118733, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000392 DASYCU010000000
VERSION     DASYCU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118733"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VGM24_07915"
     CDS             <1..591
                     /locus_tag="VGM24_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY4155338.1"
                     /translation="NLGIGIPTLVANYIPAGMGIMIQSENGILGMGAYPLESELDPDL
                     INAGKETVTLLPGGAFFDSAESFGMIRAGKVDLTVLGAMEVSEKGDIANWKIPGKMVK
                     GMGGAMDLVSSARNIIVAMQHTNPKGQSKLLPECSLPLTGVRCVKKIVTDLAVMAVTP
                     DGFLLLERAPGVSVEEIQAKTLGRLIVKGKIPEMQS"
     gene            621..1547
                     /locus_tag="VGM24_07920"
     CDS             621..1547
                     /locus_tag="VGM24_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155339.1"
                     /translation="MEGFIKNVGPLEIVTLENGFIRCEMAPAAGGRITSVYNHAMRKE
                     FLWSNQDLPLEKPEPGTDYDTHFWGGIDELIPNDIPETIDSVLYPDHGELWTTALSQE
                     GSEKKVMLHGKLPLSGLYYEKTLSLDASSAVIHLDYRIVNVSGSERNFLWKLHAALAI
                     QEGDRLITGARRARIADLEYSRFTQPGEFAWPFIEHTDASVIPARNQTMDFFYLYDIR
                     QGEMRLENDSDGSVFACRYDKEVFPYQWYFASYGKFFGHYMAILEPCSSMPMSVNDAK
                     RNGNCSRLEPRQEINTRVSIYAGEKNNYANNG"
BASE COUNT          407 a          370 c          457 g          355 t
ORIGIN      
        1 aacctgggca tcggcattcc caccctggtg gccaattata ttcctgccgg catgggcatc
       61 atgatccagt cggagaacgg catactgggc atgggagctt atccgctgga aagcgaactg
      121 gatccggacc tcatcaatgc cggaaaagaa acggtaaccc tcctgcccgg aggcgccttt
      181 ttcgacagtg ccgaaagttt cggcatgatc cgcgcaggga aagtggacct gacggtgctg
      241 ggcgcgatgg aagtgtcgga gaagggtgat atcgccaact ggaagattcc cgggaaaatg
      301 gtgaagggca tggggggcgc catggacctg gtatccagtg cgcggaacat tatcgtggcc
      361 atgcaacata ccaatcccaa aggccagtcc aagctgcttc ccgaatgcag cctgccgctt
      421 accggcgtcc ggtgcgtaaa aaagatcgtg accgatctgg cggtgatggc ggtaactccg
      481 gatggttttt tattgctgga gcgcgcaccg ggtgtttctg ttgaagaaat acaggccaaa
      541 accctgggcc ggttgatcgt gaaggggaaa attccggaaa tgcaaagctg acgcatggcg
      601 gccactaaaa tagctgaacg atggagggtt ttataaaaaa tgtcggacca cttgagattg
      661 taaccctgga gaacggattt atccgctgtg aaatggcgcc cgcggccgga ggccggatca
      721 ccagcgtgta taatcatgcg atgagaaagg aattcctttg gtctaaccag gacctgcctt
      781 tggaaaaacc ggaacccggg accgactatg atacccattt ctggggaggg atcgatgaac
      841 tgattccgaa tgatattccg gaaaccatcg attccgttct gtatccggat cacggggagt
      901 tatggaccac ggctttatcg caggagggat ctgaaaaaaa agtaatgctg cacggaaaat
      961 taccgctcag cggattgtat tatgaaaaaa ccctttcgct ggatgccagc tcagcggtca
     1021 ttcatctgga ttaccggatc gtcaacgtgt ccggttcgga aaggaatttt ttgtggaaat
     1081 tgcatgcggc actggccata caggaaggag acaggctcat tacgggcgca cgcagggccc
     1141 ggattgcaga cctggaatat tcccggttta cgcagccggg agaatttgcg tggcctttca
     1201 ttgagcatac ggatgcttcg gttatacctg cccgtaacca gaccatggat ttcttttatc
     1261 tctacgatat ccggcaggga gaaatgcgcc tggagaatga ttcggacgga tccgtatttg
     1321 cctgccgtta tgataaagag gtattcccgt atcaatggta ttttgcctct tatggcaaat
     1381 tcttcggtca ttatatggcc atactggagc cctgcagcag tatgcccatg agcgtgaatg
     1441 atgcgaagcg caacggaaat tgcagccggc tcgaaccgcg acaggaaatc aatacccggg
     1501 tcagtatcta tgcgggtgaa aaaaataatt atgcaaacaa cggatagaaa aagagttttg
     1561 attaccggtg cggcttccgg gattggaag
//
LOCUS       DASYCU010000393         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1244528, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000393 DASYCU010000000
VERSION     DASYCU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244528"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1161
                     /locus_tag="VGM24_07925"
     CDS             4..1161
                     /locus_tag="VGM24_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155340.1"
                     /translation="MISFSHMLNDTIQSLIPSIYPVVKKSFHLNFSEVGLITLTFQMA
                     ASLLQPMVGTYTDRKPKPFSLVTGMGFTLIGLLLLSRADSFTVLLVSVGLIGVGSSIF
                     HPEASKVAFMASGGKRGLAQSIFQVGGNAGSSLGPLLAAMIIVPFGQRSIGWFSLLAL
                     LAMMVLIRVGLWYKTNHQQIRGKSKMISEGHHLSRKKVILSIGILLVLIFSKYFYLAS
                     MTSYFTFYLINKFQVSVQHSQIYLFIFLLSVAAGTILGGPLGDRFGRKYVIWFSILGV
                     APFTLLLPHANLFWTGILTVFIGIILSSAFPAILVYAQELLPGKIGMISGLFFGFAFG
                     MGGLGSALLGSLADRTSIIFVYQVCAFLPLIGLLTGFLPDIGGKKNRTQTG"
     gene            complement(1203..2339)
                     /locus_tag="VGM24_07930"
     CDS             complement(1203..2339)
                     /locus_tag="VGM24_07930"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HEY4155341.1"
                     /translation="MKAIHWLMLLMVLGCKDKYNAPVHIPATGYLVVEGFINVGSGST
                     DFLLTRATGLDSPYTRTEPGALVSVESENGESYPLAEKGEGKYSISQVPVDFNQRYKV
                     RVRTTDGKEYESDLTSPRLTPPIDSVSWKPADGGVWIYVTTHDPAGNSRYYQWNYEET
                     WEYESAYLSGQEYLGSGRLQPRPDADLIHICYIGDLSNSISIGSTAKLESDLMYEYPL
                     VFVPYGTTNKLGRKYSILVKQYVLTKEWYEWKEKVKRNTEQLGSIFDAQPSDISGNLH
                     CISNPGEAVIGYVGCSTETEKRIFISHSELPQTRIYTGYEGCVIDTIANDPDDLERYF
                     DGGNTIPIAAYYVNGFLTDYLGSSSYCVDCRVMGGVLEKPPFWQ"
     gene            complement(2449..>2902)
                     /locus_tag="VGM24_07935"
     CDS             complement(2449..>2902)
                     /locus_tag="VGM24_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155342.1"
                     /translation="PLILLDGVPIFDADKLFQQDPLKIRRLDLISSEYFLGYHFFDGV
                     INCTTYHGDLDGFEMDPHATVLDYPGIPEQREFFSPQYETEQQAASRLPDFRTLLYWT
                     PQIETDGQGKGKVRFYTSDLPGRYAVVVQGITGTGASGSRIIYFNVKK"
BASE COUNT          637 a          765 c          698 g          802 t
ORIGIN      
        1 ctgatgatca gtttttcgca catgctcaat gatacgatcc agtcgctgat cccttccatc
       61 tacccggtcg taaagaagtc ttttcacctt aatttctccg aggttggcct gatcaccctt
      121 acttttcaga tggccgcctc cctgctgcag ccgatggtgg gcacctatac cgaccggaaa
      181 ccaaaaccct tttccctggt tacggggatg ggctttaccc tgatcgggct cctgctttta
      241 tcccgcgcag acagttttac cgttttgtta gtctcggttg gattgatcgg cgtgggttct
      301 tccatattcc atccggaagc ttcgaaagtt gcctttatgg catccggcgg taaacggggt
      361 ctggcccaat cgatatttca ggtaggcgga aatgcaggca gttcgctggg tccgctgctg
      421 gccgcgatga tcatcgttcc cttcgggcag cgtagcatcg gctggttttc cctcctggcc
      481 ctactggcca tgatggtact gatccgggtt ggtctatggt ataaaacaaa tcaccagcag
      541 atcaggggaa aatcaaaaat gatttctgaa gggcatcatt tatccagaaa gaaggtcatt
      601 ctttccattg gtattttact ggtgctcatt ttctccaaat atttttacct ggcttcaatg
      661 accagctatt ttacctttta cctgatcaat aaattccagg tctccgttca gcattcacag
      721 atttacctgt tcatcttttt actttccgtg gcagcgggga cgatcctcgg tggtccgttg
      781 ggcgaccggt tcggaagaaa gtatgtgatc tggttttcga tcctcggagt ggctccgttc
      841 acgctgctgc ttccccatgc caatcttttc tggaccggca ttctcaccgt tttcatcgga
      901 ataatccttt cctctgcctt tccggccata ctcgtatatg cgcaggaatt gctgcccgga
      961 aaaatcggta tgatctccgg tctttttttt ggatttgctt ttggaatggg cggccttggt
     1021 tctgcattac tgggttctct cgcagaccgg accagcatta tttttgttta ccaggtatgt
     1081 gctttccttc ccctgatcgg tttgctgacg gggttcctgc ccgatatcgg cgggaaaaaa
     1141 aacaggactc agacgggctg aacggatcag ggaatccagg tcaatgagat ccgggtgcag
     1201 acctattgcc agaaaggcgg tttttccagg actccgccca taacccggca gtcaacgcag
     1261 taactgctgg agcccagata atcggtcagg aacccattca cataataagc ggcgatggga
     1321 atggtgtttc cgccatcaaa atacctttcc aggtcatcgg gatcattcgc aatcgtatct
     1381 attacgcaac cttcataacc cgtataaata cgggtttggg gaagctcgga atggctgatg
     1441 aaaattcgtt tctccgtttc ggtggaacaa ccgacgtatc cgatcaccgc ttcgccgggg
     1501 ttggatatac agtgaaggtt cccggatatg tccgagggct gggcgtcaaa aatggatccc
     1561 agttgctccg tatttctttt caccttttcc ttccattcgt accattcttt tgtcagcaca
     1621 tattgtttta cgagtataga gtattttctc cccagcttat tggttgttcc gtagggaaca
     1681 aagaccagcg ggtattcata catcaggtcc gattccagct tggccgtgga accaatggaa
     1741 atactgttcg ataaatcgcc gatatagcag atatggatca ggtcagcatc cggtctgggc
     1801 tgtaaccggc cggaacccag gtattcctgc ccggaaagat aggccgattc gtattcccag
     1861 gtttcttcgt aattccattg ataataccgg gagtttcctg ccgggtcgtg cgtggtcaca
     1921 tatatccaga cgccgccgtc cgccggcttc cagctcacgc tgtcgatggg gggagtgagc
     1981 cggggagaag taagatccga ttcatactcc ttgccgtccg tagtccggac ccgcactttg
     2041 taccgctgat tgaaatccac cggcacctgg ctgatggaat attttccctc tcccttttcg
     2101 gccagcggat agctttctcc gttttccgat tccacgctca ccagggcgcc cggctccgtg
     2161 cgggtatacg gagagtccag gcccgtggcc cgggtcagca ggaaatcggt cgaaccactt
     2221 cccacattga taaagccttc cacgaccagg tagcccgttg ccgggatatg aacgggggcg
     2281 ttatatttat ccttacaacc cagcaccatc aacagcatca accaatgaat cgctttcata
     2341 aacccttttt aaaaccttcc attatccgtt ccggtactga atacctttta gaaaacggaa
     2401 ggtcgccatt ccgtgtttgt ttttgaaaaa ataataccgg atccattttt attttttaac
     2461 gttgaaataa atgatccggc ttcccgaagc gccggtccct gtaattccct gtacgaccac
     2521 cgcatatctt ccgggcaggt cagaggtata aaagcgcacc ttcccttttc cctggccatc
     2581 ggtttctatc tgtggcgtcc agtaaagcag cgttctgaaa tccggcaacc ggctggcggc
     2641 ctgctgctct gtttcatatt gcggcgaaaa gaattcccgt tgttcaggaa tgcccggata
     2701 atcaagtacc gtggcgtggg gatccatctc aaacccatcg agatctccat gataggtggt
     2761 gcagttgatc acgccgtcaa aaaaatgata tcccaggaaa tattcactgg agatcagatc
     2821 caggcggcgg atcttgagcg ggtcctgttg aaataacttg tctgcatcaa aaatgggcac
     2881 cccatccaat aagatcagcg gc
//
LOCUS       DASYCU010000394         9980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4144661, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000394 DASYCU010000000
VERSION     DASYCU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9980
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4144661"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VGM24_07940"
     CDS             <1..251
                     /locus_tag="VGM24_07940"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY4155343.1"
                     /translation="NLNVGYHNEHHDFPSVPWNKLPRIRNIAGNYYNSLGFHTSYTIL
                     LFKFLTDRNLSVYSRMARDNRGKKKTKSAQQPISVDGI"
     gene            complement(234..773)
                     /gene="idi"
                     /locus_tag="VGM24_07945"
     CDS             complement(234..773)
                     /gene="idi"
                     /locus_tag="VGM24_07945"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEY4155344.1"
                     /translation="MEEEVILVNEQDEQTGTIEKMEAHRKALLHRAFSIFIFNGKGEM
                     LLQQRAAAKYHSPGLWTNACCSHPRPGEPVDRAAQRRLKEEMGFETELKKIFDFIYKV
                     NFDNGLTEFEFDHVYAGHYGGRGLNPDKQEVLDYCFRSVEDIQHDLDSCPEKYSTWFR
                     IAFPRVKDWLESDPSYSIH"
     gene            complement(846..1349)
                     /locus_tag="VGM24_07950"
     CDS             complement(846..1349)
                     /locus_tag="VGM24_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY4155345.1"
                     /translation="MGALEFNQLLVSNADFLKPFAITLTRDSEAAKDLFQETLYRAIA
                     NKEKYNVGTNIKAWLYTIMRNIFINNYRRKSKQKTIFDSSPNDYLLNNSQSAVISDTA
                     ESNLRIRDIQGAIHNLPDIFRNPFLLYFDGYKYHEIADMLQEPLGTIKSRIHFARKLL
                     KAQIQMF"
     gene            complement(1513..2253)
                     /locus_tag="VGM24_07955"
     CDS             complement(1513..2253)
                     /locus_tag="VGM24_07955"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEY4155346.1"
                     /translation="MLRLFRKKDDSPVDFSGLVSDMHSHLIPGIDDGATDLESSIVMI
                     RAMYELGYRKLISTPHVQWEMYRNTSDIINRGAQQVRDRLEGTEMKIVFRGAAEYYLD
                     EHIDELLEKDEPLLTISGNLVLVEFSFIRQPLDLRDKLFQLQIKGYQPIIAHPERYLY
                     FGVHKELYDELHDMGCVFQLNLLSLCGYYGKKQLELANYLIKKKYVSLLGSDLHNFRH
                     INILRASSSINSTVNRLLDAGALLNPDL"
     gene            complement(2307..3242)
                     /locus_tag="VGM24_07960"
     CDS             complement(2307..3242)
                     /locus_tag="VGM24_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEY4155347.1"
                     /translation="MNKQEKIYVAGHRGMVGSAILRKLEAEGFNNLIYRTSKELDLKD
                     PAAVRAFFEENQPAYVFLAAAKVGGILANDRYRADFLYDNLMIQNNVIHSAWKSGVKK
                     LMFLGSSCIYPKLAPQPLKEEYLLTGLLEHTNEPYAIAKIAGIKLCDAFRAQYGCNFI
                     SVMPTNLYGPNDHYDLQNSHVLPALIRKFHEAKINGEPFVSIWGTGEPKREFLHVEDL
                     ADACLFLMQEYNDSGLLNIGTGKDLSIMELAGLIGKITGYEGSIRRDLSKPDGTPRKL
                     LDVSKLEALGWKARISLEEGIQRVYAAYGKERAAV"
     gene            complement(3311..4426)
                     /gene="gmd"
                     /locus_tag="VGM24_07965"
     CDS             complement(3311..4426)
                     /gene="gmd"
                     /locus_tag="VGM24_07965"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY4155348.1"
                     /translation="MAKVALITGVTGQDGAYLSSFLLDKGYEVHGIKRRSSLFNTDRI
                     DHLYQDPHEKHIHFRLHYGDLTDSTNLIRIIQETQPDEIYNLGAMSHVQVSFETPEYT
                     ANADGIGMLRILEAVRLLGLVKKTKIYQASTSELYGLAQAVPQSETTPFYPRSPYAVA
                     KLYAYWIAVNYREAYQMFAANGILFNHESPMRGETFVTRKITRAAAKIGMGLQDKLYL
                     GNLNARRDWGHAKDYVEAMWLILQQEKAEDFVIATGVTTTVRDFVKMAFAELGIQLVF
                     KGKDDREYAEVVSSTNPDYPVETGKKVVVVDPGYFRPTEVDLLIGDARKAKEKLGWEP
                     KYDLAALVREMIHADVEIFRREKILKESGFEIKNQYE"
     gene            complement(4433..5188)
                     /locus_tag="VGM24_07970"
     CDS             complement(4433..5188)
                     /locus_tag="VGM24_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4155349.1"
                     /translation="MSPLSVSIITATYNSASTVEETMRSVSGQDYPFIEHIIVDGQSQ
                     DETLKIVRGFSHTALVVCEKDTGIYDAMNKGIRASRGDLVGILNSDDVYAHSGVISRI
                     VEAFQDEKMQACYADLQYTHAQDTSRITRTWKSGHFQPGSFYWGWMPPHPTFFVRKSV
                     YDRVGLFDTSLRSASDYEMMLRILLKYKMPAGYIPEVIVKMRTGGQSNASVRNRLRAN
                     QEDRMAWRLNGLHPYFFTLYLKPLRKIAQFIIR"
     gene            complement(5204..6934)
                     /locus_tag="VGM24_07975"
     CDS             complement(5204..6934)
                     /locus_tag="VGM24_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4155350.1"
                     /translation="MFILGINAYHADSSAAIFKDGELVAATEEERFRRVKHWAGFPSQ
                     AVEFCLKEAGIRLHEVDHIAIGRDPYAKLARKITYLLKNPGGGFRAVKDRISNAKKVA
                     SLESEFKSLDPSLDEKAFKSKIHHVEHHRSHLASAFFASPFEESAILSIDGSGDFTTT
                     MTATGKGNKIAVLDSVDFPHSAGLFYTAFTQLLGFPHYGDEYKVMGLAPYGEPKYVDR
                     LKEVLLFDSNGLFRLNLKYFRSAKSGIISYGEDHVPVVAPLYSEYMTGKFGEPRQPGE
                     PLTAYHKDLGASVQRITELLIFHILESLQKKTGLKNICIAGGVAQNSVANGKLTRNTA
                     FENIYIPSAGHDAGISMGAALYVYNQVLGQPRQKAVYSAFTGSRFSNEEIETYLQSRN
                     ISYKRYPEDELYDIVTDRLIRSGVVGWFNGRAEFGPRALGARSIIADPRRSDAKDLLN
                     SKIKRRESFRPFAPSILKEYVSEYFDVTDEVPFMEKVFPIKSTKHAILPAVTHVDGTG
                     RLQSVDKQVAPRYYRLIETFRQKTGVPVLLNTSFNENEPIVNSPEHALECFLRTDMDM
                     LVLENCVITR"
     gene            complement(6937..8085)
                     /locus_tag="VGM24_07980"
     CDS             complement(6937..8085)
                     /locus_tag="VGM24_07980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4155351.1"
                     /translation="MPPGNILILTDWYEPGYKAGGPIQSCRNFVSAMHESYRLSVLTL
                     DRDLGDTENYPGIQPGEWTHREPGLPVYYATAETLNRRLLADLIEFRNPDFIYLNSMY
                     SYRFSILPLFLLWRKKIRAQIILAPRGMLQQGALKFKPGKKKLFIALLNVLGISRKIH
                     FHATDEQEKRDILRQFPKAGRVEVISNFSGSVPAYSVPVKKMAGELRCVYISRIIPKK
                     NILFFLKMLNLVPERIQLQFTIYGEVEDEKYWLRAQKVIHFLPGNIEVLYRGPLPHAE
                     VIPAMEANHVFVLPTLGENFGHAVFEALSAGRPVLISDQTPWRGLRDEQTGWDISLDN
                     LEAWLAALLEAADADQQAFDAWIEKCRQFAKNRQDASNLKNEYIKLFS"
     gene            complement(8093..8929)
                     /locus_tag="VGM24_07985"
     CDS             complement(8093..8929)
                     /locus_tag="VGM24_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4155352.1"
                     /translation="MIDSLRASGQKLQCSYCGSTDCKPIDRKYVVTCLMECQNCHLYF
                     RYPVDKKETNAAFYQTEYKEKDKITTDLPDKPMLDRMIREGFQTANKNGDRYLNLFAR
                     LFPGVKSLNIIDYGCSWGYLTYQFKQAGHRVQGYEISEPRADYGRKNLDVEIFSDENR
                     MSGSNHIFFSSHVIEHHPDIRGMIRLAKSLLINGGYFIAFCPNGSPAYRERFPQAFHR
                     AWGKVHPNYLSAGFYEHVFKDNPCYIGSSPIKPDKIGPLEKQEIQVDDLSGEELFVIS
                     RL"
     gene            complement(9002..>9980)
                     /locus_tag="VGM24_07990"
     CDS             complement(9002..>9980)
                     /locus_tag="VGM24_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4155353.1"
                     /translation="EYDESEYAGLIAKKFNTRHVQIRLKPEVMLTELTNALDAMDVPS
                     GDGINTYVVSKAVHREGIRVALSGTGGDELFAGYPIFEHYIRFQQKRWLWKAPAIVKN
                     SLAGLLGKGAKRERMKQFLALPSPAIEYTYPVLRQLLSPRALGEWTRLKGPARHELAQ
                     QLISKREALVRLPFFSQVTAAEYLGYTQQTLLKDMDQMSMANSLEIREPFFDQDLVEF
                     VMSVPDHFKVPVYPKSLLVESLKPLLPDEIVHRKKQGFVFPWNEWIKKELRSFCEKHI
                     NRMAQRDFIQEEHLKSAWKQFLSGHPDIRWQEIWLFVVLDYWMEKNGCE"
BASE COUNT         2419 a         2558 c         2396 g         2607 t
ORIGIN      
        1 taaacctcaa cgtgggttat cacaatgaac accacgattt tccttccgtt ccctggaaca
       61 aattaccccg tatccggaac attgccggca attactataa ttccctgggc tttcacactt
      121 cttataccat cctgcttttt aaattcctga ccgaccggaa tctttctgtg tattcacgca
      181 tggcgcgcga caaccgggga aagaagaaga cgaagagtgc ccagcaaccc atttcagtgg
      241 atggaatatg acggatcgct ttccagccaa tctttcactc tgggaaaagc gatcctgaac
      301 caggtactgt atttttcggg acaggaatcc agatcatgtt gaatatcttc cactgaacgg
      361 aagcaataat ccagcacctc ctgcttgtcg ggattcaggc cacggcctcc gtaatgaccg
      421 gcatagacat gatcgaattc aaattcggtg aggccgttgt caaaattcac tttataaata
      481 aaatcgaaga tttttttgag ctccgtttca aaacccattt cctcctttag ccggcgttgg
      541 gctgcccgat ccaccggttc cccgggacgc ggatggctgc agcaggcgtt ggtccacaaa
      601 cccgggctgt gatatttagc ggcggcccgc tgctgcaaca gcatctcccc tttaccattg
      661 aatataaaaa tgctgaaagc cctgtgtaaa agggctttcc ggtgagcttc cattttttct
      721 atggttcccg tctgttcatc ctgttcattg accaggatca cttcttcctc catttaaata
      781 cgttgagatt tccgggttga aagttagggt ttccttttta ttgactgtcc attctcaact
      841 cccattcaaa acatttgtat ctgcgccttc aacagcttcc tggcaaagtg gatccggctc
      901 ttaatggttc ccagcggttc ctgcagcata tcggcaattt catggtattt gtacccgtca
      961 aaatacagca ggaacggatt tctgaaaata tccggaaggt tatgaatagc tccctggata
     1021 tcccggatgc ggagattgct ttccgctgta tcggaaatga cggccgactg gctgttattg
     1081 agcagatagt cgttcggcga cgaatcaaaa atggtcttct gctttgactt acggcggtag
     1141 ttattaatga agatattccg catgatcgta tacaaccatg ctttaatatt ggtaccgaca
     1201 ttatattttt ccttattggc aatagcccgg tacagcgttt cctgaaacag gtcctttgcc
     1261 gcctcactat cccgggtcag ggtaatggca aaaggtttca gaaaatccgc gttgcttacc
     1321 aaaagctgat tgaattcaag agctcccatg atctcagata tttaccgtac aaaggtaaaa
     1381 ataatgccaa gaccgaatgt cggcaaaagg acagatggat taatattaac agggggttaa
     1441 aggggacaat tggtcatctg gtatgacaaa caggcctgcg gccggatggg ttttacccat
     1501 ttcccggaaa agttacaaat caggatttaa aagcgctccc gcatccaaca gcctgtttac
     1561 cgtgctattg atgctggaag aggcccgcag gatgttaata tgccggaaat tgtgaaggtc
     1621 ggagccgagg aggctgacat attttttctt gatgaggtag ttggccagtt cgagttgttt
     1681 tttgccgtaa tacccgcaaa gcgaaagcag gttcagctga aaaacacagc ccatatcgtg
     1741 gagttcatca tataattcct tatgcacccc gaagtagaga taacgttcgg gatgcgctat
     1801 aatcggttga tatcctttga tctgtagttg aaatagctta tcgcgaaggt caaggggctg
     1861 gcggatgaaa gaaaactcca ccagcaccag gttgccgctg atggtaagca ggggttcgtc
     1921 tttttccagc agctcgtcta tatgttcatc caggtaatat tctgccgctc cccggaaaac
     1981 gatcttcatt tccgtccctt cgagccgatc ccgcacctgc tgggcgcccc ggtttatgat
     2041 atcgctggta tttctgtaca tttcccattg cacatggggg gtgctgatca gtttccggta
     2101 gcccagctcg tacatcgccc ggatcatgac gatggaagac tccaggtcgg tggccccatc
     2161 atcgataccc gggatcaggt gggaatgcat atccgaaacc agacccgaaa agtcaaccgg
     2221 tgaatcgtct ttttttcgaa ataaacgtaa catagttgcg atactaaaaa atataggccg
     2281 gacctggccg gccggctatt cttcctttaa acagcagccc tctcttttcc ataggctgca
     2341 tatacccttt ggattccctc ctccagcgaa atccttgctt tccagcccaa tgcctccagt
     2401 ttggaaacat ccaggagttt ccggggagtg ccatccggct ttgacagatc ccggcggata
     2461 ctgccctcat acccggtaat tttcccgatc agaccggcca gttccataat gctgagatcc
     2521 tttccggtgc ctatgttcag cagtccggag tcattgtatt cctgcatcag aaaaaggcag
     2581 gcgtcggcca ggtcttccac atgcaggaac tcccgtttcg gttcaccggt tccccagatg
     2641 ctgacaaacg gttctccgtt tattttggct tcatgaaatt tccggatcag ggccggaaga
     2701 acatgtgagt tttgcagatc ataatgatca ttggggccat ataagttggt gggcatcacc
     2761 gaaatgaaat tgcatccgta ctgtgcccgg aaggcatcgc agagtttgat gccggcgatc
     2821 tttgcaatcg cataaggctc attggtatgt tccagcagac cggtaagcag gtattcctct
     2881 ttcaaaggct gcggagccag tttcggataa atacaggatg aaccgaggaa catcagtttt
     2941 ttaacgccgg atttccaggc cgaatggatc acattgttct ggatcatcag gttatcataa
     3001 agaaaatccg cccggtaacg gtcattcgcc aggataccgc ccactttagc tgcggccaga
     3061 aaaacataag ccggttggtt ttcttcaaaa aaagccctta ccgccgccgg gtctttcaga
     3121 tcaagctcct tcgatgttct gtaaatcaga ttattaaacc cttcggcctc cagtttccgc
     3181 aggatggcag aacccaccat gccccggtgg ccggcaacgt aaatcttttc ctgtttattc
     3241 atgaattatt tcctgatctt ctttggtgat aaaatcggtt gattttacgg atgaaaggta
     3301 gatgatagcc ctattcatac tggtttttaa tctcgaagcc cgattcctta aggatttttt
     3361 ccctgcggaa aatctccacg tcggcatgga tcatctcccg gaccagggcc gcgagatcgt
     3421 atttaggctc ccatcccagt ttttcctttg ccttgcgggc atcgccgatg agcagatcca
     3481 cttcggtggg acggaaatat cccggatcca cgacgaccac tttcttacct gtttccacgg
     3541 gatagtccgg attcgtgctg gaaacgactt cggcgtattc cctgtcgtcc tttcccttaa
     3601 aaaccagctg aatgccgagt tcggcaaacg ccattttcac aaaatcgcgc actgtggtgg
     3661 tgacgcctgt cgcgatcaca aaatcttccg ccttctcctg ttgaaggatc agccacatcg
     3721 cttccacata atcctttgca tgcccccagt cgcggcgggc gttcaggtta cccagataga
     3781 gtttatcctg caggcccatt ccgatcttgg ccgctgcccg cgtgatcttg cgggtgacga
     3841 aggtttctcc cctcatcggg gattcgtggt tgaacaggat cccgttcgcg gcaaacatct
     3901 ggtaggcttc ccggtaattg accgcgatcc agtaagcata gagtttggcc accgcatagg
     3961 gagagcgggg ataaaaagga gtggtctccg actggggaac cgcctgggcc agaccatata
     4021 attcggaggt ggaagcctga taaattttcg tttttttcac aagccccagc aatctaacgg
     4081 cctcgagtat ccgaagcata ccgatgccgt ccgcattggc cgtgtattcg ggtgtttcaa
     4141 aactcacctg aacatggctc atggcgccga ggttgtagat ctcgtcgggc tgggtttcct
     4201 ggataatgcg gatgaggtta gtggagtcgg tcaggtcgcc gtaatgcagg cgaaaatgaa
     4261 tgtgcttttc atgcgggtcc tgatagagat gatcgatccg gtctgtatta aacagggagc
     4321 ttcttcgttt aattccatgc acctcgtatc ccttatccag cagaaaactg ctgagatagg
     4381 cgccgtcctg gcccgtgacc ccggtgatta atgcaacttt tgccatagta aattatctga
     4441 ttataaactg agcaatcttc cgcaggggct tcaaatataa agtaaaaaaa taagggtgca
     4501 atccgttcaa acgccaggcc atccggtctt cctggttcgc ccttaaccgg tttctcacgc
     4561 tggcattact ctgaccaccc gttctcattt taacgatgac ttccggaata taaccggcgg
     4621 gcatcttata tttcaacaaa atgcgcaaca tcatctcata gtcggaagcg gagcggaggc
     4681 tggtgtcaaa aagaccgacc cggtcgtaaa cggatttccg tacaaaaaaa gtgggatgcg
     4741 gcggcatcca gccccagtaa aaggatccgg gttgaaaatg cccggacttc caggtgcgtg
     4801 taatccggga agtatcctgt gcatgggtat actgaaggtc tgcataacaa gcctgcattt
     4861 tttcgtcctg aaaagcttcc acgatccggg agatcacccc ggagtgcgca tacacatcat
     4921 ccgaattcag gatgcccaca aggtcgccgc gggaggcgcg gatcccctta ttcatcgcgt
     4981 cgtagattcc ggtatccttc tcgcaaacca ccagggccgt atgggaaaaa ccacggacga
     5041 tcttcagggt ttcatcctgc gattgtccgt ccacgatgat gtgttcaata aagggatagt
     5101 cctgcccgct caccgaacgc atcgtttcct ccaccgtgga agcgctgtta taggtagccg
     5161 ttataatgga aacacttaag ggggacattc cggaaaaagt tttttatcgg gtgatcacac
     5221 agttttcaag caccagcata tccatgtcgg ttctcaaaaa acattccagc gcatgttcgg
     5281 gcgaattcac gatcggttcg ttttcattga aagaggtatt cagcagaacc ggaacgcctg
     5341 ttttctgacg gaaagtttca atcagccggt aataccgggg ggcaacctgt ttgtcaaccg
     5401 actgtagccg gcccgttccg tccacatggg tgacggccgg aagaatggcg tgcttggtgg
     5461 attttatggg gaatactttc tccataaacg gtacttcatc cgttacatca aaatattccg
     5521 acacgtattc cttaagaatg gaaggggcga agggccggaa actttccctt ctcttaattt
     5581 ttgaattcag caggtccttg gcatcgctgc ggcgcgggtc ggcaatgatg gaccgggcgc
     5641 caagtgcccg cgggccgaat tcggcgcggc cgttgaacca tcccaccaca ccggaccgga
     5701 tgagccggtc ggttacaata tcatacaatt catcttccgg gtaccgtttg tagctgatgt
     5761 tccgggattg caggtaggtt tctatttctt cattgctgaa ccggctgccg gtgaaggcgg
     5821 aataaacggc cttctgccgg ggctggccga gtacctggtt gtatacatac agcgcggccc
     5881 ccatggaaat gcccgcatca tgaccggccg atgggatata aatattttcg aaagccgtat
     5941 tccgggtgag cttgccattc gcgacggaat tctgcgctac cccgccggca atgcaaatat
     6001 ttttcagtcc tgtttttttc tgaagggatt caagaatatg aaagatcagc aattccgtga
     6061 tgcgctgcac ggatgcgccc aggtctttgt ggtacgcggt gagcggctca ccgggctgcc
     6121 gcggttcacc gaatttcccg gtcatatatt cggaatatag gggcgcaaca accggaacat
     6181 gatcttcccc ataggaaatg atcccgcttt ttgccgagcg aaaatatttc aggttcaacc
     6241 ggaacaaacc gttggaatcg aagagcaata cctctttcag ccggtccaca tatttgggtt
     6301 cgccgtaagg ggccaggccc atcaccttgt attcgtcccc gtaatgcgga aagccgagca
     6361 gttgcgtgaa ggcagtataa aacaaacctg cactgtgcgg gaaatccacc gaatccaaaa
     6421 cggcgatttt atttcctttt ccggtagcgg tcatcgtggt ggtaaaatcg ccggagccgt
     6481 caatggacag gatcgccgat tcttcgaaag gagaagcaaa aaatgcagag gcaaggtgac
     6541 tgcgatgatg ctccacatga tggatcttcg atttgaacgc tttttcatca agggaaggat
     6601 ccagtgattt aaattccgat tcgagcgaag ccactttttt cgcgttggag atccggtctt
     6661 tcaccgcgcg gaatccgccg cccgggtttt ttaaaagata ggtgattttt cttgcgagtt
     6721 tggcatacgg gtcacggcca atggcgatat gatccacctc atgcagcctg atgccggcct
     6781 ctttcaggca gaattcaacg gcctgggaag gaaaaccggc ccagtgtttc actcttctga
     6841 accgctcttc ttccgttgcg gcaaccaact cgccgtcttt aaaaatggcc gcggaagaat
     6901 ccgcgtgata tgcattgatg cccagtataa acatattcag ctaaataatt tgatatattc
     6961 atttttcaga ttggatgcgt cctggcggtt ttttgcaaac tgcctgcatt tttctatcca
     7021 ggcatcgaag gcttgctggt cggcatccgc agcctccagc aaagcggcaa gccaggcctc
     7081 caggttatcc agggagatat cccagccggt ttgttcgtcc cgtaatccgc gccagggcgt
     7141 ttgatcgctg atgaggaccg gccggccggc ggagagtgct tcaaaaacag cgtggccgaa
     7201 attttcgccc agggtcggca acacaaaaac atgatttgct tccatcgccg ggatcacctc
     7261 tgcgtgcggc aggggaccgc gatacaggac ttctatattc ccgggaagga aatgaataac
     7321 cttttgagcc cgtaaccagt atttttcatc ttccacttcc ccatagatcg taaactgcaa
     7381 ctggatgcgc tccggcacca ggttcagcat tttgaggaaa aacagaatat tcttttttgg
     7441 aataatccgg gaaatgtaaa cacaacgcaa ctcgcccgcc atttttttca ccggaacaga
     7501 atatgctggt accgatccgg aaaaattaga aatcacctct accctcccgg cttttggaaa
     7561 ctgccggagg atatcccgtt tttcctgttc atccgttgca tgaaaatgga tcttccggga
     7621 aatgcccagg acattcagca gggcgataaa caattttttc tttccgggct tgaattttaa
     7681 tgccccttgc tgcagcatgc cccggggagc cagaatgatc tgcgcccgga ttttttttct
     7741 ccataaaaga aacaggggca aaatgctgaa ccggtaagag tacatgctgt tgagataaat
     7801 gaaatccgga ttccggaatt cgataaggtc cgccagcaac cgcctgttca gcgtttcggc
     7861 cgttgcataa taaacgggca ggcccggctc gcgatgggtc cattccccgg gctgaattcc
     7921 cgggtagttc tctgtatcgc ccaggtcccg gtccagggtg agtacggaaa gacgatacga
     7981 ttcatgcatg gccgaaacaa aattccggca ggattgaatc gggccgccgg ccttatatcc
     8041 gggttcatac cagtcggtga gaatgagaat atttcctgga ggcaatctta gattataatc
     8101 ttgaaataac aaacagctct tctcccgaca gatcgtcgac ctgtatttcc tgcttttcca
     8161 aaggtcctat tttatccggt tttatcgggc tgctgccgat gtaacagggg ttatctttaa
     8221 aaacatgctc atagaatccg gcacttaaat aattgggatg cactttgccc caggcacgat
     8281 gaaaggcctg gggaaaccgc tcgcggtagg cggggcttcc gttcgggcaa aaggcaataa
     8341 aataacctcc gttgatcaac agggattttg ccaggcggat catgccccgg atatccggat
     8401 gatgttctat cacatgcgaa gaaaagaaaa tatggttact gccgctcatc cggttttcat
     8461 cgctgaaaat ttccacgtcc aggttcttcc tgccgtaatc agccctcggt tccgaaattt
     8521 catacccctg aacccggtgg ccggcctgtt taaactggta ggtcagataa ccccagctgc
     8581 agccgtagtc aattatgttc agcgatttca caccgggaaa caaccgggca aacaaattca
     8641 ggtatctgtc gccgtttttg ttggccgtct gaaacccttc ccggatcatc cggtctaaca
     8701 tcggtttgtc cggaaggtca gtcgttattt tatccttttc cttgtattct gtttgataaa
     8761 aagcagcatt ggtctctttt ttatccaccg gatatctgaa atacaaatga caattctggc
     8821 attccatcaa acaggtgacc acgtacttcc tgtcaatcgg tttgcaatcc gtacttccgc
     8881 agtaactgca ttgcagtttc tgacccgatg cccggagcga gtcgatcaaa tatcttattt
     8941 tctgttttcg ggtcagcaaa tccggagcgg tttcttaaaa aaataaggcg agtggtaaat
     9001 cttattcaca cccattcttt tccatccagt aatccagcac cacaaaaagc cagatctcct
     9061 gccagcggat atccggatgg ccggacaaaa attgtttcca ggcggatttt aaatgttctt
     9121 cctgaataaa atctctttgg gccatccgat tgatatgctt ttcacaaaag gaccgcagct
     9181 ctttttttat ccattcattc cagggaaaaa caaatccctg ttttttccgg tgaacgattt
     9241 catccggcaa cagcggctta agtgattcca ccagcagact ttttggatat accggcacct
     9301 tgaaatgatc gggcaccgac attacgaatt ctaccaggtc ctgatcaaaa aagggttccc
     9361 tgatctccag ggagtttgcc atactcatct gatccatatc cttaagaagg gtttgctgtg
     9421 tatagccaag atattctgca gcggtaacct gactgaagaa cggcaacctg accagcgcct
     9481 ccctttttga aattaattgc tgtgccagct catgccgggc aggccccttc agcctggtcc
     9541 attccccgag agcacgcggc gacaataatt ggcgcagcac cgggtaggta tattctattg
     9601 ccggtgatgg cagggccagg aactgtttca tccgttctct ttttgctccc ttgcccaaca
     9661 atccggctaa actgtttttg acaatggcgg gtgctttcca aagccatcgc ttttgctgaa
     9721 accggatata gtgttcgaat atcgggtaac cggcaaacaa ttcatctccg cccgtgccgg
     9781 agagcgctac ccgaatcccc tcccggtgca ccgctttgga aacaacatag gtattgatcc
     9841 catccccgct gggaacatcc atggcatcca gtgcgttggt cagctccgtg agcatgactt
     9901 ccggtttcaa ccggatctgc acatgccggg tattgaactt ctttgcgatc agaccggcat
     9961 attccgattc atcatattcc
//
LOCUS       DASYCU010000395         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1100051, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000395 DASYCU010000000
VERSION     DASYCU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100051"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VGM24_07995"
     CDS             <1..827
                     /locus_tag="VGM24_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY4155354.1"
                     /translation="KFVRQAYYHNDSSNYGLPNEFDISFLAPYLNMKADSSNKSNNVM
                     KLANSFIDSNKQTTRISVNMADVGSVRLPKILQSIEKRSFELFDSSKYSIELTGTTVT
                     FLEGSAFIIHGLKQSILWAFVLIAACMLYLFRSVRILICSLLPNLIPLVITAGVMGWA
                     GVPLKPSTVLVFSIALGIAIDITIRFLVSYKQDQLKGEPVKDAVIRTIHSTGISIIYT
                     SLVLIAGFVIFCFSGFGGTQALGWLTSLTLVMATLTNLLFLPALLISISRRKEIDH"
     gene            complement(858..1958)
                     /locus_tag="VGM24_08000"
     CDS             complement(858..1958)
                     /locus_tag="VGM24_08000"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY4155355.1"
                     /translation="MNLSYTNADSLRQPSTGFPGAAPTWVYLEEIVYGRDQAPVMIDS
                     AKLNGSTGSIELRGKGTKEGIYELVFGENALALPLVNDVEHIRIKADLSKRNDFYTVS
                     ESDASKQLQELLNTVGKKNNEIEKSFTRLDSLKQVNAGDSLLLEATSEKNAAILQLNN
                     YLKKFMETTSNGTLGFLALSWASRSFSPAEFDSSMKSLNGKFPGNTFIAEMQKNSSGQ
                     PAADQTSSESWVGKSVPELVLPDVNGNMISVSSFKGKYLLIDFWASWCGPCRMENPNV
                     VEAYNEFKGKNFTILGVSLDKDKDSWKKAIAQDHLTWTHISDLKYWGSQAVQIFGFQG
                     IPFNVLVDPSGKVIAQELRGRDLENKLKEVLN"
     gene            complement(2065..3513)
                     /gene="gatB"
                     /locus_tag="VGM24_08005"
     CDS             complement(2065..3513)
                     /gene="gatB"
                     /locus_tag="VGM24_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY4155356.1"
                     /translation="MSDFEKYETVVGLEVHAQLLTESKLFCGDSARFGAEPNTQISPI
                     TLGYPGTLPVLNKQAVAFAIKMGFACHSEIERHNYFARKNYFYPDLPKGYQITQHTTP
                     ICKGGYVNIISSFIEKRILLNRIHLEEDAGKSIHDQDPRYTCLDFNRAGVPLIEIVTE
                     PCLNSGEEAAAYLTNLRRILRYLEICDGNMEEGSMRCDANISIRKKGETRLGTKVEVK
                     NLNSIRNVKRAIEYEAGRMMEALEKGETIAQQTRRFDALKGNTERMRSKEEANDYRYF
                     PDPDLPPFLVTDLFLQSIRETIPALPEALIGRYVKEYHLPRYDAEVITDDKETADYFE
                     LLVKQTGSFKAAANWLLGPVKSWLNENNLSIRQLPVSPSSLASLIRLVEEGKINFSTA
                     SSRIFPELARGGESDPLELATRLNLLQTGDEDQIHQWVLQALEKMPEKVAEYKKGKKG
                     LLGLFVGEVKKLSRGKADPKLTNQLLIETLEK"
     gene            complement(3681..4748)
                     /locus_tag="VGM24_08010"
     CDS             complement(3681..4748)
                     /locus_tag="VGM24_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY4155357.1"
                     /translation="MKKIMYKIAGAAVICLAFTLGAGAQETETVPPAPPVPPAADLRQ
                     DLNDNQSEIIIRQKGDKDGKVTIEIRNGNFFLNGKPLEKFDDPNIEIEKKIVGSDDAA
                     MLAYASPFRGNYWNQQMFDRDMQEKLRTQQDMQMKLNNRLFNTNSAFLGVSSRRSETG
                     GATVLEVTGSSPAEKAGIKKGDVITKVNDEKIESPENLFESIHQFKAGDKVKITFKRG
                     GREQTVSVVLEKSKNAPFTYEYKNFYKMPDMPNMPGMESPLWGPMPPRLGIKAQDSED
                     GKGVNVLEVTDGSPAEKAGLKKGDIITQFEGNETNNADELIQQVRDSRDKFTVKVKVL
                     RDGKPQELNIKIPRKLKTAEL"
     gene            complement(4882..>5068)
                     /locus_tag="VGM24_08015"
     CDS             complement(4882..>5068)
                     /locus_tag="VGM24_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155358.1"
                     /translation="AAVALGESLLKSGALDASSIIKEQVSPLIHGSGGGQKSLATAGG
                     QDITQLKKVIEEVKNRL"
BASE COUNT         1212 a         1256 c         1122 g         1478 t
ORIGIN      
        1 tgaaatttgt gcgacaggca tattatcaca atgactcttc aaactacgga cttccgaacg
       61 aattcgacat cagctttctg gcgccctatc tgaatatgaa agctgacagc agcaacaaat
      121 ccaacaacgt aatgaaactg gcaaacagtt ttatcgacag caataaacaa accacccgga
      181 tcagcgtgaa catggccgac gtgggcagtg tgcgcttacc aaaaattctt cagtcaatag
      241 aaaaaaggtc gttcgaattg ttcgacagtt caaaatattc gatagagctc acgggaacca
      301 cggtaacctt tctggagggc agtgcattta tcattcacgg actaaaacaa agtattttat
      361 gggcttttgt tctgattgcg gcctgtatgc tttatctgtt ccggtccgtc cggatcctga
      421 tctgctccct gcttccaaac ctcattcccc tggtgattac agcgggtgtg atgggttggg
      481 ccggagtgcc gttaaaacct tccacagtgc tggtgtttag tattgctttg ggaattgcca
      541 ttgatattac catccggttc ctcgtaagtt ataaacagga tcagctgaaa ggcgagcccg
      601 tgaaagacgc ggtgatcaga accattcatt ctaccggcat cagcatcata tatacttcac
      661 tggttttgat tgccggattt gtgatattct gttttagcgg atttggcgga acccaggcat
      721 tgggatggct cacttcgctt acactggtga tggcgacgct cacgaacctg ctgtttttac
      781 ctgcattgtt gatttcgatc agcaggagaa aagaaattga ccattgatga agcaagggaa
      841 aggtttctaa tcgtccatta gttcaacact tccttcagct tattttccag gtccctgccc
      901 cgaagctcct gggcgatgac cttcccggaa ggatccacga gcacattgaa aggaattccc
      961 tgaaaaccga agatctgcac cgcctggctg ccccagtatt tcaggtcact gatgtgggtc
     1021 caggtaaggt ggtcctgggc aatcgccttt ttccaggaat ccttgtcctt atccagcgaa
     1081 acgcccagga tggtgaaatt ttttcctttg aattcattgt atgcttccac cacattggga
     1141 ttctccatgc gacagggacc gcaccagctt gcccagaaat caatcagtaa atatttccct
     1201 ttgaaagatg aaacggatat catattcccg tttacatccg gcagaacgag ctcgggaacg
     1261 gatttcccaa cccaggattc ggatgaagtc tgatctgcag ctggttgccc ggaactgttc
     1321 ttctgcattt ccgcaatgaa ggtatttccg ggaaatttcc cgttaaggct tttcatggac
     1381 gaatcgaatt ctgcgggaga aaagctgcgg gaggcccagc tgagcgccag gaaacccagg
     1441 gtcccgttcg aggtggtctc catgaatttt ttcaggtaat tattcagctg aagaatggct
     1501 gcattttttt ccgaagtcgc ttccaggagg agactgtctc cggcattcac ctgtttcagg
     1561 ctatccagcc gggtaaatga tttttcaatt tcattattct tttttcccac agtgttcagc
     1621 aattcctgca gttgctttga agcatccgat tcgctgactg tataaaaatc atttcttttc
     1681 gaaagatcag ccttaatgcg gatatgttcc acatcgttca ccagcggaag cgccagtgcg
     1741 ttttcgccga agaccagctc ataaattccc tcttttgtgc cctttcccct gagttcgatg
     1801 ctgccggtgc ttccatttag ttttgccgaa tcgatcatta ccggagcctg atcccgtcca
     1861 tacacaattt cttccaggta gacccaggtc ggggcagcgc caggaaatcc cgtggaaggc
     1921 tgtctaaggc tgtcagcgtt ggtataagag aggttcaccc ggaacccttt tttccctgaa
     1981 tttttacacc caaaaagaaa aaaagctgcc aataaaaacg gaatggccaa atattttttc
     2041 aacatatagc ggagattgat atgattattt ttcaagtgtt tcgatcagca attgattggt
     2101 cagtttagga tctgccttcc ccctggatag ttttttcacc tccccgacaa ataatcccaa
     2161 aaggcctttt ttcccttttt tatattccgc caccttctcc ggcatttttt ccaatgcctg
     2221 taatacccat tgatgaatct ggtcttcgtc tcctgtctgc agcagattca accgggttgc
     2281 cagctccagg ggatcgcttt ccccaccgcg ggcaagttcg ggaaagattc tggatgaggc
     2341 cgttgaaaag ttgattttcc cttcttccac caaccggatc agagaggcca ggctggatgg
     2401 cgaaaccggc aactggcgga tggaaagatt attttcattg agccaggact ttaccggtcc
     2461 caacagccaa ttggccgctg ccttaaaaga accggtctgt ttaacaagca actcaaagta
     2521 atcagcggtc tctttatcgt cagtaatcac ttccgcatcg tagcgcggta aatgatactc
     2581 ctttacatac cggccgatga gtgcttcggg taaggccgga atagtttcgc gtatactttg
     2641 cagaaaaaga tcggtcacca gaaagggcgg caggtccgga tccggaaaat accggtaatc
     2701 atttgcttcc tcttttgaac gcatcctttc cgtgtttcct ttcagcgcgt caaaacgacg
     2761 ggtttgttgt gctatggttt ctcccttttc aagcgcttcc atcatccggc cggcttcata
     2821 ttcgatagcg cgctttacat tccgtatgga attgaggttc ttgacttcca ctttcgttcc
     2881 aagccgggtt tccccctttt tacggataga aatgtttgca tcacagcgca tgctgccttc
     2941 ttccatattt ccatcgcaga tctccagata ccggagtatc cggcgcaggt tggttaaata
     3001 ggcagctgct tcctctccac tatttaaaca gggttccgtc acaatttcta taagaggcac
     3061 tccggcgcgg ttaaaatcga ggcaggtata ccggggatcc tggtcatgaa tgctttttcc
     3121 ggcatcctct tccaggtgta tccggttcag gaggattcgc ttttcaatga aagaactgat
     3181 gatgttcacg tatcctcctt tgcaaatggg cgtggtatgc tgggttatct ggtatccttt
     3241 cggaagatcc ggataaaaat agtttttcct cgcaaaatag ttgtggcgct caatctccga
     3301 atgacaggca aatcccattt tgatggcaaa ggccactgcc tgtttattca ataccgggag
     3361 cgttccggga tatcccaggg tgatgggact gatttgggta ttgggctccg ctccaaagcg
     3421 ggcactgtcc ccgcaaaaaa gcttgctttc ggtcaatagc tgggcatgca cttcgagacc
     3481 cacaacggtt tcatattttt caaaatcgct catcagataa ccggctgttc aactttctta
     3541 gtaaagcaaa agcgcaaaga tcgttttata ctgttctttg cgcttgcctg accagttgat
     3601 tcctggcacg aagttaaaaa aaagaccagg ttttccctaa aatcctttgc aggcctgatc
     3661 cggatcaatt cctgctttgt tcaaagttcc gcggtcttaa gcttccgcgg gatcttgata
     3721 ttcaattcct ggggtttgcc atcccggagg actttcactt tcaccgtgaa tttatccctg
     3781 gaatcacgaa cctgttgaat cagttcatcg gcattattgg tctcattgcc ttcaaactga
     3841 gtgatgatat ctcccttttt taatccggct ttttctgccg gagacccgtc cgtcacttcc
     3901 agcacattca cccctttgcc atcttcggaa tcctgggcct ttatccctaa cctgggtggc
     3961 atcgggcccc ataagggtga ttccatacca ggcatatttg gcatgtccgg cattttgtaa
     4021 aaatttttgt attcataggt aaatggggca ttttttgatt tttccagaac aacagaaacc
     4081 gtttgctccc tgcccccccg cttaaaggtt atttttactt tatctcccgc tttgaactga
     4141 tgaatgcttt caaaaagatt ttcagggctt tcaatttttt catcgttcac tttggtaata
     4201 acatcccctt tcttaatgcc ggctttttca gcaggactgg aaccggtcac ctccaaaacg
     4261 gtagctcccc cggtctccga tctgcgggag gagactccca gaaaagcgga attggtatta
     4321 aataaacgat tgttcaattt catttgcata tcctgctggg ttcttagttt ttcctgcata
     4381 tcccggtcga acatttgctg gttccagtag ttcccccgga aaggcgaagc ataagcgagc
     4441 atggcggcgt catcagaacc cactatcttt ttttcaattt caatgttcgg atcatcaaac
     4501 ttctccagcg gcttaccatt cagaaaaaaa ttaccattcc tgatttcaat ggtcactttg
     4561 ccgtccttat cccccttttg acgaataatg atctcggatt gattatcgtt taaatcctgg
     4621 cgaaggtccg ctgccggtgg aacaggcggt gccggaggca cggtttcggt ttcctgggcc
     4681 cctgcaccca gggtaaaagc caggcagatg acagccgctc ccgctatctt gtacatgatt
     4741 tttttcatcg tcaataattt taaataataa actacgttag atttcgtatt tcaaatatag
     4801 gaaatatttt caaatacgaa aatattcgga caactatatc cgggcaaggt ttaacagttt
     4861 attatacccg tgaggaacgg gctataaccg gtttttcacc tcttcaataa cctttttgag
     4921 ttgggttata tcctggcctc cggcggtggc gagggatttt tgtccgcccc cgctgccatg
     4981 gatcagcgga gaaacctgtt ctttaattat actggatgca tccagggctc cggacttcag
     5041 gagcgattct cccaatgcaa ccgctgca
//
LOCUS       DASYCU010000396         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1972131, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000396 DASYCU010000000
VERSION     DASYCU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1972131"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VGM24_08020"
     CDS             <1..1185
                     /locus_tag="VGM24_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEY4155359.1"
                     /translation="GLDTTYCAIYLNRVKGLEVHALTVNTGGFSPEEMNAIEKRAKQL
                     GVASFKSVDVTEEYYQSCIRYLIFGNVLKNATYPLSVSAERMVQAIAVAKYVQELDAA
                     YVAHGSTGAGNDQVRFDMVFQGMVPQAEILTPIRDLKLSREQEISFLKEQGVDMNFEK
                     AKYSINKGLWGTSVGGKETLTSKDYLPESAWPTPVSSTNPEKISLQFEKGELVAVNDK
                     PFDPVKAIQFLQSVAQPYGIGRDIHVGDTIIGIKGRVGFEAAAPLIIIKAHHLLEKHT
                     LTKWQLYWKDQLSAWYGNWLHEGQMLDPAMRNIEKFLADTQTTVSGKVFVTLEPYRFT
                     VTGVESPHDLMSSRFGSYGEMNSGWTGEDVKGFAKIFGNQSSIYHQVNDGAEEIKPDR
                     RK"
     gene            1195..2190
                     /gene="argC"
                     /locus_tag="VGM24_08025"
     CDS             1195..2190
                     /gene="argC"
                     /locus_tag="VGM24_08025"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY4155360.1"
                     /translation="MKKIRIGISGGAGYTGGELIRILLQHPHAEIVFVQSGSNAGKEI
                     SSVHHDLAGDTGLTFTDRLQPDVDLLYLCVGHGAARKFLSENPVDKNIRIIDLSQDFR
                     LSPNNLFPGNEFPFVYGLPELNRDRIRQARNIANPGCFASAIELGLLPLAKKGWLTET
                     FTTGITGSTGAGQGLSPTTHFSWRVNNVQAYKTLTHQHLAEVNQSLDQLQPGFLSASP
                     NLHFVPWRGDFARGIFVSSQLKCSLDLNELTGLYKDFYAGHPFTVLSDQPIFLKQVVN
                     TNKCIIQLEKLDDMLVIHSALDNLTKGASGQAVQNMNLMFGLEECAGLRLKASYF"
     gene            2244..>2727
                     /locus_tag="VGM24_08030"
     CDS             2244..>2727
                     /locus_tag="VGM24_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4155361.1"
                     /translation="MKLFDVYPINNITITRAKGSYLWDDAGQQYLDMYGGHAVISIGH
                     THPHFVSRIIHQLNLVAFYSNSVNIPLQKELAEKLGKVSGKEDYQLFLCNSGAEANEN
                     ALKLASFYNGRKKIVAFNKSFHGRTSLAVAVTDNPAIQAPVNQTENVVFLPFNDEAAL
                     E"
BASE COUNT          743 a          664 c          706 g          614 t
ORIGIN      
        1 ggactcgata ccacgtattg cgcgatttat ctgaacaggg taaaagggct ggaagtccat
       61 gcccttacgg taaatacggg cggattcagt ccggaggaga tgaatgccat tgaaaaaagg
      121 gcaaaacagc tcggcgttgc ctcttttaaa tccgtggatg ttacggaaga atattatcag
      181 tcctgcatac gctacctgat ttttggaaac gttctgaaga acgccaccta tcccttaagc
      241 gtcagcgccg aaagaatggt ccaggccatt gccgttgcca aatatgttca ggaactggat
      301 gcggcttatg tggcgcacgg cagcaccggg gcgggaaatg accaggtccg gttcgatatg
      361 gtctttcagg gcatggtgcc ccaggcggaa attcttaccc ccatccggga tctgaaactc
      421 agcagggaac aggaaatcag tttcctgaag gaacaggggg tcgatatgaa tttcgaaaaa
      481 gcgaaataca gtataaataa ggggctctgg ggaacgtccg tgggcggaaa ggaaaccctg
      541 acttcgaaag actatcttcc cgaaagcgca tggcccactc cggtcagttc cacgaacccg
      601 gaaaagatca gcctgcaatt tgaaaagggc gaactcgtgg ccgtgaatga taaaccgttc
      661 gaccccgtta aagccattca atttttgcag tcggtcgcgc agccttacgg aatcgggcgc
      721 gatatacatg tcggagatac cattatcggg atcaagggcc gggtgggttt tgaagcggca
      781 gcaccgctca tcatcatcaa agcccatcac ctgctggaga aacacaccct gaccaaatgg
      841 caactctact ggaaagacca gctgagcgcc tggtatggca actggctgca tgaaggacaa
      901 atgctggatc ccgccatgcg aaacattgag aaattcctcg cagatacaca aaccaccgta
      961 tcgggaaaag tatttgtgac cctggaaccc taccgtttca cggtgacggg agtggaaagc
     1021 ccgcacgatc tgatgagcag ccgcttcggc tcttacggag aaatgaacag cggatggacg
     1081 ggagaggatg taaaaggctt tgcaaaaatt tttggcaacc agtcgtctat ctatcatcag
     1141 gtcaacgacg gcgcggagga aataaaaccg gacagaagga aataaaagga acagatgaaa
     1201 aaaataagaa tcgggataag cggaggggcc ggatacacgg gtggcgaact gatcaggatc
     1261 ctcctgcagc atccgcatgc cgaaattgta tttgttcaga gcgggagcaa tgccgggaag
     1321 gaaatttcca gtgtgcatca tgatctggcc ggagataccg gtctcacttt tacagaccgg
     1381 ctgcagcctg acgtggacct gctttatctt tgtgtcggcc atggcgcagc cagaaaattt
     1441 ctttcggaaa atccggtgga caaaaacata cgtatcattg acctgtcgca ggatttccgc
     1501 ctttccccga ataatctttt cccgggaaat gaatttcctt ttgtttacgg cttaccggaa
     1561 ttgaaccggg accggatcag acaggcgaga aatattgcca atcccggctg ctttgcctct
     1621 gcgatcgaac tggggctttt gcccctggca aaaaaaggat ggctgaccga aacatttacc
     1681 accggaatta cgggctcaac cggggccgga cagggattat cgccgaccac ccatttttcc
     1741 tggcgggtta acaatgttca ggcatataaa acactgacgc atcagcacct ggctgaagtg
     1801 aatcagagcc tggaccagct tcagccagga tttctatcgg caagccccaa tctgcatttt
     1861 gtgccctggc gcggagattt tgcccgcggg atttttgtga gctcgcagct gaaatgcagc
     1921 cttgatctga atgaattgac cggtctctat aaagatttct atgccggtca tccctttacc
     1981 gtcctcagcg atcagcccat attcctgaaa caggtggtga ataccaataa atgcattatc
     2041 cagctggaga aactggatga tatgctggta atccattccg cgctggacaa tctgaccaag
     2101 ggcgcatccg ggcaggcggt tcagaatatg aacctgatgt tcggactgga agaatgcgcc
     2161 ggactgaggt tgaaagcgag ctatttttga aaattttcga ttctctaaat ccggcatgac
     2221 cgctggtatt aaaatcaggc aaaatgaaac tattcgacgt atatccaatc aataacatca
     2281 ccatcacccg ggcaaaaggt tcttatctct gggacgatgc gggtcagcaa tacctggaca
     2341 tgtatggcgg gcacgcggtg atatccatcg gccataccca tccgcatttc gtatcgcgca
     2401 ttatccatca gctgaacctc gttgcttttt attccaattc cgtcaatatc ccattacaaa
     2461 aagagctggc agaaaaactg ggtaaggtct ccggcaagga agactaccag ttgtttctct
     2521 gcaactcggg tgcggaagcc aatgaaaatg cgctcaagct ggcctctttc tataacggaa
     2581 gaaaaaaaat tgtggcgttt aataaatctt ttcacggaag gacctccctg gcggtcgccg
     2641 tcaccgacaa tccggctatt caggctccgg tgaaccagac tgaaaatgtt gtatttctgc
     2701 ctttcaatga cgaagcggcg ctggaag
//
LOCUS       DASYCU010000397         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3855665, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000397 DASYCU010000000
VERSION     DASYCU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3855665"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VGM24_08035"
     CDS             <1..425
                     /locus_tag="VGM24_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY4155362.1"
                     /translation="NVGSSLIFIKFKNRKEAVVKITDEMRKSHKSAGSSRIFTKVEIE
                     SEYPGGTTGWVDYLIKNLKYPAGAAEKGIQGDVVVQFIVEADGSLTDIHAESGPEPLR
                     PESERVIRESGRWIPAKNNGKVVRAYKKQPIIYKLKSS"
     gene            complement(434..1606)
                     /locus_tag="VGM24_08040"
     CDS             complement(434..1606)
                     /locus_tag="VGM24_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY4155363.1"
                     /translation="MSLYSRRGVSAQKEEVHQATASLDKGLYPFAFCRIYSDFLGKDK
                     DWVNLMHADGAGTKSILAYLYWKETGDISVWRDIAQDAVAMNLDDLLCVGIYDNLLFS
                     STIDRNKKRIPGEVLEAIIQGTQLFFDELKMSGIHIQYLGGETADVGDLVRTIAVNGT
                     MTARWPKSRIVTNEKIAAGDLIVALSSSGRANYEEKYNSGIGSNGLTSARHDVLSEMY
                     GVAYPESFDPGLEKEVVYIGSHKLTDEIKTEKGDTLSIGQLLLSPTRTYAPVIKAVLE
                     NHFDQIHGMIHSSGGGQTKCLKYLPRPLKIIKDHLFDPPAVFQVIQQSSGADDREMYQ
                     VFNMGQRLEIFTDPNSATALINLIQSFGVEARVIGRVEAAAKKELLIDTGNNQIGY"
     gene            1727..>2300
                     /locus_tag="VGM24_08045"
     CDS             1727..>2300
                     /locus_tag="VGM24_08045"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HEY4155364.1"
                     /translation="MQVYRNTDSLPDFQHAVLTIGTFDGVHTGHQAVIAQMKKEAGEI
                     QGESVIITFDPHPRTVVNINSGIRLINTTEEKTELLSDKGIDNLVIVPFTEKFAQLSA
                     EEYISEFLIRYFHPHTIIIGHDHHFGKGRTGNYQFLKNVAEKYHYQLKEIPAHVLDSI
                     SVSSTRIREAVSIGEMETANVLLGYTFFFSG"
BASE COUNT          625 a          561 c          558 g          556 t
ORIGIN      
        1 tgaatgtagg cagctccctg atcttcataa aattcaaaaa cagaaaagaa gcggttgtga
       61 agataacgga tgagatgaga aagtctcata agtctgccgg cagcagccgg atctttacaa
      121 aagttgaaat agaatcggaa tatcccggtg gaacaaccgg atgggttgat tacctcatta
      181 aaaatctcaa atatccggcg ggggctgcgg agaaaggaat ccagggagat gttgtggtgc
      241 aatttatcgt ggaggcagac ggcagtctga cagacataca tgccgaaagc gggccggagc
      301 cgctccggcc tgaatctgaa cgggtgattc gggagagcgg aagatggatt ccggcgaaaa
      361 ataacggaaa ggttgtgagg gcttataaaa agcaaccgat catttacaaa ctgaagtcat
      421 cctgagcatt cagctaatac ccgatctgat tattccccgt atcaatcaac agctcttttt
      481 tcgcagccgc ctccaccctg ccgatcaccc gcgcttcaac gccaaaggac tggatgagat
      541 tgatgagcgc ggttgcggaa ttaggatctg taaatatttc caggcgctgg cccatattga
      601 aaacctggta catctcccgg tcatctgcac ccgaagattg ctgaatgacc tgaaaaaccg
      661 ccggaggatc gaataagtga tccttgataa tctttaaggg gcggggcagg tatttgaggc
      721 acttggtttg cccacccccg ctgctgtgga tcattccatg gatctgatca aaatgatttt
      781 ccaaaactgc tttaatcacc ggggcatagg tccttgtggg gctgagcaac agctgcccga
      841 tggaaagggt atcccctttc tccgttttta tttcatccgt cagtttatgg gaaccgatgt
      901 aaaccacttc cttttccagg ccgggatcaa aagattcagg gtaggcgacc ccgtacattt
      961 cactcagcac atcatggcgc gcactggtta acccattgct gccgatcccg ctgttgtatt
     1021 tttcttcgta attcgcgcgt ccgctgctgg aaagagcaac gatgaggtcc ccggcggcga
     1081 ttttttcatt cgtaacgatg cgtgatttcg gccagcgtgc cgtcatcgtg ccgtttacgg
     1141 cgatcgtgcg caccaggtcg cctacgtctg cggtttcacc acccaggtac tgaatgtgga
     1201 tgccggacat cttcaattca tcaaaaaaca gctgggtgcc ctggatgatg gcttccagca
     1261 cttcgccggg tatccgtttt ttattccggt caattgtaga tgaaaaaagc aggttatcat
     1321 aaatgccgac gcaaagcagg tcatccaggt tcatggctac tgcgtcctgc gcaatgtccc
     1381 gccacacgga aatatcgccg gtttctttcc aatataaata agccagaata ctcttggtgc
     1441 cggccccatc cgcatgcatt agatttaccc agtccttatc ctttccgagg aaatcggaat
     1501 aaattctaca gaacgcaaaa ggataaaggc ctttatcgag ggaagctgtc gcctgatgaa
     1561 cttcttcttt ctgagcagaa acaccgcgcc gggaatagag agacatactg aaaagcaggc
     1621 ttttgttgag ccccaaagct aacccaaagg atgaagccgg aaaacgaaaa acgaaaaatt
     1681 ttaagttgtc ccgggggttt atattatttc gcagcacatt tcacctatgc aggtctaccg
     1741 gaacacagat tctttacccg actttcagca tgccgtactc accatcggaa cctttgatgg
     1801 tgtgcataca ggtcaccagg cggtgattgc ccaaatgaaa aaagaagccg gggagattca
     1861 gggtgaatcc gtgatcatta cttttgaccc gcatccgcgc actgtggtca atataaactc
     1921 cgggatcagg ctcatcaata ccacggaaga aaaaacagaa ctgttgtcgg acaaaggaat
     1981 cgacaacctg gtaatcgttc cctttacgga aaaatttgcc cagctgtccg cggaagaata
     2041 catcagcgag ttcctgatca gatactttca tccgcacacc atcatcatcg gccatgacca
     2101 tcattttggc aagggaagaa cagggaatta tcaattcctg aagaatgttg ccgaaaagta
     2161 tcattaccag ctgaaagaga tccccgccca tgtactggat tcgatttcag taagttccac
     2221 aaggatcagg gaggccgttt ccatcggcga aatggaaacc gcaaatgttt tactgggcta
     2281 cactttcttt ttcagcggta
//
LOCUS       DASYCU010000398         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4292011, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000398 DASYCU010000000
VERSION     DASYCU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4292011"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1026)
                     /locus_tag="VGM24_08050"
     CDS             complement(121..1026)
                     /locus_tag="VGM24_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY4155365.1"
                     /translation="MVNLQDIPIDPLDLPQFRESSLDVRVLRLDKIHPDISGNKWFKL
                     KYYLEEAGLRKMNRLISFGGPYSNHILALACAAHTHGFSSTGYIRGERPVSLSHTLLA
                     ARKYGMDLEFLPRTDYRNKEDPAFLSALQTKFPDALIIPEGACGEAGIRGAEEILSLS
                     DTSAYSHICCAVGTGATLAGLVNASKSRQQITGISVLKGTRDREPLDLSWIKDRRKIS
                     KLKIFHDFHFGGYAAKTGGLLCFMNEVYSKTGIPTDFVYTGKLLYAISELARLNYFQE
                     NSRILIVHSGGMQGNLSLPAGQLQF"
     gene            1150..>1901
                     /locus_tag="VGM24_08055"
     CDS             1150..>1901
                     /locus_tag="VGM24_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY4155366.1"
                     /translation="MKPTLLILAAGMASRYGSMKQVQSFGPSGETIMDYSIYDAIKTG
                     FKKIVFVIRKDFAEDFRQIFEPRLKNRVETAYVYQEMTDFLHGYLPTAGRTKPWGTAH
                     AVLCAKPVISDPFAVINADDFYGRDAFEKAHQFLTTGVSSSRYSIIGYDLARTLSENG
                     SVSRGVCEVDNHNNLVSIHERTKIYRNEQGKITYEEGGKTFFVADDSKVSMNFWCFDP
                     SVFAFIEKIFGEFLSTQGQDPKAEFFIPIIGDR"
BASE COUNT          499 a          457 c          457 g          488 t
ORIGIN      
        1 tttgctgcgg gcaggataaa tagggacagt aaaaacggcg caaacagaaa tcgcatggca
       61 cgaccggtat ctttaagatt agaaccgggc aaagataagg gaagcgacct tttttcccga
      121 ttaaaactgc agttgcccgg cagggaggga gaggtttccc tgcattcccc cggaatggac
      181 aatcagtatc ctgctattct cctgaaaata attcaggcgg gccagttccg aaatcgcata
      241 caggagtttc ccggtatata caaaatcggt cggaatgcct gttttggaat agacttcatt
      301 cataaaacac agcagcccac cggtttttgc tgcataccct ccaaaatgaa aatcgtgaaa
      361 gattttaagt ttggaaattt tcctccggtc tttaatccag gataaatcga ggggttcccg
      421 gtcccgggtg cctttcagca cggaaattcc ggttatctgc tgccgggatt tgctcgcatt
      481 gaccaggccc gccagggttg cacctgttcc cactgcacag cagatatggg aataggccga
      541 cgtgtctgac agggaaagaa tttcttccgc accccggatg ccggcttctc cgcaagctcc
      601 ttccggaatg ataagcgcat ccggaaattt tgtttgtagg gcagataaaa aggcagggtc
      661 ttctttattt ctgtaatccg tgcgcggtaa gaattcaaga tccattccat acttccgggc
      721 cgccagcagg gtatgcgaca ggctcaccgg tctttctccc cttatgtaac cggttgatga
      781 aaagccatga gtatgcgcag cacaggcaag agccaggata tgattggaat agggacctcc
      841 gaaactgatc agccggttca ttttccgaag ccccgcctct tccagataat attttagttt
      901 gaaccactta ttgcccgaaa tgtccggatg tattttatca agtctcagga cacgcacgtc
      961 caggctgctt tcccggaatt ggggaagatc cagcggatcg atggggatat cctgcaaatt
     1021 aaccacaggt cccccaaatg aattaatctt atccgcgttt cccattatta tttattttcg
     1081 cagcgcagta aaattacgga accggctgac ttgcttaaaa accaagcatt caaaagtaaa
     1141 aaatagcgga tgaaaccaac tttattgatc cttgcagcgg gaatggcttc ccgatacgga
     1201 agtatgaaac aggttcagtc cttcggccct tcaggagaga ccatcatgga ttattccatt
     1261 tacgatgcca tcaaaaccgg ctttaagaag atcgttttcg ttatccgcaa agactttgcc
     1321 gaggatttcc ggcagatttt cgagccccgg ttgaagaaca gggtggaaac cgcctatgtg
     1381 tatcaggaaa tgactgattt tctgcatggg tatctcccaa cggcaggccg tacaaagccc
     1441 tggggcacgg ctcatgccgt cctttgcgca aaaccggtca tctccgatcc gtttgctgtt
     1501 atcaatgcgg acgattttta tggaagggat gcttttgaaa aagcccatca gttcctgacc
     1561 accggggtca gcagtagccg gtacagtatc atcgggtatg acctggccag gaccctttcg
     1621 gaaaacggtt cggtcagccg gggcgtttgt gaggtagata accataacaa tctcgtttcc
     1681 attcatgaaa ggaccaaaat ctaccggaac gagcagggaa agatcactta tgaagaaggc
     1741 ggcaaaacct tttttgtggc cgatgattca aaagtttcca tgaacttctg gtgttttgat
     1801 ccctcggtat ttgcttttat cgaaaaaatt tttggagaat ttctttccac ccagggtcag
     1861 gatcccaagg ctgaattttt tattcccatc atcggagacc g
//
LOCUS       DASYCU010000399         3666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3566813, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000399 DASYCU010000000
VERSION     DASYCU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3566813"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VGM24_08060"
     CDS             complement(<1..483)
                     /locus_tag="VGM24_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HEY4155367.1"
                     /translation="MMPRKIVYLINPISGTKKKEQVRELISRKTREQGFDFEIFSSQE
                     DGEYDFLRKKVKEEAVTDVVICGGDGTVNGVVQALQKIRVNIGIIPMGSGNGLAFAAR
                     IPKQITRALEIIFNGKAHATDAFYINEKFSCMLCGIGFDALVAHEFAKQKKRGLQTYI
                     R"
     gene            619..1743
                     /locus_tag="VGM24_08065"
     CDS             619..1743
                     /locus_tag="VGM24_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY4155368.1"
                     /translation="MTAPKQRFLALDVFRGMTICFMIIVNTSGSQFSYAPLEHSRWNG
                     FTPTDLVFPSFLFAVGNAMSFVMSRWDTLNQGQVLAKIFKRTFIIFLCGFLLYWFPFF
                     EYNEAHHLVLSPFSHTRIMGVLQRIALAYCIASLIIYYFKPGTVIAISVSILLLYWAF
                     LRIGGDAPDPLSMTGNLGYKIDVWLMGKNHMYHGEGIAFDPEGWLGTLPSAVNVTFGY
                     LVGKFLQQKGKSFEGLAKLLLLGFAFIVIAYFWDLGFPINKKLWTSSFVVYTVGLDCV
                     IISAIIYVIDFLGRTRWTKFFTVFGKNPLFIYLLSELGATLLYIIQPQPDTDLVSWLY
                     TNIYRHAGLYFGSLLFAVSWMLFCWLIGFYLDKKKIYVRV"
     gene            1837..3651
                     /gene="typA"
                     /locus_tag="VGM24_08070"
     CDS             1837..3651
                     /gene="typA"
                     /locus_tag="VGM24_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY4155369.1"
                     /translation="MEIRNIAIIAHVDHGKTTLVDKILHATKVFRDNQETGELIMDSN
                     DLEKERGITIFSKNAAVVYKGVKINVIDTPGHADFGGEVERVLKMADGVLLLVDAFEG
                     PMPQTRFVLQKALHLNLRPIVVINKVDKPNCRPEEVHEAVFELFFNLDATEAQLDFPT
                     YYGSGRNGWFNDSLTPTEDITPLLDGILKWVPAPVKNEGPLQMQITSLDYSSFLGRIA
                     VGKVARGSIKEGQMIALMQADGSIRKSKVKELYVFESLGKKKASEVLCGDICAVVGLE
                     DFNIGDTIADIENPEALPVISVDEPTMSMMFSINNSPFYGKDGKFVTSRHLRDRLMKE
                     TEKNLALRVEDTGSADSFLVFGRGILHLGILIETMRREGYELTVGQPQVIVKEINGRK
                     NEPFEILVVDTPQEYSGKVIDMVTQRKGELHVMETKGEMQHLEFEIPSRGLIGLRSGM
                     LTNTAGEAVMAHRFSDYRPWKGPIPGRSNGVLIAKNQAATTGYSIDKLQDRGSFFVDP
                     GEEVYVGQIIAEHIKPGDLIVNATEGKKLTNMRASGSDDSVRIIPRIQMTLEECMEYI
                     QQDECIEVTPKNIRLRKTILDEEERKKFQRMLKTEEVG"
BASE COUNT          914 a          889 c          934 g          929 t
ORIGIN      
        1 ccggatatag gtttgcagtc cccgcttttt ctgctttgca aattcatgcg ccaccagggc
       61 gtcaaaaccg atcccgcaca gcatgcagga gaatttctcg tttatataaa aggcatcggt
      121 ggcgtgagcc tttccattaa agataatttc cagcgcgcgc gtgatctgtt ttgggatccg
      181 ggcggcaaaa gcaaggccgt tcccggatcc catcgggatg atcccgatat tcacccggat
      241 tttctggagc gcctgtacta cgccgtttac cgttccgtcc ccaccgcaga tcaccacatc
      301 cgtgacggct tcctccttta ccttcttgcg gaggaaatca tattcaccgt cttcctggct
      361 ggaaaagatt tcaaaatcaa aaccctgttc cctggttttg cgggagatca gctccctgac
      421 ctgttctttt tttttcgttc ccgaaatggg atttatgagg taaacgattt ttctgggcat
      481 cattgcaagt tcgttaaaac agcccttttt atcaataata ccgaaagcct ctgataattg
      541 ccacggcatt atggaataat ggccgaagtt tccgaacttg gatttctctc ttgattgcca
      601 gccaccaaaa tcagtacaat gactgctccc aaacaacgct ttcttgccct ggatgtgttc
      661 cggggaatga ccatctgctt tatgatcatt gtgaatactt ccggaagcca gttcagctat
      721 gcgccgctcg aacattcccg ctggaacggg tttacgccga ccgacctggt atttccctct
      781 tttctttttg ccgtgggcaa cgccatgagt tttgtgatga gccgctggga tacccttaac
      841 cagggccagg tgcttgcaaa gatctttaaa cgaaccttta tcattttcct ttgcgggttt
      901 ctgttgtact ggtttccgtt ttttgaatac aacgaagccc atcatctcgt gctctccccg
      961 ttttcccata cccgcatcat gggcgtattg caaagaattg cgctggctta ttgtattgct
     1021 tccctgatca tttattactt caagcccggc acggtgatcg ccatttcggt atccatttta
     1081 ttattatact gggcattcct gcggattggc ggggatgcgc cggatccact gtccatgacg
     1141 ggcaacctgg gatataaaat agatgtctgg ctgatgggga agaaccatat gtatcacggg
     1201 gaaggcatcg cctttgatcc cgaaggctgg ctgggaacct tgccttcggc cgttaatgta
     1261 actttcggat acctggtggg aaaattcctt cagcagaagg gaaagagctt tgaagggctt
     1321 gccaaattac tgctgctggg ttttgccttt atcgtgattg cctatttctg ggacctcgga
     1381 ttcccgatca ataaaaagct ctggaccagt tcttttgtcg tttatacggt aggactggat
     1441 tgtgtgatca tctcggccat tatttatgtc attgactttc taggaaggac cagatggaca
     1501 aaattcttta ccgttttcgg caaaaacccc ctgtttattt acctgttatc cgaactgggc
     1561 gccacgctcc tttatattat ccagccacag cccgacacgg atctcgtttc ctggctgtat
     1621 actaatattt accgccacgc cggactctat ttcggatcct tactgtttgc cgtctcctgg
     1681 atgttattct gctggctgat cggattttac ctcgacaaaa agaaaattta cgtcagggtt
     1741 taaaacctcc ggattccctt ccattaacca ggaaaaaagc atacctttgc gcgtcgaaaa
     1801 aacggtggct gccgtccagc ttaaagtaag gattatatgg agataaggaa tattgcgatc
     1861 attgcacacg tagaccacgg taaaaccact ttggtggata agatcttaca tgccaccaag
     1921 gtattccggg ataaccagga gaccggggag ctgatcatgg actcaaacga cctggaaaag
     1981 gaaagaggca tcaccatttt cagtaagaat gcagcggtcg tttataaagg tgtcaagatt
     2041 aatgttattg atacacccgg tcacgccgat tttgggggtg aggtggaacg cgtcctgaaa
     2101 atggccgatg gcgtattgtt gctggtggat gcattcgaag gacccatgcc gcaaacccgg
     2161 ttcgtacttc agaaagccct tcacctgaat ttgaggccca tcgtggtgat caacaaggtg
     2221 gacaagccga attgccggcc cgaggaagtg catgaggccg tattcgaact atttttcaac
     2281 ctggacgcca ccgaagccca gctggatttc cctacttatt acggctccgg aagaaatggc
     2341 tggttcaatg attcccttac ccctacggaa gatattaccc ccctgctgga tgggatcctg
     2401 aaatgggttc ctgctccggt aaagaacgaa ggtccgctcc agatgcagat cacctcgctc
     2461 gattattctt cttttctcgg ccggatcgcg gtgggaaaag ttgcccgggg cagcataaaa
     2521 gaggggcaga tgatcgcctt gatgcaggca gatggcagca tccggaaatc caaggttaaa
     2581 gagctctatg tttttgaatc gctgggaaag aaaaaagcca gcgaagtgct ttgcggcgat
     2641 atttgtgccg ttgtcggatt ggaagatttt aatatcgggg ataccattgc ggacattgaa
     2701 aatccggagg cgcttccggt gatcagcgtg gatgaaccga ccatgagcat gatgttttcc
     2761 atcaacaact cgccgttcta tggcaaggat ggaaagtttg tcaccagccg gcatctgcgg
     2821 gaccgcctga tgaaagaaac ggaaaaaaac ctggcactga gagtggaaga taccggaagc
     2881 gctgatagtt tcctggtatt cgggcggggt atcctccacc tgggtatcct gatcgaaacg
     2941 atgcggagag aaggatatga gctcacggtg ggtcagccgc aggttatcgt gaaagaaatc
     3001 aacggcagga aaaatgagcc ctttgagatc ctggtggtgg acacccccca ggaatacagt
     3061 ggaaaagtaa ttgacatggt cacccagcgc aagggcgaat tgcatgttat ggaaaccaag
     3121 ggcgaaatgc agcacctgga atttgaaatc ccgtcccgcg gtcttatcgg gcttcgctcc
     3181 ggcatgctca ccaatacggc cggagaagcc gtgatggcgc accggttcag tgattaccgg
     3241 ccctggaagg gaccgatccc gggaagaagc aacggcgtgc tgatcgcaaa gaaccaggcg
     3301 gccacgaccg ggtattcaat cgataaactc caggaccggg gctccttttt tgtggatccg
     3361 ggagaagaag tgtacgtagg acagatcatt gcggagcata tcaagcccgg agatctgatc
     3421 gtgaatgcca cagaaggcaa gaaactgacc aatatgcgcg cgagcggaag cgatgattcc
     3481 gtccggatca ttccccggat acagatgacc ctcgaagaat gtatggaata tatacagcag
     3541 gacgaatgca tagaagttac ccccaagaac atccgcctgc gcaaaaccat cctggatgag
     3601 gaagaaagaa agaaatttca acgcatgctg aagacggagg aagtgggcta ggatttgatg
     3661 gctgac
//
LOCUS       DASYCU010000400         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2696575, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000400 DASYCU010000000
VERSION     DASYCU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696575"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VGM24_08075"
     CDS             <1..377
                     /locus_tag="VGM24_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155370.1"
                     /translation="SQRYGSSAYAPNELVTDLEKGIWKELSSHSAIDPFRRDLQKEYV
                     ESLISLMNPAQPAIPSNLPRGLVLIFGGDIKNTDIPSIARAHLTALRKKILAAASLSP
                     DTLSRYHLVDVAERIKQALEPK"
     gene            complement(401..1468)
                     /locus_tag="VGM24_08080"
     CDS             complement(401..1468)
                     /locus_tag="VGM24_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY4155371.1"
                     /translation="MKGKIYFLPFFFLLIIKSSIAQPGITEPFPSVKPGVYQVEQIRR
                     KYGMFIHFGINTFWDQEWTDGSKPPASYHPPVVDADEWISTAKQAGMKYVILVTKHVD
                     GFCLWNSKYTNYDVAASGNKTDVVEAVARACKKYNIGLGLYYSLWDRNRNADLADHSQ
                     DSAYNRYIVSQISELVGITSRYTRIVELWLDAGWEKKRERWPISEIYRTVKKSEPQCQ
                     VGVNWSIGTPDNPDPDSAFLLPTRQKAGYPIRYFPSDFRMGDPYLPANPDPKLFTHNG
                     KTYYMPWETTVCISQKWFYNTTDTVDKTPQELFHLYKTATAQDNILILDLPPDRSGRL
                     RERDRQTVFALRKLMEQEAEK"
     gene            complement(1647..2810)
                     /locus_tag="VGM24_08085"
     CDS             complement(1647..2810)
                     /locus_tag="VGM24_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY4155372.1"
                     /translation="MNQFTSPNAWPRPGNIFLLSILCFVFCLGCRKSNIKNKDLRDFK
                     QVNLVANTAAYSPMVIDPTLQNGWGLAWAPSGIAWVNSQNGKLSELYTGEGAIVRPPI
                     RIPSPTDTIGGSPTGIVFSGGAGFTLSNKVAPNFLFVGDYGVLSGWNGAAGNNALRIG
                     NSPSPSSYTGLALATVSGKHYLYGANFGTGRIDVWDTSFMPVSMHFHDPALPSGYSPF
                     NIQSVGSWLFVLYAKVGTDGDEVHGAGLGIVDIFNTDGSFVRRFASRGSLNAPWGVVA
                     TPDNFLEDQDMMKEDDHGNKSGTGKNNMGHDDRDEGSVILVGNLGDGHINVFSMEGEY
                     LGQLQSHNRTIAIEGLWALSFAPTTSTIDQKRLYFTAGPRDEADGLFGYLIKD"
     gene            complement(3046..>3874)
                     /locus_tag="VGM24_08090"
     CDS             complement(3046..>3874)
                     /locus_tag="VGM24_08090"
                     /inference="COORDINATES: protein motif:HMM:NF017330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase domain-containing protein"
                     /protein_id="HEY4155373.1"
                     /translation="KLPFLQKVPFIETIFNAKFREAPSNFKMLSAEEIAQISKDPVSS
                     PLMPKQEPGIKPSCALPYQLYADGNLTKDKKGFQIRMEARNEIFGEKSAGSPFNIYIP
                     VKYAAGENGKSVFFENTGSRSYAVMAGGSLPESWPLQSFENGLYHLRLYGPNGFFREY
                     RGNEKDPDLLIRCEYDRSPLNHRKLSGKLLIMAKNTDNHHSHQLEIRDHSYKTKPVSR
                     QIAPGSTVKILLDQTKSFGWYDYALSVNGFDHFEKRYAGRVETGQDGFTDPFMGRMI"
BASE COUNT          940 a          964 c          963 g         1007 t
ORIGIN      
        1 tctcgcagcg ttacggctct tcggcttacg ctcccaatga actcgtgacg gacctggaaa
       61 aagggatctg gaaggaattg tcttcccatt ccgcgattga cccttttcgc cgcgacctgc
      121 aaaaagagta tgtggaatct ctgatatccc tgatgaaccc cgcccagccg gccattcctt
      181 ccaacctgcc gcgggggctt gtgctgatct ttggcgggga tataaaaaat acggatattc
      241 catccatagc cagggcacac ctgacggcac tgaggaaaaa gatcctggct gcggcatcct
      301 taagtccgga taccttaagc cgttatcact tagtggatgt ggccgaaagg attaaacagg
      361 cactggaacc gaagtgaggg cgagccggtc ataccggtct ttatttttca gcctcctgct
      421 ccatcagctt tcttaaggca aataccgttt ggcggtcccg ttctctcagc cggccacttc
      481 tgtccggagg aagatccaat atcagaatat tgtcctgggc cgttgctgtt ttatataaat
      541 ggaataattc ctgcggcgtt ttgtcaacgg tatcggttgt attgtaaaac catttctgac
      601 tgatgcagac cgttgtttcc cagggcatgt aataggtctt cccgttatgg gtgaacaatt
      661 tcgggtcggg attggcgggc aggtaagggt cgcccatccg gaaatcactg ggaaaatacc
      721 ggatcggata tccggctttc tgcctggtgg gcaataaaaa ggccgagtcg ggatcgggat
      781 tatccggcgt tccgattgac cagtttacgc ctacctggca ttgcggttcc gattttttta
      841 ccgtccggta gatctccgaa atcggccagc gctcccgttt tttctcccaa ccggcatcca
      901 gccataattc cacaatccta gtatacctgc tggtgattcc gaccagttcg ctgatctggc
      961 tgacgatata acggttatag gcactgtcct gggaatgatc agccaggtcc gcattccggt
     1021 tgcggtccca gagagaataa taaagaccca gaccaatatt atattttttg caggccctgg
     1081 ctacggcctc cactacatct gtcttgttgc ctgaagcagc cacatcataa ttcgtgtatt
     1141 tgctgttcca caaacaaaat ccgtctacat gcttggtgac caggatcaca tatttcattc
     1201 cggcctgctt tgctgtactg atccactcgt ccgcatccac caccggagga tggtaagatg
     1261 ccggcggttt gctcccatcc gtccattcct ggtcccaaaa cgtatttatc ccgaaatgaa
     1321 tgaacatacc gtatttccga cggatctgct ccacctggta tacaccgggt tttacagacg
     1381 ggaaaggttc cgtgatcccg ggttgggcga tggatgattt tataatgagt aaaaagaaaa
     1441 acggtaaaaa ataaattttc cctttcatga tgggtttttg actggcatta gtggcgaaat
     1501 gaatatatgg attttccatg aattcacccc gccctgacgg tatcggaaac agagaggcag
     1561 cggatcttta accaggatgg taagaaatca gaaaaggccg ttcaaaataa atgaacggcc
     1621 tttgttccgg tttgttttca gaaaatctaa tccttgatca ggtatccgaa cagaccgtcc
     1681 gcttcatccc ggggacccgc cgtgaaatag agtcgtttct gatcgatagt agaggttgtt
     1741 ggcgcgaagg acaaggccca gagcccctcg atggcgatgg tccggttatg ggattgcagc
     1801 tgccccaggt actctccttc catggaaaac acattgatat ggccgtcacc cagattcccc
     1861 accaggataa cggaaccttc atcccggtca tcatgaccca tattgttttt gcccgtaccg
     1921 ctcttgttcc catgatcatc ttctttcatc atatcctgat cttccaggaa attatcgggt
     1981 gtcgccacca caccccaggg tgcgttcagc gaacctcttg aagcaaatct tcttacaaaa
     2041 gatccgtcgg tattaaagat atctacaatg ccaagacctg caccatgcac ttcatccccg
     2101 tccgtcccga cttttgcata gagcacaaag agccaggagc caacggattg gatgttgaag
     2161 ggtgaatacc cggatggaag ggcaggatca tggaaatgca tggaaaccgg catgaagctg
     2221 gtatcccata cgtcaattct gccggtaccg aaattagctc cgtacagata atgttttccc
     2281 gaaaccgtag ccagggcaag tccggtataa gaagagggag aaggactgtt tccgatccgg
     2341 agagcattat taccggcagc tccgttccag cctgataaaa cgccgtaatc tccgacaaat
     2401 agaaaattcg gcgctacctt attggataag gtaaacccgg cgccacccga gaaaacaatg
     2461 cccgtagggg atccgccaat ggtatcggtg ggtgatggaa tgcggatcgg cggcctgaca
     2521 attgcgcctt ctcccgtata taattcactc agctttccgt tctgggaatt cacccaggca
     2581 ataccgcttg gcgcccaggc caggccccat ccattctgca gggtggggtc aatcaccatc
     2641 ggggaatagg ctgccgtgtt cgccaccagg ttcacctgct taaaatcacg gaggtccttg
     2701 tttttaatgt tcgacttccg gcaccccagg caaaagacaa aacataaaat ggagagcagg
     2761 aagatgtttc ccggcctcgg ccaggcattt ggagaagtaa actggttcat gacttagctt
     2821 ttttagatgg gtgtgttaat gaaaaggcaa cccttccgcc tctacccgga aggaatggcg
     2881 ctgaagaaaa gattacgggt aacggaatcg ggcggccagg gtataggggt atttagccgg
     2941 ctttctacag aaagttctga gtttaggccc ggggcaataa gtactaacgc tcctttggaa
     3001 agaggggttg cgtccgtaag atcattaaaa atgccgttct gaaaactaga tcattctccc
     3061 cataaaaggg tctgtgaatc cgtcctggcc ggtctccacc cgcccggcat agcgtttctc
     3121 aaaatgatca aacccgttta cggaaagcgc atagtcgtac catccgaaac ttttggtttg
     3181 gtccagcagg attttgacgg ttgaacccgg agctatttgt ctggaaaccg gttttgtttt
     3241 ataggaatga tccctgatct ccagttggtg gctgtggtga ttgtctgtat tctttgccat
     3301 gatcagcagt ttaccgctga gcttgcggtg gttgagcggg cttctgtcat attcgcagcg
     3361 gatgagcagg tccggatcct tttcgttacc ccggtattcg cggaagaaac cgttgggtcc
     3421 gtaaagccgg agatgataca atccgttttc aaatgactgc agaggccagg attctggcag
     3481 actgcctcct gccatcaccg cataagacct gcttccggta ttttcaaaga aaactgattt
     3541 tccgttttct ccggcggcat atttgacggg aatgtaaata ttaaagggcg agccggccga
     3601 cttttctccg aaaatttcat tccgcgcttc catcctgatt tgaaaacctt ttttatcctt
     3661 cgttaaattt ccgtctgcat agagctgata aggcagtgca caggatggtt ttatacccgg
     3721 ttcctgtttg ggcatcagcg gagaagaaac ggggtctttg ctgatctgcg ctatctcttc
     3781 tgcggacagc atcttgaaat tggagggtgc ttcccggaat ttggcattga aaatggtttc
     3841 aataaaagga actttctgca ggaagggtag tttt
//
LOCUS       DASYCU010000401         7751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_376227, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000401 DASYCU010000000
VERSION     DASYCU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376227"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VGM24_08095"
     CDS             complement(<1..588)
                     /locus_tag="VGM24_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155374.1"
                     /translation="MKTLSLFAFSFFLVLTTYSQNKVEDHFVIAKGQMPNLTRDKNKT
                     IHMVYGYGDSILHLSSPNGRSFSSPELVAVLPGLQASGMRGPQIAATADGLVVTACNK
                     QGNIYSYKKMGSGPWSDARKVNELSETAKEALMALSADGLDAYAVWLGVKSPRGQNVY
                     GAASTDGGKTWNKNVLVYGNAGSTVCECCKPSVVVR"
     gene            838..1182
                     /locus_tag="VGM24_08100"
     CDS             838..1182
                     /locus_tag="VGM24_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155375.1"
                     /translation="MKTILILSNIAFLAVLIIAPVLRKINLFRLITLIMLTIYSIVVV
                     KLIFLFPVPCLFSLVAGMLAVLSMVFLFRPRSVPEAKGNDHKIRILHWHHQEIKAKKE
                     DLKYIAFKSLGI"
     gene            complement(1193..1753)
                     /locus_tag="VGM24_08105"
     CDS             complement(1193..1753)
                     /locus_tag="VGM24_08105"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY4155376.1"
                     /translation="MEKKRCHWPAEDALMIQYHDEEWGVPVHDDRKLFEFLVLDAFQA
                     GLSWRTVLYKRQAFRKAFSNFDAKKVAKFDEARIETLMQDEGIIRNRLKIKAAITNAQ
                     LFLGIQKEFGSFDEYIWRFTGGRSITNKRRDKKNIPATSAESDEMSTDLRNRGFKFVG
                     STICYAFMQAAGMVNDHLTSCFCYKR"
     gene            complement(1912..3597)
                     /locus_tag="VGM24_08110"
     CDS             complement(1912..3597)
                     /locus_tag="VGM24_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4155377.1"
                     /translation="MKKAPEKLKFGLFILLWLGSCKIKNEEISRENIKALDLKRGELV
                     LCGPADKQLGSVDFEFSGSKKIKSDFNLGVALLHSFEYDEAEKVFAKIIDKEPDCAMA
                     YWGVAMCNYHLLWSSPDLPGLKKGSRAIEIARSLDKGSERESGYINAIGMFYKDWEKV
                     DDRTRRINFEKAMETLYGKYPRDKEAAIFYALALDAAADPADKSYARQKKAGAILNAI
                     YPYEPNHPGIVHYIIHSYDYPGLARLALPAARKYASIAPASAHAQHMPSHIFTRLGLW
                     DESIKSNLVSTSAAKCYAENAGIKGHWDEELHGMDYLVYAYLQKGDDSLAKEQCDYLK
                     TISAVYPANFKVAYAFAAIPARYFLEKKMWKEAADLEARPAAFPWEKFPWQKANIHFA
                     RLLGSVHTGRMDSARAEQKTLQILHDTLAREKDFYKANLVEIQIRASDAWILFKEGKK
                     NKALELMNSAAQMEDATEKSPVTPGELIPARELLGDMLMEMGHAANALEAYQDDLRTH
                     PGRFNGLYGAGLAAEKSGDTGKAEDYYKQLAAIGSARSNRPELAAARVYLSRL"
     gene            3794..4966
                     /locus_tag="VGM24_08115"
     CDS             3794..4966
                     /locus_tag="VGM24_08115"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY4155378.1"
                     /translation="MEYDNDRVVKGTYKAEFYSWFLFVLINPVVNSLGIFPHQMKAWA
                     VLLVIGLVLLPLYVLYSRFIVPKFLFTRNYPAYGILTVLFFFLVHSLLFLLYPLGQSI
                     VPGAGQGYFSYSPVTIARESSWIFISTMLAVSIAYLRTKLGEGETVESIRKENTYYKL
                     RYFRTQLNPHFLFNTLNSIYSLSLSNAEKTPEVVIRLADIMRFLIYECNEDKIPIEKE
                     IEFIRNYIEIERIRYDADIQFTVEGSTEGIMIEPFLFISFIENGFKHAIDTSFAKPFI
                     YITLKVGPDRIALNVINNTSLELETQAKKINGKGLLNSKTVLELLYPDAYALDIIQTE
                     IRERKESKLRLKNAQERLQRLYPDTHTLDVILSNNTFTVSLILITGFLDKLYHYRR"
     gene            4938..5651
                     /locus_tag="VGM24_08120"
     CDS             4938..5651
                     /locus_tag="VGM24_08120"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4155379.1"
                     /translation="MISCIIIEDERLAQEVIKNHLQRAGGFNLAGVYRNAPEAKEALE
                     REEVDLIFLDIQLPGMTGLNFLRSLANPPLVVFTTSYPEYALESYEFNVIDYLLKPVS
                     FERFSKTMAKIIDGKIFKTSSSEMRPFHRDHIFIRSDGKFFRIGFSDILFIEGMKDYL
                     KIHTPDQVIITHQTMGEMEGVIPSGKFLRIHKSYIVAVAHIKAVFGNSVDMGKDLLPV
                     GLNYKERLMNFISGKPPGI"
     gene            5708..>7751
                     /locus_tag="VGM24_08125"
     CDS             5708..>7751
                     /locus_tag="VGM24_08125"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY4155380.1"
                     /translation="MKKLVGFLIFFLLLKVSVAQEVKAAIRTPLFSIQMNDGNSLWTY
                     RTAASGKALAFKPPAFEIDGRLQEARIRSLTAGPVVRLANGEREQIFKGPFSADNTLQ
                     CAVSFRWAEDNPIVRFRYSISSRASHHFSKPAGKDELRYFSLSLASRTRFKEIRLSDF
                     NQKIHATALEEVPVEKKDFEDSASVMGPLLEAGDDRSSFVLGYEHGSQYPDRFLEFCL
                     KPDKSVALQAVKGNYLNGQLLDKDHDYETIWFEAGGIDGNEQRLASLYRTFILKYISQ
                     NLASRKPYIYYDTWGRQERTKWAGGTYLSSMNQETTLKEIEVAHQMGIEVYLLDAGWF
                     MKTGDWQVNSGRAFFPDSLKQVRALLNKYGMKLGLWLSPTVAAVSSDMLRRNKDYQAE
                     WRGKKEEPSAIWETEKSVTLDLVSPYWSDLADELIRLTKALGVTYYTWDGISQYGNDG
                     ANGYHGTTANSPEERADSYAFQLPTYLCKVADKVCAAEPQVIFDMDITEEGRSMGLEF
                     LASGKYLILNNGPYFHNLDISEPWKTPLSNGNLNIFVNPGPARGWFTRTVLTYDKWLP
                     SILFLTYYQTDEPRNSQLINVASLILGQNGLWGEILKTSPAGIRFIDTLLEKYKQVRE
                     DVTVSDPVRTGKPGFSSEIVEKINGQNGRGEVVLFSNGGHYRYITDHRVDKKIWCTD"
BASE COUNT         1969 a         1887 c         1844 g         2051 t
ORIGIN      
        1 ccttacaacg acagacggct tgcagcattc gcaaacggta cttcccgcgt taccatatac
       61 cagcacattt ttattccagg tctttccgcc atctgtggat gcggcgccat atacattttg
      121 tccccggggg cttttcactc caagccatac ggcataggca tccagaccgt ccgcgctcag
      181 tgccattaag gcttctttgg ccgtttcact caactcatta actttccggg catcggacca
      241 tggtccggaa cccattttct tataggaata aatattgccc tgcttattgc atgcggttac
      301 gacaagcccg tcagcggtag cggcgatttg cggtccccgc ataccagaag cctgtagccc
      361 gggcaagacc gcaaccaatt ccggagagga gaacgatcgt ccgttcgggg atgataggtg
      421 taaaatgcta tctccatatc cataaaccat atgaatcgtt ttgtttttat ccctggtcag
      481 gttgggcatc tgccctttgg caataacaaa atgatcttca accttatttt gggaataagt
      541 ggtcagaaca aggaaaaagg agaatgcaaa aagacttagc gttttcataa atgacagagc
      601 gataatggtt aaagtatggg gttatgccca tacaaattta cactatccaa aattgacaaa
      661 taaaaacccg ccgcgatgag gatggactcc tggataattg gagcctaacc ctcccgtttt
      721 ttaaccgtat tcaatcagtt gattttcaat atctaatcct ggctatcccg ggcggggtta
      781 caatttccct ttccgtggtt actaaaggca ggttcattct caaattaaat ccgactcatg
      841 aaaactatcc tcatcctctc caatatcgca tttcttgcgg ttctgataat tgctcccgtg
      901 ctaagaaaaa ttaacctctt ccggttgatc acactgatca tgctgacgat ttacagcatt
      961 gttgttgtaa aactgatctt cctgtttccg gtcccctgcc ttttttcatt agttgccggc
     1021 atgctggccg ttctctccat ggtcttcctg ttccgcccca ggtccgttcc tgaagcaaaa
     1081 ggaaatgatc acaagatccg gattctgcac tggcatcatc aggaaatcaa ggcaaaaaag
     1141 gaagatctca aatacatcgc tttcaaaagt ctcggtatct gacgcggggc tactatcgtt
     1201 tatagcaaaa acaggaagta aggtggtcat tcaccatgcc ggccgcctgc ataaaggcat
     1261 agcaaatggt gcttcctaca aacttaaaac cccggttgcg gagatcggtg ctcatttcat
     1321 ctgactcggc gctggtagcc ggaatgttct ttttatctct ccgtttgttc gtgatgcttc
     1381 tgccgccggt aaacctccag atatattcat cgaaagatcc aaattccttt tgtataccaa
     1441 gaaacaattg cgcattggta atggcggctt ttatttttag ccggttccgg atgatcccct
     1501 catcctgcat cagggtttct attctggctt cgtcaaattt tgcaaccttt ttcgcatcaa
     1561 agtttgaaaa agctttccgg aaagcctgcc gtttatatag aaccgtgcgc cagctcaatc
     1621 ctgcctgaaa agcatccaat acgagaaatt cgaataactt ccggtcatcg tgtaccggaa
     1681 cgccccactc ttcgtcgtgg tactgaatca tcagggcgtc ttctgcgggc caatggcagc
     1741 gtttcttttc catagtcaga aattgcggag atcgtaaaat ggttaattta tgaaggtaag
     1801 atattcggaa atgtttggtg gcaagcgtcc ggattacgat tcgaataatt ctgtaattcc
     1861 tggtttgccg gcttatcccg gtaggcgatg cgcggtgtaa ggccagggaa attacaggcg
     1921 gcttaaataa actcttgcag cggccagttc gggacggttg gaacgggctg aacctattgc
     1981 ggccaattgt ttataatagt cttccgcttt gccggtatct ccggattttt cggccgctaa
     2041 cccggcgcca tagagtccgt taaagcggcc gggatgtgtt ctcaggtcat cctgatatgc
     2101 ttccaatgca tttgccgcat gacccatttc catcaacata tcgcccaata gctcgcgtgc
     2161 cggaattaat tctccgggcg tcaccgggct tttttccgtt gcgtcttcca tttgagccgc
     2221 ggaattcatg agctccaatg ccttgttctt tttcccttcc ttaaacagta tccaggcatc
     2281 ggacgccctg atctgaattt caaccaggtt tgccttatag aaatcttttt ccctggcaag
     2341 ggtatcgtga agtatttgta aggttttctg ttctgcccgg gctgaatcca tcctgccggt
     2401 atgtacagaa cccaggagcc gggcaaaatg aatattcgct ttctgccatg ggaatttttc
     2461 ccagggaaag gcggccgggc gggcctccag gtctgccgcc tccttccaca tttttttctc
     2521 gaggaaataa cgggccggaa tggcggcaaa ggcataagcc actttgaaat ttgccggata
     2581 aaccgcactg atcgttttaa ggtaatcgca ttgttcttta gccaggctat catctccttt
     2641 ttgcaaataa gcatatacca ggtaatccat tccgtgtagt tcttcatccc aatgcccttt
     2701 gatgccggca ttttcagcat aacatttcgc cgcggaagtg gaaaccagat tcgactttat
     2761 gctttcatcc catagaccca gccgggtgaa aatatgggaa ggcatatgct gggcgtgagc
     2821 cgaagccggc gcgatggagg catatttccg tgccgccggc agggccagtc gggcaagtcc
     2881 cggataatca taactgtgaa taatataatg aacgatcccg ggatgattgg gctcatacgg
     2941 atatatagca tttaaaatgg cgccggcctt tttttgcctg gcgtatgatt tatcggccgg
     3001 atcagcagcg gcatcgaggg ccaatgcata aaatatcgcg gcttccttat cccggggata
     3061 ttttccataa agcgtttcca tcgccttttc aaagttgatc cggcgggtgc gatcgtcaac
     3121 cttttcccaa tctttataaa acatcccaat ggcattgata taaccggatt ccctttccga
     3181 tcctttatcc agggaccggg ctatttcaat cgcccgggaa ccttttttaa gtccgggcag
     3241 gtcgggcgat gaccagagca gatggtaatt gcacatagcc actccccaat aggccatcgc
     3301 acaatccggt tctttatcga tgatctttgc gaaaaccttc tctgcctcat cgtattcaaa
     3361 ggaatgcaaa agggctaccc ccagattgaa atcgcttttt atcttttttg agcctgaaaa
     3421 ttcaaaatca accgaaccca attgcttatc agcaggaccg cagagaacga gttcacccct
     3481 tttgaggtcc agggctttta tattctcccg ggatatctcc tcatttttta ttttgcagga
     3541 tcccagccat aataaaatga agagaccaaa tttcaatttt tcgggtgcct ttttcatgtt
     3601 ttcccctgta caccgaattt acgaaatgag gggatctttt tacccggttc cctgaattat
     3661 cgttttcacc tgttttcttc ctgggccaag gctttaatta catcgaagcg ggacccggtt
     3721 ttgtcgaaat tatttccgtt tagcaggcac tgcagtagct ttgaaggagg tttaaccttt
     3781 aaatcatgct ccgatggaat acgataacga ccgggtggta aaaggcacct ataaagccga
     3841 gttctatagc tggtttttgt ttgtgcttat aaatcccgtg gtgaacagcc tggggatctt
     3901 tccccatcaa atgaaggcct gggccgtatt gctggtcata ggccttgttt tattgcccct
     3961 gtatgtcctg tattccaggt ttatcgttcc caaattcctt tttacccgga attatccggc
     4021 ttacggtata ctgacggtct tgttcttttt tctggtacac agtctgcttt ttcttttgta
     4081 tcccctggga caatccattg tcccgggtgc gggccagggc tatttttctt attcacccgt
     4141 gaccattgcc cgggagagca gctggatatt catcagtacg atgctggctg tttccatcgc
     4201 ttacctccgg acaaaactgg gggaagggga aacggtggaa tcgatccgga aggagaatac
     4261 ctattataag ctccggtatt tccgcaccca gttgaacccg cattttcttt tcaataccct
     4321 gaacagcatt tattccctga gtctcagcaa tgcagaaaag acgccggaag tggtgatccg
     4381 gctggccgat atcatgcgct tcctgattta cgaatgcaac gaagacaaga tcccgattga
     4441 aaaagaaatt gaatttatcc ggaattatat tgagatcgaa agaattagat atgatgccga
     4501 catccaattt acggtggaag gcagcacgga aggcattatg atcgaaccct tcctgtttat
     4561 ctcctttatt gaaaacgggt ttaaacacgc catagacact tcctttgcaa aaccattcat
     4621 ttatataacg ctgaaggtgg gcccggaccg gatcgcgcta aatgtaatta acaatacgag
     4681 ccttgaactg gaaacccagg caaagaaaat taatggcaaa ggcctcctga acagcaagac
     4741 cgtgcttgaa ttactttacc ccgatgccta tgcgctggat atcatacaaa cggagatccg
     4801 cgagcgaaaa gaaagtaaat taaggctgaa gaatgcgcag gaaagacttc agcgcctgta
     4861 cccggatact catacacttg atgtaatttt gagcaataat acctttaccg tttcattaat
     4921 cttaataacc ggattccttg ataagctgta tcattataga agatgaaaga ctggcccagg
     4981 aggttattaa aaatcattta cagagagcgg gcgggtttaa cctggcaggc gtttatcgaa
     5041 atgcgcccga agcgaaggag gcgctggaac gggaagaagt ggatcttatt ttcctggata
     5101 ttcaactgcc tggcatgacg ggcctgaatt tccttcgttc tttagccaat ccgccgctgg
     5161 tggtgtttac cacatcatac cctgaatatg ctttggagag ttatgaattc aatgtgattg
     5221 actatttgct gaagccggtt tcttttgaac ggttttccaa aacaatggcc aagataattg
     5281 atggaaaaat tttcaagact tcttccagtg aaatgagacc ctttcaccgg gaccatatat
     5341 tcataagatc ggacggtaaa tttttcagga tcgggttttc tgatatcctg tttatagaag
     5401 gcatgaaaga ctatcttaaa attcataccc cggaccaggt catcatcacg catcagacca
     5461 tgggcgagat ggagggcgtc attccttccg gaaagtttct ccgcatacat aaatcgtata
     5521 ttgtggccgt cgcgcatatc aaagcggtct tcggaaattc ggtcgacatg gggaaggacc
     5581 tgcttccggt cggacttaat tacaaggaaa ggctgatgaa tttcatttcc ggaaaacctc
     5641 ccgggattta agaatgggtt cgggcgcata aaaaagagat ggaagcagga atcgcaattt
     5701 cagatcaatg aaaaaattag ttggattttt aattttcttt ctgctgctaa aggtttctgt
     5761 ggcccaggaa gtcaaagccg ccatccggac tcctttattt tccattcaaa tgaatgatgg
     5821 gaacagtctc tggacctacc ggaccgccgc cagcgggaag gcacttgcct tcaaaccgcc
     5881 cgcttttgaa attgacggcc gccttcagga agcccggatc agaagtctta ccgccggtcc
     5941 ggttgtccgg ctggccaacg gagaaaggga gcagattttc aaagggcctt tttcggctga
     6001 taatacattg cagtgcgccg tcagcttccg ctgggcggaa gacaacccca tcgtaagatt
     6061 ccggtacagt ataagcagcc gggcatctca ccatttcagt aagcccgcgg gtaaggatga
     6121 gctgcgctat ttttccctgt ccctggcctc ccggacccgc ttcaaggaaa tccggctctc
     6181 cgatttcaat caaaagattc atgccaccgc cctggaagaa gtgccggtgg aaaaaaagga
     6241 ttttgaagac agcgccagcg tgatgggtcc tttactggaa gccggggatg accgctccag
     6301 tttcgtgctc ggctatgaac acggttcaca gtacccggac cgttttctgg aattctgcct
     6361 taaaccggat aaatcggtag cgcttcaggc ggtaaaaggg aattatctga acggacaact
     6421 gctggacaag gatcatgatt acgaaaccat ctggtttgaa gcgggaggaa tagacgggaa
     6481 tgaacagcgg ctggcctctc tataccggac tttcattctg aaatatattt cccaaaacct
     6541 ggccagccgc aagccctata tctattacga tacctggggc cggcaggaga gaacgaaatg
     6601 ggccggcggg acctatctgt ccagcatgaa ccaggaaacg acgttgaaag agatcgaagt
     6661 ggcccatcag atgggcatcg aggtttacct gctggatgcg ggttggttta tgaagacggg
     6721 tgactggcag gtcaatagcg gccgggcttt tttccccgac agcctgaagc aggtgagggc
     6781 cctgctgaac aaatacggca tgaaactcgg gctttggctt agccccacgg tggcagccgt
     6841 ctcgagtgat atgctacgga gaaataagga ctatcaggcg gaatggcgcg ggaaaaagga
     6901 agagccttcc gccatctggg aaacggaaaa aagcgtaacg ctggacctgg taagcccgta
     6961 ctggagcgac ttggcggatg aactgatccg gctgacaaaa gcactcggtg ttacctatta
     7021 tacctgggac ggcatcagcc agtatggcaa cgacggagct aacggctatc acggtacaac
     7081 ggcgaacagc cccgaagaac gggcagacag ctatgcgttc cagctgccca cttatttgtg
     7141 taaagtggcg gataaggttt gtgccgccga accgcaggtg atttttgata tggatatcac
     7201 ggaagaaggc aggagcatgg gcctggaatt ccttgcctcc ggcaaatacc taattctgaa
     7261 caatggcccc tattttcaca acctggatat atccgaaccc tggaaaacgc ccttgtcaaa
     7321 cggcaatctg aatatttttg tcaatcccgg cccggcccgc ggctggttta cccggacggt
     7381 acttacctat gacaaatggc ttccttccat actttttctc acctactacc agaccgacga
     7441 accccgaaat tcccagctga tcaatgtggc ctcgctgata ctgggccaga acggactctg
     7501 gggggaaatc ctgaaaactt cgcccgccgg tatccggttc atcgataccc tgctggaaaa
     7561 gtacaaacag gtgcgggagg atgtaacggt tagtgacccg gtgcgtacgg gcaagccggg
     7621 tttcagttcg gaaattgtag aaaagatcaa cggacaaaac ggacggggag aggtggtcct
     7681 gttcagcaat ggcggccatt accgctatat caccgatcac cgcgttgata aaaaaatatg
     7741 gtgcacggac c
//
LOCUS       DASYCU010000402         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1537376, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000402 DASYCU010000000
VERSION     DASYCU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537376"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGM24_08130"
     CDS             <1..235
                     /locus_tag="VGM24_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155381.1"
                     /translation="IKKEEVMKSIFLIGLIILAEACSPTQPMTVTANPDPTTSSSGAT
                     GSAKFTDQMPRKSTDTESHPTDTARIRRDSVPK"
     gene            complement(341..595)
                     /locus_tag="VGM24_08135"
     CDS             complement(341..595)
                     /locus_tag="VGM24_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155382.1"
                     /translation="MQTQKHIQFTNLIKAGGQLHEFNFRRSMGLEGPLFTVDVADPRG
                     DRHYIMFSLEADRWKLKTKTLPAWIEEVIPQIQQAIEARV"
     gene            799..1188
                     /locus_tag="VGM24_08140"
     CDS             799..1188
                     /locus_tag="VGM24_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEY4155383.1"
                     /translation="MNPKGISVLHSQYSVKETIDLLQAFLQNHGATIYSRIDQQDEVE
                     KAGLNLNPLEFILFGNPKVGGPVMAENPLAALDLPLKIIAWEDDQKKVWIAFNDASYI
                     EERYFLPPETGAPLHLENLIRKAVNLI"
     gene            complement(1210..2760)
                     /locus_tag="VGM24_08145"
     CDS             complement(1210..2760)
                     /locus_tag="VGM24_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY4155384.1"
                     /translation="MYFRKYRISALLFLSVPGISLFLIRCGDVPKKVVQSGASLSKNY
                     CGSCHQYPSPEILDSETWVKHVLPEMAPRLGIAVFDDNQYVNNPYSTTATVSFADWLK
                     IVAYYRSGSPKTLKPAFVETKPVQDWAMFTMHRPKDTLSTAATTMVSFDTIGHHIYTS
                     DNQSSDLYQWSDHLALQASRKFKSPAVDVQFNKDSSSGTEHGAFTFIGSLRANDVDNG
                     NLTDIDLDAFKTDTGVVQAVRLDRPVQSLAVDFDKDGLTDQLVCGFGHGRGGLYWFKQ
                     MPGRKFEKKVIWEVPGAIHAVAGDFNQDGWQDIICLFAYGDEGIWMFLNDHHGGFTSK
                     NLLRFPPVYGSSSFQLVDFNHDGKPDILYTAGDNGDYSRVLKPFHGVYIFLNEGDFKY
                     KQAYFYPVNGATKAMAADFNGDGELDIALIAFYCDLKNNPAEGFTYFEQDKPMHFVPH
                     HIPVYHEGRWICMDVKDYNADGRPDIILGNFSLGFLNQKGIKTYWNTHSPYIVLENTG
                     GKPKSKHP"
     gene            complement(2799..>3119)
                     /locus_tag="VGM24_08150"
     CDS             complement(2799..>3119)
                     /locus_tag="VGM24_08150"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEY4155385.1"
                     /translation="MIGSFNDGTIPGKIQFGSAWWFLDQKDGMTRQMNALSNMGLLSR
                     MVGMLTDSRSFLSYPRHEYFRRILCNLFGEEMENGELPDDFPWVGKIIQDICYYNAKT
                     YFGW"
BASE COUNT          868 a          801 c          660 g          790 t
ORIGIN      
        1 gataaagaaa gaagaagtta tgaaaagtat attcctgatc ggcctcatca tactcgcgga
       61 agcctgcagc ccgacccagc caatgaccgt caccgcgaat ccggatccga caacctcttc
      121 ctccggggca acaggctcag cgaaattcac cgaccagatg cccagaaaat ccacggacac
      181 ggaaagtcat cctaccgata ccgccagaat aagaagggat tctgtaccta aataaaaaga
      241 acgaaccggt taattcagcc aacactcctg ccatcggcaa ataccttcaa acggcaaaaa
      301 tgctcttttg ggggatttat tcaaactgcg ccggctcatc tcagacccgg gcttcaatcg
      361 cctgttgaat ttgggggatg acttcttcaa tccaggccgg gagggttttc gttttaagtt
      421 tccagcgatc ggcctcgagg ctgaacataa tatagtgccg gtcgcccctt ggatcggcga
      481 catctaccgt aaataggggt ccttccaggc ccatgcttct tcgaaagtta aattcatgca
      541 attgtccccc ggcttttatc aggttggtaa attggatatg tttttgcgtt tgcataggct
      601 atcagctttc tctaactgga aatgcaaatt gtgtgccgct tggaatttac gatcgatccg
      661 atcagaataa aggcgccaat caggcccttc agatggtctt ccggcgactt ggtaccggaa
      721 ccaggttaac aatgctgcgc aatttgcctt gatatccttg ctgcagctat acctttacag
      781 cagcaaaata aaccgcccat gaatccaaaa ggaataagcg tgctccacag tcaatattcc
      841 gtaaaagaaa ccattgatct tttgcaggct tttttacaaa accacggagc aaccatctac
      901 agcagaattg atcagcagga tgaagtagaa aaagccggcc tcaacctgaa tccgctcgag
      961 ttcatcctgt ttggcaatcc aaaagtcgga ggccccgtta tggccgaaaa tcccctggca
     1021 gcccttgacc tgccgctaaa aatcattgcc tgggaagacg atcaaaagaa ggtttggatc
     1081 gcttttaacg atgcctcata catcgaggaa aggtattttt taccgcctga gacgggcgca
     1141 ccccttcatt tggaaaatct gataagaaag gctgtgaatc tgatttagtc atccttaaac
     1201 gtcatcgatt cagggatgct tagatttagg cttgccaccc gtattctcca ggacaatata
     1261 aggactatgt gtattccagt aagtctttat ccctttctga tttaagaagc caagggagaa
     1321 attacccagg ataatatccg gcctgccatc tgcattataa tccttcacgt ccatgcaaat
     1381 ccaccggcct tcgtggtaaa cgggtatatg gtgcggcaca aaatgcatgg gtttgtcctg
     1441 ttcaaaataa gtaaatcctt ccgccgggtt atttttcaga tcacaataaa atgcgatcag
     1501 cgcaatatcc agttcaccat ccccattaaa atcagcggcc atcgcttttg ttgcgccgtt
     1561 taccggataa aaataggcct gtttatattt gaaatcaccc tcattaagga aaatatatac
     1621 cccatggaag ggttttaata cccttgaata atcgccgtta tccccagcgg tatataatat
     1681 atccggtttg ccatcatgat taaaatcaac cagttgaaaa ctgctggaac cataaaccgg
     1741 cgggaaccgc aataaattct tcgaagtaaa acctccgtga tgatcattca aaaacatcca
     1801 tattccctca tcgccatagg caaaaaggca aataatgtcc tgccagccat cctgattaaa
     1861 atcccccgca acagcgtgaa tggcacccgg aacttcccag atgactttct tctcaaattt
     1921 ccggccgggc atttgtttaa accagtaaag cccgcccctg ccatgtccga aaccacagac
     1981 cagttggtcc gtcaacccgt ccttgtcaaa atccacagcc aatgactgca ccggcctgtc
     2041 cagccggacg gcctgcacaa cgccggtatc cgttttaaaa gcgtcgagat caatatccgt
     2101 taaattcccg ttgtcaacat cattcgctct cagacttccg ataaatgtaa aagcgccatg
     2161 ctccgtaccg ctgctgctgt ctttattaaa ctgaacatct accgccgggg atttaaattt
     2221 ccgcgaagcc tgcaaagcca gatgatcgga ccattgatac agatcggagc tttgattatc
     2281 gcttgtataa atatggtggc caatcgtatc aaaagatacc atggtggttg cagcggttga
     2341 cagggtatct tttggccggt gcatggtaaa catcgcccaa tcctgaacag gtttggtttc
     2401 aacaaaagca ggtttgagtg tttttggact gcccgatcgg taatacgcca ctattttcag
     2461 ccaatcagca aaagagacgg tcgctgtagt ggaatacgga ttattaacat actgattatc
     2521 gtcgaatacc gcaataccca ggcgtggcgc catttccggc aacacatgct ttacccaggt
     2581 ttcgctatcc agtatttccg gcgatggata ctgatggcag ctgccgcaat aatttttgga
     2641 taaggacgcg cccgactgga cgactttttt tggaacgtcg ccgcacctga tcagaaagag
     2701 cgaaatgcca ggcaccgata aaaacagcag cgcacttatc ctatatttcc taaaatacat
     2761 tcatctgaat taagcctcaa aactaacccg ggttccggct accatccaaa atatgttttc
     2821 gcattgtagt agcagatatc ctgaatgatt tttcccaccc aggggaagtc gtccggcagt
     2881 tcgccgtttt ccatttcctc gccaaacagg ttgcacaata ttctccggaa atactcgtgg
     2941 cggggatagg aaagaaatga cctggagtct gtaagcatcc cgaccatcct gctcagcaaa
     3001 cccatattgg ataaggcatt catctgcctg gtcatgccgt ctttctggtc taaaaaccac
     3061 caggcagatc cgaactggat tttaccgggt atcgttccgt cgttaaaact gccgatcat
//
LOCUS       DASYCU010000403         3815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2841534, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000403 DASYCU010000000
VERSION     DASYCU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2841534"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VGM24_08155"
     CDS             complement(<1..710)
                     /locus_tag="VGM24_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155386.1"
                     /translation="MKESLFFDDEFEKHLKDKSDQFKMYPSDKVWNEVYRSLHTRRKR
                     FVAGMSFLIGGILILAGIQLISPAKSPAHVPGVTKAAPQPERASDIDLSAFNPTAFSA
                     SPNASGKPAGNHAQNNKTAFLAFPLLAESTSGLSVEKGSETGIRSEIAAVSKRDRSAD
                     IILPEMASDNSPSLSVKEKGPAQKNLPDLAEVSDAVTASEKTITNRNPMRNDRFSWEI
                     YISPTLNTRHLGGVDYHNV"
     gene            complement(744..1349)
                     /locus_tag="VGM24_08160"
     CDS             complement(744..1349)
                     /locus_tag="VGM24_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4155387.1"
                     /translation="MSKLQGNEVFMTEEAILQGCLKNHAAAQRELYQRYSPKMLAVCF
                     RFAHNREDAEDMLQDGFIKVFLQIHTFQSKGAFEGWIRRIMVHTCINHLKKNKRFNES
                     VDLIQASSAQVREENIPSIVQAKQIVDCIRLLPIGYRTVLNLYAIEGYSHKEISGMLD
                     IEESTSRSQYTRARQMLEDILVRRNLIPEPRGRIDRLTAIQ"
     gene            complement(1396..2925)
                     /gene="gpmI"
                     /locus_tag="VGM24_08165"
     CDS             complement(1396..2925)
                     /gene="gpmI"
                     /locus_tag="VGM24_08165"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEY4155388.1"
                     /translation="MAKKNAILIIMDGWGLGKVDGADAIRQAKTPFVDSLYAKYPNST
                     LVTCGEAVGLPDGQMGNSEVGHLNIGAGRIVYQELQRINVAVRDGSFVRNPVLLQALR
                     LAKNNRKKLHLLGLVSDGGVHSHINHLKAIIDCCKTEGLQNVFVHAFTDGRDTDPKSG
                     LGFIRDLQAHLDSTATGKIATVSGRYYAMDRDKRWERVKLAYDALVNGKGETGKNASA
                     IIEKSYAAGITDEFIKPSVVTDSSGMPLALIQDGDVAVCFNFRTDRCREITEVLTQTD
                     HPELGMRKLFLDYTTMTEYDHHFKNVHVMFENDDLKMTMGEVMAKNQLTQIRIAETEK
                     YPHVTFFFSGGRENPFEGEKRIMIPSPKVATYDLQPEMSAEGICDAILPEIQNKSANF
                     ICLNFANADMVGHTGVFPAAVKAVETVDHCVERVVSAALQNDYLVFITADHGNADYMI
                     NPDGSPNTAHTLNLVPYFIVDNQWRGKVHPGKLADIAPTLLKLMGYEVPKEMTGGILF
                     G"
     gene            3051..3455
                     /locus_tag="VGM24_08170"
     CDS             3051..3455
                     /locus_tag="VGM24_08170"
                     /inference="COORDINATES: protein motif:HMM:NF027349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HEY4155389.1"
                     /translation="MKRHMGWALVAFSLILFSFRAFHKADIYNLDAVATAFRSGNVNQ
                     LSPFLDMRVDISLPGKTDTYSKSQAEMVIRDFFNTNGVKNFKIIQQGESGGFVFCTGM
                     LQTLSGNYRTVLFFRQKGEKQYLQEIRFQSVE"
     gene            3624..>3815
                     /locus_tag="VGM24_08175"
     CDS             3624..>3815
                     /locus_tag="VGM24_08175"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222998.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEY4155390.1"
                     /translation="MNKPVIPFDERLYELIRAAFAEDSGDGDHSTLSSIPADARGKAV
                     LKIKQDGILAGVEVAQKIVQ"
BASE COUNT          872 a          952 c          915 g         1076 t
ORIGIN      
        1 acattatgat aatccactcc gcccaggtgt cgcgtgttca gggtcgggga aatatagatt
       61 tcccagctga agcggtcgtt ccgcataggg ttccggttcg taattgtttt ttctgaagcg
      121 gtcaccgcat cggatacctc cgccagatcg ggcagatttt tttgtgccgg ccctttttcc
      181 ttcacgctca gggaaggact gttatctgaa gccatttcgg gaagaataat atcggcagag
      241 cgatcccttt ttgaaacagc agctatttca gatcttatcc cggtttcaga tcctttttca
      301 acggaaagac cggatgtgct ttctgccaga agtggaaaag ccaggaaagc ggttttgtta
      361 ttctgggcat gattccctgc cggttttccg gatgcgttgg gactggcaga aaaagccgtt
      421 ggattaaatg cgctcaggtc aatatcggat gcccgttccg gttgcggggc ggctttggtt
      481 acccccggca cgtgggcagg actcttcgcc gggctgatca gctgaatgcc ggcgagaatt
      541 aaaatgccgc caatcagaaa gctcatcccg gcaacaaacc ttttgcggcg ggtatgcagt
      601 gaacggtata cttcattcca gaccttgtct gaaggataca ttttgaactg atccgattta
      661 tccttcagat gcttctcaaa ttcatcatca aaaaataaac tctccttcat tatcggatgt
      721 tttggtacgg cttctcacaa gcattattga atcgcagtca gccggtctat tcttcccctg
      781 ggctccggta taagatttct cctgacgaga atgtcttcca gcatttgcct tgcccgggta
      841 tattgtgaac ggctggtgct ttcttcaata tccagcattc ctgaaatttc tttatgggaa
      901 taaccttcaa ttgcatacag gttaagtacc gtacgataac caatgggcag gaggcggatg
      961 caatccacaa tctgttttgc ctgaacgatg gaaggaatat tttcttcccg gacctgtgcc
     1021 gaactggcct gaatgagatc cacactttca ttgaagcgct tatttttttt caggtgattg
     1081 atacaggtgt gcaccatgat ccggcgtatc catccttcaa aagcaccttt actctggaaa
     1141 gtgtgtatct ggaggaagac cttaatgaat ccgtcctgaa gcatatcttc ggcgtcttca
     1201 cggttgtgtg caaacctgaa acagaccgcc agcatcttcg ggctgtatcg ttgatacaat
     1261 tcccgttgag cagcagcatg atttttcaaa cagccttgta aaatggcttc ttcggtcata
     1321 aaaacctcgt tgccctgtaa tttagacaaa tttctgctgt taaagctgca ttgccggaaa
     1381 gattccaggc ggtttttaac cgaataaaat cccgccggtc atctctttgg gcacttcata
     1441 acccatgagt tttaggaggg tgggggctat atcggccagc ttgccggggt ggactttccc
     1501 acgccattga ttatcaacaa taaaataagg aaccaggtta agggtatggg cggtattggg
     1561 cgatccgtcg ggattgatca tatagtcggc gtttccatgg tcggccgtga taaaaacgag
     1621 gtaatcgttc tgcagggcgg cgctgaccac ccgttccacg caatggtcca ccgtttcaac
     1681 cgctttcact gccgcaggaa atacaccggt atgcccgacc atatcggcat tggcaaaatt
     1741 cagacagata aaattggcgg atttattttg tatttccggt agaatggcat cgcagatacc
     1801 ttctgcgctc atttccggtt gcaggtcgta cgtggccact ttgggagacg ggatcatgat
     1861 ccgtttctcc ccttcgaaag gattctccct gcccccgctg aaaaagaacg tgacgtgcgg
     1921 atatttttct gtttctgcaa tgcggatctg ggtaagctgg ttttttgcca ttacttcccc
     1981 catggtcatt ttcagatcat cattttcaaa catcacatgc acgtttttga aatgatggtc
     2041 gtactccgtc atcgtagtat aatccaggaa cagttttctc attcccagct ccgggtgatc
     2101 cgtctgcgtg agcacttcgg tgatttcccg gcaacgatcg gttctgaaat taaaacagac
     2161 cgccacatcg ccgtcctgga tgagcgccag cggcattccg ctggaatccg ttaccacact
     2221 gggttttatg aattcgtcgg taatgccggc ggcataactt ttttcaatta tggccgatgc
     2281 attttttccc gtttcccctt tgccgtttac cagggcatca taggccagtt tcacgcgttc
     2341 ccagcgctta tcccggtcca tggcataata cctgccggac acggtagcga tctttccggt
     2401 ggccgtacta tccaggtgtg cctgcaggtc ccggatgaaa ccgagcccgc ttttcggatc
     2461 ggtatcccgg ccgtcggtga aagcgtggac aaacacgttt tgcaggcctt cggttttgca
     2521 gcagtcaatt attgccttca ggtgattgat gtgtgaatga acgccgccgt cgctgaccaa
     2581 tccgagcagg tgtagttttt tccggttatt ctttgctaac cgcagtgcct gcagcaggac
     2641 cgggttccgg acaaaggaac cgtcccggac cgccacgttg atccgctgca attcctggta
     2701 aacgatacgg ccggcaccga tgttcaaatg gccgacctcg gaattgccca tctgaccatc
     2761 cggaagaccc accgcctcgc cacaggttac cagggtgcta ttgggatatt tggcataaag
     2821 ggaatctaca aagggcgttt tagcctggcg gatcgcatcg gccccatcca ctttacccag
     2881 cccccagcca tccataatga tcaggattgc atttttcttt gccattttct gctctgttag
     2941 gtgtgtaaaa gcctcaaagt tagtgctatt gcattgttta tgccgatttt ccgcacctgg
     3001 ttttactttg gcatattttt agcttcgagg gatgggtaaa cattattctt atgaaacgcc
     3061 atatggggtg ggccctggtt gccttttcgc tcatcctctt ttctttccgg gccttccaca
     3121 aagcagatat ttataatctg gatgcggtgg cgactgcatt tcgttccggc aatgttaatc
     3181 agctctctcc ttttctcgat atgcgggtcg atatcagcct gcctggaaaa accgatacct
     3241 atagcaaatc ccaggcggaa atggtgatcc gtgatttctt caacacaaat ggcgtaaaga
     3301 atttcaagat catccagcag ggcgaaagcg gaggctttgt attttgcacc ggaatgcttc
     3361 agacgctttc cggcaattac cgcaccgtat tatttttccg ccaaaaggga gaaaagcaat
     3421 atcttcagga aatccgtttt cagtcagttg aataggatct ccaggtattt ttcatggccg
     3481 ttgcttcacc tgtttatttc accagccttt attctgtatt catatccgct gatcagccgc
     3541 ctggtataaa ttgccagttt cccatattcg accctcagaa gatgcccgaa ctcgtttaga
     3601 tttgcttttc taaaagataa gcattgaata aacctgtgat tccttttgat gaacgattat
     3661 acgaattaat ccgcgccgca ttcgcagagg acagcggtga cggagatcat tccacgctga
     3721 gctccattcc ggcggatgcc cggggaaaag ccgtgctgaa gatcaagcag gacggcatcc
     3781 tggccggtgt ggaggttgcg cagaaaatag ttcaa
//
LOCUS       DASYCU010000404         6254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_522668, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000404 DASYCU010000000
VERSION     DASYCU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522668"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /locus_tag="VGM24_08180"
     CDS             complement(<1..2087)
                     /locus_tag="VGM24_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4155391.1"
                     /translation="MIRKQLLFALRRLSRHKLTTTINILGLTLGVLSCLVIFLYVDYE
                     FSYDRFHPDRDRIYRVVVSLTSPNGTHNDMASVPPPLAADLRRELTGFSSVAGIYTEN
                     SRVIVPEGGKPARVFSGVRDGEPQHISFADPQYFDIFHYQWLAGNPATALKSPFSVVL
                     AESEARRYFDRGNPEDWMGRTLVYLDSLTVSVSGIVKDWKQNSDFGFADLISYSSIEH
                     SFLQKDMNGWNMFQFNVNAFVKLSPGTSEAQVEKQLPAFFKRHKQLPVEVKGGLTLQP
                     LKDIHFNSSYEDTYGRRAHKPTLYALAGIALFILVIAAINFVNLSTAQAVLRAKEVGV
                     RKVLGSSRGGLVRQFLSETGFIVFASMGLALLLANPVIAALHDFIPEGVRLHPGDPGT
                     WLFIVLTVMVTCLLAGWYPARALSSFLPAISLRGKGSQQLNSKSFLRKGLIVFQFTVS
                     LLFIIGTMMVGRQIHYMINTDLGFKKDAIVTIRLPWDQPGNLKTVLANEISRISGVRQ
                     VCLSTATPEAEGHGGTYLEYKGAADIRIDAGCDRIDSSYMSLYGLTLVAGRNFYATDT
                     ARQGSDPATAGGGPVVSPFRAFILNETAAKALGFRRPADAVGHEVSSGFSGTIGPVLG
                     IVKDFHSTSLHDKIQPFFFTTQAEAGGLLSVKLASADQSAATVKGLLHRMQVLFTKTY
                     PKDLFQARFFDET"
     gene            complement(2240..2716)
                     /locus_tag="VGM24_08185"
     CDS             complement(2240..2716)
                     /locus_tag="VGM24_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4155392.1"
                     /translation="MNERVTLRKTVRSDLDFLFQFQLDEEAGYLAAFMPKNHSDKEAY
                     LEKYTKFLSDPTINMQTVLVDETIAGSIAKFIMQGDTEITYWMDKKFWGKGVGTTVLK
                     NFLTIEKERPIFGRVAFDNFGSQKVLEKCGFIKTGTDIGFASARQTEIVEFIYRLD"
     gene            complement(3151..3969)
                     /locus_tag="VGM24_08190"
     CDS             complement(3151..3969)
                     /locus_tag="VGM24_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155393.1"
                     /translation="MKNSKSLFSLLAGLGLIVCLVSSCKKGNTGPQGPAGTAGAAGAD
                     GAAGAAGVAGADGLAGNTLLNGDGAPADDLGKNGDFYLDITAKALYGPKTASGWGSPV
                     SIQGPQGEAGKDAEILVDTFTIDNGDWSGRGTYWHNVENVPMAGNGFPGMYVTRSNYT
                     IDESILKTGLVMVSYRSSPDFDENRWSPLPYSFYGGTYYYNMDFSLALHQVTIDFYLT
                     RSPYYASASGPFAEDMGGVTLPSYQVKVLTMPASVLVQLGSIMRERKSGTVSGN"
     gene            complement(4168..4797)
                     /locus_tag="VGM24_08195"
     CDS             complement(4168..4797)
                     /locus_tag="VGM24_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4155394.1"
                     /translation="MKNFLLIDDHEVVRSGIKQVLLESFRPCHVYEAWDQSSAIHQLK
                     ERQHQLVVMDVHMPGSNAIELMRHIRAGGPAVPVLIFSIGAEKVYARKFIKAGASGYL
                     TKSSSLTEFKKAVDMILLGRKYMGPFLGFQFFAAPGNDEFSNPFEKLSKREFEIALLL
                     ISGQTVTYISKLLRINTSTVGTHKAHILAKLEISNLTELQEIAKMHRLK"
     gene            5027..>6254
                     /locus_tag="VGM24_08200"
     CDS             5027..>6254
                     /locus_tag="VGM24_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4155395.1"
                     /translation="MLKSYLKTAWRNISRNKLFSILNIAGLATGMAVALLIGLWVYYQ
                     YSYDRFMPGYERAYQVKNNYRSGSGRVNTQDFLPVPLADAMKKDVPGIEHVAVTFSVT
                     YGPMSEVLSVGEKKLSPAEIAVGDDFLKIFKFPLLEGSADQAFSESGSIVLTASTAKA
                     LFGNEQALGKMIRVNDSYGLRVSGVLKDLPANSSFQFGFLTSFADMARGGWVHDGLTD
                     WNQPAFKIYVSLKPHVSYAQVEPQISGLFRKYAPAIYQRSRQQVIMQPIKDWHLYSEY
                     ENGVPAGGLIGYVRIFLVLGALVLLIACINFMNLSTARFEKRAKEVGIRKVVGSTKAW
                     LVMQFLTESVMMALLAFILSLVLVELALPAFNSLAGTPIRIPYANPGFWLLMTGYILF
                     TGCLAGSRPAFYLSSFQ"
BASE COUNT         1468 a         1565 c         1654 g         1567 t
ORIGIN      
        1 atggtttcat cgaagaagcg ggcctggaaa aggtctttgg gatatgtttt tgtaaacagc
       61 acctgcatcc ggtggagcaa tcccttcacg gtggcggcgg attgatccgc gctcgccagt
      121 ttgacactaa gcagtccgcc cgcctctgcc tgtgtagtga aaaagaaggg ttggatcttg
      181 tcatgcaggc tggtcgaatg aaaatctttt acgatgccga ggacgggtcc gatagtccca
      241 ctgaagccgg agctcacctc atgccctacc gcatcggccg gccgccggaa gccgagcgcc
      301 ttggccgccg tttcattaag gatgaaggcc cggaagggag acactaccgg tccgccgcct
      361 gccgtggccg gatcgctgcc ctgcctggcc gtgtctgtgg cgtagaaatt tcgtccggcg
      421 acaagagtca gtccgtataa ggacatatag ctgctgtcta tccggtcgca acccgcgtcg
      481 atccttatat ctgctgctcc cttatattcg agataggtgc cgccatggcc ttcggcttcc
      541 ggagtggcgg tgctgagaca tacctgccgc acgccggaaa tcctggaaat ctcgttggca
      601 agcaccgttt tcagattacc tggctgatcc cagggaagac ggatcgtcac gatggcatct
      661 tttttaaaac ccagatcggt gttgatcata tagtggatct gccggcccac catcatcgtt
      721 ccgataataa agagcaggct cacggtaaac tggaaaacaa tgaggccctt gcgcagaaaa
      781 cttttggagt tcagctgctg gctgcccttc ccccgcagac tgatcgccgg caggaaagag
      841 gagagggccc gcgctggata ccatcccgcc agcaggcagg tgaccatgac cgttagtacg
      901 atgaagagcc aggtaccggg gtcgccggga tgcaaccgga cgccctccgg gataaagtcg
      961 tgcagggccg cgatgacggg attcgccagc agcagcgcca gccccatgga ggcaaagacg
     1021 atgaagccgg tctcgctgag aaattggcgg acaagaccgc cccggctgct gcccagcact
     1081 ttccgcacac ccacttcctt tgcccgcaac accgcctgcg cggtagagag gttgacaaaa
     1141 ttgatggcgg cgatcaccag gatgaacagc gcaatgccgg ccagggcata aagggttggc
     1201 ttatgcgccc ggcggccata agtgtcttcg tagctgctgt taaaatgaat atccttcaga
     1261 ggctggagcg tgaggccgcc tttgacctcc acggggagtt gtttgtgccg tttgaagaag
     1321 gccgggagtt gtttttccac ctgcgcctcg ctggtgccgg gggagagctt gacaaaggcg
     1381 ttcacgttga actggaacat attccacccg ttcatatctt tttgcaggaa actatgctct
     1441 atgctgctat atgaaatgag atcggcgaaa ccgaagtcgc tgttctgttt ccagtctttg
     1501 acgatgccgg agacggagac cgtcagcgag tcgagataga ccagggtccg gcccatccag
     1561 tcttccggat tgccgcgatc aaaatagcga cgcgcttccg attcggccag gaccacggag
     1621 aagggcgatt tcagggcggt ggcggggttg cctgccagcc attggtaatg gaagatgtcg
     1681 aaatattgcg gatcggcaaa actgatatgc tgcggctcgc catcccgtac tccggaaaag
     1741 acgcgcgcgg gtttgcctcc ctcgggtacg atcacccggc tgttttccgt atagatgccg
     1801 gcgacagaac tgaagcccgt aagttcacga cgcagatcgg cggccagggg cggcggcacg
     1861 ctcgccatat cgttgtgcgt tccgttggga gaagtcagcg aaacaaccac ccggtagatc
     1921 cggtcgcgat cgggatggaa gcggtcgtag ctgaactcat agtccacgta aaggaaaatc
     1981 acgaggcagg agagtacgcc gagggtgagt ccgagaatgt tgatcgttgt ggtgagctta
     2041 tgccggctga ggcgacgcag ggcgaataac agttgtttcc ttatcataat gcagaagatt
     2101 gtgctataga ttcagccagt ttggtgccat tttcaaagcg ggctgagaat cagaagctta
     2161 ctattttttt tgagcttacc gcctgttctt ttttgataca gtgggtgtac ggggtgaccc
     2221 gtgcggaacg gagaggggct tagtccagcc tgtagataaa ttcgacgatc tctgtttggc
     2281 gtgcactggc aaaaccgatg tccgtgccgg tttttatgaa gccgcatttt tccaaaactt
     2341 tctgcgaacc gaaattgtca aacgcaacgc gtccgaaaat gggcctttct ttttcgatgg
     2401 tcagaaagtt tttcagtacg gtcgtcccga ccccctttcc ccaaaacttc ttgtccatcc
     2461 aataggtgat ttccgtgtct ccctgcatta tgaattttgc tatgctgccg gcgatggttt
     2521 cgtccaccag gacggtctgc atattaattg tcgggtcgct tagaaatttc gtgtatttct
     2581 ccaggtaggc ttctttgtca ctgtggttct ttggcataaa tgccgccagg tagccggcct
     2641 cttcgtcgag ttgaaactga aagagaaaat ccagatccga tcgtaccgtt ttccggagtg
     2701 tgactcgttc attcattcct tatttttttt tcaaaaataa aacgaataca ggaattaatg
     2761 gaaggggagc gcatgctctg gaattttcct tcccgcagta agctaaaatt caaaatctgc
     2821 attcgattaa atctcggaag cagcttcccg tttcgcacaa ctttgaggtg atttcaccat
     2881 agattgttat ttacaatagc tctattttta ctatccctat ttcgcatgga gcagcttctt
     2941 tgcatcaggt gatcaagacc aaagaacagg cggaccggtt tatgagatcg ctaaaagagg
     3001 cctgaccatt tttgataaat gtttaaaccc gtcttatgga cgggtttttt gattacatag
     3061 atcccaggcc caataaaaaa ccgcgcgggc attcgccggc gcggttttat gaagctgaat
     3121 tatgatacta taaatggtcg gatccgggca ttaattgcct gaaaccgttc cggattttct
     3181 ctccctcata atactgccga gctgtacgag aacgcttgca ggcatcgtca ataccttaac
     3241 ctggtaagac ggaagcgtta caccgcccat atcttcggca aacgggcctg atgcggaagc
     3301 ataatacgga gaccgggtca gatagaaatc aatggtaacc tggtgcaggg cgaggctgaa
     3361 atccatgttg taatagtaag tgcctccgta aaaactataa ggcagcgggg accaccggtt
     3421 ttcatcaaaa tcgggcgatg agcggtagct caccattacc aggccggttt tcaggatcga
     3481 ttcatcaatt gtgtaattgc tgcgggtaac atacataccg gggaaaccgt tacctgccat
     3541 cggcacattt tcgacattat gccaataagt tcctctgcca ctccaatccc cgttgtcaat
     3601 ggtgaatgtg tcaacaagta tttctgcatc cttgccggct tcgccctgag gaccctgaat
     3661 ggatacagga gatccccatc cggaagcggt ttttggacca tataaagctt tggcagtgat
     3721 gtccaggtag aaatcaccgt tctttcccag atcatcggcg ggtgcaccat ctccgttaag
     3781 aagggtgttt cccgcgaggc cgtcagcacc agccacaccg gctgcaccgg ctgcaccgtc
     3841 cgcacctgct gcgccagcgg taccggcagg accctgcgga cctgtatttc cttttttaca
     3901 ggaggaaacc aaacaaacaa taaggccaag accggccagt aaactgaaaa gactttttga
     3961 attcttcatc gtcagatgga tttttttaaa taggttgtgt aatgattatt aatttcaggt
     4021 cagttgtttt attcctgacc cgggaacaaa aatctatctt atcatttcgt cttcctatag
     4081 acggttttct ataggggcta ggggtttacc ccctcgtccg gcaggaagga tataaaggag
     4141 aagaaaggca ggctgtctgg ctgaatttta tttcaagcga tgcattttgg cgatctcctg
     4201 aagttccgtc aggttgctga tctccagttt tgccagtata tgggctttat gagttcccac
     4261 ggtggacgta ttgatgcgga gcagttttga gatatacgtg accgtttgac cgctgatcag
     4321 cagcagggca atttcaaatt cccttttgct taatttttca aacggattgc tgaattcgtc
     4381 attccccgga gcggcaaaaa actgaaatcc caggaacggg cccatatatt tccgccccag
     4441 caggatcatg tcgaccgctt ttttaaattc cgtcagggaa gaactcttgg tgagatatcc
     4501 gctggccccg gccttgatga attttctggc atagaccttt tcggcgccta tggagaagat
     4561 caataccggc acggcaggac cgccggcccg tatgtgccgc atcagttcaa tggcattcga
     4621 accgggcata tggacatcca tgacgacgag ctgatgctgc ctttccttta actgatgaat
     4681 ggccgacgac tgatcccagg cctcataaac atggcagggt ctgaacgatt cgagaaggac
     4741 ctgttttata cctgacctga ctacctcatg gtcgtcaatc aataagaaat tcttcattca
     4801 tgtgtcttgg tgcccgaagc acgccgcgcg aaattaccca actattcaga taaaatgaaa
     4861 actaaacgaa accgtccggc ccgaacgaag tttacggctc aatgaatgag cgccataaac
     4921 gaacgattgt tgtatcgtaa gcgcacagtt taagaaagta caggcgttta gaatattgat
     4981 ctgtaatatt ttaaaatctg gcattttatt ggctccttta acgccgatgc ttaaaagcta
     5041 tctcaaaacg gcctggcgca atatcagccg gaataaactc ttcagcattc tgaatattgc
     5101 cggtcttgca acgggcatgg cagtggcctt actgatcgga ctttgggtat attaccagta
     5161 ttcatacgac cggtttatgc cgggatacga gcgggcttac caggtaaaaa ataactaccg
     5221 cagcggcagc ggccgggtaa atacacagga ctttcttcct gttccgctgg cagatgcgat
     5281 gaaaaaagac gtgcccggga tagagcatgt tgcagttacc ttcagcgtga cctacggacc
     5341 gatgtcggaa gtgctttccg tcggggagaa aaagttgtcg cctgctgaaa tagccgtcgg
     5401 ggatgatttt ttaaagatct tcaaattccc tctgctggag ggatctgccg atcaggcgtt
     5461 cagcgaatcc ggatcgatcg tattgaccgc gtcaacggcg aaagccctgt tcggtaatga
     5521 acaggcgctc ggcaagatga ttcgcgtgaa tgattcctat gggctccggg tgagcggcgt
     5581 gttgaaagac ctcccggcca attcttcctt ccaattcggt ttcctgactt cctttgccga
     5641 catggcccgg ggcggatggg ttcatgatgg cctcacggac tggaaccagc ctgccttcaa
     5701 gatatatgtt tcattgaaac cgcacgtgag ctatgcacag gtggaaccgc agatcagcgg
     5761 actgttcagg aagtatgctc cggctatcta tcagagatcc aggcaacagg tgatcatgca
     5821 gccgataaag gactggcatc tttattcgga gtatgaaaac ggcgttcctg ccggcgggct
     5881 tatagggtat gtgcggatat tccttgtgct cggcgccctt gttttgctga ttgcctgtat
     5941 caattttatg aacctttcca cggcccgctt cgaaaaaagg gccaaagaag tgggcatccg
     6001 gaaagtggtg ggctccacaa aagcgtggct ggtcatgcag ttcctgaccg agtcggtcat
     6061 gatggccctg ctcgctttta tcctgtcttt ggttttggta gaattggcgc tgccggcatt
     6121 taattcgctg gcgggaaccc ccatcagaat tccctatgca aaccccgggt tctggctttt
     6181 gatgaccggg tatatattat ttacgggttg cctcgccggc agcaggccgg cattttatct
     6241 ttcttccttt cagc
//
LOCUS       DASYCU010000405         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3567393, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000405 DASYCU010000000
VERSION     DASYCU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3567393"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="zwf"
                     /locus_tag="VGM24_08205"
     CDS             <1..1140
                     /gene="zwf"
                     /locus_tag="VGM24_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY4155396.1"
                     /translation="IIFYMAVAPQLVPDIAKKLGKKHISPDKACTRIVIEKPFGHDLE
                     SAHELNELLMSMFDEKQIYRIDHYLGKETVQNILALRFANALFEPLWNRNYIDHVQIT
                     AAETVGSEGRGAYYEGAGALRDMVQNHILQLICMIAMEAPVSFDADEIRNKKVDVLNA
                     IRRIGKDEVSKSAVRGQYEAGWMEGEKVPAYREEKNVDPKSSIDTFAALKFYIDNWRW
                     QDVPFYIRTGKRLHQKTTVINIEFRPAPNYAFPPEAAETWRPNRLTISIQPEMDIRLR
                     FQSKRPGQSMTLNPVDMIFRYKDAYDGNEPEAYETLLLDIMEGNATLFMRKDQVEAAW
                     KIIMPVLDAWKNRTPVSFPNYAPGSWGPEDAEALIAKDGHQWIVM"
     gene            1190..1924
                     /gene="pgl"
                     /locus_tag="VGM24_08210"
     CDS             1190..1924
                     /gene="pgl"
                     /locus_tag="VGM24_08210"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY4155397.1"
                     /translation="MNKEVYKDLETLSHELAEWITSLIEETLTRQSRFCIALSGGNTP
                     KKLNELLAGSPYRERIDWKKLQVFFGDERAVPFEDERNNARMAFDTLLNKVDIPKDQI
                     HIMQTQFSPEKAAGEYEKLLRKYFGADETGLPETSFDLILLGMGDDGHTLSLFPGTAV
                     IREEKKWADSLFLPAQDMYRITITKNIANRSEHIVFMISGGDKAKALKEVLNGPYQPE
                     RYPSQVIQPVHGELHFFLDEAAAAEL"
     gene            complement(1921..2241)
                     /locus_tag="VGM24_08215"
     CDS             complement(1921..2241)
                     /locus_tag="VGM24_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEY4155398.1"
                     /translation="MAEDKNAFIHHVFFWLNDPKSTADREKLYEGLRKLSRVSTIKHF
                     HIGRPAGTHRQVIETSYAFSWCIFFANAADQESYQTDPIHLRFVEECSHLWSRVIVYD
                     SVDA"
     gene            complement(2380..>3591)
                     /locus_tag="VGM24_08220"
     CDS             complement(2380..>3591)
                     /locus_tag="VGM24_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4155399.1"
                     /translation="YTVFAKVNGEKFTAFIVEKDFEGFTRGPEEHKMGIKGSSTVQLY
                     FQDCRVPAENVLGEIGRGHVIAFNILNIGRLKLCAAALGAGKRVAGAAIEYANTREQF
                     KTRISQFGAIKNKIAEMAIYIWVAESALYRVSDWIELKKGELLEEGKPVNEAALGAAE
                     EYAIECAMLKVFGSEMLDFVVDEAVQIHGGNGFSDEYPVSRSYRDSRINRIFEGTNEI
                     NRLLIVDMVLKRAAKGRLDILSHAKAVAKELMGIPDFASTDDAPFAGERKTIAQFKKA
                     ILMTAGAAVQKLNERIENEQEILMHIADMAIVCFHAESALLRVIKRAEGKGESGPGFE
                     TDILQTYLYDAADQLNKAGKDAINAFASGDEQRMLLLGLKRYTKTNPVNSKEAKRKIA
                     DLLIAANKYPL"
BASE COUNT          891 a          936 c          881 g          883 t
ORIGIN      
        1 atcatttttt atatggcggt ggctccgcag ctggttcccg atattgccaa gaagctgggt
       61 aagaaacata tttctccgga taaggcctgc acgcgcatcg tgattgaaaa accatttggt
      121 catgacctgg agagcgccca tgagctcaac gaattgctca tgagcatgtt tgatgaaaaa
      181 cagatctacc ggatcgacca ttatctcgga aaagaaacgg ttcagaatat cctggccctt
      241 cgcttcgcca atgctctttt tgaacccctg tggaaccgga actatatcga ccacgtgcag
      301 atcacggcag cagaaaccgt gggatccgaa ggacgcggcg cttactacga aggagccggc
      361 gccctgcgcg atatggttca gaatcatatt ctgcagctta tctgcatgat cgccatggaa
      421 gcccccgttt cctttgatgc cgatgagatc cggaataaaa aagtggatgt gctgaatgcg
      481 atccgccgca tcggaaaaga tgaagtgagc aaatccgccg ttcgcggtca gtatgaggcc
      541 gggtggatgg aaggcgaaaa agtacctgcc taccgggaag agaaaaatgt ggatccgaaa
      601 tcatcgatag ataccttcgc ggcgctgaaa ttttatattg ataactggcg ctggcaggat
      661 gtgcccttct atatccgcac gggcaaaaga cttcatcaga agacaaccgt catcaatatc
      721 gaattccgcc ccgcccccaa ctatgcgttc ccgccggaag ccgcagaaac ctggcgaccc
      781 aaccgcctta cgatcagcat tcagcccgag atggatatcc gcctgcgctt tcaatccaag
      841 cgaccgggac aaagcatgac gctcaacccg gtggatatga tcttccggta taaagacgcg
      901 tacgacggca atgagccgga ggcatatgaa accctgctcc tggatattat ggaagggaat
      961 gcaacgctgt tcatgcggaa ggaccaggtg gaagctgcct ggaaaatcat catgcccgtc
     1021 ctggatgcct ggaaaaacag aacgccggtt tcttttccca attatgcacc cgggtcctgg
     1081 ggaccggaag atgcggaagc cctgatcgcc aaagatggtc atcagtggat cgtgatgtag
     1141 ggaaagcgct ttggccggtc ccggattttg atgctaaaac gttattctta tgaacaagga
     1201 agtttataaa gacctggaga ccctgagcca tgaactggcg gaatggatca cgagcctgat
     1261 agaagaaacg cttacccggc agtccaggtt ctgtatcgcg ctgtccggcg gaaatacgcc
     1321 gaaaaaatta aatgaactgc tggccggatc gccctaccgg gaaagaattg actggaaaaa
     1381 actccaggtg tttttcgggg atgaaagggc cgtgccgttc gaggatgaaa ggaataatgc
     1441 gcgtatggct tttgatacgc tgctgaacaa ggtggatatc ccgaaggatc agatccatat
     1501 catgcagacg caattcagcc ctgaaaaagc agccggagaa tatgaaaagc tgctccggaa
     1561 atatttcggg gcagatgaaa caggcctgcc ggagaccagc ttcgacctca tcctgctggg
     1621 catgggcgac gacgggcata ccctctccct ttttcccggc acagccgtga tccgggaaga
     1681 aaaaaaatgg gccgattccc tttttctccc ggcacaggat atgtaccgta taaccatcac
     1741 caaaaatatc gccaaccggt ctgagcatat cgtgttcatg atcagtggcg gcgacaaggc
     1801 aaaagccctt aaagaagtac tgaacgggcc atatcaaccg gaacggtatc cttcccaggt
     1861 gatccagccc gtgcacgggg aattacactt tttccttgat gaagccgccg cggcggagct
     1921 ttaggcatct accgaatcat atacgatcac gcggctccac agatgggagc attcctcaac
     1981 aaaacgaagg tgtatggggt ccgtctggta gctttcctgg tctgccgcat tggcaaaaaa
     2041 gatacaccag gaaaaggcgt acgaagtttc aatcacctgc cggtgtgtgc cggcggggcg
     2101 cccgatatga aaatgtttga tggtcgaaac tcttgaaagt tttctcagcc cttcataaag
     2161 tttttcccgg tctgcggtac tcttcggatc gttgagccag aaaaatacat ggtggatgaa
     2221 tgcgttttta tcctcagcca ttttaataag caattagttt tctcatgctg gcaactcagg
     2281 ctaaatataa tccatcccgc acattgaacc agatcttctg ctgatttttt ttccccattc
     2341 gggcttacgg cacctacagg aataccctta cccgcctgat tataacggat atttgtttgc
     2401 cgcaatgagc agatcggcga tcttccgttt ggcctccttt gaattgacgg gatttgtttt
     2461 tgtatatctt ttcaaaccga gtaaaagcat ccgttgttcg tcgcccgatg caaatgcatt
     2521 gatcgcatcc tttcctgcct tattcaattg atcggccgcg tcataaagat aggtttgcag
     2581 gatgtccgtt tcaaaccccg ggccgctttc cccttttcct tccgcgcgtt tgataacccg
     2641 cagcagggca ctttccgcgt gaaagcaaac gatcgccata tcggcgatat gcatgaggat
     2701 ctcctgttca ttttcaattc tttcatttag cttttgaacc gcagcacccg ccgtcatcag
     2761 gatggctttt ttaaattgtg cgatcgtttt cctttccccc gcaaatggcg catcatctgt
     2821 tgaagcgaaa tccggaatgc ccatcaattc cttcgcaacg gccttggcat gggaaaggat
     2881 atcaagccgt cccttggctg ctctttttaa aaccatatcc acgatcagga ggcgattgat
     2941 ttcgttggtg ccttcaaaga tgcggttgat gcggctgtcg cggtatgacc tggaaaccgg
     3001 gtactcatcg ctgaagccgt ttccgccatg gatctgtacg gcttcgtcca ccacgaaatc
     3061 cagcatttca cttccgaaca ctttcagcat ggcgcattca atggcgtatt cttccgcagc
     3121 gcccagcgcc gcctcgttca ccggtttacc ttcttccagc agttctcctt ttttcagttc
     3181 gatccagtcg gatacgcggt acagggcgct ttcggccacc cagatataaa tggccatttc
     3241 cgcgatctta tttttgatgg ctccaaactg ggagatcctg gttttaaact gttcccgggt
     3301 attggcgtat tcaatagccg caccggcgac ccgtttcccg gcgcccaatg cggcggcgca
     3361 caatttcaac cggccgatgt tgaggatatt gaaagcgatc acatggcccc ggccgatttc
     3421 tcccaacaca ttttccgccg gcacccggca atcctgaaaa taaagttgaa ccgttgaaga
     3481 acctttgatt cccattttat gttcttcagg tccgcgggtg aatccttcaa aatctttttc
     3541 aacgataaat gcagtgaatt tttctccgtt cactttggca aacaccgtgt a
//
LOCUS       DASYCU010000406         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1973142, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000406 DASYCU010000000
VERSION     DASYCU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973142"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VGM24_08225"
     CDS             complement(<1..860)
                     /locus_tag="VGM24_08225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY4155400.1"
                     /translation="MKIAVIGTGYVGLVTGTCFSETGNDVICVDIDAGKVEKLSAGEI
                     TIYEPGLNKLFLRNLKEDRLKFTTDLAGGIKDAEIIFLALPTPPGADGSADLRFILGV
                     AEQLGGLIRDYKVIVDKSTVPVGTAEKVHAAISKKYPGEFDVVSNPEFLREGVAVEDF
                     MKPDRVVIGTSSDRARKVLEELYSPFVRQGNPIIFMNERSAELTKYAANSFLATKITF
                     MNEIALLCELLGADVDMVRRGVGSDERIGRRFLFPGIGYGGSCFPKDVQALVKSSKEV
                     NYEFQILNAV"
     gene            complement(894..>2041)
                     /locus_tag="VGM24_08230"
     CDS             complement(894..>2041)
                     /locus_tag="VGM24_08230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY4155401.1"
                     /translation="PLIMCIMRPIQMVDLQQQYSRIKQPVDAAIREVLETAAFINGKA
                     VTDFAGSLSAYLNIPHVIPCANGTDALQIALMGLGLKPGDEVITPSFTFIATTEVVAL
                     LGLNPVFAEVDAKTFCLDPVSLEKQISKKTKAIVPVHLYGQSAPMKEIMDIAARHGLH
                     VVEDNAQAIGGDYYFPGGKIQKTGTIGTVGTTSFFPSKNLGAYGDGGALFTRDESLAD
                     RCRMIANHGQKKRYYHELVGCNSRLDSIQAAVLNVKLPLLDEYIGQRIKAADAYDLAL
                     GNHPKIKVPYRAPYSKHVFHQYTILLEGADRNGLNAYLASKKIPSMIYYPVPAHRQQM
                     FAAFHSDRQKLEVTDWLTERVISLPMHTELDEEQIRFICNSVLEFLN"
BASE COUNT          476 a          536 c          508 g          521 t
ORIGIN      
        1 attaccgcat tcaggatctg aaattcataa ttcacttctt ttgaagactt caccagcgcc
       61 tgaacatctt tcgggaaaca gcttccgccg taaccgatac cgggaaaaag aaaacgacgg
      121 ccaatgcgct catcactgcc caccccccgg cggaccatat ccacatccgc tcccaggagt
      181 tcgcagagca gggcgatctc attcatgaag gtgatcttgg tggcaagaaa ggaattcgcc
      241 gcatatttgg tgagttcggc agaacgctca ttcataaaaa tgatcggatt tccctgccgc
      301 acaaaaggac tgtataattc ttccaacact ttcctcgccc ggtccgagga ggtgccgatc
      361 accacccggt cgggtttcat gaaatcttcc accgcaaccc cttcccgtaa aaattccggg
      421 ttggagacca cgtcaaattc gccggggtat ttttttgaga tcgctgcatg gaccttttcc
      481 gcggttccga cgggcacggt gcttttgtct acgatcacct tatagtcccg gatcagtccg
      541 cccagttgtt cggccacacc caggataaac cggagatcgg ccgatccgtc cgctccgggt
      601 ggggtgggga gtgccagaaa aataatttcg gcatccttaa ttccaccggc caggtcggtg
      661 gtgaatttta accggtcttc ctttaaattc cgcaggaaca atttattgag cccgggttca
      721 taaatagtga tctctcccgc agacagcttt tccacttttc ccgcatcaat atccacgcag
      781 atgacatcat tgccggtttc agaaaaacag gttccggtga cgagcccgac ataccctgtt
      841 ccgattactg ctatcttcat aatttgatac cgtttaagta aggggtttaa gaattaattc
      901 agaaattcca gcacgctgtt acagataaag cggatctgtt cttcatccag ttcggtatgc
      961 atagggagtg aaatcaccct ttccgtgagc cagtccgtta cctccagctt ttgccggtca
     1021 gaatggaagg cggcaaacat ctgctgacgg tgtgccggca ccggataata gatcatggaa
     1081 gggatttttt tcgaggccag gtaggcattg agcccgttcc ggtcagctcc ttcaagcaat
     1141 atcgtgtact gatgaaaaac gtgtttggaa tacggcgccc ggtagggaac ttttattttg
     1201 ggatggttcc ccagggccag atcgtaggca tcggccgctt ttatccgctg cccgatatat
     1261 tcatccaaaa gcggcaactt gacattcagc acggcggcct ggatggaatc cagccggctg
     1321 ttacaaccaa cgagttcgtg atagtatctt tttttctgtc cgtgattggc gatcatccgg
     1381 cagcgatctg ccaaagattc atcccgggta aacagcgctc cgccatctcc gtatgccccc
     1441 aggtttttag aggggaaaaa ggaagtggtt cccactgttc cgatcgtacc tgttttctgg
     1501 attttgcctc cggggaaata atagtctccg ccgatggcct gggcgttatc ctccactacg
     1561 tgcaatccgt gtctggcggc aatgtccata atttccttca tgggagcgga ctgcccgtaa
     1621 agatgcaccg gtacgatggc ttttgttttc ttgctgatct gcttttccag ggaaacgggg
     1681 tcgagacaaa atgttttggc atccacttcg gcaaataccg gattaagtcc cagcagggcc
     1741 actacttctg ttgtggcgat aaaggtaaaa gacggggtaa tgacttcgtc gccgggtttc
     1801 agccccaggc ccataagggc gatttgcagg gcatcggtcc cgtttgcgca gggaataaca
     1861 tgcggaatgt tcagataagc ggacaggctt ccggcgaaat ccgtcacggc ctttccgttt
     1921 ataaaagccg cggtttccaa aacctcccgg atagcagcgt ctacaggttg tttgatccgg
     1981 gaatactgct gttggagatc caccatttga attggccgca taatgcacat tattaacggg
     2041 c
//
LOCUS       DASYCU010000407         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3277902, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000407 DASYCU010000000
VERSION     DASYCU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3277902"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VGM24_08235"
     CDS             <1..491
                     /locus_tag="VGM24_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEY4155402.1"
                     /translation="FAKVVKQAQDEGYTEPDPRLDLSGTDVMRKIMILAREAGNVLEM
                     EDISNQVFLPDSCNEGSVADFYKAMEKEEAHFKNLYEAAAGEGKKLKFVASYENDKAS
                     VGLRHIDPDHDFYHLYGKDNIVVFYTDRYPEQPLVIKGAGAGAEVTASGVFADIIRAG
                     SS"
     gene            512..1432
                     /locus_tag="VGM24_08240"
     CDS             512..1432
                     /locus_tag="VGM24_08240"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY4155403.1"
                     /translation="MKEITIHSPGTVANLVCGFDVLGLCLTEPYDIMRVRLLDRKEVI
                     IQTTDDFPLPLDPAQNTAGAPLLEMIAGLEQEAGFEVIITKRIKPGSGIGSSAASAAG
                     AVVAANELLGNIFSKEDLVRFAMFGEKVASGVKHADNIAPCIYGGITLIRSIFPLDIV
                     PIPTPSLFVTVVHPQIEVRTSDSRQILRKEVLLKDAIRQWGNIAGLVAGLMKNDYDLI
                     GRSLEDVIIEPVRSILIPGFHEMKKQCKLAGAIGGGISGSGPSVFMLSRDRPTAIAVE
                     QVMKSLYDRIGLNYYTYVTTINRQGVTFIA"
     gene            1450..>1692
                     /locus_tag="VGM24_08245"
     CDS             1450..>1692
                     /locus_tag="VGM24_08245"
                     /inference="COORDINATES: protein motif:HMM:NF026171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY4155404.1"
                     /translation="MLFYNLRKPSEKVDFRTATIRGQGSDRGLFFPERIPEFSREWLL
                     RIADLSNEEIAFSVIRPYAGDSIPEGPLRKIAAESIA"
BASE COUNT          397 a          422 c          471 g          402 t
ORIGIN      
        1 cgtttgccaa agtggtgaaa caggcccagg acgaaggata tacggaaccg gacccgaggc
       61 ttgacctgag cggcaccgat gtcatgcgca agatcatgat ccttgcccgg gaagccggga
      121 atgtattaga aatggaggat atcagcaacc aggtattcct gccggactcc tgcaatgaag
      181 gttccgtggc ggacttttac aaagcgatgg aaaaagaaga agcgcatttt aaaaatttat
      241 atgaggccgc tgccggcgag ggtaaaaagc tgaaattcgt tgccagctat gaaaatgata
      301 aagcgtccgt tggccttcgg catattgatc cggaccacga tttttatcat ttgtacggga
      361 aggacaatat cgttgttttt tatacagacc ggtatccgga gcaaccgctg gtgataaaag
      421 gggcaggagc cggcgcggaa gtgaccgctt ccggggtatt cgctgatatc atccgggccg
      481 ggagctcctg agccgacagt tttattacat catgaaagag attaccatac attcaccggg
      541 aacggttgcc aacctggttt gcggatttga cgttttgggt ctttgcctta cggagcccta
      601 tgacatcatg cgggtccgcc tgctggacag gaaggaagtg atcatccaga cgacggacga
      661 ctttccgttg ccgctggatc ccgcgcagaa tacggccggc gcccctttgc tggaaatgat
      721 cgcgggcctc gagcaggagg ccggtttcga agtcattatt accaagcgca tcaagccggg
      781 aagtggtatc ggctccagcg cagccagcgc tgccggggca gtggtggctg ccaatgaact
      841 gctggggaat attttttcga aggaagacct ggtgcggttt gccatgtttg gcgagaaggt
      901 cgcctccggt gtgaaacatg cagataatat tgctccctgc atttacggag gcataaccct
      961 gatccggtcc atttttccgt tggatatcgt tcccatcccg acgccttccc tgtttgtaac
     1021 cgtagtccat ccgcagattg aagtgcgtac atccgattcc cgccagatcc tccggaaaga
     1081 ggttttgctg aaagacgcca tccggcaatg gggaaatatt gccggactgg tggccggtct
     1141 catgaagaat gattatgatc tcatcggcag atcgctggag gatgtgatca ttgaacccgt
     1201 tcgctccatc ctgatccccg gatttcatga aatgaaaaag cagtgcaaac tggcgggagc
     1261 cattggggga ggcatttccg gctcgggtcc ttcggtattc atgctgagcc gggatcggcc
     1321 aacggccatt gccgtggagc aggtcatgaa atcactttat gaccggatcg gactgaacta
     1381 ttacacctat gttaccacga tcaaccgcca gggggtaacc tttatcgctt aattaattgc
     1441 taatccgcta tgctttttta caatctccgt aaaccgtccg aaaaagtgga tttccgtacc
     1501 gcgaccatcc ggggccaggg ttcggacagg ggactttttt tcccggaacg cattccggaa
     1561 ttttcgcggg aatggctcct gcggatcgct gatctgtcca atgaagaaat cgccttttcc
     1621 gtaatccgcc cctatgccgg tgattccatt ccggaaggcc cgctgagaaa aatagcggct
     1681 gaatccattg ct
//
LOCUS       DASYCU010000408         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4869114, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000408 DASYCU010000000
VERSION     DASYCU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4869114"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VGM24_08250"
     CDS             complement(<1..413)
                     /locus_tag="VGM24_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155405.1"
                     /translation="MKRIWGIVIGIFIMGGTESQVFHLPVESVCLNGGAYSERFGDAL
                     SFSVNPAVLGLVKTTQAALSSERRWMLNELSYFQAAGSFPAGGGGIGIHFHFTGDAGY
                     SESGLALGYGKSLGRISMGLQFRYDVYHLALYGNKP"
     gene            517..1392
                     /locus_tag="VGM24_08255"
     CDS             517..1392
                     /locus_tag="VGM24_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEY4155406.1"
                     /translation="MQILDGQLVSGSIKDSLKLNVAALQAEGKKTPHLAAVLVGNNGA
                     SETYVAAKVKACSEIGFKSTLVRLEDSVTENKLLSVIRDLNTDPDVDGILVQLPLPKH
                     ISDERVIMAIDPDKDVDGFHPVNAGKLVLGSPAYVSATPHGIMLLLEHYKIETRGRQA
                     AVIGRSNIVGRPMSILLSANSYPGNSTVTLCHSHTHNLEEICLRSDIIVAALGRPGFV
                     KADMVKEGAVIIDVGITRVPDASKKSGFALKGDVDFPAVSPKCSYITPVPGGVGPMTI
                     AALMKNTFSACANRI"
     gene            1405..>1730
                     /locus_tag="VGM24_08260"
     CDS             1405..>1730
                     /locus_tag="VGM24_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEY4155407.1"
                     /translation="MNHSIDIPVQALPVMEAFYTIQGEGFHQGKAAYFIRLAGCDVGC
                     VWCDVKESWDVSAHPLRSVPEIISEAKTHPGRIAVVTGGEPLMHDCLPLTRQLKAAGF
                     QTHMETS"
BASE COUNT          431 a          490 c          443 g          366 t
ORIGIN      
        1 ggtttattcc cgtagagggc aagatgataa acatcgtacc ggaactgcag acccattgat
       61 atccggccca ggctcttccc atagcccagc gcaagcccgc tctcactgta accggcatcc
      121 ccggtaaaat gaaaatgaat accgatgccg cctccgcccg ccggaaatga acccgccgcc
      181 tggaaataag acaactcgtt caacatccac cttctctcgc tcgataaggc cgcctgcgtc
      241 gtttttacca atcccagaac ggctggattc accgaaaagg aaagggcatc gccgaatcgc
      301 tcactgtaag caccgccgtt gagacaaacg gattccaccg gcaggtgaaa gacctgcgat
      361 tcagtcccgc ccattataaa aattccgatt acgattcccc agatccgttt catatcatac
      421 cgccccgggt aaaattaaag gggcaggcga tctggtaaaa aggtaaaatg acccggtaaa
      481 tgaagtttta atacgggagg gaattaactt tgcgccatgc aaattctaga cgggcagctt
      541 gtttccggca gcattaagga ttctcttaaa ttgaatgtgg ccgccctgca ggccgagggg
      601 aaaaaaacgc cgcacctggc tgctgtcctg gtgggaaata acggagccag cgaaacctat
      661 gtggccgcaa aagtgaaagc ctgttcggag atcggtttta aatccaccct ggtccgcctg
      721 gaggattcgg tgacagaaaa caaattactc tccgtgatcc gggacctgaa taccgatccg
      781 gatgtagacg gcatcctcgt gcagctgccc ctgccaaaac atatttccga tgaacgggtg
      841 atcatggcca ttgacccgga taaggatgtg gatggctttc atcccgtcaa tgcggggaaa
      901 ctggtcctgg gatccccggc ctatgtgtcc gccaccccgc acggcatcat gctcctgctg
      961 gaacattata agattgaaac caggggcagg caggccgccg tgatcggcag aagcaatatt
     1021 gtcggccgcc ccatgagcat tctgctgagc gccaacagtt atcccggcaa cagcacggtc
     1081 accctctgcc attcccatac ccataacctg gaagagatct gccttcgcag cgatatcatc
     1141 gtggctgcgc tcggccgtcc cggttttgta aaagcagata tggtcaaaga aggcgcggtg
     1201 atcattgacg tgggcatcac ccgtgttccg gatgcatcaa aaaaaagcgg gtttgcctta
     1261 aagggagatg ttgatttccc ggccgtttcg ccaaaatgca gctatatcac accggttccc
     1321 ggcggggttg gacccatgac catcgccgcg ctgatgaaaa atactttttc cgcctgtgca
     1381 aacaggattt aaccggatat ctccatgaac cactccattg atatccccgt tcaggcactc
     1441 ccggtcatgg aagcttttta taccattcag ggagaaggct tccaccaggg caaagccgcc
     1501 tatttcatcc ggctggcggg ctgcgacgtg ggttgcgtat ggtgcgacgt gaaagagagc
     1561 tgggatgttt ccgcccatcc gctacgatcc gtcccggaga tcatttcaga agcaaagacc
     1621 caccccggaa ggatagccgt cgttaccggc ggcgaacccc tgatgcatga ttgcctgccc
     1681 ttaacccggc aattgaaggc cgccggattt cagacgcata tggaaacttc
//
LOCUS       DASYCU010000409         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_523138, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000409 DASYCU010000000
VERSION     DASYCU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523138"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VGM24_08265"
     CDS             complement(<1..678)
                     /locus_tag="VGM24_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEY4155408.1"
                     /translation="MKKFTPSNAWLKPDKICLLFIFCLVFCLGCRKSNVNNKDLRDFQ
                     QVNLVANSAAYAPALVDPTLQNAWGLAWAPSGIAWVNAQNDHVSELYTGEGVIVRPPV
                     NIPSPTDTIGGSPTGIVFSGGAGFTLSNKVAPNFLFIGDDGVLSGWNGAAGNNALRIG
                     NNPAPSSYTGLALATVSGRHYLYGANFGSRRIDVWDTTFTKVSMPFHDPAMPSGYSPF
                     NIQSVGSW"
     gene            complement(1031..>2765)
                     /locus_tag="VGM24_08270"
     CDS             complement(1031..>2765)
                     /locus_tag="VGM24_08270"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY4155409.1"
                     /translation="YVKGLQNQIETLPGEIETLKTKLISLPSGSDDTEKIRRQISKKE
                     DVLGVAKGDLIKWSRENYDRLTPESRNLYQKAFATNEADPDYHQLANLSYEEAGVKRN
                     LNIPKGDVLHKFRKDVNGGQLPAVSWLAGPENFSDHPTAPWYGAWYISEILDILTKNP
                     EVWKKTIFILTYDENDGYFDHIPPFAAPDPLNPGTGKCSAGIDTSVEYIRRDQELKHG
                     VGAKEARTGPTGLGFRVPMVIASPWSRGGHVCSQVFDHTSVFRFVQDFLNKKTGKRIR
                     ETNTSLWRKTVSGNLTAAFRPYHGEEQEKLPFLGKVPFIEAIYNAKFREAPSNYKVLP
                     AEEIARMNKDLFSSPHMPQQEPGIKPSCALPYQLYADGGLSDDKKNFHIKMEARNEIF
                     GEQSAGSPFNIYLPGKYDVDGKNVSFENTGSRSYAVIAGDGITDSWPVRSFENGLYHL
                     RVYGPNGFFREHQGNEKDPELLIQCEYERSPLTARKLTGELLVIIKNKDKHHSHQLEI
                     RDHAYKNKPVSAVVASGATIKVLMDQSKSYGWYDFGLTVKGFEHFEKRCAGRIETGQH
                     SFSDPYMGRMI"
BASE COUNT          687 a          684 c          647 g          747 t
ORIGIN      
        1 ccaggaaccg acggattgaa tattaaatgg tgaataaccg gatggcatcg cgggatcatg
       61 gaacggcatg gaaactttcg tgaacgtggt atcccaaaca tcaatccggc gggagccaaa
      121 attggctcca tacaaataat gcctgcctga taccgtagcc agcgccagtc cggtatagga
      181 tgaaggcgca ggattatttc cgatccggag tgcattattg ccggcagcgc cattccagcc
      241 ggacaaaacg ccgtcatccc cgataaaaag aaaattcggt gccaccttgt tggaaagggt
      301 aaaaccggca ccccccgaga aaacaatgcc ggtgggagat ccaccaatgg tatcggtcgg
      361 cgaaggtata ttgactggcg gtcttacgat gaccccttcg cccgtgtaca attcactcac
      421 atggtcgttc tgtgcattta cccatgcgat gccgctgggt gcccaggcca gaccccaggc
      481 attctgcagg gttggatcaa ccagggccgg tgcataggct gcactatttg ctaccaggtt
      541 cacctgttga aaatcacgca ggtccttgtt gttgacgttc gacttccggc atcccagaca
      601 aaagacaagg cagaaaataa aaagcagaca gatcttgtcc ggctttagcc aggcatttga
      661 aggagtaaac tttttcataa ttagcttgat tatttgggcg tgttaataaa taggcgatca
      721 ttccgcaatt acccggaagt aatcgccggt gaggaaaaat ggtgaatatg gaactccgtt
      781 gcttagggtg aggggttaat cagcgcgctt tcctttgctg cattccgggt ttggaatccg
      841 gggcaataag tatgaacggc gctttgtaaa gcagggttgc gtcctgcaga cgggaattat
      901 ataagatcag aaaatagaga gcgggaccag gtctttcttt tttgatgcgc catcagcgga
      961 tcatccaaaa cctcctttta cggcaatgtt gaaaatatca gcaaccggcg cgatctcagt
     1021 tagccgggat tcatatcatt ctgcccatat agggatcgct aaaactgtgc tgaccagttt
     1081 ctatccggcc ggcacaacgt ttttcaaaat gctcaaatcc ctttacggta agtccgaaat
     1141 cgtaccatcc gtaacttttg ctttgatcca ttagaacctt tattgtcgct cctgaagcaa
     1201 caactgcgga gaccggttta tttttatacg catgatccct gatctccagc tgatgagaat
     1261 gatgcttgtc tttatttttt atgatgacca gcagttcacc ggtgagcttg cgcgcagtca
     1321 acgggcttcg ttcatattca cattgaatca gcagttccgg atccttctca ttgccctggt
     1381 gttcccggaa aaagccattg ggaccgtaaa cccggagatg gtataatcca ttttcgaacg
     1441 accggaccgg ccaggaatcc gtaatcccgt cacccgcaat cacggcgtat gatctgctgc
     1501 ccgtattttc gaaagaaaca tttttcccat ccacatcata tttacccggg aggtagatat
     1561 taaacggcga tcctgcagac tgctctccga aaatttcatt ccgcgcttcc attttgatgt
     1621 gaaaattttt tttatcatcg cttagtccgc catctgcata cagttgatag ggcagcgcgc
     1681 aggaaggttt tattcccggt tcctgttgcg gcatgtgcgg ggaagaaaat agatctttgt
     1741 tcatccgcgc tatttcttca gcaggtagaa ctttataatt agaaggtgct tccctgaatt
     1801 tggcgttgta aatggcttca ataaaaggga ctttccccag gaagggaagt ttttcctgtt
     1861 cttcaccatg ataagggcga aaggccgcag ttaaattgcc gctcaccgtt ttccgccaca
     1921 ggctggtatt tgtttccctg atccgtttgc ccgttttttt atttaaaaaa tcctgcacga
     1981 aacggaaaac agaagtatga tcaaaaacct gggaacacac gtgccccccg cggctccacg
     2041 gtgacgcaat gaccatggga acacggaatc cgagaccggt agggcccgta cgggcttctt
     2101 tggcgcccac cccgtgtttt aattcctgat cacgccggat gtattcaaca gatgtatcga
     2161 tacccgcaga acattttccc gttcccgggt tcagcggatc cggtgctgca aaaggtggaa
     2221 tatggtcaaa gtatccatcg ttttcatcgt aggtaagaat aaaaatcgtt tttttccata
     2281 cctcggggtt tttagtgaga atatccagaa tttcggaaat ataccaggcc ccataccagg
     2341 gcgcggtggg atgatcagag aaattttcag gtcccgcaag ccaggacacg gccggaagct
     2401 gtccaccatt tacgtccttc cggaacttat gcagtacatc tcccttggga atattcaggt
     2461 tcctcttcac acccgcctct tcataactta aattggcaag ctgatgataa tcgggatccg
     2521 cttcgttcgt ggcaaaggcc ttctggtata aattcctgga ctccggtgtc aaccggtcat
     2581 aattctcccg gctccatttt atcagatctc cttttgctac gcccaataca tcctccttct
     2641 ttgaaatctg ccttcggatc ttttctgtgt catcgcttcc tgaaggcagg gaaatcagtt
     2701 ttgttttcaa tgtttcaatt tccccgggca gggtttcgat ctgattttgt aatcccttta
     2761 cataa
//
LOCUS       DASYCU010000410         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3567825, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000410 DASYCU010000000
VERSION     DASYCU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3567825"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2483
                     /locus_tag="VGM24_08275"
     CDS             <1..2483
                     /locus_tag="VGM24_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4155410.1"
                     /translation="LIIILIHEWHIFWPRTPMFSNYLLVAWRNLRKHRLNTVVILLGL
                     SVAFACSILLLLMVHHEFSYDAFHRNGSSLFEVYGLGHAPDGDEKTTSMSYPVAPTLK
                     SQVPGILRTTGWLEAGVGIRYKNKQIDKETKLVDNDFFSMFSFPVRSGKKLDPLSSTS
                     NVVLSQSTAEAVFGKEDPLGKVVKVNVNGDWRDLIVSAVLENAPENSSIRYDILARME
                     INGDYARLKDAWNSQNHIVFVQTAPGYSQQQVENGLRNIVKKHQVADEDYMKEKGYLK
                     DKNGDRYAWKLEPFASAHFDEALAFGPSISKTYLYILILIALVILTIASFNFINLNVA
                     RAFTRAREVGIRKTIGAGKKQIFFQFWAESFLLCCISVILGIVIAAALLRPFNALFTE
                     KLQITELIYPAVTAMVIGGMLTVSFLAGGYPAWLVSRFNPVNVLKGKVSMNRSSGLRS
                     GLITFQFILSCLLICSTIVIYKQFEFLRTAPLGFKQESVISIPVKNQQNARKYIEQLR
                     DRLYNQPQVVSISGSSANIGLGADYGISKWSWGFLYNGKNIETDMLKLDYDFFSTMGI
                     RPIAGREFNRSFPSDTSSSVSNVVITESLAKQLGKNNIVGLSFYADSAKPRWNVIGIV
                     PDIHFYSMYEKAAPFAFLMSSDGSLSYILVKVKTSNPLQAISLVKAAYKEIEPDNTLG
                     PDYLTENTRRWYAKEKRLSSIFFSAAAVAIILSCLGLFAIVSLVLEQRRKEIGVRKVL
                     GASLATITGLLSGDFLKLIALAFAIAVPIGWYFLNHWLQSYAYRAPLSWWIFPVAGLL
                     SIAIAMLTIGFQTLKASRVNPVESLRSE"
     gene            2535..>2982
                     /locus_tag="VGM24_08280"
     CDS             2535..>2982
                     /locus_tag="VGM24_08280"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4155411.1"
                     /translation="MRFTILFLAFLPAFAFAQIPYGNNPAAGHYCNTRGVKLYYEVYG
                     TGEPLLLAHGNGGSIENFKGIIPFFSQYYKVIAVDTRAHGKSADPGDSISFEMIADDF
                     NALLDSLHTGPVYFIGWSDGGIDGVGIAMRHPEKLKKMAITGTNIFV"
BASE COUNT          702 a          770 c          770 g          740 t
ORIGIN      
        1 tattgattat tatcctgatt catgaatggc atatcttttg gccccgaacg cctatgtttt
       61 ccaattacct gttagtcgcg tggaggaacc tccgcaagca ccggctcaat accgtggtta
      121 tcctgctcgg cctgtccgtc gcttttgcct gcagcatatt gctcctgctg atggtgcatc
      181 acgagttttc ttacgatgca tttcaccgga acggctctag cctttttgaa gtttacggtt
      241 tgggccatgc ccccgacgga gacgagaaaa ccaccagcat gagctatccg gtagcgccaa
      301 ccctgaaatc acaggtgccg ggtatattga gaaccaccgg ctggctggag gccggcgtcg
      361 gaatccggta caaaaacaag caaatcgata aggaaactaa actggtggat aacgatttct
      421 tcagcatgtt ttcctttccg gtgcgttcag gaaagaagct ggacccgctt tccagtacct
      481 ccaatgtggt cctgagccag tcaaccgcag aagcggtgtt cgggaaggag gatccgctcg
      541 gaaaagtggt gaaggtgaac gtgaatgggg actggcgcga tctgatcgtg tctgccgttt
      601 tagagaacgc gcctgaaaat tcttccatcc gttatgatat cctggcccgg atggagatca
      661 acggcgatta tgcccggctg aaagacgcct ggaacagcca aaaccatatc gtgttcgtcc
      721 aaacggctcc cggatattca cagcagcagg tggagaatgg tttgcggaat atcgtgaaga
      781 aacaccaggt ggctgatgag gactacatga aagaaaaggg ttatctcaag gataaaaacg
      841 gcgaccgcta cgcctggaaa ttagagccct tcgcctccgc gcattttgat gaagcgctcg
      901 ccttcggacc ttccatcagt aaaacctatc tctacatact gatattgatt gccctcgtca
      961 tcctgacgat cgccagcttt aatttcatca acctgaatgt ggcccgggct tttacgaggg
     1021 ccagggaagt gggcatccgc aaaaccatcg gtgcaggcaa aaagcagatc ttttttcagt
     1081 tctgggctga aagttttttg ctctgctgca tctccgtgat cctcggaatc gtgatcgcgg
     1141 ctgccttgct gcgcccgttc aatgccctgt ttacggagaa gttgcaaata accgaattaa
     1201 tatatcccgc ggtcaccgca atggtgatcg gaggcatgct gacggtatcc ttccttgccg
     1261 gcggttatcc tgcctggctg gtttcccggt tcaacccggt gaacgtactg aaaggaaaag
     1321 tttccatgaa ccgttccagt ggactgcgca gcggcctgat cacctttcag tttatccttt
     1381 cctgcctgct gatctgcagc accattgtaa tttataagca atttgaattt ctgaggacgg
     1441 caccgcttgg atttaagcag gaatcggtga tcagcatccc cgtgaagaac cagcaaaatg
     1501 cgcgcaaata tattgaacag ttaagagacc ggttatataa ccagccgcag gtggttagta
     1561 tatccggcag ttcggccaat atcggacttg gagcggatta cgggatcagc aaatggagct
     1621 gggggtttct ttacaacggc aagaacatag aaaccgatat gcttaaattg gattacgatt
     1681 ttttttccac catgggcatc cggcccattg ccggaaggga attcaaccgg tcttttcctt
     1741 ctgatacttc ttcctcggta tcgaacgtgg tgatcacaga aagcctggca aaacaactgg
     1801 gaaagaataa catcgtaggc ctgagttttt atgccgacag cgcgaagccc aggtggaatg
     1861 tcattgggat cgttcccgac attcattttt attcgatgta cgagaaagca gcacccttcg
     1921 ccttcctgat gagttcggat ggttccctct cctacatcct ggtaaaagta aaaaccagca
     1981 acccgttaca ggccattagc ctggtgaagg ctgcctataa agaaattgag ccggacaata
     2041 ccctgggccc cgactacctt acggaaaata cgcgtcgctg gtatgcaaag gagaaaaggc
     2101 tgtcctccat atttttctct gcggcggccg tggccatcat tttatcctgc ctcggactct
     2161 ttgccatcgt atcgcttgta ctggaacaac gacgcaagga aatcggcgtc cgcaaagtat
     2221 tgggggcttc cctggcgacg atcaccggcc tcctgtccgg agactttcta aagctcatcg
     2281 ccctggcttt tgccatagcc gtacccatcg gctggtattt tttgaaccac tggctgcaga
     2341 gctacgcgta ccgcgctccc ttaagctggt ggatattccc ggtagcggga ttgttatcca
     2401 tcgcgatagc catgctgacg atcggtttcc aaacgctgaa agcctcccgg gtaaacccgg
     2461 tggagagtct gcggtcggaa tgaggaatgg gttccggttt ccgtttgttt cttatcttca
     2521 aacaaactca acgcatgcga ttcaccatcc tgttcctggc tttccttcct gcttttgcct
     2581 ttgcccagat cccttatggg aacaaccctg cagcggggca ttactgcaac acgcgcggcg
     2641 tgaagctcta ctatgaagtg tatggaacag gcgaaccgct gctgctggcg catggcaatg
     2701 gcggatcgat cgaaaacttc aagggtatca tccctttttt ctcccaatat tacaaagtga
     2761 tcgcggtcga cacccgggcc cacggcaaat cggccgaccc cggcgactct attagtttcg
     2821 agatgatcgc agacgatttc aatgcgctgc tcgattcgct gcacacgggg ccggtgtatt
     2881 ttatcggctg gagcgacggc ggcatcgacg gggtggggat cgcgatgcgg cacccggaga
     2941 agctgaagaa gatggcgatt acgggaacaa atattttcgt ag
//
LOCUS       DASYCU010000411         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3134500, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000411 DASYCU010000000
VERSION     DASYCU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3134500"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VGM24_08285"
     CDS             complement(<1..1335)
                     /locus_tag="VGM24_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY4155412.1"
                     /translation="MPIRMTDDPEQPDDSGGGFEGGGGRGGGSGGGIFGLLPLILSLI
                     RGPKSFLLLLLIAAGAYFIFGRGGCNSAVVQQGIQQLATGGILDPRVFDRAKVYESLA
                     DDDSKNPLPESANLQRYAPAVGDQGKQGSCVAWSSAYGARTILEAARTGKAPDELKFS
                     PSFLYNQIGVDACDGSYINRAMEFMTRRGSVPYADFPYSEEDCKRQPDQQLLDEAQQY
                     RMRGANRLTAGDRTDQIDLRAIRENLSQGAPVVIGMMVGGTYMQAMMGKDVWNPTQDD
                     ESMMGFAGHAQCVVGYDDAKYGGAFLIMNSWGPQWGNNGFAWVRYKHFKYFVREAYGL
                     EAMPAVGEAANKPFEGEVGLVEMKNNGNKLVAGDYIKLKQTSGNVFETETPVKPGTRF
                     KMEVRNGSECFIYVFGKETDGSSYTLFPYPRTDDPSKTKYSPFCGIAGYRLFP"
     gene            complement(1432..2646)
                     /gene="lysA"
                     /locus_tag="VGM24_08290"
     CDS             complement(1432..2646)
                     /gene="lysA"
                     /locus_tag="VGM24_08290"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY4155413.1"
                     /translation="MIKPLPPETLIQLAEQYGTPLYVYHAEKIKDQFGRLNAAFGKSD
                     VVFFYACKALTNINILRYVKNIGANVDCSSINEVKLALHAGFPASRILYTSNGIGFSE
                     IEEARELGVIINIDSLSNLDKFGKKYGHSYPVGIRLRPNILAGGNLKISTGHDKSKFG
                     IPVDQIGQILEVVKKNNMLIRDLHIHTGSEIQDVDVFIKGIEILFEITHYFPELEFID
                     LGGGFKIVSRPGDKETDIALLSEKLESAFANHPYQNGKRLQIWFEPGKFMVSESGFFV
                     TRVNVMKETSAAAFVSVDSGFNHLIRPMFYDAYHPIENISNPQGPVKNYSVVGNICET
                     DTFAWNRPLNEVREGDYLVFRNAGAYGFEMSSNFNSRLKPAEVMVLEGKAHLIRKRDE
                     FADLLRHQVEVI"
     gene            complement(2662..3375)
                     /gene="scpB"
                     /locus_tag="VGM24_08295"
     CDS             complement(2662..3375)
                     /gene="scpB"
                     /locus_tag="VGM24_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY4155414.1"
                     /translation="MEISHLIPHIEALIFASDKPLTAIEMTELLNNAFGFMEDKLTLE
                     QVQTGIEGIVSKYNSDFYPFEVRESGGGWQFLSKKEFHKTIAQLNGEKFLKRLSTAAL
                     ETLAIVAYKQPITKGEIESIRGVSCDYSIQKLLEKELIIITGRNEEMPGKPLIYTTSR
                     SFMDYFGINQSADLPKLKEVFGESLQEPSLVNYTPAAPEINPAFVVSEEGELLDKSGS
                     ENEENPFPGESGTPGSSDQ"
     gene            complement(3421..4302)
                     /gene="atpG"
                     /locus_tag="VGM24_08300"
     CDS             complement(3421..4302)
                     /gene="atpG"
                     /locus_tag="VGM24_08300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY4155415.1"
                     /translation="MAGQLKEVRNRIKSIQSTQQITKAMKMISATKLRKAQEAIQQMR
                     PYSKKLQQVLSNIVSDSDGAGRSGLSTPRPVEKALLIVITSDRSSCGAYNSNLIKLAK
                     ATLRDKYPAQAAKGNVMIWSIGKKGYEHFLKNNYPVNGEFKDIFQNLRFENVQTCAQA
                     AVRAFENREFDAVDIIYSEFKNASTQRFVVEPFLPIPKMEMAPGTRTNDFIFEPSRDE
                     LINELMPKILNTQLFKAVLDANASEHGARMTAMDKATENANEILRTLRISYNRARQAA
                     ITTELTEIVSGAAALQG"
     gene            4440..>4803
                     /locus_tag="VGM24_08305"
     CDS             4440..>4803
                     /locus_tag="VGM24_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY4155416.1"
                     /translation="MPKTIKKDRPVILITNDDGVMAPGILNLVEAVKDLGKVVVVAPD
                     KPQSGMGHAITIGLPLRLHPVDIFEGVEAWQCSGTPVDCVKLAVDKVLRRKPDICLSG
                     INHGANHSINVIYSGTMSA"
BASE COUNT         1066 a         1285 c         1230 g         1222 t
ORIGIN      
        1 tggaaacaaa cggtaaccgg cgatgccgca gaaaggagaa tatttggttt tggaaggatc
       61 gtccgttctg ggatacggaa ataaggtata actgctgccg tcggtttcct ttccaaacac
      121 atagataaaa cattccgagc cgttccggac ttccatcttg aaccgggtcc cgggttttac
      181 cggggtctcc gtctcaaata catttccgga ggtctgtttc aacttgatat aatccccggc
      241 aaccagtttg ttcccgttat ttttcatttc caccagcccc acttctcctt caaagggttt
      301 gtttgccgct tccccgaccg ctggcatggc ttccagtccg taggcttccc ggacaaaata
      361 tttaaaatgc ttataccgca cccaggcgaa cccgttattg ccccattggg ggccccagct
      421 gttcatgatc agaaaagcgc ctccgtattt ggcatcgtca tagcccacca cgcattgtgc
      481 gtgcccggca aaacccatca tgctttcatc gtcctgcgtc ggattccaca catccttccc
      541 catcatcgcc tgcatatagg taccgcccac catcatgccg atcacgacgg gagctccctg
      601 cgaaagattt tcccggatgg cgcgcagatc gatctgatcc gtccggtcgc cggcggtgag
      661 ccggttggcg ccgcgcatcc ggtattgctg ggcttcatcc agcaactgct ggtccggctg
      721 acgtttacaa tcctcttcgg aatagggaaa atccgcgtaa ggaacagatc cgcgccgggt
      781 catgaattcc atggcgcggt tgatatacga tccgtcacag gcatccaccc cgatctggtt
      841 gtataagaaa gaagggctga attttaattc atcgggcgct ttcccggtgc gggccgcttc
      901 gagaatggtg cgggccccgt aagcactgct ccaggccacg cagctgccct gttttccctg
      961 gtcgcccacc gccggcgcat aacgttgcag gttagccgat tccggcaggg gattcttgct
     1021 gtcatcatct gcgagggatt cgtatacttt tgcccggtca aaaacgcggg gatcgaggat
     1081 gccgcccgtg gcgagctgtt ggattccctg ctgcacgaca gccgaattgc atcctccgcg
     1141 gccaaaaata aaataagctc cggcagcgat cagcagcaac aggagaaaac ttttgggacc
     1201 gcggatcagg gaaaggatca gcggcagcag gccgaatatt ccgcccccgg atccgccgcc
     1261 ccttcctccg ccgccttcaa aaccaccccc gctatcgtcg ggctgttccg gatcatctgt
     1321 catgcgaata ggcataccca aaagtttaaa ggcttaataa tataccgctt tcccgcaggc
     1381 ccgaaagcag acgctatttg aaaataaaga aagcggggag aattaaaaaa tctaaatcac
     1441 ctctacctga tggcggagca ggtctgcaaa ctcatcccgt ttgcggatca ggtgtgcctt
     1501 cccttccagc accatgactt cggccggttt gagacgggag ttaaaattgg aagacatttc
     1561 aaaaccgtag gcgccggcgt tccggaagac caggtagtct ccttcccgca cttcgttcag
     1621 cggccggttc caggcaaagg tgtcggtctc gcatatattt ccgacaaccg aataattttt
     1681 gacaggcccc tgcgggttgc tgatgttttc tatcggatga tacgcatcgt agaacatggg
     1741 acggatcaag tggttgaagc cgctgtccac gcttacaaaa gctgccgcgc tggtttcctt
     1801 catcacattt acgcgggtga caaaaaatcc ggattcgctt accatgaatt tccccggttc
     1861 aaaccagatc tgcaggcgtt ttccgttttg gtaaggatgg tttgcaaatg ccgattccag
     1921 tttctccgac agcagggcaa tatcggtttc cttatcgccg ggccggctga cgatcttaaa
     1981 acctcctccc agatcgatga attccagctc ggggaaatag tgagtgatct caaacaatat
     2041 ttctatccct tttatgaata catccacgtc ctggatctcg ctgccggtat gaatatggag
     2101 atcccggata agcatattgt tttttttgac gacttccagg atctgaccga tctggtccac
     2161 cggaataccg aacttgctct tgtcgtggcc ggtggagatc ttcaggttgc ccccggcgag
     2221 gatattcggc cgcaggcgaa tgcccacggg gtaactatgc ccgtatttct taccgaattt
     2281 atccaggttg gaaaggctgt cgatattgat gataacgccc agttcccgcg cttcttcgat
     2341 ttcgctgaag ccgatgccgt tggaagtata caggatccgc gaggccggga aacccgcgtg
     2401 cagggccagc ttcacttcat taatggaact gcagtccaca ttggccccga tattttttac
     2461 ataacgcagg atattaatat tggtcagcgc cttgcaggca taaaagaaga ccacatccga
     2521 tttgccgaac gccgcattca gccggccgaa ctggtctttg atcttttcag catgataaac
     2581 atagagtggc gttccgtact gttcagcgag ttgaataagt gtttcgggcg gcaggggttt
     2641 aatcatgaat gcgaatatat cttattgatc tgatgaaccg ggtgttccgg attcgccggg
     2701 gaaggggttt tcttcatttt cagaaccgct tttatccagc agttctcctt cttcactcac
     2761 cacgaacgcc ggattgatct ccggagcagc cggcgtatag ttgaccaggg agggttcctg
     2821 caaactttct ccaaatactt ccttcagctt gggaagatcc gcagactggt tgatgccgaa
     2881 ataatccatg aaactccggg acgtggtata gatgagaggc ttgcccggca tttcttcatt
     2941 ccgcccggta atgatgatca gttccttttc cagcagtttc tgaatggaat aatcgcagct
     3001 caccccgcgg atggattcta tctcgccctt ggtgatgggt tgtttatagg ccacgatggc
     3061 cagggtctcc agggcagcgg tcgacaagcg tttcaggaat ttttccccgt tgagctgggc
     3121 aatggtcttg tgaaactcct tcttggataa aaactgccat ccgccgccgc tttcccgcac
     3181 ttcgaaggga taaaagtccg agttgtattt ggaaacgatc ccctcaatgc ccgtctgaac
     3241 ctgttccagc gtgagcttgt cttccatgaa tccgaacgca ttgttcagga gttcggtcat
     3301 ttctattgcc gtcagcggtt tgtcgctcgc aaagatcagc gcctcgatat gtgggatgag
     3361 atgggagatt tccatgggat caatttaata cttttcaaaa cagcacttac cggcctgatc
     3421 ttacccctgc agggctgctg ccccgctgac gatttccgtc agctctgtgg tgatggctgc
     3481 ctggcgggcc cggttatagg aaatgcgcag cgtgcgcagg atctcgttgg cattttccgt
     3541 agccttatcc atcgccgtca tccgcgcgcc atgttccgaa gcattggcat ccagcacagc
     3601 cttgaacagc tgggtgttca ggatcttcgg catcagttcg tttatcagtt catcccggga
     3661 gggttcaaaa ataaaatcat tggttcttgt tccgggcgcc atttccattt taggaatggg
     3721 caggaaaggt tccaccacaa aacgctgggt gcttgcattt ttaaattcac tgtagatgat
     3781 atccacggca tcgaattccc ggttctcaaa tgctcttacg gcagcctgcg cgcaggtttg
     3841 gacgttctcg aagcggaggt tctgaaatat atctttaaat tccccgttta ccgggtagtt
     3901 attcttgaga aagtgttcgt atcccttttt tccaatgctc cagatcatca cattgccttt
     3961 ggcagcctgc gcaggatact tatcccgcag ggttgctttt gccagtttga tcaggtttga
     4021 attgtaggcg ccgcaggagg aacggtcgct ggtgatcacg atcagcagag ctttttcgac
     4081 cggccgggga gtggaaagac ccgagcggcc ggcgccgtcg gaatcactga cgatattgct
     4141 caatacctgc tgcagctttt tggaataggg acgcatctgc tggatagctt cctgggcttt
     4201 ccgcagcttg gtggcgctga tcattttcat cgccttggtg atctgctggg tgctttggat
     4261 ggatttgatc cggttgcgaa cttccttgag ttgtcctgcc atttgaggta ttttaaaccg
     4321 gctgcaaagg tagcgtaagc ccctcaaaat gcaagtagac tgtggaaaaa agaagccttt
     4381 ccgccctacg ggagcgtgcg ggaaggaaaa aatagtactt tcgctcccct gaattcatta
     4441 tgccaaaaac aattaagaaa gaccggcccg ttatcctgat caccaacgac gatggggtaa
     4501 tggctcccgg gatcctcaac ctcgtagaag ccgttaaaga tcttggaaaa gtggtggttg
     4561 tggcgcccga taagccgcaa tcgggcatgg gacatgccat caccatcggc ctgccgcttc
     4621 gcctccatcc ggtggatata tttgaagggg tggaagcctg gcagtgctcc ggaacacccg
     4681 tggattgcgt aaaactggct gtggataagg tccttcgccg gaaacccgat atctgcctca
     4741 gcggtatcaa ccacggggca aaccattcca tcaacgtgat ctattccgga accatgtccg
     4801 ccg
//
LOCUS       DASYCU010000412         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2407156, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000412 DASYCU010000000
VERSION     DASYCU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407156"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGM24_08310"
     CDS             <1..217
                     /locus_tag="VGM24_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155417.1"
                     /translation="WINFLFLVLIGFETASCKQNSTKSVTEDTTVNQAIPPPPDNSQA
                     TNPSLADTAYPKKDTMITKRDSSASRK"
     gene            complement(315..1541)
                     /locus_tag="VGM24_08315"
     CDS             complement(315..1541)
                     /locus_tag="VGM24_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY4155418.1"
                     /translation="MSDQDSTPDIPAVLEKFHAEIAGELDHILDYWSRFSIDGEKGGF
                     VGKVDNGNNQIPAAAKGAVLNTRILWTFSAAYPLTGNPAHLAIADRAFCYIRDHFTDT
                     RHGGLFWSLDADGHALDTHKQVYAQSFGIYGMSEYYRSTRNEQALALAIEWYRLIEQY
                     SLDTRNGGYTDACSRDWSFLQDKRLSAKDDNAVKTMNTHLHVIEGYANLYEIWPDPLL
                     RIRIQELLALFREKIQNKKNNHLELFFDEEWNLKPGIISYGHDIEAGWLLQHCAESIN
                     DETWILFAKANAVRITDAAMEGLDEDGGLWYERDTKNRKLVKEKHWWPQAEALIGLCN
                     AWQISGSTRYREAMLKNWQFIKKYIRDPEKGEWFWGIRHDHSIMEDQDKIGLWKCPYH
                     NGRACMQIVKRLSPHR"
     gene            complement(1555..>1946)
                     /locus_tag="VGM24_08320"
     CDS             complement(1555..>1946)
                     /locus_tag="VGM24_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEY4155419.1"
                     /translation="WLHLAHGVRNTAAGLRYVLYIFMTDLEDLTKVIHKPAGYFLAPD
                     GEERMGDVSNVVFCNGWIWNEDGTVFIYYASSDTRMHVAVTSVERLLDYTLNTAPDGF
                     TSAHSVQQINKLIEKNHEVLHKEKVKV"
BASE COUNT          496 a          506 c          414 g          530 t
ORIGIN      
        1 atggatcaat tttctctttc tcgtcctgat cggatttgag acggcctcct gtaaacagaa
       61 ttccacaaaa tcggtaacag aagacacaac cgtaaaccag gcaattcccc cgccgcccga
      121 caatagccag gctaccaatc catcccttgc ggatactgcc tacccgaaaa aagataccat
      181 gatcacaaaa agggacagct cagcatcgag aaaataaacc aatattcaaa cgaggaattt
      241 gggatttttt tgaggcattc ccggaaatcc gcatttgttt atacccggat gccggtcaac
      301 cggaataaac cggattaccg atggggagat aatcttttta ctatttgcat gcaagcccgg
      361 ccgttgtgat aagggcattt ccacaaaccg attttgtcct ggtcttccat tatggaatgg
      421 tcatgacgga tgccccagaa ccattcccct ttttcaggat cccggatata ttttttgatg
      481 aactgccagt tctttagcat ggcctcccgg taacgggtac tcccggaaat ctgccaggca
      541 ttgcaaagtc cgatcagtgc ttcagcctgt ggccaccagt gcttttcttt cacaagtttc
      601 ctgttttttg tatcccgttc ataccatagt ccgccatctt catccagccc ttccatagcc
      661 gcatctgtta tgcgaaccgc atttgccttt gcaaagagaa tccaggtttc gtcatttatg
      721 gattccgcgc agtgctgcaa taaccacccg gcttcaatgt catgaccgta ggaaataatt
      781 cccggcttca ggttccattc ctcatcaaaa aaaagttcca ggtgattatt tttcttattc
      841 tgaatctttt cccggaaaag tgcaagcagt tcctgaatgc ggatccggag gaggggatcg
      901 ggccatatct catacaggtt tgcatatcct tcgataacat gcaggtgagt gttcattgtc
      961 tttaccgcat tatcgtcttt tgcgctcagc cttttatcct gcagaaaaga ccagtccctg
     1021 gaacatgcat cggtataacc tccgttgcgg gtatccaggc tatattgttc aatcagcctg
     1081 taccattcaa tggccagggc cagggcctgt tcgttccggg tactgcggta atattcactc
     1141 attccatata ttccaaaaga ttgggcgtag acctgtttat gggtatccag tgcatgccca
     1201 tctgcatcga gcgaccagaa taaacctcca tgccgagtat ccgtaaaatg atcccggata
     1261 tagcaaaatg ctctatccgc aattgcaaga tgtgccggat tgccggtcag gggataagcc
     1321 gctgaaaagg tccagagtat gcgggtattc agcacggcac cttttgcggc agcgggaatc
     1381 tgattattcc cgttatctac tttccccaca aatccgcctt tttccccgtc aatggaaaac
     1441 cggctccagt agtcgagtat atgatccaat tccccggcga tctccgcgtg aaatttctcc
     1501 aaaaccgcag gaatgtcggg agtgctgtcc tggtcgctca taaataaccc gggattaaac
     1561 tttcactttt tccttatgga gaacttcatg attcttttca atcagtttgt tgatctgttg
     1621 aaccgaatga gccgaagtaa atccgtccgg cgcggtgttc agcgtgtaat ccagcaatct
     1681 ttcaacggaa gtaacggcca catgcatacg ggtatcggac gaagcataat agataaaaac
     1741 cgttccgtct tcattccaga tccatccgtt gcaaaaaacc acattcgaca cgtctcccat
     1801 ccgttcttct ccgtcaggag cgagaaaata tccggccggt ttgtgaatta cttttgtcaa
     1861 atcttctaaa tcggtcatga aaatgtacaa tacatatcgc agaccggctg ctgtatttct
     1921 gaccccatgg gccaggtgaa gccagc
//
LOCUS       DASYCU010000413         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_234169, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000413 DASYCU010000000
VERSION     DASYCU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234169"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VGM24_08325"
     CDS             <1..530
                     /locus_tag="VGM24_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY4155420.1"
                     /translation="SSADVEALAMQVEDSGGVSFVPAFAGLGAPYWNPDARGTIVGIT
                     RGTMAAHIARAAIESIALQTRDVLKAMQADAGLPIKELRVDGGATANNLLMQFQADLL
                     NCKVIRPEITETTALGAAYFAGLAVGFWESPESLQKLWKSEREFLPSVDPQEVKEKIA
                     GWERAVQAAQAWSKI"
     gene            564..1292
                     /locus_tag="VGM24_08330"
     CDS             564..1292
                     /locus_tag="VGM24_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY4155421.1"
                     /translation="MSPFLAEYIGTFMLILLGNGVVANVVLTDTKGHNGGWIVITTGW
                     GLAVYVAVVIAGPVSGAHLNPAVTLALALAGKFAWANVLSYLLAQMLGAMSGALLVWL
                     IYRDHFNRTKDPGLQLASFCTAPAIYNPVTNIISEAAGTFVLTYTVFYITGAEITSSK
                     TLIGLGSVGALPVALLVWVIGLSLGGNTGYAINPARDLAPRIMHAILPMNGKGKSNWS
                     YAWIPVLGPLLGGLIGALVYLSMR"
     gene            1449..2549
                     /locus_tag="VGM24_08335"
     CDS             1449..2549
                     /locus_tag="VGM24_08335"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEY4155422.1"
                     /translation="MFNPVKTPGTLFLGLLFFCSISNTRGQGVITTPRTPSPAATVSQ
                     TIGISTVSVSYSRPSVRGREVWGKLVPYGWNIDGFGAGNPAPWRAGANENTVLRLSHP
                     ATVAGHAVPAGEYGLFFTINKDNSTGEVVLSKDYKSWGSFWYDPKLDEMREKISLRDG
                     PPTELLTYDFENITRTGAELVLSWEKKQFPVKIEFAVDDIVMANAAEELKNTVGFSWQ
                     GYASAANYSLMNKVNPEQGLKWADLAVQQNNSFTTLSIRSGLLKQLGRTDESEKVMKD
                     AVALATEVELNNYGYQLLNQQEADKAIQIFVLNTQQHPKSANVWDSLGEGYAIKGDKK
                     NAILNFKKALSLNPAPNVKANSEKYLKQLGEM"
     gene            complement(2632..3078)
                     /locus_tag="VGM24_08340"
     CDS             complement(2632..3078)
                     /locus_tag="VGM24_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155423.1"
                     /translation="MKKRIMIIALVLVSITGFSFAADVPSVNNQVLSSFNREFVNARD
                     VKWESHKNFVKASFRLNEMTLYAYYLPNGDQLGVTRFISPNQLPIQLLTSLKKDFSAY
                     WVSDLFEVSGDSGTSYYITIENDSQVKVMVSSDSIDWKTYKVTDKE"
     gene            complement(3466..>3813)
                     /locus_tag="VGM24_08345"
     CDS             complement(3466..>3813)
                     /locus_tag="VGM24_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY4155424.1"
                     /translation="MINIRSLRGNNRLILVFPLVALFSCSRAPEKHIPKTDTVVIAAM
                     KFQPAVLTLHKGDSVVWINKDMVAHDITQVPSNTWTSSSLPPGKSWKMAVEESDDYYC
                     SIHIVMVGKLIVK"
BASE COUNT          961 a          901 c         1006 g          945 t
ORIGIN      
        1 ggtcctctgc agacgtggag gcgcttgcca tgcaggtgga ggacagcggg ggagtctctt
       61 ttgtccccgc gtttgcgggc ctgggtgcgc cttactggaa tcccgatgcc cggggaacga
      121 ttgtcgggat cacccggggc acgatggcgg cgcatattgc gcgcgccgcg attgaatcca
      181 ttgccctgca aacccgcgat gtactgaaag cgatgcaggc ggatgccggc ctgcccataa
      241 aagaattgcg ggtggacgga ggggctacgg caaacaacct gctcatgcag tttcaggccg
      301 acctgctcaa ttgcaaagtg atccggccgg agattaccga aacgactgcc ctgggggcag
      361 cttattttgc cggattggcc gtcggttttt gggaaagccc ggagagcctg caaaaattat
      421 ggaagtctga aagagaattt ctgccttcgg ttgacccgca ggaagtgaaa gaaaaaattg
      481 ccggatggga gagggccgtt caggcggcac aagcctggag taaaatataa gatccattaa
      541 aaacacttaa aattcaaacc gacatgagtc catttttagc tgagtacatc ggaactttta
      601 tgctgatctt attgggaaac ggagtggttg ccaacgtggt tttaacagac acaaaaggcc
      661 ataacggcgg ctggatcgtc attaccaccg gatggggact ggccgtatac gtagccgtag
      721 tgatcgccgg cccggtgagc ggggcgcatc ttaatccggc ggtcaccctc gctctggctt
      781 tggcaggaaa atttgcctgg gcaaatgtct tgtcttacct gttggcccag atgctgggcg
      841 ccatgtccgg ggcattactg gtctggctga tataccggga tcattttaac cgcacgaaag
      901 atccgggatt acaactggct tctttctgta cggcgcctgc catttacaac ccggtcacca
      961 atatcatcag cgaggcagcg ggaacctttg ttctcaccta tactgtattt tatattacgg
     1021 gtgcagagat cacctcgagc aaaaccctga tcggattggg ttcggtgggc gcattaccgg
     1081 tggccctgct ggtatgggtg atcgggttgt ccctcggcgg gaacaccggt tacgccatca
     1141 atcccgcccg ggacctggcg ccgcgcatta tgcacgccat tctgcccatg aacggcaagg
     1201 ggaaaagtaa ttggtcttat gcctggatcc cggtgcttgg gcccctgctt ggcggtttga
     1261 tcggcgcttt ggtttatctt tccatgagat gatccgaaat gccggcggcc gatggcggac
     1321 accgtttgcc cttcattttt catcattccg gccagcacaa tgaccgttgg aacattactg
     1381 tatttggatc gcccgtaaaa gtgctatctt aatacaagcc ttcctattat tcaccaaaac
     1441 ttcttcaaat gtttaacccg gtaaaaacac caggaactct tttcctcggc cttttatttt
     1501 tttgcagtat ttccaatacc cggggccagg gggttatcac cacgccgaga acgcccagcc
     1561 cggcagctac cgtttcgcaa acgattggaa tttccactgt ttcggtcagt tattcaaggc
     1621 catccgtgag aggccgcgag gtctggggaa agctggttcc ctatggatgg aatatagatg
     1681 ggttcggagc tggaaatccg gccccctggc gtgccggtgc caatgaaaac acggtgctcc
     1741 ggttatcgca tccggcaacc gttgcaggac atgccgtgcc ggccggggaa tacggattat
     1801 tttttaccat taacaaagac aattctaccg gagaagttgt tttgtcaaag gattacaagt
     1861 cctggggcag tttctggtac gatcccaaac tggatgaaat gcgggagaag atcagtttgc
     1921 gggacggccc gcccaccgaa ttgctgacct atgatttcga aaatatcacc cggaccgggg
     1981 ccgaactcgt tctgagctgg gagaaaaaac aattcccggt gaagatcgaa tttgctgtgg
     2041 acgatattgt catggccaat gcagcggagg aattaaaaaa cacagttggc ttttcctggc
     2101 agggctatgc cagcgcagcc aactattcgc ttatgaacaa ggtgaatccc gagcagggac
     2161 tcaaatgggc cgacctggcc gttcagcaaa acaattcttt tacgacttta agcatccggt
     2221 ccggcctcct gaaacaactg gggagaacgg atgaaagcga aaaagtaatg aaagacgccg
     2281 ttgccctggc aacggaagtg gaactcaata attacggata ccagttgctg aatcagcagg
     2341 aggcagacaa agccattcag atattcgtat taaatacgca gcagcatccc aagagcgcca
     2401 atgtctggga cagcctgggg gaaggatatg ccatcaaagg cgataagaaa aatgccatcc
     2461 tcaatttcaa aaaagcgttg agcctgaatc ccgcgcccaa cgtgaaagcc aattcggaaa
     2521 aatacctcaa acaactgggt gaaatgtaga ccgcattcaa aggggcaatc aaaaaaaaaa
     2581 tccactgatc agtggatttt tttttgcagg atgcgattgc gggtgaatac attattcttt
     2641 gtcagtcacc ttataggtct tccagtcaat ggaatcggaa gataccatca cttttacctg
     2701 tgaatcgttt tcgatggtga tgtagtagga agtgccgcta tccccgctca cttcaaacag
     2761 atcgctcacc caatacgctg aaaaatcctt tttcagtgag gttaaaagct ggataggcag
     2821 ctggttggga ctgataaacc gggttactcc cagctggtcg ccgtttggta aataataggc
     2881 atacagggtc atttcgttca gccggaagga agccttcacg aaattcttat ggctttccca
     2941 ttttacatcc cgggcattta cgaattcccg gttaaagctg cttaatacct ggttgtttac
     3001 ggacggaacg tctgcggcga agctaaagcc ggtgatactt acaagtacca gggctatgat
     3061 catgattctc tttttcattg tcttattttt taaaagtgtt tattgactta atgacaatgc
     3121 aaagataagg gttaaagtat gtactatgca atacatagta atatgaatgg cataataata
     3181 ggtataggcg gtaaaattgt catttttccg ggccgaaatt cctaatattc tgatagaaag
     3241 ggggttataa ggctggtttt gtaccgttcc ggacaggccg aaattggcat aattagattc
     3301 aaggccaatt tatgggttct cccgggatgg agccgggcgg ctgattcggt aagttttgtc
     3361 cctggttcct ggcttttggc caggaaggat atgcccgggt taaccggtgc gggagtaaaa
     3421 ggaaagggac aaaatgaacg atcctatccc tttccaggct gagtttcact tcacgatcag
     3481 tttgccgacc attacgatgt gaatactgca gtaataatca tcgctttcct ctacggccat
     3541 tttccatgac ttcccgggag ggaggctgga agaagtccag gtattgctgg gcacctgggt
     3601 tatatcgtgt gccaccatat ccttattgat ccataccacg ctatctcctt tatgaagggt
     3661 cagcacggca ggctgaaatt tcattgctgc aataacaacg gtatcggttt tcgggatatg
     3721 cttttccgga gcacgggaac aactgaacaa cgcaaccagg ggaaacacca ggatcaggcg
     3781 gttatttccc ctaaggcttc ggatgtttat cat
//
LOCUS       DASYCU010000414         4928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3568708, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000414 DASYCU010000000
VERSION     DASYCU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3568708"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGM24_08350"
     CDS             <1..344
                     /locus_tag="VGM24_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155425.1"
                     /translation="AAGSGSNRHKAGGRMRRNDSSSAKVNDTAVAIIPQRATVWVVDK
                     DSLIRKKIVVGLNDDTHVEVLHGLSEEDEVVDAVQDPALQPAASAGPAKSPFLPQRRR
                     NSNPKKPPQPR"
     gene            373..1128
                     /locus_tag="VGM24_08355"
     CDS             373..1128
                     /locus_tag="VGM24_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4155426.1"
                     /translation="MSKILEIQNIRREFRMGTETVRALKGVSFDVNAGEFVTIMGSSG
                     SGKTTLLNILGCLDKPSDGHYLLDGVNIGGLERNELAHLRNIKIGFVFQAYNLLPRTS
                     ALENVELPLLYNPTIPAKERKERAVHALESVKLAERLDHTPNQLSGGQQQRVAIARAL
                     VNEPVMILADEATGNLDSRTSYEIMALIQELNQQQGKTIVFVTHEPDIASFSSRTITL
                     KDGKIIKDSANENRRSAKEVLAALPAVDDYQNE"
     gene            1147..2367
                     /locus_tag="VGM24_08360"
     CDS             1147..2367
                     /locus_tag="VGM24_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4155427.1"
                     /translation="MNVLNLVRIASKALQRNKLRAFLTMLGIIIGVGAVIAMVAIGQG
                     SKKSIQDQLSSMGSNMITIRPNSNVVAGARLDAGSVQTLKIEDVKAIQKQAQYINGIS
                     PAASARGQAINGALNWPTSIQGVSPEFLDIRAWTVKDGIAFTDADVTAAAKVCLLGQT
                     VVSNLFVNGENPIGKIIRFNKIPFRVIGVLGQKGENAFGQDQDDIILTPYTTVQERIL
                     STIYFQNIYVSAKNEQVTDAATAEISQILRVTHRLKPTDIDDFAVRTQAELINTFSST
                     SQLLTVLLTAIAGISLVIGGIGIMNIMYVSVTERTREIGLRMAIGARGIDILLQFLIE
                     AILISITGGLIGVGLGIIASRLVTQFLSWPTLISESSIVLSFLVCAFTGVFFGYYPAQ
                     KASRLDPIEALRYE"
     gene            2501..3043
                     /locus_tag="VGM24_08365"
     CDS             2501..3043
                     /locus_tag="VGM24_08365"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY4155428.1"
                     /translation="MKNLLLIGVTTLILSCNNNGGTATNPASDSVAAGAASKDSVLEK
                     NKATALASEEAFNSGKLDDAFKDAVPDAVDYGDGSMAPMKGLDSIKAGVKIFLNAFPD
                     YKGENFLAIGEGNHVAVFGDWSGTFKNPLMGMKPTGKSFKIKDVDLFTFNDQGKITEH
                     RSVQSSKTVLDMVQAKMMKK"
     gene            complement(3177..3467)
                     /locus_tag="VGM24_08370"
     CDS             complement(3177..3467)
                     /locus_tag="VGM24_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155429.1"
                     /translation="MNNTLQSVQDSLRSESPPDTFSVFLKALWYDRKGEWDKAHGLVD
                     HLEGREAARVHAYLHRKEGDISNAGYWYRQAGTEQPDLSLEKEWEILVRQFV"
     gene            3560..4627
                     /locus_tag="VGM24_08375"
     CDS             3560..4627
                     /locus_tag="VGM24_08375"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY4155430.1"
                     /translation="MDILNQRLVNQQIAVHRFHLPEELVCWQGAVQAQDYAMAKWAIG
                     CRLDGITETQVEKDFNEGKILRTHVLRPTWHFVSPKDIRWMLALTAPKIKTFTKGHHR
                     DLGIDSGILKRSKKIIEKLLKGEGPKTRKEIEQALNKSRIGTDNNRLGFLLIDAELDG
                     LLCSAGRKGKQFAYGLLEEIVPEFKTRKREEGIVELAGRYFGSHGPATVHDFAWWSGL
                     SLSEIKRILPGMGSGLLSQKMDGQDYWFSRSMAEAKGGPKGIYFLPAFDEYFIGYRDR
                     THSMDTSFAKRIMGPNGIFNPVIVRKGRIAGTWKRKEEKSGSMIEAAPFASFKLSDRG
                     VASAMKKYSRYLGRAVSLAQF"
     gene            complement(4618..>4928)
                     /locus_tag="VGM24_08380"
     CDS             complement(4618..>4928)
                     /locus_tag="VGM24_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY4155431.1"
                     /translation="NLFDKYPNLYADIAARFAEIAPVPRYASAFITRYQDRLVYGTDM
                     GTAANMYRTTFRILETADEHFYETGMFDYHWPLYGLDLSKNILQKLYETNAAKIINQN
                     "
BASE COUNT         1293 a         1182 c         1335 g         1118 t
ORIGIN      
        1 cggccgccgg ctccggttca aaccggcaca aagcgggcgg ccggatgcgc cggaacgatt
       61 cttcatcagc aaaagtaaat gacacggcgg tagcgatcat tccacaaagg gcaaccgtat
      121 gggtagtgga caaagacagc ctgatccgga aaaagatcgt cgttggttta aatgatgaca
      181 cgcatgtgga agtgttgcat ggtttgtcgg aggaggacga agtggtggat gccgttcagg
      241 acccggccct gcagccggcg gcttcggcgg gaccggccaa gagtccgttc ctgccgcaga
      301 gacggagaaa ttcgaaccca aaaaaaccgc cgcagccaag ataaccggaa gaaggactaa
      361 aaagattcgg taatgagtaa aattctggaa atacaaaata tcaggcggga gttcaggatg
      421 ggcacggaaa ccgtacgcgc gttaaaagga gtttcattcg atgtgaatgc aggagagttt
      481 gtaaccatca tgggaagcag cggttcaggt aaaaccacct tattgaatat cctgggttgc
      541 ctggataaac cgtcggatgg acattattta ctggacggag tgaatatcgg cggcctggaa
      601 cggaacgagc tggcgcattt gaggaatata aaaatcggat ttgtgttcca ggcttacaac
      661 ctgctgcccc ggacttctgc gctggaaaat gtggaactgc ccttattgta caatccgacc
      721 attccggcaa aggagagaaa agaaagagcc gtgcatgcgc tggaatcggt gaagctcgcg
      781 gaacggcttg atcatacacc gaatcaattg tccggaggcc agcagcagcg cgttgctatt
      841 gcccgggcgc tggtgaatga accggtgatg attcttgccg atgaagcaac cggcaacctc
      901 gattccagaa catcttatga aatcatggcc ctgatccagg aactgaacca gcagcagggg
      961 aagaccatcg tatttgttac gcatgaaccg gatatagcga gtttcagcag ccggacgata
     1021 accttaaagg acgggaagat aataaaagac agcgccaatg agaaccggcg atccgcgaaa
     1081 gaagtgctgg ctgcgctgcc ggccgtggat gattatcaga acgaataaaa aacggaatat
     1141 aaaaatatga atgtcttaaa cctggtccgg atcgcctcca aagccctgca gcggaataag
     1201 ctccgcgcct ttctcacgat gctggggatc atcatcggcg tgggcgccgt tatcgccatg
     1261 gtggccattg gccagggttc caaaaaaagc atccaggacc agctatcgag catgggttcc
     1321 aacatgatca ccattcgccc gaacagcaat gtggtcgccg gggccaggct cgatgccggc
     1381 agtgtgcaga ccttaaagat tgaggacgta aaagcaatac aaaagcaggc gcagtatatc
     1441 aacgggattt ctcccgccgc ttcggccagg ggccaggcca ttaacggggc cctgaactgg
     1501 ccgacctcga tacagggcgt cagtccggag ttcctggata tccgcgcctg gaccgtcaaa
     1561 gacgggattg catttacgga tgcggatgtg actgctgcgg ccaaggtgtg cctgctcggc
     1621 cagaccgtgg tcagcaatct ttttgtaaac ggggagaatc ccattggtaa aataatccgc
     1681 tttaacaaaa tacccttccg ggtgatcggc gttctggggc agaaagggga aaatgccttc
     1741 gggcaggacc aggatgatat cattctcact ccctacacaa cggtacagga aagaatatta
     1801 tcgaccatct attttcaaaa tatttatgta tcggccaaga acgagcaggt caccgatgcg
     1861 gccaccgctg aaatctcaca gatcctgcgg gtcacgcacc ggttaaagcc gaccgatatc
     1921 gatgacttcg cggtacggac ccaggcggag ctgatcaaca cgttcagctc gacgagccag
     1981 ttgctgaccg tactgctcac ggccatcgcc ggcatttcgc tggttatcgg gggcattggc
     2041 atcatgaaca ttatgtatgt atccgtaacg gaacgcaccc gcgaaatcgg cctgcggatg
     2101 gccatcggcg cgagaggaat tgatatcctg ctgcagttcc tgatcgaagc catactgatc
     2161 agcatcaccg gcggtttgat cggcgtgggg ctgggtatta ttgcctcccg gctggtaact
     2221 caattcttat cgtggccgac cctgatctcc gaatcctcga tcgtactttc ctttcttgtt
     2281 tgtgctttta ccggcgtgtt tttcggctat tacccggccc agaaagcgtc ccgcctggat
     2341 ccgattgaag cattgagata cgagtaaggc gcctgaggcc tgaccgggtc agcctttccg
     2401 gtacgttata ccaaagaata ttatccgcaa accacggacg atcttactat tttatttacc
     2461 ttttaggtat cccggaattt aaaacttaaa actaaagacc atgaaaaatt tgctacttat
     2521 cggagtcacc acacttatcc tgtcgtgcaa taataacggc ggaaccgcaa ccaatccggc
     2581 ctccgattcg gttgccgccg gcgccgcctc caaagacagc gtactggaaa aaaataaggc
     2641 tacggctttg gccagcgaag aggctttcaa cagcggaaaa ctggacgatg catttaagga
     2701 tgccgtaccg gatgcggttg attacggcga tgggtccatg gcgccgatga aggggctcga
     2761 ttccataaaa gccggcgtaa aaatattcct gaacgctttt ccggattata agggagaaaa
     2821 tttcctcgcg atcggagagg gcaatcatgt ggccgtgttc ggggattggt caggcacatt
     2881 taaaaacccg ttgatgggta tgaaacctac gggtaaatcc ttcaagataa aagatgttga
     2941 cctgttcacc ttcaatgatc agggcaagat cacggaacat cgctccgtac aatccagcaa
     3001 aaccgttctg gatatggtgc aggctaaaat gatgaaaaag taatcccaaa tccatgttca
     3061 caataacgac cgccctggct aaaccggggc ggttgtgtga atggcgttga ttcggcttgt
     3121 cttgtgtttt ttctttaggc tgaggattgg gcctggagct tttaagggtc tgcgcgtcag
     3181 acaaactgcc tgaccagtat ttcccattcc ttttccaggc taagatcagg ttgttctgtt
     3241 ccggcctgcc ggtaccagta tccggcattt gaaatatctc cttctttccg gtgaaggtag
     3301 gcatgaaccc ttgcggcttc ccttccttcc aggtggtcca cgaggccgtg ggctttgtcc
     3361 cactcacctt tccggtcata ccacaaggct ttcaggaaaa cggaaaacgt atccggcggc
     3421 gactccgacc gcaggctatc ctgaacggat tgaagcgtat tattcatacc ttaaaaataa
     3481 tatttttacc cggggaaaat gtttttttag aaataccccg ccggaaacat taattctccg
     3541 ataaaagtaa atttatttca tggatatatt aaatcagcgc ctggttaacc agcagatagc
     3601 tgttcaccgt ttccatctgc cggaggagct ggtatgctgg caaggcgctg tccaggccca
     3661 ggactatgct atggcgaaat gggccatcgg atgccggctg gatggcatta ccgaaacgca
     3721 ggtggaaaaa gattttaatg aaggaaagat cctgaggacc catgtgctga ggccgacctg
     3781 gcattttgtt tcgccaaaag atatccgttg gatgttagcc cttacggccc cgaaaataaa
     3841 aacatttaca aaaggccatc accgggacct gggaatagat tccggaattt taaaacgcag
     3901 taaaaagata atagaaaaat tattgaaagg ggaagggccg aagacccgga aggaaatcga
     3961 acaggccctg aataaatccc ggatcggcac ggataataac cggctcggct tcctgctgat
     4021 cgatgccgag ctggacggcc tgctgtgcag tgccggcaga aagggaaagc aatttgccta
     4081 tggactgctg gaagagatcg tcccggaatt taaaacccgg aaaagagagg agggcatagt
     4141 ggaattggcc ggaaggtatt ttggcagtca tggtccggcc acggttcatg attttgcctg
     4201 gtggtcgggc ctttccctct ctgaaataaa aagaatttta cccggcatgg gatccggttt
     4261 gttgtcccaa aagatggatg gccaggacta ttggttttcc agatcgatgg cggaggcaaa
     4321 gggtggtcca aagggcattt attttttacc cgcatttgat gagtacttta tcggctaccg
     4381 cgacaggacg cacagcatgg acacctcttt cgcaaaacgg atcatgggcc cgaacgggat
     4441 ttttaatccg gtcatcgtac ggaagggacg gatcgccggc acctggaaaa gaaaagagga
     4501 gaagtcaggc tctatgattg aggcggctcc cttcgcttct ttcaaactat cagacagggg
     4561 agttgcatct gccatgaaaa aatacagcag atacctgggg agggcggtta gccttgctca
     4621 attttgattt atgatctttg ccgcattcgt ttcatataat ttctgcaata tatttttaga
     4681 caggtccagg ccgtagagcg gccagtgata atcgaacatc ccggtttcat aaaaatgttc
     4741 atccgcggtt tccaggatgc ggaaggtggt ccggtacata ttggccgcgg tacccatatc
     4801 ggttccgtat accagccggt cctggtacct ggtaataaag gcggaggcat agcgggggac
     4861 cggggcgatt tccgcaaacc gggcggcgat atcggcatac aggttcgggt atttgtcaaa
     4921 taaattcc
//
LOCUS       DASYCU010000415         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4147043, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000415 DASYCU010000000
VERSION     DASYCU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4147043"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGM24_08385"
     CDS             complement(<1..435)
                     /locus_tag="VGM24_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEY4155432.1"
                     /translation="MNRRSFLEKTGAVSAGTLLSSWKQTNPGPVIPLPAGTRLIILAT
                     NWGYGGSVDEFCEAAKKEGYDGIEMWWPTTSSAAEELFFALKKHSLEVGFLCAGSEKD
                     PAEHLNSFTKNLEAAVSNSYQRPVYINCHSGRDYFSMEDGFRF"
     gene            complement(446..1945)
                     /locus_tag="VGM24_08390"
     CDS             complement(446..1945)
                     /locus_tag="VGM24_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY4155433.1"
                     /translation="MMDPHKIMREAREESMRYQTRRHFLREASFGFGGLALAYLAGGC
                     KSSATDRMLQDLYDPVNPLAPRMPMFPGKAKSVIYLHMAGAPSQLELFDYKPELMKMD
                     GQDCPKSLLEGKRFAFIRGVPKMMGPQAQFKQRGESGAWISDHLPHLASIADELSFLK
                     AVTTDQFNHAPAQLLVHTGGPRLGRPSLGSWVTYGLGTENQDLPGFVVLTSGGKNPSA
                     GKSVWGSGFLPSVYQGVQCRSVGDPVLFLDDPDGMDRDLRKASIDAINEVNKQQYKTF
                     QDPETLSRISQYEMAYKMQISVPEVMNIRDEPEYIHEMYGTKPGEACFANNVLLARKL
                     VEKGVRFVQLFDWGWDDHGSDFDLSIDYGLINKCRQVDKPITALILDLKQRGLLDDVL
                     LVWGGEFGRTPMQENRDGKKMPFKGRDHHTDAFTIWMAGGGIQKGVSYGETDEIGYSP
                     VSGKTTAFDIQATILNRLGFDHEKFTYNFQGRPFRLTDVSGRVITEIIS"
     gene            complement(1952..>4014)
                     /locus_tag="VGM24_08395"
     CDS             complement(1952..>4014)
                     /locus_tag="VGM24_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEY4155434.1"
                     /translation="MWLDLARYADSKGYEKDENRDIWKYRDWLIHAFNSDKPYDQFLT
                     EQLAGDLLPNPTEQQLIATAFHRNTMTNDEGGTDNEEFRTAAVLDRVNTTWQALMGTT
                     FGCVQCHSHPYDPFRHEEYYKFMAFFNNTRDEDTFADYPTLRDYNDSAKAKMKELKDW
                     LQLNASAQRTKEIKEFLLTWEPSINSQNADLLVNSAIADTKWLAFRNHSSCRLTRVDL
                     DRKDQLIYRYAGYIPNGKCEVRLDRPDGPLAFSFNLPKKGDDWTVSSVDFPPLSGTHD
                     LYFSYSSPFLKKPEDTGAMMDWLFFTQQFPGKGKPGYESMKKRFWELDTMKTPTTPVM
                     IENPPDMRRKTYVFERGNWLVKGQEVQPDVPHSLNPFPKNAPRNRLGMAEWMVSRENP
                     LTARTIVNRLWEQVFGNGLAETLEDLGTQGIPPTHPELLDYLSWKLMNDDGWSLKKLL
                     KEILLSATYRQDSRVTKESEEKDPSNKYYSRGPRIRLTGEQIRDETLSVTGLLSEKMY
                     GPSVFPYQPKGIWLTPYGSSVWKKSEGEDQYRRAVYTYWKRTSPFPSMMSFDGPDREV
                     CTARRIRTNTPLQALVTLNDSAFLEAARHFADRLQKQNPGSADKQIRAGFEKAIGHDI
                     DENSFEALEKLYRKALLKYQQQPEKGKEMGGGNKTAASQPERSALIVVTNAILNLDEY
                     VTKN"
BASE COUNT          859 a         1084 c          986 g         1085 t
ORIGIN      
        1 aaaccggaat ccgtcttcca tggaaaaata gtcgcgcccg gaatgacaat taatatagac
       61 gggccgctgg tatgaattgg ataccgctgc ttccagattt ttagtgaagc tgttcaggtg
      121 ttcggccgga tctttctcgg aacccgcgca aagaaaaccg acttccaggg aatgtttttt
      181 cagggcgaaa aataattctt ccgctgcgga ggaagtggtg ggccaccaca tctcgatccc
      241 gtcatatcct tctttttttg cggcttcgca aaattcatcc acgcttccac catagcccca
      301 gttagtagcc agaataatga gcctggtccc ggccggcagg gggataacag gtcccggatt
      361 ggtttgcttc caggatgaca ataaggttcc ggcgctcacc gcaccggttt tttccaaaaa
      421 agatcgcctg ttcaatgtgt ttattttagg aaattatttc agtgatcaca cgcccgctca
      481 catcggtcag ccggaaaggc ctgccctgaa aattatacgt gaacttttca tgatcaaatc
      541 ccagccggtt cagaatggta gcctggatat cgaatgcggt tgttttacca ctgaccgggc
      601 tgtaaccgat ctcatccgtt tccccatagc taaccccttt ctgaatgcct ccgccggcca
      661 tccagatggt aaatgcatcc gtatgatgat cccttccctt gaagggcatt ttcttcccgt
      721 cccggttttc ctgcatgggc gttcttccaa actcccctcc ccacaccagc agcacatcat
      781 ccagtaatcc tctttgtttg agatcgagga tcagcgccgt gatgggctta tccacctgcc
      841 ggcacttatt aatcagcccg tagtcgatgg aaagatcaaa atcgcttcca tggtcgtccc
      901 atccccagtc gaacaactga acaaaccgca cccctttctc caccagtttc cgggcaagca
      961 gcacattgtt ggcaaaacag gcttcccccg gtttggtgcc gtacatttcg tgaatatatt
     1021 ccggttcatc ccggatattc atcacttccg gaacagagat ctgcatctta taggccatct
     1081 cgtattggga aatgcgcgag agggtttccg ggtcctgaaa agttttgtat tgttgtttgt
     1141 tcacttcatt gatggcatcg atggatgctt tgcgcaggtc cctgtccatg ccgtccggat
     1201 catcgagaaa caataccgga tctcccacgc ttctgcattg cactccctga tatacgctgg
     1261 gcaggaagcc gcttccccag acgctttttc cggcagacgg attttttcct cccgaggtta
     1321 ataccacaaa cccggggaga tcctgatttt ccgtacccag tccgtaagtg acccaggatc
     1381 ctaaactggg tcttcccagc cgcggacctc cggtatgtac cagcaattgg gccggcgcat
     1441 gattgaactg atcggtggtt acggctttca aaaaactaag ttcatccgca atgctggcca
     1501 ggtgcggtaa atgatcgctg atccaggcgc cgctctcccc tctttgtttg aattgcgcct
     1561 gggggcccat cattttagga acaccccgga taaaagcaaa tcgttttccc tccagcaggg
     1621 atttcgggca atcctgcccg tccatcttca tcagctcggg tttgtaatca aaaagctcca
     1681 gctgggaggg cgccccggcc atatgcaggt agatgaccga tttggcctta ccgggaaaca
     1741 tcggcatgcg gggtgccagt ggattcaccg gatcataaag atcctgcagc atccggtctg
     1801 ttgcggaact tttacatcct cctgccaggt aagcaagcgc cagtccgccg aatccaaagg
     1861 acgcttcacg taaaaaatgc cggcgggttt gataacgcat cgattcctcc cgcgcttccc
     1921 tcattatttt atgcgggtcc atcatgtttt actaattttt tgtgacgtat tcatccagat
     1981 ttaaaatcgc attggtcacc acgatcaggg cagaccgttc cggctggctg gcagcggttt
     2041 tgtttccgcc tcccatctct tttccttttt cgggttgctg ctgatatttc aataaggctt
     2101 tccggtaaag tttttccagc gcctcgaaac tgttttcatc aatatcatgc ccgatagcct
     2161 tttcaaatcc ggcacggatc tgtttatcgg cagatcccgg gttttgcttc tgcaaccggt
     2221 cggcaaaatg tctcgctgct tccaggaaag ccgagtcgtt caaggtgacc agggcctgca
     2281 aaggcgtatt ggtgcggatc ctgcgggctg tgcagacttc ccggtccggc ccgtcaaagc
     2341 tcatcatgct ggggaaaggg cttgtccgtt tccagtacgt atatacggcc cgtcggtatt
     2401 ggtcctctcc ttcacttttt ttccatacgg aagacccgta aggcgtgagc cagatgccct
     2461 tgggctgata cggaaacaca ctgggcccgt acatcttttc actcagcaat cccgtaaccg
     2521 aaagcgtttc atcccggatc tgttcaccgg tgagcctgat gcggggtccg cggctgtaat
     2581 atttattgga tggatctttt tcttcgcttt ctttcgtaac ccgggaatcc tgacggtaag
     2641 tggcggagag caggatctct ttcagcagtt tttttaaact ccatccgtcg tcgttcatta
     2701 atttccagct caggtaatcg agcagctcgg gatgggtggg tggaattccc tgcgtaccca
     2761 ggtcctccag ggtttcagcc aggccatttc cgaaaacctg ttcccacaaa cggtttacga
     2821 tagttcttgc cgtgagcggg ttttccctgg aaaccatcca ttcggccatt cccaaacgat
     2881 tcctgggcgc atttttcgga aacgggttaa gagaatgagg aacatcgggc tggacctcct
     2941 gccctttcac cagccagttt cctctttcaa atacataggt cttccgacgc atgtccggcg
     3001 gattttcgat cataaccggt gtggtggggg ttttcatggt atccagttcc cagaatcttt
     3061 ttttcatgga ttcatagccg ggttttcctt tgccgggaaa ctgctgcgtg aaaaataacc
     3121 agtccatcat ggctccggta tcttccggtt tttttaagaa ggggctggaa tacgaaaaat
     3181 aaagatcatg cgtcccgctg agcggtggaa aatccacgct gcttacggtc cagtcatcgc
     3241 cttttttcgg aagattaaaa ctgaaagcca gcgggccatc cggacggtcc agccgcacct
     3301 cgcattttcc gttgggaata tagccggcgt accggtagat gagctgatct ttccggtcga
     3361 ggtccacccg ggtgaggcga cagctggaat gattccggaa tgccagccat ttggtatcgg
     3421 caatggcgct gttgaccagg aggtcggcat tctgggaatt gatcgaaggt tcccaggtga
     3481 gtaaaaattc tttgatctct tttgtccttt gcgcactggc gttcaactga agccagtcct
     3541 tcagctcttt catttttgct ttggcggaat cgttataatc cctgagcgtg gggtagtcgg
     3601 caaaagtatc ttcgtcccgc gtattattga agaaggccat gaatttgtaa tattcctcgt
     3661 gccggaacgg atcgtaagga tgactatgac attgaacgca tccgaaagtg gttcccatca
     3721 gggcctgcca ggtggtgttc acccggtcaa gcacggctgc cgttctgaat tcctcattat
     3781 ccgttccccc ctcatcgttg gtcatggtat tccggtggaa tgccgtcgcg atcagctgtt
     3841 gctcggtggg gttgggaaga aggtccccgg ccagctgctc cgtgagaaac tgatcatagg
     3901 gcttatccga attgaatgca tggatcagcc agtcccggta tttccagata tcgcgatttt
     3961 catctttttc atatccctta ctgtcggcat accgggccag gtcaagccac atcg
//
LOCUS       DASYCU010000416         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_235371, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000416 DASYCU010000000
VERSION     DASYCU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235371"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VGM24_08400"
     CDS             <1..963
                     /locus_tag="VGM24_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4155435.1"
                     /translation="SVEKGSETGIRSEIAAVSKRDRSADIILPEMASDNSPSLSMKEK
                     GSAQKNLPDLAEISDAVTASEKTITNRNPMRNDRFRWEIYISPTLNTRHLDGVDYHNV
                     SQTIQNAPITVVRFANVNGFVDNTPAMGYDLGGNVLYRLSQSLSLKAGLQFSFSRYYI
                     NAYNSNQPQSSAALNSYFGYIADSISNNSQGRNVAQKNPQHYQNKYYQLSMPVGIEWK
                     VAGKGRLQFHAGATIQPGYLLNRDAYVLSSDYSSYSKEPAALRKWNLNAGAEIFLSYR
                     LGKIRWELGPMLRYQILSTYKDSYPLKENLMNYGIRLGFSKTIR"
     gene            987..1589
                     /locus_tag="VGM24_08405"
     CDS             987..1589
                     /locus_tag="VGM24_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155436.1"
                     /translation="MKLSCILIIVLPFLFFLACQPVSSPLAPAGPGMTDTVKKNYFPV
                     QNFLQGEIAYVDSIPFKLMEYQTRNGKTDSGIITTQKFDGLARAFLPEGLDSSRFEKR
                     FNESSFLDQTNNLLTFTYSTKDTSFGLRRVDVLATQDPGAADKVKSIYLEVSSGSRDS
                     LVLRKMYWRSKKSFSILQIYQPAHGEAMTSQTTVVWDSSK"
     gene            1594..3423
                     /gene="uvrC"
                     /locus_tag="VGM24_08410"
     CDS             1594..3423
                     /gene="uvrC"
                     /locus_tag="VGM24_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY4155437.1"
                     /translation="MDQQAFQEIYHTIPPDAGIYKYFDPKKELLYVGKAKNLRKRVSS
                     YFNKTFTNYKTYELVRRISSIEFTIVNSEQDAFLLENSLIKQFQPRFNINLKDDKSYP
                     YIIIKKEPFPRVFLTRRKVDDGSEYLGPFTSVAKVRELIDFIKSNVPLRTCKLNLTPA
                     NIAKKKFRVCLEYHLGNCKGPCEGLQSEADYLEGLKKIKNMLKGNLGPVISGFRLEMK
                     EAAGNMQFEKAEAIRKKIEHLENYQARSVIVNRHIEQADVFSIFRDNDGAFINYLMVQ
                     NGTIVQTHTTEAKPQLEETDEEILAFSIAQLRSTFNSQAPEIIVPIPLEYPEEGVHIT
                     VPKGGDKKKLLELSEKNVNYYREEAEKKKMLHLVKKTPEERIGVLEQLQKDLQLSSLP
                     VHIECFDNSNFHGSYPVSAMVCFRDGVESKKDYRHFNVKTVTGINDFATMKEVVYRRY
                     KRMSEENASLPQLVIIDGGKGQLHSALESIEELGLRGKMTLVGLAKNEEEIFFAGDRE
                     SLKLPYDSISLKFIRKIRDEVHRFGISFHRNKRSRNTFQNELENIMGIGKQTADQLLK
                     TFHSVKKVKGATEEELRAVVGDAKAKIITTFFLRNREEETQAG"
     gene            complement(3702..4742)
                     /gene="gldN"
                     /locus_tag="VGM24_08415"
     CDS             complement(3702..4742)
                     /gene="gldN"
                     /locus_tag="VGM24_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HEY4155438.1"
                     /translation="MKKRLIRLSLLAAMFALAVGTVDAQAPAHRKKTKRSTTKKKTNT
                     SRNSAANTAVVPPKDTVAPKPVTEFVADIKLDTPRKSLRNDASIELNLVKDRTPLAYE
                     HIREDDAVYRQRVWREIDVHEKMNLPFVYKATEDNGNQRFINILLNLIKNGDVTAFDA
                     TIDDRFTTPMTFRQIAESMVGKPTVIQIPDMTVDPDGSKGITKDTTIVQDFNPDQVER
                     YEIKEEWVFDKESSRMYARILGIAPEKTILNPDGSFRAVVPIFWVYYPDLRQYLAKYE
                     AYNGKNYGARMSWEELFESRMFSSRIIKSTIDNPGDNFINAYIKDPILDLLEGDNIKD
                     KIFNYEQDLWSY"
BASE COUNT         1343 a         1144 c         1173 g         1209 t
ORIGIN      
        1 tccgttgaaa aaggatctga aaccgggata agatctgaaa tagctgctgt ttcaaaaagg
       61 gatcgctctg ccgatatcat tcttcccgaa atggcttcag acaacagtcc ttccctgagc
      121 atgaaggaaa aagggtccgc acaaaaaaat ctgccggatc tggcggagat atccgatgcg
      181 gtgaccgctt cagaaaaaac aattacgaac cggaacccta tgcggaacga ccgcttcaga
      241 tgggaaatct atatttcccc gaccctgaat acgcgacacc tggacggcgt ggattatcat
      301 aatgtaagtc agactattca aaatgccccg ataaccgtgg tgcgctttgc caatgtgaac
      361 ggctttgtgg ataacacccc ggccatggga tacgacctgg gcgggaatgt gctttaccgc
      421 ctttcccagt ccctttccct gaaagccggg ctgcagttca gtttcagccg gtattatatc
      481 aacgcctata actcaaacca gccacagtct tctgctgcat taaattctta tttcggatat
      541 attgctgatt cgatttccaa taattcgcag ggaagaaatg tagcgcagaa gaatccgcaa
      601 cattaccaga ataaatatta tcagttatcc atgcccgtag gtatcgaatg gaaagtggcg
      661 ggcaaaggca gattgcagtt tcatgccggc gcaaccattc agcccggtta cctgctgaac
      721 cgggacgctt acgtgctttc cagtgattac agcagctact ccaaagaacc cgctgcgctc
      781 agaaaatgga atttaaatgc aggagcagag atctttcttt cataccgcct gggcaagatc
      841 cgttgggaac tcggcccaat gctccgctac cagatcctct ctacttacaa agacagttat
      901 cctttaaagg agaacctgat gaattacggc atccggctgg ggttcagcaa aacgatcagg
      961 tgattttgtt ttcttaattt tatcggatga agttatcctg catcctgatc attgttctgc
     1021 cgtttctttt tttccttgcc tgccaaccgg tttcttcccc gcttgctccg gccggccccg
     1081 gtatgacgga taccgttaaa aaaaattatt tccccgtaca aaattttctc cagggcgaaa
     1141 tcgcgtacgt agattccatc ccctttaaat tgatggagta tcaaacccgg aacggaaaaa
     1201 cggattccgg cattattacg acccaaaaat tcgacgggct ggccagggcg tttctgccgg
     1261 agggcctgga cagcagccgg tttgaaaaaa gatttaacga atcctctttc ctggatcaga
     1321 ccaacaacct gctcaccttt acctactcaa caaaggatac cagtttcggg ttaagaaggg
     1381 tggacgtgct ggcaacgcag gatcccgggg ccgcagacaa ggttaaatcg atttacctgg
     1441 aagtgagttc cggcagcagg gacagcctgg tgctcagaaa aatgtactgg aggtccaaaa
     1501 agagcttcag tatccttcaa atttatcaac ccgcacacgg cgaagccatg acgagccaga
     1561 caaccgtggt gtgggacagc agcaaataac cggatggacc agcaagcatt tcaggagatt
     1621 tatcatacca ttccgccgga tgccggaatc tataagtatt tcgatccgaa aaaggaattg
     1681 ctgtatgtgg gaaaggcgaa gaatttgcgt aaacgcgtaa gttcctattt taataaaacc
     1741 tttaccaact ataaaaccta tgagctggtc aggcgcatca gctcgatcga atttaccata
     1801 gtgaattctg agcaggatgc ttttctgctg gagaattccc tgatcaaaca atttcagccg
     1861 cgcttcaata tcaatcttaa ggatgataaa tcctatccct atatcatcat caaaaaagaa
     1921 ccctttcccc gcgttttcct tacccgccgg aaggtggatg acgggtcgga atacctgggt
     1981 ccctttacgt ccgtggcgaa ggttcgtgag ctgatcgatt ttattaaatc aaatgttccc
     2041 cttcgtacct gtaagctgaa tctgacgccg gcgaatatcg cgaaaaaaaa attcagggtt
     2101 tgcctcgagt atcatttggg caattgcaaa ggcccctgtg aaggattgca atcggaagca
     2161 gattacctgg aaggattgaa gaaaataaaa aatatgctca agggaaatct cggtccggtc
     2221 atcagcggtt tccggttgga aatgaaagaa gcggccggga acatgcaatt tgaaaaagcg
     2281 gaagcgatcc gtaaaaaaat cgaacacctt gaaaactacc aggccaggtc cgtaattgta
     2341 aaccggcata tcgaacaggc cgatgtgttt tcgatattcc gggacaatga cggggcattc
     2401 atcaactacc tgatggttca aaacggaacc atcgtccaga cacataccac ggaagccaaa
     2461 ccccagctgg aagaaaccga tgaggagatc ctggcttttt ccattgcgca attgcgcagc
     2521 acgttcaaca gccaggcgcc ggagatcatt gtgccgattc cgctcgaata cccggaagaa
     2581 ggcgttcata ttaccgttcc gaagggcgga gataaaaaga aattgctcga gctttcggaa
     2641 aaaaatgtga actattaccg cgaggaagca gaaaagaaaa agatgcttca cctggttaag
     2701 aagacgccgg aagaaaggat cggggttttg gagcagcttc agaaagacct tcagctttcg
     2761 tctttaccgg tccacattga atgctttgat aattccaatt ttcacggaag ctatccggta
     2821 tctgcgatgg tttgttttcg cgatggcgtg gaaagcaaaa aggactaccg ccatttcaat
     2881 gtgaaaaccg taaccggaat caatgatttc gctacgatga aagaagtagt ctaccggcgg
     2941 tacaaacgaa tgagcgagga aaacgcctcc ctcccccagc tggtgatcat agacggggga
     3001 aaaggccagc tgcactcggc gctggagagc attgaggagc tcggtttacg cggaaagatg
     3061 accctggtcg gactggccaa gaatgaggag gagatctttt ttgccggcga ccgggaatcc
     3121 ctgaagctcc cttacgacag catcagcctg aagtttatcc gtaagatccg ggatgaagtt
     3181 caccggtttg ggatcagctt ccaccggaat aaacggagcc ggaacacctt tcaaaatgaa
     3241 ctggaaaata ttatggggat cgggaaacaa acagccgatc agctgcttaa aacatttcat
     3301 tccgtgaaaa aagtaaaagg ggcgacggag gaggaactcc gtgcggttgt cggagatgcc
     3361 aaagcaaaaa tcatcacgac tttcttttta aggaacaggg aggaggaaac gcaggctgga
     3421 tagaggtttc agcccggagc cggatataaa taaaaagggg ccagaatgga aattcagccc
     3481 cgtttttaaa atccaatatc ctatgaaaaa ccgttgtaaa aatagcaaaa caattctatt
     3541 aaacaagaac ccttaggtgt attttgaaaa taattatcaa cactttaacc gggtcatttt
     3601 tctccggatt tgcgtataaa tactaagctc ccaaattggg aatggggcca aacaaaaagg
     3661 ccttctgcgt gcagaaggcc ttttaaataa ggtttacccg tttaataaga ccagaggtcc
     3721 tgttcgtagt tgaagatctt gtccttaatg ttatctcctt ccagcagatc caggatcgga
     3781 tccttgatat aggcattgat gaaattgtcg cccgggttat caatagttga tttgattatc
     3841 cggctggaga acatacggct ttcaaaaagc tcttcccagc tcattctggc tccgtaattc
     3901 tttccattat atgcttcata cttagccagg tactgcctca ggtcgggata gtatacccag
     3961 aagatcggga caactgcccg gaaagagccg tctgggttca ggatcgtttt ttcgggtgcg
     4021 atgccgagga tacgggcata catcctggag gattccttgt caaaaaccca ttcttccttg
     4081 atttcatagc gttcgacctg gtcggggttg aagtcctgga cgatggtcgt gtctttggtg
     4141 attcccttag aaccatccgg atcgacggtc atgtccggaa tctggatcac ggttggctta
     4201 cccaccatgc tttcggcaat ctgccggaaa gtcattgggg tggtgaaccg gtcgtctatc
     4261 gtcgcgtcaa aggcggttac atcgccattc ttgatgaggt tcagcaggat attaatgaaa
     4321 cgctggttac cattgtcctc tgtagcttta taaacaaacg gcaggttcat tttttcatga
     4381 acatctattt cccgccaaac gcgttgacgg taaaccgcat catcttcccg gatatgctcg
     4441 taggccagtg gcgtacggtc ttttaccagg ttgagctcaa tggatgcgtc attccgaagc
     4501 gatttgcggg gagtatccag tttgatatct gctacaaatt cggtaaccgg cttcggcgcc
     4561 accgtatcct tgggaggaac caccgctgta ttggcagcgc tgttccggct ggtattggtt
     4621 ttcttctttg tagtagacct tttggttttc ttcctgtgtg caggcgcctg cgcatccacg
     4681 gttcccacgg ccagcgcaaa cattgctgcc aggagggaca atctgatcaa acgcttcttc
     4741 atgttctaaa atttatcgtg agaaaaggac aaacaggatc cgccaggttt agcctggcgg
     4801 atccgaaata ttttatttaa gactaaatac catgggcgca atctcgcgtt ctttgccatc
     4861 cggtccttt
//
LOCUS       DASYCU010000417         7177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4003471, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000417 DASYCU010000000
VERSION     DASYCU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003471"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VGM24_08420"
     CDS             complement(<1..921)
                     /locus_tag="VGM24_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY4155439.1"
                     /translation="MPGNILLIDDEEPLRKLLSRIIGLEGFKVDQAGTLKSGFNTLRE
                     KEIDVVLCDVRLPDGNGVDFIKQAKAEYPLLEIILLTAYGNIPDGVQAIKNGGFDYIS
                     KGNDNDRIVPLLHQAVDKVIAQRKAAVKIRNKGGYDFDDIIGHSPLIKEAILQARKIA
                     RTDANVLLLGETGTGKEVFANAIHAHSKRSAKAFIAINCSAFTRELLEGELFGHKPGA
                     FTGATQEKKGLIELANEGTLLLDEIGELNMDVQSKLLRVIENREFIKLGDTKTTHVDV
                     RIIAATNRELAKMAETGEFREDLFYRLNVFA"
     gene            complement(995..1558)
                     /locus_tag="VGM24_08425"
     CDS             complement(995..1558)
                     /locus_tag="VGM24_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155440.1"
                     /translation="MPNPHALIQDFFSALSLPRYRKYIFSTLKAAGSAHYWKKEDPAS
                     LLYFQKKMEDLLDAAHLLLKKPLGKKTERKKSVLRPESLAAAEIDQALYLDKSSGLSQ
                     WEAFPRSLSEKEFLNPRIAFEAFFRHRSLKEWKADFGEIIFYALTPHSCSEDLVDLDL
                     LKTNRLLQKLIEAAHLIWVREGGSFRD"
     gene            1874..2308
                     /locus_tag="VGM24_08430"
     CDS             1874..2308
                     /locus_tag="VGM24_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4155441.1"
                     /translation="MSVFSRNLRFLRKQRGLNQDEISVLFKKGGNTVGNWENSKSEPN
                     IGELVKLADYFKVGLHDLLHTDLQKATLNALDLVSSPGLEKPSAGDYAVNDSSTSLAQ
                     DSNSDSFWLILRELRSINEKLDGLKGVYESGQHKPGSDKSSH"
     gene            2868..3758
                     /locus_tag="VGM24_08435"
     CDS             2868..3758
                     /locus_tag="VGM24_08435"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEY4155442.1"
                     /translation="MKTLSKLVSTSSVSLLVITSPVLISSNHKKVDLFTSTSAVAATY
                     HIPVLTDNTIKHISYTSPAVTTVIAEKVSSASVAKLDYSVSAAAKKAFEVKMVIQQAM
                     TLYDSMKLGESGLDQKAFEYAWRGYHNLMKTGGIKKAGVLSICDFTQSSRNKRLYVID
                     VANKKLLYNTYVSHGMNSGVEYATSFSNRPESYKSSLGFFLTTKTYFGRNGLSLKVKG
                     LEKGYNDLAARRHIVLHGSDYITQDYLKDNGEMGRSLGCAAMPNAVTPKIIRTIKNGS
                     CLFIYSPSVKYLNASSVING"
     gene            3862..6180
                     /locus_tag="VGM24_08440"
     CDS             3862..6180
                     /locus_tag="VGM24_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155443.1"
                     /translation="MNGRILAFCVSCLLMNAAESQSLDSSISLYSSRSEQEKIHIHFD
                     KDAYLPGETVWMKAYLLSESKPSYISKNIYFDWTDARGHLLRHSVSPITEGTAISSFV
                     IPSSAENGVVHVKAYTQWMLNFDDAFLYNRDIPVLVPSNEFNAAPEKIKPEINFFAEG
                     GDLVSGVNSLLAFEALDQHGRPVTVNGVIKNSNNAVLDSFHTAYLGMGSFFLKPAAGE
                     TYQAVWKDETGASHTTALPQTRPNGVVLHLVSNKNDQIQFSLERSVDAVSLNRLTVIG
                     TIEQQVVYKNKLNLVNFRGDGSIATASFPCGIMQLTVFDADMTPIAERVIFVNNRKPY
                     GNVQMNRELVSLDKRARNEISLEIPDSLSTNLSISVTDGGLGADSSHNIYSDLLLSAD
                     LKGKINDAASFLQNSENENDRMNLLLLTHGWRRFNWEAVVSGKLPALKYLHDADFLTL
                     KGEIRGSAGSLDEADSMALLLISKDRKKHVMNLPVNGDGKFNQRGLYFYDSLQVVYRF
                     NHLSKINKGTEISLFSNLLPPLTPARAEEPSYSWMKVPDVVLEKEFNGSIMETSDYSV
                     PSDDMSYIVTPKQEAQKGASETAAHYLSTMFADLHFPATLKDHATGDARLTSYSTGGA
                     ASVRANVNISIDGVPVAMDNLKSVSMKEVLFIKFVPKTSPKALPTLAISSRQAEQQSL
                     IMGNKTGFAVVTGYTAVREFYAPHYTDKISDYAASDFRSTLYWDPEVKLDRSHRKVKL
                     VFYNNDITTKFRIVIEGMNQEGKLTRIEELIK"
     gene            6212..6481
                     /locus_tag="VGM24_08445"
     CDS             6212..6481
                     /locus_tag="VGM24_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155444.1"
                     /translation="MLSVEFTFSVLALIGIIAGSLLTGFLLRSRQLQKKQFKISELRK
                     EIVYNHAQILELQKEYVMLDKTIKGNMAPVLPMKQVVAEKKEMAL"
     gene            complement(6533..>7177)
                     /locus_tag="VGM24_08450"
     CDS             complement(6533..>7177)
                     /locus_tag="VGM24_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY4155445.1"
                     /translation="DAIRSVLLTANGRAFSSGQDLSEFPGGKLPVFEEVIEEYYNPVI
                     RLLRTIRKPVLCAVNGIAAGAGANIALSCDIVVATASASFVQAFSKIGLIPDCGGTYF
                     LPRLVGLQKATAFMMLGDKITAAEAEQNGMIYKCFEDDRFEESAAQIAGTLAMLPTTA
                     LMLTRNALQQSFTNTLDQQLETEKNLQGQAGHTRDFKEGVTAFFEKRMPSFTGK"
BASE COUNT         1924 a         1738 c         1665 g         1850 t
ORIGIN      
        1 ggcaaaaaca ttcaggcgat aaaataaatc ttctctgaat tccccggttt ccgccatttt
       61 agccagctcc cggttggttg ccgctatgat ccggacatcc acatgggttg ttttggtatc
      121 ccctaatttg atgaactccc ggttttctat tacccggagt aatttggact ggacatccat
      181 atttaattcc ccgatttcgt caagaagcag ggtgccttca ttggcaagtt ctattaaacc
      241 ctttttttcc tgcgttgcgc cggtaaaggc gcccggttta tgcccaaaca attccccttc
      301 caaaagttcc ctggtaaaag cgctgcaatt aatggcaatg aatgcttttg cggacctttt
      361 gctatgggca tgaatggcat tggcaaagac ttccttgccg gttccggttt ctcccagcag
      421 cagaacattt gcgtctgtcc tggcgatttt cctggcctgg aggatggcct ctttgattaa
      481 cgggctatga ccgatgatat catcgaaatc ataaccgcct ttattcctga tcttaaccgc
      541 ggctttcctt tgggcaatga ccttatccac cgcctggtga aggagcggaa cgatgcggtc
      601 attatcgttg cccttgctga tatagtcgaa acctccattc tttatcgcct gcaccccgtc
      661 aggaatattt ccgtaagcag tcaggagaat tatttccaaa agcggatatt ccgcctttgc
      721 ctgctttata aaatccactc cgttcccatc cggcagcctt acatcgcaaa gaacgacatc
      781 tatttctttc tcccgcaatg tgttaaaccc ggatttcagg gttccggcct ggtccacttt
      841 gaaaccttcc agcccaatga tccggctcaa cagcttacga aggggttctt cgtcgtctat
      901 cagtaaaata tttcccggca tattggaaac aaagctatgg aataaagatc tcgcggacag
      961 ttgccggcgc ctcctcctga tattcctaaa gagttcagtc cctgaacgat ccgccttccc
     1021 taacccagat caggtgcgcc gcttcgatta gtttttgcag gagccggttt gttttcaaca
     1081 aatccagatc aaccaggtct tccgaacaac tgtgcggtgt aagggcatag aatatgatct
     1141 ctccgaaatc ggctttccat tccttcaggc tccgatggcg gaaaaaagcc tcaaaagcga
     1201 tccggggatt gagaaactct ttttccgaca gactcctggg aaaagcttcc cactggctga
     1261 ggccggatga tttgtcaaga taaagcgcct ggtcaatctc tgccgcagcc agtgactccg
     1321 gtcgaagaac agacttcttc ctttccgttt ttttacccaa gggcttcttt aacaggagat
     1381 gggccgcgtc caggagatct tccatctttt tctgaaagta gagcaggctg gccggatctt
     1441 cctttttcca gtaatgcgcg ctgccggcgg ctttaagggt agagaaaata tattttctgt
     1501 aacggggaag ggataaggca gaaaaaaaat cctgtataag ggcgtgtgga tttggcataa
     1561 gagatcttta gtgagtaaaa aaggctggga aatggcccat tttcagccga aaaaggagaa
     1621 tttctgcctt ttttggccca aatgacccat tcatcagatt aagactaccc agtttgtagt
     1681 attgctcaga atctctaaaa tcggaacttc agcgttaaaa atttggatgg aacccgttta
     1741 tctgccctaa ccagggagca gaaaattggt gaaatagatg cagaattgta tatttgcgta
     1801 aatatttagc taagttaata aaatcttgga gcgtttccaa atttgtttga taaattcttt
     1861 agcaaggtca tccatgagcg tattttccag aaatcttcgc tttttaagga agcagcgggg
     1921 cctgaaccag gacgagattt ccgtcctttt taaaaagggc gggaataccg tgggtaactg
     1981 ggagaattcg aaaagcgaac cgaatatcgg ggaactggtg aagctggctg attattttaa
     2041 ggtcgggtta cacgacctgt tgcataccga tctgcagaaa gccactttga acgccctgga
     2101 cctggtctcg tcgccgggtt tggaaaagcc ttccgcgggg gactatgccg taaacgattc
     2161 ctccacgagc ctggcccagg acagcaacag cgattccttc tggctcatcc tgcgggaact
     2221 gagatccatc aatgagaagc tggacggact aaaaggagtc tatgagtccg gacagcataa
     2281 gcccggatcc gataaaagca gtcattaatt attatcaaac cggccataat tttattaaac
     2341 cggcctgcat ccggagcctc tttctcccga atccggaaaa attttccaaa cggtggaagg
     2401 atccggcctg ccggcccttt ttccggcacg catttcggct tcttaaaata ctttctaatg
     2461 gcttcagatt gaacagggta caacggcctg gggcttccgg tagctttcct ttttatactt
     2521 tggtatcaag cttgaaatag tagatatagt tctttacatc cggaagcaat tgaagctgtc
     2581 cattcgtcga aaaaattatg agttactctg attaattgca atctttgatt ccgataacgt
     2641 acccatccca cctgtaaacc gaacgcaata acaggctgat gaaatcgtta ttgctgctaa
     2701 gagtaatcaa taataaaagt gcgctgaaac aagatcctta ggacaatcca tccttaagaa
     2761 aaatctcctg tttactacgt atctgagtag ccgacgaaag acccgtccgg taatgaacta
     2821 ccggctgatt tgactcgaac cttttgaaaa cttttaaacc cttcgtcatg aaaacgttgt
     2881 ctaaactcgt atccaccagt agtgtgtccc tcctggtcat tacatcaccg gtactgattt
     2941 caagtaatca caaaaaagta gatttattta ccagcacttc cgcagtcgct gccacgtatc
     3001 atattccggt acttaccgac aataccatta agcatatttc ttatacctct ccggccgtaa
     3061 ccacggtgat agcggagaag gtttcctctg cttccgttgc gaaactggat tattccgttt
     3121 ctgctgccgc aaaaaaagcc tttgaagtga agatggttat ccagcaggct atgacacttt
     3181 atgattccat gaaactgggt gaatccggac tggatcagaa ggctttcgaa tatgcctgga
     3241 gaggatacca taacctgatg aagaccgggg gcatcaagaa ggcaggcgtt ttatccatat
     3301 gcgattttac ccagtcttcc cgcaacaaga ggttatatgt aattgatgtt gccaacaaaa
     3361 aattattgta caatacgtat gtgtctcatg gtatgaattc aggtgttgaa tatgccactt
     3421 ctttttcaaa cagaccggaa tcttacaaaa gcagtttagg ttttttcctt accactaaaa
     3481 cttattttgg acggaatggc ctttccctga aggtaaaggg tcttgaaaaa ggatataatg
     3541 acctggctgc ccgcagacat atcgtattac atggttcaga ttatattact caggactatc
     3601 tgaaagacaa cggtgaaatg ggaagaagtc ttggttgtgc ggctatgccc aatgccgtaa
     3661 ctccgaagat catccggacg atcaagaacg gaagttgttt gttcatctac agccctagtg
     3721 taaaatacct gaacgccagc agcgtaatca atggataaac aaaaataaac gaaatacaaa
     3781 ggggtcagat tactgtcccc ttttttgacg attaggatct gttgattgag ctgaataaag
     3841 tagaatccaa accctttgaa tatgaacggc cgaatccttg ccttctgtgt tagctgcctg
     3901 ctgatgaatg cagccgaatc ccaatcgctg gattcttcga tcagtttata cagcagccgg
     3961 tccgagcagg aaaaaataca catccacttc gacaaagatg cttatctgcc cggagaaacc
     4021 gtttggatga aggcctacct gctttccgaa tcaaaacctt cctacataag caagaatatt
     4081 tatttcgact ggaccgatgc ccggggccac ctgctccgcc acagcgtttc tcccataacg
     4141 gaaggtacgg ccatcagctc ttttgtaatt cccagcagcg cagaaaacgg cgtggtccac
     4201 gtgaaggcct atacgcaatg gatgctcaat ttcgatgatg cctttttata taaccgggat
     4261 attccggtcc tggttccttc caatgagttc aatgcggcgc cggaaaagat aaaaccggag
     4321 atcaatttct tcgcagaagg cggagacctg gtaagcgggg taaattccct cctggctttt
     4381 gaagccctgg accagcatgg cagaccggtt accgttaacg gcgtaataaa aaacagcaat
     4441 aacgcggtcc tggattcatt ccataccgca tatcttggaa tgggttcatt ctttcttaaa
     4501 cccgctgccg gcgaaaccta ccaggccgtc tggaaagatg aaaccggggc atcgcatacc
     4561 acggcgctgc cgcaaacccg gccaaacggt gtcgtattgc acctcgtttc aaataaaaat
     4621 gaccagatac aattcagcct ggaaagatcg gttgatgcgg ttagcctgaa ccggcttacc
     4681 gttatcggaa cgatcgaaca gcaggtggtc tataagaata aactgaacct ggtaaatttc
     4741 cggggagatg gctccatagc caccgcttcc tttccctgcg gcatcatgca gttaacggtt
     4801 ttcgatgcgg atatgacgcc catcgccgaa cgcgtgatct tcgtcaacaa ccggaaacct
     4861 tacggaaatg tccagatgaa ccgggagctg gtcagcctgg ataaaagagc acggaatgaa
     4921 atttcgctgg agatcccgga tagcctgtcc accaaccttt ccatttcggt tacggatggg
     4981 ggactgggag cagacagcag ccataatatt tattctgacc tcctgctgag tgccgacctc
     5041 aaagggaaga tcaacgatgc ggcttctttc ctgcagaatt cagaaaatga aaatgaccgg
     5101 atgaacctgc tgctgttaac ccatggctgg agaagattca actgggaagc ggttgtttcc
     5161 ggaaagctgc ctgctttaaa atatctccat gatgccgatt tcttaacctt aaagggagag
     5221 atccgcggtt cagccggatc actggatgag gccgattcca tggcgcttct cctgatctcg
     5281 aaagaccgga aaaaacacgt catgaacctg cccgtaaacg gcgatggaaa gttcaatcag
     5341 cggggattat atttctatga ttccctccag gtcgtttacc ggttcaacca tctttccaaa
     5401 ataaacaaag gaacagagat cagcttattt tccaacctgt tgccgccctt aacgcccgcc
     5461 agggcagaag agccaagcta ttcctggatg aaagttccgg atgtggtatt ggaaaaggaa
     5521 tttaacggaa gcattatgga gaccagcgac tattccgttc cttccgatga catgagttat
     5581 atcgttacgc cgaaacagga ggctcagaaa ggagcttcgg aaacggctgc ccactatctc
     5641 agcaccatgt tcgcggacct ccattttccg gcaaccttaa aagatcatgc aaccggcgat
     5701 gccaggctga cctcctattc aacgggtggc gcggcttccg tacgggctaa tgtgaatatt
     5761 tccattgacg gcgttcccgt tgcgatggat aatttgaaat ctgtgagcat gaaggaagtg
     5821 ttgttcatca agtttgttcc caaaaccagt ccgaaggcgc ttcccacgct ggctatttcg
     5881 agccggcagg cagagcagca aagccttatc atgggaaaca aaacgggatt cgcagtggtt
     5941 accggatata cggctgtccg ggaattttat gcaccccatt atactgataa gatatcggat
     6001 tacgcggctt ctgatttccg ttccacatta tactgggacc cggaagtgaa actggacagg
     6061 tcccaccgga aggtgaaact ggtattctat aataatgata tcacgaccaa gttccgcatt
     6121 gtaattgaag gaatgaacca ggaaggaaag ctaacccgga tcgaagaatt aattaagtaa
     6181 aacctctatc aaaccataaa aacccaaacc catgttaagc gtagaattta cctttagtgt
     6241 gcttgccctg atcggaatta ttgccggatc cctgctgacc gggttccttt taagaagccg
     6301 tcagctccag aaaaagcaat ttaaaatctc tgagctcagg aaagaaattg tttacaatca
     6361 cgcacagata ctggaattgc agaaagagta tgtaatgctt gataagacaa taaagggaaa
     6421 catggctccc gtactgccga tgaaacaggt ggttgccgaa aaaaaggaaa tggctttata
     6481 aggcggaagc gaatcgctta tgccgaatga gcgggcgctt gttctgtcct gatcatttcc
     6541 cggtaaagga gggcattcgc ttttcaaaaa aagcggtgac gccctctttg aaatcccgcg
     6601 tatggcctgc ctgaccctgt aagttttttt ctgtttccag ctgctggtcc agcgtgttgg
     6661 taaaggattg ctgcagggca ttccgggtaa gcattagcgc cgttgtcgga agcatggcca
     6721 gcgtgccggc gatctgagcg gccgattcct caaaccggtc atcctcaaaa catttgtaga
     6781 tcattccatt ctgctctgcc tcggcggccg ttattttatc tcccagcatc atgaaagccg
     6841 ttgctttctg aaggccgacc agccgcggca ggaaataggt tcccccgcaa tccgggatca
     6901 ggccgatttt tgaaaaggcc tgcacaaagg acgcggaggc cgtcgccacc acgatatcac
     6961 aactcaacgc aatattggcg ccggcgccgg ccgctattcc gttcaccgcg caaagcacgg
     7021 gtttccggat cgttctcaac aggcggataa ccggattgta atattcctca ataacttctt
     7081 caaataccgg tagtttccca ccggggaact cactcaggtc ctggccggaa gaaaaggccc
     7141 ggccgttggc ggtgagtagt acgctgcgga tggcatc
//
LOCUS       DASYCU010000418         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4586322, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000418 DASYCU010000000
VERSION     DASYCU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4586322"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VGM24_08455"
     CDS             complement(<1..325)
                     /locus_tag="VGM24_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY4155446.1"
                     /translation="MKTSTWFGLLMLLAISVPLRAGDDLCGVKNTAFQANESITYRVY
                     YTLAGVYIAAGEATFTSVLDNLNGKPVYHITGVGKTYSFYDNFFRVRDKYETFIDTGT
                     LQPYKF"
     gene            complement(322..1308)
                     /locus_tag="VGM24_08460"
     CDS             complement(322..1308)
                     /locus_tag="VGM24_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155447.1"
                     /translation="MALNKNIKLILNYAAGPLLLIFLFYSVSQQLQRQTGWKASVQQI
                     MNAFSGGRQWRLYLMMALMWLNWGLEAAKWQVSILPLQQLGFLRAFKAVLAGACIASF
                     TPNRVGEYFGRMLYMAEGKKVQSVAPAIVCSMGQMLVTLLAGSAGLFLFLVFTPAYPV
                     VWISPPYTRIFLLIAVLSALVLATLYFRVGPLVSLINRIFKKYVKSFSIPQHFSNRML
                     FKILLLSLLRYGVFLLQYFLLFSLFRLNISVLHVFMGVSVMFVVMAVIPSFTILTDLA
                     LRWEAGIRIFQVYTLNTAGILAVSLGIWIVNLIIPALIGSLLILRIKLFSSK"
     gene            1329..1886
                     /gene="ruvC"
                     /locus_tag="VGM24_08465"
     CDS             1329..1886
                     /gene="ruvC"
                     /locus_tag="VGM24_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY4155448.1"
                     /translation="MQKNSKIILGIDPGTLLMGYGLISVEGNQMQLIEMNTLHVPARL
                     DAYQRLELIFFTVQQLIHTYRPTDFAIEAPFFGKNVQSMLKLGRAQGVAIAAAMQSGI
                     PVTEYAPRKIKQSITGNGNAGKEQVWKMLQQILQIQRETEYLDASDALAVAICHHFQG
                     TLPASKEKKINGWGAFVAKNPGRVI"
     gene            complement(1915..2310)
                     /locus_tag="VGM24_08470"
     CDS             complement(1915..2310)
                     /locus_tag="VGM24_08470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY4155449.1"
                     /translation="MTIFSKIINGDIPSYRIAEDDFFLAFLDINPLREGHVLVIPKTE
                     TDKFFDLKEEYLSRILVFARPIARAIEKSFPCDRCGIAVIGLEVPHAHMHLVPIDHID
                     DLNFRKPKLKLTEEQFRRVQEKIIAHLGR"
     gene            complement(2312..2785)
                     /gene="greA"
                     /locus_tag="VGM24_08475"
     CDS             complement(2312..2785)
                     /gene="greA"
                     /locus_tag="VGM24_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY4155450.1"
                     /translation="MSEITYLTKEALKNLKAELQKMKSVDRPAASKAIAEAREKGDLR
                     ENAEYDAAKESQGLLEAKIQRLEADLANVRILEEGNIDTSKVSILTRVTVTNLNNKKQ
                     STYHLVSEKEADLKQGKISVSSPIGKGLLGKQIGEVTEVDVPAGKLKFKVENITL"
     gene            complement(2990..>3334)
                     /locus_tag="VGM24_08480"
     CDS             complement(2990..>3334)
                     /locus_tag="VGM24_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4155451.1"
                     /translation="DVVSLHVPLTGETLHLANDQFFNSFRRAPYFINSCRGKVQDNAA
                     VIRALEHRKISGAALDVLENEKLDQYTVAEKEQLDRLLSFDNVLLTPHIAGYSQEAFY
                     KMAKVVLDKLGL"
BASE COUNT          920 a          824 c          759 g          831 t
ORIGIN      
        1 taaacttata gggttgaagt gtgccggtat cgataaaagt ctcatattta tcgcggacac
       61 ggaaaaaatt atcgtaaaag gaataggttt tccctacgcc ggtaatatgg tatacgggtt
      121 tgccattcag gttgtccagc acgctggtaa aagtggcttc gcctgctgca atataaaccc
      181 ccgccagggt atagtaaacc cggtaggtta tggattcatt cgcctgaaaa gcggtattct
      241 tcaccccgca taaatcatct cccgcccgca acggaaccga aatagccaac aacatcaaaa
      301 gtccgaacca ggtagatgtt ttcatttgct actgaatagt ttgatcctta aaattaataa
      361 actcccgatc aaagcaggta taattaaatt gacgatccag atacccaggg ataccgcaag
      421 tattccggca gtattcaatg tatatacctg gaatatccgt atccctgctt cccatcgcag
      481 cgccagatct gtcagaatcg taaaggaagg aatgaccgcc atcaccacga acatcacact
      541 aacgcccata aatacgtgta acacggaaat gttgagtctg aaaagggaga acagaagaaa
      601 gtattgcaaa aggaataccc cataccggag cagtgataat aaaagaattt taaacagcat
      661 gcgattgctg aaatgctgcg gaatcgaaaa cgatttaaca tattttttga atatccggtt
      721 gatcaggctg accagggggc caacccggaa atataaggtt gccagcacga gggcggacag
      781 gacggcaatc agcaagaata tccgggtata cggaggcgaa atccagacca cgggataagc
      841 cggcgtaaaa accaaaaaaa ggaatagtcc agctgaaccg gccagcaggg taaccagcat
      901 ttgccccata ctgcaaacaa tagccggcgc aaccgattgg acttttttcc cctctgccat
      961 atatagcatt ctcccaaaat attcccctac ccggtttggc gtgaacgagg caatacaggc
     1021 tccggccaga acagccttga atgcccgcaa aaagccaagt tgctgaagcg gcagaatgga
     1081 gacctgccac ttggccgctt ccagccccca gttcagccac atcagggcca tcatgaggta
     1141 aagcctccac tgccggcctc cgctgaatgc gttcattatt tgctgaaccg aagccttcca
     1201 tccggtttgc cgctgtaatt gctgcgacac gctgtaaaaa agaaatatca gaagcagagg
     1261 tcccgctgcg tagttgagga ttaatttgat atttttgttc agcgccacat tcaaaaatag
     1321 ccttcctctt gcaaaagaat tccaaaatca tactgggcat agatccgggg acgctcctaa
     1381 tgggttatgg attgatttcc gtggagggga accagatgca gcttatcgag atgaatacac
     1441 tgcacgtgcc ggccagactg gatgcttacc aacgcctgga gctcattttt ttcacagtac
     1501 aacagctcat tcatacttac cgtccaaccg attttgccat tgaagccccg tttttcggaa
     1561 agaatgtaca gagcatgctt aaactcggcc gcgcgcaggg agtggccatc gcggcagcta
     1621 tgcagtccgg cattccggtt acagaatatg ctccccgtaa gatcaagcaa tccattacgg
     1681 gaaatggcaa tgcgggcaag gaacaggttt ggaagatgct tcagcagatt ttacagatac
     1741 aacgggaaac ggaatacctg gacgcgtccg atgcgctggc ggtggccatt tgtcaccatt
     1801 tccagggaac ccttccggct tcaaaagaaa aaaaaataaa cggctgggga gcgtttgttg
     1861 caaaaaatcc gggaagagta atttagagac cggtaaaagg ggaattgagc cgtttcatct
     1921 ccccaaatga gcaatgatct tctcctgtac gcgcctgaac tgttcttccg tcaattttaa
     1981 ttttggtttc cggaaattca ggtcatctat atgatcgatc ggaaccaggt gcatatgggc
     2041 gtgcggcact tccaatccga tcaccgcgat cccgcagcga tcgcagggaa atgatttttc
     2101 tattgcccgg gcaatgggcc gggcaaagac caggatccgg gaaagatatt cttccttcag
     2161 gtcgaaaaac ttatccgtct ctgttttcgg gataaccagc acatggcctt ctctcaaggg
     2221 attgatatca agaaaagcca ggaaaaaatc atcttctgcg atgcggtagc tggggatatc
     2281 cccattgatt atttttgaga aaatagtcat tttacaaagt aatgttctct accttgaatt
     2341 tcagcttccc tgccggaaca tccacttccg ttacttcacc gatctgcttt ccgagcaatc
     2401 cttttccgat cggggaagaa acagatattt taccctgctt gaggtctgct tccttctcag
     2461 ataccaggtg ataggtggat tgttttttat tgttcagatt cgtcaccgtt actcttgtca
     2521 gaatagaaac tttactcgta tcaatattgc cttcctccag gatgcgtaca ttcgcaagat
     2581 ccgcttccag ccgctgtatt ttcgcctcca gcaatccctg ggattctttt gcggcatcat
     2641 attcggcgtt ttcacgcagg tcacccttct cccgggcttc ggcaatagct ttggaagccg
     2701 ccggccggtc caccgatttc attttctgaa gttcagcctt aagatttttc agggcctctt
     2761 ttgtcagata agtgatttca ctcataaatc ctccttattg tttaaggtaa aagaaaaaaa
     2821 atacaacgcc ctttttggag gcgttgcatt gctgcaaaga taaggaatcc aattgcaaaa
     2881 gattattaca gcgggaacat tttggcggaa ggcccgcctc cacaccgggg tgattgggtg
     2941 ctggttactc ccgtgtaaaa tattgattcc cagctgattc agagccgact tataacccca
     3001 gtttgtccag cacaaccttg gccatcttat aaaaggcctc ctggctgtag cccgcaatat
     3061 gcggggtcag caatacatta tcgaaggaca gcaaccggtc cagttgctct ttttcagcaa
     3121 cagtatattg gtcaagtttt tcattttcaa gcacatccag cgcagcaccc gaaatcttcc
     3181 ggtgttccag tgcgcggata accgctgcat tatcctgaac cttaccccgg cagctgttaa
     3241 taaaataagg cgcccgccta aaggaattaa agaactggtc attggccagg tgcagcgttt
     3301 ctccggtcag gggcacatgc aggctgacca catc
//
LOCUS       DASYCU010000419         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3860233, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000419 DASYCU010000000
VERSION     DASYCU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3860233"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGM24_08485"
     CDS             complement(<1..263)
                     /locus_tag="VGM24_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155452.1"
                     /translation="MKRLIPFLILPFVFSCKNSKNDPDVSGISVNAKIERFDQAFFAL
                     DSNHIQDGLLRLNHEFPYFLNDFTVNILGTGPLSDTSASSFMI"
     gene            387..2195
                     /locus_tag="VGM24_08490"
     CDS             387..2195
                     /locus_tag="VGM24_08490"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY4155453.1"
                     /translation="MKIALVQQNYHIGDFELNTQKIISGIKQARNSGADLVVFSELSV
                     CGYPPRDFLEFEDFINRCYSSLEEIKSHTSDIAVIVGSPERNPVREGKDLFNAAWFLY
                     EKEVKAVVHKTCLPTYDVFDENRYFEPGYEWNLVSFKGKRIALTVCEDIWNLGNNPLY
                     RVCPMDQLMPQQPDFMINISASPFDYDHEEDRRAVVRENVLKYKLPMYYCNAVGSQTE
                     IVFDGGSVIYDGEANLTHQLKVFEEDFCIADTEKKYPPLGAALLPAYAEATTDGALSQ
                     PVFRYDGQRHLPRTGPGLPAKGRQPATADRLPVTDPDQIIPYLTRSNNIKEIHDALIL
                     GIRDYFGKMGFAKAILGSSGGIDSALTLALACEALGNENVRAVAMPSGFSTTHSVADA
                     VQLSKNLGNPFDIIPIQPIYERFLSSLKPVFKDLPFNIAEENIQSRSRGNLLMAIANK
                     FGYILLNTSNKSELATGYGTLYGDMAGGLSVPGDIYKTQVYALARYINRDKEIIPLNI
                     LEKAPSAELRPDQKDSDSLPEYDLLDKILYLYIEKRNGPREIIAMGMDEALVRRVLKL
                     VNSNEYKRNQFCPIIRVSCKAFGVGRRMPIVGKYLG"
     gene            complement(2291..2722)
                     /locus_tag="VGM24_08495"
     CDS             complement(2291..2722)
                     /locus_tag="VGM24_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155454.1"
                     /translation="MMRYSMIFSSAALLFLGFTACKKSDNANSSKTMANISGSYNLAA
                     LTASLGSINYNLYDTLPDCQKDNVIILNADSTAQFVDGGITCDPPGDSTAIWHLSAHA
                     DSLYLGNNATFIQKFDGKNLVVTADRFNGYTGEITITLARH"
     gene            2856..>3365
                     /locus_tag="VGM24_08500"
     CDS             2856..>3365
                     /locus_tag="VGM24_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4155455.1"
                     /translation="MYTADDIKQLNEKIQDQGLFVDRLREEIGHVIIGQQYMLDRLLI
                     GLLSNGHVLLEGVPGLAKTLTIKSLAQTIKGKFSRIQFTPDLLPADVVGTMIYNQQKN
                     EFVVRKGPIFANFLLADEINRAPAKVQSALLEAMQERQVTIGDSSYILQEPFLVLATQ
                     NPLEQEGTYP"
BASE COUNT          907 a          813 c          812 g          833 t
ORIGIN      
        1 cagatcataa aactgctggc gctggtatca gacaaaggac cggtacccag aatatttacg
       61 gtaaaatcgt ttaagaaata aggaaattcg tggttcagtc gcagcagtcc gtcctggatg
      121 tggttggagt ccagcgcaaa aaatgcctgg tcaaaccgct cgatctttgc attgacggat
      181 attccggaaa catcggggtc gtttttgctg tttttacagg aaaaaacaaa aggaaggatt
      241 aagaaaggga ttagtctttt catcaacatg gaaggtaatt ggtggatggc cgatggatat
      301 caggtgcgaa tttacacata taagacggtc aaccattccc catttaccat cctcaaaaat
      361 cccctgctta atagtaattt tacagcatga aaattgcgct ggttcagcag aactatcata
      421 tcggtgattt cgagctcaat acgcagaaga tcatcagcgg cataaagcag gcccggaact
      481 cgggcgcgga cctggtggtt ttttcggagc tttccgtttg cggatacccg ccccgcgatt
      541 tcctggagtt cgaagatttc attaaccgct gttattcatc actcgaagaa atcaaatccc
      601 atacttcgga cattgccgtg atcgtgggct ccccggaacg caatcccgtt cgcgagggga
      661 aggatctgtt caatgccgcc tggtttcttt atgaaaaaga agtgaaggcc gtggtacata
      721 aaacctgtct cccgacctac gatgtttttg atgaaaaccg ttatttcgaa ccgggttatg
      781 agtggaacct ggtttccttc aaaggcaagc gcatagccct gaccgtttgt gaagacatct
      841 ggaacctcgg caacaatccg ttgtaccggg tttgtccgat ggaccagctc atgccccagc
      901 agcctgattt catgatcaat atttccgcct cgcccttcga ttatgatcat gaagaagacc
      961 gtcgcgcggt ggttcgggag aatgtgctga aatacaaact gcccatgtat tattgcaatg
     1021 cagtgggctc gcaaacagaa atcgttttcg acgggggcag tgtcatatac gatggggaag
     1081 ccaaccttac gcatcagctt aaagtattcg aagaggattt ctgcatagca gataccgaaa
     1141 aaaaatatcc gcctctgggc gccgctcttc ttcctgccta cgcggaagct acgacggacg
     1201 gagccctaag ccagcctgtg tttcgttacg acgggcaacg gcacctgcca aggacaggtc
     1261 ccggactgcc ggccaagggg cgtcagcccg caacggccga ccggctgccc gtgacagatc
     1321 cggaccaaat catcccctat cttacacgca gcaacaacat caaagagatt cacgatgccc
     1381 tcatcctcgg catccgggat tattttggaa aaatgggatt tgccaaagcg atcctcggca
     1441 gcagcggggg aatagacagt gcgttgacgc tggccctggc ctgtgaagcc ctgggaaatg
     1501 aaaatgtgcg cgcggtagcc atgccctccg ggttttcaac cacgcattca gtggccgatg
     1561 ccgtccagct ttcgaaaaat ctggggaatc cttttgatat cattcccatc cagcccattt
     1621 atgagcgttt tctttcttcc ttaaaacctg tttttaaaga cctaccgttt aatattgcgg
     1681 aagaaaatat ccagagccgt tcgcggggaa atctgttgat ggccatcgcc aataagttcg
     1741 ggtacattct gctgaacacc tccaataaaa gtgagcttgc aacgggttat ggaactttat
     1801 acggagatat ggccggggga ctcagtgttc cgggcgacat ctataaaaca caggtctatg
     1861 cgctcgcccg gtatatcaac agggacaaag aaattatccc gctcaatatt ctggaaaaag
     1921 cgccctctgc ggagttgcga cccgatcaga aagacagcga cagtcttccg gaatacgatc
     1981 tcctcgataa aattttatac ctctacatcg aaaagagaaa cggacccaga gagatcatcg
     2041 ccatgggcat ggacgaagcc ctggtcaggc gcgtgctcaa actcgtgaac agcaacgaat
     2101 ataagcgcaa ccagttctgt cccataatcc gggttagctg caaagctttc ggtgtgggga
     2161 ggaggatgcc gattgtgggg aaatatttgg gttgaagctg tgggaatatc gtggatggtc
     2221 tttacatgcg aatccccacc cgattcatcc accatctacc atccaccatc caccatccac
     2281 catccacgta ctagtgcctg gccagtgtaa tcgtaatttc tcccgtgtac ccgttaaaac
     2341 gatccgcggt gaccaccagg ttttttccgt cgaatttttg gatgaaagtt gcattatttc
     2401 ccagatagag tgagtctgca tgggcagaaa gatgccagat ggctgttgaa tcaccgggcg
     2461 ggtcgcaggt gattcccccg tccacgaact gggccgtact gtctgcattc agtataatca
     2521 cattatcctt ttgacaatcc ggcagggtat catataaatt atagtttatg ctgcccagac
     2581 ttgcggttaa tgcagccaga ttataagatc ccgaaatatt ggccatggtt ttggaactgt
     2641 ttgcattatc acttttttta cacgcagtaa accccagaaa aaggagagcc gctgaagaga
     2701 aaatcattga ataacgcatc ataatcaaag ttttaatgat ataattcttt tgtaactatg
     2761 tatgttaaat tttattgtgc gcctctctga agttttaaca agtgaggggg ttcttattaa
     2821 cgaattcgta ttttgcagcc taatttttta tcaatatgta caccgcggat gatattaaac
     2881 agctaaatga aaagatccag gatcagggat tgtttgttga ccggctccgg gaagaaatcg
     2941 gtcatgtaat catcgggcaa caatatatgc tggaccgttt gctaatcggg ctgctcagca
     3001 atggccatgt gctgctggag ggcgtgcccg gactggcgaa gaccctcacc atcaaatcac
     3061 ttgctcaaac cataaaagga aaattcagcc gcattcagtt tacacccgat ctgctgccgg
     3121 ccgacgtggt gggcaccatg atttataatc agcagaagaa cgaatttgtg gtgagaaaag
     3181 gacccatttt cgccaatttt ctcctcgcag atgaaatcaa ccgcgccccc gcaaaagtac
     3241 agagcgccct cctggaagcc atgcaggaaa ggcaggtaac cattggagac agcagttata
     3301 tactgcaaga accttttttg gtgctggcca cccaaaatcc gctggaacag gaaggaactt
     3361 atccg
//
LOCUS       DASYCU010000420         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2989852, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000420 DASYCU010000000
VERSION     DASYCU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989852"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VGM24_08505"
     CDS             complement(<1..385)
                     /locus_tag="VGM24_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155456.1"
                     /translation="MLTYLLFVFYLALGTMVLHRILRRKGFPFSLYHTSAALLFKVCL
                     GCLYGYIFLHYYGGDDTWEYFTKSRAETDILLTRPDRFIQEFLPSFSLKATHFNGWSA
                     VVYFVHHFEFWFMIKFLAVLNLFSGK"
     gene            445..1584
                     /locus_tag="VGM24_08510"
     CDS             445..1584
                     /locus_tag="VGM24_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4155457.1"
                     /translation="MIENPPVPKRKKIALVANSAWSVYNFRIDLVRHLISRFDVLVIA
                     PEDEFAAELKKEGCSFLNIDFNNRSENPLRDYFLFRSFKKIYSEQKPDFIFHYVIKPN
                     IYGSLAAAACGIESIAVITGLGYAFARRNWLKKTVSYLYKIALKRTREAWFLNREDAA
                     IFMERRLVEPAKVKILPGEGINPAFFAPVQFRPVARTRAFQFLMSTRLLKSKGVSIYV
                     EAASILKKKNRDIRFELIGFFEEQHPDSITKAEIRYWQKRDYIHYSGFARDVRPFLRQ
                     ADCLVFPSFYPEGIPRCLMEAASMEVPIITSLTRGCKEVVEDGGNGFLCTVNNAAALA
                     EKMEEMMQLSPSQRKAMGKKGRELIIEKFDVQKILLEYDRALEHI"
     gene            complement(1581..2495)
                     /locus_tag="VGM24_08515"
     CDS             complement(1581..2495)
                     /locus_tag="VGM24_08515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4155458.1"
                     /translation="MKIAFIHSRRTFLPELDAYQSFFQSKNIQSVVCRYGEEAASGAD
                     VYWYFMGIYPGAAARGKRIIHEYASASVPPYRKFKDLLKARLNPRPHFRLYLNEYVHQ
                     EIQHRDEIPFGYRDMGISDGFIPDPGAEKKYDFIYVGSFARERKIETLLRVFQEGVMK
                     ERSLLLLGQDYDRLASRYSSSGNIIFRGPVSRREMPAYLATARFAINFIPDREPFNAQ
                     TSTKFLEYAAMQIPIISSNYFWISHFRERYGGRYFFLKEDLSNLSWGLVSSFRYEFPK
                     LQSWHWENQIRSSGILDYLQSVFPGISF"
     gene            2561..>3497
                     /locus_tag="VGM24_08520"
     CDS             2561..>3497
                     /locus_tag="VGM24_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY4155459.1"
                     /translation="MEINVKTAEQLRLTAEEFDLICKKLGRTPNFTELCAFSAMWSEH
                     CSYKNSIKWLKTLPREGKKMLVKAGEENAGLMDIGDGLAVVFKIESHNHPSAIEPFQG
                     AATGVGGIHRDIFTMGARPIASLNSLRFGKLSDSRTQHLLAGVVRGIGHYGNCFGVPT
                     VGGEIYFDECYHTNPLVNAMSVGIVKKGATVSATALGAGNPVFFVGSATGKDGIGGAS
                     FASANITQESVEELPAVQVGDPFQEKKLLEACLEVIATGAVVGMQDMGAAGIICSTAE
                     MSAKGETGMRIDLDKVPARQTGMKAWELLLSESQER"
BASE COUNT          939 a          811 c          940 g          807 t
ORIGIN      
        1 tcttcccgct gaagagattt agcaccgcga gaaatttgat cataaaccag aattcaaaat
       61 ggtggacaaa atagaccacc gcgctccagc cgttgaaatg ggtggctttc agggaaaagg
      121 aaggcaggaa ttcctgtata aaccggtccg ggcgggttaa caggatatcc gtttctgccc
      181 tggatttggt aaaatattcc caggtatcgt ccccaccgta atagtgcaga aaaatataac
      241 cgtataaaca gcccaggcat accttgaaaa gaagggctgc cgaggtatgg tagaggctga
      301 agggaaaacc tttccggcga aggatccggt gaagcaccat cgtacctaag gccaggtaaa
      361 atacaaagag aaggtaggtg agcatctgaa aggtttaccc gattttgttc cccaaatcta
      421 taaatttgaa tttaatatct cctgatgatc gaaaatccgc ctgtcccgaa gaggaaaaag
      481 atcgcgctgg tggcaaacag cgcctggtct gtttataatt tccggataga cctggtgcgg
      541 cacctgattt cgcggtttga cgtgctggtg atcgctccgg aagacgagtt tgccgcggaa
      601 ttaaagaaag aaggctgcag ctttctgaat atcgatttca ataaccgttc ggaaaatccg
      661 ttgcgcgact atttcctttt ccggtcattc aaaaaaatct atagcgaaca aaaaccggat
      721 tttattttcc actatgtgat caaacccaat atttacggct cgctggccgc cgctgcctgc
      781 gggattgaat ccattgccgt gatcaccggt ctcgggtatg cctttgcgcg ccggaactgg
      841 ctgaaaaaaa ccgtttcgta tttatataag atcgccttga aacgtacgcg cgaagcctgg
      901 tttctgaacc gggaagacgc tgccatcttt atggagcgga gactggtgga accggcgaaa
      961 gtgaaaattt taccggggga gggcatcaat cccgcttttt ttgcacccgt gcagttcagg
     1021 ccggttgcca gaaccagggc cttccagttt ctcatgagca cccggctcct gaaaagtaaa
     1081 ggagtgagca tttatgtgga agcggccagc atactgaaga aaaaaaaccg cgatatccgt
     1141 ttcgaactca tcggtttctt tgaagagcag catccggatt ccatcacaaa agcggaaatc
     1201 cgctactggc agaaaagaga ttatatccat tacagcggat ttgcaagaga cgtgcgcccc
     1261 tttctgcggc aggcagattg cctggtattc ccttcctttt acccagaagg cattccgcgt
     1321 tgtctgatgg aggccgcttc catggaagtt ccgatcatca cctcgctgac caggggatgt
     1381 aaagaggtgg ttgaagacgg cgggaatggt tttttatgca ccgtgaataa tgcggcggcc
     1441 ctggcggaaa aaatggaaga aatgatgcag ctgagtccgt cccaaagaaa ggccatggga
     1501 aaaaagggcc gggaactgat cattgaaaaa ttcgatgtgc aaaagatcct cctggaatat
     1561 gaccgcgccc tggaacatat ttaaaatgaa attcccggaa agaccgattg caggtaatcc
     1621 agtatgccgg aagaccggat ctgattttcc caatgccagg actgcagttt tggaaattca
     1681 tagcggaatg aactgaccag gccccagctc aggtttgaaa gatcctcctt gagaaaaaaa
     1741 tatcttcccc cgtatctttc ccggaaatgg gaaatccaga aatagttgct gctgatgatc
     1801 gggatctgca tggcagcgta ttccagaaat ttggtggagg tctgcgcatt gaaaggttcc
     1861 cggtccggaa taaaattgat ggcaaaacgc gctgttgcca ggtaagcggg catttccctg
     1921 cgggataccg ggccgcggaa aataatattc ccggaagaag aataacggct ggccagccgg
     1981 tcataatcct gacccagcaa aagaaggctt cgctctttca taaccccctc ctgaaatacc
     2041 cggagaaggg tttcgatctt tctttcccgg gcgaaggaac cgacataaat aaaatcgtat
     2101 tttttttccg ccccggggtc tggtataaaa ccgtcgctga tccccatgtc gcggtagcca
     2161 aacggtatct cgtcccggtg ttgaatttcc tggtgcacat attcattgag atataaacgg
     2221 aaatgcggac gcggatttaa tctggccttg agaagatcct tgaacttgcg gtaaggcggg
     2281 accgaagcgg acgcgtattc atgaatgatc cgctttcccc gcgcggcggc tcccgggtaa
     2341 atgcccataa aataccagta gacatcggcg ccggaagcgg cttcctcccc gtatctgcag
     2401 acgacagatt gaatattttt tgactgaaag aaggactggt aggcgtccaa ttccggtagg
     2461 aatgtgcgcc tggaatgaat gaaagcaatc ttcatacggt ggtaaaatta tcaaatagat
     2521 taacgcgtgc ttcgtaattt tgctgtttcc caaaacgaat atggaaatta atgtcaaaac
     2581 cgccgaacaa ctccgcctga cggcggagga gtttgacctg atctgtaaaa agctcgggcg
     2641 cacgccgaat ttcacggaac tctgtgcttt cagcgccatg tggagtgaac actgcagcta
     2701 taaaaattca atcaaatggc tcaagaccct tccccgcgaa ggaaagaaga tgctggtaaa
     2761 agccggggag gaaaatgccg ggctgatgga tatcggagac ggcctggccg ttgtctttaa
     2821 aatagaatcc cacaatcatc cctccgctat tgagcctttc cagggagcgg caaccggtgt
     2881 gggaggcatt caccgggaca ttttcaccat gggcgcgcgc ccgatcgcat cgctgaattc
     2941 cctccgcttc ggtaaattgt ccgattccag gacccaacac ctgctggcgg gcgtagtaag
     3001 agggattggt cattacggaa actgtttcgg tgttccgacc gttggtggag aaatttattt
     3061 tgacgaatgc tatcatacca atcccctggt caacgcgatg agtgtgggta ttgtgaaaaa
     3121 aggggcaacg gtttcggcca cggcgctggg tgcgggcaat cccgtttttt ttgtagggag
     3181 cgcaacggga aaggatggca tcggaggggc ttctttcgct tctgcgaata tcacccagga
     3241 aagtgtggaa gaactgcctg ccgtgcaggt gggagatccc tttcaggaaa aaaaattact
     3301 ggaagcctgt ctcgaggtga tcgccaccgg agcggtggtt ggtatgcagg atatgggcgc
     3361 agccggcatc atctgttcca ccgcggaaat gagcgcaaag ggagagacgg gcatgcggat
     3421 tgatctggat aaagtgcctg cgcggcagac gggaatgaag gcctgggaat tattgctcag
     3481 tgaaagccag gaaagaa
//
LOCUS       DASYCU010000421         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_91836, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000421 DASYCU010000000
VERSION     DASYCU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91836"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..>3075
                     /locus_tag="VGM24_08525"
     CDS             326..>3075
                     /locus_tag="VGM24_08525"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY4155460.1"
                     /translation="MKDVQYITNSSPAFIESLYQDFVKDPQSVDPEFKKFFEGFDFAI
                     SQGKNGNSPAATAPSRDGGLKTAEGLDWKKELGAYRLILGYRNKGHLVAKTNPIRPRK
                     DRGANLDIGFFGFTEEDLDKNFFAGNLIGLGTTTLRNILSHLQQCYAYHVGIEFKYIS
                     DQKKIDWLTNEMEKKFLEPLPLQKRQRILDKLNEGVMFEKFLHTKYIGQKRFSLEGGE
                     TTIPAIDAIINIAADHEVQEVVMGMAHRGRLNILANILGKTYEQIFSEFEGTAKVDQT
                     MGTGDVKYHLGFGSELTTPNKKTVHVKLMPNPSHLEAVDPVVVGFARAKADVLYESNY
                     DKILPILIHGDASLAGQGIVYEVLQMCDLKGYYTGGTIHFVINNQIGFTTDFDEARSS
                     DYCTSLAAAVHAPVLHVNGDDPEAAVKCAEIATRYRQEFNADFFVDMVCYRRHGHNEG
                     DDPKFTQPTMYALIDQHPNPREVYTQFLIENGEADAKDLAKDMEKKFWNDLQERLDEV
                     KQKPLPYKYQQPELWWRSLRRATTEDFERSPDTAISEAALKKIFDAMMKWPETFTPIR
                     KVEKLLKDKIKLFNEEQKLDWSSGELLAYGSILLDGKDVRMSGQDVRRGTFSHRHAVL
                     RDEKTDESYNRLSRIPEAKGKCRIFNSLLSEYGVLGFEYGYALANPNALVLWEAQFGD
                     FGNGAQIMIDQFIAAGEQKWQRMTGLVLLLPHGYEGQGPEHSSARIERFLQMCAELNL
                     VITNITTSANYFHAMRRQLAWPFRKPLVNFSPKAYLRYPGSYSRISEFTSGRFLEVID
                     DQYAGEPSKVKKVLFCSGKIYFELAERQVKDNRKDIAIIRLEQLYPLPTAQLDELYKK
                     YLNATWFWIQEEPLNMGAASFLQMNLKNINYGFISRQPGASPATGYAKVHAQEQAEII
                     ETA"
BASE COUNT          815 a          772 c          812 g          676 t
ORIGIN      
        1 tcttttcctt gatcactttc agatcgcgga tggcattacg ggtagtcttg ccataatacc
       61 ggttccggtc cctgcgtttt gttgctgccc ggacgtcctt tttcgttgcc ttgtgattcg
      121 ccatgaattc gaaaatttgg acggcaaagg taggaaaacc acggaaatat tgaaaattaa
      181 tcaggaactt tcttttgccg atatttgcag gacgattcag cagcccattt cggataaatc
      241 tttcgggata gaattttaaa aattgcctcc ggctgatgaa cttgcatacc ggctgaaaac
      301 cggatgatcc aaagataaat ttacgatgaa agacgttcag tatattacca attcctctcc
      361 ggcctttatt gaaagcttgt accaggattt tgtaaaagat ccacaaagtg ttgatcctga
      421 gtttaaaaaa tttttcgaag ggtttgattt cgccatttcc cagggtaaga acggaaattc
      481 cccggcggct accgctcctt cgcgggatgg gggactgaaa acggccgaag ggctggactg
      541 gaagaaggaa ctgggggcct accgcctcat cctcggctac cgcaacaagg gccacctggt
      601 ggccaagacc aatccgatcc gccccaggaa ggaccggggc gccaacctgg acatcggctt
      661 tttcgggttc accgaagagg acctggacaa gaactttttt gccggaaacc tgatcggact
      721 gggcacgacc actctccgga atattctcag ccatctccag cagtgctatg cctatcatgt
      781 gggcatcgaa ttcaaataca tcagcgatca gaaaaagatc gactggctca ccaacgagat
      841 ggagaagaag tttctggaac ccctgccgct tcagaaaaga caacgcatcc tggataaact
      901 gaatgaaggg gtgatgtttg aaaaattcct tcacaccaaa tacatcggac agaaaagatt
      961 ttcactggaa ggcggggaaa ccaccattcc cgccattgat gccattatca atatcgctgc
     1021 tgatcatgaa gtgcaggaag tggtgatggg catggcccac cggggccgcc tgaatatcct
     1081 cgcgaatatt ctcggcaaaa cctatgaaca gatcttcagc gagtttgaag gcactgccaa
     1141 agtggatcag accatgggca ccggcgatgt gaaataccac ctgggcttcg gaagcgaact
     1201 gaccacgccc aataaaaaaa ccgtgcatgt aaagctcatg ccgaaccctt cccacctgga
     1261 agcggtggac cccgtcgtgg tcggttttgc ccgggccaag gcagatgtat tgtatgaaag
     1321 caattacgat aaaatattac cgatattgat tcatggggac gcctccctgg ccgggcaggg
     1381 gatcgtttat gaagtattgc agatgtgcga cctgaaagga tattataccg gggggaccat
     1441 ccattttgtg atcaataacc agatcgggtt caccacggat ttcgacgaag cccggagttc
     1501 cgattactgc acttcgctgg ctgcggccgt gcatgccccc gttcttcacg tgaatgggga
     1561 cgatccggaa gcggcggtga aatgcgcgga gatcgccacc cgctaccgcc aggaattcaa
     1621 tgcggatttt tttgtcgata tggtctgtta ccgccggcat ggccacaacg aaggggatga
     1681 tccgaaattc acgcagccca ccatgtatgc attgattgat cagcacccca atccccggga
     1741 agtatatacg cagttcctga tagagaacgg ggaagcggat gcaaaggacc tggccaagga
     1801 catggagaaa aaattctgga acgatctgca ggaaagactc gatgaagtga aacaaaaacc
     1861 gcttccgtat aaataccagc agccggaatt gtggtggaga agcctgcgcc gggccacgac
     1921 ggaagatttt gaaagatctc cggataccgc gatctcggaa gccgccctga aaaagatctt
     1981 cgacgcgatg atgaaatggc cggagacctt tacgcccatc cgcaaagtgg agaaactgct
     2041 gaaagataaa atcaaattat ttaatgaaga acaaaagctg gactggtcct ccggggaact
     2101 gctggcttac ggaagcatat tgctggatgg gaaagatgtg cggatgagcg ggcaggatgt
     2161 gcgcaggggc acattttccc atcgtcatgc ggtgctgcgg gatgaaaaaa ccgacgaatc
     2221 ctataaccgg ctgagccgta ttccggaagc caaggggaag tgcaggatct ttaattccct
     2281 gctcagtgaa tacggcgtcc tgggttttga atatggatac gcgctggcaa atcccaatgc
     2341 cctcgtgctc tgggaagcgc agttcggcga ttttggaaac ggcgcccaga tcatgattga
     2401 tcagttcatt gcggcgggag aacagaaatg gcagagaatg acgggcctgg tgctcctgct
     2461 gccccatgga tatgaagggc agggccccga gcacagcagc gcgcgcatcg agcgctttct
     2521 gcagatgtgt gccgagctta acctggtgat cacgaatatt acgacatcgg ccaattactt
     2581 ccacgccatg cgccgtcaac tcgcctggcc cttccggaag cccctggtta atttttcgcc
     2641 caaggcttac ctgcgttacc ccggttccta ttccaggatc tccgaattta ccagcggacg
     2701 gttcctggaa gtgatcgacg accagtatgc cggggaacca tccaaagtga aaaaagtgtt
     2761 gttctgttcc gggaagatct atttcgaact cgcggaaagg caggtgaaag acaaccggaa
     2821 agatattgcg atcatccggc tggaacagtt gtatcccctg cccacggcgc aactcgatga
     2881 attgtataaa aaatacctga acgccacctg gttctggatc caggaagagc cgctcaatat
     2941 gggcgccgct tccttcctgc agatgaacct gaaaaatatt aattacggat ttatcagccg
     3001 ccagccgggc gcttcccctg ccaccggcta tgccaaagtg cacgcccagg aacaggcaga
     3061 gatcattgaa accgc
//
LOCUS       DASYCU010000422         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_816081, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000422 DASYCU010000000
VERSION     DASYCU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816081"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VGM24_08530"
     CDS             <1..383
                     /locus_tag="VGM24_08530"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY4155461.1"
                     /translation="IIRQFNEFNPVDTLHVNGEATQGENIADLGGLLLGLDAFSKTEA
                     FKKGEKIGGLTPMQRYFLGYAYGWLYSERKEVLASQLMTDVHAPARERVNGPMANIPA
                     FYEAFGVKPGDKMYRPDSLRVDIW"
     gene            461..1438
                     /locus_tag="VGM24_08535"
     CDS             461..1438
                     /locus_tag="VGM24_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEY4155462.1"
                     /translation="MISKTGFCIRPFPRNLLGVAVLFFFVSGTRADYSRSPQDTASIE
                     GRWDITVNNSGRSFPSWLEVRHSGLNTLVGRFVGGGGSARPISRVNFSNGKMSFSIPP
                     QWEKENKDLSVEGTLQGDRLTGTMIAPDGKEYSWTGLRAPTLRREKEPVWGDPVVLFD
                     GKDLNGWHPLGKVNQWQAESGVLRSPKSGSNIATDQRFTDFKLHIEFRYPKGSNSGVY
                     LRGRYEVQIEDSKGLEPMDNLLSGVYGFLTPSEMMAKDAGEWQTYDITLVGRLVTVAL
                     NGKTVICNEEIPGITGGALDSNEGEPGPIYLQGDHGPIEFRNIEITPAR"
     gene            1461..>1840
                     /locus_tag="VGM24_08540"
     CDS             1461..>1840
                     /locus_tag="VGM24_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY4155463.1"
                     /translation="MILNKRHFSPRCSFRYLVLISFCLFNFSALRSQTTDPVRFKKII
                     LTKDFVSEGVTTADVNRDGKTDILAGAFWFEAPAWTRHEISKSLIFSPDTAFSNSFLN
                     FSLDVNQDGWTDLVRVGFPGKEVV"
BASE COUNT          473 a          412 c          509 g          446 t
ORIGIN      
        1 agatcatccg gcagttcaat gaattcaatc cggtggatac cctgcacgtg aatggagaag
       61 caacccaggg agaaaatatc gctgacctgg gcggcctcct gctgggactg gatgcttttt
      121 caaaaacaga agcttttaag aagggagaaa agatcggcgg ccttacgccc atgcaacgtt
      181 atttcctggg atatgcgtat ggctggttgt atagtgaaag aaaggaagtc ctggcgtctc
      241 aattgatgac cgacgtacat gcgccggcca gggaaagagt gaacggcccc atggccaata
      301 tccctgcctt ctatgaagcc ttcggcgtaa aaccaggcga taaaatgtac cggccggaca
      361 gcttgcgggt ggatatctgg tgatccggat taaccggaag aaaagcagag aacaaccagt
      421 tgattgtttt taagtaaatt gggggtaaga aaaattccat atgatcagca aaacaggatt
      481 ttgtatccgt ccctttccca ggaacctcct gggcgtggcc gttttatttt ttttcgtctc
      541 cggcacccgg gcggattatt cccgttctcc acaggatacc gcttccatcg aagggcggtg
      601 ggatataacg gtgaataatt ccgggagatc atttccttcc tggctggaag tgcggcattc
      661 aggattgaat acgctggtgg gccgctttgt tggcggtggt ggaagtgccc gtcctatttc
      721 gagggttaac ttcagtaatg gaaagatgag tttttcgata ccaccgcaat gggaaaagga
      781 aaataaggat ctgtctgttg aaggaacgct gcagggggat cgtttaacgg gaaccatgat
      841 cgctccggat ggaaaggaat attcctggac cggccttcgg gcgccaacac tccgcaggga
      901 aaaggaaccc gtctggggtg atccggtcgt tctttttgat ggaaaggacc tgaatggctg
      961 gcacccgctc ggtaaagtga atcaatggca ggcagaatcc ggagtgcttc gcagcccgaa
     1021 atccggttcc aatattgcga ccgatcaacg ctttacagat tttaaactgc atattgaatt
     1081 ccggtatccg aaaggaagca acagcggggt ttacctgaga gggcgttatg aagtgcagat
     1141 tgaagacagc aagggactcg agcctatgga taatctgctg agcggcgttt atggatttct
     1201 gacacccagt gaaatgatgg caaaggatgc cggagaatgg caaacctatg atatcacgct
     1261 cgtcggaaga ttggtgaccg ttgccctgaa tggtaaaaca gtcatctgta acgaggaaat
     1321 accggggatc accgggggtg cactcgacag caatgagggc gagcccggac cgatttatct
     1381 tcagggggac cacggtccta ttgaattccg aaatattgaa atcactcccg ccaggtagta
     1441 ctggccccga taagatagaa atgatattga acaaacggca tttcagtccc cgctgttcct
     1501 tccggtatct cgttctgatc agcttttgtt tatttaactt ttccgccctg cggtctcaaa
     1561 ctaccgatcc ggtaaggttt aaaaagatca tcctgacaaa agactttgtt tcggaaggag
     1621 tgacgactgc cgatgtcaac cgggatggaa aaaccgatat cctggcgggg gctttctggt
     1681 ttgaagcgcc ggcctggacc cggcatgaga tttcgaaaag tctgatcttt tctcccgata
     1741 ccgccttcag taattcattt ttaaatttca gtctggatgt aaaccaggat ggctggacag
     1801 acctcgtgcg ggtcggtttt cccggaaagg aagtcgtgtg
//
LOCUS       DASYCU010000423         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3861022, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000423 DASYCU010000000
VERSION     DASYCU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3861022"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VGM24_08545"
     CDS             complement(<1..425)
                     /locus_tag="VGM24_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY4155464.1"
                     /translation="MIDLRSDTVTRPTPGMLQAMMQAQVGDDVFREDPTINALEKIVA
                     DLFGTEAALYCSSGTMSNQIAIKCHTRPGDEIICEREAHVYIYEGGGIAFNSGSQVKA
                     IAGDRGRMKAEQIPESVNPDDVHKARTSLVCLENTSNRGG"
     gene            574..789
                     /locus_tag="VGM24_08550"
     CDS             574..789
                     /locus_tag="VGM24_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY4155465.1"
                     /translation="MDKLSLKGNWNMLKGKLKQSYANLTDDDLKFEEGKEDELIGRLQ
                     KKTGQTKEELNKWFKTQLDSSKKPHHA"
     gene            942..2034
                     /gene="prfB"
                     /locus_tag="VGM24_08555"
     CDS             join(942..1001,1003..2034)
                     /gene="prfB"
                     /locus_tag="VGM24_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131330388.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY4155466.1"
                     /translation="MTQEKLNDMRSRLSGLRRFLDVTNRQIKIEEEKQLTGSPGFWND
                     NERATGILKNIKVNEYWVKLYEAVETAIEDFAVLLDFWKAGEATEEETKEAYQKALNL
                     IDEAEFKSTLNQPEDELPAVLQINSGAGGTESQDWAEMLMRMYRMYGEKQGWSVSELD
                     YQDGEAAGIKSVTLQFDGPFAYGFLKAESGVHRLVRISPFDSNARRHTSFASVYVYPL
                     IDDSIEIEVSPADVEWAFFRSGGAGGQNVNKVETAVRLTHKPSGIIVECQQARTQGEN
                     REKAMAMLKSRLYEEEIRKRDAIKNANNASKKKIEWGSQIRSYVFHPYKMIKDHRTDF
                     ETGNVQPVMDGELDGFIKAYLMTEKETAN"
     gene            2062..>2713
                     /locus_tag="VGM24_08560"
     CDS             2062..>2713
                     /locus_tag="VGM24_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4155467.1"
                     /translation="MNLGYFHYAMPSNEPVLNYAPGSPEKISLKKTIREIKGQEHDIP
                     MIIGGKEVRSGNKIKMHPPHEIAHNLGSFHRAGPDHIQQAIQAALAAKPAWEALNWEN
                     RAAIFLKAADLLAGKYRSYMNAATMIGQSKNPYQAEIDGVCELIDFLRFNTHYLSEIY
                     RQQPNSSPGVHNRLEYRPLEGFVLAITPFNFTAIGGNLPSSAALCGNTVVWKPADTQ"
BASE COUNT          783 a          635 c          672 g          623 t
ORIGIN      
        1 cctcccctgt tggaagtatt ctccagacaa acaagagaag tcctggcttt gtgcacatca
       61 tccggattca ccgattccgg aatctgttcc gctttcatcc tgccacggtc gccggcgatg
      121 gcctttacct gcgatccgga gttaaatgca atgccccctc cttcataaat atatacatgg
      181 gcctcccgtt cacaaattat ttcatcaccg ggccttgtat ggcatttgat ggcgatctga
      241 ttggacattg tcccgctgct gcaatataac gcggcctccg taccaaacag gtccgccacg
      301 attttttcga gtgcgtttat cgttgggtct tcccggaaca catcatcacc tacctgcgcc
      361 tgcatcatgg cctgtaacat accgggtgtc ggacgggtga ccgtgtcgga tctcagatca
      421 atcataaaac ggggaaaatt taggcattgt taaataaatc agggattgtt ttccccatta
      481 aaagtaatac agaatgaaat gaactgttaa tgaaattccg gatgttccgg atggcatgaa
      541 gattgagcca ttgtctttat aaaaccaatt attatggaca agttatcact aaaaggcaat
      601 tggaatatgc tgaaaggaaa attaaagcaa tcctatgcca atctaacgga cgacgatctg
      661 aaatttgagg aaggtaaaga agacgaattg atcggaagat tgcaaaaaaa gacgggacag
      721 acaaaagaag agctgaataa atggttcaaa acacagctgg attcctcgaa gaaacctcat
      781 cacgcgtaag gccaacgccc aacgcgaaac gccggttgaa atacgggttt ctttcttttt
      841 aaattaacaa aaggacgaaa actgtttctc accggcgttt cgcgttttgc cttcggcgcc
      901 gggcatccgg cgtttcgtgt ttttccgtaa cttgcgcccc tatgacacag gaaaagctaa
      961 acgatatgag gtcccggctg agtggactga ggaggtttct ttgacgtcac taaccgacag
     1021 atcaaaattg aggaagagaa acaactgacc ggaagccccg gattctggaa cgacaatgaa
     1081 cgtgcaacgg gcattctcaa aaacattaag gttaacgagt attgggtaaa actctatgag
     1141 gcggtggaaa cggccataga ggattttgcc gtattgcttg atttctggaa agcgggagaa
     1201 gccaccgagg aagaaacaaa agaagcttac cagaaggccc ttaacctgat agacgaagcc
     1261 gagtttaaga gtacgctgaa ccagccggaa gatgaacttc ctgccgtttt gcaaatcaat
     1321 tccggtgcgg gcggaacaga aagccaggat tgggccgaga tgctgatgcg tatgtatcgc
     1381 atgtatggcg aaaaacaagg ctggtctgtt tctgagctcg attaccagga tggcgaagcc
     1441 gccggaataa aaagcgttac cctccagttt gacggtcctt tcgcctatgg atttctgaag
     1501 gccgaaagcg gggttcaccg gctggtgcgc atctccccat tcgacagcaa tgcgcgccgt
     1561 catacttcct ttgcgtcggt atacgtatat cccctgatcg atgacagtat tgaaattgaa
     1621 gtgagtcccg ccgacgtgga atgggcgttt ttcagaagtg gcggtgccgg cggacaaaat
     1681 gtaaacaaag tggaaacggc cgtgcgtcta acccataaac ccagcggcat catcgttgaa
     1741 tgccagcagg cccgcacgca gggagagaac agggaaaaag ccatggccat gctgaaaagc
     1801 cggctttatg aagaagagat ccggaaaaga gatgccatca agaatgccaa caacgccagc
     1861 aagaaaaaaa tagaatgggg atcccagatc cgctcctatg tttttcatcc ttataagatg
     1921 atcaaagatc accggaccga ttttgaaacc gggaatgttc agccggtcat ggacggggaa
     1981 ctggacggtt ttatcaaagc ttacctgatg acggaaaaag aaacggcaaa ctaaacttca
     2041 taaccattca aagagatcat catgaatctt ggttattttc attatgcgat gccttccaat
     2101 gaaccggtgc tgaactatgc acccggatct cctgaaaaaa tttccctgaa aaaaacaatc
     2161 cgggaaataa aaggacagga gcacgatatt ccgatgatca tcggcgggaa ggaagttcgt
     2221 tcgggaaata aaatcaaaat gcatcccccc catgaaattg cccataatct gggaagcttt
     2281 caccgggccg ggcctgatca tattcagcag gccattcagg cggccctcgc cgcgaaacca
     2341 gcctgggaag cgcttaactg ggaaaaccgg gcggctattt ttctgaaagc cgccgacttg
     2401 cttgcgggca aataccggtc ttacatgaat gcagcgacca tgatcggcca gagcaaaaat
     2461 ccttatcagg ctgaaattga cggggtatgt gaactgattg attttcttcg gtttaacact
     2521 cattatctca gtgaaatata caggcagcag cccaatagtt ccccgggtgt tcataaccgg
     2581 ttggaatacc gcccgctgga gggatttgtg ctggccatca ccccttttaa ttttacggcg
     2641 attggaggaa acctgcccag ctcggctgct ttatgtggaa acacggtggt ctggaaaccg
     2701 gcagacaccc aga
//
LOCUS       DASYCU010000424         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1400533, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000424 DASYCU010000000
VERSION     DASYCU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400533"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGM24_08565"
     CDS             complement(<1..597)
                     /locus_tag="VGM24_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY4155468.1"
                     /translation="MKKLLFLLYAPLLFFCACKKKDQGTALKGVWARKADFPGVKRQA
                     AVGFSIGGTGYIGTGYDGAIGLSDLYAYDPVSDSWTQRASLPVSPSLSRYGSVAFVID
                     GKAYLVGGNSATGFLKEVWEYDPANDMWTNKNPFPGPARGNGIGLSVGSFGYFGLGYS
                     IAPLAQLTDWWKYDPSTDQWTQMLDFPGPNIQSGMGFGL"
     gene            870..2393
                     /locus_tag="VGM24_08570"
     CDS             870..2393
                     /locus_tag="VGM24_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155469.1"
                     /translation="MKKLEKPEDKKQISRADFIKKSAMTAAGFYIVPRHVLGGPGFVA
                     PSDKLYIAAVGCGGEAASDIHHYATAPKKNAEIAFLCDVDDRMAAPRRKQFPKAGFYQ
                     DWREMFHKESKNFDAVSVAIPDHNHAIVGLGAMQLKKHLYLQKPLTHDIYEARILTQA
                     AEKYKVVTQMGDQGASCDGMRTLREWFEAGLIGDIEKVYNWTNRPVWPQGIPWPDKHP
                     PVPKELNWDLWLGTAEKTDYIDNLVPFNWRGWWRFGTGALGDMACHIIGPSFKLLQLG
                     YPTEVTCSASTIYSGIFKEAEFPESIPVSSKLRYTFKLKDGRDLKLYWMDGGMVPDRP
                     DELDPDVNENEALADIPQENDFEGGTLFVGTKGKVSCGWGGSHPRLLPLSRNKDINIP
                     EKYPRIEGGMDGHWWQWIDACIAGYGNAEVDSPFVGYAGPLTETVLMGNLLLRSFNLR
                     EKVKRNDPVYGSMEEFENPGRYISYKWDGENMRITNFEAANQFVKRKYREGWGELTL"
     gene            complement(2404..>3051)
                     /locus_tag="VGM24_08575"
     CDS             complement(2404..>3051)
                     /locus_tag="VGM24_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155470.1"
                     /translation="RNERGSYALLFKKAFIPVVSIGVALAMFQQLCGINVVFNYTSNI
                     FESIGASKDDQLLQTVFIGSVNLVFTVFAILLVDRLGRRPLMLIGAGGLGILYIIIAY
                     FLAIKSAMVSLFLLMAIGLFAATLAPVTWVLISEIFPSRIRVAASSFAILCLWFAFFI
                     TIFTFPMLQKAIGTNNTFYIYSVVCFLGFAFVWFRVKETNGKSLEEMDALFPAHG"
BASE COUNT          857 a          748 c          761 g          685 t
ORIGIN      
        1 cagtccaaaa cccattcccg attgaatgtt tggtcccgga aaatccagca tctgtgtcca
       61 ttgatctgtt gaaggatcat atttccacca gtctgtcagc tgggcgagcg gagcaatgct
      121 gtacccaagc ccgaaatacc cgaaactccc gacggataaa ccaattccat tcccccttgc
      181 aggacccgga aaaggatttt tatttgtcca catatcattt gccggatcat attcccagac
      241 ttccttcaga aacccggtgg ccgaatttcc gccaaccagg taagctttgc cgtctattac
      301 aaaagccaca cttccatacc gggaaaggga aggactcacc ggcaggctgg cgcgttgcgt
      361 ccagctgtcg ctgacgggat cgtaagcata gagatcgctc agtccgatcg ccccgtcata
      421 acccgtacca atgtagcctg ttccaccgat ggaaaatcct acggctgcct gccgcttaac
      481 ccccggaaaa tctgccttac gggcccacac ccctttcagc gcagtgccct gatctttctt
      541 tttacaggcg cagaaaaaca gcaacggggc ataaagaaga aataatagtt ttttcatgcc
      601 cggttttatt ataaaagtga atgttatggc gccggtactg cggtacaggc catgcgatca
      661 gacataaaat ttacggaaat tgttcaaccc ggccaagatt ctgaagaata tttccacaac
      721 ggatgctaaa taaaaaaccg gtccttcttt actgttccaa tgtctcttgt tcgaaccggt
      781 attttcaatt ttaatttccc gttcgcgatt tatggcgtaa tttaaaaggt attatcaggt
      841 gcattctttt accggacaaa tgtttctata tgaaaaaact cgaaaaacca gaagataaaa
      901 aacagatcag ccgcgcagac ttcatcaaaa aatcagcaat gaccgctgcc ggattttata
      961 tagtgccccg gcatgtgctg ggtggtcccg gctttgttgc tccaagcgat aaattataca
     1021 ttgccgccgt aggttgcggg ggagaagcgg ccagcgatat tcatcattat gccacggcgc
     1081 ccaaaaagaa tgccgagatc gcctttctct gtgatgtgga tgaccgtatg gcggcgcccc
     1141 ggcgcaagca atttccaaaa gccggttttt atcaggattg gcgggagatg ttccataaag
     1201 agagcaagaa tttcgacgcg gtttccgttg cgattccgga tcataaccac gccatcgtag
     1261 gtctcggcgc catgcaattg aagaaacacc tgtatttgca aaaaccgctc acgcatgata
     1321 tttatgaagc ccgcatcctc acgcaggcgg cggaaaaata taaggtagtc actcaaatgg
     1381 gcgatcaggg cgcctcctgc gacgggatgc gaacattgag ggaatggttc gaagcgggtc
     1441 tgatcggcga tattgagaag gtgtacaact ggacaaaccg ccccgtctgg ccgcagggga
     1501 taccctggcc cgataaacat ccacccgttc ccaaggagct gaactgggat ctctggctgg
     1561 gtacggcgga aaaaacagat tatatcgaca acctggttcc gttcaactgg cgtggctggt
     1621 ggaggtttgg caccggcgca ctgggagaca tggcctgcca tattatcggc ccttcattca
     1681 aattactgca actcggttat cccacggaag ttacctgcag tgcaagcact atctattccg
     1741 gcatcttcaa agaagctgaa tttccggaaa gcattccggt ttccagcaag ctgcggtata
     1801 ccttcaaatt gaaagacgga cgggatctga aactctactg gatggatggc ggcatggtcc
     1861 ccgaccgtcc ggatgaactg gaccccgatg taaatgaaaa tgaagcgctg gcggacatac
     1921 cgcaggaaaa cgattttgaa ggaggtacgc tgtttgtcgg aaccaaggga aaagtgtcct
     1981 gcggatgggg cggaagtcat ccccggctgc tcccactctc ccggaacaag gacatcaata
     2041 tccctgaaaa atatccgagg atcgaagggg gaatggacgg ccactggtgg caatggatag
     2101 atgcctgcat cgccggatat ggaaacgcgg aagtggattc tccatttgtc ggctatgccg
     2161 gtccgttgac cgaaacggtc ctgatgggaa atttgttatt gagaagcttt aacctgcgcg
     2221 agaaagtaaa acggaacgat ccggtttacg gcagcatgga agaatttgaa aaccccggcc
     2281 ggtatatcag ttacaaatgg gatggagaga atatgcggat aaccaatttt gaagcggcca
     2341 accaattcgt aaaaaggaaa taccgcgaag gctggggaga attgacactc tgagctccgg
     2401 aaattatccg tgggcaggga aaagggcatc catttcttcc agggatttgc cgttggtttc
     2461 tttaacacgg aaccagacaa atgcaaatcc caaaaaacag accaccgaat aaatgtaaaa
     2521 cgtgttattt gtgccgattg ccttttgcag catcgggaaa gtgaatatgg ttataaaaaa
     2581 ggcgaaccac aagcacaaaa tggcaaagga agaagcagcc accctgatcc tcgaaggaaa
     2641 tatttccgaa atcaggaccc aggtgacggg agccagcgtg gcggcgaata atccaatagc
     2701 catcagcagg aagagagaaa ccatagccga ttttatagcg agaaagtaag cgatgatgat
     2761 ataaagtatg cccagtccgc cggcgcctat cagcatcagg ggtctgcggc ccaggcggtc
     2821 caccagcaga atggcaaaaa ccgtaaaaac caggtttacg cttccgatga atacggtctg
     2881 cagcagctga tcatccttgg atgccccgat actctcaaaa atattggaag tgtagttgaa
     2941 taccacgttt attccgcaaa gctgctggaa catggccaga gcaacgccga tgcttactac
     3001 cggaataaag gcttttttaa aaagcagggc ataacttcct ctttcattcc g
//
LOCUS       DASYCU010000425         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5017763, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000425 DASYCU010000000
VERSION     DASYCU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5017763"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VGM24_08580"
     CDS             <1..480
                     /locus_tag="VGM24_08580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY4155471.1"
                     /translation="LGGYPGNDDQGREMQSKLDKNDILEDFIAAYGYLKDLKDCNGKV
                     GVVGFCFGGWIANMMAVRISGLSAAVPFYGTQPANEDVPKINAPLLLHYAGLDTRVNE
                     GWPAYEQALKDQKKEYTAYVYLDVNHGFHNDTTPRYDKPAAELAWKRTIDFFNEKLK"
     gene            542..1003
                     /locus_tag="VGM24_08585"
     CDS             542..1003
                     /locus_tag="VGM24_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4155472.1"
                     /translation="MKNSKKAAGKKAAGKKADPVVHFEFPADDSKRMAKFYTSAFGWK
                     TQMLGGDMGNYTLATTASDLDKTGRPKKAGIINGGFYTRSNDKPAQYPSVVIAVDSIK
                     KSMNRVTKAGGKVIGEPVEIPGFGMYVSFYDTEGNRVSIMEPTREMKRKTK"
     gene            complement(1085..2242)
                     /locus_tag="VGM24_08590"
     CDS             complement(1085..2242)
                     /locus_tag="VGM24_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEY4155473.1"
                     /translation="MKKNFTAFFGIFSVLAIIVFAVSCKKDGPQFCNCSPGDIVATQT
                     VFATGLNNPRGLKFGPDGRLYVAEGGVGGTNSTMGICDQVPGAGPYLGSDTGARISAI
                     DRWGGRTTVADKIPSSVTAPAVGSFVSGVADVAFIGNTLYGLLTSAGCSHGVPDYPNG
                     VFRVNKNKTWTLINNLSNFYQNHPAKNPNPNDFEVDGTPYSMISLDNRLFSIEPNHGE
                     LDEILLNGQIRKVIDISAAEGHIVPSALTYHNGNFYMGNLSTFPATPGISAVYKITPQ
                     GHISTYATGFNMVLGVQFDPLGGLYVLESTTGNPFPTPGTGDVIRIDPDGSRMTIASN
                     LNLPTAMTFGPDDKLYISDWGFGGAPGMGRIVQVDLTCARSHNFKKNGNAD"
BASE COUNT          629 a          560 c          602 g          514 t
ORIGIN      
        1 ctgggcggtt atcccgggaa tgacgaccag ggacgggaaa tgcagagcaa gctcgataaa
       61 aatgatattc tggaagattt tattgctgcc tacggatacc tgaaagattt aaaagactgc
      121 aatggaaaag ttggcgtcgt cggcttttgc ttcgggggct ggatagcgaa tatgatggcc
      181 gtccgcattt ccggcttatc ggcggcagtt ccgttttacg gcacccagcc ggccaatgaa
      241 gatgttccaa agatcaatgc tccgctcctg ctgcattatg cgggcctcga tacgcgggtg
      301 aatgaagggt ggccggccta tgaacaggcc ctgaaggacc agaagaaaga gtataccgcg
      361 tatgtttatc tggacgtaaa ccacgggttt cacaatgata ccacgccccg ctacgataaa
      421 ccggcggcag aacttgcctg gaagcgtacg attgattttt tcaacgaaaa attgaagtga
      481 ccgggatccg gatgaatgtt gctgagaccg ggaatcgcat taattaataa aaaacactaa
      541 aatgaaaaat tcaaaaaaag ccgccggcaa aaaggctgcc ggcaaaaaag ctgaccccgt
      601 cgtccacttt gaatttcctg cggatgacag taaaagaatg gcaaaattct ataccagtgc
      661 ctttggatgg aaaacacaaa tgctgggcgg ggacatggga aactatacgc tggcaaccac
      721 cgcttctgat ctggacaaaa ccgggagacc gaaaaaagca ggcattatca acggcggatt
      781 ttataccagg agcaacgaca agcctgcgca atacccctca gtggtcattg ctgttgacag
      841 cattaaaaaa tccatgaaca gggtaaccaa agccggcggc aaagtaattg gggaaccggt
      901 ggaaataccg ggcttcggaa tgtatgtttc cttttatgat accgaaggca accgggtgag
      961 cataatggag cccaccaggg aaatgaaacg caaaacgaaa taaccccggg cagtgacaac
     1021 tgccacgggg ctatttttac aacaagagtt ctcaggatta gttcagtgtc tgtcgaaccc
     1081 gggactaatc ggcatttcca ttctttttaa agttatgtga ccgggcgcag gttaaatcga
     1141 cctgcacgat ccggcccatg ccgggagcgc cgccaaaacc ccagtctgaa atatacagtt
     1201 tatcatccgg cccgaaggtc atggccgtgg ggaggttcag attagaggcg atggtcatcc
     1261 gggatccgtc cggatctatt ctgataacat ccccggtgcc cggtgtagga aagggattgc
     1321 cggtcgtgct ttccagaaca tacagtccgc ccaggggatc gaactgaacg cccagcacca
     1381 tgttgaagcc ggtggcatag gtgcttatat ggccctgagg ggtgatctta tagacggctg
     1441 aaatgccggg tgtagccggg aaagtagaca aattgcccat ataaaaattg ccattatggt
     1501 aggtcagggc ggaaggaacg atatgacctt ccgctgcaga gatatcgata accttccgga
     1561 tctgaccgtt caataagatc tcgtccagct ccccgtgatt cggctcgata gaaaacaggc
     1621 gattatccag gctgatcatg ctatagggag taccgtcgac ctcgaaatca tttggatttg
     1681 gattcttggc cgggtggttc tgatagaaat tgctcaggtt gttgatcaaa gtccaggttt
     1741 tgtttttatt taccctgaac acgccgttcg ggtaatcggg tacgccatga ctacagccgg
     1801 cgctggtcag caggccatat agggtattcc cgataaaagc gacatccgcc accccgctga
     1861 caaaacttcc cacggcgggg gccgtaacgc tggacgggat tttgtccgcc accgttgtcc
     1921 ggccgcccca acgatctatc gcggagatcc ttgcaccggt atcactgccc aaatagggac
     1981 ctgctcccgg cacctgatcg cagatcccca tggttgaatt ggttcccccg acccctcctt
     2041 cagcaacata cagtcttccg tcaggtccga atttcaatcc gcgcggatta ttcaggcccg
     2101 tagcgaatac cgtctgggta gccacgatat cgccgggact gcagttacag aattgcgggc
     2161 cgtccttttt acaggaaacc gcaaagacta tgatcgccag tacagagaag attccaaaaa
     2221 atgcagtaaa gttttttttc atacaaattg cttgataagg tgataaataa aaatgactag
     2281 atttaaaggc aggaaaatcc ctttg
//
LOCUS       DASYCU010000426         4267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2410970, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000426 DASYCU010000000
VERSION     DASYCU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410970"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VGM24_08595"
     CDS             complement(<1..683)
                     /locus_tag="VGM24_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155474.1"
                     /translation="MSVAAALGGLLFGFDTAVISGAIPYIKNYFSLNEFMLGWAVSSI
                     LIGCGAGAVLAGPLADRYGRKWVLLCCAVLFALSGVGAALSGTLLQFVFFRLLGGLGV
                     GAAAMVSPMYIAEISPSFLRGRLVAMYQLAIVTGILLAYFSNYLLAGAGANNWRWMFA
                     SQTAPSLLFLLVLLRIPETPRWLIKKDRTEQARIILQKTEYGGDHEKEIAAIRQSFSN
                     DSAPRLSEM"
     gene            980..1849
                     /locus_tag="VGM24_08600"
     CDS             980..1849
                     /locus_tag="VGM24_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY4155475.1"
                     /translation="MKAHFEQVNADAGNSFMCKKIQSHHFDTPYHFHPEYELTRILKS
                     EGTRFIGSHFESFSAHDLLLIGKDVPHCWKNENNLRKDHVAEAIVIQFREDFIGKEFF
                     HKPELARIRSLLDRSQRGIAVKGRTKEQVAEMMEDMLEADPAQKLIDLLQILDLLSRS
                     EEYELLNLAGASAKMSPEDCERVNKIYSYIEENFHSEILLKDIAGMVYMTPSSFCRYF
                     KKISRKTLNTLINEYRIELSKQKLINTESSIYAICFDCGFQNISNFNKHFRRQTNLTP
                     SEYRAKFSAYLHD"
     gene            complement(1852..2763)
                     /locus_tag="VGM24_08605"
     CDS             complement(1852..2763)
                     /locus_tag="VGM24_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY4155476.1"
                     /translation="MAGEDLSKVHGLISKLFHWPSRKEDWEQYRLTDEQVAHFAEFGY
                     VSGIKLLDAQQTGELKNELQELSNPGHPGNSLFYEYSTNESTDPSKVLFHALGAWRIA
                     PGFHDLLWNPAFLMAASQLLGNQAVRFWHDQLFCKPARHGGVVAWHQDYSYWIRSVPM
                     QHLTCWCGLDDADTDNGCLYYVPGSHRWGLLEKPALAGDMDGLLLSLTGKQKAMFKPV
                     PIPLKAGYATFHHSLLIHGSYANTTERQRRAFVVNVFADGTRSNTNEPLLSGIPVFEK
                     DKKLEGDFFPLLFDPVREGIKLERHPA"
     gene            complement(2788..>4267)
                     /locus_tag="VGM24_08610"
     CDS             complement(2788..>4267)
                     /locus_tag="VGM24_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HEY4155477.1"
                     /translation="VGDGLSLNTAALQQAIDDCAKKGGGKVVVPAGNFVTGSLRLLSN
                     INFCLEAGAVLTGSPDRKDYLNQKDFGFSGSGAGKKTGILFASGAENVSLTGEGTING
                     QGGLFVYADSLQNGKDFDAKYTRQGNDYMNPAYGRADGPVMWKGSNDERPGTLVTFSN
                     CKNVHVTDIRFVAAPNWTMDFLNCENVKTRGISIDNNMDIPNSDGIDMYDSRQVTISD
                     CVIKAGDDAIAVVSSSDITVNNCVLHSRSSGIRIGYNVFNHHNSGNLLFNNIRIYESN
                     RGIGIFQRQEGDMQNMVFSNMIIDTRLHSGQWWGHGEPIHISAVPGLGSSKVGKISNV
                     RFSNINATAENGILFYGSKESVLENIELDNVNLTIRRGELSDSYGGNIDLRPTNDISL
                     GIFRRDLPAVLASNVKGLQIKDLRVQWGEHVPAYFTHALECTNFDGVSIEGLDQQGVS
                     APAGKQAAVYLHDGKNASVHRIVSGIPGTIPLLEQKVTNRIK"
BASE COUNT         1091 a         1114 c         1037 g         1025 t
ORIGIN      
        1 aacatttcag agaggcgggg agcgctgtcg ttgctgaaac tctgcctgat agccgcgatc
       61 tctttttcgt gatcaccgcc gtattccgtc ttctgcagga taatgcgggc ctgttccgtc
      121 ctgtctttct ttatcagcca ccggggcgtt tccgggatgc gcaacagcac gagcagaaac
      181 aaaagggacg gcgccgtttg ggaagcaaac atccatctcc agttattcgc gccggcgccg
      241 gcaagcaggt aattggaaaa ataagcgagc agtatcccgg ttacgatcgc aagctggtac
      301 atggcaacca gccttccgcg aaggaaagaa ggggatatct ccgcgatgta catcggggat
      361 accatcgcag ctgctcccac gccaagacca cccagcaggc ggaagaaaac aaattgcagc
      421 agcgtgccgg agagcgccgc gcctacgccc gacaaggcaa aaagaacagc gcagcataaa
      481 agaacccatt tcctcccgta gcggtctgcg aggggtccgg ccagcacagc gccggctccg
      541 cagccgatca ggatggaact tacggcccag cccagcatga attcgttcag cgaaaaataa
      601 tttttgatat acgggatcgc acccgaaatg acggctgtat caaaaccaaa taacagtccg
      661 ccgagagccg ccgccacgct cactttcaag ataaaaaaaa gatttggctt ttttttaact
      721 gccgattcag ggagagcagc cggggttgaa acgagagctg aatgcattgg gaagccgctg
      781 gataatcgtt aaaaggctaa aataaaggct tatgccggtt ttatctttta caaaattgcc
      841 tcatcactat acaatattac ccatcttgtc aggatccggg attttccggc tgacggatga
      901 tatatttaaa taattaattt tatggtttca acagcccgcc attaccaatc cgtcataagc
      961 tgtaaaattt ccgttaaaga tgaaagcaca ttttgagcag gtgaacgctg atgcagggaa
     1021 ttcctttatg tgcaaaaaga tccagtcgca tcattttgat accccctacc attttcatcc
     1081 ggagtacgaa ctgacccgga tcctaaaaag cgaaggcacg cgttttatcg gcagccactt
     1141 cgaatccttt tcggcacatg acctgttgct catcggcaag gacgttccgc attgctggaa
     1201 aaatgaaaat aacctgagaa aagatcatgt ggctgaagcc atcgttatcc agttcaggga
     1261 agattttatc gggaaagaat tttttcataa accggagctg gccaggataa gatcattgct
     1321 ggaccggtcg cagcgtggca tcgcggtaaa aggccgcaca aaagagcagg tggcggaaat
     1381 gatggaggac atgctggagg ccgatcccgc gcagaagctg attgacctgc tgcagatact
     1441 cgacctgctt tccagaagtg aagaatacga actcctgaac ctggcgggcg ccagtgctaa
     1501 aatgtctccg gaggactgtg aacgggtcaa taagatctat tcttacatcg aggaaaattt
     1561 tcattccgaa atcctgttaa aggatattgc cggcatggtg tatatgactc cttcctcctt
     1621 ctgccggtat tttaaaaaaa taagccggaa gaccttaaat accctgatca atgaataccg
     1681 cattgaactg tccaagcaaa aactgatcaa tacggaatcc agtatttatg cgatctgctt
     1741 tgattgcggc tttcagaaca tttccaactt caataagcac ttcagaagac agacaaacct
     1801 gacgcccagt gaataccggg ccaaattctc tgcctatctc catgactgag cttaagcggg
     1861 gtgccgttcc aatttaattc cttcccggac cgggtcaaaa agaaggggaa aaaaatctcc
     1921 ttccagcttt ttatctttct caaaaacggg aataccggat aaaagcggtt cattggtatt
     1981 ggaccgcgtg ccatcggcaa aaacattcac gacaaaagca cgccgctgcc gctccgttgt
     2041 attggcataa gagccgtgaa tcaggaggga atggtggaaa gtggcatacc ccgctttcaa
     2101 cggaatggga accggtttaa acatcgcttt ttgttttccc gtcaaagaaa gaaggagtcc
     2161 gtccatgtct cccgcgagtg cgggcttctc cagcaatccc cagcgatggc ttccgggaac
     2221 ataatacaaa catccattgt ccgtatcggc atcgtccagt ccgcaccagc aggtcaggtg
     2281 ctgcatcgga acgcttctga tccaatagga atagtcctgg tgccaggcga ccacgcctcc
     2341 gtgccgcgca ggcttgcaaa acaactggtc gtgccagaag cggacggcct ggttgcccaa
     2401 taactggctt gctgccatga gaaatgccgg attccacagg agatcgtgaa aacccggcgc
     2461 gatccgccag gcgcccaatg catgaaacag cactttgctg ggatccgttg attcgttggt
     2521 tgaatattcg taaaataaag aatttccggg atgcccgggg ttggataact cctgcaattc
     2581 atttttcaat tcgccggtct gctgcgcgtc gagcaatttg attccggata cgtaaccgaa
     2641 ttctgcaaaa tgcgccacct gctcatcggt aagccggtac tgttcccaat cctctttccg
     2701 agaaggccaa tgaaataatt tgcttataag gccatgcact ttactgagat cttctccagc
     2761 catggtatat tttttaatag taaagaatca tttaatcctg ttcgttacct tttgttccaa
     2821 cagaggaatt gttccgggta tccccgaaac aatacggtgc acgctggcgt ttttcccgtc
     2881 atgcaaatag acggcggcct gctttcctgc gggtgcggaa actccctgct gatccagccc
     2941 ttcgatactg accccgtcga agtttgtgca ttccaatgcg tgggtaaaat aggcggggac
     3001 gtgttcaccc cactgtacgc gcaggtcttt tatctgcagc cccttcacgt tggaggcaag
     3061 caccgccggc agatcccttc ggaaaatgcc cagggaaata tcatttgttg gacggaggtc
     3121 gatattgcca ccataactgt ctgacaattc cccgcgccgg atggtcaggt tcacattatc
     3181 cagctcgata ttttccagca cgctttcttt ggagccatag aataaaatcc cattctccgc
     3241 cgtagcatta atattggaga aacgcacatt gctgatcttg ccgaccttgc tgcttcccag
     3301 gcccggcacg gcagaaatat ggatcggttc gccatgcccc caccactggc ccgaatgcag
     3361 gcgcgtatcg atgatcatgt ttgaaaagac catattctgc atgtcccctt cctgacgctg
     3421 gaaaatcccg ataccgcggt tggattcata tatccggata ttattgaaca gcaggtttcc
     3481 cgaattatga tgattgaata cattgtagcc gatgcgaatg ccgctggagc gggaatgcaa
     3541 aacacaatta ttcacggtga tatcgctgga gctcacaacc gcaatggcat cgtcgcctgc
     3601 tttaatcacg caatcggaga tggtcacctg cctggaatcg tacatatcaa ttccatcgct
     3661 gttcggaatg tccatgttgt tgtcgatact tatcccgcgg gttttgacat tttcgcagtt
     3721 caggaaatcc attgtccagt tgggcgccgc gacgaagcgg atatcggtca cgtgcacgtt
     3781 tttgcaattg gaaaacgtga cgagggtacc gggccgttca tcattggatc ccttccacat
     3841 cacgggtccg tctgcacggc cgtacgcggg attcatatag tcgtttccct gccgcgtgta
     3901 ttttgcatca aaatcctttc cattctgcag gctgtctgcg tatacgaaaa gacccccctg
     3961 cccattgatg gtgccttcac ccgttaaaga aacattctcg gcgccgctgg caaagaggat
     4021 accggttttt ttgccggctc cggaaccgct gaacccaaag tccttttgat tgagatagtc
     4081 tttgcggtca gggcttcccg ttagtactgc tcccgcttcc aggcaaaaat tgatattgct
     4141 cagaagcctc agggaacccg tcacgaaatt gccggcggga acgaccacct taccaccgcc
     4201 ctttttggcg cagtcgtcaa tggcctgctg cagcgcagcg gtattcagcg aaaggccgtc
     4261 accgacg
//
LOCUS       DASYCU010000427         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_965194, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000427 DASYCU010000000
VERSION     DASYCU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965194"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VGM24_08615"
     CDS             <1..299
                     /locus_tag="VGM24_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155478.1"
                     /translation="RGVMEKCSFCVQRLQDGKLKAKKEDRPLLDMKDVRTSCQQACPT
                     TAIVFGNINDSKSAVVKERVDNGHRLYHVLEELHVLPNISYLAKVRNPEAEANS"
     gene            361..1809
                     /gene="nrfD"
                     /locus_tag="VGM24_08620"
     CDS             361..1809
                     /gene="nrfD"
                     /locus_tag="VGM24_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY4155479.1"
                     /translation="MALLKYESQVRPPLIEGDKSYHQITEDIVRPIEARPSKLWWFGF
                     IFAVLLLCFGIFSVTREVIYGIGQWNLHKTVGWGWDITNFVWWVGIGHAGTLISAILL
                     LFRQGWRTGVNRAAEAMTIFAVMCAGQFPIWHMGRVWMAFFVLPYPNTRGSLWPNFNS
                     PLLWDVFAVGTYFTVSVLFWYCGLLPDLATVRDRAKLKWRKFFYGLTSFGWSGSTKHW
                     QRHEYLSLILAGLATPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAVFSGFAMV
                     QTLLIITRRVFRLQEYITLEHIDVMNKIIVTTGSIVGIAYLTELFVAWYSQNPYEQSQ
                     FMQSRANLFTPYGWAYYIMMSCNVLSPQIFWSRKMRRNLTVTFFMSIIVNIGMWFERF
                     VIIVSSIYRDYLPSSWATYYSPSIWEVGFYLGTFGLFFTCYFLFSKFFPVIALAEIKH
                     ILKKSGNNYKEQMGEAETISPEAFAHEMAHGH"
     gene            1857..2447
                     /locus_tag="VGM24_08625"
     CDS             1857..2447
                     /locus_tag="VGM24_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEY4155480.1"
                     /translation="MAIKKFVVGNFEDEAVLFKAVKKVRLAGYKLHDVFTPMPIHGLD
                     KAMGLRETSIPTAGFVYGITGTTTALCGISYVLAYDWPLNIGGKPHFALPAWIPIMFE
                     LTVLFSAVGMVLTFCYLCQMAPFVRKHHFSLRSTDDRFVMVVECTEKTNEEELKSFLQ
                     QAGADEINTQMAETGWWLGSYSKNKKPFENPNTILA"
     gene            2464..3138
                     /locus_tag="VGM24_08630"
     CDS             2464..3138
                     /locus_tag="VGM24_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY4155481.1"
                     /translation="MRITKFIIPFCLALFLLAACSDEVRRSPGRVYMPDMAYSRAYET
                     YSVTPEEKEALLKKGIHYSNIPVAGTIKRGELFPFPLAKDKEGDSTNYVASKAIKNPI
                     PTLDSESMVEAHRLYLVNCGICHGPKLDGNGPLYRGGDGPFPAKPATLVGDAKYEAMP
                     EGQMYYSVTYGKNKMGSYASQLSTRQRWMVIDFVKSMQAGAKASHAGAAGADSTAKAA
                     PSAAKK"
     gene            3171..4394
                     /locus_tag="VGM24_08635"
     CDS             3171..4394
                     /locus_tag="VGM24_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HEY4155482.1"
                     /translation="MSLNERFEITPGFRMWSLVLMGVGVLSLIIGYIVYGTGDETHQA
                     IFWGSLLHNAIYFLLITNAVMFFFCATTLSLAGFQISFKRVTEAISACVPVIGGITFV
                     IMLFIVFGHKSMVYQWLDKAAVAKDAALTGKKGFLNPAFFLTWTIVAIGLWSILGYKM
                     RSLSRKTDDHPLSVEEGKKFMFKNTVWASLFIIWYALTIAAIIPWLWLMSLNAHWFST
                     LYSWYVFASTFVAGIALISVFVVYMKNRGYLQYTNKEHLHDLGKFQFAFSIFWTYLWF
                     AQFMLIWYANISEETVYFRARAEGAYSGIFYLNLVINFLAPILIFMSTPTKRNYTVVA
                     FMSVALLFGHWLDFYQIIFAGMVPDHVPMGLYDFGIAAGFVGVIIYQTGRVMAKFPLL
                     ARNHPFFKESIIHYT"
     gene            4445..>4797
                     /locus_tag="VGM24_08640"
     CDS             4445..>4797
                     /locus_tag="VGM24_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY4155483.1"
                     /translation="MSTFFLIAILLLGFVITFQIAKASEYVGILKGEKKNFQQNNRIN
                     GFLMIVFLVLGLIGVYWCNELFKGRILGEAASDHGKKIDTMLYITIAITGVVFFITQI
                     LLFWFAFKYQHSEKR"
BASE COUNT         1116 a         1137 c         1273 g         1271 t
ORIGIN      
        1 gccggggggt tatggagaaa tgttccttct gcgtgcagcg cctgcaggat ggaaagctga
       61 aggcaaagaa ggaagacagg cccctgctcg atatgaagga cgtgagaaca tcctgccagc
      121 aggcttgtcc gaccaccgcc attgtttttg gaaacatcaa cgacagcaag agcgctgtgg
      181 tgaaagagcg cgtggataac ggccatcgcc tctatcatgt actcgaagaa ttgcacgtat
      241 taccgaatat cagttacctc gccaaagtcc gtaacccgga ggccgaggcg aattcataaa
      301 gaggggagat aataaagaat gccggaagat ccggccggat ttaaaacgta ttaaccgact
      361 atggcactac taaaatatga gtcacaggtg aggccaccgc tgatcgaggg cgataaatcc
      421 tatcaccaga tcaccgaaga tatcgtgcgg cccattgaag cgagaccatc aaagctctgg
      481 tggttcgggt ttatatttgc cgttttgctt ttgtgtttcg gaattttttc tgtcacccgg
      541 gaagtgatct acgggatcgg ccagtggaac ctgcacaaaa cagtgggttg gggttgggat
      601 atcaccaatt tcgtatggtg ggtggggatc ggtcatgccg gaaccctgat ctccgcgatc
      661 ttactcctgt tccggcaggg ctggaggacc ggcgtgaacc gggctgcgga agcgatgacc
      721 atttttgcgg tgatgtgcgc cgggcaattc ccgatctggc atatgggccg cgtctggatg
      781 gccttcttcg tactgcctta ccccaatacg cgcggatcgc tctggccgaa ttttaattct
      841 cccctgctct gggatgtatt tgcggtaggc acttatttta ccgtttccgt gctgttctgg
      901 tattgcggac tgctgcctga cctggccacc gtccgggacc gggccaaact gaaatggaga
      961 aaattctttt acgggctcac ttctttcgga tggagcggat ccaccaaaca ctggcagcgg
     1021 catgaatacc tgtcgctgat cctcgccggg ctggccacgc cgctggtgct ctccgtacat
     1081 accatcgtgt ctttcgactt tgccacttcg gtgattcccg gatggcatac caccatcttc
     1141 ccgccctatt ttgtggccgg ggccgtattc tccggatttg ccatggtgca aaccttattg
     1201 atcattaccc gccgggtatt caggctgcag gaatatatca cactcgagca tatcgacgtg
     1261 atgaacaaga tcatcgtcac caccggttcc attgtcggca ttgcctatct caccgaactg
     1321 tttgtagcct ggtacagcca gaatccctat gagcaatcgc agttcatgca gagccgggcg
     1381 aatctcttca ctccttatgg ttgggcatat tatattatga tgagctgtaa cgtgctttct
     1441 ccccagatct tctggtcgcg caaaatgcgg agaaacctca ccgttacttt tttcatgagc
     1501 atcatcgtga atatcgggat gtggttcgag cggttcgtga tcatcgtttc ttccatttac
     1561 cgggattatc tgcccagttc ctgggccact tactattcgc cgagtatctg ggaggtcgga
     1621 ttttatttgg ggaccttcgg gttattcttt acctgttact ttttgttctc gaaattcttc
     1681 ccggtgatcg cgctggcgga aataaaacat attcttaaaa aatcgggcaa taattataaa
     1741 gagcagatgg gcgaagcgga aacgatctcg ccggaggcat tcgcccatga gatggcgcac
     1801 ggacattagg gagcgatggt ttcggctatt attttttgat ttttgatttt acgattatgg
     1861 caattaaaaa atttgttgtc ggcaattttg aggacgaagc ggtcttattc aaggcggtga
     1921 agaaagtgcg tctggccgga tataagctgc acgatgtttt caccccgatg ccgatccatg
     1981 gtttggataa ggccatgggc ctgcgggaga cgagcattcc gacagccggc tttgtctacg
     2041 gcatcaccgg aaccaccacg gctttgtgcg ggatctctta tgtgctggca tacgactggc
     2101 cgctgaatat cggcggtaag ccgcattttg cgctgccggc ctggatcccg atcatgtttg
     2161 agctgaccgt acttttttct gcggtaggca tggtgcttac cttctgctac ctctgccaga
     2221 tggctccatt tgtgcggaaa catcatttca gcctccgttc cacggacgac cggtttgtga
     2281 tggtggtgga atgcacggaa aagacgaacg aggaagagtt gaagagtttt ctgcagcagg
     2341 cgggagcgga cgaaatcaac acgcagatgg cggaaaccgg ctggtggctg ggatcctaca
     2401 gtaagaataa aaagccattc gagaacccta acactattct ggcataacga aatgaacctt
     2461 gcaatgcgga tcacaaaatt tataattcct ttctgtttag ccctctttct gctcgctgcc
     2521 tgcagtgatg aagtgaggcg gagtccgggc agggtgtaca tgccggacat ggcgtacagc
     2581 cgggcctatg aaacgtattc cgtaacaccg gaagaaaagg aagccctgct gaagaaggga
     2641 attcattaca gcaatatccc ggtagcggga accatcaagc ggggagaact atttcctttc
     2701 ccgctggcga aggataaaga aggagactcc accaattatg tggcctccaa agcaataaag
     2761 aatcccattc cgaccttgga ttcggagagt atggtggaag cgcaccgctt gtacctggtg
     2821 aactgcggaa tctgtcatgg tccgaagctg gacggaaacg gtccgctcta ccggggcggt
     2881 gacggtcctt tcccggcaaa gcccgccacg ctggtggggg atgcgaaata cgaagcgatg
     2941 ccggaaggcc agatgtatta ttccgttacc tatggtaaga acaagatggg cagttatgct
     3001 tcgcaactga gcacccgtca acgctggatg gtgattgatt ttgtgaaaag catgcaggct
     3061 ggtgcaaagg catcgcatgc cggcgcggca ggagccgatt ccacggcaaa ggcggcgcct
     3121 tccgcagcaa agaaataaag taactgagaa ccgaataaaa ttttttacga atgtcactga
     3181 atgaacgttt tgaaataact cccggtttca ggatgtggtc gctggtcctg atgggcgttg
     3241 gagtgctgag cctgatcatc ggatatattg tttacggcac cggcgacgaa acgcatcagg
     3301 cgatattctg gggctccctg ctgcacaacg ccatttattt cctgctgata acgaatgcgg
     3361 tgatgttttt tttctgtgcc accaccctgt cgctcgccgg ttttcaaatc tctttcaaac
     3421 gggtaacgga agccatctcc gcctgtgttc cggtgatcgg cggcatcacg ttcgtgatta
     3481 tgttgtttat tgtatttggc cataaaagca tggtctacca atggctggac aaagcggccg
     3541 ttgccaagga tgctgcgctc accgggaaaa aggggtttct gaatccggca ttctttctca
     3601 cctggacgat cgtggccatc ggattatgga gcatcctcgg atataaaatg cggagcctga
     3661 gccggaagac cgacgaccat ccgctgagtg tggaagaagg aaagaagttc atgttcaaga
     3721 atacggtctg ggcctccctc ttcattatct ggtatgcgct aacgattgcc gccattattc
     3781 cctggctctg gctgatgagc cttaacgcgc actggttctc cacgctgtac agctggtatg
     3841 tatttgcaag cacttttgtg gcgggcattg cgctgatctc ggtattcgtg gtctatatga
     3901 agaaccgcgg atacctgcag tataccaata aggaacacct gcacgatctc ggaaagttcc
     3961 agtttgcctt ttctattttc tggacctatc tctggtttgc gcagttcatg ctgatctggt
     4021 atgcgaatat ttccgaggaa acggtttatt tcagggcgcg cgcagaaggc gcctattccg
     4081 gaatatttta tttaaacctg gtaatcaact tcttggcgcc catattgatt ttcatgagca
     4141 cgcccaccaa acgcaattat accgtcgtag cttttatgtc tgtagctttg ctctttggac
     4201 actggctgga tttttaccag atcatttttg cgggaatggt accggatcat gtgcctatgg
     4261 gcctttatga tttcggcatt gccgccggat ttgtgggggt gatcatctac cagaccggcc
     4321 gggtgatggc gaagttcccg ctgctggcca ggaaccatcc gttctttaaa gagagtatta
     4381 ttcattatac ttagtcgttg tttacggaga ggagatttaa aacgattaaa tttttttaag
     4441 tatcatgtca actttttttc tcattgcgat tttattactg ggttttgtga tcacatttca
     4501 gattgccaaa gccagcgaat atgtgggcat cctgaaaggt gaaaagaaaa atttccagca
     4561 gaataaccgg atcaacggat tcctgatgat cgtttttctt gtactcggcc tgatcggcgt
     4621 gtactggtgt aatgaactgt tcaagggaag gatcctgggg gaagccgctt cggatcatgg
     4681 gaagaagatt gacacgatgc tgtatatcac cattgccatc accggcgtgg ttttcttcat
     4741 tacccagatc cttttattct ggtttgcatt taaataccag cattctgaaa aaagaaa
//
LOCUS       DASYCU010000428         4956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1543113, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000428 DASYCU010000000
VERSION     DASYCU010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543113"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VGM24_08645"
     CDS             complement(<1..510)
                     /locus_tag="VGM24_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G-D-S-L family lipolytic protein"
                     /protein_id="HEY4155484.1"
                     /translation="MKMLRTFLLLLTTLAVGNCLAQPFSDEIREFKKQDSIQSPPANA
                     ILFVGSSSFRKWQDVGKYFPGYTIINRGFGGSTLPDVIRYANEIIIPYHPKQVVIYCG
                     DNDLAASDKVTADSVFERFKTLFEIIRSGLPGVSVLFVAIKPSPSRERLWPKMKQANL
                     LIKTFLSLQT"
     gene            complement(526..2274)
                     /locus_tag="VGM24_08650"
     CDS             complement(526..2274)
                     /locus_tag="VGM24_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY4155485.1"
                     /translation="MKIRLVFLPGLICLFLHSRAQQQTYCNPINIDYGYTPIPDFSEA
                     GRHRATADPTITLYKGDYYLFSTNQWGYWWSHDLLNWHFIKRSFLKPWHHVYDDLCAP
                     ATLVLGDSLLVIGSTYTKNFPLWMSTNPKQDQWKEAVDSFQAGAWDPALFLDDDGRIY
                     LYHGSSNFYPIYGWEIDRKTLQPIGEHLELIKLHDDLHGWERFGEYNDNNFLSPFIEG
                     AWMNKHNGKYYLQYGAPGTEMSGYGDGVYVGDHPLGPFTYQAHNPFSYKPGGFSRGAG
                     HGATFQDRDGGWWHVSTMVVNMKNTFERRIGLWPAGFDGDGILYSNTAFGDYPLYLPA
                     AHKDHLSGSFTGWMLLNYNKPVMVSSTLGGYAANNAVDENIRTYWSAATGNPEEWIQT
                     DLGAFSTVRAIQINYADQDVDSSFLGKISGLYCQYKLWESADGKNWKILVDKSRNHTD
                     IPQEYVELPKPVRTRYIKLENIHMPTGKFAVSGLRVFGKGEGRIPDTVSHFIVLRSEN
                     DSRNAFIKWFTVTGATGYNIYRGIAPDKLYNNVMVYNDNQYYFKPMDKGRTYYFSIEA
                     FNENGISPRTKPVEVK"
     gene            complement(2302..3669)
                     /locus_tag="VGM24_08655"
     CDS             complement(2302..3669)
                     /locus_tag="VGM24_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY4155486.1"
                     /translation="MKYLSIKAGWLTALFFSLVFVSFGQPVKPVSKNPAAPEERKVLS
                     DSALLDLVQKQTFSYFWDFAHPLSGLARERSNNAYGYGDEVVTTGGSGFGIMAIIVAA
                     DRHWISRDTAVKHLLKTVSFLMKANSYHGIFPHWMNGTTGVTIPFSRKDDGADVVETS
                     YLFEGLLCARQYFNRNERLENELRNRINSLWREAEWSWHQRDGGNSFYWHWSPNNGWS
                     MNFELRGWNECLITYVLAASSPTYPITADVYRRCWAESNHFLNGRSYYGITLPLGFDF
                     GGPLFFAHYSFMGLDPHGLKDEYADYWIQNYHHTLINYKHCVINPNHFKGYGENCWGL
                     TASDTYNGYNAHSPTNDLGTITPSAALSSFPYTPEYSMKALKHFYYDLGDKIWGKYGF
                     TDAFNETKNWYARNDLAIDEGPIVCMIENYRSGLLWKLFMSCPEIESGLKKLGFESPY
                     FQARQ"
     gene            complement(3712..>4956)
                     /locus_tag="VGM24_08660"
     CDS             complement(3712..>4956)
                     /locus_tag="VGM24_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY4155487.1"
                     /translation="TNKFPPVTDSALLTLVEQQTFKYFYDFGHPVSGLARERNSSGDV
                     VTTGGSGFGIMSLLVGIQRNFISRASGLARLRLIVDFLSTKAQRYHGAFPHWMNGATG
                     ETVPFSPQDDGADLVETSYMLQGLLCARQYFNNTSDPDETALRDSINSIWQGVDWQWF
                     RQNGQDVLYWHWSPDMGWAINMKISGWDEALIVYALAASAPAGAISKTVYDNGWAQNG
                     TMRNGNSYYGITLPLGPPLGGPLFFAHYSFLGINPAGLSDAYANYWTQNTAHAQINYK
                     YCVANPMGYYGYSNLCWGLTASDDNISGYSAHSPTNDLGIISPTAAISSLPYTPTESM
                     NALKFFYYTLGDKLWGEYGFVDAFSLSDPWFADSYLAIDQGPEIVMIENYRSGLLWNL
                     FMSCPEIKTGMKALGFQSPRLN"
BASE COUNT         1183 a         1239 c         1298 g         1236 t
ORIGIN      
        1 cgtctgcagg gataaaaatg ttttgatgag caggttggcc tgcttcattt tgggccagag
       61 ccgttcccgg ctggggctgg gttttatcgc aacgaaaaga acactcaccc cgggcagtcc
      121 cgaccggatg atctcaaaca aggttttgaa tcgttcaaaa acgctgtccg ccgttacttt
      181 atcggaggcc gccaggtcat tatccccgca atagatcacc acctgtttcg gatgataggg
      241 aataatgatc tcgttcgcat accggatcac atcgggcagg gtggaacctc cgaaaccgcg
      301 attgataatg gtatatcccg gaaagtattt acccacgtcc tgccatttcc ggaatgagga
      361 actgccgaca aacagaatgg cattggccgg gggggactga atgctgtcct gctttttaaa
      421 ttcccggatc tcatcggaaa agggttgtgc caggcagttg ccaacggcca gcgtggtcag
      481 taacagcaga aaggttctca gcattttcat gatcgtgaat ttaatttatt ttacttcaac
      541 aggttttgtt ctgggagaaa ttccgttttc gttaaaggct tcaatggaaa aataatacgt
      601 cctgccctta tccatgggtt taaaataata ctggttgtcg ttatagacca tgacattatt
      661 atataattta tccggagcga tgcccctgta aatattataa cccgtagccc cggtgaccgt
      721 aaaccatttg atgaatgcat tgcgggaatc attttcactg cgcagcacga tgaaatggga
      781 aacagtatcc ggtatccggc cttcgccctt tccaaatacc cttaacccgc tgacggcaaa
      841 tttaccggtt ggcatgtgga tattctcgag ttttatataa cgggtgcgga ccggtttggg
      901 caattctaca tattcctgcg gaatatccgt atggttgcgg cttttatcca ccaggatctt
      961 ccagttcttc ccgtccgcgg attcccagag cttatactgg cagtaaagac cgctgatctt
     1021 cccgagaaag ctgctgtcca catcctgatc ggcataattg atctgaatgg cacgaaccgt
     1081 actgaatgca cccaggtcgg tttgaatcca ttcctcgggg ttgcccgttg ccgcactcca
     1141 gtaggtgcgg atattttcat ccacggcgtt gttggcggca tatccgccca gcgtggaaga
     1201 gaccatcacc ggtttattgt agttgagcag catccatccc gtgaagcttc cgctgagatg
     1261 gtctttatgc gcagcaggca gataaagggg ataatcgccg aacgcggtat tggaatataa
     1321 tattccatcc ccgtcaaaac cggcgggcca cagaccgatc cttctttcaa acgtattttt
     1381 catattcacc accatggtgg acacatgcca ccatccgcca tcgcgatcct ggaaggtggc
     1441 gccatgcccg gctccgcgcg aaaagccgcc gggtttataa gaaaagggat tatgcgcctg
     1501 gtaggtaaag ggaccgagcg gatgatcgcc gacatagacc ccgtcgccgt aaccgctcat
     1561 ctcggtgccg ggcgcgccgt actgcaggta gtacttaccg ttatgcttat tcatccaggc
     1621 gccttctata aaggggctga ggaaattatt atcgttgtac tccccaaacc gttcccatcc
     1681 gtgcagatcg tcgtgcaatt tgatcagttc caagtgttca ccgattggct gcagggtctt
     1741 cctgtcaatt tcccagccgt atatgggata gaaattgctc gacccgtggt ataaataaat
     1801 ccttccgtca tcgtccagga acaaagcggg atcccaggct cctgcctgaa aggaatccac
     1861 ggcttccttc cactggtcct gtttcggatt cgtgctcatc cagagcggaa agtttttggt
     1921 ataggtggaa ccgatcacca gcaggctgtc cccgagcacc agggttgcag gtgcgcagag
     1981 atcatcatac acatgatgcc agggtttgag aaaagaacgt ttgatgaaat gccagttcag
     2041 caggtcatga ctccaccagt atccccattg attggtggag aacaggtaat aatccccttt
     2101 gtataaggtg atggtcggat ccgcggtagc ccggtgccgg cccgcttccg agaaatcggg
     2161 gatgggcgtg taaccataat cgatattaat gggattgcaa taggtttgct gctgggcccg
     2221 ggaatgcagg aagaggcaga tcaaacccgg caggaacact aaccttattt tcattcatca
     2281 aaattttaaa tttgattcgc tttattgcct ggcctgaaag tacgggcttt caaaccctaa
     2341 ttttttcaat ccggattcta tttcagggca actcatgaaa agtttccaca acagaccgct
     2401 gcgataattt tcgatcatgc agacgatcgg gccttcatcg atggcgaggt cattccgggc
     2461 ataccagttt ttggtttcat taaatgcgtc ggtgaatccg tacttacccc agatcttatc
     2521 gcccaggtcg taataaaaat gtttgagcgc cttcatggaa tattccggag tatagggaaa
     2581 ggaagaaagg gcggcggacg gggttatggt gcccaggtca tttgtcgggg aatgggcatt
     2641 gtatccgtta taggtatcgc tggccgtaag gccccagcaa ttttctccat accctttgaa
     2701 atgattcggg ttgatcacac aatgtttgta attgatgagg gtatgatgat agttctgtat
     2761 ccagtagtcg gcgtattcgt ctttcagtcc gtgcggatca aggcccataa aactatagtg
     2821 tgcaaaaaac agcggtccgc cgaaatcgaa tcccagggga agggtaatgc catagtagga
     2881 acggccgttt aaaaaatggt tgctctccgc ccagcatctt ctgtacacat ccgccgtgat
     2941 gggataggtg ggggacgaag cggccagcac atacgtgatc aggcattcat tccatcctct
     3001 gagctcaaaa ttcatgctcc atccgttgtt cgggctccag tgccagtaaa aggaattgcc
     3061 gccgtcccgc tggtgccagc tccactcggc ttctctccag agtgagttga tgcggttgcg
     3121 gagttcgttt tccagccgtt cgttccggtt gaaatattgc cgggcacaaa gcagtccttc
     3181 aaacaggtaa gaggtttcca ccacgtcggc gccatcgtct ttccgggaaa agggaatggt
     3241 gaccccggtg gttccgttca tccagtgcgg aaaaatcccg tgataactgt tggccttcat
     3301 taaaaagctg acggttttga gcaggtgttt cacggcggtg tcccggctga tccaatgccg
     3361 gtcggctgcc acgatgatcg ccatgatgcc gaaaccgctg ccgccggtag ttaccacctc
     3421 atcgccataa ccataagcgt tattgctgcg ttcgcgggca agcccgctga gggggtgggc
     3481 aaaatcccag aaataggaaa aggtctgttt ttgcaccagg tccagcagcg cactgtcgga
     3541 aagtaccttt ctttcctccg gggccgccgg attttttgat acgggtttaa cgggctggcc
     3601 gaaagaaaca aataccagac tgaaaaacaa tgcggtcagc catcctgcct tgatagaaag
     3661 atatttcatt ttttaaacgg ctttaatgcg tattgcggtc ctgccttttc ttcagttcag
     3721 tcgggggctc tggaagccaa gcgctttcat tcccgttttt atttcaggac aactcataaa
     3781 caggttccac agcaatcccg accggtaatt ttcaatcatc acgatttcgg gaccctggtc
     3841 aatggccagg taggaatccg cgaaccaggg gtccgataaa ctgaaggcat ccacaaaacc
     3901 atattctccc caaagtttat cacccagcgt gtaataaaaa aatttcaatg cattcatgga
     3961 ttcggttggc gtgtacggca gggaggagat ggcggccgtg ggagagataa ttccaaggtc
     4021 gttggtgggc gagtgggcag aataaccgct gatattatca tcgctggcgg tgaggcccca
     4081 gcagaggttg ctgtacccat agtagcccat cggattggcc acgcaatatt tgtaattgat
     4141 ctgggcatgg gccgtgttct gcgtccagta attggcgtag gcatctgaaa ggccggcagg
     4201 atttatgccg agaaaagaat agtgggcgaa aaaaagcggt cctcccaggg gaggtcccag
     4261 cggcaacgtt attccataat agctgttgcc gtttctcatc gttccgttct gtgcccatcc
     4321 gttgtcataa acggtttttg aaatggcgcc ggcgggggcg gaagcagcga gggcataaac
     4381 gatgagtgct tcatcccagc cactgatctt catgttgatg gcccagccca tgtccgggct
     4441 ccagtgccag tacaaaacat cctgaccgtt ctgccggaac cattgccagt ccaccccctg
     4501 ccagatgctg ttgatgctgt cccggagagc cgtttcatcc ggatcggaag tgttgttgaa
     4561 atattgcctt gcacaaagca atccctgaag catataagag gtctcaacca ggtcggcgcc
     4621 atcgtcctgg gggctgaagg gaacggtttc acccgtggcc ccgttcatcc aatgcgggaa
     4681 agcgccgtgg tagcgttggg ccttggtgct gagaaaatcg acgatcagtc ggaggcgggc
     4741 caggccgctg gcccgggaaa taaaattacg ctgtatgcca acgagcaggc tcatgatgcc
     4801 aaatccgctt cccccggtgg taaccacatc tccggatgaa ttgcgttccc tggccagacc
     4861 ggataccggg tggccgaaat cgtaaaaata tttgaacgtc tgttgctcca cgagggtaag
     4921 caaagcgctg tcggtgaccg gcggaaattt attggt
//
LOCUS       DASYCU010000429         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_93849, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000429 DASYCU010000000
VERSION     DASYCU010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93849"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VGM24_08665"
     CDS             complement(<1..538)
                     /locus_tag="VGM24_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEY4155488.1"
                     /translation="MEKNTIKESSSFKVASKLKDYLLLIKFNLSFMVVFSAVISYLLA
                     PMVTGFDLNAILLLFVGGMLVTGSANALNQALEKDTDAMMKRTEGRPVASGRMSVAKA
                     MSFAIVCGLAGVMLLWHFNFLTAVLALFSLFFYAFIYTPLKKVSSLAVLAGAIPGAMP
                     CLIGWAAGNDNLSSGGWIL"
     gene            complement(564..>1705)
                     /locus_tag="VGM24_08670"
     CDS             complement(564..>1705)
                     /locus_tag="VGM24_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY4155489.1"
                     /translation="TRLPLTIWALLFTAILGVLSFPVLLSGLILLLFDRHFGTSFYLS
                     DIFITATQTALPNEGGSPILFQHLFWFLGHPEVYIIILPAMGLVSEVMAINARKPIFG
                     YMAMVGSLFAITILAFLVWAHHMFVTGLNPFLGSVFVLLTLLIAVPSAIKVFNWLTTL
                     WRGNLRFTPGMLFAIGFVSIFISGGLTGIFLGNSTLDIHLHDTYFIIAHFHLVMGVAA
                     FFGMFCGVYHWFPKMFGRYLNNTMAYIHFWVTLTGAYLIFWPMHYEGLAGMPRRYMDY
                     SGWSSFRQFGGLNQFITDVALVVFAVQLMFVFNFFYSIFKGRKVTTRNPWGANTLEWT
                     TPIRPGHGNWVGEIPEVHRWAYDYGKDGKDFILQTEPLGSDEQHH"
BASE COUNT          490 a          441 c          429 g          345 t
ORIGIN      
        1 aaaggatcca tcctcccgaa ctcaggttat cgtttccagc agcccacccg atcaggcagg
       61 gcatggctcc cggaatggca cccgcgagca cagccagtga actgaccttt ttgagcggag
      121 tataaataaa cgcatagaaa aaaaggctaa acagcgccaa taccgctgta agaaaattaa
      181 aatgccacag cagcataacc cccgcaagtc cgcaaacgat ggcaaaagac atcgctttgg
      241 ccacagacat tcttcccgaa gccaccggcc ttccttccgt gcgtttcatc atggcatcgg
      301 tgtctttttc caacgcctga ttgagcgcat tggcgcttcc ggtcaccagc atgcctccga
      361 caaagaggag caggatggca ttgagatcaa atcccgtaac catcggcgcc agcaggtagc
      421 tgatcacggc tgaaaaaaca accatgaagc tgaggttgaa cttgatcagc agcagatagt
      481 cctttaattt cgacgctact ttaaaagaac tggattcttt aatcgtgttc ttttccatga
      541 atggatagga tttatttccc gggctagtga tgctgttcat cggatcccag aggttctgtt
      601 tgcagaataa aatcctttcc gtccttacca taatcgtaag cccaacggtg tacttccggt
      661 atttccccga cccagtttcc gtgtcccggc cttatggggg ttgtccattc cagggtgttc
      721 gcaccccagg gattcctcgt cgtcaccttt cttcctttga atatggaata gaaaaagttg
      781 aatacaaaca tcagctggac ggcaaaaacc accagggcca cgtcggtgat gaactgattc
      841 agtccgccga actgacggaa ggatgaccat ccgctatagt ccatgtaccg gcggggcata
      901 ccggcaagcc cttcataatg catcggccag aagatcaggt aagcgccggt cagggttacc
      961 cagaaatgga tatacgccat ggtgttgttg agatatctgc cgaacatttt cggaaaccag
     1021 tgataaactc cgcagaacat accaaagaaa gcggcgacac ccataaccag gtgaaaatgc
     1081 gcaatgataa aataggtatc gtgtaaatgg atatccagcg tggagttacc caggaagata
     1141 cccgtcaacc ccccggaaat aaaaatgctg acaaacccga tcgcgaagag catgcccggc
     1201 gtaaatcgca ggttgccccg ccacagggtg gtgagccagt tgaacacttt gattgccgaa
     1261 ggaacggcga tgagcagggt aagcaggacg aataccgagc ccagaaaggg attcaacccc
     1321 gtcacaaaca tatggtgcgc ccaaaccaga aacgcgagga tggtgatagc aaacagggag
     1381 ccgaccatcg ccatgtatcc gaagatcggt ttccgggcgt tgatggccat gacttccgac
     1441 accaatccca tggccggcag aataataatg tatacctccg gatgacccaa aaaccagaac
     1501 agatgctgga aaaggatcgg gcttccgcct tcgtttggta aagcggtttg tgtggcggta
     1561 ataaaaatat cagacaggta aaaactggtt ccgaaatgcc ggtcgaacaa caggagtatc
     1621 aggccggaga gcagtaccgg aaacgacaga acgccgagga tagccgtaaa caggagggcc
     1681 cagatggtca gcggcaaacg ggtca
//
LOCUS       DASYCU010000430         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4588908, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000430 DASYCU010000000
VERSION     DASYCU010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4588908"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2344
                     /locus_tag="VGM24_08675"
     CDS             <1..2344
                     /locus_tag="VGM24_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY4155490.1"
                     /translation="MWGTIAGFILKFRLSLLLLLGLACVFAAYEASKVELSYEYTRAI
                     PTDNPKYLAYQDFLRKFGDDGNLLVMGIQTGKLFTRDFFNDYIRLNKGLKKVKGVEQV
                     LSIPDAILLIKNDSTQKLIPKPLFPSTFNTQKELDSAKNAFENQVFYRNLLYNPATHT
                     YLAAIHVNKQVMNSAARTGVVAQIVQLGDEFGKAHQVQMHYSGLPLIRTNLATKVASE
                     MKWFLLGSVLLSALILLLFFRSFSTMLLSLLVVITGVVFSLATMHLFGYKISLLNALI
                     PPLIVVIGIPNCIYFLNKYHTSFTENREKKKALYEMIRKMGIVTLFCNIAAAIGFGVF
                     AFTKSAILKEFGVVAGINILLLFFISILLLPAALSYLPDPKTRHMNYLTNRWLTSILD
                     RLEQWTVHHKAVIYLTTTAILIFSVAGILRLKSIGFILDDLPKTDPVYTDLKFFEQNF
                     KGVMPLEILIDTKRKNGLRANMMGTFEKIEKLSDFIDSSPVMARPLSIVEGLKFVRQA
                     YYHNDSTDYGLPNEFDISFLAPYLNTKADSSKSNNVMKLARSFIDSNNQTTRISVNMA
                     DVGSVRLPKILQSIQQRASRLFDPSKYSVELTGTTVTFLEGSAFIIHGLKQSILWAFV
                     LIAACMLYLFRSLRILICSLLPNLIPLVITAGVMGWAGVPLKPSTVLVFSIALGIAID
                     ITIRFLVNYKQDQGKGSVKEAVIRTIHSTGISIIYTSLVLIAGFVIFCFSGFGGTQAL
                     GWLTSLTLVMATLTNLLFLPALLISISRKKKPEVPSSTAL"
     gene            complement(2325..>2876)
                     /locus_tag="VGM24_08680"
     CDS             complement(2325..>2876)
                     /locus_tag="VGM24_08680"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY4155491.1"
                     /translation="RSFSPAELDTAMQSLKTRYPNNPFLAEMEKNSKAQQQAEQSSGA
                     SWVGKTVPELSLPDVNGKPVSVNSFKGKYLLIDFWASWCGPCRMENPNVVEAYKEFKG
                     KNFTILGVSLDKDRDSWKKAIAQDHLTWTHISDLKYWGSQAVSTFGFQGIPFNVLVDP
                     SGKIIAQELRGRDLENKLKELLN"
BASE COUNT          721 a          723 c          674 g          758 t
ORIGIN      
        1 tatgtgggga actattgccg gttttatact gaaattccgt ttatcgctat tattattact
       61 cggtcttgcc tgcgtttttg ctgcctatga agcgtccaaa gtagaattaa gctatgagta
      121 tacgcgggcg atcccgacgg ataacccgaa atacctcgct taccaggatt ttctgagaaa
      181 attcggtgac gatggaaacc tgctggtgat gggtatccaa accggaaaat tatttaccag
      241 agattttttc aacgattata tccggctcaa taaaggacta aaaaaagtaa aaggggtaga
      301 acaggtcctc agcataccgg atgccattct gctgatcaaa aacgacagca cgcaaaaact
      361 gatacccaaa ccgttatttc cctccacctt caatacccag aaagaactgg atagtgcaaa
      421 aaatgctttt gaaaatcagg tgttctaccg caacctgctt tacaaccccg ccacacacac
      481 ctatctcgcc gccattcacg tgaacaaaca ggtgatgaat tccgctgcga gaacaggcgt
      541 ggttgcacaa attgtacaac tgggagatga gtttgggaag gctcatcagg ttcagatgca
      601 ttacagcgga cttccgctca tccggaccaa cctcgccacc aaggtggctt ccgagatgaa
      661 atggttcctg ctgggctccg ttttactctc ggcgctgatc ctgctgctct ttttccggag
      721 tttcagcacc atgttgttat cgctgctggt cgttatcacc ggcgtggtat tcagcctggc
      781 caccatgcac ctcttcggat acaagatcag cctgctgaat gccctgatcc cgcccctgat
      841 tgtggtgatc gggattccca attgtatcta tttcttaaat aaataccata cttccttcac
      901 ggaaaacagg gagaagaaaa aggcattgta tgaaatgatc cggaaaatgg gtatcgtaac
      961 cctgttctgc aatatcgcag ccgccatcgg ctttggcgta ttcgccttca ccaaaagcgc
     1021 catcctgaaa gaattcggag tagtggcggg aatcaatatt ctgctgctct tttttatttc
     1081 gatcctcctg ctgccggcgg cgctgagtta tttgcccgat ccgaagaccc ggcacatgaa
     1141 ttacctgacc aaccgctggc tgaccagcat tctggaccgg ctggagcaat ggaccgtcca
     1201 tcacaaagcc gtcatttacc tgaccacgac cgccatattg atattttcgg tagcgggaat
     1261 actgcgcctg aaatccatcg gatttattct cgacgatctt ccaaaaacgg atccggtata
     1321 cacggatctc aaattcttcg aacagaattt taagggcgtg atgccgctgg aaatattgat
     1381 cgatacgaaa agaaaaaacg gactgcgggc gaacatgatg gggacttttg aaaaaataga
     1441 aaagctttcc gatttcattg attccagtcc ggtgatggcg agacctttat ccattgtgga
     1501 agggctgaaa ttcgtacgcc aggcttatta ccacaacgac agcacggact atggcctgcc
     1561 aaacgaattt gacatcagct ttctggctcc ttacctgaat acgaaggccg acagcagcaa
     1621 atccaacaat gtcatgaaac tggccaggag ctttatagac agtaacaatc aaacaacccg
     1681 gatcagcgtg aacatggccg acgtcggcag tgtacgcctg cccaaaatcc ttcagtcgat
     1741 acaacaaagg gcttcccgtt tatttgaccc ttcaaagtat tctgttgaac tcacgggaac
     1801 tacggtcact ttcctggaag gaagtgcctt tatcatccac ggactgaagc aaagtatttt
     1861 atgggctttt gttctgatcg ccgcatgtat gctttatctg ttccggtctt tgcggatcct
     1921 gatctgttcc ctgcttccaa accttattcc cctggtgatc accgccggcg tgatgggctg
     1981 ggccggcgtg ccattaaagc cttccacggt gctggtattc agtatagctc tgggtattgc
     2041 cattgatatt accattcggt ttctggtaaa ttacaaacag gatcagggaa agggcagcgt
     2101 aaaagaagct gtgatccgaa cgatccattc cacgggcatc agcatcattt atacttccct
     2161 ggtgctgatt gcggggtttg ttatattctg tttcagcgga ttcggaggca cccaggcatt
     2221 gggttggctg acttcactta ccctggtgat ggctacgctt acgaacctgc tgtttttacc
     2281 ggccttgctc atttccatca gcaggaaaaa gaagccggag gtccctagtt caacagctct
     2341 ttaagcttat tttccagatc cctgccgcgg agttcctgag cgatgatttt tccggaagga
     2401 tctaccagca cgttaaaggg aataccctgg aaaccgaagg tcgaaacggc ctggctgccc
     2461 cagtatttca ggtcactgat atgggtccag gtaaggtggt cctgggcaat ggcttttttc
     2521 caggaatccc tgtccttatc cagcgatacg cccaggatag tgaaattttt ccctttgaac
     2581 tccttatacg cttccaccac atttggattt tccatgcggc agggaccgca ccagcttgcc
     2641 caaaagtcga tgagcagata tttgcctttg aaagaattaa cagataccgg tttgccgttc
     2701 acatccggga gactgagttc aggaacggtt ttgccaaccc aggaggcgcc ggatgactgc
     2761 tccgcctgtt gctgggcctt gctgtttttt tccatctccg ccagaaaggg attgttggga
     2821 tatctggttt tcagactttg catggccgta tccagttccg caggcgaaaa actgcg
//
LOCUS       DASYCU010000431         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2411988, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000431 DASYCU010000000
VERSION     DASYCU010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411988"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="argS"
                     /locus_tag="VGM24_08685"
     CDS             complement(<1..1018)
                     /gene="argS"
                     /locus_tag="VGM24_08685"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY4155492.1"
                     /translation="MDLVPRIKQAVQLALDQKYQYRTDVDDILVNETKPEFTGDYTVV
                     LFSISKILKSNPDALGSGLGEALLSAHPGLFAGFNVIKGFLNLVIGEGILTGFLQDHY
                     ADSAYGIRPANGRRVMVEYSSPNTNKPLHLGHLRNNFLGWSVASLYQANGYEVIKTCI
                     ANDRGIHICKSMLAWKLYAGGATPESTGIKGDHFVGDYYVLFGNELKKQIAERIAAGL
                     SREQAEKEAPLMKQAQEMLVAWEAGHPETIALWEKMNSWVYAGFDITYKRIGSDFDKI
                     YYESETYLLGKDLVEEGIRKNVFYRKDDGSVWIDLREDGLDEKIVQRSDGTAVYMTQD
                     IGLAV"
     gene            1106..1603
                     /locus_tag="VGM24_08690"
     CDS             1106..1603
                     /locus_tag="VGM24_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY4155493.1"
                     /translation="MPKKQIISFIDFFYPPFRKLMPIQTFRYAACGGANTLLDIFAFF
                     ISYNFILHKHVLHIGFLAFKPYIAAFIMSFMVSFPTGFLLMRHVVFPGSSLHGRVQLF
                     RYFLLVVICILLNYIFIRLFVEKFNIYPTIAKIITTSIVVFFSYLTQKHYTFKTNDGT
                     LNAER"
BASE COUNT          378 a          460 c          333 g          449 t
ORIGIN      
        1 gaacagcaag gccgatatcc tgcgtcatat aaacggcggt tccatcgctc cgctgaacga
       61 tcttctcatc caggccatct tcccgcagat cgatccagac gctgccgtcg tcctttcggt
      121 aaaacacatt tttacggatg ccttcttcca ccaggtcctt acccagcaga taggtctcgc
      181 tttcataata gattttatca aaatcacttc cgatccgctt ataggtaata tcaaaaccgg
      241 catataccca gctgttcatt ttttcccaca aggcaatggt ttccggatgg ccggcttccc
      301 aggctaccag catttcctgg gcctgcttca tcagcggcgc ttctttttcc gcctgctccc
      361 tagacagtcc ggcggctatt ctttccgcta tttgtttttt caattcgttc ccgaacagaa
      421 cataataatc gcccacaaaa tgatcccctt tgatcccggt gctttccggc gtggcgcccc
      481 cggcatataa tttccaggcc agcatgcttt tgcagatgtg aatgccccgg tcattggcaa
      541 tacaggtctt aatgacctca tatccattgg cctgatacag ggaggccaca ctccatccga
      601 gaaaattatt tctcaggtgg cccaggtgca ggggcttgtt ggtattgggg gatgaatatt
      661 caaccatcac ccggcggcca ttggcaggcc tgatcccata ggcggaatcc gcataatggt
      721 cctgcagaaa gcccgtcagg attccttccc cgatcaccag gttcaaaaac cctttaatca
      781 cgttaaaacc ggcaaataaa cccggatgag cgctcagcag ggcttccccg agcccgcttc
      841 ccagcgcatc cggatttgat tttaatattt tactgatgga aaacaggacg acggtataat
      901 cgcccgtaaa ctccggcttg gtttcattga ccaggatatc gtcaacatcc gtccggtact
      961 ggtatttctg atccaatgcc agctgcactg cctgtttgat ccggggaaca agatccatcg
     1021 tcataaattg accgcaaaag taggacatgt tgggtagact atatcagtcc tttgtagtaa
     1081 cttcgcgcgg gaattagcgg attttatgcc aaaaaagcag atcatttctt ttattgactt
     1141 tttctacccg cccttccgaa agctgatgcc catacagact ttccgctacg cagcctgcgg
     1201 tggcgccaat accctcctgg atatctttgc ttttttcatc agttataatt ttatcctgca
     1261 caagcacgtg ctccatatcg gatttcttgc cttcaaaccg tacatcgcag catttatcat
     1321 gtcctttatg gtaagcttcc caaccgggtt tcttctcatg cggcatgtgg tattccccgg
     1381 gtccagcctc catggcaggg tccagctctt ccggtatttt ttgctcgtgg tcatttgcat
     1441 cctgctgaac tacatcttca tccgcctatt cgtagagaaa ttcaatatct atcccaccat
     1501 tgcgaaaata ataaccacct ctatcgtcgt gttcttcagt tacctgacac aaaagcatta
     1561 tacgtttaaa accaacgacg gcacgctgaa cgcggagcgc tgaaaaccgt catgtactcc
//
LOCUS       DASYCU010000432         6741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1979249, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000432 DASYCU010000000
VERSION     DASYCU010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979249"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VGM24_08695"
     CDS             complement(<1..159)
                     /locus_tag="VGM24_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY4155494.1"
                     /translation="MIREAIVLAGGLGTRLKSVVPDLPKCLAPVAGRPFLEYLLACYR
                     KQGIRRFIF"
     gene            complement(171..749)
                     /locus_tag="VGM24_08700"
     CDS             complement(171..749)
                     /locus_tag="VGM24_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEY4155495.1"
                     /translation="MPEDIKRIISASIAVKQKLLADENLLKTTQSVVDRVVKAFKNGN
                     RVYFAGNGGSAADAQHLAAEFSGRFYTDRHALPAEALHCNSSYLTAVANDYSYDEIYA
                     RMIKGIAHAGDVLIGLSTSGNSKNIVRAFEAAREKGVVTIGFTGENGGQMKALSDYLF
                     NVPSGDTPRIQESHILLGHIVCELVERDFFKV"
     gene            complement(761..1795)
                     /locus_tag="VGM24_08705"
     CDS             complement(761..1795)
                     /locus_tag="VGM24_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEY4155496.1"
                     /translation="MAYPMIYRSKAPLRIGLAGGGTDVSPYSDLYGGAILNATVSLYA
                     YANIELTENDELQFEAIDFQTKQVIQKNSPVELNGVLDLHKGVYDRIRKQFQEIPTGF
                     KLSTFVDAPAGSGLGTSSTLVVAMVGAFAEMLKLPLGEYDIAQLAYEIERKDLRMAGG
                     RQDQYAATFGGVNYMEFYDKEKVIVNPLRVKQQYLFELENNLVLYYTATSRESAKIIA
                     KQSQNVIDKKGSSIEAMHQLKQQSQRMKEALLRGRLNEIGEILDFGFEQKRRMAEGIS
                     TDRVEDIYQAAKAAGATGGKISGAGGGGFMIFYCPGNTKYPVIEKLNSFGGHCKSYQF
                     VGHGLTTWTI"
     gene            complement(1767..2918)
                     /locus_tag="VGM24_08710"
     CDS             complement(1767..2918)
                     /locus_tag="VGM24_08710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4155497.1"
                     /translation="MKVVIIGPAYPLRGGLATFDQRLCKAFSDEGHAASIYSFSLQYP
                     GFLFPGTTQYSSDPPPENTEIHTLINSVSPLNWLFTGNRLRKEAPDLVVVRYWLPFMG
                     PALGTILRRVRKNRKTRIIAITDNVLPHEKRPGDLLFTKYFLKACDAFVSMSDEVMRD
                     LRKLEKQKPAEKVIHPLYDNFGEIIPKKNARKILRERFHLDVTDGERIVLFFGFIRKY
                     KGLDILLRAMAEPGIRESGIRLLVAGEYYEDEKIYRELIARLRIPDLLILKTDFIPDN
                     EVQYYLCAADAVIQPYRHATQSGVTPLAYHFERPMLVSNVGSLPSHVIHDQTGLVAEP
                     EPASIARAMLRFYELGEDYFIPHLRMEKKKYSWKNLVNTILGLSHDIQK"
     gene            complement(2915..3853)
                     /locus_tag="VGM24_08715"
     CDS             complement(2915..3853)
                     /locus_tag="VGM24_08715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4155498.1"
                     /translation="MDLSIIVPVFNEEESIPELCDWIAKVMERNFLSYEAILVDDGST
                     DRSWAVIESIHEKNDRVKGIRFQRNYGKSAALNEGFRSCLGDVVITLDADLQDSPEEI
                     PALRNMILNDGFDLVSGWKKVRHDPLSKTIPSRFFNWVTSKVSKIKLHDFNCGLKAYR
                     LKVVKSIEVYGEMHRYIPLIAKWAGFRKIGEKVVEHHPRKYGESKFGMGRLITGGLDL
                     ASILFVGKYEKRPMHLFGSWGIVSFLVGFIIFFYLFLSKIFFDQTGLTQRPLFFVALV
                     GMIIGTQLFVTGFIAELISRNSLNRNAYLVEKRIGL"
     gene            complement(3860..4399)
                     /locus_tag="VGM24_08720"
     CDS             complement(3860..4399)
                     /locus_tag="VGM24_08720"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HEY4155499.1"
                     /translation="MQPISKYNTGLRFGLITGLIYAVLLFLRYNFFAGSPIAFGLFAI
                     VSYLAILILYLFAGISRKKEFGGYADFKDIFQTIFLTILITEAVYVIFNAIYLKYVDP
                     SFFDNFKVVTRNFFEKAGMTQDQIDSKMKGFDEANKEMTPWALVKGFGTWVIIDSIIG
                     MIYSSILRKKKDIFQEPKL"
     gene            complement(4415..5683)
                     /locus_tag="VGM24_08725"
     CDS             complement(4415..5683)
                     /locus_tag="VGM24_08725"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY4155500.1"
                     /translation="MKLLIKQACITDPLSSHQGTIRDILIEHGVIREIAAYIADKDSE
                     KIINAEGLFVSPGWVDIFSSFAEPGYEFKETLETGSRAAAAGGFTDVFLIPDTNPVTD
                     SKTQVEYLRQRSASLPVNIWPIGAITRNLEGKHLAEMYDMQNSGAVAFSDGLHPVQSA
                     GVLVKALQYVKAFDGVIIQIPDDTSIAPNGLMHEGIVSTRLGLPGKPLMSEELIIARD
                     IKLTRYTDSHIHFTGVTSPKSIEYIRRAKASGLQVTCSVTPYHLFFSDEDLQKYDTNL
                     KVYPPLRPKAVSGPLIEALRDGTIDCICSHHLPHEYDSKELEFEHAKYGMTGLETAYA
                     AVQTLFPDLSPGKIYALFGGNARKIFKLDQPRIEINETAQMTLFNPAEEWQFKLSQSK
                     SKSRNSAFQDHIFTGKPKGIIRQERLFINE"
     gene            5778..5851
                     /locus_tag="VGM24_08730"
     tRNA            5778..5851
                     /locus_tag="VGM24_08730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5812..5814,aa:Pro,seq:ggg)
     gene            6037..>6741
                     /locus_tag="VGM24_08735"
     CDS             6037..>6741
                     /locus_tag="VGM24_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155501.1"
                     /translation="MRYTFHKFFFITGLCALVLAGCQQAEKKMDRTRSFNDGWRFTRD
                     SVSGAEQPGFDDSKWRMLDIPHDWSIEDLPAQVDGKTTGPFSKESPGGAATGHTIGGT
                     GWYRKTFTLQNEDGGKRISVYFEGVYMESDVWINGNHLGTHPYGYTSFYYDISKYCKP
                     AGQPNILAVRVINNGKNSRWYSGSGIYRNVWLTVTRPLHIDPWGVYITSPEVSVDSAA
                     VHVQTSILNEESRKQDI"
BASE COUNT         1672 a         1705 c         1682 g         1682 t
ORIGIN      
        1 aaagataaac cggcggatgc cctgtttccg gtaacaagcg agcaggtatt cgagaaaggg
       61 tctgccggca accggtgcaa ggcatttggg cagatccggc acgacggatt tcagccgggt
      121 accaagccct ccggcaagaa cgatggcctc cctgatcatg cgcgcttgat ttagacttta
      181 aaaaaatctc tttccacgag ttcacaaaca atatgaccca gcaggatatg cgattcctgg
      241 atacgcgggg tatcgccgga aggaacatta aaaagatagt cactgagggc tttcatctga
      301 ccgccgtttt ctccggtaaa tccgatggtg acgacgcctt tttcccgcgc cgcttcaaat
      361 gcgcgcacaa tatttttgga attgcccgag gtggataatc cgatcagcac atcccccgcg
      421 tgggcaatcc ccttgatcat ccgcgcataa atttcgtcat agctgtaatc attggccacc
      481 gccgtcaggt aggaggaatt gcaatgcagc gcttcggccg gcagggcatg acggtcggtg
      541 taaaaccttc cggagaattc tgccgccagg tgctgtgcat ccgcagcgct tccgccgttg
      601 ccggcgaaat aaacgcgatt gccattttta aatgccttca ccacccggtc caccacggat
      661 tgcgtggtct tcaggagatt ttcatctgcc agcagttttt gttttacggc aatggaagcg
      721 gaaataattc gtttaatgtc ctccggcatg atgttaaatt ttaaatggtc caggtggtca
      781 gaccgtgacc aacgaactga taagatttgc aatgtccccc aaaggaattc agtttttcga
      841 tcacggggta tttggtattt cccgggcaat aaaagatcat gaacccgccg ccgccggcgc
      901 cggaaatttt tccgcccgta gcgcccgccg ccttcgccgc ctggtaaata tcctccacgc
      961 gatcggtgga gatgccttct gccatccggc gtttttgttc aaacccgaaa tccagtatct
     1021 ccccgatttc attcagccgc ccgcgcagca gggcctcctt catgcgctgc gactgttgct
     1081 tcagctgatg catggcttcg atggaagaac cttttttatc gatcacattc tgactctgtt
     1141 tcgctatgat ctttgccgac tcgcggctgg tggcggtata ataaagcacc aggttatttt
     1201 ccagctcaaa taaatattgc tgttttacgc gcagggggtt cacgatcact ttttccttat
     1261 cgtaaaactc catgtagttg accccgccaa aagtggccgc atattggtcc tgacggcctc
     1321 cggccatccg gagatccttc ctttcgattt cgtacgcgag ctgcgcgata tcatattccc
     1381 ccaggggcag tttcagcatt tccgcaaagg cccccaccat cgccacgacc agcgtggacg
     1441 aagtgcccag tcccgacccg gccggggcat ctacaaaagt ggacaattta aaaccggtcg
     1501 gaatttcctg gaattgcttt ctgatccggt cgtaaacgcc tttgtgcaaa tcgagcaccc
     1561 cattcaactc tacgggcgaa tttttttgaa tcacctgttt cgtctggaaa tcgatcgctt
     1621 caaactgcag ttcgtcgttt tccgtcaatt cgatattggc ataggcataa agggaaacgg
     1681 tggcattgag gatggcgcct ccgtaaagat cactgtaggg actcacatcc gttccgccac
     1741 cggccagtcc gatgcgaagc ggcgccttac ttctgtatat catgggataa gccaagaatg
     1801 gtatttacaa ggttcttcca ggaatatttt ttcttttcca tgcgaagatg aggaataaaa
     1861 taatcttctc ctaattcata gaagcgcagc atggcgcggg caatggatgc cggttcgggt
     1921 tcggcaacca gcccggtctg atcatggatg acatgcgaag gaaggcttcc cacattactt
     1981 accagcatgg gccgttcaaa atgataggcc aggggagtaa ctccactttg cgtggcgtgg
     2041 cggtaaggct gaatgaccgc atctgcggca cataaataat actgcacctc attatccggg
     2101 ataaaatccg tttttaaaat aagaagatcc gggatccgga gccgggcaat gagttcccgg
     2161 tatatttttt catcctcata atattctccg gccaccaaaa gccggatccc ggattcccgg
     2221 atacccggct cggccatggc gcgaagcagg atgtccagcc ctttgtattt gcggatgaac
     2281 ccaaaaaaca gaacgatcct ttctccatcg gtcacgtcca ggtgaaatcg ctctctcaat
     2341 attttccggg catttttttt tggaatgatc tctccaaaat tatcgtacag gggatggatg
     2401 actttctccg ccggcttttg cttttcaagc tttctcagat cccgcatcac ctcatcgctc
     2461 atgctgacga aggcatcaca ggctttcaga aaatatttgg tgaatagcag gtccccgggg
     2521 cgtttttcgt ggggcaacac attatccgtg atggcgatga tgcgtgtttt cctgttcttc
     2581 ctgacccgcc gcagaatcgt gcccagcgca ggacccataa agggcagcca gtaacggacg
     2641 accacgagat ccggcgcttc ctttctcagc cggttcccgg taaacagcca gtttaacgga
     2701 ctaacggaat tgatcagggt atggatctcc gtattttccg gtggcggatc ggatgaatac
     2761 tgggtcgttc ccggaaaaag aaatccggga tattgcaggg aaaaagaata gatggaagca
     2821 gcgtgccctt cgtctgaaaa ggctttgcat aaacgctgat caaaggtagc gaggccgccg
     2881 cgcaggggat aggcaggacc gatgataacc actttcatag tccgatccgc ttttcaacca
     2941 ggtaagcatt tctgttcagg gagtttcggg agatgagttc ggcaataaaa ccggtaacaa
     3001 ataattgtgt accgatgatc atgcccacga gggcaacaaa aaacagcggt ctttgggtaa
     3061 gcccggtctg gtcaaagaaa atttttgaca ggaacagata gaaaaaaatg atgaagccga
     3121 cgagaaagga aacgatcccc caggatccga ataaatgcat gggccgtttt tcatatttac
     3181 caacaaacag aatagatgcc aggtccaggc ccccggtgat cagccggccc atcccaaatt
     3241 ttgactcgcc atacttcctc ggatgatgtt ccaccacttt ctcgccgatc ttccggaacc
     3301 ccgcccattt tgcgatcaac ggaatatagc ggtgcatctc gccgtacact tcgatgcttt
     3361 tcaccacttt taaccggtaa gcttttaatc cgcagttaaa atcatgtaat ttgatcttgg
     3421 agactttgga ggtgacccag ttgaaaaagc gggagggaat ggttttggat aaaggatcgt
     3481 ggcgcacttt tttccagccg ctcacgaggt caaatccgtc attcaggatc atgtttctta
     3541 aagccggaat ttcctccgga ctatcctgca gatcggcatc cagcgttatg accacgtctc
     3601 ccaggcagct gcggaagcct tcattgaggg cggccgactt gccgtagttg cgctgaaaac
     3661 ggatgccttt gacccggtca ttcttttcat gaatggattc gatcaccgcc caggaacggt
     3721 ccgtgctccc gtcatccacc aggatcgcct catagctcaa aaaattccgc tccatcactt
     3781 ttgcgatcca gtcgcagagt tcaggaatgg attcctcttc attaaaaacc ggaacgataa
     3841 tggaaagatc catgagcgat tacaacttgg gttcctggaa aatatctttt ttctttctca
     3901 gaatcgagga gtagatcata ccgataatac tatctataat aacccaggtg ccaaatccct
     3961 taaccagcgc ccagggagtc atctccttgt ttgcttcatc aaagcccttc atcttactgt
     4021 cgatctggtc ctgcgtcatg ccggcttttt caaagaaatt ccgggtgacc accttgaaat
     4081 tatcgaagaa ggaagggtcc acatatttta ggtaaatggc gttaaagatc acataaacgg
     4141 cttccgtgat taatatggta agaaagatcg tctgaaaaat atccttgaaa tcagcataac
     4201 ccccgaattc ttttttccgg gatattccgg cgaataaata aaggatcaga atagcgaggt
     4261 aagagacaat ggcgaacagg ccaaacgcaa tcgggctgcc cgcaaaaaaa ttataccgca
     4321 gaaaaagcag gacggcgtag atcaggccgg tgatcagccc gaaacgaagg ccggtattgt
     4381 atttagaaat tggctgcata aagaggattt taaattattc atttatgaaa aggcgttcct
     4441 gccggatgat gcccttcggt ttccctgtaa agatatgatc ctggaacgca ctattcctgg
     4501 atttggattt tgattgcgat aatttaaact gccattcttc ggccgggttg aaaagggtca
     4561 tctgggccgt ttcatttatt tcaatccggg gttgatccag cttaaaaatt tttctggcgt
     4621 ttccgccaaa caaggcatag atttttcccg gggaaaggtc gggaaagagg gtctgcacag
     4681 ccgcataggc cgtttccagt ccggtcattc cgtatttggc atgctcaaat tccaactcct
     4741 tgctgtcgta ttcgtgcgga aggtgatggg aacagatgca gtcgatcgtg ccgtcccgga
     4801 gcgcttcgat caatgggccg gagaccgctt tgggccgcag gggaggatat accttcagat
     4861 tggtatcgta cttctgcaga tcctcatcgc tgaaaaaaag atgatatggc gtaacggaac
     4921 aggtaacctg aagcccggac gctttcgccc tccggatata ttcaatggat ttgggagagg
     4981 taacgccggt aaaatgaata tgggaatcgg tgtaccgggt cagtttgata tcccgggcaa
     5041 tgatcagttc ttcagacatc aggggcttgc cgggaaggcc aagccgggtg gaaacaattc
     5101 cctcatgcat caggccgttc ggagcaatgc tggtatcgtc ggggatctgg atgatcacgc
     5161 catcaaatgc tttgacgtat tgaagcgctt tcaccagaac acccgcagat tgaaccggat
     5221 gcaggccatc ggaaaaagcc acggccccgc tgttttgcat gtcgtacatt tcagcgaggt
     5281 gtttcccctc caggttgcgg gtgatggcgc cgatcggcca gatattcacc ggcagtgaag
     5341 cggatcgctg acgcaggtat tccacctggg tcttcgaatc ggtcaccggg tttgtatccg
     5401 ggatcaggaa aacatcggta aatccgccgg ccgccgcagc gcgtgaacct gtttccaggg
     5461 tctccttaaa ttcataaccc ggctctgcaa aactggaaaa aatatccacc catccgggcg
     5521 agacgaaaag gccttctgcg tttattattt tttctgaatc cttgtccgca atataggctg
     5581 caatctcgcg gatcacgcca tgttcgatca atatatcccg gatagttccc tgatgggatg
     5641 agagcggatc agtaatgcaa gcctgtttga ttagaagctt catctataag gaattgcgcg
     5701 aagatacggt ttacctttta aatgcatttt atggcaaggg atttttatca tagatttgca
     5761 gcccctccgg gggaattcgg gacgtagcgc agcccggtag cgcgtttgca tggggtgcaa
     5821 gaggccgctg gttcgaatcc agtcgtcccg accgagacag gacctgctga attgaaatca
     5881 gctcgtcctg tttttatttt ggttcaaccc ccgtcgttca aacactaagg ccgccggtcg
     5941 gtgccgctaa ttaactcgtc tcctttgcct aaattcgctg atgacccgaa attccaaggc
     6001 cttacttccc gccattgaac ctgcaataac ctattgatga gatatacatt ccacaaattt
     6061 ttttttatca ccggactgtg tgccctggtc ctcgcgggct gtcagcaggc agagaagaaa
     6121 atggacagga cgcgctcttt caatgacggc tggcggttta cccgggactc tgtttccgga
     6181 gcggagcaac cgggttttga cgattcaaaa tggaggatgc tggacatccc gcacgactgg
     6241 agcatcgagg atctgccggc gcaggttgac ggaaaaacaa ccgggccttt ttcaaaagaa
     6301 agcccgggcg gcgcagccac cggtcatacc atcggtggaa ccggctggta ccggaaaaca
     6361 tttaccctgc agaacgagga tggcggaaag cggatatcag tttatttcga aggagtctac
     6421 atggagagcg atgtctggat caatggaaat cacctgggga cacaccctta tggttatacc
     6481 tctttctatt atgatatttc caaatactgc aagcctgccg ggcagccaaa tattctggcc
     6541 gtccgcgtaa tcaacaatgg aaagaacagc cgctggtaca gcggttcggg tatttaccgg
     6601 aacgtatggc tgacggttac ccgtccgttg catattgacc cctggggagt ttatattacc
     6661 agtccggaag tttccgttga ttcagccgcc gtgcacgttc agacaagtat cttaaacgag
     6721 gaaagccgga aacaggatat t
//
LOCUS       DASYCU010000433         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2848537, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000433 DASYCU010000000
VERSION     DASYCU010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2848537"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2113)
                     /locus_tag="VGM24_08740"
     CDS             complement(<1..2113)
                     /locus_tag="VGM24_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HEY4155502.1"
                     /translation="MPQTLIAASRPDFETRFVFFRRKRMFITGFLLLSAIGVLRAQKS
                     AFLREYEKTFTSYPFSDPDPIPNPAAIIYPYFRYDGFTDKPVQKKWKVVELENAYLKI
                     TILPEVGGKIWSAIEKSTGKSFIYNNHVVKFRDVAMRGPWTSGGIEANYGIIGHTPNC
                     ATPVDYSTFTRPDSSVSCVIGVLDLLTGSKWRLEINLPADKAYFTTTSFWYNGTPLEQ
                     PYYSWMNTGIKAKGNLQFIYPGTHYLGHEGEYSDWPVNKQNGRDISFYENNNFGGYKS
                     YHVFGKYSDFYGAYWHDDDFGMGRYSTHDDKAGKKIWIWGLSQQGMIWEHLLSDTDGQ
                     YVEVQSGRLFNQSAEGSSFTPFKHLGFAPYAADRWTEYWFPVKQTRGFVKANPYGALN
                     IKKENGWLKIFFSPLQKIHDQLKITAAGKMIYSKELQFNTLQTFADSISTDVNAENIE
                     ATLGENKLIYRSSPDADTLSRPLETPAHFDWNSVYGLYLQGKEDIRERAYPAAAAKLR
                     ACLAGDPNYLPALDELGSLQYRNLEYTEALASLKRALSIDTYDPSANFNYGLTNLKLG
                     HEADAKDGFDIATLSVEYRSPAYLELGKIYFRKKEFARALDYAAKSLTGNQDNLDAYR
                     LEAMIYRLQNRESEAMQILDKIAAMDPLSHFSRFEKYLWHPSSGAGKYFTSLINNEMP
                     EETFLDLGIWYYNLNLKEEALK"
     gene            complement(2130..3365)
                     /locus_tag="VGM24_08745"
     CDS             complement(2130..3365)
                     /locus_tag="VGM24_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY4155503.1"
                     /translation="MPYTIFRHLNGHSLSHVALKDLSLKKNVVAFLTEKGESTMLDLA
                     DQLNISVPKTTELISGLASEGLVKATGRKTEGLGRKAAIYTLNPVSCYFLGIEIRKYE
                     INIGLMSFNAGMVETALGIPFLFSDPDESLNAIVNEIQAFLAKSIIPKDKIIGLGLSL
                     SGRINVKTSQILSNYHFAEAPIKSRLEEVLGLPVYIDNDSRTIAYGEYYFGRDGLSYP
                     EKNVCIVNLDYGMSLGIFVDGRPVYGTSGYAGEIGHIPMYNNEKICFCGKKGCLETEA
                     SGLALIDFINSKMMKGSSSRLQKTLAKKGYLQLENILEAVLHGDSLAIEGVTEIARNL
                     GKGLAVAINMLNPDLIVLGGLLSEIGDLLLFPVKTSIMQHSLSIVNVDTRIVLSELKQ
                     KAGLPGCCLLVRNKMLGLI"
BASE COUNT          881 a          844 c          813 g          969 t
ORIGIN      
        1 ctttcagggc ctcttccttc agattgaggt tatagtacca gatacccagg tcgaggaagg
       61 tttcctccgg catttcatta ttaattaacg aggtaaaata tttccccgcg ccggaagaag
      121 ggtgccagag atatttttca aacctggaaa aatggctaag cggatccatg gcggctattt
      181 tgtccagtat ttgcattgct tctgattccc ggttttgcaa acggtaaatc atagcttcca
      241 accggtaagc gtccaggtta tcctgattac cggtcagact tttagccgca taatccaggg
      301 ccctggcgaa ttcttttttt ctgaaataga tcttgcccag ctccaggtaa gccgggctcc
      361 ggtattccac actcagggtg gcgatgtcaa aaccatcctt cgcatccgct tcatgtccca
      421 gttttaaatt ggttaacccg tagttgaagt tggcggaggg gtcataggta tcaatggaca
      481 aagctcgctt taagctggcc agggcctctg tgtactcgag attcctgtac tgaagcgatc
      541 ccagttcatc cagtgcgggt aaataatttg gatccccggc caggcaggcc ctgagctttg
      601 ctgcggccgc cgggtaggcg cgctcgcgaa tatcctcctt tccctggagg tacaatccgt
      661 acacggaatt ccagtcgaag tgcgccgggg tctccaacgg acggcttaag gtatccgcgt
      721 ctggcgatga ccggtagatg agtttatttt ccccgagggt ggcttcgata ttttctgcgt
      781 tgacatccgt ggaaatggaa tccgcgaagg tctgcagggt attaaactga agttcctttg
      841 aatagatcat tttcccggca gccgttattt tcagctgatc atggatcttt tgcaacggag
      901 aaaaaaagat cttgagccag ccgttttcct ttttgatatt caatgccccg tacggattgg
      961 cctttacaaa acctctggtc tgttttaccg ggaaccagta ttccgtccac cggtctgctg
     1021 catacggcgc aaaacccaga tgtttgaacg gagtgaaact gcttccttcc gcggattggt
     1081 taaacaatct cccgctctgc acttcaacat actgtccgtc cgtatcggag agcaaatgct
     1141 cccaaatcat gccctgttgc gacaatcccc atatccagat tttttttccg gccttatcat
     1201 cgtgggtcga ataccggccc atgccaaagt cgtcgtcgtg ccagtaggct ccgtaaaaat
     1261 cgctgtactt tccgaaaaca tgataggatt tataaccgcc gaaattatta ttctcataaa
     1321 atgaaatatc ccggccattt tgtttgttga cgggccagtc gctatactcc ccttcatggc
     1381 ccagataatg tgtacccgga taaataaact gcaggtttcc ctttgctttg atacccgtat
     1441 tcatccagga gtaataaggc tgttccaggg gcgtgccgtt ataccagaaa gaagtggttg
     1501 taaaataggc tttgtccgcg ggaagattaa tctccagccg ccatttcgag ccggttaaca
     1561 aatccagtac gccaatcaca caacttacgc tgctgtcggg ccgggtaaat gtgctgtagt
     1621 ccaccggtgt ggcacaattc ggcgtatgtc cgataatgcc gtaatttgct tcaatcccac
     1681 cgctcgtcca ggggccgcgc atcgccacgt cccggaattt gaccacgtgg ttattataga
     1741 taaaagactt gccggtggac ttttcaatgg cgctccagat cttaccgccg acttcgggaa
     1801 gtatcgtaat ttttaaataa gcattttcaa gttctaccac cttccacttt ttttgaaccg
     1861 gtttatcggt aaatccatcg tagcggaaat acggatatat gatggcggcg ggattgggaa
     1921 tggggtcggg atcggaaaag ggatagctgg taaacgtttt ttcgtattcc cggaggaagg
     1981 cgcttttttg ggcgcgcagg accccgattg cggagagcag caggaatccg gttatgaaca
     2041 ttctttttct tctgaaaaaa acaaaccggg tttcaaaatc gggccggctg gccgcaataa
     2101 gggtttgggg catttgttgg ttttacagtt taaattaatc ccagcatttt attccggacg
     2161 agcaggcagc aaccgggcaa acccgctttt tgtttcagtt cagaaagaac aatccgggta
     2221 tcgacgttta caatactcag cgagtgttgc atgatggagg tcttaaccgg aaaaagcagc
     2281 aggtccccga tctcggatag taagccgccc aaaacaataa ggtcggggtt cagcatatta
     2341 atcgcgacag ccagtccctt accgaggtta cgcgcaattt ccgttacccc ttcaatcgcc
     2401 agactatctc catgcaacac cgcttccagt atattctcga gttggaggta tccctttttg
     2461 gccagggttt tctggagccg gctgctgctg cctttcatca ttttcgaatt tatgaaatca
     2521 atgagcgcca gtccggatgc ttccgtttcc aaacaacctt ttttcccgca aaagcagatt
     2581 ttttcattgt tgtacatcgg gatatgcccg atttcgcctg cataaccaga ggttccatac
     2641 accggccggc catccacgaa aatacccagc gacattccgt aatcgaggtt tacgatacaa
     2701 acattttttt ccgggtaaga aagaccgtcc cggccaaaat aatattcgcc gtaggcaatg
     2761 gtccggctgt cattatctat gtaaaccgga aggcccagca cctcttccag cctggatttg
     2821 atcggagctt cggcaaagtg ataattactc agtatctggc tggtttttac gttaatgcgg
     2881 cccgacaggc taagcccgag accaataatt ttatctttcg gaataatgga tttggcaaga
     2941 aaggcctgga tttcattgac gatcgcattt aaggattcgt ccggatcgga aaataagaaa
     3001 ggaatgccga gggccgtttc gaccattccc gcattgaagc tcatcagccc gatattgatc
     3061 tcgtatttcc tgatttcaat cccgagaaaa taacagctga ccggattaag cgtgtaaata
     3121 gcggcctttc tcccaagtcc ttccgttttt cgtccggtcg cctttaccaa cccttcactg
     3181 gccaaaccgg agatcagctc cgtggtttta ggcacgctga tatttaactg gtctgccagg
     3241 tccagcatgg tggattcccc tttttcagtc aggaaggcca ccacattctt tttcaggctg
     3301 aggtccttta aagcaacatg ggaaagggaa tggccattta aatgtctaaa aatggtatat
     3361 ggcaatctta agggttttaa agctggaatt attaaaaaaa attaataaat aaaggtatta
     3421 attaaaatat ttattaaaga cgaaagtact tagatatatt tttttataac aaaaataaat
     3481 aacaaatttg taacggacag gattctt
//
LOCUS       DASYCU010000434         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2558459, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000434 DASYCU010000000
VERSION     DASYCU010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558459"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VGM24_08750"
     CDS             complement(<1..630)
                     /locus_tag="VGM24_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4155504.1"
                     /translation="MISSTNGEQKMNGQAEVNRLIDHLFRQESGKIVSVLTRIFGSEN
                     LELAEDVVQDSLVEAIRQWDEKGIPDNPVGWLFRVAKNKALRIVNREKYKRRYVSEVV
                     HLLQSEWTARPAADHLFSENEILDDQLRMMFTCCHPSVSPDSQVALILKTLCGFSISE
                     IASAFLGTEENISKRLVRARQKIRENRVLFEVPAGGDLKARLQTVMETIY"
     gene            complement(686..1243)
                     /locus_tag="VGM24_08755"
     CDS             complement(686..1243)
                     /locus_tag="VGM24_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY4155505.1"
                     /translation="MRKLIYGINLTIDGCFDHDKMMASEAVHEYFTKLVRDAGLLLYG
                     RITYELMVPFWPEIAREQSMSKELNEFALVFDAVDKVVFSKTLKSAEGNTRIVRTDLK
                     DEILRLKQEEGGDILAGGVSIPSQLIALGLVDEFYFVVHPVIAGSGRRLMDGISLSEN
                     LELELVDSKVLKPGWVANHYRTAAR"
     gene            1390..>1763
                     /locus_tag="VGM24_08760"
     CDS             1390..>1763
                     /locus_tag="VGM24_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4155506.1"
                     /translation="MTLSGNTRGQSAVSGQYADIQDLHMYYELSGSGKPLVLIHGGGS
                     TIESSFGRILPTLAKKYRIIAVELQAHGRTKDTGHPLSFERDADDVAGLLEHLNIKTA
                     AIFGFSNGATTALQMAIRHPQQA"
BASE COUNT          416 a          478 c          418 g          451 t
ORIGIN      
        1 gtagatggtc tccattaccg tttgcagtct tgcctttaaa tccccgccgg ccggaacctc
       61 aaacaaaacg cggttctccc ggatcttttg cctcgccctc accaggcgct tgctgatatt
      121 ctcctccgtg ccgagaaagg cgctggcaat ttcggaaatg ctgaacccgc aaagcgtttt
      181 taatatcagc gccacctgtg aatcgggaga aacggatgga tggcagcagg tgaacatcat
      241 cctcagctga tcatccagga tttcattttc ggaaaagaga tgatccgccg ccggccgggc
      301 cgtccattcg gattgcagta gatgaacgac ctcggacaca taccgtcttt tatatttttc
      361 ccggttgacg atcctcagtg ctttattttt agcaacccgg aataaccagc caaccggatt
      421 atcgggaatt cccttttcgt cccattgtct tatcgcttcc accagggaat cctgcaccac
      481 gtcttctgcc agctccaggt tttccgaacc gaaaatccgg gtcagaacgg aaacgatctt
      541 tcccgattcc tgccggaaga ggtgatcgat cagccggttt acttccgcct gtccgttcat
      601 tttctgttct ccgttagtgg atgaaatcat aatggaagca ttgtggtccg gatagatccg
      661 ggttataaat gctataagat tttatctacc gcgctgcggt ccggtaatga ttcgcgaccc
      721 agcctggttt aaggaccttc gaatcgacca gttccaattc taaattctcc gacaggctga
      781 tcccatccat aagccgcctt ccacttcctg cgatcaccgg gtgaacgaca aaataaaatt
      841 catccaccag cccgagcgcg ataagctgcg aaggaatgct tactccgccg gctaaaatat
      901 ccccgccctc ttcctgcttc agccgcagga tttcgtcttt caggtccgta cgaacaattc
      961 tcgtattccc ttccgcgctt tttagtgttt tggagaaaac gactttatcg actgcatcaa
     1021 atactaaagc gaattcattc aattccttcg acatggattg ttcccgggcg atctccggcc
     1081 agaaaggaac catcagctcg taggttatac ggccgtagag gagcagaccg gcatcccgca
     1141 ccagcttcgt aaagtattca tgtaccgctt cgctggccat cattttgtcg tgatcgaagc
     1201 agccgtcgat ggtcaggttg atcccgtaga ttagttttct catttgatca tttttagttt
     1261 accgggcagg cgcccgcttt aaaattagat aataatcagg aaaagatttt tggggccgcg
     1321 ggcttgcatt tatatttgca accgttcagt tgcgtaagga ataaactggt caccttaatt
     1381 attcttatta tgacactatc cggcaatacc cgcggccagt ccgcggtttc agggcagtat
     1441 gcagacatcc aggacctgca catgtattac gaactttccg gttcgggaaa accgctggtg
     1501 ctgatccatg gcggagggtc cacgatcgag agcagtttcg gccggatatt accgaccctg
     1561 gcaaaaaaat accggatcat tgcggtggaa ctgcaggcgc atggccgcac aaaggatacc
     1621 ggccacccct taagcttcga gcgggatgcg gatgatgtgg ccggactgct ggaacacctg
     1681 aatataaaaa cggcagccat ctttggtttc agcaatggag ccaccacggc tttgcagatg
     1741 gcgatacggc atccgcagca ggc
//
LOCUS       DASYCU010000435         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3719481, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000435 DASYCU010000000
VERSION     DASYCU010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3719481"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..886
                     /locus_tag="VGM24_08765"
     CDS             149..886
                     /locus_tag="VGM24_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEY4155507.1"
                     /translation="MEVRIYENYDDMSKAVADLILRQVRQKPDSLLCFPSGDSPTGVL
                     KYLAGYSAAGQIDLATCYFAGLDEWVGMDGSKEGSCRHYLDRNLFHPLGIKPERILFF
                     DGLAADLDRECARVDEWLKEKGPIDLMLVGLGMNGHIGLNEPGTDFSVYSHHSVLQQI
                     TKKVAQKYFKQETSLQEGITLGLRHFAESGTPVLIAGGLKKAAIIAQSLEGGITTQVP
                     ASIVQSLPNGYVFLDQEAASLLNRKAG"
     gene            complement(894..1862)
                     /locus_tag="VGM24_08770"
     CDS             complement(894..1862)
                     /locus_tag="VGM24_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY4155508.1"
                     /translation="MKSLLTGLFCLIGLVSSVLAQHPQLRIEGTNGKFYLVHTIAAKD
                     NWYSIGRLYNASPHDIASYNNLSFDKPLEIGQLINIPLNATNFDQKQKKNYGETLIPV
                     YHLVQEKEWAFRLSSIYNDVPVTSLEKWNKIKRDDVKQGMLLVVGFLKVKTDQSPLAS
                     EAPAVPEQPGSVAQAHTEKPAETKIVDQPAQASSNQKTLTTSTVSTTSTGNTERPGSY
                     AATHAAGGFFSVNFIGNNNKSVSGQAGTFKSTSGWTDGKYYALMNGVAVGTIVRISSN
                     GKSVYAKVLGQLPDMKESNGLVTRISNAAAAELGTGENRFPVDVKY"
     gene            complement(1950..>2442)
                     /locus_tag="VGM24_08775"
     CDS             complement(1950..>2442)
                     /locus_tag="VGM24_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155509.1"
                     /translation="GFFLAVLRKKDGTEWISKGKDRREMPNKNETARLRKFIPDESLQ
                     FIRLQEWTHVMPAHLLSDLLVLKNYLYLKKAGVMAGKMGSEEWIPDHELALCTGLNPQ
                     TPELELSGSDALKYLRREALNPDVQMKGWLPVSYQRQKLGWVKMLANRLNNYYPKSWR
                     ILK"
BASE COUNT          588 a          566 c          599 g          689 t
ORIGIN      
        1 ccgtgtaagc gggtttaaat ttccatccaa aggattcaaa gataaatcgt ccgggttgac
       61 gatctttcca ttttattttc ctgcaggtta tgtgataatt tttatcttac gggttgaacc
      121 cggcttatat tatcttaaaa agagatccat ggaagtacgg atttatgaga attatgatga
      181 catgtccaag gcagtggccg accttatttt gcggcaggtc aggcaaaagc cggattccct
      241 gttatgtttt ccttccggcg attcgcccac gggagtcctg aaatatctgg caggttattc
      301 ggctgccggt cagattgatc tggctacctg ttattttgcc ggactggatg aatgggtcgg
      361 tatggatggg agcaaggaag gaagctgcag gcattacctc gatagaaacc tgtttcatcc
      421 actgggaata aaaccggaaa ggattttatt ttttgatggc ctggcagctg atctggacag
      481 ggaatgcgca agagtggatg aatggcttaa agagaaaggt cctatagatt tgatgctggt
      541 cggactaggt atgaacggcc atatcgggtt aaatgaaccc ggtacggatt ttagtgtgta
      601 ttcccatcac tccgttttgc agcagataac aaagaaagtg gcccagaaat attttaagca
      661 ggagaccagc ctgcaggaag gcattaccct ggggctccgg cattttgccg aatcaggaac
      721 accggttctg atcgccggag gattaaagaa agcagctatc atagcgcaat ccctggaagg
      781 cgggataacg acccaggttc cggcctctat tgtgcagtcc cttccgaacg gatatgtttt
      841 ccttgatcag gaagcggcct cgttgttgaa ccgtaaagct ggctgaggat ctttcaatat
      901 ttcacgtcta ccggaaaccg gttttccccg gtgcccagct cggcagcagc tgcgttgctg
      961 atccgggtga ccagtccgtt gctttctttc atatccggaa gctggccgag gactttggcg
     1021 taaaccgatt ttccattgct gctgatccgc acgatggttc caactgctac cccattcatg
     1081 agtgcataat actttccgtc cgtccacccg ctggtgcttt taaaagtccc tgcctgtccg
     1141 ctgaccgatt tattattgtt tccaatgaaa ttcacactga agaatccgcc cgccgcgtgc
     1201 gtggccgcat aactgcccgg ccgctcggta ttgccggtgg aagtggtgga aacggttgag
     1261 gtggtaaggg tcttctgatt tgaagaagct tgtgcgggct gatcaacaat tttagtttct
     1321 gccggtttct ccgtatgcgc ctgggcaacg gaaccgggct gctccggaac agccggtgct
     1381 tcggatgcca gaggcgactg atccgttttt actttcagaa acccgaccac cagtaacata
     1441 ccctgtttaa catcgtcgcg cttgatttta ttccattttt ccaggctggt taccggaaca
     1501 tcgttgtata tggagctcag acggaaagcc cattcttttt cctgaaccag gtgataaacg
     1561 ggaattagcg tttcgccata attttttttc tgcttctgat caaaattggt tgcgttgagg
     1621 ggaatattga tcagctgacc gatctcgagg ggtttgtcga aactcagatt attgtaggag
     1681 gcaatatcat gggggctggc attgtataaa cgcccgatac tataccaatt gtcctttgcg
     1741 gctatcgtat gaaccaggta aaattttcca ttggtgcctt caatccgaag ttgtggatgc
     1801 tgggctaaga cactggatac cagtccaatc agacaaaata aaccggtgag caggctcttc
     1861 atgagtcata tttttggata aacgtaaaga tcccgaataa tcttgcaaat aaaatattaa
     1921 aaacgggtga aaacgttccg ttatctactt cattttaata ttctccacga cttggggtaa
     1981 taattattta ggcggttggc aagcatttta acccaaccca gtttctgccg ctggtaactg
     2041 acgggtaacc atcctttcat ttgcacatcc ggattaagcg cttcccggcg taaatatttc
     2101 agggcatcgg aaccggataa ttccagttcc ggagtttggg ggtttaagcc ggtgcacaag
     2161 gcgagttcgt gatccggaat ccattcttcc gaacccattt ttcctgccat tacaccggct
     2221 ttcttcagat aaagatagtt tttcaggacc aataaatcac tcaataaatg cgccggcatc
     2281 acgtgcgtcc attcctgaag gcggatgaac tgcagacttt catccgggat gaatttccgt
     2341 aagcgggccg tttcgttttt gtttggcatt tcccgcctgt ccttaccctt cgaaatccat
     2401 tctgttccgt ccttttttcg cagaaccgcc aggaaaaaac cc
//
LOCUS       DASYCU010000436         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1107983, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000436 DASYCU010000000
VERSION     DASYCU010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107983"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VGM24_08780"
     CDS             <1..443
                     /locus_tag="VGM24_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY4155510.1"
                     /translation="PSGTSGEERQGFKYKWLDEFFIYISEIKKAHPKLILCGDYNIAH
                     KEIDIHDPKGNKNSSGFLPAERAWMDSFLKSGWTDSFRKLHPEPHRYSWWSQRFPTVR
                     LNNKGWRIDYINVSDNLAGNILKSDIYPDVKQSDHCPVYLELKR"
     gene            784..1053
                     /locus_tag="VGM24_08785"
     CDS             784..1053
                     /locus_tag="VGM24_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY4155511.1"
                     /translation="MNKAELIAKIADDAGITKTQANESLDSFVEAVAKTLKGGGKVTL
                     VGFGTFSVSKRAARNGRNPQTGAVIKIKAKKVAKFKAGKELSAKL"
     gene            1151..1255
                     /locus_tag="VGM24_08790"
     CDS             1151..1255
                     /locus_tag="VGM24_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HEY4155512.1"
                     /translation="MGKGDKKTKKGKTFMGSFGKSRPKNPKKTAKKKP"
     gene            complement(1294..1935)
                     /gene="pdxH"
                     /locus_tag="VGM24_08795"
     CDS             complement(1294..1935)
                     /gene="pdxH"
                     /locus_tag="VGM24_08795"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY4155513.1"
                     /translation="MSSIADIRKEYRLKNLNEAEAHQNAIQQFDLWWNEALSSEIDEV
                     NAMTLATASSEGLPAARIVLLKGYHARGFQFFSNYHSAKGLQLQENPRACLVFFWKEL
                     ERQVRITGLIEKLSEKESDEYFESRPAGSRIGALASPQSEVIESSQWLNDRASQLEKE
                     YAHSDIPRPPHWGGYLVRPVTIEFWQGRPNRLHDRLLYTLQGDGNWVIDRLAP"
     gene            complement(2067..>2821)
                     /locus_tag="VGM24_08800"
     CDS             complement(2067..>2821)
                     /locus_tag="VGM24_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155514.1"
                     /translation="METEDLHEQLKELEEKYLKTQALVKPPTSSEDYLSQLKFAQDNL
                     AQHNQHINRLLQQLQVLKESEKKYQDLQHHQVLLNEQISSLQRQLSEKDEEIHRIMQE
                     QRLVLEMNERMDKAFSDFTLMQDKLRKLEGYLDQPHHKRIEYEQLQESYFKISREYDE
                     VKGKQMALFDENQRLSRILADTEDKLKESNFQRLQYQKKMLFLEELNRDLQEASEQHK
                     KMESQLRRISEMEAILSRAYPEKKEPPEQPII"
BASE COUNT          720 a          685 c          667 g          749 t
ORIGIN      
        1 tcccttccgg cacctccgga gaggagcgcc agggatttaa atacaaatgg ctggacgagt
       61 ttttcattta tatatcggag atcaaaaaag cacatcccaa attaattctt tgcggcgatt
      121 acaatattgc ccataaggaa attgatatcc atgatccgaa agggaataaa aattcttccg
      181 gttttctgcc ggcggaaaga gcctggatgg attcttttct gaaaagcgga tggacagaca
      241 gctttcgcaa acttcacccg gagccgcatc gctacagctg gtggagtcag cgtttcccaa
      301 cggtccggct gaacaacaag ggctggagaa ttgattatat caacgtgtcc gacaacctcg
      361 ccggcaacat tttgaaatcg gatatttatc cggatgtaaa acagagcgat cactgccccg
      421 tttacctgga gttgaaaaga taatcttctg ccgtcacgat aaaacagtcc ggccataccc
      481 cgcttgaaca ccggatcgtc ttgtgcaata cttgtggaaa agaagtaaga ggaagatcct
      541 ccttccgcga aaacaaacgc gtccctctgg aatcctttat acgcaagggt tttggataag
      601 aagacagaac ctttgccagg caagggtttc agcgcccgga acaaaaatac ccggtgaacg
      661 ccataccgta attttcccct gaaaccctta tccgtcctga actttatcaa ttttttatct
      721 cttcaaaaaa ttttgcctga tttaaaaagc aggtatattt gttccgtata aaaaatttac
      781 actatgaaca aagctgaatt aattgcaaag attgcagacg atgcaggtat tacaaaaacg
      841 caggcgaacg aatccctgga ttcatttgtg gaagcagtgg caaagacgtt gaaaggcggt
      901 ggtaaggtta ccctggtggg atttggaaca ttttcagttt ccaaaagagc tgcccggaat
      961 ggtcgcaacc cacagaccgg cgcggtaata aaaatcaaag cgaaaaaagt agcaaagttt
     1021 aaagcaggta aggaattgtc tgctaaatta taatgctgca cgtatttact gcattttaaa
     1081 gctctggagc agataaaaaa tcgccggagc ttttgttttt tccggatatt tgcgattata
     1141 aaatcacatc atgggtaaag gagataaaaa aacgaaaaag ggtaaaacat ttatgggctc
     1201 attcggaaag agccggccga agaacccgaa gaaaacggct aagaagaaac cgtagcggga
     1261 tcaattatcc ggactgccgg cggccgagga ttcttacgga gccagccggt cgataaccca
     1321 gttcccatct ccctggagcg tatacaaaag ccggtcgtgc agccggtttg gcctgccctg
     1381 ccagaattct atagtgaccg gtcttaccag atatccaccc caatgcggcg ggcggggaat
     1441 atcggagtgg gcatattctt tttccagctg ggaagcccgg tcattcaacc actgactgct
     1501 ttcaattact tcgctttgcg gggaagccag cgcaccaatg cggcttccgg ccgggcggct
     1561 ttcgaaatat tcatcactct ccttttcgct cagcttttca atcagtcccg tgatccgcac
     1621 ctggcgctcc agctctttcc agaaaaaaac caggcatgct ctcgggtttt cctgcaattg
     1681 caggcctttg gcactatggt aattgctgaa gaactgaaat cccctggcat ggtaaccctt
     1741 gagtaagaca atgcgggcgg cgggaagccc ttctgaagaa gccgtggcca gcgtcatcgc
     1801 attcacttca tcgatctcgc tgctcagcgc ttcattccac catagatcaa actgttggat
     1861 cgcattttga tgcgcctctg cttcgttcag gttctttaac ctgtattctt tccggatatc
     1921 ggcgatggaa ctcatgctgc aaaattacat ttttaccccg aaccggaaca gggatcgctt
     1981 aaggcagaaa acgctttcta aatctttgaa ggcgaccccg gcttaaacga gggcggtaaa
     2041 tgtgacccgc attccgtgcg gtccgctcag atgatcggct gctccggagg ttctttcttc
     2101 tcggggtaag cacgcgaaag gatagcttcc atctcgctga tgcggcgcaa ctggctctcc
     2161 atttttttgt gttgttcgga ggcctcctgc aggtcccggt tcaattcttc caggaacaac
     2221 attttcttct ggtattgcag gcgctggaaa ttcgactctt taagtttatc ctccgtatcg
     2281 gccaggatgc ggctcaggcg ctgattttca tcaaagaggg ccatttgttt ccccttcact
     2341 tcatcgtatt cccggctgat cttaaaataa gattcctgca attgctcgta ttcgatccgc
     2401 ttgtgatgcg gctgatccag gtaaccttcc agctttcgca gcttgtcctg catgagggta
     2461 aaatccgaaa aggccttatc catccgctca ttcatttcca gtacaagccg ttgctcctgc
     2521 atgatgcggt ggatctcttc atctttttcg gaaagctggc gctggagaga agatatctgt
     2581 tcattcagca ggacctggtg atgctgaaga tcctggtatt ttttttctga ttccttcagc
     2641 acctgtaatt gctgcaggag ccggttgata tgttgattat gctgtgcgag attgtcctgg
     2701 gcaaatttca gctggctcag gtaatcttcc gaagaggtgg gcggcttaac gagcgcctgc
     2761 gttttcaaat atttttcctc gagctctttc agctgttcgt gaaggtcctc cgtttccatt
     2821 c
//
LOCUS       DASYCU010000437         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5020747, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000437 DASYCU010000000
VERSION     DASYCU010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5020747"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1081)
                     /locus_tag="VGM24_08805"
     CDS             complement(116..1081)
                     /locus_tag="VGM24_08805"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY4155515.1"
                     /translation="MVYIISQHILPMQVYRNTDSLPDFHNAVVTIGTFDGVHSGHQQV
                     ITQMKQEAARILGESVIITFDPHPRTVVDINSGIRLINTTEEKTELLSGKGIDNLVII
                     PFTEKFARLSAEDYISRFLVRYFHPHTIIIGYDHHFGKGRTGNYQLLNKMAGTYHYQL
                     KEIPAHVLDSISVSSTRVREAISLGDMETANTLLGYTYFFSGTVQEGNKLGRSIGFPT
                     ANLQVADSFKLLPGDGVYAVDVILEDEPERRFKAMMNIGYRPTVDGSRKVIEVNIFDF
                     DRMIYGRTVKVFVRKFLRPEQRFSGLEELKAQLGEDRRQAEQIEN"
     gene            1170..2342
                     /locus_tag="VGM24_08810"
     CDS             1170..2342
                     /locus_tag="VGM24_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY4155516.1"
                     /translation="MSLYSRRGVSAQKEEVHQATASLDKGLYPFAFCRIYPDFLGKDK
                     DWVNLMHADGAGTKSILAYLFWKETGDLSVWRDIAQDAVAMNLDDLLCVGIYDNLLFS
                     STIDRNKKRIPGEVLEAIIRGTQEFFDELKGYGVNIQYLGGETADVGDLVRTVAVNGT
                     MTARWTKSGIISNEKIAAGDVIIGLSSSGQARYEKKYNSGIGSNGLTSARHDVLTELY
                     GTAHPESFDPDLEKDLVYTGSHKLTDEIKTERGETLSVGRLLLSPTRTYAPVAKALLE
                     EHFDQIHGMIHSSGGGQTKCLKYLPRPLKIIKDHLFEPPVVFQMIRESSGADDREMYQ
                     VFNMGQRLEIYTDPASATGIIDRIRSFGIEARVIGRVEAAAKKELLIDTGTNQIGY"
     gene            complement(2392..2550)
                     /locus_tag="VGM24_08815"
     CDS             complement(2392..2550)
                     /locus_tag="VGM24_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155517.1"
                     /translation="MENIHVLSGPEALKAESIRVIRESGKWTPAKDHGKIVKSYHKQP
                     IIYRLEPA"
BASE COUNT          592 a          663 c          683 g          618 t
ORIGIN      
        1 aataccctgc tcctgctgca tcaatacaat ctgaaaagta tcggggtgaa taatccggca
       61 gtggacgacg cctcgctgat gaaggaactg attgcgaaga tacttctgta gatttctaat
      121 tttcaatttg ctctgcctgc cggcggtcct ctcccagctg ggccttcagc tcttccagtc
      181 cgctgaaccg ctgctcgggg cgtaagaatt tcctgacgaa taccttgacg gttcttccat
      241 agatcatccg gtcgaaatca aaaatattca cttcaatcac tttcctgctt ccatctaccg
      301 ttgggcggta accgatattc atcattgcct taaaccggcg ttccggttca tcctccagga
      361 tcacgtcaac ggcgtaaacc ccgtctcccg gcaatagttt aaaggagtcg gccacctgca
      421 ggttggcggt gggaaaaccg attgaacgtc ccagtttatt tccctcctga acggttccgc
      481 tgaaaaagta ggtatagcct aataaggtat tggcggtttc catatctccc agcgagatgg
      541 cttcccgcac ccgcgtggag ctcacggaaa tggaatccag cacatgcgcc gggatctcct
      601 tcaactggta atgataggtt ccggccatct tgttaagcaa ttgataattt cccgttctgc
      661 cctttccgaa gtgatggtca tagccgatga taatggtgtg ggggtgaaag tatctgacca
      721 ggaaccggct gatataatct tccgcagaca gccgggcgaa tttttccgta aacggaatga
      781 tcacgaggtt atcgatccct ttgccggaaa gcaattctgt tttctcttcg gtcgtattga
      841 tgagccggat gccggaattt atatcgacaa cggtcctggg atgcggatcg aacgtaatga
      901 tcacggattc tccgaggata cgggcagcct cctgcttcat ctgggttatc acctgctggt
      961 ggccgctgtg cacgccgtca aaagttccga tggtgactac ggcgttgtgg aaatcgggta
     1021 aagaatctgt gttccggtag acctgcatgg gtaaaatgtg ctgcgaaatt atataaacca
     1081 tccctcacgg cttaaaattt ttcgtttttc gtttaccggc ttggtccttt gggttagctt
     1141 tgccgcttat caaatctccg tttttcagta tgtccctcta ttcccggcgc ggtgtttctg
     1201 cccagaaaga agaagttcat caggcaacgg catcgctgga caaaggcctt tatccgtttg
     1261 ctttttgcag gatctatccc gattttctgg gaaaggataa ggactgggtg aacctgatgc
     1321 acgcggatgg cgccggcacc aaaagtattc tcgcctattt gttctggaag gaaaccggcg
     1381 atctctccgt ttggcgcgac attgcgcagg atgccgtggc gatgaacctg gacgacctgc
     1441 tctgcgtggg catatatgac aacctgcttt tttcgtccac catcgaccgg aataaaaaaa
     1501 ggatccccgg cgaagtgctg gaagcgatca tccggggtac gcaggaattt tttgatgaac
     1561 tgaaaggtta cggggtaaat atccagtacc tcggcggtga aacggcggac gtgggcgatc
     1621 tggtaagaac ggttgccgtg aacggtacga tgacggcccg ctggacaaaa tcgggcatca
     1681 tcagcaatga aaaaattgca gcgggagatg tgatcatcgg cctctccagc agcggacagg
     1741 cccggtatga aaaaaaatac aatagcggaa tcggcagcaa cggcctgacc agcgcccgcc
     1801 atgatgtgct cacggaatta tatggaaccg ctcacccgga atcttttgat ccggacctgg
     1861 agaaggacct ggtttatacc ggctcccaca agctgaccga tgaaataaaa accgaacggg
     1921 gagagactct gtccgtcggc cgcctgctcc tgagccctac gcgcacctat gctccggtgg
     1981 caaaggctct gctggaagag cattttgacc agatccatgg aatgatacac agcagcgggg
     2041 gcgggcagac caaatgcctg aaatacctgc cgcggccgct gaagatcatc aaggatcatc
     2101 tgtttgagcc gccggtagtc tttcagatga tccgggaatc ttcgggggca gatgaccggg
     2161 agatgtacca ggtgttcaat atgggccagc gcctggaaat atataccgat cccgcctccg
     2221 caaccggaat catcgaccgg atccggtcct ttggcattga ggcccgcgtg atcggccggg
     2281 tggaggcggc cgcaaaaaaa gagttgctga tcgatacggg aacgaatcag atcgggtact
     2341 gaaccggtcg gagaacgcgc ggaccaattc cattctacgg aaacctccgg tctacgcagg
     2401 ctccagccgg tagataatgg gctgcttgtg gtaggacttg actattttcc cgtgatcctt
     2461 tgccggcgtc cattttccgc tttcccggat cacccggatg gactctgctt tcagggcttc
     2521 cggtccgctg agcacatgaa tattctccag cgttcc
//
LOCUS       DASYCU010000438         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1253695, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000438 DASYCU010000000
VERSION     DASYCU010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253695"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VGM24_08820"
     CDS             complement(<1..695)
                     /locus_tag="VGM24_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HEY4155518.1"
                     /translation="MKRRIRHLGLVVFILASLIKVNAQSNDSSRYFYLKALDEKSGRL
                     FLAAYNDFRQSLEYDSTNAETLREAGLTAVELRKYENAKLAFEKLIALKKNDTTAVNQ
                     LAILNFWTHQWEKACFYASRALQLHTGKDNYYILGKSYYELEDYGHAFGYLPVAAKED
                     PRNAEIPYLIARAYVDMNNYKPAIPFFQKAIALDSSKVEWIYECALVFATLYNDKSAI
                     EYYEIAAAKGYKK"
     gene            complement(743..2686)
                     /gene="glgB"
                     /locus_tag="VGM24_08825"
     CDS             complement(743..2686)
                     /gene="glgB"
                     /locus_tag="VGM24_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEY4155519.1"
                     /translation="MITSYEHTHFVDSTKPVWNYSLFSDSDISNFQNGTHYRLYEVFG
                     SHACIVLGKSGYYFAVWAPNATYVSVIGNFNDWNPAAHPLFVRLEKSGIWEGFIPDLP
                     RGEVYKYHIRGFNQKETVKGDPFANFWEKRPNTASITWDLGFDWKDQEWMETRGEHNG
                     LNKPWSVYEVHLASWMRPDKNDEETYNSYDQIRETLVPYVKEMGFTHVELMPVMEFPF
                     DGSWGYQGAGYFAATSRFGDPQSFMTLVDAFHAAGIAVLLDWVPSHFPYDAHGLFMFD
                     GTNTYEYADMRKGFHPDWNSYIFNYKRGEVKSFLISSGRFWCDRFHADGLRVDAVSSM
                     LKLNYSREKGQWEPNEHGGDGNLEAIAFIKDLNETLFRDFPDIQTIAEEATDWPGISK
                     PTFAGGLGFGMKWMMGWMHDTLDYFKADPLFRKDLQNKFTFSMMYFYDENFMLPLSHD
                     EVVHGKSPMLYKMPGDDWQKFANLRLMYAYMFTHPGGKLLFMGNEFGATAEWNYKSEL
                     QWELLQYDSHRLLKDCLRDLNLLLRREPALYENQFNPSGFEWIDLDHRNESVIVFRRK
                     GELPENDLLIILNMTPAVRTNWKIYANGKSAWKEIFNSDQKKYWGTGDTLNPDPMVTL
                     VDKNQNRYEINIHLPPLGAVVLK"
     gene            complement(2716..>3806)
                     /locus_tag="VGM24_08830"
     CDS             complement(2716..>3806)
                     /locus_tag="VGM24_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY4155520.1"
                     /translation="INQWPSYQNTNHREEAMFDPHGSAKDKHIMEAGWFVPHLPDLNL
                     KNKWLAVFLIQNAIWMTETYQIDGWRVDTYKYCDPHFLNRLNAALAAEFPSLTSFGEA
                     WVNSVPASAYFCRNNMDVPFKHNLLGVTDFPVSFAMLESLRKNYGWTDGVNRLYTVLA
                     LDMLYQDPMHNCIFFDNHDMNRFYSEVGEDMASFKMGMGLLLTMRGIPEIYYGTEILM
                     KNFKNPSDAMVREDFPGGFPGDSVNKFEPSGRTEPENAAFDYISELAAFRKTSSALGS
                     GALMQYIPVNGLYVYFRYDKKQTIMCVLNTDTLDRKVDFSDYSERTKGFAGATELLSH
                     TNLDIPGKMDIPPRNMFILELKKQAGKQ"
BASE COUNT          953 a          931 c          874 g         1048 t
ORIGIN      
        1 tcctttttat atcctttagc agctgcaatt tcatagtatt cgatggctga tttgtcgttg
       61 tagagggtgg caaacacaag ggcgcattca taaatccact ccactttcga tgaatcgagg
      121 gcaatggctt tctggaaaaa aggaatcgcc ggcttgtaat tattcatatc gacgtaagcc
      181 cgggcaatca ggtaaggaat ttccgcattg cgcggatctt cctttgcggc caccggtaaa
      241 taaccgaatg catgtccata gtcctccagt tcataataac tcttacccag gatataataa
      301 ttatcctttc ccgtatgcag ctgaagggca cgcgaggcgt agaaacaggc tttttcccac
      361 tgatgcgtcc agaaatttaa aatggccagc tgattgacgg ccgtggtatc atttttctta
      421 agagcgatta atttttcgaa tgccagtttt gcattttcat atttcctcaa ttcaacagcg
      481 gtgagaccgg cctcccgtaa tgtttccgcg ttggtggagt cgtattccag ggattgccgg
      541 aaatcgttat aagcggcaag gaaaaggcgg cccgattttt catcaagggc cttcagataa
      601 aaataacggg agctatcatt agactgggca ttgaccttga ttaaagaggc caggataaat
      661 acgacaagac caagatgtct tatgcggcgc ttcattttca tagttgtaaa cccttaatac
      721 caacaaaacc cgggccaatg ggctatttca gaactacggc tcctaacggg ggcaggtgta
      781 tatttatttc gtaccggttt tggtttttat ccaccagggt taccatggga tccggattaa
      841 gggtatctcc tgtaccccag tattttttct gatcactgtt gaagatttcc ttccaagctg
      901 atttaccatt ggcgtaaatc ttccagttcg ttcttacggc cggagtcata ttgagtatga
      961 tgagaaggtc attttcggga agctcgcctt ttcttctgaa aacgatcacc gattcattcc
     1021 ggtgatccag atctatccat tcaaacccgg aagggttaaa ctgattttcg tacaatgccg
     1081 gctccctgcg cagtaataaa ttcaaatcgc gtaaacagtc tttcagcagg cggtgcgaat
     1141 cgtattgaag caattcccat tgcaattcac ttttataatt ccattccgct gtagctccga
     1201 actcattgcc catgaaaagc agttttccgc ccggatgggt aaacatgtac gcatacatca
     1261 gccgcaggtt ggcaaatttc tgccagtcat cgcccggcat cttgtacagc atgggtgact
     1321 tgccatgaac gacttcgtcg tgactcagcg gaagcatgaa gttttcgtca taaaaataca
     1381 tcatgctgaa ggtaaattta ttctgaaggt ccttccggaa cagggggtcg gccttgaaat
     1441 aatccagggt atcatgcatc cagcccatca tccatttcat tccgaatccg agcccgccgg
     1501 cgaaggtcgg cttactgatg ccgggccagt ccgttgcctc ttccgctatg gtctgaatat
     1561 cggggaaatc acgaaacagg gtttcgttca gatctttgat gaaggctatc gcttccagat
     1621 tgccgtcgcc tccgtgttca ttcggttccc actggccttt ttccctcgaa taatttaatt
     1681 tcagcatgga gcttacggca tccacccgga gtccgtctgc gtgaaaccgg tcgcaccaga
     1741 atcttccgct gctgatcaaa aaggatttta cttctcctct cttatagttg aatatatagc
     1801 tgttccaatc cggatgaaaa cctttgcgca tgtctgcata ctcataggta ttcgtgccat
     1861 cgaacataaa taaaccgtgc gcatcatagg gaaaatggga aggaacccaa tccagaagaa
     1921 ccgcgatgcc ggcggcatgg aatgcatcca ccagggtcat aaaagactgc ggatcgccga
     1981 agcgggaagt ggcggcgaaa taaccggcgc cctggtatcc ccagcttccg tcaaaaggga
     2041 attccatcac gggcatcagc tccacgtggg tgaatcccat ttcttttaca tagggaacga
     2101 gcgtttctcg gatctgatcg tagctgttgt aggtttcctc atcgtttttg tcgggtcgca
     2161 tccagctggc gagatgtact tcgtaaacgc tccagggttt gttgagtccg ttatgctccc
     2221 cgcgcgtttc catccattcc tgatccttcc agtcaaatcc aaggtcccag gtaatggaag
     2281 ccgtattggg tcgtttctcc caaaaattgg cgaagggatc tccttttacc gtttcctttt
     2341 ggttgaaccc ccggatatgg tatttgtata cttcccctct tgggagatcc ggtatgaatc
     2401 cttcccagat gccggatttc tcgaggcgga caaataaagg atgcgcagcc gggttccaat
     2461 cgttgaaatt tccaatcacc gaaacatagg tggcattcgg tgcccacacg gcaaaataat
     2521 acccggactt accgagcacg atacaggcat gcgatccgaa tacctcgtac aaacggtaat
     2581 gcgtgccatt ctggaaattg ctgatgtccg aatctgaaaa aagagaataa ttccacaccg
     2641 gttttgtgga atctacgaaa tgggtatgtt cataactcgt tatcacatga tatccctctt
     2701 cttaaatata cgatcttatt gcttgccggc ctgctttttc aattcaagaa taaacatatt
     2761 tcttggtgga atgtccattt ttccgggaat atccagattc gtatgggata gcagttcggt
     2821 ggcgccggca aaacccttgg tcctttctga ataatcgcta aaatcgacct ttctgtccag
     2881 tgtgtcggta ttgagcacac acatgatggt ttgcttttta tcgtagcgga aataaacgta
     2941 taatccgttg accgggatat attgcataag cgctccggat cccagggccg aagacgtttt
     3001 ccggaaagca gccagttccg aaatataatc gaaagccgcg ttttccggtt ccgttctgcc
     3061 ggaaggttca aatttattta cggaatctcc cggaaagccg cccggaaaat cttccctgac
     3121 catcgcatcc gaaggatttt taaaattttt catcaggatt tccgtgccat aatatatttc
     3181 cggaatgccc cgcatggtaa gcagcaggcc catccccatt ttgaaactgg ccatatcttc
     3241 accaacttcc gaataaaagc ggttcatatc gtgattgtca aaaaagatac agttgtgcat
     3301 cgggtcctga tatagcatgt ccagggccag gaccgtataa agccggttca cgccgtctgt
     3361 ccatccgtag tttttccgca gggattccag catcgcaaaa ctcaccggga aatcggtcac
     3421 ccccagtaaa ttatgtttga acggaacgtc catattgttc cggcagaaat aagcgcttgc
     3481 cggaacgcta tttacccagg cttccccaaa agaggtaagg gatggaaatt ctgcggccag
     3541 tgcggcattc agccggttga ggaaatgcgg gtcgcaatat ttataggtat ccacccgcca
     3601 tccgtcaatc tggtatgttt ccgtcatcca gatcgcattc tgaatcagga agacggccag
     3661 ccatttattt tttaaattca ggtccggcag gtggggaaca aaccagccgg cctccatgat
     3721 atgcttgtct ttggctgaac catgcgggtc gaacattgct tcttcccggt gattggtatt
     3781 ctgataggac ggccattggt tgatcc
//
LOCUS       DASYCU010000439         5424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5020963, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000439 DASYCU010000000
VERSION     DASYCU010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5020963"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VGM24_08835"
     CDS             complement(<1..909)
                     /locus_tag="VGM24_08835"
                     /inference="COORDINATES: protein motif:HMM:NF025665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEY4155521.1"
                     /translation="MNIRPIAFYLPQFHPIPENDSWWGKGFTEWTNVAKARPFFKSHY
                     QPQLPADLGFYDLRVPEVRRQQADLARENGIFGFCYYHYWFNGRRILERPFQETLESG
                     EPDFPFMLCWANENWTRIWDGGEHNILLQQHYTEEDDRKHIRELIPVFKDKRYIRIKN
                     KPVFCIYRSSLLPDAAKTLRIWREEARLAGLELYLCRVENFGNQGADFMQAGFDAAIE
                     FQPFMNTLDRFKKEVLGSRLKKDLMGRALLKYYHLTDPSKEEKFLRKRFSNIDFNEYV
                     DFVLKVNRYPADYLRFPGVTPSWDNTA"
     gene            complement(923..1726)
                     /locus_tag="VGM24_08840"
     CDS             complement(923..1726)
                     /locus_tag="VGM24_08840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4155522.1"
                     /translation="MPAAPLFSVIIPTYNSEDFVRTALESILQQSFRDFEILIIDGLS
                     SDQTVKRVRDLNDGRIRILCEKDNGIYDAMNKGIREARGEWLFFLGSDDQLFSDSVLE
                     EVSRNLVNQNVVYGNVKICGDNSWARDGQIYDGPFDLDKLFNKNICHQSVFYRASFID
                     KAAISFNVKYKLCADWDFNLKCRSMTGFFYLDRVIANFNSGGETTLSGTDDEFSRDML
                     SNLMTYFKISSFHPLLANGNFFRYQEVLSRQKKSNPLRYMLRKLSGHLN"
     gene            complement(1726..2601)
                     /locus_tag="VGM24_08845"
     CDS             complement(1726..2601)
                     /locus_tag="VGM24_08845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4155523.1"
                     /translation="MLKEFPKISIITPSFNDASFLEKTILSVIDQEYPNLEYIVIDGG
                     STDGSVDIIKRYEGRIGYWVSEPDKGQYDALQKGFSRSTGEIMGWINSDDMHHPGSLF
                     TIAQIFSDHQQVNWLQGVPNMIDESGRIVYALTAGEIDRYFFYGRKYIQSHRFIQQES
                     TFWRRTLWEKAGNRISTEYKYAGDFDLWIRFFQFEKLYNFHALLGSFRYSRSGQASFE
                     NYQEYIRETLAILEKYPLNKKEKRQMQYLDMFHTIEKNFHKLGSGLKNRLGLKETSVV
                     NDRLNFDYRTQEFKM"
     gene            complement(2594..3325)
                     /locus_tag="VGM24_08850"
     CDS             complement(2594..3325)
                     /locus_tag="VGM24_08850"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY4155524.1"
                     /translation="MSLLKSIIRKAIISAGYSIQKNGVLSGDPASMAAALQRLRQWEI
                     RPEIIIDLGAAQGTWTEKALMVWPDARYELVEPLAEQRNRLENLKSKHSGINYHLAVA
                     GEASGEVFLNVAPDLDGSGVYGTQSGDNVRKVPVITIDEMVKGIEGNILIKFDTHGYE
                     VPILKGTTEALKRTSALIIEVYGFPISPTCLLFQELSSYLDGLGFRLIDMVDIMRRPG
                     DHAFWQADAVYLRKDNPVFQKNSYA"
     gene            complement(3325..4353)
                     /locus_tag="VGM24_08855"
     CDS             complement(3325..4353)
                     /locus_tag="VGM24_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155525.1"
                     /translation="MDFVFTICSNNYLSQAIVLGNSLKLHEPRFCFLIALVDEKDNRI
                     DYDSIPFEVLPVNLIIPDFDELAQKYNIIELNTSVKPGVIEFLFLKRSAGTVIYLDPD
                     IRVFAPFTELEEAFSQYGIVLTPHIYTPIPLDGKIPSENLFLNYGIYNLGFLGLKNGG
                     ETLKFLKWWKERTYSLGYIRISEGLFVDQLPVNLVPLFFNNVGILKCMGYNMAPWNLH
                     ERYLSRVNGEYFVNDREKLRFFHFSSFIIDSGELSVPFYNRFNLKERPDLKEIYEQYN
                     EELKSAGYFFYSGFKCSYMAKKEIVLAQDKLNSWKKKSALRRMAIRAVRLFPDSLKKG
                     LAKFGHLE"
     gene            complement(4362..>5424)
                     /locus_tag="VGM24_08860"
     CDS             complement(4362..>5424)
                     /locus_tag="VGM24_08860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY4155526.1"
                     /translation="GSYQSYKQQPTYQKARGLFAGFHTRVTKEQQDFVNYELGIGNSI
                     SRMKAAGLLYKAFIKFYLIPKFKKGGRSMISKLPGLNTGFKYLKNAALLRLKKDEHAH
                     LLKNKFLSLRELVRLKYTNQEDSCLMGRKMLITDSFWYLHSLKEIFVDEVYRFRSFSE
                     KPYILDCGTNIGLSIIYFKRLFPKARIVAFEPDFSIYNKLIRNLETFEIGDVDVQCKA
                     VWVKEEMLNFAVTGSLGGRLSDHSAETTIQVQATRLRDYINEPVDFLKIDIEGAEFEV
                     LTDCANLLHNVKHLFVEYHSLPDKGQDLDRLLSILNEAGFRVYIKEAWNNMNNPFLHE
                     DYRPTYDLQLNIFGYRVSK"
BASE COUNT         1465 a         1338 c         1130 g         1491 t
ORIGIN      
        1 cgccgtattg tcccaggatg gagtaactcc cgggaaacgt aaatagtcgg cgggataccg
       61 gttgaccttc aaaacgaaat ccacatactc attaaaatcg atattggaaa accgttttct
      121 caggaatttt tcttccttcg acggatcggt caggtgataa tatttaagca gggcccggcc
      181 catcaggtcc ttttttaacc tggatcccaa aacttctttt ttaaaccggt cgagcgtatt
      241 catgaaaggc tgaaattcaa tagcggcatc aaaacccgcc tgcataaaat cagccccctg
      301 gtttccgaaa ttctccacac gacacaggta taattccagg ccagccagcc gggcttcttc
      361 ccgccagatc cggagggtct ttgccgcatc cggcaataag gaagaccggt aaatacagaa
      421 aacaggctta ttctttatgc gtatatatct tttgtcttta aaaacaggaa tcaattccct
      481 tatatgttta cggtcatcct cttccgtata gtgttgctga agcaatatat tatgctctcc
      541 cccgtcccat attcttgtcc agttctcgtt ggcccagcac agcatgaaag gaaaatccgg
      601 ctcaccgctt tccaacgttt cctgaaaggg cctttctaaa atccttcgtc cgttaaacca
      661 gtaatgatag tagcaaaacc caaaaattcc gttttccctg gcgagatcgg cctgttgccg
      721 tctcacttcc ggcacccgta aatcataaaa tcccaggtct gccggcaatt gcggctgata
      781 gtgggattta aaaaaaggcc tggctttcgc gacgtttgtc cattccgtaa acccctttcc
      841 ccaccagctg tcattttctg gtatcggatg aaactgcgga agatagaacg cgatgggtct
      901 tatattcaat cctggaagat ctttaattta agtgaccgga tagttttcgg agcatatatc
      961 tcaggggatt ggattttttt tgccggctca gcacttcctg gtaccggaag aaatttccgt
     1021 tggccagcag cggatggaag gaagaaattt tgaaataggt catcagattg gacagcatat
     1081 ccctggaaaa ctcatcatcc gtaccggaca gcgtggtttc gcccccggaa ttaaaattcg
     1141 caatgaccct gtccaggtaa aaaaaaccgg tcattgaacg gcacttcaga ttaaaatccc
     1201 agtccgcaca gagcttatat tttacattaa aggagatcgc cgccttatct atgaaagaag
     1261 cccggtaaaa aacagactgg tggcagatgt tcttattaaa cagcttatcc aggtcgaacg
     1321 gtccatcata aatctggccg tccctggccc aggaattgtc tccgcatatt ttcacatttc
     1381 catataccac attctgattt acgagattcc gggacacttc ttccagcacg gaatctgaaa
     1441 acaactggtc atccgatcca agaaaaaaca gccattcccc gcgggcctcc cggattccct
     1501 tattcattgc gtcataaatc ccattgtctt tttcacacaa aatccggatt ctgccgtcat
     1561 taagatcccg gacccgcttt acggtctggt ctgacgacaa gccgtcgatg attaagattt
     1621 caaaatcccg gaaggactgt tgaaggatgc tctccagggc ggtccggaca aaatcttcag
     1681 aattataagt aggtatgatt acagaaaaaa ggggagccgc cggcatcaca ttttaaactc
     1741 ctgggttcga taatcaaaat tcaaccggtc attgacaacc gaggtttctt tcaggcccag
     1801 tctgtttttt agcccggaac ccaacttatg aaagttcttc tcaatcgtat ggaacatatc
     1861 caggtattgc atttgccttt tttctttttt attcagcgga tatttttcca gaatggctaa
     1921 ggtttcccgg atatattcct ggtaattctc gaaggaggcc tgtccggacc ggctgtaccg
     1981 gaatgaaccc aacagggcat gaaagttata tagtttctca aattggaaga agcggatcca
     2041 taaatcaaaa tcaccggcat atttgtattc ggtggaaatc cggttaccgg ccttctccca
     2101 taaagtcctt ctccaaaaag tggattcctg ctgtatgaac cggtgcgatt gaatatactt
     2161 ccttccataa aaaaaatacc ggtcaatttc ccccgcggtt aaggcataaa cgatccggcc
     2221 gctttcatca atcatattcg gaacgccctg aagccagttc acttgctgat gatccgagaa
     2281 tatctgggca atggtaaaca aggagcccgg atgatgcata tcgtctgagt ttatccatcc
     2341 cataatttct ccggtggaac ggctaaagcc tttttgaagg gcgtcatact gtcctttatc
     2401 cggctcactg acccaatagc ctatccggcc ttcatagcgt ttaatgatat cgacggaccc
     2461 gtccgtactt ccaccatcga ttacaatata ttccagatta ggatattcct gatcaataac
     2521 ggacaatatg gttttttcca aaaaggaagc atcattaaaa gaaggagtga taatggagat
     2581 ctttggaaac tccttaagca tagctgttct tttggaagac cggattatct tttcttaggt
     2641 agaccgcatc cgcctgccag aaagcatgat ctcccggtct tctcataata tccaccatat
     2701 cgatcagccg gaaacccaaa ccatcgagat aggaagagag ctcctggaat aacaggcagg
     2761 tcggagaaat gggaaatccg taaacttcaa tgatcagggc agaggttctt tttaatgctt
     2821 ccgttgtccc tttcaggatg ggaacttcat acccgtgtgt gtcaaactta attaaaatat
     2881 ttccttcgat tcctttgacc atttcatcga tggtaattac cgggactttc cgtacgttat
     2941 cccccgattg tgttccatat acgccgcttc cgtccagatc cggagccacg ttcagaaaga
     3001 cttccccgct tgcttctccg gcaacagcca gatggtagtt aattccggaa tgctttgatt
     3061 tcaggttctc cagcctgttc ctttgctctg ccagcggttc caccagttca tatctggcat
     3121 cgggccatac catcagggct ttttcggtcc aggttccctg cgcggctccg aggtctataa
     3181 ttatttcagg ccggatctcc cattgccgca gccgctgcaa ggccgccgcc atggatgccg
     3241 gatccccgga aagcactcca ttcttctgga tgctgtatcc ggccgatatg atcgcctttc
     3301 tgattatgga tttgagtaga gacattattc caggtgcccg aatttagcca ggcctttctt
     3361 tagcgaatcc gggaacaacc ggactgcgcg gatggccatc cgccgtaaag cagatttctt
     3421 tttccagctg ttcaatttgt cctgtgccag aacaatttct ttttttgcca tgtagctgca
     3481 tttgaacccg gaataaaaaa aataaccggc ggatttcagt tcttcattgt actgctcata
     3541 gatttcctta aggtcaggtc tttctttcag gttaaaccgg ttataaaacg gaacggacaa
     3601 ttccccggaa tcaattataa aagaactgaa atgaaagaat ctcaattttt cccggtcatt
     3661 cacaaaatat tccccattca ccctggaaag ataacgctca tgcaggttcc agggcgccat
     3721 attgtacccc atgcatttta atatgcccac gttattgaaa aacaggggca ccaggtttac
     3781 cggcaactga tcaacgaata atccctcgct tatcctgata taccccaggc tatacgtcct
     3841 ttccttccac cattttaaaa attttagcgt ctccccgccg ttttttaagc ccagaaaacc
     3901 caggttgtag atcccataat ttagaaataa attttcgctt ggaatcttgc cgtccaacgg
     3961 gatcggggtg taaatatgag gagtcagaac gataccatac tggctaaatg cctcttccag
     4021 ttcagtgaat ggcgcaaaga ccctgatgtc cggatcaagg taaatcacgg tcccggcaga
     4081 ccgtttcaga aacaaaaatt caatgacccc gggcttaacg gaggtattta attcaatgat
     4141 attgtacttt tgggcaagct cgtcaaaatc cggaatgatc aggttaacgg gcaacacttc
     4201 aaacgggatc gagtcatagt caatccggtt atccttttca tccaccaggg caatgagaaa
     4261 gcaaaagcgc ggttcatgaa gtttcaggga gttgcctaaa acaattgcct gggacaggta
     4321 gttattggaa caaatagtga aaacgaaatc catgcgatcg cttatttgga tacccggtaa
     4381 ccaaaaatat tgagttgcag atcataggtc ggccggtaat cctcatgcag aaaaggattg
     4441 ttcatattat tccatgcttc tttaatatag accctgaacc cggcctcgtt caggatggaa
     4501 agcaaccggt ccaaatcctg gcctttatcc ggcaggctgt gatattcaac aaataaatgc
     4561 ttcacattat gcagcagatt ggcacaatcc gtcaatactt cgaattccgc cccttcaata
     4621 tcaattttta aaaaatcaac cggttcgttt atatagtctc tcaggcgggt tgcctgaacc
     4681 tggatagtgg tttcagcgga atggtccgaa aggcgtccac ccaggcttcc ggtaaccgca
     4741 aaattcaaca tctcctcttt gacccagacg gctttacact gcacatcgac atctccgatc
     4801 tcaaaagttt caaggttccg gatcaattta ttataaatac tgaaatccgg ttcgaaggca
     4861 acaattctcg ctttggggaa gagccgctta aaataaatta tgctgagccc gatattggta
     4921 ccgcaatcca gtatgtatgg tttctccgaa aatgaccgga accggtaaac ctcgtccaca
     4981 aaaatttctt tgagcgaatg aaggtaccaa aacgagtcgg tgatcaacat ttttctgccc
     5041 atcaggcagg agtcttcctg gttagtatat ttcagccgga cgagctctct caggctgaga
     5101 aatttgttct tcaggagatg ggcgtgttcg tcctttttca ggcgcaataa agcagcattc
     5161 ttcaggtatt taaatcccgt gttcaggccc ggtaattttg agatcatgga acgtcccccc
     5221 ttcttaaact ttggaattaa atagaattta ataaacgcct tatacagcaa cccggccgct
     5281 ttcattctgg aaatcgaatt tccgatcccc aattcataat taacgaaatc ctgctgttcc
     5341 tttgtgaccc gggtatgaaa accggcaaac aggcccctgg ccttctgata ggtcggctgt
     5401 tgtttataac tctgatagga gccg
//
LOCUS       DASYCU010000440         4574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3864927, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000440 DASYCU010000000
VERSION     DASYCU010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3864927"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VGM24_08865"
     CDS             <1..431
                     /locus_tag="VGM24_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4155527.1"
                     /translation="VYRHKLGWAEAEPLYPDFISGRELVSFYRDVRKASSRQTDKLIN
                     LFGMGQYLSTQTGSWSSGMIKRLSLLLAFIGDPALILLDEPLATLDNEAVLLLPELIR
                     EYHVNNGISFIFSSHYSFQADSFPVEKKLLIRDQTVHLMA"
     gene            428..1537
                     /locus_tag="VGM24_08870"
     CDS             428..1537
                     /locus_tag="VGM24_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155528.1"
                     /translation="MNITPVILFKSFIKPFYRRNAGLLSFLLFMMTAAVGRANEAGLL
                     EYHYALIRGLMLNQIILLLVLGIWFLYATKCEEFIVLTLRRAEFSFLNILSLVHPKRV
                     FRLLLLVQVLLFLPVILYALLIMAVGFHEHWYGQVITIGLFNLGICAAGARWYLYLLQ
                     NPGTTRPILKWRMPSVFGERFYRNFLIRYVLMRRKALLFAIKLYSCGILYLMLVNRVP
                     AEYDLNMIFLFYSFGLLAHGVLIHQIRQMEETRLTFYRGLPVPLFSRWVQYGWLYLFL
                     FIPEILIIGWLTPAHLNFSDAFLFVFFGYSILLLLNSILFIKSVRMTDYLKILVAVFF
                     LLFIAVLTGMVLWLSVFSSILAARLFFRSYYRFER"
     gene            complement(1519..2382)
                     /locus_tag="VGM24_08875"
     CDS             complement(1519..2382)
                     /locus_tag="VGM24_08875"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEY4155529.1"
                     /translation="MNIFSKLSNGWTLSMNSLKVLKENKQLILFPILSGLSMILLIGS
                     FIVAILASAGWDAANIGQEGTAVNYITGFLFYLVNYFIVVFFNTALIQCTRDYFNGEE
                     PSVRKGLQFSVSRIGAILSWSLFAATIGFALRILQENLGTLGKIITGLIGVVWSIASF
                     FVVPIIAYENLGPLQAFKRSVALMKEKWGESLGSTFSFGLLRLLAFCVICGPLFFLGL
                     IIHPLLGIGLAVLGAFLVMAVLSAAQTIFISAVYHNLNGDPVKNFNEKFAENLFTEKK
                     QDPPGFYRSNR"
     gene            2913..4469
                     /locus_tag="VGM24_08880"
     CDS             2913..4469
                     /locus_tag="VGM24_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY4155530.1"
                     /translation="MRKLKIGIIDLVSKGPTHSLWARVMHANMASIMPQVVAAWCEQE
                     GHEVKMICYTGLEDLSKELPKGVDLLFISSFSQAAMLAYSISNYFRSQGTVTVLGGPH
                     ARCYPDDAVKYFDYVLGFTDKTVIRNILENFSFHKPVGVNLSATRQPVELPGVRERWK
                     FIEASLRKAPFFKMVPMIGSMGCPYTCSFCIDSTVSYQPLDFDTIQHDLRFLLTKFKK
                     PLVAWHDPNFGVRFEDNMRAIASAAPPGSFQFAAESSLSILTEDHLKVMQQNGFNAML
                     PGIESWYELGNKSKTSHIAGKEKLQKITEHVNMMFRYIPYVQTNFVLGLDSDSGEEPF
                     ELSKRFIDKSPGAFPGYSLLTSFGEAAPLNLQYQRENRVLPFPFHFLNNHLAMNMKPK
                     NYEWLDFYDKVIDLTAYSFSKKAIYRRYMATPHFTSKWLNFLRAISSEGYGRLRFYNR
                     IRKNLKEDRQFRSFFEGETTQLPSFYRDIVQHDLGAWWSWLPSGALEHNPNAYLHKSH
                     GQNAKVKQLA"
BASE COUNT         1140 a         1106 c         1087 g         1241 t
ORIGIN      
        1 ttgtttaccg gcataagctg ggttgggccg aagcagaacc tttatatccg gattttatca
       61 gcggccgcga attagtgagc ttctaccggg atgtccggaa agcatcttcc cggcagacgg
      121 ataagctgat caatttattc ggcatggggc aatacctttc cacgcaaacc gggtcctggt
      181 ccagcggcat gatcaaacgg ctgtccctgc tgcttgcttt tatcggcgat cccgcgctga
      241 tcctcctgga tgaaccgctg gccaccctgg ataatgaagc agttctttta ttacccgaac
      301 tgatcaggga gtatcacgtc aataacggca tcagttttat attcagctct cactattctt
      361 tccaggcaga ttcctttcct gtagaaaaaa aattattgat ccgtgatcag accgttcacc
      421 tgatggcatg aatattacgc cggtcatact atttaagtcc ttcatcaagc cgttttaccg
      481 ccggaatgca ggtctgctca gcttcctgct ttttatgatg accgctgcgg tcggaagggc
      541 aaatgaagca ggccttctgg aatatcatta cgctctgatt cgcggattaa tgctaaatca
      601 gatcattctt ttattggtcc ttggcatctg gtttctttac gcgacgaaat gtgaggagtt
      661 tatcgtcctt acccttagaa gagcggaatt ttccttcctg aatattttgt ctctcgttca
      721 tcccaaaagg gtcttccggc tgctgcttct ggttcaggtt ctgctctttt taccggtgat
      781 attgtacgcc ctgctgatca tggcagttgg ttttcacgag cactggtacg gacaggtgat
      841 caccataggg ctttttaacc tggggatttg tgcggcaggc gcccgctggt acctttatct
      901 tctgcaaaat ccgggaacta cccggccgat cctgaaatgg agaatgccat ccgtatttgg
      961 agaacggttt taccggaact ttttaatccg ctatgtgctg atgcggcgga aggccctttt
     1021 atttgccatc aagctgtata gttgcggcat cctttacctg atgctggtaa accgggtgcc
     1081 cgcggaatat gatctgaata tgatttttct cttctacagt tttggattgc tggcgcatgg
     1141 cgtgctgatt caccagataa gacagatgga agaaaccagg ctgacttttt accggggatt
     1201 gcccgtacca ctgttcagcc ggtgggtcca gtatggatgg ctgtatctct tcctcttcat
     1261 tcccgaaatc ctgatcattg gatggctgac gccggcccat ctgaattttt cggacgcttt
     1321 tttgtttgtc tttttcggat acagcatcct gctgttgtta aacagcatcc tgtttatcaa
     1381 atccgtccgc atgacggact acctgaagat cctggtggcg gtgttcttcc ttttgttcat
     1441 cgccgtactg accggaatgg tactgtggtt atctgttttt tcttctattc tggcggcccg
     1501 tctcttcttc cgtagctact accggtttga gcggtagaaa ccgggcggat cctgcttttt
     1561 ttcagtgaag aggttttccg cgaacttttc attgaagttt tttaccggat ctccgttgag
     1621 gttatggtac acggcgctga tgaaaatggt ttgggcggcg ctgaggacag ccattaccag
     1681 gaaagcacct aaaacagcca acccaattcc taaaagcgga tggatgatca ggcctagaaa
     1741 gaaaagcggc ccgcaaatca cgcaaaaagc aagcaatctg agtaagccga agctaaaggt
     1801 ggatcccagg ctttctcccc atttttcctt catgagggca acagaccgtt taaaggcctg
     1861 cagcggtccc agattttcgt aagcaataat gggaacgaca aaaaagctgg caatactcca
     1921 aaccaccccg atgagtcccg tgatgatctt tcccagggtt cccaggtttt cctgcagtat
     1981 ccggagggca aagccgatag tcgctgcaaa tagcgaccag gacaaaatag cgccgatccg
     2041 gctcacgctg aactgaaggc cctttctcac ggaaggttct tctccattga aataatcccg
     2101 ggtgcattga ataagcgcgg tattaaaaaa aacgactatg aaatagttga cgagataaaa
     2161 aaggaaaccg gtaatataat tcaccgccgt tccttcctgc ccgatattgg cggcatccca
     2221 cccggcagac gccaggatgg ccacgataaa agagccgatg aggagtatca tcgataagcc
     2281 ggataagatc gggaacagga tcaactgctt gttttccttc aaaaccttga ggctgttcat
     2341 ggacagcgtc cagccattcg ataatttgga gaagatattc atttgaacag gtttgggtaa
     2401 ggataaatat aataaagatt gttaacaaaa gctacccggg ccaccgagcc gtaattgagc
     2461 ccgagtctgt cccgtttatc cggtaagagt aatttacagc gcagggaaac catatttttt
     2521 atataaacct tcaaaccgcc gaaaaacctc ttttggtccg gtgtttttcg ctaaattact
     2581 aaattattgg ttcggcggaa agccggaaca tttgggtccg tggaagatcc ggccggtatt
     2641 taacttaatt ccggttattt ttttagctgc tttttatggt agtggactgc gttaatagta
     2701 ttccgttaca cttgttcaat gattttaaag atttaacaga aaatgagcca gggcattccg
     2761 cccggaactt aaactgtgaa ttatataaca tcctggggga ataatgtaat gttatgtgaa
     2821 taaaaacgag catctttaat tgactgaaag gccgtttgcc gggaatggga aggttcccgc
     2881 cagcagtccg ccggtccata aaatcactga gcatgcgaaa acttaaaatt ggtattattg
     2941 atttggttag caagggacca acacatagcc tgtgggcaag ggtgatgcac gcaaacatgg
     3001 cgagtattat gccgcaggtg gtggcggcct ggtgcgagca ggaaggccat gaagttaaaa
     3061 tgatctgtta taccggcctg gaagatttaa gcaaagaatt accaaagggc gttgacctcc
     3121 tattcatcag ttcgttttcc caggctgcta tgctggctta ttccatcagc aactatttcc
     3181 gcagtcaggg tacggttacg gtactgggcg gacctcatgc ccgctgttac cccgacgatg
     3241 cggtgaaata ttttgattat gttctgggat tcaccgataa aacggtcatc cggaatatcc
     3301 tggagaattt cagttttcat aagcccgtgg gagtaaatct ttctgcgacc cgccagccgg
     3361 tcgaactgcc cggcgtgcgg gagagatgga aatttattga agccagcctc cggaaagcgc
     3421 cttttttcaa aatggtcccc atgatcggaa gcatgggttg tccttatacc tgttctttct
     3481 gcattgattc gacggtctct tatcagccgc tcgattttga taccattcag cacgacctcc
     3541 gcttcctgct tacgaaattc aaaaagcccc tggttgcctg gcacgatccg aatttcggag
     3601 tccgctttga ggacaatatg cgggctatag catccgctgc accacccgga agcttccagt
     3661 tcgctgcaga aagcagcctt tccatactga ccgaggatca tctgaaagta atgcagcaaa
     3721 acggattcaa tgcgatgctc ccgggcatcg aatcctggta tgaactcggt aataaatcga
     3781 aaacttcgca tatagccgga aaagagaaac tgcaaaagat cacggagcat gtgaatatga
     3841 tgttccggta tattccgtat gtacagacta atttcgtgct ggggctcgac agcgattcgg
     3901 gagaagagcc ctttgaactc agcaaacgat ttatcgacaa atctcccggg gcttttcccg
     3961 gttattccct gctgacctcc ttcggcgaag ccgctccgct caacctgcag taccagcgtg
     4021 aaaaccgggt gcttcctttt ccttttcatt ttctgaataa tcatctggcc atgaatatga
     4081 agcccaagaa ctatgagtgg ctggatttct acgataaggt gattgatctc acggcataca
     4141 gcttttcaaa aaaagccatc taccgccgct atatggccac tccccatttc acttccaaat
     4201 ggctgaactt tttgcgggcc atttcttcag aaggatatgg acgcctccgg ttttacaacc
     4261 gcatccggaa aaatttgaaa gaggaccggc aattcagaag tttttttgaa ggagaaacaa
     4321 cgcaattacc ttcattctac cgggacatcg ttcagcatga tctgggagcc tggtggtcct
     4381 ggcttccgtc cggtgccctg gagcataacc ccaatgccta tctccataaa tcccatggcc
     4441 aaaacgccaa ggtcaagcaa ttagcctgat ccattgttca ttggcattcc gccggattca
     4501 aaacgccctc ctgagatcag ggcacattcc ctattcaaac aggcagcggc gaaaaaaccg
     4561 gaagcttccg ggcc
//
LOCUS       DASYCU010000441         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1981359, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000441 DASYCU010000000
VERSION     DASYCU010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981359"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VGM24_08885"
     CDS             complement(<1..966)
                     /locus_tag="VGM24_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HEY4155531.1"
                     /translation="MASRYRFNRVYSRLRKMIFSLLIIAGFQAAAQTGPRSRVSFNEG
                     WKFFLGDVPEAIAQNFSDANWRSLQLPHDWSIEGTFSEKNPATVGGGALPGGIGWYRK
                     SFLVPGTDRGKNIFIDFDGVYCNSEVWINGHYLGKRPNGYISFRYDLTPYLEFGDKKN
                     EIAVKVDNSHQPNSRWYSGSGIFRNVWLVTTNKIYVDHWGSFVSTPEISDRSAEVNLS
                     LQVHAPENYNKPLLVSTTVLNESGKEVANARTEVKLVKDTRLNLSQHFAIPNPELWSV
                     DRPYRYRAVTRIMTDGKESDEYSTPFGVRYFSFDADNGFFLNGKSL"
     gene            1068..2249
                     /locus_tag="VGM24_08890"
     CDS             1068..2249
                     /locus_tag="VGM24_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155532.1"
                     /translation="MTYIYSKLRCSPMSETSPARLRKAIAVYFFISGFGYSSWTSRIP
                     VVKETLKLNDAHFGTLLFMMPVGLIITMPFTGKLLDHFKSRVIMLAGALIYFVVLVFL
                     GFSPFAWMLAIVLFFFGASRNLMNLSMNAQAIGVQALYPRSIMSSFHAVWSLAGFAGA
                     AAGYVMVATHVIPGWHLLGVSLLLTSLTLYYYKDTLDQKPDMNNRRPIFSLPPKGMLI
                     FSLICFASMACENTMYDWSGIYIRQVLHGSNGVATIAFVIYMVAMTTGRIFGDRVADK
                     FGIQFVLRASGILIFCGLLITVISPFIPLTLAGYLLTGFGVSCVVPFIFSLAGKIPMS
                     NPGPALASISSIGYLGFLLVPPMIGYVAQASSLRISFAIIATMGLLIIRLSKKMEPLP
                     G"
     gene            complement(2281..3030)
                     /locus_tag="VGM24_08895"
     CDS             complement(2281..3030)
                     /locus_tag="VGM24_08895"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEY4155533.1"
                     /translation="MNPSAHLKFVIGAGIVFLLLFSPGSLLAQKPPGEAKTLHVSKCN
                     DFTITGNGTDPEWKKTAWHALTRLDSAGENYATRFKILYSGKGIYVLFDGQDKRITTT
                     YDQDFQDLYNADVFEVFFHPDTTMPLYLEYEINPLDKELVLLIPNLGRRVSGWLPFHY
                     TNGRRVEKRVNIEGGKGEPGASIRSWSAELFFPYGIFDPLNNVPPASGTVWHANFYRL
                     DYDSGSMVKWSWAPIEHTFHEFRKFGSIVFE"
     gene            complement(3034..>3260)
                     /locus_tag="VGM24_08900"
     CDS             complement(3034..>3260)
                     /locus_tag="VGM24_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155534.1"
                     /translation="FFSEKWAYLVYETDKGSKPYAPVLSWVKKLTHPLRIQLNEEVDD
                     RVSRTVTELFVDKGKSGWENRETSNHHTKK"
BASE COUNT          759 a          832 c          751 g          918 t
ORIGIN      
        1 cagggacttc ccattgagaa aaaagccgtt gtccgcgtca aatgaaaaat accggacgcc
       61 gaacggggtg ctgtattcat cactttcttt tccatcggtc attattctcg ttactgcgcg
      121 gtaccggtaa ggccggtcga cagaccagag ctccggattc gggatggcga agtgctggga
      181 aagattcagc cgggtatctt tcaccaattt tacttctgtt cttgcatttg ccacttcctt
      241 tccggattca tttaaaacgg tagtggaaac aagcagcggt ttattgtagt tttccggagc
      301 atgcacctgc aggctcagat taacctctgc ggagcgatcg gaaatttcag gtgtgctgac
      361 aaaagagccc cagtggtcga cataaatttt attggtcgtg accagccaca cattccggaa
      421 aatacccgaa ccggaatacc accgggaatt gggctggtgc gaattatcca cttttacggc
      481 gatctcattt ttcttatccc caaactccag gtagggagtc aggtcgtacc ggaatgaaat
      541 atatccattt ggcctttttc cgagatagtg gccgttgatc catacttcac tgttacaata
      601 aacgccgtca aaatctataa aaatattttt gcccctgtcc gttccgggaa ccaggaatga
      661 tttgcgatac cagccgatcc ctccgggcag ggctcccccg ccgaccgttg ccggattttt
      721 ttcgctgaat gttccttcaa tgctccagtc atgcggcaat tgcagggagc gccagtttgc
      781 gtcagaaaaa ttttgggcaa tggcttcggg aacatctccc agaaaaaatt tccaaccctc
      841 attgaacgat acacggcttc ttggaccggt ctgtgcagca gcctgaaaac cggcaataat
      901 taagagggaa aaaatcattt ttcttaaccg ggaataaacc cggttgaacc ggtatctgct
      961 tgccatttta aggtatttga tataaatata aaatgtttgg ttcacagcgt ctttcatttt
     1021 cccaaaaaaa aacattgccc tggctcaaat cggctgaaaa aggatagatg acctatattt
     1081 acagcaaact ccgctgttcc ccgatgtccg aaacttcccc agcccgcttg agaaaggcaa
     1141 ttgctgttta cttttttatt tccgggtttg gatattccag ttggacctcc cgcattcctg
     1201 tggtaaaaga aacgcttaaa ctcaatgatg cgcatttcgg caccctgctg ttcatgatgc
     1261 cggtcgggct catcatcacc atgcctttca ccggaaaact gctggatcat tttaaaagcc
     1321 gagtcataat gttagccgga gcactgatct attttgtggt cctggtcttt ctcggattca
     1381 gtccctttgc ctggatgtta gccatcgtat tatttttctt tggtgcctcc cgcaacctga
     1441 tgaatctttc catgaatgcg caggccatcg gggtgcaggc gctgtacccg cgttccatca
     1501 tgagttcatt ccatgctgtt tggagcctgg ccggttttgc cggcgcggcc gcgggatacg
     1561 ttatggttgc aacgcatgtt atcccgggct ggcatttgct gggggtgagc ctgctgctga
     1621 cctcgctgac cctttattac tataaggata cgcttgacca aaaaccggat atgaataacc
     1681 ggaggcccat cttttcactg cctccgaaag gaatgctgat cttttccctg atttgctttg
     1741 catcgatggc ctgtgaaaat accatgtacg actggagtgg aatttatatc cggcaggtgc
     1801 tgcatggttc aaacggcgtg gccacgattg cctttgtcat ttatatggtg gccatgacga
     1861 ccggaagaat attcggtgac cgggtggcgg ataagtttgg aattcaattt gttttgagag
     1921 ccagcggcat tcttatattt tgtggattac tgatcacggt aatttctcct ttcattcccc
     1981 tcacgctggc gggctacctg cttaccggct ttggtgtctc ctgtgtggtt ccttttatat
     2041 tcagtctggc cggcaaaatt ccgatgtcca atcccggtcc ggccctggcc tccatttctt
     2101 ccatcggcta cctggggttt ttactggtgc caccgatgat cggatatgtt gcgcaggctt
     2161 caagcctccg gatatctttc gctatcatcg ccaccatggg attattgatc atccggctga
     2221 gtaaaaaaat ggagccactg cccggctagc ggctatcccg taattgcagt cgcggtcttt
     2281 ttattcaaag acgatggatc cgaatttcct gaattcatga aaagtatgct caatgggagc
     2341 ccaggaccat ttcaccatgc tgccgctatc atagtcaagg cggtaaaaat tggcatgcca
     2401 taccgtaccg cttgccggag gaacattatt cagcggatca aagatcccgt aggggaaaaa
     2461 cagttccgcg ctccaggatc tgatggaagc acccggctcc cccttgcctc cttcgatatt
     2521 cacccgcttc tccacccgtc tcccgttcgt atagtgaaaa ggaagccatc cggatactct
     2581 cctgcctaaa ttcggaatga gcagaaccag ttctttatcc agcggattta tttcgtattc
     2641 taaatataac ggcatggtcg tatcgggatg aaagaaaact tcaaacacat ccgcattgta
     2701 gaggtcctga aaatcctggt cgtaggtcgt ggttatccgt ttatcctggc cgtcaaaaag
     2761 cacatagatc cccttcccgg aatataatat tttaaaccgc gtggcataat tctcccctgc
     2821 tgaatcgagg cgcgtcagcg catgccaggc cgtcttcttc cattccgggt cagttccgtt
     2881 tcccgtaatc gtaaaatcat tgcattttga aacatgcagc gttttcgcct ctcccggagg
     2941 tttctgcgcc aacaggcttc ccggtgaaaa cagcaacagg aaaacaatcc cggctccgat
     3001 gacgaatttc agatgtgcgg atggattcac cgtttatttt tttgtatgat ggttactggt
     3061 ttccctgttc tcccaaccgc ttttcccttt atcaacaaac agttccgtaa ccgttctgga
     3121 aacccggtca tccacttctt cattcagctg gatccgcagc ggatgagtaa gtttttttac
     3181 ccaggagagc accggagcat agggcttgct gcccttatcc gtttcataga ccaggtaggc
     3241 ccatttttct gaaaagaaat
//
LOCUS       DASYCU010000442         4287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4446516, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000442 DASYCU010000000
VERSION     DASYCU010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4446516"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGM24_08905"
     CDS             <1..501
                     /locus_tag="VGM24_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155535.1"
                     /translation="YKILSDSLIKAVLSSRDSSLQMQDAWKSYLSWKKSTGPDAVSAM
                     GLNDETNARHFIRAQLGMMGNSWFVYFLQTDPADFISKLHCKVLALNGSKDIQVLADQ
                     NLAGIERALKKSAVKNYSVKKIEGLNHLFQHCKACTIPEYGQIEETFAPEVLQIMGDW
                     LDKNVK"
     gene            503..1093
                     /locus_tag="VGM24_08910"
     CDS             503..1093
                     /locus_tag="VGM24_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HEY4155536.1"
                     /translation="MNYLAHAYLSFSDPDILAGNMISDFVKGKKKFEYPLKVQNGISL
                     HRKIDEYTDYHPATQQAKQFLKEACGLYAGSFIDIVYDHFLANDLHEFEEGALAVFAQ
                     KTYEQLDDYKNLLPEKFERFLFYMREQNWLLNYRSTDGIHRSFQGLTHRAKYIRDAEP
                     PFQAFLRHYESLKNCYELFFPNLKTFAYTELLYLRP"
     gene            1142..1705
                     /locus_tag="VGM24_08915"
     CDS             1142..1705
                     /locus_tag="VGM24_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEY4155537.1"
                     /translation="MKSAFFGMLMIVGFAGAHAQTAPQPLDKSPMDMSYYPVNYPILK
                     IQHKADAPLIARVIYGRPQKNNRVIFGDLVPYHSVWRLGANEATEIEFFRDVKINNKK
                     LAKGKYTLYAIPDTTQWTLILNKETDTWGAFDYDAKKDVLRVDMPVKKLTENVNVLTI
                     CFEGDKKNSTDLCMSWDDQEVHMPISW"
     gene            1762..2901
                     /locus_tag="VGM24_08920"
     CDS             1762..2901
                     /locus_tag="VGM24_08920"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY4155538.1"
                     /translation="MKFATRLIHAGMEPDPSTGAIIPPVYQTSTFVQAAPGNNKGYEY
                     ARSQNPTRTALEKALASIENARHALCFSSGVAASDAVIRLLVPGDEVIAANDMYGGTF
                     RLFSKIFEKAGIRFHYINMQDPDNLKPFINAKTKLIWTETPTNPLMNIVDLAAVSGIA
                     KEKNILLCVDNTFASPCLQNPLDLGADIVVHSATKYLGGHSDVIHGCVMLNDNALREQ
                     LFFIQKSCGAVPGPQDCFLVLRGIKTLQVRMDRHCLNGEKVARYLRKHPKVARVYWCG
                     FEDHPGYTVAKKQMRGFGGMLSFELKNDSPEETKRVLSATKLFSLAESLGGVESLMSH
                     PASMTHASIPREERLKYGLKDNLIRLSVGIEDADDLVEDLDKAIG"
     gene            2936..3718
                     /locus_tag="VGM24_08925"
     CDS             2936..3718
                     /locus_tag="VGM24_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HEY4155539.1"
                     /translation="MYELNKNELCDFLESKVQQYNNPSFISTDPVSVPHRFGKKQDIE
                     IAGLFAAIFSWGNRTTIIQKSNELMKAMDHAPHDFVMNHQPRDLKKLLEFKHRTFNTT
                     DLLYFIRFLQSHYRQNDSLETAFIPAESGKKDRMKNGLSSFYTRFFSLEDAPRRTQKH
                     ISSPQKNAACKRLNMYLRWMVRRDNKGVDFGLWKKLSPADLICPVDLHVARVARRFGL
                     ISRNQTDWTTAMELTTALRTMDKKDPVKFDFALFGLGIFEKY"
     gene            complement(3731..>4287)
                     /locus_tag="VGM24_08930"
     CDS             complement(3731..>4287)
                     /locus_tag="VGM24_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY4155540.1"
                     /translation="VPVIIYFAYLSHFRFELSYGMMFGMFAAGIFFWTFFEYLMHRFA
                     FHAVGESERAKKFIYIMHGNHHHFPRDKERLFMPPVPSIIISSLIFGIMFLAMKEYAF
                     TFFSGFIFGYLMYGSIHYAIHAWNPPFKWMKPLWRNHHLHHYKDDNKGFGVSTTIWDR
                     VFGTMFDLGREKEDKEKVRELMYK"
BASE COUNT         1157 a         1074 c          999 g         1057 t
ORIGIN      
        1 tataaaatat taagcgattc gctcattaag gcagtcctgt cttcccggga cagcagcctg
       61 caaatgcagg acgcctggaa atcttatctg tcctggaaaa aaagcaccgg tccggacgcc
      121 gtttccgcca tgggtctgaa cgatgaaacc aatgcaagac atttcatccg ggcacagctg
      181 ggcatgatgg ggaattcgtg gtttgtctat tttcttcaaa ccgacccggc cgattttatc
      241 agtaaactgc actgcaaagt gcttgccctg aacggctcga aagatattca ggtgctggct
      301 gaccaaaacc tggcgggcat tgagcgggcc ctaaaaaaaa gtgcggttaa aaattattct
      361 gtgaaaaaga tcgagggtct caatcatctg ttccagcatt gcaaagcctg cacgatcccc
      421 gaatatggtc agatcgaaga aacgtttgcg cccgaagttt tacagatcat gggtgactgg
      481 ctcgataaaa atgtaaaata aagtgaacta cctggcacac gcctatctct ctttttctga
      541 tccggacatt ctcgcgggaa atatgatcag cgattttgtg aaaggcaaaa aaaagttcga
      601 atacccgttg aaagtgcaaa acggcatcag tcttcaccgg aagatcgatg aatacacgga
      661 ttaccatccg gctacccaac aggccaagca atttctgaaa gaagcctgcg gtttgtatgc
      721 cggaagcttt atagatatcg tatacgatca cttcctggcc aacgacctgc atgagtttga
      781 agaaggcgcg ctggcggtct ttgcgcagaa aacatacgaa cagctggatg actataaaaa
      841 tttgctgccg gaaaaatttg aacgttttct tttttatatg cgcgagcaaa actggttatt
      901 gaattaccgc tcaacggacg gcattcaccg cagttttcag ggcctcacgc accgggcaaa
      961 atacatccgg gatgcagaac ctcctttcca ggccttcctc aggcattatg aatcgttaaa
     1021 gaattgctat gaacttttct tccccaactt aaaaactttt gcttacaccg agttgctcta
     1081 tcttcgcccc tagactaagc ggaaatactt agttttacct tcttttttaa tttgaaataa
     1141 tatgaaatct gcctttttcg gaatgctgat gatagttggt tttgccggcg cgcacgcaca
     1201 aacggctccg caacccctgg ataagtcgcc catggacatg agttattacc ccgtcaatta
     1261 tcccattctt aaaatacagc ataaggcgga tgcacccctg atcgcccgcg tgatctatgg
     1321 ccgtccgcaa aagaataacc gggttatttt cggcgacctg gttccctacc acagcgtatg
     1381 gcggctgggg gctaacgaag ccacggagat cgagtttttc cgggatgtga aaatcaataa
     1441 taaaaaactg gccaaaggaa aatatacctt gtatgccatt ccggatacca cccaatggac
     1501 cctgatccta aacaaagaaa ccgatacctg gggggcattt gactatgatg ccaaaaaaga
     1561 tgtgcttcgc gtggatatgc cggtaaaaaa attaaccgaa aatgtaaatg tcttaaccat
     1621 ttgctttgaa ggcgataaaa aaaatagtac ggatctgtgc atgtcctggg acgaccagga
     1681 agttcacatg cccattagct ggtaatccgt tcctttgtat cgccggaccc ggtgactgac
     1741 ctttcttaaa aacatttgct catgaaattt gctacccgcc ttatccatgc tggcatggag
     1801 cccgatccca gtacaggggc cattataccc ccggtttacc agacctccac ttttgttcag
     1861 gcagccccgg gcaataacaa gggttatgaa tatgcacgtt cccagaatcc cacccgcacg
     1921 gcgctggaaa aagcgctggc cagcattgaa aatgcccgcc atgccctgtg cttcagcagc
     1981 ggggtggccg cctctgatgc cgtgatccgg ttgctggtcc ccggcgatga agtgatcgca
     2041 gccaacgaca tgtatggcgg cacttttcgc cttttctcca aaatatttga aaaagccggt
     2101 atccgctttc attatatcaa catgcaggac ccggataacc tgaagccttt tatcaatgcc
     2161 aaaaccaaat tgatctggac ggaaacaccg accaatccgc tgatgaatat cgttgacctg
     2221 gccgctgttt ccggtattgc caaagaaaaa aacatcctgc tctgtgtgga caacaccttc
     2281 gcttccccct gcctgcagaa tcccctggac cttggggcgg atatcgttgt ccattccgcc
     2341 accaaatatc tcggcggcca cagtgacgtg atccatggat gtgtcatgtt gaacgataat
     2401 gcgctgcgcg aacaattatt ttttattcag aaatcctgcg gagcggttcc cggcccccag
     2461 gactgctttc tcgttttaag gggaatcaag accctgcagg ttcgcatgga caggcattgc
     2521 ctgaacggag aaaaagttgc ccggtattta cgcaaacatc cgaaagtagc gagggtttac
     2581 tggtgcggat ttgaagatca tcccggatac actgtggcca aaaaacaaat gcgcggattc
     2641 ggcggcatgc tcagttttga attgaaaaac gattctccgg aagaaaccaa acgggtgttg
     2701 tctgcaacta aattgttttc cctggcggaa agcctgggcg gagtggaatc cctgatgagc
     2761 cacccggcca gtatgaccca tgcttccatt ccccgggaag agcgcctgaa gtacgggctg
     2821 aaggataacc tcatccgcct gagcgtgggg atcgaggatg ccgatgatct ggtggaagac
     2881 cttgataaag caattggctg atcgccgttc cccggccttc aaccgacttt tatatatgta
     2941 tgaattgaat aagaatgagc tgtgcgattt tctggagagc aaagtgcagc agtacaataa
     3001 tccatctttc atttctaccg acccggtttc ggtaccgcac cggtttggca aaaaacagga
     3061 tatagaaatc gccggactct ttgccgccat cttttcctgg ggtaaccgga ccaccatcat
     3121 tcagaaatcc aatgaactca tgaaggccat ggaccatgcg ccgcatgatt tcgttatgaa
     3181 ccatcaaccg cgtgatctga agaaattgct cgaattcaaa caccggacct tcaataccac
     3241 cgacctgctt tattttatcc ggtttttaca atcccattat cggcaaaatg attccctcga
     3301 aaccgctttt atacctgccg aatccggcaa gaaagaccgg atgaagaacg ggctttcttc
     3361 tttttatacc cgattttttt ccctggaaga tgctccgcgg cggacccaaa aacatatttc
     3421 cagcccgcaa aagaatgccg cctgcaaacg gttgaatatg tatttgcgct ggatggtccg
     3481 gcgggataat aagggcgttg attttggctt gtggaagaag ctttcgccgg cagatctgat
     3541 ctgtccggtg gatctccatg tggcccgcgt ggccagacgc ttcgggctga tctcccgcaa
     3601 tcaaacggac tggacaaccg ccatggaact caccacggcc cttcgcacaa tggataaaaa
     3661 agatccggta aaatttgatt ttgccctgtt cggactcggc atttttgaga agtactgaac
     3721 cgggatgcgg tcatttgtac atgagctccc gcactttttc cttgtcctct ttttccctgc
     3781 ccaggtcgaa catggttccg aacacccggt cccagatggt tgtgctcaca ccaaaccctt
     3841 tattgtcatc cttgtagtga tgcaggtggt gattcctcca taaaggcttc atccatttaa
     3901 agggcggatt ccaggcgtga atggcgtagt gaatactgcc atacatgaga tatccgaaaa
     3961 tgaaaccgga aaagaaagta aatgcgtatt ccttcatggc caggaacatg atcccgaaga
     4021 tcagggaaga gatgatgatg ctgggcaccg gaggcataaa cagccgctcc ttgtcccgcg
     4081 ggaaatgatg atgattcccg tgcatgatat agataaattt tttggctctt tcactttctc
     4141 ctacggcatg gaaggcaaag cggtgcatca ggtattcaaa gaaagtccag aaaaaaatgc
     4201 cggcggcaaa catcccgaac atcattccat agctcaattc aaaccggaaa tgactcagat
     4261 aggcaaaata aatgatcacc ggcacat
//
LOCUS       DASYCU010000443         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3142178, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000443 DASYCU010000000
VERSION     DASYCU010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3142178"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VGM24_08935"
     CDS             complement(<1..1330)
                     /locus_tag="VGM24_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY4155541.1"
                     /translation="MEKQIIENRLSINRRKFLSRLSLGIGSVALGSLLIPDLFSGGAE
                     DDAASFMTGLPNFAPKAKRVIYLFQNGAPSQLESFDYKPLLRDMSGKDLPESVRMGQR
                     LTGMSAGQAHFPLAGSLFNFKQYGKSQAWISDLFPHTARIADDICIVKTLFTEAINHD
                     PALTFFQTGAQQGNLPSFGSWMSYGLGSENKNLPAFCVLLSRGRGNGQGVYSKLWSNG
                     FLDSIHQGVQFSSGDNPVLYLNDHNGIDKAGRRKMLDKLEEMNDISYKEFGDPEISTK
                     IQQYEMAYRMQTAVPDITDVSKEPDDIVKMYGPDCLLPGTYAANCLLARKLSENGVRF
                     VQLYHQGWDQHSNLPKEMPGQAMDVDQASSALVTDLKQRGLLDETLVIWGGEFGRTNY
                     SQGKLTKDNYGRDHHPRCFSIWMAGGGIKGGIVHGETDEFGYNIIKDPVHV"
     gene            complement(1390..3705)
                     /locus_tag="VGM24_08940"
     CDS             complement(1390..3705)
                     /locus_tag="VGM24_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HEY4155542.1"
                     /translation="MRKICLLLLLSVSAAVCLIRSCNNPTQASEKRSPEEISYNFQVR
                     PILSDKCYACHGPDANKRKAGLRLDLPGEAFKALKDNPDAHALVPGDPFHSELYLRVS
                     SKDSSEMMPPPSSNLSLTPEEIAILKKWISQGAKYQKHWAFTPPKQSALPKIEDKSWV
                     RNEIDYFIQDKLAQKGLKPNPEADRERLLKRICLDITGLPPSLEMMDQFSADTSSGAY
                     EKMIDRLLQSPAYGEKMAVHWMDLARYADSHGYQDDNYRTQWPWRDWVIHAFNENMSY
                     KQFVTWQLAGDLLPNANGEELLATGFNRNHKITEEGGAIDEEYRVEYVTDRTNTFGRA
                     FIGVSLECAHCHDHKYDPFSQKEYYQMFAFFNNVQERGLESTVGGPETYAKKPLMEIT
                     KDDLKGILSFVNKTDTNKLIVSVMGDSNKVRPTYVLKRGQYDSHGDTVEPGTPKAILP
                     FGDYPKNRLGLAEWLFDKRNPLTARVFVNQVWQELFGRGIVKTASNFGAQGELPSHPE
                     LLDWLSVDFVNHGWDIKYLVKKIMMSAAYRQSAVISPEKLKADPENMYLSRGPRFRLP
                     AEFIRDLVLSSSGLLVKTLGGPSVKIYQPAGLWEASTSGRGILATYIQDHGQSLYRRG
                     LYTFIKRTLAPPSMILFDASNRDQCEVKRSKTNTPLQALVMLNDPTVLEASRVLAGKL
                     LAEPTGVDDKIEKAFRLIVCRRPEAKEKQLLKEYYTDQLQSFTAQKQNARKLLAVGEY
                     PQNKKLDPVSQAAMMEVITTIYNLEETITKS"
     gene            complement(3942..>4488)
                     /locus_tag="VGM24_08945"
     CDS             complement(3942..>4488)
                     /locus_tag="VGM24_08945"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4155543.1"
                     /translation="IDSLYPPNTIQMYFTSNHDENSWNKADFGLFPGPSHAPFAVFTQ
                     TMAKGVPEIYSGQEEPVLRALAFFEKDPIHFEHFARAGFYKTLLSLRKKDIALSADAA
                     FKKVQAGDPKSIYAFLREKAGRKVLVILNFSNEEQTISVKDKSLWGNPYNVFMHNKEP
                     LSGRSWKMEPWGYAVYEYGGQ"
BASE COUNT          986 a         1202 c         1125 g         1175 t
ORIGIN      
        1 gcacgtgaac ggggtctttg atgatattat acccaaactc atcggtttcc ccatgcacta
       61 tgccgccttt tattcctcca cccgccatcc atatactgaa acagcgcggg tgatgatcgc
      121 gcccgtaatt atcttttgtc agtttgccct ggctgtaatt ggtgcggccg aattcaccgc
      181 cccagatcac cagcgtttcg tccagcaaac ccctttgttt taaatcggtc accagcgcgg
      241 aagaagcctg gtccacgtcc atggcctgcc ccggcatttc cttgggaaga ttgctgtgct
      301 gatcccaacc ctggtgatag agctgcacaa accggactcc gttctccgac agtttccggg
      361 cgagcagaca attcgcggca taggttccgg gcagaaggca atccggtccg tacatcttta
      421 cgatatcatc tggttctttg gatacatccg taatatccgg cacggccgtc tgcatccggt
      481 aagccatttc atattgctgg atcttcgtgc tgatctcggg atctccgaat tccttgtatg
      541 aaatatcgtt catctcttcc agcttgtcca gcattttccg gcgacctgct ttatctattc
      601 cgttgtggtc gttcagataa aggaccgggt tatccccgct gctgaactga actccctgat
      661 gaatggaatc caggaatccg ttgctccaca gtttggagta aacgccctgg ccgtttcctc
      721 ttccgcggga cagcagcaca cagaatgccg gaaggttttt attttcgctg cccaggccat
      781 agctcatcca ggagccaaaa ctgggcaggt ttccctgctg agcgcccgtt tgaaaaaagg
      841 tcagcgccgg atcatggtta atagcctccg tgaacagggt cttgacgatg cagatatcat
      901 ctgcgatcct ggcggtatgc ggaaaaagat cgcttatcca tgcctgtgat ttcccatatt
      961 gtttaaaatt gaaaagagaa cccgccagcg ggaaatgcgc ctggccggcg gacatgcccg
     1021 tcaaccgctg tcccatgcgc acggattccg gcaggtcctt gcccgacata tccctcagca
     1081 gcggtttata gtcgaaggat tccagctgcg aaggtgcgcc gttctggaaa agatagatca
     1141 cgcgttttgc cttcggggca aaattcggaa ggcctgtcat aaaggaagct gcatcgtctt
     1201 ccgcgccgcc gctgaaaaga tccggtatca ataaggaacc gagggcaacg cttccgatgc
     1261 caaggctcag ccgggacagg aatttgcgcc ggttaatact gagccggttt tcgattattt
     1321 gtttttccat ttgtcgttta acgtttcaca tggtgaacct tcatcccctt tccatccgcg
     1381 cctgttttct caggacttgg taatcgtttc ttccagatta tagatcgtcg tgatcacctc
     1441 catcatcgcg gcctgggaaa cgggatccag ttttttattc tgcgggtatt ctcccaccgc
     1501 cagtaatttc cgggcattct gtttttgcgc cgtgaaggat tgcaactgat ccgtataata
     1561 ctccttcaac aattgttttt cctttgcttc cggccttcgg catactatga ggcggaatgc
     1621 tttttcaatt ttatcatcca ctccggtcgg ctcggccagc agcttgcccg ccaggacccg
     1681 ggatgcctcg agaacggttg gatcgtttaa catcaccagc gcctggagcg gcgtattggt
     1741 ctttgacctt ttcacttcgc actgatcgcg gttgctggca tcgaacagga tcatcgatgg
     1801 cggcgccagc gtgcgtttga tgaaagtata tagaccccgc cggtaaaggc tctggccgtg
     1861 atcctgtata taggtggcca gtatcccccg gcccgaagtg gaagcttccc acagccccgc
     1921 gggctgatag atcttcacgc ttggccctcc cagggttttc accagcaggc cgctgctcga
     1981 caagacgagg tcccggataa actcggccgg taaacgaaag cgcggtccgc gggacaggta
     2041 catattttca ggatctgctt ttaatttttc cggagagatc acagccgact gccggtaggc
     2101 ggcggacatc atgatcttct tcacgaggta tttgatatcc catccgtgat ttacaaaatc
     2161 caccgagagc cagtccagta attccgggtg cgaaggcagt tcgccctgcg ccccaaaatt
     2221 gctggccgtt ttaacgatgc cgcgtccgaa gagctcctgc cagacctggt tcacaaatac
     2281 tcttgccgtt aggggattgc gtttgtcgaa taaccattcc gccaggccca ggcggttttt
     2341 cggataatcg ccgaagggaa gaatggcttt cggcgtgccg ggttccaccg tatcgccgtg
     2401 actgtcgtac tgccctctct tcagaacata ggtcggccgc actttattac tgtcacccat
     2461 aaccgaaaca atgagcttgt tggtatcggt cttgttgacg aaacttaaaa tacccttcag
     2521 gtcatcttta gtgatctcca tcaacggctt cttggcataa gtttccggtc cgccaacagt
     2581 agattccagc cctctttcct gaacgttatt aaagaaggcg aacatctggt aatattcctt
     2641 ctgcgagaag ggatcatatt tatgatcgtg acaatgcgca cattcgagcg aaacgccgat
     2701 aaaggcccgg ccaaaagtat tggttcggtc ggtcacatat tccacccggt attcctcgtc
     2761 gatcgccccg ccttcttccg ttattttatg attgcgatta aagccggtgg ccagcaactc
     2821 ttccccgttt gcattgggga gcaggtctcc cgcgagctgc caggtcacaa actgcttgta
     2881 cgacatgttt tcattgaagg catggatcac ccagtcgcgc cagggccact gcgtccggta
     2941 attatcatcc tggtaaccgt gagagtcggc atacctggcc aggtccatcc agtgaacggc
     3001 cattttttcg ccgtaagccg ggctttgtaa taagcgatcg atcattttct cataggcgcc
     3061 ggaagaggta tccgcgctaa actggtccat catttccagg gaaggcggca ggccggtgat
     3121 gtccaggcag atccgcttca gcaatctttc ccggtcggct tccggattgg gcttgagtcc
     3181 tttttgcgcg agcttatcct gaataaagta atcaatttca ttccttaccc agctcttatc
     3241 ttcaattttt ggtaaggcgg attgtttggg cggagtgaat gcccagtgct tttgatattt
     3301 cgccccctgg ctgatccatt ttttcagaat ggcgatctct tccggcgtca gggaaagatt
     3361 ggaagaagga ggcggcatca tttcggagct gtccttagag gagacgcgca ggtataattc
     3421 ggaatgaaag ggatcgcccg gaaccagggc atgcgcatcc ggattgtcct ttaaggcctt
     3481 aaaggcttcc ccgggaaggt cgagacgcag gccggccttc cgcttgtttg catccgggcc
     3541 atggcaggca taacatttgt cagagagaat ggggcgcacc tgaaaattat agctgatctc
     3601 ttcgggtgac ctcttttctg aagcctgggt ggggttgtta caggaccgaa tcaaacaaac
     3661 agccgctgaa acagacagca gcagcaatag gcatatcttc ctcatggcat tatcgttcaa
     3721 attggtttcc ggcaaggacg gatcggccaa ttttaccgaa aattaattaa ctatctgctc
     3781 ttttggaaac ttaattgtcc tccggaaacc ctcaagatca atattcgcca tgctaaggag
     3841 agcattatcc actttctctt tcagcccaaa ccctattcct gaaaattaaa gcaggccggc
     3901 atttagagaa cctgcggttc tcccggggat ccggcagtgg atcattgccc cccatactca
     3961 taaacggcat agccccaggg ctccattttc cagctccggc cgctcagcgg ttccttgtta
     4021 tgcatgaaca cattatatgg atttccccaa agcgatttgt ctttaacgga aatggtttgt
     4081 tcttcgtttg aaaaattcag gatgaccagc actttacggc cggctttttc ccgcagaaaa
     4141 gcatagatcg actttggatc gcctgcctga acttttttga atgccgcgtc cgcggaaagg
     4201 gcgatatctt ttttgcggag actgagcagg gtcttataaa aacccgcccg ggcaaagtgt
     4261 tcgaagtgga tcggatcctt ttcaaaaaaa gccagcgccc ggagaacggg ttcttcctgc
     4321 ccgctgtaga tttccggaac gcctttggcc atggtttgcg tgaatactgc aaaaggggcg
     4381 tgagacgggc cggggaacaa accgaaatcc gctttgttcc agctgttttc atcgtggttg
     4441 ctggtgaaat acatctggat cgtattcggc ggatagagcg aatcgatt
//
LOCUS       DASYCU010000444         5252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_385606, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000444 DASYCU010000000
VERSION     DASYCU010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385606"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGM24_08950"
     CDS             <1..205
                     /locus_tag="VGM24_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155544.1"
                     /translation="MSKSDKAIAIWVAFVDINSKKVLLTDRVEGKVGMGFGFRNYWAT
                     GIKSVLEQIEKKKYKEWKAKAGA"
     gene            227..658
                     /locus_tag="VGM24_08955"
     CDS             227..658
                     /locus_tag="VGM24_08955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY4155545.1"
                     /translation="MQNKLIVKTEILVRFNEADPLGIVWHGHYIRYFEDGREAFGNQY
                     GIGYLDFYKRGFVIPVVHVECSFKKSLRYGDSVVVETAYTPCDAAKLLFSYRLYNKKN
                     HQLVATGSSVQVFLDREHSTLQLTNPPFFESWKSKHLLSKS"
     gene            661..1782
                     /locus_tag="VGM24_08960"
     CDS             661..1782
                     /locus_tag="VGM24_08960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY4155546.1"
                     /translation="MRKVFVLADNIFSPLGKDTAGNLEALKQGRSGIRRHHSAVSNEP
                     FYASLLLEEDKTGDDYFTPFEQIIFHSVKDAMAGLDLDPANLRTGFILSSTKGNISAI
                     EEEGQGPVPESRVGLGSSAERIAKRLGIMSEPIVVSHACISGLLAMITGMRLIRTGAF
                     DEVIVTGADLITAFILSGFQSFQAISPEPCRPFDAARDGISLGEGAATVILSSRKKQG
                     AIQLSGGSVSNDANHISGPSRTGEELFLALKRALQQAGVSPGEIDFISAHGTATRYND
                     DMEAKAINLAGLGSVPLNSLKGFYGHTLGAAGLIESIVSIHSMKEDILFPTQGFTNPG
                     TEKPVNIIKELASAKIRKCLKTASGFGGCNAAIVLENPF"
     gene            1831..2913
                     /locus_tag="VGM24_08965"
     CDS             1831..2913
                     /locus_tag="VGM24_08965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4155547.1"
                     /translation="MNFYAKDKKSAIESKGLAQWIAFGPVVFQVARVLRNSGILKVLM
                     ECRSQGLTLEEIRDLVKLPHYGVRILLESGLGIGLVTIQDEKYFITKTGFFICNDPLT
                     VVNMDFVHDVCYKGLFALDKSIETGKPEGLKEFGNWDTIYEGLSSLPAQVQKSWFAFD
                     HFFSDIAFPLTLPLVYNGSIKKILDIGGNTGKWAIASAGYSKDLQITIVDLPGQLKVA
                     KEKVKQAGLEERIFFHPVNILDESASFPKGFDAIWMSQFLDCFSETEIVSILKRCHAA
                     LDDDKYVLILEAFWDDQRFETASFCLQQTSVYFTALANGNSQMYNSKVFIRCIEQAGF
                     EIEERTDNIGLSHTLLKCKKSKKLNQ"
     gene            2926..4176
                     /locus_tag="VGM24_08970"
     CDS             2926..4176
                     /locus_tag="VGM24_08970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4155548.1"
                     /translation="MPMNSTDVLVIGAGPAGTVAASIVNKAGYKVQIVEKMKFPRFVI
                     GESLLPRCMEALGEAGFLDAVKEKNFQEKSGAKFVKDGKICDYKFADQFTRGWQWTYQ
                     VPRSEFDKTLADTVEKMGVPVHYETTVTGIEFDGSDSVTTVLDKNGNASRIKAKFIID
                     GSGYGRVIPRLFNMDRPSNLPPRKALFSHTVDNRRAMSDEPNRITIVVHKPAVWIWII
                     PFSNGITSLGFVGDPEFFQQYPGTTEDQLRKLISVEPYLAERFDQVEFLFEPKTLQSW
                     SSTTDRFFGDGFVLTGNVTEFLDPVFSSGVTLATVSSQIAGKLVVEKLKGRNIDWENE
                     YMKPMMQGVDTFRSYVMAWYEGTLDTIFFADDQDELIKSQICSVLAGYVWDLENPYVK
                     NHKTSLQRLASIISYRDSLAAKKN"
     gene            4196..4822
                     /locus_tag="VGM24_08975"
     CDS             4196..4822
                     /locus_tag="VGM24_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155549.1"
                     /translation="MQSDRFITASCLIRANSAYKNGRQEFQRPGLNASDFLDELYRHE
                     QLAYPKFHKMDQLSKLGLLAADILLKDQDHQQKYAEDETGIVLSNSNGSLDVDLKYAK
                     TMKAGASPALFVYTLPNIVIGEIAIRHRFKGENAFFVFKSFNGNFIAEYVNGLFEDKL
                     VKCCICGWVDFLEEEYRALLMLVETSARGLAIPFTAADINQLNNATHG"
     gene            4815..5084
                     /locus_tag="VGM24_08980"
     CDS             4815..5084
                     /locus_tag="VGM24_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY4155550.1"
                     /translation="MDKLMATLKRQIIERLNLKDHKPEDIGDDQPLFVEGLGLDSIDA
                     LELIVLLQQDYNIKLSNAEDGPKVFHSVRTMAEYITEHSPSKISP"
BASE COUNT         1350 a         1325 c         1380 g         1197 t
ORIGIN      
        1 catgagcaag tcggataagg ccattgcgat ctgggtggct tttgtggata taaactcgaa
       61 gaaagtgctg ctgaccgacc gcgtcgaagg caaagtggga atgggattcg gtttccgtaa
      121 ttactgggcc accggcataa agagcgtgct ggaacagatc gaaaagaaaa aatacaaaga
      181 atggaaggcc aaagcaggcg cctgatccgt tcctatttac ctgcccatgc aaaacaagct
      241 gatcgtaaaa acagagatcc tggtgcgttt caatgaagcc gacccgctgg ggattgtctg
      301 gcatgggcac tatatccgtt attttgaaga cgggcgggaa gcgtttggaa accagtacgg
      361 catcggttac ctcgattttt ataaaagggg attcgttatc cccgtggtgc acgtggaatg
      421 ttctttcaag aagtccctgc gctacggaga cagcgtggtt gtggaaacgg cctatacgcc
      481 ctgcgacgcc gccaaacttt tattcagtta ccggctttac aataaaaaaa atcaccagct
      541 ggtggcgacc ggatcctccg ttcaggtttt cctggacagg gagcattcca ctctgcagct
      601 tacgaatccg ccttttttcg aatcgtggaa gagcaaacac cttctttcta aatcctgagc
      661 atgagaaaag tatttgtgct ggctgataat atcttttctc ccctgggaaa ggatacggcc
      721 gggaacctgg aagcattgaa acagggacga tccgggatcc ggcggcatca ttccgcggtt
      781 tcaaatgaac ctttttatgc ctctctgctg ttggaggaag ataaaacagg ggacgattac
      841 ttcaccccct tcgaacagat catcttccat tccgtaaaag atgccatggc cggtttggac
      901 ctggaccccg ctaatctgcg gacaggattc attctctcct ccaccaaggg aaatatcagt
      961 gcgatcgaag aggaagggca aggtcccgtt ccggaaagcc gggtgggcct gggcagcagt
     1021 gcggaacgca tcgcaaaacg gctgggaata atgagcgaac ccatcgtggt ttcccatgcc
     1081 tgtatttccg ggctgctggc gatgatcacc ggcatgcgcc tgatccgtac cggtgctttt
     1141 gatgaggtga tcgttaccgg tgccgacctg atcacggcct ttatcctttc ggggtttcag
     1201 tcgttccagg cgatcagccc ggagccctgc cggcccttcg atgccgcaag ggacggcatc
     1261 agcctcggag aaggagcggc caccgtcatc ctttcttcgc ggaaaaagca gggcgccatc
     1321 caactcagcg gaggctccgt gagcaacgat gcaaaccata tttccggccc ctcgcgcacg
     1381 ggagaggaat tatttcttgc ccttaagcgg gcgctgcagc aggcgggtgt ttctcccggc
     1441 gaaattgatt ttatatccgc gcatggaacg gccacccggt acaatgacga tatggaagcc
     1501 aaggcgatca acctggcggg gctcgggtct gtgcccctga acagcctgaa aggtttttac
     1561 ggacatacgc tcggtgccgc gggactgatc gaatccatcg tgtccataca ctcgatgaaa
     1621 gaagacatcc ttttcccgac ccagggattt acaaatcccg gtacggaaaa accggtcaac
     1681 ataataaaag aactggcttc tgcgaaaatc cggaaatgcc tgaaaaccgc ttccggcttc
     1741 ggcggctgca acgccgccat cgtgctggaa aacccgttct gaataagccc gtttggaata
     1801 tctttaaacc cctggaaatt attaaactaa atgaattttt acgctaagga taagaaatcg
     1861 gctatcgaat caaaaggact ggcgcaatgg atcgcattcg gcccggtggt tttccaggta
     1921 gcccgggtgc ttcggaattc cggaatccta aaagtgttga tggaatgccg gagccaggga
     1981 cttaccctgg aagagatccg ggacctggtg aaactgcccc actacggcgt ccggatcctg
     2041 ctggaatccg gcctgggtat cgggctggtc actatccagg acgaaaaata ttttattacc
     2101 aagactggtt tttttatctg caacgatccg ctcacggtgg tcaacatgga ctttgtccac
     2161 gatgtctgct ataaaggatt gttcgccctg gataagagta ttgaaacggg aaaaccggaa
     2221 ggcttgaaag aattcgggaa ctgggacacg atctacgaag gactttcttc ccttcccgcg
     2281 caggtccaga aaagctggtt tgccttcgac catttctttt ccgacattgc tttccccctc
     2341 accctgcccc tggtgtataa cgggagcata aaaaagatcc tcgatatcgg cggcaatacc
     2401 ggaaaatggg ccattgccag cgcgggctat tcaaaggacc tgcagatcac gatcgtggat
     2461 cttcccggcc agctgaaggt ggccaaggaa aaagtaaaac aggccgggct ggaagaacgc
     2521 atcttttttc atccggtgaa tatcctggat gaaagcgctt cttttccgaa aggcttcgat
     2581 gccatctgga tgagccagtt cctggattgt ttttcggaaa cggagatcgt ttcgatcctg
     2641 aaacgctgcc atgccgcact ggacgacgat aaatatgtgc tgatcctgga agcattctgg
     2701 gacgaccagc ggtttgaaac cgcctctttt tgcctgcagc agacctccgt ttattttacg
     2761 gcgctcgcca acggcaacag ccagatgtat aattccaaag tattcatccg ttgtattgaa
     2821 caggcgggtt ttgaaattga agaacgaacg gataatatcg gtctgagcca taccctgctg
     2881 aaatgtaaaa aatcaaaaaa attaaaccaa taaacggaaa cgatcatgcc gatgaattca
     2941 accgatgttc tggtaattgg cgccggtcct gcgggaacgg tggcggcctc tattgtaaat
     3001 aaagccgggt ataaagtgca gattgtggag aagatgaaat tcccgcgctt tgtgatcggc
     3061 gaaagccttc ttccccgctg tatggaagcc cttggcgaag cgggtttcct ggacgccgtg
     3121 aaagaaaaga atttccagga gaagtcgggg gccaaatttg tgaaggacgg aaaaatatgc
     3181 gattataaat ttgccgacca gttcaccagg ggctggcaat ggacctacca ggtgccgcgc
     3241 agcgaattcg acaagaccct ggcagacacg gtggaaaaaa tgggggtgcc ggttcattat
     3301 gaaaccacgg tgaccggcat tgaattcgac ggatcggatt ctgtgaccac cgtgctggac
     3361 aagaacggca atgcgtcccg gatcaaagca aagtttatca tcgacggcag cgggtacgga
     3421 cgcgtaattc cgagattgtt caatatggac agaccatcca acctgcctcc gcgtaaagcc
     3481 ctgttctccc ataccgtgga taaccgcagg gccatgtcgg acgaacccaa ccggatcacc
     3541 attgtcgtgc ataaaccggc cgtctggatc tggatcattc ccttctccaa cgggatcacc
     3601 tccctggggt ttgtcgggga tccggaattt ttccagcaat accccggcac taccgaagat
     3661 cagttgcgca agctcatttc cgtcgaacct tatctggcgg aacgttttga ccaggttgaa
     3721 tttttgtttg aaccgaaaac actgcagtcc tggtcctcca ccaccgaccg ctttttcggc
     3781 gacggattcg tgctcaccgg caacgtgacc gaattcctcg atcctgtttt ttcatccggg
     3841 gtcacgctcg caaccgtttc gagccagatc gcgggaaaac tcgtcgtgga aaaattaaag
     3901 ggccggaata tcgactggga aaacgaatac atgaagccca tgatgcaggg agtggatacc
     3961 ttccgctcct atgtaatggc ctggtatgaa ggaaccctgg ataccatttt ctttgccgac
     4021 gaccaggatg agctgattaa aagccagatc tgttccgtgc tggcgggata tgtgtgggat
     4081 ctggaaaatc cgtatgtaaa aaatcacaag acctccctgc agcgcctggc ctccatcatc
     4141 agctaccggg acagtctcgc cgcaaaaaag aattaatcca tttataaaaa aactattgca
     4201 atccgatcga tttattacgg cttcctgcct gatcagggca aacagcgctt acaaaaacgg
     4261 aaggcaggaa tttcaacggc ccggactgaa tgcttcggat ttcctggatg aactctaccg
     4321 gcatgaacag ctggcctatc ccaagtttca caaaatggac cagctttcca agctgggcct
     4381 cctggcagcc gacattctac tgaaggacca ggatcatcag cagaaatacg cggaggatga
     4441 aaccggtatc gtgctcagca acagcaatgg cagcctggat gtggatctga aatatgcaaa
     4501 gaccatgaaa gcgggcgcca gtccggccct gttcgtctat accctgccca atatcgtgat
     4561 cggtgaaatc gccatccggc accggttcaa gggtgaaaat gcattttttg tatttaaaag
     4621 cttcaatggt aattttatag cggaatatgt gaacggttta tttgaggaca agctggtgaa
     4681 atgctgcatt tgcggatggg tggattttct ggaagaagaa taccgcgccc tgctgatgct
     4741 cgtggagaca tccgcccgcg ggctggcaat cccgtttacg gcagcggata tcaatcaact
     4801 gaacaacgca actcatggat aaattaatgg ccacgctgaa gcgccagatc attgaaaggc
     4861 tgaacctgaa agaccataaa ccggaggata tcggcgacga tcagccatta ttcgtggagg
     4921 ggctgggact ggattccatt gatgccctgg aactgatcgt attgttgcag caggattata
     4981 atatcaaatt gtccaacgca gaagacggac cgaaagtgtt tcattccgtc cgcaccatgg
     5041 cagaatacat taccgaacat tcgccttcga aaatctctcc atgaacacac ccgtatttgt
     5101 agccggcttg ggggtaatat cctcccttgg cccggatgca gccgcttcgc tgcaggcatt
     5161 ccgttccgga gaagcgggca tgaaggagat ccgcggcttg cgcacccggc aaaagggaaa
     5221 attaccggtg gcggaagtgc ccttcagcaa cc
//
LOCUS       DASYCU010000445         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2129221, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000445 DASYCU010000000
VERSION     DASYCU010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129221"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /gene="glgP"
                     /locus_tag="VGM24_08985"
     CDS             <1..1610
                     /gene="glgP"
                     /locus_tag="VGM24_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY4155551.1"
                     /translation="SKKVAYFSMEFAVHQPLKTYSGGLGFLAGSHVRSAYELRQNMIF
                     IGILWKYGYYDQARNQDRTLQVTWVEKQYPFLQDTGIKYQISIHDHPVWVKAWYLAPE
                     TFNGAPLFLLSTDLPENDYVSQTISHRLYDANVATKVAQFILLGVGGAKLVDELKFNP
                     DRYHLNEAHGISAAFYLYQKFNKDVNEVKKRLVFTTHTPEEAGNEKHDIFLCEKMSYF
                     CGLSLQEARDLTGTTDDLFNHSLAALRLAHLANGVSRLHGQVSKALWSKYSGICPIIY
                     ITNAQNWRYWSDKQMYDKLEQKDDYWLDDRKAFLKKRSFELVADQTGRLFDPKVFTIV
                     WARRFAGYKRADLISRDKNRFSELLTNPKYPVQIIWAGKPYPMDYPAINDFNYLVQLS
                     KEHPQVAVMIGYELGLSKRLKQAADLWLNNPRVPREASGTSGMTAAMNGAVNFSTDDG
                     WIPEFIHHGNNGFVIPKADYANMTVPEQDEYDLNQLYEILQNQILPLYYEQRDTWREI
                     IKNGMRDVRFQFDSGRMANEYYEKLYV"
     gene            complement(1611..2333)
                     /locus_tag="VGM24_08990"
     CDS             complement(1611..2333)
                     /locus_tag="VGM24_08990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY4155552.1"
                     /translation="MKRISLFLLISVFSLGSFAQTRKKPASHPVKKAAGPASPAPLKT
                     LTDSLSYSIGMMVANFSKQQGITNINTVQVANAINAEMKGGKPVMDQQQCQQVMMDYV
                     SKQKADKAAGAKKEGSAFLEENKTKPGVISTASGLQYTVLKEGTGPKPLATDKVKCNY
                     EGKLLDGTVFDSSDKQGHPIEFAVSGVIRGWTEALQLMPVGSKWRLFVPSDLAYGDQQ
                     MGPDIKPGSTLIFDVELLEIVK"
     gene            2577..>2923
                     /locus_tag="VGM24_08995"
     CDS             2577..>2923
                     /locus_tag="VGM24_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HEY4155553.1"
                     /translation="MKKLFILLFLAGGLAGVTQAQSLLNNLKKKISSNSSGSSLSSDE
                     IINGLKEALTLGAQKSGDRLSATDGFFKDAAVKILLPPQAQNAEKTLRSMGLGKMVDD
                     AILSMNRAAEDAS"
BASE COUNT          743 a          697 c          731 g          752 t
ORIGIN      
        1 attccaagaa agtagcctat ttttccatgg agttcgctgt tcatcagccg ttgaaaacgt
       61 acagcggcgg actgggattc cttgccggct cccatgtccg gagcgcctac gaactgcgcc
      121 agaacatgat ctttatcggt atcctctgga agtatggata ttacgaccag gcgaggaacc
      181 aggaccgcac attacaggtt acctgggtgg aaaaacagta ccccttcctc caggatacgg
      241 ggattaaata ccagatcagc attcacgatc atccggtttg ggtgaaagcc tggtatctgg
      301 cgcccgaaac ctttaacggc gccccgctat ttttactcag tacggatctg ccggaaaatg
      361 attatgtgtc ccagacgatc agccaccggt tatacgacgc caatgtggcc actaaagtgg
      421 ctcagtttat cttacttgga gtgggcgggg caaagctggt cgatgaatta aaattcaatc
      481 ccgaccggta tcatctgaac gaagcccatg gcatcagcgc agctttttat ctgtatcaga
      541 aattcaataa ggatgtgaat gaggtgaaga agcggctggt ctttaccacc catacgccgg
      601 aagaagccgg aaatgagaag cacgatattt tcctttgcga gaagatgagt tatttctgcg
      661 ggctttccct gcaggaagcg agggatctta ccggcacgac cgatgatctc ttcaatcatt
      721 ctcttgccgc cctgcggctg gcccatctgg ccaatggagt ttcccggttg catggccagg
      781 tctccaaggc cctctggagc aagtatagcg ggatctgtcc catcatttat attaccaatg
      841 cacaaaactg gcggtactgg tccgacaaac agatgtatga caagctggag caaaaggatg
      901 attactggct ggatgaccgg aaggcttttt taaagaaaag atcttttgag ctggttgcag
      961 atcagacggg ccgcttattc gatccaaagg tatttacgat tgtgtgggcc cgccgttttg
     1021 ccggctataa aagagctgat ctcatcagcc gggataagaa ccggttcagc gaattgctga
     1081 ctaaccccaa atatcctgtt cagatcattt gggcaggaaa gccctatccc atggactatc
     1141 ctgccatcaa tgactttaat tacctggtac agctgagcaa ggaacatccc caggtggccg
     1201 tcatgatagg gtatgaactg ggcctttcca agagactcaa acaggcggcg gatttatggt
     1261 tgaacaatcc ccgtgttccc cgggaagcat ccggaaccag cggcatgacc gcggccatga
     1321 acggagcggt taatttttca acggatgacg gctggatacc ggaattcatc catcatggaa
     1381 acaacgggtt cgttattccg aaagctgatt atgcaaatat gaccgttccg gagcaggatg
     1441 aatatgatct caatcaattg tatgagatcc tgcagaatca gatacttccg ctttattatg
     1501 aacagcggga tacctggcgg gagattatca aaaatggcat gcgggacgtc cgctttcaat
     1561 tcgattccgg caggatggca aacgagtatt acgaaaaact ttacgtatag ttatttgact
     1621 atttccagga gttccacatc aaagatgagc gtggaacccg gcttgatgtc cggacccatc
     1681 tgctgatcgc cataagccag atcggatgga acaaagagcc gccatttgct gcccacgggc
     1741 ataagctgaa gggcctccgt ccaacctctg atcacgccgc tcacggcaaa ttcaatggga
     1801 tggccctgct tgtccgaact gtcgaatacc gttccgtcca gcagttttcc ttcataattg
     1861 cacttcactt tgtccgtagc cagtggtttg ggtcctgttc cttctttcag cacggtgtat
     1921 tgtaaacccg atgccgtact gatgacgccc ggttttgttt tattctcttc caggaaagcc
     1981 gaaccttctt ttttcgctcc ggccgccttg tccgctttct gttttgagac ataatccatc
     2041 atcacctgct ggcattgttg ctgatccatg accggttttc cacctttcat ttccgcattt
     2101 atggcattgg ccacctggac ggtgttgata ttggtaatcc cctgctgttt ggaaaaattg
     2161 gcaaccatca ttccgatgga ataactcagg gagtccgtaa gcgtttttag cggggcggga
     2221 gaggccgggc cagccgcttt tttgaccgga tgggaagccg gtttcttcct ggtttgggca
     2281 aaagacccaa gggagaaaac ggaaatcagc aggaataagg aaattctttt catattcatt
     2341 ttttggatcg gcaaaagtaa ggaaactcac cgattgggac taatgatgac cgatttgccc
     2401 gggagcttaa cattttcttt gttttttagg agatactttt ggaagggagc atcgcccatt
     2461 tccctttaaa aggaggaatt caccgatttt ggcaaagtgc tcaaaataac ctttttgtct
     2521 tttcgtttca gatgagccgg gagccagacc ggttcgcctg tttaaaatct atatcaatga
     2581 aaaagctgtt tattttattg ttcttagcag gaggcctggc cggagttacc caggcacagt
     2641 ccttactgaa taatcttaaa aagaagatca gcagcaacag cagcggctct tctttaagtt
     2701 cggatgaaat cattaatggg ttaaaggagg cgttgacgct gggcgcccag aaaagcggcg
     2761 acaggctttc tgcaacggac gggtttttta aagatgccgc cgtaaaaata ttattacctc
     2821 cccaggcaca gaatgcagaa aagacccttc gcagcatggg tctgggcaaa atggttgacg
     2881 atgccatcct gagcatgaac cgggcggcgg aagatgcatc caa
//
LOCUS       DASYCU010000446         6379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1109834, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000446 DASYCU010000000
VERSION     DASYCU010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109834"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VGM24_09000"
     CDS             <1..901
                     /locus_tag="VGM24_09000"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY4155554.1"
                     /translation="LYHPVFYGVKKEDGRVDYDMMEQQAKKEKPRLIICGASAYSRDW
                     DYPRIREIADSVGALVMCDMAHPAGLIAKKLLLSPFGHCHFVTSTTHKTLRGPRGGII
                     LMKKDFDNPFGLKDNKGNIRPMSNLLDMAVFPGTQGGPLEHVIAAKAIAFGEILTREF
                     TDYAMQIIENAQAMGDCFTKKGYHIVSGGTDNHLLLIDLRNKNISGKKAEQALVKADI
                     TVNKNMVPFDDKSAFVTSGIRIGVPAITTRGMKTTDMDEVVDWIDEVLTHAEDEQALM
                     AVRKKVNEKMKAFPLYPEWPDFF"
     gene            921..1340
                     /locus_tag="VGM24_09005"
     CDS             921..1340
                     /locus_tag="VGM24_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HEY4155555.1"
                     /translation="MLQRMQTLWLLFAAVCAFLTIRLSFYSGNLEAAGQPASFQYLNA
                     GFNTWILIITVGLIAASVVSIFLYKKRKIQSRIVIVSLLFSLLNIYLYYKQTLKFTHG
                     DYALTSVLTFLIPVFLFLAARGIYKDEKLVRSLNRLR"
     gene            complement(1406..4246)
                     /gene="uvrA"
                     /locus_tag="VGM24_09010"
     CDS             complement(1406..4246)
                     /gene="uvrA"
                     /locus_tag="VGM24_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY4155556.1"
                     /translation="MAIQTRAKNIPLAKTAVRSPDSIQIKGARVHNLKNISVDFPRNK
                     FIVVTGVSGSGKSSLTIDTLFAEGQRRYAESLSAYARQFMARMDKPDVDYIKGLCPAI
                     AIEQKVTTRTPRSTVGSMTEMYDYLRLLFARVGTTFSPVSGKEVKKNDVQDVVRCIES
                     RKPGDKVLMLVPFKQHTNRDASEELNILLQKGFARLYVNQETVRIEDLLAASAGPSGK
                     PVKGQQPKLPKGNTFLLIDRIVVKDFDEDDLHRLADSVGTAFYEGEGEMLLEINGEKP
                     LAFSNRFELDGIRFEEPVPNLFSFNNPYGACPTCEGFGQVLGIDADLVIPDKRLSVYE
                     GAVAPWKGEKLSAWKDHFIKTSRKYDFPIHKPIMDLSKEQSALLWQGNKEVSGIDDFF
                     AEVEKNLYKVQYRVLLSRYRGRTDCPDCKGYRLRPEALYVKIGGLHIGELCEMPVSKL
                     SDWFREIRFSEFQQKVAKRILIEINNRLITLLDVGLGYLTLNRLANSLSGGESQRIQL
                     TRSLGSNLTNSLYILDEPSIGLHSRDTQRLIVVLKQLRDLGNTVVVVEHDEMMMREAD
                     HIIDMGPLASHLGGEVVAEGDYEAIIQNENSLTGKYLRGDLGIEPPKKPREWKESITV
                     NGARQHNLKNISVKFPLNILCVVSGVSGSGKTTLVKQILYPGLQKLKGEFADKVGLHK
                     SISGDIESISQIELVDQNPIGKSSRSNPVTYIKAYDEIRDLFSKQPLSKLRGFLPRHF
                     SFNVDGGRCDTCKGEGEQVVEMQFLADVHLTCEVCGGKRFKEEVLEVHYHNRNIHDVL
                     EMSVDEALEFFKDERDIIQKIKPLSDVGLGYIKLGQSSDTLSGGEAQRVKLASFLGKG
                     KSLGKILFIFDEPTTGLHFHDIKKLLTSFRALIEQGHSVLVVEHNTDVIRSADWVIDL
                     GPEAGDAGGDLVYEGVPAGLKKQKQSYTGRFL"
     gene            4503..5084
                     /locus_tag="VGM24_09015"
     CDS             4503..5084
                     /locus_tag="VGM24_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4155557.1"
                     /translation="MNSLRTKTDHELIHSFRDGDLQALEALVIRHKDKLYTSILFLVK
                     DKYLAEDIFQDVLIKIIDTIRGGRYTEEGKFLPWAMRIAHNLCVDHFRKVKRTPTIKT
                     SDDRDIFEVINFTEDGADVKIMKRQSHDRVRRMLDLLPEDQREVIILRHYADLSFKEI
                     ASLTNCSINTALGRMRYGLINLRKMMTEKRIAL"
     gene            complement(5213..>6379)
                     /locus_tag="VGM24_09020"
     CDS             complement(5213..>6379)
                     /locus_tag="VGM24_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY4155558.1"
                     /translation="LNNISTNVWLNLAFFVIFVVFAISFFGYFEITIPGSVANSVNNK
                     SGGSSIAGIFFMALTLAIVSFSCTGPILGTLLAGALSNNGGATQLSFGMGGFGLGLAL
                     PFALFALFPGWLTSLPKSGGWLGSVKVVLGFLELAMAVKFISNADLVQQWGIVKREIF
                     IGSWVIIGILLLLYLLGRIRFPHDSPVEKFSRVRIGFIFFFAAATLYLVPGVTNTPAA
                     NLTLISGFPPPLCYSVYKDPVNCEKGVEPLRDYEQALALAKKEHKPLLIDFTGWACVN
                     CRRMEENVWTDNEVKKRMKDSFVVVSLYVDERRLLPAAQQMVYTTKDNASKKIITVGD
                     KWATFQSENFNAVQQPQYAILSPGEKMLTYTKGYTPKPAAFSGWLDCGLRAFRK"
BASE COUNT         1614 a         1693 c         1511 g         1561 t
ORIGIN      
        1 gctctatcat ccggtttttt acggagtgaa gaaggaggat ggccgggtgg attatgatat
       61 gatggaacag caggcaaaaa aagaaaagcc caggttgatc atctgcggcg cttccgctta
      121 cagccgcgat tgggattacc cccggatccg ggagatcgcc gattcggtcg gcgcgctcgt
      181 gatgtgcgat atggctcatc ccgcgggcct cattgctaaa aaattattgc tttctccctt
      241 cgggcattgc cattttgtaa cgtccaccac ccacaaaacc ctgcggggcc cgagaggtgg
      301 tatcattctc atgaaaaaag atttcgacaa tcctttcgga ttaaaagata acaagggaaa
      361 catccggccc atgagcaatc tcctggatat ggctgttttt ccgggcaccc agggcggacc
      421 gctcgaacac gtgatcgctg ccaaagcaat cgctttcggt gaaatactca cccgggagtt
      481 cacggattac gcgatgcaga taatcgaaaa cgcacaggcc atgggcgact gctttactaa
      541 aaaaggatac catatcgttt cgggcggaac ggataatcac ctgctcctga ttgaccttcg
      601 gaataaaaac atcagcggga aaaaagcaga acaggcattg gtaaaagcag atatcacggt
      661 caataagaac atggttccct tcgacgataa gagcgcgttc gtcacgtccg ggatccgcat
      721 cggcgttccg gcaatcacca cgcgcggcat gaagacaacg gatatggatg aagtggtgga
      781 ctggatcgat gaagtattga cccacgccga agatgaacag gccttgatgg ccgtgcgcaa
      841 aaaagtgaat gagaaaatga aggcttttcc gttgtatccc gagtggcccg atttctttta
      901 aagaactaaa accgatttat atgctccagc gtatgcaaac cctttggctg ctctttgcgg
      961 ccgtttgcgc tttccttacc ataagacttt ctttttacag cggtaacctg gaagcggccg
     1021 gacagccggc cagttttcaa tacctgaacg ccggtttcaa tacctggatc ctgatcatca
     1081 ccgtcggact gatcgcggct tccgtggtca gtattttttt atacaagaaa cggaaaattc
     1141 aatcccgcat cgttatagtg tcccttcttt tttccctgct gaatatttac ctgtattaca
     1201 aacaaacgct gaaattcacc catggcgatt acgcgctgac ctccgtattg accttcctga
     1261 tcccggtctt tctttttctt gctgcccggg gaatctacaa agatgaaaag ctggtgagaa
     1321 gcctgaaccg gctgcgctga atccgtattt tcccgccgct ctgatatccg cgaagggagg
     1381 caggataaaa ttcctgcagc aaaaatcaca gaaatctccc ggtatagctc tgcttctgct
     1441 ttttcagacc ggcaggaacg ccttcataca cgagatctcc gcccgcatct cccgcttcgg
     1501 gtccgaggtc aatgacccag tccgcggaac ggatcacatc ggtattgtgt tccaccacca
     1561 gcacggaatg cccctgttca atcagggcgc ggaaggaggt gagcagtttc tttatatcgt
     1621 gaaaatgaag tccggtcgtc ggctcatcaa aaatgaaaag gatcttcccc aggctttttc
     1681 cctttccgag aaaagaggcg agtttgaccc gttgcgcttc cccgccggat aaagtatccg
     1741 aagactggcc cagtttgata tatcccaatc ccacatcgct cagcggcttt attttctgaa
     1801 tgatatccct ttcatcctta aaaaattcca acgcctcatc cacactcatt tccagcacat
     1861 catgaatgtt ccggttatga taatgcactt ccagcacttc ctccttaaac cgttttccgc
     1921 cgcaaacctc gcaggtcagg tggacatcgg ccagaaactg catttccacc acctgttccc
     1981 cttctccctt gcaggtatcg cagcggcccc cgtccacgtt aaaggaaaaa tgccggggaa
     2041 gaaaaccccg gagtttactc agcggctgtt tggaaaaaag atcccggatc tcatcatatg
     2101 ctttgatata ggtcaccgga ttgctccggg acgacttacc gatgggattc tgatccacca
     2161 gctcgatctg gctgatagat tcgatatccc cgctgatgga tttatgcaaa cctactttat
     2221 ccgcaaattc tcctttgagc ttctgcaggc cgggataaag gatctgtttt accagggttg
     2281 ttttaccact gccgctcacg ccgctgacca cacagagtat gttaagcgga aatttcaccg
     2341 atatattttt cagattatgc tggcgtgccc cgtttacggt aatggactcc ttccattccc
     2401 tcggcttttt gggtggctcg atccccaggt ctccccgcag gtatttgccg gtgaggctgt
     2461 tttcattctg aataatcgcc tcataatccc cttccgccac cacttccccg cccaggtggc
     2521 tggccagcgg gcccatatca ataatatggt cggcctcgcg catcatcatt tcgtcgtgct
     2581 ccaccaccac caccgtattt ccgaggtcgc gcaactgttt cagcaccaca atcagccgtt
     2641 gcgtatcgcg ggaatgaagc ccgatggacg gttcatccag tatatataat gaattggtga
     2701 gattgcttcc gagggaacgg gtcagctgga tccgctgact ttcccccccg ctcaacgaat
     2761 tcgcgagccg gttcagggta agataaccca gacctacgtc gagcagggtg atcagccggt
     2821 tattgatctc aatcaggatg cgtttggcca ctttttgctg aaattcggaa aagcggatct
     2881 cccggaacca atctgacagt ttgctcaccg gcatttcaca gagttccccg atatgaagtc
     2941 ccccgatctt tacgtacagg gcttcgggac ggagccggta tcctttacaa tccgggcagt
     3001 ccgtccggcc ccggtagcgc gaaagcaaaa cccggtactg aaccttatac aggttctttt
     3061 ccacctcggc aaagaaatcg tcgatgccgc tgacctcctt gttcccctgc cataggagcg
     3121 ctgactgctc tttgctaagg tccatgatgg gtttatggat cggaaaatca tatttgcggg
     3181 aagtcttgat gaagtgatct ttccaggcac tcagcttctc gcccttccag ggagccacag
     3241 ccccttcata gacgctcagc cgcttatccg gaatcaccag gtcggcatca atacccagca
     3301 cctggccaaa gccttcgcag gtcgggcagg cgccatacgg gttgttgaaa gaaaaaagat
     3361 tgggcacggg ctcttcaaaa cggataccgt ccaattcgaa ccggttgctg aaagcgagcg
     3421 gcttttcccc gttgatctcc agcagcattt ctccctctcc ttcataaaaa gccgttccga
     3481 ccgaatccgc cagccggtgc aaatcgtcct catcaaaatc cttaactacg atccggtcga
     3541 tcagcaggaa ggtattcccc ttgggaagct tgggttgctg gcctttgacg ggttttccgg
     3601 aagggccggc gctcgcggcc aggaggtcct cgatccggac cgtttcctgg ttcacgtata
     3661 atctcgcaaa tcctttctgt aagagaatat tcagttcttc ggaggcatcc cggttggtat
     3721 gctgtttaaa cggaaccagc atcagcacct tatcgccggg tttgcgggat tcgatgcacc
     3781 ggaccacatc ctggacatca ttctttttta cctcctttcc ggaaaccggg gaaaaagtag
     3841 tccccacgcg ggcaaaaagc aggcggagat aatcatacat ttccgtcatg ctgcccaccg
     3901 tggagcgggg agtgcgggtg gtcactttct gctctatggc gatggccggg cataagccct
     3961 tgatatagtc cacgtcgggt ttgtccatcc gcgccatgaa ctgccgcgca taggcgctca
     4021 ggctctcggc ataacggcgc tggccctccg caaacagggt gtcgatcgtc aacgaagatt
     4081 tgcccgatcc agatacaccg gtgaccacaa taaatttatt gcgtggaaaa tcaaccgaga
     4141 tgttcttcag attatgtacc cttgcacctt taatttgaat cgaatccggt gaccggacag
     4201 ctgtcttcgc gagaggaata tttttcgccc tggtttgaat tgccataaat gagaacaaca
     4261 aatatattgg aactgaaaca attagatagg ttttcccaat tcaggaaata ccggtgagat
     4321 ctataagaac aaataatttt cccaaaatgt tgttttttcc aaaaattgta tatctttaac
     4381 gctgattaaa tttttaacat cacaataaaa taaattttat cgagtaagca cttctacacc
     4441 gaaaagccaa gcgttcaaaa atccaacata tatcctatca cctttaaaaa ccgtagaagt
     4501 ttatgaattc gctacgtacg aaaaccgatc atgaattaat ccattcgttt cgcgacggcg
     4561 acttacaggc cttagaggct ctcgtaatcc gccacaagga caagctttac acttccattc
     4621 tcttcctcgt taaggacaag tatctggccg aggacatttt ccaggacgtt ctgattaaga
     4681 ttatcgacac catccgtggt ggccggtaca cagaagaagg caagttcctt ccctgggcaa
     4741 tgcgcattgc tcacaacctc tgcgtggacc atttccgcaa ggtgaaaagg acccccacca
     4801 tcaagaccag tgatgaccgg gacatttttg aagtcatcaa tttcacagaa gacggcgctg
     4861 acgtaaaaat catgaaaagg caaagccacg accgtgtacg acggatgctg gacctgcttc
     4921 ctgaagatca gcgcgaagtg atcattctgc gtcattatgc cgacctgagt tttaaagaga
     4981 tcgcctcttt aaccaactgc agtatcaata cggccctggg ccgcatgcgg tacgggctga
     5041 tcaacctgcg aaaaatgatg actgaaaaaa gaatcgcctt ataagctaaa ttttacaccc
     5101 ttttttcaaa aattcctgtc ttccggtgtc cggcattttc aataaaagaa aatcttccac
     5161 accggaagtt tttttttgta tggaatgaag gatctttccc gtgcgggagg ccttatttac
     5221 ggaaggccct caggccgcag tccagccagc cggaaaaagc ggccggtttg ggtgtatacc
     5281 ccttggtata ggtaagcatt ttttcaccgg gactgagaat ggcatactgg ggctgctgaa
     5341 cggcattgaa attttccgat tgaaaggttg cccatttatc tccaaccgta atgatctttt
     5401 tgcttgcgtt atctttcgtg gtataaacca tttgttgcgc ggcaggtaaa agtctgcgct
     5461 cgtccacata aagggaaacg accacaaagg aatccttcat ccgttttttt acttcattgt
     5521 cggtccatac attttcctcc atgcgccggc agttgacaca ggcccacccg gtgaaatcga
     5581 tcagcagggg tttgtgttct tttttagcca gggccagcgc ctgttcgtaa tccctcagcg
     5641 gctccactcc cttctcacag tttaccgggt ctttataaac ggaataacac aacggtgggg
     5701 gaaagccgct gatcagggtg agattggcag ccggcgtatt ggtcactccc ggaacgagat
     5761 aaagcgtagc ggctgcaaag aagaagataa atccaatccg tacccggctg aacttttcta
     5821 ccggactgtc gtgcggaaaa cggatcctgc ccagcaggta taaaagcagg agtattccga
     5881 taatcaccca ggacccgatg aaaatttccc gcttaacaat tccccattgc tgcacgaggt
     5941 cggcattgga aatgaatttc accgccatcg ccagttcgag aaaaccgagc accactttta
     6001 cggaaccaag ccatccgccg gatttgggaa gggaggttag ccatcccgga aataaggcaa
     6061 agagggcaaa gggcagggcc aggcccaatc caaatccgcc cattccaaag ctaagctggg
     6121 tagctccgcc gttgttggac agggccccgg ccagcagcgt acccaggatc gggcccgtgc
     6181 aggagaaaga aacaatggcc agggtaaggg ccataaaaaa aatgccggcg atactgctgc
     6241 cgcccgattt gttatttacg gaattggcca cgctgccggg aatcgtaatt tcaaaatacc
     6301 cgaaaaagga gatcgcaaat acgacaaaga tcacaaagaa ggccaggttg agccagacat
     6361 tggtggagat attattcaa
//
LOCUS       DASYCU010000447         3991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3575858, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000447 DASYCU010000000
VERSION     DASYCU010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575858"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1457)
                     /locus_tag="VGM24_09025"
     CDS             complement(39..1457)
                     /locus_tag="VGM24_09025"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY4155559.1"
                     /translation="MPARNEYKFFEAVEKSFDKATPFTSWDPGILEQIKQCNAVYRMF
                     FPVKIGDKIEVIKAYRVQHSHHKTPCKGGIRFALSVNLDEVMALAALMTFKCAIVNVP
                     FGGAKGGIAIDPKKYSAYDLEKITRRYTSELIKKNFIGPGTDVPAPDYGTGEREMAWI
                     LDTYLSMRPGEIDALGCVTGKPVTQGGVRGRREATGLGVFYGIREVCLLPDLMSRLGM
                     KTGVQGKTVVVQGLGNVGYHAAKFFREGGAKVIALAEFEGAITHPEGLNEEEVFQHRK
                     KTGSILNFPGATNIPKSVDALELQCDILIPAALENVINGNNAPRIKARIIGEAANGPL
                     TPEADEVFIRKGVLVVPDMYLNAGGVTVSYFEWLKNLSHVRYGRMEKRFTENLNTHIL
                     SQLEELTGKNVGSKEREFIMHGPEEADLVYSGLEETMITATHDIMNCWKANPKIPDMR
                     TAAYVVAINKVGTAYAELGIFP"
     gene            complement(1502..1735)
                     /locus_tag="VGM24_09030"
     CDS             complement(1502..1735)
                     /locus_tag="VGM24_09030"
                     /inference="COORDINATES: protein motif:HMM:NF040113.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HEY4155560.1"
                     /translation="MPKPFLRLFFSVTGMLIVFFVRAQDSLRNEKPQMADAMRSNGKI
                     YVVVAVLLVILAGLFLYLFQTDRKLTRLEQKLK"
     gene            complement(1728..2399)
                     /gene="ccsA"
                     /locus_tag="VGM24_09035"
     CDS             complement(1728..2399)
                     /gene="ccsA"
                     /locus_tag="VGM24_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY4155561.1"
                     /translation="MLRKNWWKILSVLMLLYTFTAGFLGGVPAKPILNETIRNLYFHV
                     AMWFGMMIFFIISVVYSLKYLRNNNPVHDVYAVEFARTGIIFGTLGLLTGSVWARYAW
                     GAFWSNDPKQIGAAVALLIYLAYFVLRNSLLDLDKAARIGAVYNIFAFAMLFPTIWIM
                     PRLVESLHPGGMGNPALDTKDIDSRMRVIFWFGAIPGWSLLGGWICTLRIRMKLLSLN
                     HESNA"
     gene            complement(2475..3134)
                     /locus_tag="VGM24_09040"
     CDS             complement(2475..3134)
                     /locus_tag="VGM24_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY4155562.1"
                     /translation="MKAFLFILTLFKKEVLLEFRQQYSFYGVLLYVASTIFVLYLAMG
                     QPENTVWNGLFWMMQLFICVNAVAKSFLQESQGRQLYFYSVCHPMHFMLAKMIFNAVL
                     MLLMSMVSLLLFRLFLGSPLSHPVLFIGISCFGAISISFVFSFLAAIAARAQQNAALM
                     AILGFPLIIPELMLLMRMSNIAFADVYQSGLLSILLLLFCFDVMVIVLSLILFPFLWK
                     D"
     gene            complement(3150..3728)
                     /locus_tag="VGM24_09045"
     CDS             complement(3150..3728)
                     /locus_tag="VGM24_09045"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY4155563.1"
                     /translation="MFIEPAIKFERRGWIEVICGSMFSGKTEELIRRLRRARIANLRV
                     AVFKPAVDKRYHEENIVSHDETSLLSLPVPDSSFILQQAGSVEVVGIDEAQFFDEQIP
                     AVCESLALQGIRVIIAGLDMDYTGRPFGQMPELLAKAEYITKLHAICMQCGNIASYSY
                     RKGGSSAQFELGNKDLYEPRCRYCFEKGELLK"
BASE COUNT         1070 a         1056 c          916 g          949 t
ORIGIN      
        1 tgatggtgct gccttcctat gatgaataag gaagatccct atgggaaaat tcccagttcc
       61 gcatacgccg tcccgacctt attaatcgcc accacgtagg cggcggtgcg catatcggga
      121 atttttggat tggccttcca gcagttcatg atatcatggg tggccgtgat catggtttcc
      181 tccagtccgc tgtagaccag gtccgcctct tccggcccat gcatgataaa ttcccgttcc
      241 tttgatccga cattcttgcc ggtgagttct tccagctggc tgaggatatg ggtattcagg
      301 ttttcagtaa accttttttc cattctgccg taccggacat gactgagatt tttcagccat
      361 tcaaaataag atacggttac cccgccggca ttcaggtaca tgtccggcac caccagcact
      421 cctttccgga taaatacctc gtcggcttcc ggcgtcaggg gaccgtttgc cgcctccccg
      481 atgatccgcg ctttgatccg cggtgcatta ttcccgttga tcacattctc cagcgcagcc
      541 gggatcagga tatcgcattg gagttccagc gcgtccaccg acttcggaat attggtggcg
      601 cccggaaaat tcaaaataga tcctgttttt ttgcggtgtt gaaatacctc ttcttcattc
      661 agcccctccg gatgggtgat ggctccttca aattcagcca gagcgatcac tttggcaccg
      721 ccttcacgaa aaaatttagc ggcatgataa cccacatttc ctaggccctg gacaacgacc
      781 gtctttccct gcactcccgt tttcattccc agccggctca tcaggtccgg cagaagacat
      841 acttcccgga ttccgtaaaa cacacccaga cccgtagctt cccttcttcc tcttacgcct
      901 ccctgagtga ccggtttccc ggtaacgcag cccagcgcat ctatttctcc cgggcgcata
      961 cttagataag tatccagtat ccacgccatt tcccgctcac cggttccgta atcgggagcc
     1021 ggaacgtcgg tacccggccc gataaaattc tttttgatga gttcagacgt gtagcggcgc
     1081 gtaatttttt ccaaatcata cgcggaatat tttttcggat cgatggcgat ccctcctttg
     1141 gcgccgccga acggcacgtt cacgatcgca catttaaagg tcatcagggc tgccagcgcc
     1201 atcacctcgt cgaggttcac ggaaagggca aaacggattc cgcctttgca gggtgttttg
     1261 tgatgggaat gctgtacgcg gtaggctttg atcacttcaa tcttatcgcc gatcttcacc
     1321 gggaagaaca tgcggtaaac ggcattgcac tgttttatct gttccagtat gccggggtcc
     1381 cagctggtaa aaggagtcgc tttgtcaaaa cttttttcta ctgcttcaaa gaatttatat
     1441 tcgttacgcg caggcataaa aagtatattt taatgatagg atgatggttc ggggtgatcg
     1501 gtcatttgag tttttgttcg agccgggtca gtttccggtc cgtttggaaa agatacagaa
     1561 atagtcccgc cagaatgacc agcaacacgg caaccacaac ataaattttc ccattggaac
     1621 gcatggcatc ggccatctgg ggcttttcgt ttctgaggct gtcctgggcc cgcacaaaaa
     1681 atactatgag cattccggta acgctgaaga ataaacgcag aaatggttta ggcattgctc
     1741 tcgtgattta gggataaaag cttcatacgg atacgcagcg tgcagatcca gccgcccagc
     1801 agggaccagc ccgggatggc gccaaaccag aaaatgaccc gcatccggct atcgatgtct
     1861 ttggtatcca gggccggatt ccccatgccc ccggggtgca ggctttccac cagcctcggc
     1921 atgatccaga tggtcggaaa aagcatggca aaggcaaata tgttatatac cgcaccgatc
     1981 cgggccgcct tgtccaggtc cagcagggaa ttccgaagta caaaataggc gagatagatc
     2041 agcagggcca cggcggcccc gatctgtttg gggtcattgc tccagaaggc gccccaggca
     2101 taccgggccc agacggaacc cgtgagcaga cccagcgtac cgaaaataat cccggtccgg
     2161 gcaaattcca cggcatacac atcgtgaacc ggattgttat tccgcagata tttcagggaa
     2221 taaaccaccg aaataataaa gaagatcatc atcccgaacc acatggctac atggaaatag
     2281 aggttccgga tggtttcgtt gagaatgggt tttgccggca cacctcctaa aaatcctgcc
     2341 gtgaatgtgt agagcagcat taaaacactt aaaatcttcc accagttctt acgaagcata
     2401 ttatttactt aattggcagc tttcatccgg aggcaaaagt aagacaagcc actcaaaccc
     2461 gaaaaaaacc ttcctcagtc cttccacaag aatggaaaaa ggatcaacga aagaacgatc
     2521 accattacat caaaacaaaa aagcaggaga agaatggaca acaatccgct ttgatacaca
     2581 tcagcgaatg caatattcga catccgcatc agcagcatca gttccggaat aatgagcgga
     2641 aaccccagga tggccatcag cgccgcgttc tgctgggccc ttgcggcaat ggcggccaga
     2701 aaactgaaaa caaaactgat gctgatggcc ccaaaacacg aaatgccgat aaaaagaacc
     2761 ggatggctca gcggacttcc cagaaacagg cggaacaaca ggagactgac catgctcatc
     2821 agcaacataa gcactgcatt gaagatcatt tttgccagca tgaaatgcat gggatggcaa
     2881 accgaataaa agtacagttg ccggccctgg ctttcctgca gaaaactctt ggccacggca
     2941 ttgacacaaa tgaacagttg catcatccag aacaacccgt tccagaccgt attttccggc
     3001 tggcccatcg ccaggtacaa aacaaaaata gtagaagcca catagagaag aacaccgtaa
     3061 aaactgtatt gctgacggaa ctcgagtaaa acttcctttt taaaaagcgt aagaataaac
     3121 aggaaagcct tcaattgatt tctttaaatt tattttaaca attctccttt ttcaaaacaa
     3181 taacggcatc ggggctcgta aaggtccttg ttccccagct caaattgtgc agaacttccc
     3241 cccttccggt aagaataact ggcaatattc ccacattgca tacaaatggc atgtaacttg
     3301 gtgatatatt ccgcttttgc cagcagttcg ggcatttgcc cgaaaggtct gccggtataa
     3361 tccatatcca gtccggcaat gattacccgg attccctgaa gggccaggct ttcacaaaca
     3421 gccggaatct gttcgtcaaa aaactgcgct tcgtcaatac cgaccacctc cacagatcct
     3481 gcctgctgca ggataaagga ggaatcgggt acgggaaggg aaagcaggct ggtttcatca
     3541 tgagatacga tattctcctc atgatacctt ttgtcaacgg ccggtttaaa aacagccacg
     3601 cggagattgg caatccgggc gcgcctcagt ctgcggatga gttcttccgt tttcccggaa
     3661 aacatcgaac cgcagatcac ttcgatccat ccgcggcgct caaatttgat ggcgggttca
     3721 ataaacatcg taccggaatc cagtttggtt aaatatttgc attaatttta ggtacccgtt
     3781 aataatcaca aaaatctgtc ttgtccgaaa ctatacgatg gaaagattac aacatctgat
     3841 cagcaagtta aacgaacaat ccgaacaaaa ggacagcccc cttcaaatgc tggttaccct
     3901 taaacagatt gaagcggaac tgacccggat gcatacggtg accgccaccg gaagaaaaaa
     3961 tccgggggtt tcggttgtca tgccttccct g
//
LOCUS       DASYCU010000448         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_821619, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000448 DASYCU010000000
VERSION     DASYCU010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821619"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VGM24_09050"
     CDS             complement(<1..748)
                     /locus_tag="VGM24_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY4155564.1"
                     /translation="MKFFLFGLLICGFTATAQVNIIPQPVSVKMPRKMGDFSFSRSTK
                     IVLKGGGLENSVQFFNDYLKEYYGFSLETTMEAKGNNNLILKDERLGDSIPGAYHLSV
                     NQKGISIGGDNETGVFYGIQTLIQLLPMPDGTKPLSIPYVQIDDKPRFAYRGLMLDVG
                     RHFMPVPFVKKFIDYLALHKMNEFHWHLTEDQGWRIEIKKYPRLTEVGAWRDGTVIGG
                     RPWKGNDNIRYGGFYTQDQIRDVVAYASKRY"
     gene            851..1411
                     /locus_tag="VGM24_09055"
     CDS             851..1411
                     /locus_tag="VGM24_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEY4155565.1"
                     /translation="MLSFISQTWPWYVSGLAIALIMTLLLFFGKSFGFSSNLSNICSM
                     LGAGKRVSFFNFDWKKEQWNLWFLVGSVIGGFISVHWLQSGEPLKLSAATVADLKKLH
                     IPFDGRLEPSVIFNWDFLRTGKGLFLFLGGGFLIGFGTRYAGGCTSGHAISGLSNLQV
                     PSLLAVVGFFIGGLIMTHLLFPVIFS"
     gene            1495..1908
                     /locus_tag="VGM24_09060"
     CDS             1495..1908
                     /locus_tag="VGM24_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEY4155566.1"
                     /translation="MKAVKYIFAGIVFGIILTKSEAVSWYRIQEMFRFQSFFMYGIIG
                     SAVIPGIIIIALIKKFRLKDISGIPIRFEPKDKRWKKYVFGGTIFGLGWALTGACPGP
                     VFVLLGYGYSVMLLVIAGALLGTFTYGVFRDRLPQ"
     gene            1919..>2798
                     /locus_tag="VGM24_09065"
     CDS             1919..>2798
                     /locus_tag="VGM24_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY4155567.1"
                     /translation="MKFYLACFLSVLLLSSHTAEKETSSWIRINQLGYTPSGIKVAVW
                     CSREMEQIKNFSVMDSASGKRLFKGRTGRAFGAYGPFAQTYRLNFSSFNKPGTYCVMA
                     GGVRSAYFRIGTDVYEGSADFCLRYMRQQRSGFNPFLKDSCHTHDGYTLYGPMPDSTR
                     VDVVGGWHDATDYLQYSATSANATYHLLMAYRDFLEVFGDEKEANGLDGKNGIPDVLD
                     EAKWGLDWLLKMHPRPDWMFNQIADDRDHMGMRLPKEDSQYGRGYERPVYFVSGEPQQ
                     RGKFMNHTTGTSSTAGK"
BASE COUNT          693 a          627 c          701 g          777 t
ORIGIN      
        1 catatcgttt ggatgcgtaa gccaccacat ccctgatctg gtcctgcgta taaaagccgc
       61 cgtaacgaat attgtcattg cctttccagg gccgtccgcc gattaccgtg ccgtcgcgcc
      121 aggcgcctac ttcagtcaga cgcggatatt ttttaatctc gatccgccag ccctggtctt
      181 ccgtcagatg ccagtggaat tcgttcattt tatgcagggc gaggtaatcg atgaattttt
      241 ttacaaaagg gaccggcata aaatgacggc caacatccag catcagtccg cggtaagcaa
      301 agcggggttt gtcatcaatc tgaacataag gtatggacag cggctttgtt ccatccggca
      361 tgggcaataa ctggatcagc gtttgaatgc catagaaaac cccggtttca ttgtcgcctc
      421 cgatggaaat ccctttttga ttaacggaaa gatggtaggc ccccggaatg gaatctccca
      481 ggcgttcatc cttcaggatc aggttgttat ttccttttgc ttccatcgtg gtttccaagc
      541 tgaaaccata atattccttt aaataatcat tgaagaactg aacggaattt tcaagaccgc
      601 cgcccttcag gacaattttg gtggaacggc tgaaggagaa atcccccatc ttccggggca
      661 tttttacgga aacgggttgc ggaataatat ttacctgggc agtggcagta aagccacaaa
      721 tcaaaaggcc aaagagaaaa aatttcatgg caatcgcttt taagtactgc aaacttattc
      781 tattccggtt aggcgagcat agtttttgta acttagtacg gatctttttt cattcatcaa
      841 tttgttccgc atgctttctt ttatcagcca aacctggccc tggtatgttt ccggactggc
      901 catcgccctg atcatgacct tgctattgtt tttcgggaag tcctttggat tttcgtcgaa
      961 cctgagcaat atctgttcta tgctgggagc cggcaagcgg gtctcttttt ttaatttcga
     1021 ctggaagaaa gaacaatgga acctgtggtt tctggtgggt tctgtaattg gcggatttat
     1081 ctctgttcac tggctgcaat cgggggaacc cttaaagttg tccgcagcaa cggtggctga
     1141 cctgaaaaaa ctgcatatcc catttgacgg ccggctggag ccatcggtca ttttcaactg
     1201 ggactttctc cggaccggga aagggctctt tttatttctg ggcggtggct ttttaatcgg
     1261 gtttgggaca aggtatgccg gcggatgtac ttcgggtcat gccatatccg gactttcaaa
     1321 ccttcaggtc ccgtctttac ttgcggtggt cggatttttt atcgggggac tgattatgac
     1381 ccatctttta tttccggtca tttttagtta ggacgccatc cgtttattaa aagattcgca
     1441 tcgggataaa aaacaaaatg ggaaacggac aaattaatga ataacaaata aatagtgaaa
     1501 gcagtaaagt atatattcgc cggaatcgta tttgggatca tcctgaccaa atcggaagca
     1561 gtgtcctggt atcgtatcca ggaaatgttc cgctttcagt catttttcat gtatggaata
     1621 attggcagtg ccgtaattcc aggcattatt atcatagcgc tcatcaaaaa attcagatta
     1681 aaagatattt cgggaatacc catccggttc gagccgaagg ataaacgttg gaaaaaatac
     1741 gtgttcggag ggaccatatt cggattgggc tgggcgctga ccggtgcctg tccgggacct
     1801 gtgttcgtgt tgctgggcta tggctacagc gtaatgctgc tggtgattgc cggcgcttta
     1861 ttgggaacat tcacctacgg agtattccgg gatcgtcttc cgcaatagca atttatttat
     1921 gaaattttat ctggcctgtt ttttatctgt tctgcttctt tcttcccata ccgcagaaaa
     1981 ggagacatcc tcctggatca ggataaacca gttgggttat acgccttcag gaataaaggt
     2041 ggctgtctgg tgcagccggg aaatggaaca aataaaaaat ttttcggtga tggattcggc
     2101 ttctggcaag cgcctgttca aaggcaggac cggccgggct tttggcgcct atggtccttt
     2161 tgcgcagacc taccggttaa acttcagctc atttaataaa ccgggcactt actgcgttat
     2221 ggcaggcgga gtccggtcag cttatttccg gatcggaact gacgtttatg aaggatccgc
     2281 cgatttctgc ctccggtaca tgcgtcagca gcgaagcgga tttaatcctt ttttaaaaga
     2341 cagttgtcat acccacgatg gatatacctt atatggtcca atgccggaca gtacgcgggt
     2401 agatgtggtg ggcggctggc atgatgcaac ggattacctg caatattccg ctacttcggc
     2461 taacgcaacc tatcatttac tcatggcata ccgtgatttt ctggaagttt tcggagatga
     2521 gaaggaagcc aacggactgg atggcaaaaa cggaatcccg gatgtgctgg atgaagctaa
     2581 atgggggctg gactggctgt tgaaaatgca tcccaggccg gattggatgt tcaaccagat
     2641 agccgacgac cgggatcaca tgggaatgag gctgccaaaa gaagacagcc agtatggccg
     2701 gggttacgaa agaccagttt atttcgtcag tggcgaaccc cagcagcggg gaaagttcat
     2761 gaatcacacg accggcactt cttccaccgc cggaaaat
//
LOCUS       DASYCU010000449         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_242774, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000449 DASYCU010000000
VERSION     DASYCU010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242774"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGM24_09070"
     CDS             complement(<1..398)
                     /locus_tag="VGM24_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4155568.1"
                     /translation="MSRQVLFIQGGGNGGYEVDASLAASLRAALGKGYDIHYPRMQTD
                     EAKPDFGWPQQIGREISAVKGDVILVAHSLGASLLLKYLSENMAEKEIAGIFLLSTPF
                     WSGDEDWQKGLKLPENFAGKLPKEVPVFFY"
     gene            complement(476..1834)
                     /locus_tag="VGM24_09075"
     CDS             complement(476..1834)
                     /locus_tag="VGM24_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4155569.1"
                     /translation="MKSSNANPTFFFCQALVISSLMLATTFRVHAQNLVVNSSFTGGS
                     STGWSTGSSLEINPQSVYGGTSSTDYATELDVERTINQKVCILPGLSYTLTYQASRRT
                     GGGTPANPGLQVMVTGTASGTNYVNKIQAYTNTNWSAHDNSFTFSVPSGSADKQVNIQ
                     FLSNNNSGTYGVLVWDIELAPAATNALSVAGPVSVSLSTPAAFAVTNAPSGALYKWSF
                     PGAGTSTSARARPSGISWSSLGVKTVSTALSNGTCTMASYSKAVTVVVVLPVQWTNFE
                     GSIINHNAHLTWIAREESNGRYFIIQRSVNSADFDSVGVIASLNTSSAQTYSFTDGNT
                     PAGRVSYRVVHVDLDNNIEYSSVLSLKNDLIPVTGVMRIFPNPAVTTLDCSISSETES
                     QATIRVFNLSGLLMITTELHLTIGSNRTTLNISSLRPGVYFLNTINAKTGARAVQAFV
                     KE"
     gene            complement(2176..3108)
                     /locus_tag="VGM24_09080"
     CDS             complement(2176..3108)
                     /locus_tag="VGM24_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylase"
                     /protein_id="HEY4155570.1"
                     /translation="MTRILFMRKQVLCVLTGFLFIQCHTDHTKKEFPDELVHFVSYRH
                     NPIFSGTDTSTWDRHIRERGWILKEGSEYHMWYCGYTNIDSEKHLGYATSPDGISWKR
                     YPANPIYAAGWAEDVCVVKSDGIYFLFSEGRGDTSHLLTSPDRIHWREQGNLDIRQTN
                     GNPIQPGPYGTPAVWKSDSAWYLFYERDDHGIWLAVSTDLKTWKNVQDDPVIAMGPDA
                     YDKFAVAMDQVIRYNGLYYGYYHASAFKDWKEWSTNVAVSNDLIHWKKYPGNPVVAEN
                     KSSGILVNDGRRYRLYTMHDKVNLHFPVTDPAAQ"
     gene            complement(3147..>3503)
                     /locus_tag="VGM24_09085"
     CDS             complement(3147..>3503)
                     /locus_tag="VGM24_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY4155571.1"
                     /translation="WLPPYGFLCTVHEMKAEVNGTYKMSFQNFTTGNSHSFGGKYLEI
                     KPNAFLKYTDKFDDPNLPGEMITSVSLQKVSVGTEIKISQENIPAVIPAEMCYLGWQE
                     SLEKLKKLVEPEIPDM"
BASE COUNT          883 a          841 c          879 g          900 t
ORIGIN      
        1 tggtagaaaa ataccggcac ttctttgggc agcttaccgg caaaattttc cggcagctta
       61 agtccttttt gccaatcttc atcgccgctc caaaacgggg tggagagaag aaaaatccct
      121 gcgatctctt tttcagccat attttcggaa agatatttca acagcaggga agcgccgaga
      181 gaatgcgcca ctaaaatgac atcgcctttg actgcagaaa tttccctgcc gatctgctga
      241 ggccatccga aatccggctt ggcttcatcg gtctgcattc ggggataatg gatatcgtaa
      301 cccttcccca atgctgcccg gagggaggct gccaggcttg catcaacctc ataaccgcca
      361 ttacctcctc cctgaataaa gagtacctgc ctgctcatag ggaatgctgt atttcatttt
      421 acaatacccg ctaatacgca aaattatgat aaactctatt tggctgtgca aatgatcatt
      481 cttttacaaa agcctgcacg gcccgggcgc cggtctttgc gttgatggta ttcaaaaagt
      541 atactcccgg gcgcagactg gaaatattca aggttgtccg gttgctgcca atagtcaggt
      601 gaagttcagt tgttatcatc agcaaacctg aaaggttgaa aacgcgtatc gttgcctggc
      661 tttccgtttc gctgctgatg ctgcagtcga gtgtggtaac tgccggattg ggaaagatgc
      721 gcattactcc cgtaaccggg atcagatcat tcttcagcga caggacggat gaatattcga
      781 tattattatc gagatccaca tgaaccaccc ggtagctgac gcgacccgcc ggcgtattcc
      841 cgtcggtgaa cgaataagtc tgagcggagc tggtattcag agaggcgatt acgccgactg
      901 aatcgaaatc cgccgagttg accgaacgct ggataataaa ataccgcccg ttagattctt
      961 cccgggcaat ccaggtaaga tgagcattat ggtttatgat ggaaccttca aaattcgtcc
     1021 attgaacggg taaaacgaca accacggtaa cggcctttga ataagaagcc atggtgcaag
     1081 taccgttgga gagcgccgtg ctgactgttt tgactccgag gcttgaccag ctgatgccgg
     1141 atggccgggc cctggcggaa gtcgaagtgc cggcgccagg aaaagaccac ttgtataaag
     1201 cgcctgaggg agcattggta acggcaaaag ccgccggggt cgacaggctg acgctaacgg
     1261 ggccagccac cgaaagggca ttagtggcgg cgggcgccaa ttcgatatcc catacgagta
     1321 ctccataggt gccgctgttg ttgttggaaa ggaactggat atttacctgt ttgtccgcgg
     1381 aacccgacgg aaccgaaaaa gtaaaactgt tatcgtgagc ggaccagttg gtattggtat
     1441 aggcctgtat tttattgaca taattggtgc cggatgccgt gccggtgacc atcacctgca
     1501 aacccggatt cgccggtgtg ccgccgccgg tccttctgct ggcctggtag gtcagcgtat
     1561 agctaaggcc gggaaggata caaacttttt gattgatcgt gcgttccaca tctagctcgg
     1621 tagcgtaatc ggtggacgaa gtgccgccat aaacggattg gggattgatc tccaggctgc
     1681 tgccggtgct ccagccggtg gaagatccgc cggtaaaact cgaattgacc accaggttct
     1741 gtgcgtgcac gcgaaaggtc gtggccagca tcaggctgga aatcacgagc gcctggcaga
     1801 aaaaaaacgt cgggttagcg ttactgcttt tcataaaata aggatcactc gcccaagcag
     1861 tattcctttg tctgtatgcc tgatgatgta aagcaaaaag ctgcaggtcc gcagcttttc
     1921 ccgtccggag taatattata gggtttaggg gtaatcccgg acgttcaact tgttttcagc
     1981 aggatacgga ttaaaatcgg ttttcggttt tcaaaaggat ctcttcggct cttctttgga
     2041 tattggtttt aatggatgta aaactgcaaa ggaaccgggt caaatcataa tcctttcgat
     2101 gaatggagcg aaaatggcga tgagaggaaa gtattgttcg ctggcgaact tcatccgatc
     2161 ccggatcgtt acatgttatt gcgccgcagg atctgtgacc ggaaaatgca gatttacttt
     2221 gtcgtgcatg gtatacaggc gataccttct gccatcgtta accaggatgc cactggattt
     2281 attttctgcc acaacgggat ttcccggata tttcttccag tgaattaaat cgtttgaaac
     2341 ggccacattg gttgaccatt ctttccagtc tttgaatgcc gaggcatgat aatagccata
     2401 gtaaagacca ttgtacctga tcacctggtc catggcgacc gcaaatttat cgtaggcatc
     2461 cgggcccatc gcaataaccg gatcgtcctg cacatttttc catgttttca gatctgtgga
     2521 aacagccagc cagatcccat ggtcatcccg ctcataaaat aaataccagg ctgaatccga
     2581 tttccagacc gcgggtgttc cgtacggccc gggttggatt ggattgccat tggtttgccg
     2641 gatatccagg ttgccctgtt ccctccagtg aatgcgatcc ggagaagtga gtaaatggga
     2701 ggtatcgccc cgtccttctg aaaacaggaa ataaattcca tcggatttga cgacgcacac
     2761 atcttctgcc cagccggcag cgtaaatggg atttgcggga tatctcttcc aggaaatccc
     2821 atccggtgat gtggcgtaac caagatgttt ctcggaatca atatttgtat agccgcaata
     2881 ccacatgtga tattcgctgc cctctttcaa aatccatcct ctttcgcgga tatgccggtc
     2941 ccaggtgctt gtatcggtgc ctgaaaaaat ggggttatgc cggtaagaaa caaaatgaac
     3001 cagttcatcg ggaaattcct tttttgtatg atcggtatga cattgtatga aaaggaaacc
     3061 ggtcagaacg caaagaacct gtttacgcat aaataaaatc cttgtcatcc aaattactga
     3121 atattccccg ggaaccgccg ggaacattac atatcgggaa tttccggctc aaccagcttc
     3181 ttcaattttt ccagggactc ctgccagccc aggtagcaca tttccgccgg gatgacagct
     3241 ggtatatttt cctgcgagat ctttatttcc gtgccgacgg aaactttctg cagcgagacc
     3301 gaggtgatca tttcaccggg cagattggga tcgtcaaatt tatccgtgta ctttaaaaac
     3361 gcattgggtt tgatctcgag atattttcct ccgaaggagt gactgttgcc cgtcgtgaaa
     3421 ttttgaaatg acattttata ggttccgttc acttccgctt tcatttcatg cacggtgcac
     3481 aaaaaaccat agggaggcaa cca
//
LOCUS       DASYCU010000450         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1111480, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000450 DASYCU010000000
VERSION     DASYCU010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111480"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..2123
                     /locus_tag="VGM24_09090"
     CDS             75..2123
                     /locus_tag="VGM24_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS2/Smr-associated SH3 domain-containing
                     protein"
                     /protein_id="HEY4155572.1"
                     /translation="MAAHCRSAYARDKAISLRVHTRREIIERELQQSAEFKTLLQQGQ
                     YFPNEDSLNLSREIKLLGLPGAMLSGEQFLQVRKLADNLRQIFHWFDKERRTAYPALA
                     EVIRDTYYEKQIISSIDEILEENGQVRDRASSELAEIRVSLFRKRNELRRLFDRILSK
                     LSKAGYVAETEEAFLNGRRVLALFAENKRQVKGILHGESDSRKTAFVEPEETIGLNNE
                     IFSLEHAEAREVNRILRELTAKLSGYAPLLANYAGVTGEYDFICAKGRFALDLNGIYP
                     ALQEGSHINLVEAYHPLLFLYHKKSQKPTVPTQIKLDERNRILVISGPNAGGKTVTLK
                     TVGLLQLMIQSGLLIPVHPHSTLGIFKQLMIHIGDTQSLEFELSTYSSHLMNMKYFME
                     QSNGRSLFFIDELGSGSDPNLGGAFAEVILRELLRKHAFGIVTTHYLNLKVMANKTPG
                     IVNGAMAFDEKSLQPLYKMIPGKPGSSYTFSIAERIGLHPDLIQQARALVDEDHFRLD
                     KLLNRTEQDLRNIEQKDRELQQLLKENERLKTEMEKTLHKEKHLQQLELLKEQNKISA
                     ERFVYLKEMERKLKQIIFDWRKAEDKQEAIRQMQALFFHQKERPPSEKLKKKFDSRYM
                     EVEGIIHAGDKVKMKKNHQVGTVREIRGKKAVVQLGLVPITLDMSDLVLIRDRPEENQ
                     "
     gene            2188..2859
                     /gene="rpiA"
                     /locus_tag="VGM24_09095"
     CDS             2188..2859
                     /gene="rpiA"
                     /locus_tag="VGM24_09095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY4155573.1"
                     /translation="MNAKQQAAEKAVSFLESGMTVGLGTGSTAWWAIEKIGEKIKKDH
                     WKIRAIATSLRSEEQARSLGIPIFGFAEIGMIDVTIDGADEVDGQLQLIKGGGGALLR
                     EKIVATNSRQMIVVADESKKVTTLGKFPLPVEVVLFGWERTLEKLKALGCETKRRMDR
                     NDPFLTDNGNYIVDCDFGRIPDPASLHERINGITGVVDNGLFINIASKLVLGFESGET
                     RIITR"
     gene            2872..3999
                     /locus_tag="VGM24_09100"
     CDS             2872..3999
                     /locus_tag="VGM24_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY4155574.1"
                     /translation="MGESSFIRSIRSGVRFLLKRPVTWIANRVSSAPDRTAVLEALTE
                     LYQEAGSPKNKKVNHLSFSENKLKIIILSDQHRGTRDGSDDFAVCESSYLDALDYYNR
                     EQFYFINLGDCEELWENTIFGIMKHNPAVFDREKQFIDRLAYCKVFGNHDLFWDNDPL
                     AGAYLKKIYGTAIPVFTGVVLRVMLSEGGSFDIFCSHGHQGDRQSDGNAFSKWFVSYI
                     WGPLQSLLEININSPSANDNLKTLHNLYMYDWSAAQKNVLLVTGHTHQPVFNSMTHLE
                     RLYFKLEAAEKAVDKDAIRKIEAEIPRRKREYDFVNHSFRNMKPSYFNSGCCCFDDGT
                     ITGIEISEGFIRLIKWSLVNGKPRRIVAEEERLADVATGIR"
     gene            complement(4024..4920)
                     /locus_tag="VGM24_09105"
     CDS             complement(4024..4920)
                     /locus_tag="VGM24_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY4155575.1"
                     /translation="MEFGRIDPASLAGTDFRLPKEPDWNKSILKGRKSNPSVYLGCAK
                     WGRKEWVGKLYPAKTREKDFLSHYVQHYNSIELNATHYKIYGPAGIERWNGLAANKDF
                     KFCPKMYQGITHRGSLKGKDFLITEFLRGVLAFEDHLGPVFIQFSESFGPKRKEEIFL
                     FLRSLPVDLSFFVEIRHPEWFLNESVFHELLSTLLSLNMGIVITDTAGRRDCCHMHLT
                     IPKTFIRFVGNSLHPSDYTRCDEWVERIKYWIGAGMNELYFFMHMHDEAYSPELTYYL
                     VKKLNDPVKLNLPVPALLTEKK"
BASE COUNT         1414 a         1249 c         1390 g         1273 t
ORIGIN      
        1 ctttgttttg aattatcaga tacttcccgg aatcggcact tacccaactg gaattcgata
       61 agatccggga gcttttggcc gcccattgca gatcggccta tgcccgggac aaggccatca
      121 gcctgcgggt ccatacccgc cgggagatca tagaacggga gttgcagcaa tcggccgaat
      181 tcaaaaccct tcttcagcag ggccagtatt ttccaaatga agacagcctg aatctttccc
      241 gggagatcaa attgcttggc cttccgggcg ccatgctcag cggcgaacag ttcctgcagg
      301 tccgtaaact ggcagataat ctccggcaga tcttccactg gttcgataag gaaagaagga
      361 ccgcataccc ggcattggca gaagtgatcc gggatactta ttatgaaaaa cagatcatct
      421 cctctataga tgagatcctg gaggaaaacg ggcaggtccg ggatcgtgct tcttctgaac
      481 tcgcggagat ccgggtatcg cttttcagaa aaagaaatga gctccggcgg ctgttcgacc
      541 ggatcctatc gaaactcagc aaggccgggt atgtggctga gacggaagag gcctttttaa
      601 atggccgccg cgtgctggcc ctgtttgcgg aaaacaagcg acaggtaaaa ggcatcctgc
      661 atggggaaag cgatagccgg aaaacggctt ttgtggaacc tgaagaaacg attggtttga
      721 acaatgagat tttttccctg gaacacgccg aggcccggga agtgaaccgg atcctgcgcg
      781 aacttacggc aaaactatcc gggtatgccc ccttgctggc caactacgcc ggggtcacgg
      841 gggaatatga ttttatctgc gccaagggca ggttcgccct ggatttgaat ggcatctatc
      901 cggcattgca ggagggttcc catattaacc tggtggaagc ctatcatccg ctcttatttc
      961 tatatcataa gaaatcccaa aagccaaccg ttcccacgca gatcaaactc gatgaacgca
     1021 accggatcct tgtgatttcg ggaccgaatg ccggcggaaa gacggttacc ctgaaaacgg
     1081 tgggcctgct tcagctgatg attcaatccg gcctgctgat ccccgtgcat ccgcactcca
     1141 ccctgggcat tttcaaacag ctgatgatcc atatcggcga cacccagagc ctcgagtttg
     1201 agctgagcac ctatagttcc cacctgatga acatgaaata tttcatggaa cagtccaatg
     1261 gacgcagcct gtttttcatt gatgagctcg gcagcggcag cgatccgaat ctgggcggtg
     1321 cttttgccga ggtcatcctg cgggaactgc tccggaaaca tgcttttggt attgttacca
     1381 cccattatct caacctcaaa gtgatggcca ataaaacgcc gggcatcgtt aacggcgcca
     1441 tggcatttga tgaaaaaagc ctgcaacccc tgtataagat gattcccggg aaaccgggaa
     1501 gttcctacac tttttccatt gccgagcgca tcggcctgca tccggatctt attcaacagg
     1561 cccgggcgct cgtggatgaa gaccatttcc ggttggataa actccttaac cggacagaac
     1621 aggacctccg gaatattgaa caaaaagacc gggagctgca gcagctgctg aaggaaaatg
     1681 agcgtctgaa aacggagatg gaaaaaacgc tgcataagga aaaacacctt cagcaactgg
     1741 agcttttgaa agagcagaat aagatttcag ccgaacgttt tgtttatctg aaagaaatgg
     1801 aacgtaagct gaaacagatt atattcgact ggcggaaggc ggaagataaa caggaagcga
     1861 tccggcagat gcaggcactt ttttttcacc agaaagagag gcctccgagc gagaagctga
     1921 aaaagaaatt tgactccagg tatatggaag tggaggggat cattcatgcc ggagataaag
     1981 tgaagatgaa aaaaaatcac caggtgggaa cggtccgcga gatccgggga aagaaagcgg
     2041 tggtgcagct gggcctcgta ccgattaccc tggatatgtc ggatcttgtc ctgatccgcg
     2101 accggccgga ggaaaaccaa tgatctaccg gaaatggacg accgaaaata aaaaagagtt
     2161 ccctccggaa ccgcataacg gtaatttatg aatgcaaaac aacaggccgc ggaaaaagca
     2221 gtctctttcc tggaaagcgg aatgaccgtg gggctcggta cgggttctac cgcctggtgg
     2281 gctattgaaa aaataggcga aaagattaaa aaggatcatt ggaagatccg ggcgatcgcc
     2341 acctcacttc gttcggagga acaggcccgt tccctgggca ttcccatatt cggctttgcg
     2401 gaaatcggaa tgatcgatgt gaccattgac ggcgcggatg aggtggatgg gcagctccag
     2461 ctgatcaaag ggggcggggg cgccctgctc cgcgaaaaaa tcgtggcgac caacagccgg
     2521 cagatgatcg tggtggcgga tgaatccaag aaagtgacca ccctggggaa atttccgctt
     2581 ccggtggagg tcgtgctttt tggatgggag aggacgcttg aaaaactgaa ggcattgggc
     2641 tgtgaaacga agcggcggat ggaccggaac gacccttttc tcaccgacaa cggaaattat
     2701 atcgtggatt gcgacttcgg aaggattccc gatccggctt ccctgcacga gcggattaac
     2761 gggattaccg gtgtggtaga caacggactt tttattaata tagcaagcaa actggtgctg
     2821 ggttttgaat ccggggaaac ccggatcatt accagataga ttaattccgg tatgggcgaa
     2881 tcctctttca tccgatccat tcgcagcggc gtccggttct tgctgaaacg gccggtcacc
     2941 tggattgcca accgcgtatc ttccgccccg gaccggactg cggtcctgga ggcgctgacg
     3001 gaattgtatc aggaggcggg cagccccaaa aacaaaaagg tcaaccatct ttccttttcc
     3061 gaaaacaagc ttaagatcat cattctgagc gatcagcacc ggggtacccg ggatggttcc
     3121 gatgattttg cggtttgcga atccagttac ctcgatgccc tggattatta taaccgggaa
     3181 cagttttatt tcataaatct gggtgactgt gaagagctct gggagaatac catttttggt
     3241 atcatgaaac acaaccccgc cgtattcgac cgcgagaaac aatttatcga ccggctggcg
     3301 tattgtaaag tatttggcaa tcacgatctt ttctgggata atgatccgct ggctggcgcc
     3361 tatctgaaaa aaatttacgg aacggctatt ccggttttta ccggagtcgt gctgcgggtt
     3421 atgctatctg aaggcggttc cttcgatatt ttctgctccc atggacacca gggagaccgg
     3481 caaagcgatg gaaatgcttt cagtaaatgg tttgtgagct atatctgggg cccgcttcaa
     3541 agtttactgg aaataaatat caacagccct tctgccaatg acaatttaaa aacacttcat
     3601 aatctgtata tgtatgactg gagcgcggcc cagaaaaatg tattgctggt aacgggtcat
     3661 acgcaccagc ctgtttttaa ttccatgacc cacctggaaa gattgtattt taagctggaa
     3721 gcggcagaaa aggccgttga caaagacgct atccggaaga tcgaggccga aatacccagg
     3781 cgtaagcggg aatatgattt tgtgaaccat tctttcagaa atatgaaacc atcctatttc
     3841 aattccggtt gctgttgctt tgatgacggc acgatcaccg gaatagagat cagtgaaggg
     3901 tttatccgcc tcattaaatg gagcctggtc aacgggaagc cccggcggat cgtggcggaa
     3961 gaagaaaggc tggcagatgt tgctacagga atccggtagc ttcggcgacg gccggaaagc
     4021 gggtcatttt ttttccgtaa gcagagccgg tacgggaaga tttaatttga ccgggtcatt
     4081 cagttttttc accagataat acgtgagttc gggagaatag gcttcatcgt gcatgtgcat
     4141 gaaaaaatac aattcattca tccccgctcc gatccagtat tttattcttt ccacccattc
     4201 atcgcagcgg gtgtaatcgc tggggtgcag gctgtttccg acaaaacgga taaaggtctt
     4261 gggaatcgtc aggtgcatat gacaacaatc ccttctgccg gcggtatcgg tgatcacaat
     4321 gcccatattc agagacagca gggtggagag caattcgtga aatacggatt cattaaggaa
     4381 ccattccggg tgcctgattt ccacgaagaa agaaagatcc accggcaggg accggagaaa
     4441 gaggaaaatc tcttcttttc tttttggccc gaaggattca ctgaactgga tgaagacagg
     4501 ccccaggtga tcttcgaatg ccagcacgcc tcggaggaat tcagtgatta gaaaatcctt
     4561 tcccttcaaa ctgccccggt gtgtgattcc ctgatacatt ttggggcaaa atttaaagtc
     4621 cttatttgcc gccagcccgt tccagcgttc aataccggca ggcccgtaga tcttgtaatg
     4681 cgtggcattc agctcaatgc tgttgtaatg ctgcacataa tgggagagaa aatccttttc
     4741 cctggtcttc gcggggtaaa gctttcccac ccattccttg cgcccccatt ttgcgcatcc
     4801 caggtaaacg gagggatttg attttcttcc tttaagaatg gatttattcc aatccggttc
     4861 ttttggtaat ctaaaatccg tcccggcaag gcttgccggg tcaattcttc caaactccat
     4921 agactgacgg gttaatatga aacgggaaaa tgcgcaaaat aggccaggag gaatgagaaa
     4981 ttttaacctt tctgcatttc cggaggatac tgaggcattt attctcgtgt ttgctgctta
     5041 ttttcctcaa aaatccttcc agataccaaa tatcaggcta attacgggta ataaaatctg
     5101 gtacgatcct ttcagtacaa aaggtaattg tataactcga acaggattgc agaggatgga
     5161 atatgagctg tgaaatcctg ttcttgatct gcctgagcgg atataataac taaaggctct
     5221 gtctgaccga cggagccttt agtttttgtg gactataaaa atcacagggt ctacatgctg
     5281 gcgtgcagat aatcttttaa ctgcgagatg gtttcctcct ggtgct
//
LOCUS       DASYCU010000451         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1838928, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000451 DASYCU010000000
VERSION     DASYCU010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838928"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VGM24_09110"
     CDS             <1..296
                     /locus_tag="VGM24_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4155576.1"
                     /translation="SNTVQISQNPGDQISVYPNPATRLLNISIQGGQNQRYQVILYNI
                     AGQILYTGSPVNVQNSTLTYQREPAVAAGLYFLKVKNLSSGESSTFKIVFGER"
     gene            complement(368..544)
                     /locus_tag="VGM24_09115"
     CDS             complement(368..544)
                     /locus_tag="VGM24_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155577.1"
                     /translation="MKSRKLLIGAGIAITAVLIIQLVRTRKKREKKRIHIANEGYEMA
                     EDILYPKRKSLKLS"
     gene            complement(575..1009)
                     /locus_tag="VGM24_09120"
     CDS             complement(575..1009)
                     /locus_tag="VGM24_09120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY4155578.1"
                     /translation="MGISDKLFQLKNEKAAANLKAGHDFLEINRQRPGVVALPSGLQY
                     EVIAEGNGVKPGATANVTCHYHGTLIDGTVFDSSVKRGQPAAFPLNMVIKGWTEGLQL
                     MPVGSKWRFFIPPELGYGNRQVSAEIGPNSTLIFEVELIEVN"
     gene            1109..>1578
                     /locus_tag="VGM24_09125"
     CDS             1109..>1578
                     /locus_tag="VGM24_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4155579.1"
                     /translation="MRFQFNSLPFYHKLASVLVSLIAIGYLVILSKDLLSPVIFSCLF
                     SILLLPLASFLEKKFRLPRGAASMLAVLLLLAAISLIVYVVGSQISTLSDDWPQFKQQ
                     LTGSLADLQHWISRKYHINAHKQLNYVQSATSQVLASGTTVVGATIMSISSILL"
BASE COUNT          374 a          424 c          323 g          457 t
ORIGIN      
        1 acagcaatac ggtacagatc agccaaaatc ctggggatca gatctcggta tatcccaatc
       61 cggcgacccg gcttttaaac atcagtattc agggaggcca gaatcagcgt taccaggtga
      121 ttttatacaa tattgccgga cagatcctct ataccggctc gcctgtaaat gttcaaaaca
      181 gcaccctcac ttatcagcgg gagcctgccg tcgccgcggg attatatttt ttgaaagtga
      241 aaaatctttc aagcggggaa agcagtacgt ttaaaattgt gtttggggaa cgatgaaaag
      301 attgatggaa tatcgcgaat agccatcagc cgtgaatcca caaatgatct ccatccggga
      361 tgcagcatca actcaactta agggatttcc gcttcggata taaaatatcc tccgccattt
      421 catatccctc attggcaata tgtatgcgtt tcttttctct tttttttctt gttctgacga
      481 gttgaataat caggaccgcg gtgatcgcaa tccctgcacc gatcagcagt tttctacttt
      541 tcattccgga aagttatcgt tctgaatatt atttttagtt gacctcaata agctccactt
      601 caaaaatcag ggtactgttc ggacctatct ctgcactgac ctgccggttc ccgtacccca
      661 gctcgggtgg aatgaaaaag cgccatttgc tgcccaccgg catcaattgc aaaccttcgg
      721 tccaaccttt aatgaccatg ttcagcggaa aagcagcggg ctgaccccgt ttaaccgaac
      781 tgtcaaatac agtgccatcg atcagtgttc catgataatg acaggtcaca ttagcggtgg
      841 cccccggctt taccccgttt ccctctgcga ttacttcata ctgcagaccg ctgggcagtg
      901 caaccactcc cgggcgctgt ctgttgattt ccaaaaaatc atgcccggct tttaaattgg
      961 cggccgcttt ctcattcttc agctgaaata atttatcact tatgcccata acggattatt
     1021 tatgcgaagt taatttaaac tacttttatc ccgtctttat caccccattt ttactttatt
     1081 ataaccattg ggagaataat ccttttttat gcgctttcaa tttaacagcc tgccattcta
     1141 tcataaactg gcttccgtgc tggtaagcct gatcgccatc ggctacctgg ttatcctttc
     1201 gaaagattta ctgagccccg tcattttttc ctgcctgttt tccatcttgc tgcttccgct
     1261 cgccagcttc ctggaaaaga aattccgttt gccaaggggt gccgcttcca tgctggccgt
     1321 tttgctgttg ctggcagcca tttcgctcat cgtgtatgtg gtcggttccc agatcagcac
     1381 actttccgac gactggcccc agttcaaaca acagctgacg ggttcgctcg ccgatctgca
     1441 gcactggatt tcccgcaaat accatatcaa tgcgcataag cagctaaact atgttcagtc
     1501 ggccacttcc caggtgctgg catccggaac gaccgtggtg ggcgccacga tcatgtccat
     1561 ttcctccatc ctcctgtt
//
LOCUS       DASYCU010000452         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1983558, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000452 DASYCU010000000
VERSION     DASYCU010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1983558"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VGM24_09130"
     CDS             complement(<1..397)
                     /locus_tag="VGM24_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4155580.1"
                     /translation="MRQLKIATQITNRDSQAVEKYLQEISKISMITPEEETILAQRIK
                     MGDQKALDKLAQSNLRFVVSVAKQYQHQGLSLSDLINEGNLGLIKAAQRFDETKGFKF
                     ISYAVWWIRQSILQALAEQGRLVRLPQNKI"
     gene            700..1080
                     /locus_tag="VGM24_09135"
     CDS             700..1080
                     /locus_tag="VGM24_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY4155581.1"
                     /translation="MNTGKTLTKSEEQVMHVLWKLGKGFLKEIIESTPEPKPHPNTIA
                     TLLKILVEKGFVEYTVHGRNNLYKPLIGRTEYGKKSANQLLKGYFEGSPARLVSHFIS
                     DHKLSEAELEQLLQQIQSAKNNKS"
     gene            1077..3113
                     /locus_tag="VGM24_09140"
     CDS             1077..3113
                     /locus_tag="VGM24_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY4155582.1"
                     /translation="MSLLFYLLKTVLISGLLLGYYWLFLRNKSFHRYNRYFLMGIPVI
                     SLILPGLRFNLPQFWGQNPGNNPIHLLGVANGGLEEAVTVYARQGHWKGLPWESLCLA
                     LAMLFSLVFLFRFYRSLRLIYILRRRHPFFESQGARIYLVRQEGTPFSFFKNIFWNES
                     KELNSPQGRQILRHELYHVQHKHSLDIVLLEIARIVFWFNPFIHLIRLEIQAIHEFLA
                     DAYAASETDRLEYAELLLVNSIRPKSISITHPFFQNPIKRRIVMITTNKNIRSGLLRR
                     MMILPVIALLLGLFAFKLQNHSLLFPAKTKNVRVVIDAGHGGIYTGAQANGVFEKDIN
                     LQIAKKIQELAKDYQVEVVMSRDGDRLPEQFKNLREDLLYRTALPARTNADLLVSIHL
                     NSADKSGDHPAGGFDIYVPGNNSGVYPGSVKLGSGISEFIKKDYSISPELKQARILEL
                     DNATVPSVLIECGYIENKTDLDFITNDKNQEAIARDILEGIRQYSQDKSSMQDDPAAS
                     RTDTLVADTMTIEALRSSDPNTIYSINVDKKNNRIYVKFKNGKENVVLVTKEVKMVLD
                     TGGAYRKVEIEAKFPGGSSGWMQYLQKSLKYPDEAASKAIQGSVMVEFIVNTDGTLEN
                     IHVLSGPEALKAESIRVIRESGKWTPAKDQGKIVKSYHKQPIIYRLEPVDRRLP"
BASE COUNT          866 a          784 c          771 g          769 t
ORIGIN      
        1 cgatcttatt ctgcggcagt cgaaccaggc ggccctgttc ggccaaagcc tgaagaatgg
       61 actgacggat ccaccaaacg gcataggaaa taaatttgaa ccccttggtt tcatcaaagc
      121 gctgggcggc cttgatcagg cccagattgc cttcattaat gaggtcgctc agggaaagcc
      181 cctgatgctg gtattgcttc gccacggaaa caacaaagcg gaggttggac tgggccagct
      241 tgtccaaagc cttctgatcc cccatcttaa ttcgctgtgc cagaatggtt tcttcttcgg
      301 gcgtgatcat ggagatcttt gaaatttcct gtaaatactt ctctaccgcc tgcgagtcgc
      361 gattggtaat ttgcgttgca attttaagtt gcctcataat gaataataaa ttgaagtgac
      421 aaagttcttt gtcgggtaca aatcaaccat ataacaatgc tgaccgcgga aaagttgaaa
      481 aggggtatga ttaaaaatga actccgcgct gttctattac aaaagtaata cccattcccg
      541 gaacttcata gcctttctcc cttttaaaaa ataaaaattt ggataaacta attctttagt
      601 tatatatttg ttcaataaac taaaaattta gttatatgaa acccgctcag gacggggctt
      661 tcagagcggc taaatagata cacttaaaaa aagagaatta tgaatacagg caaaaccctt
      721 acgaaatccg aggaacaggt gatgcatgtg ctctggaaac tggggaaagg tttcttaaaa
      781 gagatcatcg aatccactcc cgagccaaaa ccccatccca ataccatcgc caccctgttg
      841 aagatcctgg tggaaaaagg ctttgtggaa tacacggtgc atggccgcaa taacctgtac
      901 aagcccctga tcgggcgcac cgaatacggc aagaaatcgg ccaaccagct gctgaagggg
      961 tatttcgaag ggtcgccggc ccggctggtc tcccatttta tcagcgatca taaattgagt
     1021 gaggcggaac tggaacagct tcttcaacaa attcagtcag ctaaaaacaa caaatcatga
     1081 gtttgctttt ttatctgctc aaaaccgtgc tgatctcggg tctgctcctg ggatattact
     1141 ggcttttcct tcggaacaag tcctttcacc ggtacaaccg gtacttcctg atgggaattc
     1201 cggtgatcag cctgatcctg ccggggctcc ggttcaacct gccccaattc tggggtcaaa
     1261 acccgggtaa caacccgatc cacctcctgg gcgtcgccaa tggcggtctg gaggaagcag
     1321 tgaccgttta tgcgcggcag ggacactgga agggcctgcc ctgggaatcc ctttgccttg
     1381 cgctggccat gctgttttcc ctggtctttt tattccggtt ttatcgctct ctccggttga
     1441 tttacatcct ccgccggcgc catccgttct tcgaatcgca gggtgcccgc atttacctgg
     1501 tcaggcagga aggaactcct ttttcctttt tcaaaaatat attctggaac gaatcaaagg
     1561 aattgaacag tccccagggg cggcagatcc tgcggcatga attgtatcat gtacaacata
     1621 aacattccct ggatatcgtg ctgctggaga tcgcccggat cgtattctgg ttcaatccct
     1681 ttattcatct gatccgtttg gaaatccagg ccattcatga atttctggcc gatgcctatg
     1741 cggcttccga aacggaccgg ctggagtatg ccgaattatt gctggtgaac agcattcgtc
     1801 ccaaatccat ttccattact cacccattct ttcaaaaccc gattaaaaga agaatcgtca
     1861 tgattacgac aaacaaaaac atcagatccg gcctgctgcg cagaatgatg atcctgccgg
     1921 tgatcgccct gctcctgggc cttttcgctt tcaaacttca aaatcattcc ctgctcttcc
     1981 ccgccaaaac aaaaaacgtc cgcgtggtca ttgacgcggg acatggtggt atttatacgg
     2041 gcgcgcaggc aaatggcgtt ttcgaaaaag atatcaacct gcagatcgcc aaaaaaattc
     2101 aggagctggc caaagattac caggtggaag tggtcatgag ccgggatggc gaccggcttc
     2161 cggagcaatt taagaacctg cgggaagatt tactttaccg caccgctctt ccggccagga
     2221 caaatgccga tctgctcgtg tccattcacc tcaattcggc agataaaagc ggggaccatc
     2281 ccgccggggg ttttgatatt tatgtacccg gaaacaacag cggggtttat cccggttccg
     2341 tgaaactggg ttccggcata tccgagttca ttaaaaagga ttattccatt tccccggaac
     2401 ttaaacaggc gcggatcctt gaactggata atgcaacggt tccttccgtg ctgatcgaat
     2461 gcggatacat agaaaacaaa accgatctcg attttataac caatgataaa aaccaggaag
     2521 cgattgcccg ggacatactg gaaggcattc gccaatacag ccaggataaa agcagtatgc
     2581 aggatgatcc ggctgcttcc cgcacagaca cgctggtcgc cgatacgatg accatagagg
     2641 cactgagaag cagcgatccg aatacgatct attccattaa tgtcgataag aaaaacaacc
     2701 ggatatacgt gaagtttaag aacggaaagg agaacgttgt actggtaacc aaagaagtga
     2761 agatggtgct ggatacgggc ggagcatata gaaaagtgga aattgaagcg aaatttcccg
     2821 ggggttccag cggctggatg cagtatcttc agaagagcct gaaatatccg gatgaagccg
     2881 ccagtaaagc gatccaggga tccgtgatgg tggaattcat tgtgaacacc gacggaacgc
     2941 tggagaatat tcatgtgctc agcggaccgg aagccctgaa ggcagagtcc atccgggtga
     3001 tccgggaaag tggaaaatgg acgccggcaa aggatcaggg gaaaatagtc aagtcctacc
     3061 acaagcagcc cattatctac cggctggagc ctgtagaccg gaggcttccg tagaatggaa
     3121 ttggtccgcg cgttctccga ccggttcagt acccgatctg attcgttccc gtatcgatca
     3181 gcaactcttt
//
LOCUS       DASYCU010000453         7511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3288061, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000453 DASYCU010000000
VERSION     DASYCU010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288061"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGM24_09145"
     CDS             <1..675
                     /locus_tag="VGM24_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155583.1"
                     /translation="SVIAFLANVFIAPTWVHEQFNLYLLRMMEANKSYFRDVAATFTG
                     EQVDIIQYKYSRKMAYVELANTTDALNRMLAEPRRKQKNPAEFHQFVVLNYMMASHIA
                     TLASYARSGTPFPVDRDYLPVLNAVLSNLSKAGSLLQGNTDKTHTAQKAEQSEPAEGS
                     TAKEGLRKMNDRVSAMLVKRRAELEQGIQDSPTRRQLTLVKSINDQFNFITKISEDLL
                     KVVAKW"
     gene            849..1880
                     /locus_tag="VGM24_09150"
     CDS             849..1880
                     /locus_tag="VGM24_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEY4155584.1"
                     /translation="MKRRNFIRQTALASGGLLIMHDLLAARKEKVYGYNGMTYSLDTA
                     WSKANAAELPVNDCHEMVQDSRGRIILLTNETKNNILIYNTDGKVLDNWGHLFPGGHG
                     LTLHNENGTEFFYITDIVKHQVYKTTLDGKLLLTIDAPLDAGIYSKPEEFVPTETVID
                     QNGDIFIADGYGAQYILQYDRQGKLLHYFGGRGDGDEHLDNAHGICFDTRNDLHSLLV
                     TDRTRNCFKRFSKDGRLLEVIRLPGACVCRPVIKDDHLYAAVLRSPDMNTSGTGFVTI
                     LDKQNKVVSNIGGSEPKYLGGQLQPMGQTDKIFIHPHDVCVDRDDNIYVAQWASGKVY
                     PYKLTRANG"
     gene            1883..2875
                     /locus_tag="VGM24_09155"
     CDS             1883..2875
                     /locus_tag="VGM24_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY4155585.1"
                     /translation="MDFVKEVYDWAVGFFGVADISKIIHSGNYSSLRTYKGIVAVIGP
                     LFPVILVVEIFVALLYRKLKLIDYKIPFFSYVLNAVVGRFISIAAIAYCIGLFEKHAI
                     FKTSFTWYWFLYGYVVWEFGHFLYHYFGHKVRLFWCLHSTHHAPQNMNLSISFAHFFL
                     EGPYADVIRTTTCILLGVSPPLLFAIMFIDGSWGGFIHLGETMSKTGKFGPLHKYILT
                     PMHHRVHHGRNPLYMDTNFCNLLNIWDRVFRTYQPINPDIPVEYGITRKMVPNSFMDA
                     YFGEFVALAKDIARAPGIKNKFLYIIMPPGWSHTGEHKTASKVRGDFLKSLKRS"
     gene            complement(2883..4700)
                     /locus_tag="VGM24_09160"
     CDS             complement(2883..4700)
                     /locus_tag="VGM24_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY4155586.1"
                     /translation="MIKFAGDSGDGMQLTGSQFTNNTALLGIDLATFPDFPAEIRAPQ
                     GTLPGVSGYQLHFSSDHIFTPGDECDVLVAMNAAALKTNLKTLKKGGRIIANVEGFDA
                     KNLRLANYPEGINPLEDHSLGNYDLIRIDVTKLTREALKDFALGNKEKDRAKNMFVLG
                     FLYWMYNRSLESTIQFLTEKFGKNPDIMESNIRALRAGFNFGDTTETFTHTYTVAKAR
                     LQPGTYRSIMGNQALAYGLIAASQKSGLPIFLGTYPITPASDILHELSKQKAFGIRTF
                     QAEDEIAGITAAIGASYGGSLGVTSTSGPGMALKSEAMGLAMMLEIPLLICDIQRGGP
                     STGLPTKTEQSDLLQAYYGRNGESPMPVVSASTPSDCFDAVYEAARIAIQHMTPVIFL
                     SDGYIANGSEPWKFPDAEHLHPIEVKFKTELGDGEDKLQPYLRDERGVRPWAIPGTPG
                     LEHRIGGLEKQNITGNVSYDPENHHLMVKLRQEKVDKIAEHIPLQKLDSGPEKGKLLV
                     VGWGSTYGSIKTAAAELQREGYAVSHAHLRYLRPFPRNLGHLIENFEQVLVPELNNGQ
                     LIKIIRDQFLVNALPYNKIMGIPFTRTELIVKMREMLEG"
     gene            complement(4772..>7511)
                     /locus_tag="VGM24_09165"
     CDS             complement(4772..>7511)
                     /locus_tag="VGM24_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY4155587.1"
                     /translation="ISFNNVQVEKDILRYDVGGICLFQGGPKTQAAFINHFQQIAKTP
                     ILFSIDAENGLGMRMDSVGSLPRQMMLGAVQDPQLIYQYGQLVGEQCKRMGIQINFAP
                     VVDVNNNPDNPVINDRSFGEDQYRVAELGIQYMKGLQATGIMAVAKHFPGHGDVSVDS
                     HYDLPVINKTMNELDTLELYPFKKLIDAGVQGIMTGHLFVPAIDKKPNTPASISYNAV
                     TKLLRRKLKFSGLSFTDALEMKGVTKYYPDGDASVQSLIAGNDVLCLPGDVELSISKI
                     LQAVKEKKLKWKDLNDRVKKVLAAKYQLGLTRLQPVDTTHLIEDLNKDVRDMYVKVAH
                     LSLTLLRRNDRSLPESMAGKRVAYIGFGLANDNEFARRVREDYHANVYYFDYSLDSLK
                     AAAAYELLRNRYDMLIIGMHNLNRYPAHDFGISPAALSLLKKLEQQSGVISFVFGNPY
                     VIKYVCNAQNLLECYDDNEITQSVAADWLNGKFLPRGRLPVSVCAEMKAGYGITFTRG
                     LLPEAPPASVGFEPSRLLEIDSICKRAIELQATPGCVVLVARNGRIAYEKAFGYMQYD
                     KKDPVYPETIYDLASVTKICATTISVMKLYDEGKLDLNKTLGDYLPWVRGSDKDTLKI
                     WDIMLHQAGLKAFIPFYAETITKDKEGLPLPSVYSSVGDSLHCVRVAEHLYMRSDWLD
                     TMYKRILTSPLGRVGNYIYSDNDFIFMGKIVEAISGKTLDQYAYDNFYVPMGLTSTGF
                     KPRNRFPLNRIAPTQLETAFRRQLIHGDVHDPGAAMFGGVSGHAGLFSDAYDLGVILQ
                     MLINGGIINGHRYLKKETIDYFSEYHSGISRRGLGFDKPQKDNATNPDPYPCVSASPQ
                     SYGHSGFTGIWIWDDPVKQLVFVFLSNRVNPVESNKISSLNVRGNILEAVYKAMKN"
BASE COUNT         1802 a         1910 c         1876 g         1923 t
ORIGIN      
        1 tcggttattg cttttctcgc caatgtattc atcgctccca cctgggtgca tgaacagttc
       61 aatctttatc tcctgaggat gatggaggca aataaaagtt atttccggga cgtggcagct
      121 acgtttacgg gagagcaggt ggacatcatc cagtataaat attcccgtaa aatggcttat
      181 gtggaactgg ccaatacgac ggatgccctg aaccggatgc tggccgagcc ccggcgaaaa
      241 cagaaaaacc cggctgaatt tcatcagttc gtcgtgctca attatatgat ggcttcccat
      301 atcgcgaccc tcgcttccta tgcccggagc ggcactccgt ttccggttga ccgggattat
      361 ctcccggtat tgaatgccgt actatccaat ctctccaaag cgggttccct tttacaggga
      421 aacaccgata aaacccatac cgcgcaaaag gcggagcagt cagaacctgc agaaggaagc
      481 acagccaaag aaggattgag gaaaatgaac gatcgcgtat ccgccatgct ggtgaaacgc
      541 agggcagaac tcgagcaggg tattcaggac agtcccacgc gcagacagct aaccctcgtc
      601 aaatcaatca atgaccagtt caattttatc acgaagatct cagaggatct tttgaaagtg
      661 gtggcgaaat ggtaacccct tcagccaata actccacttc cccgcgtatt tttattttcg
      721 gtaattttat ggggagtttt cggacggcca tccaccccgc tttgctgtca tttgaaaaac
      781 aggcgttcag gcgccctgga aaaggccagc gggtaaacca tcagcataaa acccaaaaga
      841 ggtaaatcat gaaaagaaga aattttatcc ggcaaacggc tttggcaagc ggtggtttgc
      901 tgatcatgca cgacctgttg gccgccagga aagaaaaggt atacggctat aatggcatga
      961 cctattcgct ggatacggcc tggtccaaag cgaatgcggc tgaacttccg gtaaatgatt
     1021 gccatgaaat ggtgcaggac agccggggcc ggatcatcct gctgaccaac gaaacgaaga
     1081 ataatattct catttataat accgatggaa aggttttgga taactggggc catttatttc
     1141 cgggtgggca cgggctgacc ctccacaatg aaaacggaac cgagtttttc tatatcaccg
     1201 atattgtgaa acaccaggtc tacaaaacca cccttgatgg aaaattgttg ctgaccatcg
     1261 atgctccgct cgatgcaggg atctattcca aaccagaaga atttgttccg acagaaaccg
     1321 tgatcgatca gaacggagat atttttattg cagatggata cggggcccag tatattttgc
     1381 agtacgaccg tcagggaaaa ctccttcatt atttcggcgg ccgcggcgat ggcgatgaac
     1441 acctggataa tgcccatggc atttgctttg atacccggaa cgatctccat tctctgctcg
     1501 ttaccgacag aacccggaat tgcttcaaaa gattttcgaa ggatggccgg ttactggagg
     1561 tgatccggtt gcccggagcc tgtgtctgca gacccgtcat taaggacgat cacttgtatg
     1621 ccgccgtgct ccgctcgccc gatatgaata ccagcggaac cggttttgtt acgattctcg
     1681 acaaacaaaa caaggtggtc agtaatatcg ggggcagcga acccaaatac ctgggtggcc
     1741 agttgcagcc gatgggtcag accgacaaaa tatttattca tccgcatgat gtttgcgttg
     1801 accgggatga taatatttat gtggcccaat gggcatcggg gaaagtttat ccgtataaat
     1861 tgacgagagc gaatggatag ttatggattt tgtgaaagaa gtatatgatt gggcagttgg
     1921 cttttttggc gtagccgata ttagcaagat cattcattca ggtaactaca gttctctccg
     1981 cacctataag ggcatcgtgg ccgttatcgg tcccttattc ccggtcattc tggtggtgga
     2041 gatctttgtt gctctcctat acaggaaatt aaagcttata gattataaga tcccgttttt
     2101 ttcctatgtc ctgaatgcgg tggtgggacg gtttatttcc attgcagcga ttgcctactg
     2161 catcggcctt tttgaaaagc atgccatttt caagaccagt tttacctggt actggtttct
     2221 ctacggttat gtggtctggg aattcggtca ttttctttac cattacttcg gtcataaggt
     2281 ccggctgttc tggtgcctgc actccacgca ccacgccccg caaaacatga acctttccat
     2341 ttctttcgcc catttttttc tcgagggccc ttatgcggat gttatccgga cgaccacctg
     2401 tattctgctc ggggttagtc ctcctttgct atttgccatc atgtttatag acggctcctg
     2461 gggtggtttt atccatttgg gggaaaccat gagtaaaacc gggaaattcg gacccctgca
     2521 taaatatatc ctgacgccca tgcatcaccg ggtccatcac ggccggaatc ccctgtacat
     2581 ggataccaat ttttgtaacc tgctgaatat atgggaccgg gtcttccgta cctatcaacc
     2641 catcaacccg gatatcccgg tggaatatgg aattacgcgg aaaatggtgc cgaacagttt
     2701 tatggatgcc tatttcggtg aattcgtcgc gctggccaaa gacatcgcca gagcgcccgg
     2761 catcaaaaac aaattcctct acatcatcat gccacccgga tggagccaca ccggagagca
     2821 taaaacggca agtaaagtga gaggcgattt tctgaagagt ttgaaacgat cgtgaaattc
     2881 gactatcctt ccagcatttc cctcattttc actatcagtt ccgtccgggt aaacgggata
     2941 cccatgatct tgttataggg aagggcgttg acgagaaact gatcccggat aattttgatg
     3001 agctggccgt tgttgagctc aggcaccaat acctgctcaa agttttcgat cagatgcccc
     3061 aggttgcggg gaaacgggcg tagataacgg agatgtgcat gcgaaacggc atatccttcc
     3121 cgttgcagct ccgcagcagc cgttttgatg gagccatagg tactgcccca tcccacgacc
     3181 agcagttttc ctttttccgg gccgctgtcc agtttctgca acggaatatg ctcggctatt
     3241 ttatccactt tttcctggcg cagcttgacc atgaggtggt ggttctccgg atcatagctt
     3301 acgttaccgg tgatattctg tttttccaga ccgccgatcc ggtgctccag ccccggtgtg
     3361 ccgggtatgg cccagggacg aacgcctctt tcatcccgca gataaggttg cagtttatcc
     3421 tctccatcac ccagttccgt tttgaattta acttctatcg ggtgaaggtg ctctgcatcg
     3481 ggaaacttcc agggttcgga tccgttggca atgtatccat cgctgagaaa gatcaccggg
     3541 gtcatatgct gtatcgcgat tcgcgccgct tcgtacacgg catcaaaaca atcactcggc
     3601 gtggaggcgg aaacgacggg catcgggctt tccccgttcc ttccgtaata ggcctgcaac
     3661 agatcgctct gctcggtctt tgtcggtagg ccggtagaag gtccgcctct ctgtatatca
     3721 caaatcaata aagggatttc cagcatcatg gccaaaccca tggcttcgga tttaagggcc
     3781 attcccggtc cggaggtact ggtaacaccc agtgatcccc cataactcgc tccgatggcc
     3841 gccgtgattc cggcgatctc atcttctgcc tggaaggtac ggatgccaaa tgctttttgt
     3901 ttgctgagct catgtaggat atccgaagca ggggtaatcg gataggtgcc gagaaagatc
     3961 ggcagcccgc ttttctgcga tgccgcgatc agtccgtatg ccagggcctg gttgcccatg
     4021 atggaacggt aggttccggg ctgcagcctg gctttggcaa ccgtataggt atgggtgaag
     4081 gtttcggtag tgtctccgaa attaaacccg gccctgagcg cgcggatatt gctttccatg
     4141 atatccgggt tcttaccgaa tttttccgtg agaaactgga tcgtgctttc cagactccgg
     4201 ttatacatcc aatataaaaa tcccaggaca aacatattct ttgcccggtc cttttcttta
     4261 ttgccaagcg caaaatcttt cagggcctcc cgggtgagtt tggtcacatc gatgcggatc
     4321 aggtcataat tacccaggct gtgatcctcc aatggattaa ttccctcggg gtaattcgcg
     4381 agccggaggt tttttgcgtc gaagccctcc acattggcaa tgatccgtcc tccctttttc
     4441 agggttttca gattggtttt caatgctgcc gcattcatgg ccaccaaaac atcacactcg
     4501 tctcccgggg taaatatatg atcgcttgaa aaatggagct gatagccgct caccccgggc
     4561 agcgtgccct ggggagcccg aatctcggcc ggaaaatcgg ggaacgtagc gaggtcaatt
     4621 cctaataagg cggtgttgtt ggtaaactgg ctacccgtta actgcatgcc atctcccgaa
     4681 tcgcccgcaa atttgatcac tacatcccgt aggattgtat ctgtaggatg tgaggacata
     4741 agtctgcaaa ggtaagaatt ctgaagaact attaattctt catcgcctta tacaccgcct
     4801 ccagaatatt tccccttacg ttcaaagacg agatcttatt gctttcaacc ggattcaccc
     4861 ggttggaaag aaagacaaag accagctgtt ttaccggatc gtcccagatc cagatcccgg
     4921 taaaaccgga atgaccgtaa gattggggag aagcggaaac gcagggataa ggatccggat
     4981 tggtcgcatt gtctttctgc ggtttgtcga atccgagccc ccggcgactg ataccgctgt
     5041 gatattcgga aaaatagtca atggtttctt ttttcaggta ccggtggcca ttgattatgc
     5101 ccccgtttat cagcatctgc aggatgacac cgaggtcata ggcgtcgctg aataatccgg
     5161 catgcccgga gacgccgcca aacatggccg cgcccggatc gtgcacatcg ccgtgaatga
     5221 gctggcggcg gaaggcggtc tccagctggg tgggggcgat acggtttaac ggaaaccggt
     5281 tgcgtggctt gaatccggta ctggtcagcc ccatgggcac ataaaaatta tcgtaagcat
     5341 attgatccag ggtcttgccg ctgatggctt ccacaatttt tcccatgaaa ataaaatcgt
     5401 tgtcgctgta aatataattt ccaacgcgac ccagcgggct ggtcagtatt cttttataca
     5461 tcgtatccag ccagtcgctg cgcatataca ggtgttccgc cacgcgaacg caatggagac
     5521 tgtcgccgac agatgaataa acggagggca ggggcaaccc ttccttatcc ttggtgatgg
     5581 tttccgcata aaagggaata aacgctttta agccggcctg gtgaagcatg atgtcccaga
     5641 tcttcagcgt atccttatcg cttccccgca cccagggcag gtaatcaccc agggttttgt
     5701 tcagatccag tttcccttcg tcatataatt tcatcaccga aatggtggtt gcgcaaatct
     5761 tggtcacgga agccaggtca taaatggttt ccggatatac cgggtctttt ttatcatact
     5821 gcatatagcc gaatgccttt tcgtaagcaa tccggccgtt ccgggcgacc aataccacgc
     5881 atcccggcgt tgcctgcaat tcaatggccc gtttacagat cgaatcaatc tccagtaatc
     5941 gggagggttc aaaaccgacc gaagccggag gggcctccgg caacaggcct cgcgtgaacg
     6001 taatgccgta accggctttc atttcagcac aaacggaaac cggtaaccgc ccccggggaa
     6061 gaaactttcc gttgagccag tctgccgcaa cggactgggt gatttcatta tcgtcgtaac
     6121 attcgagcag gttctgggca ttgcaaacat atttaatcac ataggggttt ccgaaaacaa
     6181 agctgatcac gccggattgt tgttccagct ttttcagcag gctcagggcg gccgggctga
     6241 taccgaaatc atgggcggga tagcggttca aattatgcat cccgatgatc agcatgtcgt
     6301 accggttgcg caacagttca taagctgccg ctgctttcag actgtcgagg gaataatcga
     6361 aataataaac atttgcgtgg taatcttccc ggactcgacg ggcaaattca ttgtcatttg
     6421 ccaggccgaa accgatatag gctacccgct ttcccgccat ggattcaggc agggaacggt
     6481 cgttccgcct taataaggta agcgacaggt gtgccacttt cacatacata tcccgcacgt
     6541 ctttgttgag gtcctcaatc aaatgcgtgg tatccaccgg ttgcagccgg gtaagcccca
     6601 gttgatattt tgccgccagc actttcttta cgcggtcatt caggtccttc catttcaatt
     6661 ttttttcttt taccgcctga aggatcttcg aaatgctgag ttccacatca cccggcaggc
     6721 aaagcacgtc attgccggca atcagggact gcacggaagc atctccatcc gggtaatatt
     6781 tagtcacgcc tttcatctcc agcgcatcgg taaagctcag tccggaaaat ttgagcttcc
     6841 tgcgcagcag tttggtgacg gcattgtagg aaatggaagc cggagtattg ggttttttat
     6901 caatggccgg aacaaataaa tgaccggtca ttatcccctg gaccccggca tcgattaatt
     6961 ttttaaaagg atagagttcc agggtatcca gctcgttcat cgtcttgttg atcaccggca
     7021 ggtcgtagtg cgaatccaca gacacgtctc cgtgcccggg aaaatgcttg gcgactgcca
     7081 taataccggt cgcctgaagg cccttcatat attgaatacc cagttcggcc acgcggtact
     7141 gatcctctcc gaatgaacga tcgttgatca cggggttatc gggattgttg ttcacgtcca
     7201 ccacgggggc aaaattaatc tggatgccca tgcgtttgca ttgctcccca accagctggc
     7261 cgtattgata aatgagctgc ggatcctgca cggcgcccag catcatctgg cggggaaggc
     7321 tgcccacact gtccatgcgc atgcccaggc cgttctccgc atcaatggaa aagagtatgg
     7381 gcgtcttggc gatctgctga aaatgattga tgaaggccgc ctgtgttttg gggcctccct
     7441 gaaaaagaca gatcccgccg acatcatacc ggaggatatc tttttctacc tggacattgt
     7501 taaagctgat c
//
LOCUS       DASYCU010000454         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4304169, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000454 DASYCU010000000
VERSION     DASYCU010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4304169"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VGM24_09170"
     CDS             <1..619
                     /locus_tag="VGM24_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155588.1"
                     /translation="SKPDATPYSPFKYYPVRNPGQWEIKKSGAGIRFIQRLQDESYGY
                     QYEKDLELVKGKPELLLIHRLKNAGKKTIETEVFDHNFFLLNMRPPGPGLVLSFPFDL
                     SADQARGLGDIADLRGDSITVLRQPAARESVYAELHGYGETEKDYDILLQNRITGIGV
                     RIRCDRPLSRLVFWGSSTILCPEPYIRVKVLPGETFRWTIKYDLH"
     gene            682..>1557
                     /locus_tag="VGM24_09175"
     CDS             682..>1557
                     /locus_tag="VGM24_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY4155589.1"
                     /translation="MILLLAAGCSHPEPDTEWREYGGNKAGNRYSTLSQINLRNVKNL
                     LPAWTYHSAGTGKTAAGEKDADQEIQCQPIVVKGVLYGLDPALKLFALDAATGRELWK
                     FDPFSELTPRYTRSRGMIYWEDGTDRRIFFSAGSGLYAVDAGTGKPVRSFAEAGRLSL
                     QTGLGINHDVANLYVAATSPAIIWQNILIVGSAVSEGGNAAPGYVRGFDVRTGKLVWT
                     FHTIPQPGEPGYETWPKDAYQWAGGVNNWGGMALDEKRGLVYFGTGSPSSDFYGGDRA
                     GMNLYANCILALQAQT"
BASE COUNT          355 a          414 c          436 g          352 t
ORIGIN      
        1 atccaaaccc gatgcaacgc cttattcccc tttcaaatat tatccggtac ggaatcccgg
       61 ccaatgggaa ataaaaaaat cgggtgcggg catccgcttt attcagcggc tgcaggatga
      121 gtcgtatggt tatcaatatg aaaaagatct ggaactggta aaagggaagc cggagctcct
      181 gctgatccac cgcctgaaaa atgccgggaa gaaaacgatt gaaacagagg tcttcgatca
      241 taattttttt cttctgaata tgcgtccgcc tggtccgggc ctcgtgctta gtttcccatt
      301 tgatctttcc gcagaccagg cccgggggct cggcgatatt gcggacttac ggggtgattc
      361 cattacggta ttgcggcagc ctgctgcccg tgaatccgtt tatgctgaat tacacggcta
      421 tggcgaaacg gaaaaggatt atgatatcct tcttcagaac cggattaccg gaatcggcgt
      481 tcgcatccgc tgcgaccgcc ccctgtccag gcttgttttc tggggttctt ccacgatact
      541 ctgcccggag ccctatatcc gggtgaaagt gcttccgggt gaaaccttcc gctggactat
      601 taaatatgat ctccattaac cagaacaatg gagaagggat agcttatgac aaaaattaaa
      661 attgtccgtt tttcaatctg catgatcctc ctgcttgccg ccggctgctc gcatccggag
      721 ccggatacgg aatggcgcga atatggagga aataaagcgg gaaaccggta ttcgacgctc
      781 agccagatca acctccgtaa cgtaaaaaac ctgcttcccg cctggaccta tcattcggcc
      841 ggcaccggaa aaacggcggc cggggaaaag gacgccgacc aggaaattca atgccagccg
      901 atcgtggtaa agggagtgct ttacggtttg gatccggcct taaaactctt cgccctggat
      961 gccgccaccg gcagggaact ttggaagttc gatccgtttt cggagctaac gccgaggtat
     1021 acccgaagcc ggggaatgat atattgggag gatggaaccg accggcggat ctttttttcc
     1081 gccggttccg gcctgtacgc cgtggacgca ggcacgggca agcccgtccg gagttttgcg
     1141 gaagcgggca ggctcagtct ccaaacgggt ctgggcatca accacgatgt ggcgaatctc
     1201 tatgttgccg ccacttctcc ggcaatcatc tggcagaata tcctgatcgt cggttccgcc
     1261 gtgtccgagg gcggcaatgc ggcgcccggc tatgtacgcg gcttcgatgt gcgcacgggt
     1321 aaactcgtgt ggacctttca taccattccg cagccgggcg agcccggcta cgagacctgg
     1381 ccgaaagatg cctatcaatg ggccgggggt gtaaataact ggggcggaat ggccctggat
     1441 gaaaaaagag ggcttgttta tttcggaacg ggttcgccct catccgattt ctatggcggc
     1501 gaccgcgccg gcatgaacct gtatgcgaat tgcatcctgg ccttgcaggc acagacc
//
LOCUS       DASYCU010000455         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4879662, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000455 DASYCU010000000
VERSION     DASYCU010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4879662"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VGM24_09180"
     CDS             complement(<1..282)
                     /locus_tag="VGM24_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY4155590.1"
                     /translation="MKKYIFCGYLLSVALFVQAQTVSWDSTYRPASYKQRLALFESYP
                     NSTQDIIFLGNSITYGTDWNELLQMPRARNRGISGDISFGVLQRLEEITE"
     gene            complement(374..1945)
                     /locus_tag="VGM24_09185"
     CDS             complement(374..1945)
                     /locus_tag="VGM24_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155591.1"
                     /translation="MKNKWLIILSASLGMLFSNCNKSLLNTSPNNKYVESNFWETEAA
                     AQAGLAGCYGVLNSTGMFGGDATPLFEETASPNAYNYSNTEGFNSIAEGLQSASSSGI
                     IPDRWKACYSGIGRCNTFLVKVNEVEMDDALKTRMKAEAYFLRALYYFTLQNYYGGVP
                     LVLNPPDRVSESDLPRTDREAVISQINSDLDSAAAALPLKYSGGDLGRATKGAALALK
                     ARVALFEASPLFNTGNDMARWQTAADAAQAVIDLSAGAGYGLFPDYRSLFLPANENNQ
                     EVIFDVQFIYPDLGNSFDLIDKQYNTNAPLLDMADAYDMSNGLPITDPASGYDPANPY
                     ANRDPRLYQTIVYPGDTFMTVKVTPSRFAITGFGLKKYSIYDKGAPPAGMSDLKAGQS
                     ETNYIILRYADVLLMYAEAKNEASGPDATVYDAINQVRERAGMPDITGTHSRDELREI
                     IRHERRVEFAGEGTYYNDIRRWKTAEQVLNATIYTYDHSAIETRKFLPERDYWWPVPQ
                     TQLDLNPNLEQNQGY"
     gene            complement(1975..>2475)
                     /locus_tag="VGM24_09190"
     CDS             complement(1975..>2475)
                     /locus_tag="VGM24_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155592.1"
                     /translation="PSSTVIPKYTYGFGLNFNYKGISLDAFFQGVAGIKVYPTANMAF
                     PFNNGADATWEWATDSWTPDHPNARLPIVTESTAGQDNFQNSDFWMRDGSYLRLKDIQ
                     LAYELPGKWLSRAKISKLAVYINAENWVTFSKYRDFDPESIVNANSLYHYPMLKTLTG
                     GVKVTF"
BASE COUNT          591 a          628 c          611 g          645 t
ORIGIN      
        1 ttcagttatt tcctccaaac gctgcaatac accaaaactg atatcgccgg aaataccgcg
       61 attccgcgcc cggggcattt gcagcaactc attccagtcg gttccgtagg tgatgctgtt
      121 gcccagaaag ataatgtcct gcgttgaatt gggatagctc tcgaagaggg ccagccgctg
      181 cttataggaa gcgggacggt aggtactgtc ccaggagacc gtttgggcct gcacaaaaag
      241 ggcaactgaa agcaggtacc cgcagaagat atattttttc atgttcctga tattattcat
      301 aaaattccga cccgggaaat cttcccgaag gatagcgggc cgtgccgccc gggtcacgac
      361 ctgctcttcc tttctaatag ccctgattct gttccagatt cgggttcaga tccagttgcg
      421 tttgcggtac cggccaccag taatcccttt ccggaagaaa cttccgggtt tcgatggcgc
      481 tgtgatcata ggtgtagatg gtggcattca gaacctgttc tgctgttttc catctgcgaa
      541 tatcattgta atacgttcct tctccggcaa attcaacccg gcgttcatgc cggatgatct
      601 cgcggagctc gtcccggcta tgcgtacccg ttatatccgg cataccggct ctttcccgta
      661 cctgatttat cgcatcgtaa accgttgcat ccggtccgct ggcctcgttc ttagcttctg
      721 catacattaa caatacatcg gcatatcgca gaatgatata gttggtttct gactggccgg
      781 ctttcaggtc actcattccc gcaggaggag cgcctttatc gtagatgcta tattttttca
      841 agccaaagcc ggtgatggca aaacgggacg gcgttacttt aaccgtcata aacgtatcgc
      901 cgggatatac tatggtctga tataaacggg ggtcgcggtt tgcataagga ttggcgggat
      961 catagcctga agccggatcc gtaatgggca acccgttgct catgtcgtaa gcatccgcca
     1021 tatccagcaa gggagcgttt gtattgtatt gcttgtcgat aaggtcgaat gaatttccca
     1081 ggtccgggta aatgaactgc acatcaaaaa tgacttcctg gttattttca ttggccggca
     1141 gaaacaagga acggtaatcg ggaaacagac cataacccgc gccggcggaa aggtcaataa
     1201 cagcctgcgc ggcatccgct gcggtctgcc agcgagccat atcattaccg gtgttgaaaa
     1261 gcgggctggc ttcaaacagc gctaccctgg cctttaatgc cagtgccgct cctttggtgg
     1321 ccctgccgag atctccgccg ctatacttca gcggaagcgc cgccgcagcg ctgtccagat
     1381 ctgaattgat ttgggaaatt acggcttccc ggtccgttct cggcagatca gactcggaaa
     1441 cccggtcagg tgggttcaat accaggggaa cgcctccata ataattctgc agcgtaaaat
     1501 aataaagggc acggagaaaa taagcttctg ccttcattct tgtcttcagg gcatcatcca
     1561 tttccacttc gttcactttc accagaaaag tattgcaacg tccgattccg gaatagcagg
     1621 ctttccaccg gtccggaata attccggaac tgctggcgga ttgcaagcct tcggcaatcg
     1681 aattaaatcc ttcggtattg ctgtaattat acgcattggg agatgccgtt tcttcaaaca
     1741 ggggcgtggc atcgccgccg aacattccgg tactgttcaa tacgccgtaa caaccggcca
     1801 gtccggcctg tgccgccgcc tcggtttccc agaaattgct ttccacgtat ttattgttag
     1861 gggaagtgtt cagcaggctc ttattgcagt tactgaacag catgcccagg ctggcagaaa
     1921 gtatgattaa ccatttattt ttcatctgac tgtatttagt tattagtaga tcgtttagaa
     1981 agtgacttta actccgccgg tgagggtttt taacatcggg tagtgataga gtgaattcgc
     2041 attcactatg gactccggat caaaatccct gtatttggaa aaagtgaccc agttttccgc
     2101 attgatatat acagccagct tgctgatctt cgcgcgggaa agccacttac ccggcaactc
     2161 gtaggccagc tggatatctt ttaaccgcag gtaagaacca tcccgcatcc agaaatcaga
     2221 gttttgaaaa ttatcctgac ccgccgtgct ttccgttacg ataggcaaac gcgcattcgg
     2281 atggtcgggt gtccaggagt ccgtggccca ttcccaggta gcatctgccc cgttattaaa
     2341 agggaaagcc atattggccg tcgggtatac ttttataccg gcgacgccct ggaaaaaagc
     2401 atcgagtgaa atgcctttgt aattaaaatt cagcccaaag ccataggtat attttgggat
     2461 aaccgtggaa cttgg
//
LOCUS       DASYCU010000456         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1112496, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000456 DASYCU010000000
VERSION     DASYCU010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112496"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VGM24_09195"
     CDS             complement(<1..1163)
                     /locus_tag="VGM24_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4155593.1"
                     /translation="MKKIYLSLLAVSIAFRISAQTDSAKINPLDEVVVTASKTNIKQS
                     QTGKLVTVIDSKTIQNNAGHTLTELLNNQAGFMMIGSSNTMGSNVENYFRGAESGNLL
                     IVIDGIPVSDPSQISNTFDLNSIPLEQIDRIEILKGGQSTIWGSDAVAGVVQIFLKKE
                     SKKPLSANGNFSYGTYNSLRAGAGVNGTYRKLGYRLQYNYQKSDGFSNAYDSTGNNGF
                     DKDGFSQNNLQAELKFKFSDLFNMKLLGTLGDYHFVLDEGAFTDDKDYGGKNINDLGA
                     VYFNYHKNNLTWNTLASYQHAKRYFTDDSTDRSSPYAYYSHGEYLSRNITAESYVNLR
                     FAEHMQLVSGVQFLDQNTDQSYLSLSDFGPFFSSLGGDSAHIKQYSAYASYLIT"
     regulatory      complement(1296..1500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1528..>2885)
                     /locus_tag="VGM24_09200"
     CDS             complement(1528..>2885)
                     /locus_tag="VGM24_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4155594.1"
                     /translation="GDLYYQTPGALNITQYRVNPKEARQAAGGFPSADQAKAAIYQKT
                     FMAALSNTQHFNEHWQNRTNFYGAYTNYLNPTFLNYEIRNEPHFGGRTVFTYQSDRFQ
                     LNAGGEAQKGFFKTEDFGNTGGSPDTVQTNDNINTWTYSLFAQADLEFARNWQVTAGA
                     SFNKSFIGIHRLSVPGFVPRNKTFSNEIAPRLAVSRKISRDLLVYASVSKGFSAPAVS
                     EVLPSNQSINTDLQPEQGICYEAGVKSGFLNQRLYAEVVGFYFQLQKAIVVRKDASGS
                     DYYINAGATRQEGIESQLSYQLISSPGPVLTSARLWLSYTLDRFTYQDFKKGTVDYSG
                     KHLPGVANNTVAGGLDLFSTAGIFLLLTYYYSDRLPLDDANTAYADSYQLLGGKIGYK
                     TNAIKRINLSVFAGVDNAFNTKYSLGDDVNAAAGRYYNAAAGVNYFAGLAISYNRPKR
                     S"
BASE COUNT          697 a          693 c          728 g          767 t
ORIGIN      
        1 gtgatcaggt aagaggcata ggcggaatac tgtttgatat gggcgctgtc tcctccgaga
       61 ctgctgaaaa agggtccaaa atcgctcagg ctgagatagg actgatccgt gttctggtcc
      121 aggaactgta ctccgctaac caattgcatg tgttcggcga accggaggtt cacataggat
      181 tcagccgtga tgttccggga taaatattcg ccatgggaat aataggcata agggcttgac
      241 cggtccgtac tatcgtccgt aaaatatctc tttgcgtgct ggtaactggc cagggtattc
      301 caggtaaggt tgtttttatg ataattgaaa tagacggccc cgaggtcatt gatatttttt
      361 cccccgtaat ctttatcgtc cgtaaaagcg ccctcgtcca ggacaaaatg atagtccccc
      421 agggtcccca gcagtttcat attgaacagg tctgaaaatt tgaatttcag ttctgcctgc
      481 agattgtttt gcgaaaaacc atctttgtca aatccgttgt tcccggtact gtcgtaggca
      541 ttggaaaatc catcgctctt ctgataattg tattgaagcc ggtatcccag tttccgataa
      601 gtcccgttaa cgcccgcacc ggcgcgcagg ctgttgtagg ttccgtaact gaaatttccg
      661 ttggccgaca agggcttctt actttctttt ttcaggaaga tctgtacgac accggcaacg
      721 gcgtcactgc cccagatcgt gctctgccct cccttcagta tttcaatccg gtcaatctgt
      781 tccagcggaa tgctgttgag atcgaaggta ttgctgatct gggaagggtc tgaaaccggg
      841 atgccgtcga ttacgatcag caggttgccg gactccgcgc cgcggaaata attttccacg
      901 ttggatccca tggtattgct gctgccgatc atcatgaacc cggcctggtt attcagcaat
      961 tccgtaagcg tgtgcccggc attgttctga atggttttgg aatcgatcac cgtaaccagc
     1021 tttccggttt gcgattgctt gatgtttgtt ttggatgccg ttaccaccac ttcatccagt
     1081 gggttgatct tggcggaatc cgtttgtgcg gagatccgga aggcgatgga taccgccagc
     1141 agggacaaat aaattttttt catgctcatt cttcgtttta aatgatttgg ttaagaagaa
     1201 cgagctttcg atccatgaaa taagtagaaa tgtaagttct gaaaacaggt cctgtttaca
     1261 aaaccagcat ctccggcctt tcacccgaaa gctcagtgat aataaattca cctggcaggt
     1321 cttctggctt gttccctgtt taccgccttc ccgttccgtt ctaccggaac agtggccgca
     1381 gtgtaaacaa tttcaggctc atcgcctgca gaactcacag ctacggggat agcgccggtt
     1441 ttacaccgga cttccctttt aaccccgacc catgcgggga ccagatttgt gcaaatgtaa
     1501 tggagtggaa taaattaaga aaagaaatta tgagcgcttt ggccggttat atgaaatggc
     1561 cagtccggca aaataattaa cccctgccgc cgcattgtaa tagcggccgg cagccgcatt
     1621 gacgtcatcc cccaaactgt atttggtatt gaaggcgttg tccactccgg caaaaacaga
     1681 caggttgatt ctcttaatgg catttgtttt ataaccgatt ttgcccccaa gcaattgata
     1741 ggaatccgca taagccgtgt tggcatcgtc gagtggaagg cggtcgctgt agtaataggt
     1801 caggagcagg aaaatgccgg ctgtactgaa caggtccagg ccaccggcca cggtattatt
     1861 tgcaacgccc ggtagatgct ttccggaata atccactgtg ccctttttaa aatcctggta
     1921 ggtaaaccgg tccagggtat aggaaagcca gagacgggcg ctcgttaaga cgggacccgg
     1981 cgaagaaata agctgatagg ataactggga ttctattccc tcctgacgcg tagcgccggc
     2041 attgatgtaa taatcagaac cggaagcgtc tttccgcacc acaatggctt tctgtaattg
     2101 aaaataaaat ccaaccactt cggcgtacag acgctggtta agaaaaccgg atttcactcc
     2161 ggcctcataa cagatcccct gttccggctg cagatccgtg ttgatgcttt gattggaagg
     2221 gagtacttcg gataccgcag gtgctgaaaa tcctttcgaa acgcttgcgt atacgagcag
     2281 gtcccggctg attttcctgg aaacagccag gcgcggagcg atttcgttgc tgaatgtttt
     2341 attgcgcgga acgaacccgg gaacggacag acgatggatc ccgatgaagg atttattaaa
     2401 actggcgcct gccgtgacct gccagttccg ggcaaactca agatcggcct gcgcaaaaag
     2461 ggaataggtc caggtattga tattgtcgtt ggtctgaacc gtatccggag atccgccggt
     2521 attcccgaaa tcttccgttt tgaaaaatcc tttctgcgct tctccgccgg cattcagctg
     2581 aaaccggtca gactgatacg tgaaaacggt ccttcctccg aaatgcggtt cattgcggat
     2641 ctcataattc aaaaaagtag ggttgagata gttggtatag gcgccgtaaa aattcgtgcg
     2701 gttctgccag tgttcattga aatgttgcgt gttcgaaagg gcagccataa aagtcttctg
     2761 ataaattgcc gcctttgcct ggtctgccga aggaaagccg ccggcagcct gcctggcctc
     2821 tttcgggttg accctgtatt gagtgatgtt cagtgcaccc ggtgtttgat aatacaaatc
     2881 gccat
//
LOCUS       DASYCU010000457         3889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_971967, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000457 DASYCU010000000
VERSION     DASYCU010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971967"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VGM24_09205"
     CDS             complement(<1..324)
                     /locus_tag="VGM24_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY4155595.1"
                     /translation="MDSIAVLLLAGFIVGALGTLIGAGGGFILVPVLIIIHPEWSPEI
                     ITAVSIAIVAANAISGTYAYARSGRIDYKAGIIFALFTIPGSILGVYTTSYIPKTAFH
                     IIFGIL"
     gene            591..1130
                     /locus_tag="VGM24_09210"
     CDS             591..1130
                     /locus_tag="VGM24_09210"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEY4155596.1"
                     /translation="MKRASFTKAIVLTLAGVFMTSLIWAQGDKSKRPSPPETASGMAG
                     GATVTINYSSPSVKGRKIWGGLVPYDKVWRAGANEATIFETNKAIKVEGKDLPAGKYS
                     LFATPGEKEWTIILNSETGQWGIKRTGEANRDPAKDVVTVMVKPKKSSSMQEHLTYKV
                     SSPGFILSWENIEVPVSIK"
     gene            complement(1221..1667)
                     /locus_tag="VGM24_09215"
     CDS             complement(1221..1667)
                     /locus_tag="VGM24_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY4155597.1"
                     /translation="MDVKPAIRAKPLILEKTLPAPANRVWQALTDSSQMRKWYFDIPE
                     FKPEPGFEFRFTAGSESKTYVHLCKVTEVIPGRKIAYTWRYQDCPGESEVSFELFPEG
                     SNTRVRVTHSGLESFPQDSKDFAKESFTEGWNQILGTSLWKFVEAK"
     gene            complement(1780..2619)
                     /locus_tag="VGM24_09220"
     CDS             complement(1780..2619)
                     /locus_tag="VGM24_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HEY4155598.1"
                     /translation="MILVADSGSTKTAWGLAYENGERLLFDTEGYNPYYVDKEYIIRS
                     LNKNLPRSFNTDQVKEVHFYGAGCMLDRTPVMEEALLAVFQKAEINAAMDLVGAARAL
                     LGRSAGFAAILGTGTNTCIYNGDGIAHYIDSLGYMLGDEGSGTYIGKKLIQGYLRGYM
                     PAEVSEKFAGYANLSFDDIMDRVYNRGLANRFCAGFAQFAGNYIEMPYVYELVKNSFI
                     DFFSSLVSRYPDYQQYSFNCVGSVGYHFKTILESVCSEFGMPVGKIMASPMEGLLQYH
                     SRK"
     gene            complement(2635..3876)
                     /locus_tag="VGM24_09225"
     CDS             complement(2635..3876)
                     /locus_tag="VGM24_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEY4155599.1"
                     /translation="MNPLIIIGALFFIFGFITWISAVLIPYLQIACELNNFQSYLVAF
                     AFYISYFAMALPSGWVLKITGYKKGISFGLLLVAAGSVIFIPAAVYRVYGIFLAGLFI
                     QGAGLALLQTAANPYVSILGPVESGAKRMSIMGICNGIAGILAPLILGAVILENADEL
                     VAGLGRMNGSEKTGALNALAHKVILPYLIIAGLLVLLAIIINRLSLPELDPPPEDEAD
                     GPADQRKTSIFQFTHLIIGVFALFLYVGVEVIAGNTIIGYGTYQHIPLSIAKFFSSFT
                     LTGMLAGYLVGIICIPRYFSQQSALKYSAMAGIIFVLLALVTRGLTSVVFIALLGLSN
                     SLMFPSIWPLAIDGLGKFTKTGAALLVMAISGGALLPLLYGWLADHFNARQAYWMVIP
                     CYLVIGYYAFAGYKAGRKRII"
BASE COUNT         1095 a         1002 c          887 g          905 t
ORIGIN      
        1 caggatgccg aaaattatat gaaaggccgt tttcggaata taggacgtcg tgtaaacccc
       61 gaggatggag cctggaatgg tgaagagggc aaaaataatc ccggccttat aatctatgcg
      121 cccggaacgg gcataggcat aggtaccgga aatggcattg gccgccacaa tggctatgga
      181 aacggccgtt atgatttcag gagaccattc cggatggatg atgatcagga ccggaaccag
      241 gatgaaaccg ccgcctgctc ctatcagtgt tcccagcgcg ccaactataa atccggccaa
      301 caagagtact gctatgctat ccatatatcc gtccggcttt aattgccgga ataatcggta
      361 aaactaggga tcagttgtct actcattgag tcttttaata aaagcaggtc tcccacccac
      421 atccgaggga attcccgatt cctattggca taattttagc ttaaaacggg tgtacggccc
      481 ggacttccga agccgagttc tatatccgcc cggcagactt caacttttcc aattccctga
      541 ctttacgaag gcaacgagac attattattc aacaattaaa caatagatat atgaaaagag
      601 caagctttac aaaggccatc gtattgacgc tggccggcgt cttcatgacc tcacttatct
      661 gggcccaggg agataaatca aaaagaccga gccctccgga aacagccagc ggaatggcgg
      721 gcggagcaac ggtcacgatc aactacagca gtccttccgt taagggaaga aaaatctggg
      781 gcggacttgt cccctatgat aaagtatggc gtgccggggc aaacgaagcc accattttcg
      841 aaacgaataa ggccattaaa gtggagggca aggatttacc tgccggcaaa tacagccttt
      901 ttgccactcc gggagaaaag gaatggacta ttatcctgaa ttccgaaacc ggtcaatggg
      961 gaattaaaag aaccggtgag gccaaccggg atccggcaaa agatgtggta acggtcatgg
     1021 ttaaacctaa aaagtcttcc agtatgcagg agcacctgac ctataaggtc agtagccccg
     1081 gctttatact cagttgggaa aatatagaag ttcccgtttc tattaaataa atccttcgct
     1141 cctgaaataa agtttccgtt tttgaaaaag cctgccggaa aaggacctta tcttttccgg
     1201 caggcttttt actttcgcca ttatttagct tccacgaatt tccacagcga agttcccagg
     1261 atttgattcc agccttcggt aaaactttct tttgcaaaat ctttgctgtc ctgcggaaaa
     1321 ctttccagcc cggagtgcgt aacccttacc cgggtattac ttccctccgg gaaaagttca
     1381 aagctcactt ccgattctcc cggacaatcc tgatatctcc aggtgtatgc gatcttcctg
     1441 cccggaatca cttccgttac cttgcacaga tgcacgtacg tttttgattc cgatcccgcc
     1501 gtgaaccgga attcgaaacc cggctccggc ttgaactccg ggatatcaaa ataccatttc
     1561 ctcatttgac tgctgtctgt aagagcctgc cagacccggt ttgccggcgc cggcaatgtt
     1621 ttttccaaga tcagcggttt ggcgcggatc gctggcttta catccataat gatgtagttt
     1681 aagaatgaat aaacaggaaa cctttcggtt gctaatgtag gaaaataatc ggcaggcccc
     1741 tgaaaaaagc ccgcgggaat gatcatttta gggaattgtt cacttccgcg aatgatactg
     1801 cagcaatcct tccatcgggg aggccatgat tttccccacg ggcatgccga actccgagca
     1861 tacggattcc aggatggttt taaaatgata gcccacggaa cccacacaat tgaatgaata
     1921 ttgctgataa tccggatacc ggctcaccag gctgctgaaa aaatcgataa atgaattttt
     1981 gaccagctca tatacatagg gcatctcaat ataattcccg gcaaattgcg caaaaccggc
     2041 acaaaaacga ttggccagac cccggttgta aacccggtcc atgatatcat cgaaagacag
     2101 gttggcgtag ccggcgaatt tttcactgac ttccgccggc atataacccc gcagatatcc
     2161 ctggatcaat tttttaccga tatatgttcc gctaccttca tcccctaaca tatatccaag
     2221 ggaatcaata taatgtgcta ttccatctcc attataaata caggtattcg tacccgttcc
     2281 caggatagcg gcaaatccgg cgctccgtcc gagaagcgcc cgggcagctc ccaccagatc
     2341 catggctgca tttatttccg ccttctgaaa taccgccagc aaggcctctt ccattaccgg
     2401 ggtcctgtcc agcatacaac ccgctccata gaagtgaact tccttcacct gatccgtatt
     2461 aaaagagcgg ggaagatttt tattcaggga ccggataata tattccttgt ccacataata
     2521 tggattatac ccttccgtat caaaaagcag tctttctcca ttttcataag ccaggcccca
     2581 ggctgttttt gtcgatccgc tgtcagcaac taatatcata tctctgttta aaaattaaat
     2641 aattcgtttt ctcccggctt tatagccggc aaaagcataa taaccgatca ccagatagca
     2701 gggaatgacc atccagtaag cctgccgggc attaaaatga tcggccagcc agccatacaa
     2761 taagggtaac agggcgcctc cggatatggc catcaccagg agagcagcgc ccgttttcgt
     2821 aaattttccg agcccgtcaa tagccagtgg ccagatcgaa ggaaacatta aggagttgga
     2881 cagacctagc agggcgatga atacgactga agtcaggccc cgggtcacca gggccagcaa
     2941 gacaaaaatg atccctgcca ttgccgaata cttcagggcc gactgctggc tgaaatatct
     3001 tggaatacat ataatcccga ccaggtatcc ggccagcatt ccggttaaag taaaagaaga
     3061 gaaaaatttc gcaatggaaa gaggaatatg ctgatatgtt ccataaccaa taatggtatt
     3121 acccgcaatc acctccaccc ccacatatag gaacaaggca aaaacgccga tgatcaggtg
     3181 ggtaaattga aaaatgctgg tcttccgttg atccgccgga ccgtccgctt catcttcggg
     3241 cggtgggtcc agttcgggca gcgacaggcg attaatgatg atggctaaaa gaaccagcag
     3301 cccggcgatg atcaggtagg gaaggatcac cttatgagcc agggcattca atgctccggt
     3361 tttttcgctc ccgttcatcc ggcccaatcc ggccactaac tcatccgcgt tctccagaat
     3421 gaccgctccc aagatcagcg gggccaggat cccggcaatt ccgttgcaga tgcccatgat
     3481 gctcatccgt ttcgcaccac tttccaccgg tccgaggatg ctgacataag ggttggccgc
     3541 tgtctgaagc agggccagcc cggccccctg gataaataat ccggcaagaa aaattccata
     3601 gacccggtat actgcggcag gaataaaaat aacagacccg gcggcaacca gcaataaacc
     3661 aaaggatatc ccttttttgt atccggtaat tttcaatacc cagcccgacg gcagcgccat
     3721 cgcaaaatag gagatataaa atgcaaaggc caccaggtag gactgaaagt tattcagttc
     3781 gcaggcaatc tgcagataag gaattaatac ggcactgatc caggtgataa agccgaatat
     3841 aaaaaaaagc gccccgatga tgatcagggg attcatgccc gcccccggg
//
LOCUS       DASYCU010000458         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2997328, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000458 DASYCU010000000
VERSION     DASYCU010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997328"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VGM24_09230"
     CDS             <1..457
                     /locus_tag="VGM24_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155600.1"
                     /translation="SDASWSTMLLQWKGYYRFPANTPNILALWSFNWFTLSGKPPYLM
                     LPSTGWDKSYNTGRGYIQGRFRSNNMLDAEAEYRIRLTKNGLLGMVLFGNLQSFSEVD
                     SYRFESAAPAGGLGLRIKLNKYSRTNISIDYGWGRQGSRGFFVNLGEVF"
     gene            complement(473..1468)
                     /gene="mgrA"
                     /locus_tag="VGM24_09235"
     CDS             complement(473..1468)
                     /gene="mgrA"
                     /locus_tag="VGM24_09235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEY4155601.1"
                     /translation="MSIAFREDRYDKMEYRRCGRSGLKLPAVSLGLWHNFGDIDNQQN
                     ARDILRLAFDRGITHFDLANNYGPPPGSAEENFGRIFKQDFKPYRDELILSTKAGWGM
                     WPGPYGDYGSKKYLVASLDQSLKRMGLEYVDIFYHHRPDPATPLEESMSALDLIVRQG
                     KALYVGISSYSADETAKAVKILRDLGTPCLIHQPKYSMFERWIEDGLLDVLKKEGVGC
                     ICFSPLAQGMLTDKYLGGIPEGSRAGSHRGNGAIDESAVTMEKIEKVRKLNEMAKQRG
                     QSLAQMALSWVLRDPAVTSVLIGVSKPAQLTDSIRCLDNYRFSEEELKKIDLLLR"
     gene            1541..3460
                     /locus_tag="VGM24_09240"
     CDS             1541..3460
                     /locus_tag="VGM24_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HEY4155602.1"
                     /translation="MFLVRVLFCLLIAPVMLFAQPGAGKDSILIKSGFDNELLLGEPF
                     FLVDPGNTQSFSQISSDSFSLSAQRFLEQIKNHAGPNRYWIKFSLKNLSGSTLDLHLY
                     PGYTDYADLYFISGTNAAVHLERGHLRPQDAGDGLADQTAGTLPLSLHPHQQGIVYLG
                     LRQRSGEFYFKAIEVLDKNHLDALVMQDYDADTRFLFFQALFQGFLLCQLLYVFFQWL
                     LIRRNEYLYYFFYLLLIALYFLSKYEPLFGINLLFTKFPIFRIYLSKTFLILPYYFYF
                     RFVRSFLEMPTRYPELNRWIVPLEYFLLLYSIFDFLFIVISLNQEMQALISTFVLVAV
                     FIISACFILYMFRRRKALVYYILSGSFFVGLGSIIGTILTFLENNEHIDMGVSDIMVF
                     PQAGVILEICCFTAGLSYKSQASEKEKIQGQEKLIEQLKANELLQTRLQQIRNKIAQD
                     LHDDIGSTLSSISILSDLAMKEKDSVQTIATMKEINTHSIQLMERMDDIVWSINPKND
                     TLENLMMRIRHFATTLFEARNIDYRIRIQPDINQVKIPLDYRQHIYLVLKEAINNLVK
                     YSGATESGIHIWFDEQKLSMEVTDNGKGFDVSVQYPGNGIYGMQNRAGSMNAELEIRS
                     GPETGTTLLLTVPFI"
BASE COUNT          858 a          898 c          803 g          931 t
ORIGIN      
        1 atcggatgcc agttggagca ctatgctcct gcagtggaag ggctattacc gttttccggc
       61 aaacacgcca aacattctgg cactctggag ttttaactgg tttaccctga gtggcaaacc
      121 accttacctg atgctgccca gtacgggctg ggacaaatcc tacaataccg ggcggggcta
      181 tatccaggga cggttcagaa gcaataatat gctcgatgcg gaagccgaat accggatccg
      241 gctgacaaaa aacggattgc tcggcatggt cctgttcgga aatctgcaga gtttttccga
      301 ggtggacagt taccggtttg aatcggcagc acctgccggc gggctcgggc tgcgaattaa
      361 gctcaataaa tattcacgca ccaatatctc cattgactat ggatggggac ggcaggggtc
      421 gcggggattt tttgtgaatt taggtgaagt gttttgagcc ccccggtaat catcagcgca
      481 gcagcaaatc aatctttttc aattcttctt cgctgaagcg ataattatcc aggcaacgga
      541 tcgaatccgt cagttgtgcc ggtttgctta ctccaatcaa aaccgaagta accgccggat
      601 cgcgcaacac ccaggacaag gccatctggg caaggctctg gccccgctgt ttggccatct
      661 cattgagctt acgcactttt tcgatctttt ccattgtgac ggcagattca tcgatggctc
      721 cgttcccgcg atgacttccg gcacgggacc cttccggaat gccccccaga tatttatccg
      781 tcagcattcc ctgcgccagc ggggaaaagc agatacaacc cactccttcc tttttcaaaa
      841 cgtccagcaa tccgtcttcg atccatcttt caaacatgga atatttcggt tgatgaatca
      901 gacagggagt gcccaggtca cgcagtattt taaccgcttt tgccgtttca tccgcagaat
      961 agctggatat gccgacatac agtgccttac cctggcgaac gatcagatcc agcgcagaca
     1021 tgctttcttc caatggcgtt gccggatccg ggcggtgatg gtagaaaata tccacgtact
     1081 ccaatcccat tcttttcagg ctttgatcca ggctcgccac caggtatttt tttgaaccgt
     1141 aatccccata gggccccggc cacatccccc accccgcttt ggtggaaagg atcagttcat
     1201 cccggtaggg tttaaaatcc tgtttaaaaa tccttccgaa attctcttca gccgatccgg
     1261 gaggaggacc gtaattattt gccaggtcaa agtgggtgat cccccggtca aacgccaggc
     1321 ggagtatatc gcgcgcattc tgctgattat cgatatcgcc gaaattatgc catagcccaa
     1381 gggaaacggc gggcagtttc aatccgctgc ggccgcagcg gcgatattcc attttatcat
     1441 agcggtcttc ccgaaaagca atgctcatag ttaaaataag taggtgaacc gcaaagatgg
     1501 tttatttacc ttttctatat acatttaaag aaatattttc ttgtttctcg tccgcgttct
     1561 tttttgcctg ctgatagcgc cggtaatgct tttcgcccaa cccggggccg gcaaagattc
     1621 cattcttata aaatcaggat tcgataacga gttgttgctg ggagagcctt tctttctggt
     1681 ggacccgggc aatacccaat cattcagcca gatctcctcc gattcgtttt ctctttctgc
     1741 gcagcgtttc ctggagcaga taaaaaacca cgccggccca aaccggtact ggataaaatt
     1801 cagtctgaaa aatctgtccg gctcaaccct ggatctgcac ctgtacccgg ggtacacgga
     1861 ttatgccgat ctctatttta tttccggaac gaatgcggct gtacacctgg aaagagggca
     1921 tctgcgcccg caggatgcgg gagatgggct ggcggatcaa acagcaggca cccttcccct
     1981 ctctctccat ccacatcagc agggcatagt ttatctcggg ttgcggcaac gcagcggcga
     2041 gttttatttt aaagcgattg aagtgctgga caagaatcac ctggatgcgc tggtcatgca
     2101 ggattatgat gcggataccc gtttcctttt ttttcaggcc ttattccagg gatttctgct
     2161 gtgccagctt ctatacgtgt tctttcaatg gctgctgatc cggcgaaatg aatacctgta
     2221 ctattttttc tacctgctgc tgatcgccct ctattttctc agtaaatatg aaccgctttt
     2281 cggaataaac ctgcttttta caaaattccc catcttccgg atctatctgt ccaaaacctt
     2341 tctcatactc ccttattatt tctatttccg gtttgtccga agtttcctgg aaatgcccac
     2401 ccggtacccg gaactgaaca gatggatcgt gccgctggaa tatttcctgc tgctctattc
     2461 gatattcgac ttccttttta tcgtcatcag cctcaatcag gaaatgcagg ctctgatctc
     2521 caccttcgtt ctcgttgccg tttttattat ttccgcctgc tttattctgt acatgttccg
     2581 gagaagaaaa gcgctggtct attatatact ttccggaagt ttttttgttg gtttgggcag
     2641 catcatcgga accattctta ctttcctgga aaataatgaa cacatcgata tgggcgtctc
     2701 cgacatcatg gtctttccgc aggccggcgt tatcctggaa atatgttgtt ttacggcagg
     2761 tctcagttat aaaagccagg cctccgaaaa ggaaaagatc cagggccagg aaaaactgat
     2821 cgaacagcta aaagcaaatg aattgcttca gactaggctt cagcagatcc ggaataaaat
     2881 tgcacaggac ctgcacgatg atatcggttc cacgctcagc tcgatctcga ttctcagcga
     2941 tctggcgatg aaagaaaagg attccgtgca aacaatagcc acgatgaaag agatcaatac
     3001 ccattccatc cagctgatgg agcggatgga tgatatcgtc tggagcatta atcctaaaaa
     3061 cgataccctg gaaaatctga tgatgcggat caggcatttt gcgaccacgc tttttgaagc
     3121 ccggaatatc gactaccgga tcaggatcca gccagatatt aatcaggtaa agatcccact
     3181 ggattaccgc cagcatatct acctggtctt aaaggaagcc atcaataacc tggtaaaata
     3241 ttcaggggcc acggaatccg gcatccatat ctggtttgac gagcagaaac tttccatgga
     3301 agtgacggat aatggcaaag ggttcgatgt ttccgttcaa taccccggaa atggaattta
     3361 cggtatgcaa aaccgggcgg gatcgatgaa cgcggagctg gaaatccggt ctgggcccga
     3421 aacagggacc acccttttgc ttaccgtacc ctttatataa atcagatcat tatccgatct
     3481 ccgcattcaa
//
LOCUS       DASYCU010000459         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2417558, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000459 DASYCU010000000
VERSION     DASYCU010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417558"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VGM24_09245"
     CDS             <1..789
                     /locus_tag="VGM24_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155603.1"
                     /translation="DGLRYNWPLSTSEAKFTDRVYMPGSDLMMGMYTGHFGAGYEYPS
                     LVATWQHTGPDLSVFVHRGDQRSASISLYNFGAAKSILLKTWQLEPGIYILRQAPDRN
                     DDGIPDEAGTEQKIELKERVNAIGLTIPSKKETLLTITQLKSYGPADADLADAALSAD
                     DIRFPDTAMAAGQLSRIQCKIHNIGSRAAENLIIECSVDHKKTGQRTIPVLEAPNDLK
                     PRWTTVDFMWKALPGEHRIGIKIISAQREITKQNNEAESVVRVR"
     gene            850..1659
                     /locus_tag="VGM24_09250"
     CDS             850..1659
                     /locus_tag="VGM24_09250"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY4155604.1"
                     /translation="MDIVDAEQKDFFRENGYLVIENILTEEEILFYRNIYEEFLSNRI
                     DASRYRSDLGAHAGEDAKAPRGERITQIMLVSRLMPSILDLPLHHKTAAIAKQLLGED
                     MELDFDMLIDKPPNSATATPWHQDCAYWISMPDTRAASCWTALDDATKENGGMWYVAG
                     SHHLPVRSHRPAGKGGGALECDATEAEATAVEIKAGSCIWHHGGTIHYSRGNTTGLRR
                     RAFITNFRPAEMIAYERLRGFDHTGERAVRDDRAAVENGGPRGPFPEGAPQ"
     gene            1762..>3750
                     /locus_tag="VGM24_09255"
     CDS             1762..>3750
                     /locus_tag="VGM24_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HEY4155605.1"
                     /translation="MKFGLGTPAMMIVFLLPGVAGKATAASSSGIHSLAAVPVLPLPQ
                     HIETYRTESGPPVTAGSLLGKGLTPAAKERLLFHWSGFLKEFAAGGPAQEKPDVVEIG
                     ILGKDGSFDKAVAQWASPWSGRIGKEGYLLILRNRKRFIAAHTETGLFYGIQSLKQLI
                     RAGWKGDLFIADWPSFETRVIYDDISRGPISTVAYIKKQIERMAEIKINYLSFYIEHI
                     VQPRSHPDFAPPDGKLTTADITELSAYAAKFHMQLIGSFQSFGHFEKILSLPQYASMG
                     ATSTLISPTDPRAKQFLEEVIGELCGAFNAPWFNVNCDETFDLGQGSTKPLVDSLGAV
                     AVYASHLRFLYDIVKRHGKKMMMWGDFAVKHPEVLDRLPRDIVYLTWEYGDQSSFDHW
                     IKPFANRHLNYMICPGILNSYRMFPDMQLARANITGFTKAGHGQAAKGVITTVWDDGA
                     VYLFSSDWYGVYVAAERSWTVDSTAVPSFNTRYEQTAYGTRNGDYVKALFTLMELKKL
                     PLTYDLSDRVWQQKILPDSGKKLIVNNTGVVEAGRIVGEAEHYIRSSDPKRNQTDIST
                     LANRIALYKLILDTRLKTVAIAADYTSAAAGTPQQAGVLFTRDIKETGDLIQAYTASR
                     ETFRQAWLKENQHYWLDSALTAYDKKINRLTRLRETLKQ"
BASE COUNT          952 a          946 c         1050 g          802 t
ORIGIN      
        1 gacggacttc ggtacaactg gcccctgtct accagcgaag cgaaattcac cgaccgggtt
       61 tatatgcccg gaagtgattt gatgatgggc atgtataccg gtcatttcgg ggccggatat
      121 gaatatcctt ccctggtggc cacatggcag cacacgggtc cggacctttc tgtgtttgtg
      181 caccgggggg atcagcggtc cgccagcatt tctctttata atttcggtgc cgcgaaatcc
      241 attttgttga agacctggca attggagccg ggaatttata tcctccggca ggcgccagac
      301 cggaatgatg acggaatccc ggacgaagcc ggaacggaac aaaaaattga actgaaagaa
      361 cgggtcaatg ccatcggtct taccattcca tcaaaaaaag aaacgctgct gacgatcact
      421 cagcttaaat cctatggccc ggcggatgcc gatctggctg acgctgcgct cagcgcggac
      481 gatatccgct ttccggatac ggcaatggcg gcgggacagt tatcccgcat acaatgtaag
      541 attcataata tcggcagcag ggccgcagaa aatctaataa tagaatgttc ggtcgaccat
      601 aaaaaaacag ggcagcggac aataccggtt ctggaagcac ccaacgattt aaaaccgcgc
      661 tggaccacgg ttgactttat gtggaaggct ttgccgggag aacaccggat cgggataaaa
      721 attatttcgg cgcaacggga aataacgaaa cagaataatg aggcggaatc tgttgtccgg
      781 gtccggtaag taagggaaaa cgaaagccaa aatagtttgg tatttttgaa atgcattaaa
      841 aatcaggtta tggatattgt cgatgcggag caaaaggatt ttttccggga gaacgggtat
      901 ctcgttatag aaaatatttt aacagaggag gagatcctgt tttaccggaa tatttacgaa
      961 gaattcctgt ccaaccggat agatgcatcc cgttaccgct ccgatctggg cgcccatgcc
     1021 ggggaagatg caaaggcgcc gcgcggtgaa cgcatcacgc agatcatgct ggtgagccgg
     1081 ctgatgccat ccattcttga tctgcccctg caccataaaa cggcggccat tgccaaacaa
     1141 ttgctcggcg aggatatgga actggatttc gatatgctga tcgacaagcc gccgaattcg
     1201 gctaccgcga cgccctggca ccaggactgc gcctactgga tcagcatgcc cgatacccgg
     1261 gctgccagtt gctggaccgc tctcgacgat gccacgaaag aaaacggcgg aatgtggtac
     1321 gtggccgggt cccatcacct gccggtccgt tcccaccggc ccgcggggaa gggcgggggc
     1381 gcgctcgaat gcgatgcgac ggaagcggag gcaacggctg tcgaaataaa agcggggtcc
     1441 tgcatctggc atcatggcgg aacgattcat tacagccggg gaaacacgac cggccttcgc
     1501 cggagagcat tcatcacgaa tttcaggccc gctgaaatga tcgcctacga gcggctccgg
     1561 ggctttgatc ataccggcga gcgggcggtg agggatgacc gcgctgccgt tgaaaacggc
     1621 ggtcctcgcg ggccttttcc ggaaggcgca ccgcaataat tttgtcttat ttacgggtac
     1681 gtacccgtaa ataagaaaaa agttttacat tcgggctgtc gacaggggtg aaggcgccga
     1741 ccgcctggaa aatgtgctca tatgaaattt ggattaggga ctccggccat gatgattgta
     1801 ttcctgctcc ccggggtggc gggaaaggcc accgcggcgt catcttcggg aattcacagc
     1861 ctcgctgcgg ttcccgtgtt gcccttgccg cagcacatcg aaacatatcg gactgaatcc
     1921 ggaccgccgg tgacggcagg ctccctgctg ggaaaaggat tgacgcccgc tgcaaaagaa
     1981 cgcctgctct tccactggtc aggatttttg aaagaattcg cggcgggcgg cccggcgcag
     2041 gagaaaccgg atgtcgtgga gatcggaata ttggggaaag atggatcatt tgataaagcg
     2101 gttgcgcaat gggcatcgcc ctggtcgggc cggatcggaa aggaaggtta cctgctcatt
     2161 ctccggaaca ggaaacggtt catcgctgcg cacaccgaga ccggattatt ttacggcatt
     2221 cagtcgctga aacaattgat cagggcaggg tggaaaggag atctgttcat tgccgactgg
     2281 ccttcttttg agacccgcgt gatctatgat gatatcagcc gggggcccat ttccaccgtg
     2341 gcctatataa aaaaacagat cgaacggatg gccgagataa agatcaatta tctttccttt
     2401 tatattgagc atatcgttca gccccgttca catcctgatt ttgcgccacc cgacggaaag
     2461 ctgaccaccg cagatataac ggaattatcg gcttatgccg caaaattcca catgcagctc
     2521 atcggcagct tccagtcatt tggacatttt gaaaaaatat tatctctgcc gcaatatgcc
     2581 tccatgggag ccacatccac actcatctcc ccaaccgatc cgcgggcaaa acaatttttg
     2641 gaagaggtga tcggggaatt gtgcggagct ttcaatgcgc cctggtttaa tgtcaattgc
     2701 gatgaaacct ttgatctggg gcagggcagc accaaacccc tggtggacag cctgggagca
     2761 gttgcggtct atgccagtca tctccgtttt ttatacgata tcgtgaaacg gcatggaaag
     2821 aagatgatga tgtggggcga ttttgccgta aagcatcccg aagtgctgga caggctgccc
     2881 agggatatcg tatatcttac ctgggaatac ggggaccagt catcctttga ccactggata
     2941 aagccatttg ccaaccggca tctcaactat atgatttgtc ccggtattct caacagctac
     3001 cggatgtttc ccgacatgca gctggcccgc gcaaatatta ccggatttac aaaggccggc
     3061 cacgggcagg cggcgaaagg ggtcattacc accgtatggg atgatggtgc ggtgtatctt
     3121 ttttcgtccg actggtatgg ggtatatgtg gcggcggaaa ggagctggac cgtcgattca
     3181 acggctgtcc cttcattcaa cacccggtac gaacaaacgg cttacggcac ccgtaacggg
     3241 gattatgtaa aggcgctttt taccctgatg gagttgaaga agctgccgct gacctatgat
     3301 ctcagtgacc gcgtgtggca gcaaaagatt cttccggaca gcgggaaaaa actgatcgtc
     3361 aataataccg gtgtggtaga agccggccgg atcgtcggcg aagccgaaca ctatatccgt
     3421 tcgtcggatc caaaaagaaa tcaaacggac ataagcacac tggccaaccg gatcgcattg
     3481 tataaactga tcctggatac ccggctgaaa acggtggcca tcgccgctga ctatacgagc
     3541 gccgccgcgg gaacgccgca gcaggccgga gtattattca cccgcgatat aaaggaaacc
     3601 ggggatctga tacaagcata taccgcttca agggaaacct tcagacaggc ctggctgaag
     3661 gagaatcagc attactggct ggacagcgcg ctgacggctt atgataaaaa gataaaccgc
     3721 ctgacccggc taagggaaac gctgaagcag
//
LOCUS       DASYCU010000460         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_824031, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000460 DASYCU010000000
VERSION     DASYCU010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824031"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="VGM24_09260"
     CDS             <1..1658
                     /locus_tag="VGM24_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4155606.1"
                     /translation="LSVNKGGKSGGPGYVNGLYRTINEFSSILNHNLNINYVNNLTDK
                     ISLSVDAGLDSRKDEYNQSGLRSTQQIVFGLFDHSNFITHDILSEGGNDLDYKIQEIR
                     IGAYAQAVLGYADYLYLTLNGRNDWVSTLEAKNRHLFYPGISASFIPTSFWGALKNSS
                     AINYLKLRLGYSTSAHFPSPYNTRPSLDIVTNAFVDRNGNVINTGAIPDNVPNPDLKP
                     ELLRETEAGIEGKFLNNRLSLDLTGYSRTATDQILSRQLDASTGYTTTTINAGSIDNK
                     GIELSLGYVIVSSSSWKWELTGLFTLNRNRVHDMPSDIKQIQTGGYTGQVGTYAVNGE
                     PLGVIKGTYIQKDPKSGLYIVDNNGYYLQSADNAVIGDPNPKYKLTGISNLSYKDFSF
                     HMQWDYTQGGQIYSNTVRTMLSRGVTRDTDFDRSLPYVLPHTVKQDGTPNDIQQSVDN
                     IYFNSFGFGPDQTGIYDATLIRLRELSISYMIPANRLKNTPFGSASITVTGNNIWYLA
                     PHFPKYTHEDPEVNSLGSGPDHGLDLFAGPSSRRIGASIILSF"
     gene            1674..3395
                     /locus_tag="VGM24_09265"
     CDS             1674..3395
                     /locus_tag="VGM24_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HEY4155607.1"
                     /translation="MKLKCIIWFFLMLILTASCKRVNDNLSGLLNDPSKPSLEAADVD
                     LYLNNIQINFANFYSSYDNVGNILGVSDFGGQVTRMEVMYFQNTYLNAYFPGNFDQLW
                     TDAYVGVFKSANIMIPLAEEKGEFVHAGIAKVIKAYTMITLVDFFGDIPYSEANLGVA
                     NPNPHADHGAVIYDSALALLDSAIIDLGLPSSTAPSNDLFYGGDPAKWTTLAKTLKLK
                     AYVQIRLVDQTVKDKINDLIDEGDLVYTSPEDFAFNFSTHSQSPDSRHPKYLWNYGTD
                     NSAQDYIGNYFMWALDEEKGIVDPRIRYYIYRQIDDVFNDPRLPDQTTAQFDIPCLFR
                     PIPANYPPGTPYCIVGSGYLGRDHGNNEGIPADNGLRSTWGLYPAGGAFDANQNSAVG
                     QSTGFVTGAQGAGIEPIWLSSFTYFIEAEAALILGTPGDPRDLLEKGVNASFDKVSHF
                     ASAIGYNLPTSDTAFLITPTKQQKYVDKVLSLYQAAGSSEDKLNVIMKEYYLAAWGNG
                     VEPYNNYRRTGKPDNMQVAVNPAPGDFIRTFYYPAVYVDFNKNTTQKTLTTVKTFWDT
                     NPDNFVH"
     gene            3406..>3926
                     /locus_tag="VGM24_09270"
     CDS             3406..>3926
                     /locus_tag="VGM24_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155608.1"
                     /translation="MKKVIGVLFSLGLVLFMGNACKKQIINGDRSVLVTGAYITLNSA
                     VNTNLDFSNPDAKVAITIGSVGSEVASVNIYLATGANALDTNQWKLLKNVPYSKDVEL
                     DVTTSEIAAALDPDVISPGNQYVFQNEIILKDGRKFSVSNTPANFTSQPGYNMAFTWT
                     ATAVCAFDLASSVG"
BASE COUNT         1080 a          938 c          924 g          984 t
ORIGIN      
        1 ccttatccgt taacaaaggt ggtaaaagcg gtgggccggg ttatgtaaac ggcctttacc
       61 gcaccatcaa tgaattcagt tcaatcttaa accataacct gaatatcaat tatgtcaata
      121 acctgacgga taagatcagc ctgagtgtgg atgccggact ggactcccgg aaagacgaat
      181 acaatcaatc cggattaaga agcacacagc agatcgtgtt cggattgttt gatcacagca
      241 atttcataac ccatgatatt ctcagcgaag ggggcaacga cctggattat aaaattcagg
      301 agatccggat cggcgcctac gcccaggccg tgttgggtta cgcagattat ctctatctaa
      361 cccttaacgg aaggaatgac tgggtatcca cgctggaagc aaagaaccgg catttatttt
      421 atcccggcat tagcgcctcc ttcatcccga cttcattctg gggggcgtta aaaaacagct
      481 ctgcaatcaa ctacctcaaa ttgcgcctgg gctattccac cagtgcccat tttccgagtc
      541 cgtacaatac aagaccttcc ctggatattg tgaccaatgc ttttgttgac cggaacggga
      601 acgtgatcaa taccggagcc ataccagaca atgtgcccaa tcccgatctg aaacccgaac
      661 tgctccggga gaccgaagcc ggaatcgaag gtaaattcct gaacaaccgg ttaagccttg
      721 acctgaccgg ttattccaga accgccacag accagatcct ttccaggcaa ctggatgcgt
      781 ccaccggtta taccaccacc acgattaatg ccgggtccat cgacaataag ggaattgaat
      841 tgtccctggg atatgtgatc gtcagttcct cctcctggaa atgggaactg acaggccttt
      901 ttacgctgaa caggaaccgg gttcacgata tgccgtcgga tatcaaacag atccaaaccg
      961 gaggttatac cggccaggtc ggaacctatg ccgttaacgg agagcccctc ggcgttatta
     1021 aaggcactta tattcagaaa gatccgaaat ccggattgta tatcgtggat aataacggct
     1081 attacctgca atctgcagac aacgcggtga tcggtgatcc caatccgaaa tataaactaa
     1141 caggcatcag taacctgagt tataaagact tttcatttca tatgcaatgg gattacaccc
     1201 agggcggaca gatttactcc aatacggtaa ggacaatgct atcccggggc gtaacgcggg
     1261 atacggattt tgaccgctcg cttccctatg ttttacccca tacggtaaaa caggacggaa
     1321 caccgaatga catccagcag agcgtcgata atatttattt caattctttc gggttcggcc
     1381 cggatcaaac cgggatatat gacgccaccc tgatccgctt acgcgagctt tccatttcgt
     1441 atatgatccc tgcaaaccgg ttgaaaaata cgccattcgg atctgcgtcc attaccgtta
     1501 ctggcaataa tatctggtac ctggcgccac attttccgaa atacacgcac gaggatcctg
     1561 aagtcaatag cctgggttcg ggtccggacc atgggctgga cctttttgca gggccgtcct
     1621 cacgccggat aggagcgagc ataattttat cattctaaaa aagtgaaaca gatatgaaac
     1681 tgaaatgcat aatatggttt ttcctgatgc tgatcttaac cgcatcctgt aaacgggtga
     1741 atgataattt gagcggcctg ttgaatgatc cgagcaaacc ttccctggaa gcggcagacg
     1801 tcgatttata cctgaacaat attcagatca attttgccaa tttttattcc agttacgaca
     1861 atgtgggaaa tatattgggc gtatccgatt tcggagggca ggtgacccgc atggaagtga
     1921 tgtatttcca gaatacctat ctcaatgcct atttccccgg aaattttgac cagttgtgga
     1981 cggatgctta tgtgggcgtt ttcaaatcag ctaatatcat gatccccctg gcagaagaaa
     2041 aaggagaatt tgttcatgcg ggtattgcca aggttataaa agcatatact atgatcaccc
     2101 tggtggattt ttttggcgat attccctatt cggaagcgaa cctgggcgtg gccaacccaa
     2161 atccgcatgc ggaccacggg gcggttattt atgattccgc gctcgcgctg ctggactcgg
     2221 cgatcattga cctgggcctt ccttcctcga ccgctccctc caatgatttg ttttacgggg
     2281 gcgatcccgc aaaatggacg acactggcaa aaacgctcaa attaaaagct tatgtccaga
     2341 tccggctcgt ggatcagacc gtaaaggata aaataaatga cctgatcgat gaaggtgacc
     2401 tggtctatac ttcgcctgaa gattttgcgt ttaatttttc aacgcacagt cagtcgccgg
     2461 acagccgcca tccgaaatat ctctggaact acggaacaga caacagtgct caggattata
     2521 tcggaaacta tttcatgtgg gccctggatg aagaaaaggg aatcgttgat ccgaggatca
     2581 ggtactatat ttatcgccag atcgacgatg tgtttaacga tccccgttta cccgatcaga
     2641 ccactgccca gtttgacatt ccctgccttt ttcgtcccat tcccgcaaat tacccgcctg
     2701 gcacgcctta ttgtattgta ggaagtggtt acctcggcag ggaccacggc aacaatgaag
     2761 gaattcctgc ggataacgga ttgcgaagca cctggggact ttacccggcg ggcggagcat
     2821 ttgatgcgaa ccaaaattcg gctgtgggac aaagtaccgg atttgttacg ggagcgcagg
     2881 gcgccggaat cgaacccatc tggttgtcat ccttcactta ttttattgag gcggaagctg
     2941 ccttaatact gggtaccccg ggagatccgc gggatctgct cgaaaagggg gtgaatgcct
     3001 cttttgacaa ggtcagtcat tttgcttcag ccatcggata taatcttcca acatcggata
     3061 cggcttttct aatcactccg accaaacagc agaaatatgt ggacaaggtc ttatcgctgt
     3121 accaggctgc gggttcttcc gaagacaagc tgaatgttat tatgaaagaa tattatctgg
     3181 ctgcctgggg caatggagtg gaaccctaca acaactaccg gagaacagga aaacccgata
     3241 atatgcaggt cgccgttaat cccgccccgg gtgatttcat aagaaccttt tactatccgg
     3301 cggtatatgt ggattttaat aaaaatacga cccaaaaaac attgacaaca gttaaaacat
     3361 tctgggatac caacccggac aatttcgtac actaaaattc tggcaatgaa aaaagttatc
     3421 ggcgtattat ttagtttggg tttagttctg tttatgggga atgcctgtaa aaagcaaatt
     3481 attaacgggg accggagtgt actggttacg ggcgcataca ttaccctcaa ttccgctgta
     3541 aataccaacc tggatttcag caatccagac gcaaaagtgg cgatcaccat cggaagtgtg
     3601 ggatctgaag tggctagtgt aaatatatat ctggcaaccg gggcgaatgc actggatacg
     3661 aatcagtgga aattattaaa gaatgttcct tacagcaaag atgtggagct ggatgtgacc
     3721 acttcagaaa tcgcagcagc gctggatccg gatgtaataa gcccgggcaa tcaatatgtt
     3781 tttcaaaatg aaatcattct caaagacggc cggaaatttt cagtcagcaa tacacccgct
     3841 aatttcactt cgcaacccgg gtataatatg gcattcacct ggacggccac agcggtttgt
     3901 gcttttgatc tggcgtcgtc tgtcgg
//
LOCUS       DASYCU010000461         5839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1840207, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000461 DASYCU010000000
VERSION     DASYCU010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840207"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VGM24_09275"
     CDS             complement(<1..763)
                     /locus_tag="VGM24_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155609.1"
                     /translation="MFRRTLTIVLHLLLPLFMIAQASENQDSEEGGKDILDGIHRIFR
                     KSGYQEDSSFRHRHIRFGILPAAGYTLQTGFAAVVSTNMIIYKRRKQDTILPSTVIAS
                     VSYTQHKQVIFPVQSVIYFKHNRAMLISDWRFLKYPSFTYGLGMHSGPANENELDFQY
                     LKFHESILFQLTPGLYAGAGYVFDYFWNVKELHPHILPVTDFEKYGYHDRTVSSGFSF
                     HLLRDTRDNPINAYSGTFSSISVSPRFKFMGSDASW"
     gene            complement(856..2418)
                     /locus_tag="VGM24_09280"
     CDS             complement(856..2418)
                     /locus_tag="VGM24_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY4155610.1"
                     /translation="MRKATILFGTLIFISLAFIRMNDRKEGPLPGPIRIESGLISGMF
                     NERSGVTSYKGIPFAAPPIGPLRWKAPRPPEKWTGTRTCNAFGPSPMQMKPVSFLMIG
                     PEFVVPREPLSEDCLYLNVWTAAKSPDEKKPVMVWIYGGGFITGGSAAAGYSGEALAK
                     KGIVFVSFNYRLGVFGFLAHPGLSAEDPHHSSGNYALLDQLAALRWVKKNIRAFGGDP
                     DRVTIAGQSAGSASVNCLIASPLAKDLFQGAIAESGSLVLENPLLRMKKLDAAEKRGL
                     ELADKLHAKNIRELRALPTETIQKASSFYAPIVDGYVMPVSVAEIFKHSGQTHVPFLT
                     GWNADEGFLMGIKKRVDFAAEAKDFGADSALFTKYFPSATDSESLASQVALSVDKTIA
                     VPQYIWALRQNENSKAKTFLYRFTRKPPATGDKKRFGAYHTAEIGYALHNLDSIQRSW
                     EPVDRQLEEIMSSYWVNFVRTEDPNGPQLPLWPAFSGKDPQTMFFSEERSAKKLPDQE
                     ALNFLYAGYPGK"
     gene            2832..4070
                     /locus_tag="VGM24_09285"
     CDS             2832..4070
                     /locus_tag="VGM24_09285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4155611.1"
                     /translation="MLFTELGLIEPLLKAVDKEGYKTPTPIQEQAIPVVLAGRDLLGC
                     AQTGTGKTASFVLPLLQRMHQQKEAAGFKGHVIKTLVLTPTRELATQIHESIGNYGRF
                     LNLKSVVIFGGVPQSRQVQELRRGCDFVVATPGRLVDLLNQGILSLNGIEYFVLDEAD
                     RMLDMGFVVEVKRIIARLPKNRQTLFFSATMPPAILSLVQILLTKPVEISVTPAALTT
                     ELVDQSLYYVQKSEKRELLHHLLKNGGRKSVLVFTRTKHGADRVARDLNRSGFQATAL
                     HGDKTQQTRERSLQGFKDGKLQILVATDIAARGIDIDDLGLVVNFDLPNIPETYVHRI
                     GRTGRAGAFGKAISFCDFEEKIYLRDIQRLIGQTVPVVSDHPFHVKLMEPLSTAIAQP
                     SSGGRPAGGGKRYGRRFSQR"
     gene            4084..4755
                     /locus_tag="VGM24_09290"
     CDS             4084..4755
                     /locus_tag="VGM24_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HEY4155612.1"
                     /translation="MSAIPIRVILTGATGMVGEGVLHECLLSPAVEAVLLVSRKTIGI
                     RHPKLREILHSDFFDLVSIQSELSGYNACFFCLGVSSVGISKESYEKLTYDLTLGFAG
                     VLARQNPGMVFCYVSGAGTDSTEKGRVNWARVKGKTENDLQKLPFREVYNFRPGIIKP
                     TKGLKNTLPFYKWMGWLLPLIAVVFPNYLTSLADIGQAMIRVVTRGYSKKTIEVPDIR
                     ILSRD"
     gene            5096..>5839
                     /locus_tag="VGM24_09295"
     CDS             5096..>5839
                     /locus_tag="VGM24_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HEY4155613.1"
                     /translation="MMEFAPLIAEKLKIHTRQTETVLALLAEGASIPFIARYRKDKTG
                     ALDEVQIQLIQDESKSLKAFAERKMAIEKSIREQEKMTESLQAKLDHAQTLTDLEDIY
                     LPYKPKRKTKAQAAREKGLEPLALLILEQKTPDLPGAAGRFIQEEIKTADEALQGARD
                     IIAEMVNENETVRARMRRLFEETANLESRLLSGKEAEAAKFKDYFDYAELISTVPSHR
                     ILAVLRGFTEGYLRMNISPVEEEALELIGA"
BASE COUNT         1484 a         1413 c         1461 g         1481 t
ORIGIN      
        1 tccaactggc atccgatccc ataaatttaa accggggact cacggaaata ctggaaaaag
       61 ttcccgaata cgcattgatc ggattatcgc gggtatcccg aagcaggtga aaggaaaatc
      121 cggaagaaac cgttcgatcg tggtaaccgt atttttcaaa atcggtgacc ggaaggatat
      181 ggggatggag ttctttaacg ttccagaaat aatcgaaaac gtatccggca ccggcataca
      241 agccaggcgt cagttggaac aggatggatt catgaaattt aagatattga aaatccagct
      301 cgttttcatt ggcaggaccg gagtgcattc caagcccgta cgtaaaggag gggtatttta
      361 aaaaccgcca gtcggaaatc agcatggcgc ggttatgctt aaaataaatg acggactgta
      421 cgggaaatat gacctgttta tgttgtgtat aggaaacgct ggcaattacg gtggacggca
      481 ggatcgtatc ctgtttcctt cttttataga tgatcatatt ggtactgacg acagccgcaa
      541 aacccgtttg cagcgtgtat cctgccgccg gcaggatgcc aaaccggata tggcggtggc
      601 ggaatgagct gtcttcctgg taaccgcttt ttctgaagat ccggtgaatg ccgtcgagga
      661 tatctttacc accctcttcc gaatcctgat tctcggaagc ctgtgcaatc ataaacaaag
      721 gcaatagcag gtgcaggaca atcgtcagtg ttcttcgaaa cataattcgg gctcagggtt
      781 agtcgctggt tctaagataa tcaatcaaaa tcatttgcgg gtttggcaat gaacgaacaa
      841 aacggaccgt atccgttatt ttccgggata cccggcatat agaaaattca aggcttcctg
      901 atcgggcaat tttttggccg atcgttcttc gctaaaaaac atcgtttgcg gatcttttcc
      961 ggaaaaggcg ggccagaggg gcaattgcgg cccgttggga tcttccgttc ttacaaagtt
     1021 cacccaataa ctgctcatga tttcttccag ttgacggtcc accggttccc aggatctttg
     1081 aatgctgtcc agattgtgca gcgcataacc gatttctgct gtatgatatg ctccgaacct
     1141 ctttttatcg cccgttgccg ggggtttgcg ggtgaaccgg taaagaaagg tttttgcctt
     1201 gctattctcg ttttggcgga gcgcccagat atattgagga acagcaatgg ttttgtctac
     1261 gctaagcgcc acctgggaag cgagcgattc ggaatccgta gcggaaggaa aatattttgt
     1321 gaaaagagcg gagtctgcgc cgaagtcttt tgcttcggct gcgaaatcga ctctcttttt
     1381 tattcccatt aaaaatcctt catcggcatt ccagcccgtt aaaaacggaa catgggtctg
     1441 gccggaatgt ttgaatattt ctgccaccga aaccggcatc acatagccat ccacaatcgg
     1501 agcataaaaa gaggaggcct tttgtatggt ttctgttgga agcgcgcgca gctcccggat
     1561 attttttgcg tgcagtttat ctgccagttc cagtccccgt ttttccgcgg catcgagttt
     1621 tttcatccgg agcagcgggt tttccaggac cagggatccg ctttcagcaa tagcgccctg
     1681 gaacaagtcc tttgccagag gagaagcgat caaacaattc acgctggccg atccggcgga
     1741 ttgccccgca atcgttaccc ggtccgggtc tccgccaaag gcgcggatat ttttctttac
     1801 ccaccgcaag gcagcaagct gatccagcaa agcatagttt cccgaagaat ggtgcggatc
     1861 ttcggcactg agacccgggt gggcaagaaa accaaaaacg cccaaacggt aattgaagga
     1921 aacaaaaaca attccttttt tggccagggc ctctccggaa taacccgcgg ctgctgaacc
     1981 cccggtaatg aatcctcctc catagatcca taccatcacc ggtttttttt catccggtga
     2041 tttagcggcc gtccatacat tcaaataaag acaatcttcg cttaacggtt cccggggaac
     2101 aacgaactcc ggcccgatca tcaaaaaaga aaccggtttc atttgcatcg ggctcggtcc
     2161 gaaggcattg caggttctgg tccccgtcca tttttccgga ggccgcggtg ctttccagcg
     2221 taatggtccg atgggtggcg cggcaaaggg aatgccttta taggaagtga cacccgagcg
     2281 ctcgttaaac attccgctga tcagcccgct ttcaatccgg ataggtcccg gcagcggtcc
     2341 ttcctttcgg tcattcatcc ggatgaatgc aagggaaata aaaatcagcg tgccaaataa
     2401 aatcgtggct tttctcattt ggtgaattta atccattgga taccagccgg atacaagtcc
     2461 ggacttttta aggacaagat ccgcatcccg ccgccggcag ctgggaagta atcaggcagt
     2521 aatatacaat ttatgaaaaa aatcatttcc ggattttctt cttcaggggt tccccgggag
     2581 ttctttccac tggcagaaat atacaaaacc ggcattgatc gcggtctctt gcggccagcc
     2641 ggcccggata attagtcaac ttgaataaaa tgtattatct ttgtggttct gtatgttatg
     2701 actcttactg cgttttcttt tgagtgtttt atttctctcc agagttttcc cgtgacagtg
     2761 taacgtaagg caggtaaagt accctgattc agcggtagcc ttaccacatt ttttacttaa
     2821 tatttttcaa attgttattt acagaactag gccttattga gcctttgctc aaagccgtag
     2881 acaaggaagg ctataaaacg ccgaccccca ttcaggaaca agccatcccc gtggttttag
     2941 caggtaggga tctgctcgga tgcgcccaga cgggcaccgg aaaaaccgct tctttcgttt
     3001 tgccattatt acagcgcatg caccagcaga aagaagctgc cggtttcaaa ggacatgtga
     3061 tcaaaaccct cgttttaaca ccaacccgtg agctggcaac ccagattcat gaatccatcg
     3121 gaaactatgg aaggttcctg aacctgaagt ctgttgttat tttcggaggc gttccccaat
     3181 ccaggcaggt ccaggaattg cgcaggggct gtgattttgt agtagccacc cccggccgtc
     3241 tggtggatct gctcaaccag ggtattctct ccttaaacgg tatcgaatat tttgtgctgg
     3301 atgaagcgga ccggatgctg gatatgggtt tcgtggtgga agttaaacgc attattgcca
     3361 ggttgcccaa gaacaggcag accttattct tttctgccac catgcccccg gctattttat
     3421 ccctggttca gatcttgttg acaaagccgg tggagatttc tgttactccg gcggccttaa
     3481 ccaccgaatt ggtggatcag tccctttatt atgtgcagaa atcagagaag cgggagcttc
     3541 tgcatcacct gctgaaaaac ggggggcgaa agagcgtact ggtattcacc cgcaccaaac
     3601 atggtgccga ccgggtagcc agggacttaa accgttccgg ttttcaggcg acggctttgc
     3661 acggcgataa aacccagcag acccgcgaac gctcattgca gggatttaaa gacggaaaat
     3721 tacagatcct ggtcgcaacg gatattgccg cccggggaat agacattgat gacttgggcc
     3781 tggtggttaa tttcgacctg cctaatattc ccgaaaccta tgttcaccgg atcggaagga
     3841 ccggcagggc aggagctttc ggaaaggcca tttctttctg tgattttgaa gaaaaaattt
     3901 atctccggga tatccagcgg ctgatcggcc agaccgttcc ggtcgtcagt gatcatccct
     3961 ttcatgttaa actgatggag ccattgtcca cggcgattgc ccagccttca tccggaggac
     4021 gtccggccgg cggcggcaag cgttatggaa gaagattctc gcaacgttaa tgcctgaaag
     4081 tttatgtcgg caattcccat ccgggttata ttgactggcg ctaccggtat ggtgggggaa
     4141 ggcgttcttc atgaatgcct gctttccccc gcagtggaag cggtattgct ggtcagcagg
     4201 aaaaccattg gcatccggca ccccaaactc agagagatcc ttcacagtga ttttttcgac
     4261 ctggtttcca ttcagtcaga acttagcggt tacaacgcct gttttttttg cctgggggtc
     4321 tcttccgtag gcatcagcaa agaaagctat gagaaactca cttacgatct caccctgggc
     4381 ttcgcgggcg ttctggcaag gcagaatccg ggaatggtat tttgttatgt ttccggagcc
     4441 ggaaccgatt caaccgaaaa aggaagggtg aactgggccc gggttaaagg aaaaacggaa
     4501 aacgatttgc agaaacttcc cttccgggag gtttataatt ttcgcccggg aataatcaaa
     4561 ccgacaaaag ggttgaaaaa cacgcttcct ttctataaat ggatgggctg gcttcttccc
     4621 ctgatcgccg tcgtttttcc aaactatctt accagcctgg cagatatcgg acaggccatg
     4681 atccgggtgg tcacccgggg gtactcaaaa aaaacgattg aagttccgga tatccgcatt
     4741 ctttcgcggg attaactgga tttggacctg cgccggaacc ggttaatcgg atcctttccc
     4801 gccaacggcg gcgaataccc cttttaaacc ggtaaactgg aattttttat ccggatactg
     4861 gataatccga aggctgatat tttttccctc ctgctcctaa cccggagcag taaatattaa
     4921 tgtatttttt acacttatta ttgcaaggct tcctttcatc gcttaccttt gatccgcgaa
     4981 cccaaaaaat atttatgttt ttggtaagca atgtttagaa gcccccctgt tcccaggagg
     5041 gcttttttta ttaacaccct tcccggtatc tgcccagccg ttagctttgc atcctatgat
     5101 ggaatttgcg ccgctgatcg ctgaaaaatt aaagatccat acccggcaga cggaaaccgt
     5161 actggcttta cttgctgaag gagcgagtat tccatttatt gcccgttacc ggaaagacaa
     5221 aacgggggca ctggatgaag tgcagatcca gctgatccag gatgagtcga aatcgctgaa
     5281 ggcatttgcc gaaaggaaaa tggcgattga aaaatccatc cgggagcagg agaaaatgac
     5341 cgaatccctt caggcaaaac tggatcatgc acaaaccctg accgatctgg aagatattta
     5401 tctgccctat aaacccaaac ggaaaacaaa ggcgcaggcg gcccgcgaaa aaggactcga
     5461 gccattggct ttgctgatcc tggagcaaaa aacccccgat cttcccggtg cagccgggcg
     5521 ctttattcaa gaggaaataa aaacagcaga cgaggcgctg cagggcgccc gggatattat
     5581 tgctgaaatg gtcaatgaaa atgaaaccgt gcgcgcccgg atgcggcggc tttttgaaga
     5641 gacggcaaat ctcgaaagca ggctactttc gggaaaggaa gcggaggcgg caaaatttaa
     5701 agactatttt gattatgccg aactgatctc tacggtcccg tcccaccgga tactggctgt
     5761 gctaagggga tttacggaag ggtatctccg gatgaacatt tctcctgtgg aagaagaagc
     5821 attggagctg atcggggca
//
LOCUS       DASYCU010000462         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1840266, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000462 DASYCU010000000
VERSION     DASYCU010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840266"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..279)
                     /gene="rpsS"
                     /locus_tag="VGM24_09300"
     CDS             complement(4..279)
                     /gene="rpsS"
                     /locus_tag="VGM24_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY4155614.1"
                     /translation="MGRSIKKGPYVDANLETKVLGINDGKAKKSVIKTWSRRSTITPD
                     FVGHTFAVHNGNKFIPVYVTEFMVGHKLGEFAPTRNFKGHSSKKIAE"
     gene            complement(361..1200)
                     /gene="rplB"
                     /locus_tag="VGM24_09305"
     CDS             complement(361..1200)
                     /gene="rplB"
                     /locus_tag="VGM24_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY4155615.1"
                     /translation="MALKKFKPVTAGTRWRIGNAYAEVTSDNPEKSLLEKVKSTAGRN
                     SQGRRAMRYKGGGHKKMYRQVDFKRDKKSIPGVVASIEYDPYRTAFIALLHFSDGEKR
                     YIIAPQGLQVGTTVLSGDAVAPEVGNALQMRNIPLGTNVHNIEMQPGQGGKLVRSAGA
                     SAQLTNKEDKYAVLKMPSGELRKILINCYATVGVVSNSDHSLQSMGKAGRNRWRGIRP
                     RVRGVAMNPVDHPMGGGEGKASGGQPRSRNSQYSRGLKTRTRGKGSDKLIIQTKKGKK
                     ISK"
     gene            complement(1272..1571)
                     /gene="rplW"
                     /locus_tag="VGM24_09310"
     CDS             complement(1272..1571)
                     /gene="rplW"
                     /locus_tag="VGM24_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY4155616.1"
                     /translation="MKLSEVLIRPILTEKANAQQEKLRRYAFKVNRKANKLEIKKAVE
                     DFYGITVLDVNTAVVPSKARSRSTKAGLITGRKPGYKKALITLAEGDTIDLYSNI"
     gene            complement(1602..2234)
                     /gene="rplD"
                     /locus_tag="VGM24_09315"
     CDS             complement(1602..2234)
                     /gene="rplD"
                     /locus_tag="VGM24_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY4155617.1"
                     /translation="MQVDIVNIKGDKTGRTLDLPAEIFEAEPNDHVIYLAVKQYLAAQ
                     RQGTHKVKTRMEVKGSSKKLHKQKGTGGSRKGNIRNPLYKGGGTIFGPKPRSYDFKLN
                     RKVKDLAKISALSHKAKANSIVVVEDMKLETPKTKELLNLIGGLKLDGKKTLFVLPEY
                     NDNLHLSLRNVPYLESSLLADVNTYDIVNAQILVLTESAARMFMEEEVEA"
     gene            complement(2252..2869)
                     /gene="rplC"
                     /locus_tag="VGM24_09320"
     CDS             complement(2252..2869)
                     /gene="rplC"
                     /locus_tag="VGM24_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY4155618.1"
                     /translation="MKGLIGKKIGMTSVFTPDGKQVACTIIEAGPCIVTQVKTAESDG
                     YSSLQLAFGDKKEKHTLASEKNHFAKSNTAAKKFIKEFRNFSLEKNLGDALTVDIFTE
                     GEKVDVVGTSKGKGFQGVVKRHGFHGVGGQSHGQHDRSRAPGSLGNSSDASRVMKGVR
                     MAGRMGGDRVKLKGLNVVKIFPDKNYLLISGSVPGHNGSIVYIQK"
     gene            complement(3180..3485)
                     /gene="rpsJ"
                     /locus_tag="VGM24_09325"
     CDS             complement(3180..3485)
                     /gene="rpsJ"
                     /locus_tag="VGM24_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY4155619.1"
                     /translation="MSQRIRIKLQSYDHNLVDKSAEKIVKTVRSTGAVVTGPIPLPTR
                     KKVFTVLRSPHVNKKAREQFQLHTHKRLLDIYTSTSRTVDALSKLDLPSGVDVEIKA"
BASE COUNT          779 a         1000 c          822 g         1125 t
ORIGIN      
        1 tccctattca gcaatctttt tgctggaatg tcctttgaaa ttgcgggtag gtgcaaattc
       61 gcccagcttg tgacccacca tgaactccgt tacatacacc ggtatgaact tgttgccatt
      121 atggacggca aaagtatgtc ctacaaaatc cggtgtaatg gtggaacggc ggctccaggt
      181 cttaatgacg cttttttttg ccttcccatc attgatgcca agcactttgg tttccagatt
      241 cgcatctacg taaggacctt tttttattga acgacccata attaatgagt taatttgaaa
      301 tgtgaaattt attttccggg gtcaatatga atttcctttt cacagggacc cgccgattac
      361 ttacttgctt attttctttc cttttttcgt ctggatgatc agtttatcgc tgcctttccc
      421 gcgggtcctg gttttaagtc ctctggaata ctggctgttc ctggaacggg gctggcctcc
      481 ggatgctttt ccttctccgc cacccatcgg atgatcaacc gggttcatgg ccacaccacg
      541 cactctcgga cgaattcccc tccagcggtt acgcccggct ttacccatgc tctgcaggct
      601 gtgatcgctg ttggaaacca cccctacggt ggcataacag ttgatcagga ttttccttag
      661 ttcaccggac ggcattttta gcaccgcata tttatcttcc ttgttggtca gctgtgcgga
      721 tgctccggca ctacgaacca gctttccccc ctgacccggc tgcatctcaa tgttatgcac
      781 attggtcccc agcggaatat tcctcatctg cagcgcattt cccacttccg gggcaaccgc
      841 atcaccggac agaacggtcg ttccgacctg caaaccctgg ggcgcgataa tatatctttt
      901 ttccccgtcg ctgaagtgca gcagggcgat gaatgctgtt ctgtacggat catattcaat
      961 ggaagccact acccccggga tggatttctt gtccctttta aaatccacct gccggtacat
     1021 ctttttatgt ccgcctccct tgtaacgcat tgcccttctg ccctgggaat tacggccggc
     1081 agtcgacttc actttctcca gcaggctctt ttccggatta tccgaagtaa cctctgcata
     1141 cgcattgccg atcctccacc tggtgccggc tgttaccggt ttaaattttt ttagtgccat
     1201 attttcttct aaagaatatt tataatgaga gcttcaactt tcgcggcagc tgaatgccat
     1261 tctttaatta cttaaatatt cgaatacaaa tcaatggtat ccccttccgc cagcgtgatc
     1321 agggctttct tgtaacccgg cttacgccct gtgatcaatc cggctttggt agacctcgag
     1381 cgggccttgc tgggaaccac cgccgtattt acatccagca ccgtgatccc gtaaaaatct
     1441 tccacggcct tcttgatttc cagtttattg gcctttctgt ttaccttgaa tgcataccgg
     1501 cgcagctttt cctgctgggc atttgctttc tccgtaagaa tcgggcggat cagaacctca
     1561 cttaatttca tcttgataaa tttgaaaaat tgttacacat gctatgcttc cacttcttcc
     1621 tccataaaca tcctggccgc cgattccgtc agcaccagga tctgcgcatt tacgatatcg
     1681 taggtgttca catcggccag caggctgctt tccaggtaag gaacattcct taaactgaga
     1741 tgcaggttat cattgtattc cggcagaacg aacagggtct ttttgccgtc cagtttcaat
     1801 ccgccgatca ggttcagcag ctcttttgtt ttcggtgttt ccagcttcat atcttccacc
     1861 accacaatgg aattcgcctt tgccttatgg gacagggcag agatcttggc caggtccttc
     1921 accttccggt tcagcttgaa atcgtaactc ctgggcttgg gtccgaaaat agtaccccct
     1981 cctttataca gcgggttccg gatatttcct ttacgggaac caccggttcc tttttgtttg
     2041 tgcaatttct tggaagaccc ttttacttcc atccgcgtct tcaccttatg cgttccctgg
     2101 cgctgggctg cgagatactg ttttacggcc aggtaaatca cgtgatcatt cggctccgct
     2161 tcaaagatct ccgcaggcag atccagtgta cggcccgttt tatctccttt tatatttact
     2221 atatctactt gcatttgaat gggattaata gttacttctg aatgtaaaca atggagccgt
     2281 tgtgacccgg aacagatccg gagatcaaca ggtaattttt atccgggaat atttttacca
     2341 cgttcagccc tttcagcttc acccggtccc cgcccatgcg gcctgccatc cggacacctt
     2401 tcataacgcg ggatgcgtca gaagagttac ccagtgatcc gggcgcccgg ctgcggtcat
     2461 gctggccatg cgactgaccg ccgaccccat gaaatccatg gcgttttaca accccctgga
     2521 atcctttacc tttcgaggtg cccaccacgt ccactttttc tccttccgtg aaaatatcta
     2581 cggtaagagc gtcccctaaa ttcttttcca gggaaaaatt gcggaattct tttatgaatt
     2641 tctttgcggc agtatttgac ttggcgaagt gattcttttc ggaagcgagc gtatgctttt
     2701 cttttttgtc gccaaaggcg agctgaagtg aactataacc gtctgactcc gcggttttga
     2761 cctgtgttac aatacaggga cccgcttcaa tgatggtgca ggctacctgc ttgccatccg
     2821 gggtgaacac gctggtcatt ccaatctttt ttccaattaa ccctttcatc ttttattggt
     2881 ttgtgcctgg agtctaagga caatcagcgc tttcgtttca gccggctggc tgatactgat
     2941 ttcaatcccc tgtttgcaac cgaccttttc tgtttgtcac cgaagtttca gcccgggcgc
     3001 ctttcgacgc tcccgcttcc ccttcgtcat ccttccggat ggccgccttc gccagggcgt
     3061 cagcagctga tagaagtacc ggtagtaaac aaaccccgaa cggcttgttc atattttgta
     3121 ttcaaagatc cagcgctgct ttcaccgctc cggtttggca gatggaatat taacctttat
     3181 caggccttga tctccacatc cactccggat ggaagatcaa gcttggacag ggcatctacc
     3241 gttctggaag tagatgtgta aatgtccaat agtctcttgt gggtatgcaa ctggaattgt
     3301 tcccgcgctt tcttgttcac atggggcgaa cgaagtacgg tgaatacctt tttccgggtc
     3361 ggcaggggaa tgggtcccgt taccacagct cccgtgctgc gtactgtttt tacgatcttt
     3421 tcagcggatt tatccacgag attatgatcg taagactgca atttgattct gattcgctga
     3481 gacataggcc aatctccgtt ttttgaacgg gaggcaaagg tagcgataac cgcagatacg
     3541 ggcaaagaat tatcctgttt ttttggagaa ttatttgggg ccgcccgggc aatgttcatt
     3601 cagatatctg gtatacaggg accccaggtg ctgccgggtg cccggtcctt cgctgatgtc
     3661 gtgcgtcctg ttgggataag ccatatactg aaaggtctta ttgtacttaa tcaactcatt
     3721 caccag
//
LOCUS       DASYCU010000463         7535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_534853, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000463 DASYCU010000000
VERSION     DASYCU010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534853"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..633
                     /locus_tag="VGM24_09330"
     CDS             40..633
                     /locus_tag="VGM24_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY4155620.1"
                     /translation="MIKTGNPVISIYTEMTPNPETMKFVANKLLYPGKSIDFPDVESA
                     RPSPLAIELFGFPFIKSVFIASNFVTLTKTSDTDWADVIPSIRQFLKDYLEDAKPVII
                     EEEITVAHTDGNAIGADDDDVVKRIKELLESYVKPAVEMDGGAIQFKSYNDGTVNLML
                     HGSCSGCPSSMITLKAGIEGMMKRMIPEVREVVAEAD"
     gene            714..1322
                     /gene="pnuC"
                     /locus_tag="VGM24_09335"
     CDS             714..1322
                     /gene="pnuC"
                     /locus_tag="VGM24_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEY4155621.1"
                     /translation="MQQIVHQFINDIYQSTWLEFIAVIFGIGSVIYSRRENILVYPVG
                     MVSTGIFIYLYILHGLYADASVNFYYTVMSIIGWYMWSKKGSGKPLLKMTASTATDWR
                     NALIFFFSCWLVLYLVLKHFTDSTVPMADSFTSGAAFTGMWLMNKKKLENWTWWIITD
                     LASVPLNFYKHLVFASFQYLVFLVLAIMGYITWSKKIHHAGA"
     gene            1309..1842
                     /locus_tag="VGM24_09340"
     CDS             1309..1842
                     /locus_tag="VGM24_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4155622.1"
                     /translation="MPAPKKVVVIGPESTGKSTLCSALAAHYQTSWVPEFAREYLLGL
                     GRPYRYDDLLLIAEGQLRTEDEMTKQMTGKILFIDTDMYVMKVWCEFVFGKCFPFILD
                     QIAERSYDLYLLCNTDLPWVKDELREYPDPVNRNKLFRIYKDLLINQHCPWVEIRGLN
                     EERIQQAIEAVGKWLLQ"
     gene            complement(1831..2532)
                     /gene="mtgA"
                     /locus_tag="VGM24_09345"
     CDS             complement(1831..2532)
                     /gene="mtgA"
                     /locus_tag="VGM24_09345"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY4155623.1"
                     /translation="MAIKTKGLVPRTWRWIKRILIFLFFFHFFYLLILKWVNPPVTLT
                     QLSSLFHGKGLKRDYVGIKDISPYARLAVIASEDQLFPDHDGFDFKSIEKAMKHNQKS
                     KSLHGASTISQQVAKNVFLWQGRSWLRKALEAYFTFMIEKVWGKQRILQMYLNVSEMG
                     KGVFGIEAAAKYYFNKHAKSLTRTESAMIAGCLPNPVLYTIKPMSRHVASRYPWILNQ
                     MNHLQDDPDVIKLLQ"
     gene            complement(2545..3075)
                     /locus_tag="VGM24_09350"
     CDS             complement(2545..3075)
                     /locus_tag="VGM24_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155624.1"
                     /translation="MQKIFAVLLFLLPLAGFSQTDVLILQKNGRNIKTYAPGERIIFE
                     TIYDQWFDGMLTELRNDSVFVNHIPFHYNEIRAMRTERGKLNYSQDGTLLIIAGVGVI
                     VLNTVNGLYRKDKAKDWFSTTGWITAGVLIAGGILLRKSRIKTYPLGKKYGLHYLDLH
                     LDNPGSRIAHPPGNPH"
     gene            complement(3108..3752)
                     /locus_tag="VGM24_09355"
     CDS             complement(3108..3752)
                     /locus_tag="VGM24_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY4155625.1"
                     /translation="MIEAIWKGLALGIVLALSVGPIIFTIIKQSITSGHKGGFSFVAG
                     VWLSDVILVVLSNVFTEWVTRLLEYKKLIGYGGSSFLIALGVYFFFFKKVHLKATTGS
                     MHLDDFSVHDMLKTFASGFLINTLNPGVLAFWLGNATVLALTHTLKQRIVIFSTCLLI
                     NMAADVGKVMMAGKLRKRLTVHNLNIINKISGAILIAFGIALLYGVIFLEHRIR"
     gene            complement(3832..5370)
                     /locus_tag="VGM24_09360"
     CDS             complement(3832..5370)
                     /locus_tag="VGM24_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEY4155626.1"
                     /translation="MALIDVQLPNSLARALRLPKKSARRQQLKVLKKLLKKARFTEFG
                     QQYHFDRILLGKRILKNFQENVPVYDYSKIYKEWWYKTLEGKPDICWPGKIKYYALSS
                     GTSEAASKYIPVTNDLLRGNRVVMVRQLLSLRNYQDIPVKSIGKGWLMIGGSTDLQKG
                     PGYYEGDLSGISAKKVPFWFSPFYKPGKRIAKTKDWNRKLEEIVEQAPNWDIGFIVGV
                     PAWIQMCMEKIIEKYQLKNIHEIWPNLGFFVHGGVSFEPYKRGFEKLLGKPIIYIETY
                     LASEGFIAYQDRQDERGMRLVTNQHLFFEFVPFDSSNFDQDGNIVKDPAALQINEVEE
                     GKDYALLISTCAGAWRYLIGDTIRFTNKEKSEIIITGRTKHYLSLVGEHLSVDNMNQA
                     IQLVSQEFNISIPEFTVTGIPHGLFFAHQWYIASETGLDPDLIRNRIDENLKRLNDDY
                     AVERKSALKDIRVKVLTPEVFMEFMRASGKEGGQHKFPRVLKGKMLKDWLNFLDKISE
                     KIQY"
     gene            complement(5444..6967)
                     /locus_tag="VGM24_09365"
     CDS             complement(5444..6967)
                     /locus_tag="VGM24_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEY4155627.1"
                     /translation="MKRFFFPVIPVLFSLIVSGQPKYEFRAVWIATVDNIDWPSRGNY
                     NTELQKEEFIRQLDMHRRNGMNAVVVQVRPACDAFFPSSLEPWSEWLTGRQGKAPSPY
                     YDPLEFMIRETHKRGMEFHAWCNPYRAVFRIGRSSIAANHISRVHPDWVITYGDTKYL
                     DPGNKEVQAFVVSVVRDMLRRYDIDGIHFDDYFYPYRIAGKEFPDNASYLKYGNGMNK
                     EDWRRSNVDSVISQLSRAIHEEKKTCKFGISPFGVWRNLSQDTAGSDTHAGQTNYDDL
                     YANILLWLKNGWIDYVVPQLYWEFEYKNAPFGVLLNWWSKHSYGRPCYIGLGYYRAGS
                     SIYWRDKNQLPRQLTAIRNTENIQGEVYFSSTSFYRNPYGWNDSLQQHYYNYPALIAP
                     LPWLDSTKPAAPVLRINDTRDSLVFHFEKGSPSDTLRGFAIYKTTGRFPTDSSGRFNI
                     DSERVFLFIPDNEDPKLLFKVADLQGKRNERYLVTAISKTNNESEPVPLYPVTVSNP"
     gene            complement(7012..7473)
                     /locus_tag="VGM24_09370"
     CDS             complement(7012..7473)
                     /locus_tag="VGM24_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY4155628.1"
                     /translation="MTLEDDINQHVFRNEYQKAVLNLIYTFNWMNERLNKSFDPFEVT
                     QQQFNILRILRGAGTPLSTLQIRQRMLDKMSDTSRIVDRLIKKGLVKKSVCHEDRRLV
                     DVLLTDKGKKLLQQMDTLNTEFDAVLQQLSEEDAGKLNELLDKIRSAEKEG"
BASE COUNT         1895 a         1872 c         1744 g         2024 t
ORIGIN      
        1 cccacgaaaa tgttattttt gcattataat aagtgagcta tgataaaaac tggcaacccg
       61 gtcatcagca tttatactga gatgaccccc aaccccgaga ccatgaagtt tgtggccaac
      121 aaactcctgt acccggggaa aagcattgat ttcccggatg ttgagtctgc caggccctcc
      181 ccgctggcca tcgaactgtt tgggttcccc tttattaaaa gcgtctttat cgccagcaat
      241 tttgttaccc tcaccaaaac cagcgatacc gactgggctg acgtgattcc gtccattcgc
      301 cagttcctga aagattatct cgaagatgca aagccggtga tcatcgaaga agagatcacg
      361 gtagcccata cggacggaaa tgccatagga gcggatgacg atgacgtggt aaaacggatc
      421 aaagaattac tggagagtta tgtaaagccc gccgtcgaaa tggacggagg cgctatccag
      481 tttaaaagtt ataacgatgg aaccgtcaac ctcatgttac atggttcctg ctcaggctgt
      541 ccttcttcca tgatcacgct gaaagccggt attgaaggca tgatgaaacg catgattccg
      601 gaagtaagag aagtggtggc tgaggccgac taaatttcaa cccgatgatt ttcttcccgc
      661 ctccggcggg atttttttta acttcccggc tttcaaccct tcaatttttc cggttgcagc
      721 aaatcgtcca tcagtttatc aacgacattt atcagtccac ctggcttgaa ttcatcgcgg
      781 tgatcttcgg tattggaagc gtgatctatt cccgcaggga aaacatcctg gtttacccgg
      841 tgggcatggt gagcaccggc atttttattt atctctacat cctccatggt ctctatgcgg
      901 atgcgtccgt aaacttttat tataccgtca tgagcattat cggatggtat atgtggtcga
      961 agaaagggtc cggcaaaccc ctgttaaaga tgacggcttc caccgccaca gactggagaa
     1021 atgccctgat cttttttttc agctgctggc tggtcctcta cctggtcctg aaacatttta
     1081 ccgacagtac cgtgcccatg gcggatagtt ttacttcggg agctgcattc accgggatgt
     1141 ggcttatgaa taaaaagaaa ctcgagaact ggacctggtg gatcattacc gacctggctt
     1201 ccgtcccgct caatttttac aagcacctgg ttttcgcgag ctttcaatac ctcgttttcc
     1261 tggtcctcgc cataatgggt tatattacct ggagcaaaaa gatccaccat gccggcgcct
     1321 aagaaagtag tggtcatagg tcctgaatcc accggtaaga gcacgctctg ttccgcgctg
     1381 gccgctcact atcaaaccag ctgggtgcct gaatttgcgc gggaatatct actcggcctg
     1441 ggcaggcctt accggtacga tgacctgctt cttattgcgg aaggccagct ccggacggaa
     1501 gatgaaatga cgaaacaaat gacgggaaag atcctgttca tagataccga tatgtacgtg
     1561 atgaaagtat ggtgtgaatt tgtttttgga aaatgcttcc cgtttatcct ggatcagatc
     1621 gcggaaagaa gttacgatct ctacctgctg tgcaataccg atctgccctg ggtgaaggat
     1681 gaattaaggg aatatcctga tcccgtgaac cgaaacaaac tctttcgaat ctataaggac
     1741 cttctgatca accagcattg tccctgggtg gagatcaggg ggctcaatga agaaagaatt
     1801 cagcaggcca tcgaagccgt gggcaaatgg ctattgcaat aatttaatga catccgggtc
     1861 atcctggagg tgattcatct gattcagtat ccagggatac cgggaagcca cgtgccggga
     1921 catgggtttg atcgtgtaca gcaccgggtt gggaaggcat cccgcgatca tcgccgattc
     1981 cgtgcgggtc agcgatttgg cgtgcttgtt aaaataatat tttgccgcgg cttcaattcc
     2041 aaaaacgcct ttgcccattt cgctcacgtt gagatacatt tgaaggatcc gttgtttgcc
     2101 ccagactttt tcaatcataa acgtaaaata ggcttcgagc gccttgcgca gccaggatct
     2161 tccctgccat aaaaagacat tcttggccac ctgctgactg atggtactgg cgccatgaag
     2221 cgacttactc ttctgattat gcttcatggc tttttcgatc gatttaaaat caaatccgtc
     2281 atgatcggga aatagctgat cttccgaagc gatcacggcc agcctggcgt aaggtgagat
     2341 atcttttatt ccgacataat cccgcttcaa cccttttcca tgaaacaggc tgctcagctg
     2401 ggtgagcgta accggcgggt ttacccactt taagatcagc aggtaaaaaa aatgaaagaa
     2461 aaataaaaag atcaggatcc gctttatcca tctccaggta cgcggcacca atcctttggt
     2521 ttttattgcc atcaattctt ttcgttaatg gggatttccg ggcggatggg cgatccggct
     2581 gcccgggtta tccaaatgga ggtcaaggta atgcaatcca tatttctttc cgagcggata
     2641 ggttttgatc cttgattttc tcaataaaat tcctcccgcg atcaggacgc cggccgttat
     2701 ccatccggtt gtggagaacc agtctttcgc cttatccttc cggtagaggc cattcacggt
     2761 atttaaaacg atcactccca cgcccgcaat gatcagcagg gttccgtcct gggagtaatt
     2821 cagtttaccg cgctcggtgc gcatggccct gatctcgtta taatgaaagg gaatatggtt
     2881 gacaaaaacg ctgtcgttcc tcaattcggt gagcatgcca tcgaaccatt ggtcataaat
     2941 ggtctcaaaa ataatacgtt ccccgggtgc ataggtcttg atattccgcc cgtttttctg
     3001 gagaataagc acgtccgtct gggagaaacc ggccaatggg aggagaaaaa gcaaaacagc
     3061 gaagatcttt tgcatacaca aatttaatta cgggcttttc agtcacgtta ccggatccgg
     3121 tgttcaagaa atatgacccc gtaaagcaaa gctattccga aagctatcaa tatggcgccg
     3181 gagattttgt taatgatgtt caaattatga acggtgagcc gttttcttaa ctttccggcc
     3241 atcatcactt ttcccacatc cgccgccata ttgatgagca ggcaggtgga aaaaataacg
     3301 atccgctgct tcaaggtatg cgtaagcgcg agaacggtgg cattgcccag ccagaaagcc
     3361 agaacacccg gattcagggt attgatgaga aacccggaag cgaaagtctt cagcatgtca
     3421 tgaacgctga aatcgtccag gtgcatggac cccgtggtag ccttcagatg aaccttcttg
     3481 aagaaaaaga aataaacgcc aagcgcaatc agaaaactgc ttcctccgta gccaatgagt
     3541 tttttatatt cgagtaagcg ggtgacccat tccgtaaata cattgctgag aactaccaga
     3601 ataacatcgc tcagccagac cccggcaaca aagctgaaac cacccttatg cccactggtg
     3661 atgctttgct tgataatggt aaaaataatc ggacctacag ataatgcaag cacaatacca
     3721 agcgctaatc ctttccatat cgcttcgatc ataggcttta aattttacgg gcactatgcg
     3781 aaaatagcgg aacccggaaa atagatgaac cattttcatt ttaaatgctg attaatactg
     3841 aattttctct gatattttat ccagaaaatt taaccagtcc tttaacattt ttccttttaa
     3901 taccctggga aacttatgct ggccgccctc ctttccactg gcgcgcatga actccatgaa
     3961 cacctccgga gtcagcactt tcacccggat atctttcagt gcgctttttc tctccacggc
     4021 atagtcgtcg ttaagccgtt tcagattttc gtcaattcta tttcggatga gatccggatc
     4081 aagcccggtc tcactggcaa tataccattg atgtgcaaag aacaggccat gcggtatgcc
     4141 ggttacggta aattccggaa tggagatatt gaattcctgg ctcaccagtt gaattgcctg
     4201 gttcatattg tccacactga gatgttctcc caccaggctc agataatgtt tggtgcggcc
     4261 ggtaatgata atctcgcttt tctccttatt ggtgaaccgt attgtatccc cgatcaggta
     4321 gcgccaggcg cccgcacagg tgcttatcaa aagcgcatag tcctttcctt cttccacttc
     4381 attgatctga agggcagcgg ggtctttcac aatatttcca tcctgatcaa aattactgct
     4441 gtcgaatgga acgaattcaa agaacaggtg ctggttggtg accagccgca tccctcgctc
     4501 atcctgccgg tcctggtagg caataaatcc ttccgaggcc aggtaggttt caatataaat
     4561 tattggttta ccgagcagtt tttcaaatcc tcttttatag ggctcaaaag aaacaccgcc
     4621 gtgaacgaaa aagccgaggt tgggccagat ctcgtgaata tttttcagct gatatttttc
     4681 aatgatcttt tccatgcaca tctggatcca ggccgggacg cctacaataa aaccaatatc
     4741 ccagttcggc gcctgctcta cgatctcttc cagtttccgg ttccagtcct tcgtttttgc
     4801 aatccgcttg ccgggtttat aaaaagggga gaaccagaag ggaacttttt ttgcagagat
     4861 cccgctgagg tcgccttcat aatacccggg tcctttctgc agatccgtgc tgccgccgat
     4921 catcagccat ccttttccaa tgcttttaac ggggatgtcc tgataattgc ggaggctcag
     4981 caactgccgg accatgacca cccggttgcc ccgcagcagg tcgttggtca ccggaatata
     5041 tttactggcg gcctccgaag ttccggaact gagcgcataa tattttatct tccccggcca
     5101 gcagatatcc ggtttccctt ccagcgtttt ataccaccac tctttgtaga tcttgctgta
     5161 atcgtagacc ggaacgttct cctgaaaatt tttcaggatc cgtttaccga gcaaaatccg
     5221 gtcaaaatga tattgctgcc caaattcggt gaagcgcgct tttttcagta atttcttgag
     5281 gaccttcaat tgctggcgcc gggctgattt tttcggcaac cgcaacgccc tggcaagcga
     5341 gttgggtaat tgtacatcaa ttaacgccat tgaaatcgga aggtattgat tgtcttcaaa
     5401 attaagggat tgccgacaca tcctgcattt ccggccccgg tatttacggg ttgcttaccg
     5461 ttaccggata gagtggaaca ggctcgcttt cattatttgt tttgctgatg gccgttacga
     5521 gatatcgttc gtttctcttt ccctgcagat cggccacctt gaacagcagt tttggatctt
     5581 cattatccgg aataaaaaga aatacccgtt ccgaatcgat attgaagcgg ccgctggaat
     5641 ccgttggaaa acggccggtg gtcttataga tcgcaaatcc acgcagcgtg tctgaagggc
     5701 ttcctttttc aaaatgaaat accagggaat cccgggtatc atttatcctt aaaaccgggg
     5761 ccgccggttt tgtactgtcc agccagggca ggggggcgat cagcgccggg taattataat
     5821 aatgctgctg aagactatca ttccacccat acggattccg gtaaaaggag gtgctgctga
     5881 aatagacttc tccttgaata ttttccgtgt tccggatagc agtgagctgg cggggcaatt
     5941 gattcttatc gcgccagtag atgcttgaac ccgcccggta atacccgagg ccgatatagc
     6001 aggggcggcc ataactgtgt ttgctccacc agttcagcaa aacgccgaaa ggggcattct
     6061 tatattcaaa ctcccagtaa agctggggca ccacataatc gatccagccg tttttcagcc
     6121 acaataaaat attcgcatag aggtcatcat aattggtctg accggcatgc gtgtcgctgc
     6181 cggcggtatc ctgggaaagg tttctccaga ccccaaaggg actgatcccg aacttgcagg
     6241 ttttcttttc ttcatgaatg gcccggctga gctgggagat gaccgagtcc acattgctcc
     6301 ttctccagtc ctccttgttc ataccattcc cgtatttcag ataagaagca ttgtcgggaa
     6361 attccttgcc cgcgatccgg tagggataaa aataatcgtc gaagtggatg ccatcaatat
     6421 catatctccg caacatgtcg cggaccaccg aaaccacgaa ggcctggact tccttatttc
     6481 ccggatccag gtatttggta tccccatagg tgatcaccca gtccggatga acgcggctga
     6541 tgtggttggc ggcaatggaa gaacgcccga tgcggaatac ggcccggtag ggattgcacc
     6601 aggcatgaaa ctccatgcct cttttatggg tttcccggat catgaactcc agcggatcgt
     6661 aatagggtga cggagctttt ccctgccggc ccgtcagcca ctcgctccag ggttcaagcg
     6721 aagagggaaa aaaagcatcg caagccggtc ttacctgcac cacaacggca ttcatgccat
     6781 tgcgccggtg catgtccagc tgacggataa attcctcttt ctgaagttcg gtattgtaat
     6841 ttccccggga aggccagtcg atattatcca cggtggcaat ccagactgcc cggaattcat
     6901 atttcggctg accggatacg atcaatgaaa acaaaacggg tatgacagga aaaaaaaatc
     6961 ttttcataat taaacacact ggagatggaa tgaaggcgca aaagtaatga tctatccttc
     7021 tttttctgca gacctgatct tatctaacaa ttcatttagc tttcccgcgt cctcttcgct
     7081 gagctgctgc agtactgcat caaattcggt attcagcgta tccatctgct gcaaaagttt
     7141 tttccctttg tcggtcagca gaacatccac caggcgccgg tcctcgtgac acaccgattt
     7201 tttaaccagc ccttttttaa tgagccggtc gacaatgcgg ctggtgtcgc tcattttgtc
     7261 cagcatgcgt tgcctgatct ggagcgtcga cagcggagtt cccgctccgc gcaggatcct
     7321 cagaatatta aactgctgct gcgttacttc aaaagggtca aaacttttgt tcaatctttc
     7381 attcatccag ttgaaggtgt agatcaggtt taaaaccgct ttctggtatt cattgcggaa
     7441 cacatgctgg tttatatcat cttctaaagt cattcgatgc ttaattgaat aaaactaatg
     7501 ttttcccggg tattcttccc cggggacggt ttgcc
//
LOCUS       DASYCU010000464         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4013056, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000464 DASYCU010000000
VERSION     DASYCU010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4013056"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1163
                     /locus_tag="VGM24_09375"
     CDS             3..1163
                     /locus_tag="VGM24_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEY4155629.1"
                     /translation="MDVIGWGLIIPVMPQLISGLKNIPVNQASREGGLLLFVYAFMQF
                     LCAPILGNLSDQYGRRPVLLFSLFGFGVDYIFMGFAPSFGWLFLGRTISGITGASFTT
                     ATAYIADISDEKTRAKNFGLVGAAFGLGFIIGPGIGGLLSGWGIRAPFYAAAILTLLN
                     WLYGYFLLPESLSMEHRRPFSWKRSNPFGGSLMHLRRYPALGGLILALALVYLGSHAV
                     QSNWSYFTMYRFKWTAKTVGISLAVVGALVGLVQGVLIRWITPRIGNEKSIYIGLLLY
                     AAGMFLFAFSTQSWMMFVFLVPYCLGGIAGPALQAIMSGHVPPNAQGELQGALTSVMS
                     LTAIVGPLLMTNLFAYFTSAKAPVLFPGAPFLLGGLLMLSSSVVAYKSLKQN"
     gene            1322..1639
                     /locus_tag="VGM24_09380"
     CDS             1322..1639
                     /locus_tag="VGM24_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY4155630.1"
                     /translation="MNIYVSNLSFNVTDEDLNEYFAEYGEVSSAKVILDKFTQKSRGF
                     AFVEMADEEAAKKAIQELDGASVDGRTINVSVAKPREERTGGAKGGGGNFQKRNNFSN
                     SRY"
     gene            complement(1704..1982)
                     /locus_tag="VGM24_09385"
     CDS             complement(1704..1982)
                     /locus_tag="VGM24_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155631.1"
                     /translation="MKKWLFAAFAGSLLALGGCSTEVVATRPHDVVYSRPVSPGPDYV
                     WVTGDWVWAGGSYRWREGRWARRRSGYQWHEGYWENHNHGWRWRRGHW"
     gene            2237..3277
                     /locus_tag="VGM24_09390"
     CDS             2237..3277
                     /locus_tag="VGM24_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY4155632.1"
                     /translation="MISEAGPALHPLTARDFATDQEVRWCPGCGDYSILAQVQKVLPQ
                     IGVPRENIVIISGIGCNSRFPYYMNTYGMHSIHGRATAIASGLKASRPDLSVWIVTGD
                     GDSLSIGGNHTIHLLRRNFNVNILLFNNQIYGLTKGQYSPTSETDKMTKSTPFGSIDH
                     PFNPLALAMGADASFVARSMDRDPKHLQQMLMRSHQHIGSSFLEIYQNCNIFNDGAFE
                     IFTEKGSKPVETIFLEQGKPLIFGAERNRGIRLDGLRPVVVEIGNGIQAEDLWIHDEK
                     DFYKAQILVRMFDDPKPDAHLPRPFGVFYETERPCYEDMLNLQIEEVIRSRGKGDLNK
                     LLRGRETWTISA"
     gene            complement(3700..>4376)
                     /locus_tag="VGM24_09395"
     CDS             complement(3700..>4376)
                     /locus_tag="VGM24_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY4155633.1"
                     /translation="FGEYIDLIRRGPAEWRIFLFNLFDHAPQIIRDFSWPEQYSKGFV
                     KKFPMLFTGGQGSITHMHFDIDLSHIFHTQFMGKKRVLLFPYEEQYKLYRKPWEVLSL
                     VNFERYFDGAHNKLDLQKFPALRLARGYELILGHGDTLFMPAGYWHHMEYIDSGFAMS
                     LRAMQSGLSGKLKGAWNLFGMRSLDTLLKKTAPEWWYRYKKDKTFADAGRAMETYGAN
                     PAAGFS"
BASE COUNT         1060 a         1097 c         1090 g         1129 t
ORIGIN      
        1 tgattgatgt catcggctgg ggactgatca ttcccgttat gccccagctc atctccgggt
       61 taaaaaacat accggtgaat caggccagcc gggaaggcgg gttgctgctt tttgtctatg
      121 cttttatgca gttcctctgc gccccgatac ttggcaacct gagcgatcag tatggacgcc
      181 ggcccgtttt gcttttttcg ctgttcggct ttggagtcga ctatattttc atgggctttg
      241 ctcccagttt cggctggtta ttcctgggca ggaccatatc cgggatcacc ggcgccagct
      301 ttacaacggc caccgcctac attgcagaca tcagcgatga aaaaacaagg gcaaaaaatt
      361 tcgggttggt gggtgccgcc tttggactgg gctttattat cggtccggga atcggcggcc
      421 tgctgagcgg ttggggaata cgggccccgt tttacgcggc agccatcctg accctcctca
      481 actggttgta cggatatttt ctgcttccgg aatccctgag catggaacac cggcgcccgt
      541 tcagctggaa aaggtcaaat ccttttgggg gttccctgat gcacctgagg cgctatccgg
      601 cactgggcgg tctgatcctg gctctggccc tggtctatct cggttcccat gccgttcaaa
      661 gcaactggag ttattttacg atgtaccgct ttaaatggac ggccaaaaca gtaggtattt
      721 ccctggcggt ggtcggcgcc ctggtcggac tcgtgcaggg agtgctcatc cgctggatca
      781 ccccaaggat cggaaacgaa aaaagcattt atatcggatt attattatat gccgcgggca
      841 tgtttctgtt tgccttttca acgcagagct ggatgatgtt tgtattcctg gtcccttatt
      901 gtctgggtgg gattgccggc ccggccctgc aggccatcat gtcgggccat gtgccgccca
      961 atgcacaggg tgaattgcag ggcgcactga ccagcgtaat gagccttacg gccatcgtcg
     1021 gacctttgct catgaccaat ctctttgctt atttcaccag cgcgaaggcg ccggttcttt
     1081 tcccgggagc accctttctg ttaggtggtt tgttaatgct ttcgagcagc gtggtggctt
     1141 ataaaagttt aaaacagaat tgaaaatccg gaatatatac ctatatttgc cccgttaagt
     1201 tgataggcat ttagtaagtg aacaaacatt ctgctacgca atcttcagta cggttagtta
     1261 gtcttcttta ctcctgcttt ttgtcagctg tatttttatc atttaatctt ttattttaag
     1321 tatgaacatt tatgtttcaa atttaagctt caacgtaacc gatgaagact taaacgagta
     1381 ttttgcagag tacggtgaag tttcatctgc taaggtaatt ctggacaaat tcacccagaa
     1441 atcacgtgga tttgcatttg ttgaaatggc agacgaagaa gctgcaaaaa aagcaatcca
     1501 ggaactggac ggcgcttccg tggacggcag aacgatcaac gtatcggtcg ctaaaccccg
     1561 cgaagaacgc accggtggcg ctaaaggcgg cggcggtaat ttccagaaga gaaataattt
     1621 cagcaacagc agatattaat cgcttttgat tcaaaaaagc cgttgatccg ggtcattccg
     1681 cgatcaacgg ctttttttat agtctaccag tggccccttc tccacctcca tccatggtta
     1741 tgattttccc aatacccctc gtgccattga tatcctgacc tgcgccgggc ccatcggcct
     1801 tcccgccaac ggtagcttcc gcccgcccac acccagtcgc cggtgaccca tacataatcc
     1861 ggtccgggcg aaaccggtct gctataaacc acgtcgtgag gccttgtggc caccacttct
     1921 gtactgcagc cgccgagggc gagtaacgac cctgcaaatg cggcaaataa ccattttttc
     1981 attttcctgt tatttaagta tatccaatcc ggtattagta aagcaaaacc cgttctgaat
     2041 aagaccgggg tcggcccggg aagtaaaaac caaatcataa gaatctgaat acccgaccct
     2101 taggggagga tgaacaaaaa agcgttaact ttttattttt tacgccctat ccgttttgtg
     2161 taggcctttc tacaggaatt gctaaaatcg gaacttaact ggcggggata tacttaattt
     2221 gcaaaaaact tatttaatga tcagtgaagc cgggccagcc ctccatcccc tgacagccag
     2281 ggattttgct accgaccagg aagtgcgctg gtgtcccggt tgcggcgatt attccatcct
     2341 ggcccaggtg caaaaggtat tgccccagat tggcgttccc cgcgagaata ttgtgattat
     2401 ttcggggatc ggctgcaata gccgtttccc ctattatatg aatacttatg gcatgcactc
     2461 cattcacggg agagcgactg ccattgcctc cggtctgaag gccagccgtc cggacctgag
     2521 cgtatggatc gttaccggag acggggattc cctgagcatt ggcggaaacc ataccatcca
     2581 tctgttgcgc cggaatttta acgtgaacat tctcctgttt aacaaccaga tctacggact
     2641 taccaaaggc cagtattcac ccacttccga aacggacaag atgaccaagt caaccccatt
     2701 cgggagcatt gaccatcctt ttaatccgct ggccctcgcc atgggggcgg atgccagctt
     2761 tgtagcccgg agcatggacc gggatcccaa acacctgcag caaatgctga tgcgcagcca
     2821 tcagcacatc gggtcctcct ttctggagat ctatcagaac tgcaatattt tcaatgacgg
     2881 cgcctttgaa atttttacgg agaagggctc caaaccggtt gaaacgattt tcctggaaca
     2941 gggcaagccc ctgatcttcg gggcagagag gaatcgcggc atccgtctcg acggcctccg
     3001 gccggtagtc gtggaaatag gcaatggcat ccaggcagaa gacctttgga tccatgatga
     3061 aaaggatttt tataaggccc agatcctggt caggatgttt gacgacccga agccggacgc
     3121 gcatctaccc cgtccgtttg gggtctttta tgaaaccgaa cgcccctgtt acgaggatat
     3181 gcttaatctt cagattgaag aagtgatcag atccaggggt aagggagacc tcaacaagct
     3241 gctgcggggc cgcgaaacct ggaccatctc cgcttagcct cttccggatt tctataaaaa
     3301 aagcccgccg tagcgggact ttttattggt tgtttcacgg agaaatgcgt ttaggaaata
     3361 aaaatgccgg cgataaaaat cgccggcctg agcttacctc tgaaaccaca aaaagaaaac
     3421 gttaagggca atcattgaat tattcatcgt tgagacctgt aatcgttaaa gacctgtcca
     3481 caataatcgt tggaaagatt ggatgtattt atccgaaaac accggttcag tcgattttac
     3541 agggtcagga aaagcatatc cgtccggatg agtgttgaga tcacgggatg gcatcagaaa
     3601 acggaaattg gacagggacg ggtttaggaa aggctgccaa ggcagcagca gattaaaagt
     3661 aactacaagt ttcctattag caaagcgttt tttttaaatt caggaaaaac ccgcggccgg
     3721 attcgcgcca taggtttcca ttgcccttcc ggcatcggca aaagtcttgt ctttcttata
     3781 ccggtaccac cattccgggg ccgttttttt caacagggta tcgagggatc gcatgccaaa
     3841 aaggttccag gctcccttta gtttcccgga tagcccggat tgcatggcgc ggaggctcat
     3901 ggcgaaaccg ctgtcaatat attccatatg atgccagtac ccggcgggca taaacagggt
     3961 atccccatgg cccagaatca gttcataacc cctggccagc cggagggcgg ggaatttttg
     4021 caggtccagc ttgttgtgtg cgccgtcgaa atacctttca aaatttacca ggctgagcac
     4081 ttcccagggt tttctgtata atttatactg ttcttcataa gggaaaagca gaacccgctt
     4141 ttttcccatg aactgggtat ggaagatatg ggagagatcg atatcgaaat gcatgtgggt
     4201 aatggaaccc tggcctcccg taaacagcat ggggaatttt tttacgaatc ccttactgta
     4261 ttgctccggc cagctgaaat cccggatgat ctggggggcg tgatcgaaga gattgaataa
     4321 gaatatccgc cattctgccg gccccctccg gatcaggtct atgtattccc cgaatt
//
LOCUS       DASYCU010000465         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_535342, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000465 DASYCU010000000
VERSION     DASYCU010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535342"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..>2538
                     /locus_tag="VGM24_09400"
     CDS             246..>2538
                     /locus_tag="VGM24_09400"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY4155634.1"
                     /translation="MELSKHYDPAPTEARWYAYWNEKHYFNSIPDQREAYTIVIPPPN
                     VTGVLHMGHTLNETVQDILVRRARMSGFNACWVPGTDHASIATEARVVAMLKEKGIEK
                     NSLSRESFLKYAFDWKDKYGNIIYDQIARLGCSVDWNRVSFTMDDHYYKAVIQVFVDL
                     YRKNLIYRGARMINWDPAARTALSDEEVEYRDVEGTLYYIKYPVAGTSEFIPIATQRP
                     ETIMGDTAVCVNPDDPRYQHLKGKQVMVPLVNRKVPLIFDDYIDMAFGTGALKVTPAH
                     DINDYKLGLKHGLPVIDTLNEDGTLSAAAEIWVGLDRFEARKKVVEKLREEGLLWKEE
                     KYSTRLGYSQRSQAVVEPRISTQWFVRMKPLAEPALNQVLSGEIRIHPGEKFLATYKY
                     WLENVKDWCISRQLWWGQRIPAWYDGKRNFVVAENEADAMEVYQKEFGLLEAGQKLVQ
                     DEDVLDTWFSSWLWPMEVFRGITAPGNPEIDYYYPGAVLVTGQDILFFWVARMIMAGM
                     EYRKEKPFSDVYFTGMVRDKQGRKMSKSLGNSPDLLQLIEKYGADAVRFGILISSPAG
                     NDLLFDEAGLEQGRNFNNKLWNALKLLNNWRMKQDPGTAPAEGAGETEIAPGDHFAVQ
                     WFENRLNQAKKDIQKLISEFKLSEALKKLYSLAWDDFCSWYLEWIKPGYGQSMAPELY
                     GKTISLFDELLQWLHPFMPFVTEEIYHLLKEREPGDDLCIKQFRAPGETDPDLLEKGD
                     RLRMLLTAGREFRQQHQLKNSETI"
BASE COUNT          677 a          605 c          678 g          578 t
ORIGIN      
        1 ttgcttttgg atttcttgcg ctggtcatgc tgctttcata tctgtactgg tttgcataga
       61 gttccgctta ccagctgaat ctgaattcag aacctctatt cccgaaatac gagaggataa
      121 gttttatatc cctctcctaa gagaaaagtt tcctatccga cggaaacttt tttatttgga
      181 tccatccgct ctcccatcct tctaagcgat tgattaactt tgccctcctc acaaaatgca
      241 tttggatgga actgagcaaa cattatgatc ctgcccccac agaagccagg tggtacgcct
      301 actggaacga aaaacattat ttcaacagca taccggatca gcgtgaagcc tatacgattg
      361 tgatcccccc gccgaatgtg accggagtgc ttcatatggg gcataccctc aatgaaaccg
      421 tccaggatat attggtgcgc cgcgcccgca tgagcgggtt caatgcctgc tgggtgcccg
      481 ggaccgatca cgcgtccatt gccacggaag cccgggtggt ggccatgctc aaagaaaaag
      541 gaattgaaaa gaacagcctg tcccgcgagt catttcttaa atatgccttt gattggaaag
      601 acaaatacgg gaatatcatt tacgatcaga tcgcccggct gggctgcagt gtagactgga
      661 accgggtcag cttcaccatg gatgatcatt attacaaagc cgtcattcag gtttttgtgg
      721 atctctaccg gaaaaatctg atctatcgcg gcgcgcggat gatcaactgg gacccggccg
      781 cgcgcactgc actaagcgat gaggaggtag aataccggga tgtggaaggc actctttatt
      841 atataaaata tccggttgcc ggaaccagcg aatttattcc cattgccacc cagcgccccg
      901 aaaccatcat gggagacacc gcggtgtgcg ttaacccgga cgatccccgg taccagcacc
      961 tgaaaggaaa acaggtgatg gttccactgg tgaaccggaa agtacccctg atcttcgacg
     1021 attatattga tatggccttt ggaacgggcg ctctgaaagt gacgccggcc catgatatca
     1081 acgactataa gctcgggctt aaacacgggc taccggtaat cgacaccctg aacgaggacg
     1141 gaacgctttc cgccgctgcg gaaatatggg tgggcctgga ccggtttgaa gcccggaaaa
     1201 aagtggtgga aaaactcagg gaagaaggat tgctctggaa ggaggaaaaa tattccaccc
     1261 ggctgggtta ttcccaaaga tcccaggctg tggtggaacc gcgcatatca acgcagtggt
     1321 ttgtaagaat gaaaccgctg gcggaaccgg cgctgaacca ggtcctgtcc ggggagatcc
     1381 ggattcatcc gggagaaaaa ttcctggcca cctataaata ctggctggaa aatgtaaagg
     1441 actggtgtat ttcacggcag ctctggtggg gtcagcggat acccgcctgg tacgacggga
     1501 aaagaaattt tgtcgttgcg gaaaatgagg cggatgccat ggaagtctat caaaaagaat
     1561 ttggtctcct ggaagccggg caaaagctgg ttcaggatga agacgtgctc gacacctggt
     1621 tttcttcctg gttatggccc atggaagtct tccggggaat aacagcaccg ggaaacccgg
     1681 aaatcgatta ttattatccg ggagcggtgc tggtaacggg gcaggacatc ctgtttttct
     1741 gggtggcccg gatgatcatg gccggaatgg aatataggaa agaaaaacca ttttcagatg
     1801 tgtacttcac cggaatggta agggataaac agggcagaaa aatgagcaag agcctgggga
     1861 attcacccga tctcttacag ctcattgaaa agtatggcgc cgatgcagtc cggtttggta
     1921 tcctgatctc ttcccccgca gggaatgatc ttttgtttga cgaagccggt ctggagcagg
     1981 gaagaaattt taataataaa ctatggaatg ccctgaagct gctgaacaat tggagaatga
     2041 agcaggaccc cgggaccgcg ccagccgaag gcgccggtga aacggagatt gctccgggtg
     2101 atcattttgc cgttcagtgg tttgaaaacc ggctgaacca ggcaaaaaag gatattcaga
     2161 aattaatttc cgaattcaaa ctgagcgagg cgctgaaaaa actttattcc cttgcctggg
     2221 acgatttctg cagctggtac ctggagtgga tcaaacccgg atatggacaa tccatggcgc
     2281 cggaattata cggcaaaacc atttccctgt ttgacgaatt gctgcagtgg cttcacccgt
     2341 ttatgccctt tgtaacggaa gaaatttatc atttgctgaa agaacgcgaa cccggcgacg
     2401 atctttgcat taagcagttc cgggctccgg gagaaacgga tccggacctc ctggaaaaag
     2461 gcgacaggct tcggatgctg cttaccgccg gacgcgaatt ccgccagcag catcagctga
     2521 aaaacagcga gaccataa
//
LOCUS       DASYCU010000466         5860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4595802, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000466 DASYCU010000000
VERSION     DASYCU010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4595802"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VGM24_09405"
     CDS             complement(<1..1342)
                     /locus_tag="VGM24_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155635.1"
                     /translation="MEKRVSMNPNGEKDCLFNGNRFPDHASTMQIDQTTLADLSVFSP
                     ADEYSLFNKLDLTRTVGGRIQLFQLFNQPFSSLDKIRETQQILAIIQRNENAWGKSIS
                     NGTVMVMERFYETALDKIPAGYDPVTAGSYKLFHSPDYSLTRYSIEHFADFIRGMKSI
                     CAVLETEDCPPMLAGLLERVRRLLDQQILQSLAERKPGKRLRATEVVQYGYFIRNRFK
                     TAALDLIAIYGKLDAWYSMAAATRQFRLAIPEFTDREDPYLDLAALYHLLLPEPVSYD
                     LKMDHGCNFVFLTGANMAGKSTFIKAVGCALFLAHLGMGVPADNMKLSLFDGILSNIN
                     VADNLSKGESYFYNEVQRIKKTILLIRDQKKWLVLIDELFKGTNVEDAMRCSSVVIKG
                     LIRIRTSLFILSTHLYEIGEELKTYPNISFRYFETNVEDEQLVFNYQLRNGISND"
     gene            complement(1284..2672)
                     /locus_tag="VGM24_09410"
     CDS             complement(1284..2672)
                     /locus_tag="VGM24_09410"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY4155636.1"
                     /translation="MQNYLIKNISIVNEGLVFNSDLLIKNGRIEQIAGRISTSAGVEE
                     INGEGLHLLPGCIDDQVHFREPGLTHKADIHSESRAAVAGGITSFMEMPNTLPPAFTQ
                     ELLEDKYAIASASSLANYSFYMGTSNDNLSEALRTNEKKKEVCGIKIFMGSSTGNLLV
                     DNYLTLDKLFAGIEVLIATHCEDENIIRRNLQRFQSADRIPVPADHPVIRNEEACFQS
                     SLTAIQFAKKHGSRLHVFHLSTEKEMQLFSNLIPLAEKRITAEVCVHHLHFTADDYGR
                     KGNLIKCNPAIKPANNREALWKALLDDRLDLIATDHAPHTLKEKGFFRNETGELEKLS
                     GEPGDYFHAHAGLPLVQHSLLLMLHYVKEGRISLEKVVEKMSHAVATCFQIRERGFIR
                     EGYFADLVMVDLKGTSDIHAGNILYKCGWSPMEGMHFPASIINTFVNGHKVYGKEGFD
                     ESQRGKRLSFQR"
     gene            2702..4408
                     /locus_tag="VGM24_09415"
     CDS             2702..4408
                     /locus_tag="VGM24_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155637.1"
                     /translation="MVQFIKTVARHVIILALPVFGFSQSTFLPEGSQYEHFLDRLTIL
                     LQNNPDLNLATARPVSRKLAVEVSEMSDSLDKFYPYDDFYHLSKTDRATLQSLLMNNS
                     EWVTGSKASFQSKHPLWNTIYKTRANFLEVNEKDFFLAINPVIQQQQSYETGNHEHVF
                     LNSKGLTARGLIANHLGFSTYITDNQERGPSFFQDRVFASGFPAVPGAGYFKDFKKTA
                     FDYFDARGTINVDFWKYFNVQFGYDKNFIGDGYRSLFLSDYSAPYLFLKFNTRIWKLN
                     YEDIFMELISQHQRGDYQYPKKYAVVHHLSVNASKWLNIGLYDNVVFSRADYFDFSYL
                     NPVIFLVAAQQENGSPDKTTVGLDFKANIGHATQLYGQLLINEFVLHEVLHYSNGWWG
                     NKQGLQLGLKYIDAFSVKNLDLQFETNLVRPYTYSHNDTVSNYSHYNQPLGHPLGSNF
                     IEFIGIARYQPVYKWNMELKCIFYRQGLDTGGVNYGGNIFLDYTTRPRDYGFGLPSGV
                     PATCLNASAYVAYQWKENLFLEGSMMYRSYTRHEPLSTKTSSTTFTLGLRLNMFRRIY
                     DY"
     gene            complement(4436..5182)
                     /locus_tag="VGM24_09420"
     CDS             complement(4436..5182)
                     /locus_tag="VGM24_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4155638.1"
                     /translation="MDLLHFRRNKKNLYGVLSFSFVLIFSTSARAQLRDGINLPDHDS
                     KFYHLGIALIYNTSHFQVESHPSFLQSDSVLSVNPENTGGFGLAGMHTFKFSPHLEFR
                     IVFPQLMFSYKNLTYSIKYPGPGEKAIATKRVESILLGFPIQAKFLSDRINNFRVYML
                     GGVNYQYDLASNATSRKADDLVKLKPYDFSIEAGFGFQFFFPVFILSPEIKISNGIIN
                     SHSRDPNLIYSSSIDKLRSRMIVFSLIFEG"
     gene            complement(5346..>5860)
                     /locus_tag="VGM24_09425"
     CDS             complement(5346..>5860)
                     /locus_tag="VGM24_09425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEY4155639.1"
                     /translation="LMTKKLNPEKITGIDISGGMLELGKQKINRLGLANRISLLPGDS
                     ENIGFPTASFDAVTVAFGVRNFENLEKGLLEMHRVLKPGGKLMALEFSKPQKGLFLPF
                     YKIYLRHIAPRIGRIVSGNPDAYQYLNDSVNAFPEGDLFTGILEKAGFDNSYCKKLSL
                     GICSIYCGSK"
BASE COUNT         1489 a         1415 c         1408 g         1548 t
ORIGIN      
        1 ggtcgttact gatcccgttc ctgagctggt aattgaatac cagctgttcg tcttccacat
       61 tcgtttcaaa ataccggaaa gaaatattgg gataggtttt cagttcttcc ccgatttcat
      121 acaaatgggt ggaaagaata aaaagggaag tcctgatcct gatcaggcct ttgattacaa
      181 cagaagaaca cctcatcgcg tcttccacat tggtcccttt aaacaattca tcgatgagca
      241 cgagccattt cttttgatcc cggatcagca ggatcgtttt ttttattcgc tggacttcgt
      301 tataaaaata actttcaccc tttgataaat tatcggccac attaatattg ctgaggattc
      361 cgtcgaacag gctgagcttc atgttatctg caggaacgcc catgccgagg tgggcgagaa
      421 agagggcgca gcctaccgct ttgatgaagg tgctcttacc ggccatattg gctccggtca
      481 ggaatacaaa attacatccg tgatccattt tcaaatcgta ggataccggt tccggaagta
      541 agagatggta cagggcggcc agatccaggt aaggatcctc ccggtccgtg aattccggaa
      601 tggccagccg gaattgccgc gtggccgccg ccatcgaata ccaggcatcg agtttcccat
      661 agatggcgat cagatccaac gctgcggttt taaaccggtt ccggataaaa tatccgtatt
      721 ggacgacttc cgtggcacgg agtcttttac ctggttttct ttccgcaagg gattggagga
      781 tttgctgatc gagcagacgc ctgacccgtt ctaatagtcc ggccagcatc ggtgggcaat
      841 cttcagtctc caatacagcg cagatggatt tcattccccg gatgaaatcg gcaaaatgct
      901 caatggaata cctggtcagg gaataatcgg gcgaatggaa taatttatag gagccggcag
      961 taaccggatc atagccggcc ggaattttat ccagcgcggt ttcatagaat cgctccatca
     1021 ccatcaccgt tccgttggaa atggattttc cccaggcatt ttcattccgc tgaatgatgg
     1081 cgaggatctg ctgggtctcc cggatcttat ccaggctgct gaacggctga ttgaagagct
     1141 gaaacagttg tatccttcca ccgaccgttc tggtcagatc cagtttgtta aacagggaat
     1201 attcatctgc cgggctgaac acagacaggt ccgccagggt cgtttggtct atttgcatag
     1261 tcgaagcatg atccggaaac cggttaccgt tgaaaagaca gtctttttcc ccgttgggat
     1321 tcatcgaaac cctcttttcc ataaactttg tgcccgttta caaaggtatt tatgatggaa
     1381 gcggggaaat gcattccttc catcgggctc cagccgcatt tatacaggat attaccggca
     1441 tggatatccg aagttccctt cagatccacc atcaccaggt cggcaaaata tccttcccgg
     1501 ataaatcctc tctcccggat ctgaaaacag gtggctaccg catggctcat cttttcaacc
     1561 actttctcca gggatatcct tccctccttt acataatgca gcatcaataa cagggaatgt
     1621 tgtaccaggg gcaggccggc gtgggcatgg aaataatcgc cgggttctcc gcttagtttt
     1681 tcgagttcac cggtttcatt ccggaaaaac cctttttctt tcagggtgtg cggggcatga
     1741 tccgtggcga taagatccag gcggtcatcc agcagggcct tccacaaggc ttcccggtta
     1801 ttcgccggct tgatggccgg gttgcacttg atcaggttgc ccttccggcc atagtcatcc
     1861 gctgtaaaat gcagatgatg cacacagact tccgcagtga tccttttttc cgccagggga
     1921 atgagattgc tgaataactg catttccttc tcggttgaaa gatgaaaaac gtggaggcgg
     1981 gaaccatgtt ttttggcaaa ttgtatcgct gtcagcgagg attgaaaaca ggcttcttca
     2041 ttgcggatca cggggtgatc ggccggaacg ggtatccggt ctgcggactg gaaccgctgc
     2101 aggttccggc gaatgatgtt ttcatcttca cagtgcgtgg cgatcagcac ttctattccg
     2161 gcaaacagtt tatccagcgt caggtaatta tccaccagga ggttcccggt ggatgagccc
     2221 atgaatattt taattccgca gacttccttt ttcttctcat tggtcctgag cgcctcgctg
     2281 agattgtcgt tggaagtgcc catataaaaa gaataattgg ccagcgagct ggccgaagcg
     2341 atggcatatt tatcttccag caattcctgc gtaaacgccg ggggaagggt attgggcatt
     2401 tccataaagg aagtgatgcc gccggcaaca gcagcccttg attccgaatg aatatcggct
     2461 ttatgcgtta ggccgggttc acggaaatgg acctggtcgt ctatacaacc gggcagcagg
     2521 tgcaggcctt ccccattgat ttcttctaca ccggcggaag tggaaatgcg gccggcgatc
     2581 tgttcaatcc gcccgttttt tatcagcaga tcgctgttga ataccagtcc ttcgttaacg
     2641 atggaaatat tcttaatcag gtaattttgc atatcttgga tcgaaatcgt aaacagctta
     2701 catggtgcaa tttattaaaa ccgtggcaag gcatgtgata attctggcgc tgcccgtctt
     2761 cggtttctcc caatccactt tcctgcccga aggatcgcaa tacgagcatt tcctggaccg
     2821 tctcaccatc ctgctccaga acaatccgga tctgaattta gcaacggcca ggccggtgag
     2881 ccgcaaactg gcggttgaag tgtcggaaat gtcggactcc ctggataagt tttatcctta
     2941 cgatgatttc tatcatcttt cgaaaacaga ccgcgccacc ctccagagcc tgctgatgaa
     3001 taacagcgaa tgggtgaccg gaagcaaagc ctctttccaa agcaaacatc ccctttggaa
     3061 tacgatttat aaaaccaggg cgaattttct tgaagtaaat gaaaaagact tttttctggc
     3121 gatcaacccg gtgatccagc agcagcagtc atatgaaacg ggtaaccatg agcacgtctt
     3181 tctgaattca aaggggctga ccgcccgcgg gcttattgcc aatcacctcg ggttctccac
     3241 ctatataacc gataaccagg agcggggacc gtcttttttt caggaccgcg ttttcgcatc
     3301 gggtttcccg gcggtccccg gtgccggtta ctttaaagat ttcaaaaaga ccgcttttga
     3361 ttacttcgac gcaagaggta ccattaatgt ggatttctgg aaatatttca atgtgcagtt
     3421 cgggtacgat aaaaatttta tcggcgacgg ataccggagc ctttttttaa gcgattattc
     3481 ggcgccctat cttttcctta agttcaatac ccgcatctgg aagctgaatt acgaggatat
     3541 ttttatggaa ctgatcagtc agcaccagag aggggattac cagtacccga agaaatatgc
     3601 ggtggtgcat catctgagcg taaacgccag caaatggctg aacatcggtt tatatgataa
     3661 tgtggttttc agccgcgcgg attatttcga tttctcctac ctcaacccgg tcattttcct
     3721 ggtggctgcg cagcaggaaa acgggagtcc ggataagacc accgtcggac tggatttcaa
     3781 agcgaatatc ggtcatgcga cccagttata cgggcagttg ctgatcaatg aatttgtgct
     3841 ccatgaagta ttgcattatt ccaatggctg gtggggaaac aagcagggat tgcaactggg
     3901 acttaaatac attgatgcgt tcagtgtgaa gaacctcgat ctccagtttg aaaccaacct
     3961 ggttcgtcca tatacttatt cacacaacga taccgtctcc aattattcac attacaatca
     4021 gcccctgggt catccgctgg gatcgaattt tattgagttt atcggtatcg ccaggtatca
     4081 gccggtttat aaatggaaca tggaactcaa atgtattttt taccggcagg gcctggatac
     4141 gggcggcgtc aattacggcg gcaatatctt cctggattat accacccggc cgcgcgatta
     4201 cggatttggc cttccctccg gcgtgccggc gacctgtctg aacgcatcgg cctatgttgc
     4261 ttaccagtgg aaagaaaatc tgttcctgga aggctccatg atgtaccggt cctatacccg
     4321 gcacgaaccc cttagcacaa aaacaagttc aaccaccttc acgctgggtt tgcgactgaa
     4381 tatgttcagg cggatttatg actattgatt ttaggggacc tgcagcagca cctccttatc
     4441 cctcgaagat cagggaaaag acgatcatgc gggaccggag cttgtcgatg ctgctggaat
     4501 aaatcaggtt tggatcccgg ctatgactgt tgatgatccc gttgctgatc ttgatctccg
     4561 gggaaagaat aaaaacgggg aagaaaaact gaaagccaaa accggcttcg atactgaaat
     4621 catatggctt cagctttacc agatcatccg ccttccggga agtggcattg gaagccaggt
     4681 catattgata attcaccccg cccagcatat acaccctgaa attattgatg cggtccgaca
     4741 gaaatttggc ctggatcggg aaccccagca ggatggactc cactcttttg gtggcaatcg
     4801 ctttttcacc gggtcccgga tatttaatgc tataggtaag gtttttataa gaaaacatca
     4861 gctgcgggaa tacgatccgg aattcaagat gcggactgaa tttaaacgta tgcattcccg
     4921 ccaggccgaa accccctgta ttttcaggat tcacggaaag cacgctatca ctctgcaaaa
     4981 aagacggatg ggattccacc tgaaaatgcg aagtattgta aatcagtgcg atccccagat
     5041 gataaaactt actgtcgtga tccggcaggt tgataccatc ccttaactgg gccctggcag
     5101 acgttgaaaa aatcaggacg aatgaaaaag ataagacccc gtagaggttt tttttgttcc
     5161 gccgaaaatg taataaatcc ataagtaaca gtcaatcaat aacttacagt ttaatttatg
     5221 aaaagtaagg tatcctgcgg aacgggatcc tgataaaatc ctgttcagaa tcaaaataac
     5281 ggccaaaaaa cagaataatt gtcgttatcc ggcttaataa cattcctttg attgctggaa
     5341 aaggcctatt tgctgccgca gtaaatgctg caaataccca aactgagctt tttacaatag
     5401 ctgttgtcaa aaccggcttt ttcgaggatg ccggtgaaaa ggtctccttc cggaaaagca
     5461 ttgaccgaat cgttaaggta ttggtatgca tccgggtttc cggatacgat tcttccgatc
     5521 cggggagcga tatgacgcag ataaatttta tagaacggca gaaataatcc tttctgcggc
     5581 tttgaaaatt cgagggccat cagtttaccg cctggtttca gcacccggtg catttccagc
     5641 aggccctttt ccaggttctc aaaatttcgg actccgaatg ctacggtcac tgcatcaaag
     5701 gaagcagtgg gaaaaccgat gttctcgctg tccccgggca gcagactgat ccggttagcc
     5761 agccccagcc ggttgatttt ctgttttccc agttccagca ttccaccgga gatatcgatt
     5821 ccggtaattt tctcgggatt gagctttttg gtcatcagaa
//
LOCUS       DASYCU010000467         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3725931, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000467 DASYCU010000000
VERSION     DASYCU010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3725931"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VGM24_09430"
     CDS             <1..636
                     /locus_tag="VGM24_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEY4155640.1"
                     /translation="WFLANQHEMASLVSRWGEKGKDEKHLLSLLRGHRMKRILYRMLD
                     ETEFLSEHGVRALSKYHEEHPYVMKVDGVELSVKYTPGESDIPIFGGNSNWRGPVWMP
                     ANYLIIESLKRFHDYYGDDFKVEYPTHSGNYFSLKEIAEKLAERVVSLFTRDQQGKRP
                     IYGNNPLLQSDPYFRDHILFFEYFHGDSGLGLGASHQTGWTALVSHLLPHK"
     gene            676..1674
                     /gene="obgE"
                     /locus_tag="VGM24_09435"
     CDS             676..1674
                     /gene="obgE"
                     /locus_tag="VGM24_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY4155641.1"
                     /translation="MEKSNFVDQIRIFCRSGHGGAGIRHFLRTKYNALAGPDGGDGGR
                     GGHIILRGNKNTWTLLHLRYFKNVLAENGESGSGNKCTGRFGKDIYIDVPLGTIARDE
                     TTGLVEAEILEDGQEIIWLKGGRGGLGNAQFATPTNQAPEHAQPGEEGTEGWKVLELK
                     VLADVGLVGFPNAGKSTLLSVITAAKPKIADYAFTTLSPQLGMVEYRDGKSFCIADLP
                     GIIEGAAEGKGLGHRFLRHIERNVALLFMIPADSPDHRKEFEVLLNELEQFNPELLHK
                     RFVIAISKSDLLDEELITAVEKELPAGIPHIFISSVTQKGIQALKDLLWTIMNEDK"
     gene            1702..>2030
                     /locus_tag="VGM24_09440"
     CDS             1702..>2030
                     /locus_tag="VGM24_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY4155642.1"
                     /translation="MKCIALIPARYASTRFPAKLMQVLGSKTVIRHTYDNTIATALFD
                     EVMVVTDSDLIYQEITSHGGKAKMSRKTHESGSDRIAEAVEDMDADIVLNVQGDEPFV
                     QKKPLEKL"
BASE COUNT          547 a          494 c          538 g          451 t
ORIGIN      
        1 tggtttttag cgaaccagca cgaaatggcc agcctggtct cccgttgggg ggagaaaggg
       61 aaagatgaaa aacatttatt atccctgctg cgcggacacc gcatgaaaag aattctctac
      121 cgcatgctgg atgaaaccga atttctgagc gaacacgggg tccgggcact ttcaaaatat
      181 catgaagagc acccttacgt tatgaaggtc gacggtgtcg agctctcggt taaatatacg
      241 cccggagaat ccgatatccc gattttcggc ggcaactcca actggcgggg acctgtgtgg
      301 atgccggcca attacctgat cattgaatca ctcaaacgat tccatgatta ttacggagac
      361 gatttcaaag tggaatatcc gacccattcg ggaaactatt tttctttaaa ggaaatcgcc
      421 gaaaagctgg ccgaacgggt tgtttccctg ttcacccggg atcagcaggg caaacggcct
      481 atctacggaa acaacccact gcttcaatca gatccctatt tcagggacca tatcctcttt
      541 tttgagtatt tccatggaga cagcggcctg ggcctgggcg cttcccacca gacgggatgg
      601 accgccctcg tcagtcattt actgccgcat aagtaagctc cggaatcagt tatctttgct
      661 gcccgaattt aaaaagtgga aaaaagcaat tttgtagacc agatccggat tttttgccgc
      721 agcggacatg gcggcgccgg tatcaggcat ttcttacgca ccaagtacaa tgcattggcg
      781 gggccggatg gcggagatgg tggccgtggc ggccatatca ttctccgggg gaataaaaac
      841 acctggaccc ttcttcatct gcgctatttt aagaacgtgc tggccgaaaa tggggaatcc
      901 ggcagcggaa acaaatgtac cggccgtttc ggaaaggata tttatatcga tgtgcccctg
      961 ggcaccattg cccgggacga aacgaccggt ttggtagaag ccgagatcct ggaagacgga
     1021 caggagatca tctggttgaa aggcgggcgc ggcggattgg ggaatgcgca gtttgcaacc
     1081 cccaccaatc aggcccccga acatgcccag ccgggcgaag aaggaacgga aggctggaag
     1141 gtcctggaac tgaaagtgct ggcggatgta ggtctggtcg gattcccaaa tgccggcaag
     1201 tcaaccttgc tttccgtaat taccgctgcc aaaccaaaaa tagctgatta tgcatttacc
     1261 acgttaagcc cgcagctggg catggtggaa taccgcgatg gaaaaagctt ttgcattgcc
     1321 gacctgcccg gaatcatcga gggcgcagca gaaggaaagg gactgggcca tcgcttttta
     1381 cggcatattg aacgaaatgt ggccctgttg ttcatgattc cggccgacag cccggatcac
     1441 cggaaggaat ttgaagtatt gctgaatgaa ctcgaacaat ttaatccgga gctgctgcac
     1501 aaacgtttcg tgatcgccat cagtaaaagc gacctcctgg atgaagaatt aattaccgcg
     1561 gttgaaaagg aattaccggc cggcattccg catatcttca tttcctccgt tacccaaaaa
     1621 ggaatccagg cgctgaaaga cctgctatgg accataatga atgaagacaa atagctgtat
     1681 tatatttgaa aaacgaactt catgaaatgc atcgctctga tcccggccag gtatgcttca
     1741 acgcggttcc cggccaaact catgcaggta ctcggcagta aaacggtcat tcgacatacc
     1801 tacgacaata ccatagccac cgctcttttt gatgaggtga tggtggtcac cgacagtgat
     1861 ctcatttatc aggaaattac atcccatggc gggaaggcaa agatgagccg caaaacccat
     1921 gaaagcggta gtgaccggat agcggaggcc gtggaagata tggatgcgga tattgtgttg
     1981 aacgtgcagg gagatgagcc cttcgttcag aaaaagccct tagagaaact
//
LOCUS       DASYCU010000468         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_244891, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000468 DASYCU010000000
VERSION     DASYCU010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244891"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGM24_09445"
     CDS             <1..182
                     /locus_tag="VGM24_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY4155643.1"
                     /translation="DFAYYSQVTDSCFYFLGTRNSGKGIDSFLHTPAFNIDENALLIS
                     TGLMAYIAVKYLENS"
     gene            356..1978
                     /locus_tag="VGM24_09450"
     CDS             356..1978
                     /locus_tag="VGM24_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155644.1"
                     /translation="MKKFLLIGLACFMCGFLPAQGGSNRYTEFPDSVATHKVVLDKQS
                     KIISWISPQSVAYDHFLRDRWNFIFRGIPDCPGPPPRSNYPQYYFYCAYKNQKMIPDT
                     WMNDVGEKIPNWFESARLFYQYTGDRRALDIITNMAEYAIDHGSSPSGFAWPNFPYTT
                     TNAGDTLFRGFTNSKKLVLHEVQVDHAGDMGFTYYKLYLYTGNEKFKTAAIHVADALA
                     AHARVGTASQSVWPYRVVMSDGRITAQYGANWLGCYSLLDNLVRAKLGNVAAYRAAMV
                     KARNFILQFPMKTGYWTDGHTDTDVNSDTYKSNMSASNATLYIFDHPAFDPQYKTDIP
                     MLIQWTEKNFVFHSLPGEPSTQWGANIVGEQDGFLPKMDYQTARYGAECARWYSLSGD
                     TSYKEKAYRALNWVTYCSDSNGMAFESPLSKGVTSWWSDCYGEGPRMFYHALAAVPEW
                     APPGENHILYAEDVLKNVVYSGKKVEYQSSAPSGTEFLRLSFKPSAVRVNGTAISGAD
                     HLSKEGYTVKPLGGGDYSLVIRRSRAGQVTIE"
     gene            2021..2791
                     /locus_tag="VGM24_09455"
     CDS             2021..2791
                     /locus_tag="VGM24_09455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4155645.1"
                     /translation="MKFSNKCVVVTGSGSGIGRATALCFANEGADVVVADINLDAALE
                     TVELIGKANGKAIACKADVSDPSSVKDLVGYTIARFSRVHAIVNNAAIQVNKTAEDTS
                     FEEWNRQLAINLGGVFLCSKYFLPHLREAKGSIVNMSSVNGFFVEPTCAGYCATKAAI
                     IGLTKAMAIDHGHQGVRVNCICPGYIDAGLAEGYFQSQPDPARARLEAGKLHALWRIG
                     RPEEVGKVAVFLAGEDASFITGAAIVVDGGFGSGLPPK"
     gene            2849..>3610
                     /locus_tag="VGM24_09460"
     CDS             2849..>3610
                     /locus_tag="VGM24_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY4155646.1"
                     /translation="MRFVLPSYLSLWKGYKRKLALQVLFSFLISAAFAQEKMNVSGKV
                     TNGKTALSNVSVKVLGSKTGTTSDDQGNFRISVAKGQYLLFSYVGYAEQQVLVEDQST
                     VNVEMVISSLAFDSVVVVGYGTQKKSSLTAAISTMKGKEIASTPVSNLSNSLGGRVSG
                     VIVTQNTGDPGRDGSSIFIRGVSSTGSTQPLVIVDGVPRDFTQLDPNTIETFTILKDA
                     AAVAPYGVAGANGVILVTTKRGKRGDPTLTYNGYVG"
BASE COUNT          923 a          869 c          944 g          874 t
ORIGIN      
        1 aagattttgc ctattattcg caggtgaccg attcctgttt ttacttttta gggacccgca
       61 acagcgggaa agggatcgat tcctttcttc atacgccggc ttttaatatt gatgaaaatg
      121 cccttcttat cagcaccgga ctgatggctt atatcgccgt taaatattta gagaattcat
      181 aagtcaacgg ctgattttta caaaataggg taataagtaa tccggcatcg ggtagaaaat
      241 gatctcccgc gcccgttaaa tttgagttgt tattttacct gaccgctttt aacgtaaccc
      301 aattgcttgt ggtgagaatg cccatctcac taaccgcaat cgaaagaaac gaaacatgaa
      361 aaaattcctg cttataggtc ttgcctgttt tatgtgtggc tttcttcctg cgcaaggcgg
      421 ctccaaccgg tatacggaat ttcccgattc ggtggccacg cataaagtgg tgctggacaa
      481 gcagtctaaa attatctcct ggatctctcc ccaatcggta gcgtacgacc attttctccg
      541 ggatcgctgg aattttattt tccgggggat cccggattgc cccgggccgc cgccccgatc
      601 aaattatccg cagtattatt tttactgtgc ctataaaaat caaaaaatga ttccggatac
      661 ctggatgaat gacgtcgggg aaaagattcc gaactggttc gaatccgccc ggttattcta
      721 tcagtacacc ggagaccgga gagcgctgga tatcattacc aatatggcgg aatatgccat
      781 tgaccacgga agcagccctt ccggttttgc gtggcctaat tttccctata ccaccaccaa
      841 tgcgggagac acccttttcc gcgggttcac gaattccaaa aagctggtcc ttcatgaagt
      901 gcaggtggat cacgcgggag acatgggatt cacctattac aagctttacc tctatacggg
      961 gaatgaaaaa ttcaaaaccg ccgccatcca tgtggcggat gcgctggccg ctcacgcgcg
     1021 ggttggcacg gcctcccaat ccgtttggcc ttaccgcgtc gtgatgagtg atggaagaat
     1081 taccgcgcaa tacggggcaa actggctggg ttgctattcc ctgctggata atctggtccg
     1141 ggcaaagctg ggcaatgtag cggcctaccg ggctgcgatg gtaaaagcca ggaatttcat
     1201 cctgcagttt ccgatgaaaa ccggatactg gacggacggc cataccgata ccgatgtcaa
     1261 cagcgacaca tacaaaagca atatgagtgc gagcaatgcg accctgtata ttttcgatca
     1321 cccggcattt gatccgcagt acaaaacgga cattcccatg ttaattcaat ggacagagaa
     1381 gaattttgtg tttcattcac tgcccggcga accttccacc cagtggggtg cgaatatcgt
     1441 cggggagcag gatgggtttc ttcccaaaat ggattaccag acggctcgtt acggggcgga
     1501 atgcgcgaga tggtacagcc tttcgggaga tacttcgtac aaggaaaaag cgtaccgtgc
     1561 attaaactgg gtcacctatt gcagcgattc caatgggatg gcttttgaaa gccccctgag
     1621 caaaggagtg acgagctggt ggtcggattg ttacggagaa ggtccccgca tgttttatca
     1681 tgcgctcgcg gccgtacccg aatgggctcc gcccggtgaa aaccatattt tatatgctga
     1741 agatgtttta aagaacgtgg tttattccgg gaagaaagtg gaatatcaat cctcagctcc
     1801 ttccggtacg gaattcttga gactttcttt caagccatct gccgtgcggg tgaatggaac
     1861 ggccatttcc ggagcggatc atttgtcgaa agaagggtac acggtaaaac ccctgggtgg
     1921 cggagattat tcgctggtga tcagaaggag cagggcgggt caggtgacca ttgaataaca
     1981 ggaagaaaaa ggcctgcaat aattatttta aaagaagaaa atgaaattca gtaacaaatg
     2041 tgttgtcgtt accggttccg gatccggtat cggaagagcc accgccttat gctttgccaa
     2101 tgaaggagcg gatgtggtgg ttgccgatat aaacctggat gctgcactgg aaaccgtcga
     2161 gctgatcggg aaggcaaacg gaaaagcgat tgcgtgcaag gcggacgttt ccgatccttc
     2221 ttcggtgaaa gacctggtgg gttataccat tgcccgtttt tcccgggtcc atgcgatcgt
     2281 caataacgcg gcgatccagg ttaataaaac cgcggaggat accagctttg aagaatggaa
     2341 ccggcaactg gccattaatc tgggcggcgt tttcttatgc tctaaatatt ttcttccaca
     2401 cctgagggaa gcaaaaggca gtattgtaaa tatgtcttcg gtaaacggtt tttttgtcga
     2461 acctacctgc gcagggtatt gcgccacgaa agccgcgatc atcggactca ccaaggcgat
     2521 ggccattgat cacggtcatc agggggtgcg ggtgaattgt atttgcccgg gctatatcga
     2581 tgccggcctc gccgaaggct attttcagag ccagccggac ccggcccgcg cccggctgga
     2641 agccggaaag ctgcatgcct tgtggcgaat cggaagaccc gaggaggtcg gaaaagtagc
     2701 cgtcttcctg gccggtgaag atgcctcctt catcaccggt gcggcgatcg tcgtagacgg
     2761 cggattcgga tcgggattgc ctcccaaata atccggaata acatttatcg aattaaattt
     2821 tactaattgt aatattaaaa ctattgctat gagatttgta ttgccctcat acctgagcct
     2881 atggaaagga tacaaaagaa aactggcact ccaggtcctg ttcagctttt taatttctgc
     2941 cgccttcgca caggagaaaa tgaacgtttc cggtaaagtg acgaacggaa aaacagcctt
     3001 gtcaaatgtt tccgtcaagg tgcttggcag caaaacggga accacctcgg atgaccaggg
     3061 aaatttcagg atctccgtcg ccaaaggaca atatctttta ttttcctatg tcggatacgc
     3121 cgaacaacag gtgttggtgg aagatcaatc gacggtcaat gtggaaatgg tgatcagttc
     3181 actggcattc gactcggtgg tggtggtcgg ttacggcacg caaaagaaat catccctgac
     3241 cgccgctatt tccaccatga aaggaaaaga gatcgcctcc acgccggtct cgaatttaag
     3301 caacagcctg ggcggccgcg tttccggagt catcgtaacc cagaacaccg gcgatccggg
     3361 ccgcgacgga tcaagcattt ttatccgggg agtttcttca acgggatcca cccagcccct
     3421 ggtgatcgtg gatggcgttc ccagggattt cacgcagctt gatcccaata cgatcgagac
     3481 gttcacaata ctgaaagacg ccgcagcggt ggctccctat ggagtggccg gcgccaacgg
     3541 cgttatcctg gtgacgacaa aaagaggaaa aaggggagac cccacactta cctataacgg
     3601 ctatgtcgga
//
LOCUS       DASYCU010000469         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4450666, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000469 DASYCU010000000
VERSION     DASYCU010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4450666"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1002)
                     /locus_tag="VGM24_09465"
     CDS             complement(7..1002)
                     /locus_tag="VGM24_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY4155647.1"
                     /translation="MNKIIALVCCIFLFSNYSPAQTAGGYKVSKTFKIKSSGGWDYIA
                     VNNHRLYVSHGSQVNILDERTGDSVGVIPHTDGVHGIAFINRLGKGYTSNGRLNNVTV
                     FDLKTNAVLKQISTGENPDAIFYDPYSRKIITCNGRSHDLSVIDPDQEKVTMTIPLSG
                     KPETAVSDEAGKIYVNIEDKGAISQVDITSGKVLNTWSLAPAEGPTGLVIDRKTKRLF
                     AGCEKMLVVMNAGTGEVTARLPIGEGCDGVAFDPSSKNVFASCGEGNLYLAAEDSKGD
                     CKIIGSVPSKRSARTITIDTENHEVFLPAADLGKGEPGKRPPVIPGSFQVLVFAK"
     gene            complement(1029..4082)
                     /locus_tag="VGM24_09470"
     CDS             complement(1029..4082)
                     /locus_tag="VGM24_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY4155648.1"
                     /translation="MKNYFTFNKNPIIACLCIILLGGLFVYGQLKTSLFPEITFPKIK
                     IIADAGLQPVDKMMVTVTKPLENAIKQVPDLVNIRSTTSRGSCEISAFMDWNTDIDID
                     QQRIESRIAQVTNTLPPGVQISVEKMNPSILPVMGYSLETKDYRAPIDLKMLALFTIK
                     PFLSQVPGVSEVRVTGGKQKEYWIRLNRSKMSTLGVTPDMITTAMAQTNFIKSNGYMA
                     DYHLLYLTVTDATVKSISQIQSIVVSNTGKRVIRLKDFADIAVKEAIEYTKINANGRE
                     SILISVVKQPNANLIDLSAQMESKVAEMRKILPAGMDLKPYYVQADFVNDSVRSVKDS
                     LWIGLLLAIFVSILFLRSLKASATILFTIPVTIALSILCIYGVGYTFNIMTLGAIAAS
                     IGLIIDDAIVVVEQIQRTHEEYPEEPSSVLVQKAIRYLFPAMVGSSISTIVIFIPFEW
                     MSGVAGAYFKVLTNTMILTLVCSFFVTWIGLPVIYLLLTGKNKNLLRKKRKPEKVKEH
                     QWISVFIHRPWISFILVAAMITSIYLVLPRLQTGFLPEMDEGSIVLDYTSPPGTSLEE
                     TDRMLRQVEKLIVRVPEVASYSRRTGTQMGFFITEPNTGDYLIQLRKDRSRSTEDVIT
                     DIRQKIESSQPALRIDFGQVIGDMLGDLMTSVQPVAIKIFGNDSKTLESLSRQVAGIV
                     GKVQGTADVFDGIVIAGPSVTVEPDYEKIAQFGITPASLQYQMQTGLEGNIVGTVQEK
                     EQMTPIRLIDADSSHRSTEGLRHLQIFLPSGKLKPIAELATVRVNNGDAELKRENLQA
                     MGEVTARLENRDLGTVMRDIRQKVGSEIALPQGYHIEYGGAYADQQQSFKELLMILIA
                     SSLMVFAVILFLFKDFRVAFLIIGLAVLGISGSYLALYLTGTPLNVGSYTGLIMIVGI
                     IGENAIFTFLQFRDSYKEGTVDHSIVYAISTRLRPKLMTALGAIIALMPLALGIGAGA
                     QLHQPLAIAVIGGFIVALPILLIILPSMLRLVYKKHKDPSEFRQI"
BASE COUNT         1007 a         1026 c         1004 g         1087 t
ORIGIN      
        1 agcctgctat tttgcaaaca ccagcacctg gaaactgccg ggaataacgg gcggacgttt
       61 tccgggttca ccctttccga gatccgccgc cggaaggaaa acttcatgat tctctgtatc
      121 gatcgtgatg gttctcgcgg atcttttcga aggcaccgac ccgatgatct tgcaatcgcc
      181 tttgctgtct tcggcagcaa ggtacaggtt gccttccccg caggaagcaa aaacattttt
      241 gcttgacgga tcaaaggcca cgccgtcaca gccttcaccg atcggaagcc ttgccgtaac
      301 ctcgccggtg ccggcattca tgaccacgag catcttttca cagcctgcga agagtctttt
      361 tgtcttccgg tcaataacca gcccggtcgg cccttcggcg ggcgcaaggg accaggtatt
      421 cagcactttc ccgctcgtaa tatcaacctg gctgatggca cccttatctt ctatgttcac
      481 atagatctta cctgcttcat cggatacggc tgtttccggc tttccggaca ggggaatggt
      541 catggtcact ttctcctggt ccggatcgat cacgctcagg tcatgacttc gaccattgca
      601 ggtaataatt ttcctggagt acgggtcata aaaaatggca tccggattct cgcccgtgga
      661 gatttgtttg agcacggcat tcgttttcag gtcaaaaacg gtaacgttat ttaatctacc
      721 gttgctggta tatcctttcc caagccggtt tataaaggca atgccgtgca cgccgtccgt
      781 atgggggata accccgacgg aatcgccggt ccgctcatcc agtatattta cctggctgcc
      841 gtgggaaacg taaagccggt ggttatttac tgcaatataa tcccagccgc cgctgctttt
      901 tattttaaag gttttggaga ccttgtagcc cccggccgtt tgggcagggg aataattact
      961 gaagagaaaa atacagcata cgagagcgat gattttgttc atttgcagga tttgccggaa
     1021 tttaaaactt aaatctgtcg aaattctgaa ggatctttgt gctttttgta aacgaggcgc
     1081 agcatggatg gaaggatgat cagcagaatg ggaagcgcta ctataaatcc accgatgact
     1141 gcaatggcca gcggctggtg caactgggca ccggctccaa tacccagggc cagcggcatc
     1201 aatgcgatga tggcgcccag cgccgtcatt aacttgggcc tcaggcgggt tgaaatggca
     1261 tatacgatcg agtggtctac ggtgccttcc ttataagaat cccggaattg caaaaaagta
     1321 aatatggcgt tttccccgat gattcccacg atcatgatca gtccggtata gctgcctacg
     1381 ttcaggggcg taccggtaag gtatagcgcc aggtagctgc cggatattcc cagcacggca
     1441 aggccgatga tcagaaaggc gacgcgaaaa tctttgaaga gaaagaggat gacggcaaac
     1501 accatcaggc tggatgcaat caggatcatc aataattctt taaacgactg ttgctgatct
     1561 gcataggctc ccccgtattc gatatgatac ccttgcggaa gtgcgatttc agatccgacc
     1621 ttttgccgga tatcccgcat aacagttccc agatcgcggt tttccaggcg cgccgttacc
     1681 tctcccatgg cttgcaggtt ttctcttttc agttctgcat cgccgttgtt cacgcgaacg
     1741 gtggctagtt ccgcgatggg tttcaatttg cccgagggca gaaagatctg caaatgccgg
     1801 agcccttcgg ttgaccggtg gctgctgtct gcatcaatca accggatggg agtcatttgt
     1861 tctttttcct gcaccgtgcc gacgatattt ccttccaggc ccgtctgcat ctggtattgc
     1921 agcgatgcag gcgtaatgcc aaactgggcg atcttttcat aatccggttc taccgttacg
     1981 gaaggtcccg caatgacaat gccgtcaaat acatcagccg taccctgtac tttgcccact
     2041 atcccggcaa cctgtcgcga aagggattca agcgttttgc tgtcgttacc aaaaattttg
     2101 atggcgactg gttgcaccga ggtcatcagg tccccgagca tgtctccgat cacctgacca
     2161 aaatcaatcc gtaaagccgg ttggctgctc tcgatctttt ggcgtatatc cgtaatcacg
     2221 tcttccgttg accggctgcg gtctttcctc aactgaatga gatagtcccc cgtattgggt
     2281 tcggtgatga aaaatcccat ctgggttccg gttctcctgg aataacttgc cacttccgga
     2341 acacgaacga tcaatttttc gacctgccgg agcatccggt ccgtttcttc cagcgaggtt
     2401 cccggcgggg aagtataatc caaaacgatg ctgccttcat ccatttccgg taaaaatccg
     2461 gtctgcaggc gaggcaatac caggtaaatg gaagtaatca tggccgctac caggataaaa
     2521 ctgatccagg gcctgtgaat gaaaacggaa atccattggt gttcttttac tttttccggc
     2581 ttacgttttt tccttaacag gtttttgttc tttcccgtca gcaaaagata tattaccggc
     2641 agcccgatcc aggtaacaaa aaaagaacag accagggtca ggatcatggt attggtcagc
     2701 actttgaaat aagcgcccgc aaccccgctc atccattcaa aaggaataaa aataacgatg
     2761 gtgctgatgg aagaaccgac catcgcagga aacaaataac gaatcgcttt ttgaacgagc
     2821 acggaggaag gctcttccgg atactcttca tgcgttcgct ggatttgttc caccacaacg
     2881 atggcatcat cgatgatcag acctatggat gccgcgatgg cgcccagcgt cataatattg
     2941 aacgtataac cgacgccata aatacagaga atggataggg cgatggtgac gggtatcgta
     3001 aaaagaatcg tcgcgctggc tttaagggag cgaagaaaaa gaatggaaac aaaaatcgcc
     3061 agtaacaagc cgatccacag actgtctttt acacttctga cggaatcgtt tacaaagtcc
     3121 gcctgcacat aatagggctt taaatccatc ccggcaggaa gtatctttcg catttcggct
     3181 actttgcttt ccatttgggc agacaggtcg atcaggtttg cattgggctg cttcacaacc
     3241 gatattaaaa tactctccct gccattcgca ttgatttttg tgtactcgat ggcctctttg
     3301 acggcgatat cggcaaaatc tttcagccgg ataactcttt tcccggtatt gctgacgacg
     3361 atggattgaa tctggcttat gcttttaacg gtggcatctg ttacggtaag atataaaaga
     3421 tggtagtccg ccatgtagcc gttagacttt ataaaattgg tctgggccat ggcggttgta
     3481 atcatatccg gcgttacccc gagggtactc attttggacc tgttcaaacg tatccagtat
     3541 tccttttgct ttccgccggt cacgcgcact tccgacacac cgggaacctg ggaaagaaat
     3601 ggtttgatcg taaacagggc cagcattttc aggtcgatgg gagcgcggta atcttttgtt
     3661 tccaatgaat accccatcac cggcagaata gaagggttca ttttctcgac actgatctga
     3721 acgccgggcg gaagcgtgtt ggtaacctgg gctattctgg attcaatccg ctgctggtca
     3781 atatcgatat cggtattcca atccatgaag gcggaaattt cgcagcttcc ccggctggtt
     3841 gtgctacgga tattcaccag atcaggtacc tgttttatgg cattctccaa tggtttggta
     3901 accgttacca tcatcttatc gaccggctga agaccggcat cggcgatgat ttttattttt
     3961 ggaaaagtga tttccgggaa aagagaagtc ttgagctgac catagacaaa taagccaccc
     4021 aataaaataa tacagaggca ggcgattatc gggttcttat tgaaagtaaa ataattcttc
     4081 atggcattat ttctctatca ctactttggc ggtatccggt aatc
//
LOCUS       DASYCU010000470         6022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2418906, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000470 DASYCU010000000
VERSION     DASYCU010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2418906"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..887)
                     /locus_tag="VGM24_09475"
     CDS             complement(24..887)
                     /locus_tag="VGM24_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155649.1"
                     /translation="MEKRKNYNTISPSARSILLLKGCTNIPFAREVAGLLTHPGPFLP
                     AGENRDFSFWARVVHFENRYWSIDQLLEGNETKNILELSSGYSFRGLDRVARNPVHYI
                     DTDLEDVISLKRKFISVLEKKLPSINGSLEILPLNALDEMQFKEIVRRFEDGPLVIVN
                     EGLLMYLDLEEKKKLCALIHQELKARGGIWITADIYIRRPPSGAVIEQSEAEKYFYGQ
                     HRIEENKFESFEAAESFFKNAGLKKLKTARPDHGKLSTLPHLIGTASAEQEKKLQAAG
                     KIQETWMLEAE"
     gene            1023..2585
                     /locus_tag="VGM24_09480"
     CDS             1023..2585
                     /locus_tag="VGM24_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155650.1"
                     /translation="MARKPSYYLLLIILFPGLLPAQRVSYSSYLNLRSPNTFQVIGKS
                     GDYYWIEKEQVRKSFTRHESGSVLESQRFAAYDSRLNFVNEIPALNIPGTIKQYLVAG
                     RAAFDQLILSAAPGKTKIFINRYQAAEIPESSSRLIDSFSFPASGSRFLLARSEDLSK
                     IILIGFESGASSLRLHTILFDQDWKVLYHTVLEHPYFTQPCIQDDYISFPAESFDNLP
                     VKLANNGDWLMASSSRTNRNYLLFHFYDDGKRYYFREIPLSSYYNMEDIAMSIDNEHQ
                     EMSVGLLSGYRNTSLKNVEVTHYSMTRTKFDFDSSYHFNSLAGGLKNQNLSNACFISV
                     PGEGYMLFKEYGRPEDPRDLQPPDVQPGDPVLLMASFSGNEEQSTRLDNGYSFRKGLQ
                     GIRSVFNRGNLTMFYFPIKRTDSTWSGIINTEQTTELNSPSLSYLVFPVKNKVYILYN
                     SQIRNDDEFSTTTALNVHGQATDDALIFWKMNRTLNFQQSRRISLDEVAVPFRVNQQN
                     GFAIIRLMADAK"
     gene            2639..3112
                     /locus_tag="VGM24_09485"
     CDS             2639..3112
                     /locus_tag="VGM24_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4155651.1"
                     /translation="MSETNITEKTNDQPRWLTIFRVALGIILLLKGISFIRDTATLKS
                     MIQQTGLETFARHSDIWAFLVAFINLLGGLFIACGLFTRTSSIVQIPILIIATFFVNI
                     RPAAGSSLELILSIVVLGLLILFAIKGSGALSADEYFRSYYKAGDEEGNTRKFFK"
     gene            complement(3143..3685)
                     /locus_tag="VGM24_09490"
     CDS             complement(3143..3685)
                     /locus_tag="VGM24_09490"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY4155652.1"
                     /translation="MKALPMSVVKLMVTTSFFAGLFLSCHAQHKPLAVGDTVPAFSLK
                     DQNDSLFNISDYVGKHILVIYFYPKDESRVCTKEACSFRDGYADFTNGGAMVVGINSG
                     SVESHKHFQLNHQLPFTLLSDPGNKVLKLFGVKSKFLISGRETFVIDKSGKVRFTYDS
                     FSNGPAHEAEALRFIKTLRG"
     gene            complement(3712..4134)
                     /locus_tag="VGM24_09495"
     CDS             complement(3712..4134)
                     /locus_tag="VGM24_09495"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY4155653.1"
                     /translation="MKRTAKAHWNGTLKEGKGELSTQSTTLNKTQYSFKTRFEQGVGT
                     NPEELIAAAHAGCFTMAVGAALTQAGFTPGDLDTEATLDLDMAALKITGIQLDLKAAS
                     IAGVSEAKFKEIAEGAKANCIISKALSVPITLSVSYQG"
     gene            4304..4738
                     /locus_tag="VGM24_09500"
     CDS             4304..4738
                     /locus_tag="VGM24_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY4155654.1"
                     /translation="MFSKTCEYAIRAMIFIAQKSKNGYKTGIKEIARGIDSPEYFIAK
                     ILQNLSRRGLVQSLKGPAGGFYLDKQSLKYSLADIVKAVDGGRLFSGCALGLDYCSEA
                     KPCPIHHEFKKVRKELEDILQEARLGTFYEQFEKNLAFLKQR"
     gene            4749..5144
                     /locus_tag="VGM24_09505"
     CDS             4749..5144
                     /locus_tag="VGM24_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEY4155655.1"
                     /translation="MPEKKDIQNLDDIKKLVDAFYEKVRVDPKLAPIFNERIRDHWPA
                     HLERMYRFWETVLLDGHSYLGSPFPPHASLPVDQTHFEQWIKLFRETLDEYFSGKKAD
                     EARWRADKMAEMFLLKINYSRERGFKNIM"
     gene            5176..5508
                     /locus_tag="VGM24_09510"
     CDS             5176..5508
                     /locus_tag="VGM24_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY4155656.1"
                     /translation="MNGSGIHLTVMYEGNWYELDTVEGEYRNLMMLIFDRIYTEEFGD
                     CKGMGRCGTCLVEILSEDHCLGSMARNEESTIRKITAPRKDIRLACQLLINSRLHNIK
                     IKILRDGQ"
     gene            complement(5502..>6022)
                     /locus_tag="VGM24_09515"
     CDS             complement(5502..>6022)
                     /locus_tag="VGM24_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4155657.1"
                     /translation="EVEDVMQVAYIKAYENLRKFAFKSSFSTWLTRIFINESLLRLKK
                     RKLSINLNDLMMKNELSSAHSLEPQTPASKALNAELKTILEEAIRQLPEIYRTVFVMR
                     EIENMNVLETKNCLDISEANVKVRLNRAKSLLRTSLNRYYKKEDILHFHLSRCDRMVE
                     NVSRQLESIPIY"
BASE COUNT         1516 a         1491 c         1410 g         1605 t
ORIGIN      
        1 ccaggagtca tccgctgtcc actttattcc gcctccagca tccaggtctc ctgtatcttt
       61 ccggctgcct gcaatttttt ctcctgttcc gctgaagccg ttccgatcaa atgaggcaga
      121 gtgcttagtt taccatggtc cggcctggcc gttttcagct ttttgagccc ggcattttta
      181 aagaaagatt cagccgcttc aaaactttca aatttatttt cttctatccg gtgctgccca
      241 taaaaatatt tttccgcctc gctctgctca atgacagcac cggatggcgg ccttctgata
      301 taaatatccg ccgtgatcca aatgcccccg cgcgctttca gttcctgatg aatgagcgca
      361 cagagttttt tcttttcctc taaatccagg tacatgagca gtccttcgtt gacaataacg
      421 agtggtccgt cttcaaaccg gcggacaatc tctttgaatt gcatttcatc cagcgcgttc
      481 agcggaagaa tttccagtga tccgtttatg gagggaagtt ttttttccag gaccgaaata
      541 aatttccgtt tcagcgagat aacgtcttcc aggtcggtat caatataatg aacggggttt
      601 cgggcaaccc ggtccagccc ccggaaagaa tagcccgatg acaattctag tatgtttttc
      661 gtttcatttc cctccagaag ctgatctata ctccaatacc ggttttcaaa atgaaccacc
      721 cgggcccaga aagaaaaatc cctgttttct cctgccggaa gaaagggccc ggggtgggtc
      781 agcaggcccg ccacttccct ggcaaacggg atattggtgc accccttcag caataaaatg
      841 gatctggcag aaggactgat ggtattgtaa ttcttccttt tctccatatt cccaaattac
      901 tgattaatcg gaagcccggg tcaaaaccgg tttgctccgc tggcagatat taatttaccg
      961 gccggttcgc tacaatgggg gttcgcattc aaaataatgc taacttatgg tgcttaacca
     1021 ccgtggccag aaaaccatca tattaccttc tcctgataat attgtttccg ggacttttac
     1081 cggcccagcg ggtcagctat tcttcctacc tgaacctccg ctctcccaat acgttccagg
     1141 taattggcaa atccggcgac tattactgga tcgaaaagga acaggtccgc aaatcattca
     1201 cccgccatga atccgggtcc gttttggaat cacagcgttt tgccgcttac gacagccgct
     1261 tgaattttgt gaatgaaatt cctgccctga atattccggg aacgatcaag caatacctgg
     1321 tcgccggaag agcggctttc gatcaattga tcctgtccgc ggcgccggga aaaaccaaaa
     1381 tttttattaa ccggtaccag gcagcggaaa taccggaaag cagcagccgt ttaatagaca
     1441 gcttttcatt cccggcatcc ggaagccgtt tcttgctggc gcggtcggaa gatctctcga
     1501 aaattatttt gattggtttt gaatccggcg cctcctccct ccggttgcat accattttgt
     1561 ttgatcagga ttggaaggtt ctctatcata ccgtactgga gcatccttat tttacccaac
     1621 cttgtatcca ggacgattat atttcatttc ccgcggagag tttcgacaac ctgcccgtta
     1681 agctggccaa caacggggac tggctgatgg cttcttcctc caggaccaac cggaactacc
     1741 tgctttttca tttttacgac gacggcaaaa gatattactt ccgggagatc ccgctttctt
     1801 cctattataa tatggaagat attgccatgt ccatcgacaa tgagcaccag gaaatgagcg
     1861 tcggcctcct gtcgggttac cggaatactt ctttgaaaaa tgttgaagtc acgcattatt
     1921 ctatgacgcg aacaaaattc gactttgatt catcctatca tttcaactcg ctggccggcg
     1981 gcctcaaaaa tcagaacctc agcaacgctt gttttatctc tgttccggga gaaggatata
     2041 tgttgtttaa agaatacgga agacccgaag atccccggga tctgcagcca ccggatgtgc
     2101 aaccggggga tccggtttta ttgatggcca gtttttccgg caatgaagaa cagtcgaccc
     2161 ggctcgataa cgggtattcc ttcagaaagg gtctgcaggg gatacgctcc gtttttaacc
     2221 ggggcaacct gacgatgttt tattttccca ttaaacggac agacagcacc tggagcggga
     2281 tcatcaatac ggaacagacc actgaactga attccccttc cctttcctac ctggtattcc
     2341 cggtgaaaaa taaagtctac attctttata acagccagat ccgcaatgat gatgagttca
     2401 gcaccacgac cgcattgaac gttcatggcc aggcgaccga cgatgcgctg atcttctgga
     2461 aaatgaacag gaccctgaac tttcagcaat cccgccggat atcacttgat gaagtggccg
     2521 tccctttccg ggttaaccag caaaacgggt ttgccattat ccggttgatg gcggatgcaa
     2581 aatagcggaa ggcatctccg gggtgaacca ggaacaaaat ttattaatac gcaagatcat
     2641 gtccgaaaca aacatcaccg aaaagaccaa tgaccagccc cgctggctga ccatattccg
     2701 ggttgccctg ggaattattc tgctgctgaa gggaatcagc tttatccggg acacggccac
     2761 cctgaaatcg atgattcagc aaaccggcct ggagaccttt gcccggcatt cagacatctg
     2821 ggccttcctg gttgctttta tcaacctgct ggggggatta tttattgcct gcggactttt
     2881 taccaggact tcttccatcg ttcagattcc gatcctcatc atcgccacct tttttgtaaa
     2941 tatcaggccg gccgccggga gcagtctcga actgatcctt tccatcgttg ttctgggcct
     3001 gctcatcctt tttgcgatca aaggaagcgg cgctctttcc gcggatgaat atttccgcag
     3061 ttattataag gccggcgatg aagaaggcaa taccaggaag tttttcaaat agccggaacc
     3121 cgaggccata accggtcgcg ggtcagcccc tgagcgtttt gataaaccgg agcgcttccg
     3181 cctcatgcgc cggtccgttg ctgaacgaat cgtaggtgaa gcggaccttc ccggatttat
     3241 caatcacaaa cgtttcccgg ccggaaatca aaaatttact cttaacgcca aataatttca
     3301 ggaccttatt tcccggatca ctgagcaggg tgaagggcaa ttgatgattc agctgaaaat
     3361 gtttatggct ttccaccgaa ccggaattga ttccgaccac catcgctccc ccatttgtga
     3421 aatctgcata tccgtcccgg aaagaacagg cttctttggt gcaaacccgg ctttcatcct
     3481 taggatagaa atagatcacc aggatgtgtt tcccgacata atccgatata ttaaacaggc
     3541 tgtcattctg gtccttcagg gaaaatgccg gcacggtatc tcccacggca agcggtttat
     3601 gctgcgcatg gcaggacagg aacaggccgg caaaaaatga agtggtgacc ataagcttaa
     3661 cgacagacat agggagggcc ttcatttgtc cgcatatctt tttgtttgga attacccttg
     3721 gtaactaaca cttagagtga tcggcacgct caatgctttg gaaataatac aatttgcttt
     3781 tgctccttcc gcgatctctt tgaatttggc ttcgctcacc cccgcaatgg aagccgcttt
     3841 cagatccagc tgaattccgg tgatcttcag ggctgccata tccagatcaa gcgttgcctc
     3901 cgtgtccagg tcgcccgggg tgaaaccggc ctgggtgagg gctgccccta cggccatggt
     3961 gaaacaaccg gcatgcgctg cagcgatcag ttcttccgga ttggtaccca cgccctgttc
     4021 aaaacgggtc ttgaatgaat actgcgtctt gttcagggtg gtgctctggg tggaaagttc
     4081 cccctttccc tctttcaggg tgccgttcca atgtgccttt gctgtacgtt tcatgatgtt
     4141 gtattttcag gatttacaat tcaggcaatc ttttgttcaa atcattctcc ttccggggaa
     4201 aatttatttt ttaatacagt cgaagtcgga tcccctttaa aataaaaaag gacaaaaaag
     4261 tcttttatta acccttttga ttatttttgc actaaaataa agcatgtttt caaagacctg
     4321 cgaatatgcg atccgggcaa tgatcttcat tgcgcagaag tcgaaaaacg gatataaaac
     4381 gggcatcaag gaaattgccc gggggatcga ttctccggag tattttattg caaagatcct
     4441 tcagaacctg agccgccgcg gcctggtgca atccctgaag ggccctgccg gcggttttta
     4501 cctggataaa caatccctca aatattcact ggcggatatt gtaaaagccg ttgacggagg
     4561 caggcttttt tccggatgcg cgctgggact ggattattgt tcggaagcaa agccctgccc
     4621 catccaccat gaatttaaaa aagtgagaaa ggagctggag gatatattac aggaagcccg
     4681 gctgggtact ttttacgaac aatttgaaaa aaacctggct tttctgaaac aacgttaaac
     4741 tgatttatat gccggagaaa aaagacatcc agaacctgga tgatatcaaa aagctggtgg
     4801 atgcatttta tgaaaaagtg agggtagatc cgaaactggc ccccattttt aatgaacgga
     4861 tccgggatca ctggcctgcc cacctggaaa gaatgtaccg gttctgggaa acggtcttac
     4921 tggacggcca ttcgtatttg ggaagcccct tccctcccca tgccagtctg cccgtggacc
     4981 agactcattt tgagcaatgg ataaaattat tcagggaaac gctggacgaa tacttttccg
     5041 gaaaaaaagc ggacgaagcc cggtggcgtg cggataagat ggcagaaatg ttcctgctca
     5101 agataaatta ttccagagag aggggattta aaaatattat gtaatgagga ttcgtctcaa
     5161 ataccccttt acggcatgaa cgggagcggg attcatctca cggtcatgta tgagggaaat
     5221 tggtatgagc tggatacggt ggaaggggaa taccggaatc tcatgatgct gatcttcgac
     5281 agaatatata cggaggagtt tggagattgc aaaggaatgg gccggtgcgg aacctgcctg
     5341 gtggagatcc tgtctgaaga tcattgcctg ggatccatgg caagaaatga agaatccacc
     5401 atccgcaaga tcacggcacc gcggaaggat atccggctgg cctgccagct cctgatcaat
     5461 tcccggctgc ataatatcaa aataaaaatt ttacgggacg gtcagtaaat aggtatggat
     5521 tcaagctgcc ggcttacgtt ctccaccatc cggtcacacc gcgaaagatg aaaatgcagg
     5581 atatcttctt tcttgtaata acggtttaaa gaggttctca atagggactt cgcccggttc
     5641 agccggacct tgacattggc ttcgctgatg tccaggcaat tttttgtttc cagcacgttc
     5701 atattttcaa tttcccgcat cacgaacacg gtccggtata tctccggaag ctggcggatc
     5761 gcttcttcca ggatggtttt taattccgca ttgagcgctt tggaggccgg ggtttgcggc
     5821 tctaaggaat gtgcggaaga taattcgttt ttcatcataa ggtcattcag gtttatggac
     5881 aattttcttt ttttcaaccg cagcaggctt tcgttaatga atatccgggt cagccaggtg
     5941 gaaaaactgg atttaaaggc gaacttccgc agattctcgt aggccttgat ataggccacc
     6001 tgcatgacgt cttccacttc cg
//
LOCUS       DASYCU010000471         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1985916, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000471 DASYCU010000000
VERSION     DASYCU010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985916"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="VGM24_09520"
     CDS             <1..1710
                     /locus_tag="VGM24_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4155658.1"
                     /translation="FISNFSFMIGKMYSLLVIFFLTFLSQSFAQDNTTENSSYDLRDS
                     SLIPSRRIPQHNEFLNNAYPFPAKPRNELEIGIKGGLSSVSGDVRSWLPTFGAGVHVR
                     KALGYVFSLRLEYDWLRAKGLNWQPSATGYGANPVLNPIYGGGATHQPVFYNYRTTIQ
                     ELSLQGIVTLNNIRFHKSKSGIAIYLLGGIGGMTYSTLYNLKDASGNYYNYSDIVTQF
                     GGGGFTYSERKDIKKALKSKLDGSFETLAERDKSQPSIFGGTPFRPVVVFGTGVEIKL
                     SNRFNIALEDKVSVSKSDLIDGQQWQETGSQAVHSMTRDYDSYNFLSVGLNYNFGNKA
                     VEPLWWINPLDYAYGEINAPRHMKLPKPVLDDADGDGVTDQFDHEPNTPAGCPVDTHG
                     VSRDTDGDGVPDCKDKELITPTQCQPVDADGVGKCPDPACCKEISARLDSGGVGSMGR
                     SRCSIGDLPSIVFKGRSVTLSKDGKALAASIADKMRENPACKVAVIGYGESSKSAQQL
                     SWDRVNEVIKYLVEKEGISSDRFIFRYGQTGGDENTIDIKDGTGEEGPNTVPAPHPNL
                     RKR"
     gene            1862..2725
                     /gene="bamD"
                     /locus_tag="VGM24_09525"
     CDS             1862..2725
                     /gene="bamD"
                     /locus_tag="VGM24_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY4155659.1"
                     /translation="MKPLFLAVVISVSLSSCFILPKAWRGTGQGFSKIMKSTDYDYKL
                     KMAEKYYLNKDYNHAQQLYDDLYRVMKGSEHYEDIWYKYAYSAYYLHDYINAENLFKG
                     FVEAFPNSPKAAEMDYMRAFCFYKQSPKYELDQTNTTKTIGFMQAFISQHPESDKVKD
                     ATGIIDLCRAKLEQKDFENARLYYNLRATNTAYLKAASLTFNTLMNTYPDSQKSDEYK
                     LYIIRADYEYAERSFEDKKQARFEQVVTDCNDFIDRFPDSKLKKQVQEYLDLTNNSLK
                     ALQNEQAQKTS"
     gene            2700..3041
                     /locus_tag="VGM24_09530"
     CDS             2700..3041
                     /locus_tag="VGM24_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY4155660.1"
                     /translation="MSRLRRQVSTHISSTAETRNLTDLKNKTGNLYESIAIIAKRANQ
                     INISMKEELHSKLEEFATHTDSLEEIHENKEQIEISKAYERMPNAALLATQEFMEDKI
                     YYRKNEDDLFS"
     gene            3058..4248
                     /gene="coaBC"
                     /locus_tag="VGM24_09535"
     CDS             3058..4248
                     /gene="coaBC"
                     /locus_tag="VGM24_09535"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY4155661.1"
                     /translation="MVKGKKILLGVCGSIAAYKSILLTRLLVRAGAEVKVILTPSAKD
                     FVTPLSLSTLSRNEVLSDLFREDNWSNHVELGRWADLLLIAPLSCNSLAKMANGHCDN
                     LLLAVWLSANCPVAFAPAMDEDMWLHPATRSNIEKLQSFGNYLVPAEKGELASGLYGE
                     GRMAEPENILSFIEKNFFFSRELNGKKALVTAGPTQESLDPVRYISNHSTGKMGIALA
                     RELALHGAEVELVIGPSALPVNFPAVKVVPVQSAEEMYQACLERFPGTDIAVLSAAVS
                     DYMPVSFSPEKIKKNAQTFSLELTRTKDILKTLGERKRTGQLLVGFALENRDEETNAR
                     KKLESKNADMIILNSLNDPGAGFGFDTNRVTIFEKSGPEIRYDQKSKQQVAKDIVDRI
                     IKLS"
     gene            4248..>4484
                     /locus_tag="VGM24_09540"
     CDS             4248..>4484
                     /locus_tag="VGM24_09540"
                     /inference="COORDINATES: protein motif:HMM:NF027445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HEY4155662.1"
                     /translation="MRTRILLWFVFSSLAFTSVRAQELKANITVLSNRIGSTVNKQVF
                     QTLQTALYEFLNNRKWTNEHFNDEEKIPCNFLLNL"
BASE COUNT         1211 a         1102 c         1095 g         1076 t
ORIGIN      
        1 ttcatatcaa acttcagttt tatgataggg aaaatgtact cgttgctagt gattttcttt
       61 ctaacctttc tctcacaatc tttcgcacag gacaatacga cagaaaacag cagttatgat
      121 ttgcgtgatt catccctgat tccttcccgg agaatccccc agcataatga atttctgaat
      181 aatgcttacc cttttccggc aaagccccgg aatgagctgg aaattgggat caagggtgga
      241 ttatcctccg tttccggcga tgtccggtcc tggctgccga cttttggtgc gggtgttcat
      301 gtaagaaaag cattgggtta tgttttctcc ttgaggctgg aatacgactg gctccgggca
      361 aaaggcctga actggcagcc ttccgctacc ggttatggcg ccaatcccgt tttgaatccg
      421 atttacggcg ggggagcaac ccatcagccg gttttttata attaccggac caccatccag
      481 gaactttctc tccagggtat cgtcacctta aacaatatcc gttttcacaa atccaaatcg
      541 ggcattgcga tttatttact cggcgggatc gggggcatga cctacagcac cctgtacaat
      601 ctgaaagatg cttcaggaaa ttattataac tactcggata tcgttaccca gttcggcggc
      661 ggcggcttta cctattctga aagaaaggat atcaagaagg cgctgaaaag caagctggac
      721 ggctcttttg agaccctggc cgaaagggat aaatcacagc cgagcatttt cgggggtact
      781 ccgttccgcc ccgtggtcgt atttggaaca ggtgttgaaa tcaaactttc caaccggttc
      841 aatattgctc tggaagacaa ggtatcggtt tccaaatccg atttaataga tggccagcaa
      901 tggcaggaaa ccggatcgca ggcggttcat tccatgaccc gcgattacga ttcctataat
      961 tttctttctg tcggactcaa ctacaacttt ggcaataagg cggttgaacc cctctggtgg
     1021 atcaatccgc tggattatgc ttacggcgag atcaatgctc cccggcacat gaagcttcca
     1081 aaacccgtct tggacgatgc ggatggcgat ggggtgacag accagttcga tcacgaaccc
     1141 aatacgcctg ccggctgccc ggtggatacg catggggtta gccgcgatac ggatggtgac
     1201 ggcgttccgg attgcaagga taaggaactg atcacaccaa cccagtgtca gccggttgac
     1261 gcggatggag taggcaagtg tcctgatccg gcctgttgca aggaaatcag cgcaagactg
     1321 gattcaggcg gagtgggttc catgggccgc agccggtgct ctatcggaga cctgcccagc
     1381 atcgtgttca agggaagatc tgttacccta agcaaggatg gaaaggcgtt ggctgccagc
     1441 attgcagata agatgaggga aaacccggcc tgtaaggttg ccgttatcgg ttatggcgaa
     1501 tccagcaaat ctgcccagca gctcagttgg gaccgggtga atgaagtgat caagtacctg
     1561 gttgaaaaag aaggcatcag ctctgaccgg tttatcttcc ggtatggtca gaccggtggc
     1621 gacgaaaaca ccattgatat taaagacggt acgggcgaag aaggacccaa tacggttccg
     1681 gctccgcatc cgaacctccg taagagataa atcagtcctc aggaataacc cgtggccgtt
     1741 ggatcaacca gcggccatcg gtttttcaga aagctgcatt ttaacataat ctgaagcgga
     1801 aacaccctga aaacgcccta ttttcgtttt tttacctaat ttttgtaatt ttgcaggctt
     1861 catgaaacca ctattcctgg cagtcgttat ttccgtaagc ctttcctcct gctttattct
     1921 tcccaaagcc tggcgtggca caggtcaggg cttttccaag ataatgaaga gcacggacta
     1981 tgactacaag ctgaaaatgg ctgaaaagta ttacctgaac aaggattaca atcatgccca
     2041 gcagctgtac gacgatcttt accgggtcat gaaaggttcg gagcattatg aggatatctg
     2101 gtataaatac gcttattcag cctattatct tcatgactac atcaatgcgg agaacctctt
     2161 taaaggattt gtggaagcat ttccaaacag tcccaaggcc gcggaaatgg actatatgcg
     2221 ggccttttgc ttttataagc aatccccgaa atatgaactg gatcagacca ataccacaaa
     2281 gaccattggt tttatgcagg cctttatcag ccagcacccg gaatccgaca aggtaaagga
     2341 tgccaccggg atcattgacc tttgccgggc aaaactggaa caaaaggatt ttgagaatgc
     2401 ccgcctctat tataaccttc gagctaccaa tacggcctat ttgaaggccg cctccctgac
     2461 gttcaacacc ctgatgaata cttatccgga ttcccagaaa agtgacgaat ataagttgta
     2521 tattatccgg gcggattacg aatatgccga gcggagtttt gaagataaaa agcaggcccg
     2581 gtttgagcag gtcgtaacgg attgtaacga tttcattgac cgttttccag atagtaaact
     2641 aaagaaacag gttcaggaat acctggacct aactaataac tcgctaaaag cactacaaaa
     2701 tgagcaggct cagaagacaa gttagtacac atatctccag taccgcagaa accaggaatc
     2761 tgaccgatct taaaaacaag accggcaacc tctatgaatc gattgccatt attgccaaaa
     2821 gagcaaacca gatcaatatt tccatgaagg aggagctgca cagcaaactg gaagaatttg
     2881 ccacgcatac ggacagcctg gaagagattc acgaaaacaa ggagcagatc gagatttcca
     2941 aagcgtacga gcggatgcca aacgctgccc tgctggctac ccaggaattt atggaagaca
     3001 agatctatta ccggaagaac gaggacgatc tcttcagcta agccctaatt tatattcatg
     3061 gtaaagggga aaaaaatact ccttggcgta tgtggcagca ttgccgccta caaatccatc
     3121 ctgctcaccc ggttgcttgt cagggccgga gcggaggtaa aagtgattct tactccttca
     3181 gccaaagatt ttgttacccc cctttctctt tccaccctat ccagaaatga agtgctctca
     3241 gatttgttcc gggaagataa ctggtccaat catgtggagc tgggccgctg ggccgatctc
     3301 ctgctgatcg ctccgctaag ctgcaacagc ctggctaaaa tggcgaacgg ccattgcgat
     3361 aatcttctgc tggcggtctg gctttctgcc aattgtcccg tagcctttgc gcctgccatg
     3421 gacgaagaca tgtggctgca tccggccacc cggtcgaata tagaaaagct ccagtccttt
     3481 ggaaattacc tggtcccggc ggaaaaaggc gaactggcga gcggtcttta cggggaaggc
     3541 cggatggccg aaccggaaaa catcctttct tttattgaaa agaatttttt ttttagcagg
     3601 gaattaaacg ggaagaaagc gctggtgacg gccggcccca cccaggaatc cctggatccc
     3661 gtgcgctata tttcaaatca ttcaacgggt aaaatgggca ttgcccttgc gcgcgagctg
     3721 gcgctccacg gggcggaagt ggaactggtg atcggtcctt ccgcactgcc cgtaaatttt
     3781 cctgcggtga aggtggtccc ggttcaatct gccgaagaga tgtaccaggc ctgcctggaa
     3841 cgattcccgg gaacggacat cgccgtgctg tccgctgccg tatcggacta tatgcccgtt
     3901 tccttctctc cggaaaaaat aaaaaagaat gcccagacct tcagcctgga actcacccgt
     3961 acaaaggata ttttaaagac cctgggagaa cgaaaaagaa cggggcagct cctcgtgggt
     4021 tttgccctgg agaaccggga tgaagaaacg aatgcccgga aaaaactgga atcaaaaaat
     4081 gcggacatga tcattttgaa ttccctgaat gaccccgggg ccggattcgg ttttgacacc
     4141 aaccgcgtga ccatatttga aaaaagcggc ccggaaatcc ggtatgatca aaaatcaaaa
     4201 caacaagttg ccaaagatat cgttgaccgg attataaaat tgtcgtgatg cgtacccgga
     4261 tccttttatg gttcgttttt tccagcctgg cttttacctc cgtccgggcc caggagctca
     4321 aggccaatat aacggtcctg tctaaccgca ttggatccac ggtgaataaa caggtcttcc
     4381 agaccctgca gaccgcgctc tatgagttcc tgaacaaccg caaatggacc aatgaacatt
     4441 ttaacgatga ggaaaagatc ccctgcaact ttttactgaa cctg
//
LOCUS       DASYCU010000472         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1986041, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000472 DASYCU010000000
VERSION     DASYCU010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986041"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..318
                     /locus_tag="VGM24_09545"
     CDS             181..318
                     /locus_tag="VGM24_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155663.1"
                     /translation="MKQEMDSSANAPAKVFVKLTVFGFVLIGFAYISLLLLGNVLKAI
                     H"
     gene            complement(372..1694)
                     /locus_tag="VGM24_09550"
     CDS             complement(372..1694)
                     /locus_tag="VGM24_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY4155664.1"
                     /translation="MKLIISCFSFFFLTLLLQAQTNYQPSAENLAARKWFDSARFGMF
                     IHWGAFSVPGEGEWVMNNRNIPVAEYKRLQTIFNPVRFDAEKWVSAAKRAGMQYITLI
                     TRHHDGFSNWDTKQSDWKITNTDWKKDAVKLIADECRRQGIKLFVYYSLLDWSRSDYQ
                     YETGRTGHGTGRTKKSDWDSYIRFMKAQLTELLTNYGPIAGVWFDGYWDQLSNDADKS
                     GRSNVDWHLPEIYALIHHLQPQCLIGNNHHLTPLPGEDFQMFEKDLPGKNTTGFGGQS
                     VSNLPLETCETMNNSWGFNITDTHYKSTREMIRLLVNDAGYGANLLLNIGPLPNGEIQ
                     TEFTDRLDSMGNWLKENGETIYGTHAGFIEPQPWGAVTEKGDQVYLHLFKTDGDKFFL
                     KIPYKVKSAKISGKTVPFQRLADNYLMFDLKGMSLDPVDAIIRLEVQK"
     gene            complement(1726..2712)
                     /locus_tag="VGM24_09555"
     CDS             complement(1726..2712)
                     /locus_tag="VGM24_09555"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEY4155665.1"
                     /translation="MRQIAFREALREAMAEEMRRDDRVFLMGEEVAEYNGAYKVSQGM
                     LDEFGPKRVIDTPISELGFTAIGVGAAQNGLRPVLEFMTWNFAVLALDQILNTASKML
                     AMSGGQIGCPIVFRGPNGSAGQLGAQHSTAFESYYANIPGIKLVSPSNGYDAKGLLKQ
                     AIRYEEDPVIFMESEVMYGDKSEIPEQEYYIELGKASVKKQGTDVTIVSYNKMVKVAL
                     GAAAELEKENISAEVIDLMTIRPLDWKTILESVKKTNRLVIVEEQWPYASISSEIAYT
                     IQKEGFDYLDAPVRRITAADAPLHYAANLVAAAMPSVEKTVKMVKEVMYLKK"
BASE COUNT          817 a          823 c          749 g          844 t
ORIGIN      
        1 tgagttccaa ttaccgcctg aagacaggaa aatctgaaaa tccgggacaa ggcagggcgg
       61 aatggcggga ttccgggaga atctaaccaa tatcgtaaaa ttacgttgca cattcagaat
      121 tcgtggctta ttttgtgacc ggcattattt tttgaaaaac caatattaaa attcgaccct
      181 atgaaacagg aaatggattc gtccgccaac gcacccgcga aggtttttgt gaaactgacc
      241 gtctttggtt ttgtattgat tggcttcgct tatatttccc tgctgttgct ggggaatgtt
      301 ttaaaagcca tccactaaat aaaataagtt cccggaccgg caaactgcct gtccgggatt
      361 tttttatttt attacttctg cacttcgagc cggataatgg catccacagg atccaggctc
      421 attcccttca gatcaaacat caggtaatta tcagccagcc gctgaaacgg aaccgttttt
      481 ccggatatct ttgccgactt cactttatag ggtattttca gaaaaaattt atccccgtct
      541 gttttgaaca ggtggagata gacctggtct cctttttccg taaccgctcc ccagggctgg
      601 ggttcgataa acccggcatg cgtgccataa atcgtttccc cgttttcctt aagccagttg
      661 cccatactgt ccagccggtc ggtaaactcg gtctgtatct ccccgttcgg gagaggaccg
      721 atattcaaca ggaggttggc gccgtaaccc gcatcgttta cgagcaggcg gatcatttcc
      781 ctggttgatt tataatgggt atccgtgata ttaaaacccc aggaattgtt catggtctca
      841 caggtttcca ggggcaggtt ggaaacagat tgtccgccaa aaccggttgt attttttccg
      901 ggaagatctt tttcaaacat ttgaaagtcc tctccgggca atggagtcag gtggtgattg
      961 ttaccgatca ggcactgggg ctgcaggtga tggatcagcg catagatctc cggcaggtgc
     1021 cagtcaacgt tcgaccggcc gctcttatcc gcatcattcg acagctggtc ccagtatccg
     1081 tcgaaccaga cccccgcaat ggggccataa ttcgtaagca gttcggtcag ttgggctttc
     1141 atgaaccgga tataactgtc ccagtcgctt tttttggtcc ggccggtccc atgaccggtt
     1201 cttccggttt catactgata atcgctccgg ctccagtcca gcagcgaata ataaacaaac
     1261 aatttgattc cctgccgccg gcattcgtcc gcaatcagtt ttacggcgtc cttcttccaa
     1321 tccgtgttgg tgatcttcca gtccgattgc tttgtatccc agttactgaa tccgtcgtgg
     1381 tggcgcgtga tcagggtgat gtactgcatg ccggcgcgtt tggctgccga aacccatttt
     1441 tcggcatcga agcgaaccgg attaaaaatc gtctgcaggc gcttgtattc ggctaccgga
     1501 atattgcggt tattcatcac ccactctcct tctccgggca cactgaaagc tccccagtgg
     1561 atgaacatgc cgaagcgggc gctgtcgaac cacttgcggg cagcgaggtt ttcggcagaa
     1621 ggctgataat tggtttgggc ctgcaggagc agcgtcagga aaaaaaatga aaagcaggag
     1681 attattaatt tcatcaattt cagatttgac gcattaaaga ccggatcatt tcttcaggta
     1741 catcacctct tttaccatct tcaccgtttt ttcaacagag ggcatggcgg cggcaacgag
     1801 gtttgcggcg tagtgcagcg gtgcatcggc agccgtgatc cggcggaccg gcgcatccag
     1861 gtaatcaaat ccctctttct ggatggtata ggcgatctcg gaggaaatgg aggcgtaggg
     1921 ccattgttct tccacgatca ccaggcgatt tgttttcttg acgctttcca ggatggtctt
     1981 ccagtccagc gggcggatgg tcatgaggtc aatcacttcg gcgctgatgt tctctttttc
     2041 gagctctgcc gctgcgccaa gcgcaacctt caccatttta ttataggaaa cgatggtcac
     2101 gtcagtaccc tgtttcttga ccgatgcttt tcccagttcg atataatatt cctgctccgg
     2161 aatttcgctt ttatcgccgt acatcacttc actttccatg aaaatgaccg ggtcttcttc
     2221 ataccggatc gcctgtttga gaaggccctt ggcatcatat ccattactgg gggataccag
     2281 ctttattccg ggaatattgg cataataact ttcaaatgcg gtagagtgct gggcgccgag
     2341 ctgtcccgcg gagccattcg ggccacggaa aacgatgggg cagccaatct ggccgccgct
     2401 catggccagc atcttggaag cggtattcag gatctggtcc agggcaagaa cagcaaagtt
     2461 ccaggtcatg aactccagca ccgggcgaag gccgttctgg gcggcaccga caccgatggc
     2521 cgtaaatcca agctccgaaa taggagtgtc tatcaccctt ttagggccaa attcatccag
     2581 catgccctgg ctcactttgt aagctccgtt gtattcagcc acttcttccc ccatcagaaa
     2641 aacccggtcg tcccggcgca tctcctcagc catggcctct cgtaaagctt cccggaatgc
     2701 gatctgtctc atttatatgt atttaagagg ggcaaaagta ttgaggaatg aggaatcggc
     2761 aatgggattt tcgttttatc gtcaatttat ccctgaaaat cgaaggtttg atccccttta
     2821 tcgaataagt tattaaaagg aaggaatgca cagcatcttt gtattgtcaa cgcgtcgaaa
     2881 aaatccaaac gaaataaacc tctaaacctt aaccctgaaa tccgtatcct gagaagaccg
     2941 aaacaaaatc cctaaatcct gaacccgtag aaaaatcctg aatccctgaa aaaaaccaat
     3001 ccgaatcctg agaagaacca aaccaagtcc caagagaaac caaaccaagt ccgaagagaa
     3061 accaaaccaa gtccaaagaa agcaaaccca agatcctgag aaaccaatcc gaccaatcct
     3121 ctgaaaaccg aatccattta gtatccatcc tgaggaaaac caaaaaaacc agtatcgtgt
     3181 aaagccggac ttttaatcat cctttaccat catgaaaatg tttacacttt gat
//
LOCUS       DASYCU010000473         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1258894, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000473 DASYCU010000000
VERSION     DASYCU010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258894"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VGM24_09560"
     CDS             <1..518
                     /locus_tag="VGM24_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155666.1"
                     /translation="ICFASMACENTMYDWSGIYIKQVLHGSNGMATIAFVIYMVAMTT
                     GRIFGDRMADKFGIKFVLRGSGILIFCGLLITVVSPYIPLTLIGYLFTGFGVSCVVPF
                     IFSLAGKIPMSNPGPALASISSIGYLGFLLVPPMIGYVAQSSSLRVSFGIIATMGLLI
                     IGLSRKINVPL"
     gene            complement(521..1270)
                     /locus_tag="VGM24_09565"
     CDS             complement(521..1270)
                     /locus_tag="VGM24_09565"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEY4155667.1"
                     /translation="MNRIASLKYFIAALIFLITQLPAGKLYSQKPSSAMEPLKVIKCN
                     DFPITGNGNNSEWEKTAWHPLTRLDSAGVNYATRFKILYSAKGIYVLFEGKDNRISTT
                     YDQDFQDLYNADVFVVFFHPDTTMPLYLEYEINAISKELVLLVPNLNGRASGWLPFHY
                     TGRRQVQKMVSVVGGKTEMGAAIKSWSAELFFPYAIFDPLLNVPPVSGTVWHANFYRL
                     DYDSGSMVKWSWAPIEHSFHEFRKFGSIRFE"
     gene            complement(1275..>1770)
                     /locus_tag="VGM24_09570"
     CDS             complement(1275..>1770)
                     /locus_tag="VGM24_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155668.1"
                     /translation="VVYNVAKMADLMDIKMEADIPWISFYDEGTGIGFAGIQLSYANA
                     GLESAPRLLNPYFYITAGPWIYWARALSLSFLASNMQQMIPAMKGNFFSEKWAYLVYE
                     TDKGDKPYEPVITWMKRLTHPLRVQLSEEVDDRVSRTVTELFVDKGKSGWENRETSTH
                     HTKK"
BASE COUNT          426 a          440 c          398 g          506 t
ORIGIN      
        1 tgatctgttt cgcttcgatg gcttgcgaga ataccatgta cgactggagc ggaatttata
       61 taaagcaggt attgcatggt tcaaacggca tggccacaat tgctttcgtc atttatatgg
      121 tggcgatgac gacgggcaga atattcggcg accgcatggc agataaattc ggcatcaagt
      181 tcgtgctgcg tggaagtgga atacttattt tctgcggcct gctcatcacg gtggtttctc
      241 cttatatacc cctcaccctg atcggctacc tgtttaccgg ttttggcgta tcctgcgtag
      301 tcccatttat ttttagtctg gccggaaaaa tacccatgtc caatcccggt ccggccctcg
      361 cttccatctc ttccatcggt tatctgggat ttttactcgt cccgcccatg attggctatg
      421 ttgcgcaatc ctccagcctg cgggtatcct ttggcattat tgccaccatg ggactattga
      481 tcatcggtct gagcaggaaa ataaatgtgc cgctgtaaga ttattcaaac cggattgacc
      541 cgaattttct gaattcgtga aaactgtgtt cgattggagc ccaggaccat ttaaccatgc
      601 tcccgctgtc gtaatcaagc cggtaaaaat tagcatgcca gaccgtgccg cttaccggcg
      661 gaacgttcag taacggatca aagattgcgt aggggaaaaa caattcggcg ctccaggatt
      721 tgatggcggc acccatttct gttttgcccc ccacaacaga taccattttc tgcacctgcc
      781 tcctgccggt ataatgaaaa ggaagccatc cggacgccct tccgttcaga ttcggaacaa
      841 gcaaaaccaa ttccttgctt attgcattga tttcgtattc caggtataag ggcatcgtcg
      901 tatcgggatg aaaaaaaact acgaacacat cggcattata tagatcctga aaatcctgat
      961 cgtaggttgt gctgatcctg ttgtctttcc cctcaaagag cacgtaaatt cctttggcag
     1021 aatacaatat tttaaaacgg gtggcatagt ttactcctgc ggaatcgagc cgggtcagcg
     1081 gatgccaggc tgttttttcc cattcggaat tatttccgtt tccggtaatc gggaagtcat
     1141 tgcattttat aacttttaat ggttccattg cagaggaagg cttttgcgaa tacagtttcc
     1201 cggcaggaag ctgcgtgatc aaaaagatca gggcagcaat aaaatatttc aggcttgcaa
     1261 ttctgttcac ggtcttattt ttttgtgtga tgtgtgcttg tttcccggtt ctcccagccg
     1321 ctcttgccct tgtccacaaa taattccgta actgttcttg aaacccggtc gtccacctct
     1381 tctgacaatt gcaccctcaa cggatgggtg agcctcttca tccaggtgat caccggttcg
     1441 tagggcttgt ctcctttatc cgtttcataa accagataag cccatttttc ggagaagaaa
     1501 tttcctttca tggccggaat catctgctgc atgttggagg cgagaaaact cagcgacaat
     1561 gcgcgtgccc agtaaatcca tggaccggcc gttatataga aataaggatt gagcagtcgc
     1621 ggtgcggatt ccagccccgc attggcataa ctgagttgta tacccgcaaa gccaatacct
     1681 gtgccttcat catagaaact gatccagggg atatccgcct ccattttaat atccataagg
     1741 tccgccatct tcgctacatt ataaaccaca
//
LOCUS       DASYCU010000474         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1986388, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000474 DASYCU010000000
VERSION     DASYCU010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986388"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGM24_09575"
     CDS             <1..685
                     /locus_tag="VGM24_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY4155669.1"
                     /translation="QLQHDLENKLQETLNIAEEIQAREKQVEVQVKQAGERASLLSEG
                     RKAQVSGLEEKVNGLLKKVGMPNARIRVKLSETGLNESGKDEVEFLFDANKSNRFEPL
                     KKVASGGELSRLMLCIKSLVAESMDMPTLLFDEIDSGISGEAARQVGIILRDLSKKRQ
                     VICITHQPQIAGKGDAHFYVFKEIREGKMHTRIRLLDNEARIVVIAKMLSGEKPTAAA
                     LENARELVN"
     gene            complement(722..1444)
                     /locus_tag="VGM24_09580"
     CDS             complement(722..1444)
                     /locus_tag="VGM24_09580"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY4155670.1"
                     /translation="MKKIILLGVAAVYLVSCGRFLGKRIRGNGVIKTEERTVSPFKNV
                     EASGAIKLMVSQGDLKPVKLEGDENILPYIEIRQEGDRIEVRTRPGYNISPTNDLIVY
                     VTAPVYNSIEVSGASDIKGQNKISNAENLQLEASGAGDIEMDVDAPEITAKISGSGSI
                     KLKGQTRTLDIDLSGAGHAYCYDLLSENTRVDISGAGSAEVFASVKLEAEVSGVGNVS
                     YKGNATNVNQHVSGAGSVSKAE"
     gene            1607..>2125
                     /gene="guaA"
                     /locus_tag="VGM24_09585"
     CDS             1607..>2125
                     /gene="guaA"
                     /locus_tag="VGM24_09585"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY4155671.1"
                     /translation="MTEKILILDFGSQYTQLIARSVREANVYCEIVPYHQKLDPDPSI
                     KGIILSGSPASVNDPDAPVVNVKALHSSLPILGVCYGAQLTAREFGGKVAGSSKREYG
                     RALLNKLKEDVLLKDVSPLSQVWMSHADTILELPAGFDVLATTESIPYAAFKNNGEGY
                     PLYCLQFHPEVYH"
BASE COUNT          512 a          554 c          545 g          514 t
ORIGIN      
        1 ccaattacag catgacctgg aaaataagct tcaggaaacg ctgaatattg ctgaggagat
       61 ccaggccagg gaaaaacagg tggaagtgca ggtgaagcag gccggggaac gcgcttccct
      121 tttatcggaa ggacgaaaag cacaggtgag cggcctggag gaaaaagtaa acggattgct
      181 gaagaaagtg ggcatgccga atgcccgtat ccgggttaaa ttatccgaaa ccggtttgaa
      241 cgaatccgga aaggatgagg ttgaattttt gtttgatgcc aataaaagca accggtttga
      301 acccctgaaa aaagttgcct ccggcggaga gctcagccgg cttatgctat gcataaaatc
      361 gctggtggct gaatccatgg atatgccgac gctgttattc gatgaaatcg attccggaat
      421 ttccggtgaa gccgcccggc aggtgggcat catcctgcgg gatctttcca aaaagcggca
      481 ggtcatctgc ataacgcatc aaccccagat tgcgggaaaa ggagatgcgc atttttatgt
      541 gttcaaggaa atccgggaag ggaaaatgca cacccggatc cggctgctgg ataatgaagc
      601 gcggatcgtg gtcattgcca aaatgctttc gggagaaaaa cccaccgcgg cggcattgga
      661 aaatgcacgg gaactggtga attgaaaagg cgggatccgt tttgcatttc ggatcaggcg
      721 gtcattctgc cttgctcaca ctccccgcac cactaacatg ctggttcaca ttggtagcat
      781 ttcctttata gctcacattc cccactccgc tcacctccgc ttctaatttt acgctggcaa
      841 atacctctgc acttccggcg cccgagatat ccaccctggt attctccgaa agcaggtcgt
      901 aacaataggc atggcccgcg ccgctcagat cgatatcgag ggtcctggtt tgtcctttca
      961 gcttgatgga accgcttccg gaaattttag ccgtgatttc aggagcatcc acgtccattt
     1021 caatatcccc ggcgccgctg gcttccagtt gaagattctc tgcattggaa atcttgttct
     1081 gccctttgat atcgctcgct ccggacacct cgatgctgtt gtacaccggg gcggttacgt
     1141 atacgataag atcgttggtc ggactgatat tatatcccgg ccgggtcctc acttcgatcc
     1201 ggtctccctc ctgcctgatc tcgatatagg gcagtatatt ttcatcgccc tccagtttta
     1261 cgggtttaag gtccccttgt gaaaccatca gtttgatggc cccgctggcc tccacgttct
     1321 tgaaaggaga aacggttcgc tcctcggttt taatgacccc gtttccccgg atgcgtttac
     1381 ctaaaaaacg gccgcaggaa acgaggtata cggcggctac ccccagcagg attatttttt
     1441 tcatggttgt tttttgatat acaaaagttc ggatttattc ccgattagaa aaatcatttc
     1501 gggtattgga cggcgccggc cggggaaagg ttacggaccc cccgggaccg ggaaatccac
     1561 aggggaaagg gcaaacgcca aaacctttct ttatcttcgc aactccatga ctgaaaagat
     1621 tctaatcctc gatttcggtt ctcagtatac ccagttgatt gcccgctccg ttcgtgaagc
     1681 gaatgtgtat tgcgagatcg ttccttacca tcagaagctg gatcccgatc cctccattaa
     1741 aggcatcatt ctttccggat ctcctgcttc cgtgaacgac ccggatgcgc cggtggtgaa
     1801 cgtaaaggcc ctccattcct ccctccccat tttgggggtt tgctatggag cgcagctgac
     1861 cgcccgcgaa ttcggcggaa aagtagccgg cagcagcaaa agagagtacg gcagggccct
     1921 gctgaacaag ctaaaggagg atgtgctgct gaaagatgtc agtccgctca gccaggtctg
     1981 gatgagccat gcggacacca ttcttgaact gcccgcaggc tttgatgtgc tggccaccac
     2041 ggagtccata ccctacgccg catttaaaaa taacggggaa ggatatcccc tatactgcct
     2101 ccagtttcat ccggaagtat atcat
//
LOCUS       DASYCU010000475         6585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2566069, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000475 DASYCU010000000
VERSION     DASYCU010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2566069"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VGM24_09590"
     CDS             complement(<1..501)
                     /locus_tag="VGM24_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4155672.1"
                     /translation="MLDLNGRSIFITGGTGSFGKEFTKIILQRYPSIKRLVIFSRDEQ
                     KQFQMAQEFPPDAYPMIRFFIGDVRDHERLKSAIKGIDTVIHAAAMKHVPIAEYNPME
                     CIKTNIMGAENLIRACLESDVENVVALSTDKAAAPINLYGATKLASDKLFIAANNIKG
                     TKNIRFS"
     gene            complement(501..1391)
                     /locus_tag="VGM24_09595"
     CDS             complement(501..1391)
                     /locus_tag="VGM24_09595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4155673.1"
                     /translation="MKGSVLLRRAYNKFRYLFGDKSNAEIINFKKEIWQTDELSKSFI
                     RGSDANVLPVAEIMDREVNEFFLSECKPQDKVLDIGCGHGIVSEFLATRGIQMFSVDI
                     SEKLLSEFRSRIKDKHLSIDIKQGDAYHIPYPNEAFDVVVARMFLPHFPDWPVVLKEM
                     ARVTKPGGKLLVHFSSAENSELGKRLGKNDCVFVTSPVSKKGWDFYAETENKELSKVS
                     NSLGLKVTGVTPVSFFLHNRILGYALGTGGYNSYMEKVQEFFKDEKVREFVIWFDKEI
                     ISKCSPALTHFNIINFKKLK"
     gene            complement(1393..2688)
                     /locus_tag="VGM24_09600"
     CDS             complement(1393..2688)
                     /locus_tag="VGM24_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4155674.1"
                     /translation="MSSSVIQVENLSKQYRLGEVSTGTIAHDLNRWWHVVRGKDDPYL
                     KIGDENDRSQKGSSDYVWALQGINFKVQQGEVLGIIGRNGAGKSTLLKILSRTTAPTA
                     GSVKIKGRVASLLEVGTGFHPELSGRDNIFLNGTILGMTKSEIRKKFDAIVDFAGVER
                     YIDTPVKRYSSGMYVRLAFAVAAHLEPEILIVDEVLAVGDAEFQKKCIGKMKEVSDRD
                     GRTVLFVSHNMAAISALCSRAILLNNGIVSFNGPTPEALREYIFSNKLEAHGQVIFNG
                     AERERFGEKRYATWKTIQLLNQDGLVSDTFLMGEKMKLSLELEVHKPSEDFEIGIGIG
                     NLQEVFTHYLVSTWEGVSAIRNTGKTTVEVEIPRIFLFPGDYLINIWICLKGAYYDDA
                     IHNAINFRVEEGKLNEHDTYFERFSKNTQVYTPSYWKIN"
     gene            complement(2702..3556)
                     /locus_tag="VGM24_09605"
     CDS             complement(2702..3556)
                     /locus_tag="VGM24_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4155675.1"
                     /translation="MDKLSEDWDLVIKPKTAFFDLHLGDIWRYRDLLGLFVKRDFAAQ
                     YKQTILGPIWHLIQPILTTAIFLLLFGKIANIPTDGIKPVLFYMSGITMWNYFAACLT
                     NTSNTFVANAAIFGKVYFPRLVIPLSIVLSNIVRFGIQFGLLLAAMIWYQFNGFPMHI
                     SILWLWIPVLMILMAGIGLGLGIIISSLTTKYRDFVILMAFVVQLLMYVTPIGYPLSF
                     LANSKYKTIISLNPLTGVVETFRYALFNKGSLHLYPLLYSFIFMIISVFLGLLIFSKV
                     EKTFMDTV"
     gene            complement(3604..6015)
                     /locus_tag="VGM24_09610"
     CDS             complement(3604..6015)
                     /locus_tag="VGM24_09610"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEY4155676.1"
                     /translation="MLESPEGLDHLKTTNNQMSWKLGPRDFVLKNLKYIPWIIICGVI
                     AWVLAFLNIRYSTPIYSAQSSMLISNESIGRPDRFDAMLMTSSGSNLSNEIEILSSRP
                     VLQRVVRNMNLLTRYYNIGKVRTSMVYPEAAVTLEIVSLTSPEATFTIQITILNDRQY
                     VIGKNPKPIAFGQIININGNMVRLTRNLDFDLKSLGSLSYFIMHQTPSEVANDYLGAL
                     KIVQANDQATILTLSITTENTALGTDFLNALMTVYDSLNIEDKNRIATNSLNFINKNL
                     DTLSQQISGLEGNVRTFRVNNDVFDEGDQSRMYLSNVEGTQAGIDGQDVKISLANVLQ
                     KYINDRRYSHELVPINMGIEEPALTQRIQEYNRLQLERDNNLKTTTANNPLIVAYDSS
                     LETIRRSMSEALNNIKNGYEIAKRKMMEQRDQVTGKLRALPGKTIQLTNVARRQKILE
                     ELYTLLLQKKLETSISSASTISNSKVVEPALASGNPISPNKNKIYSVYLLFGLLIPVG
                     VIAIKELLRDKVNNRMDVEKLTQTPILGEIGHSDDEEPLVVLRNSRRFVAEQFRILRS
                     NLKYMTVKKENPSILVTSSFSGEGKSFISINMASVLALSGKKTVIMEFDIRKPKVMEG
                     LGIKRKMGITNYIIGRAQLKDIIVQVPGFDSLFVIACGPIPPNPAELLLDNKLDELMT
                     EVRQNFEVIIMDTAPVGLVSDSVSLGKYADCTLFVVRMGHTYRKIMRLVEDLYKEKKL
                     PGMSLILNDVKLEGGYYSGYYGGYGYYGGYGYGIESGYFEKPAKKKMVPIHKQIARWW
                     GKWFG"
     gene            complement(6031..>6585)
                     /locus_tag="VGM24_09615"
     CDS             complement(6031..>6585)
                     /locus_tag="VGM24_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEY4155677.1"
                     /translation="TGMAQANGGSAPSAPGYQVDENGDIVFQGLGHLHVEGLTKALLK
                     DTLDARLSTFLQHPYYNIRFLNYKFTVLGEVGRPGIITIPGERINLLEAIALAGDLTF
                     FARRDNVEVIRETNNKREFARLDLTKPEIMKSPYFYLQQNDLVIVEPTRKKVSANDQT
                     VVRNVSLAATVVSLFAVIYSIFRR"
BASE COUNT         1754 a         1634 c         1366 g         1831 t
ORIGIN      
        1 cgaaaacctt atattcttgg ttcctttaat attgtttgcc gcaataaaca acttgtcgga
       61 tgccagtttg gttgctccat acaaattgat cggagccgct gccttgtccg tggaaagcgc
      121 tacaacattt tcgacatccg attccaggca ggcccggatc aggttctcgg cacccataat
      181 attggttttg atacactcca tcggattgta ttctgcgatc ggaacgtgtt tcatggctgc
      241 agcatgaata acggtatcaa tcccctttat cgccgatttc aacctttcgt gatcccttac
      301 atcgccaata aaaaaccgga tcatgggata ggcatctggc gggaactcct gcgccatctg
      361 aaattgcttc tgttcatccc gggagaaaat aaccagtcgt ttaattgatg gatagcgctg
      421 aaggatgatt ttggtaaatt cctttccaaa ggaacctgtt cctccggtaa tgaaaatcga
      481 tctgccattt aaatcaagca ttactttaat ttcttaaaat tgattatatt aaaatgcgtt
      541 aaagccggtg aacattttga aataatctct ttgtcaaacc aaatgacaaa ctcacgcacc
      601 ttttcatcct taaaaaattc ctgaaccttt tccatatagc tattatatcc acccgtaccc
      661 agggcatagc ccaaaatgcg attgtgaagg aaaaaactca caggggtcac tccggttacc
      721 ttcaaaccca gggaattgga gaccttgctc aattctttat tctccgtttc ggcataaaaa
      781 tcccagccct tttttgaaac cggactggtc acaaagacgc aatcattttt accgagtctt
      841 tttcccagtt cggaattttc ggccgaagaa aagtgcacca gcagtttacc acccggtttc
      901 gttacccggg ccatttcctt tagtacgacc ggccaatccg gaaaatgggg caggaacatc
      961 ctggcaacaa ccacatcaaa tgcctcgtta gggtaaggaa tatggtatgc gtccccttgc
     1021 ttaatatcaa tggaaagatg cttatccttt atcctggatc tgaattccga caataatttt
     1081 tcactgatat caacggaaaa catttgtatt ccgcgggtcg ccagaaattc gcttacgatc
     1141 ccatgcccgc acccaatatc aaggacctta tcctgaggct tacattcgga aagaaaaaat
     1201 tcgttcactt ccctgtccat gatctcagca accggtaaaa cattcgcatc gcttcccctt
     1261 ataaaagact tcgaaagctc atctgtctgc catatttcct ttttgaaatt aattatctca
     1321 gcattactct tgtcaccgaa taaatacctg aacttattat aagccctcct gagcaatacg
     1381 ctgcctttca tttcaattaa ttttccagta agaaggtgtg taaacctgtg tattttttga
     1441 gaaccgttca aaataggtat cgtgttcatt cagcttgccc tcttccactc tgaagttaat
     1501 ggcattgtga atggcatcgt cataataagc tcctttcagg caaatccata tatttattaa
     1561 gtaatccccc ggaaagagaa aaattctcgg gatttccact tctactgtcg tctttcctgt
     1621 attccggata gcggaaacgc cttcccaggt ggaaaccagg taatgcgtaa acacctcctg
     1681 gaggtttcca ataccaatac caatttcaaa gtcctcactg ggtttatgga cttcaagttc
     1741 cagggacaat ttcatttttt ctcccattaa aaaagtatcc gaaaccaggc catcctggtt
     1801 aagcaactgg atggtcttcc aggtggcgta ccttttctcc ccaaaccgtt cgcgctctgc
     1861 cccgttaaaa ataacctgac cgtgcgcttc cagcttattt gaaaaaatat attctctcag
     1921 cgcttcaggg gtaggaccgt tgaaggagac aattccgtta ttgagcaaaa tcgcccgact
     1981 gcacagggcc gaaatcgcgg ccatattatg gctgacaaat aacacggtcc gtccgtcacg
     2041 gtcgctcact tctttcattt tcccaataca ttttttctgg aattcagcat cccctaccgc
     2101 cagcacttca tcaacgatca ggatctccgg ttccaggtgg gccgccacgg caaaagccaa
     2161 tcttacatac atgcccgaag aatatctttt aaccggggta tcgatatatc gctcaacgcc
     2221 ggcaaagtct acaatagcgt caaatttttt tctgatttct gatttggtca tacctaaaat
     2281 ggttccattc agaaaaatat tgtccctgcc gcttaattcc ggatggaaac cggttcccac
     2341 ttccaaaaga ctggccaccc tgcccttgat tttgacggat ccggctgtcg gcgctgtggt
     2401 acggctcaat atttttaata aagtactctt ccctgcgccg tttcttccga tgattcccag
     2461 tacctcaccc tgctggacct tgaaatttat accctgcagc gcccacacgt aatcgctgct
     2521 gcctttttga cttcgatcat tttcatctcc tatcttaagg tagggatcat ccttaccgcg
     2581 caccacatgc caccatcggt ttaaatcgtg agcaatcgtt ccggtactta cttctcccag
     2641 acgatattgt ttggagagat tctcaacctg aataacagaa gaagacattt attcgaaata
     2701 tttaaaccgt atccataaag gttttttcca ctttactgaa gatcagcaat ccaagaaaaa
     2761 cagaaatgat cataaaaatg aagctgtaca gaagcggata taaatggagg cttcccttat
     2821 taaatagagc ataacggaat gtttctacca cgcccgtcag cggattgagg ctgataatcg
     2881 ttttatactt gctgttggcc agaaaagata gcggatatcc gatcggggta acatacatca
     2941 gcaactgcac gacaaacgcc ataagaataa cgaagtcgcg gtatttggtg gtcagggagg
     3001 aaataatgat tcccagcccc agaccaatgc cggccatcag gatcatcagg accggtatcc
     3061 ataaccataa aatgctgatg tgcatcggaa agccattaaa ctgataccag atcatagccg
     3121 ccagcagcag gccaaactgt attccaaaac ggacgatatt ggaaagcaca atcgataagg
     3181 ggatcaccag gcggggaaaa taaaccttgc caaatatggc ggcgttggct acgaacgtat
     3241 tggacgtgtt ggtcaggcag gcagcaaaat aattccacat cgtgatcccg ctcatataaa
     3301 acaaaacggg ctttattccg tcggtcggga tattggcaat cttcccgaaa agcaataaaa
     3361 agatcgctgt ggtgagtatc ggctgaatga ggtgccagat ggggcccagt atggtttgct
     3421 tgtactgtgc ggcaaaatcg cgcttgacaa aaagacccaa taagtcccgg tatctccaga
     3481 tatcaccgag atgcagatca aaaaaggccg ttttcggttt tataaccagg tcccagtctt
     3541 ctgacaactt gtccattttt cagctaccca ttgttttaaa aaaatcaacc tgctccttga
     3601 aaattaacca aaccattttc cccaccaacg ggcgatctgt ttatgaatgg gcaccatttt
     3661 ctttttggcg ggtttttcaa aataaccgga ttcgattcca taaccataac cgccataata
     3721 accatatccg ccataatacc cgctgtaata acctccctcc agtttgacat cattcagaat
     3781 gagagacatc cccggaagtt ttttctcttt gtacagatct tctaccagtc gcataatttt
     3841 ccggtaggta tgacccatcc tgaccacgaa cagggtacaa tcggcatatt tcccgagcga
     3901 tacggagtca ctgaccaggc caaccggcgc cgtatccata atgatcactt caaaattttg
     3961 cctcacctcc gtcatcagct catccagctt gttatccagt aagagctcgg ccggattggg
     4021 tggaatggga ccgcaggcaa tcacaaacaa actatcaaag cccggcacct gcacaataat
     4081 gtcttttaac tgggcccgcc cgataatata attcgttatg cccatcttcc tttttattcc
     4141 aaggccttcc atcactttag gtttgcggat atcaaattcc atgatcaccg ttttcttgcc
     4201 gctgagggca agaaccgatg ccatattgat actgataaag gatttcccct ctccgctgaa
     4261 ggaagaggtt accagaatgg aaggattttc cttttttacc gtcatgtact tcaggttgct
     4321 tcgaaggata cggaattgtt cggccacaaa ccgccggctg tttcgaagca caaccaacgg
     4381 ttcttcgtcg tccgaatgac cgatttctcc cagtatcggg gtttgggtga gtttttccac
     4441 atccatccgg ttgttcacct tatcccttag caattccttg atggcaataa cccccaccgg
     4501 gataagcagg ccaaaaagca gatacacgct atagatctta ttcttattcg ggctgatcgg
     4561 atttccggag gccagtgcgg gttcaaccac cttggaattg gaaatagtgg aagccgatga
     4621 aatagatgtt tccagcttct tctgtaagag caaggtgtac aattcctcca atatcttttg
     4681 ccggcgggcc acattggtca attggatggt tttccccgga agtgccctta attttccagt
     4741 cacctgatcc ctctgttcca tcattttcct cttggctatt tcatagccgt tctttatatt
     4801 attcagcgcc tccgacatac tccgccggat ggtttccagg gaggagtcat aggcgacgat
     4861 cagcggatta ttggccgttg tcgttttcag attgttgtcg cgctccagct gaagccggtt
     4921 gtattcctgg atgcgctggg ttaaagcggg ttcttcaatg cccatgttga tcggcacgag
     4981 ttcatgagaa taccttctgt cattgatata tttctgcaat acattggcaa ggctgatttt
     5041 tacgtcctgc ccgtcaatgc cggcctgggt tccttccaca ttggataaat acatcctgga
     5101 ctgatccccc tcgtcaaaaa catcgttgtt cacccggaaa gtgcgcacat tcccttccag
     5161 gccgctgatt tgctggctca gggtatccag gtttttatta ataaaattga ggctgttggt
     5221 ggcaatgcgg tttttatcct caatgttcag ggaatcgtaa accgtcatga gcgcattcaa
     5281 gaaatccgtg cccagcgccg tattttcagt tgtaatcgac agcgtgagga tggtggcctg
     5341 gtcattggcc tgcacgatct tcagcgctcc cagatagtca ttggcgacct ctgaaggagt
     5401 ctgatgcata ataaaataag aaagacttcc caggcttttc aggtcaaagt ccaggtttct
     5461 ggtcagccgg accatattgc cattgatatt gattatctgg ccaaacgcaa tcggcttcgg
     5521 gttttttccg attacatact gccggtcatt taaaatggtg atctgaatag tgaatgtagc
     5581 ctccggactg gtcaatgaaa cgatttcaag ggtaaccgcc gcttcaggat aaaccatgga
     5641 ggtccttact ttgccgatgt tataataacg cgtgagcagg ttcatgttcc ggaccacccg
     5701 ctgcagaacc ggccgggaag aaaggatctc tatctcattg ctcagattcg aaccggaaga
     5761 ggtcattaac atggcatcaa agcgatcggg ccttccgatt gactcattgc tgatcagcat
     5821 ggaagactgc gcactgtaaa tgggtgtgct gtaccggata ttcaaaaagg caagaaccca
     5881 ggcgattacc ccgcagataa tgatccaggg aatatatttc aggtttttta aaacgaaatc
     5941 tctcggtccc agtttccagg acatctgatt attggtcgtc ttcaaatggt ctaatccttc
     6001 cggggattca agcataattt tttattctga ttatcttctg aatatactgt aaataacggc
     6061 aaagagcgag accaccgtgg ccgccagact gacgttgcgg acaacggtct ggtcgtttgc
     6121 agacactttc ttcctggtcg gctctacaat aacaagatca ttttgctgaa gataaaaata
     6181 gggggatttc ataatctccg gcttggtcag gtccagcctt gcaaactccc gtttattgtt
     6241 cgtttcccgg atcacctcca cattatctct tctggcaaaa aaagttaggt ctcccgcaag
     6301 ggcaatggct tccagtaaat taatccgctc gcccggaata gtaatgattc ccggacggcc
     6361 aacttctccc aatacggtaa acttgtaatt taagaaacgg atattatagt aggggtgctg
     6421 gagaaaggtt gataagcgtg cgtcaagggt atcctttaat aaggcttttg tcaatccttc
     6481 aacatgtaaa tgacccagcc cctgaaaaac aatatcaccg ttctcatcaa cctggtaccc
     6541 gggcgcagag ggagccgatc caccgtttgc ctgagccatg ccggt
//
LOCUS       DASYCU010000476         6615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5176416, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000476 DASYCU010000000
VERSION     DASYCU010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5176416"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /gene="glmM"
                     /locus_tag="VGM24_09620"
     CDS             <1..1381
                     /gene="glmM"
                     /locus_tag="VGM24_09620"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY4155678.1"
                     /translation="ALIKSISGIRGTIGGKPNENLSPLDIVRFTSAYGTWIRENQSAS
                     RKIVVGRDGRISGEMVRNLVVSTLNGLGLDVVDLGLSTTPTVEIAVISEKAAGGIIIT
                     ASHNPKEWNALKLLNEKGEFISEQDGKWILDRALNETFSFASIDKLGSCRTDSSYLEK
                     HIQRILQYPLVDRQAIQNKNFKVVVDVINSTGALFIPPLLKALGVEQVTVLNEEVNGK
                     FSHNPEPLPENLSALSAEVKKTGAHLGIAVDPDVDRLCFVCEDGSMFGEEFTLVAVAD
                     YVLARKPGNSVSNMSSTKALKEITIRHGGEYYSSPVGEVNVVEKMKLVHAVIGGEGNG
                     GIIVPDLHYGRDALIGIGLFLSALAHHKNGMRSFRARYPDYFISKNKIELGKSIDVKE
                     LFSKIKSKYKNHPANTEDGLKIEFDEDWVHLRTSNTEPIIRIYAESNFETTANNIARR
                     LMKDIQEFI"
     gene            1494..2621
                     /locus_tag="VGM24_09625"
     CDS             1494..2621
                     /locus_tag="VGM24_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY4155679.1"
                     /translation="MERIYFDNAATTSLDPEVLEAMMPYLTEKFGNPSSIYSYGREGR
                     MAVEQARKTVSRLLNAHPAEIFFTSGGTESTNTAIAAAIYDLGCRNIITSPIEHHATL
                     HTVEYYRERGEASLHFVKLLSNGHVDLGDLEKRLSGMEPGCLVSLMHANNEIGNLLDL
                     KTAGEICKKYGAVFHSDTVQTVGHYPFDLKNTAVHFITGAGHKFHGPKGIGILYIREE
                     VKIHPYIHGGSQERNMRAGTENVYGIVGFAKALELASARMESEKKEIEKLKEYMASQL
                     KQVIPDSQFNGDPFGNCLYTILSVSFPKSEKSEMLLLNLDINHICASGGSACTSGAEQ
                     GSHVIRAIYPDTDRVTVRFSFNRHNSRQEVDQVVARLKEMI"
     gene            complement(2833..4347)
                     /locus_tag="VGM24_09630"
     CDS             complement(2833..4347)
                     /locus_tag="VGM24_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155680.1"
                     /translation="MKKSLILPVLILISTLVNAGCPVGSAPAVYGTNNVWIGYVFDGI
                     NFDTYKGYVNEGSSSSPNFDESFGGTNSAYNTLDGCSTTTETFSMRYKLAQSFVDGDY
                     TIVVGGDDGYRLSLDGGATWAINSWVLQSYTSTTLTVHLNGSTNLVLEYYENSGDNRV
                     SFNITKDCTTTGTQTVYGTGTKWLGYVYSGMNFDSYKGYITEGSSVSPNFDEGFGGDV
                     ASFATTGCSIVTTQFSVRFRLRTVLTSGVYTITVGADDGYRLSLNGGSTYVINKWSDQ
                     GYNTTTYTATLSGSYDVVLEYYENAVSNRVSFSMSGGSILPVTLTGFEGNLKSNHEVD
                     LGWKTMMESGIDHYEMERSGNGQDFTQIGRIGSQTTISTHDYQLSYHFTDSDPLQGIS
                     YYRIKVIGKDNYSNISPVVQVNNNEIRGIKIYPTLIQNNTVFVETDKTLRDARLEFFD
                     LSGKKISETSWESLNGRQNCQVARSNRLPSGTYVARLTANGQPVKNQLMIVQNN"
     gene            4512..5705
                     /locus_tag="VGM24_09635"
     CDS             4512..5705
                     /locus_tag="VGM24_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEY4155681.1"
                     /translation="MPSQTTASARIYSIDILRGAVMIIMALDHVRDFFHFTAATDSPT
                     NLASTTPALFFTRWITHFCAPVFVLLAGISASLMSRKRTRPQLSLFLIKRGLWLIGVE
                     VLLVTFAWTFNPFYNVFILQVIWAIGISMIILGIFIRYSSRQVILLAGLIIICCHNLL
                     DFPEAAGKGNIGFFWNLAHHAAFTVYPVFPNHEVIIVYAFLPWTGIMMTGFGIGKIFS
                     NEVRSGLRRRILFSSGFALLLLFFILRFINAYGDPVAWSVQRTPVFTLLSFLNVTKYP
                     PSLDYIGITIGTALIVLGLLEKVSGKSLKFLITFGRVPFFYYVVHLYLIHLTVFVFFI
                     LEKFPSKDIAAQSLPFYFRPDRFGFNLWIVYGIWAAIIPMIYPLCRWYNRYKSTHYHW
                     WLSYL"
     gene            complement(5707..>6615)
                     /locus_tag="VGM24_09640"
     CDS             complement(5707..>6615)
                     /locus_tag="VGM24_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY4155682.1"
                     /translation="YKKPELHTGMELELRDSRHPVIEKNLPPGEEYIPNDLFLDPETQ
                     QIIILTGPNMSGKSAVLRQTALITLMAHMGSFVPASRARIPLTDKIFTRVGASDNLSA
                     GESTFLVEMNETASILNNITPGSLIILDEIGRGTSTYDGISIAWSIAEYLHRSPFKPK
                     TLFATHYHELNELEDRFPGIKNFHITSREHGNKIIFLRKLAPGGSTHSFGIHVARMAG
                     MPADLIGRANEILKQLEESRSGDGSGLQEGLKKIAAPRLQLSIFDAHTQLFEEIRKSL
                     SELDINRLTPVEALLKLNEIKNMLRG"
BASE COUNT         1637 a         1587 c         1610 g         1781 t
ORIGIN      
        1 ggcattgatc aagagtattt caggaatcag aggaacaatc ggcggcaaac ccaatgaaaa
       61 cctcagtccc ctggatatcg tccggtttac ctctgcctac ggaacctgga tcagggaaaa
      121 tcaatcagcc tccagaaaaa tagttgtcgg gcgggacggc cgcatcagtg gcgaaatggt
      181 tcgcaacctg gtggtaagca ccctgaacgg actcggcctg gatgtggttg acctcggcct
      241 gtccactacg ccgaccgtgg aaattgctgt aatttctgaa aaagccgccg gaggaatcat
      301 catcacggcc agtcataatc ctaaggaatg gaatgccctc aaactgctaa acgaaaaggg
      361 tgagtttatc agcgaacagg acggcaaatg gatcctggac cgggccctga atgaaacctt
      421 cagctttgcc tccattgaca aactgggcag ctgcagaacg gatagcagtt acctcgaaaa
      481 acatatacag cggatattgc aatatccgct ggtggaccgc caggcgattc aaaataaaaa
      541 ctttaaggtg gtggtggatg tgatcaattc cacgggcgcc ctctttatcc cccctttgct
      601 aaaagccctc ggtgttgaac aggtaactgt tctcaatgaa gaagtgaatg gtaaattctc
      661 tcataatccg gagccgcttc cggaaaacct ctccgcattg agcgcagaag taaaaaaaac
      721 gggagcccac ctggggatag ccgtagatcc ggacgtagac cgcctgtgtt ttgtgtgtga
      781 ggacggcagc atgttcgggg aggaatttac cctggtcgcc gttgcggatt atgtgctggc
      841 ccgcaagccc ggaaacagcg tgagcaatat gagcagtacc aaggcgctga aggaaatcac
      901 gatccggcat gggggtgaat attattcttc tccggtggga gaggtgaacg tggtggaaaa
      961 aatgaaattg gttcatgcgg tgatcggcgg ggaagggaat ggcggaatca ttgttccgga
     1021 ccttcattac gggagggatg cgctgatcgg aatcgggttg ttcctgagtg ctttggcaca
     1081 tcataaaaac ggaatgcggt cgttccgggc cagatacccg gactatttta tttctaaaaa
     1141 taaaattgaa ctgggaaagt ccattgacgt gaaggagctt ttcagcaaaa tcaaatcaaa
     1201 atacaagaat catccggcga atacagaaga cggtttgaaa atcgaatttg atgaagattg
     1261 ggtccatctc cgcacgtcga acaccgagcc catcattcgc atctacgcgg agagtaattt
     1321 tgaaacgacg gccaacaata tcgcccggcg actgatgaaa gatattcagg aattcattta
     1381 gctttaaggg ccggcccagg aaatttttcc ggacccgttc atcatttccg gaacgccctg
     1441 gtttgcaaca tgaacccttt tcccattttc aaattatcta actggccccc ggtatggaaa
     1501 gaatttattt cgacaatgcg gcgaccacct cactcgatcc ggaagtgctg gaggccatga
     1561 tgccctatct gactgaaaaa ttcggcaacc cttcctcgat ttattcttac gggagggaag
     1621 gacggatggc agtggagcag gcccgcaaaa ccgtatcccg cctgctgaat gcccatccgg
     1681 cagaaatttt ttttacgagt ggcggaaccg aaagcaccaa cacggcgatc gctgccgcta
     1741 tttatgattt gggatgccgg aacatcataa cttctccgat tgagcatcat gccaccctgc
     1801 acaccgtgga atattaccgg gaaagaggag aagcttcgct gcattttgta aagctgcttt
     1861 ccaacggtca tgtcgacctc ggggatctgg aaaaacggct ttccggaatg gagcccggat
     1921 gcctggtcag ccttatgcat gccaataatg aaattggtaa cctgctggat ctgaaaacgg
     1981 caggagaaat ctgcaaaaaa tatggagcgg tttttcattc ggatacggta caaacggtcg
     2041 ggcattatcc ttttgatctt aaaaatacgg ccgtccattt tattacggga gccggccata
     2101 aatttcacgg gccaaaggga attggcatcc tgtatatccg ggaagaggtg aagattcatc
     2161 cctacataca cggggggtcc caggaaagga atatgcgcgc cggaacggaa aacgtttatg
     2221 gcattgttgg atttgcaaaa gcgcttgaac tggcttctgc ccggatggaa tcagagaaaa
     2281 aggagattga aaaactgaag gaatatatgg cttcccaact aaaacaggtc attccggatt
     2341 cgcaatttaa cggggaccct tttggaaact gcctgtacac catactcagt gtaagttttc
     2401 cgaaatcaga gaagagcgaa atgctcctgc ttaacctcga tatcaatcat atctgtgctt
     2461 ccggaggaag cgcctgtacc agcggggcgg agcagggatc ccatgttatc cgcgccattt
     2521 atcccgatac ggaccgggtt accgtgaggt tctccttcaa ccggcataat tccaggcagg
     2581 aagtcgatca ggtggtagcc agactcaaag aaatgatatg aacgccggga aataaaaaaa
     2641 cccagtcatt ttatgctgga tctttttatg tgttttgagt aagaaatttt ctaaagggaa
     2701 ccagatggat cttggaccaa aaacgggctc ttcagttgca aatttaaggc tattctgatt
     2761 tggagattca attcgttata aaagtttctc acacttggaa ttttccttaa atcttaacat
     2821 ttcttttttt tattaattat tctgcacgat catcaactgg tttttgaccg gctggccgtt
     2881 tgctgtcagc ctggcaacat aagtacccga aggtaaacgg tttgaccggg cgacctggca
     2941 attctgacgt ccgttcagcg attcccagga ggtttcactg atcttcttgc cggacagatc
     3001 aaaaaattcg agccgggcgt ctctcagcgt cttgtccgtt tcaacgaaca cggtattatt
     3061 ctgaatgagg gtcgggtaga tcttgattcc cctgatctca ttgttgttca cctgtacgac
     3121 cggtgaaata ttggaataat tatcttttcc gatcaccttg atccggtagt aagagattcc
     3181 ctgcagcgga tctgaatcgg taaaatgata gctcagctga taatcatggg tagagatcgt
     3241 ggtctgcgac ccgatacggc cgatctgcgt aaaatcctgg ccgtttccgc ttctttccat
     3301 ttcgtaatga tcgataccgc tttccatcat cgtcttccag cctaaatcaa cctcgtgatt
     3361 tgatttcaga ttaccttcaa atccggtcag cgtaaccggc aggatgctcc cgccgctcat
     3421 cgaaaagctt acccggttgc ttacggcatt ctcataatat tcgagaacta cgtcatagct
     3481 gccactcagc gtagccgtgt acgtggttgt gttatatccc tggtcgctcc atttattgat
     3541 gacataggta ctgccaccgt tcaggcttaa ccggtaaccg tcatcggcgc ctaccgtgat
     3601 ggtatagaca ccgctggtca gcaccgtccg taaccggaac cgcacactga actgggtggt
     3661 tacgatggaa cagccggtgg ttgcaaaact ggccacatct ccgccgaacc cttcgtcaaa
     3721 attgggagat accgaactgc cttccgttat atacccttta taagagtcga aattcattcc
     3781 gctgtaaaca tatcctaacc atttggtacc ggtaccgtaa acggtctggg ttccagttgt
     3841 cgtacaatct tttgtaatgt tgaatgaaac gcggttgtca ccactgttct cataatattc
     3901 caacacgaga ttggtgcttc cgttcaggtg aacggtcagg gtagtgcttg tataactctg
     3961 gagaacccag ctgttgattg cccaggtagc tcctccgtca agactcaacc ggtaaccgtc
     4021 atccccacct accacaatgg tataatcgcc atccacaaaa ctctgggcaa gcttgtaccg
     4081 catgctgaat gtttctgtag tggtggaaca gccgtccagc gtattatagg ctgagttggt
     4141 tcctccaaag ctttcatcaa aattcgggct ggagctggaa ccttcattaa catatccttt
     4201 ataggtatcg aaatttattc cgtcaaatac atatccaatc cagacattat tagtaccgta
     4261 gacagccgga gcggatccaa cggggcaacc tgcgttaacc agggtgctga tcaggataag
     4321 aacgggaagg attaaagact ttttcataaa attcagatta ctccttctat ttaccagtgc
     4381 aatgccagga tttaaacaat tgattatcaa atttttatgg cacttttgga atttgggttc
     4441 ttcccaatcc gggaaggaat tacgaaattg agccggttaa atactaactt gtattgacat
     4501 attcctgcaa tatgcccagc cagacaaccg cttccgccag gatctattca atcgatatat
     4561 tgagaggggc ggttatgatc atcatggccc tggaccatgt ccgggatttt tttcatttca
     4621 ctgccgccac cgattccccg accaatctgg ccagcacgac acctgcctta tttttcaccc
     4681 gctggatcac ccatttttgt gcccctgttt ttgttttact ggccggtatt tccgcctccc
     4741 tgatgagccg gaagaggacc aggccccaac tgagcctttt tctgattaaa cggggcctat
     4801 ggctgatcgg ggtcgaggtc ctcctggtga cctttgcatg gaccttcaat ccgttttata
     4861 atgtttttat tctgcaggtg atctgggcca tcggcatcag catgatcatt ctgggaatct
     4921 ttatccggta ttcctcacgg caggtcattc tgctggccgg actcatcatt atctgctgtc
     4981 ataacctgct cgattttccg gaggccgccg gaaagggaaa tatcggtttc ttctggaacc
     5041 tggcgcacca tgctgctttt accgtttacc ctgtttttcc aaaccatgag gttatcattg
     5101 tgtatgcttt cctgccctgg accggtatta tgatgacggg ctttgggatc gggaaaatat
     5161 tcagtaatga ggtccgttcc ggcctgagga gaagaatttt attcagttcc gggtttgccc
     5221 tgctgttgct tttttttatc ctccggttta taaatgcata cggagatccg gtggcctggt
     5281 cggttcaacg gacgccggtg ttcaccctgt tgtcgttttt aaatgtgacg aaataccctc
     5341 cttccctgga ttatatcgga ataaccatcg gaaccgctct gatcgttttg ggtttgctgg
     5401 aaaaggtatc cggaaaatca ttgaagtttc tgatcacgtt cggcagggtt cctttcttct
     5461 actatgttgt ccatttatat ttgattcacc tgaccgtctt cgtctttttt attctggaga
     5521 aatttccttc gaaagatatt gcagcccaaa gcctgccgtt ttatttcagg ccggaccggt
     5581 tcggatttaa tttatggatc gtctatggaa tttgggctgc catcatcccg atgatctatc
     5641 cgctgtgcag atggtacaac cggtacaaaa gcacccatta tcactggtgg ctgagttatc
     5701 tgtgaatcat cccctgagca tgtttttgat ctcattcagt ttgagcaggg cttccaccgg
     5761 tgtcagccgg tttatatcca gttcggataa tgatttccgg atctcttcga agagctgggt
     5821 atgggcatca aaaatggaaa gctgcagccg cggtgcggct atttttttta aaccctcctg
     5881 taaacctgac ccatctccgc tccggctctc ttccagttgt ttcaggatct cgtttgcccg
     5941 tccgatcagg tcggccggca ttcccgccat cctcgccaca tggatgccga aactatgcgt
     6001 gctacccccc ggtgccagtt tgcggaggaa aataattttg tttccgtgtt cccggctggt
     6061 gatatgaaaa ttttttatcc cgggaaaccg gtcttccagt tcattgagtt catgatagtg
     6121 cgtggcgaat aatgttttag gtttgaaggg tgaccggtgc aggtattccg cgatgctcca
     6181 ggcgatggag atgccatcgt aagtagatgt gccccggcca atttcatcga gaatgatcag
     6241 gctgcccggc gtaatattat ttaggatgct cgctgtttcg ttcatctcca cgagaaaagt
     6301 ggattcaccg gcactgaggt tgtcggatgc gcccacgcgc gtaaatatct tatcggtaag
     6361 cggaatcctg gcgcgggaag ccggtacaaa actgcccata tgcgccatca gggtaatcag
     6421 cgccgtttga cgaagcacgg cacttttacc gctcatgttg ggaccggtca ggataatgat
     6481 ctgttgggtt tccggatcca gaaagagatc atttggaata tattcttccc ccggaggcag
     6541 attcttttca atcacgggat ggcggctgtc gcgcaactcc agttccatgc ccgtatgaag
     6601 ctcgggtttt ttata
//
LOCUS       DASYCU010000477         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2275576, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000477 DASYCU010000000
VERSION     DASYCU010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275576"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGM24_09645"
     CDS             <1..381
                     /locus_tag="VGM24_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEY4155683.1"
                     /translation="NDELFRKILRGLNQAFYHKTVGAQDIEQYINRVSKMDFTPVFNQ
                     YLRSVQVPEFEYEQTPQQLRYRWTNCNPDFNLPLKVYLNTEVWLRPTTQWKNRKLDQK
                     ADTLRVDKNFYIRVKNSSGAESIP"
     gene            427..1947
                     /locus_tag="VGM24_09650"
     CDS             427..1947
                     /locus_tag="VGM24_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEY4155684.1"
                     /translation="MSTIRRQSILSSGIIYIGFALGLLNTYLFTREGGFTQDEYGLYG
                     TFIAIANIINSFANLGMQAYIYKFYPYYHDNLEVRDNDMMAWALTTSLLGFLMVMLAG
                     YIFKDLVIRKFGAHSADLVIYYSWIFPLGFGLTMYSLLEAYAWQLKKSVLTTFLREFL
                     FRFFTTALIVLVFTRVIHDFDLFIKLYALTYILLAVILLFLLIRSRQIHLTLRISRVT
                     RKFFKKIVVLTSFMWSGSLIYYIANSFDLIVIAAVLPNGLAWAGIFTLAQNGASLIQA
                     PQRGIIAASIGPLSRAWKDKDYGRIRLIYQRSSINQLLFACGIFVLIWINFTDGVRTF
                     HMQSNYLQAQNVFFFLGLIRVVDMGTGLNSQIIGTSAFWRFEFITGIIILAISLPLNY
                     ILAKRMGVVGPAIATLLAAGIYNTIRYFFLLRKFNMQPFNIRTLFTIGLAILGYYFTH
                     LLFSEMTGIPAMILRTAVFLLIYTGGAFLLRLSPDILPIWNTLKKKIPIPYLRSRK"
     gene            1992..>2365
                     /locus_tag="VGM24_09655"
     CDS             1992..>2365
                     /locus_tag="VGM24_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY4155685.1"
                     /translation="MQTLYSLEAQEDPDPGDAQKILRKHFEHSRQLFVYLVYFLTEIA
                     RYAEKDSARRSSKHLPTELDKNVNIKLAGNQLLWNILEQESFQKALKQEKSEFIDNAE
                     LVRRLYLELRESQPYKKYIEEN"
BASE COUNT          592 a          623 c          520 g          630 t
ORIGIN      
        1 aacgatgagc tgttccggaa aatcctgcgc gggctgaacc aggcttttta ccataaaacc
       61 gtgggggcgc aggatatcga gcaatatatc aaccgggttt ccaaaatgga tttcacaccg
      121 gtattcaacc agtacctgag aagtgttcag gttccggaat ttgagtacga acaaaccccg
      181 caacagctcc ggtaccgctg gaccaactgc aacccggact tcaatctgcc tctgaaagtt
      241 tatctgaata cggaggtatg gctccggccc accacccaat ggaaaaacag gaaactggat
      301 cagaaagcag atacgctccg ggtggataaa aatttttaca tccgcgtcaa aaacagttcc
      361 ggcgcggaat caatcccctg atttgaatca aaatccttca tctttgcagc gcaactttat
      421 tggcatatga gtacaatccg gcggcaaagc attctttcat ccggcattat ctatattggc
      481 tttgcgctgg gactgctcaa tacctacctg ttcacccggg aaggcgggtt cacccaggac
      541 gaatacggac tctacggcac ctttatcgcc attgccaaca tcatcaattc ctttgcgaat
      601 ctggggatgc aggcgtatat ctataaattc tatccgtact atcacgacaa tctggaagtg
      661 agggataacg acatgatggc ctgggccctc accacttccc tgctgggctt tctgatggtt
      721 atgctggcgg gatatatatt taaggatctc gtgatccgga aattcggcgc tcattcagcc
      781 gacctcgtca tatactattc ctggatattt cccctggggt ttggattgac catgtattcc
      841 ctgctggaag cgtatgcctg gcaactaaaa aaatcggtgc ttaccacctt tctgcgggaa
      901 ttcctgtttc gcttcttcac caccgcactc attgtcctgg tgtttacccg ggttatccat
      961 gatttcgacc tgtttattaa actttatgcc ctcacctata ttctgctcgc ggttatcctt
     1021 cttttcctgc tgatccgttc caggcaaatc catctaaccc tccggatcag ccgcgttacc
     1081 cggaagttct ttaaaaaaat tgtggtgctg acctccttta tgtggagcgg aagcctgatc
     1141 tattatattg caaactcctt cgacctgatc gtcattgccg ctgtgctgcc caatggattg
     1201 gcctgggcgg gcatttttac cctggcgcag aatggagcca gcctgatcca ggcgccccag
     1261 cgcggaatta ttgcggcgtc catcggacca ttatccaggg cctggaagga taaggattac
     1321 gggaggatcc ggctgatcta ccagagatcc tccatcaacc agctcctttt tgcctgtgga
     1381 attttcgttc tgatctggat caattttacg gatggcgtgc gcacgtttca catgcaatcc
     1441 aattacctgc aggctcaaaa tgtttttttc ttcctgggcc tgatccgggt ggtcgatatg
     1501 ggaaccgggt taaactcgca gattatcgga acctccgctt tctggcggtt tgaattcatc
     1561 accggcatca tcatcctggc catatcgctt ccgctcaatt atattctcgc taaaagaatg
     1621 ggcgttgtgg gaccggccat tgccacgctg ctcgcagcag gcatttacaa taccatccgt
     1681 tattttttcc tgctccggaa atttaatatg cagccgttta atatccggac gcttttcacg
     1741 atcgggctgg ccatcctggg ttattatttc acccatttat tattcagtga aatgaccggg
     1801 attccggcca tgatactcag aacagcggtt ttcctgctta tatataccgg cggagccttc
     1861 ctgctccggt tatcgccgga cattctcccc atctggaata ctttaaaaaa gaaaatcccg
     1921 attccctatc ttcgcagccg caaataagtt cttactacat gatcacgaga agaaatatca
     1981 gggttaaaat catgcaaacc ctatactccc tggaagctca ggaagatccg gacccgggcg
     2041 atgcacaaaa gatcttgcga aaacatttcg aacactccag gcaacttttc gtttacctcg
     2101 tttattttct gactgaaatt gcccgctatg cagaaaagga ctctgcccgc cggtcttcca
     2161 aacacctgcc gacggaactc gacaaaaatg taaatatcaa actggcaggc aatcagctgc
     2221 tctggaatat tctcgaacag gaatcctttc agaaggcgct gaaacaggaa aaatccgaat
     2281 tcatcgacaa cgcggaactg gtgcgcagac tctatctgga actgcgcgaa tcgcaaccct
     2341 ataaaaaata tattgaagaa aatga
//
LOCUS       DASYCU010000478         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4161485, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000478 DASYCU010000000
VERSION     DASYCU010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4161485"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VGM24_09660"
     CDS             complement(<1..1314)
                     /locus_tag="VGM24_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY4155686.1"
                     /translation="MKNFLLRICFCGLLFGLSLPRIRASDSLRIHLIPQPVNLSERPG
                     EFMLDQKVPLIVEASGQTMNETAAWFTDQLTTVSGLTIQQKKSGKKGIHLKLISGSED
                     NRIGDEGYILSVTPSAITLSAKKPAGIFYGMQTLLQLLPANARMIAGGNSKRLAIPCA
                     EITDYPRFGWRGIMLDVSRHFFTKAEVEKYIDEIVRFKYNVLHLHLSDDQGWRIEIKS
                     LPRLTAMGAWRVKRVGQWGQFLPALANEPATDGGFYTQDDMREIIRYAQMRNVTILPE
                     IDVPAHSLALITAYPNLSCTQLQYSVNAGFHSPVRDDNALCIGNDSVFLMLDKIFTEL
                     AALFPCEYIHVGGDEAYKGFWADCPKCKKRMADEHLKNVDQLQSYFVKRVEKILKSKG
                     KKLIGWDEILEGGLAPEATVMSWRGMKGGIEAAKMNHHVVMTPWDY"
     gene            complement(1423..2442)
                     /locus_tag="VGM24_09665"
     CDS             complement(1423..2442)
                     /locus_tag="VGM24_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4155687.1"
                     /translation="MANTIPTLKEIAERLNLSISTVSRALHHHPSIGLRTSMRVQQLA
                     NELNYEPNSAAIYFKQKRTSLVGVIVPRLSQEFFASIVNGIEDIAYKHKYTVVLCQSR
                     DEEEREIQLVEAMKSNRVDGLLVSISKKTKNYAHFESLKKYNIPVVFFDRIPDMPDIH
                     FIACQLKSGIEEAISFLSGRKHQSIALINGPENLLASQERLEGYKLALKTHKIKFDPS
                     RIASSDLSKEGTEQAMKKLLSSKKKPTAIIAFNDYVAMDAIQFARKSNIRMNKDLCFV
                     SFANEAITNYLEHPPIASIEQFPYQQGEKAMEILLQLMEKKNDEPSISVIHNILIQSQ
                     LVIHD"
     gene            2589..>3295
                     /locus_tag="VGM24_09670"
     CDS             2589..>3295
                     /locus_tag="VGM24_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4155688.1"
                     /translation="MSSLFKDQVAIITGAASGIGKAIAKKLSEKGIYLALFDLKQEAL
                     LELKEQIGGNCELYGLDITDEDKVRKTIDQVGLHFGRIDILVNSAGITGQTNTKSHEV
                     PSDNLRAVFEINFIGSYLTSRYVLPWMLKRSYGRILHIASIAGKEGNAGMLAYSASKA
                     AVIGMTKVQGKEYAQTGITVNALAPAVIRTAMVEAMPEEQVNYMTEKIPMKRCGTLEE
                     AADCALFIVSPENSFTTG"
BASE COUNT          813 a          767 c          830 g          885 t
ORIGIN      
        1 ataatcccag ggcgtcatca cgacgtggtg attcattttt gctgcttcaa ttccgccctt
       61 cattcctctc cagctcatga cggttgcttc cggggcaagt ccgccctcca gaatttcatc
      121 ccagccgatc agttttttgc ccttgctctt caatattttt tccactcttt ttacaaaata
      181 gctttgcaat tgatccacgt ttttcaggtg ttcatcagcc atccgtttct tacacttcgg
      241 acaatcggcc caaaagcctt tgtatgcttc atctccgccg acatgtatgt attcacaggg
      301 aaagagggcc gccagttcgg taaatatctt atccagcatg aggaagacgg aatcgtttcc
      361 gatgcataat gcattgtcgt cccggacggg gctgtggaat cccgcattga cgctatattg
      421 cagttgcgtg caggaaaggt tcggataagc ggttatcagg gcgagactgt gtgccggcac
      481 atcgatctcc ggcaggatgg tcacattccg catttgcgca tatctgatga tttcccgcat
      541 atcatcctgg gtataaaatc cgccatccgt agcgggctca ttcgccagtg ccggaaggaa
      601 ttggccccac tgaccgacac gtttcacgcg ccaggccccc atggccgtca accggggcag
      661 gctcttgatt tcgatccgcc atccctggtc atcgctcaga tgcaggtgga gcacattata
      721 tttgaaccgg acgatttcat cgatatattt ttcaacttcc gccttggtga aaaaatggcg
      781 gcttacatcc agcatgatcc cgcgccagcc gaagcgggga tagtccgtaa tttctgcgca
      841 gggaatcgcc agtcgtttgg aattaccacc ggcaatcatt ctggcgtttg cgggcaataa
      901 ctgcagcagg gtctgcatgc cgtaaaaaat cccggcgggt ttttttgcgc tcagggttat
      961 ggccgaaggg gtgacagaca aaatataccc ctcgtcgccg atccggttat cctcgctgcc
     1021 gctgatcagt ttcaggtgaa tgcctttctt tcccgatttt ttctgctgaa tggttaaacc
     1081 ggatacagta gtcagctgat ccgtaaacca ggctgccgtt tcattcatcg tttgcccgga
     1141 tgcctccacg atcagcggca ccttttgatc aagcatgaat tcgcctggac gttcgctcag
     1201 attcaccggt tggggaataa gatgaatacg caggctatcg gaagcccgta tccggggaag
     1261 actaagaccg aacagcagac cgcaaaaaca aatccggaga aggaaatttt tcatctttga
     1321 aattttgaat gccgaattta aacctttttt agtttcgtcg gctggccggc gtggaagaaa
     1381 ccggtggacg gatcctgccg ccgcccggca tgaacccttc cattaatcgt gaatgaccag
     1441 ttgggactgg atcaatatat tgtgaataac tgaaatggaa ggctcatcat ttttcttctc
     1501 catgagttgt aaaaggattt ccatggcttt ctcgccctgc tgataaggga attgttcgat
     1561 ggaagcgatc ggtggatgtt cgaggtaatt ggtgatggcc tcgttggcaa aactcacaaa
     1621 acagagatcc ttgttcatcc tgatatttga tttcctggca aactgaatgg catccatggc
     1681 gacataatcg ttaaacgcga taatggctgt cggctttttt tttgaactca ataatttttt
     1741 cattgcctgc tctgtgcctt ctttggaaag atcggaactg gcaatccggg aaggatcaaa
     1801 ttttatctta tgtgttttca gggccagttt atagccctcc agccgctcct ggctggccag
     1861 gagattttcc ggtccgttga tcagggcgat agactggtgt ttacgtccgg acaggaaact
     1921 gatggcttct tctatgccgg atttcagctg gcaggctata aaatggatat ccggcatatc
     1981 gggaatccgg tcaaaaaaca cgacgggaat attgtacttt ttcagtgatt cgaaatgggc
     2041 gtaatttttt gttttttttg aaatggagac cagcaggcca tccacccggt ttgatttcat
     2101 ggcttccacc aattggattt ccctttcttc ttcgtcccgg gactggcaga ggacgacggt
     2161 gtatttgtgc ttataggcaa tatcttcaat gccattgaca atggaggcga aaaattcctg
     2221 ggacaggcgg ggaacgatca cgccgaccag ggaagttctt ttttgtttaa aataaatcgc
     2281 ggcactgttg ggttcataat tcagttcatt ggccaactgc tgcacccgca tgctggttct
     2341 cagtccgatg ctgggatgat ggtgcagggc cctcgaaacg gtggatatgg aaaggtttaa
     2401 ccgttctgca atttccttaa gtgtgggaat ggtattggcc atacgcgggt cttgcgggta
     2461 aagatagaat acaaacgatt gcgtaaattt aaattacgca atcgtttgtg cgaaatacgg
     2521 aaagaatccg ggtgtaattt taaggatctc ccgggtaaga acgaccttca taacttaaaa
     2581 cacgaagcat gagctcgcta tttaaagatc aggtggctat tataaccgga gcggcctccg
     2641 gaatcgggaa agctattgca aaaaaattat cggagaaggg aatatatctg gccttgttcg
     2701 atctgaaaca ggaggcgtta ttggaattaa aagaacagat cggcggcaac tgtgagctgt
     2761 atggcctgga cattacggat gaggacaaag tgcggaagac cattgaccag gtgggtttac
     2821 atttcggacg aattgatatt ttggtcaata gcgccggtat aacgggacag accaatacaa
     2881 aaagtcatga agtcccttcc gataacctgc gcgctgtgtt tgaaattaat tttatcggct
     2941 cctatttaac ctccaggtat gttttaccct ggatgctaaa aaggagttac ggacgcattt
     3001 tacatatcgc ctctattgcc ggtaaggaag gaaacgccgg catgctggct tattccgcat
     3061 caaaagccgc cgtgatcggc atgaccaaag tgcagggcaa ggaatatgcc cagaccggta
     3121 tcaccgtgaa tgcgctggct cccgcggtga tccggacggc catggtagag gccatgccgg
     3181 aggagcaggt aaattatatg accgaaaaaa taccgatgaa aagatgtggc acgctggaag
     3241 aagcggccga ttgcgcattg tttatcgttt cacccgaaaa cagctttact accgg
//
LOCUS       DASYCU010000479         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2713378, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000479 DASYCU010000000
VERSION     DASYCU010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713378"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VGM24_09675"
     CDS             <1..1288
                     /locus_tag="VGM24_09675"
                     /inference="COORDINATES: protein motif:HMM:NF045135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155689.1"
                     /translation="KLASVVNAVGYHYVYGHLPRMDNEENPVPASAKKLGVSLWASED
                     WSMYNGSWNNAHILAGILNKMYIRDRITAMEIWCPFDGYYDNTGDYHSTGLFQADQPW
                     SGHYRILPAVWAVAHFTQFTEPGWKYINSGCNYFDKPSGGNYTTLYNPATGDFTTIVY
                     ADSATRKKIVIRPEGRLSKPFLYVWKSDAESQFQLIKKIKPDSHNRFEVDIDPESIYT
                     ISTTTGQQKGSVKMQMPPAPFPIPYKEDFSAVPVRKNPRFFADIEGAFEVAADPVTKN
                     KFLCQQITDPPINWTYSGGFKPLGPLTELGDVAWKDYALSVDVQIPGKGYAQIIARTG
                     NLREYTEGYVLKLYHNGYWELLLNSHLILAAGQIGMKAGAWHQLELKCVGDSIEAQID
                     GQLLAGVRNNLVKSGMASLGSSWDRVKFDNLSIQKL"
BASE COUNT          417 a          350 c          375 g          359 t
ORIGIN      
        1 gaaactggcc tcggtggtca atgctgtcgg ctaccattat gtatacggac acctgcccag
       61 gatggataat gaagagaatc cggttcccgc gtctgcaaaa aaactcggtg tctcgctttg
      121 ggcttcggaa gactggtcca tgtacaacgg aagctggaac aatgcgcata tacttgcggg
      181 cattctcaac aagatgtata tccgggaccg gatcacggcg atggaaatct ggtgtccctt
      241 cgacgggtat tatgataata ccggggatta tcattccacc gggttatttc aggccgacca
      301 gccctggtcg ggccattacc ggatcttacc cgccgtttgg gctgtagccc atttcaccca
      361 gtttaccgaa ccgggctgga agtatatcaa tagcggctgt aattattttg ataaaccgtc
      421 cggtggtaat tataccacat tgtacaatcc ggcaaccgga gatttcacta cgatcgtgta
      481 tgcggattcg gccacccgga aaaaaatagt gatccgcccg gagggcaggc tctccaaacc
      541 ttttctatat gtttggaaat ccgatgcgga aagccagttt caactgataa agaagatcaa
      601 accggacagc cataaccgct ttgaggtgga tattgatccg gaaagcatat acactatatc
      661 cactacaacg ggtcagcaaa aaggatccgt taaaatgcag atgccgccgg ctccttttcc
      721 cattccgtac aaggaagatt tctcagctgt gcccgtccgc aaaaatcccc gcttttttgc
      781 agatattgaa ggcgcttttg aagtggctgc ggatcccgtt acaaaaaata aattcctgtg
      841 ccagcagatc accgacccgc cgattaactg gacctattcc ggaggtttca aaccgctcgg
      901 ccctttgact gaactcgggg atgtcgcctg gaaggactat gcattgtcgg tggatgttca
      961 gattccgggg aaaggatatg cccagatcat cgccagaaca ggtaacctcc gggaatacac
     1021 ggaaggctat gtgctgaaac tctatcataa cgggtactgg gaactgttgt taaacagtca
     1081 tctgatcctc gccgcaggac agattgggat gaaagccggc gcctggcatc aattggaact
     1141 gaaatgcgtg ggtgattcca tagaagcgca gatagatggg cagctgctgg ccggcgtacg
     1201 gaataatctg gtaaaatccg gtatggccag tttgggaagt tcctgggacc gcgtaaaatt
     1261 cgataatctt tcgattcaaa aattatagac gacatattta gaaaatgctg atcaatccga
     1321 aacagccaat cacttaaaaa atacagatgg aaaaaaaggc agcaactcaa gtcattaaga
     1381 aaatgtggga cacggacgat gaattgtttc accttgttcg aaaagaactt tatacggcag
     1441 tagttggaga tatcatggat aagctggggt atctgaacca gtttctgcct ccccggatac
     1501 a
//
LOCUS       DASYCU010000480         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3583185, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000480 DASYCU010000000
VERSION     DASYCU010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583185"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VGM24_09680"
     CDS             complement(<1..1261)
                     /locus_tag="VGM24_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY4155690.1"
                     /translation="MQDYIQGLNEAQKEAVMHIDGPLMIVAGAGSGKTKVLTTRIAHL
                     MKSGVDAFNILALTFTNKAAREMKERVEHILGNNEARNLYIGTFHSVFARILRAEAPK
                     IGYPRHFTIYDTDDAKAVIKTVVNELNLDDKHYKPGIVYGRISQAKNGLISPAEYATD
                     YNIQQEDLRSNRPAIAQIYEAYAKRCFKNGSMDFDDLLLKFYELLKHFPESLHKYQHK
                     FKYILIDEFQDTNPAQYEIIKLLGAVYENVCVVGDDAQSIYSFRGATIENILQFQKDY
                     DDVKVVKLEQNYRSTQSILSVANEIIKRNKGQIPKNLWTENVEGEKIRLVRTMTDNDE
                     GKFVADTIQEQKLRNHFNNKDFAILYRTNSQSRAFEESLRRMGIPYTIYGGISFYQRK
                     EIKDFISYLRVIINPNDEESLKRIINYP"
     gene            complement(1331..1807)
                     /locus_tag="VGM24_09685"
     CDS             complement(1331..1807)
                     /locus_tag="VGM24_09685"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY4155691.1"
                     /translation="MVGKLMMHTKVKSFILIAGLFALLASLNAQTPHKGNAPSGKAGL
                     EGKWYLQAVLPSDTAAGKLPEIVFNTREGRFSGNTGCNSMQGSFQATDSSIRFNEPMI
                     TTKMMCVGYNEGAFLKNLIHTNTFKIQNGLLILLVDGTEISRWSRKITKPKKSFNT"
     gene            1822..3069
                     /locus_tag="VGM24_09690"
     CDS             1822..3069
                     /locus_tag="VGM24_09690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY4155692.1"
                     /translation="MPGFEFFGEEERKEVNDVLQTGILMRYGFDGPRKGIWKAKELEE
                     ALCKRLGARYAQLTSSGTSALTTALAALGVGAGDEVIMPSFTFVASFEAVLSVGAVPV
                     IVDINETLTLDPESVKKAIGPATKCIMPVHMCGSMADLDALRAICREKKHLLLLEDAC
                     QSIGASYKGKSLGTIGDAGALSFDFVKTITCAEGGAVLTNDKAIYTHCDGYSDHGHDH
                     LGMDRGAEPHPFLGYNYRISELHAAVGLAQIRKLDRFLAIQKKNHQYLQDSLSRIPGI
                     RFREIPDPAGDSHSFLSWFLPTAELTRAVIAEMKNRNILAGNFYWFEHNWHYIRKWDH
                     LKQAVTLGPLTEAQIKALKDLQTRDFSQSDAVMSRCVSTAIGLSWTEDQLKEKASGIA
                     SAVKQVLANVSPQAHYSNQANPA"
     gene            complement(3020..>3291)
                     /locus_tag="VGM24_09695"
     CDS             complement(3020..>3291)
                     /locus_tag="VGM24_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155693.1"
                     /translation="SLLFPGEMLKNKNPHFTPDSAFYPVSAYDLLFFSNKQEISGETS
                     VPANIYYCQSGFFCKREWELEKATHIPFRFRLGSLDYCNALEGKH"
BASE COUNT          788 a          831 c          838 g          834 t
ORIGIN      
        1 ccggataatt gataatccgt ttcagcgatt cctcgtcatt cgggtttatg atcacgcgca
       61 gataggaaat aaaatccttg atctctttgc gctggtaaaa actgatgccg ccataaatgg
      121 tatagggaat gcccatcctc cggaggcttt cttcaaaagc ccggctctgc gaattggtcc
      181 ggtaaagaat ggcaaaatcc ttattgttaa aatggttccg gagcttttgt tcctggatgg
      241 tatcggccac aaatttgcct tcgtcgttgt ccgtcatggt gcggaccagg cggatctttt
      301 ctccttccac attttccgtc cacaggtttt tcgggatctg tcccttattc cgtttaatga
      361 tctcgttggc gacgctgagg atgctttggg tgctgcggta attctgttcc agcttaacca
      421 ctttcacgtc gtcgtaatcc ttctggaact gcagtatatt ttcgatggtg gcgccgcgga
      481 aactgtaaat actctgggca tcgtcgccca ccacacaaac attttcatat acggctccca
      541 gtagtttgat gatctcgtac tgggccgggt tggtgtcctg gaactcatcg atcaggatgt
      601 atttgaattt gtgctggtat ttgtgcaggc tttccggaaa gtgtttcaac agctcataaa
      661 atttcaggag caggtcgtca aagtccatcg acccgttttt aaaacaacgt ttggcataag
      721 cctcatagat ctgggcaatg gccgggcggt tgctgcggag atcctcctgc tggatattat
      781 aatcggtggc atattccgcc ggggagatca acccgttttt cgcctgggag atccggccgt
      841 acacaatacc gggtttataa tgtttgtcgt cgagattaag ttcattcacc accgtcttta
      901 tcaccgcctt ggcatcgtcg gtatcgtaga tcgtgaaatg ccggggatac ccgattttgg
      961 gggcttcggc acggaggatg cgtgcaaaaa cactgtgaaa agtgccgata tagagattgc
     1021 gtgcttcgtt attgcccagg atatgctcca cccgttcctt catctcgcgc gcggctttat
     1081 tggtgaaggt gagagcgaga atattaaatg catccactcc gcttttcatc aggtgggcga
     1141 tacgggtggt gagcactttt gttttaccgc tgccggcacc ggcaacaatc atcaaagggc
     1201 cgtcgatatg cattaccgcc tctttctgcg cttcatttaa tccctgaata tagtcctgca
     1261 tggctgcaat ttacgaagac tgattttgtc cccggcagcg gaaagtgaaa agaggataag
     1321 tattaaaccc tcaggtattg aatgattttt tgggtttggt tattttccgg ctccagcggg
     1381 agatctcggt gccgtccacc agcagaatga gcagtccatt ctgaatctta aaggtattgg
     1441 tgtggatcag gttctttaaa aaagcccctt cgttataccc gacgcacatc atttttgtgg
     1501 tgatcatagg ctcattgaaa cggatgctgg aatccgtagc ctgaaaactg ccctgcatag
     1561 aattacagcc ggtattgccg gaaaaccgcc cttcccgggt attgaaaaca atttcaggga
     1621 gttttccggc agcggtatcg ctgggcagta ccgcctgcaa ataccatttc ccttctaacc
     1681 ccgcttttcc ggaaggagca ttccctttgt gcggggtctg cgcattcagg gaagccagca
     1741 gcgcaaatag cccggcaatg agtataaaag attttacctt tgtgtgcatc attaatttcc
     1801 caaccataat catacctaaa tatgcccgga tttgaatttt ttggtgaaga agagagaaag
     1861 gaagtgaacg atgttctgca gaccggaatc ctgatgcgtt atggtttcga cggtccccgc
     1921 aaaggaatat ggaaagcaaa ggagctggaa gaggcgcttt gtaaacggct gggtgcccgt
     1981 tatgcgcaac tcaccagtag tggcacttcg gcactcacga ccgccctggc cgccctggga
     2041 gtcggagccg gggatgaagt gatcatgccc tctttcacct tcgtagccag ttttgaagca
     2101 gtgctcagcg tgggcgccgt gccggttatc gtggatatca atgaaacact cacccttgat
     2161 ccggagtccg ttaaaaaagc catcggtccg gcgaccaaat gcatcatgcc cgttcacatg
     2221 tgcggcagca tggcggacct ggatgcgctc cgggccatat gccgggaaaa gaagcatctc
     2281 ctcctgctgg aagatgcctg tcagagcatc ggcgcttcct acaagggaaa atctttggga
     2341 accatcggcg atgccggcgc cctttctttc gacttcgtaa agaccatcac ctgcgcggaa
     2401 ggcggcgccg tattgaccaa tgacaaagcc atttataccc actgcgacgg gtattcagac
     2461 catggtcatg accacctggg tatggaccgg ggcgccgaac ctcacccctt tctggggtat
     2521 aattaccgga tctccgaatt gcatgccgcc gtcggcctgg cccagatccg caaactcgac
     2581 cgtttcctgg ccatccagaa gaaaaaccac caatacctgc aggacagcct gtcccggatc
     2641 ccgggcatcc ggttccgtga aattccggat cctgcgggag acagccacag cttcctaagc
     2701 tggttcctgc caactgccga gctgacgcga gccgtaatag ctgaaatgaa aaaccggaat
     2761 atcctggcgg gaaatttcta ttggtttgaa cacaactggc attatatcag aaaatgggat
     2821 cacctgaaac aggccgtaac gctgggcccg ttgacggaag cccagataaa ggcactcaaa
     2881 gacctgcaga cccgggattt cagccagagc gatgccgtca tgagccgttg tgtttccacc
     2941 gccatcggac tttcctggac cgaagatcag ctaaaggaaa aggcatccgg aatcgcttcg
     3001 gcggtcaaac aggtgctcgc taatgtttcc cctcaagcgc attacagtaa tcaagcgaac
     3061 ccagcctgaa ccggaaggga atatgcgtgg ctttctccag ctcccattcc cgcttacaaa
     3121 aaaaaccgga ctggcaataa tagatattgg ccggaacgga ggtttccccg gatatttcct
     3181 gcttattgga gaaaaagaga agatcgtatg ctgaaactgg ataaaatgcg gaatccggcg
     3241 taaaatgggg gtttttattt tttaacattt cccccggaaa aagcagactg t
//
LOCUS       DASYCU010000481         4151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1845211, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000481 DASYCU010000000
VERSION     DASYCU010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845211"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VGM24_09700"
     CDS             <1..332
                     /locus_tag="VGM24_09700"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEY4155694.1"
                     /translation="TGTAAGLPQLSGQRIHPYTDLLLHDMGEGLADHRPDYLASGSEW
                     RTQPLWGIGLVQKTTGTAYYLHDGRARTVEEAILWHGGEAQKSRGAFLQLSSAERNQL
                     LKFLSSL"
     gene            380..1441
                     /locus_tag="VGM24_09705"
     CDS             380..1441
                     /locus_tag="VGM24_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY4155695.1"
                     /translation="MKTGVVGYGVAAQFMHLPFIITNPDYQLLSILQRHGDSAAEKYP
                     AVQVVRSLDEMLADPSLELVVVTTPNDTHFDYTSRALRAGKHVVLEKPFTITSEEAKE
                     LIVIARESGKVLSVFQNRRYVSDFLTIRQLLNENKLGEVVEFEAHYDRYRPEQRPNAW
                     READAPGSGILYDLGAHLIDQAFCLFGLPESVTADLRIQRPHARTTDYFDLWMDYGFT
                     KVILKAGMLVREPGPRYMIHGTRGSFIKYGEDPQEALLRQGVLPNIRDWGKEPETFYG
                     LLHTEIGGETIREKYPSLPGNYGGYYADLYDTLVHGKPLKVKPEHGYNTIRLIELATE
                     SSRKNKTVDCTDLMDVPYP"
     gene            complement(1438..2430)
                     /locus_tag="VGM24_09710"
     CDS             complement(1438..2430)
                     /locus_tag="VGM24_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HEY4155696.1"
                     /translation="MNNSVSPIKYDGIFSGLPEHFEREALSVFHLQYAQNELYRQFAS
                     SLRKEPERVHALEDIPFLPISFFKTRTVKTGQFIPEIIFESSGTAGQQRSHHELRSVS
                     LYRESFSRGFELFYGPVSDYCIIGLLPAYLERSGSSLVFMVDELIRQSRHPQSGFYLD
                     DFGRLHDQLKLLEKNQQKTLLIGVSFALLDFAEKFPLPLQFTQIMETGGMKGRREELT
                     RARLHEILRQAFGLREIHSEYGMTELLSQAYSKGNGIYHPVPWMKAMIRKEDDPLDLQ
                     KTGEGLLNIIDLANRDSCAFIATDDLGKVYPDGSFEVLGRMDNSDLRGCSLLVV"
     gene            2537..2983
                     /locus_tag="VGM24_09715"
     CDS             2537..2983
                     /locus_tag="VGM24_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155697.1"
                     /translation="MKPRIPLFFCLALFALGCSKDKFEVNPSLRVKSFTGVVPVGGTF
                     NAVLEYSQKKGKIDGDTLIVYRHRYNQKPLPIGTPTVDSFYTILSGSGATIPDANSAE
                     FSLDLDYTNIHIDNGENDTIDFQFALIDLAGRSSDTVRTGKIVVLQ"
     gene            2995..3843
                     /locus_tag="VGM24_09720"
     CDS             2995..3843
                     /locus_tag="VGM24_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155698.1"
                     /translation="MDSLRKIFNFFLFSSIYISLCSVLMTWQTCHILKLPVSYDFFWF
                     VFFASICSYNFHWFLTPQSHKSPDRVKWTHEHKNAHLLFYFAGVIGSIVFFIKLRDHW
                     FALLFATLITFLYSAPKIPLPYFRFLKQVAIGKTTFLTTVWTYVTAIMPVFISSENFH
                     FNHQMKLFAASRFFLIYGICILFDYRDRDDDRDEGIRSMITYLDEKGVNRLFSFSLIA
                     FFVFTLGLLFTGLSLASVIVQLIPGLILAILYPYAKRNFSDYLYYFVLDGLMMLSGLL
                     MLILPI"
     gene            3870..>4151
                     /locus_tag="VGM24_09725"
     CDS             3870..>4151
                     /locus_tag="VGM24_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEY4155699.1"
                     /translation="MAKGSGTAVSKKSILTDNSYTFLKNYLNTPSPVGFESEGQKKWI
                     AYLKPYIDSYFTDPYGSAVGVINAKSDFKVVIEAHADEISWFVNYVNPEG"
BASE COUNT         1035 a         1063 c          993 g         1060 t
ORIGIN      
        1 tgaccggtac ggcagccggc cttccgcaac tcagcggtca gcggatccat ccgtatacgg
       61 acctgctcct gcacgatatg ggagaaggcc tggcggacca tcgcccggat tatcttgcca
      121 gtggcagcga atggcgcact cagcccttat ggggcatcgg gctggttcag aaaacaaccg
      181 gcacggcgta ctacctgcat gatggccgag cccggacggt ggaagaagcc atcctctggc
      241 atggcggcga agcccaaaaa tcccgcggcg cttttttgca gttaagctcc gcagaaagaa
      301 accagctgtt gaaatttttg agttcgctct aatcattctg acgcactccg gcttaatttc
      361 gcccaacaaa aataaattta tgaagacagg agtggtcgga tacggggtgg ccgcgcagtt
      421 catgcacctt ccatttatta ttaccaatcc ggattaccag ctcctgagta ttttacaaag
      481 gcacggagac agcgccgccg aaaaatatcc ggccgttcag gtggtcagaa gcctggatga
      541 aatgctcgcg gatccttcat tggaactggt tgtggtgacc acgcccaacg acacgcattt
      601 tgattataca tcccgcgcgc tccgggccgg caagcatgtc gtgctggaaa aacccttcac
      661 gatcaccagt gaggaagcaa aagaactgat cgtcattgcc agggaatccg gtaaggtgct
      721 cagcgtattc cagaacagaa gatatgtaag cgattttttg accatccggc agctgctgaa
      781 tgaaaataaa ctcggcgagg tggttgaatt cgaagcgcac tatgaccgct accggccgga
      841 gcaaaggccc aatgcgtggc gggaagccga tgcgcccggc agcggtattt tatatgatct
      901 cggcgcacac ctgatcgatc aggcattctg cctgttcggc ctgccggaat cggtgacggc
      961 ggacctgcgc attcaacggc cccatgcccg gacaacggat tactttgatc tctggatgga
     1021 ttatggattt accaaagtga ttttgaaagc gggtatgctt gtccgtgagc ccggcccgcg
     1081 gtatatgatc cacggaacca ggggttcttt cataaagtac ggggaggatc cgcaggaagc
     1141 cctgcttcgg cagggagttc ttcccaatat cagggactgg ggcaaagagc cggaaacatt
     1201 ttatggatta ctacatacgg aaattggggg cgagaccatc cgggagaaat atccttccct
     1261 gccgggaaat tacggaggct attatgcaga cctgtatgat accctcgtgc acggcaagcc
     1321 attgaaagta aaaccggaac acgggtataa taccatccgg ctgattgagc tggctacgga
     1381 aagcagccgg aaaaacaaaa cggtcgactg cacggacctg atggatgttc cttatcctta
     1441 aacgaccagc aggctgcatc cgcgcaggtc gctgttatcc atccggccca gcacttcgaa
     1501 tgatccgtcc gggtaaactt ttcccaggtc atccgtggcg atgaaagcgc aggaatcccg
     1561 gttggcgaga tcgatgatat tcaataggcc ctcgccggtt ttttgcagat ccagcggatc
     1621 atcttctttg cggatcatcg ctttcatcca gggaaccgga tgatagattc cattcccttt
     1681 tgaataagcc tgagacagga gctccgtcat tccatactcg gaatgaattt ccctaagccc
     1741 gaacgcctgc cggaggatct cgtgcaggcg ggcgcgggtt aattcctccc tgcgcccttt
     1801 cattcctccg gtttccatga tctgcgtgaa ttgcaagggc agggggaatt tttcagcaaa
     1861 atcaagcagg gcaaaactta ccccgatcag cagggtcttt tgttgatttt tctccagaag
     1921 cttcagctgg tcatgcagcc gcccgaaatc atccaggtaa aatccgctct gcggatgccg
     1981 gctctgccgg atcagttcat ccaccataaa aaccagggat gatccggacc tttccaggta
     2041 agccggcagc agaccgataa tacagtaatc ggaaacgggt ccgtaaaata attcgaaccc
     2101 ccggctgaag ctctcccggt acaaagagac cgatctcagt tcatgatgac tgcgttgctg
     2161 ccccgccgtg ccgctgctct caaaaatgat ctccggaata aactggccgg tcttaaccgt
     2221 tctggtcttg aaaaaggaga tgggcaaaaa ggggatgtct tccagggcat gaacccgttc
     2281 cggctcttta cgaagcgagc tggcaaactg gcggtaaagc tcattctgag cgtattgcaa
     2341 atgaaaaaca gacaaggctt cccgttcaaa atgttcgggc aacccggaaa atattccatc
     2401 gtatttaata ggagaaacag aattgttcat aatcaatttc cggaatacgg aataaagcca
     2461 aatgaatttt aaaaacaggt acaatactaa agcttttcgc gaaaatccgt taatttgcaa
     2521 cgttcaaaac atttgtatga aaccgaggat ccccttgttt ttttgccttg ccttatttgc
     2581 cctgggctgc agtaaggata aatttgaggt gaatccgagc ctgcgggtca agtcgttcac
     2641 cggagtggtg ccggtaggcg gaacgttcaa tgccgttctc gaatacagtc agaagaaggg
     2701 aaagatcgat ggagataccc tgatcgttta ccggcaccgg tataatcaga aaccgctccc
     2761 gatcggtacg cccaccgtgg attcttttta tactattctc agcgggtccg gagcaaccat
     2821 tccggatgcc aacagcgccg aattcagcct ggatctcgac tatacgaata ttcacataga
     2881 taacggggaa aatgatacca tcgattttca gtttgcactg atcgacctgg caggaagaag
     2941 cagcgatacc gtaagaacgg gtaaaattgt cgttctccaa taattacaaa caacttggat
     3001 tcgctacgga aaatctttaa cttttttctc ttcagcagta tctatatttc actttgctct
     3061 gtcctgatga cctggcagac ctgccacatc ctcaaacttc ccgtttccta cgattttttc
     3121 tggtttgttt tttttgccag catctgcagt tataattttc actggttcct gacgccccag
     3181 tcacacaaat cgccggaccg ggtgaaatgg acgcacgaac acaagaacgc ccacctcctg
     3241 ttttatttcg ccggcgtcat cggatctatt gtttttttca tcaaactgcg ggaccactgg
     3301 tttgccctgc tttttgccac cctgatcact tttctttatt ccgcccctaa aattccactc
     3361 ccgtatttcc ggttcctgaa acaggtggcg atcggaaaaa ccacttttct gaccactgta
     3421 tggacctatg tcaccgccat tatgccggtg ttcatttcca gtgagaattt tcattttaat
     3481 caccagatga aactttttgc agcgagtcgt ttttttctga tttacggcat ttgcatcctg
     3541 ttcgattacc gtgaccggga cgatgacaga gacgaaggca tccgcagcat gatcacttat
     3601 ctggatgaaa aaggggtaaa ccggcttttt tctttttccc tgatcgcttt ttttgttttc
     3661 acgctgggcc tgcttttcac cggcctttcc ctcgcatccg tgatcgttca gctgataccc
     3721 ggacttatcc tggctattct ttatccttat gcaaaacgca atttctccga ctacctgtac
     3781 tattttgtgc tggacggatt gatgatgctt tccggcctgc tgatgctgat actgccaatt
     3841 tgattatttt tggccttaaa ttatcattca tggccaaagg ctcgggcact gcagtttcca
     3901 aaaaatcaat actcaccgac aattcttata cttttcttaa aaattacctg aatacccctt
     3961 ctccagttgg ttttgaaagt gagggtcaga aaaaatggat cgcctatctc aaaccttata
     4021 ttgacagtta ttttaccgat ccttacggat ctgctgttgg cgtgatcaat gccaaatcgg
     4081 atttcaaggt cgtgatcgag gcccacgccg atgaaatcag ctggtttgta aactacgtaa
     4141 accccgaagg a
//
LOCUS       DASYCU010000482         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_104916, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000482 DASYCU010000000
VERSION     DASYCU010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104916"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..646)
                     /locus_tag="VGM24_09730"
     CDS             complement(47..646)
                     /locus_tag="VGM24_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HEY4155700.1"
                     /translation="MSNAKNILGLQLGTDPRWVDLASLSLEEILTDHAFCEQKAAITC
                     ISLIQKYSDRTRLVSDLAPVVTEEWGHFRQVLAELTKRGLKLGKQRKDEYVNHLISFQ
                     RKGGDFEDRFLDQLLTMAMIEARSCERFKRLSEGLKDEYLRNFYRRFMESEAGHYHLF
                     IDLAETYLGKEKVRKRWAEWLQYEAGIISKIDVRGDRIH"
     gene            753..1877
                     /locus_tag="VGM24_09735"
     CDS             753..1877
                     /locus_tag="VGM24_09735"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY4155701.1"
                     /translation="MNSDFLDRKLQERFSRNAYRRLQLAEGKHDFFSNDYLGISKNGL
                     IEAALEGTSFSPGSTGSRLLAGNYALMEETENRIAEFHQSPSALIFNSGYDANLGLLA
                     SVADRGDTILYDKLSHASIRDGIRLSLAEAFSFQHNSPGDLEKKLQKKKGNCFVVTES
                     VFSMDGDQAPLAELVPLCERFGAHLIVDEAHATGVIGGRGEGLVQLEGLQQRCFARVH
                     TFGKALGCHGAAVLGSEMLRNYLINFSRPFIYTTALPPSAVAAIRASYEIFPGLNPER
                     RQLAQLISSVQKNRSVHTPSGDLYFKNSGTPIQCLLIPGNEKVKAVANHLAQLNMEVR
                     PILYPTVPEGEERLRIALHSYNTPEEVQELISGLNIRVRS"
     gene            complement(1893..>2414)
                     /locus_tag="VGM24_09740"
     CDS             complement(1893..>2414)
                     /locus_tag="VGM24_09740"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEY4155702.1"
                     /translation="DKWQFSVWDNLNLKLRGEFKYRFSIGGFFNTRKLFIQDYQHFNG
                     NQTFYASPYLNSFQIAPYYANSTTAAFYAVGHVEHHFNGMITNKIPLLRNLQWNLVAG
                     SNAFYVNRNNNYVELFGGLENILKIFRVDVVASYLNGKTGQVGVRIGLDGLFGGFISA
                     ALKNPGGRPPQRQ"
BASE COUNT          578 a          600 c          612 g          624 t
ORIGIN      
        1 aaaggataag cggtcatctt cttaaaggtc cggggaaaat caaccattaa tgaatgcggt
       61 ctccccgaac gtctattttt gatatgatgc cggcctcata ctgcagccat tctgcccagc
      121 gcttacgcac tttttctttt ccgaggtagg tttcggcaag atcgatgaaa agatggtaat
      181 ggcccgcctc gctttccatg aaacgccggt aaaagtttct caggtattca tcttttagac
      241 cttcgcttag ccgtttgaaa cgctcgcagc tgcgggcttc gatcatggcc atggtcagca
      301 gctggtccag gaaacggtct tcaaaatccc cgcccttgcg ctggaatgag atcaggtgat
      361 tcacatattc atctttccgc tgtttaccca attttaaccc gcgtttggtg agttcggcga
      421 gcacctggcg aaaatgcccc cattcctccg taacaacggg cgcaaggtcc gataccagcc
      481 gtgtgcggtc ggaatatttc tgaataagcg aaatgcaggt gatcgcggct ttttgttcgc
      541 agaaagcatg atccgtcagg atctcctcaa gggaaaggct tgccaggtcc acccatcggg
      601 gatctgtgcc aagctgaagg ccgaggatgt ttttggcgtt actcatggtt tggcggttgt
      661 cctgattaaa aggatctttg gccgctaaag ataggaatta ggcatattcc gggaatggtt
      721 tttgacctac ccggcattag aaacttcaaa aaatgaattc cgattttctg gatcgtaaac
      781 tgcaggaaag atttagccgg aatgcttacc ggcggttgca gctggccgaa ggcaagcatg
      841 attttttttc caatgattac ctgggcattt ccaaaaacgg attgatcgaa gcagcgctgg
      901 agggaacgtc cttttctcct ggttcaacgg gttcccgttt gcttgcgggg aattatgccc
      961 tgatggagga aacggagaac cggattgcgg aattccacca atcgccttcc gccctgatct
     1021 ttaattccgg atatgacgcc aatctgggtt tgctggcttc tgtggcggat agaggggata
     1081 ccattttata tgataaactg agccacgcct ccatccggga tggcatccgg ctttcccttg
     1141 cagaggcttt ttctttccag cataacagtc cgggagatct cgaaaagaaa ctgcaaaaga
     1201 aaaaaggaaa ctgctttgta gttaccgaat ccgttttttc catggatggg gatcaggccc
     1261 cgctggccga actggttccc ctttgcgaac ggtttggcgc ccacctcatc gttgatgaag
     1321 cccatgcaac aggggttatc ggcggccggg gggaaggtct ggtgcagctg gaaggacttc
     1381 agcaacgctg cttcgccagg gttcatactt ttggcaaggc gctgggttgt cacggcgcag
     1441 ccgtcctggg cagtgagatg ctgaggaact acctgatcaa tttttcaagg cctttcatat
     1501 ataccaccgc gcttccgccg tccgccgttg cggcgatccg ggcctcgtat gaaatttttc
     1561 ccggcttaaa tccggaaagg cggcaactgg cccagctcat ttcgtctgtt caaaaaaacc
     1621 ggtccgttca cactccctcc ggagacctgt attttaaaaa ttccggcacc cccatacaat
     1681 gcctgctgat tccggggaac gaaaaggtaa aagcggttgc caatcacctg gctcaattga
     1741 atatggaggt acgcccgatc ctttatccaa ccgttccgga aggagaagaa aggctgcgga
     1801 ttgcattgca tagctataat accccggagg aggtgcagga attgatttcc gggctgaata
     1861 taagagtccg ttcttaaaaa aacttcgaat ggtcactgtc tttgcggcgg tcttcccccc
     1921 gggtttttga gcgctgcact gatgaacccc ccgaatagcc catccaggcc gatccggaca
     1981 cccacctgtc ctgtttttcc gttgaggtag gaggccacca catccacccg gaagatcttg
     2041 aggatatttt ccagcccccc gaaaagttcc acgtagttat tattccggtt tacataaaaa
     2101 gcatttgaac cggccaccag gttccattgc aggtttctta aaagcggaat tttgttggtg
     2161 atcatgccat tgaaatgatg ttccacatga ccgaccgcat aaaaggcagc ggtggtgctg
     2221 ttcgcataat agggagcaat ctgaaaactg tttaaataag ggcttgcata aaaagtctgg
     2281 ttaccgttga agtgctggta atcctggata aataatttcc gggtattaaa aaagccgccg
     2341 atgctgaacc ggtatttgaa ttcaccccgg agtttcaggt tcaggttgtc ccacaccgaa
     2401 aattgccatt tatc
//
LOCUS       DASYCU010000483         3895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_978648, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000483 DASYCU010000000
VERSION     DASYCU010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978648"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1506)
                     /gene="xseA"
                     /locus_tag="VGM24_09745"
     CDS             complement(67..1506)
                     /gene="xseA"
                     /locus_tag="VGM24_09745"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY4155703.1"
                     /translation="MTAIHHLRLSELAGRISEALQARFASASFWVIADITNHTLRESK
                     NYHYFELVEKDPDSNNLLAKVSGKAWGNGAVRILNFETVTGQPFTNNIRVLVNVSVEY
                     HPVRGLQLNLIDIDPHFTLGVLEQQRRATLEKLVTENPDFIKKAGDRYLSRNNQLKLN
                     RVIQQIALISSRTSAGGEDFKHTLLNNRFGYHFHIDEYYTPVQGDHNADLFIAKIIEV
                     FNARKPYDALVITRGGGSQTDFLIFDNYRIGKAVAKFPIPIITGIGHQKNETIADLMA
                     NTQTKTPTKAAEFILDHNRKYEEEMLSFQKNIVIRTQQLFSSHFQNLASLKSQVVHKA
                     GNKLTLNKDRLVRMNQVTINVSKSILFKSASSLVNLSSRIVSKPGSILYSRKKDILHL
                     AGNIKTFNAQYLKNQTAYLGHFRSIIKMMSPENILKKGFALVKQNDKIISHPDDIIIG
                     KEVDIILSDTLIRSAVKQKIKYDGKDFNI"
     gene            complement(1544..2314)
                     /locus_tag="VGM24_09750"
     CDS             complement(1544..2314)
                     /locus_tag="VGM24_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY4155704.1"
                     /translation="MVTGRHHYILERINREGSVSVLELCKELKVSSVTIRKDLKLLEN
                     KNLLFRTHGGANATNPYTIDRPVNEKEKLQSGEKNRIGAAAAKLVQPNDSLIIASGTS
                     VLAMAKKIQSPESTTIITSALNVALELIKHPNIEVILLGGMLRKSSSSVTGPYAQNIL
                     NDFFCTKLFLGVDGIDLDLGLTTTSSMEAQLNRQMIAASQKIIVLTDSTKFGKRGFGR
                     ICNLEEIDQIITDSGIPEHYKKTLESMGIEITIIEEAK"
     gene            2476..>3895
                     /locus_tag="VGM24_09755"
     CDS             2476..>3895
                     /locus_tag="VGM24_09755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY4155705.1"
                     /translation="MNQTNCFDRACQLKLVKETVKWDLIVIGGGATGLGVAVDAASRG
                     YRTLLLEQSDFAKGTSSRSTKLVHGGVRYLAQGQIKLVYNALHERGILLHNAPHLVKK
                     QAFIIPCYGIWEEIKYLTGLKIYEKLSGRFSFGQADFIKRKEINKYLPVLRTKGLRGG
                     VRYYDGQFDDARLALNLAQTASEKGAAILNYFKVVGLLKEGGKTAGVSVVDMETKEEY
                     TLKTRVVVNATGVFVDDILKMENAAAKPMIRPSQGVHLVLDKSFLNSSTALMIPKTSD
                     DRVLFAVPWQDHVLVGTTDTPTDKHLLEPKALDREIGFILDTVRQFLAAPPERKDVLS
                     VFAGLRPLAAPENDEHPTREISRDHKLFVNPSGLITITGGKWTTYRKMAEETVNKAIE
                     VGGLMPQPCQTKDLRIHAYRGSGSTGHLSVYGSDEEEIKALVEEEPVLGNRLLHRFPY
                     TEAEVIWAVRNEMARTIEDVLAR"
BASE COUNT          934 a          901 c         1017 g         1043 t
ORIGIN      
        1 atatctacag aaacggattc cgtttcaatc tctcccgcta tccgggccaa ttcgtcgtag
       61 gcatcttcat atgttaaagt cttttccatc gtatttaatt ttttgtttca cggccgaccg
      121 gatcagggta tccgaaagaa ttatatccac ttccttacca atgattatat cgtccggatg
      181 gctgattatc ttatcattct gcttcaccag ggcaaagccc ttcttcaaaa tattttccgg
      241 cgacatcatt ttaattatag aacggaaatg acccagatag gcggtttgat ttttcaggta
      301 ctgggcattg aaggtcttaa tattgccggc cagatggagg atgtcctttt ttctgctgta
      361 aaggatactt cccggttttg atacgatgcg ggaagagagg ttaaccaggg aggatgccga
      421 tttgaaaaga attgatttgg aaacattgat cgtcacctgg ttcatccgca cgagccggtc
      481 cttattcagc gtcagcttat ttccggcctt atggacgacc tggcttttca gcgaggcgag
      541 gttttgaaaa tgcgaggaaa aaagctgctg ggtacggatt acgatattct tctggaacga
      601 aagcatctct tcctcatatt ttctattgtg gtccaggatg aattccgccg ccttcgtggg
      661 ggtctttgtt tgtgtattgg ccatgaggtc ggcgatggtt tcgtttttct gatggccgat
      721 cccggttatg atggggatgg ggaattttgc aactgccttt ccgattctat agttgtcgaa
      781 gatcaggaaa tcggtttgcg agccgccgcc ccgggtaatc accagggcat cgtagggttt
      841 ccgggcatta aaaacttcga ttattttggc gatgaacagg tcggcattat ggtctccctg
      901 aaccggcgta taatactcgt caatatggaa atgatagcca aagcggttat tcagcagggt
      961 atgtttaaag tcttcgccac cggcggatgt cctggaagaa attagggcga tctgctgtat
     1021 cacgcggttc agtttcagct ggttgtttct gctgaggtac cggtcgccgg cttttttaat
     1081 gaaatcggga ttttccgtta ccagtttttc aagcgttgcc cgtcgctgtt gttccagaac
     1141 tcccagcgta aaatgcgggt caatatctat gagatttaac tgtaacccgc gaaccgggtg
     1201 gtactcaacg gaaacattga ccagtacccg gatattattg gtgaacggct ggccggtcac
     1261 ggtttcaaaa ttcaatattc tgacggcgcc atttccccag gccttcccgg aaaccttggc
     1321 gagcaggtta tttgagtccg gatctttttc caccagttca aaatagtgat agttctttga
     1381 ttcccgcagc gtatgattgg tgatatcggc aattacccag aagctggctg atgcaaagcg
     1441 ggcctgaagc gcttcgctga tcctgcccgc aagttcggaa agccgtaggt gatgaattgc
     1501 tgtcatgccg ctatgttcaa agggaatttt tttttcgggg aaactatttt gcctcctcaa
     1561 tgattgtgat ttcaataccc atgctttcca gggtcttttt atagtgttcc ggaatgccgc
     1621 tgtctgtaat gatctgatct atttcttcga ggttgcaaat tcttccaaaa ccccgtttcc
     1681 cgaattttgt tgagtccgtc agcacgataa ttttttgcga cgccgcaatc atttgccggt
     1741 ttagctgggc ttccatggaa ctggtggtgg tgaggcccag gtcaagatct atcccgtcca
     1801 cgcccagaaa cagtttagtg caaaaaaaat cgttcagaat attctgtgca tagggtccgg
     1861 taactgagga tgaacttttg cgcagcatgc cacccagcag gatcacttca atattcgggt
     1921 gtttgatcag ttccagggca acattcaatg ctgaagtgat gatggtggtg gattcggggg
     1981 actggatctt tttggccatg gccagtacgc tggtgccgga agcaatgatg agggaatcat
     2041 tgggctgaac aagttttgca gcggcagcgc cgatcctgtt tttttcgccg gattgcaatt
     2101 tttctttttc attgacgggc cggtcgatgg tgtacggatt ggttgcattg gcgccgccat
     2161 gggtccggaa aagtaaattc ttgttctcca gcaattttaa atccttccgg atcgtaaccg
     2221 atgaaacctt cagctcctta cacagttcca aaacgctcac gctcccctct ctgttgatcc
     2281 tttccaggat ataatgatgt ctgccggtaa ccatataagc ggtagttctt aaagtaaata
     2341 tagggaaaca aggattaacc cggttccgga aggctgtttc gttttgtttc tatttatatt
     2401 tttttatttc gattcatttt atttagtttt gaattattga tcctgatgac ctgaaaagga
     2461 aatactctga aatagatgaa ccaaacgaat tgttttgacc gtgcctgcca gctgaaactg
     2521 gttaaggaaa ccgtgaaatg ggacctgatc gtgatcggtg gtggggcaac cggcctcggg
     2581 gtcgccgtgg atgccgccag ccgggggtat aggaccctgc tcctggaaca gtctgatttt
     2641 gccaaaggca cctccagccg gagcaccaaa ctggttcacg gaggtgtgcg ctacctggcc
     2701 caggggcaga tcaaactggt ttataacgcc ctgcacgaaa ggggaatcct gcttcacaat
     2761 gccccgcacc tggtcaaaaa acaggctttt atcatcccct gttacggtat ttgggaggaa
     2821 ataaaatatc tgacgggtct gaagatctac gaaaaacttt ccggccgttt cagtttcgga
     2881 caggcggatt tcataaaacg aaaggagata aacaaatacc tgccggtcct ccggacaaaa
     2941 ggactgcggg gcggcgtccg ctattatgac ggacagtttg acgatgcccg cctggccctg
     3001 aatctggccc agacggcctc ggaaaaaggc gcagccatac ttaattattt taaagtggtg
     3061 ggcttgctta aggagggcgg aaagacggcc ggcgttagcg tcgtggacat ggaaacaaaa
     3121 gaagaataca cgctgaagac gcgggttgtc gtaaatgcga ccggcgtatt tgtggatgat
     3181 atcctgaaaa tggagaatgc cgcggcgaaa ccgatgatca ggcccagcca gggcgtgcac
     3241 ctggtgctcg acaaatcttt tttgaacagc agcaccgcgc tcatgatccc caaaacctcc
     3301 gatgaccggg tgttgtttgc cgttccctgg caggatcacg tgctggtggg aacaacggac
     3361 acgccgaccg acaaacatct gctcgaaccg aaggcgctgg accgggagat cgggtttatc
     3421 ctggatacgg tcagacaatt cctggcggct ccgccggaac gcaaagatgt tttaagcgta
     3481 tttgccggct tgcgtccttt ggccgcgccg gaaaacgatg aacacccgac cagggaaatt
     3541 tcgcgcgatc ataaattatt cgtaaacccc tcgggtctga tcaccatcac cggcggcaaa
     3601 tggacaacct accggaagat ggcggaagaa accgtgaata aggcgattga agtgggcgga
     3661 ctgatgccgc agccctgcca gacaaaagac ctgcggattc atgcttaccg tggttccgga
     3721 tcaacgggtc atttatccgt ttacgggtcc gatgaggaag aaataaaagc attggtcgaa
     3781 gaagaacccg tgctcggaaa ccggctgctg catcgattcc cttacaccga agccgaagta
     3841 atctgggctg tccggaatga aatggcgcga acgattgaag acgtgctggc ccggc
//
LOCUS       DASYCU010000484         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1264060, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000484 DASYCU010000000
VERSION     DASYCU010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264060"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1653
                     /locus_tag="VGM24_09760"
     CDS             124..1653
                     /locus_tag="VGM24_09760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY4155706.1"
                     /translation="MKATFLISLILVFGQFVHAQSPNWQNLDLQKDSVFGISTEKAYA
                     ELLQHKQKKKKYKPVLVAVIDGGVDTSHEDLKSLIWINPKEIAGNGLDDDHDGYSDDV
                     HGWDFIGGSKGDIDQENLELTRLIRKGEFTADMLEEYQKQVRKDSSNVRNIKSFERVL
                     DGMTAKMKKDSPSLSDFQSYTPEGPGEKQIKQVMIQVLQQDSDFTAFRENEIEDAIKH
                     FQTELDYHLNISFNPRAIVGDDSTNAEEHLYGNNDVTGPDALHGTHVAGIIGAERNNN
                     IGIRGVADRVRIMSVRTVPDGDERDKDVASAIRFATDHGARVINMSFGKGYSPNKKVV
                     DEAVKYAMSKDVLIVHAAGNEGENLDHESNFPNRTYADSSGAADAWIEVGASGWKNDF
                     TLVAPFSNYGRNTVDVFAPGVKIRSTIPGSKYIDESGTSMASPVVAGLAALIREYYPK
                     LKASEVKDIIMKSVVKPTQNVTVKDGRKALSLHFSEVCVSGGVVNAYNALSLAKHYKG
                     K"
BASE COUNT          494 a          380 c          437 g          389 t
ORIGIN      
        1 caggggaagg tattccttaa taacccgctg aggccccgcc catcgttatt ttcaccgttc
       61 atcaaggatt agtacaattt tattttatat tccgcgtatt tttatcttta agcgatatat
      121 agaatgaaag ccactttctt aatttccctg atattggtat tcggtcagtt tgtccatgcc
      181 caatctccca actggcaaaa cctcgatctg caaaaagaca gcgtatttgg catcagcacc
      241 gaaaaagcat acgctgaatt gcttcagcat aaacaaaaaa agaaaaaata taaacccgta
      301 ctggttgctg tgatcgacgg cggcgtggat accagccacg aagacctgaa atccctgatc
      361 tggattaatc caaaagagat cgcgggcaat ggcctggatg atgaccatga tggctacagc
      421 gatgatgtgc acggctggga ttttatcggc ggttcgaagg gcgatatcga tcaggagaac
      481 ctggagctca cacgtttgat ccgaaaagga gaatttacag ccgacatgct ggaagaatat
      541 caaaagcagg tcaggaagga cagcagcaat gtcagaaaca ttaaaagttt tgagcgggtg
      601 ctggacggca tgaccgctaa aatgaaaaaa gacagtcctt cgctttcgga ttttcaaagc
      661 tatactccgg aagggccggg ggaaaagcaa attaaacaag tgatgatcca ggtcctgcag
      721 caggattccg actttactgc gtttagagaa aatgaaattg aagacgccat aaaacatttc
      781 cagactgagc tggattatca cctgaatatt agttttaacc cgagggcaat cgtaggagat
      841 gacagcacga acgcggaaga acatctctat ggcaataatg atgtaacggg cccggatgcg
      901 ctgcacggaa cgcatgtcgc cggcatcatc ggtgcggaac gaaataacaa tatcggtatc
      961 cggggtgtcg ctgatcgggt ccgcatcatg agcgtccgca cggtacccga tggcgatgaa
     1021 cgcgataaag atgtggcgag cgccatccgt tttgcaaccg accacggtgc cagggtgatc
     1081 aacatgagct ttggcaaggg ctattcgccc aataagaagg tcgtagacga agcggtcaaa
     1141 tatgcgatgt caaaagatgt attgatcgta catgctgcgg gcaatgaggg cgagaatctg
     1201 gaccacgaaa gcaatttccc gaatcgcact tatgcggaca gcagcggagc ggctgatgcc
     1261 tggatcgaag tgggcgcttc aggctggaaa aatgatttca cgctcgtggc tccattttcc
     1321 aattatggaa ggaatacggt agatgttttt gctccgggcg taaaaatccg ttctacgatc
     1381 cccggttcaa aatatataga tgaaagcggc accagtatgg cttctccggt agtggccggc
     1441 cttgccgccc tgatccggga atattatccc aaactgaagg cctccgaggt aaaagacatt
     1501 atcatgaagt ctgtggtgaa gcccacccaa aatgtgaccg ttaaagacgg gcgaaaagcc
     1561 ctttcccttc atttttcaga agtctgtgtt tccggcgggg tggtgaatgc ctacaatgcg
     1621 ctaagccttg caaaacatta taaaggaaaa taaagcccgg gctccctcca ggagcagatt
     1681 ttaaaagagg tccgggatat
//
LOCUS       DASYCU010000485         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4456140, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000485 DASYCU010000000
VERSION     DASYCU010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4456140"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VGM24_09765"
     CDS             <1..1392
                     /locus_tag="VGM24_09765"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4155707.1"
                     /translation="ITVFDLPYAIENLAYQTAVGKVAPVLRTEGGYLILKKTAERPAW
                     GRMRAAQILLIFPYHANDSAKADTRRRADSLYRAIRAGSDFGELARKYSGDNLSYQLG
                     GILPEFGIGKYEAGFEHVAYSLKKDGDISEPFATEFGYHILKRIARIPAPDKRDKKTL
                     DGLKERVKADRRIEISRNEMLQTILKKTGFKQITPAGSPLFAYTDSMLSNKKEPPNPG
                     INAGTILYRFPDKTVTVGDWVEYRKSLNRIPSLINGKTPYDLLDQYRQTVAFDYYKAH
                     LEKFNRDFGYQVKEFRDGNLLFEIMQREVWDKASADTAGLRSFFESHARDYWWKASAD
                     AIIFNAGNEAAAERIRNDFRGNIPNWRRWVDSLGGQVQADSGRFDLKQLPGKGDADEQ
                     SFTSMTTNADKTVQFAYIIRKHPDPAARTFDDARGLVINDYQNGLENKWVAELKKKYP
                     VRVDEAVFKTLLK"
     gene            complement(1393..2241)
                     /locus_tag="VGM24_09770"
     CDS             complement(1393..2241)
                     /locus_tag="VGM24_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HEY4155708.1"
                     /translation="MRIACVIIAHKGPQQIERFIKKFEHFPFDFYVHLDKKTAREPFD
                     RLADIPNVQFIQPRIKVRWGASSVVTAMLLSIKTVLKSGLEYDFINIMTGQDYPIKPV
                     STIYSTLEKNRGKNFICYEDDGEWWSHAIARIQKYHFTDFGFRGRYRLQFLMNTLLPE
                     RKFPLPYTLYGGPRGMCMILSTDCLKYVVNLIDSNKRLRRFIRLTWGADEFLIPTLIM
                     NSEFRKTVVNNNFYYIDWTGGGTNPKTLTTEDYESLCQSDKMFARKFDMARDPAILDM
                     LDKGER"
     gene            complement(2289..>3008)
                     /locus_tag="VGM24_09775"
     CDS             complement(2289..>3008)
                     /locus_tag="VGM24_09775"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY4155709.1"
                     /translation="DYQGWDLVKLPGVYKAGIPTLSGTGAEVSRTTVLTGPTRKLGMN
                     SDFTPFDQILLDPELTANAPVNQRFYTGMDCYIHCIESLTGTFLNEFSKSYGEKALNL
                     CRKVFLDDGGWTPLHDDRLMMASYAGGMSIAYSQVGVAHAVSYGLGYLLGTRHGVGNC
                     IVFDKLEDYYPEGVKEFKKMVEKNRIEIPQHITKGLTNDKFETMINVAMGMKPLWENA
                     LGPDWEKIMTRDKLRMLYEQL"
BASE COUNT          790 a          725 c          748 g          745 t
ORIGIN      
        1 atcacggtct ttgaccttcc ctacgcaatt gaaaatctgg cctatcagac agcggtagga
       61 aaagttgctc cggttttgag aacggaaggc ggttatctca tcctgaaaaa aaccgcggaa
      121 agacccgcct ggggccgaat gcgggcggcc cagatcctgc tcatatttcc ttatcacgcc
      181 aatgattcgg ccaaagcaga tacccggcga agggcagact ccctgtatcg ggccatacgg
      241 gccggatccg atttcgggga actggccaga aaatacagcg gggataattt atcttaccag
      301 ttagggggaa tactgcccga gttcggaatc ggaaaatatg aggccggttt tgaacacgtc
      361 gcctacagcc ttaaaaaaga cggggatatc agcgaacctt ttgcaacaga attcggatac
      421 catattctga aacggatcgc acggattccg gctccggaca aacgggataa gaaaacgctg
      481 gatggcctga aggaaagagt gaaggcggac cgccgcatcg agatttcccg gaatgaaatg
      541 ctgcaaacta ttttaaagaa aacgggattt aaacaaatca ccccggcagg gtcccccttg
      601 tttgcttata cggacagtat gctgtccaat aaaaaagaac cgccaaaccc cggtataaac
      661 gcgggcacga tcctttaccg gttccctgat aaaaccgtta cggtggggga ttgggttgaa
      721 taccgcaaat ccctgaaccg cataccttcc ctgatcaatg gaaagacccc ttacgacctg
      781 ctggatcaat accggcagac cgttgcattt gattattaca aagcacacct cgaaaaattc
      841 aacagggact tcggttacca ggtaaaggaa ttccgggatg gtaatttatt atttgaaatc
      901 atgcagcggg aggtgtggga taaagcctcg gcagatacgg ccggactaag aagctttttt
      961 gaatcgcatg cccgcgatta ctggtggaag gccagtgcgg atgcaattat tttcaatgcc
     1021 ggtaacgagg ctgcggcaga aaggatccgg aatgatttcc gggggaatat tccgaactgg
     1081 cgcaggtggg tggatagcct gggcggacag gttcaggccg attcgggaag attcgatctg
     1141 aaacaattgc cgggaaaagg ggacgcggat gaacaatctt tcacgtctat gacgaccaat
     1201 gccgacaaga cggtccagtt tgcttacatc atccggaagc atcccgatcc ggcggcccgg
     1261 accttcgatg atgcccgcgg cctggtgatc aacgattacc agaacggact tgaaaataaa
     1321 tgggtggctg aattaaaaaa gaagtatccg gtgcgggtgg atgaggccgt gtttaagacc
     1381 ctgctgaagt aatcatcttt cccccttatc cagcatatcg aggatagccg ggtcccgcgc
     1441 catgtcaaat ttccgggcga acattttatc tgattggcag agcgattcat agtcctctgt
     1501 ggtaagtgtt ttcggattgg ttccgccccc ggtccagtca atataataga aattattatt
     1561 gacaaccgtt ttgcggaatt ccgaattcat aatgagggtc gggatcagga attcatctgc
     1621 accccaggta agccggataa agcgccgaag gcgcttattc gaatcaatca ggttcaccac
     1681 atacttcagg cagtcggtac tgaggatcat gcacattccg cggggtccgc cgtataaggt
     1741 atagggaagc ggaaatttcc tttccggcag cagcgtgttc atcaaaaact gaagccggta
     1801 ccttccgcgg aacccgaagt cggtaaaatg gtatttctgg atacgggcaa tcgcatgact
     1861 ccaccactcg ccatcgtctt cataacaaat aaaattttta cccctgtttt tttccagtgt
     1921 ggaatatatc gtgctgaccg gtttaatggg atagtcctgc ccggtcatta tattgataaa
     1981 atcatattcc aggccgcttt tcaatacggt cttaatggaa agtaacatgg ccgtgaccac
     2041 gctcgaagcc ccccatctta ccttgatccg gggctgaata aactgtacgt tgggaatgtc
     2101 ggcgagtcga tcaaagggtt cccgggccgt tttcttgtcc aggtgaacat aaaaatcaaa
     2161 agggaaatgt tcgaattttt ttatgaagcg ctcaatttgc tgaggtcctt tatgagctat
     2221 tatgacgcag gcaatccgca tagtgattct aaatataggt taacagaggt ggacagaaat
     2281 cagccggctt ataattgttc gtacagcatt ctcagtttat ctcttgtcat gatcttttcc
     2341 caatccgggc ccagggcatt ttcccacagt ggtttcatgc ccatggccac gttgatcatg
     2401 gtttcgaact tatcgttcgt caatcctttt gtaatgtgtt gaggaatttc gatccggttt
     2461 ttttcgacca tctttttaaa ttcctttacg ccttccggat aatagtcctc caatttatca
     2521 aaaacgatgc aattcccaac accgtgcctg gttcccagta aatagcccag gccgtaactc
     2581 acggcatgag caacgccaac ctgcgaataa gcaatactca tacctcctgc ataggaagcc
     2641 atcatcaggc ggtcgtcatg tagaggggtc catcccccgt catccaggaa caccttccgg
     2701 catagattga gcgctttttc tccatacgat ttgctgaatt cattcagaaa ggttcccgta
     2761 aggctttcaa tacagtgtat atagcaatcc atgccggtat aaaaccgttg atttaccggt
     2821 gcattggcgg taagttcagg gtcaagcagg atttgatcaa aaggcgtaaa gtcggagttc
     2881 atgccgagct ttctcgtggg gccggtcagc acggttgtcc gggaaacttc cgcacccgtg
     2941 ccgctcaggg tgggaattcc tgctttatat accccgggta attttaccag gtcccagccc
     3001 tggtaatc
//
LOCUS       DASYCU010000486         7137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1847040, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000486 DASYCU010000000
VERSION     DASYCU010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847040"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1158)
                     /locus_tag="VGM24_09780"
     CDS             complement(214..1158)
                     /locus_tag="VGM24_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155710.1"
                     /translation="MKKLILFLFSALVWYSPKAQRCLDIRTGAAPALIRLSPDAASAF
                     TDCVTGRNDHGALQIANYGKNVSDLDTLLSRNTLAFTMASVGNLNAGLPPASDVDAAK
                     QLAQKMNSMTDEQKKAFAMQIAQQQIQNAGHSAVVQDDPATIKLVYQTQDIASRQLRA
                     LDDEFSAKLKAVQASAEKEEQAMKEPDLHKCPSVVKEGLPSCACVNALYVPYWKQKVQ
                     IQDKYNKQRTEILNSYLPRIRALCAQVDENVRSLNYGDKIKAAQLKQLLLSSQSAAFA
                     NAALVTVGCTEDIRKTGSDLYVNQVNSEHNVYDISCEK"
     gene            complement(1186..2448)
                     /locus_tag="VGM24_09785"
     CDS             complement(1186..2448)
                     /locus_tag="VGM24_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY4155711.1"
                     /translation="MKRILLLAGGLYICLSVCTVRAQVNDPNQVAQDAAATHANNDMD
                     IAADKGLNKAGQGIGNLFKKKKEKGSAKSGTPASGPAVPEGNQKNPNASLSQEPPIKT
                     YQNYDFVAGDKIIFEDDFISDQDGEFPAHWELKKGQGAVNKQQGFQTFTLTDGNYVVV
                     NPRVSTESYLGNEFTVEWDVYSEAGSYGTHLFFHLPAVTEDQDADLSINKDEVSYNSQ
                     DANIDMNGSLPSDIAGDNHINKWHHIAVGYKNKQIKVYVDQYRVLVVPDCKMVFGSLS
                     FGGSASQDAPILFKDVKMASGGSQNMIGNILTNGKFVTHGITFDIDKAVLRPESMGVL
                     NELAKFLKENGSVNMEIDGHTDNSGAAAHNLTLSQQRADAVKAQLLSMGIDGARLSTK
                     GFGDTRPNADNGSPEGRANNRRVEFVKK"
     gene            complement(2534..3598)
                     /locus_tag="VGM24_09790"
     CDS             complement(2534..3598)
                     /locus_tag="VGM24_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY4155712.1"
                     /translation="MNKRWVRIGFILAFLYRPDISIGRQPAPYPVYLSNYTFSAGRGQ
                     AVLFRVLGDSVQQATLRLAGSNAGLFKLEGNNILLSGQGKKYFKKFSRASFSITFSSG
                     NKTGMEQTFTILKDNFAANKVIAHRGAWKNTGLPQNSIASLQAAIRMGCAGSEFDIQS
                     TADSILVINHDPTYEGMGIEHTVYTDLLKYPLQNGEALPTFEHYLKTGMEQEKTRLIA
                     EIKPSVIDQTHVLELAKRVVEMVHREQAQAWVIYISFDYDALKEVLRLDPAAVTLYLN
                     GDKAPDILKKDGIRGMDYQYRVYHKEEQWIKGALDAGLDLNVWTVDTVPEMEYFLARQ
                     VGYITTNEPENLFALLPGSK"
     gene            3835..6618
                     /locus_tag="VGM24_09795"
     CDS             3835..6618
                     /locus_tag="VGM24_09795"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY4155713.1"
                     /translation="MRITLIITALFFLCQPLFSQQASIKGLVSDTSEQASLRQSSIMI
                     LQRKDSFLIAFTRADARGAFSFLHLPAGPVICKISYPGYADYVTELDLKDSTETNLGT
                     VPMTLKSKLLQEVIVHGNMAAIRMKGDTLEFKADSFKVQQGATVEELLKQLPGIQVDR
                     NGKITAQGETVKKVLVDGEEFFGDDPTLVTQNLRADMVDKVQVYDKKSDQAAFTGIDD
                     GERNKTINLQLKEDKKKGYFGKLEAGAGTNGFYNNQAMFNLFKKKLKFSGYGILSNIG
                     TIGLNWQDNDSYGQSFASSVDYDETSGYFSFNGNGDDLESWNGQYNAQGLPTVKTAGL
                     HFNNKWNQDKQSVNLNYKILDLDVTGKNESRSQYLLPDTLYYNNQTESFHNHILRNKA
                     SGIYEFNLDSSSSLKINVDANKDHKTTQSNFTSEYRAEDSSLVNRNNRNVGTTGDEEK
                     LNTNLLWRKKFHRKGRTLSFNFRENYKQNNSTGYLYSDTRFFGRDSLNADQLTDQYKT
                     FLNKTLTLDSRLAYTEPLSEISSIAVNYGISGGNSQSYRNSFNKSTGGKYDALDSGYS
                     NSYHFSLLSQKTGLLYNFTGRKLRFNVGADVGFTGFDQKNMDNGTTLHRHFVNWYPQS
                     RISYKFAQEKSLFFRYSGNTQQPGIEQLQPLANNQDPLNIYVGNPDLKPAFVNSFGLN
                     YFNYKPLSETGLFINMYGNATSNAISSKDYVDSLGRRVYQSVNVNGNYSLNGNINYDI
                     KLKKSEINIGLGTGFGANRYKNIVNDESNETNQKNYSFSLRAGKSKQKKYDISLSGSA
                     NYTTSISSIQQAINTHFWSFEFSPDLGLYLPLKFELHTNCEFNIRQKTTAFDTNTNTA
                     LWNGWIGKKFFKQESLLLKLQANDILDQNIGFNRTVNSNYISQSTYTTIRRYFMFSVV
                     WNFTKAGATPSKP"
     gene            6620..>7137
                     /locus_tag="VGM24_09800"
     CDS             6620..>7137
                     /locus_tag="VGM24_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HEY4155714.1"
                     /translation="MRNVFCFIPLLLFCYLESNAQQAIFLSEGKVEFTRTVNLYARMN
                     NDDESFSELLKKSMPKFKKTYFQLFFSNNQWIYKPGRENPENNRLWEEPAENNIVSVD
                     YTTGKSVSQKEIFGEMFLVQDSLRRIKWKMTDETRVIAGFNCHRANALIMDSIYVVAF
                     YTDEIITPGGPES"
BASE COUNT         1889 a         1787 c         1641 g         1820 t
ORIGIN      
        1 tttccattta tttttgtgct taaggtgagt ttaatccgga gaagatcaat ggctttagga
       61 aggttgcccg ctaaagcaga aaccaatata caaatatttt catttacaac ccgcggtctt
      121 cttttatcct ccccacggct gtcctttgga agcggctatc ccggccagcc ttgccaggcc
      181 ccgttattcg ccggccttac tctttactac cggctacttt tcacaggaga tatcgtacac
      241 attgtgctcg ctgttcacct gattcacata caggtccgaa ccggtcttcc ttatatcttc
      301 cgtacagccg accgttacca gagccgcatt ggcaaaggcg gcggactggc tgcttaagag
      361 cagctgttta agctgcgctg cttttatttt gtcaccataa ttcagactcc ggacattttc
      421 atccacctgg gcgcagagcg cccggatcct gggcagatag ctgttgagaa tttccgttct
      481 ctgcttattg tatttatcct gtatctgaac cttttgtttc cagtaaggaa catacagggc
      541 atttacgcag gcgcaggaag gcaggccctc tttcacaacc gaaggacatt tatgaaggtc
      601 cggttctttc attgcctgtt cctccttttc agcactggcc tgcacggctt tcagtttcgc
      661 cgaaaattca tcgtcgagcg ctctcaactg acggctggcg atgtcctgag tttgataaac
      721 cagtttaatg gtcgccggat catcctgtac aactgcacta tgccctgcat tctgtatctg
      781 ttgctgggct atttgcatgg caaacgcctt tttctgctcg tcggtcatgc tgttcatctt
      841 ttgcgccagt tgctttgccg catcaacatc cgatgccggg ggcaggccgg cgttgaggtt
      901 tccgacgctg gccatagtga acgccagggt attccgggaa agcagggtgt ccaggtcact
      961 gacattcttt ccgtagttgg cgatctgcaa ggccccgtga tcattcctgc ccgttacaca
     1021 atctgtaaag gcgctggcgg catccggaga aaggcggatc agcgccggcg ccgcaccggt
     1081 ccggatatca aggcagcgct gtgctttcgg tgagtaccat accagcgcgg agaaaaggaa
     1141 aaggatgagt tttttcatgg ccaaaaattt agggttatat ggttgtcatt tttttacaaa
     1201 ctccacccgc cggttatttg ccctcccttc cggactgcca ttatcggcat ttggccgggt
     1261 atctccgaat cctttggtgg aaaggcgggc cccatcgata cccatgctca gcaactgggc
     1321 cttcacggca tcggcacgct gttgggagag cgtgaggtta tgggctgcgg cgccactgtt
     1381 atccgtatgc ccgtctattt ccatattcac ggaaccattt tccttcagga attttgccag
     1441 ctcgttcagc acgcccatgc tctcgggccg gaggacggct ttatcaatat caaacgttat
     1501 accgtgtgta acaaattttc cattggtcag gatattgccg atcatattct gactgccgcc
     1561 ggaagccatt tttacatcct taaataggat gggggcatcc tggctggccg agcctccgaa
     1621 agagagcgac ccaaagacca tcttgcaatc gggaacaacc agcacccggt actgatccac
     1681 atatacttta atctgcttat tcttatagcc aacggcaatg tgatgccatt tattaatgtg
     1741 gttatccccc gcgatatcgg aaggcaggct gccgttcata tcaatattgg cgtcttggct
     1801 gttataagaa acctcatcct tgtttatact aagatccgca tcctggtcct cggtaaccgc
     1861 gggcaaatgg aaaaaaagat gcgttccata agacccggcc tcggaataga catcccattc
     1921 gaccgtaaac tcatttccaa gatagctttc tgttgaaacg cgggggttca ccaccacata
     1981 attcccatcc gtcagggtaa atgtctggaa gccctgctgt ttgtttacag cgccctgacc
     2041 tttcttcaac tcccagtgcg cagggaattc accatcctgg tccgagataa aatcatcttc
     2101 aaaaattatt ttatctccgg caacaaaatc gtagttctga taagttttaa tgggaggttc
     2161 ctgcgaaagg gaggcattcg gatttttctg gtttccttcc ggaacagccg gaccggatgc
     2221 cggggtgccg gatttggcac tgcctttctc ctttttcttt ttgaataaat tgccgatgcc
     2281 ctgcccggcc ttattcaggc ccttatccgc tgcaatatcc atatcattat tggcgtgcgt
     2341 ggcagcggcg tcctgtgcca cctggttcgg atcatttacc tgggcccgca cggtacaaac
     2401 agaaaggcat atatacaggc cgccggcaag gagcaatatc cttttcattt tttttcttac
     2461 aaaagaaagg ataaacaaag ggcaagacaa tcggatgaaa atatgatatt ggagacccgg
     2521 aaccggcttt ggtttatttt gatcccggca aaagggcaaa caggttttcg ggctcgttgg
     2581 tggtgatata gcccacctgg cgggccagaa aatattccat ttcaggaacg gtatccaccg
     2641 tccagacatt caagtccagt ccggcatcca gcgctccttt gatccattgc tcctccttat
     2701 ggtaaacccg gtattgatag tccattcccc gaataccatc ttttttcagg atatccggcg
     2761 ccttatctcc attcaggtac aaggtaaccg cagcggggtc aagccggagc acttctttta
     2821 atgcatcata atcaaagctg atatagatca cccaggcctg ggcctgttcc cggtgaacca
     2881 tctccaccac cctttttgcg agctccagca catgcgtctg atcaattacc gatggtttga
     2941 tttcagcaat caaacgggtt ttctcctgct ccattcctgt tttcaggtaa tgttcgaagg
     3001 ttggcagcgc ctccccgttt tgcagcggat atttcagaag atcagtatag accgtgtgct
     3061 caattcccat cccttcatag gtcggatcgt gattgatcac gagtatgctg tctgcggtgg
     3121 actggatatc gaattcactc ccggcgcatc ccatccggat ggcggcctgc agggaagcaa
     3181 tcgagttctg gggcaggccg gtatttttcc aggccccgcg gtgagcgatc accttgtttg
     3241 ccgcaaaatt gtctttcaat atcgtaaatg tttgctccat tcccgttttg ttcccggagg
     3301 agaaggtgat cgaaaaagaa gcccggctga attttttgaa gtactttttg ccctggccgc
     3361 tcagcagaat gttattgccc tccagcttaa ataaaccggc attggagccg gccagccgga
     3421 gcgtagcctg ctgtacacta tcccccagca cccggaatag aacagcctgc ccgcgcccgg
     3481 cagaaaaggt atagttactt aaataaaccg gatagggagc cggctgccgg ccaatggaaa
     3541 tatccggacg atataaaaag gccaggatga acccgattcg gacccagcgt ttgttcatgc
     3601 gcgtaatttt ttaaagaata ccaaagtaag cgattattcc acactttcat cagcatcggc
     3661 ttccgcaatc aatggaatca ccggccggaa atggatataa aaaaacctgt tgaacggtcc
     3721 ggtcggtgaa tacacttagg aaagatattt cacagtattc taacatcttt atatcatccc
     3781 cggttaggga aatctttatt tttaacgacc caacatttcg aaacctgctt tttcatgaga
     3841 ataaccctga taataaccgc tttatttttt ctgtgccagc cattattctc ccagcaggca
     3901 tcaataaaag gattggtttc agatacctca gaacaggctt cgctccggca gagcagcatc
     3961 atgatccttc agcgcaagga ttctttcctg atcgctttta cgagggcaga cgcccgcgga
     4021 gcattctcct ttcttcatct gccggccggc ccggtgatct gcaagatctc ctatccgggt
     4081 tatgcggatt acgtcacgga actcgatctg aaggattcca cagaaacaaa cctcggcaca
     4141 gttcccatga ccctgaaaag caaactgctc caggaagtga tcgtacatgg gaatatggcg
     4201 gcgatccgga tgaaaggaga taccctcgaa tttaaagcag acagtttcaa agtgcagcag
     4261 ggagcaaccg tagaggaatt actcaagcag ttgcccggaa tacaggttga cagaaacggg
     4321 aagatcaccg cgcagggtga aacggtcaaa aaagtgctcg tggacgggga agaatttttc
     4381 ggtgacgatc cgacccttgt cacgcagaat ttacgggcgg atatggtgga caaagtacag
     4441 gtatacgata agaaaagcga ccaggccgca tttaccggaa ttgacgacgg agaacggaat
     4501 aaaaccatca atctccagct taaggaggat aaaaagaaag gatatttcgg gaaactggag
     4561 gccggcgccg gcaccaatgg gttctataac aaccaggcca tgttcaacct gtttaaaaag
     4621 aaactcaagt tttccggtta cgggatcctg tcaaatatcg gaacgatcgg attgaactgg
     4681 caggataatg acagttatgg acaaagtttc gccagcagtg tggattatga tgaaacctcg
     4741 ggctatttca gtttcaacgg caacggggac gacctggaat cctggaacgg tcagtacaat
     4801 gcccagggat tgcccaccgt taaaacggcc ggcctccatt ttaataataa gtggaaccag
     4861 gataaacaat ccgttaacct gaattataaa atattggacc tggacgtgac cggaaaaaac
     4921 gaatcccgtt cccagtattt actgcccgat accctctatt acaataacca gacggaatct
     4981 ttccacaacc atattctgcg gaacaaggcc tccggaattt atgaattcaa cctggattcc
     5041 agctcctcgc tcaagatcaa tgtggatgcc aacaaggatc ataaaaccac gcagagtaat
     5101 ttcaccagcg aataccgggc ggaagacagc agcctggtga accggaacaa ccggaatgtc
     5161 ggtacgaccg gagatgagga aaagctgaac accaatctgc tttggcggaa aaagtttcac
     5221 cggaagggac gaacgctgtc cttcaatttc cgggagaatt acaagcagaa taacagtaca
     5281 ggttacctgt attcggacac ccggtttttc ggcagggaca gcctcaatgc cgatcagctg
     5341 acggatcagt ataagacctt tttgaataaa acactaaccc tggattccag gctggcgtat
     5401 accgaacccc tgtctgaaat ttcaagtatc gcggtcaatt acgggatttc cggagggaac
     5461 agccagtcgt accggaactc ctttaataaa tccacgggcg gaaaatacga tgccctggac
     5521 agcgggtata gcaacagcta ccatttttcc ctgctctctc aaaagaccgg tctgctgtac
     5581 aattttaccg gtcgcaagct gcgtttcaat gtgggtgccg atgtcgggtt taccggattc
     5641 gatcagaaga acatggataa tggaaccaca ctccacaggc attttgtgaa ctggtacccg
     5701 caaagccgga tctcctacaa atttgcccag gagaaatctt tattcttccg gtattccggc
     5761 aatacgcagc aacccggcat tgaacaactg caacccctgg ccaataacca ggatccgtta
     5821 aatatttatg tcggcaatcc cgacctgaaa ccggccttcg tgaacagctt cgggctgaat
     5881 tattttaatt acaaaccgct cagcgaaacc gggctgttta tcaatatgta tggaaatgcc
     5941 acctccaacg cgatcagctc aaaagattat gtggacagcc ttggaagaag ggtttatcaa
     6001 tcggttaatg taaatggaaa ttattcattg aacggaaata tcaactacga catcaagctg
     6061 aaaaaatccg aaatcaatat cggcctgggt accggctttg gcgcaaaccg gtataaaaat
     6121 attgtcaacg atgaatcgaa tgaaaccaac cagaaaaact attccttcag tttacgcgca
     6181 ggaaaatcaa aacaaaagaa atacgatatc agcctgtccg gttcagccaa ttatactacc
     6241 agcatctcct ccattcagca agccatcaat acccatttct ggtctttcga attctctccc
     6301 gacctcggtc tctatttacc gttgaaattt gaactgcata caaactgcga attcaatatc
     6361 cggcagaaaa cgacggcctt cgataccaat accaatactg ccctgtggaa tggctggatt
     6421 ggcaagaagt tctttaaaca ggaatccctg ttattgaaat tacaggccaa tgatatcctg
     6481 gatcagaata tcggttttaa ccggacggta aacagcaact atatttccca aagcacctat
     6541 accacgatca gaagatattt tatgttctcc gtggtatgga atttcactaa agccggcgct
     6601 acgccatcca aaccttaata tgcgaaacgt attttgcttt atcccgttgc tgctcttctg
     6661 ttatttggag agcaatgccc agcaggctat ttttttatcc gagggaaagg tagaatttac
     6721 ccgaacggtc aatttatacg cgcggatgaa taacgacgat gaatctttca gcgaactgct
     6781 taaaaagtcc atgcccaaat tcaagaagac ctattttcag cttttctttt ccaataatca
     6841 atggatttat aaaccgggaa gagaaaaccc ggagaacaac aggctttggg aagaaccggc
     6901 agaaaataat attgtgagtg tagactatac caccgggaaa tccgtaagtc agaaagaaat
     6961 attcggggaa atgtttttgg ttcaggatag cctgagaaga attaaatgga aaatgaccga
     7021 tgaaacccgg gtcattgccg ggttcaactg ccaccgcgca aatgccctga ttatggattc
     7081 catttatgta gtcgcttttt ataccgatga gatcatcaca ccgggcggac cggaatc
//
LOCUS       DASYCU010000487         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_250750, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000487 DASYCU010000000
VERSION     DASYCU010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250750"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /gene="fucI"
                     /locus_tag="VGM24_09805"
     CDS             complement(<1..258)
                     /gene="fucI"
                     /locus_tag="VGM24_09805"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616404.1"
                     /note="catalyzes the conversion of the aldose L-fucose
                     into the corresponding ketose L-fuculose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEY4155715.1"
                     /translation="MSHQNDYPKIGIRPIIDGRLGGVRESLEQTTMKMAENVAAFYEK
                     NLKYPDGTPVKCVIADSCIGGVAEAARCAKKMESENAGVSLS"
     gene            complement(402..1568)
                     /locus_tag="VGM24_09810"
     CDS             complement(402..1568)
                     /locus_tag="VGM24_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEY4155716.1"
                     /translation="MKTKNFIYLFIGLCIFSGAMGQEKFNGIDANMGNIFRMSDAKTR
                     SISPENFKGEKGKGGMAVEGTGKSASRDLGQGWKVSPSVVIKAGTTFTVAEMDGPGSI
                     QHIWMTPTGVWRNSILRFYWDDEKTPSVEVPVGDFFGMGWGQYAPLQSLAVCVNPGSA
                     FNCYWPMPFRKKCRITMENIDEKDMVLYYQVDYILTQVPSDAAYFHAQFRRTNPLPYK
                     GVYTLVDGIKGRGQYVGTYIAIGVHNNGWWGEGEIKFFMDGDTDYPTVCGTGTEDYFC
                     GSYDFDTRKKKPGGEETSEYTEFSTPYTGLHQVIRGDGHYSVSQRFGMYRWHITDPVR
                     FDKDLKVTIQALGWRSGGRYLPLQDDIASTVFWYQAEPHAPFPVLPGKDALEVN"
     gene            complement(1695..3107)
                     /locus_tag="VGM24_09815"
     CDS             complement(1695..3107)
                     /locus_tag="VGM24_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155717.1"
                     /translation="MRILVFLSVIVLSSCKKDFLDVVPVDRIPKEEFYKTEADLTAAI
                     YGAYAAQRGMYTGGELPLYDLEETRSDNTNENLGRQTEHKAVDNFTVQSGNTSITGEW
                     GANYDCINLCNAIIDRAPNVDMDETRKQQMIGEALFIRAHAYFLLVQDYGGLPLRLHE
                     TVSLSGDNNLARSSVDSVYLQIISDLQAAASSLPDSYSGTDVGRATAYAANALLGKVE
                     LQKGDKQAAVDALRKVVFPGTPYSLLPNYADLWNPTTKNSPESIFELQFLPPLNGSPF
                     WNYFAPATLAIPGGTNGSTAPNTPTQDLINAYETNDTRLAASIGYDPGGTPYILKFKD
                     PSVSVGNDANNNFPVLRYADALLMLAEALGESTEAYDLINEVRARAHLGPVGAATPGT
                     FIDKLMHERQVELAFECHRWHDLLRLDQSETLSIMNAHLAREFPANNIVIDAHNLLAP
                     LPDAEVQTNALAEQNPGYVH"
     gene            complement(3156..>3672)
                     /locus_tag="VGM24_09820"
     CDS             complement(3156..>3672)
                     /locus_tag="VGM24_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155718.1"
                     /translation="WLYGINTNVRYKNLRLSVLLQGQAGAKMLDFVYQIMSLHGNNTN
                     MDSYFFNGRYISESQPGNGKVPRAGYNDIGAVSSWEVQSTDYLRIRNVNLSYTFPAAI
                     SNKIWLNDLRAFISVENLFTFKKYEGGNPQASRNGFDTQIFGDHRTLGLNSVATAPIP
                     RIYTLGINFSF"
BASE COUNT          887 a          942 c          866 g          977 t
ORIGIN      
        1 ggataaggac acaccggcat tttcggattc catttttttc gcacaacggg ctgcttcggc
       61 aactccgccg atgcaggagt ctgcaatcac acacttcacg ggggttccat caggatattt
      121 taaatttttc tcgtaaaagg cggccacatt ctccgccatt ttcatcgtgg tttgttcaag
      181 agattccctg acacctccca gacgcccatc aataatgggt cttataccta ttttgggata
      241 atcattctga tggctcatcg gagcggggtt tgttaaatga aaaatatttt tgtgttggct
      301 tattttcttt cttcttttcc catttaaagg tggcattcag caccgctccc ccggaccatc
      361 tgaatcatgc cttggagagt tcctgcttta ccatagacgg cctagttaac ttccagtgca
      421 tcttttccag gcaagacagg aaagggcgcg tgcggctcag cctggtacca gaaaacggtt
      481 gaagcaatat catcctgcaa cggcaaataa cgtccccccg aacgccagcc caacgcctgg
      541 atggtcactt tcaggtcttt gtcaaaacgg accggatcgg taatatgcca gcggtacatt
      601 ccaaatcttt gcgacacgct gtaatgtccg tcgccccgga tcacctgatg aagccccgtg
      661 tatggagtag aaaattccgt gtattcgctt gtttcttctc caccgggttt ctttttcctg
      721 gtatcaaaat cataagaccc gcagaaatag tcctcggtac ccgttccgca aacggtcgga
      781 taatcggtat cgccatccat gaagaatttt atttcaccct ctccccacca gccgttatta
      841 tgtactccaa tggcgatata cgttccaacg tattgacccc tgccttttat tccatccacc
      901 agtgtataaa cgcccttgta gggaagcggg tttgttctgc ggaattgcgc gtgaaaatag
      961 gctgcgtccg agggtacctg ggtcagtata tagtccacct gataatacag caccatatcc
     1021 ttctcatcaa tattctccat ggtgatccgg cattttttcc ggaacggcat tggccagtaa
     1081 cagttgaacg cactgcccgg atttacgcaa acggccagcg attgcagcgg ggcatattgg
     1141 ccccagccca tgccaaaaaa atcaccgacc ggcacttcca ccgagggcgt tttttcatca
     1201 tcccagtaaa aacgaagaat tgaattgcgc caaacgccgg tcggtgtcat ccagatatgc
     1261 tggatagaac ctggtccgtc catttccgct acggtaaacg tggtgccggc tttaatgacc
     1321 acactcgggc tcactttcca tccctgaccc agatcgcgcg aagccgattt accggtacct
     1381 tccactgcca tgccgccctt ccccttttcg cctttaaaat tttccggact gatggatcgc
     1441 gtttttgcat cggacatccg gaatatattg cccatattgg catcgatacc attaaatttc
     1501 tcctgaccca ttgcgccgga aaaaatgcag aggccaataa agaggtaaat gaaattcttt
     1561 gttttcatgc tataaattta aatattgaag cgtttccgtt gagcattgcc aaaccggaat
     1621 gccggcaatt attcagaaaa ttaggacttt agcttccatt ctcctgcatt tcctgataaa
     1681 atgagccatt ctttttaatg cacatatccc ggattctgtt ccgccagggc attcgtttga
     1741 acttccgcat ccggaagcgg ggccagcagg ttgtgggcat cgataacgat gttgttagcc
     1801 ggaaattccc tggccaggtg cgcattcata atgctgagcg tttcggattg atcaagcctg
     1861 agcaaatcgt gccatctatg gcactcaaag gccagctcaa cctggcgttc atgcattagt
     1921 ttgtcaataa atgttccggg ggttgctgcg ccgacaggtc ccagatgcgc cctggctctc
     1981 acttcattga tcagatcgta ggcctcggtg gattctccca atgcttctgc cagcatcaac
     2041 agggcatctg cataccggag taccgggaaa ttgttgtttg catcgttgcc tacagacaca
     2101 gacggatcct taaacttgag gatatacggc gtgccgcccg ggtcgtatcc gatggaagcg
     2161 gcaagcctcg tatcattggt ctcataagca ttgatcaggt cctgcgtcgg cgtgttgggt
     2221 gccgtgctgc cgttggtgcc tccgggaatc gccagcgtgg ccggcgcaaa gtaattccag
     2281 aatggtgaac catttaaagg aggcaggaat tgaagttcaa aaatggattc gggtgaattt
     2341 ttggttgtcg ggttccagag gtcggcatag ttgggtaaaa gagaataagg cgttccggga
     2401 aaaaccactt tacgcaaggc atccacggct gcctgcttgt ctcctttttg caattccact
     2461 ttgcccagca gggcgttggc tgcataagca gttgcccgtc caacatcagt accggaataa
     2521 ctatccggaa gactggaggc ggcagcctgc agatcactga tgatttggag atatacggag
     2581 tcgacagatg acctcgccag gttgttgtct ccgctcaggg agacggtttc atgcaaacgc
     2641 aggggcaggc cgccataatc ctgaacaagc agaaaatagg catgggcgcg gataaacaaa
     2701 gcttcgccaa tcatttgctg cttccgggtt tcgtccatat ccacattggg agcccggtcg
     2761 ataatggcat tgcataaatt gatacaatcg tagttggcgc cccactctcc ggtaatggac
     2821 gtgttgccgg attgaacggt aaaattatcc accgccttat gttctgtttg acgcccgaga
     2881 ttttcattcg tattatccga ccgggtttcc tccagatcat acaatggaag ttccccgccc
     2941 gtgtacattc cgcgctgtgc ggcataagca ccataaatgg ctgccgtaag gtctgcttcg
     3001 gtcttataga actcctcttt gggaatccgg tcgaccggca ccacgtccag aaaatctttt
     3061 ttgcaggatg acaggacgat aacagacagg aaaacgagta tccgcatcct gcttgaaaat
     3121 ttattttgat aaaacatcgt gatcgatttt acaggttaaa aggagaaatt gattcccagc
     3181 gtatagatcc tggggatggg cgccgtggcc accgaattca atcccagtgt tctgtggtct
     3241 ccgaaaatct gcgtgtcaaa tccattccgg cttgcctgcg gattcccgcc ctcatatttt
     3301 ttgaaggtga aaagattttc cacggaaatg aaggcgcgaa gatcattcag ccagatcttg
     3361 ttactgatgg ccgcaggaaa agtataggaa aggttcacat tccggatcct taagtaatcg
     3421 gttgactgca cttcccagga tgaaacggct ccaatatcat tgtaacccgc ccggggcact
     3481 ttcccgttac ccggttgcga ttcggatata tatcttccat tgaagaaata gctgtccata
     3541 ttcgtattat ttccatgaag gctcatgatc tggtaaacaa aatccagcat tttggcgccg
     3601 gcctggccct gcagtaatac ggacaggcga aggtttttat atctcacatt tgtattgatc
     3661 ccatacagcc ag
//
LOCUS       DASYCU010000488         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5181182, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000488 DASYCU010000000
VERSION     DASYCU010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5181182"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VGM24_09825"
     CDS             complement(<1..283)
                     /locus_tag="VGM24_09825"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HEY4155719.1"
                     /translation="MQEQNLKTHTRYVPVYHFVLWGISLAIILLSVYNLVSALESGSG
                     FLQALLLALMAVALLINSVLVRSFPLRAQDRAIRAEENLRYFSLTGKLLD"
     gene            complement(360..803)
                     /locus_tag="VGM24_09830"
     CDS             complement(360..803)
                     /locus_tag="VGM24_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4155720.1"
                     /translation="MNPNSVEVLLVEDNVDDAELTIRALRKHRMANNLFHVRDGEEAL
                     DFIFGEGKYASTRDLLCRPKVVLLDIQMPKINGIEVLQKVKSDPRTKAIPVVILTSSK
                     EDPDIQKCYVLGANGYIVKPVNFEGFAEAIRNLGYYWLLLNQQPA"
     gene            complement(831..1403)
                     /locus_tag="VGM24_09835"
     CDS             complement(831..1403)
                     /locus_tag="VGM24_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEY4155721.1"
                     /translation="MIKFHTNHFLNIRCLTAAVLLISSLALPAQTAVSREYQIKAVFI
                     FNFTQFVDWPSTSFSSGDAPLVIGVLGENPFGSYLVEIVSGEKVNGHPVLIQHFDRAE
                     EIKTCHILLVGWSDSKRSEQVISALKERSILTISDQPRFLIQGGMVKLFTKNSKVRFE
                     INPEAAKAAGLVLSSKLLRLAEIFDPAKNR"
     gene            complement(1437..>1761)
                     /locus_tag="VGM24_09840"
     CDS             complement(1437..>1761)
                     /locus_tag="VGM24_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155722.1"
                     /translation="TALSPAVLPGADIFEGVDPHHQAMLQSVVDLPEHIQFDVNARFV
                     DSLTIWPLTPAVPQYFSLDLRVAWQYKQFEFSVVGKNLLAARHVEFVSSQIPRSIYGR
                     IVFRF"
BASE COUNT          465 a          435 c          420 g          441 t
ORIGIN      
        1 tgtcgagcaa tttgcctgtt aaagaaaaat atcttagatt ttcttcggcc cggatggccc
       61 ggtcctgggc ccggagagga aagctgcgta ctaaaacgga attgatcaat aaggcgacgg
      121 ccatcagggc aagcaacagc gcctgcagga atccgctgcc gctttccaat gcggaaacaa
      181 gattatagac cgagagaaga atgatggcaa gggaaatgcc ccagagaacg aaatggtata
      241 ccggtacata ccgggtatgg gtttttaaat tctgttcctg cataaaatag gtgcttattt
      301 acaggccgga aattacgctt ttatacctgt gaaatctcca aatcacggaa tattcccggt
      361 cacgccggtt gctggtttag taacagccaa tagtaaccga ggtttcggat ggcctctgcg
      421 aagccttcaa aatttaccgg tttcacgata tacccgttgg cacccagaac ataacatttc
      481 tgaatgtccg gatcttcctt ggaggaggtc aggatcacca cggggatcgc ctttgtacgc
      541 ggatccgatt tcactttctg caacacttca atcccattta ttttgggcat ctgaatgtcc
      601 agcaggacca ccttcggccg gcaaagcaaa tccctcgtgc tggcatattt tccttctcca
      661 aaaataaaat caagcgcctc ctcaccgtcc cggacatgaa acaggttgtt tgccatccgg
      721 tgttttctta atgcccggat agtgagctcc gcgtcatcca cattgtcttc cacgagcaat
      781 acttctacgg aattggggtt cattttttaa agatttagga tacaatatag ctatctgttt
      841 tttgccggat caaagatttc cgccagcctc agcaactttg aactcaggac caggcccgct
      901 gctttggccg cttccggatt gatttcaaat cgcaccttac tgtttttcgt aaataatttt
      961 accattccac cctgaattaa aaagcgcggc tggtcgctta tggtcagaat gcttctttct
     1021 tttaatgcgg aaataacctg ctccgacctt tttgaatcgg accagccgac caggaggata
     1081 tggcaggttt taatctcctc tgcccggtca aagtgctgga taagcaccgg atgcccgttt
     1141 actttttcac cggaaacgat ttcaaccaga taagatccaa aggggttttc tcccaggacc
     1201 ccgatgacca gcggggcatc cccggaagaa aaagaggtgg acggccaatc cacaaactgg
     1261 gtgaagttga aaataaaaac ggcttttatc tggtattccc ggctgacggc tgtttgagcc
     1321 ggcaatgcca ggctgctgat caataagacg gcagcagtga ggcaccggat attcagaaaa
     1381 tgatttgtat gaaattttat caaccttaaa ttttatgagt gggtataatc tgacttttaa
     1441 aaccggaaca cgattctccc gtagatgctg cggggaatct gcgatgatac gaattccaca
     1501 tggcgcgcgg ccaaaagatt tttaccgacg actgaaaatt caaactgctt atactgccag
     1561 gcaacccgca ggtccaggga aaaatattgg ggaacggccg gcgtgagcgg ccagatcgtt
     1621 agagaatcca cgaatcgtgc gttcacatca aactggatat gctccggcag atcgaccacc
     1681 gactgcagca tggcctggtg gtgcggatcc accccttcaa agatatcggc gcccgggagc
     1741 accgccggac taagtgcggt a
//
LOCUS       DASYCU010000489         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2280073, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000489 DASYCU010000000
VERSION     DASYCU010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280073"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGM24_09845"
     CDS             complement(<1..528)
                     /locus_tag="VGM24_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155723.1"
                     /translation="MVQFIKTVARHVIFLMLPALGFAQSTFLPEGSKYDHFLDRLGIL
                     LQSNPDLNVFTPKPLSRKVAVGVAEMADSIEQASAADERHRLSNTDLDNLHSLLMNNS
                     EWATGSKAGFESKHPLWNTIYKTKANFLEVNQRDFFLAINPVIQQQQSSESGNNEHVF
                     LNSKGLTMRGLIGNHL"
     gene            558..1949
                     /locus_tag="VGM24_09850"
     CDS             558..1949
                     /locus_tag="VGM24_09850"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY4155724.1"
                     /translation="MQNYLIKNISIVNEGRIFNSDLLIRDGRIEKIGNSLSTPSAVSE
                     INGEGLHLLPGCIDDQVHFREPGLTHKADIHSESRAAVAGGVTSFMEMPNTLPPAFTQ
                     ELLEEKYLIGERTSLANYSFYMGTSNTNAEEVLKTNAKKNEICGIKIFMGSSTGNLLV
                     DDFGTLNQLFRGCELLIATHCEDEQIIRRNLQAFRSAGRELTAADHPLIRSEEACYTS
                     SRAAAELAKKYNSRLHIFHLSTEEELELFTNTVPLEQKRITAEVCVHHLHFTADDYPR
                     LGNLIKCNPAIKAARHRDALWKALLDDRLDLIATDHAPHTLQEKGYFRNPAGRLEKIA
                     GPAGAYLEAHAGLPLVQHPLLIMLHYVKSGRISLEKIVEKMSHAVAACFQIRDRGFIR
                     EGYFADLVMVDLQASSDIHAGNILYKCGWSPLEGMHLPASIVNTFVNGHKVYGNGVFD
                     ESQKGKRLTFQRQ"
     gene            1971..3290
                     /locus_tag="VGM24_09855"
     CDS             1971..3290
                     /locus_tag="VGM24_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY4155725.1"
                     /translation="MQIDQTTYDDLSVFETAGEASLFNKLDHTRTVGGRTQLYQFFIQ
                     PFSNLEKIAEVQQILQLILRNEEAWGSSISNGTVMVMERFYETAIDIPGSYDPVNAFI
                     YKVFHAPDYSLTRYSIGHFADFLRGMRSIAALAGNADCPPVLQSLLQRVERLLHQDVL
                     RDLMNKTPGKKYSHGEVVQYGYFIRNRFKTAALDLIRIYGKLDAWYSMAMAGKRFNLV
                     TPEFLPAADPMVEVTGLYHLLLSDPVSYQLRMDPGSNFVFLTGANMAGKSTFIKAVGC
                     SLFLAHLGMGVPATRMRLSLFDGILSNINVVDNIAKGESYFYNEVQRIKNTIVQISDR
                     KKWLVLIDELFKGTNVEDSMKCSSVVIRGLIKITTSLFILSTHLYEIAGELKTFPNIS
                     FKFFETRVNNDQLEFNYQLRDGVSTDRLGYLILKREGVVALLEKPAP"
BASE COUNT          870 a          920 c          894 g          833 t
ORIGIN      
        1 gaggtgattg ccaatgagcc cgcgcatcgt caggcctttg gaattcagga atacgtgttc
       61 attatttcct gactcgctgg actgctgctg ctgaatgacc gggttaatgg ccagaaaaaa
      121 atccctttga ttgacttcca gaaaattggc ctttgtttta tagatcgtat tccacaacgg
      181 atgtttgctt tcgaaaccgg ctttgctccc cgttgcccat tcgctgttgt tcatcagcag
      241 gctgtgcagg ttatccagat cggtgttgga caatcgatgc cgctcgtccg ctgccgatgc
      301 ctgttcaatg gagtcggcca tttccgcgac gcccacggcc actttccggc tgagtggttt
      361 cggcgtaaaa acgttcagat cggggttgct ttgaagaagg atgccgagac ggtccagaaa
      421 atgatcatat ttggatcctt caggaaggaa ggtcgactgc gcaaaaccaa gggccggcag
      481 catcagaaat atcacatgcc ttgccacggt tttaataaat tgcaccatgt aagctgttta
      541 cgatttcgat ccaagatatg caaaattacc tgataaagaa tatttccatc gtcaatgaag
      601 gccgcatatt taacagcgat ctgctgatcc gcgacggacg aattgagaag atcggcaaca
      661 gcctgtccac accgtccgcc gtttcagaaa tcaatggaga ggggctccat ctgctgcccg
      721 gctgcatcga cgatcaggtg catttccggg aacccggact cacgcacaag gcagatatcc
      781 acagcgaatc ccgggccgcc gtggccggcg gtgtcacttc cttcatggaa atgcccaata
      841 ccctgccacc cgcattcacg caggaactgc tggaagaaaa gtatctgatc ggggaaagaa
      901 cttcgctggc caattactca ttttacatgg gtacgtcgaa tacgaatgcg gaagaagtgt
      961 tgaagacgaa tgcgaaaaaa aacgaaatct gcggaatcaa gatattcatg ggttcttcca
     1021 cgggcaatct gctggtcgat gatttcggaa ccctcaatca attgtttcgc ggatgcgaat
     1081 tgctgattgc cacccattgc gaagatgaac agatcatccg ccgcaacctg caggctttcc
     1141 ggtctgccgg acgggaactg accgccgcag accatcccct gatccgcagc gaagaagcct
     1201 gctatacttc gtcccgcgct gcagctgaac tggcaaaaaa atacaatagc cgcctgcaca
     1261 tcttccattt gtcaacggaa gaggaactgg agcttttcac caacaccgta ccgctggagc
     1321 aaaagcggat tacggcggaa gtctgcgtgc atcatctgca ttttaccgcg gatgattacc
     1381 cccggttggg aaacctgatc aagtgcaatc cggccatcaa ggcagctcgg caccgggatg
     1441 ccttgtggaa ggccctgctg gacgaccgtc tcgatctgat tgccacggat catgctcccc
     1501 acaccctgca ggagaaagga tatttcagaa atcccgcggg ccggctggaa aaaattgcag
     1561 gaccggcagg cgcttatctg gaagcgcatg cgggattgcc gctggtgcag catcccttgc
     1621 tgataatgct gcactatgta aaatcaggac ggatttccct tgagaaaata gtcgaaaaaa
     1681 tgagccatgc cgtggctgcc tgtttccaga tccgggatcg cggatttatc cgggaggggt
     1741 atttcgccga tctggtgatg gtggatcttc aggcatcttc ggatatccat gccggtaata
     1801 tcctgtacaa atgcggctgg agcccgctcg aaggcatgca tcttccggct tccatcgtaa
     1861 atacttttgt gaacggccat aaagtttatg gaaatggggt gtttgatgaa tctcagaaag
     1921 gtaaaaggct gacctttcaa aggcagtgat ttccggatca tgctctaact atgcaaatag
     1981 atcagaccac gtacgacgac ctttccgtat ttgagacagc gggagaagct tcgctgttca
     2041 acaaactgga ccatacccgg accgtgggtg gaagaaccca gttgtatcag ttctttatcc
     2101 agcccttcag caacctcgaa aagattgctg aagttcagca gattctgcag ctcattctcc
     2161 ggaatgaaga ggcctgggga tcttccatct caaacggtac cgtgatggtt atggagcgat
     2221 tctacgaaac cgccatcgat attcccggaa gttatgatcc tgtcaatgct ttcatctaca
     2281 aggtttttca cgccccggat tattccctga cgcgctattc cattggccat tttgcggatt
     2341 ttctgcgcgg catgagatcc atcgccgccc tggccgggaa tgccgattgt ccccccgtgc
     2401 tgcaaagtct gctgcagcgc gtggagagac tcctccacca ggatgtgctg cgggacctga
     2461 tgaacaagac cccgggcaaa aaatacagtc acggagaagt ggttcagtac ggctatttta
     2521 tacggaaccg gttcaaaacc gcggcgctgg atctcatccg tatttacgga aaactcgacg
     2581 cctggtattc catggccatg gccggaaaac ggttcaacct cgtgacgcct gaattcctgc
     2641 ctgccgccga tcccatggta gaggtcaccg gcctgtatca tctcctgctc agcgaccccg
     2701 tttcctatca gctgaggatg gatcccggaa gcaactttgt ttttcttacc ggcgccaaca
     2761 tggccggcaa aagcacattt atcaaggccg tcggttgctc cctgttcctg gcccacctcg
     2821 gcatgggcgt tcccgcgacc cgtatgcggc tgagcctttt cgacgggatt ctcagcaata
     2881 tcaatgtggt cgacaatatc gcaaagggag aaagttattt ctacaatgaa gtgcaaagaa
     2941 taaaaaacac cattgtccag atcagcgaca gaaaaaaatg gctggtgctg attgatgaat
     3001 tgttcaaggg cacgaatgtg gaagactcca tgaaatgttc gtcggtggtt atccgcggac
     3061 tgattaagat cacgacctcg ctttttatcc tgtccaccca cctgtatgaa atcgccggag
     3121 agctaaagac ctttccgaac atatccttca agttttttga aacccgggtc aacaacgatc
     3181 agctggaatt caactatcag ctgagggatg gtgtcagtac cgaccggctg ggttacctga
     3241 tcctgaaacg ggaaggggtg gttgcgctgc tggaaaaacc ggcgccttaa aagccggaag
     3301 cgattatctt tgatgccgta atgctccggt tcatcaccaa aacgggtcgt atgcaaaagc
     3361 cattttcatt cctggtcctg acatccttgt cagccttttt tcttttttcc tgcggaacac
     3421 cggccgcgcc cgcaaaaacg gacgaccgta ccgaaaaaga ttccctggcc atggcgcctt
     3481 cgataaaaac ggaggaagtg ctttatacgg ccgacag
//
LOCUS       DASYCU010000490         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5181670, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000490 DASYCU010000000
VERSION     DASYCU010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5181670"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VGM24_09860"
     CDS             <1..445
                     /locus_tag="VGM24_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155726.1"
                     /translation="EKVLGTIRFPGLLYKPIRSVYYTLKGNIAMMKQDFDGAEKNMRK
                     GLDLGMPMKEAEGASLLQMGMLAMQKNDIRKAEGLIRQALSKGLPDKENQAAAYLQLC
                     SIMMTKREFRAAKDYFKKAKNLKPSTQQIVDQIKQIEKYISRIPG"
     gene            complement(449..826)
                     /locus_tag="VGM24_09865"
     CDS             complement(449..826)
                     /locus_tag="VGM24_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4155727.1"
                     /translation="MYSQASPGIFIVEDNLLNQQLIARELEAFGGRIFFYTAGEACLA
                     DLGRHPSIVVLDYNLDGKINGLETLEEIRKFDPAIFVVLFSSQQEVHSTENLERYGSF
                     DFLEKREQSFSLLKQLVKAKMCW"
     gene            complement(1146..1916)
                     /locus_tag="VGM24_09870"
     CDS             complement(1146..1916)
                     /locus_tag="VGM24_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4155728.1"
                     /translation="MYNPRTEKRLYGKTILVAEDVELNQHLTRHILESSGASVVIANN
                     GSEALDWLKRQYFDCILMDVQMPEMDGIEATEQIRRLPDPVMSATPIIALTANCHAED
                     LLKYQRAGMDDFLAKPFDEALLLSAILSNAKKKKEVISESPGQDLYDLTMIRSVSGGD
                     PAFIKKMIQLFIETVPQNVKELVDSTLAGNWEQVSKMAHKLKSTVDSMGIKSIHADIR
                     KVEANAKTGENLDQIPNLVKEVEAVVSLCIRQLQKELS"
     gene            complement(1954..>2545)
                     /locus_tag="VGM24_09875"
     CDS             complement(1954..>2545)
                     /locus_tag="VGM24_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY4155729.1"
                     /translation="QAKLLRVLQEREITRIGANAVMPINVRIIVATHKNLLDEVKNKT
                     FREDLYYRLVGLPIHLPPLRERGTDIAVLAKSFVDNFCKENKIARKVLTPEAQQKLTG
                     YPFPGNVRELKSIMELAVVMSDGESILPEHITLNASTSISDLLNRETTLKEFESQIIQ
                     HYLDKYDKDVLLVARKLDIGKSTIYRMIQNGELKSK"
BASE COUNT          627 a          643 c          631 g          644 t
ORIGIN      
        1 tgaaaaagta ctcggcacta tccgctttcc cggcctgctc tacaagccca tccgctccgt
       61 atattatact ctcaagggaa atatcgccat gatgaagcag gactttgacg gagcggaaaa
      121 gaatatgcgg aagggcctcg acctgggcat gccgatgaaa gaagcggagg gcgcaagcct
      181 cctgcagatg gggatgctcg ccatgcagaa aaacgatatc cggaaggcgg aagggcttat
      241 ccgccaggcc ctcagcaagg gactgccgga caaagaaaac caggctgcag cttacctcca
      301 gctttgttcc atcatgatga ccaaaaggga attccgggcg gcaaaagact acttcaaaaa
      361 agcgaagaat ttaaagccca gcacccagca gatcgtggac cagatcaaac agatagagaa
      421 gtatatttca aggattccgg gctgattcct accagcacat ctttgctttc accagctgct
      481 tcagcaggga aaaggattgt tctctttttt ccaggaaatc aaaactgccg tagcgttcca
      541 ggttctccgt actgtgtacc tcctgctggc tggaaaataa aacgacaaat atcgcggggt
      601 caaatttgcg gatctcttcc agggtctcca acccgtttat ctttccatcc aggttataat
      661 ccaggacaac aatggacggg tggcgcccca gatcggccag gcaggcttcc ccggccgtat
      721 aaaaaaagat ccggccgcca aaggcctcca attcccgggc gatcagctgc tggttcagca
      781 gattgtcctc tacaataaat atcccgggag atgcttgcga atacatgggt tagaaattat
      841 acagtatcag tacgaaaaga gggccggatt tttatcactt ataaggattt catttgcaaa
      901 taattagatc ccgacacgcg gtccaggctt tcccaatccg ggaaagtggt gcaaaagctg
      961 ggaatgacgg tttgtctggt cggagcctca gcgtggcgtg cgttcaataa ttgcagggaa
     1021 tattgcggtg aatgataaag tgaggaatca gagaatcata aacgagtgta ttccggagaa
     1081 tgataaagaa gagtaaacgg gagaatgaaa taattgggga atgaaccggc aggaaccggg
     1141 aacttctagc tgagctcttt ctgaagctgg cggatacaaa gggatacgac ggcctccacc
     1201 tcctttacca ggttcggtat ctggtcgaga ttttccccgg tcttggcatt ggcttccacc
     1261 ttccggatat cggcatggat ggatttgatc cccatggaat cgaccgtgga ttttaactta
     1321 tgcgccatct tggaaacctg ttcccagttc ccggccaggg ttgaatcaac cagctctttt
     1381 acgttctgcg gaacggtttc aataaacaat tggatcatct tttttataaa ggcaggatcg
     1441 ccgccggaga cggagcggat catcgtcaga tcatagaggt cctggcccgg tgattccgat
     1501 atcacttcct ttttcttttt tgcattgctg agaatcgcac tcagcagcag ggcttcatca
     1561 aagggtttgg ccaggaaatc atccatcccg gcgcgttggt atttgagtaa atcttccgca
     1621 tgacaattcg cggtcagcgc aatgatgggg gtggccgaca tcaccggatc cggcagccgc
     1681 cggatctgct ccgtggcttc aatgccgtcc atttccggca tctgtacatc cattaagata
     1741 caatcgaaat attggcgctt taaccagtcc agcgcttcag agccgttgtt ggcaatcacc
     1801 acagatgctc cgctggattc caggatatgc ctcgtgaggt gttgattcaa ctcgacatct
     1861 tcggcaacca atatggtttt tccatacagc ctcttttcgg tccgggggtt gtacatgggg
     1921 tttgtcttct tgatccggga aatcccggtt ttcctattta cttttcaact caccgttttg
     1981 gatcatccgg taaatagtgg atttcccaat atccagtttc cgcgctacca gcaacacgtc
     2041 cttgtcgtat ttatcgaggt aatgctgaat gatctggctt tcgaattctt ttaaggtcgt
     2101 ctcgcgattc aacagatcgc tgatgctggt agaggcattc agggtaatgt gctccggcag
     2161 gatggattcc ccatcactca tgaccaccgc cagctccatg atggatttca actcccgcac
     2221 gttccccgga aagggatacc cggtcagctt ttgctgggct tccggcgtaa gcactttcct
     2281 ggctatctta ttttccttac agaaattgtc tacgaaactt tttgccagca ccgcaatatc
     2341 ggtccctctt tcccgcaagg gcggaagatg gatcggcaaa ccgaccaggc gatagtacag
     2401 gtcttcccgg aacgttttat tctttacctc atccagtaag ttcttatgcg ttgccacgat
     2461 gatccggaca ttgatcggca ttacggcatt ggcgccgatg cgcgtgatct ctctttcctg
     2521 cagtacacga agcagcttgg cctgc
//
LOCUS       DASYCU010000491         5578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2572426, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000491 DASYCU010000000
VERSION     DASYCU010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572426"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="VGM24_09880"
     CDS             complement(<1..1708)
                     /locus_tag="VGM24_09880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY4155730.1"
                     /translation="MESFSKMKFTLFLLAAFLTFDCLAQPLPLDPAIRKGKLPNGFTY
                     YIRKNTEPAKRVQLYLVNKVGSVLEQDDQRGLAHFMEHMSFNGTRHFPKNDLVHYLQK
                     SGVRFGADLNAYTSFDETVYQLPIPTDNPALLKNGLQIMRDWAGNASLDPKEINDERG
                     VVLEEKRLGKGAQERMREKTFPVILNQSRYAERLPIGVDTVLKNFKKPTILKFYQDWY
                     RPDLQALIVVGDINVDSMEARIKQLFGDLKNPPREKPRVKYTIPLNGKNQFISVTDKE
                     FPYTVIQVLIKKKTLVAKTLAEYHQLINRELFNQMLASRIAELSQEPNPPFIQASADM
                     GQFLGGLDAFTVSVVAKPGLLEKSFISVWQLIRQLKEFGFTEPEFERAKTSYLSYFAS
                     ALREKDKRDSKDLVEEYTRNFLTGESAPGIEKEYDLTQAFLKTAKLADINGLAPVFIS
                     DSNRDIVIMAPQKDSASLPSGEIVQSWIDTVAGRKLSPYQDQFTKESLIPENLVAGKV
                     VDEKKTAPAMGLTLLTLSNGLKVVLKPTDFKNDEIRFQAFSPGGSSLYPDSDYESASS
                     AAE"
     gene            complement(1740..2999)
                     /locus_tag="VGM24_09885"
     CDS             complement(1740..2999)
                     /locus_tag="VGM24_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEY4155731.1"
                     /translation="MPKRTLAFLLSLFLIPGLALQAQTIDILLKGGHVIDPKNKIDSK
                     MDVAIRGDKIVQVAADIPAGSAKKVIDVSGLYVTPGIIDMHVHVFYGTDPGSYLANGH
                     DGVAPDAFSFRAGVTTMVDAGSSGWRNFRLFKAQTIDRSQTRVLAFLNIVGGGMYGRF
                     EEQDVTDMNPVMTANMIKKLYPDILVGIKAAHYWGDFTQVDRAVEAGNLANVPVMVDF
                     GEHHPPNSIEELFMKHLRPGDIFTHSYADGPTERETIVDDNTAKVKPFVFEARKRGII
                     FDVGHGGGAFAWKQAVPAIKQGFLPNTISSDLHTGSMNSGMKDMSNLMSKFLNMGLTL
                     EQVIERATWNPANVIKRPDLGSLSVGSDADIAVFNLLKGDFGFLDVRKTKLKGTQKLQ
                     AELTIRAGKIVWDLNGISAPLWDAKPE"
     gene            complement(3068..4693)
                     /locus_tag="VGM24_09890"
     CDS             complement(3068..4693)
                     /locus_tag="VGM24_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155732.1"
                     /translation="MKRRDVLKGLSLLPLGTTVFPIQSAMAASGIPPKTGPLVPGPEI
                     FQSIGVEPLINCMGTFTIIGGSLERTSVRTAMEAASRNFVQYDELAEGIGRRLAEVTG
                     AEWGMVSAGCAAGLKHVAAACVTGGNPEKLIRIPDLTGFDKTEVVIPKSSRNEYDHAI
                     RNIGVKVIDVETLEELENACGQRTAMIYIMSESKEPLSLESIARVAKPRNIPILVDAA
                     AEDLTIPCVHLQQGATVVAYSGGKALCGPQCSGILLGKKDILQSAWQASSPHHGPGRD
                     NKVGREEMIGALAAIEAWVTFDHKEQWKKWLSYLDTVNRKVSGIETVKTLVRNPTGLS
                     NQSPALVISWEPDKLHITGEEVAEELGRNKPRIALASGNRHNGDKGSGLTSISITAGQ
                     MQPGEDKIVADRIHGVLSQKRNPKSAEMKPPAVNMQGRWDVEVEFYSSKAPYTWTLEQ
                     DGNWIRGIQLGSFATQDILGTIEGDQVKLRSPENLPGDRITFTYSGNISGDSLSGSIY
                     LVEYGTAKFTASRHKYPEGHMPVTVPGGPPLAT"
     gene            complement(4721..>5578)
                     /locus_tag="VGM24_09895"
     CDS             complement(4721..>5578)
                     /locus_tag="VGM24_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4155733.1"
                     /translation="FALFKQQVIDQSKTRVLAFLNIAGSGMSGSPGEEDVNDMDPQMT
                     SLTIKKYPDIIVGVQIGHYNGNEWTPFDRAIEAGRLSGTPLLVECHLPQLPLKDQLNH
                     MRPGDIITHAYEKVSERMPVTDSLGKVRAFVLDAQKRGVLFDVGHGGAGFWFSVAIPA
                     LKQGLAPNSFGTDLHRFSMNAGMKSILNVMSKYLNMGMSLEDIIRRATWFPARSIKRA
                     DLGQIGEGAVADVTVLSMQHGKFGFVDAGGNRLDGDRKLEAELTIRAGKIVYDLNGIS
                     AKKLKIAED"
BASE COUNT         1240 a         1508 c         1355 g         1475 t
ORIGIN      
        1 tttcagcagc gctggaagcg gactcataat cactatccgg atacagggaa ctgcctccgg
       61 gactgaaagc ctgaaaccgg atctcgtcat ttttaaaatc cgttggtttc aaaaccactt
      121 tcaatccgtt gcttaaggtt agcagtgtta atcccatggc cggcgcggtc tttttttcat
      181 ccaccacttt tccggcaacc aggttttccg gtatcaggga ctctttggta aactggtcct
      241 ggtagggaga taatttccgc ccggcgacgg tatcgatcca gctctgaacg atttcgccgg
      301 aaggtaagga agcactgtct ttctgagggg ccatgatcac aatatcccgg ttactgtccg
      361 aaataaagac cggcgctaac ccattaatat cggccagttt tgctgttttt aaaaatgcct
      421 gggtcaaatc atattccttt tcaatgccgg gcgctgattc gccggtcaga aaattccggg
      481 tgtattcttc cacgaggtct ttcgaatcgc gcttatcctt ttctctgagg gccgatgcga
      541 aataactgag gtaactcgtc tttgcccgtt caaattcagg ttcggtaaat ccaaactcct
      601 ttagctgccg gatcagttgc catacggaga taaaactttt ctccaatagt ccgggtttgg
      661 ctaccacgga aaccgtgaat gcatccaacc ctcccaggaa ttgccccata tccgcgctgg
      721 cctgaataaa tggcgggttc ggttcctggg aaagttccgc aatccgggat gccagcatct
      781 ggttgaacag ttcccggttg atgagctggt gatattcggc cagtgttttg gcgacgaggg
      841 tctttttctt gatcaatacc tgaataacgg tatagggaaa ctctttatcg gtaacggaaa
      901 taaactgatt ctttccgttt agcggaatgg tatattttac cctgggcttt tcccggggcg
      961 gatttttgag gtccccgaat aactgtttaa tccgcgcttc catgctatcc acattaatgt
     1021 cccccaccac gatcagggct tgcagatccg gccggtacca gtcctgatag aacttcagga
     1081 tcgtcggttt tttaaagttc ttcagcacgg tatccactcc gatcggaagc cgctctgcat
     1141 accgcgactg attgagaatg accggaaacg ttttctccct cattctttcc tgcgctcctt
     1201 ttccgagcct tttttcttcc agcaccacgc cccgttcgtc attgatctct ttgggatcga
     1261 ggctggcatt tcctgcccaa tcgcgcatga tctgcaggcc gttcttaagc aaggcgggat
     1321 tatccgtcgg aatcggcaat tggtaaacgg tttcatcgaa actggtgtat gcattcagat
     1381 cggccccgaa ccggacgcct gatttttgca ggtaatgaac caggtcgttt tttggaaaat
     1441 gcctggtgcc gttaaagctc atgtgttcca taaaatgagc aaggccccgc tggtcatcct
     1501 gttccaggac tgatccgacc ttattgacca ggtataattg gacacgtttg gccggttcgg
     1561 tatttttccg gatatagtag gtaaagccat tgggcagttt gccttttctg atggcgggat
     1621 ccaggggcag gggttgggcc aggcagtcga aggttagaaa agcagctaac aaaaacaggg
     1681 tgaatttcat cttagaaaaa ctttccataa agataaagcg tatttagcag ggcaatttat
     1741 tattcgggtt tggcatccca taaaggcgcg ctgatgccgt tcagatccca aacaattttt
     1801 ccggcgcgga tcgtgagctc cgcctgcagt ttctgtgttc ccttcagttt tgtttttcgc
     1861 acgtcgagaa atccgaaatc gccttttaat aaattaaata ccgctatatc cgcatctgat
     1921 cccacactta aactgcccag gtcaggtctt ttaataacgt tggccggatt ccaggttgcc
     1981 cgttcgatga cctgctcgag ggtaagaccc atattgagaa acttggacat caggttggac
     2041 atatctttca tcccgctgtt catgctgccg gtatgcaggt ccgaactgat ggtgtttggc
     2101 aggaagccct gttttattgc cggaaccgcc tgcttccagg caaatgcgcc tccgccgtga
     2161 cccacatcga agatgatccc tcttttccgc gcttcaaata caaagggttt gacctttgcg
     2221 gtattgtcat ctacaatcgt ttcccgctcc gtgggtccgt cggcgtaaga atgggtaaaa
     2281 atatctccgg gacgaagatg tttcataaat agctcttcaa tcgaattcgg cggatgatgt
     2341 tcgccaaaat ctaccattac cggaacattc gccaggttac cggcctccac ggcgcggtcc
     2401 acctgcgtaa aatcgcccca ataatgggcc gctttaatgc cgacaagaat atccgggtag
     2461 agttttttga tcatattcgc ggtcatcacc ggattcatgt ccgtcacgtc ctgttcttca
     2521 aagcggccat acattccccc gcccacaata ttcagaaatg ccagcacgcg ggtctgcgac
     2581 cggtcgatgg tttgcgcctt gaagaggcgg aagtttctcc atcctgaaga accggcatcc
     2641 accatagtgg tcactcccgc tctgaaagag aaggcgtcgg gagcgacgcc gtcatgaccg
     2701 ttggcaagat aggaccccgg atccgtgccg taaaaaacat gcacgtgcat atcaataatg
     2761 ccgggcgtta cgtacaagcc acttacatct atcacttttt tggcgctgcc tgccgggatg
     2821 tccgcagcca cctgcacaat tttatcgccc cggatggcga cgtccatttt ggaatcaatc
     2881 ttgtttttcg gatcgatcac atgaccaccc ttcaacagga tgtcgatcgt ttgtgcctgc
     2941 agggcaagac caggtataag gaaaagggag agtaagaagg caagtgtacg tttaggcatg
     3001 atgcagatta attttgaatt tatccttttg atttattgaa aatttgcaaa ccgttgatcc
     3061 ggatttccta ggttgccagg ggtggcccgc ccggaacggt taccggcata tgaccttccg
     3121 gatatttatg acgactggcg gtgaatttgg ctgtcccata ctccaccaga tagatggagc
     3181 cggacaggga gtctccggaa atgtttccgg aataggtaaa tgtgatccgg tctcccggaa
     3241 gattctccgg acttcgcagc ttaacctggt ctccttctat tgttcccaaa atatcctggg
     3301 tggcaaaact tccgagttga atccccctta tccagttgcc gtcctgttcc aaagtccagg
     3361 tgtagggcgc tttgctgcta tagaattcca cttcgacgtc ccagcgcccc tgcatattca
     3421 ctgccggcgg tttcatttcg gcagacttcg ggtttctctt ttgagaaagc acgccgtgaa
     3481 tccggtctgc cacgatctta tcttcaccgg gttgcatctg ccctgcggtt atactgatcg
     3541 acgttaatcc ggagccttta tcgccattgt ggcggttacc ggatgccagt gcaatccggg
     3601 gcttgttcct gcccagctcc tccgccactt cctctccggt aatatgcagt ttgtccggct
     3661 cccagctgat caccaatgca gggctctgat tggagagtcc cgtcggattt cttaccaggg
     3721 tctttaccgt ttcgatgccg gaaacctttc tgttcaccgt atccaggtaa gaaagccatt
     3781 ttttccattg ttctttatga tcgaaggtca cccaggcttc gatggcagcg agtgcgccga
     3841 tcatttcttc ccggccaacc ttattgtcgc ggccgggtcc gtgatgcgga gagctggcct
     3901 gccaggcgga ttgaagaata tcttttttgc ccagcaggat gccggaacac tgcggcccgc
     3961 aaagcgcttt cccgccgctg taggcaacaa ccgttgcgcc ctgctgcagg tgtacgcagg
     4021 gaatcgtaag atcctccgcc gccgcatcaa ccaatatcgg aatgttccgg ggtttggcga
     4081 cgcgggcgat gctctccaaa gaaagcggct ctttgctttc agacataata tagatcatgg
     4141 cggtgcgctg gccacaggca ttttccaatt cttccagcgt ttccacatcg atcaccttaa
     4201 ccccgatgtt ccggatcgca tgatcatatt cgttccggga ggacttcggg atgacgactt
     4261 ccgttttatc gaatcccgtc aggtccggga tcctgataag cttttcggga tttcccccgg
     4321 taacacaggc agcggcaacg tgtttcaggc cggccgcaca accggcagag accatacccc
     4381 attcggcgcc ggtcacttcc gccagccggc ggccaatgcc ctccgccagt tcatcatact
     4441 gcacgaaatt cctggaggcc gcttccatgg cggtccgcac ggatgtccgc tccagagaac
     4501 caccgatgat ggtgaaggta cccatgcaat tgatgagggg ttcaacgccg atggactgga
     4561 atatttcggg tccgggcacc agcggaccgg tcttgggggg aatgcctgag gctgccatag
     4621 cggattggat gggaaaaaca gtcgttccca gcggcagcag ggacaatccc ttcagaacat
     4681 ctcttctttt catatgattt attattttat ttcatcgaat tcaatcctcc gcaatcttca
     4741 attttttcgc cgaaataccg ttcagatcgt aaacgatctt tccggcacgg atggttaatt
     4801 ccgcttccag cttccggtct ccgtccaggc gattgccacc cgcatccaca aaaccaaatt
     4861 ttccatgttg catgcttaat accgttacat cggcaacggc gccttcccct atctgcccga
     4921 ggtccgcgcg tttgatggac ctggccggga accaggtggc gcgccggata atatcttcga
     4981 ggctcatccc catatttaaa tatttggaca tcacattcag aatacttttc atgccggcat
     5041 tcatactgaa ccggtgaaga tccgtcccaa atgaattggg agcaagcccc tgtttcaaag
     5101 ccggtatggc tacactgaac cagaagcccg cgccaccatg ccccacatcg aagagcaccc
     5161 ctcttttttg tgcatccagc acgaaggcac gaactttacc caggctgtcc gtaaccggca
     5221 tccgttcgga aaccttttca taggcatgcg tgataatatc tcccggccgc atgtgattca
     5281 gctgatcttt cagcggcagt tgcggcagat ggcattccac cagcaagggt gtcccggata
     5341 aacgtccggc ttcaatggcc cggtcgaatg gcgtccattc atttccgtta taatggccga
     5401 tctgtacacc tacaattata tccggatact ttttaatggt cagcgaggtc atctgcggat
     5461 ccatatcatt cacatcctct tctcccggac tgccgctcat tccggagccg gcaatattga
     5521 gaaaagccag cacgcgggtt ttggactgat caatcacctg ctgtttaaaa agagcaaa
//
LOCUS       DASYCU010000492         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_395810, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000492 DASYCU010000000
VERSION     DASYCU010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395810"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1831
                     /locus_tag="VGM24_09900"
     CDS             290..1831
                     /locus_tag="VGM24_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY4155734.1"
                     /translation="MLFPLLASVAGFAQLNNTKGAPVVKTTGGMLEGVNSSGILTFKG
                     VPFAQPPTGNLRWKEPMPAKPWQGIRKADHFGPRAMQLPLFGDMNFRSDGVSEDCLYL
                     NIWTPAKSEKAQLPVLVYFYGGGLVAGDGSEYRYDGESMARKGLVAITVNYRLTVFGF
                     FSHPELTKESPHHASGNYGFLDQVAALNWVKQNISAFGGDPDKITIAGESAGSFSVSA
                     LMASPLSRNLIAGAIGESGSLLGLQHPATLEEAEKRGVEFADSQHVNSLAALRALPAE
                     QILKASAKFRRGNYPVDGYFFTRSPLDVFTAGQEAKVPLLVGWNSEEGNWQGILGKDQ
                     PTKENYDKAIQKMYGGHADEILKVYHADNDAEVESVATDLASDRFIGFSTWRWSDLQA
                     KTGGSKVYRYHYERPRPKTRDAQNTPAARGAVHSSEIEYALGNLPTNRVFDWQPEDYL
                     VSEILQAYFANFIKTGNPNGLGLPDWPAVIPDKPAAVMRVDVNTRAETEKHRDRYLAQ
                     EKLGN"
     gene            1855..>2961
                     /locus_tag="VGM24_09905"
     CDS             1855..>2961
                     /locus_tag="VGM24_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HEY4155735.1"
                     /translation="MSQINDRRQRGILQTIARRAMIDKGLLPDFSPEAITQLNGIQET
                     GAKAAGPVRDLRDLAWCSIDNDDSLDLDQLSVAVPAQGNQTQILIAIADVDAVVKKDS
                     ALDKHASQNTTSVYTAAQTFPMLPEKLSTDITSLNPDADRYAVVVEMSIGDDGALQNS
                     DIFLAVVRNRAKLAYNSLAAWLEGKGPIPEAVGKVPGLDQNIRLQDRIAQKLKSVRFA
                     NGALDFETIEARPVFEGDEIRDLQAEKKNRAKTIIEDFMIAANGIVARWLAARKFPSM
                     RRIVRTPKRWNRIVDLAAEHKFSLPQQPDSKALDQFLQLQKDSDPDRFPDLSLSIIKL
                     LGAGEYSVELPGENVSGHFGLAVKDYTHSTAPNRR"
BASE COUNT          745 a          752 c          795 g          669 t
ORIGIN      
        1 cgcttcttca ataatgccct ggatggttaa gaccttatat aaataattaa caggttcaac
       61 tggcatggca ggcaccggaa ttgattgctg agttcgttcc catgatctat ttattgctaa
      121 attaattcaa ttctcccatg atcggcccgg ccgggatttc agccgttgct cattcagata
      181 aatatattcg gacaggaaat ttcgctcccc tttaaaaaaa cttccgtttg tttgacctca
      241 tttttagtat attgaacgca gatattaact catgaaaaaa atttccgtta tgctgttccc
      301 cctgcttgcc agtgtcgcag ggtttgctca attaaataat acaaagggtg caccggtagt
      361 gaaaacgacc ggtggaatgc tggaaggagt caattcatcc ggcatcctga cctttaaggg
      421 agttcccttt gcacagccgc ccaccgggaa tttgcggtgg aaggaaccca tgcccgccaa
      481 accctggcag ggcattcgaa aagcagatca ttttggcccg cgggcaatgc agcttccgtt
      541 gttcggggat atgaatttcc gttcggacgg ggtaagtgaa gactgcctct atctcaatat
      601 ctggacgccg gccaaatcgg aaaaggccca gcttcctgtg ctggtctatt tttatggcgg
      661 cggcctggtg gccggtgacg ggtccgaata ccggtatgac ggggagagca tggcccgcaa
      721 aggacttgtt gccattacgg tcaattaccg gcttaccgta ttcggatttt tttcacaccc
      781 ggaactgacc aaagaatctc cgcaccatgc ctcgggcaat tatggatttc tcgaccaggt
      841 ggcggcattg aattgggtga agcagaatat cagtgccttt ggcggggatc cggacaaaat
      901 aacaatcgcc ggcgaatcgg ccggttcatt ttccgtaagc gcccttatgg cttccccgct
      961 gtcgagaaat ttaatagccg gtgcaatcgg ggagagcggc tcgctgcttg gattacaaca
     1021 cccggcaacg cttgaggaag cggaaaagcg gggcgtagag tttgcagaca gtcagcacgt
     1081 aaattcgctg gcagccttgc gggcgcttcc cgccgaacag attttgaaag cctccgcaaa
     1141 attccgtcgt ggaaattatc cggtcgatgg gtatttcttc accaggtccc cgctcgatgt
     1201 atttactgca ggccaggagg ctaaagtgcc gttgcttgtg ggctggaatt cggaagaggg
     1261 aaactggcag ggtattctgg gaaaggatca acccacgaaa gagaattatg ataaagccat
     1321 ccagaaaatg tatggcggcc atgcagacga aatattaaag gtttatcacg ccgacaacga
     1381 tgcggaggtg gaatccgtag ccactgacct ggcgtccgac cggtttatcg ggttcagcac
     1441 ctggagatgg tcggatctgc aggcaaaaac cgggggaagt aaagtatacc ggtatcatta
     1501 tgaacgccca aggccgaaaa cccgcgatgc tcaaaatacg cctgcagcca ggggggccgt
     1561 tcattcctcc gaaattgaat atgcactggg caacctgccc accaaccggg tattcgactg
     1621 gcagccggaa gattacctgg tttcggagat cctccaggct tattttgcca attttattaa
     1681 aaccggtaat ccgaacgggc tgggcttacc cgactggccc gccgttattc cggataaacc
     1741 ggcagccgta atgcgcgtgg acgtgaatac gcgtgcagaa accgaaaagc acagagatcg
     1801 ttacctggct caggaaaaat tgggaaacta agattttgac ataaacttcc ttgaatgagt
     1861 cagattaacg accggcggca acggggtatt ttgcaaacga ttgcccggag ggccatgatt
     1921 gacaaagggc ttcttcccga tttttctccg gaggccataa cccagttgaa cggcattcag
     1981 gaaaccggtg caaaagctgc cggtccggtc cgggacctgc gggatctggc ctggtgctct
     2041 atcgacaacg acgattcgct cgatctggat cagttgagcg ttgccgttcc ggcgcaggga
     2101 aaccagaccc aaatcctgat cgccattgcc gatgtggacg ccgtggtaaa aaaagattcc
     2161 gcattggaca aacacgcgag tcagaatacg acctccgttt ataccgctgc gcaaactttt
     2221 ccgatgttgc ccgagaaact ttcaacggat atcacttcgc ttaacccgga tgcagaccgg
     2281 tatgcggtgg tggtggaaat gagtatcggg gatgacgggg ctttgcaaaa ttcagacata
     2341 ttcctggcgg tcgtgcgcaa ccgcgccaag cttgcctata atagcctggc cgcctggctg
     2401 gagggaaagg ggccgatacc cgaagccgtc ggaaaggttc cgggccttga ccagaatatc
     2461 cggctccagg accgtattgc ccaaaaactt aaatcagtcc gatttgccaa tggcgcgctg
     2521 gactttgaaa ccatcgaagc ccgccccgtt tttgaaggag atgagatcag agaccttcag
     2581 gcggaaaaaa agaaccgggc gaaaaccatc atcgaagatt ttatgatcgc ggcaaacggc
     2641 attgttgcca gatggcttgc cgcccggaaa tttccttcca tgcgaaggat cgtccgcaca
     2701 cccaaacgct ggaaccggat cgttgatctg gcggcagagc ataaattcag tttgccgcaa
     2761 cagccggatt caaaagcgct ggatcagttt cttcagttgc agaaggactc ggaccccgac
     2821 cgcttccccg atctttcact gagtatcatc aagctcctgg gagccggaga atattcggtt
     2881 gaactgcccg gagaaaacgt gagcggccac ttcggactcg ccgtgaagga ttatacacat
     2941 tccacggcgc caaaccgccg g
//
LOCUS       DASYCU010000493         4875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_542777, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000493 DASYCU010000000
VERSION     DASYCU010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542777"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="VGM24_09910"
     CDS             complement(<1..1656)
                     /locus_tag="VGM24_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY4155736.1"
                     /translation="MLNSFSRIALFICVFSSSCKLMDTNRSIPGNKHLARVLNDYYED
                     RARLIPLEATQNGDNRYNDKLPIDFTDGYLDSLRNFYSGFRNKINRFNRDRLNKNDQI
                     SYDIFKREMEISLEGVNLHFSINNVTMPNIQYMPFNQFEGIPLYLGQMGSGTGIQPFK
                     TVSDYDNWIRRAGTFGAWADSAIVYFRKGMANGYVLPRCLVLKMIPEMQAMVTSDASK
                     SLFYGPANLIPADFSATDKARVTKDLLALIRETISPAYQRLGDFLQKEYLPKSRSTIG
                     FDALPNGNKLYNYLVRYWTTTNKTPEEIYQTGLSEVARIRQEMEQTKTKAGYSGDLPS
                     FFAFMKTDPRFMPFKTPEQVLDAFRKIKKTIDPNLKKLFGHTPKMGFEIRQTEAFRAA
                     SASAEYNPGSPDGSRPGIFYVPILDATKFNITSGMESLFLHEAIPGHHYQIALQMEDT
                     LLPKFRRFAWYGAYGEGWALYCESLGKELGLYTDPYQYMGALGDEIHRAIRLVVDVAM
                     HQKKMTREEAIKYMMDNEAINEQGATAEIERYIANPAQALSYKT"
     gene            1965..2840
                     /locus_tag="VGM24_09915"
     CDS             1965..2840
                     /locus_tag="VGM24_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEY4155737.1"
                     /translation="MKFRQILLLAFSSLLLFSCVSTKKFKAAQAAAQARFDSLSAEYA
                     KLQGDLKNCNDQTADLTKQKGDLQGKVDDLNKQVAFLKDNNTQALKQLEDLSVISSSQ
                     AESINKSLENIGAKDAYIRDLQSAMARKDSLNMALVMNLRGAVADVSDSDVNIKIDKG
                     VVFIDISDKLLFKTGSSVVSSGAKTVLGKVAGVLKNQPDIEFMVEGHTDNVPYKGKGM
                     DNWDLSVERAVSVVHILQRKYNIPPAKMIAAGRGEYVPVTDNNTKEGRAANRRTRIVI
                     LPLLDQFFNLLERKG"
     gene            complement(2915..4807)
                     /locus_tag="VGM24_09920"
     CDS             complement(2915..4807)
                     /locus_tag="VGM24_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY4155738.1"
                     /translation="MSDEPKVRRPTKKTKLSDEIKHECGLAFIRLRKPFSYYLQKYGT
                     ALYGLNKLYLLMEKQHNRGQDGAGVATVKLDVEPGYPFLHRLRSNNNQPISDIFSKIH
                     SEVNDLEKYQPDITRHPGLLKGHISMMGELLLGHLRYGTQGKNNVEFCHPFIKRDNLP
                     ARNLALAGNFNLVNTDELFALINIEPGEFQKQSDLAAMMEVIHHFIVQADEESPEQLN
                     VKRVLQRALPLFDGGFTVGGLLGNGDGFVFRDAHGIRPAYYYVSDDVIVAASERSAIR
                     TVFNVGENEVLELMPGNALIVKSGGKYSIDQILAPKERRACSFERIYFSRGSDEKIYR
                     ERIALGYNLSEQILTAINQDLKNSIFSFIPNTAEVAFYGLWKGLDDYLKKVMIQRILS
                     WGNEVTEEKLSQMINRKIRIEKIAIKDVKMRTFITSDTGRNEMVQHVYDITYGTVQKG
                     ADTLVVIDDSIVRGTTLRESIVRMLARLEPKKIIIVSSAPQIRYPDCYGIDMSKLKDF
                     IAFRAAIALMKERGKENCLTEIYEHCKELERLSQLHTENIVRQVYKPFTTQEISDKIA
                     QLITPPDIQTPVQVIYQTIESLHEACPTNTGDWYFTGNYPTPGGNRVVNKAFINYMEG
                     KNVRSY"
BASE COUNT         1195 a         1168 c         1154 g         1358 t
ORIGIN      
        1 cgttttatag ctcagcgcct gtgcgggatt cgcaatatat ctttcaatct ctgccgttgc
       61 gccctgctca ttgatggctt cgttatccat catgtatttg atggcttcct cccgcgtcat
      121 ctttttctga tgcatggcca catccaccac caaacggatg gcgcggtgga tctcatcgcc
      181 caaggcgccc atatactggt agggatcggt atacagtccc agttcctttc ccagcgattc
      241 acaataaagc gcccagcctt cgccgtaggc gccgtaccag gcgaaccttc tgaatttcgg
      301 aagcagggtg tcctccatct gtaaagcgat ctggtaatga tgacccggga tcgcttcatg
      361 cagaaacagc gattccatgc cggaagtgat attgaatttt gtcgcatcca ggatgggaac
      421 ataaaatata cccggacggg atccgtccgg tgatcccgga ttatattctg cgctggcgct
      481 tgccgcccgg aaggcttccg tctggcggat ttcaaaaccc attttcggcg tgtgtccgaa
      541 aagttttttc agattcgggt cgatggtctt ttttattttt ctgaatgcat ccaatacctg
      601 ctccggcgtt ttgaagggca taaaccgcgg atcggttttc ataaatgcaa aaaaagacgg
      661 aaggtctccc gaatacccgg cttttgtttt tgtctgttcc atttcctgcc tgatgcgggc
      721 gacttcggaa agtccggtct ggtagatttc ttcgggcgtt ttattggtgg ttgtccaata
      781 ccggaccagg taattgtata atttatttcc gttgggaagg gcgtcaaagc caatagtgct
      841 gcgggatttg ggcaggtatt ctttttgcag gaaatctccc agccgctgat aggccgggct
      901 aatggtttcc cggatgaggg ccagcagatc tttggttaca cgtgctttat cggttgcgct
      961 gaaatcggcc ggtataaggt ttgcaggtcc gtaaaaaagg cttttgcttg catcggaagt
     1021 gaccatggcc tgcatctccg gaatcatttt cagcaccagg cacctgggga gcacataacc
     1081 attcgccatt ccttttctga agtaaacaat ggcactgtct gcccaggcac cgaatgttcc
     1141 cgccctgcgg atccagttgt cgtaatcgga aacggtttta aaaggctgta tgcccgttcc
     1201 gctgcccatc tggcccagat acaacggaat cccctcaaac tggttaaagg gcatgtattg
     1261 aatattgggc atcgttacgt tattaatgga aaaatggagg tttactcctt caaggctgat
     1321 ctccatttcc cgtttgaata tatcgtaact gatctgatcg tttttattga gtctgtcgcg
     1381 gttaaaccgg ttgatcttat tccggaagcc gctgtaaaaa ttacggagtg agtccaggta
     1441 accatcggta aaatcgattg gaagtttgtc gttataccgg ttatctccgt tttgcgtcgc
     1501 ttccagcgga atcagccgcg cccggtcttc gtaataatca tttaataccc gggccagatg
     1561 tttgtttccc ggtatggacc ggttcgtatc cataagcttg caggaggagg aaaatacgca
     1621 aatgaacagc gcaatccttg aaaaggagtt caacatgaaa tcgggtttaa taagtaaagc
     1681 aaaggtattt caatacttcc cgcagaaggg cagtttaaaa atttcaacga ccgaatcgct
     1741 tattttttct tttttgatat atcttcgcga aaattccggg attatcctac tccctgttca
     1801 atacccacct taccttatgt ttttgggcta acttctgttt aaaaatacaa tattattcaa
     1861 gaaaaccggg tctgggactg gattggtaca acaattgcta ttgcgattat atatttttaa
     1921 ggacgcacag atttattcat taacacttat taaaaatgat tcttatgaag tttaggcaaa
     1981 ttttactttt agcgttttca tccttgctgc tcttttcctg tgtgagcacc aagaaattca
     2041 aggctgccca ggctgctgcc caggcccgct ttgattcatt gagcgctgaa tatgcaaaac
     2101 ttcagggaga cctgaagaat tgtaatgacc aaacagcaga tctgacgaag caaaaaggtg
     2161 atcttcaggg taaagtggat gatttaaaca agcaggttgc tttcctgaag gataataata
     2221 cacaagcgct gaaacaatta gaagatcttt ctgtcatctc cagttcacag gccgaaagca
     2281 ttaacaagtc tcttgaaaat attggggcaa aagatgccta tatccgggac ttgcaatccg
     2341 caatggccag aaaagattcg ctcaacatgg ccctcgtgat gaacctgagg ggtgctgtag
     2401 ccgatgtttc tgactcggat gtgaatatta agatcgacaa gggtgtagtc ttcatcgaca
     2461 tctctgataa attactattc aaaaccggaa gttcagtggt ttccagcggt gcaaagaccg
     2521 tgttgggtaa agtagctggc gttctgaaga accagcccga tatcgagttt atggtggaag
     2581 gccataccga taacgttccc tataaaggaa aaggaatgga caattgggac ctgagcgttg
     2641 agcgcgcggt ttccgtggta catatcctgc agcgtaaata caatattccg ccggctaaga
     2701 tgattgctgc cggacgcgga gaatatgttc cggttaccga caacaatacg aaagaaggcc
     2761 gtgccgctaa tcgccgcacc cggatcgtga tcctaccgct gctggatcag ttcttcaatc
     2821 tgctcgaaag aaaaggataa agcttcatac gaatcaattt taaaggccct gcttacaggg
     2881 ccttttttta tttcattcga atggagccgt gctcctagta gctacgaaca tttttccctt
     2941 ccatataatt gataaaggct ttatttacca cccggttgcc gcccggcgtc ggatagttac
     3001 cggtgaaata ccagtcgccg gtattggtcg ggcaggcttc atgaagggat tcgatggtct
     3061 ggtagatgac ctgtaccggc gtttgaatat ccggcggagt gatcagctgg gcaattttgt
     3121 cggagatctc ctgtgtggta aatggcttat acacctgccg cacgatattt tcggtgtgca
     3181 gctggctgag tctttcgagc tctttgcaat gctcatatat ttcagtaaga caattttctt
     3241 ttcctctttc tttcattaat gcaatagccg cccggaaggc aatgaaatcc ttcagcttgc
     3301 tcatatcgat cccgtaacag tccggatagc ggatctgcgg ggcggaggat acaatgataa
     3361 tctttttggg ttccagtcgg gccagcatgc gcacaatact ttcccggagc gtggtgcccc
     3421 gcacaatgga atcgtctatc accacgagcg tgtccgctcc cttctgcacc gtgccatacg
     3481 taatgtcgta cacgtgctga accatttcat tgcggccggt atcggacgtg ataaaggtgc
     3541 gcatcttaac atccttgatc gcgattttct caatccggat cttccggttg atcatttggg
     3601 aaagcttctc ttccgtgacc tcatttcccc aggaaaggat gcgctggatc atcaccttct
     3661 ttaaataatc gtccaatcct ttccacaggc cgtagaaggc cacttccgcc gtattgggaa
     3721 taaaagaaaa aatactgttc ttcaggtcct ggttgatcgc cgtgaggatc tgctcactca
     3781 ggttataacc gagcgcgatc cgctcgcggt aaatcttttc atcactgccc cgggaaaaat
     3841 aaatgcgctc aaagctgcac gcccttcgtt cttttggcgc caggatctga tcgatggaat
     3901 atttcccgcc ggatttcacg atcagtgcgt ttcccggcat gagttcgagc acttcatttt
     3961 cacccacatt gaaaacagtc cggatggccg aacgttcgga tgcggcaacg atcacgtcat
     4021 cgctcacata gtagtaagcc gggcggatcc cgtgcgcatc acgaaacaca aaaccgtccc
     4081 cgtttccgag caggccgcct acggtaaagc ccccatcgaa caagggaagc gcccgttgaa
     4141 gcacccgttt cacattgagc tgctccggcg attcttcatc cgcctgcacg atgaaatgat
     4201 gaatcacttc catcatcgcg gccagatcac tttgtttctg aaattctccc ggttcaatat
     4261 tgatcaatgc aaacaattca tccgtattca ccaggttgaa attgccggcg agggcaagat
     4321 tgcgtgcggg aagattatct ctttttataa acggatgaca aaattcaaca ttattctttc
     4381 cctgtgttcc ataccgaaga tggcccagga gcagttctcc catcatggag atatggccct
     4441 ttagcagacc gggatgccgg gtaatatccg gctgatattt ttccaggtca ttcacctctg
     4501 aatgaatttt tgaaaagata tcggagatcg gctggttatt gttgcttctt aaccggtgca
     4561 ggaaaggata acccggttcc acgtccagtt ttacggtggc cacgcccgcg ccatcctgac
     4621 cgcggttatg ctgcttttcc atgagcaggt aaagcttgtt taagccatac aatgccgtac
     4681 catatttttg gagataataa gagaaaggtt tgcgaagccg gatgaatgcc aatccgcatt
     4741 catgtttaat ttcgtcgctc aatttcgtct ttttagtggg gcggcggacc ttcggttcgt
     4801 cgctcaaatt tgtcttttac ggccgttgaa tctcccgact cacgggcata aagaaaagga
     4861 agcgcaaagt taact
//
LOCUS       DASYCU010000494         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1557970, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000494 DASYCU010000000
VERSION     DASYCU010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557970"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VGM24_09925"
     CDS             <1..1296
                     /locus_tag="VGM24_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY4155739.1"
                     /translation="DKMILGGIYDQLGGGFARYSTDGEWLAPHFEKMLYDNALLISAI
                     SEAYQITGKPLYRKAIEETMGFITREFLSPEGGFYAALDADSEGEEGRYYVWDKVETD
                     RLLGEQAAAFSAVYDISSGGNWEGKNILRIRDPARMLPEETDRACRQLLLKVREKRVR
                     PQLDDKILLGWNALMISACCRAFAALGKTEYRELAVRAMQFIEEKFRGKGLYHFHHTY
                     KKGKATVPAFLDDYAYLIDACIQLQEITGNGTYLQKAEELSQLVVRHFSEPETGLFYY
                     THEEQDDAVLRKKEIYDGATASGNAVMAGNLLYLGIALDHGEWRTRSAGMTKGMEEVL
                     LKYPGSFGVWATLINAFAYGMYEIVLGGDEQQEKQTAFLARFVPNRIFQVTSADQPDF
                     PLLRNKPVSGLSQFFLCRNYACQQPVTEPDELFQLMETP"
     gene            1463..>2247
                     /locus_tag="VGM24_09930"
     CDS             1463..>2247
                     /locus_tag="VGM24_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY4155740.1"
                     /translation="MKNLSVLMSLSAVVALTGCGKIGKSHKGLPDDGQLHGVAPASRY
                     NMTKPPGMVYIPPGTFHMGPSDEDINYAYTARNKQVSIQGFWMDATEITNNEYRQFVN
                     WVRDSTAATMMQYGKEVDGKFQVDWKKASTIKWGDKATIEKIDQLILTPENRIYGRKE
                     IDPSKLSYHSETFNLKEAASKANEGKPRSQFIEKKDIKIYPDTLVWIRDFSYSYNEPM
                     TKRYFSHPAFGNYPVVGVNWNQATAFCEWRTLILNSYLDRTKRA"
BASE COUNT          621 a          526 c          577 g          523 t
ORIGIN      
        1 gacaaaatga tcctgggagg aatatatgat caattgggag gaggatttgc gcgttattcc
       61 accgatgggg aatggctggc gccacatttt gaaaaaatgt tatatgacaa tgccctgttg
      121 atttctgcca tcagcgaagc ctatcagatt accgggaaac cgctttaccg caaggccatt
      181 gaggaaacca tgggttttat tacccgggaa ttcctttcac ccgaaggggg attttacgca
      241 gccctggacg cggatagcga aggagaagaa ggccgctatt atgtgtggga caaggtggaa
      301 acggatcggc tgctgggtga acaggcggct gctttttcgg ctgtttacga tatcagcagc
      361 ggcggcaatt gggagggtaa aaatatcctg aggattcgcg atccggcccg gatgttacct
      421 gaggagacgg accgggcctg ccggcaattg ttgctgaaag tcagggagaa aagggtcagg
      481 cctcagctgg atgacaagat cctgctggga tggaatgccc tgatgatttc cgcctgctgc
      541 agggcctttg ccgcactggg gaaaacagaa taccgggaac tggcggtccg ggctatgcaa
      601 tttatcgagg aaaagttcag gggaaaaggg ctttaccatt ttcatcacac ttacaaaaag
      661 gggaaggcga ccgttccggc ttttctggac gactatgcct atctgatcga cgcctgtatt
      721 cagttgcagg aaattaccgg gaacggaaca tatcttcaaa aggctgaaga actcagccaa
      781 ctggtcgtcc gccatttcag cgagcctgaa accggactat tctattatac gcatgaggaa
      841 caggacgacg cggtccttcg aaaaaaagag atctatgatg gggcaacagc ctccggaaat
      901 gcagtcatgg caggcaattt gctttatctc ggcatcgcac tcgatcatgg ggagtggaga
      961 acccggagcg cagggatgac caagggcatg gaagaggtgc tgctgaaata tccgggatct
     1021 ttcggcgttt gggcaacttt gataaatgcc tttgcgtatg gaatgtatga gatcgtactc
     1081 ggaggagatg agcaacagga aaaacagacc gcatttttgg cccgattcgt acccaacagg
     1141 atatttcagg taacttcagc agatcagccg gatttccctc ttttgagaaa taaaccggtc
     1201 tcgggtttgt cccaattttt tctttgccgg aattacgcct gtcaacagcc tgtaacagaa
     1261 ccggatgagc ttttccagct aatggaaacc ccttaaaaaa aacctgtcaa aacagcctga
     1321 aactacaata agccgcgccc tttcaccgtt aataatccac aggcaatact ttgacttgtt
     1381 aaaaattaat tttatacttg cgatgaagat attctcaaaa gagatagatt atttttgtcc
     1441 aatacccttt aatattatga aaatgaaaaa cctatccgtt ctgatgtcac tctctgcggt
     1501 tgtggccctg acaggttgtg gcaaaattgg aaaatcccac aagggattac cggatgatgg
     1561 tcagttgcat ggtgttgccc ctgcttcgag gtataacatg acaaagcccc ccggcatggt
     1621 ttacatacca cccggaacct tccacatggg gccaagcgac gaagatatca actacgctta
     1681 tactgctcgc aataaacagg tgtctatcca gggattctgg atggatgcca ctgaaattac
     1741 caacaacgag taccgccagt tcgtgaactg ggtccgggat tccaccgctg ccacgatgat
     1801 gcagtacggt aaggaagtgg acggcaaatt ccaggttgac tggaaaaaag ccagtaccat
     1861 caaatggggt gacaaggcaa cgatcgaaaa gatcgatcag ctgattctga cccctgaaaa
     1921 ccgcatttac ggccggaagg aaatcgatcc ttccaagctt tcctaccatt cagaaacttt
     1981 taacctgaaa gaagcggcat ccaaggccaa cgaagggaag ccgcgttccc agttcatcga
     2041 aaagaaagac atcaagattt accctgatac cctcgtctgg atccgggact tctcgtattc
     2101 ttacaacgag ccgatgacca aaagatattt ttcccatccc gcctttggca actatccggt
     2161 ggtgggtgtg aactggaacc aggccacggc cttctgcgaa tggcggaccc tgatcctgaa
     2221 ttcttacctc gatcgtacca agagggc
//
LOCUS       DASYCU010000495         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1993395, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000495 DASYCU010000000
VERSION     DASYCU010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993395"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VGM24_09935"
     CDS             <1..633
                     /locus_tag="VGM24_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4155741.1"
                     /translation="GLTQFFLWIGLIVVLATVAQAFIPHELLSEVNHLQQANAMANAS
                     AIQTSEAARQIFELKQSVANANWMLIIGCFVFYFLGGYLFYASLFAAVGSVVNEDPQE
                     AQSLLLPISMPIIFSFIIMSIAAQDPGGSLSVWASLIPFSSPIVMMARIPYGVPTGVP
                     YWQLLLSMALLAAGFLFTTWMAGKIYRTGILMYGKKPSWKTMWKWAWRKQ"
     gene            complement(637..1413)
                     /locus_tag="VGM24_09940"
     CDS             complement(637..1413)
                     /locus_tag="VGM24_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155742.1"
                     /translation="MNKPLLVLFGAGCLISCHKDGKGPVNSWTWFGTQNPAVYNYVLI
                     NANQVINKADSGELHIGVSAAFIDSNNNQITVVNALTVNDQEIQPGVDYTYNYNYSSG
                     TPKGAPVFGSNVMVTIRGTGADDTISRSVYLPKQLVQLISGYPDAIQRSKGLQLTWLP
                     DEENAWGNVLIQLFYYKDLSRKADSTLPEKINTVSITTPDNGNYFLTSKDLESFPVRA
                     VMGITIARGSQNVAILPLSRKRIYYFSSASVSTTPLPVIQ"
     gene            1504..2466
                     /locus_tag="VGM24_09945"
     CDS             1504..2466
                     /locus_tag="VGM24_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY4155743.1"
                     /translation="MEFSNLITRLFGIPYPIIQAGMVWNSGWRLASAVSNAGGLGLIG
                     SGSMYPDVLREHIRKCRAATSAPFGVNIPLLYPDTEQHIRIVREEKIPIVFTSAGNPE
                     TWTRNLKQDGIRVVHVVGSSRFASKAEAAGCDAVVAEGFEAGGHNGREETTSLVLVPA
                     VVDAVKIPVIAAGGISNGRQMFAVMSLGAQGIQMGTRFVMSEESSSHPDFKNKVLHAT
                     EGDTALSLKSLMPVRLIRNRFYEQVTAAEQQGASVSRMKELLGRGRAKRGMFEGDLEE
                     GELEIGQASALISDILPARTIIENIWAEFRKTRANPLSCDSEAS"
     gene            2490..>2711
                     /locus_tag="VGM24_09950"
     CDS             2490..>2711
                     /locus_tag="VGM24_09950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4155744.1"
                     /translation="MIPITFCCCNLYASNGKTGPCLIRGQVVDAITKKPVSGVVVSAV
                     ITGNNNSNAVITDNEGYYYFKELPSNHVNL"
BASE COUNT          644 a          702 c          713 g          652 t
ORIGIN      
        1 ggccttaccc agttcttctt atggatcggg ctgatcgtcg ttctggcaac ggttgcccag
       61 gcgttcattc cgcatgaact gctcagcgaa gtgaatcatc tgcagcaggc caatgcgatg
      121 gccaatgctt cggccattca aaccagtgaa gccgcgcggc agatctttga attaaagcaa
      181 tcggtggcca atgcaaactg gatgctcatc atcggctgtt ttgtgtttta tttcctgggc
      241 ggttatcttt tttacgcctc cctgtttgct gccgtgggaa gcgtggtcaa tgaagatccg
      301 caggaagcgc aatccctgct gctacccatc agcatgccga tcattttttc ttttatcatt
      361 atgtccatcg ccgcgcaaga ccccggcgga tccctttcgg tctgggccag cctgatcccc
      421 ttcagttccc cgatcgtgat gatggcccgg atcccgtatg gcgttcccac cggcgtgccc
      481 tactggcaat tattgctgtc catggccctg ctggccgcgg gatttctttt caccacctgg
      541 atggccggaa agatttacag aacgggaatc ctgatgtacg gcaaaaagcc cagctggaag
      601 accatgtgga aatgggcctg gagaaaacaa taaaaattac tggatgaccg gcagcggggt
      661 cgtactcacc gaggcgctgg aaaaataata gatccgcttg cgggataaag gcaggatggc
      721 cacattctgt gatccccggg caatcgtgat ccccataacg gccctgaccg gaaatgattc
      781 caggtctttt gaagtcagaa aataatttcc gttatccggc gtggtgatac tgaccgtatt
      841 gatcttttcc ggaagggtcg aatccgcttt tctgctgagg tctttataat aaaacagctg
      901 gatcaacacg tttccccagg cattttcttc atccggcaac caggtaagct gaagcccctt
      961 gcttcgttgt atggcatcag ggtaaccgga gatcagctgc acgagctgtt tgggcaggta
     1021 aaccgatctg ctgatcgtat cgtcggcgcc ggttccgcga atggtcacca tcacgttcga
     1081 tccgaaaacg ggcgcgccct tgggcgttcc ggaagaataa ttgtaattgt aggtgtagtc
     1141 gaccccgggc tggatctcct ggtcattcac cgttaacgca ttcaccacgg tgatctggtt
     1201 gttgttgctg tcaatgaagg cagcgctgac cccgatgtgc agttcaccgc tgtcggcttt
     1261 attgatcacc tggttggcat tgatgaggac gtaattgtat accgccggat tttgagttcc
     1321 gaaccaggtc cagctgttga ccggtccttt tccgtctttg tggcaggaaa tcaaacagcc
     1381 cgcaccaaac agtaccagca aaggtttatt catgccttga acttaaagaa tgctccataa
     1441 atatactttt ttggcaagaa ataaaaaaaa caaactatca acgggctgta cctttgaaac
     1501 tgaatggaat tttccaatct catcacccgg ttgttcggga tcccttatcc cattatccag
     1561 gccggcatgg tctggaacag tggctggcgg ctggcttcgg cagtaagcaa tgccggcggt
     1621 ctcggcctca tcggaagcgg aagcatgtac ccggacgttc ttcgtgaaca tatccgcaaa
     1681 tgcagggccg cgacctccgc cccctttggg gtgaacatcc ccttacttta cccggatacc
     1741 gaacaacata tccgcatcgt ccgggaagaa aaaattccca tagtctttac atcggccggt
     1801 aacccggaga cctggacccg taacttaaaa caggatggaa tccgggtcgt gcacgtggtc
     1861 ggcagcagcc ggtttgccag caaggcggaa gcggcgggct gcgacgcggt ggttgccgag
     1921 ggctttgagg ccggcggaca taacgggcgt gaggaaacca cttcccttgt actggttccg
     1981 gcggtggtgg atgccgtaaa aatcccggtc attgcagcgg gaggaatcag caacggccgc
     2041 cagatgtttg cggtcatgag cctgggcgcc cagggcatac agatgggaac ccgttttgtg
     2101 atgagcgagg aatcttcttc ccatccggat tttaaaaata aagtactcca tgcaacagaa
     2161 ggagacacgg ccttgtcttt aaagtcactg atgcccgtcc ggctgatcag gaaccggttt
     2221 tatgaacagg tgacggcagc cgaacaacag ggcgcttctg tttcccggat gaaagaattg
     2281 ctgggaagag gaagggcaaa aagaggaatg ttcgaaggcg atctggaaga aggagaactg
     2341 gaaatcggac aggccagcgc cctgatcagc gacatacttc ccgcccggac catcatagaa
     2401 aatatatggg cggaatttag gaaaacccgc gccaatccgc tatcttgtga ctccgaagca
     2461 tcatgaaaaa atatatttat atactttttt tgatccccat tactttctgc tgttgcaacc
     2521 tgtatgcaag caatgggaaa accgggccct gtcttatccg cggccaggtg gttgacgcca
     2581 tcaccaagaa gccggtctcc ggcgtggttg tttccgccgt cattaccggg aacaacaatt
     2641 ccaacgcagt cattacggat aacgaaggat actattattt taaagaactt ccctccaatc
     2701 atgtgaacct t
//
LOCUS       DASYCU010000496         3817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_981536, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000496 DASYCU010000000
VERSION     DASYCU010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981536"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VGM24_09955"
     CDS             complement(<1..329)
                     /locus_tag="VGM24_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEY4155745.1"
                     /translation="MKKALSAIAFISFLYLNSFAQQIKIPSPSPVQTVKQEFGLSSIE
                     LSYSRPGVKGRKIFGDLVPYGKVWRTGANQATTLTFGDSVIIGDTPIPPGRYGLLTVP
                     GPDEWTI"
     gene            complement(422..>3817)
                     /locus_tag="VGM24_09960"
     CDS             complement(422..>3817)
                     /locus_tag="VGM24_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155746.1"
                     /translation="NGKDRLSSGDSIYFIFRGSTGWSIFFLQNQAQKIKDKDTGVQAH
                     EQSLANFTSPCLASGKEIFNSFLLVHYCQQAGPDSGGKIIYQDFQQGMEQDIDLDSLI
                     PRHQGVRSPGMTDISLEGADYKLFTYPFQLGRHRLVLCGLIKTDSYNAQLHVIPVSTL
                     YTLVICLVLFLLSLPFLKIFMMNERDRIYAFNLVIGIVFLFAMASLITIISTQLIMLR
                     QGRIQVNANLDSLSVKIEDSLSAELTDAQNELLYFDQRLDNYMDSSKTDTLSITGTPS
                     FLITTDSSYSNPVFNKNQRVLSGHFPLYHNSDHISWISESGQGRVRVRYIPEKTAEFL
                     LSTKKKVDTISFVDVRARSYYQELHQRFRYKKANDSTLLLAPVQSWASGEFRVNICRY
                     SSIKGFMMELMETRLYSLVNTVLPAGYDYCLFNDSGNVLIHSDTLKSLRENFFDETGR
                     IPWILGAVKGRQGIQSGSVNFYGTNYTLRIQPLKQHPLFLAVFYNNNYLGPANLRVLT
                     FSLFFCLLTYGLLVLLFLILYTRDPKTCLYSPVDYYRRLIPRKEKLTLYFNGCWFLIL
                     YMAVFSIAAVFSPVVDYDTDYTVLVFGLLTPFNLIYALWLFQNKAGKLNLKESFRYIR
                     PAVLTGLFIFALSYISGWKLEPVSFFTLEILLWLGFEGIVLRKGKRWQTVTTRLRHKI
                     RDISGWKKKQEEEKKTYSFSWFATLFIFAIAVVPPLEFSWFAYNHELRQTIKKEQLEL
                     ADGIQKRERTVRAFLHSNQPDFSGNDLNYNNVQYHEGIYPVYPGKIISLPGSSRFTRN
                     EASHSSRSEHFYLSIASTINLFYDDPAGLPPLYDSSRHDNLWHWDLNKDYYVFDYSRA
                     KWKIPPALSDASPPDISFKIISHIPGGLRIDGRLQLVFAGMILALLLAIYWITKNIAR
                     QVCLTKIVGNAGPGLDDRFGKSGTGPWEKCLAENNMGLSAAELLAKLKGEYAAFDPKG
                     KDLVTQESDIVRRSAEMQPLFECVWKTLDDKEKYLLYCLANDGLLNHKNEPVIYGLLH
                     KNLLLIYDQRIRLISYSFRDFILSRRNTAQENALLAKMQSGASWTFMRTIVLVVVMSV
                     FIFLFLTQQEVSAKIIALVTSLSALLPFLLKFGSSTAAADSKK"
BASE COUNT          948 a          897 c         1026 g          946 t
ORIGIN      
        1 atgatggtcc attcgtcggg tccgggaacg gtgagtaagc catacctgcc gggagggatg
       61 ggcgtgtcgc cgatgatcac cgaatctccg aaggtcagtg tggtagcctg gttggcgcct
      121 gtccgccaca cctttccgta aggcaccaga tcgccgaaga tcttcctgcc ctttacccct
      181 ggtcttgaat aggagagttc gatggaagaa agaccaaatt cctgttttac ggtctggaca
      241 ggtgaaggtg atggtatttt tatctgctgg gcaaagctgt tcaggtatag gaaagaaatg
      301 aatgcaattg cggataatgc ctttttcatg agactatatt atggttagtg ttttgcaaag
      361 gtatacggtt gccggatcac tatttccgga ttttattttc ccgggcggat cttcatcccg
      421 cctatttttt gctgtctgcc gctgccgtcg aagaaccgaa ttttaaaagg aagggcagga
      481 gggcggaaag gctggtgacg agtgcgatga tcttggccga tacttcctgc tgggttaaaa
      541 ataagaatat gaaaaccgac atgaccacca ccaggacgat ggtccgcatg aatgtccatg
      601 atgcgcccga ttgcattttg gccagcagcg cgttttcctg ggcggtattc ctcctggaga
      661 gaatgaaatc ccggaaactg taactgatca gccggatgcg ctgatcataa atcaggagca
      721 ggttcttatg gagcaggccg tagatgaccg gttcgttctt gtggttcagc aggccgtcat
      781 ttgccaggca gtacagcagg tatttttctt tatcgtccag tgttttccaa acacattcaa
      841 aaaggggttg catttctgcc gatctgcgaa caatatccga ttcctgggtg acgaggtcct
      901 ttcccttcgg atcaaatgcg gcgtattctc ctttcagttt agccagcaat tccgcagcag
      961 ataaacccat attattttca gccaggcatt tctcccaggg gcccgtcccg ctttttccaa
     1021 accggtcatc caggcccggt cccgcgttcc ctacaatctt ggtaaggcag acctgccgcg
     1081 cgatgttttt ggtgatccag taaatggcca gcaggagcgc cagtatcatg cccgcaaaaa
     1141 cgagttgaag acggccgtcg atccggagcc cgccgggaat atggctgatg attttgaagc
     1201 tgatgtccgg agggctggca tcgctcagcg cgggaggtat tttccatttt gcccgggaat
     1261 aatcaaacac ataataatct ttattgaggt cccagtgcca gaggttgtcg tgccgcgagc
     1321 tgtcatacag cggcggcagg cccgccggat catcataaaa taaattgatg gtgctggcga
     1381 tcgaaagata aaaatgttcc gaacggctgc tatgactcgc ttcgttccgg gtgaaccggg
     1441 aactcccggg caaagaaatg atctttcccg ggtaaaccgg atatatgccc tcgtgatatt
     1501 gaacgttgtt ataattcagg tcattcccgg aaaagtcggg ctggttgctg tgaaggaatg
     1561 ccctcacggt tctttctctt ttctgaatcc cgtcggccag ttcgagttgt tcttttttaa
     1621 tcgtctgccg gagttcatgg ttataggcaa accagctgaa ttcgagcgga ggaaccacgg
     1681 cgatcgcaaa gatgaaaaga gtggcaaacc agctgaagga ataggtcttt ttttcctcct
     1741 cctgcttttt tttccacccg gaaatatccc ggatcttgtg ccggagacgg gtcgtaacgg
     1801 tctgccagcg ctttcctttc cgcagcacga tcccttcaaa gcccagccag agcaggattt
     1861 ccagcgtgaa aaaggaaacg ggttcaagct tccatccgga aatatagctt aaggcaaaga
     1921 tgaaaaggcc ggtgaggacg gccgggcgga tatatctgaa cgattccttc aggtttagtt
     1981 tgcctgcctt gttctgaaaa agccagaggg catagatcag gttgaatggg gtcaggaggc
     2041 caaataccag cacggtataa tccgtatcat aatcgacaac gggactgaag acggctgcaa
     2101 tggaaaagac cgccatataa aggatcagga accagcaccc gttgaaataa agggtgagtt
     2161 tttctttgcg cgggatcagg cggcggtaat aatccaccgg agaatacagg caggttttgg
     2221 ggtcccgggt atacaggatc agaaagagca aaaccagtaa tccgtatgtc agcaaacaga
     2281 aaaataagga aaacgtgagg actcttaaat tcgccggtcc gagataatta ttattataaa
     2341 aaacggccag aaagagcgga tgctgcttta agggctgtat ccggagcgta taatttgttc
     2401 cataaaaatt tacgctgccc gactggattc cctggcggcc tttaacggct ccgaggatcc
     2461 agggaatacg ccctgtttca tcaaaaaagt tctcgcgcag gcttttcagg gtgtcggaat
     2521 gaatcaggac attccccgaa tcattaaaca ggcaatagtc gtaacccgcc ggcaacaccg
     2581 tattgaccag cgaatagagc cgggtttcca tcagttccat cataaaccct ttgatcgagc
     2641 tgtagcggca gatgttcacc cggaattccc ccgaggccca gctttgtacc ggcgccagca
     2701 agagggtgga atcgtttgct tttttgtacc ggaagcgctg gtgcaattcc tggtaatacg
     2761 acctggcccg gacatccaca aaagaaatgg tatccacctt tttctttgta ctcagcagga
     2821 attcagctgt tttttccggg atatatctaa ccctgacgcg gccctgtccc gattcgctga
     2881 tccaggaaat atggtccgaa ttatggtaga gggggaaatg cccggatagt accctctggt
     2941 tcttattaaa aacggggttg gaatagctgc tgtcggtcgt gatcagaaaa gacggggtgc
     3001 ccgttatact cagggtatcg gtcttcgagg agtccatata gttatccagc cgttgatcga
     3061 aatagaggag ctcattctgg gcatcggtca attctgcgga gagggaatcc tcgatcttta
     3121 cagagagtga atcgaggttg gcattcacct ggatccttcc ctggcggagc atgatcagct
     3181 gtgtgctgat gatcgtgatc aggctggcca tggcaaaaag aaagacgatg ccgatgacca
     3241 ggttaaaagc atagatccgg tcgcgctcat tcatcatgaa tattttcagg aagggaaggc
     3301 tgagcaaaaa aagaacgagg cagatgacaa gggtatacaa tgtacttacc gggatcacgt
     3361 gcaactgcgc attgtagctg tctgttttta ttaggccgca aagcacgagc cggtgccggc
     3421 ctagctgaaa gggataggtg aacagtttat aatccgcgcc ttccagggag atatccgtca
     3481 tgccaggcga acgcacgccc tggtgcctcg gaatcaggct gtccaggtcg atatcctgtt
     3541 ccatgccctg ctggaaatcc tggtatatga tcttgccgcc gctgtcgggc cccgcctgct
     3601 ggcaataatg gaccagcagg aaggagttga atatttcttt tcccgaagca aggcagggcg
     3661 aggtaaaatt ggccaggctc tgttcatgcg cctgcactcc ggtgtcttta tcttttattt
     3721 tttgggcctg gttctgtaga aagaaaatgc tccagccggt tgagccgcgg aaaataaaat
     3781 aaatcgagtc tccggaagag aggcggtctt taccatt
//
LOCUS       DASYCU010000497         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1417035, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000497 DASYCU010000000
VERSION     DASYCU010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417035"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..661)
                     /locus_tag="VGM24_09965"
     CDS             complement(95..661)
                     /locus_tag="VGM24_09965"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY4155747.1"
                     /translation="MKLLFSGFCLAAGLFALQACNNNPSSTSTEDAVDSAKSVNKAVQ
                     PVQKDASDFAVAAANGGMMEVELGKIAEEKAMNQRVKDFGSMMVKDHTAANDNLKSIA
                     STLNITLPDSVSDDTRKEIDKLKMKKGKDFDKAYVDMMLDDHKKDIAEFRKCADNCSD
                     STIKSFASTTLPTLEKHLDSIQAISGKK"
     gene            862..1173
                     /locus_tag="VGM24_09970"
     CDS             862..1173
                     /locus_tag="VGM24_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEY4155748.1"
                     /translation="MATQLNNPGTREESLADIEILTDPEEPYSLIVWNDEVNSFDWVI
                     QTLVEVCKHSTEQAEQCAMIIHTQGKYAVKRGSYEELKPMCDAITERGIGATIEVLSP
                     S"
     gene            1216..1869
                     /gene="aat"
                     /locus_tag="VGM24_09975"
     CDS             1216..1869
                     /gene="aat"
                     /locus_tag="VGM24_09975"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY4155749.1"
                     /translation="MPLYVLDKELEFPPAELADEDGLLAIGGDLSIERLLLAYQKGIF
                     PWYENNYILWWCPDPRFVLFPSELKVSNSMKQVLKKGELEFSIDRNFNDVISACKTVS
                     RRGQEGTWITEDMRNAYLNLHMQGYAHSAEAWLNGDLVGGLYGVRIGNIFFGESMFSK
                     LTNASKFAFIRYVRQLQSEGVELIDCQVYTAHLESLGARMIPRPDFLARVQSLSTRL"
     gene            complement(1864..3243)
                     /locus_tag="VGM24_09980"
     CDS             complement(1864..3243)
                     /locus_tag="VGM24_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY4155750.1"
                     /translation="MYAIVDIETTGSYAANNGITELAIVLHDGNKTVDRFETLVNPQM
                     PIPRYIQALTGIHNGMTDLAPLFNEIAGRVYEMLKDAVFIAHNVNFDYSFLKFHLEAS
                     GYSFNSKKLCTVRLSRKVFPGLPGYSLGKLCRELAIPMENRHRAGGDADATVKLFEKI
                     LLGDSQGHLRQMLQGRSGEQYLPPNLPVEQINSLPYSSGVYYFHDQKGKVIYVGKAKN
                     LRYRVCSHFSNNHPGLRKQEFLRNIYSLSWQECGTELMAFLFECVEIKRLWPKYNYSL
                     KRFEQSYGLYAFEDLNGYLRLTIEKKKQQLHSFYCFNGIWEGKQIIGRLVREFGLCPK
                     LCFLQRGEDICEGIKSRYCSGACIQKEEASLYNRRVQEALNSLQQALPSFVIRDKGRN
                     SAEESCVLIENGRFYGMGYLDKEEQILDLDLLKEHLTAYPENDYMRGLVYQYAEKWPG
                     KKRPMGSGS"
BASE COUNT          859 a          837 c          761 g          913 t
ORIGIN      
        1 aaccagtgtc ttcatttgat gtaagcctta aatttctcct gcctccggcc atcttttttt
       61 ggcgtaaagc taagaagact tctttttcag tctcctactt tttccccgaa atagcctgaa
      121 tggaatccag gtgcttttcc aaagtgggga gggtggtgga agcaaaagac ttaatggtcg
      181 aatcagaaca gttatcggcg catttcctga attcggcaat atccttttta tggtcatcca
      241 gcatcatatc cacgtatgcc ttgtcgaaat cctttccctt tttcattttc agtttatcga
      301 tttccttgcg ggtatcatcg ctgacggagt cgggcaaagt aatattcagc gtactggcta
      361 tgctctttaa attatcattg gccgcggtat ggtctttcac catcatcgag ccaaaatctt
      421 tcacgcgctg attcatagct ttttcttccg cgatctttcc gagttcaact tccatcattc
      481 ccccgttggc cgccgcgacg gcaaaatcag aagcatcctt ctgaaccggt tgaacggcct
      541 tgttcacgct cttggcgctg tcaacggcat cttccgtgga ggtggaggat ggattattat
      601 tacacgcctg cagggcaaaa aggcctgctg ccaggcaaaa gccggaaaat agtaatttca
      661 tgatcttagt tttaaatgat ttgatacccc tagctattca aacaatacgc catgcctttt
      721 aagcacataa tccggcattc tcctaaaaat ctggggataa aaggcaacaa aatgcaccgg
      781 aaaataaatt gttaaaatat ttatcccatt tttgagccct ttcgctttgc ttaaaaagag
      841 gtattgttct aattttatga tatggcaacg cagttgaaca acccgggaac aagggaggag
      901 agcctggcag atatcgaaat actgacagac cccgaggagc cttacagttt aattgtatgg
      961 aacgatgaag tgaacagctt tgattgggtt attcagaccc tcgttgaagt atgcaaacac
     1021 agcaccgaac aggcagagca atgcgcgatg atcatccata cccagggcaa atatgcggta
     1081 aaacgcggaa gctatgagga actgaagccc atgtgtgacg cgatcacaga acggggcatc
     1141 ggcgcgacca tcgaggtact ctccccatcc tgagccggct ccggctacgc cctccattaa
     1201 atcaattcac ccactatgcc cctttacgtc ctggataagg aactggaatt ccctcctgct
     1261 gaactggcgg atgaagacgg cttactggct atcggagggg atctttccat agaaagattg
     1321 ctgctggctt accaaaaggg cattttcccc tggtatgaga ataattatat tctctggtgg
     1381 tgtccggatc cccgctttgt tctgtttcct tccgaactga aagtaagcaa cagcatgaaa
     1441 caggtattaa aaaagggaga gctggaattc tccatcgacc ggaattttaa cgatgtgatc
     1501 agcgcctgca aaaccgtttc gcggcggggt caggaaggta cctggattac cgaggacatg
     1561 cggaatgcct atttaaatct gcatatgcag ggatatgccc acagcgccga agcctggctg
     1621 aacggagatc tggtcggcgg tctctatggc gtccgcatcg gaaatatttt ttttggcgaa
     1681 agcatgttca gcaaactgac caatgccagc aaatttgcct ttatccgtta tgtccggcaa
     1741 ttacagtctg aaggcgtgga gctgatcgat tgccaggtat atacggcaca cctggagagc
     1801 ctgggtgccc gcatgatccc gcgacccgat tttttagccc gggttcaatc cctgagcact
     1861 cggctatgag ccgcttccca tcggtctctt ttttcccggc catttttctg catactgata
     1921 caccagcccg cgcatataat cattttcagg ataagccgtg agatgttctt tcaataaatc
     1981 cagatccagg atctgttctt ccttgtccag ataccccatt ccataaaagc ggccattttc
     2041 aataagcacg cagctttctt cagcactgtt tcttccctta tcccggataa caaaagaagg
     2101 aagtgcctgc tgaagggaat tcaaggcttc ctgaacacgg cgattataaa gggaagcttc
     2161 ctctttttga atgcaggcac cggaacaata ccggcttttg atcccttcac aaatatcctc
     2221 tccccgctga aggaagcaca gtttggggca taacccaaac tcgcggacca gccggccgat
     2281 aatttgtttc ccttcccata ttccattaaa acagtaaaag ctgtgcagtt gctgtttttt
     2341 cttttcaatc gtcagccgca aatagccgtt aaggtcctcg aatgcataga gtccataact
     2401 ctgctcaaac cgtttcagac tataattata tttcggccac agtcttttga tctccacaca
     2461 ttcaaaaaga aaggccatca gttccgttcc gcattcctgc caggaaaggg aataaatatt
     2521 ccgtaaaaat tcctgtttcc gcaggcccgg atgattattg gagaagtgac tgcagacgcg
     2581 gtaccggaga ttttttgcct tccctacgta aatcactttt cctttttgat catggaaata
     2641 atacaccccg gaactataag gcaggctgtt gatctgttcc accggcaggt tgggcggaag
     2701 atattgttct ccgctgcgcc cctgcagcat ttgccgcaga tgcccctggg aatcgccgag
     2761 gaggatcttt tcgaaaagct ttaccgtggc gtctgcatcc ccgccggccc ggtgacggtt
     2821 ttccatgggg atcgcgagct cccggcacaa tttgcccagg ctgtatcctg ggagccccgg
     2881 aaaaaccttc cggctcagtc ggacggtaca gagttttttg gaattaaagg aatagccgga
     2941 ggcttccaga tggaatttca ggaaggaata atcaaaattt acgttgtgcg caataaaaac
     3001 cgcgtccttc agcatctcat aaacacgacc ggcaatttca ttaaacaaag gggccaggtc
     3061 ggtcattccg ttatgtatgc cggtaagcgc ctgaatatag cgcggaatgg gcatttgagg
     3121 attaaccagg gtttcgaacc ggtccaccgt cttatttccg tcgtgaagca caatagccaa
     3181 ttcggtgatt ccgttattag ctgcgtaact gcctgttgtt tcaatatcta cgatagcgta
     3241 cataaccggt agcaatctcg atttgctaat aaagttagga gtaatcacgc ttcagtcacc
     3301 cattcattta ttgatttttt gatattgccg ttcgcacccc ttgaaagcgc cctgaatccg
     3361 tccgtatagt
//
LOCUS       DASYCU010000498         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3298168, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000498 DASYCU010000000
VERSION     DASYCU010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298168"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..424)
                     /locus_tag="VGM24_09985"
     CDS             complement(110..424)
                     /locus_tag="VGM24_09985"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY4155751.1"
                     /translation="MDSNLAFNALGDPTRRSIFEKLQGGPLSVVHIAEGMRVSRPAVS
                     QHLKVLKEAQLISLKTIGNRNIYQLNRKGIMAMRNYLDKFWENALANFKALAEQTEKE
                     RK"
     gene            complement(814..899)
                     /locus_tag="VGM24_09990"
     tRNA            complement(814..899)
                     /locus_tag="VGM24_09990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(863..865),aa:Leu,seq:taa)
     gene            complement(945..1020)
                     /locus_tag="VGM24_09995"
     tRNA            complement(945..1020)
                     /locus_tag="VGM24_09995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(985..987),aa:Gly,seq:gcc)
     gene            complement(1095..1307)
                     /locus_tag="VGM24_10000"
     CDS             complement(1095..1307)
                     /locus_tag="VGM24_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155752.1"
                     /translation="MDEEPIEYKDPKLKDITYNWVDSSVFLFYLETFCIIALLIGASY
                     GLYTHRYKGKPEVVVPANTLYTPEYK"
     gene            complement(1355..2380)
                     /locus_tag="VGM24_10005"
     CDS             complement(1355..2380)
                     /locus_tag="VGM24_10005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4155753.1"
                     /translation="MPAKVSVVILNFNGRKYLEQFLPSVLASRYSNLEVVVADNASTD
                     DSLLFLNEHYPQVRQILLIRNYGYAGGYNQSLKQINSDYYVLLNSDVEVEPGWIDPVI
                     ALMENDRSIGACQPKILSYKQKDHFEYAGASGGWMDWLGYPFARGRIFDICEADKGQY
                     NDVQKIFWASGAALFVRSELYHSLKGLDTYFFAHMEEIDFCWRLQLIGYGIYVCPASV
                     IYHVGGGTLPKGNERKVFLNFRNNMIMLAKNLPRRQSFWKIPLRILMDVISAWKSLFA
                     GEAVYFFAIMEAHLAFIKWLFFKQGQSIFPSRRKAALTGWYQHSVAWKHFVLKKETFD
                     EIVGDKR"
     gene            2479..3621
                     /locus_tag="VGM24_10010"
     CDS             2479..3621
                     /locus_tag="VGM24_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY4155754.1"
                     /translation="MIGFRFNWRWLLALLGIGIVCGTILYSQFLAKKIEREEQQKIEQ
                     WLAASKAILNPADLTLPNLIRNEQHSIPIIETNEKDSITSFFNLDTIEAKKDRSYLPA
                     KLKTFRSQNPPLEIKWSDSPYTANRYYYGHTALLDQVRYFPIIQLFLVAILITFIIYS
                     ISTHNRSTQNQVWAGLAKETAHQLGTPLSSLQGWIEMLKEEGTTAETLSELQKDLDRL
                     KLISDRFSKIGSIPILEKSDIVPQIQQMVEYIRRRTTSKVQFSLDAHGTSAVEADISK
                     PLFDWVIENLFKNALDAMEGKGKISVDIKNESQQVIIDVTDTGKGISPSNIRRVFNPG
                     FTTKKRGWGLGLTLSKRIMEEYHKGDLFVKHSEPGKGTTFRIVLKK"
     gene            3695..3781
                     /locus_tag="VGM24_10015"
     tRNA            3695..3781
                     /locus_tag="VGM24_10015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3729..3731,aa:Leu,seq:gag)
     gene            complement(3870..>5428)
                     /locus_tag="VGM24_10020"
     CDS             complement(3870..>5428)
                     /locus_tag="VGM24_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4155755.1"
                     /translation="EPGGNATYVYIFGGVAVFMLMVACINFINLSTAGASKRAKEVGV
                     RKVAGSGKIQLISQFLSESILITFFGLIVAFVLVKLAMPGFNNISGKHLHFDVKPTLA
                     FIGLGLLVGLVAGIYPAFYLSSFKPIAVLKGKLASDHKSFGIRSGLVVFQFFISVALI
                     IGTIVVYQQMKYIQNKDLGYSKDQLITIPNSYFLGNREQVFKQQMLQDPRIINATVSF
                     YKPAGPSNYNNAIAYPQGNDRLLVDGVEYHVDEEYIPTLGMKMVGGRAFSKDFKTDSS
                     AIILNETAAKAFGWNNASAIGKTIIRQNTDRGSNLPFRVIGVVKNFNFKSLHEAVSPL
                     FMRLDTEGGLIFKIKTTDIAGLLATMKKQWDSYATGEPFSCSFMDDLFNNTYSTEQKT
                     GKILNIFSVLIILVACLGLFGLVTYTAEQRRKEIGIRKVLGASVSQVTRTLSRDFLKL
                     VLIASLIAFPVAWWGMNKWLQSFAYRISISWWIFFAAAFGALLIALITVSFQAIKAAM
                     ANPVKSLRTE"
BASE COUNT         1517 a         1389 c         1123 g         1399 t
ORIGIN      
        1 atgctgttta attcgagcgt ttccatgatg attttgtttt aaaataagcg gatcagatac
       61 cgacgttccc ggattcaaga accatttcac tcatagcgca ttggacttac tactttctct
      121 ctttttcagt ttgttcggcc agcgctttga aattcgccag ggcgttctcc cagaatttgt
      181 ctaagtaatt tctcatagcc atgatccctt tgcggttgag ttgatagata tttctgttgc
      241 cgatcgtctt tagactgatc aattgggctt ccttaagcac tttcaggtgt tgggaaacag
      301 ccggccggct gacacgcatc ccttccgcta tatgcacgac cgataacgga ccgccttgta
      361 atttctcgaa gatggagcga cgggtcggat cacccaatgc gttaaaagcc aaattgctat
      421 ccataaaaaa gtaagtttat atttacggta agtacaaact tacaaatatt tttgaaaccg
      481 ccaaatactt ttaatcattt tatttgggaa caacaaatgg ctcccagaaa ttattgttgg
      541 gacttctggc tgaggtccgc tctaaagatc taacttcatt aatcgccatt ttggcgcagc
      601 atcagcgttt ctatttaaaa gaaggcacgt gtatacaccg attcgcattg aaaatggctg
      661 ggtattgcct ccgccaagct ttttgaagga gatggattgc cgtttcgaac cgcgcgggtc
      721 aatgaagtga tatgattgat gttcaagctg gaccctggtt tcagcggaag taaaaaataa
      781 aaaaacggga attttcgaaa atccccgctt cggtacccca gacgggaatc gaacccgtac
      841 ccgcatttct gcagacagga ttttaagtcc tgcgcgtcta cctgttccgc caccagggta
      901 tgctgtcaaa taaaaaattc catccctttt agcggatgga atcctggagc ggaagaccgg
      961 gctcgaaccg gccacctcga ccttggcaag gtcgcgctct accaaatgag ctacttccgc
     1021 atataaaacg ataataagaa ctattccggg acggcgaatt taagcattcc accgttccct
     1081 gaaaaaagtg attattattt gtactccggc gtatacagcg tattcgcggg aaccaccact
     1141 tccggcttgc ctttgtaccg gtgcgtgtac aacccgtaac tggctccgat caggagcgct
     1201 attatacaaa atgtttccag ataaaacagg aatacggatg aatccaccca gttatacgtg
     1261 atatccttga gcttggggtc tttgtattct atcggttcct cgtccataat aatcagttaa
     1321 ttacagcgca aaaataaccc attcggcgaa aaaatcatct tttatccccc acaatttcat
     1381 caaaagtttc cttcttcagc acaaaatgct tccaggccac actatgctga taccaacccg
     1441 ttaaggctgc ttttcgccgg ctgggaaaaa tactctggcc ctgcttgaaa aacagccatt
     1501 ttataaaggc cagatgcgct tccattatcg caaaaaagta caccgcttca ccggcaaaca
     1561 gggatttcca ggccgagatc acatccatca ggatccgcag cgggatcttc cagaacgact
     1621 gccgtctggg taaatttttt gccagcatga tcatattatt ccggaagttc aggaacacct
     1681 tcctttcatt tcccttgggt aaagtgcccc ctcccacatg gtatatcacg gaggcaggac
     1741 aaacatatat gccatagccg atcagctgca agcgccagca aaaatcaatc tcctccatat
     1801 gcgcgaaaaa ataggtatcc aggcctttca gggaatgata taattccgac cggacaaaca
     1861 gggccgcccc gctggcccag aaaattttct gaacatcatt atactgcccc ttatcggctt
     1921 cgcaaatatc aaatatcctt ccgcgcgcga aaggatatcc gagccagtcc atccaccccc
     1981 ccgaggcgcc cgcatattca aaatgatcct tctgtttata actcaggatc ttcggctgac
     2041 aggcaccgat cgaccggtca ttctccatca gggctatcac cggatctatc cagccgggct
     2101 ccacttccac atccgaattc agcaatacgt aataatcgct gtttatctgt ttcagcgact
     2161 ggttataccc cccggcgtaa ccataattcc ggatcagtaa gatctgtcgc acctggggat
     2221 agtgctcatt gagaaacagc agggaatcat cggtggacgc attgtcggcc accaccactt
     2281 ccaggttgga atatctgctt gccaataccg aaggtaaaaa ctgctccagg tacttgcgcc
     2341 cgttaaaatt gagaattaca actgatactt tagccggcac ttgaattcat tacaatataa
     2401 aaagtgggcc aaaaataata catctgcccg gaatgattgc tgaaatatga tttgcccggg
     2461 tttgattact ttagctccat gatcggattc cgtttcaact ggcgctggct cctcgccctg
     2521 ctgggcatcg gcattgtttg cggaaccatt ctttactccc aattcctggc caaaaagata
     2581 gaacgggaag aacagcagaa gatagaacag tggctggccg ccagcaaagc cattctcaac
     2641 cccgccgatc tcacgctgcc caacctgatc cgcaacgaac agcattccat tcccattatc
     2701 gaaaccaacg aaaaagacag cataacaagc ttttttaacc tcgatacgat cgaggcaaaa
     2761 aaggacagat cctatttgcc agccaagctt aaaacattcc ggtcccaaaa tccgccgctg
     2821 gaaataaaat ggagtgattc cccgtacacc gcaaaccgct attactacgg acataccgcc
     2881 ctgcttgacc aggtacggta ttttcccata atccagcttt ttctggttgc catactcatt
     2941 actttcatta tttattccat cagcacccat aatcgttcta cccaaaacca ggtatgggca
     3001 ggcctggcca aggaaacggc tcatcagctg ggcacgccac tgtcttcgct ccagggctgg
     3061 attgaaatgt taaaggaaga aggtaccact gcggaaaccc tttccgaact ccagaaagac
     3121 ctggaccggc tgaaactcat ttccgaccgc ttctctaaga tcggcagcat tcctatcctc
     3181 gaaaaatcgg atatcgttcc ccagatacag caaatggtgg aatacatccg ccggcgtacc
     3241 acctccaaag tccagttttc cctggatgcc cacggaactt cagccgttga agcagatatc
     3301 tccaagccct tatttgactg ggtgatcgag aaccttttca aaaatgccct ggacgccatg
     3361 gaaggaaaag gtaagatcag cgtggatata aaaaatgaat cccagcaggt gatcattgat
     3421 gtgacggata cggggaaggg gatttctcca tccaatatcc ggcgggtctt taatcccggt
     3481 tttacaacaa aaaaaagagg ctggggactg ggactgacct tgtccaaacg gatcatggaa
     3541 gaatatcaca aaggcgacct cttcgtaaaa cattctgagc cggggaaagg caccacgttc
     3601 cggattgtac ttaaaaaata acgcaaattt gaacatccga aaatgacgga attgttaccg
     3661 ggaaaagcta tttttgcagt cccttttacc tcctgcggag gtggcgaaac tggtagacgc
     3721 actactttga ggtggtagcg ccttaaccgg cgtgggagtt cgaatctcct cttccgcacc
     3781 agggtaaagt cctgcaggta aattacttgc gggacttttt ttgctccggg tttcccgttt
     3841 cattgcgcac acttccccca ttgggcttcc tactccgttc tcaaactctt taccgggttt
     3901 gccatggccg cttttatggc ctggaagctc acggttatca atgcgatcag caatgcccca
     3961 aatgctgctg caaaaaatat ccaccaggaa atactaatgc gataggcaaa actttgcagc
     4021 catttattca tcccccacca ggcaacagga aatgcaatta aagatgcgat taaaaccagt
     4081 tttagaaaat ccctggaaag cgtccgggtt acctgagaga cacttgctcc caacactttt
     4141 cgtataccga tctcttttct gcgttgttca gccgtatagg tgaccagacc aaacaaaccc
     4201 agacaggcaa cgagaataat cagtacggaa aaaatgttga ggatctttcc cgttttctgc
     4261 tccgttgaat aggtattgtt aaagagatca tccataaaac tgcaggaaaa aggctcaccc
     4321 gttgcgtaac tatcccattg ctttttcatg gtggccaaca agccggcgat atccgtcgtt
     4381 tttattttaa agatcaaccc accttccgta tccaatctca taaataaagg gctcaccgct
     4441 tcatgtaagg atttgaaatt gaaattttta actaccccga tcacccgaaa cggaaggttt
     4501 gagccccggt cggtattttg tctaattatg gttttgccga tagcggatgc attattccat
     4561 ccaaatgcct ttgctgcggt ctcatttaaa atgattgcgg aagaatcggt tttaaagtct
     4621 ttggaaaatg ctctgccacc aaccattttc atgcccaggg tgggaatata ctcttcatcc
     4681 acatgatact ctactccatc caccagcaat ctgtcatttc cctgcggata agcgatggca
     4741 ttattataat ttgacggacc agccggttta tagaaggaca cggtagcatt tataatccgc
     4801 ggatcctgca gcatttgctg tttaaacacc tgctccctgt tccccaggaa ataagaattt
     4861 ggaatagtga ttagctggtc cttactgtat cccaggtcct tgttttgtat gtatttcatt
     4921 tgctggtaca cgacaatcgt tccgatgatc aacgcaacag aaataaaaaa ctggaatacc
     4981 accagaccac ttcttatacc aaagctcttg tggtccgatg cgagctttcc ttttaatacc
     5041 gctatcggct tgaacgagga taaatagaac gccggataaa ttccggcaac aaggccaacc
     5101 agcaatccca aaccaatgaa agcaagagta ggttttacat cgaaatgcaa atgctttccc
     5161 gaaatattat tgaatccggg catcgccagt ttcactaaca caaaggccac aatcaatcca
     5221 aaaaatgtaa tgagtatcga ctcggataag aactggctaa tgagctgaat ttttccggaa
     5281 ccagccacct tccttacccc cacctccttt gcccttttgg aagcgcctgc ggtggaaaga
     5341 ttaatgaaat tgatacaggc caccatcagc ataaaaacgg cgacgccccc aaaaatatac
     5401 acataggttg cattgcctcc gggttcaa
//
LOCUS       DASYCU010000499         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_253647, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000499 DASYCU010000000
VERSION     DASYCU010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253647"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VGM24_10025"
     CDS             complement(<1..621)
                     /locus_tag="VGM24_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4155756.1"
                     /translation="MSIEVKELTRHYGEQKAIDGVSFSLKQGEIAGFLGPNGAGKSTT
                     MKILAGCLKPSSGKAFINGIDTSEEPARSKKITGYLPESNPLYGDMYIREYLSFIADI
                     HRLPGKKKAVEEIIGVVKLRPEANKKIHQLSKGYKQRVGLAAALIHQPEVLILDEPTT
                     GLDPNQIVEIREVIREYGKERAVLFSTHILQEVEAICDRVIIINQGK"
     gene            complement(728..2053)
                     /locus_tag="VGM24_10030"
     CDS             complement(728..2053)
                     /locus_tag="VGM24_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEY4155757.1"
                     /translation="MKINAFLFRCTLVAALGGFLFGFETAVISGADKTIQQLWSLNSF
                     WQGFTIAASLIGTVIGAAIAGVPAQRWGRKRILSIIALMYLLSAIGCALTSTWILFVT
                     FRMIGGIAVGASSVIGPMYISELAPAKVRGRLAGMFQLNIVAGIFIAYLTNFLFVGLG
                     EDSWRWMLGIMAVPAGLFALLLRGIPESPRWLILNNRDAEAVAIFARTGEPDAGTLIR
                     EEHALSGRGTKESLFTGKFRKPILYAMLLAMFNQLSGINAILYYAPRIFEMAGFDKAQ
                     SYLQPVYIGAANLIFTFLAMTIIDKVGRKTLLITGATGMILFLALTAGVFNHSGQANS
                     MVLVYLLGFIAFFAFSQGAVIWVFISEIFPNSVRSQGGSLGSFTHWIMAAIISWTFPI
                     IVEGSPRGGFYSFIFYSLMTLASLIFIWRVMPETKGRSLEEIQKDLGIV"
     gene            2155..>2751
                     /locus_tag="VGM24_10035"
     CDS             2155..>2751
                     /locus_tag="VGM24_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY4155758.1"
                     /translation="MSFPYQIHSFEEYLSEYQKSIEQPEKFWSGIAENFTWKKKWDKV
                     LEWNFKAPKVEWFRGAKMNITENCLDRHLADKGDHPAIIWEPNDPAEKNRVLTYKELH
                     AEVCRFVQVLKNNSVKKGDRICIYMPMVPEAAIAVLACARMGAIHSVVFGGYSAQSIA
                     DRIDDAGCKLVITADGGHRGGKDILLKETMDEALQKTSQ"
BASE COUNT          716 a          697 c          702 g          636 t
ORIGIN      
        1 ctttccctgg ttaatgatga tgacgcgatc gcagatggct tccacttcct ggagaatatg
       61 cgtggaaaaa agcacagccc tttctttccc gtattcccgg atcacttcgc ggatctcgac
      121 aatctggttg ggatcgagac cggttgtcgg ttcatccagg atcaatacct ccggctggtg
      181 aatgagggcg gccgccagcc ccacgcgctg tttatatcct ttggaaagct ggtggatttt
      241 tttatttgcc tccggccgga gcttcaccac cccgatgatt tcctccactg cctttttttt
      301 acccggcaac cggtggatat cggctataaa ggaaaggtat tcgcggatat acatatctcc
      361 gtaaagcgga ttggattcgg gcaggtaacc agtgattttt ttggagcggg ccggctcttc
      421 actggtatcg attccgttta taaaggcctt gccgctgctg ggtttcaggc atcctgccag
      481 aattttcatc gtcgtggact tgcctgcccc gttgggcccc aggaatcccg cgatctctcc
      541 ctgcttcagg gaaaaagaaa ccccgtcaat ggctttttgt tccccatagt gcctggtgag
      601 ctcttttact tcaatggaca ttggcggagg gaaagttgat ggttgatatt gatcgttatt
      661 gaaactgagt tgcaaattta cgataaaacc tcgtcaacgg tcagcagtcg gccaaatccg
      721 ccgttcccta aacaataccc aggtctttct gtatctcctc gagacttcgg cctttggttt
      781 ccggcatcac gcgccagata aaaatcaggg aggctaaagt catcaggctg taaaatatga
      841 aagaataaaa tccgccccgg gggctgcctt ccacaatgat cggaaaggtc caggaaataa
      901 tcgcggccat gatccagtgg gtgaaactgc ccagggatcc gccctgcgaa cgaacggaat
      961 ttggaaatat ttccgagatg aaaacccaga tcacggcgcc ctgtgaaaag gcaaaaaaag
     1021 caataaaccc cagcaggtaa accaggacca tgctgtttgc ctgcccggaa tgattgaata
     1081 caccggcggt caatgccaga aaaaggatca ttcccgttgc accggtgatg agtaaggttt
     1141 tcctgccgac cttgtcaatg atggtcatcg ccagaaaagt gaagatcagg tttgccgctc
     1201 cgatataaac cggctgcagg taagattgtg ccttgtcgaa accggccatt tcaaaaatgc
     1261 ggggtgcata atataatatg gcattgatcc cggagagctg gttgaacatg gccagcagca
     1321 tcgcgtataa aatgggcttc cggaattttc cggtgaataa actttctttg gtcccccgcc
     1381 cggacaacgc atgttcttcc ctgatcaaag ttcctgcatc cggttctccg gttcgcgcaa
     1441 atatcgcaac cgcttccgca tcccggttat tcaggatcag ccagcgggga ctttccggaa
     1501 ttccgcgcag cagcagggcg aataatcctg ccggcaccgc catgatgccc agcatccatc
     1561 gccaggaatc ttctcccagg cctacaaaaa ggaaattggt gaggtaagcg ataaaaatac
     1621 ccgccacaat attcagctga aacatccccg cgaggcggcc acgaactttt gccggcgcca
     1681 gctcggagat atacatcggt ccgatcacgg aagatgcgcc aacggcaatt ccgccgatca
     1741 tcctgaacgt gacaaacagg atccaggtgg acgtaagtgc acagccgatg gcagacaata
     1801 aatacatcag ggcgataata ctcagtatcc ttttccggcc ccagcgctgt gcggggacgc
     1861 cggcgatggc tgcaccgatc acggtgccga tcaggctggc cgcaatggtg aacccctgcc
     1921 agaaagaatt cagagaccag agttgctgga tcgttttgtc agcaccggag atcaccgccg
     1981 tttcaaaacc gaataaaaaa ccacctaagg ccgctaccag tgtacagcga aaaaggaatg
     2041 catttatttt cattgctgcg ggtaatattt gaggatataa ggtagggaaa aaaatagtca
     2101 ttaaatttac ttcctgaaac agattccaaa ccttccatct tttaataacg ccgtatgagt
     2161 tttccctatc agatccattc tttcgaagag tatctttccg agtatcagaa aagcattgaa
     2221 caacctgaaa aattctggag tggaattgca gaaaatttta cctggaagaa aaaatgggac
     2281 aaggtgctgg aatggaactt taaagccccg aaagtggaat ggttccgggg agcgaaaatg
     2341 aatatcacgg agaactgcct ggaccggcat ctggcggata aaggagacca tccggccatc
     2401 atctgggaac cgaacgatcc ggcggaaaaa aaccgggtgt tgacctataa ggaattgcat
     2461 gccgaagtct gccgtttcgt acaggtgctg aaaaataatt cagtgaaaaa gggcgaccgg
     2521 atttgtattt atatgccgat ggttcccgag gccgcgattg ccgtactggc ctgtgcccgg
     2581 atgggcgcca ttcattccgt tgttttcggc ggatattctg cgcagagtat tgccgaccgg
     2641 atcgatgatg ccggctgcaa gctggtgatc accgccgacg gcggtcaccg cggcggcaag
     2701 gatatcctgc tgaaagagac catggatgaa gccctgcaga aaaccagcca g
//
LOCUS       DASYCU010000500         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4749071, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000500 DASYCU010000000
VERSION     DASYCU010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4749071"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="VGM24_10040"
     CDS             complement(<1..1708)
                     /locus_tag="VGM24_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4155759.1"
                     /translation="MSNRISFLLPVIALVFLGCHSADPYNSWGVVNGNPTGNKFSSLT
                     QIDTANVQGLQVAWTYHTGDVDTAAHSQIQCNPVIVNGIMYVVSPQLKLIALDAATGK
                     QIWTYSPFDSIVDKKTFFILNNCRGISYWSDGKEDERIFYTAGSFLRGINAKTGKLIE
                     SFGNKGMIDLREGLGRDVHDLLVTATSAATIYKDLILAGTRVSEGMDAAPGHIRAFNA
                     RTGKQEWIFHTIPHPGEPGYETWEDKDAWKLTGGANNWMGMVVDSKAGIAYVPTGSAS
                     MDFYGGKRRGADLYGDCLLALDAATGKLKWHFQYIHHDTWDWDPPSAPVLLTVKHDGK
                     TIDAVAQTTKTGFVFLFNRATGDPLFPILEKPVDTATGLAGEKLWPTQPVPQRPLPFV
                     RQTFSEKDINPYLSPEEYAEVKQQLSGYNKGMFTPQSKAGTVIFPGFDGGSEWGGPAV
                     DPGSRVLYVNANQMAWILKMFDVRNKPAKNENFGEAGLRLFKQNCMSCHGPDRKGSGN
                     YPSLLDINKKYDESSFTGFISAGRRMMPAFQYLKPEEKEALASFILENKKEQAKPFVP
                     ELT"
     gene            complement(1819..2859)
                     /locus_tag="VGM24_10045"
     CDS             complement(1819..2859)
                     /locus_tag="VGM24_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY4155760.1"
                     /translation="MLYVHKTSCFSPQQTFPETGLDRLTGSENNQLLVKEPVYSDIPA
                     GILRRMGKAVRMSVGAALPLLKEIPQPDGIIIGTANGGIEDCIKFLNQIIEYDEGLLT
                     PGNFVQSTSNAPAAQIALITKNKNYNSTKTHRGLAFENALLDAAMLLEEKPGDIYLVG
                     SVDEISSYNFNIDRLAGCFRSEPVSNRDLYDPVSEGTIAGEGALMIMVSDQSSGAVAR
                     LEALQTIHTTDEDLVLETLKKFLEKNGFPGTPVDLLITGENGDSRLEKYYRNAESLLP
                     ASTAVARFKHLSGEYCTASSFGFWLATEILRTKKIPETVLKKGGQTPCRKILFYNNYR
                     GLQHAFMLLSAV"
     gene            complement(2863..>3786)
                     /locus_tag="VGM24_10050"
     CDS             complement(2863..>3786)
                     /locus_tag="VGM24_10050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY4155761.1"
                     /translation="GLTPDQLASTETALVGATTVGGMCLTDEIFRDAHAKDSGTAYTS
                     SYDYASATLEAQKRFGVLGEISTINTACSSSANAILFGARLIKHGFAKRAIVGGADSL
                     AKFTINGFNALNILSKEICAPFDRDRNGLNLGEGAAFLVLEREEDVQGKRAYAVFSGF
                     GNSNDAFHPSSISPEGEGPYLSMKQALERAGLNAGEISYINAHGTGTENNDETESRAM
                     QRLFGEPPPFASTKSFTGHTLGASGAIEAVYSILSLRHQELYPSLQFKAPIPSTQFVP
                     VRHYREQEIQHVMSNSFGFGGNCTTLIFSKV"
BASE COUNT          823 a         1001 c          982 g          980 t
ORIGIN      
        1 ccgttaattc cggcacaaac ggttttgcct gctctttttt attttcaaga ataaaagagg
       61 caagggcttc tttctcctcc ggtttcagat actgaaaagc cggcatcatc cgcctgcccg
      121 cgctgataaa acccgtgaaa ctgctctcat cgtatttttt gtttatgtcc aggagcgacg
      181 ggtagttccc gctacccttc cggtcgggcc cgtggcagga catacagttt tgtttgaaca
      241 ggcgtaggcc ggcttccccg aaattttcgt ttttggcggg cttgttccgc acgtcgaaca
      301 tttttaagat ccaggccatt tgatttgcat tcacatataa aactcgggaa ccgggatcta
      361 cggcagggcc gccccattcg gatcctccgt cgaatccggg gaagatcacc gtccctgctt
      421 ttgactgggg cgtgaacatg cctttattat atccggacag ctgctgtttc acttctgcgt
      481 attcctccgg agacagatac gggttgatgt ctttttcaga aaaggtctgc cggacgaatg
      541 gcaggggcct ttgcggcacc ggctgcgtgg gccacaattt ttctccggcc agtccggtgg
      601 ccgtatccac gggtttttcc aggatcggaa acagcggatc tccggtggcg cggttgaaga
      661 gaaacacaaa cccggtcttt gtggtctgtg ccacggcatc aatcgttttt ccatcatgct
      721 tcaccgttag caaaaccggt gcagacggcg gatcccagtc ccaggtatca tgatggatat
      781 attggaaatg ccatttcaat ttcccggtag ctgcatccag tgccagcaga cagtctccgt
      841 agagatcggc gcctcttctt tttccgccat aaaaatccat ggaggccgat ccggtgggaa
      901 cataggcaat tccggccttt gaatccacga ccattcccat ccagttattg gcgccccccg
      961 ttaatttcca ggcgtccttg tcttcccagg tttcataacc gggttctccg gggtgcggaa
     1021 tagtatggaa gatccactcc tgtttccccg tacgcgcgtt gaacgcccgg atatgaccgg
     1081 gtgcggcatc catgccttca gatacccggg tgcctgccag gatgaggtct ttgtaaatcg
     1141 tcgccgcgga agtggccgtc accagcaggt cgtgcacgtc ccggcccagg ccttcgcgca
     1201 gatcgatcat gcccttattc ccgaagctct caatcagctt cccggtcttt gcattgattc
     1261 cgcggagaaa ggatccggct gtataaaata tccgttcatc ttcttttcca tccgaccagt
     1321 agctgatccc ccggcaatta ttcaaaatga aaaaggtctt cttgtcaaca atcgaatcaa
     1381 acggagaata ggtccagatc tgtttcccgg tggcggcatc cagtgcaatg agttttaact
     1441 ggggggagac aacgtacatg atcccgttta cgataacggg attacactgg atctgggaat
     1501 gcgcagcggt atccacgtcc ccggtatgat aggtccaggc tacctgcaat ccctgcacat
     1561 tggcggtatc tatttgggta agcgacgaaa atttatttcc ggtcgggttt ccgttcacca
     1621 ccccccagct gttatagggg tcggcggaat gacagcccag gaagaccagg gcgattacgg
     1681 gaagcaggaa ggagattcgg tttgacatgt aggagggtgt gagtatttag ataatttgaa
     1741 aattaagcaa tttgaaaatg atttcctttg gcaatgtatt ttcaaatctt cagattatct
     1801 aatttcaaat tttttctcct acaccgcgga cagcagcata aacgcgtgtt gcaatccgcg
     1861 gtagttgtta tagaacagaa tctttctgca cggggtttgc cctccttttt ttaagacggt
     1921 ttcgggaatc ttctttgttc gcaggatctc cgtggcgagc cagaatccga aggaggaagc
     1981 ggtgcagtat tcgccggaga gatgtttaaa ccgcgcaacc gccgtacttg caggtaaaag
     2041 ggattcggcg ttccggtaat atttttccag ccggctgtcc ccgttttctc cggtgatcag
     2101 cagatccacc ggcgttccgg ggaacccgtt cttttcaaga aattttttca gggtttccag
     2161 cacaaggtcc tcgtcggtcg tatgaatggt ttgtaatgct tccagccggg ctaccgctcc
     2221 ggaagactga tcggatacca tgatcatcaa agccccttcg ccggcaatgg taccttcgga
     2281 taccggatcg tacagatccc ggttggaaac cggttcactc cggaagcatc cggccagccg
     2341 gtcgatattg aaattataag aagaaatctc atccaccgat cccaccaggt agatatctcc
     2401 gggtttttct tccagcaaca tcgcggcatc cagcaaagca ttttcaaagg caagcccccg
     2461 gtgcgttttt gtactgttgt aatttttatt ttttgtgatg agggcaatct gcgcggccgg
     2521 tgcgttggac gtgctttgta cgaaattccc gggagtaagg aggccttcgt cgtattcaat
     2581 gatctggttc aaaaatttaa tgcagtcttc gatgccgccg ttggcggtgc cgataatgat
     2641 gccgtcgggt tgcgggattt ctttcagcag gggcaatgct gcccccacgc tcattctcac
     2701 ggccttgccc atccgccgta atatgcccgc cggaatatcg gaataaaccg gctcctttac
     2761 cagcaactgg ttattctccg acccggtcaa ccggtccaat ccggtttccg gaaaggtttg
     2821 ctgtggtgaa aaacaactgg ttttatgaac gtacagcatg attcagactt ttgaaaagat
     2881 aagcgtcgtg cagtttccgc cgaatccgaa ggagttggac atcacgtgct gtatttcctg
     2941 ctcgcggtaa tgccttaccg gcacgaattg tgtggagggt atcggcgctt tgaattgcag
     3001 ggaaggatac agttcctggt gccggaggct cagaatggaa taaacagctt ctatggcccc
     3061 ggacgcgccc agggtgtgtc ccgtaaacga ttttgttgaa gcaaaggggg gcggctcgcc
     3121 gaataaacgc tgcatggcgc ggctctcggt ttcatcattg ttctcggttc cggttccgtg
     3181 cgcattgata taactgatct ctcctgcatt taatccggct ctttccaatg cctgcttcat
     3241 ggacagatag ggaccctctc cttcggggga aatcgaagac ggatgaaacg catcattgct
     3301 gttgccgaat ccgctgaaca cggcataagc ccttttcccc tgcacatctt cttccctctc
     3361 caataccagg aaagccgcgc cttcccccag gttcaacccg ttgcggtcgc ggtcaaacgg
     3421 agcacaaatc tctttggaga gaatattgag ggcattgaaa ccattgatgg tgaatttggc
     3481 cagactatcc gctcctccca caatggcgcg tttggcaaat ccgtgcttga tcaaccgtgc
     3541 cccaaacaag atggcgtttg cagaggagga acaggcggtg tttatggtgc tgatctcgcc
     3601 caggaccccg aatcttttct gggcctccag cgttgcagaa gcgtaatcgt aagaggaagt
     3661 ataggccgtg cccgaatctt tggcgtgcgc gtcgcgaaag atctcatccg ttaggcacat
     3721 cccccctacg gtggtcgccc cgaccagcgc cgtttcggtg gaggccagct gatccggagt
     3781 caggcc
//
LOCUS       DASYCU010000501         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4166971, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000501 DASYCU010000000
VERSION     DASYCU010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4166971"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VGM24_10055"
     CDS             complement(<1..694)
                     /locus_tag="VGM24_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155762.1"
                     /translation="MHAVYRTKYILFISFIAALGGYLFGFDFAVISSALPFLRVQFGL
                     NAYWEGFSTACLAIGAIGGCVLAGRISQRFGRRSGLMCAAAVFAFSSVGMAFSPTLTY
                     FILFRFAAGFGVGMASMLSPVYIAEISPAKIRGRMVAVNQLAIVTGILVTNLVNYNIR
                     NLGEDAWRWMFGIGIIPSLFFFLGVFILPESPRWLLEKGRNPEAEKALSKIGNPHYIT
                     DTLNSIQNSLIRN"
     gene            complement(729..1610)
                     /locus_tag="VGM24_10060"
     CDS             complement(729..1610)
                     /locus_tag="VGM24_10060"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4155763.1"
                     /translation="MKTWKAELLYESNLILGEGAIWHPETRHFLYVDIEGKKLGSIDP
                     LNGISKEIQLDKKIGTVVPSAGGRLLVALQGSIEELNPGTGALRKLTDIEKDKKENRC
                     NDGKCDASGRLWIGTMNMDEKPHEGALYCYREKLEKKIGNVSVSNGICWSLNNDRMYY
                     IDSAAYNIRVYDFDAGSGNISNERILAEINELGFIPDGMTTDDQGNLWVAIWGGACVH
                     QYNAQTGALTGKVTVDALNVTSCAFGGEDYRQLFITTARTGLSETELEQYPLSGSLFM
                     INTGFRGLAPNYFKAEA"
     gene            complement(1636..2424)
                     /locus_tag="VGM24_10065"
     CDS             complement(1636..2424)
                     /locus_tag="VGM24_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4155764.1"
                     /translation="MPVELNFNGKTVLITGVTSGIGAAIAGLFARAGASVAGCGLPAG
                     DSLESARFISSASSQYAGALYVQADVTKEDQLKNLVDKTIGKFKKIDVLVSNAGANVF
                     EGAADCTEEEWLYNAELNLASHWRLAKMCKPWLERQKGVIILMSSNHAYSTIPGCFPY
                     SVAKTALTGLVRSLAIEWAPAIRTVGIAPGFIETAASQKWFGSFPEGEAERLRTIQLH
                     PVKRLGTPEEVGAWCVFLASDYASFASGTTYLLDGGRSALLQDS"
     gene            complement(2458..>2869)
                     /locus_tag="VGM24_10070"
     CDS             complement(2458..>2869)
                     /locus_tag="VGM24_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY4155765.1"
                     /translation="VGERHYTRHGIRPVLEKNICDVIMPDITRCGGPSEMKRMATMME
                     AYNVLLAPHNPNGPLSTIASAQVCAAVPNFFRQEYMFNDVEWRDRVITHPIKDMIRDG
                     HLELSERPGLGVDLVESEMEKHPGILTPKEGFYI"
BASE COUNT          714 a          763 c          657 g          735 t
ORIGIN      
        1 cattccggat caatgaattt tgtatactgt ttaaggtatc ggttatataa tgggggttgc
       61 cgattttaga cagggctttt tccgcctccg gattccttcc tttttcaagc agccatcggg
      121 ggctttccgg taaaataaaa actccaagga aaaaaaacag ggaagggatg attccgattc
      181 caaacatcca gcgccaggca tcttctccca ggtttcggat attataattg accaggttgg
      241 ttaccaggat cccagttacg atagcgagtt gattgacggc taccatccgg ccccttattt
      301 tggcgggaga aatttccgca atataaacgg gagacagcat ggatgccatt ccaactccga
      361 aacccgccgc gaaacgaaag agaataaaat aagtgagggt gggtgaaaag gccattccca
      421 ccgaagaaaa cgcaaaaaca gcagcggcgc acatcaagcc gctacgccgg ccaaaacgtt
      481 gtgagatcct tcccgccagc acacatcctc cgatggcgcc gattgccagg caggcagtgg
      541 aaaatccttc ccaataggcg ttgagtccga actgaaccct taaaaaaggc agggcactgg
      601 aaattacggc aaaatcaaaa ccgaataaat aacctcccag cgctgcaatg aaggagatga
      661 aaagaatata ttttgttctg taaactgcat gcatatcctg aatttgcagg tcttcttccg
      721 gtgttgcttt aagcctctgc tttaaaataa ttcggtgcca atccgcgaaa accggtgttg
      781 atcatgaaca gggagccgct taagggatat tgttccaatt ctgtttcact taatccggtt
      841 cttgccgtag tgataaataa ctgccggtag tcttcaccgc cgaaggcgca ggacgtaaca
      901 ttcagggcat ccaccgttac ctttccggtt aaagcaccgg tttgagcatt gtattgatgc
      961 acgcaggcgc cgccccagat ggcaacccac aaatttccct gatcatcggt cgtcattcca
     1021 tccggaatga agccgagctc atttatttct gccaggatcc gttcattgga gatattcccc
     1081 gaaccggcat caaaatcgta aacccggata ttgtaagcgg ccgaatcgat ataatacatc
     1141 cggtcgttgt tcagcgacca gcagatgccg ttggaaacac taacattccc gattttcttt
     1201 tccagttttt cccggtaaca atagagcgct ccctcgtggg gtttttcatc catattcatg
     1261 gttccgatcc acagccgccc ggaggcatcg catttcccat cattgcagcg gttctccttt
     1321 ttgtcctttt ctatatcggt cagtttccgg agagctcccg ttcccggatt caattcttca
     1381 atagaaccct gcaaggcaac aagtagacgg ccaccggccg aagggaccac cgttccgatt
     1441 tttttatcca gttgtatctc tttggagatc ccatttaacg gatcaatact gcccaatttt
     1501 ttcccttcga tatcgacgta tagaaaatgt cttgtttccg gatgccagat agccccttcc
     1561 cccaggatca gattcgattc atataataat tccgctttcc aggttttcat atctgtaaga
     1621 tggggtttaa tttacttagc tatcctgcag gagggcacta cggcctccat ccagtaaata
     1681 agttgttccg gaagcaaaag aggcgtaatc gctggccagg aaaacgcacc aggcacccac
     1741 ttcttccgga gttcccagtc gttttacggg atgaagctga atggttcgca gacgttccgc
     1801 ctctccttcg ggaaaagatc cgaaccattt ttgactggca gctgtttcaa taaagccggg
     1861 cgctatcccg accgtccgga tcgcgggcgc ccattctatg gccaggcttc tcaccaggcc
     1921 cgtcaatgcg gtttttgcaa cactataggg aaagcagccg ggaatggtgg aataagcgtg
     1981 attggatgac atcaggatga tcacgccctt ctgcctttcc agccagggct tacacatctt
     2041 cgccaaacgc cagtgcgaag ccaggttcag ttcggcatta tacaaccatt cttcttctgt
     2101 gcagtcagcc gctccttcaa atacatttgc tcccgcattg gataccagga cgtcaatttt
     2161 cttaaacttc ccaatcgttt tgtcgaccag gtttttcaac tgatcctctt ttgtcacatc
     2221 cgcctgaaca tagagcgcgc cggcgtattg tgaagaggcc gatgaaataa atcttgccga
     2281 ctccaggcta tccccggcgg gcagtccgca cccggctacc gaagcgcctg cccgggcaaa
     2341 caggccggca atggctgcgc cgataccgga agtaactccc gtgatcagca cggtcttgcc
     2401 attaaaattc aattctaccg gcataatact ttttctttac tcatggtccc ccggatttta
     2461 aatgtaaaat ccctctttgg gagtcaggat gccgggatgt ttttccattt cactttccac
     2521 gaggtcaacg cctaagcccg gcctttcact cagttccaga tgaccatccc ggatcatgtc
     2581 cttgatggga tgtgtgataa cccggtcgcg ccattccaca tcattgaaca tatattcctg
     2641 gcgaaagaaa ttgggaacag cggcgcatac ctgcgcgctg gcaatagttg acaaaggtcc
     2701 attcgggtta tggggggcca acaatacatt ataagcttcc atcatcgtgg ccatccgctt
     2761 catttccgaa gggccgccgc agcgggtgat atctggcata atgacatcgc agatattttt
     2821 ttccaggacg gggcggatac catggcgggt ataatgtctt tccccaacg
//
LOCUS       DASYCU010000502         6576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3878039, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000502 DASYCU010000000
VERSION     DASYCU010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3878039"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VGM24_10075"
     CDS             complement(<1..400)
                     /locus_tag="VGM24_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEY4155766.1"
                     /translation="MNKITLKTALPFVILVGISTLAIFVPSVANFDNGKYNGADVAWI
                     IVATALVFLMTPGLAFFYGGMVNRKNVISTMIKSLIAAGIVSILWIVVGYSLCFGQDV
                     NGLIGNPFTHFMFKGVNSGLPFASMPTVPLT"
     gene            complement(544..2277)
                     /locus_tag="VGM24_10080"
     CDS             complement(544..2277)
                     /locus_tag="VGM24_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4155767.1"
                     /translation="MDNDSLAPRINTRGVEANSFDAIVVGSGISGGWAAKELCEAGLK
                     TLVLERGRDVKHLKDYPTATLAPWEFPHRGEMPRKFVDENPLITKAAGFGEDTAHFFI
                     KDKDHPYVQEKPFDWIRGYQVGGKSLIWGRACPRWSRYEFTAPARYGYGIEWPIGYED
                     IAPWYSYVERFAGICGNRDGLEAMPDGEYLPPFEFNCVEKDLQKKLSSKYKDRFMIQG
                     RWAQLTQPADIHLKQGRARCQARDLCMRGCPFGAYFSSNSSTLPWAAKTGNLTIRPHA
                     VVHSIIYDENKKKAVGVRIIDAVTHQSTEYFARIIFMNASALNTNLILLNSVSGRFPN
                     GLGNDNGLLGKYVCHQNYRGSVNGRIEGFQDTYFYGRKPAESMMANFRNLKKQEMDFV
                     GGYMAFMGCYRRRAEQEPTRPQLGADFKEAISEAGEWGVYMYMQGETVPKETNQVYLS
                     KEEKDEWGIPLLVTNVSYDHNDELLLRDFLDQSAEMLSSAGVKDVEQTDNHWAPGRDI
                     HEMGGCRMGRDPKTSLLNGWNQLHHCKNVFVTDGACMTSTANQSPSILYMAFTARASG
                     FAVKELKKMSI"
     gene            2440..4836
                     /locus_tag="VGM24_10085"
     CDS             2440..4836
                     /locus_tag="VGM24_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY4155768.1"
                     /translation="MIKKNIFFLFILLSFQSMAQSLDSTNILFDAGWQFFKGGVEKGQ
                     EPALDDSHWRTLDLPHDWSIEDIGSTRSPFDSNAISQVGGGFTVGGTGWYRKRFTVPV
                     MEKGKRIHILFEGVYMNADVWVNGQHLGNHPYGYSSFWYDLTDKIKPGQENVLAVQVK
                     NEGQNSRWYSGSGINRHVWLQFLEPVHLVQWGTQVKSSEVSDSSARLGVSTAVKNTTD
                     KPASVQIISIILDPQGKEIARWKTNLELAPGAVSTERQTRRLPHPRLWSVSQPALYQL
                     RTEIYQQEHLLQQQQTPFGIRSIQFDAVNGFLLNGKSVKLKGGCYHDDNGPLGSRSYD
                     RAEERRVELMKASGFNAIRCSHNPPPPAFLNACDRLGMLVIDEAFDMWKDPKNPYDYH
                     LYFTDWWKRDISSMVLRDVNHPSVIMWSIGNEIPNREKPKVVDQARELAGYVRELDSS
                     RPVTSAVNDVREDKDPYFSVLDISGYNYAADHYEPDHQRKPDRIMMGTESYALEAFDY
                     WMPVVDHPYVIGDFIWTGFDYIGEASIGWRGYPQEKNFYPWNLAYCGDIDICGWKRPQ
                     SYYRDALWGADKLALFVHPPVPSFPVNPKTESWSKWNWDDVLADWNWTGFENKPMTVD
                     VYSSCEEVELFLNGKSLGKKPTNRNTKFRTSWTVPWQSGELKAVGFRNKKQIRQVSLF
                     SSGEPSQLQLSPDRKQIHSDGQDLTYVTVELLDRNHHIDPKAENAVHFSIEGPGTVVA
                     VGNANPVSTESYQLPGRKAWHGKCLVVLKSTTKAGNIVLHAKADGLPEAKVNIISKAD
                     "
     gene            complement(4833..6563)
                     /locus_tag="VGM24_10090"
     CDS             complement(4833..6563)
                     /locus_tag="VGM24_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY4155769.1"
                     /translation="MPADTLRINNRAAAQNTFDVIVIGSGISGGWAAKEFCDHGLKTL
                     VIERGRNVVHLKDYPTATKNPWDFPHRGEMPLSVSKNNPIVSRCYAFSEASQHFFVKD
                     QEHPYIQEKPFDWIRGYQVGGKSLLWARQTQRWSRYDFEGPGRDGFAVEWPVTYEEMA
                     PWYSHVEKFAGISGNRDGLDTLPDGEFLPAWEMNCVEKEISAKINRHYNNQRNVVQGR
                     CAHLTKPQPVHIEQGRNPCQARSLCERGCPFGGYFSSNSTTLPWAARTGNLTMRTDCV
                     VHSVIYDEQKKRASGVRVIDARTKEESTYFAKVIFVNAACLNSNLILLNSVSSRFPNG
                     LGNDSGLLGKYVCFHNYRGNVNASYDGPADRYYYGRRPTQPMMPNFRNVKKQEMDFLR
                     GYMVFFGAGRGRGNADAKDAEIGSAFKEDLTEAGPWYVSAMMQGETIPKEINHVRLSP
                     DRKDAWGMPELVISVGYDENDEKSLRDFLEQTSEILQVGGCTNIQANDSKQAPGLDIH
                     EMGGCRMGKDPKSSLLNKWNQLHHCPNVFVTDGAAMISTSTQNPSLTFMAFTARAANH
                     AVEELKKGNL"
BASE COUNT         1640 a         1624 c         1641 g         1671 t
ORIGIN      
        1 aggtgagcgg aacggtaggc atcgaggcaa aaggaagtcc ggaattcact cctttgaaca
       61 tgaaatgggt aaatggattt ccgatcagac cgttgacgtc ctgtccgaaa cagaggctgt
      121 aacccaccac gatccagagg atgctgacaa tgccggcagc gatcaggctt tttatcatgg
      181 tggagatcac gttcttccgg ttaaccatgc ccccataaaa aaaggccagt cccggagtca
      241 tcagaaatac cagtgccgtg gctacgatta tccaggcaac atccgccccg ttgtatttgc
      301 cattgtcaaa attggctact gagggaacaa agatggccag agtagagatt ccaaccagga
      361 ttacgaacgg taaggcagtt tttaaagtga ttttgttcat gctggggcag ttttgcaaat
      421 tatttttaaa aaatatttcc gctaaccccc gcaaattaga taaataaccg attaaaatat
      481 atatttattt tattttaatg tatatgctca gatattttaa ataattattt atgaaaacag
      541 gccttaaata ctcatttttt ttagctcttt cacagcaaaa cccgacgcgc gcgctgtaaa
      601 agccatatat aatatcgaag gactctgatt tgccgtactg gtcatgcagg ctccgtctgt
      661 gacgaagacg tttttgcaat ggtgtagctg gttccatccg tttagcaggg aagttttcgg
      721 atccctgccc atccggcatc ctcccatttc atggatatcg cgccccggag cccagtgatt
      781 gtcggtctgc tcaacatcct tcacgcctgc actggataac atttcagccg attgatccag
      841 gaaatccctg agcaggagtt catcattatg atcataagaa acgttggtaa caagcagcgg
      901 aatgccccat tcgtcttttt cctctttgct cagatacacc tggttggtct ctttaggtac
      961 cgtttctccc tgcatataca tataaacgcc ccactccccc gcctccgata tggcttcctt
     1021 aaaatcagcg ccgagctggg gcctggtcgg ctcctgttcg gctcttctcc ggtagcatcc
     1081 catgaatgcc atataccctc ccacaaaatc catttcctgc tttttcaggt ttctgaaatt
     1141 ggccatcatg gattcagcgg gtttgcggcc atagaaataa gtatcctgaa atccctcaat
     1201 ccttccattt accgaaccgc gataattctg gtgacaaaca tatttaccca ggagtccgtt
     1261 gtcgttgcct aatccgtttg gaaaccggcc ggaaacggaa ttcagcagga tcaggttggt
     1321 attaagggcc gaagcattca taaaaatgat ccgcgcaaaa tattccgtgc tctgatgggt
     1381 aacggcatca atgatgcgaa cacccacggc tttcttttta ttttcatcat aaataatgga
     1441 atgaaccacc gcatgcggac ggatagtcag atttcctgtt ttggcagccc agggaagggt
     1501 ggatgaattg gaactgaaat aggcgccgaa cgggcatccg cgcatgcaga gatcgcgggc
     1561 ctggcagcgc gcgcgcccct gctttaaatg gatgtcggcc ggttgggtca gctgggccca
     1621 tcggccctga atcataaacc ggtctttgta ttttgaactc aatttttttt gcaggtcctt
     1681 ttccacgcag ttaaactcga atggcggaag gtattctcca tccggcatgg cttccagtcc
     1741 gtcccggttt ccacagatac cggcaaaacg ctccacgtaa ctgtaccagg gcgcaatatc
     1801 ttcgtaaccg atgggccatt caatgccata tccgtaacgg gcgggcgcag taaattcata
     1861 ccggctccag cgcggacagg ctcttcccca gatcagggat tttccgccga cctggtaacc
     1921 cctgatccag tcaaacggtt tttcctgaac gtagggatga tccttgtcct taataaaaaa
     1981 atgagccgta tcctcgccga atccggcagc cttggtgatg agcggatttt catccacaaa
     2041 ttttcttggc atttccccgc ggtgaggaaa ttcccaggga gccagcgtgg ccgttggata
     2101 atccttgaga tgctttacat cccggcctct ttccaggacc agcgttttta gtccggcctc
     2161 acaaagctct tttgccgccc agcccccgct gatcccggaa ccgactacaa tggcgtcgaa
     2221 ggaattggcc tccactccgc gggtgttgat acgtggagca agcgaatcgt tatccataag
     2281 ctgattttac ggaacaattt acacaatcct gaaaagtaaa aagtaaaatg taaaaagggc
     2341 gcggaggcgc tacccggact ttatgaaacc ctgataaatg tttaattaac ccttatttta
     2401 tttattttta gagcggatat gaaattttta cccccggcca tgataaaaaa aaatattttt
     2461 tttctgttta ttctgctcag ctttcaaagt atggctcaat cgctggattc aacgaatatt
     2521 ttatttgatg ccggatggca gtttttcaaa ggaggtgttg aaaaaggcca ggagcccgcg
     2581 ctggatgaca gccactggcg aaccctggat ctgccgcacg actggagcat agaggatatc
     2641 ggatcaaccc gttccccttt tgattccaat gcgatcagcc aggtcggtgg cggatttacg
     2701 gtgggcggca cgggctggta ccgcaaaaga tttacggtgc cggtaatgga aaaggggaaa
     2761 agaattcata ttttatttga aggtgtgtac atgaacgccg atgtctgggt gaacggacag
     2821 cacctgggaa accatccgta cggttacagc agtttctggt atgatctgac cgataaaata
     2881 aaacccggac aggaaaatgt gctggcggtc caggtgaaga acgaagggca aaacagccgc
     2941 tggtattccg ggtcagggat taaccgccac gtctggctgc aatttctgga acccgttcac
     3001 ctggttcaat ggggaaccca ggttaagagt tcagaagtgt cggatagttc tgcccggctt
     3061 ggcgtcagca cggctgttaa aaatacaacg gacaaaccag cttcggttca aatcatatcc
     3121 attatcctgg atccgcaggg aaaggaaatt gccagatgga aaacaaacct ggagctggcg
     3181 cccggcgcgg tttcgactga gcggcagacc cgtcgcctcc cgcatccgcg gctatggtca
     3241 gtcagccagc ccgccttata ccagcttcgc acggaaattt atcagcagga gcacctgctg
     3301 cagcaacagc aaacgccttt cggaatccgc agcattcagt ttgacgcggt aaacgggttt
     3361 ttgctaaacg gaaaatcggt aaagttgaaa ggaggctgct atcatgacga caacggcccg
     3421 ctaggctcca ggtcgtacga ccgcgcggaa gagcgccggg tggaactgat gaaagccagc
     3481 gggttcaatg cgattcgctg ttcgcataat cctcctcccc ctgccttcct gaatgcctgt
     3541 gaccgtctgg gcatgctggt gattgatgaa gcattcgaca tgtggaagga tcccaagaat
     3601 ccgtatgatt atcatttata ttttaccgac tggtggaaaa gagatatttc atccatggtg
     3661 ctgagggatg tgaatcatcc ttccgttatc atgtggagca tcggaaatga aatccccaac
     3721 cgggaaaaac cgaaagtagt ggatcaggcc agggaactgg cgggttatgt gcgggaactc
     3781 gattccagcc ggccggtcac ttctgccgta aacgatgtgc gggaagacaa ggatccctat
     3841 ttttccgtac tggatatttc gggatataat tatgcggcgg atcactatga accggatcat
     3901 cagcggaaac ccgatcgcat catgatggga acggagtctt atgcgctgga agccttcgat
     3961 tactggatgc cggtagtgga tcatccgtat gtgatcggag attttatctg gaccggtttc
     4021 gattatatcg gcgaagccag tataggctgg agaggatacc cccaggaaaa aaacttttat
     4081 ccctggaatc tcgcgtattg cggggatatt gatatctgtg gctggaaacg gccgcaatcc
     4141 tattaccggg atgcgctttg gggcgcggat aaactggctc tttttgtgca tccgccggtt
     4201 ccttcttttc ctgtaaatcc gaaaacggaa agctggagta aatggaattg ggatgatgtg
     4261 ctggccgatt ggaactggac cgggtttgaa aataaaccca tgaccgtgga tgtttattct
     4321 tcctgcgaag aagtggagtt atttctcaac ggaaaatctt tggggaaaaa gccaaccaac
     4381 cggaatacga aattccgtac ctcctggact gttccctggc agagcggaga attaaaggcc
     4441 gttggcttca gaaacaaaaa gcaaatccgg caggtttctc tattcagttc aggagagccc
     4501 tcgcaattgc agctttcgcc ggacagaaaa cagattcatt ccgatgggca ggatctgact
     4561 tatgttactg tggagctgct ggacaggaac catcatattg atccgaaagc agaaaatgcc
     4621 gttcactttt caatagaagg acctggcacc gtggtcgccg taggaaatgc caatccggtc
     4681 agtacggaaa gctatcagct gcccgggcgg aaagcctggc acggcaaatg cctggtagta
     4741 ttgaaatcca ctacaaaggc tggaaatatt gtgctccacg caaaggcaga cgggctgccg
     4801 gaagcaaagg tgaatattat ttcaaaagcg gattagaggt ttcctttctt taattcttcg
     4861 accgcatgat tggcggcccg ggcggtgaac gccatgaatg ttaaggatgg gttctgggtg
     4921 ctcgtggaaa tcattgcagc cccatccgta acaaaaacat tcggacaatg atgcaattga
     4981 ttccatttat ttaagagaga agatttggga tccttcccca tacggcatcc gcccatttca
     5041 tgaatatcca gtcccggcgc ctgtttgctg tcgtttgcct ggatattcgt acagccgccc
     5101 acctgaagta tctccgatgt ctgttctaga aaatcccgga gtgatttttc atcattctca
     5161 tcataaccca cggaaatgac cagttccggc atcccccagg catccttccg gtcgggactc
     5221 agccggacat gattgatttc tttcggaatg gtttccccct gcatcatggc ggatacatac
     5281 cagggccccg cttccgtaag gtcttcttta aatgccgaac cgatctccgc gtctttggca
     5341 tcagcgtttc ccctgccccg gcctgcgccg aaaaaaacca tatatccgcg gagaaagtcc
     5401 atctcctgtt ttttcacatt ccggaaattg ggcatcatgg gctgggtcgg tctgcgacca
     5461 taataatatc gatcggccgg tccgtcatag cttgcattta cattgccccg gtaattatgg
     5521 aaacaaacgt atttgccgag cagaccgctg tcatttccca aaccattggg aaaacgggag
     5581 gatacggaat ttaataagat caggttggaa ttcaggcagg cggcatttac aaaaatgact
     5641 tttgcaaaat aagtgctctc ttcttttgta cgggcatcga tcacgcggac tccggatgct
     5701 ctttttttct gttcgtcgta aatgaccgaa tgtaccacgc agtctgtccg catcgtgagg
     5761 ttcccggttc ttgctgccca ggggagcgtg gtggaattgg aactgaaata accgccgaag
     5821 gggcagccgc gctcgcaaag acttctggcc tggcagggat tacgtccctg ctctatatga
     5881 accggctggg gtttggtcag gtgggcgcat cgtccctgca ccacattccg ctggttatta
     5941 taatgacggt tgatttttgc ggagatctct ttctcaacac aattcatttc ccaggcgggc
     6001 aaaaattcgc cgtcgggaag tgtatccaaa ccatcgcggt tgccgctgat accggcaaat
     6061 ttttcaacgt gcgaatacca gggcgccatc tcttcgtagg taacgggcca ttcaacggca
     6121 aacccgtcgc ggccgggtcc ttcgaaatcg taccggctcc agcgctgggt ctgtcttgcc
     6181 caaagcaatg acttcccgcc gacctggtag ccacggatcc agtcgaatgg tttttcctgt
     6241 atatatggat gttcctggtc cttgacaaaa aaatgctgcg aggcttcgct gaatgcataa
     6301 cagcggctga cgatgggatt gtttttgctg acggaaagcg gcatttcgcc ccgatgcggg
     6361 aaatcccagg gatttttagt ggctgtcgga taatccttaa gatggaccac atttcttccg
     6421 cgttcaatga ccagcgtctt caggccatga tcacaaaatt ctttggcggc ccagccgcca
     6481 ctgatacccg atccgataac aatgacgtca aacgtatttt gggcagcagc ccggttattt
     6541 attcggagtg tgtcggctgg catatcggtt tcaatt
//
LOCUS       DASYCU010000503         4654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3299138, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000503 DASYCU010000000
VERSION     DASYCU010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299138"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VGM24_10095"
     CDS             complement(<1..1383)
                     /locus_tag="VGM24_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY4155770.1"
                     /translation="MGKASLLSALIFFCLQTTAQPAKNGNQILKNDWKMQSSLAVSAA
                     GPEISQTSFRESGWYPVSLPTTIIAGLVKNKRYDFDPFYADNLKKIAGPEFDRPWWFR
                     KTFVLPASEKGKNVTLILHGINYRANIWLNGVEIADSNHVIGPFRIFEFDVTPQIKYG
                     SPNVLALEVTRPFNPNRRGGDLAIDYADWIHYPSDYNGGVVNDVEISTCDKIAVRHPL
                     VTTKFDLPSLSTAHLQVDAELTNYSDKAESAIIKGKINEDISFQQEVHLAPGESKDLS
                     FSPADFPQLNVKNPRIWWPWQYGRPLLNTIKLEVIRNGKSGNFVAERFGIREVSSEMI
                     DSTARKFIINGKPILLRGAAWSPDIFQRRSAERQEQEIKLVRDMHMNIIRSEGKLEDD
                     HFYELCDQYGLLVMTGWMCCGAWQYPEKWDSVKRNVAMESAKSVMYWLRNKACIFVWL
                     NGSDMPPRDTT"
     gene            1506..2771
                     /locus_tag="VGM24_10100"
     CDS             1506..2771
                     /locus_tag="VGM24_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEY4155771.1"
                     /translation="MKRNYYIVSLILLTFFVISFLTNVIGPLIPDMINGFHLSLTLVA
                     LLPFAFFIAYGVMSIPSGMLIEKYKEKKIMITGFVVAFAGSLLLAAFPNYLTAVLSLF
                     LIGCGMAMLQVVINPLLRTSGGEEHYAFNSVLAQLIFGLASFISPLVYSYLVQNLKGG
                     STGHGFASFLSGLVPASLPWISLYWLFAVICLLMILIILFSKFPKVELSKEEVVGAWQ
                     THVMLFKNPVVILFFIAMFCYVGTEQGVANWISQFLSVYHGYDPQTTGAQAVAYFWGL
                     MTAGGVVGLLLLKFIDSRKVLILFTVLALVCLSLALFGPAQVSLVSFTLVGFFASVMY
                     PVIFSLALNSVKEHHGSFSGILVTGIIGGAVIPLIIGWLGDHLGLRSGMFFLYLTMGY
                     ILSIGFWAKPIITNKTVRLFRKKDPEENA"
     gene            2772..3632
                     /locus_tag="VGM24_10105"
     CDS             2772..3632
                     /locus_tag="VGM24_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY4155772.1"
                     /translation="MKNSTEEYVIGIDLGGTNVRSGRVSENAVTGIHSKRINNQASVE
                     EVLQDIFSITDPLISPSVKAIGIGVPSVVDLEEGIVYDVQNIPSWKEVPLKKWMEEKY
                     QLPVIVNNDANCFALGEYYYGKGKGRLSMIGLTIGTGLGAGIIIHNKLYAGANCGAGE
                     FGMVEYLDKCYEYYSCGQFFQNVYQLDGEEVFKKAKAGDAKAISLYGEMGTHLGNAIK
                     TMLYTYDTDLIILGGSVRKAFPYFSGNMWKRIHTLVYPRSANRLRIELSELENSGILG
                     AAALYYDSIR"
     gene            complement(3818..>4654)
                     /locus_tag="VGM24_10110"
     CDS             complement(3818..>4654)
                     /locus_tag="VGM24_10110"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155773.1"
                     /translation="IVSKGTVDALLAKLYATRSPANWDSVAYYCDQVIPHYSLLSDYS
                     YLWDNNHKNNSEAIWELPFDGYSVGDQVGNWAPSIWVGGSKGAYEGGGWKKFNTPTND
                     LVNAFLSENDNVRLNNSITFLDITGQWSDPYWTDYTHYPFLTKYNDPANGLNDMYLIR
                     LADILLLRAEAYNNSSDIPDAASLVNQVRARVGLPATTAASQADMALAIEKERRLELA
                     FEGQRWFDLLRTGRALDVMNAQKDGNGNSLNYNVQPYQLLLPIPQTQLDLNPLLTQNP
                     GY"
BASE COUNT         1089 a         1117 c         1167 g         1281 t
ORIGIN      
        1 ggtcgtgtcc ctgggcggca tgtcacttcc attcagccag acgaaaatgc aggccttgtt
       61 tcgcagccag tacatgactg atttcgccga ttccatggcc acatttcttt ttacactgtc
      121 ccatttttcg gggtattgcc aggcgccgca acacatccat ccggtcatga ccagcaaccc
      181 gtactgatcg cacaattcgt agaaatgatc gtcttccagt tttccctccg aacggatgat
      241 attcatgtgc atgtcgcgga ccagcttaat ttcctgctcc tgcctttctg ccgaacgcct
      301 ttggaaaata tccggagacc aggcggcgcc gcgcaacagg atcggcttgc cgttgatgat
      361 aaatttcctg gccgtggagt caatcatttc ggaactgacc tcccggatcc caaaacgctc
      421 ggctacgaaa tttccggatt taccgtttcg gatcacttcg agcttaatcg tattcaataa
      481 tggacgccca tattgccagg gccaccagat gcgcgggttt ttcacattta actgggggaa
      541 atccgccggc gaaaaactca ggtctttgct ttcgcccggt gccagatgca cctcctgctg
      601 aaagctgata tcctcgttta tttttccttt aatgatggcg ctctccgcct tatccgaata
      661 attggtcagc tccgcatcca cctgcagatg cgccgtggaa agggaaggca ggtcgaattt
      721 tgttgtcacc agcggatgac gcacggcaat tttatcacag gtgcttattt ccacatcatt
      781 caccacccct ccgttataat ctgaaggata atggatccag tcggcataat caatggccag
      841 gtctccgcct ctgcggttcg gattgaatgg acgggtcact tcaagcgcca atacattggg
      901 gctgccatat tttatttgcg gggttacgtc aaattcaaag atccggaaag gaccgatcac
      961 gtgatttgaa tcggcaatct ctacaccgtt cagccagata tttgcccggt agttgatgcc
     1021 atgaagaata agcgttacgt ttttcccctt ttctgaggca ggtaaaacaa acgttttcct
     1081 gaaccaccaa gggcggtcaa attcaggtcc tgcaattttt ttcaggttat ccgcgtaaaa
     1141 cggatcaaaa tcgtagcgtt tgtttttaac gagaccggcg atgatagtgg taggcaggct
     1201 cacgggatac catccggatt cccggaaaga agtttgggag atctccggcc ctgccgcact
     1261 aacggcgagg gacgactgca ttttccagtc attcttcaga atctgatttc cgtttttagc
     1321 cggttgggcg gttgtctgaa ggcagaagaa gatcagagcg gatagcaagc tggcttttcc
     1381 catggtatgg cagttaagag aactaagtta caaatttaaa gatagcctgc ctttgccggt
     1441 cttgggtaaa cttgatatat ttataggcca atcccatcca gccactttaa gccagacttg
     1501 aatttatgaa acgcaactat tatatcgtaa gccttatcct gcttaccttt ttcgtcattt
     1561 cctttctcac gaatgtgatc ggacccctga tcccggatat gatcaacggg tttcacctca
     1621 gcctcacgct ggtggcgctt ttgccatttg cttttttcat cgcgtacgga gtcatgtcga
     1681 tcccttccgg gatgctgatt gaaaaataca aagaaaaaaa gatcatgatc acggggttcg
     1741 ttgtcgcctt tgccggttcc ctcctgctgg cggcatttcc gaattacctg acggcggtgc
     1801 tttctctttt cctgatcggc tgtggaatgg cgatgttgca ggtggtcatc aatccgctgc
     1861 tgcgaacctc cggcggagag gaacactatg cctttaattc ggtgctggcc cagttaatat
     1921 tcggactggc ttcctttatc agtcccctgg tatattccta tctcgtacaa aatctgaaag
     1981 gcggcagcac gggccatggt tttgcttctt tcctgagcgg tcttgttcct gccagcctgc
     2041 cctggatatc cctgtactgg ctttttgcgg tgatttgcct gctcatgatc ctcatcatcc
     2101 tgttttcgaa atttccgaaa gtggaattga gcaaggagga agtggtcggg gcatggcaga
     2161 cccatgtgat gttgtttaaa aatccggtgg tcatcctgtt ttttatcgcc atgttctgtt
     2221 atgtgggcac ggaacagggg gtcgccaact ggatttcgca gtttttatcg gtttatcacg
     2281 gatatgatcc ccaaacgacc ggagcacagg cggtggctta tttctgggga ctgatgacgg
     2341 caggcggagt ggtcggtttg ctcctgctca aatttattga cagccgcaag gtcctgatcc
     2401 tttttacggt gttggcgctg gtctgcctga gcctggcatt attcggtcct gcccaggttt
     2461 ccctggtctc ttttaccctg gtgggatttt ttgcttccgt gatgtacccg gttatttttt
     2521 cactggcact gaattccgtc aaggaacatc atggatcttt ttccggaata ctggtgaccg
     2581 gtataatcgg cggggcggtg attccattga tcattggctg gctgggggat cacctgggcc
     2641 ttcgctccgg catgttcttc ctgtacctga ccatgggata tattttaagt atcgggttct
     2701 gggcaaagcc catcattaca aataaaacgg tccgcctgtt ccgtaaaaag gatccggaag
     2761 aaaatgcata aatgaaaaat tcaacggaag aatacgtgat cggcattgat ctgggaggaa
     2821 ccaacgtgcg tagtggccgg gtaagtgaaa acgcggtgac cggcattcat tccaaacgga
     2881 ttaataacca ggcatccgtg gaggaagtat tgcaggatat tttttccata accgatccac
     2941 tgatcagccc ttcggtaaaa gccataggga tcggcgttcc cagtgtcgtg gacctggagg
     3001 agggcattgt atatgatgtg cagaatattc cttcctggaa agaagtaccg ctgaaaaaat
     3061 ggatggagga aaaataccag ttgccggtga tcgtgaataa tgacgccaat tgttttgcgc
     3121 tgggtgaata ttattatggc aaagggaaag gccgtttgtc tatgatcggg ctgacgatcg
     3181 gaaccggttt gggcgcaggc atcatcatcc ataataaatt atatgccggg gctaattgcg
     3241 gcgccgggga attcggcatg gtcgaatacc tggataaatg ttatgaatat tattcctgcg
     3301 gccaattttt tcagaatgta tatcagttag acggtgaaga agttttcaaa aaggcaaaag
     3361 ccggtgatgc gaaagccatc agcctgtatg gagaaatggg cacacacctt ggtaatgcca
     3421 tcaagaccat gttgtatacc tatgatacgg acctgatcat cctgggcgga tctgtccgta
     3481 aagccttccc ctatttttcc gggaatatgt ggaaacggat tcacacgctc gtgtatccgc
     3541 gttctgcgaa ccggctccgg atagaattgt ccgaacttga aaacagcggc atcctcggag
     3601 ccgctgccct gtactacgat tcgattagat aatcctgctt taattaattt gtttttttta
     3661 agatcttccg gtcaaatttc atggtgactt agtacgcctg accagcctct tctccggaaa
     3721 tgtgacttca aacggcggcg acctgatttc aaaagcggaa aaataaaaaa ggctgcctca
     3781 tactatggag acagcctttt actaatcaga gccagcctca ataacccgga ttctgggtta
     3841 ataaaggatt taaatcaagc tgggtttggg gaatagggag taataactgg tatggctgta
     3901 cattataatt caggctgttg ccgtttccgt ctttttgtgc attcatcaca tccagtgccc
     3961 ggcctgttct cagcaggtcg aaccatcgtt ggccttcgaa cgccagttca agccgtcttt
     4021 ctttttcaat ggccagggcc atatccgcct ggctggcagc cgtggttgcc ggaaggccca
     4081 cccgggctct cacctggttg accaggctgg cagcatccgg aatatccgaa ctgttattat
     4141 aggcttctgc ccggagcagt aaaatatcgg caaggcggat caggtacatg tcattgagcc
     4201 cgtttgccgg atcgttgtac ttggtgagaa aggggtagtg ggtgtagtct gtccaatagg
     4261 gatcgctcca ctgaccggta atatccaaaa aggtaatgga attattgagc cgtacattat
     4321 cgttttcaga aagaaaggca ttgaccaggt cgttggtggg ggtattgaat tttttccatc
     4381 cgccgccttc ataagcaccc ttggatcctc cgacccagat agaaggggcc cagttgccca
     4441 cctggtctcc cactgaataa ccgtcgaaag gcaattccca gatggcttca ctgttattct
     4501 tgtgattgtt atcccagagg taactgtaat cggacagcag ggaataatgc ggtatgacct
     4561 ggtcgcaata ataggcaaca gaatcccagt tcgccggcga tctggtggca tacagcttgg
     4621 ccagcagggc atcaacggtt cctttggaaa caat
//
LOCUS       DASYCU010000504         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4889814, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000504 DASYCU010000000
VERSION     DASYCU010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4889814"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..688
                     /locus_tag="VGM24_10115"
     CDS             80..688
                     /locus_tag="VGM24_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155774.1"
                     /translation="MERLREIETELSGIAPVLGKNGITLPPYRVPAGYFEHFAEGLLQ
                     KIREENPDTELSALSPLLAGIERRTPYRVPDQYFNEFSAQTASSAKPKASIIALRSGS
                     RSWMRYAAAAILTGAIATATFFSFHKTNSDPLSGLNKMSDQEMASYLENQDVHWAPGT
                     STTPTASIDFDDNDISDLLSNVSDAELEQYLPDLPVKKRTIN"
     gene            693..1091
                     /locus_tag="VGM24_10120"
     CDS             693..1091
                     /locus_tag="VGM24_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155775.1"
                     /translation="MKKFILYTFFVLAGYGLQAQNGNRLEALKIAYITKKMDLSPEEA
                     QRFWPIYNQYAAELKQARQEALRNNQTEIDLDETQLRIRKKYNSQFSQVISPQKVNIF
                     FKSEKEFGVYVQKEMERRELRAQKRNGNRP"
     gene            1407..2663
                     /locus_tag="VGM24_10125"
     CDS             1407..2663
                     /locus_tag="VGM24_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HEY4155776.1"
                     /translation="MKKSYVFILVLFTLPGLLMAQDTAQQIIPGRTNQESQLNKPYVI
                     LISADGFRYDLADKYQAKNLLKFRKQGVNADYMQSSFPSLTFPNHYTIVTGLYPAHHG
                     LVDNSFYDQSKNEYYSLGNKAAVRDSSWYGGTPLWVLAEKAGMLTASFYWVGSEAAIQ
                     GVRPTYYYNFNNLIPMDTRIGEVRSWLELPEEKRPHLIFFYIPDVDHQEHLHTADSKE
                     TEKAVHFVDESIGKLVRSVDSLHLPVDFIFVSDHGMTTVDTAHGIELPAALDTNQFRI
                     VNSLTLVHLYAYNQKDVLTTEIALKSQATDYNVYLPEEIPAEWHYSKTDDWYNRIGDI
                     VLVSHPPKVFNFKKRRINPGNHGYDPSLTEMHACFYAWGPDFKKHYQIPGFENVNVFP
                     LIAKILRLRYSDPIDGHLRVLKKTLR"
     gene            complement(2670..3425)
                     /locus_tag="VGM24_10130"
     CDS             complement(2670..3425)
                     /locus_tag="VGM24_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY4155777.1"
                     /translation="MRIIPLSEGAFTVGKTKEFIPFEPGQDDLQKRAPGSLLVEIQPF
                     VVITRNDVLLLDTGLGFEKNGELQLHQLMKTAGIDPDSVTKVLMSHLHKDHVGGMGKK
                     APDGGRVAAFPQAKYYIQQRELEYAIEKGPPSYEPELFAFLNSFSNLVKLEGNGKIDD
                     YIRFELTGGHCPFHQVFHLEEDGQHLFFGGDVAPQLNQMKSRFVAKYDYDGKKCMLLR
                     QQWWDQGREEKWTFLFYHDIKTPVFEFENQANR"
     gene            complement(3497..>3841)
                     /gene="rodA"
                     /locus_tag="VGM24_10135"
     CDS             complement(3497..>3841)
                     /gene="rodA"
                     /locus_tag="VGM24_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY4155778.1"
                     /translation="FVPEQRTDFIFCTIGESFGFTGSVIFLCIYGILLMRIVTIAERQ
                     RSVFSRAYAYGVASVFFFHIAINICMTIGLAPVIGIPLPFVSYGGTSLLTFSVMLFIL
                     IRLDSDRQMVLR"
BASE COUNT         1065 a          962 c          866 g          948 t
ORIGIN      
        1 catcacgcag tgaaaaaaat tgaagatttt attctgaatc attaaacttt ggagccctga
       61 aagggtctta ttggaaccca tggaaaggtt aagagaaata gaaactgagt tatccgggat
      121 cgcaccggtt ctgggtaaaa acggaatcac gttaccgcct tatcgtgttc cggcaggcta
      181 ttttgagcat tttgcggaag ggctgcttca aaaaatccgg gaagaaaatc cggacacgga
      241 actgagcgcg ctttccccat tacttgccgg tatcgaacgc aggaccccat accgggtccc
      301 cgatcagtat tttaatgaat tcagtgccca aacggcttca tccgccaaac caaaggccag
      361 cattattgcc ctgcgctccg gcagcaggtc ctggatgagg tatgcggcgg ccgcaatact
      421 taccggcgcg atcgcaacag ccactttctt cagtttccat aaaaccaaca gcgatccatt
      481 aagcggtttg aacaaaatga gcgatcagga aatggcgagt tacctggaaa atcaggatgt
      541 tcactgggct cccggaactt caaccacgcc gaccgcttcg attgatttcg atgacaatga
      601 tatcagtgac ctgctgagta atgtttcgga tgcggagctt gaacaatacc ttcccgattt
      661 gccggttaaa aaacggacaa taaactaatc tgatgaagaa atttatactc tacacttttt
      721 ttgtattggc aggttacggt cttcaggcgc agaatggaaa ccggctggag gcattgaaga
      781 tcgcctacat caccaaaaaa atggacctta gtccggagga agcacaacgt ttctggccca
      841 tctataatca gtatgctgca gaactcaaac aggcccgcca ggaggcactg aggaataatc
      901 aaaccgagat cgatctggac gaaacccagt tgagaatcag aaagaaatac aatagccagt
      961 ttagccaggt catttctccc caaaaagtaa atattttctt caaatccgaa aaagagtttg
     1021 gtgtatatgt tcagaaagaa atggaacgac gcgaactccg ggcccagaaa aggaacggaa
     1081 acagaccata gaccctgaga tttcccgaat ttaccgattt atttcagcta aaaaaacttc
     1141 aatgccgata tttgaaactg gtgtttttca taggttagca acggccggct ctttcaagag
     1201 cgggcctttt ttatagggcc gttgctctgc tttcatccgt tccgtccgga tcctgcagac
     1261 cgctttcgtt taacgcttta ataacatggc aggccagctt gcataaaaag aattcccggt
     1321 ttttctgttt tcatttaatt tcatccgtaa aatccggcgt tgaaaattgt ttccttacga
     1381 tgaggccgga cccaacccaa tacgcgatga agaaatctta cgtttttatt cttgttcttt
     1441 ttacgctccc gggccttctt atggctcagg acaccgcaca gcaaattatt ccgggcagaa
     1501 caaatcagga gagccagctg aacaaaccct atgtgatcct gatctccgcg gatggattcc
     1561 gttacgatct ggcagataaa taccaggcaa aaaacctgtt gaagttccgg aaacagggcg
     1621 tcaatgccga ttatatgcag tcttccttcc cctcgctcac tttcccgaat cattatacta
     1681 ttgtgaccgg gctttacccg gcccatcacg gactggtgga caattccttt tatgatcagt
     1741 ccaagaatga atattattca ttgggaaata aagcagcggt ccgcgacagt tcctggtacg
     1801 gcggaacacc gctctgggtg ctggcggaaa aagcgggcat gctaacggcc agtttttact
     1861 gggtgggatc tgaagcggct atccagggtg tgagacccac ttattattat aatttcaaca
     1921 acctgatccc aatggatacc cggatcgggg aagtccgcag ctggctcgaa ctgcccgaag
     1981 aaaaaaggcc ccacctcatt ttcttttata tccccgatgt ggatcaccag gaacatttac
     2041 ataccgcaga ttcaaaagaa acggaaaaag cggttcattt tgtggatgaa agcattggga
     2101 aactggtccg gtctgtggat tcgctgcatt tacccgtaga ctttattttt gtatccgatc
     2161 acggcatgac gaccgtcgat acggcccatg gcattgaatt gcccgcagcc ctggatacaa
     2221 atcagttccg gatcgttaat tccctaaccc tcgttcacct gtacgcgtat aatcaaaagg
     2281 atgtcctgac cacagagata gcccttaaat cccaggcaac agattacaat gtttacctcc
     2341 cggaggaaat accggcggaa tggcattaca gcaaaacgga tgactggtac aaccggatcg
     2401 gtgatattgt tctggtgagc catcccccaa aagtttttaa ttttaaaaag cgccgcatta
     2461 atcccggtaa ccatggatat gatcccagcc tgactgaaat gcacgcctgc ttttatgcct
     2521 ggggcccgga tttcaaaaaa cattaccaga ttcccggatt cgaaaacgta aatgtatttc
     2581 cattgatcgc aaaaatactc aggctccggt attcagatcc gattgacggc cacttacggg
     2641 tgctgaagaa gacgctgaga tgagcaggat taccggtttg cctgattttc aaattcaaac
     2701 accggtgtct taatatcgtg ataaaacaga aaagtccatt tctcctcccg gccctggtcc
     2761 caccactgct ggcgaagcag catgcacttt ttcccgtcat aatcatactt ggctacaaac
     2821 cggctcttca tctggtttaa ttgcggagca acatccccgc caaaaaacaa atgctgtcca
     2881 tcttcttcca ggtgaaacac ctgatgaaaa ggacagtgac caccggtgag ttcaaaccgg
     2941 atataatcat cgattttccc gttaccttcc agttttacga ggttcgaaaa cgaattcagg
     3001 aaagcaaata attccggctc ataactggga ggaccctttt caatagcata ctccagctcc
     3061 cgctgctgta tataatactt cgcctgtggg aatgcggcca cccggcctcc gtccggcgcc
     3121 tttttcccca tgccgcccac atgatcctta tgtaaatgac tcatcaaaac ttttgtaacg
     3181 gaatccggat cgatgccggc agttttcatc agctgatgta attggagctc accatttttt
     3241 tcaaagccca gcccggtatc cagcaacaaa acgtcattgc gggttatgac cacaaagggc
     3301 tgaatctcga ccagcaggct tcccggtgcc ctcttttgca gatcatcctg acctggttcg
     3361 aacggaataa attcctttgt ttttcctacg gtgaacgcgc cttctgagag cgggatgatt
     3421 cgcatggcgc aaagttaggg gatgccggcg ggccagccat ttccctgttt ccaaaccgga
     3481 gaacaattga ttttcctcag cgcagcacca tttgccggtc cgagtcaagc cggatcagaa
     3541 taaaaagcat cacgctgaag gttagcaggg aagttcctcc ataactgaca aaaggaagcg
     3601 ggatgccgat caccggggca aggccgatgg tcatgcagat gttgatcgca atgtgaaaaa
     3661 agaaaacgga tgccaccccg tatgcatacg cgcggctgaa tacgcttcgc tgtcgctccg
     3721 cgatggttac aatgcgcatc agcaggatac cgtatatgca cagaaaaata acactgccgg
     3781 taaatccgaa gctttctccg atcgtacaga atataaaatc cgttcgctgt tccggaacga
     3841 a
//
LOCUS       DASYCU010000505         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4022637, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000505 DASYCU010000000
VERSION     DASYCU010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4022637"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VGM24_10140"
     CDS             <1..658
                     /locus_tag="VGM24_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEY4155779.1"
                     /translation="DFAERLRWFLANQPQMAALVSRWGEKGKDEKHLLSLLRGHRMKR
                     ILYRMLDENEFLSDHGIRALSKYHEANPYVMQIDGTQLSIKYTPGESDIPIFGGNSNW
                     RGPVWMPANYLIIESLKRFHDYYGDDFKVEYPTHSGNYFSLKEISEKLAERVVSLFTR
                     DQNGNRPIYGNNPLLQSDPNFKDYVLFFEYFHGDTGLGLGASHQTGWTALVSRLLMDK
                     "
     gene            698..>1611
                     /gene="obgE"
                     /locus_tag="VGM24_10145"
     CDS             698..>1611
                     /gene="obgE"
                     /locus_tag="VGM24_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY4155780.1"
                     /translation="MEKSNFVDQIRVFCRSGHGGAGIKHFLRTKYNALAGPDGGDGGR
                     GGHIILRGNKNTWTLLHLRYFKNVLAEDGEAGSGNKCTGRFGKDVYIDVPLGTIARDE
                     TTGLKEAEILEDGQEIIWLKGGRGGLGNAQFATPTNQAPEHAQPGEAGTEGWKVLELK
                     VLADVGLVGFPNAGKSTLLSVVTAAKPKIADYAFTTLSPQLGMVEYRDGKSFCIADLP
                     GIIEGAAEGKGLGHRFLRHIERNVALLFLIPADSPDHRKEYEILLNELRQFNPELLHK
                     RFVIAISKSDLLDEELMEAIEKELPADIP"
BASE COUNT          415 a          395 c          434 g          367 t
ORIGIN      
        1 tgattttgcc gaacggctgc gttggttctt agcaaatcag ccccagatgg cggccctggt
       61 ttcccgctgg ggcgaaaagg gaaaggatga aaaacatctc ttgtccctcc tgagggggca
      121 ccgcatgaag aggattctct atcgcatgct ggatgaaaac gagtttctca gcgaccacgg
      181 catccgggcc ctttccaaat accatgaagc gaatccttac gtcatgcaga tagatggcac
      241 ccagctctcc attaagtata ctcccggcga atccgatatt ccgatattcg gcggcaattc
      301 caattggcgc ggaccggtct ggatgccagc caattacctg atcattgaat ccctcaaacg
      361 gttccatgat tattatggag atgatttcaa agtggagtac ccgacccatt ccggaaacta
      421 tttttcactg aaagaaatct ctgaaaaact ggccgaacgg gttgtttccc tgtttacccg
      481 ggatcaaaat ggaaaccggc cgatatacgg caataatccc ctgcttcagt cggatcccaa
      541 tttcaaagat tatgtcctct ttttcgaata tttccatggg gataccggcc tcggcctggg
      601 cgcatcacat caaacgggtt ggaccgccct ggtaagccgt ctgctcatgg acaagtaagc
      661 cgcttaatca gttatctttg ctgcccgaat ttaaaaagtg gaaaaaagca attttgtaga
      721 tcagatcagg gttttttgtc gcagcggaca cggaggtgcc ggcattaaac atttcttacg
      781 caccaaatac aatgcattgg caggtccgga tggcggcgac ggaggacggg gcggccacat
      841 cattctccgg ggaaataaaa acacatggac cctccttcac ctgcgttatt ttaaaaatgt
      901 gctggctgaa gacggggaag ctggcagtgg aaataaatgt accggccgct tcgggaaaga
      961 cgtttacatc gatgtgcccc tgggcacgat tgcccgggat gaaaccaccg gtttaaaaga
     1021 ggcggagata cttgaagacg ggcaggaaat catctggtta aaagggggtc ggggcggttt
     1081 gggaaatgcg cagtttgcca ccccaaccaa ccaggcgccg gaacacgccc agcccggaga
     1141 agccggcacg gaaggctgga aagtgcttga gctcaaagtg ctggccgatg tgggcctggt
     1201 gggatttccg aatgccggca agtcgaccct gctttcggtc gtcactgcag ccaagcccaa
     1261 aatagcagat tatgctttta ccactttaag cccccagctg ggcatggtgg aataccggga
     1321 cggcaagagt ttttgcattg cagacctgcc cgggatcatc gagggagccg cagagggcaa
     1381 gggcctggga catcgctttt taagacatat tgaacggaac gtggctttgt tgttcctgat
     1441 tcctgccgat agcccggatc accggaagga atatgaaata ttgctgaatg aattgcggca
     1501 attcaatccg gaactgctgc acaagcgttt tgtgattgcc atcagcaaaa gcgacctgct
     1561 ggatgaagaa ttaatggaag ccattgaaaa ggaattgccg gctgatattc c
//
LOCUS       DASYCU010000506         9864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3444822, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000506 DASYCU010000000
VERSION     DASYCU010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3444822"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGM24_10150"
     CDS             complement(<1..206)
                     /locus_tag="VGM24_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4155781.1"
                     /translation="MDKNAGWQWEINANQGWFRINFKEILQYKELIIRFVRRDIIASY
                     QQTILGPIWVFLQPLFTTFVYFII"
     gene            complement(295..672)
                     /locus_tag="VGM24_10155"
     CDS             complement(295..672)
                     /locus_tag="VGM24_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY4155782.1"
                     /translation="MLKIMIVEDDLDLLHGLRFLLQAYNCKICPISDADEILKTAGLL
                     QPGLILLDIKLDHGNGLDICRELKASYLKDTPVYIMSGLPDAEKKSYEAGANYFLAKP
                     FDSEVLLSKIKALEASSSEKFSF"
     gene            complement(829..1032)
                     /locus_tag="VGM24_10160"
     CDS             complement(829..1032)
                     /locus_tag="VGM24_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155783.1"
                     /translation="MENGSSAKRKEDEPGQKEKDQSNKRDSGIKAENGHLGSSNAFEK
                     TENPESADPDGITDEKIEEWLDE"
     gene            complement(1042..1365)
                     /locus_tag="VGM24_10165"
     CDS             complement(1042..1365)
                     /locus_tag="VGM24_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155784.1"
                     /translation="MRIHHAAGFIAMIFGCIFLSLAFRSYWNSPVKGSIVPASAGSRA
                     WVFSKTDTLSAPILQGNFMIEHVKPGNYTLMIEGKPPYRNAVKQGVIVVDGQLTDAGV
                     IQMNQ"
     gene            1443..2726
                     /locus_tag="VGM24_10170"
     CDS             1443..2726
                     /locus_tag="VGM24_10170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4155785.1"
                     /translation="MDKQEVLIIGAGISGLVMARMLCRAGLKVAVIEARDRIGGRILS
                     IRDRGVTLEAGPEFIHGHLKETVSLLNEYRIPFKETAGKMYYARSGRLELYSEENGDW
                     DLLIKKMKGLKKDLPFTEFLMVNFNAEKFRKLRESAIRFAEGFDLADVHKVSSISLIR
                     EWEQEESVQYRIPGGYGQLTEAISREFILSGGKIYLNCPVRELQWVRGDVLITASENQ
                     KFRAGRVIVTLPVGVLNHAGYADNAVRFSPELADKKIALQQIGFGTVIKMVLLWDTTF
                     WKRFTPDLGFIFSEEDIPTWWTQSPTDSNVLTGWVGGKKAETLSGLPEPELLKIALQN
                     LSAIFGLTQAELNAQLVSAKCFNWKKEIFTGGAYSYTLAGYEKARVTWKLPVEDTLYF
                     AGEACYDGPHGGTVEAAIVSGLETARELLKQYRAT"
     gene            2829..3107
                     /locus_tag="VGM24_10175"
     CDS             2829..3107
                     /locus_tag="VGM24_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155786.1"
                     /translation="MYSLVDDRISEKGFVSTIHVLRNGEIIYSVQEFMKIENRFSWVD
                     KKSLVRKILDLQKKTDDRKRSYIVIYEEGNMIREFLNVDQSFRPLPFC"
     gene            3241..3462
                     /locus_tag="VGM24_10180"
     CDS             3241..3462
                     /locus_tag="VGM24_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155787.1"
                     /translation="MKWIIFSGLLLLGSAAISCNQNSTGSATGDTTVKRVVPPASDNS
                     QATNPSLADTSYPKKDSMVTPGDSARSKK"
     gene            complement(3584..4804)
                     /locus_tag="VGM24_10185"
     CDS             complement(3584..4804)
                     /locus_tag="VGM24_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEY4155788.1"
                     /translation="MKVQDNHPPTQHAGKAFSAEIRSELDHILDYWKRFSVDCERGGF
                     VGKADNANRPVPGAAKAAVLNSRILWTFSAAWPVTKDPEQLALATTAYDYIRDHFSDP
                     VHGGLFWSVDAGGNPQDTRKQVYAQSFGIYGMSEYYRATGNEEALALAIEWYRLIEQY
                     GLDKQHGGYIDAFARDWSFLEDKRLSPKDDNAVKTTNTHLHLIEAYANLYEVWPDPLL
                     RVRIRELLALFQEKILNKNNYHLELFFDAEWNRKPGAVSYGHDIEAGWLLQSCAESIS
                     DEAWITRTKANALRISDAAMEGLDEDGGLWNEPDTEKSLIIKEKHWWPQAEMLIGLCN
                     AWQISATDRYQQAMLKNWQFIKKYIRDKENGEWFWGINEDHSVMPGQDKIGPWKCPYH
                     NGRACLQLIKRLTP"
     gene            complement(4817..6022)
                     /locus_tag="VGM24_10190"
     CDS             complement(4817..6022)
                     /locus_tag="VGM24_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HEY4155789.1"
                     /translation="MKGVFAERLNKLKKNHREILRRKNRVVELNSSGVFERYLFPVLT
                     REHIPLNWRYDLNEKTNPFLMERFGINTVMNAGAIKWNNKYLLMARVEGVDRKSFFAI
                     AESPNGIDQFTFWEKPVTIPILDRLETNFYDIRLTPHEDGWIYGLFCTEKRNPDAPEE
                     DQSAAIAQCAIARTRDLVNWERLPNLVTPSLQQRNVVLHPEFEKGQYAFYTRPQDSFI
                     EAGRGGGIGFGLSPSIEQAHIREEMILDKKIYHTVYELKNGQGPSPIKTSKGWLHLAH
                     GVRNTAAGLRYVLYLFLTDLDDLTKVLHKPAGYFLAPEGEERIGDVSNVVFCNGWIED
                     EDGTVFIYYASSDTRMHVAVTSVERLLDYTLNTAAEGYTSAQSVQQINKLIEKNHQLL
                     HIEKVKEKV"
     gene            complement(6083..6862)
                     /locus_tag="VGM24_10195"
     CDS             complement(6083..6862)
                     /locus_tag="VGM24_10195"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY4155790.1"
                     /translation="MIKCISRICGFLLLQMICISAFAQKDIYEIRTYLLKSENQERST
                     DQYLKNAYLPALHRLGIHHIGVFKPIANDTASIKKIYVLVPYSSLDEWRKTKISLQND
                     HSYTEAAKPFAEADTANYPFVRMESTLLEAFPKHTRLTVPEIKVNPETVYELRSYESP
                     TSRLHNKKVTMFNDGGETGLFKRLGFNAVFYAEVLSGCHMPNLMYMIVFENSQAREEH
                     WKTFGNDPEWKKISGDPLYENRYSVSHIDSILMHRTDYSDL"
     gene            complement(6859..7977)
                     /locus_tag="VGM24_10200"
     CDS             complement(6859..7977)
                     /locus_tag="VGM24_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY4155791.1"
                     /translation="MNFKPPLLLIFLLSFFRLNAQETADPHATPATRALFRNLQRLSG
                     KKILFGHQDDPAYGVGWKYEKGRSDIRGVTGEFPGVYGFDLGRIELDQDHNLDGVPFE
                     KTRQYIMDAYARGGVITLSWHLNNPLTGSTAWDNKPGAVASILPGGEKNELYTKWLDR
                     VAGFLQKLKGAKREPIPVILRLFHELNGGWFWWGKDQCSPEEIKSLWHFTIHYLTHQK
                     KIHQLLFAYNTDRFNSIPEYLERYPGDAWIDILGFDIYQANNIKENSAFAAFFEKDLD
                     LLDRVAAEHGKIPALTEFGYNQVPDSNWWTGTFLPVIEKHRVVYALAWRNAGAHENGS
                     QEFYVPYPGQASAADFKKMFRSGKIIFETSLQTEKIYK"
     gene            8289..9164
                     /locus_tag="VGM24_10205"
     CDS             8289..9164
                     /locus_tag="VGM24_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY4155792.1"
                     /translation="MKKNLLREITPLTQSDCFTLFSRVKSAFDFPLHYHEEFELNFIQ
                     NAKGAKRVVGDHMEEIDDLELVLVGPNLQHAWFTHRCKSPEIREITIQFHKDLFDERL
                     LRRNQLSFIKSMFDKSSRGILFSRDTIQALAPRIAELNQKQGFDSVLELMSILHDLSI
                     SRNMRILSDLSFTTEQFTYNSRRVERTFEYLNKNFDKNITLAEVSKLANMSDVSFSRF
                     FRQRTGNTFIDSLTEIRLGHASRLLIESTNSIAEVAYHCGFNNISNFNRIFKKKKGCT
                     PREFRDSFSGTRIFI"
     gene            complement(9235..>9864)
                     /locus_tag="VGM24_10210"
     CDS             complement(9235..>9864)
                     /locus_tag="VGM24_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4155793.1"
                     /translation="IVEDNPSLRNSLVNLFDKTEGIRCVAALNNLMNAVSDIGHASPD
                     IVLMDIGLPNISGIEGVRTLKSNFPGIQIMMFTVFDDEERIFDAIRMGASGYLLKKTP
                     PEEIVLAIKELDQGGAPMSASIARKVIQSFQSPGPGTEDFNLTVREKEILYSLVDGLS
                     YKKIAEKYCISISTIRTHICNIYTKLHVHSKSAAVAKILGRSGSSGPID"
BASE COUNT         2446 a         2290 c         2362 g         2766 t
ORIGIN      
        1 aaaataatga aataaacaaa tgtggtaaat aagggttgta gaaaaaccca gatcgggccc
       61 agaatagttt gctgataact ggcgataatg tcccttcgaa caaaccggat aataagttct
      121 ttatactgga gaatttcttt aaagttgatc cggaaccatc cctggtttgc attaatttcc
      181 cattgccatc cggcattttt gtccatgtgg tccaaattca gtcagttacc ggaaaagtaa
      241 gcattttcgg ctaataatga aaaggaagct gctggtgaaa taacgcaata aaatttagaa
      301 actgaatttt tcggaggagg acgcttcgag ggcttttatc tttgaaagca gcacttctga
      361 atcaaaaggt ttggccaaaa agtaattggc gccggcttca taggatttct tttctgcatc
      421 cggcaatccg gacatgatat ataccggtgt atctttaaga tagctggctt tcagctcccg
      481 gcagatatcc agcccatttc catgatccag tttaatatca agcaaaatca gaccgggttg
      541 caggagaccg gctgttttta gaatttcatc cgcatcagag atcgggcata ttttgcagtt
      601 ataggcctga agtaaaaacc ggagcccgtg aagcaggtcc aggtcatctt caacaatcat
      661 tattttaagc atgttatgaa tatatgaatg tttttagtac ctatttaatg ccagaaaatc
      721 tcctgtttaa aatagaaacg ttatttcttt gttgggcaaa gaattcccca caaattacag
      781 tagaaacttc gtaatatccc gggatctttt cccattaatg gaaaaacctc attcatccag
      841 ccattcttct attttttcgt ctgtgatccc atccggatcg gccgattccg ggttctccgt
      901 tttctcaaat gcattggagg aacccaggtg cccgttctcc gcttttattc cggagtcccg
      961 tttattcgac tgatcttttt ctttttgccc aggttcgtcc tcttttcgtt tggcggaaga
     1021 cccattttcc ataataaaat tttattgatt catttgaatg acaccggcgt ccgtgagctg
     1081 accgtccacc acaatcacgc cctgcttgac tgcattccgg tatggcggct ttccttcgat
     1141 catcagcgta taatttcccg gcttgacgtg ctcgatcata aaatttccct gcaaaatagg
     1201 agcgcttagc gtatctgttt tggaaaagac ccaggcgcgg ctacctgcgc tggccgggac
     1261 aatagaacct ttgacggggc tattccagta agaccggaag gccagggata ggaagatgca
     1321 tccgaagatc atcgctataa atcccgccgc gtgatgaatg cgcatatgag ttcaattgat
     1381 ttatcagcgg tgcaattatt attccttacc ggcggggccg taattttgag gtgaacagga
     1441 aaatggataa acaggaggta ctgattatcg gagccggaat ttccgggctg gtcatggcac
     1501 gtatgctttg ccgcgccggc ttaaaagtag ccgtcatcga ggccagggac cgtattggcg
     1561 ggcggatctt aagtatccgc gaccggggag tcaccctgga agcagggccg gagttcatcc
     1621 acggacacct gaaagaaacg gtttcccttt taaatgaata ccggattccc tttaaagaaa
     1681 cggccggtaa aatgtattat gcgaggtccg gccgtttgga gctgtattcc gaagaaaatg
     1741 gggattggga cctcctgatt aaaaaaatga agggcttaaa aaaggacctg ccttttacgg
     1801 agttcctgat ggtgaacttt aatgctgaaa aattccggaa gctcagggaa tcggccatcc
     1861 ggtttgctga aggatttgat ctggcggatg tccataaggt gagcagtatc tcccttatcc
     1921 gggaatggga acaggaggaa tccgttcaat accggattcc cggcggatac gggcaactta
     1981 cggaggctat atcccgggaa tttattcttt ccggcgggaa gatctacctg aattgcccgg
     2041 taagggaatt gcagtgggta agaggggacg ttttgatcac ggcatccgaa aatcaaaaat
     2101 tcagggcagg aagagtaatt gtaaccctgc cggtcggcgt attaaatcat gccggatatg
     2161 cagataatgc ggtgcggttt tcccctgaat tagcggataa aaaaatcgcc ttgcagcaga
     2221 tcggattcgg taccgttatt aaaatggttc ttttatggga caccactttc tggaaacggt
     2281 ttaccccgga tctgggattt attttttcgg aagaggatat tcccacctgg tggactcaaa
     2341 gcccgacaga ttcgaatgtc ttaacaggct gggtcggcgg aaaaaaagcg gaaacgctct
     2401 ccggattgcc ggaaccggaa ttgctgaaga ttgccctcca aaacctgtct gccatctttg
     2461 gattgacaca agccgaattg aatgcacagt tagtgagcgc aaaatgtttc aattggaaaa
     2521 aggaaatttt tacgggaggt gcatacagtt atacgctggc gggatatgag aaggccagag
     2581 tcacctggaa acttcccgtg gaagacaccc tgtattttgc aggcgaagcc tgttatgatg
     2641 ggccgcacgg gggaacggtg gaagcggcca ttgtcagtgg attggaaacg gcccgcgaat
     2701 tattaaaaca ataccgggcc acataaagaa ttttcattgt ttgaatagcg ttctgttcta
     2761 aacttgtgga taagaatcag caataataag ttggatgtcc tgtaaattca ctttacaacc
     2821 ttgtttctat gtattcatta gtcgacgacc gtatttctga aaaaggattc gtaagtacaa
     2881 tacatgtact ccgtaacgga gagattattt attccgtgca ggagttcatg aaaattgaga
     2941 atcggttttc ctgggttgat aaaaaatccc tggtgagaaa gatcctggat cttcaaaaga
     3001 aaacagacga caggaaacgg tcttatattg tgatttatga agaaggaaac atgatccgtg
     3061 aattcctgaa tgtggatcag agtttccgtc cccttccttt ttgttaggat ctctcccggg
     3121 tggtatctct tgttccttcc cccgttgata tttatttcat actggccggc actcacggcc
     3181 cctgcctcta cgcggatgag gctggtacga tatttttata gatagtgaaa acacttgggt
     3241 atgaaatgga ttattttctc cggcctgctc cttttgggtt ccgcggccat ttcctgtaac
     3301 cagaattcca caggatcggc aacgggtgac acaacggtca aacgggtagt gcctccggct
     3361 tcagacaata gccaggcgac caacccttcc ctggctgata ccagctatcc gaaaaaagat
     3421 tctatggtca cgcccggaga ttccgccagg tcaaaaaaat aaaataaaat tttttaccat
     3481 gattatttcc ggagttcttt ttctgctggc atctctttgg atatgcattt atttgtaccg
     3541 tatgccgacc acccaaaaaa agcggccgta ctagcagatc gggttaaggg gttagccttt
     3601 ttatcagttg gaggcaggcc cggccattat ggtacggaca tttccacggc ccgatcttat
     3661 cctggcccgg cataaccgaa tggtcttcat ttattcccca gaaccattcc ccattttctt
     3721 tatcgcgtat gtattttttt atgaactgcc agttctttag catagcctgc tgataccggt
     3781 ctgtggcgga gatctgccag gcattgcaca ggccgatgag catttcagcc tgtggccacc
     3841 agtgtttttc ttttatgatc aggctttttt ctgtatccgg ttcattccaa agaccgccgt
     3901 cttcatcaag cccttccatg gcggcatctg agatccggag tgcgttcgct ttggtccggg
     3961 tgatccaggc ttcatccgaa atggattctg cacatgactg taatagccag cctgcttcga
     4021 tatcatggcc atacgatacg gcaccgggtt tccggttcca ttcggcatca aagaaaagct
     4081 ccaggtgata attgttttta ttcaggattt tttcctgaaa gagagcaagc agttcccgga
     4141 tgcggacccg gagaagcgga tcgggccaga cttcatacag gtttgcatac gcttctatga
     4201 gatgcaaatg ggtgttcgtt gttttaaccg cattatcatc ttttgggcta agccgtttgt
     4261 cttccaggaa cgaccagtca cgggcaaacg catcgatata tcccccgtgc tgtttatcca
     4321 ggccgtattg ctcgataagc ctgtaccatt cgatggcgag ggccagcgcc tcttcattgc
     4381 cggttgcccg gtaatattca ctcattccat atattccgaa agattgggcg tagacctgct
     4441 tgcgggtgtc ctggggattc ccgcccgcat caactgacca gaataatcct ccatggaccg
     4501 gatctgaaaa atgatcacgg atataatcat aggcagtggt ggccagggca agctgttccg
     4561 gatctttggt gaccggccag gctgccgaaa acgtccagag tatccgggaa ttaagcacag
     4621 cagcttttgc cgcgccggga accggtcggt tggcgttatc tgcttttcct acaaatccgc
     4681 ccctttcgca atcgaccgaa aaccgcttcc agtaatctag aatatgatcc agttcgctgc
     4741 ggatctctgc agaaaaagct tttcctgcat gctgggtggg gggatgattg tcctgaacct
     4801 tcattgaaat ggcgcgttaa actttctcct tcactttttc tatatggagc aactgatgat
     4861 ttttttcgat cagtttattg atctgctgaa cggattgggc agaagtgtac ccctctgccg
     4921 ctgtattcag ggtatagtcc aggagccgtt cgacagatgt gaccgcaacg tgcatgcggg
     4981 tatcggaaga agcataataa ataaataccg ttccatcttc gtcttcgatc catccattgc
     5041 aaaataccac gttcgataca tctccgatcc gttcttctcc ttccggcgcc agaaaatatc
     5101 cggcgggttt atgcagcacc tttgtcaggt cgtctaaatc agtcaggaag aggtataata
     5161 catatcgcag tcctgcggcc gtattgcgga caccatgcgc caggtgaagc catcctttgc
     5221 tggttttgat cggcgaaggg ccctgcccgt tttttaattc gtaaaccgtg tgataaattt
     5281 tcttatccag gatcatttct tcccggatat gagcctgttc aatggaagga gagaggccga
     5341 aacctattcc gccccctctg cctgcttcaa taaaactatc ctgcggccgg gtatagaagg
     5401 catattggcc tttttcaaat tccgggtgaa gcactacatt ccgttgctga agggaggggg
     5461 taaccaggtt tgggagcctt tcccagttta ccaggtcccg ggtgcgggca atggcgcatt
     5521 gggcgatagc ggctgactga tcttcttccg gagcatccgg gttccgtttt tccgtgcaga
     5581 acaggccgta gatccagcca tcttcgtgcg gcgttaagcg gatatcataa aaatttgttt
     5641 ccaaccggtc caggatgggg atcgtaacgg gcttctccca gaacgtaaac tggtcgatgc
     5701 cgttcggact ttcagcaatg gcaaaaaacg atttccggtc aaccccttcc acgcgggcca
     5761 tgagcagata tttgttgttc catttgatgg cgcctgcatt catcacggtg tttattccga
     5821 aacgttccat aaggaaggga ttggttttct cattcaggtc ataccgccag ttgagcggga
     5881 tgtgttcccg ggtcagcacc ggaaataagt accgttcaaa gacgccgctg ctgttaagtt
     5941 cgacaaccct gtttttcctc ctcaggattt cccggtgatt ttttttcagt ttgtttagtc
     6001 tttcggcaaa tacccccttc atgtactttg gtttatgctg tttgtctgtc gatgtccctt
     6061 ccatcccggg caggcatccg ttttataagt cggaataatc ggtccggtgc atcagtatgc
     6121 tgtcaatatg agaaacggaa taccggtttt cataaagcgg gtctccggaa atttttttcc
     6181 attccggatc attaccaaat gttttccagt gttcttcccg ggcctggctg ttttcaaaaa
     6241 caatcatgta catgagattc ggcatatgac agcccgacaa aacttccgca tagaagaccg
     6301 cgttaaagcc caggcgcttg aagagaccgg tttcgccgcc gtcgttgaac atggttactt
     6361 ttttattatg cagccggctg gtcgggcttt cataacttct cagttcatat acggtttccg
     6421 gattcacttt tatttcaggc acggtgagcc gcgtgtgttt cgggaatgcc tccagcaggg
     6481 tggattccat tcttacaaac ggataattgg ccgtatccgc ttccgcaaaa ggttttgccg
     6541 cctcggtata tgaatggtcg ttctggaggc tgatttttgt tttacgccat tcatccaggg
     6601 aggaatatgg tacgaggaca tagatctttt ttattgaagc cgtatcgttg gcgatgggtt
     6661 tgaacacccc gatgtgatgg attcccagcc ggtgcagagc cggcaggtag gcatttttga
     6721 ggtattgatc cgttgaccgt tcctgatttt ccgatttgag caggtaggtc cggatttcat
     6781 agatatcctt ttgggcaaaa gcggaaatgc agatcatttg caagaggaga aatccgcaga
     6841 tccgggaaat acatttaatc atttgtatat tttttctgtt tgtaaagagg tttcaaaaat
     6901 tattttcccg gaccggaaca tttttttaaa gtctgccgcg gaagcctgtc ccgggtatgg
     6961 tacataaaac tcctggctgc cattttcatg ggcgccggca ttccgccacg ccagggcata
     7021 gaccacccgg tgtttttcaa taaccgggag aaaggtcccg gtccaccaat tgctgtccgg
     7081 gacctgatta tatccaaatt ctgttaaagc gggaatcttt ccgtgctctg ctgccacccg
     7141 gtccagtaaa tccaggtcct tttcaaaaaa agcagcgaat gcactgtttt ccttaatgtt
     7201 atttgcctga taaatatcaa aaccgagaat atctatccat gcatccccgg gatacctttc
     7261 caggtattcg ggtatgctgt taaaccggtc tgtgttgtaa gcaaataaaa gctggtgtat
     7321 ttttttctga tgggtcaggt aatgaatggt aaaatgccaa aggcttttta tttcttccgg
     7381 gctgcattga tccttacccc accagaacca gcccccgttc agctcgtgga ataaacgcaa
     7441 aatgaccggg atcggttctc tttttgcgcc cttcaatttc tggagaaacc cagccacccg
     7501 gtccagccat tttgtataga gctcattttt ttcccctccg ggaaggatgg atgctacggc
     7561 tccgggctta ttatcccagg ccgtgctgcc cgtgagcggg ttgttcaaat gccagctcag
     7621 ggtaatcact ccaccgcggg cataagcgtc cattatatac tgcctggttt tttcaaatgg
     7681 aacgccatcc aggttgtgat cctggtcaag ctcgattctt ccgagatcaa atccgtatac
     7741 tcccggaaat tctcccgtga ctccgcggat atcgcttctt cctttttcat acttccagcc
     7801 aacgccgtat gccggatcgt cctgatggcc aaaaagaatt tttttcccgg ataaacgctg
     7861 aaggttccta aagagcgccc gggttgccgg tgtggcatgc ggatccgcgg tttcctgcgc
     7921 gttcagccgg aaaaaggaaa gcaggaaaat caggagaagc ggcggtttga aattcacgaa
     7981 ttaaatatcg gttttattgt tattagagaa tatcagatcg gttaacgggg ccttggaccc
     8041 aaaggatttg cataagactt accaaaacta ttcccaatct tatccttaaa taaatactaa
     8101 attagcaagt aattgtattt ttttactgat ccggcctcaa cccctcctcc taattgggtt
     8161 aaaagtatta gttataggcg aatattgtat ttttgatttt attccaaatg ttgatactat
     8221 ttttacctgt ttgaacgcag gtataatttt gtcagtaact caggcggtta acaggaacta
     8281 acttgcatat gaaaaagaac ctcttgcggg agattactcc gctcacccaa agcgattgct
     8341 ttaccttatt ttcccgtgtc aaatcggcct ttgattttcc gcttcattat catgaagaat
     8401 tcgaattgaa tttcattcag aatgccaagg gggcaaagcg ggtcgttgga gatcatatgg
     8461 aggaaattga cgacctggag ctggtgctcg ttggtccgaa cctgcagcat gcctggttta
     8521 cccatcgctg caagtcgccg gaaatccggg agatcacgat ccagtttcac aaagatcttt
     8581 tcgatgagcg gctgttgcgc cgaaatcagc taagctttat taaaagtatg ttcgataaaa
     8641 gttcgagagg gattcttttc tccagggaca cgatacaggc actcgcgccg cgaatcgcag
     8701 aactcaacca gaagcagggg tttgattcag tattggaact gatgtccatt ctgcatgatc
     8761 tatctatttc ccgtaacatg cgcattcttt ccgatctttc ttttaccacc gaacaattca
     8821 cgtataatag ccggcgggta gagcggacgt ttgaatacct gaataaaaat ttcgataaaa
     8881 atattacgct ggccgaagta tcgaagctgg ccaatatgtc ggatgtttcc ttcagccgtt
     8941 ttttccgcca gcgcactggc aatacattta tcgacagcct gacggagatc cggctggggc
     9001 atgccagccg gctgctgatc gaatcgacca actccatagc cgaagtggcc tatcattgcg
     9061 ggtttaacaa tatctccaat ttcaaccgca tttttaaaaa gaagaaagga tgcacgccca
     9121 gggagttccg ggatagtttt tcgggaacgc ggatattcat ctgaagaaaa agggcatctt
     9181 tcaggtgaaa gatgcccggg aaccgggttt aatgattaaa tggttatgga cgggttagtc
     9241 gatcgggccg gaagatccgg atcttcccag aattttagca acggccgcgg atttggaatg
     9301 aacatgcagt tttgtgtaaa tattgcagat atgggtgcgg atggtgctga tactgataca
     9361 atatttctct gcgatctttt tatagctgag gccatcgacc aaagaataca ggatttcctt
     9421 ttcccggacg gtaaggttaa aatcttccgt tccgggcccg ggtgactgaa aagactgaat
     9481 caccttccgg gcgatgctgg ctgacatcgg tgctcctccc tgatcaagtt ccttaatggc
     9541 cagtacaatt tcttccggtg gcgttttctt cagcaggtat ccggatgcgc ccatccggat
     9601 ggcatcaaag atccgctctt cgtcatcaaa aacggtgaac atcatgatct gtattccggg
     9661 gaagttggat ttcagggtcc tgaccccttc gattcccgaa atattcggca acccgatatc
     9721 catcagcacg atatccgggg aagcgtggcc gatatcactc accgcattca tcaggttatt
     9781 gagcgcggca acacagcgga tcccctcggt cttgtcgaac aggtttacca ggctgttgcg
     9841 tagtgaaggg ttatcctcca caat
//
LOCUS       DASYCU010000507         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_690687, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000507 DASYCU010000000
VERSION     DASYCU010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690687"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VGM24_10215"
     CDS             <1..1887
                     /locus_tag="VGM24_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY4155794.1"
                     /translation="MHYVSAEGLGKSFGVKPLFDNISFHIESGDKIALIAKNGTGKST
                     LLKILAGKETAENGKVWISKDITVSFFEQEPQFREELSLLDNIFHHNHPVTSAIKAFE
                     AASESGDTDALMESIDQMDHLGAWDFDARVKQIFSRLNIEHLIQPVKTLSGGQRKRVA
                     LARTLIDIGFGNPEVLLIMDEPTNHLDIEMVEWLENYINQQNITLLVVSHDRYFLDNV
                     CNEIWEMDRSALYEYKGNYENYLEKKAARLESDLSSIDKARNLYRKELEWMRKQPKAR
                     TTKSKSRQDAFYEVETKARQNLDEQQLKLEMKMSRLGGKVAELKKVNKSFGDKIILRG
                     FDYTFKRGDRIGVVGKNGTGKTTFLNMLQGLEPADSGKINIGETVVFGNYSQVGLVVK
                     EDKRVIEFVKDIAESFPLAGGGSLSAAQFLNLFLFPPDKQYTYISSLSGGEKKRLHLL
                     AILFRNPNFLILDEPTNDLDLPTLSVLENFLLDFQGCLVMVSHDRYFMDRMVEHLFVF
                     EGEGVIRDFPGNYGLYQIWLKTKETAPATAELRSPESRSAPEPLLKAEKQKLSYKEKR
                     EFELLEKEIGNLNREKENIVDKLREPSLAYEEIEKLSQRFTFISEDLQAKEFRWLELS
                     ELQD"
     gene            2011..>2267
                     /locus_tag="VGM24_10220"
     CDS             2011..>2267
                     /locus_tag="VGM24_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEY4155795.1"
                     /translation="MQAIIGVFFHFIGGFASGSFYMPYKGVKGWAWENYWLVGGLFSW
                     LIVPPLAAWLTIPHFTEIISAISADVFGYTFLFGLLWGIGGL"
BASE COUNT          628 a          537 c          581 g          521 t
ORIGIN      
        1 atgcattatg tttctgctga gggcctggga aagtcgttcg gggttaagcc actttttgac
       61 aatatttcct ttcatattga atcgggtgat aaaattgcgc tgatcgccaa aaacgggacg
      121 gggaagtcca ccctgttgaa gatcctggcc ggaaaagaga cggcggagaa cggaaaggtc
      181 tggatctcca aagacattac cgtcagtttc ttcgagcagg agcctcaatt ccgggaagaa
      241 ctttccttgc tggataatat ttttcaccat aatcatccgg taacctccgc gatcaaagcc
      301 tttgaagccg ccagcgaaag cggggatacg gacgcgctga tggaaagcat cgatcagatg
      361 gaccatctcg gcgcctggga ttttgatgcc agggtgaagc agatcttcag ccgtctgaat
      421 atcgaacacc tgatccagcc cgtcaaaacc ctgagcggtg gccagcgcaa gcgggttgcc
      481 ctggccagaa cgctgatcga tatcggcttc ggaaatcccg aggtattgct gatcatggat
      541 gaaccgacca accacctgga catcgaaatg gtggaatggc tcgaaaatta tatcaaccag
      601 caaaacatca cgttgctggt cgtttcccat gaccgctact tcctggataa tgtatgtaat
      661 gagatctggg aaatggaccg gagcgcacta tacgagtaca aggggaatta tgaaaattac
      721 ctcgagaaga aggccgcccg gctggaaagt gatctgtcca gcatcgataa agccagaaat
      781 ctctaccgca aagaactgga atggatgcgc aaacaaccca aggcgcgtac caccaagtca
      841 aagagccggc aggatgcttt ttacgaagtg gaaacaaaag ccagacagaa cctggatgaa
      901 cagcagctca aactcgaaat gaaaatgagc cgtctcggcg gcaaagtggc ggaattaaaa
      961 aaagtgaata agagttttgg cgataagatc attctgcgtg gattcgacta tacgttcaaa
     1021 cggggagacc ggatcggggt ggtgggaaag aacggcacag gcaaaaccac tttcctgaat
     1081 atgctgcagg gactggagcc tgcggatagc ggaaaaataa atatcggaga aacggtggtt
     1141 tttggaaatt attcccaggt ggggctggtc gttaaagaag ataaacgcgt aatcgaattt
     1201 gtaaaagaca tcgcagaaag tttccccctg gccggaggcg gctcgctcag cgctgcccag
     1261 tttctgaacc tctttctttt tccgccggat aaacaataca cctatatctc cagtctcagc
     1321 ggaggggaaa agaaaaggct tcatttactg gccatccttt tccgcaaccc gaacttcctc
     1381 atcctcgatg agcccacgaa cgacctggat cttccaaccc tgtcggtgct agaaaatttc
     1441 ctgctcgatt tccagggctg cctggtgatg gtttcccacg accggtattt catggaccgg
     1501 atggtggaac atttgtttgt atttgaagga gagggcgtga tccgggattt ccccggaaat
     1561 tatggattgt accagatctg gctgaaaaca aaagaaacag ctcccgcaac agcggaactc
     1621 cggagtccgg aatcccgttc cgccccggaa cccctgctta aggcagaaaa acaaaagctc
     1681 tcctataagg aaaaacggga attcgagttg ctggaaaaag agatcggaaa cctgaaccgg
     1741 gaaaaggaaa atatcgtgga caagctccgg gaaccttccc tggcatatga agagatcgaa
     1801 aaactgtcgc agcgctttac ttttatttcc gaagaccttc aggcgaaaga attccgctgg
     1861 ctggaactga gcgaacttca ggactgacgg gaacacggca taacccggca ggatccccac
     1921 ctccatttac atgacagtac atgacagctg tacttcattc attccctatt tttatgatcc
     1981 gacaaacaat acccggttaa taaaacgaat atgcaggcta ttattggagt tttttttcat
     2041 tttatcggag gatttgcatc cggcagtttt tacatgccct ataagggggt gaaaggatgg
     2101 gcctgggaaa attactggct ggtcggagga ttgttttcct ggctcattgt tcctccgctg
     2161 gcggcctggc tgaccatccc gcatttcacc gaaatcatct ccgctatttc cgccgacgta
     2221 ttcggatata cgtttttatt cggtctctta tggggaatcg gcggact
//
LOCUS       DASYCU010000508         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_110524, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000508 DASYCU010000000
VERSION     DASYCU010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110524"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VGM24_10225"
     CDS             complement(<1..756)
                     /locus_tag="VGM24_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEY4155796.1"
                     /translation="MPFKISRTNEQDLNLLQLTDTSTGIQVKILTDYGALLHEFSMPL
                     EKGRLQAVSGYKNLSDLRLNHSLFYRSAKLSPFPCRIFKGRYPFEGKSYEFKHKFTDG
                     SAIHGLLSDKPFALLQENAEDQSASVLLEYQYQAEDPGYPFPYRITVKYTLKKEGLLD
                     LQTTLTNLSSGRIPIADGWHPYFSLGGKVNDWILTVDAKEILAFDENLIPDGRQLPFD
                     FFSAPAKIDSFEFDHCFLPEFHPGKPVCVLFNPA"
     gene            782..1927
                     /gene="galK"
                     /locus_tag="VGM24_10230"
     CDS             782..1927
                     /gene="galK"
                     /locus_tag="VGM24_10230"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY4155797.1"
                     /translation="MEKQIRRQFQQYFGKDTLIAAAPGRVNLIGEHTDYNEGFVLPGA
                     IDKNIYIGIDSNPNRILNVYAEQYQEKFTFPIDDVHPVKGWPTYLLGMIYHLLPHRQL
                     SQGMDVVVSGDVPVGAGMSSSAALCSAFGLALNEIFRLGLSRMEIALAAQKTEHDFAN
                     LKCGIMDMFASLHGRTGQVMKLDCRNLEYEYIPFDFPDYRIVLVNSMVSHSLSTSAYN
                     ERRRQCEEGVSYLKRFYPEIRSLRDVQQEQLLPHRNEIDPVVFKRCLFVLEENQRLLE
                     GCDFLEAGRLDDFGKLMYASHEGLSRDYEVSCAESDFLVNAAKSFPAVKGARQMGGGF
                     GGCMIALVEKDQTDHFLKGIQEKYENRYGKTPDNYIMNIAEGARLLN"
     gene            complement(1930..>2963)
                     /locus_tag="VGM24_10235"
     CDS             complement(1930..>2963)
                     /locus_tag="VGM24_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155798.1"
                     /translation="VKYHFSIVPAIGYTLSTGFAGILASNVAFFTSDPGKTNESNIAV
                     QLVYSQYAQLTVPVLVNWWTRDNRYNIKADWRYYKYPQDTYGLGGHSSLDNDDEIDYS
                     QFRIHQTVLKRIMANGYAGLGYFLDYHWNVKETGLPDGEPSDAFKYGLAAKTVSSGPV
                     LDLLFDTRKNSINPEGGFYLDLLYRNNLKAFGSDINWQSLIVDMRKYFRLFPNSPNIL
                     AIWSYDWLTLSGKPPYLDLPATGWDTYNNTGRGYIQGRFRSENMIYLETEYRFIISRN
                     GLFGGVAFANMQSFSDYPETRFSKIWPAAGGGLRIKVNKHSNTNIAIDYGIGIDGSKG
                     FFVNLGEVF"
BASE COUNT          760 a          671 c          773 g          759 t
ORIGIN      
        1 tgccggattg aatagtacgc agaccggctt tccgggatga aattcgggca gaaaacagtg
       61 atcgaattca aaggaatcaa tttttgccgg tgcggagaag aaatcaaaag gcaattgccg
      121 gccgtcaggg atgaggtttt catcgaaagc caggatttct tttgcatcaa cggtcagtat
      181 ccagtcattt acttttcccc ccaggctgaa atacggatgc cagccatccg cgatgggtat
      241 ccggccggaa gataaattgg tcagggtggt ctgcaggtca agcagtccct ctttttttaa
      301 tgtatatttg acagttatcc ggtaagggaa agggtaaccc ggatcttctg cctggtattg
      361 gtattcgagg agaaccgaag ccgactgatc ctctgcgttt tcctgcagca aagcaaaggg
      421 cttatcggac agaagtccgt ggatcgcgga tccatcggtg aatttgtgtt tgaattcata
      481 ggattttcct tcaaagggat accggccttt gaaaatgcgg cagggaaaag gagacagctt
      541 ggcgctccgg tagaaaagcg aatgattcag ccggaggtcg gagagattct tgtaaccgga
      601 taccgcctgc aggcggcctt tctccagtgg cattgaaaat tcatggagca atgctccgta
      661 atccgttagt atctttacct ggataccggt tgacgtgtct gtcaattgca gcaggttcaa
      721 atcctgttca ttcgttctcg agatcttaaa tggcattaga taaaatttga taaattccct
      781 gatggaaaag cagattcgga ggcaatttca gcaatatttt ggaaaggaca cacttatcgc
      841 tgctgctccg ggaagagtta accttatcgg tgaacacacg gattacaatg aaggctttgt
      901 actcccgggg gcgattgaca aaaacatcta tatcggcatt gattccaacc cgaacaggat
      961 attgaacgta tatgcagagc aataccagga aaagttcacg ttcccgatag atgatgtgca
     1021 cccggtaaaa ggctggccga cctatctgct ggggatgatt tatcatctgc tgccccaccg
     1081 tcagctttcc cagggaatgg acgtggtggt atccggcgat gtgccggtag gggccggcat
     1141 gagttcatcc gcagcactgt gttctgcctt cgggcttgcc ctgaatgaaa ttttccgttt
     1201 gggtttatcc agaatggaga tcgcgctcgc agcacaaaag acagaacatg attttgccaa
     1261 tttgaaatgc gggatcatgg acatgtttgc gagtttgcac ggccggacgg gtcaggtgat
     1321 gaaactggat tgccggaacc tggaatacga atacattcca tttgattttc cggattaccg
     1381 gattgtgctg gtcaattcca tggtgagtca ttctctttct acttccgctt ataatgaacg
     1441 gcgccggcaa tgtgaagaag gcgtttccta tctgaaacgt ttttatccgg agatccgttc
     1501 cctgcgggat gtgcagcagg aacaattatt accccaccgg aacgaaatag atccggttgt
     1561 ttttaaacga tgccttttcg ttctggaaga aaaccagcgg ttgctggaag gttgcgattt
     1621 tctggaagcc ggccggctgg atgattttgg taaactgatg tatgcctcgc acgaaggatt
     1681 gagccgcgat tatgaagtga gttgcgccga atcggacttc ctggtgaatg cggccaaatc
     1741 attcccggca gtaaaagggg caaggcagat gggaggcggt tttggcggat gcatgatcgc
     1801 gctggtggaa aaagatcaga ccgatcattt cctaaaaggc attcaggaaa aatatgaaaa
     1861 ccggtatggc aaaacccccg ataattatat catgaatatt gcggaagggg cgcgcctcct
     1921 gaattgatat cagaatactt ctccgaggtt cacaaaaaat ccttttgacc cgtctatgcc
     1981 gattccgtaa tctatggcga tattggtatt ggaatgctta ttcactttaa tgcgaagccc
     2041 gcctccggcg gctggccaga tcttgctgaa ccgggtttcc ggataatcgg agaaactctg
     2101 catattggca aacgctactc ctccgaataa tccgttccgg ctgatgatga accggtattc
     2161 cgtttccagg tagatcatat tctccgaacg gaagcggccc tgaatatagc cccgccccgt
     2221 attgttatac gtatcccagc cggtggcagg tagatcaaga tagggtggtt ttcccgaaag
     2281 cgtgagccag tcgtaactcc agatagcaag aatattcgga ctattcggga acagccggaa
     2341 atatttccgc atgtccacga tcagggactg ccagttgata tcacttccga aggcctttaa
     2401 attgttgcgg tagagcaggt ccaaataaaa gcctccttcg gggttgattg aatttttccg
     2461 ggtatcgaat aacaggtcca gcaccggtcc ggaggaaacc gttttagcgg caaggccata
     2521 cttaaaggca tcggaaggct ccccgtccgg taatccggtt tctttcacgt tccagtgata
     2581 gtcgagaaaa tatccaagac cggcgtatcc gttcgccata atccttttta atacggtctg
     2641 gtggatacga aactgggaat aatcaatttc atcatcgtta tccagcgagc tatggcctcc
     2701 cagtccatac gtatcctgcg gatatttata ataccgccag tcggccttaa tgttgtagcg
     2761 gttgtcccgg gtccaccagt tcaccagcac cggaacggtg agttgcgcat attgcgaata
     2821 gaccagctgg acggcgatat tggactcatt tgtttttccc ggatcactgg taaaaaaagc
     2881 cacattggat gccaggatgc cggcaaaacc ggtgctcagg gtatagccga tcgccggaac
     2941 aatggagaaa tgatatttaa ccg
//
LOCUS       DASYCU010000509         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_254631, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000509 DASYCU010000000
VERSION     DASYCU010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254631"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VGM24_10240"
     CDS             <1..257
                     /locus_tag="VGM24_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155799.1"
                     /translation="PETPDPVLTSVDLTHPPPIKQPALLRPPATVRPVAVAVFTNPLI
                     VENQQPPKPVATVAELNVAAIGIRAEAGPPATGGPDAHAG"
     gene            329..628
                     /locus_tag="VGM24_10245"
     CDS             329..628
                     /locus_tag="VGM24_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY4155800.1"
                     /translation="MPEFPGGTEALRRYLSRNLRMPDTEKESGSQVRVIVKFVVGPDG
                     RVTGINTILSGGKAFDEEVQRVISRMPTWKPGIQNGRKVSVYFNLPVNFVIPAAY"
     gene            712..951
                     /locus_tag="VGM24_10250"
     CDS             712..951
                     /locus_tag="VGM24_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155801.1"
                     /translation="MTTEDRDPDMRSKGYMLRRSIMDYGMGVIIFCLGIFFLIAPRVG
                     VVFGIDDLFRYLFGILCILYGLFRVYRGYKKNYFN"
     gene            963..1883
                     /locus_tag="VGM24_10255"
     CDS             963..1883
                     /locus_tag="VGM24_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY4155802.1"
                     /translation="MNIWKRCMPFILLVFPELLISGCGHGNKIPIETATSGTIHISVD
                     ESFKPVIDSQLKVFESSYPDVKILVEYKPEAECLRDLARDSTRMIIITRGLSLKDEAF
                     YRDSLQKPAISGILAYDAVALIVNSRSRDTVMTMPEIRTLLEGNDKKNRRAVMDGVSA
                     TSTVRFAMDSILNGKPLGKNVEAARSSPQVIDYVASHEDAVGFIGVSWIGDQEDSVQL
                     SFLQKVKVVAVECASCPEKPYVQPFQANIVLKRYPMVRGLYYILKENYSGIGNNFVNF
                     LQNERGQLIFRRAYLVPARMTFNIRDVQLQ"
BASE COUNT          527 a          420 c          542 g          515 t
ORIGIN      
        1 tgccggagac gccggatccc gtcctgactt cagtcgacct gacccatccg ccgccaataa
       61 aacaacctgc gttgctccgg ccgccggcaa cggtaagacc tgtggcagtt gcggtgttta
      121 cgaatccact cattgtggaa aatcaacagc cgcccaaacc cgtggccacg gtggctgaac
      181 tcaacgtcgc ggcgatcgga atacgggcgg aagcgggacc gccggccacg ggaggacctg
      241 atgcgcatgc ggggtaacgg gaggagcagc ggcttatgga aaagattctg cggaatccaa
      301 accggaaatc ctggaccggg cggagttcat gcccgaattt cccgggggaa cggaagccct
      361 gaggcggtat ctgtccagaa acctgcgcat gccggatacg gaaaaggagt ccggcagtca
      421 ggttcgggtc attgttaagt tcgtggttgg accggacgga agggtaaccg gaatcaacac
      481 gattctatcg ggaggaaagg cctttgatga agaagttcaa agggttattt ccaggatgcc
      541 aacctggaaa ccgggcattc aaaacggcag aaaagtatct gtatatttta accttccggt
      601 aaattttgta attccggcag cgtattaatt tttgaatttt ttgacatggc ccaggccaga
      661 tatctacatg ttattgtgta tattgccttt tgcttttaaa tgtattgtta aatgaccacc
      721 gaagaccggg accccgatat gcgctctaaa gggtatatgc tcaggagatc tataatggat
      781 tatggaatgg gagtgatcat attttgtctc ggcatttttt tcctgatagc acctagggtt
      841 ggggtggtgt tcgggattga tgatttgttc cggtatctgt ttggcatact atgtattctt
      901 tatgggttat tccgggtgta ccgggggtat aagaaaaact attttaatta aaaattcccg
      961 gcatgaacat ttggaaaaga tgtatgcctt ttatactatt ggtattccct gaactcctta
     1021 tttccggatg tggtcatggg aataaaatac ctatagaaac cgcaacctcg ggcacgatcc
     1081 acatcagtgt ggatgagtct tttaaaccgg tgatcgattc ccagctcaaa gtgtttgaaa
     1141 gttcttatcc cgatgtaaaa atcctggtgg aatataagcc ggaagccgaa tgtttaaggg
     1201 atctggcaag agacagcacg cggatgatca ttattacccg cggcctgagc ctgaaggacg
     1261 aggcttttta ccgggactcc ctgcagaagc cggccatttc gggaatactg gcttatgatg
     1321 cagttgccct gattgtaaac agtcgttcgc gggacacggt gatgacgatg ccggaaatcc
     1381 ggaccctgct ggaagggaac gataagaaaa accgccgggc cgtgatggat ggggtttccg
     1441 ctacgagtac cgtgcgattt gcaatggatt ccatactgaa cggaaaacca ttgggaaaaa
     1501 atgtggaagc agccagatcc agcccccagg taattgatta tgtggcttca catgaagatg
     1561 cggtgggatt tatcggggtg agctggattg gagatcagga agattcggtg cagctttctt
     1621 ttctgcaaaa agtaaaagta gttgccgttg aatgcgcttc ctgtccggaa aaaccatatg
     1681 ttcagccatt tcaggctaat attgtgttga aaaggtaccc catggtacgt ggattgtatt
     1741 atatcctcaa ggagaactat agcggtattg gaaataattt tgtgaatttc ctgcagaacg
     1801 aacgcggaca attgatattc aggagggcct acctggtgcc tgcccggatg acatttaata
     1861 tccgggacgt tcagctgcag taaaaaatgt tttttgggat atatttttac acattaaact
     1921 gagatttaga atgagaaaac taagcatcag ccttatactg tcggttgcct gcatcggcgg
     1981 tacggttatg gcacaaacag taga
//
LOCUS       DASYCU010000510         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_255007, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000510 DASYCU010000000
VERSION     DASYCU010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255007"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3160
                     /locus_tag="VGM24_10260"
     CDS             <1..3160
                     /locus_tag="VGM24_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4155803.1"
                     /translation="FFIEGAKAQVAPLTGQVVDDSSSRGIPNVSVTVKGTKTGTSTDA
                     SGAFSIRAPKGSVLIFTSQGYTAREVPVNGNYLKVNLTSTAQNLANVVVIGYGQKDRR
                     LLTESIGTVSAKELQKLPVTSGDAAIQGRVSGVQITNSDGTPGAPVAVRIRGIGTVGN
                     TQPLFVVDGIPVGNNAGGVTNPLSTINPADIESISVLKDASSAAIYGMRAANGVVLIT
                     TKKGKMGAARVTLDAYYGIQKFPKKLKLNNTRQYVMLAQEALTNRNNQDGLSSGDPDY
                     RLLNPQLLASSPDNVLGINTDWQTPTINNNAPINNYNVGVSGGNENANYFFSVGHFGQ
                     QSITKVGGLERYSIRMNSDYKIGKRLKIGENLALSYENVRIGINGDGDGYLYSNDVSM
                     PPFFKIYDDQHTIPGNRYGYDGNYNVAGLSIGNQYGINQIVDRTSKTYRLLGGIYAEL
                     EIINGLKFRSAASVDFDYGRSLAWQPGFTAEEMGYGREVNNYSDNRSESYTQVFTNTL
                     NYEKILGEHTFNLLGGIEYQKLRGNNLGYTGNDFQSTSPAFYQSVSNGRGSPIGVYPD
                     ASSGLSNEAFSSYFGRLTYNYADKYLVTATVRRDGTSRFSEANRFGTFPAISAAWRIS
                     QEPFFKDNPVISDLKLRGSWGQLGNANTSNFAYVSRVSFTPQYPLDGVALQAPAPPSL
                     PNSDIGWETVESSDMGFDVSLFNKKVSLLATYYIRNTKNFLYSLPIPYTSGFGSTDVN
                     VGNVRNSGFEFDLSYNTTVAQKIHLSLSGNLTTIKNRLTALAPGVEEFNSGGGYRTGV
                     GYPIGYFYGYKALGIYQTPDQAAKALPDLDAGRAPSAGDIIFQDNNGPAKAGSPAGQQ
                     FSGQPDGQIGPEDRTYLGKTIPDFFYGLNINADYKNFDISIFFQGVSGVQVYNQNRQG
                     LEYLGGPAGNQLTTTLGRWTGPGTSNSMPRAVEGDPYDNGRFSSRWVEDAGFLRFKNI
                     QLGYTLPANLFGSTKVFKTARVYIAATNLFVITKYTGLDPEVMTYGRDSKYTNDNPLG
                     AGTDEGVIPQPKTIQAGLQVSF"
     gene            3185..>3694
                     /locus_tag="VGM24_10265"
     CDS             3185..>3694
                     /locus_tag="VGM24_10265"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155804.1"
                     /translation="MKKQYKLIGVLLFFTLGFLACKRQLDLQPLGELTDETFYQTEAD
                     FDAASLSPYATLLDFYWDQTGSPWYVPNLYPDDDVTVNSNQSNDVEDFNWLPSNGNFL
                     TVWQTCYKGIQRANVVLAQLPRAKGFQDESAKPRYEAEARFMRAYFNFILATYWGTPP
                     IADSSITSIE"
BASE COUNT          978 a          897 c          946 g          873 t
ORIGIN      
        1 ttttttcatt gaaggcgcga aggctcaggt tgctccgctc accgggcagg tggtggatga
       61 ctccagcagc cgcggcatcc ccaatgtgag cgttacggta aagggtacca aaacaggcac
      121 ttcaacggat gcgtcgggag cattctcgat cagggcgccg aaaggaagcg ttttgatttt
      181 taccagtcag ggatacacgg caagggaagt ccccgtgaat ggaaattatc ttaaagtgaa
      241 tctaacttcc actgcccaga acctggccaa tgtagtggtg atcgggtacg gacagaagga
      301 ccgtaggctt ctaacggaat ccatcggaac agtgtcggca aaagagttgc aaaagctccc
      361 ggtgaccagc ggggatgcag ccatacaggg acgggtttcc ggtgttcaga tcaccaattc
      421 agacggaact ccgggagctc cggtggcggt aaggatccgg ggcatcggta ccgtaggcaa
      481 tacccaaccg ctgttcgtgg tagacggaat tccggtaggc aacaatgccg gaggagtcac
      541 gaatccgctg tccacgataa atccggcgga tattgaaagc atctccgtgc tgaaagatgc
      601 ttcctctgcc gctatttacg ggatgcgcgc tgccaatggg gttgttctca tcaccactaa
      661 aaaagggaaa atgggtgcgg ccagggttac cctggatgcg tattatggta ttcagaaatt
      721 ccccaaaaaa ttaaagctca acaatacccg gcagtacgta atgcttgccc aggaagcact
      781 caccaaccgg aataatcagg acggcctttc ttccggcgat ccggattacc ggcttcttaa
      841 cccccagctg ctggcctctt ccccggataa tgtgttggga ataaataccg actggcaaac
      901 cccgaccatt aataacaatg ccccgatcaa taattacaac gtgggggtat ccggaggaaa
      961 cgagaatgcg aattactttt tttctgtcgg tcatttcgga cagcaatcca ttactaaagt
     1021 tggcggactg gaacggtata gtatccggat gaattccgat tacaaaattg gcaagcgttt
     1081 aaagattggt gaaaacctcg ctctttcgta tgaaaatgtc agaataggaa tcaacggaga
     1141 cggagacgga tacctttatt caaacgatgt cagcatgccg cctttcttca agatctacga
     1201 tgaccagcat accattccgg gtaaccggta tggatatgat ggaaattaca atgtagccgg
     1261 actgagtatc ggtaaccagt atgggatcaa ccagatcgtt gaccggacaa gcaaaacata
     1321 caggctgttg gggggaatat atgcagaact cgaaatcatc aatggcctca aattcagatc
     1381 tgcggcttct gtcgatttcg attatggaag aagccttgcc tggcagcccg gattcaccgc
     1441 agaagagatg ggatacggcc gggaagtgaa taattacagc gacaacagaa gtgaatccta
     1501 tacgcaggta tttaccaata cactgaacta tgaaaagatt ttaggcgagc atacttttaa
     1561 tttactgggc gggatagaat atcagaaatt aagaggtaac aacctgggtt ataccggtaa
     1621 tgatttccag agcacttcac ctgcctttta tcaaagcgtc agtaacggaa ggggcagccc
     1681 gatcggcgtg tatcccgatg cctcttccgg cttaagcaat gaagcttttt ccagttattt
     1741 cggcagactc acctacaact acgctgataa atatctggtc actgccacgg taaggagaga
     1801 cggcacgtcc agattttcgg aagcgaaccg gttcggaaca ttccccgcga tctctgccgc
     1861 ctggcggatc agccaggagc ccttcttcaa ggataatccg gtgatcagcg atttaaagct
     1921 ccgcggaagc tggggccaac tgggaaatgc aaacacctcc aactttgcct atgtttccag
     1981 ggtcagtttt acccctcagt atccgctgga cggagtagcg ctgcaggctc cggctcctcc
     2041 ctctttaccg aattccgata tcggatggga aacggtggaa tcatcggata tgggctttga
     2101 cgtctctttg tttaataaaa aagtcagcct cctggccact tattatatcc ggaatacaaa
     2161 aaactttctt tacagcctgc ccattcccta taccagcgga tttggttcca cggatgtcaa
     2221 tgtaggaaat gtaagaaaca gcgggtttga atttgatctc agctacaata ccacggttgc
     2281 acagaaaatt catctttccc tatccggcaa tctgacgacc attaaaaacc gcttgacggc
     2341 acttgctccg ggggtggagg aatttaattc cggcggcggt taccggacgg gagtgggata
     2401 tccgatcggt tatttttatg gatacaaggc gttaggtatt taccagaccc cggaccaggc
     2461 ggctaaagcc ctgcctgatc tggatgcggg ccgtgcaccc agtgcaggcg atatcatatt
     2521 tcaggataat aacggacctg ccaaagccgg atcgcccgcg ggtcagcaat tttcgggtca
     2581 gccggatggc cagatcggcc ccgaagacag gacatacctg ggaaaaacga ttcccgattt
     2641 cttttacggt ttgaatatta atgcggatta taaaaatttc gatatctcca ttttcttcca
     2701 gggagtaagc ggggtgcagg tgtataatca gaacaggcag ggtctggaat acctcggtgg
     2761 tccggccgga aaccagctga caacgaccct gggcagatgg accggtccgg gtaccagtaa
     2821 ttccatgccc cgcgccgtgg aaggtgatcc ctatgataat ggacggttct cgagccgctg
     2881 ggtggaagat gccggattcc tgaggttcaa gaacattcag ctcggatata ccctgccggc
     2941 aaacctgttt ggcagcacga aagtctttaa gactgcccgg gtatacattg cggctaccaa
     3001 tttatttgtg attaccaaat ataccggcct cgatccggag gtaatgacct acggaaggga
     3061 ttccaaatac accaatgata atccgctggg agccggcacc gatgaaggcg ttatccccca
     3121 gcccaaaacc attcaggccg gtttacaggt cagcttttaa atgtacagtc attaaaaaac
     3181 gaatatgaaa aagcaatata aattaatcgg ggtccttcta ttttttacgc ttggtttttt
     3241 agcctgtaag aggcaactgg atcttcagcc gctgggtgag ttaacggatg agacgttcta
     3301 tcagaccgag gcggacttcg atgccgcttc gctttcgccc tacgccacgc tgctggattt
     3361 ttactgggat caaacaggct ctccctggta cgtacccaat ttgtacccgg atgatgatgt
     3421 taccgtaaac agtaaccagt ctaatgatgt cgaggatttt aactggctgc cgtcgaacgg
     3481 gaactttcta acggtatggc aaacctgtta taaaggcatt cagcgggcga atgtagtgct
     3541 tgcccaattg cccagggcca aagggttcca ggatgaaagt gcgaagccac gctatgaggc
     3601 cgaagcccgg ttcatgaggg cctatttcaa ttttatcctc gccacctact ggggaacacc
     3661 tcccattgcg gattcatcga tcacttccat tgaa
//
LOCUS       DASYCU010000511         3576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4460461, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000511 DASYCU010000000
VERSION     DASYCU010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4460461"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGM24_10270"
     CDS             <1..205
                     /locus_tag="VGM24_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY4155805.1"
                     /translation="KSRSFHKIYLYIDKKSHMVSSGKILDKNGNRYIYTINNLNGKAS
                     ISDDSFVFDKSKHPGVEEVDLRQ"
     gene            complement(207..773)
                     /locus_tag="VGM24_10275"
     CDS             complement(207..773)
                     /locus_tag="VGM24_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY4155806.1"
                     /translation="MRKVVVAITGASGSVYASQLIEKLMKIKAQWDELSVIMTVNART
                     VWETELGNREYQHFPVTLYSTDDFSAPFASGSARYDTLIIVPCSMGTLGRIASGVSSD
                     LITRAADVILKERRRLICVVRDTPYNLIHIRNMETVTLAGGIICPASPSFYSKPKSLS
                     EVAATVVDRIIDLAGFDWDSFRWGEKNG"
     gene            complement(1066..1518)
                     /locus_tag="VGM24_10280"
     CDS             complement(1066..1518)
                     /locus_tag="VGM24_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155807.1"
                     /translation="MLLRNTILFLCLITALNSFAYPEKIPKTDSGKVIFSLHNASDRQ
                     SRIDSVYLIFDRYDRRGAGIIKQVYHPVNNEVEIKVFKGRYYVSIFCLGIYNKAGFDR
                     VITVRANRQRKIFLRLQASALFTPGMVKIPRENWDPSHLAILKMGSSR"
     gene            complement(1679..2935)
                     /locus_tag="VGM24_10285"
     CDS             complement(1679..2935)
                     /locus_tag="VGM24_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEY4155808.1"
                     /translation="MADIFEKFTEDQGPIGQHRKRAHGYFAFPKLEGEISSRMKFRGK
                     EMIVWSLNNYLGLANHPEVRKADAAAAAKYGLAAPMGARMMSGNSSEHEQLENELSAF
                     VKKEDTCLLNYGYQGMVSIVDVLCGRHDIIVYDAECHACIIDGLRLHAGHRFVYKHND
                     ISDFEKQIIRANALIEKQKAGGVLVITEGVFGMAGDQGKLKEITGLKKKYGFRLLLDD
                     AHGFGTLGKTGAGAGEEQGVQPDIDLYFSTFAKSMGSIGAFVSGEKAVVDYIRYNIRS
                     QIFAKSLPMPMVIGNLKRLELLRNNPSLKEKLWKNALKLQAGLKRKGFDIGKTDSVVT
                     PIYMTGDIPEATAMVMDLREKYGIFCSIVVYPVIPKGHIIYRLIPTSVHTDEDIQATL
                     DAFEATKKKLDAGDYKVEAIPDMAEA"
     gene            3035..>3576
                     /locus_tag="VGM24_10290"
     CDS             3035..>3576
                     /locus_tag="VGM24_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY4155809.1"
                     /translation="MIRKYLLWLIFIPTLCTNSGYGQSVSAPLARPKLVVGIVVDQMR
                     WDYLYRFYNRYQDGGFKRLINQGFSCENTLINYLPSYTAVGHTCVFTGSVPSISGISG
                     NDWTDQLSGARVYCTDDSTVQAVGAPGSPAGKMSPRNLLVSTVTDELRIATNFQSRVV
                     GVSLKDRASILPAGHAANAA"
BASE COUNT          934 a          841 c          796 g         1005 t
ORIGIN      
        1 caaatcgcgg agttttcaca agatctatct ctatattgac aagaaatccc atatggttag
       61 ctccggaaaa atattggata aaaacggtaa ccggtatatc tacaccatca ataacctgaa
      121 cgggaaagct tctataagcg atgacagctt tgttttcgat aagtcaaagc atccgggtgt
      181 ggaagaagta gatctgaggc agtgaactat ccgttctttt ctccccatcg aaaactgtcc
      241 cagtcaaatc cggcaagatc aattatccgg tcaactacgg ttgcagccac ttcggagagg
      301 gactttggtt tgctgtagaa ggagggagaa gccggacaaa taattccgcc ggcgagggtg
      361 accgtctcca tgttacggat atggatgaga ttgtagggcg tgtcgcggac gacgcaaatc
      421 agccttcgcc tttctttcag aataacatcc gctgcccgcg tgatcagatc agaagatact
      481 ccggaagcaa tcctgcccag ggttcccatc gagcagggta caatgatcag ggtgtcgtac
      541 cgggccgaac cggaggcaaa tggtgctgaa aagtcatctg ttgaatacag ggttacggga
      601 aaatgctggt attcccggtt tcccaattcg gtttcccaaa cggttcgggc attaaccgtc
      661 attattacag ataattcatc ccactgggcc tttatcttca ttaatttttc aatcaactga
      721 ctggcgtaaa ccgatccact ggctcctgta atggctacta cgacctttct catttttttt
      781 aaaaattccc gtgataacaa attaactgat tttcagttga tatgaatcaa ttttatagcc
      841 atctgaaaaa aaggatttgc attaggtatt ttttctttgt ctaaaacttt tatatttgac
      901 ttttcatagg ataaggttta atggttaatg gtatttgatt agtgcgggcc tcccttcaaa
      961 aaggaggtct tttttatgca acagcaggtt gcattactta aatgcggaag ctcagaccgt
     1021 atccattgga acaaataaac cattttttaa gcaaaaattg ccaacttaac gggaagaacc
     1081 catttttaga atggcgagat gggagggatc ccagttctcc cggggtattt ttaccattcc
     1141 gggtgtaaaa agtgctgagg cctgcaatcg cagaaatatt tttcgttgcc ggtttgccct
     1201 gaccgtgatg acccggtcga agccggcctt gttgtaaatg cccaaacaaa aaatgctcac
     1261 ataatacctt cctttaaata ccttgatctc cacctcatta tttaccggat ggtacacttg
     1321 tttaataatg ccggcgcccc gtctgtcata ccggtcaaaa atgaggtaca ccgaatcgat
     1381 gcggctctgc cggtcagatg cgttatgaag gctgaaaata accttacctg aatcggtttt
     1441 tggaattttt tccggataag cgaagctgtt cagcgccgtt atcaggcaca ggaataatat
     1501 ggtgtttcgt agcagcaatc cggttagttc aggtatcaac ggatgatcgg gaatgaacgg
     1561 actgattgca attcattaaa aagcgaaaaa agggatttct aatgaactca aggtctcggg
     1621 atataacgaa aaaagccgag ctttatgcag gcccggcttg aattagtttt ataaaccgct
     1681 aagcttcagc catatccggg atggcttcca ccttataatc tcctgcatcc agcttctttt
     1741 tggtggcttc aaaagcatcc agggtggcct ggatatcttc atcggtatga accgatgtgg
     1801 gaatcaaccg gtaaatgata tgacctttcg ggattacggg ataaacaacg atcgagcaga
     1861 aaattccata tttctcgcgc aggtccatga ccatggccgt cgcttccggg atatcgccgg
     1921 tcatataaat aggcgttact accgaatccg tttttccgat atcaaatcct tttctcttta
     1981 atccggcctg cagtttaagg gcgttcttcc agagcttttc tttaagggaa ggattgttgc
     2041 gaagcaactc caatcttttc agattgccga tgaccatagg catcggcaga ctcttggcaa
     2101 aaatctggct gcggatattg taccggatat aatcaacgac cgctttttca cccgaaacaa
     2161 acgcgccaat ggatcccatg gatttcgcaa aagtggaaaa atataaatcg atatccggct
     2221 gaacgccctg ttcttctccg gcaccggccc cggttttacc cagcgttcca aatccgtgtg
     2281 catcatccag gagtaaccgg aacccatact tcttttttaa accggtaatt tcttttaact
     2341 ttccctgatc cccggccatt ccaaaaacac cttctgttat aaccagtacg ccgccggcct
     2401 tttgtttttc aatcaatgca ttggcccgga ttatctgttt ttcgaaatcg gatatatcgt
     2461 tgtgcttata aacaaagcgg tgacccgcat gcaatcttaa tccatcgatg atgcaggcat
     2521 gacattcggc atcatacaca ataatatcat gccttccgca cagaacatcc acaatactga
     2581 ccatgccctg atatccgtaa ttgagcaggc aggtgtcttc tttttttaca aaggcggaga
     2641 gctcattttc cagttgttcg tgttcactgc tgttgccgct catcatacgc gcccccatcg
     2701 gagcggcaag cccgtacttg gcagcggccg ctgcgtctgc ctttcttact tccggatgat
     2761 tggccagtcc gagataatta ttcaggctcc atacaatcat ttccttgcca cggaatttca
     2821 tgcgactgct gatttccccc tccagtttgg ggaatgcaaa atacccgtgt gctcttttcc
     2881 tgtgctgccc gatcggtccc tgatcttctg taaatttttc aaagatatct gccattgtag
     2941 ctttaggttg aattaaaaaa ttgatgtaaa aataatgcat ttgatgaaca gggcaagccg
     3001 gaaaatcagg atacatttgt gaaaataatt cgaaatgata agaaaatacc tcttatggct
     3061 catttttatt ccgactttat gtacgaattc cggttacgga caatccgttt cggcgccact
     3121 ggcaagaccg aagctggttg tgggtatagt agtggaccag atgcgctggg actatcttta
     3181 ccggttttac aaccggtacc aggatggtgg atttaaacgg ttgattaacc agggattttc
     3241 ctgcgagaat acgctgatca attatttgcc ttcatatacc gccgtgggtc atacctgtgt
     3301 ttttaccggc tccgttccat ccatcagcgg gatttccgga aacgactgga ctgatcagct
     3361 cagtggcgcc cgggtatatt gcacagacga cagtacggta caggctgtcg gcgcgcctgg
     3421 ttctccggcc ggcaaaatgt cgccgcgtaa tctactggtc agcaccgtga cggatgaatt
     3481 acgcatcgct acaaattttc aatcccgggt ggtgggggtc tccttaaagg accgggcttc
     3541 catacttcca gccggacatg ccgccaatgc tgcctt
//
LOCUS       DASYCU010000512         3824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3878858, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000512 DASYCU010000000
VERSION     DASYCU010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3878858"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VGM24_10295"
     CDS             complement(<1..494)
                     /locus_tag="VGM24_10295"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY4155810.1"
                     /translation="MVKERLFGEFFNSEKAGGLLLIGCTLISLILANSPAGEGYIHFW
                     HMDASLQVPGFRIPFRIEEWINDGLMTVFFLLVGLEIERELYIGELSEFRNAILPAAA
                     ALGGMVVPATIHFLFNHGTAEQAGVGIPMATDIAFSLGVLSLLGNRVPLSFKIFLTAL
                     AIID"
     gene            583..1893
                     /gene="rimO"
                     /locus_tag="VGM24_10300"
     CDS             583..1893
                     /gene="rimO"
                     /locus_tag="VGM24_10300"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEY4155811.1"
                     /translation="MKTKSIRRDKVNIITLGCSKNMVDSEVLSGQLRANEIDVIHENA
                     RNDHNIVVVNTCGFIDKAKEESVNTIIEQVSLKKKGKLDRVYVTGCLSQRYREDLEKE
                     IPEVDAWFGTMELPFLLKKFNADYKAELLGERLLSTPRHYAYLKISEGCNRTCAFCAI
                     PLMRGRHASKTMEDIVQEAEGLVRMGVREIMLIAQELTYYGLDIYRKRALPELLNRLA
                     DIKGLEWIRLHYAYPSKFPLEIIDVIRDRENICNYLDMPLQHASDSMLKAMRRQITRS
                     EMTGLIHSIREKIPEICLRTTLIAGFPGEREQDIDELKDFLQEHRFDRVGIFTYSHEE
                     GTGAFDLKDDVPPAEKERRAQEIMDLQQGISYERNREKIGKTFKTLIDKKEAGRYIGR
                     TEFDSVEVDNEVIIASDIALPVGEFVQVKISKAYDFDLEGEVVE"
     gene            1983..3590
                     /gene="bshC"
                     /locus_tag="VGM24_10305"
     CDS             1983..3590
                     /gene="bshC"
                     /locus_tag="VGM24_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEY4155812.1"
                     /translation="MNFIANTLPYAQTGYFSKLVTDYLNGSDFLKSFCAHPVSYAGIE
                     AAIHNRELFPTDRKTLVEVLQDQYRDQPENEAVNRNIRHLLDKNSFTVTTAHQPAIFT
                     GSLYFIYKILHVVKLAEDLSARYPDKQFIPVYFMGSEDADLDELGHIYLDREKISWDT
                     PQTGAVGRMNTKGLDRVIQRIEGEFASHPFGPELIQLLKTAYLESESIQAATFKLLHQ
                     LFAEYGLVVLIADDRRFKAAMTTVFKDDLIHHVPFQITEKTVIRLSEHYTAQANPREI
                     NLFYLKDDIRERIDLKDGIYKVYNTGISFSPEALEKELGLHPERFSPNVILRGLFQET
                     ILPDIAFVGGGGETAYWFELKDLFQHYRVPFPVLVLRNSFLIIRDCLKAKMEKAGLTP
                     ETVFQPEDELLARIVKTNSSNQLNLKKEIGQLNAYYDSLKQISDAVDETLVQHVEALR
                     SKALKGITELEKKILRAEKRNFSDTRIRIQEIRETLFPLNGLQERIENFIPWYAEYGK
                     KFIDHIYAHSLRLEQEFVILEEQDHAV"
     gene            complement(3604..>3824)
                     /locus_tag="VGM24_10310"
     CDS             complement(3604..>3824)
                     /locus_tag="VGM24_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HEY4155813.1"
                     /translation="NTDLLIVGESSEWETPEYIRDARRFGKPVSMIILGHAFSEEPGM
                     EYLVQWLQPKVPGLKISHVASGEAFTWV"
BASE COUNT         1071 a          886 c          973 g          894 t
ORIGIN      
        1 tcatctatga tggcaagcgc agttagaaaa attttaaaag aaaggggtac ccggtttccc
       61 aagagggaaa ggaccccgag tgaaaaggca atatccgtag ccattggaat cccgactccc
      121 gcctgttccg cggtaccgtg attgaaaagg aaatggatgg tagccggtac aaccattcca
      181 cccagggcgg ccgctgcggg cagtatggca ttcctgaact cagacaattc cccgatgtat
      241 aattctcttt caatttccag gccaaccagc aaaaagaaaa cggtcatgag tccgtcattt
      301 atccattcct cgatccggaa cggaatgcgg aaaccgggga cctgcagaga agcatccata
      361 tgccagaaat gaatgtaccc ttccccggca ggactgtttg ccaggatcag cgaaatcagg
      421 gtgcagccga tcaggagtaa accgccggct ttttcactgt taaaaaactc tccgaataat
      481 ctttctttta ccatggtcaa aagtagaatc taagataagt attgttggcg accggaagca
      541 ccaaaggaga ttggctgtaa attctgctta cctttgccgc gcatgaaaac aaagtcgatc
      601 cggagggata aagtgaacat cattacgctg ggctgcagca agaatatggt tgacagtgaa
      661 gtgctcagcg gccagctccg ggccaatgag atcgatgtga ttcatgaaaa tgcacgaaac
      721 gaccacaata ttgtggtggt caatacctgc ggattcatcg acaaggcaaa ggaagaatcg
      781 gtcaacacca tcatagaaca ggtgtccctg aaaaaaaagg gaaagcttga cagagtgtat
      841 gttaccggat gcctgagcca acgctaccgg gaagatctgg aaaaggagat tcccgaagtg
      901 gatgcctggt ttgggaccat ggaactcccg tttctgttga agaaatttaa cgcggactat
      961 aaggcggaac tgcttgggga aagattattg tcaacgcccc gccactatgc ttacctcaaa
     1021 atttcagaag gctgtaaccg aacctgcgct ttttgcgcca ttccgctgat gcgtggccgg
     1081 catgccagta aaaccatgga agatattgta caggaagccg aggggctggt ccggatggga
     1141 gtaagagaaa taatgctgat cgcccaggaa cttacgtatt acggattgga tatttaccgg
     1201 aaaagagcct tgcccgagct gctgaaccgg ctggcggata ttaaaggact ggaatggatc
     1261 cggctccatt atgcctatcc ttcaaaattt cctttggaga tcattgatgt gatccgcgac
     1321 cgggagaata tttgcaacta cctcgatatg cctttgcagc atgcaagcga ttccatgcta
     1381 aaggccatgc gccggcagat tacccgcagt gaaatgaccg gcctgattca ttcgatccgg
     1441 gaaaagattc cggagatttg cctccgcacc accctgattg ccggtttccc cggggaaagg
     1501 gaacaggaca ttgatgaatt gaaggatttt ttgcaggaac accggtttga ccgggtgggg
     1561 atttttactt attcccacga agagggcacc ggggcctttg acctgaagga cgatgttccg
     1621 ccggctgaga aagagcgccg ggcacaggaa atcatggacc tgcagcaggg aatttcctat
     1681 gaaaggaacc gggaaaaaat cggaaagaca ttcaagaccc tcattgataa aaaggaagca
     1741 ggccggtata tcggaagaac cgaatttgat tccgtggaag tggacaatga agtgatcatc
     1801 gcatcggata ttgccctgcc ggtgggtgaa tttgtacagg tgaaaatatc caaagcgtat
     1861 gattttgacc tggagggaga agtggtggag tgatcaacgc atcccattga acaaaaaaaa
     1921 cgttctgttg ttagattagc gctgaaacag gaatgttcgt agatatctga ctttatcggc
     1981 ttatgaattt tattgccaac acactgcctt atgcgcagac cgggtatttt tccaaactgg
     2041 taaccgatta tctgaacggc agtgatttcc tgaaatcttt ttgtgctcac cctgtttcct
     2101 atgcgggcat tgaggcagct atccataacc gtgaattatt cccgaccgac cggaaaacgc
     2161 tcgtggaggt gctgcaggat cagtatagag atcagcctga aaacgaggcc gtgaatagaa
     2221 atatccggca cctgctggat aagaacagct tcacggtcac gacggcccac cagccggcca
     2281 tctttaccgg cagcctctat ttcatttata aaattctgca tgtcgttaaa ctggcggaag
     2341 atctctccgc aagatatccg gataaacaat tcattcccgt ttattttatg ggaagtgaag
     2401 acgcggacct ggatgagctg ggccatattt acctggacag ggaaaagatt tcctgggata
     2461 cgccgcagac cggggccgtg ggaagaatga atacgaaagg actggacagg gtgatacaac
     2521 ggatcgaggg agagtttgca tcccacccgt tcggaccgga attaattcaa ttgctgaaaa
     2581 ccgcttacct cgaatcggag agtatacagg ccgccacctt taaattgctg catcagcttt
     2641 ttgcagaata cggactggtc gtactgatcg ccgatgaccg gcggttcaag gccgccatga
     2701 caaccgtttt taaagacgac ctgatccatc acgtcccctt tcagattacg gaaaaaacag
     2761 tcatccgttt gtctgaacac tataccgcgc aggccaatcc ccgggagatt aatctttttt
     2821 accttaagga tgatatccgc gaacggatag atcttaagga cggaatatat aaggtgtata
     2881 ataccggaat ctctttttcc ccggaagcgc tggaaaaaga gttgggtttg catcccgaac
     2941 gattcagccc gaatgtgatc ctccggggtc ttttccagga gaccattctt ccggatatcg
     3001 cttttgttgg cgggggcggt gaaacggctt actggttcga actgaaggac ctgtttcaac
     3061 attaccgggt tccctttccg gtcctggtgc tgcgcaattc atttttaatc atccgggatt
     3121 gcctgaaagc aaaaatggaa aaagccggac ttaccccgga aacggtattt caaccggaag
     3181 atgaattgct ggcccggatc gtaaaaacaa attcttcgaa ccagcttaat ctgaaaaagg
     3241 agatcgggca attgaacgcc tattatgatt ccctgaaaca gatttcggat gccgttgacg
     3301 aaacgctagt tcagcacgtg gaagccctgc gaagcaaagc cctgaaagga ataaccgaac
     3361 tggaaaaaaa aatcctgcgg gctgagaaga gaaatttttc tgatacccgg atccggatac
     3421 aggaaatccg cgaaaccctt ttccctttaa acggccttca ggaaagaatt gaaaatttca
     3481 ttccctggta cgctgagtac ggaaaaaaat tcattgacca tatttatgcc cattccctta
     3541 ggctggaaca ggagtttgta atcctggaag aacaggatca tgcggtttga tcccggatgg
     3601 gcgctaaacc caggtgaagg cttcccccga agcaacatgc gaaattttta gtcccggcac
     3661 tttgggttgc agccattgca ccagatactc cattcccggt tcttcgctga aagcgtggcc
     3721 gagaatgatc atggaaacgg gtttgccaaa acggcgggca tcgcgaatat attccggcgt
     3781 ttcccattcg ctggattcac ccacgatcag gagatccgta tttg
//
LOCUS       DASYCU010000513         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4606735, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000513 DASYCU010000000
VERSION     DASYCU010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4606735"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..752)
                     /locus_tag="VGM24_10315"
     CDS             complement(45..752)
                     /locus_tag="VGM24_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4155814.1"
                     /translation="MTETVRKSILLVEDEENLHDALRLNLELENYQVTSAYDGNQALK
                     AVKEEYFDLVILDVMLPGMDGINVAETIRVQQNPVPILMLSARNTSADRVLGLKKGAD
                     DYLNKPFNLEELLLRIHKLIDKNQKLLEKNTAPEKYYFGKNGIDFKAQEAFNKKNEHF
                     RLSKKETMLLKLLIENKNEVVKREKILQAVWGYQVYPNTRTIDNFILNFRKYFEEDSR
                     NPKYFHSVRGVGYKYTE"
     gene            complement(749..1738)
                     /locus_tag="VGM24_10320"
     CDS             complement(749..1738)
                     /locus_tag="VGM24_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4155815.1"
                     /translation="MAITIYKKKRINRPNLVYWFLLLYIIAALGWWFIALNIQNRQMT
                     GYKLSLLKQDDPAYIQKAAHINNERDRKTAGYIGEGLFILIVILVGAVFLYSAIRRQN
                     RIHRQQQNFMMAITHELKTPISIARLNMETLMRHQLNDAQKEKILKSSLQEINRLNTL
                     TGNILVSAQLEGGSYRFDKELLDISRIVNDSSGEFISRFPARPWVREIEEGIRFNGDP
                     LLMQILVNNLIENAVKYSGPDSPVSILLNKQDNRGVLQVKDEGMGIPEKERKRVFQKF
                     YRVGNEDTRSTQGTGLGLYLVSKIARDHRMIIRLTGNHPQGSIFTVFFNLNPA"
     gene            complement(1776..2318)
                     /locus_tag="VGM24_10325"
     CDS             complement(1776..2318)
                     /locus_tag="VGM24_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HEY4155816.1"
                     /translation="MYFNTPAFADCGADSSGRLMPNAFKVKKITFLSMALFFLNACSF
                     TTGVFEKSIPLPGHEWASSFRPDIAFTITDTVSAYNIYIVLRHNDAYHYNNLYLRATV
                     KEPGESRPKSGDYELALATNEKGWIGTAMDDIYDARLLIQPKTKFSKAGTYHFIIEQL
                     MREDPLKSMLSIGLRVEKIQ"
     gene            complement(2308..>2795)
                     /locus_tag="VGM24_10330"
     CDS             complement(2308..>2795)
                     /locus_tag="VGM24_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEY4155817.1"
                     /translation="FSKVVGMAIDDLNDHRLNEGKVLEDDLILRIDNISVQQEEILKL
                     QPLRQQKIRDGLEKLLEEHVGKDHYDNNRLEQEMIYYLEKIDISEEQVRLKNHCAYFK
                     QVLAEPEDSKGKKLSFILQEIGREINTTGSKAYDVTIQRCVVLMKDELEKAKEQILNV
                     L"
BASE COUNT          610 a          674 c          677 g          834 t
ORIGIN      
        1 cgctggacga agcagagagc gtggtgggaa tgctgaacgc atagctattc cgtatactta
       61 tagcccactc cccgcaccga atggaagtat ttcggattcc ggctgtcctc ttcgaaatac
      121 tttctgaaat tcaaaataaa attgtcgatg gtccgggtat tgggatagac ctggtatccc
      181 caaacggcct gcaggatctt ttcccgcttt accacttcat tcttgttttc gatgagcagt
      241 ttgagcagca tggtctcttt tttgctcagc cggaaatgct catttttttt gttgaacgct
      301 tcctgggctt tgaaatctat cccgtttttt ccaaaataat atttttcggg cgccgtattt
      361 ttttccagca gcttttgatt tttgtcgatg agtttatgta tccggagcag cagttcttcc
      421 agattaaacg gcttgttgag gtagtcgtcg gctccctttt tcaatccgag tacccggtcg
      481 gcgctggtat tgcgggcgct cagcatcaag atcggaaccg gattttgctg aacccggatc
      541 gtttcagcca cgttgatgcc gtccatgccc ggaagcatca catccaggat caccaggtca
      601 aagtattctt cttttacggc cttcagcgcc tggttgccat cgtaagcgga agtgacctgg
      661 tagttttcga gttccaggtt gagccggagg gcgtcatgca ggttctcttc atcttcaacc
      721 agtaagatgg atttgcgcac tgtttccgtc atgccgggtt taaattaaaa aagactgtaa
      781 aaatacttcc ctgcgggtga ttgccggtaa gccggatgat catccggtga tcccgcgcaa
      841 ttttactgac taaataaagg cccaggccgg ttccctgggt ggaacgggta tcttcatttc
      901 cgacgcggta aaatttttga aatacccttt tgcgttcttt ttccggtatg cccattcctt
      961 cgtcctttac ctgtaaaacc ccccggttat cctgtttgtt cagaagaatg gaaaccggcg
     1021 aatccggtcc cgaatactta accgcattct cgatcaggtt gttgacgagg atctgcatca
     1081 gcagggggtc tccgttgaag cggatgccct cttcgatttc ccggacccag ggccgggcag
     1141 ggaaacgtga gataaattct cccgatgaat cgtttacgat ccgggaaatg tccagcaatt
     1201 ccttgtcaaa ccggtaagag ccgccctcca gctgtgccga aaccaggata ttccctgtta
     1261 gggtattgag ccggttaatt tcctgtaggg aggatttcag gatcttttct ttctgtgcat
     1321 catttaactg atgcctcatg agggtctcca tatttaggcg ggctatggaa atgggcgttt
     1381 tcagctcgtg ggtgatggcc atcataaagt tctgctgctg gcggtgaatc cggttttgcc
     1441 tccggatggc gctgtataaa aagacggcac ctaccagaat cacgatgagg atgaataagc
     1501 cctcaccgat ataacccgcc gttttccggt cccgttcatt atttatgtga gctgcctttt
     1561 gaatataggc cgggtcatcc tgtttgagca ggctgagctt atatcccgtc atttgccggt
     1621 tctggatatt cagggctatg aaccaccatc ccagcgcagc gatgatatac aggagcagga
     1681 accagtaaac caggtttggc cggttgatcc tttttttttt ataaatggtt attgccatga
     1741 tgctgcgcag gttttaattt tttgcagccc cggatttatt gaatcttttc cacccgcagc
     1801 ccgatactga gcatgctttt cagcgggtct tcccgcataa gctgttcgat aatgaaatga
     1861 taggtgccgg ctttgctgaa ttttgttttg ggctgaatga gcaggcgggc atcataaata
     1921 tcatccatgg ccgttccgat ccatcccttt tcgttggtgg ccagcgccag ttcgtagtcg
     1981 ccggatttcg gacggctttc ccccggttct tttaccgtag cccggagata cagattatta
     2041 taatggtagg catcgttgtg gcggagtacg atatatatat tatatgcgga aacggtatcg
     2101 gtaatggtaa aggcgatatc gggcctgaac gaagatgccc attcatggcc cggaagcggt
     2161 atgctttttt caaaaacgcc ggtggtaaag gaacaggcgt tcaggaaaaa aagagccata
     2221 cttaaaaagg ttattttttt cactttaaag gcgtttggca ttaaccgtcc ggaactgtcc
     2281 gcgccgcaat cggcgaaggc aggcgtatta aagtacattc aatatttgct cctttgcttt
     2341 ttccagttcg tccttcatca agaccacgca gcgctggatg gtcacatcgt atgctttgga
     2401 accggtggta ttgatttccc ttccgatttc ctgaaggata aaagagagtt ttttcccctt
     2461 actgtcttcc ggttcggcca gcacctgttt aaaataggcg caatggtttt tgaggcggac
     2521 ctgctcttca ctgatatcta ttttctccag gtaatatatc atttcctgct ccagccggtt
     2581 attatcgtaa tggtccttgc ccacatgttc ttccagcagt ttttccagac cgtcccggat
     2641 cttctgctgg cgcaggggct gaagctttaa gatttcttcc tgttgcacgg atatattatc
     2701 gatgcgaagg atcaggtcat cttccagtac tttgccctca ttcagccggt ggtcattgag
     2761 gtcatcaatg gccatcccca ccaccttgct aaact
//
LOCUS       DASYCU010000514         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2284804, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000514 DASYCU010000000
VERSION     DASYCU010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284804"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGM24_10335"
     CDS             complement(<1..368)
                     /locus_tag="VGM24_10335"
                     /inference="COORDINATES: protein motif:HMM:NF015604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HEY4155818.1"
                     /translation="MKRIFFILLSLPFFASSQSFTLVSQGKSNSRIVIPEKAGIVEIQ
                     AAKVLQDYIERMSGATLPIVGDEARPASGEVLIGRVKREGMENIPFDKLGKDGLFIKS
                     AANRLIIAGGTGNGVLYGVYT"
     gene            480..923
                     /locus_tag="VGM24_10340"
     CDS             480..923
                     /locus_tag="VGM24_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155819.1"
                     /translation="MTRHFLFSAAILFISISAFCQDAGSALQGCEPYFDTLTSRQLYR
                     APEKDAEYKGGTEKFANYINQNFIYDKSHVDRETSLIFTFIVEPDGSVSNVSLLRPAR
                     GVADDVIERGMLVMEKMPKLKPAICHNQKVPQRLSQPITVSPAGN"
     gene            946..1527
                     /locus_tag="VGM24_10345"
     CDS             946..1527
                     /locus_tag="VGM24_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY4155820.1"
                     /translation="MHKPFIDHVLRFISLTAAERQLITAKIPSLAVRKKDFLLRAGQI
                     CTRRFFVEKGCLRQFFINKKGEETIIQFAIENWWISDYMSLTDHTAAHFSIQAVEDSS
                     VLVLDDDHYESLLRACPKMEKYFRIVLQKAYAAAQFKFRLMHDFSKEERYRFFNKHFP
                     EFVQRVPQYMLASFLNITPEYMSKIRSQHLSIS"
BASE COUNT          410 a          399 c          366 g          397 t
ORIGIN      
        1 gtgtacacgc cgtacagcac cccgtttccg gtaccaccgg cgatgatgag ccggtttgcg
       61 gcggacttga tgaacaagcc atccttgccc agcttatcaa aaggaatgtt ttccatgcct
      121 tcgcgtttca cccggccaat aagcacttca ccggacgcgg gtctggcctc atcgccgacg
      181 atcggcagtg tggcgccaga catccgctca atataatcct gcaatacctt ggcagcctga
      241 atttccacga taccggcctt ttccggaatc acgatccggc tattcgattt tccctggctg
      301 accagtgtaa aggattgtga actggcaaaa aaaggcagag aaaggagaat aaagaaaatg
      361 cgcttcatga ttattcagga tttggtgcag cggaatcaat aaatcgacta aatttaagtt
      421 attgccctgt ttcgaagaag ttttttctga cgatgatgac ggaaatatca tttatcacca
      481 tgacgcgaca ctttctattt tcagcagcca ttctttttat cagcatttcc gctttctgcc
      541 aggacgcagg ttccgcattg cagggttgtg agccttattt tgacacactg accagccggc
      601 agctttatcg ggctccggaa aaagacgcgg aatacaaggg cgggacagaa aaatttgcca
      661 actatatcaa ccagaatttt atttatgaca agagccatgt ggatcgcgag accagtctca
      721 ttttcacctt tatcgtggaa ccggatggtt cggtaagcaa cgtcagcctg cttcgtccgg
      781 cccggggtgt tgcggatgat gtaatcgaac ggggcatgct cgtgatggaa aagatgccga
      841 aattaaaacc agcgatttgt cacaaccaga aagtacccca gcggcttagc cagccgatca
      901 cggtttctcc agcgggcaac tgatcctttg caaaaaacat tgctgatgca caagcccttc
      961 atcgatcatg ttttacgctt catttcgctg actgcggcgg agcggcagct gatcaccgct
     1021 aaaattccct cgctggccgt gaggaaaaag gacttcctgc tccgggccgg ccagatctgc
     1081 accaggaggt tctttgtgga aaaaggctgc ctccggcaat tcttcatcaa caaaaaaggc
     1141 gaagagacca tcattcaatt tgccatagaa aattggtgga tctccgatta tatgagtctg
     1201 acggatcata ccgcagccca tttttcaata caggccgtgg aagactcttc cgtcctggta
     1261 ctggatgacg atcactatga atccctcctg cgggcctgcc cgaaaatgga gaagtatttt
     1321 cgcatcgtgc tacaaaaagc gtatgcggcg gcccaattca agttcagatt gatgcatgac
     1381 ttttctaagg aagaacgata ccggtttttc aacaaacatt tccccgaatt tgtacaacgg
     1441 gttccacagt atatgcttgc ttccttcctg aatatcaccc ccgaatacat gagcaagatc
     1501 agaagccagc atctcagcat ttcttaacct ggattaagta tttcccggcc ttcaggtggt
     1561 aactttgtga ca
//
LOCUS       DASYCU010000515         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2576287, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000515 DASYCU010000000
VERSION     DASYCU010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576287"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VGM24_10350"
     CDS             complement(<1..280)
                     /locus_tag="VGM24_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155821.1"
                     /translation="MNSKNLKTTGGQVHLNSADYWVETHGFGMVFRLVNGIVNQVRCP
                     AECETPLLTKGTKMKIVIFIYNGITMLDAIGPYEVLKDIDDAEVFFVAE"
     gene            368..1219
                     /locus_tag="VGM24_10355"
     CDS             368..1219
                     /locus_tag="VGM24_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEY4155822.1"
                     /translation="MLLNLPRQSDLRVRIFLLFSFFVTGFTLPSQAQPGRGKRKSAFT
                     ARLMNIEAAVKDPFRYNTTLYNGSGYTQIYELQTGAPEGWITMFRVSGSLVAALKLDT
                     GKTQEITVELTAPPETKPGKYNIPVTAVSNGDSLRLDLEAVVKGNYSIELSTPTGRLS
                     DEITAGKNKPIHLIVKNKGSLPLIGLELSAESPLQWSATFEPSKIERLDPGQTKEVTA
                     TLSVPDKTIAGDYVTNFTARNSSTTATAVFRMTVVTSLLSGWIGILIILISLGIVYYL
                     VRKYGRR"
     gene            1226..2266
                     /locus_tag="VGM24_10360"
     CDS             1226..2266
                     /locus_tag="VGM24_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4155823.1"
                     /translation="MENPIIELAGLTKNYGSVKAVDNLYLKIDKGEIFGLLGPNGAGK
                     TTTILMMLGLAEPAAGIARVCGVNATRNPIAVKRKVGYMPDSVGFYDSMTAIENLMYI
                     GRLNGIAEYEIEARAQKTMEIVGLGEEMDKKTSAYSRGMKQRLGLADILIREPEVIIL
                     DEPTLGIDPSGIKEFLSLIRRLSRQQGLTVLLSSHQLHQVQQVCDRVGIFVDGRLLVE
                     GNMETLSRNLFGTESYVVLITVREPLPADWEPADELRKWNEISHITIRENTVELSSRQ
                     DITPDIVRFFVQKGLNITGVHKKEFGLDEIYHRYFENNQKKHIAHGKSTGLFQRSLFK
                     RNNAKQADAAED"
     gene            2283..>2675
                     /locus_tag="VGM24_10365"
     CDS             2283..>2675
                     /locus_tag="VGM24_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY4155824.1"
                     /translation="MVRKETGDHIRSWRFIVMLLLIGFTFLSSLYVSLSNLNKAVSNL
                     KDPDRIFIYLKLLTVSDGTLPTFHIFIGFLGPLLGIALGFDAVNAEQNNGTLTRLMAQ
                     PVYRDNLLLAKFWAGLTVVSVLFLCLALL"
BASE COUNT          692 a          659 c          674 g          650 t
ORIGIN      
        1 tttcagcaac aaaaaacact tcggcgtcat cgatgtcttt taatacttca tagggcccga
       61 tggcatccag catcgtaatt ccgttatata taaaaattac tattttcatc ttagttcctt
      121 tagtgagtaa tggcgtttcg cactccgccg ggcagcggac ctgatttaca ataccattaa
      181 ccagccgaaa aaccatacca aatccatgcg tctctaccca ataatctgcg ctgtttaaat
      241 ggacctgacc gccggttgtt tttaaatttt tagaattcat taacaaaacc atttttcact
      301 gcttgttatt ttcgttcaac cattcagttc ttattttatc agtgaacaat caatatttct
      361 taacgccatg ctactgaacc ttccccggca gagcgatctg cgtgttcgta tttttttgtt
      421 attttctttt tttgttaccg gttttacgtt gccctcgcag gcccagccag gcaggggaaa
      481 acggaaatcg gcttttacgg cccgcctgat gaatatcgaa gcggcagtga aagacccctt
      541 ccggtataat acgaccttat acaacggcag cgggtatacg cagatatacg aattgcagac
      601 cggggcgccg gaaggctgga tcacgatgtt ccgcgtcagc ggcagcctgg tggccgcgct
      661 caaattggat accggtaaaa cccaggagat cacggttgaa ctgacggccc ctccggaaac
      721 gaagccggga aaatataata taccggttac tgcggtcagc aacggagatt ctctccggct
      781 tgacctggaa gccgtggtga agggcaatta cagtattgaa ctcagcaccc caacaggacg
      841 actgagtgac gagatcaccg cagggaagaa taagcccatt cacctcatag tgaagaacaa
      901 gggctccctt ccgctgattg gattggaact ctccgcagag tcgcctttgc aatggagcgc
      961 aacgtttgaa ccttcgaaaa tcgaacgcct ggatcccgga caaacaaagg aagtgacagc
     1021 aacgctgagc gttcccgata aaacgattgc gggggactat gtcaccaact ttactgccag
     1081 gaacagcagt acgacggcca ccgccgtatt ccgcatgacc gtggtcactt cgctgctttc
     1141 cggatggatc ggcatactga taatacttat ttccctggga atcgtgtatt acctggttcg
     1201 taaatacgga agaagataac gcatcatgga aaatccgatt attgaactgg cgggcttgac
     1261 aaaaaattac ggttcggtga aagccgtgga caatctctat ctgaaaattg acaaagggga
     1321 gatcttcggt ttactgggcc ctaacggagc cggcaaaacc actacgatac tcatgatgct
     1381 ggggctggcg gaacccgcgg ccggcattgc ccgcgtttgc ggcgtgaatg caacacgcaa
     1441 tccgattgcg gtgaagcgga aagtgggata tatgcccgac agcgtgggtt tttatgatag
     1501 catgacggcg attgaaaacc tgatgtatat cggcaggctc aatggaatcg cggaatatga
     1561 aatagaggca agggcacaga aaacgatgga aatcgtcggt ctgggcgaag agatggataa
     1621 aaaaacttca gcctactccc gcggtatgaa acagcgttta ggccttgccg atatactgat
     1681 ccgggagccg gaagtgatca ttctcgatga accgaccctc ggtatcgacc ccagcgggat
     1741 aaaagaattt ctctccctca tccgccggct cagccgccag caggggctca cggtcttgtt
     1801 gtcgtctcat cagctgcacc aggtgcagca ggtttgcgac cgcgtgggca tttttgtgga
     1861 cggcagactg ctggtggaag gcaatatgga aacgctctcc cggaatttat tcggaacaga
     1921 atcctatgtg gtgctgatca ccgtccggga gccgctgccc gcagactggg agccggccga
     1981 tgagctccgg aaatggaatg agatcagcca tattaccatt cgggaaaaca ccgtcgaact
     2041 gtcgagccgg caggatatca cgccggatat cgttcgcttc ttcgtgcaaa aaggattgaa
     2101 tataaccggt gtgcacaaaa aggaattcgg gttggacgaa atttatcacc ggtattttga
     2161 gaacaatcaa aagaagcata ttgcccatgg aaagtcaact ggcttatttc aaagatctct
     2221 tttcaaacgg aataacgcaa aacaggcgga tgcagcggaa gactgaaacc ccgttttggg
     2281 taatggtacg caaagagacg ggcgaccaca tccgcagctg gcgctttatt gtcatgctcc
     2341 tgctgatcgg gttcactttc ctgagttcat tgtatgtatc cctgagcaat ctgaataaag
     2401 ccgtgagcaa cctgaaagat ccggaccgga tctttattta cctgaaatta cttaccgtat
     2461 cggatggaac gcttccgacc tttcatattt ttatcggctt cctggggccc ttactcggca
     2521 ttgcgcttgg tttcgatgcc gtgaatgcag aacagaacaa cggtaccctg acccgcctga
     2581 tggcgcagcc ggtttaccgc gataaccttt tgctggcaaa gttttgggcc ggtctcacag
     2641 tggtcagcgt gttatttctt tgcctggccc tgctg
//
LOCUS       DASYCU010000516         4590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_399979, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000516 DASYCU010000000
VERSION     DASYCU010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399979"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1705
                     /locus_tag="VGM24_10370"
     CDS             <1..1705
                     /locus_tag="VGM24_10370"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155825.1"
                     /translation="AITVFLLMTMSCKKHLLDITPTTGVSGTTLFSDPNLTEALVTNN
                     YRDLTYGFYNCHWQAFMLTGATDETLSAYESYSGVNIINKGLLDPTPNGEGFTGTFDG
                     NPYPRQQIWANNYSYISQENSFFENIANVPGLSQDDKNRLTGEMKALRAFRYFTLVKH
                     YGGVPIITRTYGLNDSFSVSRNTVDECFQFILGQLDSAITLLTGIASNAGKIDQGVAM
                     AIKSRVLLFYASPLFNTTNDASRWQKAADAAQAVIDLGRYSLVPWNEYKSMFIKYSPA
                     NKENIFITINEAGIASEGHQDDYPWPGLTFEYCVMPFSRGGNIMYTPTQQMVDSYETL
                     DGKAITDPSGNYDPQNPYANRDPRLDIDILHQGSALNQSTIDFRSPDGLDFNQNQTVT
                     GYLLGKFVDPSVYDATGVTIGSGQGNQPWIYMRYAEILLNYAEAINEAQGPASAYAPI
                     NLIRNRAGMPDLPTGLSQTQMRLKIQNERKVELAFEEHRFWDVRRWKIAGTVNNFTAM
                     GVSVTESGGVLHYAYAVPNTGGNLDYDPVRVFKDPANYFFPIPLSEIAADPQLTQNPG
                     Y"
     gene            1787..3481
                     /locus_tag="VGM24_10375"
     CDS             1787..3481
                     /locus_tag="VGM24_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155826.1"
                     /translation="MKRREAIAKVNWMLGGSFIGLNWYHKAVIDPWIEETGPTGTRPE
                     TYFLQEAVNLGLAAIAHRLDKADHFRPYFEIHLKPDPFLEHQIWDFGDMCSRFTDAYI
                     LGRQMTGFDEFRVEELALRKLLLKSDPYANPFMAGRMLIAYVDIYLKEPVPEHKKLIA
                     GLVETIRSKMTFEKDYAYYFKNADGWRSVHDAVFGDFNGYPTYPLGGIILALARYTEV
                     VKEPACEDLLNRLCRFVLEVSGTFEADGRFMGHTHSGGILTAAAGIMRWAIDRNDKKV
                     TGQMKNVFDWTMKHSSSWGWVPDGLGPDHASCESCSITDALHLGLLIARHLDSSYYGI
                     LERFSRNQLLENQIRSPEFLLPATPFPDRNRIENALYGSWASYSLPNSLDNSTDVEGC
                     CLGSGILGCFLVWDNIVIKKDDRVMVNMALSRNSPWLEVIGYQPYEGKIELLIHDAPK
                     LCVRIPEWVNEGEIRISSGGKILPFTFMPNRYIELNALKKGDLVRIEYPLKQEQRVET
                     VSGQSYDVRWRGDTVVSVKPLGQVYPIFRREWAERETAPMNAGDHYQKQLGVGVHW"
     gene            3702..>4590
                     /locus_tag="VGM24_10380"
     CDS             3702..>4590
                     /locus_tag="VGM24_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HEY4155827.1"
                     /translation="MAKNLEYRIYENGVPVLEPSAADLLLGDNQSFSINNSIRSHSIK
                     KIANEIISPVPEKRKKIKDDYSLISIVFRKPYKVEFRVYEDGVAYRFITSFKDSITVE
                     NEVASFRFFTSSAAYFPSIHKRDDADIFHTSFEELYPLRQLDSIKKTEVGYSPVLVTQ
                     PGKPKIAIAESDLEDYPGMFISGTGSDILEGIFAPYPLEEKMTGGDYPQSVVTKRANY
                     IAKTKGARTFPWRVLLIADEDRQLPSNDIVYRLASPSRIGGSSWVNPGKGTDEWTINV
                     NLFNVPFRSGVNTASYKYYI"
BASE COUNT         1289 a          929 c         1095 g         1277 t
ORIGIN      
        1 tgctatcacc gtctttttgt taatgacgat gtcatgcaaa aagcacctgc tggatatcac
       61 accgacaacc ggcgtttcgg gtactactct tttctccgat cccaatctta cggaagcatt
      121 ggtaaccaat aattacagag accttacgta cgggttttat aattgccact ggcaggcttt
      181 tatgcttacg ggagctaccg atgaaacatt atccgcttat gaatcctaca gtggagtcaa
      241 tattatcaac aaaggccttc tggatcccac gccaaatgga gagggtttta caggtacttt
      301 cgatggaaat ccatatccgc ggcagcaaat atgggcaaac aattatagtt acatttctca
      361 ggaaaattct ttttttgaga atattgcgaa cgttccggga ttatcccagg atgataaaaa
      421 cagattaacc ggggagatga aagcgcttag ggcattcagg tattttacgc tcgtaaaaca
      481 ttatggaggg gtacccatca ttacccgtac ctatggctta aatgatagtt tttccgtttc
      541 caggaacacg gtcgacgaat gttttcaatt cattcttggt caattggatt cggcaatcac
      601 cctgttgacc gggatagcct cgaatgcagg gaagattgac cagggagtag ctatggccat
      661 taaatcaaga gttcttttat tttatgccag ccctttattt aatacaacca acgatgcttc
      721 caggtggcaa aaagcggccg atgcagcgca ggctgtaatc gatttgggaa ggtacagcct
      781 ggtgccctgg aacgagtata agagcatgtt tattaaatac agccccgcaa acaaggagaa
      841 tatttttata actattaacg aggccggtat tgcatcagag gggcaccagg acgattaccc
      901 gtggcccggc ctcacttttg aatactgcgt aatgcctttt tcaagaggcg gcaatatcat
      961 gtatacgcct acccagcaaa tggttgattc ctatgaaacg ctggatggaa aggcaataac
     1021 agatccgtcc ggtaactatg atccgcaaaa tccttatgct aaccgtgatc cgagactgga
     1081 tattgatatt ttgcaccagg ggtcggcatt aaaccaaagt accattgatt tcaggagtcc
     1141 ggatggattg gacttcaacc agaaccaaac cgtaacgggt tatcttttgg ggaaatttgt
     1201 tgacccttcc gtatacgatg ccaccggggt aaccattgga tcagggcagg gtaatcaacc
     1261 gtggatttat atgcgttatg ccgagatttt attaaactat gcggaagcaa ttaatgaagc
     1321 gcaaggacct gcatcggcat acgcgccaat aaacttaata cgtaaccggg cgggtatgcc
     1381 cgatcttcca acaggattat ctcaaaccca aatgagattg aaaatccaga atgaaagaaa
     1441 agtggaactc gcttttgagg aacatcgttt ttgggatgtg cgaagatgga agatagcggg
     1501 gactgttaat aattttaccg ccatgggcgt atcagtaaca gaaagcggcg gcgtattgca
     1561 ctatgcttat gcagttccca atacaggtgg taacctggac tatgatcccg tcagggtttt
     1621 taaagatccg gccaattatt tttttccgat accactaagt gaaatagctg cggaccccca
     1681 actaacacaa aacccgggtt attaggcatt tgatcttttt cctgtttttt gatatatttt
     1741 cattaatact taccttataa aacctgcttt atcggagaat tcgtttatga aaagaagaga
     1801 ggcgatagca aaagtgaact ggatgctggg tggctctttt ataggcctga actggtatca
     1861 taaagcagtc attgatccct ggatagaaga aaccggacca acaggtacgc gacctgaaac
     1921 ttattttttg caggaagcgg tgaaccttgg tcttgccgct attgctcacc ggctcgacaa
     1981 ggctgatcat ttccgtcctt attttgaaat tcatttaaaa cccgatcctt ttttagaaca
     2041 tcagatttgg gattttgggg acatgtgctc tcgttttact gacgcctata tacttggaag
     2101 acaaatgacc ggctttgatg aatttagggt tgaagaactg gctctgcgca aattattatt
     2161 aaagagcgat ccctatgcca atccctttat ggcgggaaga atgcttatcg cctatgttga
     2221 catttatctt aaggagccag taccggaaca caaaaagcta attgccggtc tggttgaaac
     2281 aatacggtcg aaaatgacat ttgagaagga ttatgcgtac tactttaaaa atgcggatgg
     2341 ctggcgatcc gtgcatgatg cggtttttgg cgattttaat ggatatccca cgtatccgct
     2401 gggaggaata attcttgcgc ttgcccggta tacggaagtg gttaaggagc cggcatgcga
     2461 agatttattg aaccggcttt gcaggtttgt cctggaggtg agcggcactt ttgaagcgga
     2521 tggccgtttc atggggcata cgcattccgg aggtatatta acggctgctg cagggataat
     2581 gcgatgggca attgatcgca atgataagaa agtaactggt cagatgaaaa atgtttttga
     2641 ctggacaatg aagcattcga gtagctgggg ttgggtgccg gacggccttg ggccagacca
     2701 tgcatcctgt gaatcctgtt cgattacgga tgcgcttcat cttggattgc ttattgcgcg
     2761 ccacctggat tcttcgtatt acggaatcct tgagcgtttt tcccgaaacc agcttctgga
     2821 aaaccagatt cgcagcccgg aatttctcct tccggcaacg ccttttcctg accggaatag
     2881 aattgaaaat gcgctttacg gaagctgggc ttcctattca ttgccgaact cgctggacaa
     2941 tagcacggat gtggaaggat gttgccttgg atccggaatc cttggttgtt ttctggtgtg
     3001 ggacaatatc gttattaaaa aagatgaccg ggtaatggtt aacatggcgc ttagccggaa
     3061 ctctccatgg ctggaggtta ttgggtacca gccttatgaa ggtaaaatag aattactcat
     3121 tcacgatgct ccaaaactgt gtgttagaat cccggaatgg gtgaatgaag gagagattcg
     3181 gattagctcc gggggaaaga tacttccatt tacatttatg ccaaaccggt atatagagct
     3241 gaatgctttg aaaaaaggcg atcttgtgag aattgaatat cctttgaagc aggagcaaag
     3301 ggtcgaaacg gtttccggcc aatcctatga tgttcgttgg cggggggata cggtggtatc
     3361 cgttaagccg ttaggacaag tctatccgat cttccggaga gaatgggcgg agcgggaaac
     3421 cgctccgatg aatgcgggtg atcactatca aaaacaattg ggggtaggcg ttcactggta
     3481 aagagaggcc gggaaacaac cgttatttta tccacctgcc ggttgaaatg atattgattt
     3541 caggcggccg gtttaatttg taaattataa tgaattttaa attaaattct gtcagaacat
     3601 ttgttttagc catattattg agcatgggtt atttgtgtgg ccgctgtgaa gataccctgc
     3661 gtgtctgttc gccttcaggg aaaatatgcg taaaaatatg gatggcaaaa aacctggaat
     3721 accggatata cgaaaatggc gtgcccgtgt tagaaccatc agcggcggat cttttattag
     3781 gtgataatca gagtttctcc attaataaca gtatcagatc acactcaatc aaaaaaatag
     3841 ccaatgagat tatttctcct gtccccgaaa agagaaagaa aataaaagat gactatagtt
     3901 taatttcaat agtattcagg aagccatata aagtagaatt ccgcgtatac gaggatgggg
     3961 tggcttatcg gtttataacg tcttttaaag attcgattac cgttgaaaac gaagtcgcat
     4021 cgtttcgttt tttcacatct tctgctgcct attttccctc catacataaa agggatgatg
     4081 cagatatctt tcataccagc tttgaagaat tatacccgtt aaggcaattg gattcaatta
     4141 aaaagacgga agttggctat tcaccggttc ttgtaacaca gccgggaaaa ccaaaaattg
     4201 ccattgcgga atcagacctc gaagattacc ccggtatgtt tataagcgga accggatctg
     4261 atattttgga aggcatattt gctccttacc cgttagagga aaaaatgacc ggcggcgatt
     4321 acccgcagtc ggtagtgacc aaacgtgcga actatattgc aaagacaaaa ggcgcaagaa
     4381 cttttccgtg gcgtgtttta ctgattgccg atgaagacag gcaattacct tccaatgata
     4441 ttgtttaccg gttggcctct ccttcccgaa ttggtggttc atcctgggta aaccctggta
     4501 aaggcacgga tgaatggacc atcaacgtca atctttttaa tgttcctttc cgatccggcg
     4561 ttaatacagc atcctataaa tattacattg
//
LOCUS       DASYCU010000517         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1269652, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000517 DASYCU010000000
VERSION     DASYCU010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269652"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VGM24_10385"
     CDS             <1..290
                     /locus_tag="VGM24_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155828.1"
                     /translation="DYTYNASKKGIKVMISYNAKVYENVKLTSPGAVYNNPTLKSLLR
                     SFRNRYSINSLDKIFYYSIKHRGTVSGSLIAVLRVAGIIKNFVLVKIKNKA"
     gene            287..1309
                     /locus_tag="VGM24_10390"
     CDS             287..1309
                     /locus_tag="VGM24_10390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4155829.1"
                     /translation="MKILFLSHHWTNNSHHSKHSGFQRLVVHAAKENEVTLITWGREK
                     AEYTDEYGIHIITIKGSKRDFLFLKRMGISLKGRKVSENFDAVHALYSDCTFFLKKNS
                     FAVTFHVLPGIAAYPEFREKLFLFLKYNLLQKRAMRRAREIICVSKNLLSRIPEKYRG
                     KARFIPHGVDTEFWDPALSRSGEPRAENNVLCVGSHGLDKDLLKKIILGNPQTPFVIV
                     GLKNKLAEYPNVSYLYNLPDEKLRDLYSSSGAVLKPLAFSTANNSILEAMAMGTTILA
                     SRIPGVTDYLDDSNCLFIDTLNAFSLEGIESRKPDPGLVRESALRNFSWNSVLMAYLN
                     LYYQQK"
     gene            1311..2402
                     /locus_tag="VGM24_10395"
     CDS             1311..2402
                     /locus_tag="VGM24_10395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4155830.1"
                     /translation="MAKKRIAIIGLKGLPAFGGAANVGENIIEQLAGEYEFTVYATET
                     HTSVRGNYKGAQQIVFRKFPIGALNIFYYYIASAFHAVFKRNYDLVHLHQMDGAFILL
                     LLRLKYPVLATSHGLTYRTGKWSRLLYPYFKINEWFQARLSNHLTVVSRSLVTHYARL
                     ISPERISYIPNGITVSPVTGKGPDTKEYILFAAGRLIPIKGLHFLLSAMNECRYTGKL
                     IVLGDLAQMEEYSKELFRLSSGLDIEYRGLIRDRELLNAHIANARFFVFPSTQEAMSM
                     MLLEVASLKIPVICSDIIQNTDIFSPEEMLFFKSGSSPDLAAKLTWAMQNPDKMKIFS
                     SRAFETLLEKYQWSAISRQYDSLFKKIMA"
BASE COUNT          687 a          560 c          583 g          657 t
ORIGIN      
        1 cggactatac ctataacgct tcaaagaaag gcatcaaggt gatgatttcc tataatgcga
       61 aagtgtacga aaatgtaaaa ttaacttccc cgggcgccgt ttacaataac ccgacgctaa
      121 aatccctgtt gcggagtttt agaaacaggt attccattaa ttcattggat aagatatttt
      181 actattccat caagcatcgc ggcacggtca gcggatcttt aatagccgta ctgcgggtgg
      241 cgggaataat caagaatttt gtgctggtta aaatcaaaaa taaggcatga agatcttgtt
      301 tttatcccat cactggacca acaacagcca tcattccaaa cacagcggat tccagcggct
      361 ggtagttcat gcggccaaag agaatgaagt tacgctcatt acctggggaa gggaaaaagc
      421 cgaatatacc gatgagtatg ggatccacat tataaccatt aaagggtcaa agagggattt
      481 tcttttttta aaaaggatgg gtatctctct gaaaggcaga aaggtttcgg agaacttcga
      541 tgccgtccac gctttatatt cggactgcac tttctttctg aaaaaaaaca gctttgccgt
      601 tacctttcat gtactgcccg gcattgctgc ctacccggaa ttcagagaaa agctgtttct
      661 atttttaaaa tataaccttt tgcaaaaacg ggccatgcga agggccaggg aaattatttg
      721 cgtctccaag aatttattgt cccggattcc tgaaaagtat agggggaaag cccgtttcat
      781 tccgcatggg gtggacacgg aattctggga tcctgcctta tcccggagcg gtgaaccccg
      841 cgcagaaaat aacgtcttgt gtgtcggctc gcatgggctg gataaggatc tattaaaaaa
      901 aatcatactg ggcaacccgc agacgccgtt tgtgatcgtg ggcctgaaaa acaaactggc
      961 ggaatatcct aatgtttcct atctctataa cctgccggat gagaaattaa gggaccttta
     1021 ttcttcttcc ggcgccgtgc tcaaaccgct ggctttttca acggccaaca attccattct
     1081 ggaagcgatg gcgatgggaa cgacgatcct ggcaagccgg attccgggag tcaccgacta
     1141 cctcgatgat tcaaactgcc tgttcatcga caccctgaac gctttttccc tggagggcat
     1201 agaatcgcgc aaaccggatc cgggtttggt cagggaatct gccctgagga atttttcctg
     1261 gaacagcgtg ctgatggcat atcttaacct gtattatcaa caaaaatagg atggcaaaaa
     1321 aaagaatagc cattatcggt ctgaaaggcc ttccggcatt cggcggggcg gcgaatgtag
     1381 gggaaaacat aatagaacag ctggccggag aatatgagtt caccgtatat gccaccgaaa
     1441 cacatacttc cgtaagaggg aattacaaag gcgcccaaca gatcgtattc cgcaaattcc
     1501 ctatcggagc gctcaatatt ttttactatt atatcgcctc cgcctttcac gccgttttca
     1561 aacgaaatta tgatctggtc catcttcacc agatggatgg ggcatttatt cttctgctgc
     1621 tccgcctgaa atacccggtc ctcgcaacaa gtcacgggtt aacgtacagg acgggaaaat
     1681 ggagcaggct gctgtatccg tattttaaaa taaatgaatg gttccaggcc aggctgtcta
     1741 accatctcac cgtggtttcg cgatcactgg tcactcatta cgcgcggctg atttcgccgg
     1801 agaggataag ctatatacca aacggcatca cggtcagtcc ggtaactggg aaaggcccgg
     1861 atacaaaaga gtatattctt tttgcggcgg gccggctcat ccctataaaa ggtttgcatt
     1921 ttttgctttc tgcaatgaat gaatgccgtt ataccggcaa gctcatcgtg ctcggagatc
     1981 ttgcccagat ggaagaatat tcgaaggaac ttttccgtct ttcttccggg ctggatattg
     2041 aataccgggg tctgatcagg gacagggaat tgctgaacgc ccatattgcc aatgcccgct
     2101 tctttgtttt tccttccacg caggaggcga tgtcgatgat gctgctggaa gtagcttcgc
     2161 ttaaaatacc ggtgatttgt tccgatatta ttcaaaatac agatatcttc agtccggagg
     2221 agatgctgtt tttcaaatcg ggttcttccc cggatctggc tgccaaatta acctgggcga
     2281 tgcaaaatcc cgataagatg aagatcttct cttccagggc ctttgaaacc ctgttggaaa
     2341 aatatcaatg gtcggctatt tccaggcaat atgattcctt attcaaaaaa ataatggctt
     2401 gattttgcag aattcagtaa acaacatgta aagcggtatt catgaaacag gttgtcgaaa
     2461 caggagcggg tatgacggtc tctaaat
//
LOCUS       DASYCU010000518         4996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3591400, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000518 DASYCU010000000
VERSION     DASYCU010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3591400"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3299
                     /locus_tag="VGM24_10400"
     CDS             <1..3299
                     /locus_tag="VGM24_10400"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY4155831.1"
                     /translation="GKMVLEKTDSTRSNGLFTLDPESISRAPAGWYLVEFRAKDPNGE
                     TVTDKRYIELFRKEERKADYPTYNLVASDDQTGEPGKELTIKTGTSATGIYVIRAKEG
                     LQDSLTDYSFYQLNNEIKSTTLKVEEKDRGGFAVNDVFIKNNRWYSSTHTIHIPWTNK
                     ELQVSYETWRNKLLPGSTEEWKVKISGYKRNKVAAEVLTAMYDASLDQFKKQAWSIPD
                     LYPEFSGETSWSGISDFDDQGSMGRPVTEYQKENFYKQYDQRVSSEGRGIMVREEFSP
                     AAPVQAKYSGDFDLSPKKMTIGKEAEPAKQLPEAPNPGEQIRKNFNETAFFFPDLKTD
                     PEGDVSISFTIPEALTQWKWMIFAHTRDLAFGYSEKTVVTQKDLMLQPNLPRFFREGD
                     TMSLPVKISNLSTLAMNGTVSLDWLDAADNSNQNQALQKIARQPFRVEAGQSTVVYFP
                     AVIPKDFGQPVVYRLQAKTNSGKAGDGESGVIPVLSNRILVTETFPLNMAGQDNKEFS
                     WERLLNSRTSPTLQNQSLTVEYTSNPAWYAVQSLPYLMEFPYECAEQSFNRFYANALA
                     ARIINHAPVIRAIFEKWKNSDSSALLSNLQKNEELKTVLLRETPWVLEAQNENQQKKN
                     IALLFDVVKMSRELKSAVDKFGQMQSETGGFPWFKGGRDDRYITQYIISGIGHLKKLG
                     AVPANERSVLDKIARAGIAYLDKELKSEYDRRPKKTGPDISAEQIQYLYMRSFFPETG
                     LPGTVFPAMNYYRKQAIQYWPQQNMYLRGMIALFLYRTGDRKTAGDILHSLKENATRS
                     EDQGIYWKSVKPGYYWQESPVETQALLIEAFNEIKPEPDFTDQMKYWLLRQKHTGHWS
                     NTKSTADACYAILLNGTNWLASSPIADIKLGDAVLPPADEKAEAGTGYMKSVIPGKEV
                     QPEMGKIRVTVHHEHNATAAPSWGALYWQYFENLDKIPAAQSPLSVSKSLFIEKNTKN
                     GPVLEPVSAGNILRPGDKLKMRIIIKSDRDMQYVHLKDMRAACLEPVNVLSGYHWQGN
                     LGYYESTKDESSSFFFDYLPRGSFVFEYPLFVTTAGTYSNGITTLECMYAPEFSAHTE
                     GVRIRVENGK"
     gene            3305..4366
                     /locus_tag="VGM24_10405"
     CDS             3305..4366
                     /locus_tag="VGM24_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4155832.1"
                     /translation="MKEIDALIVGQGLAGTWLSWWLEQGGLSYRILDDADPLSASRHA
                     AGLINPVTGRRLVKTWMIDELMPFAWKCYSEMGSFLKTDLISETTVVDFFPTVQMLQA
                     FQKRCAEEESYLQTGEPDVRFDSWFRYDLGWGAIRPCFLVQTPRVLTAWRNHLEEKGY
                     LEEGLFDWDKLMPGGPSVEYAGMHYRYIVFCDGKQAARNRLFEKLPFALNKGEGLLVE
                     IDEIPRQTVFKKGMSLVPWENNIYWVGSSYEWEFADERPTEIFRSATESWLRNFLRVP
                     FKVVGHFAALRPATLERRPFAGFHPGYPRIGILNGLGTKGCSLAPYFAAQLAGKMISG
                     RDIDPLADIKRFERILMKA"
     gene            4464..>4996
                     /locus_tag="VGM24_10410"
     CDS             4464..>4996
                     /locus_tag="VGM24_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155833.1"
                     /translation="MHKKKLYFSRTIQATAIIVFLVFLLTQCVSKNKSPAYIVQNPDY
                     SLFAGSVTCVRCHKDIYDTHIHTAHFLTSQPASADHILGSFDAGKNRFSFADHKAVIM
                     EKRNDGFYQVEYDSGVQKTARRFDISIGSGTKGQSYLYWMNDQLFQLPIGYYTPTGQW
                     SNSPGYPDQVIFNRQVGS"
BASE COUNT         1335 a         1163 c         1340 g         1158 t
ORIGIN      
        1 aagggaaaat ggttttggag aaaacagaca gcacccggag caatggttta tttacgcttg
       61 atccggaaag catttcccgg gcccctgccg gctggtatct ggttgagttc cgtgcgaaag
      121 atccgaacgg ggaaaccgta acagataaaa gatatattga acttttccgg aaggaagaga
      181 gaaaagcgga ctatccaacg tataacctgg ttgcttcgga cgaccagacc ggcgagcccg
      241 gaaaagagct gaccattaaa accgggacct ccgcaacggg tatatatgtg atccgggcaa
      301 aggaaggtct gcaggatagc ctgacggatt attcctttta ccagctcaac aatgaaatca
      361 aatcaaccac tttaaaagtg gaagaaaagg accggggcgg ttttgcggtg aatgatgtat
      421 ttattaaaaa caaccgctgg tatagcagca cccacaccat tcatataccc tggacgaata
      481 aggaattgca ggtgagttat gaaacctgga ggaacaagct gcttccgggc agcaccgaag
      541 aatggaaggt aaagatcagc ggatataaaa gaaacaaagt tgccgctgaa gtgctgacgg
      601 ccatgtatga tgcgtccctg gaccagttta agaaacaggc ctggagcatc cctgatcttt
      661 atccggaatt ttccggagag accagctgga gcgggatcag tgattttgat gaccagggat
      721 ccatgggaag gccggtgacg gaataccaga aagaaaattt ctacaaacaa tacgatcagc
      781 gggtctcatc cgaagggcgg gggatcatgg tacgggagga gttttctccc gcggctccgg
      841 tccaggccaa gtactccggt gatttcgatt tatctccaaa gaaaatgacc ataggcaaag
      901 aggctgagcc ggctaaacaa ttgccggagg cgccgaatcc gggcgaacag atccgtaaaa
      961 atttcaatga aaccgctttc ttcttccccg acctgaaaac agatccggag ggtgatgtca
     1021 gcatttcctt taccatcccc gaagcattga cgcagtggaa gtggatgatc ttcgcgcata
     1081 cccgcgacct ggctttcggc tattccgaaa aaacggtggt tacgcaaaag gacctgatgc
     1141 tgcagcccaa tctgccgcgc tttttcaggg aaggagatac aatgagcctg ccggtgaaaa
     1201 tttcgaatct ttccaccctg gcaatgaacg ggacggttag tctggactgg ctggatgccg
     1261 ccgataacag caaccagaat caagcgctgc aaaaaatagc ccggcagcct ttccgcgtag
     1321 aggccgggca aagcacggtt gtttattttc cggctgttat tccgaaggat ttcgggcaac
     1381 cggtggtata ccggctacag gcaaagacca attcaggaaa ggcaggtgac ggggaatcgg
     1441 gtgtcatacc ggtgttgagc aaccgaatac tcgttacgga aacatttccc ctgaatatgg
     1501 ccgggcagga caataaggaa ttttcctggg agagattgct gaacagcaga acaagcccta
     1561 cgcttcaaaa ccagtcgctc accgtggaat atacgtccaa tcccgcctgg tatgcggttc
     1621 agtcacttcc ttacctgatg gaatttccct atgaatgtgc ggagcaaagc tttaaccgct
     1681 tctatgcaaa cgctctcgca gcccggatca taaaccatgc gccggttatc cgcgccatct
     1741 ttgagaaatg gaaaaacagc gacagttccg cgctgttgag taatcttcaa aagaatgaag
     1801 agctgaaaac cgtattgctg cgggagacgc cctgggtact cgaagcgcag aatgaaaatc
     1861 agcaaaagaa aaatatcgcc ctcttatttg atgtggtaaa aatgagccgg gaattgaaat
     1921 ccgccgtcga taaattcggg caaatgcaat cggagaccgg cggcttcccc tggttcaaag
     1981 gaggacggga cgaccggtat attacccagt atatcatttc gggaatcggt cacctgaaga
     2041 aattaggcgc cgttcccgcg aatgaacgtt ctgtgctgga taagatagcg cgtgccggaa
     2101 tcgcttacct ggacaaggaa ctgaaatccg aatacgaccg tcggccgaaa aaaacgggtc
     2161 ccgatatttc ggccgaacag atccagtatt tgtatatgcg cagtttcttt cccgaaaccg
     2221 gattacccgg aactgtattc ccggcaatga attattaccg caaacaggcg atacaatact
     2281 ggccgcagca aaatatgtat ttacggggaa tgattgcgct ttttctatac cggaccggag
     2341 accgaaaaac ggccggggat attcttcatt cattgaaaga aaacgcaacc cgttcagagg
     2401 accagggcat ttactggaaa tccgttaagc ccggatatta ttggcaggaa tcgccggtgg
     2461 aaacccaggc cctgctcatc gaagcattta acgagatcaa accggagccg gattttaccg
     2521 atcagatgaa atactggctg ctccggcaaa agcacaccgg gcattggagc aacaccaaat
     2581 cgactgctga tgcctgttat gccattctgc tgaacggaac caactggctg gcttcgtccc
     2641 cgattgccga cataaagctg ggcgacgcag tattgcctcc ggcagatgaa aaagccgaag
     2701 cgggaacggg gtatatgaag agcgtcattc ccggtaagga agttcagccg gaaatgggaa
     2761 agatccgggt gaccgttcat catgaacaca atgccacggc agcgccgtcc tggggcgcgc
     2821 tgtactggca gtattttgaa aacctggata agataccggc ggcacagtct cctttgtcgg
     2881 ttagcaaatc cttgtttatc gagaagaata cgaaaaatgg cccggtgctg gaaccggtat
     2941 ctgccggaaa tatattgaga ccgggagata aattgaaaat gcggatcatc attaaaagcg
     3001 atcgcgatat gcaatatgtg catctgaagg atatgcgggc agcctgtctt gaaccggtga
     3061 atgtgctgag tggttatcac tggcagggga acctgggtta ttacgaaagt accaaagatg
     3121 aaagcagcag tttctttttc gattatttgc ccaggggcag ttttgttttt gaatatcctt
     3181 tatttgtaac cactgcggga acctatagca atggcattac gacgctggaa tgtatgtatg
     3241 cgccggaatt ctcggcgcat accgaagggg tccggatccg cgtggagaac gggaaataat
     3301 tattatgaag gaaatagatg cactgatcgt tgggcaggga ctggcgggca cctggttaag
     3361 ctggtggctg gagcagggcg gcctttccta ccgcatcctg gatgacgcgg atcccttgtc
     3421 ggcttccaga cacgcggccg ggctgatcaa tccggttacc ggaagaaggc tggtcaaaac
     3481 ctggatgatt gatgagctga tgcctttcgc atggaagtgt tactcagaaa tggggtcttt
     3541 tcttaagacg gacctgatct ccgaaacgac ggtcgtggat ttctttccca cggttcagat
     3601 gcttcaggca tttcagaaac gctgcgcgga agaggagtcc tacctccaaa cgggcgaacc
     3661 ggatgtccgg tttgattcct ggttccgcta cgatctggga tggggagcta tccggccctg
     3721 ttttctggtg cagactcccc gggtgctgac cgcctggcgg aatcatctgg aggaaaaagg
     3781 gtatctggaa gaaggcttgt ttgattggga caaactgatg cccggcgggc catcggttga
     3841 atatgcgggt atgcattacc ggtatattgt tttttgtgac ggaaaacagg ctgcccgaaa
     3901 caggcttttt gaaaaattgc ctttcgctct caataaagga gaaggattac tagttgagat
     3961 agatgaaatt ccccggcaaa ccgtttttaa aaaagggatg agcctggtgc cctgggagaa
     4021 caatatttat tgggtcggat cttcttacga atgggaattt gccgatgagc ggccgacgga
     4081 aattttccgg tctgccacag aaagctggct aaggaatttc cttagggttc cctttaaagt
     4141 tgtcggccat tttgcggcct tgcgtccggc tacgctcgaa agaaggcctt ttgcgggctt
     4201 tcatcccgga tatccccgga tcggaatact caatgggctg ggtaccaagg gttgttccct
     4261 tgctccttat tttgccgccc agctggccgg gaaaatgatt tccggccggg acattgaccc
     4321 cctggccgac ataaagcgat ttgaacggat tttgatgaaa gcctgatatc cgggaaatca
     4381 gggtgccatg gaagatcacg atctccccat ttaatataaa agccgaaaga ttttttatct
     4441 tgctggtaat ctgattgccg gtcatgcaca aaaagaagct ctatttttcc agaacaatcc
     4501 aggcaacagc aatcatcgtt tttctggttt ttttacttac gcaatgcgtc tcgaagaata
     4561 agagcccggc ttatattgtt cagaatccgg attatagtct gtttgcggga tcggtgacct
     4621 gcgttcgttg tcataaggat atttacgaca cgcatattca taccgcccac ttcctcacgt
     4681 cccagccggc ttccgcggat catatcctgg gtagtttcga tgccggaaag aatcgttttt
     4741 catttgctga tcacaaggcc gtgatcatgg aaaaaagaaa tgatggattc tatcaggtgg
     4801 aatacgacag cggggttcag aaaacggccc ggcgctttga tatcagcatc ggctcgggca
     4861 ccaaggggca gtcttacctg tactggatga acgaccagct ttttcaactg ccaatcgggt
     4921 actatacgcc aaccggccag tggtctaaca gcccgggata tcccgatcag gtcattttta
     4981 accgtcaggt gggttc
//
LOCUS       DASYCU010000519         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3301941, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000519 DASYCU010000000
VERSION     DASYCU010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3301941"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGM24_10415"
     CDS             <1..373
                     /locus_tag="VGM24_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY4155834.1"
                     /translation="LHRVARNKIIDRYRRIRPESLEDLAYENEEGEMNFKEILLGDPG
                     NPEIEYLKNLFWEQLNEGLMELPSEQREIFILNELEGRTFAEISAETGENIKTLISRK
                     GYAVKHLRKRLRVLYEEFINY"
     gene            384..701
                     /locus_tag="VGM24_10420"
     CDS             384..701
                     /locus_tag="VGM24_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155835.1"
                     /translation="MERKRNRFYFIPIIIALIFLMGWVVMLLWNAILPDLINVRSITY
                     EQALGLLILCKILFGSYRPGPSRGFGKGGPHWRSKLMDLSPEERERFKQEWRNRAAGK
                     DQP"
     gene            852..1127
                     /locus_tag="VGM24_10425"
     CDS             852..1127
                     /locus_tag="VGM24_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155836.1"
                     /translation="MRFVIRAILTGLFIGALFYFVPVGFPILIFLLFLLFFFRFFGGR
                     RWRRGGYGGCGNYSREEGSPPENTPIDGYGSYRAGGPDTAIRRINIQ"
     gene            1146..1364
                     /locus_tag="VGM24_10430"
     CDS             1146..1364
                     /locus_tag="VGM24_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155837.1"
                     /translation="MRRGNFLTVIGVALITYFALTAFLGRRSWRGWNHRHYGYYNQCE
                     RADSGARYDRNEHRKPEPQPLTDSSLIQ"
     gene            1449..1757
                     /locus_tag="VGM24_10435"
     CDS             1449..1757
                     /locus_tag="VGM24_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY4155838.1"
                     /translation="MNIEETENEFIINLFAPALKKENISIFTQNDILSVRYKAEEGDS
                     SLRFTRKEYRREEISRSFELKGKVDIEKIRASYTEGVLKIELPKTEAAKRPMQDIPVS
                     "
     gene            complement(1790..>2749)
                     /gene="ligD"
                     /locus_tag="VGM24_10440"
     CDS             complement(1790..>2749)
                     /gene="ligD"
                     /locus_tag="VGM24_10440"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY4155839.1"
                     /translation="LKKAKNGERKTLLNPYEETQVRELNGHTLRFSHLSKIYWPKAGY
                     TKRDLLNYYYQAAPYILPYLKNRPQSLNRFPNGIEGKSFYQKDVTATAPDWVRKFPYH
                     TSTGEDKNFLVVEEESSLLWMANLGAIEMNPWNSTIQKPGYPDWCIIDIDPTEKNSFE
                     QVIRTAQVTKTVLDELGIPGYCKTSGSTGMHIYIPLAARYTYDQCQLLGKWIASRVHQ
                     ELPAFTSIERMTNKRRGKIYVDYLQNRPQATLAAPYAVRPKPGATVSMPLHWEEVKKG
                     LKMSDFTLQNAISRIRSEGDLFKPVLGKGINLKKVTGTMHEMD"
BASE COUNT          688 a          655 c          705 g          701 t
ORIGIN      
        1 gcttcaccgc gttgccagaa ataagatcat tgaccgctac cggaggatca gaccggaatc
       61 cctggaagat cttgcctatg aaaatgagga gggcgaaatg aattttaaag agatcctcct
      121 gggggatccc ggcaatccgg aaatagaata ccttaaaaat ctattctggg aacagcttaa
      181 cgaagggtta atggaattac cttcggaaca aagggagata ttcattctaa atgaactgga
      241 aggcaggact tttgcagaaa tatcggcgga aacaggagaa aatatcaaaa ctttgatctc
      301 ccgtaaagga tatgccgtaa agcatttgcg caaacggctg cgggtcctct atgaggagtt
      361 tatcaattat taaaattagt ggcatggaaa ggaaaagaaa ccgtttttat ttcatcccta
      421 tcattattgc cctgatcttc ctgatgggat gggtcgttat gctcctgtgg aatgccatcc
      481 tgccggatct gatcaatgtg cgcagcatta cgtacgagca ggcgctcgga ctgctcatct
      541 tatgcaagat ccttttcgga tcctaccggc ccggaccatc ccgcggattt gggaaaggag
      601 gcccgcactg gcgttcaaaa ctcatggacc tttctccgga ggaaagggaa cgatttaaac
      661 aggaatggcg gaaccgggct gccgggaaag accagccata ggatcttaaa ttccaaacta
      721 tagtaaggac ccgccacggt ccttttttta tgtccgggat atttttccgg aaaatcagga
      781 ccgggttaaa gtaaaaacga gcgtcatccg tctaccggag agacactatt attcaataaa
      841 aaataaaagc tatgcgattt gtcataaggg ctattctgac cgggttattc atcggcgcac
      901 tattttactt tgtgcccgtt ggtttcccca ttttgatatt tctccttttc ctgctctttt
      961 ttttccggtt tttcggagga agacgatggc ggagaggcgg atatggcggc tgcgggaact
     1021 attcccggga agaaggttct cctccggaga acactcccat cgacggctac ggctcttacc
     1081 gtgccggcgg cccggatacc gccataagaa gaataaacat tcagtaagct ttaaaaaatc
     1141 aaaccatgag acgcggaaat tttttgacgg ttatcggggt ggccctcata acctattttg
     1201 ccctcactgc ttttctgggc cgcaggtcct ggcggggatg gaaccaccgg cattacggtt
     1261 attataatca atgtgagcgg gcggatagcg gcgcccggta tgaccggaat gagcatcgta
     1321 agccggaacc acaaccgctg accgattcat cccttattca ataatttaaa taaaataaaa
     1381 tgcaatacag atatcaccat tgcggtccgg gaggctggaa gcctcgcgga ttttcaaatt
     1441 cggcgcccgt gaacatagag gaaacagaaa atgaattcat cattaacctg tttgccccgg
     1501 ccttgaaaaa ggaaaacatt tccatcttta cacaaaacga tatactctcc gtccggtata
     1561 aagcggagga gggcgattcc tcgctgcggt tcacccggaa ggaataccgg cgggaagaaa
     1621 tttcccggtc ttttgaattg aaaggcaaag tggatatcga aaagatcagg gcttcttata
     1681 ccgaaggcgt tcttaaaatt gaattgccaa aaacggaagc ggcaaaaaga ccgatgcagg
     1741 acatccccgt gagttagccg cgaagacggt tccgggcaga ttccccgcat cagtccattt
     1801 catgcatggt cccggtcact tttttcaggt tgatcccttt gcctaaaacc ggcttaaaga
     1861 gatctccttc ggaccggatc cgggaaatgg cattctgcaa ggtgaaatcg ctcatcttca
     1921 gtcccttttt tacttcttcc cagtgcaggg gcatggaaac ggtggcgccg ggtttcggcc
     1981 ggacggcata cggcgcggca agcgttgcct ggggccggtt ctgaagatag tccacgtaaa
     2041 tttttcccct acgtttattg gtcattcgtt cgatactggt gaaggcagga agttcctggt
     2101 gcacgcggct ggcaatccat tttcccagta actggcactg gtcgtaagtg taccgggcag
     2161 cgagcggaat gtaaatatgc attccggtgg acccggaagt tttgcaatag ccggggatcc
     2221 cgagctcatc caaaaccgtt ttggtcacct gcgcggtccg gatcacctgt tcgaaactgt
     2281 ttttttcggt cggatcgata tcgataatgc accagtccgg atagcccggc ttctggatgg
     2341 tgctgttcca gggattcatt tcgatggcgc ccaggttggc catccaaagc aggctcgact
     2401 cctcttccac taccaggaaa ttcttatctt ctcccgtgct ggtatgatag ggaaatttcc
     2461 gcacccagtc cggcgccgtg gccgtaacgt ccttctgata aaaacttttt ccttcgatgc
     2521 cgttgggaaa ccggttcagg gattgcggcc ggttcttcag gtaaggcaat atatatgggg
     2581 ccgcctggta ataataattc agcaggtcgc gcttggtata gcccgcttta ggccagtaga
     2641 tcttgctcag atggctgaaa cggagcgtgt gcccgttcag ttcccggacc tgtgtttctt
     2701 cgtacgggtt gagcagggtt ttacgttccc cgtttttggc ttttttaag
//
LOCUS       DASYCU010000520         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2430916, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000520 DASYCU010000000
VERSION     DASYCU010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430916"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VGM24_10445"
     CDS             complement(<1..560)
                     /locus_tag="VGM24_10445"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY4155840.1"
                     /translation="MRPGTASAILVLLFSWHSLRLTAQSLPDATRHKIDALFKYWDNY
                     NSPGCAIGIIRNDSLIYARGYGMANLEYGIRIEPETIFHMASVSKQFTAYSIVLLAEK
                     DKLNLDDDIHKYLPWFPDLKVKISIRNLLNHVSGIRDQWNLLAISGTRLDDVITQDQI
                     IKVLSRQQALNFNPGEQYSYSNSGFT"
     gene            756..1478
                     /locus_tag="VGM24_10450"
     CDS             756..1478
                     /locus_tag="VGM24_10450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4155841.1"
                     /translation="MRFLPYLRYFFYIAWHWNPILALFTIYYELKGEKKYHIHTIGED
                     ELNSLKERGIDISHAHIYMPVNYFVLERLMNEIAHYDHNKTFLDLGCGKGRVMIVAAE
                     FGFEEITGIDLSKEFCEEAEAATAAYIRKNERMRFTVINGDALYFEIPGDIGTIFLFN
                     PFDEVILEGVVSNILKSQQLSPRTIRVLYANPQHESVLLDHGFVLIYHLKKWNYFEGV
                     ILEKKAGDLPAGRIPTPLLSPG"
     gene            complement(1453..2385)
                     /locus_tag="VGM24_10455"
     CDS             complement(1453..2385)
                     /locus_tag="VGM24_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4155842.1"
                     /translation="MKVSPNIICIASVVFFSLARSGYGQKTHSGEKQEGAIIAAGIKP
                     VLIDSRFSFTEGPAVDKQGNVFFTDQPNNRIWEYDINGKLKLFLDSAGRSNGMYFDSK
                     GNLITCADEKDQLWSIDKNKKIRILLKDYRGHRLNGPNDLWISPSGDIYLTDPYFQRD
                     YWTRKMADPALGGEKLYCLRHNQKELILVESETVKPNGLVGAPDGKWLYVADMGLGKT
                     FRYGINPDGTLKNKQLFVNQASDGMTIDNRGNIYLTGDGVSVYNPAGKKIAQIDIPEK
                     WTANICFYGKEKNILFITASKSIYLLPTRVKGVE"
     gene            complement(2391..>3161)
                     /locus_tag="VGM24_10460"
     CDS             complement(2391..>3161)
                     /locus_tag="VGM24_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY4155843.1"
                     /translation="MDNLVNQSRLMGTKPVIYNVCNFSKPPPGQPALISFDDVTTMFH
                     EFGHALHGLFASQEYPSLSGTSTARDFVEFPSQFNEHWASDPKVFDHYAVHYRTSSPM
                     PAALVAKIKKSAKFNQGYALTELLAAADLDLQWHSLGADDIVQNADSFQVAALHATHL
                     DLPQVPSRYRSSYFSHIWSGGYAAGYYAYLWTQMLDDDAFSWFKEHGGLTRENGQRFR
                     DMILSRGNTQDLGEMFRNFRGHDPDIRPMLENRGMTVK"
BASE COUNT          762 a          781 c          784 g          834 t
ORIGIN      
        1 attgtaaatc cgctgttcga atagctgtat tgttcaccgg gattaaaatt aagggcctgc
       61 tgccggctca ggacctttat gatctggtcc tgcgtgatca catcgtccag cctggtgccg
      121 gaaatggcca gcaggttcca ctggtccctg atcccgctca cgtggtttaa aagattccgg
      181 atgctgatct tcactttcag atcgggaaac cagggaaggt atttgtggat gtcatcgtcc
      241 aggttaagtt tatccttttc cgccagcagc acgatggaat aggccgtaaa ctgtttggat
      301 acggaagcca tgtgaaaaat cgtttccggc tcaatgcgga ttccatattc caggttagcc
      361 ataccatatc cccgggcata aataagagaa tcatttctga tgatgcctat ggcgcatccc
      421 gggctgttgt aattatccca atatttaaaa agggcgtcaa ttttatgcct ggtggcatcc
      481 ggcagggatt gtgcagttag tctaaggcta tgccatgaaa ataacaggac cagaatggcg
      541 gatgctgtac ccggacgcat aaggattggc tttccttaaa tttacggagt ctgattcatt
      601 ttagccaatt ctatctccgc catcaccgga aaatgcccgg atgattccct tgcagtgtat
      661 tttgaggggt tcgggtcttt tggaggatcc gccagccggt aaccattatc tttgcaccag
      721 ctgacaacaa acaccgggga gctaaataac gcgttatgcg gttcctgcct tacttacgtt
      781 atttcttcta tattgcctgg cactggaatc ccatccttgc tttatttacc atttattacg
      841 agctcaaagg ggaaaagaaa tatcatattc acacgatcgg ggaagatgaa ttgaacagcc
      901 tgaaagaaag gggcatcgat atctcgcacg cccatattta tatgcccgtc aattattttg
      961 tgctggaacg cctgatgaac gagatcgcgc attatgacca caacaaaacc ttcctggacc
     1021 tgggctgcgg caagggaagg gtgatgatcg tggcggcaga atttggcttt gaggaaatta
     1081 ccggaatcga tctttcaaaa gaattctgtg aggaagcgga ggcggcaacg gcggcttata
     1141 tccggaagaa tgagcgtatg cgctttaccg ttattaacgg ggatgccctt tattttgaaa
     1201 tccccggcga tatcggcacc atttttcttt ttaatccctt tgatgaggtg atcctggaag
     1261 gggttgtttc caatatctta aaaagccagc agttatctcc gcgcacgatc cgcgttctgt
     1321 acgccaatcc gcagcacgag tccgtgctgc tggatcatgg atttgtcctg atctatcacc
     1381 tgaagaaatg gaattatttt gaaggcgtta tcctggaaaa aaaagcgggc gatctcccgg
     1441 ccggaagaat ccctactcca ctcctttcac ccgggtaggc aagagataaa tggatttaga
     1501 cgccgttata aataaaatat tcttttcttt cccgtaaaaa caaatattcg cggtccactt
     1561 ttccggaata tcgatctgcg caattttttt tccggccggg ttgtacacgg agaccccatc
     1621 gccggtaagg taaatattcc cgcggttgtc gatggtcatc ccgtcggatg cctgattgac
     1681 aaagagctgt ttgtttttca gggtcccgtc cggatttatt ccataccgga acgtttttcc
     1741 caaacccata tccgccacgt agagccattt gccgtccggc gcacccacga ggccgttagg
     1801 ctttaccgtt tcactttcca ccaggatcag ttctttttga ttatgccgga ggcaatacag
     1861 cttttcaccc cccagcgccg gatcggccat cttcctcgtc cagtaatccc gctgaaaata
     1921 ggggtccgta agatagatat ctccggacgg cgagatccag agatcgttgg gaccgttcaa
     1981 ccggtgtccg cggtaatctt tcagcaggat cctgattttt ttgttcttat caatggacca
     2041 aagctggtcc ttctcatcgg cgcaggtgat cagatttcct tttgaatcga aatacatccc
     2101 gttggaccgg ccggcgctgt ccagaaaaag cttgagcttt ccgttaatat cgtattccca
     2161 gatcctgttg ttcggctggt ctgtaaaaaa tacattcccc tgtttatcca cggcggggcc
     2221 ttcggtaaag ctgaagcggc tgtcgatcaa tacgggcttg atccccgccg cgatgattgc
     2281 tccctcctgc ttttcgccgg aatgggtctt ctgaccgtag ccggatcttg ccaatgaaaa
     2341 aaatacaacg gaagcaatgc agattatgtt ggggctgact ttcatcaaga tcatttaacg
     2401 gtcattccgc ggttttccag catcggccgg atatcgggat cgtgtccgcg aaaattcctg
     2461 aacatctcac cgaggtcctg ggtgttgccg cgggacagga tcatgtcccg gaaacgctgg
     2521 ccgttttcgc gggtcagtcc tccgtgctcc ttaaaccagg agaaggcatc gtcatccagc
     2581 atttgcgtcc acagataagc ataataaccg gcggcataac cgcctgacca gatatgggaa
     2641 aaatagctgc tgcgatagcg gctgggcacc tgcggaagat ccagatgggt tgcgtgcaga
     2701 gcggcaacct ggaatgaatc cgcattctgc acgatatcat ccgcacccag ggaatgccac
     2761 tgcaggtcga gatcggccgc ggcgagcaat tccgtgaggg catatccctg gttgaatttt
     2821 gccgattttt ttattttagc caccaaagct gccggcatag gagaactcgt ccggtaatga
     2881 acggcataat gatcaaacac tttcggatcc gatgcccagt gttcattgaa ctgcgacgga
     2941 aattcaacaa aatcccttgc ggtgctggtt ccggaaagac tgggatattc ctggctggcg
     3001 aacagtccgt gcagggcatg accgaattca tgaaacatgg tggtaacatc atcgaaactg
     3061 atcagcgcgg gctgtccggg cggcggtttg gagaaattac agacattgta aataaccggt
     3121 ttggttccca tcagcctcga ctggttaacc agattatcca t
//
LOCUS       DASYCU010000521         5599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1270540, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000521 DASYCU010000000
VERSION     DASYCU010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270540"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..965
                     /locus_tag="VGM24_10465"
     CDS             168..965
                     /locus_tag="VGM24_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEY4155844.1"
                     /translation="MPHTHYPLRTVNVTRYVTPLREGGSLPAIAEADDGFLYVLKFRG
                     AGQGIRALIADLIGAEIARALGLKVPELVFANLDTAFGRTEPDEEIQDLLKGSVGLNL
                     GLHYLSRAISYDPAVTALDADLASRIVWLDCLITNVDRTARNTNMLVWKNELWLIDHG
                     ASLYFHHSWQGWKEQATKPFLQVKDHVLLPQASALGKIDRECRSILPPELIQSIVSLI
                     PEDWLATAPQEASPAEHRKVYADFLNLRIAHSEIFVNEAQHARKSLV"
     gene            943..1329
                     /locus_tag="VGM24_10470"
     CDS             943..1329
                     /locus_tag="VGM24_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEY4155845.1"
                     /translation="MPENHLFEYAVIRVVPRVEREEFLNAGVILYCPDQKFLQARFEL
                     NEPRLRAFCKLIDPDELREHLLAIGKICQGEKEAGPIGQLDMASRFRWLTAKRSTVVQ
                     TSPVHPGLCEDAGEMLSRLFSQLVVF"
     gene            complement(1353..1964)
                     /locus_tag="VGM24_10475"
     CDS             complement(1353..1964)
                     /locus_tag="VGM24_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEY4155846.1"
                     /translation="MKILSVKSVSFFAAFLIFSLGARSQKTMTRDQAIQWMKSGVWKH
                     GLQKKPHASIDPQEFAEQYQRNPAIWDKAFAFLNRKDLDTLSPGNYPLDGDRVYGIVS
                     VYDSKDMDKTKWESHRKYIDLQYVIKGKEKIGVAPVSKAKVTEPYDESKDIAHYESDG
                     KYYLAQPGVFFLFFPQETHRPGIKVEGSVPVRKLVIKIRVAGT"
     regulatory      complement(2131..2228)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(2263..3690)
                     /locus_tag="VGM24_10480"
     CDS             complement(2263..3690)
                     /locus_tag="VGM24_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4155847.1"
                     /translation="MAQLQRKLGLWTSVSIVVGTVIGSSIYMKPATMAGQLGSPLLLL
                     LVWIVAGVVSLFGGMINAEVGCVLPETGGQYVYFRHMYGNFFAYLYGWASFIVINSAS
                     IAGISFVFAEYSAYFFPLPRFPAAVEQSIPLVIPGIGRFYLLENIGVKGLAIGLILAF
                     TWMNTRSVKISGMVQVFLSVLKVAALILLVGAIFFSGKGDFLNLTRGSAGFDHSFWSV
                     FPAFIAATTGALAAYDGWNNLGFVAGEIRDPQKNIPRGLMIGIGLCILLYVFTTEAYL
                     YVLPIDVMKNSTLLASDALFKVLGVAGAGFAAFLVVISTAGSTNGNIMPCARVIYAMG
                     EEKNFFSFTGKVNAKHQTPANALWLQAIWSSLLVLIGSFDMLMDMFVFITWIYYGFGA
                     YGIFILRKKMPLAERPYKLRGYPYLPVIFILFAVIYVVVTLYNDTSAYLSGRTHIINS
                     LFGLLLTAIGIPLYGYFKKRGPSGG"
     gene            complement(3695..4801)
                     /locus_tag="VGM24_10485"
     CDS             complement(3695..4801)
                     /locus_tag="VGM24_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY4155848.1"
                     /translation="MNFKTAMTDFSVDPDIATAKTLNAEFYTDKELFEQCREKIFAPS
                     WQFIGTEERVNRPGDVYPFVFLENCLDEPLLLTRDKTEKLNLLSNVCTHRGNIVSDRP
                     GNLSQLRCRYHGRVFNLDGRFVSMPEFKEVRNFPTPQDDLPRLDLFSWSRWLFASLQQ
                     KYPPELFLKDMMGRVSWMPLNEFSFRPDLSRVFHVKAHWALYCENYLEGFHIPFVHAG
                     LNAVIDFGEYATELFFPYSSLQLGLAKNASDCFDLPSSSPDYGKKVAAYYFWVFPNMM
                     FNFYPWGLSVNVVEPVSVEQCRVRFFSYVWDASRLNSGAGAGLDRVEQEDEEIVEKVQ
                     EGIRSRFYKQGRYSVTREQGIHHFHRILAEFLNK"
     gene            4885..>5599
                     /locus_tag="VGM24_10490"
     CDS             4885..>5599
                     /locus_tag="VGM24_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEY4155849.1"
                     /translation="MRYCILLFCLALFTNTVTRAQKRKNPQPAPPGRSFYTEKPDDPE
                     AVYFLPGNFQVKADGSADVSDALQEAINEVKNQHNFGILFIPSGKYLIRKTIYIPRAV
                     RVIGYGNTRPEIILARNSPGFQTADSSDKGRAKYMFWFTSSVVKPGEAVNDAGAGTFY
                     SALSNVNLTIEDGNPAAVALRAHFAQHSFISHVDIHIGNGRAGLFDVGNEMEDTRFFG
                     GDYGIYTTKPSPGWQFMMLD"
BASE COUNT         1469 a         1477 c         1378 g         1275 t
ORIGIN      
        1 aagacagtct tccggaaaga atgctgaagt atttacaggg caagacctgc tttcacataa
       61 aaaaagaaga cccgccgctc atgcgttcca tcgaaaaggc gctggaaacc ggttatgcct
      121 attataaatc gaagggctgg caataaatta aattccgcaa gcaatccatg ccgcataccc
      181 attacccgct cagaacggtg aatgtaaccc gctatgtcac gcccctgcgc gaaggcggat
      241 cgctgccggc catagccgaa gcggacgatg gtttcctgta cgtgcttaag ttccgtgggg
      301 cgggacaggg aatccgggcc ctgatcgccg acctgatcgg tgcagaaatt gcccgcgcac
      361 tcgggttgaa ggtgccggaa ctggtgtttg ccaacctgga tacggccttc ggtcgcacgg
      421 aaccggatga ggaaatccag gatctgctga aagggagtgt cgggctcaac ctcggcctcc
      481 actatctttc ccgcgccatt tcctatgacc cggcggttac cgctcttgat gcggacctgg
      541 cctcccggat cgtatggctg gattgcctga tcaccaacgt cgaccgcacg gcgcggaata
      601 ccaatatgct ggtctggaaa aatgaattat ggctgatcga ccatggcgca tctctttatt
      661 ttcatcatag ctggcagggt tggaaagaac aggccacaaa gcctttcctg caggtgaaag
      721 atcatgtctt gttgccacag gcttcggctc tcggaaaaat tgaccgggaa tgccggtcca
      781 tactcccccc ggagcttatc caatccattg tatctttaat tccggaggat tggcttgcga
      841 cggcgcccca ggaagcgtcc ccggctgaac accggaaggt ttatgcagat tttctaaacc
      901 tgcggattgc ccactcggaa atatttgtaa acgaagcaca acatgccaga aaatcacttg
      961 tttgagtacg ccgtgatccg cgttgtgccc cgggttgaac gggaagaatt ccttaatgcc
     1021 ggcgtcattc tctattgtcc ggatcaaaag tttttacagg cccggtttga actcaatgaa
     1081 ccccgcctcc gggcattctg caaactgatc gatccggatg aactgagaga acacctgctg
     1141 gccatcggga aaatctgcca gggagaaaaa gaggccggcc cgatcggtca gctggacatg
     1201 gcttcccggt ttcgctggct cacggcaaaa cgcagcaccg tggtacagac ttccccggta
     1261 catcccgggc tctgtgaaga tgccggggag atgctcagcc ggctgttcag ccagctggtg
     1321 gttttttaga agtttttatt gcgcaccgcc catcaggtac cggccaccct gatctttatc
     1381 accagcttcc tcaccggcac cgaaccctcc acttttattc cggggcgatg ggtttcctgg
     1441 ggaaagaaca ggaagaaaac gccgggttgg gccaggtaat attttccgtc tgattcataa
     1501 tgggcgatgt cctttgattc atcgtaaggc tccgtcacct tggcctttga caccggcgct
     1561 acgcctattt tttcctttcc cttgatcacg tattgcagat cgatatattt ccggtgggat
     1621 tcccattttg ttttatccat atcttttgaa tcgtatacgc tcacgattcc gtaaacccgg
     1681 tccccgtcca gcggatagtt tccgggagaa agggtgtcca gatccttgcg gttaagaaat
     1741 gcaaacgctt tatcccagat cgccggattg cgctggtatt gctccgcaaa ttcctgcgga
     1801 tcaatggagg catgcggttt tttctgcaag ccatgtttcc agaccccgct cttcatccat
     1861 tggatggcct ggtctctggt catcgttttt tgtgaacggg cgcctaagga gaaaatcaaa
     1921 aaagcagcaa aaaaagaaac agactttaca gaaaggattt tcatgattta acttttaaga
     1981 gaaattaaat gtacaggaat acgaaagata ttcccaaccc ggggtgcaaa aaatagggga
     2041 accgggtaaa cttgtcccgg aaattttctc ccggtccttc ccgattcggt taaggtgggc
     2101 aaataaaaga ccccggtttt cgccggggcc tcaggtgttt cttattttcc cttcgctggt
     2161 attatccaga tcaggtgatg agggtatgtt ctcagatctg ttttacgaaa ccaccccttg
     2221 ttcagaaact tcctgactat atacccaaaa tatcgtgcca atctagccgc cggacgggcc
     2281 ccgtttttta aaatacccat acaggggaat gccgatggcg gtgagtaaaa ggccgaacag
     2341 ggaattaatg atatgggtcc tgccggagag gtaggcactg gtatcgttat acagggtcac
     2401 cactacatag atcaccgcaa ataaaataaa aatgaccggc aggtatgggt agcccctgag
     2461 cttatacggg cgttctgcca ggggcatttt tttccggaga ataaaaatac cataagctcc
     2521 gaaaccataa tagatccagg taataaatac aaacatatcc atcagcatat caaaggagcc
     2581 gataagcacc agcagggagg accagattgc ctgcagccat agcgcattgg ccggcgtctg
     2641 atgttttgca ttcacttttc ccgtgaagga gaaaaagttc ttttcttcgc ccatggcgta
     2701 aatgacccgg gcgcagggca taatattccc gttcgtggat ccggccgtgg aaataacgac
     2761 caggaaagcg gcgaatccgg cgccggccac acccaatacc ttgaataaag cgtctgaagc
     2821 cagcagggtg gaattcttca tcacgtcaat gggcaacacg tagagatagg cttccgtcgt
     2881 gaacacatat aagaggatac agagaccgat accgatcatc agtccccgcg gaatattttt
     2941 ttgcggatcc ctgatctccc cggcaacaaa cccgaggttg ttccagccat cataggcggc
     3001 gagcgcgccc gtggtcgccg caatgaatgc cggaaatacc gaccagaaag aatgatcaaa
     3061 ccctgccgat ccccgggtca gattcaggaa gtcccccttg ccggaaaaga aaatagcgcc
     3121 gacgagcagg atcagggccg ccactttgag caccgaaaga aagacctgaa ccataccgga
     3181 tatttttaca ctccgcgtgt tcatccaggt aaaagccagg atcaatccga tcgccaaccc
     3241 cttgacgcct atattttcga gcaggtaaaa tcgtccgatg ccgggaatga ccagtggaat
     3301 ggattgttca acagcagccg ggaaacgggg cagaggaaag aaataggcgg agtattccgc
     3361 aaatacgaaa gagatgccgg caatggatgc cgaattgatg acaataaagg aagcccagcc
     3421 atagagatag gcaaagaaat ttccgtacat gtgacggaaa taaacatatt gcccgccggt
     3481 ctccggcaaa acgcaaccca cttcggcatt gatcattccg ccgaataaag aaacaactcc
     3541 cgcaactatc cacaccagca ataacaataa gggcgagccc agctggcctg ccatggtagc
     3601 tggtttcata taaatactgc tgccgataac ggttccgacc acaatggata cggatgtcca
     3661 taaccctaat ttgcgttgaa gttgcgccat gcccttattt gttcaggaat tcagccagga
     3721 tgcggtgaaa atgatgtatg ccctgctccc gggtaaccga ataccgcccc tgtttataaa
     3781 accgggaacg tatgccttcc tgcaccttct ccacaatttc ctcatcttcc tgctccactc
     3841 tgtccagccc cgcaccggcg ccgctgttca agcgggaagc atcccataca taagagaaaa
     3901 agcggacccg gcattgctcc accgataccg gttccaccac attgaccgac aaaccccagg
     3961 gatagaaatt aaacatcata ttgggaaaga cccagaaata ataagccgcc actttctttc
     4021 cataatccgg agacgaggaa ggcaggtcaa aacagtctga cgcatttttt gcaaggccca
     4081 gctgaagact ggaataggga aagaataatt ccgtcgcata ttccccgaag tcgatcacgg
     4141 cattcagtcc ggcatgaaca aaaggaatat gaaagccttc caggtagttc tcgcaataca
     4201 gcgcccagtg cgcttttaca tggaatacgc gggacagatc cgggcggaag gagaattcat
     4261 tcaacggcat ccagctcacc cgccccatca tatctttcag gaaaagttcc ggcggatatt
     4321 tttgctgaag ggaagcgaac aaccaccggc tccaggaaaa cagatccagc cggggtaaat
     4381 catcctgcgg agtaggaaaa ttccggacct ctttaaactc gggcatggaa acaaatctcc
     4441 cgtcgaggtt aaaaaccctt ccgtgatacc ggcagcgcag ctgggacagg ttacccggtc
     4501 tatccgaaac tatatttccg cgatgggtgc aaacattgga caggagattt aatttctccg
     4561 ttttatcccg ggttaacaac aggggctcgt cgagacaatt ttccaggaag acaaaaggat
     4621 atacatcgcc cggacggttc accctttcct ccgttccgat gaactgccag gaaggcgcga
     4681 agattttttc cctgcattgt tcaaacagct ccttatccgt gtaaaattcc gcattgaggg
     4741 tttttgcggt cgcaatatca ggatctaccg aaaaatcagt catagcggtt ttgaagttca
     4801 tgaatgtacc gtttttccgc aagaaggaca tattcaggcg gtccaaagtt ttgtaatttg
     4861 cccgatattc caaccaaaga acttatgaga tactgcattt tactcttctg cctggcgtta
     4921 tttacaaata cggtaacccg ggcgcaaaaa agaaagaacc cccaaccggc tccgccaggc
     4981 cgctccttct ataccgaaaa gccggatgat ccggaagcgg tctatttcct gccggggaat
     5041 ttccaagtca aagcggacgg ttcagccgat gtttcggatg ccctgcagga ggcgatcaac
     5101 gaagtgaaga accagcataa tttcggcatc ctttttattc cttccggaaa atacctcatc
     5161 cgtaaaacga tctatattcc ccgcgcggtc cgcgtgatcg ggtacggcaa tacccgtccc
     5221 gagattatcc tggccaggaa ttctcccgga ttccaaaccg cggattcatc cgataaaggc
     5281 agggcgaaat acatgttctg gtttaccagc agcgtggtga aacccggaga agccgttaac
     5341 gacgccggag ccggtacgtt ctacagcgcc ctctccaacg ttaacctgac cattgaagac
     5401 ggcaacccgg cggcggttgc cctccgtgcg cacttcgccc agcatagttt tatttcgcat
     5461 gtggatattc atatcggaaa cggccgggcc ggtctgttcg atgtgggaaa tgaaatggaa
     5521 gacacccgtt tttttggcgg cgattacggc atctatacca cgaaaccttc cccgggatgg
     5581 cagttcatga tgctggaca
//
LOCUS       DASYCU010000522         6810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3158206, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000522 DASYCU010000000
VERSION     DASYCU010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3158206"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VGM24_10495"
     CDS             <1..493
                     /locus_tag="VGM24_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HEY4155850.1"
                     /translation="NYLGTHSDKHLLYCDWTNRDSLLVTHQQFDQDIDHAFEEMKNWN
                     ITKRRARYFLPPYEWYNDRIARWSREAGLKLVCFTPGTRSNADYTYPEMGNKYLSGAG
                     ILGSIRLFEKESPDGLNGFILLLHIGTDPRRKDKMYFLLPGLIRELKSKGYRFVTIPQ
                     LLR"
     gene            730..1812
                     /locus_tag="VGM24_10500"
     CDS             730..1812
                     /locus_tag="VGM24_10500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY4155851.1"
                     /translation="MYKVSFSNKDNSFHQELKRSVDQYFSTNHINKTGNWKLYIKTAI
                     LVPSAIAIYLLLLLVHFPVWLAIVLCTLLGFIFASIGFNIMHDACHGAYSKRTWVNTL
                     MGLSLNLLGGNAFIWKFKHNIIHHTYTNIDGLDDDIAKSPLMRQCPSQKWVKAHRFQH
                     FYVVLVYAISSFAWVTMMDFNKYFKRKIHNTSLQKMDLEEHFIFWASKAMYAIFYILI
                     PVHFVGWQAWGVGFACMHITMGLTLALVFQLAHVVEDAEFVFAPGIEPQKLEDEWAIH
                     QVRTTANFAPRNKFVSWFVGGLNFQIEHHLFPRVSHVHYPAISSIVKQICRNHHIHYN
                     EFRTMTGAVASHFRMMKMLGRKPVNY"
     gene            complement(1877..2311)
                     /locus_tag="VGM24_10505"
     CDS             complement(1877..2311)
                     /locus_tag="VGM24_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155852.1"
                     /translation="MKKIMMMLLSLGLVFGAAAQGHRGGTVHHGGSYHGGGYYVRPRV
                     SIGLGYYSPFGLYGYYPPFYMSPYGPYAYPYNGGWHNRQSKLAMNVQGIKNDYADRIY
                     SVRHDTRISHKERRKKVRELKRDRDKAVQDEIMNYYKSPSSQ"
     gene            complement(2427..4823)
                     /locus_tag="VGM24_10510"
     CDS             complement(2427..4823)
                     /locus_tag="VGM24_10510"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY4155853.1"
                     /translation="MKSLFSGKLMGLSLVLFFFNSLSFAFAQELPVNPDLLKGSWNAQ
                     WITCPDVPFRAYGVYHFRKTMDLRDKPARFIIHVSADNRYVLYVNGVEIGRGPARSSL
                     YNWNFGSFDIASHLQAGKNVIAALVWNMGEWAPVAQISNQTGFLLQGDSGAENLANSD
                     NSWKVLHDTAYTPCAGNMGAELHTYMVVGPGDQVRGKAYPWGWEQKDYNDGNWQMAKP
                     LFTPVVTSGYGTDNQWTLAPRSIPQMEEKMQRLAVVRRISGLAIDPAFISGTRPLNIP
                     AHTKLSILLDQSYETVAYPVIRLSQGKNAVVRLTYAEALFDQKGQKGNRNEIQDKTIK
                     GLYDIFYPDGQADRSFSPLWVRTYRYLQIDVQTEDEPLEIADVYGIYTGYPFVQKASF
                     SSDDGSLEAIWKTGWRTARLCAGETYFDCPYYEQLQYEGDTRIQSLISLYNTGDSRLM
                     QKAIDDFFNSRVPEGLTQGRYPSNRLQVIPPFSLFWVSMMYDYWMHCQDDRFLEKYLD
                     GADIVLKWFERRVDKKLGMLGPMKWWNFSDWNKAFPNGVPDGAVDGHSAVVSLQFAYT
                     LRQASAVFKYYGRNEAAAHYSALADLLAKNTYQHCFDLQKMEMANTPEKVSFSQHAGI
                     MGILADAIPENLQKQVLNHILYDSTLSQATFYYRFYLTRAMTRAGMGNLYYSQLTPWR
                     NMLKMGLTTFAEEPDPTRSDCHAWSASPNYDFLATLCGIMPDAPGFKKVLVSPQLGEL
                     KEIKGLMPIPAGSVEVSLKRKGTNGIEGVVTLPQNITGRFVWNGREIKLMGGHQRISL
                     "
     gene            complement(4900..5709)
                     /locus_tag="VGM24_10515"
     CDS             complement(4900..5709)
                     /locus_tag="VGM24_10515"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEY4155854.1"
                     /translation="MFHIVFQPRDAEVLTAAMDMDEALDGEIVLISDDYSLGPVTDLF
                     SEDGRALRRTWWAGILNPANSREEAGYDPADHTKLQQILERMREEEFDQIWIWVASNA
                     RDVSGYYWLISQLNEFAGRIYLLSLNNLPFINEKGLVFYPTGLAEIPPREFIKAKKLA
                     RPVTLSEFETDPDEWTKFGGENKNIRTLEGAKKLVQQEDDYYDAPLKKFVTAEFQKPS
                     RIINHFLSRSPVKTGETFLIWRLKQLLANAELEQQGELLRLAGGETVAEPG"
     gene            complement(5721..>6810)
                     /locus_tag="VGM24_10520"
     CDS             complement(5721..>6810)
                     /locus_tag="VGM24_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY4155855.1"
                     /translation="QAFREAFVKVVRDFYKKDYDAADIRQSLTAAVSVRVVEPVFESQ
                     TKTKLGSQYVVEGGASMKNFVHDFLARELDNYLHKNPSIADAMKKRIEQSEKERKELS
                     GIRKLANERAKKANLHNRKLRDCRVHYNEEPTGKDKELIAEKQKESTIFITEGDSASG
                     SITKVRNVETQAVFSLRGKPLNCFGLTKKVVYENEEFNLLQHALNVEDSIEGLRYNNV
                     VFATDADVDGMHIRLLLMTFFLQFFPDLVKNNHVYILETPLFRVRNKQETIYCYDEKE
                     KQAAMKKLGTKPEVTRFKGLGEISPDEFARFIGPDMRVQPVMIMPETHIQQLLEYYMG
                     KNTPQRQDFIIGNLRMEIDRTEQNGLVA"
BASE COUNT         1719 a         1787 c         1495 g         1809 t
ORIGIN      
        1 aaactacctg ggaacccatt ccgacaaaca ccttctttat tgcgactgga ccaaccggga
       61 cagccttttg gttacgcatc agcaatttga tcaggatatt gaccacgctt tcgaagaaat
      121 gaaaaattgg aatatcacta aacgaagggc ccgttatttt cttcctccct atgaatggta
      181 caacgaccgc attgcccgct ggagccggga agccggactg aaactggttt gttttacgcc
      241 cggaacccgc agcaatgcag actataccta tccggagatg ggcaacaagt atttatcggg
      301 cgccggtatt cttggatcca tccggttatt tgaaaaggaa tcgccggacg gattgaacgg
      361 ttttattcta ctcttgcaca tcggaaccga tccccgcaga aaagacaaga tgtatttcct
      421 gcttcccggg ctgatccgtg aattgaaaag caaaggatac cggtttgtta ccattccgca
      481 actgctcaga taaaatggcc ggtccgaacc ggaatgacgg cgccacggat ggcgtgaagc
      541 gcgatctgcc aagctccgca acccgtattc agactgaata ctatatttca tagttaaacg
      601 ttttataaat gattccggaa cgggaatact taacgttttt aacgcaaatt tcatttcgag
      661 acccacgaaa acaaccgtat ctttaagcca ttcagctgcc accaatctct attattcatt
      721 gccccgccca tgtataaagt ttcattcagt aataaagaca attcctttca ccaggaacta
      781 aaacgttccg tagatcagta tttttcaacc aaccatatca ataaaaccgg aaactggaag
      841 ctgtatatta aaacggctat cctggtccca tcggccattg ccatttattt gctcctgctg
      901 ctggttcatt tcccggtgtg gctggcgata gtgctttgca cgcttctcgg attcattttt
      961 gcgagcatcg gttttaatat catgcacgat gcctgtcatg gtgcctattc caaacgaacc
     1021 tgggtgaaca ctttaatggg cctcagcctg aacctgctgg gcggaaatgc ctttatctgg
     1081 aagttcaaac acaatattat tcatcacacc tataccaata tagacggact ggatgacgat
     1141 atcgcgaaga gtcccctcat gcggcagtgc ccttcccaga agtgggtgaa agcccaccgg
     1201 ttccagcatt tttatgtggt tctggtatat gctatatctt cttttgcctg ggtgactatg
     1261 atggatttca ataaatattt taaaaggaag atccataata ccagtttaca gaaaatggat
     1321 ctggaagaac atttcatttt ctgggcaagc aaggccatgt atgcgatatt ctatatcctg
     1381 atcccggttc attttgttgg ctggcaggcc tggggagtcg ggttcgcctg tatgcatatt
     1441 accatgggtc taaccctggc cctggtgttt cagctggccc atgtggtgga ggatgctgag
     1501 ttcgtatttg cacccggtat cgaaccgcaa aagctcgaag acgaatgggc gatccaccag
     1561 gtccgcacga cagccaattt tgctccgcgg aataaatttg tatcctggtt cgttggcggg
     1621 ctgaattttc agatcgaaca ccatcttttc ccgagagtaa gccacgtcca ttatcccgcc
     1681 atttcctcca ttgtaaaaca gatctgccgc aaccaccata ttcattacaa tgaattccgt
     1741 acgatgaccg gagccgtcgc ttcccatttc cgcatgatga aaatgctggg aagaaaaccg
     1801 gtgaactact gatcagaaaa taaaactccg cgtacgattc gatcatacac ggagcatcta
     1861 aaaaaccaca aggaacttac tgtgaagacg ggctcttata ataattcata atttcatcct
     1921 ggactgcctt gtccctgtct cttttgagtt cccggacttt cttccttctc tctttgtgcg
     1981 aaatgcgggt atcatgtctg acagaataga tccggtcggc ataatcattc ttaataccct
     2041 gaacattcat tgccagcttc gactgcctgt tatgccagcc gccattataa ggatatgcat
     2101 aaggtccgta aggactcata taaaagggag ggtaatatcc gtatagcccg aatggagaat
     2161 aatatcctaa cccgatactt acccggggcc ttacataata acccccgccg tgatagcttc
     2221 caccgtgatg aactgttcca ccccgatgtc cctgcgcggc ggctccaaaa acgagtccga
     2281 gtgacagcaa catcatcatt atctttttca taattcactg cgtttatatt attatatacg
     2341 caggaacctg aaaaaggatt gcggagtaac cgggtataac gattttctaa catttgctaa
     2401 caaatcctta agtatcagac agctggctat agggaaattc gctgatgacc gcccataagt
     2461 tttatttccc ttccattcca cacgaaacgt ccggttatat tttgcggaag cgtcacaacc
     2521 ccttctattc cgttcgttcc ctttcgtttc agggaaactt ccactgaacc ggcgggaatg
     2581 ggcatcaacc cttttatttc ttttaactcc cccaattgcg gactcaccag cactttttta
     2641 aaaccgggag cgtccggcat gataccgcaa agggtagcca gaaaatcata attcggactc
     2701 gcactccagg cgtggcagtc agagcgggtt ggatcgggct cttcggcaaa cgtggtcagg
     2761 cccattttta acatatttct ccagggcgtc aattgggaat aatacagatt tcccatgccc
     2821 gccctggtca ttgcgcgtgt aaggtaaaac cggtaataaa aagtggcctg gctaagcgtg
     2881 gaatcatata aaatatgatt cagcacctgc ttttgcaggt tttccggaat cgcatcagcc
     2941 agtatgccca taatgccggc atgctgactg aaactaacct tctccggcgt atttgccatc
     3001 tccattttct gcaggtcaaa acaatgctga taggtatttt ttgccagcaa atccgccagg
     3061 gccgaataat gcgcagcggc ttcattccgt ccataatatt taaataccgc agacgcctgt
     3121 cgcaaagtat acgcaaattg caggctgaca acagccgaat gcccgtctac ggctccatcc
     3181 ggcacaccat tcggaaatgc cttattccag tcagagaaat tccaccattt cataggcccc
     3241 agcattccca attttttatc caccctccgt tcaaaccatt tcaaaacaat atccgctccg
     3301 tcgagatatt tttccaaaaa ccggtcatcc tgacaatgca tccagtaatc gtacatcatg
     3361 gaaacccaga aaagcgaaaa aggagggatc acctgcaacc ggttgctggg atagcgccct
     3421 tgggtgagcc cttccggtac ccgggaatta aaaaaatcgt caatcgcttt ttgcatcagc
     3481 cggctgtctc ctgtattgta aagtgaaatc agggactgta tgcgggtgtc gccttcatac
     3541 tgcaactgct cataataggg acaatcgaaa taggtttccc cggcgcataa cctggcggta
     3601 cgccaacccg ttttccatat cgcttccagc gacccatcgt cagagctgaa agaggctttt
     3661 tgtacaaacg ggtaaccggt ataaattccg taaacatccg caatctcgag cggttcatct
     3721 tccgtctgca catcaatctg cagatagcgg taagtgcgga cccagagcgg actgaaactc
     3781 cggtcagcct gaccgtccgg ataaaaaatg tcgtacagac cttttatggt tttgtcctgg
     3841 atttcgttcc ggttgccctt ttgccccttt tgatcaaaca gggcttcggc ataggttaag
     3901 cggacaactg catttttacc ctggctcagc ctgatcaccg ggtaggctac tgtttcatag
     3961 gattgatcga gaaggatgct cagcttcgta tgcgccggaa tattcaatgg cctcgttccg
     4021 gaaataaatg ccggatcaat ggccagacca cttattctcc gtacaaccgc caaccgttgc
     4081 attttctcct ccatctgggg aatgctccgc ggagccagcg tccattggtt atccgttccg
     4141 tacccggatg ttacgacggg ggtaaacagt ggttttgcca tctgccagtt cccatcatta
     4201 taatcctttt gttcccagcc ccagggatag gctttccccc tgacctgatc gccaggcccc
     4261 accaccatat aggtatgcaa ttcggcgccc atattcccgg cacagggcgt ataagccgtg
     4321 tcatgcaaaa ctttccagga attgtctgaa ttcgccaggt tttccgctcc ggaatctccc
     4381 tgtaataaaa aacccgtttg attggatatc tgggcaacag gcgcccattc tcccatattc
     4441 cagaccaggg ccgcaatcac atttttacct gcctgcagat gagaagcaat atcaaaggaa
     4501 ccaaaattcc aattgtataa actgctccgg gcaggccctc ttccgatttc caccccgtta
     4561 acatatagca catagcggtt atccgcagaa acgtgaatga taaaacgggc gggcttgtcc
     4621 cgtagatcca tggtcttgcg gaaatgataa acgccataag cccggaaagg cacatccgga
     4681 caggtgatcc attgcgcatt ccatgacccc ttcagcagat ccggattgac cggcagttcc
     4741 tgagcgaatg cgaatgaaag ggaattgaag aaaaaaagaa caagggacag gcccatcagt
     4801 ttaccggaaa ataaggattt caaggtgaat gcttttatga agggattttc atttgaactt
     4861 actaaaagta aatgatttat ttcccaccgg tgtcctgcac tatcccggtt cggccactgt
     4921 ttccccgccc gccagccgca gcaattctcc ctgttgctcc agttcggcat tcgccaggag
     4981 ctgttttaaa cgccagatca aaaaagtttc ccccgtttta accggcgacc tggatagaaa
     5041 atgatttatt atccgggatg gtttctggaa ctccgccgtg acgaatttct tcagcggcgc
     5101 atcgtaataa tcatcttcct gctgaaccag ttttttggcg ccttccagtg tccggatatt
     5161 tttgttttct ccgccgaatt ttgtccattc atccggatcc gtttcaaatt cggacagcgt
     5221 cacgggccgg gccagttttt tagctttgat aaattcccgg ggtggaattt cagccaggcc
     5281 cgtaggataa aaaacgagcc ccttttcatt gataaatgga aggttattca gggaaaggag
     5341 atagatgcga ccggcaaatt cgttcaactg ggagatcagc caataatatc cgctgacgtc
     5401 ccgggcattc gaagccaccc agatccagat ctggtcaaac tcttcttccc gcatgcgttc
     5461 caggatctgc tgcaacttcg tatgatcggc cgggtcatat cccgcttctt cccgggaatt
     5521 tgccggattt aaaatcccgg cccaccaggt gcggcgcaat gcccttccgt cttcagaaaa
     5581 aagatccgtc accggaccca gggaataatc atctgatatc agcactatct cgccatccag
     5641 cgcctcatcc atatccatcg cggccgtaag cacctccgcg tcgcggggtt gaaaaacaat
     5701 atgaaacatg gaaaatattt ttatgccacc aatccgttct gttctgtgcg gtcgatctcc
     5761 atgcgcaggt ttccaatgat aaagtcctgg cgctgcggcg tgtttttgcc catgtaatat
     5821 tccagcagct gctgaatatg cgtttccggc atgatcatga cgggctgtac cctcatatcc
     5881 ggaccgatga agcgcgcaaa ttcgtccgga gatatttctc ccagtccttt gaaacgggtc
     5941 acttccggtt ttgttccaag ctttttcata gctgcctgct tctccttttc atcatagcaa
     6001 taaatagtct cctgtttatt ccgcacgcga aacaagggag tctccaggat gtacacatgg
     6061 ttgttcttaa cgaggtcggg gaaaaactgc agaaagaaag tcatgagcag gagccggata
     6121 tgcatcccgt ccacatcggc atcggtggca aaaacaacat tgttataccg caggccttca
     6181 atactgtcct ccacattcag ggcatgttgc aggagattga attcctcgtt ttcataaact
     6241 acctttttgg tcagcccgaa acaattcagg ggtttacccc ggagactgaa gacagcctgg
     6301 gtttccacat tacgcacctt ggtgatggac ccgctcgcag agtccccctc cgtaatgaag
     6361 atcgtgctct ccttttgctt ttcggcaatc agttctttgt ctttcccggt gggctcttca
     6421 ttataatgaa cccggcaatc ccggagtttg cgattgtgca gattggcctt tttggcccgt
     6481 tcattcgcca gcttgcggat accggataat tcttttcttt ctttttcgct ttgttcgatg
     6541 cgctttttca tcgcatcggc gatggacggg tttttatgca ggtaattatc cagttcacgg
     6601 gccaggaaat catgcacaaa attcttcatg ctcgcaccgc cctccactac atactggctg
     6661 cccagcttgg ttttggtctg ggattcaaaa acgggctcca ccacccggac agatacggcg
     6721 gccgtaagac tctgccggat atcagccgca tcataatcct tcttgtaaaa atcccggacg
     6781 acttttacaa aggcttcccg gaaagcctgc
//
LOCUS       DASYCU010000523         8909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4893639, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000523 DASYCU010000000
VERSION     DASYCU010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4893639"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2293)
                     /locus_tag="VGM24_10525"
     CDS             complement(<1..2293)
                     /locus_tag="VGM24_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155856.1"
                     /translation="MKKLSAKLLLLATSVLIFSFSRAADEQWPKQVTSTNGTLIKVYE
                     PAPESFEGNILKFRSAISITQNGSSEPVFGTFWASSKVETDRDNRQITFNTLNITDLK
                     IPSITDQNKLDEIDNALETQFPDAAGPLSLDNILTSLDQSRQEAKLSQGISTKAPKLM
                     FLTQPSMLVVIDGEPRLQRNPEWKLDVVVNTPFTIIKNDDGQFYLFGAKHWYVAAQAT
                     GPYRYTNGQVPANLSNIEQQIIDKEQSAAANNGNNNADQNAQAGDQSNTVPNIVVSTV
                     PAELIQSNGEPNFTPIQGTSLLYVNNSSNDIFMDENSQQYFVLISGRWYQAKTMTGDN
                     WKYIPADQLPADFARIPPGSQKDNVLASVAGTDAAKDAVMDAEIPQTAKVDRNTATTT
                     VNYDGRPKFTDIEGTDLQYAVNTSSTVIRAHNGRFYAVDNGVWFVSDNATGPWVVSTD
                     RPDELDQIPPNYPVYNTKYVYVYDVAPDYVYMGYTPGYLNSYIYGPTVVYGTGFYYDP
                     WFGNYYYPRPWSWGFNFGYNPWYGWSFGLGYNTGWFNYGFGYPGWGFGYGCSWWGPSF
                     YHPAYWGGWHGSRPYGYYGNSYGYRNNIRFNHSNNIYRNRNGVVTRSVGRGGSIRNPS
                     NRPESTRPGGRFNNPRPNSAGSRPGNGGNYNYRNNSAYGGRPSNVYSDRQGNVYQRNS
                     QGQWQQRQNRSWAPVNSRRPEVIQNLNRQQGMRERGEVRTQNFQRTMPQQSYRPSGGN
                     VSRPSGGSVSRPSGGGGSSRSSGG"
     gene            complement(2418..5237)
                     /gene="polA"
                     /locus_tag="VGM24_10530"
     CDS             complement(2418..5237)
                     /gene="polA"
                     /locus_tag="VGM24_10530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY4155857.1"
                     /translation="MEKKLFLLDAMALIFRAYYALIRSPRITSKGKNTNAQFGFTNTL
                     VELLNSQNPSHIAVCFDTEAPTERHIDFTDYKANRQEAPEDLIIAIPDIKKIIRGFQI
                     PIMELDGYEADDVIGTLSKQAADAGYEVFMVTPDKDYGQLVSDKVKIYKPGYQGGDVE
                     IIGPAEVLSKWNIKEVSQVIDLLGMMGDSVDNIPGIPGIGEKTAAKLLNEYHSLENVL
                     ANADNIKGAVGEKVKAGKELAIMSKKLACIITNVPVKFHEEDFKRKDWNKDALREIFA
                     ELEFRSVAKRILGEELPGPQGQKIQPEGKQQDLFGNAVAAKPLKEEEETEASPGLQPE
                     KNISNTPHQYMSADSPEKIQSLVSLLQKQNEVSFDTETTGIDANNADLVGLSFSWKAG
                     EGYYVPVPPDRGKALKILAAFQSLFDDPARNWVGQNIKYDLLVLKWYGVEIKGILFDT
                     MLAHYVIEPEGKRNMDLLSAQYLGYEPVHIEELIGKKGKTQGNMRDVELGKITEYAAE
                     DADITFQLKQKFFPLLKEKSVEDVFLKVDNPLVKVLTDMEFEGIRVDEDFLNEYSKTL
                     EKEAAQAEENVYRQAGVRFNLSSPKQLGEVLFDKLQLDPKAKKTKTGQYATGEDVLIK
                     LSHQSKIVDDILVYRELSKLKSTYVDALPVIINRKTGRVHTSYAQAVAVTGRLSSNNP
                     NLQNIPVRTERGREIRKAFIPRDKDHVLVSADYSQIELRIVAAISGDPNMVQAFKEGK
                     DIHSATAAKVFGVEETAVTKEMRYKAKSVNFGIIYGQGAFGLADNLGISRTEAREIIE
                     NYKKQFANIQKYMDDTINFARENGYVQTLMGRKRWLRDINSSNFTVRGFAERNAINSP
                     IQGTAADMIKLAMIKIHEAFKKNRFRSKMLLQVHDELVFDAHADEVEIIRPIILGAMR
                     QALPLPNEVPAEAELGVGANWLEAH"
     gene            complement(5317..7356)
                     /gene="uvrB"
                     /locus_tag="VGM24_10535"
     CDS             complement(5317..7356)
                     /gene="uvrB"
                     /locus_tag="VGM24_10535"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY4155858.1"
                     /translation="MEFKVHAPYQPAGDQPEAIRQLTEGILQGEKYQTLLGVTGSGKT
                     FTIANVIQNVQKPTLVLTHNKTLVAQLYGEFKQFFPDNAVGYFVSYYDYYQPEAYMPV
                     SDTYIEKDLSINEELDKLRLQATTQLLSGRRDIIVVASVSCIYGIGNPDDFENGIIRI
                     KRGQTISRQGFLHGLVNSLYTRDQFEFKRGSFRVQGDTVDINLPYLDYGYRVTFFGDQ
                     IDEIESFEMKTGKRIQPMDDAAVFPANLYLAPKHLIQQILQEIQDEMAAQVNYFKSSG
                     KFIEAQRLSERVNYDVEMIRELGYCNGVENYSRFFDRRRPGTRPFCLLDYFPDDFLCM
                     IDESHQTIPQVSGMYGGDRSRKLILVDYGFRLPSALDNRPLNFHEFESMTGQTIYVSA
                     TPGDYELEKTEGVVIEQVVRPTGLLDPPIEIRPSVNQIDDLLDEIDKRITKGDRVLVT
                     TLTKRMAEEMDKYLARINIKSKYIHSEVDTLERVEILRQLRLGEIDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNERSLTQTAGRAARNVDGLVIFYADKMTESMQRTIDET
                     SRRRMKQIEYNEAHGITPKTILKSRQQVFAQTSVLDIKGYDEKNPYAIAGDQDLVTTA
                     AEEEAEYSTIPQMQKGISRIKKEMEKAARDLDFMEAARLRDQMFSMQKKLTEMKQ"
     gene            complement(7532..>8909)
                     /locus_tag="VGM24_10540"
     CDS             complement(7532..>8909)
                     /locus_tag="VGM24_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEY4155859.1"
                     /translation="ENSLLRFTEVNGTWQDMKFHSDSMVIRNLIQPTMSCNIKSRFKL
                     EALNNFFEDGTFSFSNGSGRADLQFRGPLTEKSSVSNSLNGVFLLDSATFTYLPRNFE
                     LSNGSGKIRFSGPDMFIEDLHVNTGSSDLLMNGSMKNLFSLMDKNSSPLALDWSIRSN
                     RLNLDDFKSFLKKRSGAVSKKKKKLLFSETISNMTRLLETSSMRLNLQAKQLSYKKFT
                     ATGLHSSMELNDDAINIKTVHLEHAGGVIEAQGSLRNEINSNPFSLNATLIKVDLSKV
                     LDAFNNFGQHALTSKNIQGKLSANIDLKGEVTQKAQVIPDSTRGQIDFNLQEGRLIQF
                     EPVQKISQTVFKNRNFSDIQFADLHDLFNINGNTLTINRMEIRSTVMTMFVEGEYNIK
                     KGADLSIQVPLSNLKNKSDLIVPENQGINSKTGLSARLRAKNGDDGKLRITWDPFNKA
                     VRKMKKKK"
BASE COUNT         1932 a         2261 c         2181 g         2535 t
ORIGIN      
        1 caccgccgga acttctggat gaaccaccgc cgccggaagg cctgctcact gatccgccgg
       61 aaggtctgct cacgttacct ccggagggac gataggattg ctgtggcatt gttctctgga
      121 aattctgtgt ccgcacttct ccgcgttcgc gcattccctg ctggcggttc aaattctgaa
      181 ttacttccgg ccgcctggaa ttcacagggg cccagctccg gttttgcctt tgctgccact
      241 gaccctggga attacgctgg taaacatttc cctggcggtc agaatacacg ttggacggcc
      301 ggccgccata ggctgaatta ttccggtaat tgtagtttcc gccatttccc ggtctggaac
      361 cggccgaatt cgggcggggg ttgttgaacc ttccaccggg acgggttgat tccggacggt
      421 tacttgggtt tctgatactg cctccgcgac caaccgaacg ggtaactacc ccgttccggt
      481 tcctgtaaat attgttggaa tgattgaacc ggatattatt tctatagccg taggaatttc
      541 cgtaataacc gtagggcctt gaaccgtgcc atccacccca gtaagccgga tgataaaagg
      601 acggacccca ccagctgcag ccataaccga atccccagcc tgggtaacca aacccataat
      661 taaaccagcc ggtattgtac cctaatccga agctccagcc ataccaggga ttatatccaa
      721 aattgaatcc ccaggaccag ggtctcgggt aataataatt cccgaaccac ggatcataat
      781 aaaaaccggt tccgtatact acggtcggac cgtagatata actgttcaga tacccgggtg
      841 tataacccat gtatacatag tccggagcca catcatagac ataaacatat ttggtattgt
      901 ataccgggta attcggtggg atctggtcaa gttcatccgg ccggtctgta ctcaccaccc
      961 agggaccggt cgcattgtcg gatacaaacc aaactccgtt atcgaccgca taaaaacggc
     1021 cattgtgggc gcgaatgacg gtggaagagg tgtttacagc gtattgcagg tcggttcctt
     1081 caatatccgt aaacttgggt cgaccgtcat aatttaccgt ggtggttgcg gtattccggt
     1141 ccaccttggc tgtttgcgga atctcggcat ccatcaccgc atcctttgcc gcgtctgtgc
     1201 cggccacgct ggccagcacg ttgtccttct gcgatcccgg agggatcctg gcaaagtcag
     1261 ccggcaactg atcggccgga atatacttcc agttatcccc ggtcatcgtt ttcgcctgat
     1321 accatcttcc ggagatcagc acaaagtatt gctgggaatt ttcatccatg aaaatatcgt
     1381 tgctggaatt gtttacatat agtaaagaag ttccctggat gggcgtaaaa ttcggttcgc
     1441 cgttcgactg gatcagttca gccggaacgg tgctgacaac aatattggga accgtattcg
     1501 actgatcgcc tgcctgcgcg ttctgatcag cattgttatt gccattattt gccgccgcac
     1561 tttgttcttt gtcaatgatc tgctgttcta tattgctgag atttgccggt acctggccat
     1621 tggtataacg ataaggaccg gtggcctgcg cggcgacata ccaatgtttc gcaccaaaga
     1681 gataaaattg gccgtcgtcg tttttgatga tggtaaacgg cgtgtttacc acaacgtcca
     1741 gcttccattc gggattgcgt tgcagccggg gttctccgtc tataaccacc agcatggaag
     1801 gctgggttaa gaacatcagc ttgggcgctt tggtggagat accctgggaa agcttggctt
     1861 cctgcctgga ctggtccagg gaagtgagga tattgtccag ggataagggg cctgcagcat
     1921 ccgggaactg ggtttccagt gcgttatcaa tttcatcgag tttgttctga tccgtgatcg
     1981 aagggatctt gagatcggtg atattgaggg tattaaaggt gatctggcgg ttatcccggt
     2041 ccgtttccac cttggaactg gcccagaaag taccgaatac cggctccgaa ctcccgtttt
     2101 gtgtaatgga aatggccgaa cggaatttca ggatgtttcc ctcaaaagat tccggggccg
     2161 gttcatatac cttaatcagt gttccatttg tactcgtaac ctgtttaggc cactgttcgt
     2221 ctgccgccct gctgaagcta aaaatcagca ccgaggtggc taacagtaag agtttcgcag
     2281 agagcttttt cattgtgtag tggtttttat tcaattgcat caattttttg atcccggtag
     2341 ggatgcgtgc ttaatttaag actgctcagc gggaatatag tttgaagttc aattcagaat
     2401 aaaatgttaa agtgtaacta atgggcttcc agccagttag cgccgactcc cagctcggct
     2461 tccgccggga cctcattggg cagtggcagc gcctggcgca tcgctccgag aatgatgggc
     2521 cggatgattt ccacctcgtc cgcatgggcg tcgaatacca gctcatcgtg cacctggagc
     2581 agcattttgg atctgaacct gtttttttta aaggcttcat gaattttgat catggccagt
     2641 ttgatcatat ccgcagccgt tccctggatc ggggaattaa tggcgttccg ttccgcaaat
     2701 cccctaacgg tgaaattcga tgaattgata tcccgcagcc agcgtttacg tcccatcagg
     2761 gtttgaacgt atccgttttc ccgcgcgaaa ttgatggtat catccatgta tttctgaata
     2821 ttcgcaaact gcttcttgta attctcaatg atctccctgg cttctgttct ggatattccc
     2881 aggttgtcgg ccagtccgaa ggcgccctga ccgtaaatga tcccgaaatt gacacttttt
     2941 gccttgtagc gcatttcttt ggtcacggct gtttcctcca ccccgaatac tttggccgcc
     3001 gtggcgctgt gaatatcttt tccctccttg aatgcctgca ccatattggg gtctccgctg
     3061 atggcggcaa cgatccggag ctcaatctgg gaataatcgg cggagaccag tacatggtct
     3121 ttgtcccggg gaataaacgc ttttctgatc tcccggcctc tttcggtccg cacggggata
     3181 ttctgcaggt tcgggttatt gctggacagc cgcccggtga ccgccaccgc ctgcgcataa
     3241 ctggtgtgca cccgcccggt ctttcggttg atgataaccg gtagtgcgtc cacataagta
     3301 gattttaatt tgctgagttc acgatacacg aggatatcat ccacaatttt actctggtgg
     3361 ctcagtttga tcagaacgtc ttcccctgtg gcatattgac ccgttttggt ttttttcgcc
     3421 ttcgggtcca gctggagttt atcaaataac acctcgccca gttgtttggg ggaagaaagg
     3481 ttgaaacgca cgccagcctg ccggtagacg ttttcttccg cctgcgccgc ttctttttcg
     3541 agcgttttgg aatattcatt taaaaagtcc tcatccaccc ggatgccttc aaactccata
     3601 tcggtgagca ctttcacgag cgggttatcc actttcagga atacatcctc caccgatttt
     3661 tccttcagga gcgggaaaaa tttctgtttg agctgaaagg tgatatcggc atcctctgct
     3721 gcatattcgg tgatttttcc gagttccaca tcgcgcatat ttccctgggt cttccccttt
     3781 tttccgatca gctcttcgat gtgaaccggt tcataaccga ggtattgggc gctgagcagg
     3841 tccatattcc ttttgccctc cggttcaatt acataatgtg ccagcatggt gtcgaacaga
     3901 atgcccttta tttccacgcc ataccatttg agaaccagca gatcgtattt gatattttgt
     3961 cccacccagt tgcgtgccgg gtcatcgaac agggattgaa aggcagcgag gatctttaat
     4021 gccttcccgc ggtccggcgg aacaggaaca taataacctt ctcccgcctt ccaggaaaaa
     4081 ctcagaccca cgagatccgc gttgttggcg tcgatcccgg tggtttccgt gtcaaaggaa
     4141 acttcgtttt gcttttgcag cagggaaaca agggattgga ttttctccgg gctgtctgcg
     4201 ctcatatact gatgcggcgt attggaaata tttttttccg gttgaaggcc cggggaagct
     4261 tcggtctcct cctcttcttt taatggcttt gccgcgacgg catttccgaa cagatcctgc
     4321 tgtttgcctt cgggttgaat tttttgtccc tgcggtccag gcagttcttc ccccagtatc
     4381 cttttggcca cgcttctgaa ttccagttcg gcgaatattt cacggagggc gtccttattc
     4441 cagtccttcc gtttaaaatc ttcttcatgg aatttcacgg gaacatttgt gatgatgcac
     4501 gcgagtttct tactcatgat ggccagctct ttaccggctt ttaccttttc tcccacagcc
     4561 cccttgatat tatctgcatt ggccagtaca ttttcgagtg aatggtattc gttgagcagt
     4621 ttggcggccg ttttttcacc gatgcccggg atacccggaa tattgtccac cgagtcgccc
     4681 atcataccca gcaggtcgat gacctgggat acttctttaa tattccattt actcagcact
     4741 tcagccggac cgataatctc cacatcgcct ccctggtatc cgggcttata aatttttacc
     4801 ttatcgctga cgagctgccc gtagtcttta tcgggagtaa ccataaaaac ctcatagccc
     4861 gcatccgctg cctgtttgct gagggtgccg atgacatcgt ccgcttcata accatccagt
     4921 tccatgatgg ggatctggaa tccccggatg atctttttga tatccggaat ggcaatgatc
     4981 aggtcttcgg gcgcttcctg ccggttggct ttataatctg taaaatcaat atgacgttcg
     5041 gttggcgcct ccgtatcaaa acaaacggca atatgggagg gattctgcga attgagtaac
     5101 tccaccagcg tattggtgaa tccgaactgc gcattggtgt tttttccttt ggacgtgatg
     5161 cggggactgc ggatcagcgc atagtaagcg cggaagataa gcgccatcgc atcgagtaaa
     5221 aataattttt tctccatgag ggttagttcg tttttaattt ttaatctgcc cgtttatttt
     5281 ccgggtgaaa cccgccagcc ggcaaattct ttccaactac tgtttcatct ccgtcagttt
     5341 cttttgcatc gaaaacatct ggtcccgcag gcgggccgcc tccatgaaat ccaggtcccg
     5401 cgcggctttt tccatttctt tttttattct ggaaatgcct ttctgcattt gcgggatggt
     5461 gctgtattcc gcttcttctt ctgccgcggt ggtcaccagg tcctgatctc ccgcaatcgc
     5521 atacggattt ttttcatcat atcccttgat atcgagcacg gaagtctggg caaatacctg
     5581 ttgacgggat ttcaggatcg ttttcggagt aatgccatgg gcttcattat attcaatctg
     5641 tttcatcctc cggcggctgg tctcgtcaat ggtccgctgc atgctttctg tcattttatc
     5701 ggcgtaaaag atcacgagcc cgtccacgtt tcgcgcagcc cgtcccgcgg tttgggtcag
     5761 ggatctttca ttcctcaaaa atccttcttt gtcggcatcc aggatggcca ccagggaaac
     5821 ttccggaagg tccagtcctt cccggagcag gttcacccca accagaacat caatctcgcc
     5881 aagccgcagc tggcgcagga tttcaaccct ttccagggta tccacctcgc tgtggatata
     5941 tttcgatttg atattgatgc gggccaggta tttatccatt tcttccgcca tgcgcttggt
     6001 gagcgtggtc accagcacgc ggtccccttt ggtaatccgc ttgtcaattt catccagcag
     6061 gtcgtcgatc tgatttacgc tcggacggat ttctatcggc ggatcgagca atccggtggg
     6121 acggacaacc tgttctatca ccacaccttc cgttttctcc agttcatagt cgcccggcgt
     6181 ggcggataca tatatggttt gtccggtcat ggattcaaat tcgtgaaaat tgagcgggcg
     6241 gttatccagc gcggaaggca ggcggaagcc atagtctacg aggatgagct tcctgctccg
     6301 gtcgccgccg tacatgccgc tcacctgggg aatggtctga tggctttcat cgatcatgca
     6361 tagaaaatca tccgggaaat aatcgagcag gcagaacggc cttgtccccg gtcttctccg
     6421 gtcaaaaaac cgggagtaat tttccacccc gttacaatag ccaagttccc ggatcatttc
     6481 cacgtcgtaa ttcacgcgtt cactgagcct ttgtgcttct atgaatttgc cgctactctt
     6541 gaaatagttt acctgggcag ccatttcatc ctggatctcc tgcaggatct gctggatcag
     6601 gtgttttggc gcgagataaa ggtttgccgg aaaaacggcc gcatcgtcca tcggctggat
     6661 acgcttaccc gttttcattt caaagctttc aatttcatca atctgatcgc cgaaaaaagt
     6721 tacccggtat ccataatcca ggtagggcag attgatatcc acggtatctc cctgcacgcg
     6781 aaaagaacct cttttgaatt cgaactgatc ccgggtgtaa agggagttga ccaggccatg
     6841 caaaaatccc tggcgggaaa tggtctggcc ccgttttatg cggataatgc cgttctcgaa
     6901 atcatcgggg ttcccgatac cgtagatgca gcttaccgaa gcgaccacaa tgatatcgcg
     6961 acggccgctc aggagttggg tcgtcgcctg caacctgagc ttgtccagct cttcattgat
     7021 cgaaagatcc ttttcaatat aggtatcgct aaccggcata taggcttccg gctgataata
     7081 atcatagtag gaaacaaaat agcccacggc attatccggg aagaactgtt tgaattcacc
     7141 ataaagctgg gccaccaggg ttttattatg ggtgagcacc agcgtcggtt tctgcacgtt
     7201 ctggatcaca ttggcgatgg tgaatgtttt tcccgagccg gttacaccaa gcagggtttg
     7261 atatttttcg ccctgcagaa tgccttccgt aagttgccgg atcgcctccg gctgatcgcc
     7321 cgccggttga tagggagcat ggactttaaa ttccattcag catatttttt tttggagcct
     7381 gtaaagttac cgattaatat ttattgagca tgcatttgcg gcagaggaaa ccgcgttacc
     7441 gcgaatacaa ggagcgggct gaatggcctg catgttttct accggtttat ttaaaaaaag
     7501 gtagaaacac ccgggatcat ccggaagaag atcatttctt ttttttcatt ttgcggactg
     7561 ccttattaaa agggtcccag gtgattctga gttttccgtc atccccgttt tttgcacgga
     7621 gcctggcact cagcccggtc ttgctgttta taccctggtt ttccgggaca atgagatctg
     7681 atttgttctt cagattactg agcggcacct ggatgctcag gtcggctccc ttctttatat
     7741 tgtattctcc ctccacaaac atggtcatta ccgtggagcg gatctccatc cggttgatgg
     7801 tgagggtatt cccgtttatg ttaaaaaggt cgtggagatc ggcaaactgg atatcggaga
     7861 aattccggtt cttaaaaacg gtttgggaaa ttttctgcac gggttcaaac tggatcagtc
     7921 ttccctcctg cagattaaaa tcaatctgcc ccctggtgct gtcggggatc acctgtgctt
     7981 tttgagttac ctcgcctttc aggtcgatat tggcgctcag cttgccctgg atatttttgc
     8041 tggtaagggc gtgctgacca aaattattga atgcatccag caccttgctc aggtccacct
     8101 taatgagggt ggcgttaagg gagaaaggat ttgaattgat ttcatttcgc agggagccct
     8161 gggcttcaat gacgcctccg gcatgttcca ggtggaccgt ctttatattg atggcatcgt
     8221 cgttcagttc catactgctg tgaagtccgg tggcagtgaa ttttttatag ctcagttgtt
     8281 ttgcctgcag gttaagccgc atgctgctgg tttccagcaa ccgggtcata ttggaaatcg
     8341 tttctgagaa cagaagtttc tttttctttt tggaaaccgc tccggatctt tttttaagga
     8401 aagatttgaa gtcgtcaagg ttcagccggt tactccggat actccagtcc agagcgagcg
     8461 gtgagctatt tttatccatc agtgaaaaaa ggttcttcat gctgccgttc atcagcaggt
     8521 cactgcttcc ggtattcacg tgcaggtcct cgataaacat atccggaccg gaaaacctga
     8581 ttttaccact tccgttcgaa agttcaaaat ttcggggcag gtaggtaaag gttgcggaat
     8641 ccagtagaaa gacgccgttc agcgagttgc tgacagaact tttttcggta agcgggcccc
     8701 tgaattgaag atctgcccta ccgctgccgt tgctgaagct aaatgttccg tcttcgaaga
     8761 agttattgag cgcctccagt ttaaaacggg atttaatatt acagctcata gtgggttgaa
     8821 taaggttccg gatgaccatg gaatcggaat gaaatttcat atcctgccag gtcccattca
     8881 cctctgtaaa acgcagcagg gagttctcg
//
LOCUS       DASYCU010000524         3949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1421946, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000524 DASYCU010000000
VERSION     DASYCU010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421946"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VGM24_10545"
     CDS             complement(<1..922)
                     /locus_tag="VGM24_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY4155860.1"
                     /translation="MEKRKLGKSGLEVSALGFGCMGLSFGYGPAMDKKQAIKLLDAAV
                     ERGVTFFDTAEAYGPFENEELLGEALAPYRDQVVIATKFGFKEGRPSSGTDSRPERIR
                     AVAEAALKRLRTDRIDLFYQHRVDPNIPMEEVAGTVKELIGEGKVKYFGLSEAGVDAI
                     RRAHTVQPVAALQSEYSLWWREPEAEILPALEELGIGFVPFSPLGKGFLTGAINANTS
                     FDKTDFRNTVPRFSEENRKANQALVDELTQIAADKGATPAQIALAWLLAQKPWIVPIP
                     GTTKLHRLEENLGAAGIRLNTGDLKTIREAL"
     gene            1075..1182
                     /locus_tag="VGM24_10550"
                     /pseudo
     CDS             1075..1182
                     /locus_tag="VGM24_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            1247..2587
                     /locus_tag="VGM24_10555"
     CDS             1247..2587
                     /locus_tag="VGM24_10555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY4155861.1"
                     /translation="MKCSAIIKGLLPVFIIAVLSGCTGEGKMLGNTEISKWQYGKKGA
                     VSLTYDDGSSNQFSQALPIMRRLKLPATFFVITGPIAGSRYQAKFVGRPVKEIIRETA
                     KVPTNADNFFERASAARYLGYKGTIAYYDRSDAKYERGKKDEAYAIMDSLYAKVRSGT
                     LEPGETLSNEVMQEKGLTWDSLKQYAAEGYEPASHTVTHAHLAELDTANMMYELEKSK
                     ADIEEHLGKEYTFSAEVPYGIEDPRVMKYGLPVYLALRNSMPEPFMKEINRGYKDEPG
                     ASDKEYVQWQRGPLSKTPLSKMESWIDTTRKHDNIWLVLVFHGVDDIGWEGIRHELLD
                     TYFQYIKKQEDSLWIATFGEVGRYIRERMNGKTEDQVSDSGITVNLKTSLDTSIYHVP
                     LTLKTYVPAAWKKVQVAQGDKIKSALPENDTKGTYILYQAAPDAGPVKLSEDKT"
     gene            complement(2599..3396)
                     /locus_tag="VGM24_10560"
     CDS             complement(2599..3396)
                     /locus_tag="VGM24_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEY4155862.1"
                     /translation="MDHWITLIRSKLETWLTGFIKLLPNLTLAILIFVVFFLFSKYVR
                     RVVNRFLLKVSGKPAISGLFSTIIYIIFLFAGLFISLELLHLEKTISSLLAGAGIIGL
                     ALGFAFQDLTANFISGVFIIFRKPFEVGQIVDSNGFIGTVEEIKLRSTTIRTYQGLHI
                     MLPNKDIFQKPITNYSLSGDRRIDISLVLPVKTNTGQLEQTLKKALSDIPGIKKEKPP
                     EIYFTDYTGDTIKIEIWCWIDNKTPMGFTDTKDKIIKKVHDALNPAG"
     gene            complement(3496..3924)
                     /locus_tag="VGM24_10565"
     CDS             complement(3496..3924)
                     /locus_tag="VGM24_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155863.1"
                     /translation="MKKSFLKALVLPCAAVAAFNLGQDNTTNLLEVRPDPWPINLQRI
                     ITRSDTSYALEFRDQQVLTGVMLDTLPFGNLEQLRYFEQGLSALKKGTNGDIANYRNY
                     SIKRTNVYKQPTWYILRFRSGLTNFQQPEADKMIAAIRGL"
BASE COUNT         1005 a          983 c         1005 g          956 t
ORIGIN      
        1 ccaatgcttc cctgattgtt ttcagatcgc cggtgtttaa gcgaataccg gctgcgccaa
       61 gattttcctc caaccggtga agtttggtgg ttcccggaat aggcacgatc catggctttt
      121 gggcgagcag ccaggccagg gcgatctgtg ccggggttgc ccctttatcg gctgcaattt
      181 gcgtcagctc gtcaaccaaa gcctggtttg ctttcctgtt ctcttccgaa aagcggggaa
      241 ccgtattccg gaagtccgtt ttgtcgaaag aagtgtttgc gttaatagcg ccggtaagga
      301 accctttacc cagcggactg aaaggaacaa acccgattcc aagttcttcg agtgccggca
      361 ggatttccgc ttcgggttcg cgccaccaca acgaatattc gctttgcaat gcggcgaccg
      421 gctgcaccgt atgcgctctc cggatcgcat ccactccggc ctctgaaagt ccgaagtatt
      481 ttaccttgcc ttcaccgatc agttctttta ccgttccggc cacctcttcc atcggtatat
      541 tcgggtctac ccggtgctgg taaaacaggt ctattcggtc ggtacgaagg cgctttagcg
      601 cggcctctgc caccgcacgg atgcgttccg gcctgctatc cgtgccggat gatggtctgc
      661 cctctttgaa gccgaatttt gtggcgatca ctacctggtc ccggtagggc gccagggctt
      721 cgcctaacag ttcctcgttc tcgaacgggc catacgcttc cgccgtatca aaaaaggtca
      781 cgcctctctc cactgcggcg tccaataatt tgatggcttg ctttttatcc attgccgggc
      841 catagccgaa actcagcccc atgcaaccaa agcccagcgc cgacacttcc aggccgcttt
      901 ttcctaattt tcttttttcc atttttttag attgatttta tggcaacgcc gttatttaat
      961 tttacttttt ccatttgccg ttttattcaa cggcacaaag atcctatccc ccaccttaat
     1021 aaacaggata cggatcacgg aagcttgtac catttttact gacacggcaa aacggtcagc
     1081 caggtcgcct acggacttgg cttcaaatac ccgcaacatt ttgccaggct cttcaaacag
     1141 aaagtgggac agtcgccgct ggaataccgc tcgcttaatt agcgtaacaa caattggtta
     1201 tctttaagga accggatgct tattattaaa tcctaaaata ccagcgatga aatgctcagc
     1261 cataataaaa ggattactgc ccgtatttat cattgcagtc ctgagcggtt gtaccggcga
     1321 aggaaaaatg ctcggaaata cggagatcag taaatggcaa tacgggaaaa aaggagcggt
     1381 ttcccttacc tacgatgacg gctcctcgaa ccagttcagc caggccctgc cgatcatgag
     1441 gcggttgaaa ttaccggcta ctttttttgt cattaccggc ccgatagcag gatcccgtta
     1501 ccaggcaaag tttgtcggac gtccggtaaa ggagatcatc cgggaaacgg cgaaggttcc
     1561 caccaatgcg gataattttt ttgaacgggc ttctgcagcc cggtatctcg ggtataaagg
     1621 cacgattgcg tactacgacc ggtcggatgc aaagtacgaa cgcggaaaaa aagacgaggc
     1681 ctatgcaatt atggattccc tgtacgccaa agtgagaagc ggcaccctgg aacccggtga
     1741 aacactcagc aatgaagtga tgcaggaaaa aggactcacc tgggacagtc tgaaacaata
     1801 tgccgccgag ggatatgaac ccgcaagcca tacggtcacg catgcccatc tggccgaact
     1861 ggacaccgcc aatatgatgt acgagctgga aaaaagcaag gcggatatcg aagaacactt
     1921 gggaaaagaa tatacgttct cggcggaagt tccctatgga attgaagatc cgagagtgat
     1981 gaaatacgga ctcccggtct acctggcgct cagaaacagc atgccggaac cctttatgaa
     2041 agaaataaac cgcggataca aggacgaacc gggcgcttcg gataaggagt atgtacaatg
     2101 gcagcgaggc ccgctttcca aaacgccatt atcgaagatg gaatcctgga ttgataccac
     2161 ccgcaagcat gataatatct ggctcgtgct ggtctttcac ggggtggacg atattggctg
     2221 ggaagggata cggcatgaat tgctggacac ctattttcaa tacataaaaa aacaggaaga
     2281 cagcctctgg atagccactt tcggggaagt gggccggtat atccgggaac ggatgaatgg
     2341 gaaaacagaa gaccaggtca gcgacagcgg aattacagtg aacctgaaga ccagcctgga
     2401 tacatcgata tatcatgttc cgctgaccct aaagacctat gtgcctgcgg cctggaagaa
     2461 ggtgcaggtg gcacagggag ataaaattaa aagcgccctt ccggagaatg ataccaaggg
     2521 aacgtatatc ttatatcagg cggctccgga tgccgggccg gttaaactct cggaagataa
     2581 gacctagcat ttcatgcctc agcccgcagg gttgagcgca tcgtgcactt tcttaatgat
     2641 cttgtctttt gtatcggtga aacccatcgg cgttttattg tctatccagc accagatctc
     2701 gatctttatc gtgtcgccgg tataatccgt gaaatatatt tcagggggct tttccttttt
     2761 gattccgggg atatcggaaa gggctttttt cagggtctgt tccagctgtc cggtgttcgt
     2821 tttaacgggc aataccaggc tgatatcgat ccggcggtct ccgctcaaag aataattcgt
     2881 gatgggcttt tgaaaaatat ccttgttcgg aagcataatg tgtaatccct ggtaagtccg
     2941 gatggtagtc gaacgcagtt tgatctcctc caccgtaccg ataaacccgt tgctgtccac
     3001 aatctggccc acctcgaagg gtttacggaa aataataaat acgcccgaaa taaaattggc
     3061 ggtaaggtcc tggaaggcaa atcccaacgc caggccaatg atgccggcgc cggccagcag
     3121 cgaagagatg gtcttttcca ggtgcagcaa ctcgaggctg atgaacaggc cggcaaaaag
     3181 gaaaataata taaataatgg tcgagaaaag tccgctgatg gccggcttgc ccgaaacttt
     3241 cagcaggaag cggtttacca cacgccgtac gtatttggaa aaaaggaaga aaacgacaaa
     3301 gatcagaata gccagcgtca ggtttggcag cagtttaatg aagccggtaa gccaggtctc
     3361 caatttcgat ctgatcagtg taatccagtg gtccataaat ctacggcccg gtaatttttg
     3421 aatatttcgg aaattccgga atcttcataa ccgtttaaaa aaaccatgcc atcggtttta
     3481 aggatctccg caggattaca atcctctgat ggcggcgatc atcttgtcgg cctcgggttg
     3541 ctggaaattg gtgagcccgc tgcggaaccg caggatatac caggtgggct gtttatatac
     3601 gttggttctt tttatggaat agttcctgta attggcaatg tcgccattgg tccctttctt
     3661 caacgccgac aaaccctgct cgaaatatct cagctgttcc aggttcccga agggaagggt
     3721 atccagcatc acgccggtga gcacctgctg atcccggaat tccagggcat aggaagtatc
     3781 tgatctggtt atgatccgtt gcaggttgat gggccaggga tccggccgca cttccaggag
     3841 gttagtggta ttgtcctgac ccaggttaaa cgccgcgacg gctgcacagg ggaggaccag
     3901 ggccttcagg aaggattttt tcatagcatc tgggatttac cggccaaaa
//
LOCUS       DASYCU010000525         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4753148, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000525 DASYCU010000000
VERSION     DASYCU010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4753148"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VGM24_10570"
     CDS             <1..1068
                     /locus_tag="VGM24_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155864.1"
                     /translation="KKESRDFLLYVPVPAQTGFTQAYRNVGSLSNSGVEFAVNYSHST
                     SPAFHYNIGVNLTTVNNKLLSLASGEDFIYNLASLGFPTTGSNNWGTYSKSSVGGPVG
                     EFFGYKSAGIFQNQKEIDDLNSVAQAKYGAGNYYQPTSSGSAVIGDRKFIDVNGDSKI
                     TADDQVPLGSPIPKFYGGVNFDATYKNFDFSLFFYGTYGNKIFNYQERTLESFESSTG
                     SVGIENIGLKYYQNAWSSSNPSNRYAIISANDFNVNTRPSDVYVEDGSYLRLRNLTIG
                     YTLPVKLSSANFAPKIRIYFTGQNLFTITKYTGLDPEIGIPQGTDPNTGASVRNVTAS
                     GIDVGTYPSSRYFTFGLNVTF"
     gene            1100..2731
                     /locus_tag="VGM24_10575"
     CDS             1100..2731
                     /locus_tag="VGM24_10575"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155865.1"
                     /translation="MKNYKNIYLVALVLLFLPAACSKNFLNQKDTSHSSEESLFHTPQ
                     DGISLVNAIYDGFQHDNQSFVLKALWYNANYCSQDYFNWGADVSYNTYLFPVDFGSTS
                     VYWNLSYQGIARANSAIPIIDKMHTEGVISDSLANFLTGQAYFLRGVFYYYLACTFGG
                     VPLELKTVTDNGLHPRSTQDSVFMSVASDMTTAAALLPWPEDLPQSDLGRATKGAALG
                     YLGAAQMWLKQYSDAVATFNQVIPHYQLMNKYMDIHEYDHQNNAESIFELQFALPGGA
                     TPDWSYNNNEVTWNSSFMWPWETSNFGYTYCNQKLYTSFEAGDTRKPATIVGPGDTLA
                     SPGIVAIGGIKAYAQVIAGFAGTLSLPKAHFTGTDGKIINTCGKVSDPWTGDQPGQPR
                     SGYYGTKFWRDPNVSGNTPSAVDGKTNIFGDQNVTMLRLGEIYLSKAEAQFQSGDQPG
                     AEATLAIVRNRAWGNTTAPLSPFGPDFMQIMLQEYRHELSGEMSLWYDLRRTGLQIAY
                     IKNTFGIDIPAGHDLLPIPITAIASNPTLKQNPNY"
     gene            2836..>3603
                     /locus_tag="VGM24_10580"
     CDS             2836..>3603
                     /locus_tag="VGM24_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY4155866.1"
                     /translation="MKKTHHLFLLLPLWLFCACHGKKTLFEKISSSHSGIDFNNKIVE
                     NDTINPLDVVNIYNGGGVGIGDFNRDGLPDIYFAGNQVPGRLYLNQGNFKFLDITQKA
                     GVAGLGRWARGVSVVDINNDGLMDIYICNTIYKDSLRRRNILYVNQGPDKDGIPHFKD
                     MAAEYGLDIHGQSTMASFFDYDNDGDLDMYLTVNEASNGDNPSVFLQRDRGKLTPSMG
                     RLYRNDMDSSLRHPVFHDVSASAGIKYEGYGHAATICD"
BASE COUNT          946 a          886 c          861 g          910 t
ORIGIN      
        1 aaaaaggaat cgagggattt tctgctgtat gtacctgtgc cggcccaaac cggtttcacg
       61 caggcctacc ggaatgtggg cagcctgagc aacagcgggg ttgaatttgc agttaactat
      121 tcgcattcca cttccccggc cttccattat aatattggcg ttaatctcac caccgttaac
      181 aacaagctgc tgagcctggc gagtggtgaa gactttatct ataacctcgc cagtctggga
      241 tttcccacca ccggctccaa taactgggga acctacagca agtcgagcgt aggcggcccc
      301 gttggcgagt ttttcggata taaatccgcc ggcatttttc agaaccagaa agagatcgat
      361 gatctgaatt ccgtggcaca ggctaaatac ggagcgggta actattatca gcctacgtcg
      421 agcggaagcg ccgttatcgg ggaccggaaa tttatcgatg taaacggcga cagcaaaatc
      481 acggcagatg accaggttcc tctgggaagt ccgatcccga aattttacgg gggagtgaat
      541 tttgatgcta cctataagaa ctttgacttc agcctcttct tctatggcac ctacgggaat
      601 aagatcttta actaccagga aagaacgctg gaaagctttg aaagttccac cggatcggtc
      661 ggtattgaaa acatcggcct gaaatactat cagaatgcat ggtcgtcctc gaacccatcc
      721 aaccgctatg ccataatatc tgcgaatgat tttaacgtga atacccggcc ctctgatgtg
      781 tacgtggaag acggcagtta cctgcgtctc cgaaacctga ccatcggtta taccttgccg
      841 gtaaaattgt cgagcgccaa ttttgcgccc aagatcagga tctattttac aggacaaaat
      901 cttttcacga taaccaaata caccggtctc gatccggaaa tcggtatacc gcagggtacg
      961 gatccgaata ccggcgcttc tgttcgtaat gtaacggctt ccggaataga tgtgggaacc
     1021 tatccttcat ccagatattt tactttcgga ttgaacgtga cattttaatt tcaatatgaa
     1081 aagtcttaaa aacggagata tgaaaaatta taaaaatatt tacctggtcg ccctggtgct
     1141 tcttttcctg cctgcggcct gttcaaagaa tttcctgaat caaaaggaca cctcgcattc
     1201 cagtgaagag tctttgttcc acaccccaca ggacgggatc agcctggtaa acgccattta
     1261 tgacggattt cagcatgata atcagagctt cgtgctgaag gccctttggt acaatgccaa
     1321 ttattgttcc caggactatt tcaactgggg ggcagacgtt tcttacaata cgtatctgtt
     1381 cccggtggat ttcggatcca ctagcgttta ctggaacctt tcttaccagg gtatcgccag
     1441 agccaattct gccataccga tcattgataa aatgcacacg gaaggagtga tcagcgattc
     1501 cctggccaat tttctcaccg gtcaggccta tttcctgcgg ggcgtgtttt actattatct
     1561 ggcctgcacg ttcggaggcg ttccgctgga gttaaagacc gtgaccgata atggtcttca
     1621 tccccggagc acccaggatt ccgtatttat gtctgtcgct tccgacatga ccactgccgc
     1681 cgccttattg ccctggccgg aggacctgcc gcaatcggac ctgggaaggg ccaccaaggg
     1741 agctgccctg ggttatctgg gcgccgcgca gatgtggctg aaacaatatt ctgacgcggt
     1801 ggccaccttt aatcaggtta tcccccacta ccagctcatg aataagtata tggatatcca
     1861 tgaatatgat catcagaata atgcggaatc gatctttgaa ctccagttcg cccttccggg
     1921 cggagcgact ccggattgga gctataacaa taacgaagta acctggaact cctcatttat
     1981 gtggccctgg gaaacgtcta attttggtta tacttattgt aaccagaaat tatacacttc
     2041 ttttgaggcg ggagacaccc gcaagcccgc aaccattgta ggcccaggtg atacgctggc
     2101 cagcccgggt atcgtggcga tcgggggaat aaaagcatat gcacaggtca ttgcggggtt
     2161 tgcgggcact ctttcgcttc ccaaagcaca tttcaccgga acagacggca agatcatcaa
     2221 tacctgcggt aaggtttctg atccgtggac tggcgaccag cccggtcagc caaggtccgg
     2281 atattacggc actaaattct ggcgcgaccc caacgtaagc ggtaatacac ccagcgcggt
     2341 cgacggaaaa acaaatatct tcggcgacca gaatgtgacc atgctccggc tgggcgaaat
     2401 ttacctcagt aaggcagaag cacaattcca aagcggtgat caaccgggcg ctgaagctac
     2461 cctggctatc gtaagaaacc gtgcctgggg aaatacgact gcacctcttt ccccttttgg
     2521 gccagatttc atgcagatca tgctgcagga ataccggcat gaactcagcg gggaaatgtc
     2581 tttatggtat gatctccgga gaacaggtct gcagatcgcc tatataaaaa ataccttcgg
     2641 gatagacatt cccgcaggtc atgatttgtt acccattccg ataaccgcca tcgccagcaa
     2701 cccgacatta aagcaaaatc cgaattatta agattagcag gaaattaaaa agaaggctct
     2761 gtatttttac cgggccttct tttatttcgg gtgattaccc gggatcctga actgtttttc
     2821 agctattccg tttttatgaa aaaaactcac catctgtttc ttctgttgcc gctgtggctt
     2881 ttctgtgcct gtcatggtaa aaagacgctg tttgaaaaaa tttcttcttc ccactcgggt
     2941 attgatttca ataataaaat agtagagaat gacaccataa acccgctgga tgtggtgaat
     3001 atatacaatg gcggaggcgt cggcatcggt gatttcaaca gggacggcct gcccgatatc
     3061 tattttgccg gcaaccaggt tcccggccgg ttatatctca accaggggaa ttttaaattc
     3121 ctggacatta cccaaaaagc aggagtcgct ggtttaggaa gatgggccag aggggtttcc
     3181 gttgtcgata taaacaacga cgggctgatg gatatttata tctgtaacac aatttataag
     3241 gattcgctaa gaaggcgcaa tatattatac gtcaaccagg gcccggacaa agatggtatt
     3301 ccgcatttta aagatatggc tgccgaatac ggactggata ttcatggaca atccacgatg
     3361 gccagtttct ttgattacga caacgatggc gacctggaca tgtaccttac cgtcaatgaa
     3421 gcatccaatg gcgataaccc gtctgttttt cttcagcggg accgcggcaa attaacgcca
     3481 agtatgggaa gattgtaccg gaacgatatg gacagcagcc tgcgacatcc cgtattccat
     3541 gatgtttcgg catcggccgg catcaaatac gaaggatacg gccatgccgc cacgatttgc
     3601 gat
//
LOCUS       DASYCU010000526         8580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_113562, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000526 DASYCU010000000
VERSION     DASYCU010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113562"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VGM24_10585"
     CDS             <1..601
                     /locus_tag="VGM24_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155867.1"
                     /translation="LDTVFDENHMEKMKFAENFIKISIERLFRVNGSSAQETGVIPDI
                     LLRDFTDTRSERERTMPFALANTQIEPNKYYHPYPALPLDSLKAFARAFSDTCTYLRS
                     FNHYLDQLLEMQVPKNEPLSLEKVRQGQERMNSFLAEREKSANAVSLPYEIQWNAFEK
                     IRMRTDEDLRNTNQQLTNVLLKDPGLLLGYQLASRMLQK"
     gene            614..1675
                     /locus_tag="VGM24_10590"
     CDS             614..1675
                     /locus_tag="VGM24_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155868.1"
                     /translation="MPKPTRMRRYFYFILILSLFLHTEARSQIIENAGDYMTQIAGAR
                     VEMDQKYMAYISAAAHSRRARKVEKLRAQTLESIDDSKSKTMGLPKYKGDNSLRQSSI
                     DYIQFCYRIFNDDYSKIVNMEEIADQSVDQMQAYLLLEEKTAEKLEEASAKLSRAEKD
                     FAGKYSVTLVESKNELYDKMVSAGEINRYCDKLYILFFKCNWEDGQLFNALNSHKIND
                     AEQARNALIHFAEEGLKALNSDSLRDFKGNSGLKNACRQALEFYDRMAGNEIPKLTDF
                     YLKQENFDKIKKSMEAKGSNRTRQDVEEYNKAVKEMNAGAGNFNQLTQDLNKRRQEVS
                     LNWENSEKSFRDDLMPYYK"
     gene            complement(1644..2672)
                     /locus_tag="VGM24_10595"
     CDS             complement(1644..2672)
                     /locus_tag="VGM24_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEY4155869.1"
                     /translation="MKAVVIIAFLASGALFWVNTPFPFPIPKGFPPPVYHFDKNPLSK
                     EGFELGRKLFYDGKLSIDGNFPCSSCHQQFAAFATYEHRLSHGFNNQFSYRNAPALFN
                     LAWQTAFHWDGGINHLEVQPLAPLLDPHEMAESLDHVINKLKQDKQYPALFSKAFGSP
                     GINSQRILRALAQFTVSLVSANSKYDRVKRGEAVFSQYEKPGYEIFKQKCAGCHREPL
                     FTDLSYRNTGLAADSVLKDYGRMRITGRREDSLKFKVPSLRNVFLTFPYEHDGRFNTL
                     SDVLEHYNSGVIQSATLDSFLRKGIPLTENDKFYLIQFLGTLTDSSFINNPLFKDPVT
                     YNKASGHP"
     gene            complement(2669..3496)
                     /locus_tag="VGM24_10600"
     CDS             complement(2669..3496)
                     /locus_tag="VGM24_10600"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family protein"
                     /protein_id="HEY4155870.1"
                     /translation="MRQHPIILIFTRFWSNRFGLVPGIILAMGISAFQTGRKPLPSGI
                     GISDPPNLILHFKVTVHGQPFQLHKTYLNPFGESFTPDIFRFYAGKITASYKNGAQVK
                     GHSPESYHLIDFADSASTQVALRIREGVYNEIHFLLGIDSIDQVGGAQSGPLDPVKGM
                     FWTWNSGYLSFKIEGSSPKSTEPAHAFAYHIGGYRWPNRTSRNIKIGTGNGRPFRINR
                     EGATVLNIPLELDHFFDGITPIHIRNIPACTSPGRLARQIAENFTAVFTGIEPAALP"
     gene            complement(3493..3897)
                     /gene="arfB"
                     /locus_tag="VGM24_10605"
     CDS             complement(3493..3897)
                     /gene="arfB"
                     /locus_tag="VGM24_10605"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY4155871.1"
                     /translation="MKIDVSREITFRTARSGGKGGQNVNKVETMVLGYFHIGNSSLLS
                     NEQKAVLHQKLSSKINADGFLLVKSQEHRSQLENKEEVVKKIGHLIERALIKPKPRKA
                     TKPSFASKEKKKESKQKKSQTKNLRKRISRFE"
     gene            3938..4390
                     /gene="dtd"
                     /locus_tag="VGM24_10610"
     CDS             3938..4390
                     /gene="dtd"
                     /locus_tag="VGM24_10610"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY4155872.1"
                     /translation="MRAVIQRVSRASITINGSETAAIGRGLLILVGIEAGDGPEDIEW
                     LSRKIIQLRIFDDDAGVMNRSLMEIGGELMLVSQFTLFASTRKGNRPSYIRASKPEAA
                     IPLYESLIAKIEKDFGRKIGTGIFGADMKMELLNDGPVTILMDTKNKE"
     gene            4387..5175
                     /locus_tag="VGM24_10615"
     CDS             4387..5175
                     /locus_tag="VGM24_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY4155873.1"
                     /translation="MMELKIYIANAFAGDRFGGNPAAVVPLQDWLSDELMQQIAAQNN
                     LAETAFIVPQGNDFAIRWFTPTVEVALCGHATLASAHIFFRHLGYGREQIRFHSGSGP
                     LLVSKRPDGKLTLDFPADEPVPADSSAFLEQGLKVKPIELYKSSFDYLAILDGQDKIE
                     RLSPDFGLLAKLPSRGVIVSAKGDQSDFVSRCFFPQSGIDEDPVTGSAHTVMVPYWAA
                     RLGKSALTAVQLSKRRGYLDCALQGDRVLMSGQAFTYLAGEIYI"
     gene            5197..5541
                     /locus_tag="VGM24_10620"
     CDS             5197..5541
                     /locus_tag="VGM24_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEY4155874.1"
                     /translation="MEEISIRQAQQLIDQWIRTTGVRYFSELTNMAVLTEEVGEVARL
                     MSRLYGDQSFKENELQKTLPDELADVLWVLICIANQTGVDLTAALQKNMEKKNIRDAE
                     RHQANRKLKGES"
     gene            complement(5542..6993)
                     /locus_tag="VGM24_10625"
     CDS             complement(5542..6993)
                     /locus_tag="VGM24_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY4155875.1"
                     /translation="MKIAVAKGEGIGPEIMDAVLSIFNANQVPIEYEVVEMGKWVFDK
                     GFSNGMTPEAQHAIEELGILFKGPMETPKGKGVKSVNVTARKTWNTYANKRVFQSLHG
                     VETVFSRAGIPIDITIVRENIEDTYGGIEHMPTHDVALSRRFITRPGSLQVIKYAFEM
                     AKKNGARRITCGHKANIMKLTDGLFLECFQEVAKQYPELKSDDIIVDDLAMKLVVRPQ
                     DFDVVVLTNLQGDIISDLCAGLVGGLGFAPSANIGDHIAIFEAVHGTAPDIAGKNIAN
                     PTGLLLSGIAMLRHLGFMQNAAVIENALLYTLEQGVHTGDFGDKAIPGKNTTEFSQAI
                     IGNFGKTPDHGAKAVVPNMHVTPTVFKLDHNQMMVSEETLKEYILGVDMFIESSEQPE
                     YIAKKCQRHGGVKFNLVNISNRGTQVWPTGSVYTNLVNLYNVRFESLDGSPLNQQDVL
                     GLYVSLSGDFKICSSELLNMLGGKKAYSLAQGQ"
     gene            7115..7501
                     /gene="apaG"
                     /locus_tag="VGM24_10630"
     CDS             7115..7501
                     /gene="apaG"
                     /locus_tag="VGM24_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY4155876.1"
                     /translation="MANKISEGVEIDVETFYQPDYSNPVAGEHMFAYRITIWNHNNFP
                     VKLHRRQWHIFDSNGTHREVEGEGVVGVQPVLQPGEKYQYVSGCNLRTEMGKMYGSYN
                     MENLNTKTVFEVNIPVFEMIVPFKMN"
     gene            complement(7542..>8580)
                     /locus_tag="VGM24_10635"
     CDS             complement(7542..>8580)
                     /locus_tag="VGM24_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155877.1"
                     /translation="VQKEDMPLAKIVSYVFLPAIVAGIIGWFIPGNLMGSDSNDAFIF
                     ACIPIIIFYFNIWYRCNPSDRRRVGTLFTLFGVSIIFWNIYSQNATSLTIWADSYTKR
                     EAPAAIEKVIKPFGFLQTVNTDPKEVPVLDEHFHAQVGAKGKVMTHLGTDPYLQNIPK
                     DQWPAKGEDLKLISTEIYQSVNPFWIIVLTPVMVGLFGFLKASGRELSTPGKFAWGTI
                     IAGLSSVIMVIAALSTNIYEHKVSSAWIVSSYGIFTISELCVSPVGLSLVSKVSPRRF
                     TALMMGGWFITTSLGAKIAGIMAGFWDNFDSKALFFGISAIAAIVAGLLLIPLTKKLN
                     KVVVEATARED"
BASE COUNT         2200 a         2022 c         2188 g         2170 t
ORIGIN      
        1 gctggatacc gtgtttgatg aaaatcatat ggaaaaaatg aagtttgccg aaaattttat
       61 taagatatct attgaaaggc ttttccgggt gaacggttcg agtgcccagg agacgggtgt
      121 gattccggat atcctcctgc gggattttac ggatacccgt tcagagcggg aacggacaat
      181 gcctttcgcg ctggccaata cccagattga acccaataag tattatcatc cctatccggc
      241 gcttcccctg gacagcctga aggcctttgc ccgggcgttt tcagatacct gcacttacct
      301 ccggtccttc aatcattacc tggatcagtt actggaaatg caggttccga agaacgaacc
      361 gctttccctg gagaaagtga ggcaggggca ggagcgtatg aattcctttt tggcagagcg
      421 cgagaaatcc gcgaatgcag tatcccttcc ttatgaaatc cagtggaatg cctttgaaaa
      481 aataagaatg cgcacggatg aggacctccg gaatacgaat cagcagctta caaacgtttt
      541 attgaaagat ccgggcctgc tgctgggcta tcagcttgct tcccggatgt tacaaaaata
      601 attctattca gccatgccaa aacctacccg aatgcgccgg tatttttact ttatcctgat
      661 cctcagttta tttctccata cagaagccag gtcccagatt attgaaaatg caggcgacta
      721 tatgacccag atcgccggcg cgagggtgga aatggatcag aaatatatgg cttatatcag
      781 cgcggcggca catagccgcc gggcacgcaa ggtggaaaaa ctgagagcgc aaaccctgga
      841 aagcattgac gactccaaat cgaaaaccat gggtcttccc aaatacaagg gggacaattc
      901 gctgcgccag tccagtattg actatatcca gttctgttac cggattttca atgacgacta
      961 ttccaagatc gtgaacatgg aagaaattgc cgatcaatcg gtggaccaga tgcaggctta
     1021 tcttttgctg gaagaaaaaa ctgctgaaaa actggaagag gcttctgcca aactctccag
     1081 ggccgaaaaa gattttgccg gtaaatacag tgtaaccctg gtggaatcga aaaatgagct
     1141 ttatgataaa atggtttcgg caggggaaat aaaccggtac tgcgacaaac tctatatcct
     1201 cttctttaaa tgcaactggg aagacgggca gctgttcaat gcactgaaca gtcataagat
     1261 caatgatgct gaacaggccc ggaatgccct gattcatttt gcagaagaag ggttaaaagc
     1321 attgaattca gacagcctcc gggatttcaa aggaaattcc ggattgaaaa atgcctgccg
     1381 ccaggccctt gagttttatg accggatggc aggaaacgaa ataccgaaac tcaccgattt
     1441 ctatttgaaa caggagaatt ttgacaagat caagaaaagc atggaagcca aaggaagcaa
     1501 ccggaccagg caggatgtgg aagaatacaa taaagcagta aaagaaatga atgccggcgc
     1561 cggtaacttt aaccagctga cgcaggacct gaataaacga cgccaggagg tgagcctgaa
     1621 ctgggaaaat tccgaaaaat cctttaggga tgacctgatg ccttattata agtaaccgga
     1681 tccttaaaca ggggattatt aatgaatgag ctgtcggtga gcgttcccaa aaactgaatg
     1741 agataaaatt tatcattttc agttaacgga atgcccttcc gcaggaagga atcgagggtg
     1801 gcactttgaa taacaccgga attataatgt tccagcacat cgctgagggt attaaagcgg
     1861 ccatcgtgtt cgtacggaaa ggtcaggaat acattgcgca gactgggtac tttaaacttc
     1921 aatgagtcct ctcttctacc ggttatccgc atgcgcccgt aatcttttaa cacactgtct
     1981 gccgcaagcc cggtgttgcg atagctgaga tcggtaaaca ggggttcccg gtggcagccg
     2041 gcgcattttt gcttaaagat ttcatacccg ggtttttcat actgggaaaa aacagcttcg
     2101 cctcttttca cccggtcata tttggaattg gcagagacca gggataccgt gaactgggcc
     2161 agcgcgcgta atatccgctg cgaattaatt ccgggacttc caaaggcttt gctgaatagc
     2221 gcgggatatt gtttatcctg tttcagttta ttaatgacat gatcgaggct ttccgccatt
     2281 tcgtgcggat ccagcagggg ggccagcggt tgtacctcga ggtgattgat gcccccgtcc
     2341 caatgaaaag cagtctgcca ggccagattg aataaggcgg gcgcattccg gtagctgaac
     2401 tggttattga atccatgact cagccggtgt tcatacgtgg caaatgccgc aaattgctga
     2461 tggcaggatg aacagggaaa gtttccgtca atcgaaagtt ttccgtcgta gaatagtttt
     2521 cgcccgagtt caaatccttc ttttgaaagc ggatttttat caaagtggta gactggcggg
     2581 gggaatcctt tcggaatggg gaaagggaaa ggagtattca cccaaaaaag cgcgcccgat
     2641 gccagaaaag ctatgatcac aacggctttc atggcaaggc agccggttca atgccggtga
     2701 ataccgcggt gaaattttca gcgatctgcc gggccagcct gccgggtgag gtacaggccg
     2761 gtatgtttcg gatatggatg ggcgttatac cgtcgaaaaa atggtcgagt tcaaggggaa
     2821 tattcagaac ggtggctcct tcccggttta tccggaatgg ccgtccattg cctgttccga
     2881 tttttatgtt ccgggaagtg cggtttggcc aacggtaacc tccgatatgg taggcaaagg
     2941 catgggcggg ttctgttgat tttggcgagc tcccttcgat tttaaaactg aggtagccgc
     3001 tgttccaggt ccagaacatt cccttaaccg gatcgagcgg gccgctctgc gcgcccccga
     3061 cctggtcaat cgaatctatt cccagcaaaa aatgaatttc attgtaaacc ccttcgcgga
     3121 tccggagggc gacctgcgtg gaagcggagt ccgcaaaatc aatcaggtgg tatgattctg
     3181 gagaatgccc ttttacctgc gccccatttt tataggaggc ggtgattttc ccggcataaa
     3241 accggaagat atccggcgta aaggattcgc cgaaagggtt taaataggtt ttgtgcagct
     3301 ggaacggctg tccatgcacc gtaactttga agtgaaggat cagattgggc ggatcggata
     3361 taccgatgcc ggacggaaga ggtttcctgc ccgtttggaa tgcgcttatt cccatggcca
     3421 ggatgatccc gggcaccagc ccgaaacggt tgctccaaaa gcgggtgaat attaatatga
     3481 tgggatgttg cctcattcaa agcgactgat cctctttctt agatttttgg tttgcgactt
     3541 cttctgtttg gactccttct ttttctcttt cgacgcaaaa ctgggtttgg ttgccttacg
     3601 gggtttgggt ttgatcaggg cccgttcgat caggtgtccg atttttttta ccacctcttc
     3661 tttattctcc agctgtgacc ggtgctcctg gctttttacc agcagaaaac catctgcatt
     3721 tattttggaa gaaagttttt gatgcagcac cgccttctgt tcattgctga gcagactgga
     3781 atttccgata tgaaaataac caagcaccat cgtctccact ttgttgacgt tttgtccgcc
     3841 cttgccgccg ctgcgggctg tcctgaaagt gatttctctg gaaacgtcta tcttcatttg
     3901 acgaaagtaa atggaaaatg tgtaaatgta ttgccccatg cgtgcggtaa tccaaagagt
     3961 ttcccgtgcc tccattacca ttaatgggtc tgaaacagct gctatcggcc gggggctatt
     4021 gattttagtt gggattgaag ccggggatgg cccggaggat attgagtggc tgagcagaaa
     4081 aatcattcag cttcggatct ttgatgatga cgccggtgtg atgaaccggt ccctgatgga
     4141 gatcggcggg gaactgatgc tggtaagcca gttcaccctg tttgcttcaa cccggaaggg
     4201 gaatcgtcct tcctatatcc gggcctcgaa gccggaagcg gccattcccc tctatgagtc
     4261 cctgatagcg aaaatcgaaa aggatttcgg aagaaagatc gggacgggta tttttggtgc
     4321 ggacatgaaa atggagctgc tgaatgatgg accggttact attttaatgg atacgaaaaa
     4381 taaggaatga tggagcttaa aatttatata gccaatgcct tcgccggcga ccggttcggc
     4441 gggaatcccg cagccgttgt tcccttacag gactggctga gtgatgaatt gatgcaacag
     4501 attgcggccc agaacaatct cgccgagacg gcttttatcg ttccccaagg caatgatttc
     4561 gccatccgct ggttcacacc aacggtggaa gtggctcttt gcgggcatgc caccctggcg
     4621 tctgcgcata tttttttcag acacctgggt tacggcagag agcagatccg gtttcattcc
     4681 gggagcggac cgctcctcgt atcaaaaaga cctgatggga aattaacgct tgattttcca
     4741 gctgatgaac cggttcctgc tgacagttca gcattccttg aacagggatt gaaagtaaaa
     4801 cccattgagc tttataagtc ttctttcgat tacctggcca tcctggacgg gcaggataaa
     4861 attgaacgtc tttcacccga tttcggtctg ttggccaaac tgccttcccg gggagttatc
     4921 gtcagcgcaa aaggggatca atccgatttt gtttcccgtt gcttttttcc gcagtccggt
     4981 attgatgaag atcccgttac cggatccgca cataccgtga tggttcctta ctgggcggcc
     5041 agattaggga aatcagcgtt aacggctgtt caattgtcta aaagaagggg ttacctggat
     5101 tgtgcattgc agggagaccg ggtgctcatg agcgggcagg catttactta tcttgcggga
     5161 gagatttata tttaagcact aaaaaagccg atgtttatgg aggagatttc catccggcag
     5221 gcccagcagc tgatcgatca atggattcgc actaccggcg tgcgctattt cagcgaacta
     5281 accaatatgg ccgtcctgac cgaagaggtg ggtgaagtgg cccggctgat gagccggctc
     5341 tatggcgatc agtcctttaa ggaaaacgaa ttgcaaaaaa cccttcccga cgagttggcg
     5401 gacgtgctgt gggtattgat ttgcattgcc aaccaaaccg gtgttgattt aaccgctgcc
     5461 ctgcaaaaaa acatggaaaa gaaaaacatc cgggacgcag aaaggcacca ggctaaccgg
     5521 aagctgaaag gggaaagctg attattgtcc ctgcgccagg ctatacgcct tcttccctcc
     5581 gagcatattt aacagttcag acgaacagat cttgaaatcc ccgctgaggc tgacatagag
     5641 gccgagaaca tcctgctggt tcaatggaga gccgtccagg ctttcaaaac gcacgttata
     5701 caggtttacc agattcgtat atacggaacc ggtaggccat acctgggtcc cgcggttgct
     5761 gatgttcacg agattgaact ttacgccgcc atggcgctgg cattttttgg caatgtactc
     5821 cggctgttca gaactttcaa taaacatatc cacccccaga atatattctt tcagggtttc
     5881 ttcagaaacc atcatctggt tgtgatccag cttgaatacg gttggtgtaa catgcatatt
     5941 cggcaccacc gctttggcgc cgtggtccgg cgttttgcca aaatttccga taatggcctg
     6001 ggaaaattcg gtggtatttt tgccgggaat ggccttgtct ccgaaatcac cggtatgcac
     6061 gccttgttca agcgtataca acaaggcgtt ttcaataacg gcggcattct gcataaaacc
     6121 cagatgacga agcatggcga taccgctcag taagagaccg gtggggtttg caatattttt
     6181 tccggcgata tccggcgctg ttccatgaac ggcttcaaag atcgcaatat ggtctccgat
     6241 atttgccgaa ggcgcaaatc cgagtcctcc caccaaaccc gcacagagat cggaaatgat
     6301 atcgccctgc aggttggtca gcaccaccac gtcgaaatcc tgcggccgca ccaccagttt
     6361 catggccagg tcgtctacaa taatatcatc tgatttcaac tccggatatt gttttgccac
     6421 ttcctggaaa cattccagga aaaggccatc cgttagcttc atgatattgg ctttatgccc
     6481 gcaggtgatg cgccgggcgc cgttcttctt ggccatttca aaggcatatt tgatcacctg
     6541 caggctgccc gggcgggtaa tgaaccggcg gctaagggcc acatcatgcg tgggcatatg
     6601 ttcaatgcca ccataagtat cttcaatatt ttcccggacg atggtaatgt ctatgggaat
     6661 accggccctg gaaaaaactg tttccactcc atgcagggat tgaaaaaccc gtttgttggc
     6721 ataggtattc caggttttcc gggcggtaac attcacgctt ttcactcctt ttcccttcgg
     6781 tgtttccatg ggccctttga agagaatgcc cagttcttcg atggcatgct gggcctcggg
     6841 cgtcatcccg tttgaaaagc ctttgtcgaa tacccatttt cccatctcga ccacttcgta
     6901 ttcaattggc acctgattgg cgttgaaaat ggaaaggacg gcgtccatga tctccggacc
     6961 gataccctct ccttttgcta ctgcaatttt catgataaaa gattggtaag tctgctgttg
     7021 agcgcgaatg tacgataaaa gggttgaccg ataaccggtg gggtttgact tcaacttttc
     7081 atttcatttc cttaatttta tacaaattaa ctccatggcg aacaaaattt cagaaggggt
     7141 tgaaatagat gttgagactt tttaccagcc ggactacagc aatccggtcg ccggggaaca
     7201 tatgtttgcc taccggatca ctatctggaa tcacaataat tttcccgtga aactgcaccg
     7261 tcgccaatgg catatttttg acagcaacgg aacgcaccgg gaagtggagg gagaaggcgt
     7321 ggtgggtgtg cagcctgttc tgcaaccggg agaaaaatat caatatgtga gcggttgcaa
     7381 tctgcgcacg gaaatgggga aaatgtacgg cagctacaat atggaaaacc tgaataccaa
     7441 aaccgtattc gaagtgaata ttccggtttt tgaaatgatt gttcccttca aaatgaactg
     7501 aacgcttccg gagggaattc cccgccgccg gatttccccg atcagtcctc cctcgcggtc
     7561 gcttccacca ccaccttatt caattttttt gtaagcggaa tgagcagcag gccggcgaca
     7621 atcgcagcga tggcgctgat gccgaagaat agagccttgc tgtcgaagtt atcccagaag
     7681 ccggccatga tgccggcaat ttttgctccg agtgaagtgg tgatgaacca gccacccatc
     7741 atcagcgcgg taaaccgcct cggggatacc ttggagacca gggataatcc cacgggactt
     7801 acgcataatt cgctgatcgt gaaaatgccg taactgctga cgatccaggc cgaggaaacc
     7861 ttatgttcgt aaatattggt agacagcgca gcgatcacca tgatcacgga agaaagcccg
     7921 gcaatgatgg ttccccaggc aaatttccca ggggtgctga gttccctgcc gcttgccttc
     7981 aggaagccga ataatccaac catgacgggc gtgagcacga tgatccagaa cgggttaacg
     8041 gattgataaa tttccgtgga aatgagtttg agatcttctc cttttgccgg ccattgatcc
     8101 ttgggaatgt tctgcaggta aggatccgtt cccaggtggg tcatcacttt tccttttgct
     8161 cccacctgtg catggaagtg ttcatccagc accggtactt ctttcggatc cgtattcacc
     8221 gtctggagaa acccgaaagg cttaattact ttttcaatcg cggccggcgc ttcgcgtttc
     8281 gtatagctgt cggcccagat cgtgagggaa gtggcattct ggctgtagat attccagaaa
     8341 ataatggaaa cgccaaaaag ggtgaacagc gtaccgaccc gtcttctgtc ggaaggattg
     8401 cagcggtacc agatattgaa ataaaaaata ataatgggaa tgcaggcaaa tataaacgcg
     8461 tcattggaat cggaacccat caggttcccg ggaataaacc acccgatgat accggccaca
     8521 atagccggaa ggaagacata agaaacaatc tttgccaggg gcatatcttc tttctgaacc
//
LOCUS       DASYCU010000527         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1563873, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000527 DASYCU010000000
VERSION     DASYCU010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563873"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VGM24_10640"
     CDS             complement(<1..219)
                     /locus_tag="VGM24_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155878.1"
                     /translation="MYAEIIIPLALPKNYTWSVPENLHLQPGCRVEVSLRNKKYAGII
                     KRFVEEKPEAFDPKPIGQVLDTEPLIYPE"
     gene            complement(267..1040)
                     /locus_tag="VGM24_10645"
     CDS             complement(267..1040)
                     /locus_tag="VGM24_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY4155879.1"
                     /translation="MKVGTSFIRIIYSIYALLWFVLFTLIAFPLVIVFSFFGRIAGGN
                     AICNLSKVWSDAWYFLLGIRHQNIFESGYDPGRTYVFVSNHISYLDIPCIFKAIRKQT
                     FRVLAKADLKKVPVFGYIYSRGAVMVDRSSTSNRAQSVRELKKLLAHHVSVFIFPEGT
                     FNETGDPLKSFYDGAFRISIQTQTPIKPMLFLDNYSLMNYHSIFSLKPGRSRVIFLEE
                     ISPEGYTLNGIHLFKEKVYGIMDRKLRDYQASWINSQPR"
     gene            1145..2470
                     /locus_tag="VGM24_10650"
     CDS             1145..2470
                     /locus_tag="VGM24_10650"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEY4155880.1"
                     /translation="MAEAKTVAFHTLGCKLNFSETSSLSRLMQQAGFEKKEFGTDADV
                     YVINTCSVTENADRECRYLVRQIQRKSPESFVVITGCYAQLKPQAIAAIPGVDLVLGA
                     AEKFNLAEHISQLVKGDAAKICSCDIEDVQSFHEGVSFNDRTRAFLKVQDGCDYTCSF
                     CTIPLARGKSRSDTIPHVLDTVRELAEKGIREVVLTGVNLGDFGFSAPAGKTSGTPVY
                     EKEKTNDLLKKKPENFFELTQALDRMTEMPRFRISSIEPNLLTNDIIGLVADSQRFMP
                     HFHIPLQSGSNKILGLMRRRYRRELYSDRVQLIKTRMPHAAIGADVIVGFPGETEADF
                     KETVDFLHSVDVSYLHVFTYSERDNTRALDIKPEVPVSVRNERNKILRNLSFQKQKYF
                     TELHAGQTRKVLFERSRSQGMMEGYTDNYIKVTTPYRAEWANRIVDWRL"
BASE COUNT          674 a          648 c          676 g          611 t
ORIGIN      
        1 ttccgggtag atcaggggct cggtgtccag cacctggccg atgggtttcg gatcgaaagc
       61 ctccggtttt tcttccacaa aacgcttgat gatcccggcg tattttttat ttcgcagcga
      121 cacttccacc cggcatcccg gttgaaggtg gaggttctcc ggaacagacc aggtatagtt
      181 cttcgggagc gccaggggta tgatgatttc tgcgtacaaa ttttctcctc ttgtttagat
      241 tttaaacaat gaaacgggct acaagttcag cgcggctggg aattgatcca ggaggcctgg
      301 tagtccctga gtttgcggtc cattatgccg tacacttttt ctttgaaaag atgtattccg
      361 ttcaaggtat acccttccgg actgatttcc tccaggaaaa tcaccctgga tcttccgggt
      421 ttcaggctga aaattgaatg ataattcatg agtgaataat tatccaggaa gagcatcggt
      481 ttaatggggg tctgtgtttg tatcgaaatc cggaaagcgc cgtcgtaaaa agatttcagc
      541 ggatcgccgg tctcgttgaa cgttccttcc gggaaaataa ataccgaaac atgatgggcc
      601 aggagttttt tcagctcgcg gacgctctgg gcgcggttgc tggtgctgct ccggtccacc
      661 atcaccgccc cgcggctgta gatatatccg aataccggta cttttttcag gtccgctttg
      721 gccagcacgc gaaaggtctg tttccggatg gccttaaaaa tacaggggat atccaggtag
      781 gaaatatggt tgctgacaaa aacataggtc cggcccggat catagcccga ttcaaaaata
      841 ttctgatgcc ggatgcccag cagaaaatac caggcatccg accagacctt ggaaagatta
      901 catatcgcgt tgcctccggc aatccttccg aaaaaagaga aaacgatcac cagcgggaaa
      961 gcaatcagcg taaaaagtac gaaccagagg agtgcgtaga tgctgtaaat gatccggata
     1021 aaactcgtcc caaccttcat tgaattcctt tctgtgaaaa cgaaaataac taataagggg
     1081 caaacccggg aggcgcaaag atccggctcc attatttccg ggcgggctgt atctttgcgg
     1141 acttatggcc gaggcaaaaa ccgtcgcttt tcataccctg ggatgcaagc tgaatttttc
     1201 agaaacgtct tccctgtcgc ggctgatgca gcaggccgga tttgaaaaaa aagaattcgg
     1261 cacggacgcg gatgtgtatg tgatcaatac ctgttccgtt acggaaaatg cggaccggga
     1321 atgccgttac ctggtgaggc agatccagcg gaaatcgccg gaaagtttcg tggtgatcac
     1381 cggttgctat gcccagctca agccacaggc tatcgctgcg attcccgggg tggacctcgt
     1441 tctgggcgca gcggaaaaat ttaacctcgc ggaacatatc tcccagctgg taaaaggcga
     1501 tgcggccaag atctgcagct gcgatatcga agacgtgcaa tcctttcacg aaggcgtttc
     1561 ctttaacgac cgcacgcggg cattcctgaa agtgcaggac ggctgtgatt atacctgctc
     1621 cttctgcacc attcccctgg cgcggggaaa aagccgcagc gatactattc ctcatgtact
     1681 ggacactgtg cgcgaactgg cggaaaaagg aatccgggaa gtggtcctga ccggcgtgaa
     1741 cctgggggat ttcggattct ccgctcctgc cggcaaaact tcaggaactc ccgtttacga
     1801 aaaggaaaaa acaaacgatc tgcttaaaaa gaaaccggaa aactttttcg agctcacgca
     1861 agccctggac cggatgacgg aaatgccgcg attccggatc tcttccatcg aacccaacct
     1921 gctcaccaat gatattatcg ggctggtggc cgacagccag cgcttcatgc cccatttcca
     1981 tatcccgctg caaagcggaa gcaacaagat cctcggcctg atgcgcagaa ggtaccggcg
     2041 ggaactgtac agcgaccggg ttcagctgat aaaaacacgg atgccgcatg cggccatcgg
     2101 cgccgatgtg atcgtggggt tcccgggaga aacggaagcg gattttaaag aaacggtcga
     2161 tttcctgcat tcggttgatg tttcctatct gcacgtattt acttattcgg aaagggacaa
     2221 tacccgcgcg ctggatatca aaccggaggt gcctgtttcc gtgcggaatg aacgaaataa
     2281 aattttgcgc aatctttctt ttcagaaaca gaaatacttc accgaattgc atgccgggca
     2341 aacccgcaaa gtattgttcg aaagaagccg gagccagggc atgatggaag gatataccga
     2401 caattacatc aaggtgacca cgccctaccg cgccgaatgg gcaaaccgga tcgtggattg
     2461 gcggttatag aaccagacgg gatgaccgga acctttccga atcaatattt ggccaatgaa
     2521 tgctgataaa ccgacaaata aaatttttat ttctttgcgg accatcctgt tattcattgc
     2581 cattaccagt atttcaccgg tcttcgggc
//
LOCUS       DASYCU010000528         2481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2723430, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000528 DASYCU010000000
VERSION     DASYCU010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723430"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VGM24_10655"
     CDS             <1..578
                     /locus_tag="VGM24_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY4155881.1"
                     /translation="DAAPAKQLDDINTQIEQLQKQIAVSHQQINLDNSNTAAQNRSIL
                     SEKGPLEKTVRQFQEQISKGQIVNPITGVVLTSYALKGEMAIIGKPLYKIANTDTLYL
                     RAYITGDQLPGIKLGQAVQVRIDQGEKKYKRYPGTIEWISDKSEFTPKTIQTKEERAN
                     LVYAVKVRVKNDGFLKIGMYGEVLLDGGSNK"
     gene            584..1573
                     /locus_tag="VGM24_10660"
     CDS             584..1573
                     /locus_tag="VGM24_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4155882.1"
                     /translation="MDKLDQNHLETAGATPFPIMAKGLTKTYGKSRLPAVDQVSFTVG
                     KGELFGLIGPDGAGKTSIFRILVTVLLADQGEASVAGFDVVKDYQAIRNRIGYMPGKF
                     SLYQDLTVKENLDFFASLFGTSIEENYELIRDIYDQIKPFNDRRAGKLSGGMKQKLAL
                     CCALIHKPEVLFLDEPTTGVDVVSRREFWDMLKKLKSQGISILVSTPYMDEANLCERI
                     ALIQNGKILSVDTPQNIIAAYPTRLYGIRSDNIYKLLKDIRGNDQVESSYPFGEYLHI
                     TFKNDRDQNAGELKEFGNRKGHTHFEIKQIEPGIEDSFIRLMNKGKPETGKEE"
     gene            1626..2369
                     /locus_tag="VGM24_10665"
     CDS             1626..2369
                     /locus_tag="VGM24_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4155883.1"
                     /translation="MAEKVITCKNLSKKFGDFVAVDKISFEVDQGEIFGFLGANGAGK
                     TTAMRMLCGLSFPSSGEASVAGFNVYRQQESIKRNIGYMSQKFSLYEDLSILENIRFF
                     AGVYGVPDPEIKQRSDDLVRKLGLGAEAKKRVGALPPGWKQKLAFSVAIFHRPKIVFL
                     DEPTGGVDPVTRRQFWDMIYEAAASGITIFVTTHYMDEAEYCNRITVMVDGKIEALDS
                     PKNLKEKYSAGTVEDVFYLLARKAKRTDN"
BASE COUNT          724 a          566 c          656 g          535 t
ORIGIN      
        1 ccgatgccgc cccggccaaa cagctggatg atatcaatac acagatcgaa cagttgcaaa
       61 aacagatcgc tgtttcccat cagcagatca atctcgataa ttccaatacc gccgcccaga
      121 acagaagcat actgagcgaa aaaggcccgc tggaaaagac ggtccggcaa tttcaggagc
      181 agatcagtaa aggacagatc gtcaatccca taaccggggt tgttctgacc agctatgcgt
      241 tgaagggcga aatggcgata atcggcaaac ccctgtataa aattgcaaac acagacaccc
      301 tgtacctgag ggcctatatc accggagatc agctgcccgg aataaaactg gggcaagcgg
      361 tgcaggtccg catagatcag ggagaaaaaa aatataaacg ttatccggga accattgaat
      421 ggatctccga taaatctgag ttcaccccaa aaacaattca aaccaaggag gaaagggcca
      481 acctggtgta tgccgtgaaa gtgcgggtga agaacgacgg gtttttgaag atcgggatgt
      541 acggggaagt cttattagat ggcggttcta acaaataaag cagatggata agctggatca
      601 aaaccatttg gaaacggcgg gcgccacccc ctttccgatc atggcgaagg gtttgacaaa
      661 gacctacggg aagagcaggt tgccggccgt ggatcaggta tcctttactg tggggaaggg
      721 ggagctgttt ggcctgatcg ggccggatgg agcgggcaaa acgtcgatat tccgcatact
      781 ggtgaccgtt ttactggctg atcagggaga agcttctgtc gccggctttg atgtcgtaaa
      841 ggattatcag gcgatccgca accgcatcgg ctatatgccc ggcaaatttt ccctgtacca
      901 ggatctgacc gtaaaagaaa atctggattt tttcgcaagc ctttttggta catccattga
      961 agagaactat gaattgatca gggatatcta cgaccagata aaaccgttta atgaccgccg
     1021 ggcaggaaaa ctatccggcg gcatgaaaca aaagctggcg ctctgctgcg ctttaattca
     1081 taaaccggag gtcctgttcc tggatgagcc cacaaccggc gtggatgtgg tctccagaag
     1141 ggaattctgg gatatgctca aaaaattgaa aagccagggc atcagcatcc tggtttccac
     1201 tccctacatg gacgaagcga atctgtgcga aagaatcgcc ctgatacaaa atggaaagat
     1261 cctgtccgtg gatacaccac agaacattat cgccgcctat ccgacccgtt tatatggaat
     1321 aaggtctgac aacatataca aactgctgaa ggatatccgc gggaacgacc aggtggaaag
     1381 cagctatccg ttcggagagt acctccatat tacctttaag aacgaccgcg atcagaatgc
     1441 cggggaactg aaagaattcg ggaaccggaa aggacatacc cattttgaaa taaagcagat
     1501 cgaaccgggg atcgaggata gttttatcag gctgatgaat aagggaaagc cggaaaccgg
     1561 gaaggaagaa tgagtaggaa attttaaatc acgggcagcg gcctcatcgc taaacttcta
     1621 taataatggc agaaaaagta ataacctgca aaaacctcag caaaaaattc ggcgattttg
     1681 ttgcggtcga caaaatcagc ttcgaggtgg accagggaga gatctttggt tttctgggcg
     1741 ccaacggtgc aggcaaaaca acggcgatgc ggatgctttg cggactttct ttccccagct
     1801 ccggggaagc atccgttgcg ggtttcaacg tgtaccggca acaggaaagt atcaagcgga
     1861 atatcggcta tatgagccag aaattttctc tctatgaaga cctgagcatt ctggagaaca
     1921 tccggttttt tgccggggta tatggagttc cggatccgga aattaaacag cgaagcgatg
     1981 atctggtgcg taaactcggt ttgggggccg aggcaaaaaa acgggttggt gcactgccgc
     2041 ccggatggaa gcagaaactg gccttttccg tggccatatt ccaccggcct aaaattgttt
     2101 tcctggatga acccaccgga ggggttgatc cggtcacccg caggcaattc tgggatatga
     2161 tctacgaggc agccgcttcc gggatcacca ttttcgtaac cacgcattac atggatgaag
     2221 ccgaatactg taaccggata acggtaatgg tggatggaaa aattgaagcg ctggacagcc
     2281 cgaagaacct gaaagagaaa tactccgccg gcacggtgga agatgttttc tatctgcttg
     2341 cgcggaaggc caaaagaacc gacaactgat catttattaa atgaaacaac tactggtctt
     2401 tatccgaaaa gaattttacc atgttttccg tgaccggaga accctgctga tcatgtttgg
     2461 tctgccggtt gcccagatcc t
//
LOCUS       DASYCU010000529         5751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_548988, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000529 DASYCU010000000
VERSION     DASYCU010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548988"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2677)
                     /locus_tag="VGM24_10670"
     CDS             complement(<1..2677)
                     /locus_tag="VGM24_10670"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY4155884.1"
                     /translation="MKKRLFIFLILFSALLSHGRAQMNPGNLEFVENHGQWDTAVTFR
                     ADISNGNFFLQKHGFSVLMQNAADMDALREYMHGHASRTNAGNTGSPAEKTAVSDIAP
                     IRQSGVTIGKPGTDLTMHSHLYEVEFVNSLDAVQIAGDKPLDTYNNYFIGNDSSRWAS
                     NCKIYQAITYRNIYPDIDLRYYTENGRLKYDLVVHPGGNPDDIVLRYKGVSKLAVKGR
                     QLTVYTTVGEAKETIPHTYQFSKEGNKDLDCRYVAGPNNTVRFRIDNYSRTSTIVIDP
                     TLVFSTFTGSHSDNWGYTATYDDAGNFYSGSIVLNSIRNDAGNGFLASPGAFQSKFHG
                     GDATDGTYEYDISIMKFSSNGVNRLYATYIGGKGNEQPHSMIVDNAGNLVIAGRTSSP
                     DYPVIAGSTGKVDVLKGGYDIILTKLNAAGTNLIGSRMIGGSSNDGVNIAPKYSSDIV
                     PMGQSSLRLNYGDDGRSEVILDNAGNVYLASCTRSTDFPFTANAFQKKSGGLQDGVFI
                     KTSPNLATILASTYLGGNNDDAAFALALNPVNNDIYVAGGTSSNNFPGTGNGTVVSSA
                     NKGGIDGFLSIISNDGGTLRKTSYFGTDGTEVLYGVQFDNLGNPYIMGTTTGAWPVIN
                     AKFSQAKGKQFIAKLKPDISAYIYSTTFGKGAAYPDISPTAFLVDRCQNVYVAGWGGG
                     IETEGGGSAVYHNSRTTGLTVTPDAIKKTTDGDDFYFFVMQRDATSQLYGSFFGQDNG
                     LVGDHVDGGTSRYDKQGIIYEAICANCYGMGTFPTTPGVWAPRNGTGSQGCNLAAVKI
                     SFNFAGVAADPRSMINGRYDSSGCVPLDVLFVDTLHNAKQYIWHFGDGSPDTTTTGFQ
                     ILHTYQLIGNYRVRLIAIDSSTCNVSDTAYLNIRVR"
     gene            complement(2702..4816)
                     /gene="recG"
                     /locus_tag="VGM24_10675"
     CDS             complement(2702..4816)
                     /gene="recG"
                     /locus_tag="VGM24_10675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY4155885.1"
                     /translation="MSRISPEILASPIEYLKGVGPHRAEMLRKELQIDCFRDLLELYP
                     YRHIDKSRISPISEISSNTEFIQVAGILGPLEILGSGRGRRLTAELIDSTGALPLVWF
                     QSISWIQKSLSPGLRYLVFGRVSFFNGERQITHPEIEIFPAEKPAERFSLQPVYPSTE
                     KLKTRGLGGRQLAKFTQQLLSQLREEDVPEFLPRQLLETLKLTPRIQAYRNVHYPAGL
                     QDYQSALNRLKFDELFLNQVRTGMIKTERQKHSRGAVFKQVGPFFNEFYSKVLPFELT
                     AAQKRVLKEIRKDVATGRQMNRLLQGDVGSGKTMVALLAMLLAADNGFQSCLLAPTEI
                     LSKQHADRFRELLKGLPLRISLLTGTVKSRTRKKILEELNSGELNILIGTHAILEDPV
                     RFHNLGLAIIDEQHRFGVAQRARLWQKAAIPPHMLVMTATPIPRTLAMTAYNDLDYSV
                     IDELPPGRQPVRTVQRYEYDRPKVMDFIKEEIKKGRQAYIIFPLIEESEKLDYENLLK
                     GYENVKAYFPEPPYYISMVHGRQKAEVKETNMSRFVTADTQIMVATTVIEVGVDIPNA
                     SVMVVESAEKFGLSQLHQLRGRVGRGNAHSFCILITGNKLGSDARERLKIMCETSDGF
                     RIAEKDMELRGPGDIAGTRQSGLLDFKLADIVRDKNILQIAVQSAEKILEEDPGLSLA
                     DHTPLRNQLMLEKGKTAWSRIS"
     gene            4982..5584
                     /locus_tag="VGM24_10680"
     CDS             4982..5584
                     /locus_tag="VGM24_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY4155886.1"
                     /translation="MPEFKRIAFFDFDGTITTRDTLPEIIRFQKGTLSLYTGLLRHFP
                     LLLAYKLKIVSNDRAKQKVLRYFFGGMNAGLFQQNCDLFAEERLSRLIRPAALEEINK
                     LKKEKTEVVIVSASAGNWIRKWSDSLELELVSTSLEIKNGLITGRIAGKNCHGEEKVR
                     RIREKWNLNEFDEVYAYGDTEGDRPMLALATKSFFRPFRG"
     gene            complement(5565..>5751)
                     /locus_tag="VGM24_10685"
     CDS             complement(5565..>5751)
                     /locus_tag="VGM24_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4155887.1"
                     /translation="TRNRILYMRRNSPFFQLLVFTLFFSLFSMPKSIISYLLKRQFRQ
                     LTWFLKGIGYNLTRGRA"
BASE COUNT         1330 a         1518 c         1452 g         1451 t
ORIGIN      
        1 tacgcacccg gatattcagg taggccgtat cgcttacgtt gcaggtgctg gaatcaatag
       61 cgatcagccg cacgcggtaa tttccgatga gctggtaagt atgcaggatc tgaaacccgg
      121 tggttgtggt gtccggcgaa ccgtctccaa aatgccagat gtattgtttt gcgttgtgaa
      181 gggtgtcgac aaataacacg tcgagcggaa cacaacccga actgtcgtag cgtccgttga
      241 tcatggacct gggatccgcc gccacaccgg caaaattaaa agagatcttc acggcggcga
      301 ggttgcaacc ctgtgagccg gttccgttcc ggggagccca gactcccggt gtggtcggga
      361 aagttcccat gccatagcaa ttggcgcaga tggcttcata aatgattccc tgtttatcat
      421 agcggctggt gcctccgtcc acatgatctc cgaccaatcc gttgtcctgg ccaaaaaaac
      481 ttccatagag ctggcttgtc gcatcgcgct gcatcacaaa gaaataaaaa tcatccccgt
      541 cggttgtttt cttgatggca tccggggtaa cggtaaggcc tgttgttctg gaattgtgat
      601 aaactgccga accgccgcct tccgtttcga ttccgcctcc ccatccggct acataaacat
      661 tctgacaacg gtcaaccaga aaagcggtgg gcgaaatatc gggatatgcc gcccccttgc
      721 cgaaagtggt cgaatatata taggcggaaa tatcgggttt taattttgca atgaattgtt
      781 tgccctttgc ctggctgaat ttggcattga tcacgggcca ggcgccggtg gtggtcccca
      841 tgatataggg attgcccagg ttgtcgaatt gcacaccata caacacttcc gttccgtcgg
      901 tgccgaaata ggatgtcttc cgcagggttc ccccgtcgtt gctgataatg gagagaaagc
      961 catcgatgcc gcctttattg gcggaagaga ccaccgttcc gtttcccgta cccgggaaat
     1021 tgttgctgga cgtgccgccc gcgacataga tatcgttatt gaccggattt aatgccaggg
     1081 caaatgcggc atcgtcattg tttccgccaa gataagtgct cgccagtatg gtggccaggt
     1141 tcgggctggt cttaatgaaa acgccatcct gcaatccccc gctttttttc tgaaaggcat
     1201 tcgccgtgaa cggaaaatcc gtggagcggg tgcaggaggc gagatataca ttcccggcat
     1261 tgtccagtat cacttcgctg cggccgtcat ctccgtaatt caggcggagc gaactctgtc
     1321 ccatgggaac aatatcggaa gaatatttcg gcgcaatatt caccccgtcg ttcgaactgc
     1381 ccccgatcat ccgggatccg atcaggttgg tgcctgccgc gttgagcttg gtcaggatga
     1441 tatcgtaccc gcctttcaaa acgtctacct tgccggtaga gcccgctatg accggataat
     1501 ccggagaact ggtcctgccg gcaatgacca ggttacccgc attgtcgacg atcatactgt
     1561 gcggctgttc attccccttt ccgccgatat aggtcgcata tagccggtta acgccgttgc
     1621 tgctgaattt cataatggag atatcgtatt cataagttcc atcggtggcg tcgccgccat
     1681 gaaatttgga ctggaaagcg cccgggctcg ccagaaaacc attgcccgcg tcatttctga
     1741 tggagttcag tacgatactg ccggaataaa aatttccggc atcatcataa gtggccgtat
     1801 atccccagtt gtcggaatgg ctgccggtaa aagttgaaaa gaccagggtc ggatcaatca
     1861 cgatggtgga tgtccgggaa taattatcga tccggaaacg aacggtatta ttcggaccgg
     1921 caacataccg gcaatccagg tccttgttgc cttcttttga gaattgatag gtatgcggaa
     1981 tggtttcctt cgcttctcca accgtggtat acacggtcag ctgccgccct tttaccgcga
     2041 gtttactcac tcctttatat cgcagcacga tatcatccgg atttccgccg ggatgaacga
     2101 ccaggtcata tttcaggcgc ccgttttccg tgtaataacg cagatcgata tccggataaa
     2161 tattcctgta ggttatagcc tggtatattt tgcaattgga agcccatcgg gaagaatcat
     2221 tgcctataaa ataattgtta taggtatcga gcggtttatc tccggcaatc tgcacggcat
     2281 ccaggctgtt cacaaattca acttcataga gatgggaatg catggtcagg tccgtcccgg
     2341 gtttcccgat ggtaacgccg ctttgtctga tcggggcaat atcgctgacg gccgtttttt
     2401 ccgcaggcga acccgtattg cccgcattgg tccgggaagc atgcccgtgc atatactccc
     2461 gcagcgcatc catgtctgcc gcattttgca tcagcacgga aaacccatgc ttttgcaaaa
     2521 aaaaatttcc attggaaata tctgcccgga aggtcaccgc cgtatcccac tgcccgtgat
     2581 tttcaacaaa ctccagattg cccgggttca tttgagcccg gccatggctt agcagcgccg
     2641 aaaacaaaat taaaaagata aataaccgtt ttttcaatcc tatttttttt gaccgaatca
     2701 ttcaggagat cctgctccag gctgtctttc ctttttccag catcagctga ttgcgcaggg
     2761 gggtatgatc agccaaactg agcccgggat cttcttccaa tatcttttcc gcagactgaa
     2821 ccgcgatttg caggatattc ttatctctta cgatatctgc cagtttaaaa tccagtaatc
     2881 cgctttgccg ggttccggcg atgtcccccg ggccccgaag ctccatatct ttttctgcaa
     2941 tccggaatcc gtcggaggtc tcacacataa tctttaaacg ctcccgggca tccgatccga
     3001 gtttatttcc tgttattaag atgcaaaaac tatgggcatt tccccggccc acccttcccc
     3061 gcaactgatg caattgggaa agtccgaatt tttcagcact ttccaccacc atcacgctgg
     3121 cattcggtat gtccaccccc acttcaatta cagtagtggc caccatgatc tgcgtatccg
     3181 cggtcacaaa ccggctcata ttggtctcct tcacttcggc cttctggcgc ccatgcacca
     3241 tgctgatata gtagggcggt tccggaaagt acgcttttac attttcataa cccttcagca
     3301 gattttcgta atcgagcttt tcggactcct cgatcagggg aaagataata taagcctgcc
     3361 gccctttttt aatttcttcc ttaatgaaat ccatcacttt aggcctgtca tactcataac
     3421 gctgcacggt tctgacgggt tgccttccgg gagggagttc gtcaattaca ctatagtcca
     3481 ggtcgttata ggcggtcatg gccagggtcc ggggaatggg cgttgcggtc ataaccagca
     3541 tatggggcgg aatggctgct ttttgccata gtctggccct ctgggccacc ccgaaccggt
     3601 gctgttcatc tataatggca agccccaggt tatggaaccg gacgggatcc tccagaatgg
     3661 catgtgtgcc gataaggata ttcaattccc ccgaattcag ctcctccagt atctttttcc
     3721 gggtcctgga tttcaccgtg cccgttaaca gggagatcct caggggaagc cctttgagca
     3781 attcgcgaaa ccggtccgcg tgttgtttgc taaggatttc cgtcggggcc agtaaacagc
     3841 tttgaaaccc gttgtccgcc gccagcagca tggccaggag cgccaccatg gttttcccgc
     3901 tccccacatc cccctgtaaa agccggttca tctgcctgcc cgtggccacg tccttgcgga
     3961 tctctttcag gacccgtttt tgggcagccg tgagttcaaa gggaaggacc tttgaataga
     4021 actcgttgaa aaacggaccg acctgcttaa atacagcccc cctggaatgt ttctgcctct
     4081 ccgttttaat catccccgtc cggacctggt tcaggaacaa ttcgtcgaat ttgagccggt
     4141 tcagagcact ctggtaatcc tggagaccgg ccgggtaatg tacgttccgg taagcctgta
     4201 tacggggcgt cagcttcagc gtttccagaa gctgccgggg taaaaattcg ggaacgtcct
     4261 cttccctgag ctgggaaagc agctgctgcg tgaatttagc cagctgacgg ccgcccagac
     4321 cccgggtttt cagtttttcc gtgctgggat acaccggttg aaggctaaag cgctctgccg
     4381 gtttttcggc cgggaagatc tctatttccg ggtgggtgat ctgccgctcc ccgttaaaaa
     4441 agctgaccct tccgaagacc aggtaccgca accccgggct cagggatttt tgaatccagg
     4501 agatgctctg gaaccaaacg aggggaaggg ccccggtgga atctattaat tccgcggtca
     4561 gccttctgcc ccggcccgat cccagaattt ccaggggtcc caaaatgccg gctacctgaa
     4621 tgaattcggt attggaagaa atttcgctga tgggactgat gcgcgatttg tcgatatgcc
     4681 ggtacggata cagttccagc aggtccctga aacaatcaat ctgaagttcc ttgcgaagca
     4741 tttctgcccg gtgcgggccc actcccttca ggtactcgat cgggcttgct aatatctccg
     4801 gggaaatcct gctcatgagc gtggacaaat ttagttgaat tcccgttggg acgctgcttc
     4861 cgggtattga aacgggaagg gcgccaacgc gcttcttccc atctcggaaa atttaaggat
     4921 cttgtcgcaa aatatgaccc gcggaagata tcccggattt tcaatttaaa aagataaagt
     4981 tgtgcctgaa ttcaaacgaa ttgctttttt tgattttgac ggaaccatca ctacccggga
     5041 tacgctgccg gagatcatcc gcttccagaa aggaacgctt tccctgtata cgggactctt
     5101 gcggcatttc cctttgctgc tggcctacaa attgaaaata gtctccaatg accgggccaa
     5161 gcaaaaggtc ctccggtatt ttttcggggg aatgaacgcc ggcctgtttc agcagaattg
     5221 cgatctgttt gccgaagaaa gactctcgcg gctgatccgc ccggcggcgc tggaggagat
     5281 caataaatta aaaaaagaaa aaacggaagt ggtcattgtc tcggcctcgg ccggcaactg
     5341 gatccgcaag tggagtgatt ccctcgagct ggaactggtt tccacttcac tcgaaataaa
     5401 gaacgggctg atcaccggcc gcattgccgg caaaaattgt cacggagagg aaaaggtccg
     5461 gcgcatccgc gaaaaatgga accttaatga atttgatgaa gtgtatgcgt atggagatac
     5521 ggaaggagac cggcccatgc tggccctggc aacgaaatcc ttcttcaggc ccttccgcgg
     5581 gtaagattat agcctattcc ctttaaaaac caggtcagct gccggaactg tcttttcagc
     5641 agataggaga tgatggattt gggcatggaa aataaactga agaacaaagt aaataccagc
     5701 agctgaaaaa aaggactgtt gcgtcgcatg tataagatcc ggttgcgggt a
//
LOCUS       DASYCU010000530         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2578779, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000530 DASYCU010000000
VERSION     DASYCU010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578779"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..374)
                     /locus_tag="VGM24_10690"
     CDS             complement(6..374)
                     /locus_tag="VGM24_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY4155888.1"
                     /translation="MPSPKKTVVLGASDNPERYSNLAIRKLHAHGHPVIAIGKKKTSV
                     DGVPVQDQAVFTPGVDTVTLYLNPKNQASYYDYIFSLNPKRIIFNPGAENAELEKLAE
                     ERNIKTQEACTLVLLSTGQF"
     gene            complement(414..1169)
                     /locus_tag="VGM24_10695"
     CDS             complement(414..1169)
                     /locus_tag="VGM24_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY4155889.1"
                     /translation="MKAFLTICSSLCFLSAFSQFKESFYALDSNWKQTTEKKASYLLW
                     IHEDSARNWEYSYYHMWGPLIKLETYRDHQGTQRNGLACYYYTTGNLDSLGHYKDGKK
                     EGKFYKYAFLPQDSLRKTMEYTYANDSLIKTVDHRRDGRDKNDADTAGNKEARFPGGS
                     SQWMQYLQKNLRYPDRAASNEIQGSVRVSFTIDGDGNVLEPIVSKSVEYSLDREALRI
                     IANSGKWEPATRISSPVESDKVQPVIFRLAVSK"
     gene            1297..3504
                     /locus_tag="VGM24_10700"
     CDS             1297..3504
                     /locus_tag="VGM24_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4155890.1"
                     /translation="MSGTDLSNADFQLALQFVNQTAQHIFLTGKAGTGKTTFLKYITQ
                     NCSKKMAVVAPTGVAAMNAGGVTMHSFFQLPFNPFVPGEGNYWDESGRVVNEQTLLKN
                     IHLSAAKKELLQELDLLVIDEVSMLRADMLDAVDAVLRFCRKKTTPFGGIQVLFIGDL
                     YQLPPVINQTEWEILKSHYQSPFFFDARVLKNCSMVYISLRKIYRQKDSLFIDLLNSI
                     RNNVVKEEDLDRLHRRYEPDFQPAYGEQFIILTTHNARADAINQQELSKLPGSRYSFE
                     AETTGDFDPRSVTTDSQLFLKKGAQVMFVKNDKGENRRYFNGKIGFVSRISSVEIGVE
                     FPGEPGVLLLEKETWKNIRYHYNREKGTVEEETIGSFTQYPVRLAWAITIHKSQGLTF
                     EKAIIDAGASFAPGQVYVALSRLTSLEGLVLYSRIHPRSIRTDPEVIRFSQSEEASTG
                     LQERLQQEQAGYVLRLIEEAFQWEKLLSVFRIHYDRYGNSLVPVNDLSAAWAKQILGR
                     LEEESVVCEKFGKQLTSLLAGAKEDGYTTLQPRIEAACNYFIKVIDLLMASVEKHKPG
                     SKKTTWARKYAKELAGLEKTLHFKKDQLDQALRMGEGLAKGMDVNSLFDITRPPQPVL
                     RLKTEKTTGKLKKGETQRISLDLFKENKTVSEIARLRGLAVMTVENHLAAFVRTGEIE
                     IHELVPEEKIAKIVEAVRETGERFPGLVKNILGESFSYLEIKAVMNSMEKALI"
     gene            complement(3521..>4535)
                     /locus_tag="VGM24_10705"
     CDS             complement(3521..>4535)
                     /locus_tag="VGM24_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEY4155891.1"
                     /translation="PFHKVCGEYVSMESWDFLTKLGLPLDEWNLPRINRLRISAPSGR
                     SLREALPLGGFGVSRFKLDSELAGLAKKTGVNVYENTKVSGVEYKAELFRIISSSGLF
                     MAHTVCGSYGKRSNLDVKWKRSFIHSSRIKNYVGIKYHVRANFPEQEIALHNFAGGYC
                     GISKIEEDRYCLCYLTRSENLKKSGQSIPEMEERILKKNPRLRELFNQVEFLYEQPLT
                     IAQISFAKKTQVEDHVLLVGDAAGMITPLCGNGMSMALHGSKIAASCLHAFFRKEYDR
                     AGMEADYQRQWNRVFAKRLGTGRFLQYFFGNRFLSGMLISILKPFPFLVRRLIRKTHG
                     EPF"
BASE COUNT         1159 a         1095 c         1142 g         1139 t
ORIGIN      
        1 cgcatttaaa actgtcctgt ggacagcagg accagcgtgc aggcttcctg cgttttaatg
       61 tttcgttctt ccgcaagctt ttccagttct gcattttcgg cgcccggatt gaatatgatc
      121 cttttgggat ttaaggagaa gatataatcg tagtaggagg cctggttttt tggattcaga
      181 taaagggtaa cggtatctac tcccggtgtg aaaacggcct ggtcctgaac cggcacacca
      241 tcaacggatg tctttttttt gccgatagcg attacgggat gaccatgcgc atgtaatttt
      301 cttattgcca ggttgctgta tcgctcgggg ttatccgacg caccgagaac aacggttttt
      361 ttgggagaag gcatagataa taaacaataa aatgcgccaa tagttcaatc gctttatttc
      421 gaaacggcga ggcggaaaat cactggctgg accttatccg attcgacggg gctgctgatc
      481 cgggtggcag gttcccattt accggagttc gcaatgatgc ggagggcttc ccggtcaagg
      541 gagtattcca cggatttgga aacgatgggt tccagcacat tcccatcccc gtctatagtg
      601 aatgataccc ggacagagcc ctggatctca ttggaggctg cgcggtcggg ataccgaaga
      661 tttttttgta aatactgcat ccattgcgaa cttcctccgg gaaaccgggc ttccttattt
      721 ccggccgtat ccgcatcatt cttatctctg ccgtcccggc ggtggtccac ggttttgatc
      781 aggctgtcat ttgcataggt atattccatg gttttccgga ggctgtcctg cggcaggaaa
      841 gcatatttat aaaattttcc ttcttttttt ccatctttat agtggcccag ggaatccaga
      901 ttgccggtcg tatagtaata gcaggccagc ccgttgcgtt gcgtcccctg gtgatcccgg
      961 taggtttcca gtttgatcag aggcccccac atatgataat aactgtactc ccagttgcgc
     1021 gctgaatctt catggatcca caacagatag ctggcttttt tttccgttgt ttgtttccag
     1081 tttgaatcca gcgcataaaa agattcctta aactgtgaaa aggcagacag gaagcaaagg
     1141 ctggagcaaa tggtcagaaa ggctttcatg ggaaccaatt taaaacattt tgacagaaaa
     1201 ccgcccaact gttaacctcc ccacctaatc aaaaggtata ccggctctga agcattttca
     1261 cctgcccgca ttccgtttca tttcatatat tgccacatgt ccggaacaga cctgtccaat
     1321 gccgattttc aactggccct gcagtttgta aatcaaaccg cccagcatat ttttcttacc
     1381 gggaaagccg gaacgggcaa aacaactttt ctgaaataca tcacacagaa ttgcagcaag
     1441 aaaatggccg ttgttgcgcc aacgggtgtg gccgccatga atgccggcgg ggttacgatg
     1501 cattctttct ttcagctgcc attcaatccg ttcgtgcctg gcgaaggaaa ttattgggat
     1561 gaaagcggcc gggtggtcaa cgagcaaacc ctgctgaaaa atatccatct gtctgccgca
     1621 aagaaagagc tattgcagga actggacctg ctggtgatcg atgaggtcag catgctgcgg
     1681 gccgacatgc tcgatgccgt ggatgccgta ctccgctttt gccggaaaaa gaccacgccg
     1741 tttgggggca tccaggttct ttttatcgga gacctgtatc agcttcctcc cgttatcaat
     1801 caaacggaat gggaaatact gaagagccat tatcagagtc cgttcttttt tgatgcccgg
     1861 gttctgaaga attgctccat ggtttatatt tccctcagga aaatataccg ccagaaagat
     1921 tccctgttca ttgatctcct gaacagcatc cggaataatg tggtcaagga ggaggacctg
     1981 gacaggctgc atcggcgtta tgaaccggat tttcaaccgg cctacggaga acagtttatc
     2041 atcctaacca cacataatgc ccgggcggat gccatcaatc agcaggagct ctctaaatta
     2101 cccggatccc gctattcttt tgaggccgaa acgacgggag attttgatcc gagatcggta
     2161 accaccgaca gccagctgtt tctgaaaaaa ggggcacagg tgatgtttgt gaaaaacgat
     2221 aagggcgaaa accgccgtta tttcaatgga aagattgggt tcgtcagccg gatttcttct
     2281 gtggagatcg gcgtagagtt cccgggagaa ccgggagtat tgctgctgga aaaggaaacc
     2341 tggaaaaata tccgctatca ttataaccgg gagaagggaa cggtggaaga agaaaccatt
     2401 ggaagtttca ctcagtaccc ggtccggctg gcctgggcaa tcaccattca taaaagccag
     2461 ggattgactt ttgaaaaagc gatcatcgat gccggagcat ccttcgcgcc gggccaggta
     2521 tatgttgccc tgagcaggct gacttcttta gaagggctgg tgctatactc ccgtattcat
     2581 ccccgcagta tccggaccga cccggaggtg atccgcttct cccaatcgga agaggcatcc
     2641 accggtttgc aggaacgact ccagcaggaa caggccggat acgtgcttcg gttaattgaa
     2701 gaagcatttc aatgggaaaa gctcctgtct gttttccgga ttcattatga tcgctacggc
     2761 aattccctgg ttccggtaaa tgatttatct gctgcatggg caaaacaaat cctcggcaga
     2821 ttggaagagg agtctgtcgt gtgtgaaaaa ttcggcaaac agctgaccag cctgctggcc
     2881 ggagcaaaag aagatgggta tacaaccctg caaccccgga tcgaggcggc ctgtaattat
     2941 tttatcaaag tgatagacct gttgatggct tccgtggaaa aacacaaacc cggatcaaag
     3001 aagaccacct gggccagaaa gtatgcgaag gaattggcgg gtttggaaaa aacccttcat
     3061 ttcaaaaagg atcagcttga tcaggccctc cggatgggcg aaggactggc taaaggaatg
     3121 gatgtgaatt cgctttttga tattacgcgg ccgcctcaac cggttctgcg gctaaaaaca
     3181 gaaaagacca cagggaaatt aaaaaaaggc gaaacgcagc ggatcagcct ggacctgttt
     3241 aaagaaaata agaccgtttc ggaaatagcc aggcttcgcg gactggccgt tatgaccgtc
     3301 gaaaatcacc tcgctgcatt tgtaagaacc ggagaaattg aaattcatga attggtgccg
     3361 gaagaaaaaa tagccaaaat tgtggaagct gtccgggaga ccggcgaaag gtttcccggc
     3421 ctggttaaaa atatactcgg agagtcgttt tcttacctcg agataaaggc agttatgaac
     3481 tccatggaaa aggcgctgat ctgacggcgg cagctaacag ctagaacggt tccccgtgtg
     3541 ttttccggat cagcctgcgg acgaggaagg ggaagggttt taatatgctg atcagcatcc
     3601 ctgacaaaaa ccgattcccg aaaaaatatt gcaggaaacg ccctgtgccc aggcgttttg
     3661 cgaaaacgcg gttccattgc cgctgatagt ccgcttccat ccccgcccgg tcgtactcct
     3721 tcctgaaaaa ggcatgaagg caggaagccg ctatcttgga tccgtgcagc gccatgctca
     3781 tcccgtttcc acaaagggga gtgatcatgc cggctgcatc tcccactaac aatacatgat
     3841 cttccacctg cgtttttttc gcaaaactga tctgtgcgat cgtcaggggt tgctcatata
     3901 gaaattcaac ctggttaaac agctcgcgaa gccttgggtt ttttttgagg atcctttcct
     3961 ccatttccgg aatcgactgt ccgctttttt ttaaattttc agaccgggtc agatagcata
     4021 agcaatagcg gtcttcctcg attttggaaa tgccgcaata gccgcctgcg aaattgtgaa
     4081 gggcgatctc ctgttccgga aaattggcac ggacatgata tttaatgccc acataatttt
     4141 ttatccggga ggaatgaata aaagaccgtt tccattttac gtccaggtta cttctttttc
     4201 cataactgcc gcagacggta tgggccatga ataaaccgga agaactgatg atccggaaaa
     4261 gctccgcctt gtattcgact cccgaaacct ttgtgttttc atatacattg acccccgttt
     4321 tctttgccag gccggccagt tcggaatcca gtttaaagcg gctgacgcca aaaccgccca
     4381 ggggcagggc ctcccgtagg gatctgccgc tgggcgcgct gatgcggagc cggttaatcc
     4441 ggggcaggtt ccattcgtcc agcggcaaac caagcttcgt taaaaagtcc cagctttcca
     4501 tgctgacgta ttctccgcaa accttatgaa aaggg
//
LOCUS       DASYCU010000531         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3738640, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000531 DASYCU010000000
VERSION     DASYCU010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738640"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VGM24_10710"
     CDS             complement(<1..372)
                     /locus_tag="VGM24_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155892.1"
                     /translation="MEKRISELFKKYLSNHCTVEEMDEFFHYIRQSRSDEPLRKLIRD
                     TYQAIRNEPETYVDHTGELFIPQAGEGAPPVFPDAVRIPGKKKLARWMVAAGILSGIS
                     IGALYFSKTSSAALTENARLAL"
     gene            complement(446..976)
                     /locus_tag="VGM24_10715"
     CDS             complement(446..976)
                     /locus_tag="VGM24_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEY4155893.1"
                     /translation="MEYLAGKEGLEAEFSHMVKVHAQNLHLLALRVTKCEQLAKDVVQ
                     DVFLKLWEHRNQFHQIENIEAWLYRVTENKLIDHLRKTAADKRLKEISWSRIRATNNE
                     TEQRLTARESRELISRAIENLPAQRKIIYRLNREQLLSYRQIAEELQISPHTVKNQLS
                     SALHYLRSCLKSIRLF"
     gene            1106..1738
                     /gene="yihA"
                     /locus_tag="VGM24_10720"
     CDS             1106..1738
                     /gene="yihA"
                     /locus_tag="VGM24_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY4155894.1"
                     /translation="MEIRSAEYVISSPDYQSCPAADRPEYAFIGRSNVGKSSLINMIC
                     DIDRLAKTSAAPGKTQLINHFAIESISPSRVVSKWYLVDLPGYGFAKVSQAQRRKWEK
                     MIEDYLRKRETLIHVFMLVDSRHNPQKLDLDFANNLGKWEIPFTLVFTKADKEKQRTV
                     QANVRAFLNKMLESWQFLPRHFISSAVKKTGRENLLRLIGEMNEELKQTK"
     gene            complement(1767..2240)
                     /locus_tag="VGM24_10725"
     CDS             complement(1767..2240)
                     /locus_tag="VGM24_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HEY4155895.1"
                     /translation="MLWRKFNGDLIELPIAHAVENAIKRETREGFHLKVCIGTDSQVK
                     GAETEFATVIVFLREGHGGFMFIHNEKTKTRYSIKERMLVEVARSIEIAYELCPLFTK
                     YNVDMEVHADINTNPGFKSNDALKEAMGYILGMGFAFKAKPEAFASSSCANKVVN"
     gene            complement(2301..>2881)
                     /locus_tag="VGM24_10730"
     CDS             complement(2301..>2881)
                     /locus_tag="VGM24_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155896.1"
                     /translation="IGSFVWGDDTPAWVFTNNLSNNPAFIAASATHQIGHTLGLQHQS
                     LYDSYGIMISELSGGENNILTNEAPLMGIPFYKPADWSTGTSSTGPGDLQNDTARIAG
                     APNNIGYRKNTGDLPKTEPPVKIGNQASGSIDLSSSGDYAYRLFDISGRLLTQGNLRK
                     GWNQIPAQSMPNAVLVLQWYGESGSGSQKIIR"
BASE COUNT          696 a          692 c          711 g          782 t
ORIGIN      
        1 caacgccagg cgggcgtttt ccgttagtgc cgcagaactg gttttgctga aatacaaagc
       61 gccaatggaa atcccggaaa ggattccggc tgcgaccatc catcttgcca atttcttctt
      121 cccgggtatc cggacggcat ccgggaagac cggtggcgcg ccttccccag cttgcgggat
      181 aaacagttcc ccggtgtggt ccacataggt ttccggctca ttccggatgg cctgataggt
      241 atcccggatg agcttgcgca ggggctcatc cgagcgggat tgacggatat aatgaaagaa
      301 ttcatccatt tcttccaccg tgcaatgatt tgaaaggtat tttttaaaca gttcgcttat
      361 tcttttttcc atatgcaggc ttagtccatg aatagacgaa cgggaggagc gatgttccta
      421 ttcccctcaa aaaaaatgtt ctttttcaga aaagccggat gcttttgagg caggagcgca
      481 ggtaatgcaa agcgctggac aactggtttt ttaccgtgtg cggtgaaatt tgtaattctt
      541 cggcgatctg ccggtagctg agtaattgtt cgcggttcag ccggtatatg attttgcgct
      601 gggcgggtaa attttctatc gcgcgggaaa tcagctccct ggattcccgt gccgtaagtc
      661 tttgttcggt ttcattattc gttgcccgga tccggctcca ggaaatttct ttcaaacgtt
      721 tatctgccgc agttttgcga agatggtcga tcagtttgtt ttcggttacg cgatacagcc
      781 aggcttctat attttctatc tggtgaaact gattccggtg ttcccataat ttcaaaaaca
      841 catcctggac cacatctttg gcaagctgtt cgcatttcgt aacacgtagg gccagcagat
      901 gcaggttttg cgcgtgcact ttgaccatat gggaaaattc tgcctccaac ccttccttgc
      961 cggcgagata ttccataagt tcagtcgttt tgtcattctc aatttagtaa aattgtttat
     1021 aaggccggtt ttatttttat tatgaagatg aacaggcgcc ttcatccggt tgccccgaag
     1081 aacttttgaa atattaactt gcagtatgga gatcagatcc gcggaatacg tcatcagcag
     1141 tccggattat caaagctgtc cggcggccga ccggcccgaa tatgcattca tcggacggag
     1201 taatgtgggt aaatcttccc tgatcaatat gatctgcgac atagaccggc tggcaaaaac
     1261 ttccgccgcg ccgggcaaga cccagctgat caatcatttt gccatagaaa gcatcagccc
     1321 gtcccgggtt gtgtcaaaat ggtacctggt ggacctgccg gggtacggct tcgcgaaggt
     1381 ttcgcaggcc cagcgcagga aatgggaaaa aatgatcgaa gattacctga gaaaaaggga
     1441 aaccctgatc catgttttta tgctcgtcga cagccggcac aatccccaaa aactcgatct
     1501 ggattttgcc aataacctgg gcaaatggga aattcctttt accctggttt ttacaaaggc
     1561 cgataaggaa aaacagcgga cagttcaggc gaatgtcagg gcgtttctaa ataaaatgct
     1621 ggaaagctgg caattcttgc cacgacattt tatcagcagc gccgtaaaaa aaacgggtag
     1681 ggaaaacctg ctccggctga tcggggaaat gaatgaagag ttgaaacaaa caaaataaag
     1741 ttgtttcccg cggatcggcc tgcggatcag ttcaccacct tgtttgcgca gctgctgctg
     1801 gcgaaggctt ccggtttggc cttgaatgcg aatcccattc ccaggatata acccatcgct
     1861 tctttcaaag catcgttgct tttgaatccc ggattggtgt tgatgtctgc atgaacttcc
     1921 atatccacat tgtatttcgt gaacagggga caaagttcat aagcgatttc aatgctccgg
     1981 gccacttcca ccagcatacg ctccttaatg ctgtaacgtg tttttgtttt ttcattgtga
     2041 atgaacataa aaccgccatg accttcccgg agaaatacaa tcacggtcgc aaactcggtt
     2101 tctgcgccct taacctggga atcggttccg atgcaaactt ttaaatgaaa accctcccgg
     2161 gtttcgcgct ttattgcgtt ttccaccgcg tgggcaatcg gtaattctat gagatcaccg
     2221 ttaaattttc gccagagcat atattaatgg gtttttcgaa tgtagcgaaa aatcggctgt
     2281 tcagaaaaaa tagttgatta ctaccggatg attttttggg atccggatcc ggattcgcca
     2341 taccattgca ggaccagcac ggcattgggc atggattgtg ccgggatctg attccatcct
     2401 ttccggaggt ttccctgcgt gagtaaacgc ccgctgatgt cgaacaggcg gtatgcgtaa
     2461 tcgccggaac tgctcaaatc gatggagccg gatgcctggt tcccgatttt taccggaggc
     2521 tctgttttag gcaaatcgcc cgtatttttc cggtagccaa tattattcgg cgctcctgca
     2581 atccgcgcgg tatcgttttg aagatccccg ggccccgtag atgaagtccc ggtcgaccag
     2641 tccgccggtt tataaaaagg tatgcccatc aacggcgctt cattggttag tatattattc
     2701 tccccgccgc tcagttcgga gatcatgatg ccgtatgaat cataaaggct ctggtgctgc
     2761 agtcccagcg tatgcccgat ctgatgtgta gccgaagccg caatgaaggc cgggttgttg
     2821 gataaattat ttgtgaaaac ccaggccggt gtatcatcgc cccaaacaaa ggaaccgatg
     2881 g
//
LOCUS       DASYCU010000532         4867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3449638, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000532 DASYCU010000000
VERSION     DASYCU010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449638"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..498)
                     /locus_tag="VGM24_10735"
     CDS             complement(115..498)
                     /locus_tag="VGM24_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY4155897.1"
                     /translation="MGTTKYIKPTESELEILRILWERNTASVREVHEELSKTKEAGYT
                     TTLKLMQIMNEKGLVGRDDSVKTHIYHPVVSREKTQKHLIGKMISTLFGGSSTELVLH
                     ALGNHKASPEELEEIQLLLNNLKKQ"
     gene            618..1523
                     /locus_tag="VGM24_10740"
     CDS             618..1523
                     /locus_tag="VGM24_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEY4155898.1"
                     /translation="MANQRDSGYYAGKYKIPEGKPVSLKELPTDDKDKQVSKEEGEDL
                     LKSDILKLTELQERLYSSNRYSVLMVIQAMDAAGKDSVVKHVMSGLNPQGVVVTSFKT
                     PTHQELEHDYLWRHYLALPARGVVGIFNRSHYENVLVTRVHPEFILNENLPGITKVED
                     IDHSFWERRFKQINHFEKTLADNGMVILKFHLHVSKKEQKKRFMERIEDPSKNWKFSS
                     ADLKERALWKDYRKAYEDMLENTSKDNAPWFSVPADDKWFTRLVIANIIVHELSSLDL
                     HYPELSSARKTDLLKSKADLLNEDL"
     gene            complement(1562..3763)
                     /locus_tag="VGM24_10745"
     CDS             complement(1562..3763)
                     /locus_tag="VGM24_10745"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY4155899.1"
                     /translation="MDKLIYLVPLMGIIGLLYTFVKFKWVSRQEEGTERMKEISRHIA
                     EGAMAFLKAEWKILGYFVVVVALLLGFMSITNPHSHWAISISFILGAILSATAGYIGM
                     RVATKANVRTAHAARTSLSRALKVSFTGGSVMGLGVAGLAVLGLGGLFIILKAVFAPD
                     AGVNSEEMRLTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGQETKADDAFGGMSPILLP
                     MLIAGVGILFSIVATLFVKISETASIRTGTVQRALNMGNWGSIILTAIASVGLVFYVL
                     PETMELRGIEFSKWGVLGAIGVGLLVGTLMSLITEYYTAIGRRPVLSIIRQSSTGHAT
                     NVIGGLAIGMESTCLPILVLAGGIWGSFACAGLYGVAIAAAGMMATTAMQLAIDAFGP
                     IADNAGGIAEMSELPKEVREKTDILDAVGNTTAATGKGFAIASAALTSLALFAAFVGV
                     AGISGIDIYKAKVLASLFVGGMIPFVFSSLAIRAVGQAAMAMVEEVRRQFRTIPGIME
                     GTGKPEYDKCVAISTDASIKKMMAPGAIAILSPLVIGFLLGPEALGGFLAGATVSGVL
                     MGMFQNNAGGAWDNAKKSFEKGVEINGEIHYKKSEPHKASVTGDTVGDPFKDTSGPSM
                     NILIKLMSIVSLVIAPTLAQIQQAKDDDVKVQKKVERSVVVTRQPLQKQIK"
     gene            4027..4224
                     /gene="rpmI"
                     /locus_tag="VGM24_10750"
     CDS             4027..4224
                     /gene="rpmI"
                     /locus_tag="VGM24_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY4155900.1"
                     /translation="MPKVKTNSSAKKRFKITGTGEITFQKAFKRHILTKKSKKRKRLM
                     KKKGSVSAANQDFVKRLLRLK"
     gene            4302..4646
                     /gene="rplT"
                     /locus_tag="VGM24_10755"
     CDS             4302..4646
                     /gene="rplT"
                     /locus_tag="VGM24_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY4155901.1"
                     /translation="MPRSVNAVASRARRKRILKQAKGFYGKRKNVYTVAKNVLEKGLT
                     YRYVGRKLKKREYRALWIARINAAVREEGLTYSRFIHLLNEKGITLDRKVLADIAMNE
                     PEAFKQLISSVK"
BASE COUNT         1295 a         1224 c         1176 g         1172 t
ORIGIN      
        1 cggttccccg ttagcaaaag gtaaatcagc cacagaatcc ccatctgcca gatgctgttt
       61 agcaaagacc agcccagcac ggccaggctg ccggaattct ggagttgaag catgctattg
      121 ctttttcagg ttattgagta gtagctgtat ctcttccagc tcttcggggg aagccttgtg
      181 atttcccagg gcatgcaaca ccagttcggt ggaggatccg ccaaacaggg tgctgatcat
      241 tttgccgatc aaatgtttct gggttttctc ccggcttacc accggatgat aaatatgggt
      301 ctttaccgaa tcatcccgac ccaccagccc cttttcattc ataatctgca tgagcttcag
      361 ggtcgtggtg tagcccgctt ccttggtctt ggaaagctcc tcgtgaactt cgcgtacgct
      421 ggcggtattt ctttcccata atatccgcag gatctccaac tcgctttcgg ttggtttgat
      481 atattttgta gttcccatgg cgtcctattt aatatggaaa atacgatatt attcgtaatc
      541 aagcggtcat tttaacacaa aacttacatt tgcaggcagg gattcatgcc cgttattcca
      601 attacaaaaa tttatatatg gccaaccagc gcgattcagg atattatgca gggaaataca
      661 agatcccgga aggaaagccc gtttctttaa aagagcttcc gacggatgac aaggataagc
      721 aggtttcaaa ggaggaaggg gaggacctgc tgaaatccga tatcctgaag ttgacggaac
      781 tccaggagag attatacagc agtaaccgct acagcgtact gatggtgatc caggccatgg
      841 atgccgccgg aaaggacagc gtcgtgaagc atgttatgag cggactgaac ccgcagggag
      901 tagtggtcac ttcttttaaa acgcctacgc atcaggaatt ggagcacgat tatttatggc
      961 gccattatct ggccctgccg gcccggggcg ttgttggaat tttcaaccgc tcccactacg
     1021 aaaatgtact ggtcacgcgc gttcatccgg aatttatcct gaatgaaaat ctgcccggaa
     1081 ttacaaaagt ggaggatatc gatcattctt tctgggaaag aagattcaag caaatcaatc
     1141 attttgaaaa gaccctggca gataatggaa tggtgattct caaattccat ctgcatgtat
     1201 caaaaaaaga acagaaaaag cggttcatgg aaaggattga agatccctca aaaaactgga
     1261 aattttcttc ggcggatctg aaggagcgcg ccttgtggaa ggattaccgg aaggcgtatg
     1321 aggacatgct ggagaatacc tcaaaggata atgctccctg gttttcggta ccggcagatg
     1381 acaaatggtt cacccggctc gtgattgcca atattatcgt gcatgaactg agcagcctgg
     1441 atctgcacta tccggagttg agcagtgccc ggaaaacgga cctgcttaaa tcaaaagccg
     1501 atttgctgaa tgaagattta tgaacgccgg gcggggcgga ttttccgctc ccggcacctg
     1561 ttcattttat ttgtttttgg agaggctgtc gggtgacgac cacacttctt tccacttttt
     1621 tctgaacttt tacatcatcg tccttggcct gttgaatctg ggccagggta ggggcaatca
     1681 ccagcgaaac aatggacatc agtttgatca ggatgttcat ggaagggccg gaagtatcct
     1741 tgaatggatc gcccaccgta tctccggtaa cagacgcttt atgcggttct gattttttgt
     1801 aatggatctc cccgtttatt tcaactcctt tttcgaatga ttttttcgca ttgtcccagg
     1861 cgcccccggc attattctgg aacatgccca tcagcacgcc gctcacggtg gccccggcaa
     1921 ggaaaccgcc cagggcttcg ggtcccagca ggaatccgat gaccagcggg gaaaggatgg
     1981 caatggcgcc cggcgccatc attttcttaa tggacgcatc cgtggatatg gccacgcatt
     2041 tatcgtattc gggtttgccg gtgccttcca tgatcccggg gatggtcctg aattgcctgc
     2101 gcacttcttc caccatggcc atggcagcct ggcccaccgc acggatggcc agggaagaaa
     2161 aaacaaaggg gatcatgccg cccacaaaaa ggctggccaa tacttttgct ttataaatat
     2221 ctatcccgga tatgccggct acaccaacaa aagccgcaaa cagggcaagg gaggtcaatg
     2281 ctgcggaagc aatggcaaac ccttttccgg tagcggcggt ggtatttccc acggcatcga
     2341 gtatatccgt tttttctctc acttcttttg gcagttcact catttccgcg atacctcccg
     2401 cattatcggc aataggcccg aatgcatcaa tggccagctg catggcggtt gtagccatca
     2461 tgccggctgc ggcaatggcc actccgtaaa gtccggcaca ggcaaaagaa ccccagatgc
     2521 ctccggcgag aacaaggatg ggcagacagg tcgactccat accaatggcc agcccgccga
     2581 tcacattggt ggcgtgcccg gtagaagact ggcggatgat ggaaagcacc ggccttctgc
     2641 cgatggccgt ataatattct gtgatcaggc tcatcagggt tccaacaagc agtcccacac
     2701 cgatggctcc cagcacgccc catttgctga attcaattcc gcggagttcc atggtttccg
     2761 gaagaacgta aaagaccagg ccgaccgacg caattgccgt caggatgatc gaaccccagt
     2821 tgcccatatt aagcgcccgt tgcaccgtgc cggtacggat gctggccgtt tcactgattt
     2881 ttacgaaaag ggtcgccaca atggaaaaga gaatgcctac tccggcaatg agcatgggca
     2941 gcaggatcgg agacattccg ccaaaagcgt cgtcggcctt tgtttcctgt ccgagcacca
     3001 tggtggccaa aacggtagcc acataagacc cgaaaagatc ggcacccatg ccggccacgt
     3061 ctcccacgtt atcaccgaca ttatcggcaa tagtagcggg gtttctgggg tcatcttccg
     3121 gaatgccggc ttccactttt cccacgagat cagcgcctac atcggctgct ttggtataga
     3181 tcccgccgcc tacccgggca aaaagggcta ttgattccgc accaagggaa aacccggtca
     3241 gcacctcaat ggtcaggcgc atttcttccg aattgacacc tgcatccggg gcaaaaacgg
     3301 cttttaagat gatgaacagt ccgccaagcc ctaaaacggc aagacccgca acgcccagtc
     3361 ccatcacgga tcctcccgta aaggagacct tcagggccct gctcaggctg gtccgggctg
     3421 cgtgtgcagt ccgtacattc gctttggtcg ccacgcgcat gccgatatat ccagcggtgg
     3481 cgcttaatat ggcgcccagt ataaaggaga tggaaatagc ccagtgggag tggggattgg
     3541 tgatggacat aaagcccagc aaaagggcca caaccaccac aaaatagccc aggatcttcc
     3601 attctgcttt gagaaaagcc attgcccctt ccgcgatatg ccggctgatc tctttcatac
     3661 gttcagtgcc ttcttcctgc ctggaaaccc atttgaattt cacaaaagtg tacagcaagc
     3721 caatgattcc catgagcggt accagataaa tcaatttgtc catgataagt agatctgaga
     3781 ttttaaggga ggcaaaaata gtggaaaaca gttaaaacca gtctaattgt tccggttata
     3841 ttgagggata ttcccataaa gatcaggata ttattcccgt ttttcgaaaa aatgcataac
     3901 ttcgcgctcc atttttaatg gcaggtaaat cccggcaacg ggataacctg attgcccaaa
     3961 tggcccccca agtgatttgc ccgtgtaaat ccgccagcag ggtatcatat aaaacctcat
     4021 cacaagatgc caaaggttaa gacaaattcg agtgccaaaa aaaggtttaa gattaccggc
     4081 actggtgaaa tcactttcca gaaagcgttc aaacggcata tcctaaccaa aaaatcaaag
     4141 aagcgaaaac gcctgatgaa gaagaaagga agcgtgagcg cggccaatca ggattttgta
     4201 aaacgcctgc ttcgtttgaa gtaaaccggg ccttcccgga agcttctgcg gaggaagcat
     4261 ttttaaatta ttccattcac taattttcag attattttta tatgccacgt tcagtaaatg
     4321 cggttgcctc cagagcccgc agaaaacgga tcctcaaaca ggcaaagggt ttctacggaa
     4381 agcgaaaaaa cgtttatacc gttgccaaaa atgtattgga aaagggcctt acctaccggt
     4441 atgtgggccg caaactgaaa aaaagagaat accgggcttt atggatagcc cgtatcaatg
     4501 cagcggttcg cgaagagggc ctgacctatt cccgttttat tcacctgctg aatgaaaaag
     4561 ggatcacgct cgaccggaaa gtactggccg atatcgccat gaacgagccg gaagccttca
     4621 aacagctgat ttcttctgta aaataaactc tttgtcattt ttcagaggtt tcttaaaagg
     4681 gagagctatc ttttaagaaa cctcttttat tttgagattg ttttcaaaaa attacatttg
     4741 tacctgaaat ttcttcactt ctaaaacgtc aagccatcat caacccgaac gaatccatga
     4801 acaatacgta cgccgacctg gtgaagcaaa ccttcaattt cccccaggaa ggcttcgacg
     4861 ttaagga
//
LOCUS       DASYCU010000533         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2147835, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000533 DASYCU010000000
VERSION     DASYCU010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2147835"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VGM24_10760"
     CDS             <1..868
                     /locus_tag="VGM24_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HEY4155902.1"
                     /translation="VADKALSLEGKDLDKNGALASIEGLSTELQKNGKFNEVKTLNPP
                     GLKTVGLDIFPASLSWDEVEQICRENKMDALFALEVFDTDSKLSYTANPVNMQVPIVG
                     NVPGIEHQANLQTVVKTGWRIYDPSGKNILDEYTMSRQLSFSGRGINPVAAAAAIMNR
                     NEAVKEVANQAGQAYAQRIIPFWIRVNRDYYVRGTDNFVVAKRKAQTGNWDEAAKLWE
                     KETSSNSKKVAGRACYNMAIISEINGDLDAAIKWAQRSYENYNNRLALHYVNILKNRQ
                     VNDSIVQNQNGE"
     gene            complement(880..1596)
                     /locus_tag="VGM24_10765"
     CDS             complement(880..1596)
                     /locus_tag="VGM24_10765"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4155903.1"
                     /translation="MDYSRKSGTEEIRKRFDQDVERFSNLETGQQTVIDSPMLLDLTT
                     GAAFFVNPQARNLLDIGCGAGNYTLKMLEKIPGLNCVLVDLSMPMLVRAEERVSKATP
                     GRVDILQADILKADLPEHHFDIVLAASVLHHLRGTEEWINVFNKIFNSLNPGGSFWIS
                     DLITQDTEPLNRLFSAQYARHLESLGGEAYRKKVLDYVEREDSPRSLNFQLDLMKQVG
                     FRHTEILHKNGSFAAFGGIK"
     gene            complement(1692..>2324)
                     /locus_tag="VGM24_10770"
     CDS             complement(1692..>2324)
                     /locus_tag="VGM24_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4155904.1"
                     /translation="QTFIQLLDKNFDDRDMVKKYLEKIGSSAQRMAKLIRGVLDYSRL
                     NDYEEKQLETDLNLILEQVKTDFELVIKEKGAVIISNPLPVVKGMPLQLNQLFSNFIS
                     NSLKFAVGRPEIRISSRLVSGREIMPLSGVQRPDPYYEIVFEDNGIGFDPKFGDQIFT
                     MFRRLHGHQDFPGTGIGLALCKKIAENHKGFIQAEGNPDKGASFRVYLPV"
BASE COUNT          633 a          578 c          547 g          566 t
ORIGIN      
        1 tgtggcagac aaagcgcttt ccctcgaagg caaagacctg gataagaatg gggcgctggc
       61 aagcattgaa ggactgagca ccgaattgca gaagaacgga aaattcaatg aagtaaaaac
      121 cttgaatccg cccgggctta aaaccgttgg cctggatata tttccggcat cgctgagctg
      181 ggacgaagtg gaacaaatat gccgggagaa taaaatggat gcgcttttcg cgctggaagt
      241 ttttgatacc gattccaaat tgagctatac tgccaatccg gtaaacatgc aggtacccat
      301 cgttggaaat gttcccggaa tagaacatca ggcaaacctg cagacggtag taaaaacggg
      361 atggagaata tatgatccct ccggtaaaaa tatcctggat gaatatacca tgtccaggca
      421 attatctttt tccggaagag gtattaatcc cgttgcggcg gccgcagcga tcatgaaccg
      481 caatgaagcc gttaaggaag tcgccaacca ggccggccag gcgtatgcgc agcggatcat
      541 tcctttctgg atccgggtaa accgcgatta ttatgtaagg ggaaccgata attttgtcgt
      601 tgccaaaagg aaggcacaga ccggaaactg ggacgaagcg gctaaattat gggaaaagga
      661 aaccagcagc aacagtaaaa aagtggccgg aagggcctgc tacaatatgg cgatcatcag
      721 cgagatcaac ggggatctgg atgcggcgat aaaatgggcg caaagatcct atgaaaatta
      781 taataaccgt ctcgccctcc actacgtcaa tattctgaaa aaccgccagg tgaatgattc
      841 gatcgtgcag aatcagaatg gcgaataacc ggcccgggtt tatttgatcc ctccaaaagc
      901 ggcgaagctg ccgttcttgt gcaaaatttc cgtatgccgg aagccaacct gcttcatcag
      961 atcaagttga aaattaaggg agcgcggact gtcttcccgt tcaacatagt ccagcacttt
     1021 tttccggtaa gcttccccac ccaatgattc cagatgccgg gcgtactgag cggaaaacag
     1081 ccggttcagg ggttcagtat cctgcgtgat gagatctgag atccagaaac tgccaccggg
     1141 gttcagcgaa ttaaaaattt tattaaacac atttatccat tcctccgttc cgcgcagatg
     1201 atgcagcacc gatgcggcca gaacaatatc aaaatgatgt tccggcaaat ccgctttcaa
     1261 aatatccgcc tgtaaaatgt ccacccggcc cggcgtagct ttcgacacgc gttcttcagc
     1321 acggacgagc atgggcatgc tcaggtcgac gagcacacaa ttcagaccgg gaattttttc
     1381 cagcattttc agcgtataat tccctgctcc gcacccgata tccaataaat tcctggcctg
     1441 cggattcaca aaaaaagcgg cgccggtggt cagatccagc agcatggggg aatcaatgac
     1501 ggtttgctgt cctgtttcca gattggaaaa ccgctcaaca tcctgatcaa accgcttccg
     1561 gatttcctcc gtaccggatt ttctggaata atccattttt caaattcgtt tattcgtttt
     1621 catgcctctt caaccgtttt cccagacaaa attaaggttg gcgggtgaaa actccgttta
     1681 gatcaatcct cttaaactgg taaataaacc cggaacgatg cgcccttatc cggattgcct
     1741 tcagcctgga taaaaccctt atgattttcg gcgatcttct tgcatagggc cagccctatg
     1801 ccggtgcccg gaaaatcctg atggccgtgc aggcggcgga acatcgtaaa gatctggtct
     1861 ccgaatttcg gatcaaaacc gatcccatta tcttcgaata cgatctcata gtaaggatcc
     1921 ggccgctgaa ctccggacaa tggcattatt tcccttcctg aaaccagccg ggaggaaatc
     1981 cgtatttccg gtctgccgac agcaaacttc agggaattgc ttatgaaatt ggaaaagagc
     2041 tgatttaatt gcagcggcat tccctttacg accggaagcg gattactgat aataacggcc
     2101 cctttttcct ttatcaccag ttcaaaatcc gtcttcacct gttcgaggat gaggtttagg
     2161 tccgtctcca gctgcttttc ttcataatca ttcaggcggc tgtaatccag cactcccctg
     2221 atcagtttcg ccatccgttg tgcggagctg ccgatcttct ccagatattt ttttaccata
     2281 tcccggtcgt caaaattctt atccagcagc tgaataaagg tttg
//
LOCUS       DASYCU010000534         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_839645, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000534 DASYCU010000000
VERSION     DASYCU010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839645"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1823)
                     /gene="dnaG"
                     /locus_tag="VGM24_10775"
     CDS             complement(<1..>1823)
                     /gene="dnaG"
                     /locus_tag="VGM24_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY4155905.1"
                     /translation="FKLNSTLKLFKLISPQTIQQILSRIDITEIIGGFVKLKKRGANY
                     LGLCPFHNEKTPSFTVSPAKEIYKCFGCGRSGNTISFLMEHEKYSYVEALKWLAGKYN
                     VEIEETESSPEVKIQQQTADSLYILNGFAQQFFSRILMQDEEGQNIGFSYLKERGFRD
                     DIIHKFQLGYSPGKADAFATEALEAQYNPEILQKSGLVVWRDGKAFDNYRGRIIFPIQ
                     NQTGKIAGFGARIIRNNDRAPKYINTPENEIYVKSKILYGSYFARQAIDKLDECLLVE
                     GYTDVVSLHQSGIENAVASGGTSLTPDQLRLIKKYTSNLTILYDGDAAGIKAAQRGMD
                     LAFEESLNVKLVLIPDQEDPDSFVRKRGAAAFRDLIAGSKKDFILFQLETSLAGVGDD
                     SSKKAQLVNQIAETISRINKAEDFTKQQDYIRRSSELLKIDENGLSSLVNKFIRERIS
                     KSENRNAISARQNPDEIISGPPEYDEELTDLLNKDEQQERAIIRSLLEFGLKSWDEEK
                     RVADYMLPEFEDEDMIDNKNLINILHIYKDWYEQGLEPTSRNFLYQEDQQLSALVVSI
                     MDFPYEVSENWKTFYEGKISTREELYKEEVRSTLTYLKLRK"
BASE COUNT          406 a          426 c          424 g          567 t
ORIGIN      
        1 attttccgca atttgagata ggttaatgtg gaacgcactt cctccttata gagttcctcc
       61 cgtgtggaaa tttttccttc gtagaaagtt ttccaattct cactcacttc atagggaaag
      121 tccatgatgg aaaccaccag ggcgcttaac tgctgatctt cctggtacag aaagttgcgg
      181 gaggtcggct ccaacccctg ttcgtaccag tccttataaa tatgcaggat gttgatcagg
      241 tttttgttgt cgatcatatc ttcatcctca aattcaggga gcatataatc ggctacccgc
      301 ttttcctcgt cccaggattt taatccgaat tccagcaggg agcggatgat ggcgcgttcc
      361 tgctgttcgt ctttattgag caggtcggtt agttcttcat cgtattccgg agggccggaa
      421 ataatttcat ctggattttg cctcgcggat attgcattcc tgttttcgga tttggagatt
      481 ctttcgcgga tgaatttatt caccagggaa ctgagtccgt tctcatcaat tttcagcagc
      541 tcggaagagc gacggatata gtcctgctgt tttgtaaaat cttcagcctt gttgatgcgc
      601 gaaatggttt ccgcaatctg gttcaccagc tgcgctttct tgctgctgtc atccccgaca
      661 cctgccagcg aggtttccaa ttgaaaaaga ataaaatctt ttttgcttcc ggcgatcagg
      721 tcgcgaaaag cggcggctcc ccttttgcgg acaaaactat cagggtcttc ctggtcaggg
      781 atcaaaacca gctttacgtt caggctttct tcaaaagcca ggtccatacc ccgttgtgcc
      841 gcttttattc cggcggcatc cccatcatat aaaatggtca ggttggaggt gtatttttta
      901 atcagcctga gctggtccgg ggtaagtgat gtgccgccac tggccaccgc attttctatc
      961 cctgactgat ggagggatac gacatcggtg tatccttcca caagcaggca ttcatccagt
     1021 ttgtcaatgg cctgccgggc aaaataggag ccatacagga ttttactttt tacatatatt
     1081 tcattttccg gcgtattaat gtatttcggc gcccggtcgt tgttgcgaat gatgcgtgct
     1141 ccgaaaccgg caattttccc ggtttggttt tgaatgggaa aaataatcct tccccggtaa
     1201 ttgtcaaatg cttttccatc gcgccacacg accagtccgc ttttttgaag aatctccgga
     1261 ttgtattggg cttccagggc ctctgtggca aatgcatcgg ccttgcccgg gctgtagccg
     1321 agttggaatt tatgaatgat atcgtcccgg aatccccgtt ctttcaggta agagaaacca
     1381 atattttgtc cttcttcatc ctgcatcagt atcctgctga agaattgttg tgcgaacccg
     1441 ttgaggatat acaaactgtc tgctgtctgt tgctggatct ttacctcggg actgctttct
     1501 gtttcttcga tctccacatt gtattttccg gcaagccatt tcaatgcttc cacataagaa
     1561 tatttctcat gctccatcag aaaactgatg gtattgccgc ttcgtccgca tccgaagcat
     1621 ttatagattt ccttggcggg tgaaaccgtg aaggaaggag tcttttcatt gtgaaaagga
     1681 caaagcccga ggtagttggc gcctcttttt ttaagcttga caaaaccgcc gatgatttcc
     1741 gtgatgtcaa tgcggctgag aatctgttgt atggtttgcg gagagatcag tttgaagagt
     1801 ttgagcgtcg aatttaattt aaa
//
LOCUS       DASYCU010000535         6642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1424375, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000535 DASYCU010000000
VERSION     DASYCU010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424375"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /gene="rplV"
                     /locus_tag="VGM24_10780"
     CDS             <1..391
                     /gene="rplV"
                     /locus_tag="VGM24_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY4155906.1"
                     /translation="SYHENKPEKMEALAKLKNYPTSPRKMRLLADEIRGLQVEKALAL
                     LEHHPQHSSTPLYKLLWSAVKNWEQKNEGKSAADAALLVKTVFVDGARVLKRMRPAPQ
                     GRGYRVRKRSNHVTLIVDEKTEKEESK"
     gene            491..1276
                     /gene="rpsC"
                     /locus_tag="VGM24_10785"
     CDS             491..1276
                     /gene="rpsC"
                     /locus_tag="VGM24_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY4155907.1"
                     /translation="MGQKTNPIGARLGIIRGWESNWYGSKKDYAFKLIEDNKIRTYLN
                     ARINKGGIAKIVIERTLNKLIVTIHTSKPGIIIGKGGGEVDRIKEELKKLTGKEDVQI
                     NILEIRRPELDANIVADTIARQIENRINYKRAIKMAIASALRMGAEGIKVKVGGRLGG
                     SEIARSEEIKQGRTPLHTLRMDIDYANVFALTVYGKIGVKVWICKGEVLTKRDLNPNF
                     VGGKSDVSDRRDRRDDLRDERGRGGDRDRRGGGERRGGGGGRR"
     gene            1331..1753
                     /gene="rplP"
                     /locus_tag="VGM24_10790"
     CDS             1331..1753
                     /gene="rplP"
                     /locus_tag="VGM24_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY4155908.1"
                     /translation="MLQPKRSKHRKAQKGRIRGIAKRGSEISFGSYGLKALEAIWLTN
                     RQLEAARQALTRSMKREGNVWIRVFPDKPITKKPLEVRMGKGKGNPEYWAAVVEPGRM
                     LFEADGVPEAVAKEALLLAAQKLPIKTKFVVRHDVTAL"
     gene            1849..2052
                     /gene="rpmC"
                     /locus_tag="VGM24_10795"
     CDS             1849..2052
                     /gene="rpmC"
                     /locus_tag="VGM24_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY4155909.1"
                     /translation="MAKRNEFVNSIKEMSETDLTARIREDELRLKKLKFAHAISPLES
                     PVTIRLLRKDIARLKTALTKKKV"
     gene            2090..2347
                     /gene="rpsQ"
                     /locus_tag="VGM24_10800"
     CDS             2090..2347
                     /gene="rpsQ"
                     /locus_tag="VGM24_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY4155910.1"
                     /translation="MPDRNLRKTRTGVVSSNKMDKTVVVAVERKVQHPIYGKFLKKTT
                     KFHAHDEKNECSIGDVVRIMESRPLSKTKRWRLVEIVEKAK"
     gene            2372..2740
                     /gene="rplN"
                     /locus_tag="VGM24_10805"
     CDS             2372..2740
                     /gene="rplN"
                     /locus_tag="VGM24_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY4155911.1"
                     /translation="MIQQESRLNVADNSGAKEVLCIRVLGNSGQDYARIWDKIVVTVK
                     DALPAGGIKKGTVAKAVIVRTKNKLRRKDGSYIRFDDNAVVLLTTSDEPRGTRIFGPV
                     ARELRDKGYMKIISLAPEVL"
     gene            2808..3146
                     /gene="rplX"
                     /locus_tag="VGM24_10810"
     CDS             2808..3146
                     /gene="rplX"
                     /locus_tag="VGM24_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY4155912.1"
                     /translation="MNNRFKPKFNIKKGDKVVIISGDDKDLKKSRKVLEVFPDESRVL
                     VEGVNIVTKHSKPSAQNTKGGIVKMEAKVHISNVSLWDAKSGGPSKAKRTRENGKLIR
                     IAKKSGEVIK"
     gene            3146..3727
                     /gene="rplE"
                     /locus_tag="VGM24_10815"
     CDS             3146..3727
                     /gene="rplE"
                     /locus_tag="VGM24_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY4155913.1"
                     /translation="MSTAKYTARLADKYKKEVVPALMKKFGYKTVMQAPKLEKICLNR
                     GINGAVADKKLVDISIEELTTITGQKAVSTVSKKDISNFKLRKGLPIGARVTLRGEKM
                     YEFLDRLVAAALPRVRDFKGVSEKSFDGRGNYTMGITEQIIFPEVDIDKVNKITGMDI
                     TFVTTANTNEEAYELLKELGMPFKNAKKEGEPA"
     gene            3830..4099
                     /gene="rpsN"
                     /locus_tag="VGM24_10820"
     CDS             3830..4099
                     /gene="rpsN"
                     /locus_tag="VGM24_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEY4155914.1"
                     /translation="MAKESVKARQRKREALVTRYAEKRAALKAAGDFKALDLLPRNSS
                     SVRLKNRCQLTGRPRGYIRYFGVSRGIFRDMALQGKIPGVRKASW"
     gene            4144..4542
                     /gene="rpsH"
                     /locus_tag="VGM24_10825"
     CDS             4144..4542
                     /gene="rpsH"
                     /locus_tag="VGM24_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY4155915.1"
                     /translation="MVTDPIADFLTRVRNAQMANHRIVEIPASNLKKRMTEILYDKGY
                     ILKYKFEEDNKQGVIKIALKYDPQTKEPAIRSLERVSRPGLRQYASPLEFTRVKNGLG
                     IAIVSTSKGVMTDKEAKAQNVGGEVLCHVY"
     gene            4648..5202
                     /gene="rplF"
                     /locus_tag="VGM24_10830"
     CDS             4648..5202
                     /gene="rplF"
                     /locus_tag="VGM24_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY4155916.1"
                     /translation="MSRIGKKPVIIPKGITVSVSSDNIVTVKGPKGELKQSIDRDIKV
                     EVKNDQVEFSRPTDQIRHRSLHGLYRSLMSNMVKGVSDGYKRQLELIGVGFKASNQGN
                     LLDLALGFSHNIIFEVPKELKVATAQEKGENPKIILEGSDKQLIGQVAAKIRGLRKPE
                     PYKGKGVRYTGEVVRKKAGKAAGK"
     gene            5251..5601
                     /gene="rplR"
                     /locus_tag="VGM24_10835"
     CDS             5251..5601
                     /gene="rplR"
                     /locus_tag="VGM24_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY4155917.1"
                     /translation="METLLKRQKIRYRIRKKVSGSALKPRLSVFRSNNEIYVQLIDDL
                     EGKTLASASSRDKDIAAQKGTKTEKSRLVGTAIAGKAKELGIDRAVFDRGGNLYHGRV
                     KAVAEAAREAGLQL"
     gene            5635..6156
                     /gene="rpsE"
                     /locus_tag="VGM24_10840"
     CDS             5635..6156
                     /gene="rpsE"
                     /locus_tag="VGM24_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY4155918.1"
                     /translation="MSTTNVVSKIKAGDLELKEKVVSINRVVKTTKGGRAFSFSALVV
                     VGNEAGVVGHGLGKAKEVQEAITKGIEDAKKNLIRVPVMHGTIPHDQLAKEGAAKVLI
                     KPAAHGTGVIAGGSMRAVLESAGITDVLAKSLGSANPHNVVKATFKALTLLREPIQVS
                     RTRNLALKKVFNG"
     gene            6272..6451
                     /gene="rpmD"
                     /locus_tag="VGM24_10845"
     CDS             6272..6451
                     /gene="rpmD"
                     /locus_tag="VGM24_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY4155919.1"
                     /translation="MKKIRITQIKSGIDRSERQKLTLKALGLKKMNNSREVEATPQIL
                     GMVRAVDHLVKVEEI"
BASE COUNT         2076 a         1340 c         1867 g         1359 t
ORIGIN      
        1 atcatatcac gagaacaaac ctgaaaaaat ggaagcatta gcgaaactga aaaattaccc
       61 gacatcgccg cgcaagatgc ggttgctggc agatgaaata agggggcttc aggtggaaaa
      121 ggccctggcc ctgctggaac atcatccgca acattcttcc acgcccctgt acaagctgtt
      181 gtggtcggca gtgaagaact gggaacaaaa gaatgaaggg aagagcgcag ccgatgcggc
      241 cctgctggta aagacggtat ttgtggatgg cgcacgcgtg ctgaaaagaa tgcgtccggc
      301 cccgcaggga cggggttacc gggttcgtaa acgcagcaac catgttaccc tgatcgtaga
      361 tgaaaaaacg gaaaaggaag aatcaaaata aaaaattgac catttgaaaa agcatccgga
      421 tgggcccggt aaaagacata aagtcatttt caaatcatca aattatcaaa ttatcaaatc
      481 aatcagataa atgggtcaga aaacaaatcc tataggtgcg agactgggaa tcatccgtgg
      541 ctgggaatcc aactggtacg gaagtaagaa agattatgct ttcaaactga tcgaggataa
      601 taagatcaga acctatctca atgcccgtat caataagggc ggtattgcca agatcgtgat
      661 tgagcggacg ctgaacaagc tgatcgtgac cattcatact tccaaacccg gaattatcat
      721 tggtaaagga ggaggagagg tcgatcgcat caaggaggag ttgaagaagc tgaccggaaa
      781 ggaagatgtt cagatcaata ttctggagat ccgccgtccc gaactggatg cgaatattgt
      841 cgcggatacc attgcccggc agattgaaaa ccggattaac tacaaacggg ccatcaagat
      901 ggcgatcgct tcggcccttc gaatgggcgc ggaagggata aaggtgaaag tcggcggtcg
      961 tctgggtgga tcggaaatag caagatcgga agagatcaag cagggccgta cgccgttgca
     1021 taccctccgt atggacattg attatgccaa tgtatttgcg ctgaccgttt acgggaagat
     1081 cggggtgaaa gtctggatct gtaaagggga ggtcctgacc aagagagacc tgaatccgaa
     1141 ttttgtcggt ggaaagagtg atgtgagcga ccggagggac aggagagatg acctgagaga
     1201 tgagcgcgga cgcggagggg acagggaccg gagaggtggc ggagaaagaa gaggaggagg
     1261 gggcggccgg agataatacc tgcctggcca ttatcatatt taaattatca gattttcaaa
     1321 ttagaataac atgttacagc ccaaaaggtc caagcacaga aaggcgcaga aaggccggat
     1381 ccgcggaatt gcaaaaagag gcagcgagat ctctttcggt tcttatggat tgaaagccct
     1441 cgaagccatc tggcttacga accgccagct ggaagctgcg cgccaggcgc taacccgctc
     1501 catgaagcgg gaggggaatg tgtggatccg ggtgtttccg gataagccga taaccaagaa
     1561 gccgttggaa gtgagaatgg gtaaggggaa agggaatccc gaatactggg cggccgtagt
     1621 ggaacccggt cgtatgctgt ttgaagccga tggggtgccg gaagctgtgg caaaagaagc
     1681 tttattactg gcggcgcaga agctgcccat caagacaaag tttgtggttc gtcatgatgt
     1741 gaccgcacta taatttgaaa aaaggctttt gggaaatgaa attccggtcc gccggataaa
     1801 aaatgaattt tcaaattatc taattatcta attctcaaat caagcgagat ggcaaagaga
     1861 aacgaatttg taaacagtat aaaagagatg agcgaaacgg atctgacggc ccggatcagg
     1921 gaagatgagt tgcgtttgaa aaagctgaag tttgcccatg ccatatcgcc gctggaaagc
     1981 ccggttacca tccggttact caggaaagac attgcccggt taaagaccgc attgacaaaa
     2041 aagaaagttt gaagcattga cagggtatca aattatcaaa taagcaaata tgccagatag
     2101 aaatttacgg aaaacgagaa caggggttgt cagcagcaat aaaatggata aaaccgttgt
     2161 cgttgctgtg gaaagaaaag tacagcaccc gatctacggt aagttcctga agaaaacgac
     2221 gaaattccat gcccacgacg agaagaacga gtgcagtatc ggagatgtgg tccggattat
     2281 ggaaagccgt ccgttaagca agaccaagcg ctggcgcctg gtggagatcg tggagaaagc
     2341 gaaataattc gtaaatctta aatcgtaaga aatgattcag caggaaagca gacttaatgt
     2401 tgcagataac agcggtgcca aggaagtttt gtgcatccgg gtactgggaa acagcggaca
     2461 ggactacgcc aggatctggg ataagatcgt ggttacggta aaagatgctt tgcctgccgg
     2521 cggtataaaa aagggtacgg tagccaaggc cgtgatcgta agaaccaaaa acaaactgcg
     2581 ccgtaaggac ggttcttata tccgcttcga cgataatgcc gtggtattgc taacgaccag
     2641 tgatgagccc cggggcacgc gcattttcgg accggtggcc agggaactcc gggacaaggg
     2701 gtatatgaaa attatttccc tggctccgga agtactttga agaacttatt ttgaaataca
     2761 atttttaagc gcgtttaaga ttccgcttca agcgtaaaaa gagaaaaatg aacaacagat
     2821 ttaaaccgaa gttcaatatt aaaaaagggg acaaggtggt gattatttcc ggggacgaca
     2881 aggacctcaa aaaatcacgc aaggtgctgg aagtatttcc ggatgaatcc cgggtgctgg
     2941 tggaaggggt gaacatcgtc accaaacaca gcaaaccgtc tgcgcagaat accaagggag
     3001 gcattgtgaa gatggaagct aaagtgcata tatccaatgt atccctctgg gatgccaaga
     3061 gcggaggtcc ttccaaggca aaacgcacgc gggaaaacgg aaaactcatt cgcatagcaa
     3121 aaaaatcagg ggaggtaatt aaataatgag cacagcaaaa tacactgccc gcctggcaga
     3181 caaatacaaa aaagaagtgg tacctgcttt aatgaaaaag tttggttata aaaccgtcat
     3241 gcaggcaccc aagctggaaa agatctgttt gaaccgcggc atcaacgggg cggtggccga
     3301 caaaaaactc gtggatattt ccatagaaga actcaccacc attaccggcc agaaagcggt
     3361 ttcaacggtg tcaaaaaagg atatctccaa tttcaaactc cgtaagggtt tgccaatcgg
     3421 ggcccgggtt acgctgcgcg gagaaaaaat gtatgaattc ctggatcgcc tggtagctgc
     3481 ggccctgccc cgggtgcggg atttcaaggg cgtgagtgaa aaatctttcg atggccgggg
     3541 aaactatacc atggggatta ccgagcagat catttttccg gaagtggata ttgataaggt
     3601 gaacaagatc acgggtatgg atatcacgtt tgtcactacc gccaacacca atgaagaagc
     3661 atatgagttg ctgaaggaac tggggatgcc atttaagaat gcgaagaagg aaggcgaacc
     3721 ggcttaatcg gaaaaccggt ggctgcagaa tgcccgatgc ggacagggca gtggaagata
     3781 aaacgtctgc cggtttaaag gagtttataa taataaataa taaaagttca tggccaaaga
     3841 atcagtcaaa gccagacaaa gaaaaaggga agccctggtt acaagatacg cggaaaagcg
     3901 tgcagctctg aaagcggccg gtgattttaa agcgctggat ctccttccga ggaattcatc
     3961 ttccgtgcgt ttgaaaaacc gctgtcagct tacggggcgc ccgaggggat atatccggta
     4021 tttcggggtt tcccggggaa tattccggga catggcttta caggggaaaa ttccgggtgt
     4081 gcggaaagcc tcctggtaag gggttgaaac agggtaagat tttaataaat taatcgagat
     4141 acaatggtaa cagatccaat agcagatttt ctgacaagag tccgtaatgc gcagatggcg
     4201 aatcaccgga tcgtggagat cccggcgtcg aacctgaaga agcgtatgac agaaatattg
     4261 tacgataaag gctatattct gaaatacaaa ttcgaggaag acaacaaaca gggggtgatc
     4321 aagatcgcgc tcaaatacga tccgcaaacc aaggagccgg ctatccggag cctggaaagg
     4381 gtgagtcgcc cgggtttgcg ccagtatgcg agtcccctgg aattcacccg ggtgaaaaat
     4441 ggattgggta tagccatcgt atccacttca aaaggagtga tgaccgacaa ggaagccaag
     4501 gcccagaatg taggcggtga ggtattgtgt catgtttatt aatgtaatga ttgagaacag
     4561 ctggttattg taactggggg agcggtggtg aggaggaaat aatcagatcg gttcatttta
     4621 atattttcaa attttcaaat caacagcatg tccaggatag gaaaaaaacc ggtaatcatt
     4681 ccaaaaggga tcacggtgag cgtgagcagt gacaatattg ttacggttaa aggacccaag
     4741 ggggagctga agcagtccat agaccgggat attaaggtgg aggtaaagaa tgaccaggtg
     4801 gaattttccc gtcctacgga ccagatccgt caccgctccc tgcatggctt gtaccgttcc
     4861 ctgatgtcca atatggtaaa gggggtttct gacggctaca aaagacagct ggaattgatt
     4921 ggtgtgggtt ttaaggcatc caaccaggga aacctgctgg atctggcgct cggtttttcg
     4981 cataatatca ttttcgaagt gccaaaggaa ctgaaagtag ccacggcaca ggagaaggga
     5041 gagaacccga agattattct ggaaggaagc gacaaacagc ttatcggcca ggtggctgcc
     5101 aagatccgtg gtcttcgcaa gccggaaccc tacaagggta aaggagtgcg gtacaccggc
     5161 gaagtagttc gtaagaaagc cggtaaggct gcgggaaaat aatttgagat ttcaaattat
     5221 cagattttaa aattttcaaa ttacgcaagg atggaaacat tactaaagag acagaaaata
     5281 agataccgga tccggaagaa agtttccggc agcgccctca aaccccggtt gtccgttttc
     5341 agaagcaaca atgaaatcta tgttcagcta attgacgatc tggagggaaa aacgctggcc
     5401 tctgcttctt cccgcgacaa ggatattgcg gcacagaaag gaacgaaaac ggaaaagagc
     5461 aggctcgtgg gaactgccat tgccggcaag gccaaagaac tcggaattga tcgggccgtt
     5521 tttgaccggg gcggaaatct gtatcacggc cgggtgaagg cggtggcaga agcagcccgc
     5581 gaagcgggat tgcagttata agatgaaaaa aaagtttatt cgtaaatatt tatcatgtca
     5641 acaacgaatg tagtttcaaa gataaaggcc ggcgacctgg agctaaagga aaaagtagtt
     5701 tccatcaacc gggtggtgaa gaccaccaag gggggacgtg ctttcagctt ttctgctctg
     5761 gtggtggtgg gtaatgaagc gggtgtggtg ggtcatggtc tgggaaaggc caaagaagtg
     5821 caggaagcca ttacaaaggg aattgaggac gccaagaaaa accttatccg ggttcctgtc
     5881 atgcacggca ccattcccca cgatcagctc gccaaggaag gcgcagccaa ggtgctcatc
     5941 aagccggcag cacatggtac cggggtaatt gccggaggca gtatgcgggc ggtgctggag
     6001 agtgccggga tcaccgatgt gctggcgaaa tcgctgggtt cggccaatcc gcataatgtg
     6061 gtcaaagcaa ccttcaaggc cctcacctta ctgcgggaac cgatccaggt gtccagaaca
     6121 agaaacctgg ctttgaaaaa agtattcaac ggataagccg gcattgacta ggtgaaatcg
     6181 aatcggctgc gatatatttt caggataacg gcagacagtg aaaatttaac ctggcaggtt
     6241 ccatccggcg gaaaaatata aaattgagca aatgaaaaag atcaggatca cacagataaa
     6301 aagcggcatt gaccggtcgg aacgtcagaa actgaccctg aaagcgcttg gattaaaaaa
     6361 aatgaataac tccagggagg tcgaagccac tcctcagata ctgggtatgg tccgcgcggt
     6421 ggatcacctg gtgaaagtgg aggaaatata atttattacc cggctccggt tttcaaattt
     6481 tcaaattgag caaatgaact tacatacaat caggccggct aaaggcgcaa cacataagga
     6541 aaaaagactt ggaaggggag aagcttccgg taagggtgga acttcgacca aggggaacaa
     6601 aggcggacaa agccgggccg ggtaccagcg caaaatggcg ca
//
LOCUS       DASYCU010000536         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4896246, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000536 DASYCU010000000
VERSION     DASYCU010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4896246"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VGM24_10850"
     CDS             <1..926
                     /locus_tag="VGM24_10850"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY4155920.1"
                     /translation="KETVVSFEILPPLKGKTISSIYDHLDPLMEFKPAFINVTYHRSE
                     TMFKKKADGTFEKVEVRKRPGTVGICAAIMNHYRIDAVPHLICGGFGKRETEDALIDL
                     HFLGVDNVLVLRGDAARNEASFNPEPGGNRYAIDLLKQVVGLNHGIYLEEDILNGGKT
                     NFCIGVAGYPEKHFESPNLATDLMNLKAKVDAGAEYVTTQMFFDNKKYFEYVKACREI
                     GIEVPIVPGLKPITSKKQLSMLPKVFHVDIPADLSDAVMKSKTDEEVECIGTEWLLQQ
                     SRELKKAGVPVLHYYTLGKPKVIRNVVKELV"
     gene            940..1827
                     /locus_tag="VGM24_10855"
     CDS             940..1827
                     /locus_tag="VGM24_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY4155921.1"
                     /translation="MAFTDYYKILEVPKNASSEDIKKAYRKLARKYHPDLNPKDKEAN
                     KRFQLINEANEVLSDPEKRKKYDQYGEDWAHADQFEKARQSQQQAGSHRQPEDPFSGD
                     FGSGEYSDFFEQLFGGGSKSRSAKFRGSDYQAELHLPLRDAAKTHSQTLTVNNKNIRI
                     SIPAGVEDGQVIKLKGHGGPGANGGPAGDLYITFVIPPDPVFKRSGNDLYSTINLDLY
                     TAVLGGEILVETLDGKIKLKVNPETQNGTKTRLKGKGFPVYKQEGTFGDLYLTYQVLI
                     PQNLTQKQRELFTELSNLK"
     gene            1834..2124
                     /locus_tag="VGM24_10860"
     CDS             1834..2124
                     /locus_tag="VGM24_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEY4155922.1"
                     /translation="MSDPEIISLETCCSSYNIELEFIHSLEEYGLIRIRYRETKGFIE
                     EEDVRLLEQFSRMYYDLNINVPGIDALQHLLERIRQLQQESEALRAKLRIYE"
     gene            2157..>3227
                     /locus_tag="VGM24_10865"
     CDS             2157..>3227
                     /locus_tag="VGM24_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY4155923.1"
                     /translation="MKQQILFVALLGVCCCCFSQEPIALRYNKSSGMIPMRDGVKLHT
                     VILTPVDRSHPYPFLIMRTPYGADAHLPEESGIITLTSRFNYASMAADGYIFIFQDVR
                     GKFRSEGSMQIHQPIIHLKQPGAVDESTDTWDTIDWLLHHVDNNNGKAGITGISYPGW
                     LALVGAVDPHPALKASSEQACMGDLFLGDDFHHNGAFRLSYGMEYSYEVENDPARDSD
                     FPFPQFDLFDWYRDLGSLKNVNQKYFRGKIPTWNNFVKHPDYDSFWQKNSPLSYVTRP
                     EIPQLHVGGYYDQEDINGPQLMYGYMEKEDSFNRNHIVLGPWFHGQWGSQKGDSIGKI
                     YFGSNTGDWFRELQKKWFDYYLK"
BASE COUNT          905 a          760 c          832 g          730 t
ORIGIN      
        1 caaaagaaac cgtagtctcc tttgaaattc ttcctcccct gaaagggaag accatcagtt
       61 ccatttatga tcacctggat cccctgatgg aattcaagcc ggcatttatc aatgtcacct
      121 accaccgcag cgaaacgatg ttcaagaaaa aggccgatgg cacgtttgag aaagtggaag
      181 tgagaaaaag gccgggaacc gtaggaattt gtgcagccat catgaaccac taccgcatcg
      241 atgccgttcc gcacctgatc tgcgggggtt tcggaaaacg ggaaacggaa gatgcgctga
      301 tcgacctgca ttttctaggg gtggataatg tgctggtcct gcggggagat gcagcccgga
      361 acgaagcctc ctttaatccg gagcccggcg gcaaccggta tgcgatcgac ctgctgaaac
      421 aggtggttgg tctgaaccac ggaatatacc tggaagaaga tattctgaac ggcggcaaaa
      481 ccaatttctg tatcggggtg gcgggttatc cggagaagca ttttgaatcg cccaacctgg
      541 ctacggacct gatgaacctg aaagcaaaag tggatgccgg cgcggaatac gtgaccacgc
      601 agatgttttt tgataataag aaatactttg agtatgtgaa agcctgccgg gagatcggaa
      661 tcgaagtgcc catcgtgccg ggattgaagc ccatcaccag caaaaagcag ctgagcatgc
      721 tcccgaaagt atttcatgtg gacattcccg ccgatctttc cgatgcggtt atgaaaagta
      781 aaaccgatga agaagtggaa tgcattggca cggaatggct gcttcagcaa agccgggaac
      841 tgaaaaaagc cggcgtgccg gtcttgcatt attataccct gggcaaacca aaagtaatcc
      901 ggaatgttgt gaaagaactg gtttaattag aaattccata tggcttttac tgattattat
      961 aaaatcctgg aggtcccgaa aaatgcatct tccgaagata taaaaaaggc ttaccggaag
     1021 ctggcgagaa agtaccatcc cgatctcaat ccgaaagaca aagaggcaaa taaaagattc
     1081 cagctcatca atgaagcaaa cgaagtgctg agcgatccgg aaaaaagaaa aaaatacgac
     1141 cagtacgggg aagactgggc ccatgccgat cagtttgaaa aagcccgtca atcgcagcag
     1201 caggccggtt cccatcgtca gccggaagat cctttttccg gcgatttcgg cagcggtgaa
     1261 tactccgatt tttttgaaca gctctttggt ggcggttcca aatcccgttc tgcaaaattc
     1321 cggggatccg actaccaggc ggaactgcat ctcccgctgc gtgacgcggc aaaaacacat
     1381 tcccagacct taaccgtcaa caataaaaat atccgcatca gcattccggc cggcgtggaa
     1441 gacgggcagg ttatcaagct caagggccac gggggtccgg gcgccaacgg cgggcccgcg
     1501 ggtgacctct atattacctt tgtgatcccc ccggatccgg tttttaaaag atccgggaat
     1561 gatctgtaca gcacgataaa cctggatctt tataccgccg tactgggagg cgagatcctg
     1621 gtggaaacgc ttgacgggaa aataaagctg aaagtaaacc cggaaacaca gaacggaaca
     1681 aagacccgat tgaaaggcaa ggggttcccg gtatataaac aggagggaac ctttggagat
     1741 ctgtatttaa cgtaccaggt attgatcccg caaaacctta cgcagaaaca gcgggaactg
     1801 ttcaccgaac tttcgaacct gaaataaaac gaaatgtccg atccggaaat catatcgctc
     1861 gaaacctgct gttcttctta caatatcgaa ttggaattca ttcattcgct ggaagaatat
     1921 ggactgatcc gcatccgtta ccgggaaacg aaaggattta ttgaggagga ggacgttcgc
     1981 ctgctggaac agttcagcag aatgtattat gatctgaaca tcaatgtgcc cggtattgat
     2041 gcgcttcagc atttgctgga aaggatcagg caacttcagc aggagtccga agccctgcgc
     2101 gcgaagctga ggatttacga ataaacgatc cggctcattc caagatattt ctgcttatga
     2161 aacaacaaat actcttcgtt gccctgctgg gcgtttgctg ctgctgtttt tcccaggaac
     2221 ccattgcgct ccggtataac aagtcttccg ggatgatccc catgcgggac ggagtgaaac
     2281 tgcatacggt tattctcaca ccggtggacc ggtcacatcc ttatcccttt ctgatcatgc
     2341 gcacgcctta tggcgcagac gcgcacctgc cggaggaaag cgggatcatc acccttacca
     2401 gccggttcaa ttatgccagt atggctgccg atgggtacat atttattttt caggatgtgc
     2461 gcgggaaatt caggagtgaa ggctctatgc agatccatca gcccattatt catctgaaac
     2521 aaccgggagc tgttgacgaa agcacggata cctgggatac gattgactgg ttattgcatc
     2581 atgtggacaa taacaacggc aaagcaggga tcaccggaat atcctatccc ggctggctgg
     2641 cgctggtggg agccgtagac ccgcatccgg cactgaaggc ttcttctgaa caggcctgca
     2701 tgggagacct tttcctggga gatgattttc atcacaacgg cgccttccgc ctgagctatg
     2761 gcatggaata cagctatgaa gtggaaaacg atccggcgcg tgacagtgat ttcccttttc
     2821 ctcaatttga tttgttcgac tggtaccggg acctgggctc tttaaaaaat gtgaatcaaa
     2881 aatattttcg gggaaaaata cccacctgga acaactttgt aaagcatccc gactacgata
     2941 gcttctggca aaagaattcc ccgctcagtt atgtgacccg tcctgaaatt ccccaattgc
     3001 atgtaggtgg ttattatgac caggaggaca tcaacggacc gcagctgatg tatggttata
     3061 tggagaagga agacagtttc aaccggaatc atattgtgct gggaccctgg tttcacgggc
     3121 agtggggaag ccagaaagga gacagtatcg gtaaaatata ttttggcagc aataccggag
     3181 actggttcag ggaactgcag aagaaatggt ttgattatta tctcaag
//
LOCUS       DASYCU010000537         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3740486, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000537 DASYCU010000000
VERSION     DASYCU010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3740486"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="VGM24_10870"
     CDS             <1..1817
                     /locus_tag="VGM24_10870"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY4155924.1"
                     /translation="TDDTKAIQDAIDRYPNIYVPQGWYRVSGTLRLKPNTVLIGLSPI
                     ATQFILANNTEAFGSFGGPCPLLESSRGGKNILSGIGLSTNADNPRAVACKWMAGAGS
                     YMNDVKFIGGHGSMERIWKQPQHQPERNYRGDGTDPSWDTEYWSLWITDGGGGVFQDI
                     WTANTYASAGVYISNTSATGRIYALSVEHHVRNEVRFNRVANWKVYALQLEEESREGT
                     ECQPLELEDCHDMVFANLYMFRVIRIIKPYPYSIRNWGCSGVEFLNVHNYSQIKYTSA
                     VPLYDVNTGTEVRPWEFNRLYIAGREKGEKLSNCEENGVEKLATGFELAEGSCSDSRG
                     NIYFCDSRLRRIYKWSAATQTTTLLADYHWEPLSLACDRKDNLLVVFKYVPKPGFLRN
                     GKPEVYTNPPDAAGTSFSAWGNSGFGTLVYSIDPANPDETIRLLEKKKMESLPAIYKA
                     LYPAHRWRDSHDFNTITGSRADECFVAPDSVTIIPVVYDLARAACLAEAFPGKPLYIA
                     DEYDKRTVKLEVNARGEVSGLRYFAEKGEFSTATDPSGNVYIADGEIYVFDPAGKQIR
                     LIKTPERPTTLIFAGKNKQELYFTGEHSLYRIRSQPAE"
     gene            complement(1802..2500)
                     /locus_tag="VGM24_10875"
     CDS             complement(1802..2500)
                     /locus_tag="VGM24_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155925.1"
                     /translation="MEDSELKNIWHEYDRRMEEAKLLNLQSWALNMRWFEEMQIRKAQ
                     SSLHFLIRFKVRAVVLGILWVLFLSVLVYGNQLRNGFLTVSLGMIILFNLLATVVYIK
                     HIVLIRRIDYSEPVTDTQERLAALQLSTLWIVRILWLQMPFYITWFWRRDWIDFGSLR
                     FWLILFPITLFFILLAVFLYRNINIKNVDRKWVRKFLMAGPEYKSLVRAKKFVAEIEE
                     FKKELVLNTVHSAG"
     gene            complement(2503..3006)
                     /locus_tag="VGM24_10880"
     CDS             complement(2503..3006)
                     /locus_tag="VGM24_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4155926.1"
                     /translation="MSAQQAKENFLKLIQENKGIIIRICNSYCPDKHYREDLAQEIIF
                     HLWKSGDHYDPNQKFSTWMYRVALNVAISYYRKGKTSVYPLSLDGEQHEVEDQRADET
                     EENINLLQRFIHELGKMDKALMILFLEGKTYAEIADILGISETNVGTKISRIKKRIKQ
                     NFSDLNK"
     gene            3181..4137
                     /gene="rbsK"
                     /locus_tag="VGM24_10885"
     CDS             3181..4137
                     /gene="rbsK"
                     /locus_tag="VGM24_10885"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEY4155927.1"
                     /translation="MKPDILVVGSSNTDMVIRSERLPAPGETILGSQFYMNPGGKGAN
                     QAVAAARLGGRVSFIAKTGNDIFGEQSVKLFREEGINTDFLLSDPENPSGVALITVDR
                     NGENCIVVASGANGTLSKSDLLPAQSRIENSGLVLMQLEIPLETVCYVAAVAAARGIP
                     VILNPAPACDLTDEQLENISIITPNIREAEMLTGTRISDLESAKIAAQKIFSRGPDTV
                     IITLGERGVLVFHEKQFTHIPGFKVKAIDTTAAGDVFNGALAVALAEGIPMLESTRFA
                     GKAAAISVTRAGAQSSAPFKMELDKFEFILSEISYGSNNNFL"
     gene            4161..>4506
                     /locus_tag="VGM24_10890"
     CDS             4161..>4506
                     /locus_tag="VGM24_10890"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEY4155928.1"
                     /translation="MKKIFTTTLAWLLMLTGFGQQTFQISKEKLKDKIKGGWAGQTIG
                     VTFGGPYEFRFDGTFIQDYQPLLWYSGYVKNTMLNDPGLYDDLYMDLTFVNIFDKYGL
                     HAPVDSFANAFAH"
BASE COUNT         1125 a         1187 c         1141 g         1053 t
ORIGIN      
        1 ctaccgatga taccaaagcg atccaggacg ccattgaccg ctacccgaac atctatgttc
       61 cccagggctg gtaccgggtg agcgggaccc tccggctaaa acccaatacg gtgctgatcg
      121 ggctgagccc catcgccacc cagttcatcc tggccaataa tacggaagcc tttggaagtt
      181 tcggtggccc ctgccccctg ctggaatctt cccgcggcgg aaagaatatc ctgagcggga
      241 tcgggctttc gacgaatgcg gacaatcccc gggccgtcgc ctgcaagtgg atggccggcg
      301 ccggttccta tatgaacgac gtaaaattca tcgggggtca cggcagcatg gagcgcatct
      361 ggaagcagcc gcagcaccag ccggaaagaa attaccgggg ggatggcacc gatccttcct
      421 gggatacgga gtactggagc ctttggatca cggatggcgg cgggggcgtt ttccaggata
      481 tctggaccgc caatacctat gcttcggccg gcgtctatat ttccaatacc tcggctaccg
      541 gccgcatata tgcgctttcc gtagagcatc atgtccgcaa cgaggtccgc ttcaaccggg
      601 ttgctaactg gaaagtatat gcgctccagc tggaagagga aagccgcgaa ggaacggaat
      661 gccagccgct cgagctggag gactgtcatg atatggtgtt tgcgaacctt tatatgttcc
      721 gcgtgatccg gatcatcaag ccctatccct attccatccg gaactggggt tgctccgggg
      781 ttgaatttct gaacgtgcac aactacagcc agataaaata taccagcgcg gttcccttat
      841 acgatgtcaa taccgggacg gaagtgcgtc cctgggaatt taaccggttg tacatcgccg
      901 gtagggagaa gggagaaaaa ttaagcaact gcgaagagaa cggggtggaa aaactggcca
      961 ccggatttga actggcggaa ggcagttgca gcgacagcag gggaaatatc tatttctgcg
     1021 attcgcggct caggcggatt tacaaatggt cggccgcaac gcagaccact accctgctcg
     1081 ccgattatca ctgggaaccc ttatccctgg cctgcgaccg gaaggacaac ctgctggttg
     1141 tttttaaata cgttcccaaa cccggttttc tgcgcaacgg aaaacccgaa gtatatacca
     1201 atccgcccga tgccgccgga acctctttca gcgcctgggg aaattccggg ttcggcaccc
     1261 tggtctattc cattgatccg gcgaatccgg acgaaacgat ccggttgctg gaaaagaaga
     1321 aaatggaaag tctgccggcc atttacaaag ccctctatcc ggcacaccgc tggcgggatt
     1381 cgcacgactt taatacgatc accggcagca gggccgatga atgtttcgta gcccccgaca
     1441 gtgttaccat catcccggtg gtttacgatc tggcgcgggc cgcctgcctg gcggaagcct
     1501 ttccgggaaa gcctttgtat atagcagatg aatacgacaa gcggacggtg aagctggagg
     1561 tgaatgcccg gggcgaagtg tcgggactcc gttattttgc tgaaaaagga gaattctcta
     1621 cggcaacgga cccgtccgga aatgtataca tcgccgacgg ggagatctat gtttttgatc
     1681 ccgccggcaa acagatccgg ctcatcaaaa cgcccgaacg gccgaccacc ctgatctttg
     1741 ccgggaaaaa taagcaggaa ctttatttta cgggggagca ttccctgtac cggatccgtt
     1801 ctcaaccggc agagtgaacc gtattcaata ccagctcctt tttaaattct tctatttccg
     1861 ccacaaattt ctttgcccgg accagggatt tgtattccgg tccggccatc aggaatttcc
     1921 tgacccattt tctgtccacg ttcttaatgt taatattccg gtacagaaaa acagccagca
     1981 ggataaaaaa caacgtgatg ggaaagagga tcagccagaa tcttaaactg ccaaagtcaa
     2041 tccagtccct ccgccagaac caggtaatat aaaaaggcat ctgcagccag aggatgcgca
     2101 cgatccacag cgtggacaat tgcaaggccg ccagtctctc ctgcgtatcc gtaaccggct
     2161 cactgtaatc gatccggcgg atgagaacga tatgttttat atagaccaca gtagccagga
     2221 ggttaaacag aatgatcatc cccagggaaa cggttaaaaa tccgtttctc aactggttgc
     2281 catataccag aacagaaaga aaaaggaccc agagtattcc cagcacaacg gcccgtactt
     2341 taaaccggat caggaaatgg aggctggact gcgccttccg gatctgcatc tcctcgaacc
     2401 accgcatatt caatgcccag gattgcaggt tcagcaattt cgcttcttcc atcctccggt
     2461 catattcatg ccagatattc ttcaattcac tgtcttccat gatcatttgt ttaaatctga
     2521 aaaattttgt ttgattctct ttttgatccg gctgatcttc gttcccacat tggtttccga
     2581 aatacccagg atatcggcga tctccgcgta ggttttcccc tccaggaaca ggatcatcag
     2641 ggctttgtcc attttaccga gctcatggat gaaccgctgc aggaggttga tgttctcttc
     2701 cgtttcatcg gccctttgat cttccacctc atgctgttcc ccatccagcg aaagcggata
     2761 tacggatgtt tttcctttcc ggtaataaga gatcgcgaca ttcagggcca cccggtacat
     2821 ccaggttgaa aatttctggt tgggatcgta gtgatcgccg gacttccaca aatgaaagat
     2881 gatctcctgg gccagatcct cccggtaatg cttatccgga caataggaat tgcaaatccg
     2941 gatgatgatc cccttattct cctggataag tttcagaaaa ttttcctttg cctgttgtgc
     3001 actcattaac cgtgtaatat tccttttata atgattcgca gaagcccacg gaaaatcacc
     3061 gttttcccgg aatttatttt ataatatccc cccggaacgg ccgggaaaaa gaaaaggctc
     3121 ccgcatttcc ccggatatcc aattttaaga ttatcttttt atattgaaac gaagccggtt
     3181 atgaaaccag atattcttgt ggtgggaagc tccaatacgg atatggttat ccgctcggaa
     3241 cgcctgcctg ccccggggga aaccattttg ggcagccaat tctatatgaa tcccggggga
     3301 aagggagcaa accaggccgt ggcggcggcc cggctgggtg gccgggtgag ctttatcgca
     3361 aaaaccggga atgatatttt tggggagcag tccgttaaac tgttcaggga agaaggcatc
     3421 aatacggatt ttctgctttc ggatccggaa aatccttccg gggtggccct catcaccgtt
     3481 gaccggaatg gcgaaaattg tattgtagtc gcttccggtg cgaatggcac gctcagtaag
     3541 agcgatctgc tgccggccca atcacggatc gaaaattccg ggcttgtcct gatgcagctg
     3601 gaaatacccc tggaaacagt ctgctatgta gccgccgtag cggcagcccg gggcatcccg
     3661 gtaatattga accccgctcc ggcctgtgat ttaacggatg aacaattaga aaatatttcg
     3721 atcatcactc caaatatccg ggaagccgaa atgctgaccg gcacgcggat cagcgacctg
     3781 gaatcagcga agatcgcggc ccagaaaata ttcagccggg gtccggatac cgtgatcatc
     3841 accctggggg aacgcggtgt actggtattt catgaaaagc agttcacgca tatccccggt
     3901 tttaaggtaa aagcgattga tacaacggcc gccggcgatg tgttcaatgg tgcgctggcc
     3961 gttgccctgg cggagggcat tccgatgctg gaatccacgc gatttgcggg caaggcagca
     4021 gcgatttcgg tgacccgggc aggcgcccaa agttctgccc cgttcaaaat ggaactggac
     4081 aaatttgaat ttatcctttc ggaaatcagt tacggatcga ataataattt cctataaccg
     4141 gcaccggttc aaacacgctt atgaaaaaaa tctttacaac aaccctggcc tggctcctca
     4201 tgctgaccgg ctttggccag caaaccttcc agatcagcaa agaaaaacta aaggataaaa
     4261 ttaaaggcgg ctgggccgga caaacgatcg gggtcacctt cggcggcccg tacgaattcc
     4321 gcttcgacgg cacattcatc caggattatc agcctctttt atggtattcc ggttacgtaa
     4381 aaaataccat gttgaatgat ccgggcttat acgacgacct gtatatggac ctgaccttcg
     4441 tgaacatttt tgataaatac ggactgcatg ctcccgtgga ttccttcgcc aacgcttttg
     4501 cccatg
//
LOCUS       DASYCU010000538         3252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_551977, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000538 DASYCU010000000
VERSION     DASYCU010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551977"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VGM24_10895"
     CDS             complement(<1..850)
                     /locus_tag="VGM24_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY4155929.1"
                     /translation="MEQAPVHSHSDDHSHNHSGHSDGHHGHSHSHVVELKSVNTAFII
                     GIALNLAFVVVEVIAGLLINSLSLLSDAGHNLADVASLGLALFAFRMLKVKSNSTYTY
                     GYKKTTVLVALLNGGILLVSIGAILFESAHRLIRPEPLPGRTISIVAAAGILINGLTA
                     LLFFRTKENDLNIKGAFLHLMADAVISVALVIGGIVIYFTSWYWIDPLLGIAVAIAIV
                     FSTWKLLRDSLRLSLDGVPQGIDLRTVKETIEKIGGVRDFHHVHIWALSTMENALTGH
                     VVLNSGI"
     gene            1005..2258
                     /locus_tag="VGM24_10900"
     CDS             1005..2258
                     /locus_tag="VGM24_10900"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEY4155930.1"
                     /translation="MSLNLIDSIKSLLSGDPLGKAAGMLGEDPGNVQRAVDGIIPSIL
                     TGVLHRAGSGDVHGVMNLAVDAAKNGIPENLGALVGSGEGFNPLASAILNGLFGDKVS
                     GLTAAISSFSRISNKSASALLNVTAPASMAVLGKQVIDSNLNSAGLLSFLNSERDNIL
                     NALPSGLNLAGVLGFGSLSGIAGKLSGAASAGKADDRVAGGGSHATVRPGSGSRWLLP
                     LLLMLVLIAIIWYFMNRHSPESDLPVMSDSVSVVNDSASLPQSAGPEQVRISLSDGTV
                     LNVLKGSMEDQLVNFLNDSSSKPGKEVWFDFDSLDFKSGSAGISDDHNDQVQDMAAIL
                     KAYPKLKIKIGGYTDRAGDSLQNKKLSRDRAAAVAAAIKKAGAKPAQVTGSEGYGSAF
                     AKAAADAPDSVRKKDRHISVNVRSK"
     gene            complement(2264..>3252)
                     /locus_tag="VGM24_10905"
     CDS             complement(2264..>3252)
                     /locus_tag="VGM24_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEY4155931.1"
                     /translation="RTYFNRNFHIEPTNVCVFSCAFCSYSRLYSHREEGWELSVEQMM
                     DIVRKYDGKPVTEVHIVGGVHPKMNLPFFVELIQQIRAYRPELHIKGFTAVELDYMFR
                     KAKLSVEEGMKTLHDAGLDSLPGGGAEIFAAEIRKQICEDKVDADGWIRIHRAAHQLG
                     MHTNATMLYGHIEQSHHRIDHMDRLRTLQDETGGFNCFIPLKFRNKQNDMSQVAESTV
                     TGDMKLYAVSRLYMDNFPHLKAYWPMLGRQSAQLSLSFGVNDLDGTIDDSTKIYSMAG
                     SEEQHPSLSTTQLVALIKQVGREPVERDTLYHEIKNYKDMDLAAMSADSAYN"
BASE COUNT          781 a          810 c          880 g          781 t
ORIGIN      
        1 cgatcccgct gtttagaaca acatggccgg tcagcgcatt ttccatggtg ctgagtgccc
       61 agatatggac atggtgaaaa tcccgcaccc cgccgatctt ttcaatggtc tcttttaccg
      121 tgcgcagatc aattccctgc ggaacgccgt cgagcgaaag acggagactg tcccggagta
      181 atttccaggt gctgaataca atggcgatgg cgaccgcaat ccccagcaag gggtcgatcc
      241 aataccagct ggtaaaataa attacgatgc cgccgatcac cagcgcgacc gatatcaccg
      301 catccgccat caggtgcaga aaagcgcctt ttatattgag gtcgttttct tttgtccgga
      361 aaaaaagaag ggcggtaagg ccgttgatca gaataccggc ggcagctacg atggaaatcg
      421 ttctgcccgg aagcggttcc ggcctgatca gccggtgcgc ggattcaaag agaatagcgc
      481 cgatggaaac cagcaggatg ccgccattga gcagagccac gaggaccgtt gtttttttat
      541 atccgtaggt atatgtcgaa ttggatttta ctttcagcat gcggaaggca aaaagggcga
      601 gacccaggct ggccacgtcg gccagattat gaccggcgtc cgaaagcagg gaaagggagt
      661 tgatcagcag cccggcaata acttccacca ccacaaaagc caggttgagc gcaatgccga
      721 tgataaaagc ggtatttacc gatttcagtt caaccacatg gctgtgcgaa tggccgtgat
      781 gaccgtctga atggccggaa tgattatgtg aatgatcatc ggaatgggaa tgaacagggg
      841 cttgctccat atgccaaagt taaaaaatta gcgcttcgaa ggataatatt agaatttgac
      901 ggggcaggca atttattctt accttgagca acccgtccaa aagacatata tctgcgccca
      961 tacattcttc cggggatcaa tttttttatt taaaatcata gggcatgtct ttaaacctga
     1021 tcgattcaat taagagcctt ctttccggcg acccgctggg aaaagccgcc ggcatgctgg
     1081 gggaggatcc gggaaatgtt caacgggcgg tggatgggat aatcccttcc atcctgaccg
     1141 gtgtcttgca ccgggcaggg tcaggagacg tccacggcgt gatgaacctg gcggtcgatg
     1201 cggcaaagaa cggaatccct gaaaacctgg gtgcactcgt tgggagcgga gaggggttca
     1261 atcccctggc ttccgctatt ctgaacggtc tttttggcga caaggtcagc gggttgaccg
     1321 ctgcgatctc cagcttttcc cgtatcagca ataaatcggc ctctgcttta ttgaatgtaa
     1381 ctgctccggc ctcgatggcg gtgttaggta aacaggtgat agattccaat ctgaacagcg
     1441 ccggtctgct ttcgtttctg aatagcgagc gggataatat cctcaatgca ttgccctccg
     1501 gactcaatct tgcgggcgtg ttgggtttcg gaagtttatc cggtattgcc ggaaaattat
     1561 ccggtgcggc atccgccgga aaagcggatg accgtgttgc cggtggcggc agccacgcaa
     1621 cggtaagacc tggctccgga tcccgctggc tgcttcccct gttgctgatg ctggtgctga
     1681 tcgccattat ctggtatttc atgaaccggc actctccgga atctgattta ccggttatgt
     1741 cggattctgt ctcggttgtc aacgacagcg cctcgcttcc ccaaagcgcc ggtccggaac
     1801 aagtcaggat cagcctgtcg gatggaaccg tgttgaatgt ccttaaagga agcatggagg
     1861 accagttggt caatttcctg aacgattcca gcagcaagcc cgggaaagag gtctggtttg
     1921 attttgacag cctggatttt aaatccggca gtgccggtat cagcgatgac cataatgacc
     1981 aggttcagga tatggccgcc attttgaagg cctatccgaa actgaagata aagatcgggg
     2041 gatatacgga ccgtgccggg gacagtctgc agaataagaa attgtcgcgg gaccgtgcag
     2101 cggctgttgc cgcagccata aaaaaagcag gagccaaacc ggcccaggtg accgggtccg
     2161 aaggatatgg atccgccttt gccaaagcag ctgccgacgc gccggattca gtaagaaaaa
     2221 aagaccggca tatctctgtt aatgtgagat ctaaataagt tccttaattg tatgccgaat
     2281 ctgccgacat ggccgccagg tccatgtcct tgtaattttt gatttcatgg tatagcgtat
     2341 cgcgttcaac gggttcacgc cccacctgtt ttatcaaggc aaccaattgg gtggtggaca
     2401 gggaaggatg ctgttcttcc gaaccggcca tggaatatat tttcgtggaa tcatcgatcg
     2461 ttccatccag atcattcacc ccgaaggata gggacaactg ggcgctttga cggcccagca
     2521 tcggccagta cgctttcagg tgcggaaagt tatccatgta taaacggctc acggcatata
     2581 gtttcatatc ccccgttacc gtgctttccg ccacctggct catatcattt tgtttattcc
     2641 ggaattttag cggaataaag cagttgaaac ccccggtttc atcctggagg gtcctgaggc
     2701 ggtccatatg atcgattcgg tgatgggatt gctcaatatg cccgtagagc atggtggcat
     2761 tggtatgcat gcccagctga tgggcggcgc ggtgaatccg gatccagccg tctgcatcca
     2821 ctttgtcttc gcagatctgt tttctgatct ccgcggcgaa aatttccgct ccgccgccgg
     2881 gcagggaatc cagtccggca tcatgaaggg tcttcattcc ttcttccaca ctcagtttgg
     2941 ctttgcggaa catataatcc agttccacgg cggtgaatcc cttgatatga agctccggcc
     3001 ggtaggcccg gatctgctga atcagttcaa caaaaaaagg aaggttcatt tttggatgaa
     3061 cgccgccgac gatatgcact tcggtgaccg gctttccatc atatttccgg acgatatcca
     3121 tcatctgctc cacgctgagt tcccagcctt cttcccggtg cgaatataaa cgggaatagg
     3181 agcagaaagc acaggagaat acacacacgt tggtgggctc aatatgaaaa ttgcggttga
     3241 aataggttct gt
//
LOCUS       DASYCU010000539         7854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1567365, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000539 DASYCU010000000
VERSION     DASYCU010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567365"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /gene="glpK"
                     /locus_tag="VGM24_10910"
     CDS             complement(<1..874)
                     /gene="glpK"
                     /locus_tag="VGM24_10910"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY4155932.1"
                     /translation="MKKYILALDQGTTSSRAILFDQQGRIQSVAQKEFTQIFPQPGWV
                     EHDPNEIWSSQASVAAEAVVKIGADGRNIVAIGITNQRETTIVWDRQTGEPVYNAIVW
                     QDRRTAPFCEDLKKQGLTGTIRSRTGLVIDAYFSGSKIRWILDNVPGARRKAEDGKLA
                     FGTVDSWLVWKFTQGRVHVTDVTNAGRTMLFNIREQQWDAELLRILGIPQSMLPEVKQ
                     SSEIYGETENSLFADKIPIAGIAGDQHAALFGQMCLDKAMVKNTYGTGCFMLMNIGDR
                     FIESKNNLLTTIAWK"
     gene            complement(930..2495)
                     /locus_tag="VGM24_10915"
     CDS             complement(930..2495)
                     /locus_tag="VGM24_10915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY4155933.1"
                     /translation="MNRNALLNQLADRDIHWDLIIAGGGGTGLGIAVDAASRGFKTIL
                     LEQSDFAKGTSSRSTKLVHGGVRYLAQGNIGLVREALRERGILQRNAPHLVKNESFII
                     PNYEWWGAAFYSIGLKFYDLLSGRLSFGRSKYISREEVMKRLPNIKPAGLRSGVLYHD
                     GQFDDSRLAVNLAQTALEQGAVLLNYLRVTGLQKEQNRVTGLTARDEETGISYALKGK
                     TVINATGVFVDELLCMDEPDCKPLVRPSQGIHLVLDESFLDSEDALMIPKTEDGRVLF
                     AVPWHHRIVVGTTDTPLNEHSLEPVALEEEIQFILRAAGKYFTHAPRREDVLSVYAGL
                     RPLAVPQKENAKTKEISRSHKLIVSASGMISITGGKWTTYRQMGEDTVDQAIRTGGLP
                     KRSCVTRTLPIHGHLAAVDRKDHLYVYGADLDSVRELFSENAEWKKPLHPRLEYTGAE
                     VIWAVRCEMARTVEDVLARRTRALFLDARAAIEMAPAVAALMAHELKKGSEWELEQIV
                     SFSKIAEGYLLLK"
     gene            complement(2508..3935)
                     /locus_tag="VGM24_10920"
     CDS             complement(2508..3935)
                     /locus_tag="VGM24_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY4155934.1"
                     /translation="MKKNLLIFLLLPFLSCSLFSQPVAGVGPLPSERQLKWQDANFYL
                     FVHFGPNTFTDLEWGKGSEKEEVFNPTALDCRQWCRIAKAAGASGIILTAKHHDGFCL
                     WPSAYSKHTVAQSSWRNGKGDVLKELSEACKDAGLSFGVYLSPWDRNHPAYGTPAYND
                     VFVNMMKEVVQRYGPFFEFWWDGANGEGPNGRKQQYDWHRFESTMRSVAPNTVLFSDI
                     GPDCRWVGNERGFAGTTNWNLLDTAGFTRGAGAPPTDTLNRGNVNGANWIPAECDVSI
                     RPGWFYHASEDSLVKTPETLFEIYLKSVGRGANLLLNVPADRRGLISSFDSAALMGFA
                     RLRAESFHSSKLKKDQASVRLDGKDLMNELTDGKETTWISLGKNYRKEWLDIRFNKPQ
                     QLNCIQLKEPVQNGQRIKSFTIDLFNGNQELTRLSQTTVGHQRILTFPSLSVTEIRIK
                     ITDAKSDPLISEIGVYQIDEKLLEK"
     gene            complement(3983..5206)
                     /locus_tag="VGM24_10925"
     CDS             complement(3983..5206)
                     /locus_tag="VGM24_10925"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEY4155935.1"
                     /translation="MRRPKLLFVSLLLSGSVIGQTAPPAKPVYEFDNGSVPGWSSFEN
                     PTGEKGKAAMENGGAKGHPSDDLDPGETRVLLKLEGTGMINRIWVTVDDRSPEMLRSL
                     KLEMFWDQEKKPAVSVPFGDFFGMGLGRTAVFHNAFFADPEGRSFLCFIPMPFRSGAR
                     IQITNESGKRLGHIFYDIDFQKLAAWNPANLYFHAFWHRDTATRMGTDFEILPAVKGK
                     GRFLGVNAGIQENPVYKGCWFGEGEVKMWLDGDHAFPSLAGTGSEDYIGTGWGQGVFF
                     NDYSGCLLANQDEQGWAFYRFHIPDPIYFKSDCRVTLQQIGGSSKKQVAELQQKGVPL
                     IPVGLDNGKGPEIPLYKKDRVTNLRDPSLPDGFVNFYRSDDVSATAYFYLNAPADALP
                     ALQPVAVRVYKRPVP"
     gene            5282..6088
                     /locus_tag="VGM24_10930"
     CDS             5282..6088
                     /locus_tag="VGM24_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4155936.1"
                     /translation="MSFFNNKVVIVTGGSDGIGRALIEALIRAGARVATCGRHYDKLY
                     ALQLEYSQVMLHAQVCDVSHEEECKRFIKSTVDTFGTIDILINNAGISMRSLFDEVDT
                     EVIRRLMDVNFMGAVYCTKYALPYILKQRGTIVGISSISGYRGLPGRSGYSASKFALQ
                     GWLESLRTELMDSGVHVMWISPGFTSSNIRNAALNGQGQGQGESPLDESRLMSADTCA
                     RYILGAIEKRKRVLVLTFTGKLTVFMNKFFPSLADKLVHRYYFKNGQLTR"
     gene            6115..6924
                     /locus_tag="VGM24_10935"
     CDS             6115..6924
                     /locus_tag="VGM24_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY4155937.1"
                     /translation="MAIITLTSDFGLQDYIAGAVKGQLLQIDPGFHILDISHRVAPFN
                     YPQAAYICRNAIRQFPANTYHMILVNLFEQKPEQLLLACHNGQFIFCADNGLINMILP
                     PKPEQVIGIPLDPQASKNTLYCSQVIGKAILALEQGAAPETLGDPATGYIEKNELRPI
                     LNDNYMEGQIIFIDNFENVVVNITREEFEEQRRGRGFRIVFKRNENIDRISETYADVP
                     LGEKQALFNSANYLEVAINKGNAAGLFGLQDFTDQSLQHYLFYQTVRIYFD"
     gene            7033..>7854
                     /locus_tag="VGM24_10940"
     CDS             7033..>7854
                     /locus_tag="VGM24_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEY4155938.1"
                     /translation="MNFKKVNNISGWVVCLFACMVYVLTMEASGSFWDCGEFVSSAYK
                     LQIPHPPGAPLFVMMGRFFIILFGDNPMTAARAVNFMSAILSGLTILFLFWSITHFAR
                     KIVQKTNLPLTTSQIFTIISAGAVGALAYTFCDSFWYSAVEGEVYGSSSFFTALVFWA
                     ILKWEHVSEEPGADRWLIFIFYMMGLSIGVHLLNLLTIPAIVMVYYYKRYKPTAWGAV
                     FAFIVGCVITGLVQKFVIQYTIKGAAWFDIRFVNDLNLPFFSGFAFFFILLAAILV"
BASE COUNT         1759 a         2189 c         1920 g         1986 t
ORIGIN      
        1 tcttccaggc aatggtggtc agcagattat tttttgattc gatgaaccgg tccccgatat
       61 tcatcagcat gaaacaaccg gtgccatagg tattttttac catggcttta tccaggcaca
      121 tctgcccgaa taaggcggca tgctggtcgc cggcaatccc ggcgatggga attttatcgg
      181 cgaacaggct gttttccgtt tctccgtata tttcactgga ctgcttcact tcgggcagca
      241 tgctttgcgg aataccgagt atgcgaagca gctcggcatc ccattgctgc tcccggatat
      301 tgaacagcat cgtccggcct gcattcgtca catccgtgac atgtacccgg ccctgggtga
      361 atttccatac cagccaggaa tccacggtcc caaaggcgag ctttccatct tcggcttttc
      421 ttctggctcc cggtacatta tccagtatcc accggatctt ggatcccgag aaataggcat
      481 cgatcaccaa tccggtcctg gagcggatgg ttccggtgag tccctgtttt tttaagtctt
      541 cgcaaaacgg cgccgtacgc cggtcctgcc acacgatcgc attgtaaacc ggctcccccg
      601 tctgccggtc ccacacaata gtggtctccc gctgattggt aatcccgatg gccacaatat
      661 tccgtccgtc cgccccgatc ttcacgaccg cttcagcggc tacggatgcc tgtgaggacc
      721 agatctcatt gggatcgtgt tccacccatc cgggttgcgg aaagatctgc gtgaattctt
      781 tttgtgcaac ggattggatc ctgccctgct gatcaaagag gatggcgcgt gaactggtcg
      841 ttccctggtc taatgcaaga atatactttt tcataaagac aaattattca ctttatagta
      901 aaaaggcgct ccctttttat ttttgaattt tatttcaaca gcaaatatcc ttccgcaatt
      961 ttgctgaaag atactatttg ctccaattcc cattcgctgc ctttctttaa ttcatgcgcc
     1021 atgagggcgg caaccgcagg ggccatttcg atggctgccc gcgcatccag aaataatgcg
     1081 cgggttcttc gggccagtac gtcttccacg gtccgggcca tttcgcatcg tacggcccag
     1141 atcacttcgg ctcccgtata ttccagccgg gggtgcaatg gttttttcca ttctgcattt
     1201 tcgctgaaga gttcccgcac cgaatcgagg tcggcgccat aaacatacaa atgatctttt
     1261 ctgtccacgg cagccaggtg gccgtgaatg ggcagcgtcc tggtaacgca gcttcgtttc
     1321 ggaagtccgc cggttcgtat cgcctggtcc acggtatctt cccccatctg ccggtaagtg
     1381 gtccatttac ctccggtgat gctgatcatt cccgatgcag agacgatcaa tttatgacta
     1441 cgtgaaattt cttttgtctt tgcattttcc ttttgcggca cggccagcgg ccgcaatccg
     1501 gcatatacgc tgagcacgtc ttcccgccgg ggcgcgtgcg tgaaatattt tccggccgcc
     1561 cgcagaataa attgaatctc ttcttccagc gccacgggct ccaggctgtg ttcattcagc
     1621 ggcgtgtcgg tggtgcccac tacgatccgg tggtgccagg gcacggcaaa caatacccgc
     1681 ccgtcttccg tttttggaat catcagggcg tcttccgaat ccagaaagga ttcatccagc
     1741 acgaggtgaa ttccctggct gggcctcacc aggggcttgc aatccggctc atccatgcac
     1801 agcagttcgt ctacaaaaac acccgtcgca ttgatcaccg tttttccctt cagggcatag
     1861 gagattccgg tttcctcatc ccttgccgtt aaacccgtca cccggttttg ttctttctgc
     1921 aaaccggtca cccgtaaata attcagcagg acggccccct gctccagggc cgtttgcgcc
     1981 aggttcaccg ccagccggga atcatcaaac tgcccgtcgt gataaagcac tccgctacgc
     2041 agaccggcgg gtttaatatt cggaagcctt ttcatcactt cctcccggga aatatatttt
     2101 gaccggccga aactcagcct gccggaaagc agatcgtaaa attttaatcc gatggaataa
     2161 aaggctgcgc cccaccattc atagttggga ataataaagg attcgttctt taccaggtgg
     2221 ggtgcattgc gttgcagaat gccacgttcc cgcagggctt cgcgcaccag gccgatattt
     2281 ccctgcgcga ggtaacgtac gccgccatga accagtttgg tgctccggct ggacgtgccc
     2341 ttggcaaaat ccgattgttc cagcaagatc gttttgaatc ctctggaagc ggcatccacg
     2401 gcaattccca accccgttcc gccaccgccg gcaatgatca ggtcccagtg gatatcccgg
     2461 tcagccagct gatttaacaa cgcgtttctg ttcattggtc tgattactta tttctccagc
     2521 agtttttcgt caatttgata aaccccgatc tcgctgatca gcggatcgga tttggcatcc
     2581 gttattttta tccggatctc cgtgacggat aaagaaggga aggtcaatat ccgctggtga
     2641 ccaaccgtgg tttgcgagag gcgggtaagc tcctggttcc cattaaacag atcgatcgta
     2701 aatgatttta tccgctgacc gttttgaacg ggttctttca gctgaatgca attcagttgc
     2761 tgcggtttat tgaaccggat atccagccat tccttccggt aattttttcc caggctgatc
     2821 caggtagtct cttttccgtc cgtcaattcg ttcatcaggt ctttcccgtc gagccggacc
     2881 gaagcctgat cttttttcag tttggaagaa tgaaaacttt ccgcccgcag ccgggcgaat
     2941 cccatcaggg cggcggaatc aaatgaagag atcaggcctc ttcggtctgc cggcacattc
     3001 agcaataaat tcgcgccgcg gccaacggat ttcagataaa tttcgaatag agtttccggc
     3061 gtttttacca gggagtcttc cgacgcatga taaaaccatc ccggtcgtat gctgacatcg
     3121 cattccgccg gaatccagtt ggccccgttc acatttcccc ggttcagggt atcggtgggc
     3181 ggagcgcccg cgccccgggt gaagccggcc gtatccagca ggttccaatt ggtggtaccc
     3241 gcaaatccgc gttcattgcc cacccagcgg caatcgggac cgatatcgct aaacaggacc
     3301 gtgttgggcg ccacgctgcg catggtggat tcaaaacggt gccagtcgta ctgctgtttc
     3361 ctgccattgg gtccttcccc attggcgccg tcccaccaga attcaaagaa gggaccgtag
     3421 cgctgcacga cctctttcat catgtttaca aatacatcgt tgtatgccgg tgttccgtaa
     3481 gccgggtgat tccggtccca gggagaaaga taaacgccaa aagaaagccc ggcgtcctta
     3541 cacgcttcgg ataattcttt cagcacatct cccttcccgt tcctccaact gctctgcgcc
     3601 accgtatgtt ttgaataggc gctcggccac aaacaaaatc cgtcgtggtg tttggcggta
     3661 agaataatgc cgctggcgcc tgcggctttg gcgatccggc accactggcg gcaatccagg
     3721 gccgtggggt tgaacacttc ttctttttcc gagcccttgc cccattccag gtcggtgaat
     3781 gtattcggac cgaaatgaac gaataaataa aaattggcat cctgccattt cagctgtctt
     3841 tcactaggca aagggccgac gcccgccacg ggctgggaga ataaggagca acttaaaaat
     3901 ggcagaagca aaaagatcag caggtttttt ttcatctaaa aaaaatttaa ttcaggcata
     3961 cggatcccag gtccgcgatg cttcatggaa ccggtctctt gtacacacga acagcaaccg
     4021 gctgtaaagc cggaagagcg tccgccgggg cattcaggta aaaataagcc gttgccgaca
     4081 catcgtcaga gcggtaaaaa ttcacgaagc catccggcag cgaaggatcg cgcaggttcg
     4141 tcacccggtc ttttttataa agggggatct ccggtccctt cccgttatcc aggcccaccg
     4201 ggatcagggg cacgcccttt tgctggagtt cggcaacctg tttctttgaa ctgccgccga
     4261 tctgttgcag ggtcacccgg caatcgcttt tgaaataaat gggatccgga atatggaagc
     4321 ggtaaaaagc ccagccctgc tcatcctggt tggccagcag gcatccggaa taatcattga
     4381 agaatacgcc ctgtccccaa cccgttccga tataatcttc cgagccggta ccggcaagcg
     4441 aagggaaagc atggtctccg tccagccaca tctttacttc tccttcacca aaccagcatc
     4501 ctttgtaaac cgggttctcc tggatgcctg cattcacccc gagaaatctt cccttccctt
     4561 ttactgccgg gaggatttca aaatcggtgc ccatccgggt ggcggtatcg cggtgccaga
     4621 aggcatgaaa atacaggttg gcgggattcc aggcagccag cttctgaaaa tcgatatcat
     4681 aaaagatatg acccagcctt ttcccgcttt cgttggtgat ctgtatgcgg gcgccggacc
     4741 ggaatggcat cggaataaaa cacaaaaaag atcttccttc ggggtccgca aaaaatgcat
     4801 tgtgaaaaac ggcggtcctg cccaggccca ttccaaaaaa atccccgaag ggaacagaga
     4861 cggccggctt cttttcctgg tcccagaaca tttcgagttt cagggaacgc agcatttcag
     4921 gcgaacggtc atccacggtc acccatatgc ggttgatcat tcccgtacct tccagtttaa
     4981 gcaatacccg ggtctcgccc ggatccaggt catccgaagg atgccctttg gctcccccat
     5041 tctccatggc tgcttttcct ttttctcccg tcgggttttc aaagctgctc cagccaggca
     5101 cactgccgtt gtcaaactca taaacaggct ttgccggagg agccgtctga ccgataacag
     5161 aaccggataa caagagtgat acaaaaagaa gttttggtct tctcatagcc tgccgtattt
     5221 ggtgaattta tgaaattgaa tgagaaaatc ttaacctact tttgcgcata caattcttcc
     5281 catgtcattt ttcaacaata aagtggtcat tgtaacaggt ggttcggacg ggatcgggcg
     5341 ggcgctcatt gaggctctga tccgcgccgg ggcgagggtg gccacctgcg ggaggcatta
     5401 tgacaaatta tatgcgctgc agctggaata ctcgcaggtc atgttgcatg cccaggtctg
     5461 cgacgtgagc catgaggagg aatgtaaacg cttcattaaa tcaaccgtcg acaccttcgg
     5521 cacgatcgat atcctgatca acaatgccgg catcagcatg cggtcgctgt tcgacgaggt
     5581 ggatacggag gtgatccgcc ggctgatgga tgtgaatttc atgggcgccg tttattgcac
     5641 caaatatgcc cttccctata tcctgaaaca aaggggaacc atcgtgggga tctcctctat
     5701 ctccggttac cgcggtctgc ccggccggag cgggtattcc gcatccaaat ttgcgctgca
     5761 gggctggctg gaatcccttc gcacagagct catggattcc ggcgtacatg tgatgtggat
     5821 ttcaccgggc tttaccagct ccaatatccg gaatgccgcc ctgaacggtc agggtcaggg
     5881 ccagggagaa agtccgctgg atgaatcccg gctcatgagc gccgatacct gcgccaggta
     5941 tattctgggc gctattgaaa aaagaaaaag agtgctggtg cttaccttca ccggaaagct
     6001 cacggtcttc atgaataaat ttttcccctc cctggcagat aaactggttc accgctacta
     6061 tttcaagaat ggccagctga ccagatagac tatttaaaac cgaaccgatt actgatggcg
     6121 atcattaccc ttacttctga cttcggcctg caggactata tcgcaggggc ggtcaagggt
     6181 cagcttttac aaatagatcc cggtttccat atactcgata tctcgcaccg ggtggctccc
     6241 ttcaactatc cccaggctgc ttatatctgc cgcaatgcca tccggcagtt cccggcaaat
     6301 acctaccata tgatcctggt caaccttttt gaacaaaaac cggaacagct tctcctggcc
     6361 tgtcataacg gccagtttat tttttgcgca gacaacggac tgatcaatat gatcctcccg
     6421 ccaaagccgg agcaggtgat cggcattccc ctggatccgc aggccagcaa gaacaccctc
     6481 tactgcagcc aggtcatcgg caaagccatc ctggcactgg aacagggcgc agcacccgaa
     6541 accctcggtg atccggcgac gggctatata gaaaaaaatg aactgaggcc cattctgaat
     6601 gataattaca tggaggggca gatcattttt atcgacaatt tcgaaaacgt ggtcgtgaac
     6661 attacccggg aagaattcga ggaacagcgc cgggggcgcg gattccggat cgttttcaaa
     6721 aggaacgaaa atatcgaccg gatcagtgaa acctacgccg acgtgccgct cggcgagaaa
     6781 caggcgctct tcaattcagc caattacctg gaagtggcca tcaataaagg caatgccgcc
     6841 ggtcttttcg gcttgcagga ttttacggac cagtcgctcc agcactacct gttttatcag
     6901 acggtgcgga tctacttcga ttagcgctcc gggcgtttgc ataaagccca aaagatatcg
     6961 ttaaagccca caagagtatc gggcatctac caacttcccc ctatatttgt caatcctaaa
     7021 aatcaacgga atatgaattt taagaaggtg aataacatct caggctgggt tgtttgtctc
     7081 tttgcctgta tggtgtatgt tttaaccatg gaagcatcgg gcagtttctg ggattgcggt
     7141 gaatttgtct ccagtgccta caagctccag atcccccacc cgcccggagc cccgcttttt
     7201 gttatgatgg gccgcttttt tatcatcctt tttggcgata atcccatgac agccgcccgg
     7261 gccgtgaatt tcatgagcgc tatcctgagc ggactcacca tcctcttcct attctggtcc
     7321 attactcatt ttgcgcgcaa aatcgttcaa aagaccaatc ttccccttac tacaagccag
     7381 atctttacca tcatcagcgc gggagccgtt ggtgcgctgg cctatacctt ctgcgattca
     7441 ttctggtaca gcgcggtgga aggcgaagtc tacggctcat cctctttctt taccgccctg
     7501 gtcttctggg ctattctgaa atgggaacat gtttccgagg aaccgggcgc cgaccgctgg
     7561 ctgatcttta ttttttacat gatgggcctc tccatcggcg ttcatctgct gaacctgctc
     7621 accattccgg ccatcgtgat ggtctattat tataagcgct acaaacccac ggcctggggt
     7681 gcggtcttcg cttttatcgt cggttgcgtg atcactggcc tggttcagaa attcgtcatc
     7741 cagtacacca ttaaaggcgc ggcctggttt gatattcgtt ttgtaaatga cctgaacctg
     7801 cccttcttca gcggttttgc ctttttcttt atcctgctgg ccgcgatcct ggta
//
LOCUS       DASYCU010000540         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4897847, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000540 DASYCU010000000
VERSION     DASYCU010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4897847"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VGM24_10945"
     CDS             complement(<1..1097)
                     /locus_tag="VGM24_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY4155939.1"
                     /translation="MAPTQAPLAERLRPHSLDQLVGQQHLTGKASILRTAIEQGKVPS
                     MILWGPPGTGKTTIANIIAHSLNVPFYTLSAISSGVKEVREVIEEAKQKEKAILFIDE
                     IHRFNKGQQDALLGAVEKGIVTLIGATTENPSFEVNSALLSRCQVYVLKPLNDKDLLR
                     VMNMAIKKDPFFQGKQIELRETEALINISGGDARKLLNLLELVVESAGTEQIVVTDQL
                     VMDTAQQRIALYDKKGEQHYDIISAFIKSMRGSDPNAAVYWLARMIEGGEDVKFIARR
                     MLILASEDIGNANPNALLLANATFEAVNKIGYPESRIILSQCAVYLASSPKSNASYAA
                     IDEALAMVKRTGDLAVPLHIRNAPTRLMKNM"
     gene            1167..2552
                     /locus_tag="VGM24_10950"
     CDS             1167..2552
                     /locus_tag="VGM24_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY4155940.1"
                     /translation="MKIQLLLFRAFLSWAVLVFSFPAPAKAQKLSAVQNYGVNRPGVV
                     MVRTVFSADVYVNNMRLDSRNFNHLLDSLQKIDSSGAIYTPEQKLDIVLTDISNHPNR
                     FFKSSLDYIKQPEEITSSGTGFLITGDGYVATNCHLIDRDKTFIRRQFILSAFQQITE
                     ANISAFESAWATRFSDQQRSLLYNTYASVYARLFSMALYDLKKDIFIEYSADSTNGSS
                     VPVKKIAHVVIRGQPMPGKDIAILKIDGDSSLPTLQIAKDELPKVGEQLFVYGFPGPV
                     TNNNYVSSESAIEPTLTTGIVSALKKSVEGWPLIQMDANINRGSSGGPVCNERGEVVG
                     LTTFGSIENSGGLAAGLNFAIPVSILEEYLDSADVLAHPSDVSLIFGDGVIFYDRESY
                     KEALRKFQQVREINPKFPGLTSYIRDSEKRLRQNDERKASMIRYDLVIFGLLLGIALV
                     LFKFLKKRKLA"
     gene            complement(2561..>3052)
                     /locus_tag="VGM24_10955"
     CDS             complement(2561..>3052)
                     /locus_tag="VGM24_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEY4155941.1"
                     /translation="HHDRSKLFADEVTAIHDHLAAKNKTLWIWGDRLIDGRTTGIGEW
                     EGSYNDTWRAVDMIPKDVVICDWHYDRPDKTAVYFSMKGLRVLTCPWRNPGFAVQQIQ
                     DILNFRANSTPEMKDRFQGIMETTWSRTAMFMKGYYNKQPADENSAWNTYKIICEEIG
                     KLK"
BASE COUNT          716 a          773 c          764 g          799 t
ORIGIN      
        1 tccatattct tcatcagccg ggtcggcgca ttccggatgt gtaaagggac cgcaagatct
       61 cctgttcttt ttaccattgc cagcgcctca tcgatggccg cataagaggc attgctcttt
      121 ggagaagatg cgaggtaaac ggcgcattgc gaaagaatga tcctggattc gggatacccg
      181 atcttgttca ccgcttcaaa agtggcattg gccaggagaa gggcattggg attggcattc
      241 ccgatatctt cactggcgag gatcagcatc cggcgggcaa tgaatttgac atcttcaccg
      301 ccctcgatca tccgggcaag ccagtaaacc gcggcattgg gatcgctgcc ccgcatggat
      361 ttgataaagg cggaaataat gtcgtaatgc tgttctcctt ttttgtcata gagcgcaatc
      421 cgctgctgcg ccgtatccat caccagttga tcggtaacaa cgatctgttc cgtgccggcg
      481 gattcaacga ccagctccag caggttgagc agttttcgcg catccccgcc ggaaatattg
      541 atcagggctt ccgtttcccg gagttcaatc tgttttccct gaaaaaacgg atccttcttt
      601 atcgccatat tcattacccg gagcaggtct ttgtcgttca ggggtttcag tacatagacc
      661 tggcagcggc tcaacagggc gctattcact tcaaaggagg gattctcggt cgtggcgccg
      721 atcagggtca cgatgccctt ttcaaccgcg cccagcaggg catcctgctg ccctttgtta
      781 aaacgatgga tctcgtcaat gaacaggatt gctttttcct tttgttttgc ttcttctatc
      841 acttccctga cttctttcac cccggatgaa atggcgctta gggtatagaa cggaacgttc
      901 agcgaatggg cgataatatt ggcaatggtc gtctttcctg tgccgggagg cccccagagg
      961 atcatcgagg gcactttccc ctgttcgatg gccgttctga gtatactggc cttgccggtg
     1021 aggtgctgct gacccaccaa ttgatccagg gaatggggcc ggagcctttc ggcaaggggg
     1081 gcctgagtag gagccataga aaaaagttta cctttaatca acaacaagtt agccctttta
     1141 aggttttata taagggagat gaaaacatga aaatccaact tctattattt agggcttttt
     1201 taagctgggc cgtccttgtt ttttcctttc ctgctccggc aaaagcacag aagctttccg
     1261 cggtgcagaa ctatggcgtc aaccggcctg gggtggtcat ggttcgaacc gttttttcgg
     1321 cagatgtgta cgtgaataac atgcggctgg attcacgcaa tttcaaccac ctgctggact
     1381 ccctccagaa aattgatagc agcggggcca tttatacccc tgaacaaaaa ctggatatcg
     1441 tactaacgga tatcagcaat catcccaacc gtttttttaa atcatccctc gactacatta
     1501 aacaacccga agaaattact tccagcggca ccggctttct gatcaccgga gatggatatg
     1561 tggctacgaa ttgccacctg atcgaccggg ataagacgtt tatccgccgc cagtttatcc
     1621 tgtccgcgtt ccagcagatc acggaagcga atatctctgc ttttgaaagt gcctgggcca
     1681 cccggttttc agatcagcaa aggagcctgc tttataacac ctatgcctct gtttatgcac
     1741 gcctgttctc catggccctc tatgatctga aaaaagacat attcattgag tacagtgcgg
     1801 attcgaccaa tggatcttcg gttccggtaa aaaaaattgc gcatgtggtg atccggggcc
     1861 agcccatgcc cggaaaagat attgccattc tgaaaattga tggcgacagt tccctgccaa
     1921 ccctgcaaat agcgaaggat gaattgccca aagtgggaga acagttattt gtttacggtt
     1981 ttccggggcc ggtcacgaac aacaattatg tgtcctctga gtcggccatc gaacccacgc
     2041 tgacaaccgg tattgtaagt gcgctcaaaa aatcggtgga aggatggccg ctgatccaga
     2101 tggatgccaa tatcaaccgg ggcagcagcg gcggaccggt ttgtaatgaa agaggagagg
     2161 tggtcggact gaccacgttc gggagtattg aaaatagcgg tgggctggcg gccggattga
     2221 attttgccat tccggtttcc attttggagg aatacctgga cagtgccgat gtcctggccc
     2281 atcccagtga cgtatcgttg attttcggag acggggtcat tttctatgac cgggaatctt
     2341 acaaggaagc cctgcgcaaa tttcaacagg ttagggaaat caatccgaaa ttcccgggct
     2401 taaccagcta tatccgcgat tcggaaaaac gcctccgtca gaatgatgaa cgcaaagctt
     2461 ccatgatccg ctatgacctg gtgatattcg gcctcctgct gggtattgca ctggtattat
     2521 tcaagtttct caaaaaacga aagctcgcct gatccgtttt ttacttcagc ttcccgatct
     2581 cttcacaaat tatcttatag gtattccagg cagaattttc atccgccggt tgtttgttat
     2641 aatatccctt cataaacatg gcggtgcggg accaggtcgt ttccataatt ccctggaacc
     2701 ggtctttcat ttccggggtg gaattggcgc ggaagttcag tatatcctgg atctgctgaa
     2761 cggcgaaccc gggatttctc cagggacagg tcagtacccg taatcctttc atggaaaaat
     2821 ataccgcggt tttatccggc cggtcataat gccagtcgca gatcaccaca tctttcggaa
     2881 tcatgtccac agcccgccag gtatcgttat aactgccttc ccactcgccg atgccggtag
     2941 tgcggccgtc aatgagccgg tcgccccaga tccacagggt tttatttttt gcggcgaggt
     3001 gatcgtggat cgccgttact tcatccgcaa acaacttgga acggtcgtga tg
//
LOCUS       DASYCU010000541         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_552261, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000541 DASYCU010000000
VERSION     DASYCU010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552261"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGM24_10960"
     CDS             complement(<1..434)
                     /locus_tag="VGM24_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY4155942.1"
                     /translation="MILLRHTRFLKTVFLYSISAFGGPQGHLGMMMKTFVGRRKDVTE
                     EELMDYNAFCQLLPGASSTQVLTLIGYKRGGIPLAVLTLLIWITPACFIMGFFSFFIE
                     LMNQKGSHADLFKYIQPMTVGFLIYAAWKAFKVSVHNTITHC"
     gene            complement(580..1344)
                     /locus_tag="VGM24_10965"
     CDS             complement(580..1344)
                     /locus_tag="VGM24_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4155943.1"
                     /translation="MLVFLAFEASGQEVTIRGTVFNMYRTHPLEAVSVVSNSGRGTTT
                     DVNGNYSIRVNENDSLSFSYLGRATQFFAVKEMNPTTGFDIALHVNVTELSEVRVAPR
                     NYHLDSLQNRIDYEKSFDYKKPGFALTSPSSGLGVGLDLDALINMFKFKQIRRSLAFR
                     ERLIDDEEDKFVDHRFNRSIIKKITQLDGDQLDSFMTAYRPSYQFTKAANDYDFFDYI
                     KLAYSEFEFNLAHPGLIQRENIFKKEDSLRKEKPHK"
     gene            complement(1443..2057)
                     /locus_tag="VGM24_10970"
     CDS             complement(1443..2057)
                     /locus_tag="VGM24_10970"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY4155944.1"
                     /translation="MKETQIHIALFASGRSSNAEQIIHYFHQEAHPVSGRRVLVSLIV
                     CNKPGAGVLEIAERERIPSLLIQREEFFRGAHYLEELKKREISFLVLAGFLWKIPPEL
                     IRAYPDRIINIHPALLPAYGGKGMYGTAVHQAVISAGEKESGISIHQVDELYDHGQVL
                     FQVRCKVSADDSPDSLAKKIHALEHAHYPAAIARWMESKLSLNP"
     gene            2450..>2540
                     /locus_tag="VGM24_10975"
     CDS             2450..>2540
                     /locus_tag="VGM24_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4155945.1"
                     /translation="MAQDGKALFQSNCASCHNPIKQITGPALKG"
BASE COUNT          676 a          566 c          611 g          687 t
ORIGIN      
        1 atgcaatggg tgatggtatt gtgtaccgat accttgaacg ctttccatgc cgcataaatc
       61 aaaaaaccca cggtcatggg ctggatgtat ttaaaaaggt cggcgtgact gcctttctga
      121 ttcatcaatt caataaaaaa ggaaaaaaat cccatgatga agcaggccgg tgtgatccag
      181 atgagcaggg ttagtaccgc cagcggaatc ccgccccttt tataaccgat cagggtcagg
      241 acctgcgtgg aagaagctcc gggaagcagc tggcagaatg cattgtagtc catcagttct
      301 tcttcggtca catcttttcg cctgcccaca aaggttttca tcatcatgcc cagatggccc
      361 tgcggcccgc cgaaagcgga gatgctgtat aaaaaaacag tcttcaggaa cctggtatgt
      421 cggagaagaa tcatggatta ttttcagcag acagaaattg ccgccggacg ctacaattta
      481 gagattgtta gccaattgga aagaatagtt gaccgttgac ggatgagtgt tgaccgattt
      541 tatgggttcc ttccgacaat gttatacagc ttccacggcc tatttgtgcg gtttctcctt
      601 cctcaatgaa tcttcttttt taaatatatt ttccctttga atcagtccgg ggtgtgcgag
      661 attaaattca aattcgctgt aagcgagttt gatgtagtcg aagaaatcgt aatcgttggc
      721 agcttttgta aactggtaag aaggccggta agccgtcatg aaggagtcca gctggtcacc
      781 gtccagttgg gtgatcttct ttataatgga gcgattgaaa cggtggtcta caaatttatc
      841 ttcttcgtcg tcgatcagcc tttccctgaa agccagggaa cgccggatct gtttgaactt
      901 aaacatattg atcagcgcat ccaggtcgag gccgactccc agtccgctgg aagggctggt
      961 aagagcaaat ccgggctttt tatagtcgaa ggatttctcg tagtcgatcc ggttttggag
     1021 ggaatccaga tgataattcc ggggggccac ccgcacttcg ctcagttccg tgacattgac
     1081 gtgcagcgcg atgtcaaaac ccgtggtcgg attcatttct tttacggcaa aaaactgggt
     1141 ggcccggccg agataggaaa aactcaggga atcgttttca tttacacgga tggaatagtt
     1201 tccattgaca tcggtggtgg ttccccgtcc ggaattggaa accacactca ctgcttcgag
     1261 gggatgcgtc cggtacatgt taaaaacagt tccccgtata gtcacttcct gtccgctggc
     1321 ctcaaaagcc aaaaaaacaa gcaatcccgg caaaatccat tttctaaaat atgcgtaaaa
     1381 aacgggcaat aaggttgatt tttgatgatt tggaagcaaa ataacacgaa gcctccgaaa
     1441 tattatgggt ttaacgataa tttggattcc atccacctgg cgattgctgc cggataatgg
     1501 gcatgctcca gggcatggat ctttttggcc agactgtccg gactgtcgtc cgccgaaacc
     1561 ttgcagcgaa cctggaaaag aacctggccg tggtcgtata actcatccac ctggtgaata
     1621 ctgatcccgc tttccttctc ccccgcgctg atcaccgcct ggtgaacggc cgttccatac
     1681 atgccctttc caccataggc cggcagcagg gccgggtgaa tattaatgat gcggtcgggg
     1741 taagcgcgga tgagttccgg gggaatcttc cataaaaatc ctgccagcac cagaaaagag
     1801 atttctcttt ttttcaattc ttccaggtaa tgggcgccgc ggaaaaattc ttcccgttga
     1861 atcagtaatg aggggattct ttctctttct gcaatttcca gaacgccggc gcccggtttg
     1921 ttgcaaacaa tcagggagac caaaacgcgg cggccggaaa caggatgcgc ttcctgatga
     1981 aagtagtgga tgatctgttc agcgttgctg ctcctgcctg aagcaaagag ggcgatgtgg
     2041 atctgcgttt ctttcatgaa tcccgatctg ttttaagatt tatatctgcc tgagaaaaag
     2101 ttgacgacat gtgatatggc ctcaaccttt tcgtaaagat gggtgaaaaa tctcgtttcg
     2161 ttcatgccat tttagtaaag aaaattgaat gaaaatccga tgacaaatga atgacaattt
     2221 tttttgagaa aattatataa tcggctgaaa cgcaggccag atcagggaaa aaaaatttag
     2281 agcatgttgg tattttgtta atttcatggc ttacttttgc aaccgactga gaagattttc
     2341 aacatttata caacgaattg tatatatgca tcattacaga cctagtgcat tcagaatatc
     2401 ctatagcgtc ctcctcacca cagtaattct gctttttggt aatctatcga tggctcagga
     2461 tgggaaggct ttgtttcaaa gcaattgtgc gtcctgtcat aatcccatca aacaaataac
     2521 gggccctgca ttaaaggggg
//
LOCUS       DASYCU010000542         5576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3307214, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000542 DASYCU010000000
VERSION     DASYCU010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307214"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3022)
                     /locus_tag="VGM24_10980"
     CDS             complement(<1..3022)
                     /locus_tag="VGM24_10980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY4155946.1"
                     /translation="MALPHLIKYVYTNGTDEVIRRGKKIHAIGYVELIEYDELFETVT
                     FRIKDDSYSTYYKVYVQKFRDAKSLSVRCSCPYNLGDICRHEAAALIQLQELLDRNML
                     KAEEVHYDQSHTVVKMKAIDLKTIRLLCSAEVYEEAGNYLNGNKASIELAADETVKAN
                     VRIGGTNYPVLIRKNEERNFDTSSSYPDTTHPLCLPKVILFLQLLQSNGPYYFDTIRN
                     WDKEKNKLLEAYGYSLSDDLDGKFEFSYKEGKPFLRVLDGSIKRVSPAFPSRPKPAAE
                     KELVAEELEPEIRAGAAQRLGVVFNFNHAAYPGFLLEAVSGESNDQQTAYVGKVEKLE
                     LARFVNTEKFNEPDRQLVYAIRKTQEQEVNKYLNRNSPFSGMWENIIHAEGEELPDDT
                     KALIIEYLHPKFRKLFSELSENPFIFFLNGHKPFKTSNLESVNISPDVLTPYFRVSLQ
                     KNQVEVKCFTRITGQSIDLSLNKCKSNVLFFHNNNLYLWNKPEDIAVLAGFKEGRMFI
                     PKPEWPEQLRKTVLPLQKEYHVEFDPELVKEIKSGDPERKILLQEKGEYLLFQPIFTY
                     KGFETTASGKDELVVPDGDKVLLIQRNKEKESAFISGLQSLHSQFIQPQGSSGLALRG
                     TDVLKNNWFFLFMDAMQDMKVTVHGFDALKNFRFNTSRPQTKIHISSSTDWFDARVDI
                     LFGDQKVTIADVKKALANKQQFVPLHDGTLGVLPDEWVKRYALLFRVGEGKSNELRLS
                     RYHLSVIDELYENRNEEELIIHLEKKYEQLREFKTIREIDPPEHLVHTLRPYQVHGFH
                     WLNYLHEVNWGGILADDMGLGKTIQALSFLHHYKGHYGHLRALVVCPTTLIFNWENEI
                     KKFAPSLTYRIHHGGERLRNKEALNGFEVLITTYGTLRSDIKLLMDIPFDYVVLDESQ
                     AIKNPSSKVTKAACLLHAKHRLCMSGTPLQNNTFDIFAQMNFLNPGMLGSIEFFRQEF
                     AIPIDKFGEPDRKDHLRKLLYPFILRRTKEQVARD"
     gene            3433..5094
                     /locus_tag="VGM24_10985"
     CDS             3433..5094
                     /locus_tag="VGM24_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY4155947.1"
                     /translation="MTRSFFLFLLFLNASLPSAYSQRLRKADRQLMAQIQTDVTYLSN
                     DKLEGRRAGTPGEKLASDYIISSFEKSGLKPMGDSGTWRQTFEIYDGRDISHCVFTIN
                     NIPLILNSEYIPLAFSAARKVTGSPAVALQESGSPWFYDLKDLMDAAHNNPHVDMETA
                     LRAKAKALAGRGASAVIFYNSSNSKEEDGIEFNQAEGIVPEKIPVVFVTREGRKKYLK
                     DISQTLDVVIDIKMEEKKRWGHNIIGYLDKGAPYTVVIGAHYDHLGFGEDGNSLYRGK
                     EKMIHPGADDNASGTAALMELAGMLHKSKSLKSNYLFIAFSGEESGLLGSKYFTWHPV
                     VDLSKISYMLNMDMIGRLNDSSHTLIIGGYGTSPQWSSVISSSVNKKIFSLQLDSSGT
                     GPSDHTSFYLKDIPVLFFFTGIHPDYHRPTDAAARINYPGEVQVIKLIYSITEKMDGM
                     PKPAFSKTRDTQYGETPAFNVTLGIMPDYSFTGNGVRIDAISEGRPAEKAGLQTGDVI
                     IRLGQYPVNSLQTYMQALSRFNREDKTTVEISRGTEVKTMDIQFN"
     gene            5106..5540
                     /locus_tag="VGM24_10990"
     CDS             5106..5540
                     /locus_tag="VGM24_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HEY4155948.1"
                     /translation="MKLKLKNGDLEDDFFEGSRLLGIVAPLKGYRLCWQLNRQMRLDF
                     RITNQIEIQLAKKNRDYFFTVYECIQPNKTLIHYLYNNQFDGEYLLPEFRHLDFFWLL
                     KGDDVSDELMNELTGSIRSISGVQLVTELTQTRIKHRENLIF"
BASE COUNT         1399 a         1323 c         1387 g         1467 t
ORIGIN      
        1 gatcccgggc cacctgctct tttgttctcc ttaatataaa ggggtataac aatttccgga
       61 gatggtcttt ccggtcgggt tccccgaact tgtcgatggg gatggcaaat tcctggcgga
      121 agaattctat gctgcccagc atgccgggat tcaggaaatt catctgcgca aagatgtcga
      181 aggtattgtt ctgcagggga gtaccgctca tgcataagcg atgtttggcg tgcagcaggc
      241 aggcggcctt ggtcaccttg ctcgatggat ttttaatggc ctgggattca tccagtacca
      301 cataatcgaa cgggatgtcc atgagcagct tgatatcgct gcgcaaggtt ccgtaggtgg
      361 tgatgagcac ttcaaatcca ttcagggcct ctttattgcg cagcctttcg ccgccgtggt
      421 gaatgcggta cgtaagactg ggggcaaatt tctttatttc attctcccag ttgaagatca
      481 gggtggtggg acaaacgacc agggccctta aatgcccgta gtgtccttta taatgatgca
      541 ggaaggaaag ggcctggatg gtcttaccca aacccatatc gtctgccagg atgcctcccc
      601 agttcacttc gtgcaaataa ttcagccagt ggaagccatg cacctggtag ggccggagcg
      661 tatgcacaag atgttccggc ggatcaattt cccggatggt cttaaattcc cggagttgct
      721 catatttttt ttccaggtgt atgatcagtt cttcctcatt ccggttttca tacaattcat
      781 caatcacgct caggtgatag cgggaaaggc ggagttcgtt gctcttgcct tcgcccacgc
      841 ggaaaagcag ggcatagcgt tttacccatt catccggcaa aaccccgagc gttccgtcat
      901 gcagcggaac aaattgctgt ttgttggcca gcgccttttt aacgtccgcg atagtcactt
      961 tctgatcgcc gaaaagaata tctacccgcg cgtcaaacca gtcggtgctg ctgctgatgt
     1021 gaatttttgt ctggggccgg gaagtattga aacggaaatt tttcagggca tcaaatccgt
     1081 gcacggtcac cttcatatcc tgcatcgcat ccatgaaaag gaagaaccag ttatttttta
     1141 atacatccgt tccccgcagt gccagtccgg aagagccctg cggctgaatg aactgggaat
     1201 gcagggactg cagaccggag ataaaagcgg attctttttc cttgttgcgc tggatgagca
     1261 ggaccttgtc gccgtccggc accacgagct cgtctttccc gctggccgtg gtctcaaatc
     1321 ccttataggt aaaaatgggc tggaacaaaa ggtattctcc tttttcctga agcaggatct
     1381 ttctctccgg atctccgctt ttgatttcct ttacgagttc cggatcaaac tccacatggt
     1441 attctttctg aagcggaagc acggttttac ggagctgctc cggccattcc ggtttcggaa
     1501 tgaacattct tccctccttg aatccggcaa ggacggcgat atcctccggt ttattccaga
     1561 ggtataaatt gttattatga aagaacagca cattgctttt gcacttgttc aggctgaggt
     1621 ctatcgattg gccggtgatg cgggtaaagc actttacttc aacctgattc ttttgaagac
     1681 tcactctaaa atatggggtc aggacatccg gcgaaatatt caccgattcc aggttggacg
     1741 ttttaaaagg cttatgcccg ttcagaaaaa aaataaacgg gttctccgag agttcggaaa
     1801 ataatttccg gaatttggga tgaaggtatt cgatgatcag cgccttggta tcgtccggca
     1861 gttcctcacc ttcagcgtgg ataatattct cccacatgcc gctgaaaggg gaattgcggt
     1921 tgaggtattt attcacctcc tgttcctggg ttttgcggat ggcatagacc agttgccggt
     1981 cgggttcgtt gaatttttct gtattgacaa accgcgccag ttccagtttt tccacttttc
     2041 cgacataggc cgtttgctgg tcattgcttt ctccgctgac ggcttccagt aaaaatccgg
     2101 gatacgcggc atgattaaaa ttaaatacca cgcccagccg ctgggctgcg cctgcgcgga
     2161 tctccggttc cagttcttcg gccaccaatt ccttttcggc agcaggctta ggtcttgacg
     2221 gaaaagccgg gctcacccgt ttgatggagc cgtcgagaac gcgcaaaaaa ggttttccct
     2281 ctttatagct gaactcaaat tttccatcga ggtcgtccga cagggaatat ccgtaagcct
     2341 ccagcagttt gtttttttct ttgtcccagt tgcggatggt atcgaaataa taggggccgt
     2401 tggactgcag caattgcaga aagagaatca ctttagggag gcaaagcgga tgggtggtgt
     2461 cgggataaga ggagctggta tcaaaattcc gctcttcgtt tttccggata agcaccggat
     2521 agtttgttcc gccgatacgc acattcgctt ttacggtttc atccgcagcg agttcgatgc
     2581 tggccttatt gccattcaga taatttcctg cttcttcata aacctccgcg gagcaaagca
     2641 gccggattgt tttgaggtca atggctttca tctttacgac cgtatgcgac tgatcgtaat
     2701 gaacttcttc cgctttcagc atattgcggt caagcaattc ctgcagctga ataagggccg
     2761 cggcctcgtg ccggcagata tcccccaggt tatagggaca actgcatctt accgagagcg
     2821 attttgcatc ccggaatttc tggacatata ctttataata ggtggaataa ctgtcgtctt
     2881 taatccggaa ggttaccgtt tcaaaaagct catcgtattc aatgagttcc acatatccga
     2941 tcgcgtgaat ttttttcccc cgccggatca cttcgtcggt tccattggtg tatacgtatt
     3001 taatcaggtg cggtaacgcc attcaacttg tctgtttacc attaacccgg tatccaagcg
     3061 gattcggaaa aggctaatct gcaaaagtaa agaaaaaacc catgcgggaa aagttttgga
     3121 aggtttttat ggaccctttt aagccggatg ggggaagccc agcctcgatt tcccgtcacc
     3181 cgggtgaaat ccattttccg ggaaggagtg gaatgtttgg aaatccggcc tggcagtatt
     3241 tttgcaaaat acggcccgcc ggaaaacgcg gtttgccgcc tcaacactca cgggttcctg
     3301 tatccgcagc ccacgcagaa tcaatggagg aaggccggga taaattcctt ttttaaaggc
     3361 tatttatact ttttggaata gcatttgccg ggcatacctt tgtatatggg atctcttcgt
     3421 aaaaccatct ccatgacgcg aagctttttt ctctttcttt tgtttttgaa tgcctccctc
     3481 ccttccgcct attcacaaag attgcgcaag gcggaccggc agctgatggc gcaaattcaa
     3541 accgatgtca cgtatttgtc gaatgataaa ctggaaggaa gaagagccgg aacgcccggc
     3601 gagaaactgg cttccgatta catcatttct tcctttgaaa aatccggttt gaaaccgatg
     3661 ggtgattccg gaacctggag gcagaccttt gaaatatatg acggcaggga tatcagccac
     3721 tgtgttttta ccataaataa tattccgctc attttaaaca gtgaatatat tccgctggca
     3781 ttcagtgccg cccggaaagt aaccggttca ccggccgtgg ccctgcagga aagcggctct
     3841 ccctggtttt acgacctgaa agacctgatg gatgccgcgc ataataatcc gcacgttgat
     3901 atggaaacag cgttgcgggc caaagccaaa gcgctcgcag gccgcggagc cagtgccgtt
     3961 attttctaca acagctcaaa ttcaaaagag gaagacggga ttgagtttaa tcaggcggaa
     4021 ggaattgttc cggaaaaaat tccggtggta tttgtaaccc gggagggaag aaaaaaatat
     4081 ttgaaggata tttcgcagac gctggacgtg gtcattgata taaaaatgga agagaaaaaa
     4141 agatgggggc ataatatcat cgggtatctg gacaaaggag ctccctatac ggtagtgatc
     4201 ggagcccact atgatcacct tggatttggg gaagacggaa actcgctgta ccgcggaaaa
     4261 gaaaaaatga tccatccggg ggccgacgac aatgcgagcg gtaccgccgc attaatggag
     4321 ctcgccggta tgctgcacaa atcaaaatca ttgaagagca attatctttt tattgcattt
     4381 tccggagaag aatccggttt gctcggatca aaatatttta cctggcatcc ggtggttgac
     4441 ctgtccaaaa tcagctatat gctgaacatg gatatgatcg gcagactgaa cgacagttcc
     4501 cataccctga tcattggcgg ctacggcact tctccgcaat ggtcctccgt gatcagcagc
     4561 agtgtaaata aaaaaatctt ttcactgcag ctggattcca gtggaacggg accgagcgat
     4621 catacttctt tttaccttaa ggatataccg gttttatttt tctttacggg gattcatccg
     4681 gattatcaca gaccgacaga cgccgcggcg cggatcaact atcccgggga agtgcaggtg
     4741 atcaaattga tttacagcat tacggaaaaa atggacggaa tgcctaagcc ggctttcagc
     4801 aaaaccaggg atacgcaata tggggaaacg ccggctttca atgttaccct gggcattatg
     4861 ccggattatt ctttcaccgg aaacggggtg cgcatcgatg ccatcagcga aggcaggccg
     4921 gctgaaaaag ccggattaca aacgggagat gtgatcatcc ggctgggtca gtaccccgta
     4981 aactcgctcc aaacctatat gcaggctttg tcccggttta accgggagga taaaacaacg
     5041 gttgaaatca gccggggaac agaagtaaaa accatggata tccagtttaa ctaatcgcgt
     5101 ccgatatgaa gctcaaactg aagaacggcg acctggaaga tgacttcttc gaagggtcgc
     5161 gcttactggg catagtggcg ccgctgaaag gctatcgtct ttgctggcag ctcaaccgcc
     5221 agatgagact ggatttccgg atcaccaacc agatcgaaat acagcttgcc aaaaaaaacc
     5281 gggattattt ttttacggtt tacgaatgta ttcaaccgaa taagaccctt attcattatc
     5341 tgtacaataa ccagtttgac ggggaatacc tgttgcccga attcaggcac ctggactttt
     5401 tctggttgct gaagggagat gacgtatcgg atgaactgat gaatgaactg accggatcga
     5461 tccgctcgat ctccggcgta caactggtaa ccgaactgac ccagacaaga ataaagcacc
     5521 gggaaaatct gatcttctga tcaactgttc atgctggcca gaaactcttc attgct
//
LOCUS       DASYCU010000543         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2582407, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000543 DASYCU010000000
VERSION     DASYCU010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582407"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..2010)
                     /locus_tag="VGM24_10995"
     CDS             complement(79..2010)
                     /locus_tag="VGM24_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY4155949.1"
                     /translation="MRTSSIALCFFLFLCLAETSAQVRLPRLISDGMVLQRDSRIKVR
                     GWAAPGEQVVLNFHGQTDTVRAGADGKWALELLPLKAGGPFSMEIRATNRIIIKNILI
                     GEVWLCSGQSNMVLPMERVKERYPAIIARAANPDIRQFFVPLHYDFNKPQDELPDGHW
                     TAADPQTVLQFSAAAYFFARALYEKYHVPIGLINASVGGSPVEAWMSEEALTEFPGEM
                     QRVEKFRNARFTDSIREADQRISTDWNELLQKKDSGLQEAIPWFDTAYDASGWKEMIV
                     PGYWDEQGLTLKNGVVWFKKEIRVPASMTGKPAKLLLGRIVDRDFVYVNGRLSGTTGY
                     QYPPRRYELPAGLLRPGRNEIVIRVISNSGRPGFIKDKPYQLTAGGKTIRLAGSWKYK
                     TGAGMPPLPPAVFLQYKPLGLYNGLIAPVTHYTIKGVIWYQGESNTGRAADYRHLFSA
                     MIKDWRKKWNQGDFPFLYVQLPNYGIPSVPLSGKSDWAELREAQLKTLDLPNTAMAVT
                     IDIGEWNDLHPLDKQDVGERLALAARNLAYGEKHLEYSGPLYQSMKVEGKKIILRFTH
                     TGTGLLAKGGELKAFAIAGADHQFLPASAKIVNGKLMVWNNKLAQPRSVRYAWADNPA
                     GANLYNKEGLPASPFRTNE"
     gene            complement(2100..>2687)
                     /locus_tag="VGM24_11000"
     CDS             complement(2100..>2687)
                     /locus_tag="VGM24_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155950.1"
                     /translation="TAIWMPDSATAAQAALRVPAFKGAIVPPGPTGSWTPAFYPSLKK
                     YDDSTRSSLNDASTRPYVMFRFSDVYLLAAEAYFQAGDNTNAAAMLNVLRQRAAYKAT
                     NTPQQNADAAIAQTILPSDVTLDFILDEYTREDYGDCQRWWDLVRTQSLIRRVQMYNP
                     EAAANIQPYHVLRPIPQTQIDLVTSGPAFPQNPGY"
BASE COUNT          621 a          721 c          693 g          652 t
ORIGIN      
        1 ataccgccct gcaaataaaa gcaaaaacgg ccaatccaat aaatcggtaa gaccaccctg
       61 acccgtaaag cccggtcctt attcattcgt tcggaatggc gaagccggca acccctcttt
      121 attatacaaa ttggctccag cgggattatc tgcccaggca tatcttaccg accgcggctg
      181 cgcaagcttg ttattccaga ccatcaattt tccatttacg atttttgccg atgccgggag
      241 gaattgatga tccgctcccg caatggcaaa cgctttgagc tctcccccct tcgccagcaa
      301 accggttccg gtatgggtaa agcggaggat aattttcttt ccttccactt tcatggattg
      361 gtataaggga ccggaatatt cgagatgctt ttctccgtaa gctaaattcc gcgcggccag
      421 ggcaagccgc tcgccaacat cctgtttatc gagaggatgc aggtcattcc attcgccgat
      481 atcgatggta accgccatgg cggtatttgg caggtccagg gttttcaatt gtgcttcccg
      541 cagttcggcc caatcgcttt taccggagag cggtacggag gggatgccat aattcggcaa
      601 ttgaacatat aaaaaaggaa aatcaccttg attccatttt ttcctccagt cttttatcat
      661 cgcggaaaac aagtgccggt aatcagcggc cctgccggta ttcgattcgc cctggtacca
      721 gatcacgcct ttaatggtat agtgagtaac gggggcaatc aatccgttgt ataggcccag
      781 gggcttgtat tgcagaaaga ccgcaggagg caggggaggc atgccggccc ctgttttata
      841 tttccaggag ccggcaagac ggatggtctt ccctcccgcc gtcagctgat acggtttgtc
      901 ctttataaaa cccggccggc ccgaattgct gatcacccgg atcacgattt cattcctgcc
      961 tggccggagc aggcctgcgg gcaattcata tctcctgggc ggatactggt aaccggtagt
     1021 tccggacagg cggccgttca catacacgaa atcccggtcc acaattcttc ccagtaaaag
     1081 tttcgccggt ttcccggtca tggaggccgg aacccggatt tcttttttaa accataccac
     1141 gccgttcttt aaggttagtc cctgctcgtc ccagtatccg ggaacgatca tttctttcca
     1201 gccggatgcg tcataggccg tgtcgaacca ggggatcgct tcctgtaagc ccgaatcttt
     1261 tttttgaagc agttcattcc agtctgtact gatccgctga tcggcttccc gtatgctgtc
     1321 ggtgaaacgg gcgttcctga atttttcaac tctctgcatt tctcccggaa attcggtcaa
     1381 tgcttcttca ctcatccagg cttcaaccgg cgatccgccc acactggcat tgatcaggcc
     1441 gatgggaaca tgatattttt catacagggc ccgggcaaaa aaataggcgg ccgcgctaaa
     1501 ctgcagcacc gtttggggat ctgcggctgt ccaatgtcca tccggaagct catcctgcgg
     1561 cttattgaaa tcatagtgga ggggaacaaa aaactgccgg atatccggat tggcggcacg
     1621 ggcaataatt gcgggataac gctccttgac ccgctccatc ggcaatacca tattggattg
     1681 cccggaacat aaccatacct cgccgatgag aatatttttt atgatgatcc ggttagtggc
     1741 ccggatctcc atgctgaagg gcccgcctgc cttcaaggga agtaattcca atgcccattt
     1801 tccgtcagct ccggcccgga ccgtatccgt ttgaccatga aaatttagaa ccacctgttc
     1861 acccggagcg gcccatccgc ggacctttat cctggaatct ctttgcaaca ccatgccgtc
     1921 gctgatcagg cggggaagcc ttacctgtgc ggaagtttcc gccaggcaca aaaataaaaa
     1981 aaagcaaagg gcaatggagg aggttctcat gatcactttt atcctcagaa agattgcagg
     2041 tggaagatgg ttcgttccac ctgcaatatg aaattacgcg aaatgaattg cagttgttgt
     2101 caataccccg ggttctgcgg gaaagcgggt cccgaagtga ccagatcgat ctgggtttgc
     2161 gggatcggtc ttaaaacatg gtagggctgg atattggcgg cggcttcggg attatacatc
     2221 tgcactctcc ggataaggga ttgggtccgc accaggtccc accagcgctg gcagtcgcca
     2281 taatcttccc gggtatattc atccaggata aaatccaggg tcacgtccga cgggagaatg
     2341 gtttgcgcta tcgccgcatc ggcattttgc tgcggggtat ttgtggcttt gtacgcagcc
     2401 ctctggcgga gaacattcag catggccgcc gcattggtat tatctcctgc ctgaaaataa
     2461 gcctcggcag ccaacaggta aacatccgaa aaccggaaca tcacgtaggg cctggtggaa
     2521 gcatcgttca ggctggaccg cgtggaatca tcatattttt tcagtgaagg ataaaaggcg
     2581 ggagtccagc tgccggtagg tcccggaggc acgatagctc ctttaaaggc aggcacccgg
     2641 agggcagcct gggcagcagt cgcagaatcg ggcatccaga tggccgt
//
LOCUS       DASYCU010000544         4541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5043557, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000544 DASYCU010000000
VERSION     DASYCU010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5043557"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="VGM24_11005"
     CDS             <1..1544
                     /locus_tag="VGM24_11005"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4155951.1"
                     /translation="KKKTMKTRYLIASFLLAALSAGLISGCSKQLKEDPKASLTPGTY
                     FQTQNDLDAAVAGMYAVLAHDGAWGFTNKMTTYFGADDLTTDPGLNKGNQREFDQLAG
                     ASDNPDIGAMWNGPWQTIYNANNIIANYGQVNSSDAQKNESAGQAYFLRGLCYYYLVR
                     TFGTLPLVLTTEAGAHPPRSSVDSIYALVISDLQQAKSMLGTSKTQGMPTTYSASAVL
                     ADVYLTMAGWPLNDATKYDMAATEAKSVMQSNVYDLSTPYDKVFTTNNCAESVFALEY
                     SVAGNLPNRSFGSTSVPLDEVATDGSSGWDDFYPEINFFKKAPACTRTDLTFYTTLKL
                     IQPDKSFVLTPWNSPTTHAQHPYYKKFRAGLNGDGVNETPTAINTIQPSTNKETIIVR
                     YPQVLLDFAEASAMASSGPTSESYDAINQVRARAGEPPLVTGLSATDFQDSVVSERAY
                     EFAGEFGMRWFDIVRLQLLNQITAGRDPAENPLTHPVDQTRYLAPIPYSEMVLNPNWK
                     QNDGY"
     gene            1671..3929
                     /locus_tag="VGM24_11010"
     CDS             1671..3929
                     /locus_tag="VGM24_11010"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEY4155952.1"
                     /translation="MKKLLLCGKIAGIFFLQSLFFSPVYAGLPPFLSDTSAPKSLGYI
                     DPTIGNIGRLLEPTRPTAQWPNQMIRVYPIRKDYLDDQISGFPLTAVSHRLGEVFSVK
                     PAIAPPDPAEWNRRMPYDHDLEITRPWYYSTYLIRDGITVEFTPGKKTGYYRFGFPAG
                     ENKSILLSAFNDDESSWHFLSGTAVEGEETWHGDTKIYMYGEFSTAGTVVVLENGELK
                     NGTFVSGKPARAGIRFPAQTKQVELKYAISFISASQAQRNFNEEIRHRDFNAMKKNAE
                     AAWSSVVSQIQVEGGTEAQRRSFYTALYRCHERMVDITEDSKYYSGFDKKIHSDPRPF
                     YVDDWSWDTYLALHPLRMLLHPKQEEDMLQSYVRMYEQSSWMPTFPVLFGDHACMNGF
                     HSSIIFLDAWRKGLRNFDVEKAFEGIEKNAMEATLLPWRNGPKTALDDFYQARGYFPA
                     LHPGEKETVEQVHPFEKRQAVAVTLGCSFDDWAAAQLATDLGKTDDYRLLGKRAMNYK
                     NLWNAEERFFLPKDAQGNWIKIDPKFDGGPGGRDYYDENNGWTYLWQVQEDIPGLMQL
                     MGGRKAFEERLDQLFREDLGRSKFEFWNKFPDATGLVGQFSMGNEPSFHIPYLYNYAG
                     TPWKTQKMIRLLLSLWFGDNVFGIPGDEDGGGMSAFVVFSSMGFYPATPGIPEYTIGS
                     PVFSRVTINLENGRTFTLIAQNSSVVNKYIQRASLNGKELNKPWFTHEQLMKGGTLEL
                     KMGPKPNKSWGN"
     gene            4117..>4541
                     /locus_tag="VGM24_11015"
     CDS             4117..>4541
                     /locus_tag="VGM24_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155953.1"
                     /translation="MNKYILCIAILLSIKINGSAQSYGLAFNSHEVVLEKRTAIDLSP
                     EDSFCFSKDFALAFEFRFIPNHQIYFGYLFRIISNDNRNIDLIYNQPSSSFKLITGEN
                     FSGISFTIDSLHLYKKWNLFSLKCDQDNHLIRCFVNGRL"
BASE COUNT         1156 a         1123 c         1173 g         1089 t
ORIGIN      
        1 taaaaaaaaa gacaatgaaa acaagatatt taattgcatc gttcctgctg gcagcccttt
       61 ccgcgggact gatttcgggc tgctcaaaac agctgaagga ggatcccaag gccagtctga
      121 cgccggggac ctattttcaa acgcagaatg acctggatgc ggccgttgcc ggaatgtacg
      181 ccgtgctggc ccatgacggc gcctggggct ttaccaataa aatgactacg tatttcggcg
      241 ccgacgacct caccacggac ccggggctga ataagggaaa ccagcgggaa ttcgatcagc
      301 tcgccggagc ctccgacaac cccgacatcg gagccatgtg gaacggaccc tggcagacga
      361 tctacaatgc caataatatc attgccaatt acggccaggt caattcgagc gatgcacaga
      421 aaaatgaatc ggcgggccag gcttattttt taagaggcct ttgttattat tacctggtca
      481 gaacattcgg tacccttccg ctcgtgctga ccacggaagc gggcgcccat ccgccacgca
      541 gttcggttga ttcaatctac gccctggtca tcagcgacct gcagcaggcc aaatccatgc
      601 tgggcacatc gaagacgcag ggaatgccga caacttattc ggccagtgcg gtgctcgccg
      661 atgtatacct gacaatggcc ggctggccgc tgaatgacgc caccaagtat gatatggcgg
      721 ctaccgaagc gaagagcgta atgcagtcaa acgtttatga tctcagcacc ccgtacgaca
      781 aagtatttac caccaataac tgcgcagaaa gtgttttcgc gctggaatac agtgtcgcag
      841 ggaatctgcc gaaccggagt ttcggatcca ccagcgttcc gctcgatgaa gtagcaacag
      901 atggttcgtc cggctgggat gatttttatc cggaaatcaa tttcttcaag aaagcgcccg
      961 cctgtacgcg gactgacctc actttttaca ccacgctgaa actgattcaa ccggataaga
     1021 gttttgtgct cactccctgg aattccccga caacgcatgc gcagcatcct tactataaaa
     1081 agttccgcgc ggggctcaat ggagatgggg tgaatgaaac gccgaccgcc atcaatacca
     1141 ttcagccgag caccaataaa gaaacgatca ttgtccgcta tccgcaggtg ttgctggatt
     1201 ttgcggaggc atcggcgatg gcctcaagcg ggccgacctc cgaaagttat gacgccataa
     1261 accaggtgcg ggcccgcgcc ggagaacccc cgctggtcac cggactaagt gccacggact
     1321 ttcaggattc ggtggtcagt gaaagggctt atgaatttgc cggggaattc ggcatgcgct
     1381 ggtttgatat tgtcaggctt caactgctga atcagatcac cgccggccgg gatcctgccg
     1441 aaaatccgct cacccatccc gtggatcaga ccaggtacct tgcgcccatt ccatattccg
     1501 aaatggtgct gaatcccaac tggaaacaga acgacggata ttgacgggct tatctctgaa
     1561 attttcggtt taaccatgca cggtccgtgc acggttaaac cgttttcgta atggatgcag
     1621 ccaggcgggt ttgccgtgca tttgtctttg cagtttctaa aacaagattg atgaaaaaat
     1681 tattattgtg cggtaaaata gcggggatat tttttttaca gtccttattc ttttcgcctg
     1741 tttatgcagg gttgccgcca tttttgagcg atacttctgc tccgaaatcg cttggttata
     1801 tagatcccac gatcggaaat atcggaaggc tgctcgagcc gaccaggccc accgcacaat
     1861 ggcccaatca gatgattcgg gtttatccga tccggaaaga ttacctggat gatcagatct
     1921 ccggttttcc gctaacagcc gtttcgcacc ggctcggaga ggttttttcc gttaaaccgg
     1981 ccattgctcc gcctgatccg gcagaatgga accggcggat gccttatgat catgacctgg
     2041 aaattacaag gccctggtat tactccacct acctgatccg ggatggcatc acggtggaat
     2101 ttacccccgg aaaaaaaacc gggtattacc ggtttggttt tcccgcggga gaaaataagt
     2161 ccatcctcct gagcgctttt aacgatgatg aatcgtcctg gcactttctt tccggtactg
     2221 cagtagaagg ggaggaaacc tggcatggcg ataccaaaat atatatgtat ggtgaattca
     2281 gtacggcagg aactgtggtg gtccttgaaa acggagagct taaaaacgga actttcgtgt
     2341 ccgggaagcc tgccagggcc ggtatccgtt ttcccgcgca gaccaaacag gtggaattga
     2401 aatacgcgat tagttttatc agcgcttcac aggcgcagcg aaattttaat gaggagatcc
     2461 ggcaccggga tttcaatgcg atgaaaaaaa atgccgaggc ggcctggtcg tccgtggtgt
     2521 cgcagataca ggtggagggt ggcaccgaag cgcaaaggcg gtcattctat accgcgcttt
     2581 atcgctgcca tgaacggatg gtggatatca ccgaagattc gaaatactac agcggctttg
     2641 ataaaaaaat tcacagcgac ccgcggcctt tttatgtgga cgactggtcg tgggacacct
     2701 acctggccct gcatcccctc cggatgctgc tgcatccgaa acaggaagaa gacatgctcc
     2761 agtcctacgt gcgtatgtac gaacagagca gctggatgcc tacatttccg gtgctgttcg
     2821 gagatcatgc ctgcatgaac ggctttcatt catccataat tttcctggat gcctggcgga
     2881 aagggctacg aaattttgat gtggagaaag cctttgaggg aatcgaaaag aatgcaatgg
     2941 aggctaccct gctgccctgg cgtaatggtc ccaaaacagc gctcgacgat ttttatcagg
     3001 ccaggggata ttttcctgcc ctgcatccgg gagaaaaaga aaccgttgag caggttcacc
     3061 ctttcgagaa acgacaggcc gtggcggtaa ccctcggctg cagttttgac gactgggctg
     3121 cagcacaact ggcgacggac ctgggaaaga cagacgacta ccgtttgctg ggcaaacgcg
     3181 ccatgaatta taaaaacctc tggaacgcgg aggaacgttt tttcctaccg aaagatgcgc
     3241 agggaaattg gataaaaata gatccgaagt tcgatggcgg cccgggcggc agggattatt
     3301 atgatgagaa taacgggtgg acctatctgt ggcaggtaca ggaggatatc ccgggcctca
     3361 tgcagctgat gggcggacgc aaagcgtttg aagaaagact ggatcagtta ttccgggaag
     3421 atctgggcag atcgaaattt gaattctgga ataagtttcc ggatgcaacc ggtctggtcg
     3481 gccagttctc catgggcaac gaaccaagct ttcatattcc ctatttatat aactatgcgg
     3541 gaacgccctg gaaaacgcaa aagatgatcc gcctgttatt gtcattgtgg ttcggcgata
     3601 atgtttttgg cattccgggc gatgaagacg ggggaggcat gtccgccttc gtggttttct
     3661 cctccatggg attttatccg gctacgccgg gtattccgga atacacgatc ggcagccccg
     3721 tattcagcag ggtaaccatc aacctggaga acggccggac atttacgctt atcgcgcaaa
     3781 acagttcggt ggtaaataaa tatattcagc gcgcttcgct gaacggtaag gaacttaata
     3841 agccctggtt cacgcatgag caactgatga aaggcgggac gcttgaactg aaaatgggac
     3901 cgaaaccgaa taagagttgg ggtaattagg tggatggtgg atggtgggtt gtggtgcctt
     3961 agcccgaatt gcattaccat cattattata gtgatatgat gcccactctg cgcatgcagg
     4021 gtggctgctc atttacgatc gtccatccac catccaccac ctccgattcc ctccggaaat
     4081 gattcttaaa aaagatcctt atttttacag ttgcttatga ataagtacat cctgtgcatt
     4141 gcgattctct tatccataaa aatcaacggt tctgcacaat cttatggtct ggcttttaac
     4201 agccacgagg ttgttcttga aaaaagaaca gcaatagatc tttcgccgga ggactcattc
     4261 tgtttttcga aggattttgc gctggctttc gaattcagat tcattcccaa ccaccagatt
     4321 tatttcggct atcttttccg catcatcagc aatgacaacc ggaatattga tctgatttac
     4381 aatcagccct cctcttcatt taagctgata acaggagaga atttttcggg catcagtttt
     4441 acaatcgaca gcctgcacct ctacaaaaaa tggaacctgt tcagcctgaa atgcgatcag
     4501 gacaaccatt tgatccgttg ttttgtcaat ggcaggctga t
//
LOCUS       DASYCU010000545         5432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5043754, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000545 DASYCU010000000
VERSION     DASYCU010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5043754"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VGM24_11020"
     CDS             <1..209
                     /locus_tag="VGM24_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HEY4155954.1"
                     /translation="QLLLKKLAHLDPLEFKDKRVVVKGCGEKGIAPFAYLEITRLLRP
                     VAKSIMYGEPCSTVPIFKAGSPSK"
     gene            complement(184..1551)
                     /gene="mgtE"
                     /locus_tag="VGM24_11025"
     CDS             complement(184..1551)
                     /gene="mgtE"
                     /locus_tag="VGM24_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY4155955.1"
                     /translation="MPNLKDIKNLIQNNRLTDFVKLNKLSSRDIIDFSNRYTNWAGKL
                     FQHLDIKHAARIFKFIRRKKQEIIIRSLPDEKAAELLNALQPDDRTAFLGMLPGSAVK
                     ELLKVLAPETRAETLELLGYPEGSVGRLMTPDYISIKSSNTVREVLDMIRQQGQVTEM
                     LNFLFVVDDNGVLIDDINLKEFLIVDPSLPVEKIMDRQFLALSVNDAQTETIAIFKNN
                     SRFALPVINESGVLLGVVTLDDVLRLAEKENTREFQRIGGSEALDEAYTSISFGHLVR
                     KRAGWLVILFLGEMLTATAMGFFEGEISKAVVLALFIPLIISSGGNSGSQASSIIIRA
                     MALGEIKFRDWLYVMRKEIFSGILLGSILGLVGFLRIVLWQRFRFYNYGEHWFKIGLT
                     VAFSLLGVVLWGTISGSMLPILLKKLGADPAASSAPFVATLVDVTGLIIYFSVAVLIL
                     RGSLL"
     gene            complement(1615..2772)
                     /locus_tag="VGM24_11030"
     CDS             complement(1615..2772)
                     /locus_tag="VGM24_11030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY4155956.1"
                     /translation="MILSIVIVNFNVKYFLEQCLCSIEKAVGQSILLAGNTEVFVVDN
                     ASTDGSVDFLEPLYPQFHFIRNRENSGFAKGNNLALAHCSGEYILFLNPDTILAEDSL
                     DLCISFLQANSGAGGVGVRMIDGRGAFLRESKRGFPGPRASFFKMSGLASLFPRSRVL
                     AAYYAGDLDPRHNHPVDILSGACMMVPKEVLDKTGGFDGQFFMYAEDIDLSYRIRKAG
                     FQNYYLASTSIIHFKGESTPRDAGRLKQFYFAMNQFLKKHFLDSSPSRMLFFLNRAVA
                     LKLRMEMIRLRFQKTPGDPARIDSVFIKGEPDNPDLLRNAFREAGIRLAETEAAAGGI
                     LFCESAGRSMKSIIREMDEAPRRLSFYIHGSGTHAAVGSSSQEEKGDVISW"
     gene            2843..3658
                     /locus_tag="VGM24_11035"
     CDS             2843..3658
                     /locus_tag="VGM24_11035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY4155957.1"
                     /translation="MERLPALYYLCAMLLKPTLIKAAQEAGKVLQHYYGGHFQVSHKE
                     GINNLVTEADHAAEKLIMEIIRAEFPDHYILSEESGDMPSASKYKWIIDPLDGTVNFA
                     HGLPLCCVSIGIEYDGEMQMGAVYNPFFNEFYFAQKGKGATLNDRTLQVSGETEVSKA
                     CLVTGFPYTYLDQPNGPLEVFSRLIRKGVPVRRLGSAAMDLCWVAAGRFDGFFEHKLN
                     AWDSAAGFLIVEEAGGRVTDYSGSRYSPYQPQIAATNGKIHDELLKWIDGSIM"
     gene            3664..4695
                     /gene="thiL"
                     /locus_tag="VGM24_11040"
     CDS             3664..4695
                     /gene="thiL"
                     /locus_tag="VGM24_11040"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY4155958.1"
                     /translation="MDSRTEISALGEFGLIDHLTQNIELKNASSILGVGDDAAVIDHF
                     GKQTVLTTDLLIENIHFDLMYTPLRHLGYKSVVANLSDIYAMNAIPTQILISLGFSNR
                     FSLEALDEFYEGVYAACERYDTDLVGGDTSSSQKGFIISITAIGEVSPDKFVQRGTAQ
                     KGDLICCSGDLGAAYLGLIFLEREKKIFIENPEIKPDLEGEKYVIGRMLKPEAAKETI
                     DFFEREGIVPTAMMDISDGLSSELLHICKKSGLGCVIYEEKIPIAEETRKAAFKFELD
                     PTACALSGGEDYELLFTVKPEDFDKLVLNERISVIGYMTGAEEGKKMISKGGNKFDIT
                     AQGWNAFTQ"
     gene            4713..>5432
                     /locus_tag="VGM24_11045"
     CDS             4713..>5432
                     /locus_tag="VGM24_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEY4155959.1"
                     /translation="MKTALKSGFEGMVWIICCALASCTASRQAFDPGRKYPPAKLQAD
                     YKIFRGILESAHPSLYWYTPKDSMDYYFNEGYSRLKDSMTEDQFRMDLSYVISKINCG
                     HTSVKASKAYIRYADTAEEKTFPFILKFWGDSMVVTANLRLQDREFKRGTILKSINGY
                     DAHQLTDTLFNFVTTDGYSLSGKYQTLSTGFTFANLYKHVMGFADSVDISYVDSLGRE
                     RQAYVPLYDFRSDTLRKRLQAE"
BASE COUNT         1441 a         1319 c         1374 g         1298 t
ORIGIN      
        1 gccagttatt gctgaaaaag ctcgctcacc tggatcccct ggaatttaaa gacaaacggg
       61 tggtcgtaaa ggggtgcggc gaaaaaggga ttgccccctt tgcctatctt gaaatcaccc
      121 ggcttttgcg gccggtagcg aaaagtatca tgtatggcga accctgcagt acggttccga
      181 tctttaaagc aggctccccc tcaaaataag aacggctacg gagaaataaa tgatcagccc
      241 ggtcacgtct accagcgtgg ccacaaaagg tgccgaagag gcagccggat cggccccgag
      301 ctttttcagc aggatgggca acatggaacc ggagatagtc ccccataaaa ccacaccgag
      361 taaagaaaaa gcgaccgtta aaccgatctt gaaccaatgt tctccataat tataaaaccg
      421 gaaccgctgc cataatacaa tccggagaaa accaaccaaa cccaggatgc tgcccagcaa
      481 tatgccggaa aaaatttctt tgcgcatcac ataaagccag tccctgaatt taatttctcc
      541 gagtgccatg gcccggatga taatggagga agcctgcgag ccggaattcc ccccgctcga
      601 aatgatcagc gggataaaaa gcgccagcac caccgccttg ctgatctccc cctcaaaaaa
      661 gcccatggcc gtagccgtta gcatttcccc caggaaaagg atcacgagcc agcctgcgcg
      721 ttttctaacc aggtggccga aagagatcga ggtataggcc tcatccaggg cttcggaacc
      781 cccaatccgc tggaactccc gcgtattttc tttttccgcc agccgcaata catcatccag
      841 cgtaaccact cccaggagca ccccggattc attgataacg ggaagggcga accggctgtt
      901 atttttaaaa atggcaatcg tttcggtttg tgcatcattt acagagaggg ccaggaattg
      961 ccggtccata attttttcca ccggcaggga agggtcaaca attaaaaatt ccttcaggtt
     1021 gatatcatcg atcagcacgc cattgtcatc caccacaaag agaaagttca gcatttcggt
     1081 tacctgtccc tgttgcctga tcatgtccag tacttcccgg accgtattgc tggatttaat
     1141 actgatataa tcgggagtca tcaaacggcc gacggatccc tcgggataac ccagaagttc
     1201 aagggtttct gcccgggtct ccggggccag cactttcagg agctctttaa cggcgctgcc
     1261 gggaagcatt cccaggaaag cggttctgtc gtccggttgc aatgcattca gcagttcggc
     1321 cgctttttcg tcgggcaggg accggataat gatctcctgc tttttgcgcc ggatgaattt
     1381 gaagatccgg gcggcatgtt taatatccag gtgctgaaac agcttccctg cccagttggt
     1441 ataccggttg gagaaatcaa taatgtcccg ggacgaaagt ttattgagct ttacgaaatc
     1501 ggtgaggcgg ttattctgta tcaggttttt aatatccttc agattcggca tacctcgtat
     1561 ttaatggttt acaatttgtt tttacccaaa aatgtttttg atcagatggc ttttttacca
     1621 ggaaatcaca tcccctttct cttcctgcga cgagcttccc acagcagcat gtgttcctga
     1681 gccatgaata tagaaggaaa gtctgcgggg agcctcatcc atttcgcgga taatggactt
     1741 catggatctt ccggccgatt cgcaaaaaag aattcctccg gcagcggctt ccgtttctgc
     1801 caggcggatc cctgcttccc ggaaggcgtt ccggagaagg tccggattat ccggttctcc
     1861 cttgataaat acagaatcaa ttcttgcggg atcgccgggc gtcttctgaa agcgaagtct
     1921 gatcatttcc atccgcaatt tcagcgcaac agcccggttc aggaaaaaaa gcatccggga
     1981 aggggaggaa tccaggaaat gctttttcag gaattgattc atcgcaaaat aaaattgttt
     2041 cagccggccg gcgtcccgtg gcgtactttc tcccttaaaa tgaataatgc tggtagaggc
     2101 gagataataa ttttgaaatc ctgctttccg gatccggtag ctcaggtcga tgtcctctgc
     2161 atacataaag aactgtccgt caaatccgcc ggtcttgtcc agtacctctt tcggaaccat
     2221 catgcaggcg ccggataaaa tgtcaaccgg atgattatgc cggggatcca ggtcgcctgc
     2281 ataataagca gccagcacgc gggaacgggg aaatagggac gccagtccgc tcatcttgaa
     2341 aaacgaagcc cgggggcccg gaaatccgcg tttggattct cttaaaaagg ctccgcggcc
     2401 atcgatcatt ctgaccccaa ccccaccagc cccggaattt gcctgaagaa aagaaataca
     2461 taggtccagg ctgtcttctg ccagaatcgt atccggattc aggaataaaa tatattcacc
     2521 ggaacaatga gcaagggcca ggttatttcc ttttgcaaac cctgaattct cccggttgcg
     2581 aataaaatgg aactgcggat aaagaggctc cagaaaatcc acgcttccgt ccgtggaagc
     2641 attatccact acaaaaactt cggtgtttcc ggccagcaga atgctctggc cgaccgcctt
     2701 ttcaattgag caaaggcact gttccagaaa atattttacg ttgaaattga cgatgacaat
     2761 ggaaaggatc atccgggata aacattagca cgaagtacga ttttttcagg tatccggcaa
     2821 aatagccctt taacgccatt ccgtggaaag gctcccggcc ctttattatc tttgcgccat
     2881 gttattaaaa ccaaccctga tcaaagccgc gcaggaagcg ggtaaagtgc ttcagcatta
     2941 ttatggcggt catttccagg tctcccacaa agaagggatc aataatctcg ttacggaagc
     3001 ggatcatgcc gcagaaaaac tgatcatgga gatcatccgg gcggaatttc cggaccacta
     3061 tatcctgagt gaggaatccg gagatatgcc ttcggcctca aaatacaagt ggatcatcga
     3121 tcccctggat ggcacggtta attttgccca cggccttccc ctgtgctgcg tgtccatcgg
     3181 aatagaatat gatggcgaaa tgcagatggg ggccgtttac aatccgtttt tcaacgagtt
     3241 ctattttgcc caaaagggga agggagccac actgaacgac cgcacgctgc aggtgtcggg
     3301 cgaaacggag gtttcgaaag cctgcctggt taccggcttt ccctatacct acctggacca
     3361 gcccaacggt ccgctggaag tattctcccg gctgatccgc aaaggagttc ccgtacggag
     3421 gctgggatct gccgcaatgg acctttgctg ggttgctgcc ggccgctttg acgggttttt
     3481 tgaacataag ctgaatgcct gggatagcgc agcgggattc ctgattgttg aagaggccgg
     3541 aggaagggta accgattatt ccggatccag gtattccccc taccagccac agatcgccgc
     3601 caccaacggg aaaattcatg acgagctgct gaaatggatt gatggaagca taatgtaaaa
     3661 actatggact cgcgcacgga aatctcggct ctgggtgagt ttggtctgat tgaccacctt
     3721 acacagaata ttgaattaaa gaatgcctcc tccatacttg gcgtgggcga tgatgcggct
     3781 gtaattgacc attttggaaa gcagactgtt ttaacgaccg atctgctgat agagaatatc
     3841 catttcgatc tgatgtatac gccgctccgg cacctggggt ataaatcggt ggttgcaaac
     3901 ctgagcgata tctatgccat gaatgccatt cccacccaaa tcctgatctc cctgggattt
     3961 agcaaccggt tcagcctgga ggccctggat gaattttatg aaggggtata cgcggcctgc
     4021 gagagatatg atacggatct ggtgggcgga gatacctctt cttcacaaaa gggattcatt
     4081 atttccataa cggctatcgg ggaagtgagt cccgataaat ttgtccagcg cggcacggcg
     4141 caaaaaggcg acctgatttg ttgcagcgga gatctgggcg cagcttacct tggccttatt
     4201 tttctggaac gcgaaaaaaa gatctttatc gagaaccccg aaataaaacc agacctcgaa
     4261 ggggagaaat atgttatcgg ccggatgctc aagcccgaag cggcaaaaga aaccatcgat
     4321 ttttttgaaa gagagggaat tgttccgacg gctatgatgg atatcagcga cggactgagt
     4381 tccgaactcc tgcatatctg taaaaaaagc ggactgggct gtgtgatcta tgaagaaaaa
     4441 attcccattg ccgaagaaac ccggaaggcg gcttttaagt ttgagcttga tccgacagcc
     4501 tgcgcgctga gcgggggaga agattatgag ttgttgttca ccgttaagcc ggaggatttt
     4561 gacaaactgg ttttaaacga aaggatcagc gtgatcggat atatgacggg tgcggaggag
     4621 ggaaagaaaa tgatcagcaa aggagggaat aaatttgaca tcacagcaca gggctggaat
     4681 gcgtttaccc aatgaatatt ttccgttttc tgatgaaaac agctctgaaa tcaggatttg
     4741 aggggatggt ttggatcatt tgctgcgcat tggcgtcctg tacggcgtcc cggcaggcgt
     4801 ttgatcccgg aagaaaatat ccgcccgcaa aacttcaggc ggattataag atattccgcg
     4861 gcatactgga atcggcccac cccagcctgt actggtatac accgaaagac agcatggatt
     4921 attatttcaa cgaagggtac tccaggctga aggattccat gacagaggac cagttcagaa
     4981 tggacctgag ctatgtaatt tccaagataa attgcgggca taccagtgtg aaggcttcca
     5041 aagcctatat ccgttatgcg gacacggcgg aagagaagac ttttccgttt atcctgaaat
     5101 tctggggcga ttccatggtg gttactgcaa atctcaggct ccaggaccgg gaattcaaaa
     5161 gagggactat tttgaaaagc atcaacggat atgatgcgca ccagctgaca gacacccttt
     5221 ttaattttgt caccacggac ggttatagcc tgtcggggaa atatcaaacg cttagcacgg
     5281 gatttacttt tgccaatttg tataaacacg tcatggggtt tgcggattcg gtggatatct
     5341 cttacgtgga cagcctggga agggaaaggc aggcttatgt gcccttatat gatttcagga
     5401 gtgataccct tcgtaaaagg ctgcaggctg ag
//
LOCUS       DASYCU010000546         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1858738, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000546 DASYCU010000000
VERSION     DASYCU010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858738"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1302)
                     /locus_tag="VGM24_11050"
     CDS             complement(37..1302)
                     /locus_tag="VGM24_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155960.1"
                     /translation="MDSKIAKNILLISFAAETFSASYGLLLGNDTALISILYFIAGLG
                     LVLSILFLPEARLDLRRSSARPAVWIKCALLAGMIFLAYITSRYWFGQIPVDPDYADM
                     LPVIKVMNERFLSGHWKQVYDPIQEIWNGTRPIYLPAMWLPYSGALLLKLDMRWVTSL
                     SLLLSFTAFLFILKIRKNNPYPYLLMAVAGMLFWWIFSRDDVHGLISLSEEGPVILYY
                     VLLCFAIVSGNIYFIALTASLCLLSRYSLIGWVIPFLLILLFKRQYRKGIVFSLAGIG
                     FFLIFFLIPFGAGPVRQLIRLPSGYVAFASRVWRDSPEVFWLNLGFAKFFGPHRTAAL
                     HQTLILCSFAVPVIFVLVCLYQKKRQWNNISLATLKLTLIVFLQLIDVPYGYLFYTSS
                     FVSLTILGILFSNFPDGTGSQYPPSASSL"
     gene            1387..2136
                     /gene="fabG"
                     /locus_tag="VGM24_11055"
     CDS             1387..2136
                     /gene="fabG"
                     /locus_tag="VGM24_11055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY4155961.1"
                     /translation="MKLLEGKIAIITGAARGIGEAIALKLASHGAHIAFTFLSSEEKA
                     RALEQKLIALGVKAKGYKSNAASYEDCEQLVNAVLKEYGTVDVCINNAGISKDSLLLR
                     LAPEQWDEIMNSNLKSVFNMTKQVIRPMMKAKKGSIVNMSSIIGIRGNAGQSSYAASK
                     AGIIGFTKSIAHELGSRNIRCNAIAPGFIETDMTHYLQDEEKANSFLAKIPLGRFGSS
                     EEIADAALFLASGMSSYMTGQVISICGGLNI"
     gene            2177..2824
                     /locus_tag="VGM24_11060"
     CDS             2177..2824
                     /locus_tag="VGM24_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY4155962.1"
                     /translation="MTSIEKLLVANKNWATEKKTVDPRYFEKLSGIQTPGFLWIGCSD
                     SRVPANEITGTQPGEIFVHRNVGNLVIHTDVNLLSVLEYAVVHLQVKHVIICGHYGCG
                     GISAAMTNHDFKQVLNMWLRNIKDVYRLHRSELEAISDLKKREDRLTELNVQEQVLHL
                     AKTSIIQRAWQHTKAPQLHGWVYGLNDGLLHSIFSMDPGSPLDPLYTFDDLSKEG"
     gene            complement(2832..>3184)
                     /locus_tag="VGM24_11065"
     CDS             complement(2832..>3184)
                     /locus_tag="VGM24_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY4155963.1"
                     /translation="ELAIGIGPFVIGPAMERKIGSSAFSQLAIDSSSWRTAEWAKSRG
                     FYSEVHASRELMDESVARLITHLGHSNPDAMKEMKKMLWEGTSHWDQLLHERAKISGK
                     LVLGAFAQKAINKG"
BASE COUNT          919 a          760 c          795 g          710 t
ORIGIN      
        1 ccggctctgc aacgaccgga cgtatatgga tgagattcac aagctgcttg ccgaaggagg
       61 atactgactt cccgttccat ccgggaaatt gctgaagagg attcccagga tcgtaagact
      121 cacgaaagaa gaagtataaa aaagatatcc gtaagggaca tcgatcagtt gaagaaatac
      181 gatcagggtc agttttagcg tggccaggct gatgttattc cattgccgct ttttctgata
      241 caggcaaacc agcacaaaaa ttaccggcac cgcaaaactg cagagaatca gggtctgatg
      301 cagggctgcc gtccggtgag gtccgaaaaa tttggcaaat cccaggttaa gccagaatac
      361 ttcgggggag tctctccata ctctcgacgc aaaagccaca taaccggaag gaagccggat
      421 cagctggcgg accggaccgg cgccgaaagg aatcaaaaaa aatatcagga aaaatccgat
      481 tccggcgagc gaaaaaacaa tccccttccg gtattgcctc ttaaagagca ggatcagcag
      541 gaagggaatt acccaaccaa tcagactgta ccggctcagc aggcaaaggg aagcggtcag
      601 cgcaatgaaa taaatatttc cggacacgat ggcgaagcaa agcaaaacat agtataaaat
      661 aaccggccct tcctcactca ggctgattag tccgtgcacg tcatcccttg aaaaaatcca
      721 ccagaagagc atgccggcaa cagccatcag caaataggga tacgggttat ttttcctgat
      781 cttcagaata aaaaggaagg cggtgaaact gagcagcagg gaaaggctgg tcacccatcg
      841 catatccagt ttcagcaaca gcgctccgga ataaggcagc cacatagcgg gaagatagat
      901 cgggcgggtg ccattccata tttcctgtat gggatcgtac acctgtttcc agtgaccgct
      961 caggaaccgt tcattcatca ctttgatcac cggcagcatg tccgcataat ccgggtcaac
     1021 cgggatctgg ccgaaccagt agcgggaggt gatataggcc aggaagatca tccctgccag
     1081 tagcgcgcat tttatccaga cggccggccg tgctgaactt ctgcggagat ccagccgggc
     1141 ctccggaagg aataagatgg agagaaccag gcccaggccg gcaataaagt acaggatgga
     1201 aatgagcgcc gtatcattac ctaaaagaag gccgtaactg gcagaaaaag tttccgctgc
     1261 aaatgaaatc agtaatatgt tttttgcaat tttagaatcc atggccgccg gaaagcgtta
     1321 aattaatatt tttatttacc tgcaaacttc cgagtgtata tatttgacat tctttaatgc
     1381 aatgaaatga aattattaga gggtaaaata gccatcataa ccggagctgc ccggggaata
     1441 ggggaagcaa ttgctcttaa actggcatcc cacggagcac acattgcatt tacttttcta
     1501 tccagtgagg agaaggccag agcactcgaa caaaagctga tcgcactggg ggtaaaagca
     1561 aaaggatata aaagtaatgc ggcttcatat gaagattgcg aacagctggt aaacgccgta
     1621 ctgaaagaat atggaacggt cgatgtttgc ataaataatg cgggcatttc caaagatagt
     1681 ttgttgttgc gtcttgctcc cgaacaatgg gatgagatca tgaacagcaa tctgaaaagt
     1741 gtattcaata tgaccaagca ggtgatccgg cccatgatga aagcaaaaaa gggatccatt
     1801 gtaaatatga gttcgatcat tggtatccga ggcaatgccg ggcaatccag ctatgccgca
     1861 tccaaggcag ggatcatcgg ttttaccaaa tccattgccc acgaattggg cagccgcaat
     1921 atccgctgta atgccattgc gccgggattt attgaaaccg atatgaccca ttatctccag
     1981 gatgaagaaa aagccaattc gtttctggcc aagattcccc tgggaaggtt cggcagttcc
     2041 gaggagattg cggatgccgc ccttttcctg gcttccggaa tgagcagtta tatgaccggt
     2101 caggtgatca gcatttgcgg aggactgaat atctgatcca tccgctttcc cttttcaccg
     2161 gattaaaatg caggatatga cttcaataga aaaattattg gtcgcgaata aaaactgggc
     2221 gaccgaaaaa aaaacagttg atccccggta ttttgaaaaa ctctcgggaa tacaaacccc
     2281 cgggttcctc tggatcggtt gcagtgacag ccgggtgccg gccaatgaaa tcacgggcac
     2341 ccagcccggt gaaatcttcg ttcaccggaa tgtaggaaac ctggtgatcc atacggatgt
     2401 gaatctgctc agcgtgctgg aatatgcggt cgttcatctc caggtaaaac acgtgatcat
     2461 ttgcggccat tacggatgcg gcggtatcag cgcggccatg accaatcacg atttcaaaca
     2521 ggtgctgaat atgtggctcc ggaatattaa agatgtctac cggctgcacc gcagcgagct
     2581 ggaagccatt tccgatctga aaaaaagaga ggaccggctg acggagctca atgtacagga
     2641 acaggtatta cacctcgcca agacgtccat tatacaacgg gcctggcaac atacgaaagc
     2701 cccgcaacta cacggatggg tttacggttt gaatgatggc ctgctgcatt ccattttcag
     2761 catggatccg ggttcgccgc tggatccctt gtatacgttt gatgatttga gcaaagaagg
     2821 ttaaggattt attatccctt attgatcgct ttctgcgcaa aggcccccag gaccagcttc
     2881 ccgctgatct tggcccgttc atgaagtaac tgatcccaat gagaagtgcc ctcccataac
     2941 attttcttca tttctttcat ggcatccgga ttggaatgac ccaggtgcgt gatgagccgc
     3001 gccacggatt catccatcag ttccctgctt gcatgcactt ctgaataaaa acccctggat
     3061 tttgcccact ccgccgttct ccaggaagag gagtctatgg caagctggga aaacgccgat
     3121 gaaccgatct tccgttccat ggcgggtcca ataacaaagg gtccgatacc aatggccagt
     3181 tcgc
//
LOCUS       DASYCU010000547         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1427138, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000547 DASYCU010000000
VERSION     DASYCU010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427138"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /gene="lepA"
                     /locus_tag="VGM24_11070"
     CDS             <1..1535
                     /gene="lepA"
                     /locus_tag="VGM24_11070"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY4155964.1"
                     /translation="VSRALAACEGALLLVDASQGIQAQTISNLYLAIENDLEIIPVIN
                     KIDMDGAMVEEVTDQIIELIGCKKEDILLASARTGVGVDAILEAIVDRIPPPKGNPDI
                     PLQALIFDSVFNSFRGIIVYYRILNGVLRKGDRIRFVSTGLDYQADEIGILKMGLVPK
                     PELRAGDVGYIITGIKNAKEVKVGDTLTLSSNPDPQMIQGFEEVKPMVFAGIFPINTE
                     DFEELRECMDKLQLNDASLTYELETSQALGFGFRCGFLGMLHMEIIQERLEREFNQTV
                     ITTVPNVSFIAHTSNGIRIQVNNPSEMPDPTRIDRIEEPYIKSQIITKPEYIGNIITL
                     CLGKRGILLDQNYLTPTRVELHFELPLTEIVFDFYDKLKSQTRGYASFDYHPIGYRDS
                     DISKMDILLNGDKVDALSALIHRSRAQDFGRKLCEKLKELLPRQQFQIAIQAAIGSKV
                     IARETISALRKDVTAKCYGGDISRKRKLLEKQKEGKKRMRQIGNVEIPQEAFLAVLKL
                     DE"
     gene            complement(1563..2543)
                     /locus_tag="VGM24_11075"
     CDS             complement(1563..2543)
                     /locus_tag="VGM24_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4155965.1"
                     /translation="MAKIRKSFFPILFIVFSGVFNQASAQRLSPAEKASLDLRQDSLK
                     SYAYNIVNAPEANERFRSDSFFVRMLVRALKIPNSFYYPFDSLQSISKLYAPDSSFRI
                     FTWQVKKDEMTYMQRGAIQRRTPDGSLKLTGLHDVSMFTARPLDSIRTANNWIGAIYY
                     RIIEKSVQGKNIYTLLGFDSYNFASNKKWMEVLSFDENGQPVFGGPYFSFRNDSAKAS
                     KKAVLRFNIEYKKEAATTFNYDSTLDMIIYDNLISESNEPTRKDTYVPDGDYEGFKWQ
                     NGQWVHVVKVFTTSLKDGQYPQDLKILNDAGGIDENRLRQQSEENRKKKK"
     gene            complement(2619..>3147)
                     /locus_tag="VGM24_11080"
     CDS             complement(2619..>3147)
                     /locus_tag="VGM24_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY4155966.1"
                     /translation="SWTIFLDRDGVINYENPGNYVRNREEFRFYPDTPENIAFFNSRF
                     QRVILATNQRGIGKGLMSIADLEDIHRHMQEQIGQKGGRIDRIYYCLDMESESPCRKP
                     NPGMARQAVQDFPEINLTRSVMVGNNISDMGFGRNAGMFTVFLSTTNPGMIFPHEWVD
                     LHYADLSEFARALKS"
BASE COUNT          807 a          776 c          773 g          791 t
ORIGIN      
        1 aagtcagtcg tgccctcgcc gcctgcgaag gagcgttatt actcgtggat gcctcccagg
       61 gcatccaggc acagaccatt tccaacctct atctggccat tgaaaacgat ctggagatca
      121 ttccggtgat caataaaatt gatatggacg gcgccatggt ggaagaagta acggaccaga
      181 tcatcgaact cattggctgt aaaaaagaag acatactgct tgccagtgcc cgcaccggcg
      241 tgggcgtaga tgccattctc gaagccattg tagaccgcat tccgcctccg aaaggcaatc
      301 cggacattcc tttgcaggcc ctgatctttg acagcgtatt caattctttc cgggggataa
      361 tcgtttatta ccggatcctg aacggggtat tgagaaaggg cgaccggatc cggttcgtct
      421 ctaccggcct cgactatcag gcggacgaga tcggcatctt aaaaatgggc ctggtaccta
      481 aacccgagct gcgcgcgggc gatgtgggat acatcattac cggtatcaag aatgccaaag
      541 aagtcaaggt gggagatacc cttacccttt cttccaatcc cgatccccaa atgatccagg
      601 gatttgaaga agtgaagccc atggttttcg ccggcatttt cccgatcaac acggaagact
      661 ttgaagagct gcgcgaatgc atggataaac ttcagctgaa tgacgcgtcc ctgacctatg
      721 agctggaaac atcacaggca ctgggatttg gtttccgttg cggatttctg ggcatgctgc
      781 acatggagat catccaggaa aggctggaac gggaattcaa ccagaccgta atcaccacag
      841 ttccgaacgt gagctttatt gcccatacca gcaacggaat caggatccag gtgaacaacc
      901 cttcagaaat gcccgatcct acccggatag accggatcga ggaaccctat attaaatccc
      961 agatcattac caagccggaa tatattggga atataatcac tttatgttta ggtaaacggg
     1021 gtattctgct ggatcagaat tacttaactc caacgcgggt ggagctacat ttcgagttgc
     1081 ccctgaccga aatcgtcttc gacttttatg ataagctgaa aagccagacc cggggctatg
     1141 cttctttcga ttatcatccc attggttacc gcgacagtga tatttcgaaa atggatattc
     1201 tactgaacgg ggacaaagtg gatgccctca gcgccctgat ccaccgcagc cgtgcccagg
     1261 atttcggccg caagctctgc gagaagctga aagagctgtt accccgccag caattccaga
     1321 tcgccattca ggcagccata ggctccaaag tgatcgcgcg tgaaaccatc agcgcgctaa
     1381 gaaaagacgt taccgccaaa tgttatggcg gcgatatatc ccgcaaacgc aagctcctgg
     1441 aaaagcagaa ggaaggaaag aaacgtatgc ggcagatcgg aaatgtggaa attccccagg
     1501 aagcttttct ggccgtgctg aaattggatg agtaggaatt ttcctttctt tcccaattaa
     1561 ccttatttct tcttcttcct gttctcctcc gactgctgtc ttaatctgtt ttcgtcaatc
     1621 ccgccggcat cattgagaat tttaagatcc tgcggatact gtccgtcttt cagtgaggtg
     1681 gtgaatacct tgaccacgtg aacccattgg ccgttctgcc atttgaagcc ttcgtaatcg
     1741 ccatcgggga cataagtgtc ctttcgggtc ggctcattgg attcggaaat gaggttatca
     1801 taaatgatca tgtcgagggt cgaatcgtaa ttgaaagtgg tggcggcttc tttcttgtat
     1861 tctatattaa atcggagcac ggcctttttg gatgcttttg ccgaatcgtt cctgaaagaa
     1921 aaatacgggc ctccaaacac gggttgcccg ttctcgtcaa agctgagcac ttccatccat
     1981 tttttattgc tggcgaaatt gtagctgtca aatcccagca gggtataaat gtttttcccc
     2041 tgaacggatt tttcgatgat ccggtaatat attgcgccga tccagttatt ggcagtgcgg
     2101 atcgaatcca ggggtcgggc cgtgaacatg gaaacatcat gtaatccggt cagtttaagg
     2161 gacccatccg gagttctccg ctgaatggct ccccgctgca tataggtcat ttcatctttc
     2221 tttacctgcc aggtaaatat gcgaaaactg ctgtccggcg catacaattt ggaaatggat
     2281 tgcagcgagt cgaaaggata ataaaaagaa ttcggaattt tcagcgctct taccagcatg
     2341 cggacaaaaa aactgtctga acggaaacgc tcattggctt cgggggcatt gacaatatta
     2401 taggcataac ttttcaggga atcctgccgg agatccagcg atgccttttc agccggtgaa
     2461 aggcgctggg ccgaagcctg attaaaaacg cctgaaaaaa caataaaaag aataggaaaa
     2521 aagcttttcc ggatcttggc cataaagttg aaattcgtac tactaaatta caggtaagaa
     2581 caggaactct ccgttaacgc cggaattctt agaaaatttt atgatttcag cgcccgggcg
     2641 aattcgctta aatcggcata atgtaaatcc acccattcgt gggggaaaat catcccggga
     2701 ttcgttgtcg agagaaaaac ggtgaacatg ccggcatttc ttccaaagcc catgtcactg
     2761 atattgtttc ccaccatcac ggaccgggtc aggttgatct ccgggaaatc ctgaaccgcc
     2821 tggcgggcca tgcccgggtt tggtttgcgg caggggcttt ctgattccat atccaggcaa
     2881 tagtagatcc ggtcgatgcg gccgcctttt tgcccgatct gctcctgcat atggcggtga
     2941 atgtcttcca ggtcggcaat gctcatcagt cctttgccga taccccgctg attagtggcc
     3001 aggatcaccc gttgaaaccg gctgttgaag aaggcaatat tttctggggt atcggggtag
     3061 aacctgaact cttcccggtt ccgcacgtag tttcccgggt tctcataatt gatcaccccg
     3121 tcccggtcaa ggaaaatggt ccagctt
//
LOCUS       DASYCU010000548         2798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4898868, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000548 DASYCU010000000
VERSION     DASYCU010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4898868"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..2232)
                     /locus_tag="VGM24_11085"
     CDS             complement(67..2232)
                     /locus_tag="VGM24_11085"
                     /inference="COORDINATES: protein motif:HMM:NF039873.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY4155967.1"
                     /translation="MHLTTRPKIGLALSGGGAKGLAHIGILKAIDSAGLSVDYITGTS
                     MGSIIGSLYASGYSGDSIEKVAGNIDWDLMLSNQSSLRNIIMEEKDEYGKYDLELPWV
                     NHFFRLNTGVLEGQELWLKFSELLFSVHQIKDFNQLPIPFKCIATDLSTGEAVVLDSG
                     ELVSAIRASMAIPSVFTAVDYNGKRLVDGGVVRNFPVKDLRDMGAQIVIGSNVSQGLL
                     AANKVNNALQVLLQVAFFREAEDTKLEVPLCDIYVQTPLEKFNMGSFSQADEIIEAGI
                     EEGRKLYPRLKRLADSLNAIYGPPQPKRKMPGADSVLITSYAVRGLKHTTENFFIHTM
                     GLLTHRRYTPENLAQMIRREAGTRYYNRIVYFLDPQEDHSSKIIFDVSENPLSFGKVA
                     LHYNQFSGISAILNLTTRDFFTPSSRSMATIDIGENFRIRGEHLQYFGRGRKFAFGLS
                     TQFDQFNITSYNDTKEAGIYNENYFVSDARIGYSTNRNLTFAAGSRFEWLRYKPSISS
                     TLVFKGTNDFLTSYVYLKHNSLDKPVYPRKGVKTELEGDWVYQQNPDIQLHFNNLDMD
                     TVASRSPYPRLLFHFESYTPLSSRSTLLFNLQSGINFRYNNNIMNEFSIGGLTSSFHN
                     QITFAGLREGSFYSASVAATQLGFRYQLFNNIFLTGRANMLFNNFVSKAQFYNSPDFL
                     SGYALTFTYNFALGPLEISAMYCDQWKRVMGYLNIGIPF"
     gene            complement(2389..>2798)
                     /locus_tag="VGM24_11090"
     CDS             complement(2389..>2798)
                     /locus_tag="VGM24_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4155968.1"
                     /translation="NGKIKVFKGPYDEWVEWNKRMNAQAKASSPKKKNEMPPEEPVKP
                     PPVQQPVNKEAKKQLQKQQRIVDQLDEKLKVLKEDSRMLELQLSAPEIYADKQKFMDA
                     EMAYKKADQALSEGRLAYDAALERLMELEEGQA"
BASE COUNT          699 a          700 c          655 g          744 t
ORIGIN      
        1 agaagaaatt ttgcagcagg tgaatttacg ctgaacagtc atggcgctgc gccaatatgc
       61 ccacaatcaa aagggtattc caatattcag gtatcccatc acccttttcc actggtcgca
      121 atacattgcg ctgatctcca gcgggcccag cgcgaaatta tacgtgaacg ttagcgcata
      181 gcccgataag aaatccgggc tgttgtaaaa ctgggcttta ctcacaaaat tattaaacag
      241 catattggcc cttcccgtca ggaagatatt gttgaagagc tgataacgga atccaagctg
      301 cgtggccgct acgctggccg aataaaatga tccttcccgg agacccgcaa aagtgatctg
      361 gttatgaaaa ctggaagtaa gcccgccgat cgaaaactca ttcatgatat tattattata
      421 ccggaaattg attccggatt gcagattaaa caggagggtg cttcttgaac ttaaaggcgt
      481 atagctttcg aaatgaaaga gcaaccgggg ataggggctg cgggaagcca ccgtatccat
      541 gtccaggtta ttgaaatgaa gctgtatatc cgggttctgc tgataaaccc aatctccttc
      601 cagttccgtt ttaacccctt ttcgcggata caccggttta tccagcgagt tgtgcttcag
      661 gtaaacataa ctcgtgagaa aatcattggt gcctttaaag acgagtgtgg aggagatgga
      721 gggtttgtag cggagccatt caaaccgcga tccggcggcg aaggtcaggt tccggtttgt
      781 ggagtatccg atccgggcgt ctgagacaaa ataattttca ttgtaaatcc cggcttcctt
      841 cgtatcgtta taagaggtta tattaaattg atcaaactgg gtggataatc cgaaagcaaa
      901 ttttcttcct ctcccgaaat actgcaggtg ctcgcccctt atccggaaat tttcgccgat
      961 gtcgatcgta gccatacttc tggaactcgg tgtgaaaaaa tcccgggtcg tcaaattcag
     1021 gatcgcgctt attccgctga actgattgta atgcagggcg accttaccaa aagagagcgg
     1081 gttctcggaa acatcaaaga tgatcttcga gctatgatct tcctgggggt ccaggaaata
     1141 tacaatccgg ttataatagc gggttcctgc ttcgcggcgg atcatctgcg ccagattttc
     1201 gggcgtatat ctccggtgcg tgagcaggcc catggtgtgt atgaaaaagt tctccgtggt
     1261 atgcttcagt ccgcgtaccg cataagaagt gatcaggacc gaatctgccc cgggcatttt
     1321 tcttttgggt tgcgggggtc cgtatatcgc gttcagtgaa tccgcgagcc gtttcagccg
     1381 gggatataat ttccttcctt cttcgatccc ggcttcgatg atctcatctg cctgcgaaaa
     1441 gctgcccata ttgaatttct ccaggggcgt ttgaacatat atatcacaaa gcggcacctc
     1501 cagtttcgta tcttctgctt cccggaaaaa ggccacctgc agcagtacct ggagggcatt
     1561 attcacttta ttggctgcca gcaaaccctg ggatacattg ctcccgatca ctatctgggc
     1621 ccccatgtcc cgcaggtcct taaccggaaa attccgcact acgccgccgt ccaccaatcg
     1681 ttttccatta taatcgaccg cagtaaacac ggaaggaatc gccatactgg cccgaatagc
     1741 cgaaaccagc tcgccgctgt ccagcaccac ggcttctccc gtggaaagat cggttgcaat
     1801 gcacttaaaa ggaatgggca gctgattaaa gtctttgatc tgatgaacag aaaacaaaag
     1861 ctcggaaaat ttcaaccaga gttcctgtcc ttccagcacg ccggtattca accggaaaaa
     1921 atggttcacc cagggaagtt ccaggtcata tttcccatat tcatcctttt cttccatgat
     1981 gatattacga agagaagact gattggagag catcaggtcc caatcgatat ttcctgccac
     2041 tttttctatc gaatcaccgg aatacccgga tgcatataag gatccgatga tgcttcccat
     2101 gctggtgccg gtaatatagt ccaccgataa tcctgccgaa tcaatggctt tcaggatgcc
     2161 gatatgcgcc agtccttttg cgcctccgcc actaagggca agaccgatct ttggtctggt
     2221 ggttaaatgc aagggtgtct gggcaaccgc atcagatagg agtgagcaga gataacagcc
     2281 aaaaagaaca cccgtccggc caaaagcacg aaaaaacggt aagcgattga tagttgataa
     2341 gctttatgaa taaaattagg aaaaccgccg gaggatacca tatctgcctt atgcctgccc
     2401 ttcttcgagc tccatcagcc tttccagggc ggcgtcataa gccagacggc cttccgacag
     2461 cgcctgatcc gcttttttgt atgccatctc cgcatccata aatttctgtt tgtcggcata
     2521 gatctcggga gcggaaagct gaagttccag catccggctg tcttctttca gaaccttcag
     2581 cttttcatcc agctgatcca cgatacgctg ctgtttctga agttgctttt ttgcttcctt
     2641 attgaccggt tgctgaaccg gcggcggctt gaccggttct tctggaggca tttcattttt
     2701 ctttttgggg gaactggctt tcgcctgcgc attcattctt ttattccatt ccacccattc
     2761 gtcgtatggc cccttgaata cctttatttt tccattgt
//
LOCUS       DASYCU010000549         3738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1276447, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000549 DASYCU010000000
VERSION     DASYCU010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276447"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VGM24_11095"
     CDS             complement(<1..589)
                     /locus_tag="VGM24_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4155969.1"
                     /translation="MIKGQGFLLISLLILFSSRLSPHTCFDFLNKTGSAGEIRSLAQE
                     NQAPQVHITRPASRSSYHRDARVPYSIRVSDAEDGESDYQEINPKEVLLKVKYLPDAG
                     KINAYLKQKDNRDSLDLQQMIRSNCFNCHAVKSRLIGPSFLEIFNHYPHTQSKMDSLV
                     RRISSGSSGIWGREVMPSHPELTKKEIQNMMNWIMS"
     gene            complement(614..2095)
                     /locus_tag="VGM24_11100"
     CDS             complement(614..2095)
                     /locus_tag="VGM24_11100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4155970.1"
                     /translation="MEDLKRRDFIKNTLMAGVGSSIGLPLIRPEEVAGKTNPESENNP
                     RKPAVAKKIIVAGAGISGLCCAYELMKSGHEVLVLEASGRHGGHVFTGRDGLSDGLYA
                     DYGADHITRPGYEFFFEYTKELDLPVLPYRQGNDSLQMIGGKFYSEEMLADPAILKKF
                     GFNEREVKELSLKPWWALQPLYLDSYIKKITDPYQPFGVGLDELDQLSLADLYKREGA
                     SPAALDHLGGKNTSALYKLWRLAVMGFRGIPLSEGETFRLKGGNQQITNAFAKHLGSR
                     VKLGSPILAINQTDKGVTVTYKEFGYPEEKQLSADFLVNCISLPVFRNILVTPALSAQ
                     KQYVIDHLGYTSHPHYIFEASSRFWLEDGFKSINMEFEHPDISAVWQTADEVDTGRVV
                     LVAFGPGGLSPQRVLAAFRKVYPGKRDSIVQALTKDWTKDNFAPTCEMVPFPVGEMHK
                     FWPELMKPDGRIYFAGTYADNLSRGMESCLRSAKRVANEIDHI"
     gene            2239..3021
                     /locus_tag="VGM24_11105"
     CDS             2239..3021
                     /locus_tag="VGM24_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY4155971.1"
                     /translation="MKKSILFICFLACAYPLLAQDSSTYQKETFIRNGDTLRYRILYP
                     ENAKKRKAYPLLVFLHGSGERGNDNTLQLAHGGDLFLKPDLRKQFPAVIIFPQCPARE
                     AWSKFPRKRDTSAAFNHLLNMDSLTRPEQLVKLLMDSLAEHKRIDKRRIYLGGLSLGG
                     FGTYDLVIHYPDYFAAAFPICGQANVPLYLRTASRVPVWIFHGGKDNVVNPQPDRDLI
                     KALQDAGVKTAKYTEYPEAGHNSWDSAFAEPDLLPWLFAQKK"
     gene            complement(3090..>3738)
                     /locus_tag="VGM24_11110"
     CDS             complement(3090..>3738)
                     /locus_tag="VGM24_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4155972.1"
                     /translation="YPQFRPRSGRIESVEFKINECLERSMNGNKIDSTSLEMRSMTAY
                     IKWVGKNVPGGTKPAGAGTEELLLLNRQADSLKGSRIYRLKCQSCHGTEGQGLSNADQ
                     TAYIYPPLWGEHSYNTSAGMYRITKLAGYIKYAMPLGTRYPLARLKDEQIWDVAAFIS
                     SKPRPVKSFKYDWPVLKTKPFDYPFGPYADSVPRQQHKYGPFTFMKRTGDSPAKK"
BASE COUNT          887 a          926 c          918 g         1007 t
ORIGIN      
        1 tgctcatgat ccagttcatc atgttttgaa tttctttttt cgttagctcg ggatggcttg
       61 gcatcacttc cctgccccag atcccggacg accctgagga gattcgccgg accagggaat
      121 ccatcttcga ttgggtatgc gggtaatgat tgaatatttc caggaaggaa gggccgatca
      181 gccggctttt tacagcatga caattgaaac aattggatct gatcatttgc tggagatcca
      241 gggaatcccg gttgtctttt tgtttcaggt aagcatttat cttacccgcg tccggcaggt
      301 atttgacctt cagcaacact tctttcggat ttatttcctg atagtcagac tctccatctt
      361 ccgcatcgga gacccggatg ctgtagggta cccgcgcatc ccggtggtaa gaactacggc
      421 ttgccggcct ggtaatatgg acctgcgggg cctgattttc ctgtgcaagg cttcggattt
      481 ctccggcgga tccagtttta ttgaggaagt caaagcaggt atggggtgaa agccgggagc
      541 tgaaaaggat cagcaggctg attaaaagaa atccctgtcc ttttatcatg atgctgcctg
      601 ttggtgactg aggttaaata tggtcaattt cattggcaac gcgctttgcc gagcgcaggc
      661 aggattccat tccccggctg agattgtcgg cgtaggttcc cgcgaaataa attcttccat
      721 ccggtttcat cagttcgggc cagaatttat gcatttcacc aaccggaaaa gggaccattt
      781 cacaggtcgg ggcaaaatta tcttttgtcc agtccttcgt caatgcctga acaatgctgt
      841 cccgcttgcc cggatatact ttccggaatg cagccagcac gcgttgcggg gagagaccgc
      901 ctggaccaaa tgcgaccagg accacgcggc cggtatctac ttcatccgcc gtctgccaga
      961 cggcggaaat atccggatgc tcaaattcca tattgattga tttgaagcca tcctccagcc
     1021 agaatctgga actggcctcg aatatataat gcggatggga agtatagcct aaatgatcga
     1081 tcacatattg tttttgagcc gaaagggcgg gcgttaccag aatgtttcta aagaccggta
     1141 atgaaatgca attcaccagg aaatcggcgc tcagctgctt ttcttcgggg tagccgaact
     1201 ctttgtaggt aacggtcacg cccttatcgg tctgattgat cgccagaatg ggactgccca
     1261 gttttacccg ggaaccgagg tgttttgcaa aagcattggt gatctgctgg ttgccgcctt
     1321 tcagccggaa ggtttctccc tccgataacg gaatgccgcg gaatcccatg acggccagtc
     1381 gccagagttt atataaggcc gatgtgtttt ttccgccaag atgatccagg gcggcagggg
     1441 atgctccttc tcttttatac aggtcggcga gggacagttg gtcgagttca tccagcccga
     1501 ccccgaaagg ctgatacgga tcggtaattt tttttatgta agaatccagg taaagcggtt
     1561 gcaatgccca ccagggcttg agagaaagtt cctttacctc ccgttcattg aaaccgaatt
     1621 ttttaagtat agccggatcc gccagcattt cttcagaata aaattttccg ccaatcatct
     1681 gtagactgtc attgccctgc cggtagggaa gcacgggaag gtccagttct tttgtatatt
     1741 caaaaaagaa ttcataaccc ggccgggtga tatgatcggc tccgtaatcc gcatataatc
     1801 catcggacaa gccgtcgcgt ccggtaaaga catggcctcc gtgtcttccg ctggcttcca
     1861 gaacaaggac ctcgtgcccg cttttcatca gttcataagc acagcacaat ccactgatgc
     1921 cggcgccggc aacaataatt ttctttgcaa ccgccggttt tcgcggatta ttttcagatt
     1981 ccggattcgt tttaccagcc acctcctccg gccggatcag gggcaacccg atcgagcttc
     2041 ccaccccggc catcaaggta ttcttaatga agtcccttct ttttagatct tccatccggc
     2101 ggaattgaaa tagttttgat taatcctgaa tgttaaggaa tgtcctcttt atattgcagt
     2161 ggttaaaaat aaaattttta aacggtactc caattatttt ttgcgttccc cttaatatta
     2221 atcaaacaag cggttgccat gaaaaagagt atattattta tttgctttct tgcctgcgcc
     2281 tatccgttgc tggcccagga cagcagtacc tatcagaaag aaacttttat ccgcaacgga
     2341 gacacgctgc gttaccggat cctttatccg gaaaacgcaa aaaagcggaa agcatatcca
     2401 ctgctggtct ttttgcatgg ttcgggagaa aggggaaatg acaacacatt gcagttggct
     2461 cacggaggcg atctgttcct gaagccggat ctgcgtaagc aatttccggc agtgatcatt
     2521 ttcccccagt gcccggcaag agaagcctgg tccaaatttc cccgcaaaag agatacttcc
     2581 gctgcattta accacctgct caatatggat agcctgacca gaccggaaca actggtcaag
     2641 cttttaatgg acagtctggc tgagcataaa agaattgata aaagaagaat ttacctgggc
     2701 ggattatccc tgggcggatt cggaacctat gatctggtta ttcattaccc ggattatttt
     2761 gctgcggcgt ttcccatttg cggtcaggcc aacgtaccat tgtatttgcg gacggcatcc
     2821 cgggttcccg tatggatttt tcatggggga aaggacaatg tggtcaatcc gcaacccgac
     2881 cgggatctga taaaagcatt acaggatgcg ggcgtaaaaa cggcaaaata taccgagtat
     2941 ccggaagccg gacacaatag ctgggacagc gcgtttgcgg agccggatct gctgccctgg
     3001 ttatttgcac agaaaaaatg acggatcgtt tccccgaagc cttttcattt taggatccgg
     3061 gaacaccgga acagccggcg gagcgtgtat catttctttg ctggcgaatc acctgttctc
     3121 ttcataaaag taaaaggtcc gtatttatgc tgctgccggg gaacactgtc cgcgtaaggt
     3181 ccgaagggat aatcaaaagg ctttgtcttc agcaccggcc agtcgtattt aaaagatttg
     3241 accgggcgcg gtttcgaact aatgaaggcg gccacatccc agatttgttc atcctttaac
     3301 cgggctagcg gatagcgggt acccaggggc atggcatatt ttatatatcc ggcaagcttg
     3361 gttatgcgat acatgcccgc gctggtatta taactgtgtt caccccataa aggcggatag
     3421 atataggcag tctgatccgc attggaaagg ccctggcctt ctgtgccatg gcatgactgg
     3481 cattttaacc ggtaaatgcg gctgcctttc aggctatccg cttgccggtt caggagaagc
     3541 aattcttccg ttccggcacc agccggttta gttccgccgg gcacgttctt ccccacccat
     3601 tttatatagg ctgtcatgct tctcatttcc aggctcgtac tgtctatctt attcccattc
     3661 atacttcttt ccaggcattc gttgattttg aattcaacgg attctatccg cccgcttctg
     3721 ggtctgaact ggggatac
//
LOCUS       DASYCU010000550         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4899435, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000550 DASYCU010000000
VERSION     DASYCU010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4899435"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..752
                     /locus_tag="VGM24_11115"
     CDS             207..752
                     /locus_tag="VGM24_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY4155973.1"
                     /translation="MKASEPNKMIWTIGHSTRPLAEFIDLLKSFDMEWVADIRSYPGS
                     RRFPQFNREALEVSLPQQQIRYIHLKNLGGRKKAGPDSKNTAWRNAAFRGYADYMETE
                     AFKEGMDELEKIASKYRTAFMCSEAVWWSCHRSMVSDYLKVRGWKVMHIMGIGKAEEH
                     PYTSAARIVNGELNYTLPELY"
     gene            843..>2617
                     /locus_tag="VGM24_11120"
     CDS             843..>2617
                     /locus_tag="VGM24_11120"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEY4155974.1"
                     /translation="MNRFVFFPLLVILFIGMAPSVQPKSGPENSWTAVSPEGSLKIIL
                     LKASGSLRYAVLINGDTAIRESALGLTFSDESFNNGLAFVRAEDSSIDDHYQMVTGKR
                     KKNEAKANQVTVTFKNKIDRRIQLVLRAYNDGVAFRYRFPDAKEKDTVTEESTAFSIP
                     LHGKAWLQSYSLPADWAPSYENMYSNGTPIGENARDSSGYSFAALFQSKNNWLLLTEA
                     GLDENFYGSHLQEHCENGIYRIARPQSGEARGLYSPFAITSGPFATPWRVIITGKSLK
                     TIFESNLVNHLSDPNRIGDVSWVKPGRSSWSWWSDHKSSRDFNSLKKYIDLSKEMGWE
                     YSLVDANWDLMQGGNIEQLANYAKTLGVRLSLWYNSAGPHSKITERPRDIMSDPQKRK
                     EELKKIRAWGIAAIKVDFFNSDKQELIKLYQDILKDAAAEHIMVLFHGCTLQRGWART
                     WPNLVTMEGVHGAEQYTWDKKFSAESPAQNIIYGLTRNVVGSMDYTPVTFSSYPCCRH
                     STTNAYELALSVLFESGIIHFADGAGNYEKTDANIHEFLRKVPSTWDDSRFIQGYPGK
                     ELVIARRSGDDWYVAAANGEDMEKP"
BASE COUNT          720 a          646 c          661 g          590 t
ORIGIN      
        1 acagtgaaca gctttttcaa tacgcgaagg atcagggctt tcatatatca tcaaatgata
       61 tgccctgcct ttaattccct ctcccggcca tctgtaaggc taattcataa tattattgtg
      121 gatttttatt cccggaatta acttttccct ttagggagcg gctatccggc gcggagtata
      181 tttgtgagca aacaaaccag gccggaatga aagcatccga accaaataaa atgatctgga
      241 ccatcggcca ttccacccgt ccgctggctg aatttatcga cctgctgaaa tcattcgaca
      301 tggaatgggt cgccgacatc aggtcctatc caggctcccg gcggtttccg caattcaaca
      361 gggaagcgct ggaagtttcc ctgcctcaac aacagatccg gtacattcat cttaaaaatc
      421 tgggtggaag aaaaaaggcc ggtcccgatt ccaagaacac ggcctggcga aatgcggcat
      481 tccgcggcta tgccgattac atggagacgg aagcttttaa agaaggaatg gatgaactgg
      541 aaaaaatcgc aagtaaatat agaactgcct ttatgtgctc cgaagccgtg tggtggagct
      601 gtcaccgttc aatggtttcc gattatctga aggtccgggg ctggaaagta atgcatatta
      661 tgggcatcgg aaaagcagaa gaacatccgt atacctcggc ggcgagaatt gtaaacgggg
      721 agctgaatta cacccttccg gaactctatt agtgcattgg ggactgcggc ctggtagcat
      781 agtgcaggac gcaaaagtct gtggattaac tcaaattcgt gcggaatagt tgaccattcc
      841 atatgaaccg atttgtattc ttcccactgc tggttatttt attcattgga atggcgccct
      901 ccgttcagcc caaaagcggt ccggagaaca gctggacggc cgtctcaccg gaagggtcct
      961 tgaaaatcat cttactgaag gcatccggga gcctgagata tgcggtactg atcaatggcg
     1021 acacggccat ccgggagtcg gcgctggggt taacattttc tgacgaatct ttcaataacg
     1081 gattagcctt tgtccgtgcc gaagacagtt ccatcgacga tcattaccag atggtaaccg
     1141 gcaagcggaa aaaaaacgag gccaaagcaa accaggtgac cgttaccttt aagaacaaaa
     1201 tagatcgccg gatccagctt gtccttcgcg cgtacaatga cggagtggcc ttccgttacc
     1261 gtttcccgga tgcaaaagaa aaagataccg ttacggaaga atccaccgcg ttctccatac
     1321 ctctccacgg taaggcctgg cttcaatcct acagtcttcc ggcagactgg gcaccttcct
     1381 acgagaatat gtacagcaac ggaaccccca ttggagaaaa tgcacgcgac agcagcggat
     1441 attcatttgc cgctttgttc cagagcaaaa ataactggct tttgcttacc gaagcaggcc
     1501 tggatgaaaa tttttatggc tcgcaccttc aggagcattg cgaaaacggt atttacagaa
     1561 tagccaggcc gcaatccgga gaagcccggg gactctacag cccttttgct ataacatccg
     1621 ggccatttgc aactccctgg cgggttatta ttacgggtaa atcactgaaa actattttcg
     1681 aatccaacct ggtgaatcat ttgtcggatc ccaaccggat cggcgatgtc agctgggtaa
     1741 agccgggaag aagctcctgg agctggtgga gcgatcataa gagcagccgg gattttaact
     1801 ccctcaaaaa atatattgac ctttccaaag aaatgggatg ggaatattca ctcgtggatg
     1861 ccaactggga tttgatgcag ggcggaaata ttgaacaact ggccaactat gcaaaaacac
     1921 tgggggtcag gctttccctc tggtacaatt ctgcaggccc gcacagcaag atcaccgaaa
     1981 ggccccggga tatcatgagc gatccgcaga agagaaaaga agaactgaaa aagatcaggg
     2041 cctggggcat cgcagccatc aaggttgatt ttttcaactc tgataagcag gaactgatca
     2101 aactatacca ggatatcctg aaagatgcag cggccgaaca catcatggtt ttatttcacg
     2161 gatgcacctt gcagcgcggc tgggcaagga cctggcccaa cctcgtgacc atggagggcg
     2221 tccatggcgc ggagcagtat acctgggaca aaaaattcag cgccgaaagt cctgcacaaa
     2281 atattatcta tggcctgacg agaaatgtgg tgggctcgat ggactatact ccggtcactt
     2341 tcagttccta cccctgttgc cggcactcca cgaccaatgc ctatgaactt gccctctccg
     2401 ttctgttcga gtccggaata attcattttg ccgacggagc cgggaactat gaaaagacgg
     2461 atgcaaatat tcacgagttc ctgcgcaaag tgcccagcac ctgggacgac agccggttca
     2521 tccagggata tccgggcaaa gaactcgtga tcgcgcgcag aagcggggac gattggtacg
     2581 tggcggccgc aaacggagaa gacatggaaa aacccat
//
LOCUS       DASYCU010000551         4618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3743030, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000551 DASYCU010000000
VERSION     DASYCU010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743030"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VGM24_11125"
     CDS             <1..853
                     /locus_tag="VGM24_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEY4155975.1"
                     /translation="RNALKSVPPQETDIKESETVYWKKDKIGLIEIPLKCRSVSYQGI
                     FDTRANISSITQTYAKKLGLKILDVSYEEGSGITGIKFKTGLGVADSLYFGNILVRNV
                     LFQVMPDSILYIAPIKFSLDIIIGFPVIEQLKEIHLYRDGRMLIPLTSSKSDLHNFAL
                     DGLDPVISLKTDQDTLCFHLDLGADETVLYAAYFEKFKQRILKEGKKRSARYGGAGGI
                     QEKEVYVLPALHLSLGNKNITLDSVDVFTKKIFPEERFYGNLGRDFTGRFGELVFNFE
                     YMYVKGF"
     gene            900..1268
                     /locus_tag="VGM24_11130"
     CDS             900..1268
                     /locus_tag="VGM24_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4155976.1"
                     /translation="MFADKNLKAFVPTAAPDKAKLFYRDTLGLKLLSQDQYALEFEAN
                     GVLLRVTIVRELKPQPFTVLGWDVDDISSTIKKLNLQGVHCEKYSFLEQDPLGIWTAP
                     GGARVAWFKDPDGNLLSITE"
     gene            complement(1273..2610)
                     /locus_tag="VGM24_11135"
     CDS             complement(1273..2610)
                     /locus_tag="VGM24_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4155977.1"
                     /translation="MKRRDFVKNISIAGAGIGLVSPYQLFSQKAPAKIKLGIIGVGLR
                     GQNHLHNALGRADVDVVAICDIDDRMLQSAREIISKSGKPMPKVFTGDPHAYRKLLEE
                     KDLQGILIATPWEWHAPMILDGLSAGIKYIATEVILGITLQDHWDVVKATEHYGANVM
                     MLENVCYRRDVMAILQMVRMGMFGELIHMQGGYQHDLRGVKLNDGKSAYGIGVEFGEK
                     GFSEAHWRTNHSVHRNGDLYPTHGIGPIAEMLNINYGNRFVSLSSFASKSRGLHNYIV
                     EHGGENHPNAKVEFKLGDVVTTQIKTANGETILLQHDTSLPRPYSLGFRVQGTKGIWM
                     DVNHSLYIEGVSAKPDEWEEAKPYLEKYDHPLWKQYGSEAEGAGHGGMDFFVLQAFVE
                     SIKQQTPTPIDVYDSASWSSITALSELSIQANQTVEFPDFTGGQWMYRKNRFQ"
     gene            complement(2647..4029)
                     /locus_tag="VGM24_11140"
     CDS             complement(2647..4029)
                     /locus_tag="VGM24_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY4155978.1"
                     /translation="MPIPEFKSVFPWNQELIATYPLLKDPELEERIQWAALAFSTWKN
                     KSFSQRGDILKKVAVLMRRDQESLATLISREMGKVIPESRAEIEKCAWVCDYYAENGG
                     AFLQDEIREAGYTKSFISYQPLGAVLAIMPWNFPFWQVFRFAAPALMAGNVGLLKHAP
                     NVCGCATAIERLFQEAGADRGVFSSLIVDVPAVEKIISSGIVQAVTLTGSERAGSSAA
                     ALAGKHIRKSVMELGGSDALVVLADADIKSAAATAVQSRMQNAGQSCIAAKRFIVVKS
                     VEAGFLGEITAGIKKLIQGNPLEPGITTGPLARPDLADHIQKQMQESIQKGAKLEFGG
                     GINGCNVIPSLLTGVQKGMPAFDEETFGPMAVVITARDEEEAIRLANDSAYGLGGSIW
                     TRDLDRGIALAKKMESGAVFINGLVKSDPRLPFGGIKQSGYGRELGIHGIREFVNIKA
                     IAGQSAIYLQ"
     gene            complement(4091..>4618)
                     /locus_tag="VGM24_11145"
     CDS             complement(4091..>4618)
                     /locus_tag="VGM24_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4155979.1"
                     /translation="TEQLLPGQLPDIVLLDIHMPVMDGYATAAWIRANHPEIRVMALS
                     TMDSDMAIIKMIHQGARGYILKDADPKELQLAFNEIVDKGYYYNEILTRKVMQSIHQG
                     NTESGNGFPKLTDRELEFLQHACSEKNYQQIAREMFLSERTVDGYRESLFKKCNVSNR
                     VGLVLYAIKNQLVKL"
BASE COUNT         1058 a         1168 c         1164 g         1228 t
ORIGIN      
        1 ccggaatgcc ctgaaatccg ttccgccgca ggaaacggac atcaaggaaa gcgaaacggt
       61 ctattggaag aaagataaga tcgggttgat tgagatcccg ctgaaatgca gatcggtaag
      121 ctatcagggc atattcgata cccgggcgaa tatttcctcg atcacccaaa cctatgcgaa
      181 gaaactcgga ctaaaaatac tggatgtttc ttatgaagag ggaagtggta ttaccggtat
      241 aaaattcaaa accggtctcg gggtagcgga cagtttatat ttcgggaata tattagtccg
      301 gaatgtgctg ttccaggtga tgcccgattc cattctttat attgccccga taaaattttc
      361 gcttgacatc attatcggat tccccgtcat tgaacaatta aaagaaattc atctttaccg
      421 ggacggccgg atgctgatcc ccctgacctc atcgaaaagc gatctgcata attttgcact
      481 ggacggcctc gatcccgtca tttcattaaa aacggaccag gatacgttat gctttcatct
      541 ggacctggga gccgatgaga ccgtccttta tgctgcctat tttgagaaat tcaagcagcg
      601 tattctgaag gaaggtaaaa aaagatcggc ccggtacggc ggtgcgggtg gtatccagga
      661 aaaagaagtg tatgttcttc cggcgcttca cctttcgctg ggaaacaaga acatcacgct
      721 ggacagcgtg gatgtattca ccaaaaagat ttttccggaa gaacgcttct atggaaacct
      781 gggccgcgat tttacgggcc gcttcggtga gctcgtcttt aattttgaat atatgtacgt
      841 taaagggttt tagtttatta cttttcccgt tttctcctgt tcattttaaa atcttcccca
      901 tgtttgcaga caaaaatctt aaagcttttg ttcccactgc ggccccggac aaggcaaaat
      961 tattttacag agatacttta ggattgaaat tattatcgca ggaccagtac gccttggaat
     1021 ttgaggcgaa cggggtatta ctgagggtga cgatcgtccg ggaattgaaa cctcagccat
     1081 ttaccgtttt gggctgggat gtggacgaca tctcttctac gataaaaaag cttaacctgc
     1141 agggtgtcca ctgtgaaaaa tacagttttt tggaacagga tccattggga atctggactg
     1201 ccccgggagg tgccagagta gcctggttca aggacccgga cggaaatctg ctctctatta
     1261 cggaatgacc gcttattgaa accggttctt ccggtacatc cactgcccgc cggtaaaatc
     1321 ggggaactcc acggtctgat tggcctgtat gctcaattcg cttagtgcgg tgatagagct
     1381 ccaggaagcc gaatcatata catcgatggg tgttggggtt tgctgcttaa tggattccac
     1441 aaaagcctgg agaacgaaga aatccattcc gccatggccg gctccttcgg cttcgctgcc
     1501 atattgtttc cacagggggt gatcatattt ctccaggtaa ggttttgctt cttcccattc
     1561 atcgggtttg gcgctgacgc cttcgatgta aagggaatgg ttcacgtcca tccagatccc
     1621 tttggttccc tgaacgcgga agccaagaga ataggggcgg ggcaggctgg tgtcgtgctg
     1681 taataaaatg gtttctccgt tggctgtttt gatttgcgtc gtgaccacgt ctcccagctt
     1741 aaattccact ttggcattgg gatggttctc tccgccgtgt tcaacaatat aattgtgcag
     1801 gcccctgctt ttggaggcga aggaagaaag ggaaacaaac cggtttccgt aattgatatt
     1861 taacatttcc gcgatcgggc ctatgccatg tgtcggatag aggtcaccgt tgcggtggac
     1921 cgaatgattg gtccgccagt gcgcttcgga aaaccccttc tctccaaatt caaccccgat
     1981 cccgtaagct gatttaccgt cattcagttt cactccgcgg aggtcgtgct ggtaaccgcc
     2041 ctgcatatgg atcaattcgc cgaacattcc cattctaacc atttgcagta tcgccatcac
     2101 atccctgcgg taacagacat tctccagcat cattacgttg gctccataat gctccgtagc
     2161 ttttaccacg tcccaatgat cctgcagcgt aatgccgagg atcacttcgg tggcaatata
     2221 ttttatgccg gcggataatc cgtcgaggat catgggggca tgccattccc agggagtggc
     2281 gatgaggatt ccctgcaggt ctttttcttc cagcagtttc cggtaggcgt gcggatctcc
     2341 cgtaaatact ttcggcatgg gtttgccgga tttgctgatg atctccctgg ccgactgcag
     2401 catacggtcg tcaatatcgc aaatggccac cacgtctacg tctgctcttc ccagggcatt
     2461 gtgcagatga ttctggcctc ggagtcccac cccgataatg ccaagcttta ttttcgcggg
     2521 tgctttttga gaaaacagct ggtaggggct gaccaggccg atgccggctc cggcaatgga
     2581 aatatttttt acgaaatctc ttcttttcat gagttcaatt ttagattgat ttgaaatttc
     2641 cggaaactat tgtaaatata tagcggattg accggcaatc gccttgatat tgacgaattc
     2701 gcgtataccg tgtatgccca gttccctccc atagcccgat tgttttatcc ccccgaatgg
     2761 caggcgggga tcggatttta ccaggccatt gataaagacc gcacccgact ccattttttt
     2821 cgcgagtgcg attcctctat ccagatcacg ggtccagatg cttcctccca agccataagc
     2881 agaatcgttg gcgagccgga tggcctcttc ttcatcccgg gcggtgatca ccacagccat
     2941 cggaccgaat gtttcttcat caaaagccgg cattcccttt tgcacgcccg tgagcaggga
     3001 agggatcaca ttgcatccgt ttatcccgcc gccgaattca agctttgcgc ctttttggat
     3061 ggactcctgc atttgttttt gaatatggtc tgccaggtcc ggccgggcaa gcggccccgt
     3121 ggtaatgccg ggctccagcg gatttccctg tatgagtttt tttattccgg cagttatttc
     3181 cccgaggaag ccggcctcca ccgatttaac cacgataaag cgcttggctg cgatgcagga
     3241 ctgacccgca ttttgcatac gcgattgaac cgccgtggcg gctgcgcttt ttatatcggc
     3301 atctgcgagt acgaccaggg catccgatcc cccgagttcc ataacggact tgcggatatg
     3361 tttcccggcg agggcagcag ccgaactgcc cgctctttca ctccccgtca gcgtcacggc
     3421 ctgcacgatc ccggaagaaa ttattttttc cactgccgga acgtctacga tgaggctgct
     3481 gaacacgccc cggtcagcgc cggcttcctg aaaaagtctt tcaatagcag tggcacatcc
     3541 gcaaacattc ggcgcatgtt ttaagaggcc tacgtttccg gccatcaggg ctggtgctgc
     3601 aaaccggaaa acctgccaga acggaaaatt ccagggcatg atcgcgagta ccgcgcccag
     3661 gggctgataa gaaataaaac tctttgtgta tcccgcttcc cggatttcat cctggaggaa
     3721 agcgcctccg ttttcggcgt agtaatcgca gacccaggcg catttttcaa tctcggcgcg
     3781 ggattcgggg atcacttttc ccatctcgcg gctgatgagc gttgccaatg attcctggtc
     3841 cctgcgcatc agcacggcca cttttttcag aatatcaccc cgttgggaaa aggatttatt
     3901 tttccaggtg gaaaatgcaa gagcggccca ttgtatgcgt tcctccagtt caggatcttt
     3961 cagcagtgga taagtggcga tcagttcctg gttccatggg aaaacagatt tgaattcggg
     4021 aatcggcatg cagcgaattt actgaaaaat gccgaaggca gaatggcagg cgtccgggca
     4081 acaccgctga tcagagtttc accagttgat tcttgatcgc ataaaggaca agccctacgc
     4141 ggttactgac attgcatttt ttgaagaggg attcccggta gccgtccacc gtacgctcgc
     4201 tgagaaacat ttcccgcgcg atctgctggt aattcttttc actgcaggca tgctgcaaaa
     4261 actccagttc gcggtccgtg agcttgggaa atccgttccc ggattcggtg tttccctggt
     4321 gaatggactg cattactttg cgggtgagga tctcgttgta ataatatcct ttgtccacga
     4381 tctcattaaa tgccagctga agttctttgg gatcggcgtc ttttaaaatg tatccccggg
     4441 cgccctggtg aatcatcttg ataatggcca tgtccgaatc catggtgctg agggccatta
     4501 cccggatttc cggatgattg gcgcggatcc aggcggcggt tgcatatccg tccattaccg
     4561 gcatgtgaat atccaggagc acgatatcgg gcagctgacc gggaagcagt tgctccgt
//
LOCUS       DASYCU010000552         5460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3454111, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000552 DASYCU010000000
VERSION     DASYCU010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454111"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1071
                     /locus_tag="VGM24_11150"
     CDS             130..1071
                     /locus_tag="VGM24_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY4155980.1"
                     /translation="MHQPPDILSLAYKQPVMYEQLHLLQEKERQIPGSVQYAIQRYRK
                     NSQWSMDDLGMMTYHYEKTRPAENYFELRFCIAGNIYCKQKDTECDSCRLNVSRYCQE
                     RIETVDVLSFQFSAVHLSQFVKPQQVAGTLADNVLHFKHPSSFSKILSLCSRIRLVLE
                     ALLNHTYTDSLENIFINAQTQMLLLYSLECMLGEKEVDAFQCKFLANENDREKITLAR
                     KILLEHIGEPLTIRELSRKVAINECYLKKGFKELFGTTIFDFYQSQRMEHARYLLYEK
                     GLSVTEVSIMLGYSSISHFSTAFKKHTGLKPCELLLH"
     gene            complement(1087..2385)
                     /gene="hemL"
                     /locus_tag="VGM24_11155"
     CDS             complement(1087..2385)
                     /gene="hemL"
                     /locus_tag="VGM24_11155"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY4155981.1"
                     /translation="MNIPNSIRLFDRAQETIPGGVNSPVRAFRSVGGTPVFINKAKGA
                     YLYDADGNKYIDYIASWGPMILGHAYEPVVKAIQEKVTGSTSFGAPTELEVEMAELIC
                     SMVPNVDLIRMVSSGTEACMSAIRLARGFTGKNKFIKFEGHYHGHADSFLVKAGSGLA
                     TFDIQTVPGVTAGVAQDTLTCAFNDPEAVRQLVKQHRGAIAAIIVEPVAGNMGCVLPE
                     KGFLEELRSICDAENMLLIFDEVMTGFRLAPGGAQEKLGIRADLVTYGKVIGGGLPVG
                     AFGGRREIMEQIAPLGKVYQAGTLSGNPVALIAGLTTLKMLKENPQIYTGLEKKTIYL
                     KEGLQKALEKKGLPVVINHFGSMISVFFSGRPVRDFASAAAGDTERFKSLFHSLLNDG
                     IYLPPSAFESWFLNNALTMRDLDETIDAAGEWKIEGREPH"
     gene            complement(2478..3455)
                     /gene="hemB"
                     /locus_tag="VGM24_11160"
     CDS             complement(2478..3455)
                     /gene="hemB"
                     /locus_tag="VGM24_11160"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY4155982.1"
                     /translation="MYLQKRSRILRNSPTIRSLVSETSLSANDFIVPLFIDEGRQVRT
                     EIASMPGYYRNSLDLTVNEVKELYRMGLKSILIFIKCKDELKDNTGKEAWNRDGLMQR
                     SIRAIREAVPEMLIMTDVALDPYSSYGHDGIVREGEIVNDETVEALVKMSLSHAEAGA
                     DFVAPSDMMDGRIGAIRSAFEKNGFIRTGIMAYSAKYASCFYGPFRDALGSAPGFGDK
                     KTYQMDYANRGEAVKEALQDVEEGADIVMVKPALSYLDIIREVKNAVRVPVSAYNISG
                     EYAMIKAAAKMGWIDENKAILETLTSMKRAGADLIATYFAKEATRLLDL"
     gene            complement(3490..4443)
                     /gene="hemF"
                     /locus_tag="VGM24_11165"
     CDS             complement(3490..4443)
                     /gene="hemF"
                     /locus_tag="VGM24_11165"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY4155983.1"
                     /translation="MMNASRLYKTATKEFRGRWIEYVYGLQDTICAALEKVDGKAVFL
                     TDEWERAGGKGGGGRSRVLQNGNVFEKGGVNTSVVYGHVTEALRKQLKIEGDTWFACG
                     LSLVIHPLNPYVPTVHANWRFFELYDSEGCVADRWFGGGTDLTPYYLFEEDAKHFHQT
                     LKNGLDSFDRNLYPLYKSNCDEYFSNSHRNNETRGIGGIFYDYLRPADGADAERLLNF
                     QKQNGDSFLDAYLPIVQKRKGLPYTEKEISWQEIRRGRYVEFNLIHDRGTGFGLRTNG
                     RTESILMSLPPRARWIYNHLPDPGTPEDELLQVCLKPRDWV"
     gene            complement(4445..>5460)
                     /gene="hemE"
                     /locus_tag="VGM24_11170"
     CDS             complement(4445..>5460)
                     /gene="hemE"
                     /locus_tag="VGM24_11170"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY4155984.1"
                     /translation="EITERAPVWMMRQAGRYLPDYRKLRDKYSFFERVQTPALATEIT
                     LQPVDQIGVDAAILFSDILVVLQAMGLEVQLIENTGPVLPAPVRTESDAQRVFVPDVE
                     EALGYVFESIRLTKQELNGRVPLIGFAGAPWTLLCYMIDGKGSKTFDLTKSFCYTKPE
                     LAHRLLQMLTDTTIAYLRQQVRAGTDVVQLFDSWGGLLGPDDFEIFSLQYMRQIIRAL
                     KAESPTILFAKGAWHSLAEMAATGASGIGLDWCIRPETARELAGRQVTLQGNFEPARL
                     LTPIPELKKQVTRMLNQFGPQRHIANLGHGILPDVPVDHARAFVDTVKNHVYKPAIAE
                     AKS"
BASE COUNT         1228 a         1471 c         1328 g         1433 t
ORIGIN      
        1 gcctgttttt tgaactattt tttacgttct ttcgcgtgta aaaatgacag ttttatgatg
       61 gttctaaccg ttgttccccc ttttgccgta ttgccggaag ctatttgcgt tgtaaattcg
      121 tgcccgatta tgcatcagcc ccctgatata ctttccctgg cctataagca gccggttatg
      181 tacgaacagc tccatctcct acaggagaag gagcgtcaga ttcccggctc cgttcagtat
      241 gcgatccagc gctaccgtaa gaattcgcaa tggagcatgg atgatctcgg gatgatgacc
      301 taccattatg agaaaaccag gccggcagaa aattatttcg aactgcggtt ctgtattgcc
      361 gggaatattt actgtaaaca aaaagacacg gaatgcgatt cctgccggtt gaatgtttcc
      421 cgctattgcc aggagcgcat tgaaacggta gatgtgttga gttttcagtt ctcggcggtt
      481 catctttcgc agtttgtcaa accccagcag gtggcaggca cccttgcgga taatgtgctt
      541 cactttaaac atccctcctc cttttccaag attttgtccc tttgcagccg tatccgcctg
      601 gtgctggagg ccttattgaa tcatacctat accgacagtc tcgagaatat ctttatcaat
      661 gcgcaaaccc agatgcttct gctttacagc ctggaatgca tgctgggcga aaaagaagtg
      721 gatgcttttc aatgtaagtt cctggccaat gaaaatgacc gggaaaagat caccctggct
      781 cggaagatcc tgctggaaca tatcggcgaa cccctgacca tccgggagct cagccggaaa
      841 gtggccatca acgagtgtta tttgaaaaag ggatttaagg aactcttcgg caccaccatt
      901 tttgatttct accagagcca gcggatggaa catgcccgct atcttttata tgagaaaggg
      961 ctgagcgtta cggaagtttc tattatgctc ggttattctt ccatttccca tttctctaca
     1021 gcgttcaaaa agcatacggg actgaagccc tgcgaattac tattacatta gccagaaccg
     1081 ggaagatcaa tgcggttccc ggccctcaat tttccactct ccggccgcat cgatcgtttc
     1141 atccagatcg cgcatcgtga gcgcattgtt caaaaaccag ctttcaaagg ccgaaggggg
     1201 aagatagatt ccatcattca gcagggagtg gaataagctt ttaaagcgtt ccgtgtcgcc
     1261 tgcggcagcg gaagcgaaat ctcttaccgg ccggccgctg aagaatacac tgatcatgga
     1321 accaaaatga ttgatcacca ccgggagacc ttttttctct aatgcttttt gcagtccttc
     1381 tttcaggtag atcgtctttt tctccaggcc ggtatagatc tgcgggtttt ctttaagcat
     1441 tttcaaggtg gtaagaccgg cgatcagggc aacgggattt ccactgaggg tgcctgcctg
     1501 atagaccttt ccgagcgggg caatctgttc catgatttcc ctccggcctc cgaaagcacc
     1561 tacggggagt cctccgccaa tcacttttcc ataagtgacc aggtctgccc ggatgccgag
     1621 cttttcctgg gcgcccccgg gagcgagccg gaatccggtc atcacttcat caaatatcaa
     1681 tagcatgttt tccgcatcgc atatggatct caattcctcc agaaatcctt tttccggaag
     1741 cacgcagccc atattgccgg ctaccggttc aacaatgatg gcggcgatgg ccccgcggtg
     1801 ttgcttaacc agctgccgca ccgcttccgg gtcattgaaa gcgcaggtga gcgtgtcctg
     1861 cgccacgccg gcggttactc cgggtaccgt ctgaatatca aaagtagcca ggccgctgcc
     1921 ggctttcacg aggaaagaat cggcgtgccc gtgataatga ccctcaaatt tgatgaattt
     1981 attttttccg gtaaagcccc tggccagccg gatcgcactc atgcaggctt ccgttccgct
     2041 gctcaccatg cggatgagat ccacattggg gaccatgctg cagatcagtt ccgccatttc
     2101 cacttcgagt tctgtgggcg ccccgaaaga ggtggaaccc gttacttttt cctgtatcgc
     2161 ttttaccacg ggttcataag catggcccag gatcatgggt ccccaggaag cgatataatc
     2221 aatgtattta ttcccgtccg cgtcatagag gtatgctcct tttgccttgt taatgaaaac
     2281 gggggtgccg cccacgctcc ggaatgcgcg gacgggagaa tttacgccgc cgggaatagt
     2341 ttcctgcgcc cggtcaaata gtctgatact gttggggata ttcatgattt ctgtgtctgg
     2401 ccggaatcat tctgttattt tgtgttgaag atccggatga tgaaaacctg tgtgagggat
     2461 gaatggaatt gccgggatta tagatccagc agccgcgtgg cctcctttgc gaaataggtg
     2521 gcgatcagat ccgcaccggc cctcttcatg gaagtgaggg tttccaggat cgccttgttt
     2581 tcatcgatcc atcccatttt cgcagcggcc ttgatcatcg catattcccc gctgatattg
     2641 taggcactaa cgggtacccg cactgcattt tttacttccc ggatgatgtc gagataggag
     2701 agagcgggtt ttaccattac gatatccgct ccttcttcca catcctgcag ggcttcttta
     2761 accgcctcgc cgcgattggc ataatccatc tggtaggttt tcttgtcccc gaatccgggc
     2821 gcggatccca gggcatcccg gaaaggcccg taaaaacagg aggcatattt ggcgctgtag
     2881 gccatgatac cggtcctgat gaatccgttt ttttcaaaag cggaccggat ggcgccgatg
     2941 cggccgtcca tcatgtcgct gggcgcaaca aagtctgccc cggcctccgc atggctcagg
     3001 ctcatcttca ccagcgcttc cacggtttca tcatttacga tctcgccctc cctcacaatt
     3061 ccgtcatgac cataagatga atacggatcg agtgccacat cagtcatgat cagcatttcc
     3121 ggaacggctt cccggatcgc gcgtatactg cgttgcataa gcccgtccct gttccaggcc
     3181 tcttttccgg tattgtcttt caattcatct ttgcatttaa tgaatattaa aatgcttttt
     3241 aatcccatcc ggtataattc ctttacttca tttaccgtca gatccaggct gttgcggtag
     3301 tatccgggca tggaggcgat ttccgtccgc acctgccgcc cttcatcgat gaacaggggc
     3361 acgataaaat catttgccga cagggaggtt tctgaaacca ggctccgtat ggtgggactg
     3421 tttcttaaaa tcctgcttct tttttgtaaa tacataatcc ggtttttaat tcattgagaa
     3481 tggagatttc taaacccagt cccgcggttt caaacagacc tgcagcaatt catcttcggg
     3541 agttcccgga tccggcaggt gattataaat ccagcgcgcc cggggcggga gactcatcaa
     3601 tatactttcc gtccgtccgt tcgttcttaa tccgaacccg gtgccgcggt cgtggatcaa
     3661 attgaattcc acgtatctgc ctctcctgat ctcctgccag cttatttctt tttcagtgta
     3721 gggaagtcct ttccgttttt ggacaatggg aagataagca tccaaaaaag aatctccgtt
     3781 ttgcttttgg aagtttaata atctctccgc gtccgccccg tcggccggcc gcaggtaatc
     3841 atagaaaatt ccaccgatac cccgggtctc gttattccgg tgggaattgg aaaaatattc
     3901 gtcgcaattg cttttatata acggatatag atttctgtca aaggaatcca gcccgttctt
     3961 cagtgtctgg tgaaaatgtt ttgcatcttc ttcaaacagg taataaggag taaggtccgt
     4021 tccgccgcca aaccacctgt ccgccacaca accttccgaa tcatataact caaaaaaccg
     4081 ccagttggcg tgtacggttg gcacataggg attcaatggg tgaatgacca gtgaaaggcc
     4141 gcaggcgaac caggtatccc cttcaatctt cagctgcttg cgcagggctt cggtcacgtg
     4201 gccataaacg acggatgtgt ttaccccgcc cttttcaaac acatttccat tctgcaaaac
     4261 ccttgaccgc ccgccgcctc cttttccgcc ggccctttcc cattcgtccg tcaggaaaac
     4321 ggctttgcca tccacttttt ccagggcggc acaaatggta tcctggagcc cgtagacata
     4381 ttcaatccac cggccccgga attcttttgt tgcggtttta tataggcggg acgcgttcat
     4441 cattttaact tttggcctcc gcgatggcgg gtttatacac gtgatttttt actgtatcca
     4501 caaaggcccg cgcatgatcc acgggcacat ccggcagaat gccgtgaccg aggttggcga
     4561 tgtgccgctg cggtccgaat tgattcagca tccgggtcac ctgttttttt aattccggga
     4621 tgggcgtgag taagcgcgcc ggctcaaaat tcccctgcag ggtgacctgc cggcccgcaa
     4681 gttcccgcgc cgtctccggg cggatgcacc agtccagccc aatgccggac gcacccgtag
     4741 ctgccatttc cgcaagggaa tgccaggctc ctttggcaaa aagaatagtc ggcgattccg
     4801 ctttcaatgc ccggatgatc tggcgcatat actgaagcga gaaaatttca aaatcgtccg
     4861 gaccgagcaa acctccccaa ctgtcgaaca gctgaaccac atccgtaccg gccctgacct
     4921 gctgccgcag ataggcgatc gtggtgtcgg tgagcatttg cagtaagcgg tgtgcgagtt
     4981 ccggtttggt ataacaaaag gattttgtca gatcaaatgt tttggatcct tttccgtcta
     5041 tcatgtaaca cagcagggtc cagggagcac cggcaaatcc gataaggggt acccggccgt
     5101 tcagttcctg tttggttaag cggatcgatt caaaaacata tcccagcgct tcctccacat
     5161 ccggaacaaa aacccgttgg gcatctgatt ccgttctgac cggagccggc aaaacagggc
     5221 ctgtattctc gatgagttgc acttccagtc ccatggcctg aagaacaaca aggatatcag
     5281 aaaacaaaat ggcagcatcc actccgatct ggtccacggg ctggagggtg atctcggttg
     5341 ccagggccgg cgtttgcacc cgttcaaaaa aagaatattt atcccggagt ttgcggtagt
     5401 ccggcaggta acggcccgcc tggcgcatca tccaaaccgg ggcgcgttcc gtgatttctc
//
LOCUS       DASYCU010000553         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3017977, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000553 DASYCU010000000
VERSION     DASYCU010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017977"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VGM24_11175"
     CDS             complement(<1..1033)
                     /locus_tag="VGM24_11175"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HEY4155985.1"
                     /translation="MDKQQKRRAFLKTLTWGGLGAAAPIQIVKAERSLPAAEKMNPPG
                     GETAVQRKYNGLYTGDHLNRIAFPIGGLGAGMFCLEGTGAISHVSVRNKPDVFNEPAM
                     FAAICIKGLKNCAKVLEGQVPEWKYFGRRGSGNGLAGSTLGLPRFKTADCLTRFPYST
                     INLRDKELPLQVRITGWSPFIPTDEDNSGLPAGALEYRFVNTGTKKLEAVFSYNSKNF
                     LAGDEQSGNGIEKIPNGFVLTQQGTKDNPSLQSSFAIFSNEEHTVVDHCWFRGGWWDP
                     LTMAWNNIRDARVQAVDPVEKNAAGASLFIPFSLAPGSSRTIRVMMAWYVPETNLHSG
                     TVMTEEKKNC"
     gene            complement(1045..2409)
                     /gene="fucP"
                     /locus_tag="VGM24_11180"
     CDS             complement(1045..2409)
                     /gene="fucP"
                     /locus_tag="VGM24_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEY4155986.1"
                     /translation="MNLKQGNKIPGLLRAIDGTSYLRPFILITSLFLLWGFAHGLLDV
                     LDKHFQDTLHVSKAESGFVQFSLYIGYLVMAYPAGLFMKRYGYKKGIILGLSLFALGA
                     FLFFPIVKMHIQEKDALFISFLIALFIIACGLCCLETAANPYTTVLGPPPGAARRINI
                     AQSFNGLGWILGPLLGGFFIFGQDQPGNTGSSDSLLKPYMLVGTIVVGVAIIFAFTRL
                     PAIQEENEEGIKADAPTRELFRHPAFVLAVVAQFLYVAAQTGVNSFFINYVTETLTGV
                     QATIAGMMQHLSRFGQFFMPKNAEQAASLILAFGGMGSFWIGRLVGAYLMKFVRPQRL
                     LAIYAVINTILTAIVVMRFGWFSVIALFSTYFFMSLMFPTIFALGLRGLGPLTKKAAS
                     FLVMAVAGGAFCPPLMGAIADHFGMSIAFLIPMFCFAFIAWYAVTRKRPAGEAVNPAV
                     LMHH"
     gene            complement(2554..>4049)
                     /locus_tag="VGM24_11185"
     CDS             complement(2554..>4049)
                     /locus_tag="VGM24_11185"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEY4155987.1"
                     /translation="GSETMDLNPWIPKAIWGFNGTERPGAVYLAATLAAHNQMGLPAF
                     GIYGKDVQDLGDEHIPEDVQERLLGFARAGLAVAIMRGKSYLAVGSVCMGIAGSMVNP
                     DLFREYFGMRNEYVDSTELLRRIEKKIYSEAEFEKAISWVKANCKEGEDFNMASKQFS
                     REQKDKDWAFVVKMTLIIRDLMIGNPLLKERGFGEESQGHNALVSGFQGQRQWTDFLP
                     NGDFAEAILNSSFDWNGIRSPVIVATENDSLNGISMLLNYLLTNTAQIFADVRTYWSP
                     EAVKRVSGWSPAGAAANGFIHLINSGACTLDGTGRQSRNGKPAMKPFWEITPDEAEAC
                     LEATTWYPANNGYFRGGGYSSKFVSRGGMPVTMCRLNLVRGIGPVLQIAEGLTIDLPE
                     EVHRTLDERTDRTWPTTWFVPRLTGKGYFKDVYSVMANWGANHGAISYGHIGRDLISM
                     ASMLRIPVCMHNVPEEQIFRPAAWSAFGSDPEGSDYRACAAYGPLYS"
BASE COUNT          995 a         1103 c          993 g          958 t
ORIGIN      
        1 cacagttttt cttttcctct gtcataaccg ttccgctatg caggttcgtt tccggcacat
       61 accaggccat cattacccgg atggttctgg aagatccggg tgctaaggaa aagggaatga
      121 aaagagaggc gcccgccgca tttttttcaa ccggatcaac ggcctgcacg cgcgcatccc
      181 gtatattatt ccaggccatg gtcagcgggt cccaccagcc gccccggaac cagcaatgat
      241 caaccacggt gtgctcctca ttgctaaaaa tagcaaaact ggactgcagg gaaggattgt
      301 ctttggttcc ctgttgggta agtacaaaac cattggggat tttctcaatc ccatttccgg
      361 actgttcgtc cccggccaga aaattttttg aattgtacga aaagaccgcc tcgagttttt
      421 ttgtgccggt gttgacgaac cggtattcca aagccccggc aggcaacccg gaattgtctt
      481 cgtcggttgg gatgaaagga ctccaacccg tgatgcgcac ctgcagcggt agctctttat
      541 cacgcagatt gatggtgctg taaggaaacc gggtaaggca atcggctgtt ttaaagcggg
      601 gaagtcccag cgtggaaccg gccaggccat ttccactacc ccgccttcca aaatatttcc
      661 attccggcac ctgcccctcc agtactttag cgcaattctt aagccctttt atgcaaatgg
      721 cggcaaacat cgccggttca ttaaaaacgt ccggcttatt ccggacactc acatgggata
      781 tagcccccgt gccttccagg cagaacatac cggcccccaa tccgccgatc ggaaatgcga
      841 tgcgattcag gtgatctcct gtatataatc cgttgtattt tcgctgaaca gcggtttccc
      901 cgccgggagg attcattttt tctgcggccg gcaagcttct ttcggccttt actatttgaa
      961 tcggcgcagc agcgcccaaa cctccccagg tcagcgtttt taaaaaagcc cggcgctttt
     1021 gttgcttatc catctgtatt caatttaatg atgcatcaga actgccggat tcaccgcctc
     1081 acctgccggt ctttttctgg ttactgcgta ccaggcgata aaggcaaagc agaacatggg
     1141 aatgagaaaa gcaatggaca ttccaaaatg atcggcaatc gctcccatca gcggggggca
     1201 aaatgcgcct cctgccaccg ccatgaccag gaaagaagcc gcttttttgg taaggggtcc
     1261 gaggcccctc agtccaaggg caaaaatagt cgggaacatc agcgacataa agaaataagt
     1321 ggagaacagg gcaattacac tgaaccatcc gaagcgcatg acgacgatcg cggtcagtat
     1381 cgtattgatc acggcataga tcgccagcaa acgctgcggc cgcacgaatt tcatcaggta
     1441 agcccccacc aaacggccga tccagaatga tcccattccc ccaaacgcaa gaatcaggga
     1501 cgccgcctgc tcggcattct tcggcataaa gaattgtcca aaccggctca gatgctgcat
     1561 catgccggca atcgtggcct gcacgccggt gagcgtttcc gttacatagt ttatgaagaa
     1621 ggaattgacg ccggtctgcg cggccacata caggaactgc gcgaccacgg ccagaacaaa
     1681 tgccgggtgc cggaataact cacgggtagg tgcatccgct ttaatacctt cttcattttc
     1741 ctcctgtatg gccggcagcc gggtaaaggc aaaaatgata gccaccccca ccacaatggt
     1801 tccaacaagc atatagggtt tgagcaggga atcggacgat ccggtattgc cgggctgatc
     1861 ctgaccgaat atgaagaagc ctcccaaaag agggcccagt atccagccca gtccattgaa
     1921 ggattgagct atgttaatgc ggcgggccgc accgggcggg gggccaagta ccgtagtata
     1981 cggattggcg gctgtttcca ggcagcacaa accgcaggcg atgataaaga gcgcaatcag
     2041 gaaggaaata aataaagcgt ctttttcctg aatgtgcatt ttcactatgg ggaaaaataa
     2101 aaaagcgccc agggcaaata aggacaggcc cagtatgatc ccttttttat aaccataccg
     2161 tttcataaat aatccggccg gataagccat gacgagataa ccgatataga gggaaaactg
     2221 cacgaaaccc gattcagcct ttgatacatg cagggtatcc tgaaagtgct tgtcaagaac
     2281 gtcgagtaat ccgtgggcaa agccccagag taaaaacagg ctggtgatca aaataaaagg
     2341 tctgaggtaa ctggttccat cgatggcccg gagcaggccg ggaattttgt ttccttgttt
     2401 caggttcata tttgccatat tattttttca tacgctcatt caagcaattc ttaaatttaa
     2461 accattaaac tttcttccat catccgttcg tggatgatgg gggaaatatt tccggttttt
     2521 ccgccccgtc cctctatcgc gattcggatc atctcaggaa tacaatggtc cataagcggc
     2581 gcaggcccgg taatccgacc cttcagggtc tgaaccgaat gcggaccagg cggcgggacg
     2641 gaaaatttgt tcctccggca cattgtgcat gcataccgga atacgcagca tcgaagccat
     2701 tgagatcagg tcccggccaa tatgaccata gctgatggcg ccgtgatttg cgccccagtt
     2761 cgccatcact gaatatacat ctttgaaata ccctttgccg gtaaggcggg gaacgaacca
     2821 ggtggtcggc caggttctgt cagttctttc gtccagtgtc cggtgcactt cctcggggag
     2881 atcaatggtc agcccttccg caatctgcaa taccggtccg atgcctctca cgaggtttaa
     2941 gcggcacatg gtaacgggca tgcctccacg acttacgaat ttggaagaat atccgcctcc
     3001 ccggaaataa ccattattag cggggtacca ggtagtggcc tccagacagg cttccgcttc
     3061 gtccggcgtg atttcccaga atggcttcat ggcgggtttc ccgttccggc tttgcctgcc
     3121 ggttccgtcg agcgtgcagg cgccggaatt gatcaggtga ataaatccgt tcgctgcagc
     3181 cccggccggg ctccaaccgg aaactctttt caccgcttcc ggactccaat acgttcttac
     3241 atcggcaaag atctgtgccg tattggtgag cagatagttc aacagcatac ttatgccatt
     3301 gagggaatcg ttttcagtag ccacgataac cggtgaacgg atcccgttcc agtcgaacga
     3361 agaattcaga atggcttcag caaaatctcc gttcggtaaa aaatctgtcc attgccgctg
     3421 tccctgaaag cccgagacca atgcgttatg gccctgggac tcttctccaa atcctctttc
     3481 cttcaatagc ggattcccta tcatcaggtc ccggatgatc agggtcatct tcaccacgaa
     3541 agcccagtct ttgtcttttt gttcccggga aaactgtttt gaggccatat taaaatcttc
     3601 tccttctttg caatttgctt ttacccagga gatcgctttc tcaaattcag cctcactata
     3661 aattttcttt tctatccggc ggagcaattc agtagagtca acatattcat tgcgcatgcc
     3721 aaaatattcg cggaagagat ccggattaac catggatccg gcgatgccca tgcaaacgga
     3781 gccaacggcc agataagatt ttccccgcat aatggccacc gcgagcccgg cccgggcaaa
     3841 tccgagcaat cgctcctgca cgtcttccgg aatgtgttcg tcgccgaggt cctgcacgtc
     3901 ctttccatag ataccaaagg ccggaagccc catctggtta tgcgcggcca gggtggccgc
     3961 cagatagacg gctccgggac gttcggtacc gttgaatccc cagatcgctt ttggtatcca
     4021 tggattcagg tccatggttt cgctaccat
//
LOCUS       DASYCU010000554         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4324834, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000554 DASYCU010000000
VERSION     DASYCU010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4324834"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGM24_11190"
     CDS             <1..237
                     /locus_tag="VGM24_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155988.1"
                     /translation="SDMLPKKAVSSVIGIGSMAGAVGGVLFPMLIGFILDYYKGIHHI
                     TAGYNIIFVFCSLSFLLAWLLVHFITPKMEPVDF"
     gene            288..>2683
                     /locus_tag="VGM24_11195"
     CDS             288..>2683
                     /locus_tag="VGM24_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY4155989.1"
                     /translation="MKRMLTLACCLSSLFLFSQNSTIYHSGWIDFNKNGRMDIFEDTT
                     QPVEKRVADLLSQMTLEEKTCQMATLYGYGRVLKDEMPVAAWKQAVWKDGIANIDEEF
                     NGVPFHPEAATEYSYPFSKHARAINTIQKWFVEQTRLGIPVDFTNEGVHGLCSDRATP
                     LPAPISIGATWNRELVRHAGEIVGREAKALGYTNVYAPILDPARDQRWGRVVECYGED
                     PFLVAELGKQMTLGIQSQGVASTLKHFAVYSVPKGGRDGEVRTDPHVAPREMQQLFLY
                     PFRRVIEEAHPLGVMSSYNDWNGEPITGSHYFLTELLRQQFGFKGYVVSDSRAVEYLY
                     EKHHVAKDFKDATRQAVEAGLDVWTNFAMPDKYINNIRELVKEGLLSDKIVDQRAGEV
                     LRVKFLLGLFNQPYVSHPEEADQRVHTAEDEAFSLEINRQSLVLLKNKDNLLPLDRHS
                     AKRILVTGPLADATKYAVSRYGPGNNPVTSVYQGIRQILGERAQLKYEKGCEVVNAGW
                     PETEIINSPLSPEDKSSIQKAVEQARRSDIVIAVLGEDEKTVGEGLSRTSLELPGRQE
                     QLLEALYATGKPVVLVLINGQPLTINWANKYIPAILDAGFPGPSGGHAIASALFGDYD
                     PGGKLSMTWPKTVGQIEFNFPFKPGSQAAQAPKGRNGAGNTLVNGALYPFGYGLSYTR
                     FDYSNLQVDPATRHAQGKIQVAVDVSNSGSRAGDEVVQLYVKDKLSSVITYEMDLRGF
                     ERISLQPGEKKTVKFTLGPDDLALLDKNMHWTVEPGEFEVMIGSSSEDIRLRKSFLIE
                     "
BASE COUNT          677 a          657 c          757 g          592 t
ORIGIN      
        1 tccgatatgc tgccaaagaa agcggtgagc tccgtgatcg gtatcgggag catggccggc
       61 gccgtgggcg gtgtattgtt tcccatgctg attggcttca tacttgatta ttacaaaggg
      121 attcaccata tcacggcagg atataatatc atattcgttt tctgcagcct ttctttcctg
      181 ctggcctggc tgctggttca ttttattacg ccaaaaatgg aaccggtgga tttttaaccg
      241 gaagggttgg tgaatgggtt tgattttcta atgtgtaaat ggacaaaatg aaacgaatgc
      301 tgacgctggc ttgctgtttg agcagccttt tcctgttttc gcaaaattca acgatctatc
      361 attccggctg gatcgacttc aacaaaaacg gccggatgga tatttttgaa gataccacgc
      421 agccggtgga gaagcgggtc gccgatcttt tatcgcagat gaccctggaa gagaagacct
      481 gccagatggc caccctgtat ggatacgggc gggtgttgaa agatgaaatg ccggtcgcag
      541 cctggaagca ggccgtctgg aaggacggga tcgccaatat agacgaagaa ttcaatggcg
      601 ttccctttca tccggaagcc gcaacagaat attcctatcc cttcagcaag catgcccggg
      661 ccatcaatac catacaaaaa tggttcgttg aacaaacgcg gttgggcatc cccgttgatt
      721 ttaccaatga aggcgttcac ggcctctgca gtgaccgggc aacccccctg ccggccccca
      781 tttccattgg cgcgacctgg aacagggagc tggtccgcca tgccggagaa atagtggggc
      841 gtgaagcaaa ggcactgggc tataccaatg tgtatgcgcc tattctggat cctgcgcggg
      901 accagcgttg gggacgagtg gtggaatgtt acggggaaga tcctttcctg gtagccgaac
      961 tcggtaaaca gatgaccctc ggtattcaga gtcagggcgt ggcttccacg ctgaaacatt
     1021 ttgccgtata cagtgtgccg aaaggaggcc gcgatgggga agtaaggacc gatccccatg
     1081 ttgcgccccg ggaaatgcaa cagcttttcc tctatccgtt cagacgggtg attgaagaag
     1141 cccatccgct gggcgtgatg agcagctaca atgactggaa cggcgagccc ataaccggca
     1201 gccattattt tctcaccgaa ttgctccggc agcaattcgg ctttaaagga tatgtggttt
     1261 cagacagcag ggccgtggaa tatctttatg aaaagcatca tgtggccaaa gactttaaag
     1321 atgccacgcg ccaggcggtg gaagccggac tggatgtatg gacaaatttt gccatgccgg
     1381 ataaatacat taacaatatc cgcgagctgg tgaaggaggg tctgctctcc gataaaatag
     1441 ttgaccagcg cgccggagag gtgttgcggg taaaattttt gctgggatta ttcaatcagc
     1501 cctatgtttc ccatccggag gaggccgatc agcgggtgca tactgcggag gatgaagctt
     1561 tttccctgga aattaaccgg caatccctgg tgctgctcaa aaataaggat aacctgttgc
     1621 cgctggaccg ccattccgcg aaaagaatac tcgttaccgg accattggcc gatgctacta
     1681 aatatgccgt cagccgatat ggtcccggca acaatccggt cacctcggta taccagggca
     1741 tccggcagat actgggtgaa cgggcacagc tgaaatatga aaaaggctgc gaagtggtga
     1801 atgccggctg gccggaaacg gaaatcatca acagcccgct gagcccggag gataaaagca
     1861 gtatacaaaa ggcagtagag caggcccgga gatcggatat cgtcatcgct gtattgggcg
     1921 aagatgaaaa gaccgttggg gaaggactct cgcgcacttc gcttgaattg cccggacgcc
     1981 aggagcaatt attggaagcc ctgtacgcaa cgggcaagcc cgtcgtgctg gtgctcatca
     2041 acgggcagcc gctaacgatc aactgggcta ataaatatat accggccatc ctggatgccg
     2101 gtttccccgg accgtcggga ggccatgcca tcgcatcggc cctgtttggc gattatgatc
     2161 cgggaggcaa actgagcatg acctggccga aaaccgttgg acagatcgaa ttcaatttcc
     2221 ctttcaaacc cggttcccag gccgcgcagg ccccaaaagg acgcaatggc gcagggaata
     2281 cactggtgaa cggcgcgtta tatcctttcg gatacgggct aagctatacg cgtttcgact
     2341 atagcaatct gcaggtggat ccggcgaccc ggcatgcaca gggaaagatc caggtggcgg
     2401 tggatgtttc caacagcggc agccgggccg gcgatgaagt ggtccaatta tatgtcaaag
     2461 acaaacttag cagcgtgatc acctacgaaa tggatttaag gggatttgaa cggatcagcc
     2521 tgcaacccgg ggagaaaaaa accgtgaagt tcacccttgg acccgacgat ctggccctgc
     2581 tggataagaa tatgcactgg accgttgagc ccggcgaatt cgaagtgatg atcggaagct
     2641 cttccgaaga tatccgctta cggaaatcat ttctgattga ata
//
LOCUS       DASYCU010000555         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5045146, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000555 DASYCU010000000
VERSION     DASYCU010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5045146"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGM24_11200"
     CDS             <1..186
                     /locus_tag="VGM24_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolactonase"
                     /protein_id="HEY4155990.1"
                     /translation="QLGRVNAILPVPENKVYNLCFGGPDFDTLYIAAGDKVFRRKVKV
                     HGANGFQPAVKPPMPKL"
     gene            complement(224..1594)
                     /locus_tag="VGM24_11205"
     CDS             complement(224..1594)
                     /locus_tag="VGM24_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY4155991.1"
                     /translation="MKIINPATEELIEEIAEDNRYSLSNKFQSLKQSQNHWQQVRLAD
                     RIKPVQRFSELLEINRERLAEILTSEVGKPMQQSRNEINGAITRIQWLTQNAEKYLNE
                     EWMISGNNPEEKIVYEPLGVICNISAWNYPYLVGINVFVPALMAGNSVMYKPSEFATL
                     TGVEIDKLLKEAGIPDLVFQLAIGAGAVGEALLQLPFDGYFFTGSYKTGKSVYEKVSL
                     KMVPCQCELGGKDPLYVADDVEDIKAVAVSTADGAFYNCGQSCCSVERIYVHEKIYDA
                     YVDEFVREVRTWKMGLPTGEGVFIGPLSRKDQLAVLDQQVREAVRKGAVVLTGGKRPA
                     HKGYYFEPTVLVNVTNDMSLMQEESFGPMIGIMKVGNDEEAFKWMQDTVYGLTASVYS
                     ASRQRAEAILQKLNVGTGYWNCCDRVSAAVPWSGRQHSGIGATLSHIGLRAFTKTKAY
                     HLKRPG"
     gene            complement(1605..>1822)
                     /locus_tag="VGM24_11210"
     CDS             complement(1605..>1822)
                     /locus_tag="VGM24_11210"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY4155992.1"
                     /translation="LQLNEGGAETVINYLHELNARIGIPARLKDIGVKEEHIDSLADL
                     AFADFAHPNNPKPVSRGDFKKLYQRAL"
BASE COUNT          430 a          477 c          393 g          522 t
ORIGIN      
        1 cagctgggca gggtgaacgc tattttaccg gttccggaaa acaaagtata taatctttgc
       61 tttgggggtc ccgattttga taccctttat attgctgcgg gcgacaaagt attccggcgg
      121 aaagtgaaag tgcatggagc caacggtttt cagccggcgg taaaaccacc gatgccaaaa
      181 ctttaatcgt tccgtttcaa tccagtctcc agtgagattt tgtttaaccc ggccttttca
      241 aatgataggc cttggttttt gtgaaagcac gcaggccaat atgtgaaagc gttgctccta
      301 ttccggaatg ctgccttccg ctccagggaa cagcggcgct tacccggtcg caacagttcc
      361 aatagccggt tcccacattt aatttttgca gaattgcttc ggcacgctgc ctgctggcgc
      421 tgtaaacgga agcggtaagg ccgtataccg tatcctgcat ccatttaaaa gcctcttcat
      481 catttccgac cttcataatt ccgatcatgg gcccgaaaga ttcttcctgc ataaggctca
      541 tgtcgttggt cacattgacc aaaacggtag gttcaaaata atatcctttg tgtgctggtc
      601 ttttgcctcc ggtgagcaca acagctcctt tccggacggc ctctctgacc tgctgatcca
      661 aaacggccag ttggtcttta cggctcaggg ggccaatgaa tacgccttcc ccggtgggca
      721 aacccatctt ccaggttcta acctcccgca cgaactcgtc cacataagca tcatagatct
      781 tttcgtgtac ataaatccgt tccaccgaac agcaactctg gccacaattg taaaatgcac
      841 catctgctgt actgaccgcc acggctttaa tgtcttcaac atcatctgca acatacaagg
      901 ggtctttacc tcctaactca cattgacagg gaaccatttt taaagacact ttttcataaa
      961 cagacttccc cgttttataa ctgcccgtaa agaaataccc gtcaaacggg agttgcagaa
     1021 gggcttcgcc aacggctccg gcaccgatgg ccagctggaa aaccaggtcg gggatgccgg
     1081 cttcctttaa taatttatcg atctccaccc cggtgagcgt ggcgaattca gagggcttgt
     1141 acatcaccga attcccggcc atcaatgccg gcacaaatac atttattccc accaggtaag
     1201 gataattcca ggcagaaata ttgcaaatca ctccgagcgg ctcgtataca attttttcct
     1261 caggattatt cccggatatc atccactcct cgtttaaata tttttctgcg ttttgggtga
     1321 gccattgaat tcttgttatg gctccgttga tttcatttct ggattgctgc atgggtttac
     1381 cgacttcact ggtaagtatt tcggcaagac gctcccggtt gatctctaaa agctccgaga
     1441 aacgctgaac cggcttgatt ctgtctgcca gtcggacctg ttgccaatgg ttctgcgact
     1501 gcttcagcga ttggaattta ttgcttaacg aataccggtt gtcctccgct atttcctcaa
     1561 tcaattcttc tgtagccgga ttgatgattt tcatggttaa ttcattatag cgcccgttgg
     1621 tataattttt taaaatcccc cctgcttacc ggtttgggat tattcggatg cgcaaaatcg
     1681 gcaaacgcaa gatcggccag gctgtcgata tgctcttctt tcacgccgat gtctttcagg
     1741 cgtgccggaa taccgatcct ggcatttagt tcgtgcaaat aatttattac tgtttccgca
     1801 cccccttcat ttaattgcag tg
//
LOCUS       DASYCU010000556         3574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_554554, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000556 DASYCU010000000
VERSION     DASYCU010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554554"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VGM24_11215"
     CDS             complement(<1..823)
                     /locus_tag="VGM24_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY4155993.1"
                     /translation="MKKIAVIGAGTMGNGIAQVFSQYGFQVNLVDLHTGQLEKAISRI
                     DKNLNRQLEKGTLTAEAKRETLDRINTFSDLKSGVSAAELIIEAVSENAGIKLNIFRE
                     LDKEALPGAILASNTSSLSITRIAAATANPSRVIGMHFMNPAPVMKLVEVINGYSTSS
                     SVTRTVTEIARQLEKVPCVVNDYPGFIANRILMPMINEAIISLQEGVAGVEAIDTVMK
                     LGMAHPMGPLQLADFIGLDICLSILTVLHQGFGNPKYAPSSLLINMVNAGVLGVKS"
     gene            892..2187
                     /locus_tag="VGM24_11220"
     CDS             892..2187
                     /locus_tag="VGM24_11220"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY4155994.1"
                     /translation="MYPNLYYAFKDLFGVDWKFLHFINSFGFFVAISFILAALVLTRE
                     LKRKEGQGWLQAEEEIRVVGRPASPGEIFTNFILGFIFGYKFIGLFTAGGPVSQNPAD
                     FIFSSRGNWLAGIALGLLFAGMKFWEKNKQKLPEPEERRIRIWPHDRVGDIIILGAIF
                     GFVGAKIFNSLETWDEFVKNPIESLISFSGLTYYGGLICAALAIWYFARKHKIGIWHL
                     NDAAAPALMLAYGTGRIGCQTAGDGDWGITNSAYAVDANLKTVAAGADNFHQVVSRNA
                     DYYMSQFQTLNVPHLSVKGPSFLPDWFFAYAYPHNVINSGIKIPGCTGDYCSYLPLPV
                     FPAPFYETIACIGLFFLLWSLRKKFKAPGTLFAVYLILNGIERFLIEKIRVNTRYSIL
                     GFHPTQAEIISTLLVITGIILYFIMKKRYKAKLEQNYPV"
     gene            2395..>3574
                     /locus_tag="VGM24_11225"
     CDS             2395..>3574
                     /locus_tag="VGM24_11225"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4155995.1"
                     /translation="MKLLFLGVFTALSCLVNAQTPTASNIRGTVNEENQSPVEAASIA
                     LLKEADTARYKMSATDKSGRFIFESVPAGNYHLLVTAVGHEPFSGSSFTVKPGKKNYD
                     LPAVVLKKESKELQSVAVVGKKSFIEQKADRMVVNVDASPSNAGSSAMDVLEKSPGVS
                     VDKDGNISLKGKQGVTVMVDDKPTYMTAAQLASYLKGLPASALDQIEIMTNPSAKYDA
                     AGNSGIINIKTKKNKAKGFNGSLTLTQTQSKYGKPGGSLNLNYRTGKVNFFFNGGFNR
                     WKGWHDLDINRNYLNAGDKQINSIFTQHTHMEYSSPQFNGKAGMDYYINDKTTVGFVV
                     SGYQSNEKNESQSYITLKNANNVPDSLVNSFGNINGKWQSASVNLNFMRKFDSLGTEL
                     T"
BASE COUNT          957 a          851 c          847 g          919 t
ORIGIN      
        1 cggatttcac gcccaggaca ccggcattca ccatgttaat cagcagggaa gaaggcgcat
       61 atttcggatt accgaagccc tggtgtaaga ccgttaaaat agaaaggcaa atatccaacc
      121 cgataaaatc cgccagttgc aggggtccca tcggatgagc catgccgagt ttcattacgg
      181 tatctatggc ttccaccccg gcaacgcctt cctgaagact gatgatggcc tcgttgatca
      241 tcggcatcag aatgcggttc gcaatgaatc caggataatc attcaccaca cagggaacct
      301 tctccagctg gcgggcgatc tcggtaacgg tacgggtaac cgaagaggag gttgaatatc
      361 cgttgatcac ttcaaccagt ttcattaccg gagcgggatt catgaaatgc ataccgatca
      421 cccgggaagg atttgccgtt gccgcagcaa tacgggttat ggagagggaa gacgtgttgc
      481 tggccagaat ggctccgggc aacgcctctt tatccagctc ccggaaaata ttcaatttga
      541 tcccggcatt ttcggaaaca gcttctatga tgagctcagc agccgacacc cctgatttca
      601 gatcggaaaa agtatttatg cgatccaggg tttcccgttt tgcttctgca gtcagcgttc
      661 ctttttccag ttgacggttc aggtttttgt caatgcggct gatggccttc tccaactggc
      721 cggtgtgcag gtcaacgagg ttcacctgga aaccatactg actaaacacc tgagcaatac
      781 cgttacccat ggtgccggcc ccgatgacag ctattttttt cataaccggt attattttgg
      841 aacaagttta cgaagcttcc gtcaactttt tagccttatc ttgcaactcc tatgtatccc
      901 aatctgtatt acgcttttaa ggatcttttc ggtgttgact ggaagttcct gcattttata
      961 aacagcttcg gtttttttgt cgcgatctct tttatcctgg ccgccctggt gttaacgcgg
     1021 gagctgaaac gcaaggaagg acaggggtgg ctccaggccg aagaagagat ccgggtggtc
     1081 ggaagaccgg ccagccccgg tgagattttc accaatttca tactcggatt tatattcgga
     1141 tataagttca tcggattatt tacggccggc ggaccggtat cacaaaatcc cgcggatttt
     1201 attttctcct cccggggaaa ctggctggct ggtattgccc tgggattgct gttcgccgga
     1261 atgaaattct gggaaaaaaa taaacagaaa cttcctgaac cggaggaaag aaggatccgg
     1321 atctggccgc acgaccgggt aggggatatc atcattttag gagccatctt cggttttgtc
     1381 ggcgcaaaaa ttttcaacag cctcgaaacc tgggatgaat ttgtaaaaaa tccgatcgaa
     1441 tcactcatct ctttcagcgg cctcacgtat tacggcggac tgatctgtgc cgccctggcg
     1501 atctggtatt ttgcccgcaa acataagatc ggcatatggc atctgaacga tgccgccgcc
     1561 cctgcgctga tgctcgctta tggaacgggt agaattggct gccagactgc cggcgacggg
     1621 gactggggaa ttaccaacag cgcttatgcc gtcgatgcca atttgaagac cgttgccgcc
     1681 ggtgcagata attttcatca ggtcgtttcc aggaatgcgg attattatat gagccagttt
     1741 caaaccctga atgttccgca cctctcggta aaagggccct cgttccttcc cgattggttt
     1801 ttcgcctatg cctacccgca taatgtgatt aattcaggaa ttaaaattcc gggttgcacg
     1861 ggtgattatt gcagttatct gcccctgccc gtattccccg caccatttta tgagacgatt
     1921 gcgtgtatcg gcttattctt cctgctctgg tctctccgca aaaaattcaa agccccgggt
     1981 actctattcg ctgtatacct gatattaaac gggattgagc gattcctgat agaaaaaatc
     2041 agggtgaata cccggtattc tatcctggga ttccacccga cgcaggctga aattatttcc
     2101 accctgctgg ttatcacagg aattatcctg tatttcatta tgaaaaaaag atataaggcc
     2161 aaattggagc aaaactaccc agtttaataa ccgttgctcc aggaatccca tccattcatc
     2221 agattttttc gttttgctgc aacttcttta gaatcattgc gtcctatccc caacaactat
     2281 tttccggtta tcagtaaaat cagccttcta acataaaaca atgatctatg ttatacttag
     2341 tactgatctt tgacctgtaa ctaccaaaaa atagtaccaa gtaaaccaaa aaacatgaaa
     2401 ttattatttt taggcgtatt tacagcgctt tcctgcttag tgaacgccca aacgccaact
     2461 gcttccaata ttcgcggaac ggtaaatgaa gagaaccagt cgccggtgga ggcagccagc
     2521 attgccctgc tgaaagaggc cgatacggca aggtataaaa tgagtgcgac ggacaaatcc
     2581 gggcggttta ttttcgaatc cgttccggcc gggaattatc atttgctggt tactgctgtg
     2641 ggacatgaac ctttttccgg ttcctccttt accgttaagc ccggcaaaaa gaactacgat
     2701 ttaccggcgg tggttcttaa gaaagaaagt aaggagctgc aatcggtggc ggtcgttggt
     2761 aaaaaatctt ttattgaaca aaaggcagac cggatggtgg tgaatgtgga tgcttcacct
     2821 tccaatgccg gatcatccgc catggacgtt ctggaaaaat cgccgggagt ttcggttgac
     2881 aaggatggaa atatcagcct gaaagggaaa cagggcgtaa cggtgatggt ggatgacaag
     2941 ccgacttata tgaccgctgc ccagctggcc agttacctga agggtctgcc cgcttcagcc
     3001 ctcgaccaga tcgaaatcat gaccaacccg tcggccaaat acgatgccgc cggaaactcg
     3061 gggatcatca atattaaaac caagaaaaat aaggccaaag ggttcaacgg ttcgttgacg
     3121 cttacccaaa cgcaaagcaa atacggtaag cccgggggca gcctgaacct gaattaccgg
     3181 accggaaaag tgaacttctt ttttaacggc ggattcaatc gctggaaagg atggcacgat
     3241 ctggatatta acagaaacta cctgaatgcg ggggacaaac agatcaattc catttttacc
     3301 cagcataccc atatggaata ttcaagtccc cagttcaacg gcaaagcagg aatggattat
     3361 tacataaatg ataaaacgac ggttggattt gttgtcagcg gatatcaaag caatgaaaaa
     3421 aatgaatccc agagttatat tacgctgaag aatgcgaata atgttcccga ttcgctggtg
     3481 aattcctttg gcaacataaa tggaaaatgg cagagtgctt ccgtaaacct caacttcatg
     3541 agaaaatttg attcactggg aacggaactc acgg
//
LOCUS       DASYCU010000557         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4759540, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000557 DASYCU010000000
VERSION     DASYCU010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4759540"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VGM24_11230"
     CDS             complement(<1..650)
                     /locus_tag="VGM24_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY4155996.1"
                     /translation="MADKKITSYPKEKINILFLENISDAAVKHFSNAGYTSVKKLTRA
                     LSEEELMKEIRDVHLLGIRSKTRITAKVLDAAQKLQAIGCFCIGVNQVDLKNAVRHGI
                     AVFNAPYSNTRSVAELVIGASVLLIRRIPDKSKAAHSGVWLKESKGSYELRGKTMGII
                     GYGNIGTQVSVLAEAMGMKVIFYDIVTKLPLGNAEARKTLKELVSESDIITLHVPDT"
     gene            801..2009
                     /locus_tag="VGM24_11235"
     CDS             801..2009
                     /locus_tag="VGM24_11235"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY4155997.1"
                     /translation="MKTFCCTLMAASFLTCCAQSPSSKGLVKNQDTQLLAARNPVPKE
                     LPAAEYHHFSNTSRHFFDSLFQHGFNGGLLVARDGEVVYERYSGFKNVRKKKDSVNAH
                     TAFHLASVSKTFTAMGVLKLWEEGKLDIHDDISRYLTGFPPGTTIKDLLSQRSGLANY
                     VHFMDNSGWDKRKFLTNADLLRYIIQHAATVQHLPPGRHFEYSNTNYAFLALIIEKVS
                     GQTYAEFITKTFFEPLQMHDSYVFSLQDTARATHSYKISGRPYPLEYLDVVYGDKNIY
                     STPEDLLKWDAALRGGILFKQSTLDSAYTPYSLEKPGKRNYGLGWRMIVYPNGKKLIY
                     HNGWWHGNRTVFIRMMEENATIIALCNNDNKRVYSSKKLCDAFGNYQQEHEAPDEAEN
                     DPEGRGGEKR"
     gene            2056..2952
                     /locus_tag="VGM24_11240"
     CDS             2056..2952
                     /locus_tag="VGM24_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY4155998.1"
                     /translation="MKDVASFLKDFHGKYALLDLEAARSIYRARDPLSNKGDFGHALL
                     LAGSYGKIGAALMAARACLASGAGLLTTHLPVSGYPIMQTALPEAMVSVDSDPHQITD
                     LPGDLKPYRAVGAGPGIGSSEPTSAMIKKLLKSFRHPLVLDADALNIISRDKKLLSKI
                     PPNSILTPHQKEFDRLFGSSKGDIQRFELAEKNAAKYQVILLLKGHYTGIFCPDGSIF
                     FNTSGNAGMAKGGSGDVLTGMVTAMLAQSYSPSASARLAVFLHGLAGDFAAGLYSEEA
                     MLPTDLIACIGKGFLQLKKTPG"
BASE COUNT          791 a          802 c          722 g          779 t
ORIGIN      
        1 gtgtccggca cgtgcagggt gatgatgtcc gactcagata ccagctcctt cagggttttc
       61 ctggcctcgg cattgccaag cggtagtttg gttacaatat catagaagat cactttcatg
      121 cccatggcct cagccagcac gctcacctgc gtgccgatat ttccataacc gataatgccc
      181 atggtctttc ccctgagctc gtaacttccc ttggattcct tgagccaaac cccggaatgc
      241 gccgctttgc ttttatccgg tatcctgcgg ataagcagga cggatgcgcc aatcaccagc
      301 tcggctaccg aacgggtatt ggaataaggc gcgttgaata cggctattcc gtgcctgact
      361 gcgtttttca gatccacctg gttaaccccg atgcagaaac agccgatggc ctgcaatttc
      421 tgggcagcat ccagaacttt cgccgtgatc cgggtcttgg accggatgcc cagtaaatga
      481 acgtcccgga tctccttcat caattcctct tccgataagg cgcgggtcag ttttttaaca
      541 gatgtgtaac cagcgttcga aaagtgcttt accgctgcat cactgatgtt ttccagaaat
      601 aaaatattga tcttctcttt gggatagctg gtaatttttt tgtcggccat aatctgaata
      661 gctgtttttg cgttatttcc ctgaaaggag ttgcaaaatt cccattaaaa tcggaaacgg
      721 aaaaacaaaa ctggtttcct taggtcaaaa tggcataata ttttattttt gagcttacaa
      781 aacgtttaaa ttaatacctg ttgaaaacct tctgttgcac cctgatggcc gcctcttttc
      841 tgacctgttg cgctcagtcg ccctcttcca aaggactggt gaaaaatcag gatacccagc
      901 tgcttgcagc caggaatccg gtccccaaag aacttccggc tgccgaatac catcatttca
      961 gcaatacctc ccgtcatttt ttcgactcct tatttcaaca tggttttaat ggaggcctgc
     1021 tcgtagcccg ggacggtgaa gtggtttacg aacgatacag cgggtttaaa aatgtccgga
     1081 aaaagaaaga ttccgtaaat gcccatacgg cctttcacct cgcctccgtt tcaaaaacct
     1141 ttaccgccat gggggtattg aagctttggg aagaaggaaa actggacatt cacgatgata
     1201 tctcccgtta cctgaccggc ttccctcccg gcactaccat aaaagacctg ctcagccagc
     1261 gcagcgggct ggccaactac gttcatttta tggacaattc gggatgggac aaaagaaaat
     1321 ttctgaccaa tgcagacctg ctgcgctata taattcagca tgcggcaacg gtgcagcacc
     1381 ttccccccgg ccggcatttt gaatattcga atacgaatta tgcctttctg gccctgatca
     1441 ttgaaaaggt ctccggtcag acctatgccg aattcatcac aaaaacattc tttgaacccc
     1501 tgcaaatgca cgatagttac gtattctcgc tgcaggatac agcccgtgcc acgcattcct
     1561 ataagataag cggccggcct tatccgctcg aatacctgga tgtggtctac ggcgataaaa
     1621 atatttacag cacaccggaa gacctgttga aatgggatgc ggccctgaga ggcgggatct
     1681 tatttaaaca gtccaccctg gacagtgcct atacgcccta tagtctggaa aaacccggaa
     1741 aacgcaatta tggattggga tggcggatga tcgtttatcc caacggtaaa aagctgattt
     1801 atcacaatgg ctggtggcac ggaaaccgga cggtctttat ccggatgatg gaagaaaatg
     1861 caaccatcat cgccctctgc aataacgaca acaaaagagt ttattcctcc aaaaaactct
     1921 gcgatgcctt cggaaattat cagcaggaac acgaggcgcc ggacgaggct gaaaatgatc
     1981 cggaaggccg tggcggcgaa aaacgctaag gccggccggg tgcggaatga ctgacttatt
     2041 cctaccttca tctgcatgaa agatgttgct tcttttctaa aagattttca tggaaagtat
     2101 gccctcctgg atctggaggc ggcccgctcg atctacagag cccgggaccc cctctccaat
     2161 aaaggcgact ttggacatgc ccttctgctg gccggcagtt atggaaaaat cggagccgcg
     2221 ctgatggctg ccagggcctg cttggccagc ggcgccggat tgctgaccac ccatctcccc
     2281 gtaagcggtt atccgattat gcagaccgct ctgccggaag ccatggttag cgtagattcg
     2341 gatcctcatc agatcacgga tttacccgga gatttgaagc cttaccgggc cgtgggtgca
     2401 ggtcctggaa tcggcagcag cgaaccaacc agcgcgatga ttaaaaaatt gcttaaatca
     2461 ttccgccatc ccctggtact cgatgcagat gcgttaaata ttatcagccg ggataaaaaa
     2521 ctcctttcaa aaattccccc gaattccatt ttaaccccgc atcaaaaaga gttcgaccgt
     2581 ttattcggtt cctccaaagg agatatccaa cgatttgaac tagctgaaaa aaacgccgca
     2641 aaataccagg tgatcctgct tttgaaaggc cattataccg ggattttttg tccggacggc
     2701 agcatctttt ttaataccag cggaaatgcc ggtatggcta aaggcggaag cggggatgtg
     2761 ctcaccggca tggtcaccgc catgcttgcc cagtcttatt ctccttcagc atcagcccgg
     2821 ctcgctgttt ttcttcacgg tctggccggg gattttgcgg ctggacttta ctcggaagaa
     2881 gccatgctgc caacggatct catcgcctgc atcggcaaag gttttctgca gttgaaaaaa
     2941 actccgggat aaccggccac cctgaattta tttaataata ttttgaataa tggaggacac
     3001 caattcaaaa tcagttgact tatccaggaa atgatcggca cccaggacct ggcataactt
     3061 tctgtagtaa gaaccggact ggttggtgag catg
//
LOCUS       DASYCU010000558         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2438008, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000558 DASYCU010000000
VERSION     DASYCU010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2438008"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..1763
                     /locus_tag="VGM24_11245"
     CDS             369..1763
                     /locus_tag="VGM24_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4155999.1"
                     /translation="MAERLKLREKIGYGFGDMASSMFWKLFGTYLLFFYTDVFGIAAT
                     AVGTMFLITRVWDTFFDPVVGVIADRTKSKWGKFRPYLLYVSVPFGIIGVLTFTVPSF
                     SGTWTLVYAYITYSVMMMIYSLINVPYASLLGVISSDPKQRNILASFRMAFAFAGSLV
                     ALALIEPLVNIFSKRAGGAPDLRAGWQLAVVVIAAICVLLFIGCFSWVKERVQPLKQE
                     KSSLKNDVRDLIQNRPWWILLGAGVAALIFNSIRDGATIYYFKYVIQNTEAFKIGTTG
                     ISWSTLYLIVGQAANIIGVILASPVANRAGKKNTFLAAMGTATVLSIIFYWLNAHQIL
                     LIFIFQFFISVCAGIIFPLLWSMYADIVDYSEWRTGRRATGLIFSSSSMSQKLGWTVG
                     GALTGWLLGAYGFKANVVQGADVQTGIRMMLSFLPAAGALLSVFFIALYPLSEKRVIG
                     IAGELEEKRKNSIL"
     gene            complement(1752..>2476)
                     /locus_tag="VGM24_11250"
     CDS             complement(1752..>2476)
                     /locus_tag="VGM24_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY4156000.1"
                     /translation="YLFCDEFTNYNDTETGIKAVLLLEKLGYEVIIPEHDESGRAWMS
                     KGLLKAAKKIANRNIASLAKIVTAENPLIGIEPSAILTFRDEYIDLADDENWEAAKAL
                     SKNVLLIDEFISREIEKGNIDHTLFTKEKRQVKLHGHCQQKALSSLSASVKMLSLPEN
                     YTVETIPSGCCGMAGSFGFEKEHYDLSMKIAELVLLPAVRSQGEDVIIAAPGTSCRHQ
                     IKDGAQRKALHPVEVLFEALKD"
BASE COUNT          559 a          585 c          635 g          697 t
ORIGIN      
        1 atggagtttc tatcgcttct ttcaccagct gataccggtc aacgacctcc cggagtaagg
       61 ggtccggtct gaaagattgg gtatcaagca taaattaatt tatcccatcg ggataccgga
      121 tatatgggga ttcagcaggg gccggattca gccggagtag ccccggaaga taagaaattc
      181 ccgggatgcg ttttcaaatc ctgcccggta tttaaccgtt tcccacgccg ggccggcgaa
      241 acgattctca ggcccgtccc gcgggctttg ttaaaaaagt atcacagtat ggtgattaag
      301 gtcatccggc tcccggttaa tggaattatc tttattttca tcagggatcg gaacaatcaa
      361 aattaactat ggcggaacgg ttaaaactca gggagaaaat tggatatggt tttggcgaca
      421 tggcttcctc catgttctgg aaattattcg ggacctacct gctgtttttt tataccgatg
      481 tttttggaat tgctgccaca gccgtaggga ccatgttcct gatcacccgg gtctgggaca
      541 ctttttttga cccggtggtc ggggtgatcg cagaccggac aaaatccaaa tggggtaaat
      601 tccgtcctta cctgctttac gtatccgttc cgtttggaat aatcggcgtg ctgacgttta
      661 ccgtcccttc tttttccgga acctggaccc tggtctatgc gtatatcacc tattcggtaa
      721 tgatgatgat ctattcgctg atcaatgtgc catatgcgtc tttactgggt gtaatatcgt
      781 ctgatccgaa gcagcgcaat atactcgcct cattccggat ggcctttgcc ttcgccggca
      841 gcctggtcgc gctggcgctg atcgaacccc tggtgaatat attcagcaaa agggcgggcg
      901 gggcgccgga tctgcgggca ggctggcaac tggcggtcgt cgtaatagcg gccatttgcg
      961 tactgctgtt catcggctgt ttttcctggg taaaagaaag ggttcagcct ttaaagcagg
     1021 aaaaatcatc tcttaaaaat gatgtccggg accttattca gaaccggccc tggtggatcc
     1081 tgctgggggc gggcgtggcg gccctgatat tcaactccat ccgggacggg gccacgatct
     1141 attattttaa atacgtcatt cagaatacgg aagcatttaa gattggcacc accgggataa
     1201 gctggtcaac gttatatctg atcgtcggac aggcagccaa tatcattggc gtgatcctgg
     1261 cctcaccggt ggctaaccgg gcaggcaaaa aaaatacgtt tctcgcggcc atgggaacgg
     1321 ctaccgtcct gagcattatt ttttactggc tgaatgccca tcagatcctg ctgattttca
     1381 tttttcagtt ctttatcagc gtttgtgcgg gcatcatttt tcctttatta tggtccatgt
     1441 acgctgatat tgtggactat tcagaatgga ggaccggaag acgggcaacc ggtctgattt
     1501 tttcttcttc ttccatgtcg cagaagctgg gctggacagt cgggggagca ttaaccggct
     1561 ggctcctggg tgcatatggt ttcaaggcca atgtggttca gggagctgac gtgcagaccg
     1621 gcatccggat gatgctgagc tttctgccgg cggcaggcgc tttattatcg gtatttttta
     1681 ttgccttata cccgctctct gaaaagcggg ttatcgggat agccggtgaa ctggaggaga
     1741 agagaaagaa ctcaatcctt tagcgcctca aacaacacct caacaggatg taaggctttc
     1801 cgctgtgccc cgtctttgat ctggtgcctg caactggtgc ccggcgcggc aatgatcaca
     1861 tcttcgccct ggcttcgtac ggctggcaat agcacgagtt cggcaatttt catggagaga
     1921 tcataatgct ctttttcaaa accaaaagaa ccggccatgc cgcagcatcc ggaagggatg
     1981 gtttccaccg tataattttc ggggagagaa agcattttga cggaagcaga aagggaggac
     2041 aatgcttttt gctgacaatg tccgtgcaat ttaacctggc gtttttcctt tgtaaaaaga
     2101 gtatgatcaa tatttccctt ttctatttcc cgggaaatga attcatcgat cagcaaaaca
     2161 tttttagaca gggcttttgc cgcttcccaa ttttcatcat cagccagatc gatgtattcg
     2221 tcccggaaag ttaagatggc actgggttct ataccgatca gcgggttttc ggccgttacg
     2281 attttggcca gggacgctat attccggttg gcgatttttt ttgctgcttt caataatcct
     2341 ttggacatcc acgctctgcc gctttcatcg tgttccggga taatgacttc atatcccagt
     2401 ttttccagca gcaggacggc tttgataccg gtctccgtat cattgtagtt ggtaaattca
     2461 tcgcagaaca ggtata
//
LOCUS       DASYCU010000559         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3310177, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000559 DASYCU010000000
VERSION     DASYCU010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3310177"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2917)
                     /locus_tag="VGM24_11255"
     CDS             complement(<1..2917)
                     /locus_tag="VGM24_11255"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEY4156001.1"
                     /translation="MNFDLMYPSVIKLIMKSVGHGRFLFMLCLLAGSGELSGQDLKAE
                     TMRPAADQRTNSFYVSDRRPLQKERFIKLPLTAFSPGGWLKKQLDLQRDGLTGHLGEI
                     SIWLSRKDNAWLDKNGKGKWGWEELPYWLKGYGDIAYMLKDQRMIRETKFWINAVLNN
                     QRENGDFGPMVVHKGTRDLWTNMPMLWCLQSYYEFSNDKRVLHLMSRYFKWELSLPDE
                     HFLEDYWENSRGGDNLYSVYWLYNRTGEPWLLDLASKIDRNTADWRQQNNLPNWHNVN
                     IAQCFREPATYYLQSHNSKDLEATYNDFKLVRNIYGQVPGGMFGADENARKGYDDPRQ
                     AVETCGMVEQMTSDQMLLRMTGDDSWAENCEDVAFNTFPAAFMPDFRSLRYLTAPNMV
                     VSDSKNHSPGIDNNGPFLMMNPFSSRCCQHNHSAGWVYYAENTWMATPDDGLVAQLYS
                     DGSVTASVGDGTNVVFTEQTHYPFGETVNFSVKASKPVRFPLYLRIPAWCGAAQVKVN
                     GKEVNISPEAGTYLRLTKKWKSGDRIILQLPMKIKLRTWEKNKNSVSIYYGPLSFSLK
                     IKERYVRKDSKETATGDSKWQANADAGKWPSFEIYPASAWNYGLLLEPGEPEKSFTVK
                     RNPWPADDNPFTNESAPIELEAKGKEIPSWTLDQYGLCAPLPQSPVVSRSATVPLELV
                     PMGGARLRISAFPVLVPDQPAAGRSLINPLLPSGPDPYCFYKDGYYYYTHTLGNRIDL
                     WKTKTMADLKTAERKTIFVPAAGTFYSKELWAPEVLFLQGKWYAYFAADNGSNKNHRL
                     YVLENGSADPMQGEWALKGKLADPADKWAIDADVFVFRGQMYMVWSGWEGDTNGEQDI
                     YIAKMKNPWTVEGNRVQISKPVYEWEKFGDLKNSSGPQHVNVNEGPQALIHGNDLFVL
                     YSASACWTDTYSLGLLRFGGDSDLLNGKNWIKSPHPVFRQSKENGVFATGHNSFFKSP
                     "
     gene            3065..>3623
                     /locus_tag="VGM24_11260"
     CDS             3065..>3623
                     /locus_tag="VGM24_11260"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY4156002.1"
                     /translation="MKPTFEPLVSSANTSFLVRHFEEKCFSAPYHFHPEYELTWIVEG
                     SGKRYVGTHMHDYFPGDLVLLGSNLPHCWKTENSKAGENSSSVVVQFHPDFMGKDFFS
                     RPEMKYIHRLLTNSQSGIQFTGNTENLKEKMNRMLDENHSFRKWALLMDILNELASTK
                     EYSLLSQEAPAPELSVLEKARMNTCI"
BASE COUNT          877 a          941 c          844 g          961 t
ORIGIN      
        1 cgggcgattt aaagaatgaa ttgtgaccgg tggcaaaaac gccattctct ttggattgcc
       61 tgaatacggg atgtgggctt tttatccagt tctttccgtt cagcagatca ctatcgccgc
      121 caaaccggag caaccccagg gaataagtgt ccgtccagca cgcactcgcg gaatacagca
      181 cgaacaaatc attcccgtga atcaatgcct gaggtccttc attcacgttg acatgctgcg
      241 ggccggacga atttttgagg tctccaaact tttcccattc gtaaacgggt ttactgatct
      301 gcacgcgatt tccctccacg gtccagggat ttttcatttt tgcgatatag atatcctgtt
      361 ccccattggt atcgccctcc caaccggacc agaccatata catctggccc cggaacacaa
      421 atacatccgc atcaatggcc catttatcgg cgggatccgc cagtttccct ttaagcgccc
      481 attctccctg catgggatcg gccgaaccgt tttccagtac atacaggcgg tgatttttgt
      541 tactaccgtt atccgcggcg aaataagcat accatttccc ctgaaggaat aacacttcgg
      601 gcgcccataa ttcttttgaa tagaatgttc ctgctgctgg cacaaatatg gtttttcttt
      661 cagccgtttt cagatctgcc atcgtttttg ttttccacag atcgatccgg tttcccaggg
      721 tgtgggtgta atagtagtat ccgtccttat aaaaacaata gggatccgga ccggaaggaa
      781 gaagcggatt tatcaaactg cgcccggcag ccggctgatc cgggaccagc accggaaaag
      841 ccgatatccg gagtcttgcg cctcccatcg gaaccaattc caggggtacg gttgccgacc
      901 gggaaactac cgggctttgc ggaagcggtg cacataaacc atattgatca agcgtccagg
      961 agggaatttc ctttcctttc gcttccagtt caatgggcgc cgattcattc gtgaatggat
     1021 tgtcgtctgc gggccaggga tttctcttta ccgtaaagga tttttccggt tctccgggtt
     1081 ccagtaacaa accataattc caggcgcttg ccgggtaaat ttcaaaggag ggccacttcc
     1141 cggcatctgc atttgcctgc catttcgaat cgccggtggc tgtttcctta ctgtccttcc
     1201 ggacatatcg ttctttaatt ttcagagaaa aactcagggg gccgtaataa atactgacgc
     1261 tgtttttatt tttctcccac gtgcgcaatt ttattttcat gggtaactgc agtataatgc
     1321 ggtcgccgct tttccatttc tttgtcaggc gcaggtaagt tccggcttcg ggagaaatat
     1381 ttacctcttt tccgttcact ttcacctgtg cggcaccgca ccaggccgga atgcgcaaat
     1441 acaaaggaaa ccggaccggc ttcgacgctt tcacagaaaa atttaccgtt tccccaaaag
     1501 gataatgggt ttgttccgta aatacaacgt ttgttccgtc tcccacgctg gccgtcacgg
     1561 aaccatcgga atacaactgc gcaacgaggc cgtcatcggg agtggccatc caggtgtttt
     1621 cggcataata aacccagccc gcagaatgat tgtgctgaca gcatcggctg ctgaaggggt
     1681 tcatcatcag gaaggggcca ttattatcga tgccgggaga atgattttta ctgtcgctga
     1741 ccaccatatt gggcgcggtt aaataacgaa gcgatctgaa atccggcatg aaggccgccg
     1801 gaaatgtatt gaaggcaacg tcttcacaat tttcagccca ggagtcgtct cccgtcatcc
     1861 ggagcagcat ctggtctgac gtcatttgct ccaccatgcc gcaggtttcc acggcctggc
     1921 gcggatcgtc atatcctttt cgcgcgttct catccgcgcc gaacatgccg ccgggaacct
     1981 gtccgtaaat atttctcacc agcttaaaat cattgtatgt cgcctccagg tctttgctgt
     2041 tgtgactctg caaatagtag gtcgccggtt cccggaaaca ctgcgcgata ttcacattgt
     2101 gccagttggg caggttattc tgctgccgcc agtcggccgt gttacgatct atttttgagg
     2161 ccagatccag cagccagggc tccccggtac ggttgtataa ccagtatacg ctgtagaggt
     2221 tatccccgcc acggctgttc tcccaataat cttccaggaa atgttcatcg ggcagagaga
     2281 gttcccattt aaaatatctg ctcatcaaat ggagtacgcg tttatcatta gaaaattcgt
     2341 aataggattg caggcaccag agcatgggca tattggtcca tagatccctt gtccccttat
     2401 gcaccaccat cggcccgaaa tccccgttct cccgttggtt gttaaggaca gcatttatcc
     2461 agaattttgt ttccctgatc atccgctggt ccttcagcat ataggcgata tcgccatatc
     2521 cctttaacca gtagggcaat tcttcccatc cccatttgcc ctttccgttt ttatccagcc
     2581 aggcattatc tttcctggaa agccagatgc tgatttcgcc cagatggccc gtaagaccgt
     2641 cgcgttgtaa atccagctgt tttttcagcc agcctcccgg gctaaatgcg gtcaggggga
     2701 gttttataaa acgttccttt tgcagtggcc ggcggtccga cacataaaac gaattcgtcc
     2761 gttgatccgc cgcgggccgc attgtttcgg ccttcaggtc ctggccggaa agttctccgg
     2821 aaccggccag cagacacagc ataaagagga aacggccatg cccaaccgat ttcatgatca
     2881 gcttaatcac ggacggatac atcaggtcaa aattcatatc aaataatttg gcggtcaaag
     2941 cgctgtaaat ttatcattat ccgggcagat gacggttgct gcaaatggca gaattggata
     3001 aattatagat tatattacct ttattaaact aataagacaa aaatgcagat aaaattatca
     3061 attaatgaag cctacttttg aaccattggt ctcttctgcc aatacttctt tcctggtcag
     3121 gcattttgag gaaaaatgtt tttccgcccc ttaccatttt catcctgaat atgagctgac
     3181 ctggatcgtg gaaggctccg gaaaacggta tgtgggaacg catatgcacg attatttccc
     3241 gggagacctg gtattactgg gatccaacct gccccattgc tggaaaacag agaactcaaa
     3301 agccggggaa aattccagct cggttgtcgt ccagtttcat ccggacttca tggggaagga
     3361 ttttttttcc aggccggaaa tgaaatatat ccatcgcctg ctcaccaata gccagtccgg
     3421 gatacaattt accggaaata cggagaacct gaaagagaaa atgaaccgca tgctggatga
     3481 aaatcattct ttcagaaaat gggcgctgct gatggatatt ctaaatgaac tggcttcaac
     3541 aaaggaatac agcctgttga gtcaggaggc gccggccccc gaactgtccg tgctggagaa
     3601 agccaggatg aacacctgca tcg
//
LOCUS       DASYCU010000560         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2152384, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000560 DASYCU010000000
VERSION     DASYCU010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152384"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VGM24_11265"
     CDS             complement(<1..521)
                     /locus_tag="VGM24_11265"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEY4156003.1"
                     /translation="MKKIILFFAGSAFLFSACHKAAETGNGSADFATLETQVITDFTN
                     NVALGQYSALTNSAVSLNQAVTTLNSGTTEVNLLAAQASWKNVRGTWEQCEGFLFGPV
                     DDNDYDPNTDTWPTDYNQLDSLLASNNPLETDDIKNLPQSLRGYHPLEYILFGKGGSR
                     QATGLNDRQKKYMV"
     gene            complement(526..960)
                     /locus_tag="VGM24_11270"
     CDS             complement(526..960)
                     /locus_tag="VGM24_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY4156004.1"
                     /translation="MNTQRRKFIKSSCSLCLMAAGGFLFSELAACSPAAQVLKLPVNK
                     NAVRIPLNAFTGSPLLIIRPAGWYFDVAVRKLADNNYEAILLQCTHQQNQLVVSPQGF
                     KCNLHGSQFDEIGNVVKGPAERPLKKFNSTLTADQLIIQLNA"
     gene            complement(997..2244)
                     /locus_tag="VGM24_11275"
     CDS             complement(997..2244)
                     /locus_tag="VGM24_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156005.1"
                     /translation="MKYQALVFFSTIFLFTSISIEAQQFTTKAQQLIRTSEDSLHAGT
                     AGQKTVISGYGSAFYQRDFNKEMSTVTLERAVLFVGHQFSQKISFFSELEIENAKVAG
                     GESNGAEISMEQAFLKFNLNSRQYLVAGLFTPRIGILNENHLPVNFNGVERPLVEQLI
                     IPATWRELGVGFYGSSDKLPLNYSIAVLNGLNAQHFEHGDGIASGRAEGNLATANNLA
                     VTAALQYAWKDFKFQVSGYTGGTVGLSPRGSDSLGLNSGAFGTPIYLGEADLQFSRDG
                     FSAKALGVYISYPDAGKINNRFGKNVADGMYGAYAELGYDWLHRKNKEAQFISFVRLE
                     VLDMNASIPGNGNAIYDGTEKQTHVIAGFSYLPLPNVVIKADVRLLHTGPQNPDLVIN
                     PPPNQLPYRQNNQFLNIGIGYSF"
     gene            2375..3091
                     /locus_tag="VGM24_11280"
     CDS             2375..3091
                     /locus_tag="VGM24_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156006.1"
                     /translation="MKMVKRCLAVILALTAFTGIRAQTADDIINKHFEAIGGKDKIGQ
                     VKSIRMENTVSAMGNDGPSTISAISGKGYRVDTDINGQKIIQVYTDSGGWSVNPFMGA
                     TTPTPLPEEMYKQNRDQIDLSGQLYNYAAKGNKVELAGKENGAYKLKVTNADSVETDY
                     YIDSANYYLVKLTRSVSMMGQTMEVTSTFSDFKKSDFGIVYPYTIEISYGEQFSLTSK
                     VNKLEINPEIDPKIFEMPKS"
     gene            complement(3142..4215)
                     /locus_tag="VGM24_11285"
     CDS             complement(3142..4215)
                     /locus_tag="VGM24_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEY4156007.1"
                     /translation="MLHSVITGSGCYIPPFVQTNQDFAQHVFYTSGGTALHTDPGEVV
                     SKFKKITGIEERRYTTNDLNASDLGFEAAKLAILDSGTDPETLDQLIVAHNFGNVVKD
                     TIQTAAVPSLASLIKNALGIRSPNCVAYDILFGCPGWLQGLIQSDAYLKAGLAKKSLI
                     IGTEALSRVIDMYDRDSMIFSDGAGAVVLEQKEGPDAAPGILGYIAQTYSMEEVDYIN
                     MGASFYPESDPHVLYLKMKGRKVYEFAITQVPAAMKLCLEKSGVPIHELKKIFIHQAN
                     EKMDEAIIKRLYQLYGIDEPPLNIMPMSISWLGNSSVATVPTLFDLVRKNKLPPHTLH
                     PGDVVLFASIGAGMNINAVCYRY"
     gene            complement(4326..4583)
                     /locus_tag="VGM24_11290"
     CDS             complement(4326..4583)
                     /locus_tag="VGM24_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156008.1"
                     /translation="MAKKVKQHSQKSIKKTILKDIEGKLAETLKEYRKKVSEKKFKKS
                     LRKAGKILGNSVTIESIAVVPKKQSKSKKTTTNAAQTELVS"
BASE COUNT         1194 a         1150 c         1190 g         1230 t
ORIGIN      
        1 accatatatt ttttctgccg gtcgttgagg ccggtggcct gcctgctgcc tccttttcca
       61 aacaggatat attccagcgg atgatagccg cgcagactct gcggaagatt tttgatatcg
      121 tccgtctcca gcggattatt gctggcgagc aatgagtcca gttggttata atccgttggc
      181 caggtgtcgg tattggggtc gtagtcattg tcgtctaccg gcccgaaaag aaatccctcg
      241 cactgctccc aggtgccgcg aacatttttc caggatgcct gtgcggccag caggttcact
      301 tcggtcgttc cggagttcag ggtcgtcacc gcctggttga ggctcacggc cgaattggtc
      361 agtgcgctgt attgccccag cgccacatta ttggtgaaat cggtgatcac ctgggtctcg
      421 agcgtggcaa aatcggcgct gccattaccg gtctccgccg ctttgtgaca ggcactgaac
      481 aggaaggcgc taccggcgaa aaataatatt atttttttca tgttattaag cgttgagttg
      541 aatgatgagc tgatccgcag tcagcgtgga attaaatttt ttcagcggcc gttctgcagg
      601 gccttttacc acgtttccga tttcgtcgaa ctggctcccg tgcagattgc atttaaaacc
      661 ctgcgggctc acgacgagct ggttttgctg atgcgtgcac tgcagcagga tcgcctcata
      721 gttattgtca gccagttttc ttacggcaac atcgaaatac catccggcag gccttatgat
      781 cagcagcggg gaaccggtaa acgcattcag cgggatccgt accgcgtttt tgttcaccgg
      841 caatttcagc acctgggcgg ccggactgca ggcggcgagt tcggaaaaaa gaaatccgcc
      901 tgccgccatc aggcagagac tgcaggacga ttttatgaat tttcggcgtt gggtgttcat
      961 gatttccgga tgaatggttg aaatataacg gccatgctaa aacgaatatc cgatcccgat
     1021 attcaggaat tgattgttct gacggtaagg caactggttt ggcggcggat tgatgacgag
     1081 atcagggttc tgcggaccgg tatggagcag gcggacatcc gccttgatca ccacattggg
     1141 caggggcagg tagctgaacc cggcaatcac atgggtttgt ttttccgtac catcgtaaat
     1201 ggcgtttccg ttgccgggaa tggaagcatt catgtccagg acttccagcc tcacaaaact
     1261 gatgaactgg gcttctttgt ttttcctgtg cagccagtcg taccccagtt ctgcataagc
     1321 cccgtacatg ccatcggcaa cattcttccc gaagcggtta ttgatcttgc cggcatcggg
     1381 atagctgata taaacgccga gggcttttgc tgaaaacccg tcccggctaa actgcaggtc
     1441 cgcttctccc aggtaaatgg gtgtgccgaa ggcgccgctg ttcaaaccca ggctatccga
     1501 tccccggggg ctcagtccga cggttccccc ggtatatccc gaaacctgga atttgaaatc
     1561 cttccaggca tattgaaggg ccgcagtcac cgccaggtta ttcgccgtag cgaggttgcc
     1621 ttcggcccgc ccgctggcaa taccatcccc atgctcaaaa tgctgggcgt tcaacccgtt
     1681 taaaacggct atgctgtagt tcaggggaag cttgtcggat gaaccataaa aaccaacccc
     1741 gagttctctc caggtggctg gaatgatcag ctgctcgacc aggggccgct ccactccgtt
     1801 aaaattgacc ggcagatggt tctcgttcag tatcccgata cggggtgtaa ataagcccgc
     1861 aaccaggtat tgcctggaat tgaggttaaa tttcaggaaa gcctgttcca tggaaatttc
     1921 cgccccgttt gattctccac cggctacctt ggcattctcg atctccagtt ccgaaaaaaa
     1981 ggagatcttc tgggaaaact ggtgtcccac aaacaaaacg gccctttcca gggtcaccgt
     2041 agacatttcc ttattgaaat cccgctgata gaaagcgctt ccatagcccg aaatcacagt
     2101 tttctgaccc gccgtgcccg catggaggga atcttcactg gtccggatca attgttgtgc
     2161 tttggttgta aactgttgag cttcaataga aatactggtg aacaggaaga tggtggaaaa
     2221 aaagacgagc gcctgatatt tcatcttgca aaagaacccc ggtgccggca aatggaatta
     2281 cggtttcgga aaaatgattt atacaatccg gaaaaccccg aaaatgtttt cacttcaaat
     2341 aaaaatggga tatttataaa caaaaaacac aaacatgaaa atggtaaaaa gatgcctggc
     2401 agtcatcctc gcgctaaccg catttacagg gatccgggca cagaccgcag atgacattat
     2461 caataagcat tttgaggcga tcggtggcaa ggataagatc ggccaggtaa aatccatccg
     2521 gatggaaaat actgtttcag ccatgggcaa tgatgggccg agcaccattt ctgccataag
     2581 cggtaaagga taccgtgtcg atacggacat caatgggcag aaaatcattc aggtatatac
     2641 cgatagcggc ggatggtcag tgaatccgtt catgggagct accacaccga ctcctctccc
     2701 ggaagaaatg tacaaacaaa acagggatca gattgacctc tccggtcagc tttataatta
     2761 tgccgctaaa ggcaacaagg tcgaactcgc cggaaaagaa aacggggcgt ataaactaaa
     2821 agttaccaac gcagattcgg tggaaacgga ttattatatt gattccgcca actactacct
     2881 ggtcaaatta acacgttccg tatccatgat gggacaaacg atggaagtga ccagcacgtt
     2941 ctccgatttc aagaagagtg atttcggtat tgtatatcca tataccattg agatcagcta
     3001 cggagaacag ttcagcctca cgtccaaagt gaacaagctg gaaataaatc cggagataga
     3061 tccgaaaatt ttcgagatgc cgaaatcctg aaatttaaat taatgcagcg ctggtaaccg
     3121 gggtagcaat tgccccggga atcagtacct gtagcaaacg gcattaatat tcattcccgc
     3181 gccaatggat gcgaacagga ccacgtcgcc aggatgaagg gtatgcggag gcaatttgtt
     3241 tttgcggacg aggtcaaaca gggtggggac ggtagcgaca gaactgttac ccagccagct
     3301 gatgctcatg ggcatgatgt tcaggggagg ttcatcaatc ccgtaaagct ggtagagtct
     3361 ttttattatg gcctcatcca ttttctcatt cgcctggtga atgaatattt ttttcaattc
     3421 atggatcgga acgccgctct tctccaggca tagcttcatg gcggcgggaa cctgcgtgat
     3481 ggcgaactcg tatacttttc gccctttcat tttgagataa aggacatggg ggtcgctttc
     3541 gggataaaag gatgcgccca tatttatata atccacctct tccatggaat aggtctgggc
     3601 aatataaccc aggattccgg gcgccgcatc aggtccttct ttctgctcca gcaccaccgc
     3661 gccggccccg tcactgaaga tcatgctgtc ccggtcatac atatcgatca cccgcgataa
     3721 ggcttccgtt ccgataataa gagatttttt tgccaggccg gcttttaaat acgcatcgga
     3781 ctggatcagt ccctgcagcc agccgggaca cccgaataga atatcataag ccacacagtt
     3841 cggactgcgg atccccagag cattttttat caggcttgcc agcgagggca cggccgccgt
     3901 ctgaatggta tcctttacta cattcccgaa attatgggca acgatcagct gatccagggt
     3961 ctccggatcg gttccgctgt ccaggatagc cagcttggcc gcctcaaaac ctaaatcgga
     4021 agcattcaga tcattcgtgg tatagcggcg ttcttcgatg cccgtgatct ttttaaactt
     4081 gctgaccact tcgccgggat cggtgtgaag agccgttcct ccggaagtat aaaaaacgtg
     4141 ctgggcaaag tcctggtttg tctggacgaa aggcggaata taacagccgg agcctgtaat
     4201 gactgaatga agcataatca atccggacaa cggttaggta atagaaatgc cgatgccgct
     4261 aaggtagaaa aaaaaagcca gcgatgaatg gttcatcacg ggctttccgg ctgaccggtc
     4321 gtgtctcagg aaactaattc ggtttgcgca gcgtttgtcg ttgttttttt ggacttggac
     4381 tgtttctttg gaacaaccgc aatactttct atcgtaacgg agttgccgag aatttttccg
     4441 gctttgcgga gacttttctt gaattttttt tcgctgacct ttttgcgata ctccttgagc
     4501 gtttctgcca gtttgccttc aatatcctta agtattgtct ttttgatgga tttctgacta
     4561 tgctgtttaa ctttttttgc catgactgcc tgtattttaa aaatcaaaga ttgagttcat
     4621 ataaagataa catggataat cctcccgcca atgttaaggt tttaagtttt ccgaaatttg
     4681 aggcggacgg tataattatt tgctaatcaa taattaacaa aggttgaagc aactttattt
     4741 tttattcttc agggaggctt tgga
//
LOCUS       DASYCU010000561         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_120140, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000561 DASYCU010000000
VERSION     DASYCU010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120140"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /gene="ffh"
                     /locus_tag="VGM24_11295"
     CDS             complement(<1..1134)
                     /gene="ffh"
                     /locus_tag="VGM24_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY4156009.1"
                     /translation="MFENLSEKLDSAFKQLKGQGRITELNIAATVKDIRRAMIDADVN
                     YKIAKEFTDTVREKAIGQKVLTAVSPGQLMVKIVKDELATLMGGNESVFQARGNPAVV
                     LIAGLQGSGKTTFSGKLANYLKTQGFSPMLVAADVYRPAAIEQLKILAEQISVDLFTV
                     DGSSDPVAIARQAVNEAKTRNKNVVIVDTAGRLAVDEAMMTEVANIKKAVNPQEILFV
                     VDSMTGQDAVNTARAFNERLDFTGVVLTKLDGDTRGGAALSIKYTVNKPIKFVSSGEK
                     METLDVFYPERMAQRILGMGDITTLVEKAQANFDEEQSRKLEKKIRKNQFDFEDFRVQ
                     LDQIRKMGNIKDLLGMIPGVGKSIKDIDISNDSFKGIEAMIGSM"
     gene            complement(1263..2132)
                     /locus_tag="VGM24_11300"
     CDS             complement(1263..2132)
                     /locus_tag="VGM24_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEY4156010.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVELINPEEEVKLAIRIK
                     QGDQKALDKLTKANLRFVVSVAKQYQNQGLTLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQSLAEQSRIVRLPLNKVGLTNRIQKAYSLLEQQFEREPSPEELAE
                     VLELDIEEVSSTLGIGGRHISVDTPLSEGEDNTLIDVMENPNSESASVNLEYNESLKK
                     DIDRSLQTLTERQKEVICYFFGIGVDHPMSLEDIGDRFSLTRERVRQIKDKAITKLRS
                     NSGAKSLRNYLGI"
     gene            2440..2820
                     /locus_tag="VGM24_11305"
     CDS             2440..2820
                     /locus_tag="VGM24_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HEY4156011.1"
                     /translation="MSKKQQFTLEYSLKCPPSILYEFLSTPAGLQEWFADKVDERDNI
                     FSFSWNGSTTKAEVLESELEKFIRFHWSDSPKEEFFEFRIEKSDITNQTILLIKDFAD
                     KKDIKDQSQLWSYQVKDLFHRLGN"
     gene            2926..>4027
                     /locus_tag="VGM24_11310"
     CDS             2926..>4027
                     /locus_tag="VGM24_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY4156012.1"
                     /translation="MIKKLDKLIVRAFIGPFIATFFVTLFVLVLQFFWLYIDDIVGKG
                     LDFFTIGRFILYVCATLVPLALPLAVLLSSIMTFGNLGETFELVAIKSAGIPLLRFMR
                     PLFITSLVICGIAYGFNNYIIPVAELKMQTLKYDIIVSKPAFDIKEGVFYDKIDGYVI
                     KIGKKDKDGATIHDILIFQHNYNLLQDFATVAQSGKMTISPDKKFLEFNLKNGWNYQI
                     KGPRNSIQTELIRMGFAEYKKVFDLSSFKLNKTDDSAFKNNYQMLNMRQLGTTIDSIE
                     KTTGSYIKQSKATFLSYLRTIPYMDSSWKTRALTLKSDSFQKSLPDSALRPTLERTFT
                     SINNLKSNFDLSSISYENDRKNLRLHLMAWHEK"
BASE COUNT         1015 a         1031 c          853 g         1128 t
ORIGIN      
        1 catcgatccg atcatggctt ctattccttt gaaggaatcg ttgctgatat caatatcctt
       61 tatcgatttg cccacccccg gaatcatgcc cagcaggtcc tttatatttc ccattttacg
      121 gatctgatcc agctgcaccc ggaaatcctc aaaatcaaac tggttctttc tgattttctt
      181 ttccagcttt cttgactgct cctcgtcaaa attggcctgg gccttttcca ccagcgtggt
      241 gatatccccc attcccagga tccgctgtgc catcctttcc gggtaaaaca cgtccagggt
      301 ttccattttc tccccgctgc tgacgaactt aatgggtttg ttgaccgtgt acttgatgct
      361 cagggcggcc cctccccggg tatcgccatc cagcttggtc agtaccaccc cggtaaagtc
      421 aagcctttca ttaaaggccc tggccgtatt aacggcatcc tggccagtca tggaatccac
      481 cacaaacaag atctcctggg gattgaccgc ttttttaata ttcgcgactt ccgtcatcat
      541 agcctcatcc accgccagtc gtccggcagt atccacgata accacgtttt tattacgggt
      601 cttggcctcg ttcaccgcct gcctggcaat cgccaccggg tcactgcttc catcgacggt
      661 aaacaggtcc acactgatct gttcagccaa tattttgagc tgttcgatgg cggccggccg
      721 gtatacgtct gccgctacca gcatcggcga gaacccctgg gttttgagat aattggccag
      781 tttcccgctg aaggtggtct ttccgctacc ctgaagcccg gcgatcagca cgacggccgg
      841 attgcccctg gcctgaaaaa cactctcgtt gcctcccatg agggtggcga gctcgtcctt
      901 cacgattttc accatcagct ggcccggact taccgccgta agcaccttct gcccgatggc
      961 cttttcccgc accgtatcgg taaactcctt agctatctta tagttcacgt cggcatcgat
     1021 cattgcccgg cggatatcct ttaccgttgc cgctatattg agctcggtaa tccggccctg
     1081 tcccttgagc tgcttaaatg cagagtcgag tttctcactt aaattttcaa acatattccg
     1141 gcaatcacta ttgttgagga aaagaaaaaa cgtgaataaa catccgttgt atccacgttt
     1201 ttaagaaagg caaaagtaag aaattgaagt gtaatattat atcaaaccgg ctgattttga
     1261 aattaaatac cgaggtagtt ccggagcgat ttggcccccg aattgctcct cagcttggta
     1321 atggccttgt ccttgatctg ccgcacccgc tcccgggtca ggctgaaccg gtccccgatg
     1381 tcttccaggc tcatgggatg atccacccca atgccaaaaa aatagcagat cacttctttc
     1441 tgacgctcgg taagcgtttg gagagaacgg tcaatatctt tttttaaaga ctcattatac
     1501 tccagattaa ccgaggcact ctccgaattc gggttttcca tcacgtcaat cagggtattg
     1561 tcctcccctt ccgagagcgg cgtatccacg ctgatatgcc tgcccccgat tcccagggtg
     1621 gacgaaactt cttctatatc cagttcaagt acttccgcca gttcttccgg agagggttcc
     1681 cgttcaaatt gctgttccag cagggaatac gctttctgga ttcgattggt cagccccact
     1741 ttattgagcg gaagccgtac gatccgggac tgctcggcca gggactgaag aatgctctgc
     1801 cggatccacc acacggcata ggaaatgaat ttgaaacccc gcgtttcgtc gaaccgctgg
     1861 gcggccttga ttaatccgag attaccttca ttgatcaggt ccgggagggt tagtccctgg
     1921 ttctgatact gtttggcaac ggaaactaca aaacgaagat tggctttggt gagtttgtcg
     1981 agggcctttt ggtcgccctg cttgatgcgg attgcgagtt ttacctcttc ttccgggttg
     2041 atcaattcaa cttttccgat ctcctgcagg tatttttcca agctctgaga ttcacgattg
     2101 gtaatactct tcgtgatctt gagttgacgc atactcatag gcaggtattt tttaagggtt
     2161 taagattcaa agggttcatg atatacttcg gactataaaa acgcacaaat gaaccttttc
     2221 ttatttaaca tttcagaagg attcatttta aattaaccaa tataaccatt tggaagcaaa
     2281 acgccaaacg ggatttggtt taaaggcctg gtactcagtg aattccctat tttttatttc
     2341 atgatatatt cataatcaat gctttcgggg caattaaaaa aaattttaag gcttgaaata
     2401 tttatctata attgcagccc acgaacaaca gcaaccgaga tgagtaagaa acagcagttc
     2461 acattggaat attccctaaa atgccctcct tccattttat atgaattttt gagtaccccg
     2521 gccggcctcc aggagtggtt tgccgataaa gtggatgaac gggataacat tttcagcttt
     2581 tcctggaacg gctcgaccac aaaagcggaa gtgctggaaa gcgagctgga aaaatttatc
     2641 cgttttcact ggtccgattc tcccaaggaa gaattttttg aattccggat cgaaaagtca
     2701 gacatcacca atcaaacgat attgctgatc aaggattttg cagataaaaa ggatatcaag
     2761 gatcagtccc agctctggag ttaccaggtg aaagatcttt ttcaccggct cgggaactga
     2821 tcaatttaac tgcggattat cattccaaca ggctaccggt tcacaccggc tgtgccgaat
     2881 gccttatttt tgcctttccc atttttctaa ccggaaattc ctggcgtgat taagaagctt
     2941 gataaactga tcgttagagc tttcattggc ccttttatcg ccaccttttt tgttaccctg
     3001 ttcgtgctgg tgcttcagtt tttctggtta tatattgacg atatcgtcgg caaggggctg
     3061 gattttttca ccatcggccg gtttatatta tatgtctgtg caacacttgt tccgctggcc
     3121 ctgccgctgg ccgtactgct atcctccatc atgactttcg gaaacctggg cgaaaccttt
     3181 gaactggtcg ccatcaaatc ggcaggtatt ccgctgctcc gcttcatgcg gccccttttt
     3241 attacctccc tcgtcatctg tggcatcgcc tacgggttta acaattatat catcccggtt
     3301 gccgaattaa aaatgcaaac cctgaagtat gacatcattg tttccaagcc ggctttcgat
     3361 ataaaggaag gggtgttcta cgacaaaatt gacggatatg tgatcaaaat cggaaagaaa
     3421 gacaaagacg gagcaaccat ccacgatatt ttaatcttcc agcacaatta taatctgctg
     3481 caggactttg caaccgttgc acaaagtgga aagatgacca tctcgcccga taaaaaattt
     3541 ctggagttta acctgaagaa cggctggaac tatcagataa aaggaccccg caattccatc
     3601 cagaccgaat tgatccgcat gggctttgcc gaatataaaa aagtgtttga cctcagttct
     3661 ttcaagctga acaaaacaga cgacagcgca ttcaaaaaca attatcagat gctgaacatg
     3721 cggcagcttg gaaccaccat tgattccata gaaaaaacga ccggatctta cattaaacaa
     3781 tcaaaggcca catttctctc ctatctgcgt acaatcccct acatggacag ttcctggaaa
     3841 acccgggccc tgacattaaa atcggattcc ttccaaaaga gccttccgga ttcggcttta
     3901 agaccaacgc tggagagaac ctttaccagc attaacaacc tgaaatcgaa tttcgatttg
     3961 tcatccattt catatgagaa tgaccggaaa aatcttcgtt tacacctgat ggcctggcat
     4021 gaaaaga
//
LOCUS       DASYCU010000562         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_264923, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000562 DASYCU010000000
VERSION     DASYCU010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264923"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VGM24_11315"
     CDS             <1..486
                     /locus_tag="VGM24_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY4156013.1"
                     /translation="PGYLTVIIFQGALIFFMVYHGAHPELSLQVPPLGMLSAALLIGG
                     FYPLTQIYQHASDARDGVRTLSAMLGYKGTFIFSAIVYSMAFVVLALFFRISLEIKEL
                     WVFATFMIPVIVYFMYWAGKVWRNPAHAGYAHTMRMNLIASLCTSLGFIVVFLMHCSL
                     R"
     gene            508..1596
                     /locus_tag="VGM24_11320"
     CDS             508..1596
                     /locus_tag="VGM24_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEY4156014.1"
                     /translation="MSQIISIGVSLPRFKHNQQEILDFMSRIYAFNEKDRRKLKFLYN
                     QSGISNRYSVLADYSLPPEKWAFYPATENLEPFPTLEKRMQVFNQYAPALAAESIRNC
                     LENRISPTEITHLITVTCTGLSAPGLDLYLMELLDLPKNIFRTSVNFMGCYAAIHALK
                     LADSICKSEPMARVVIVCTELCTLHFQQEASMDNITSSLLFGDGSAAVLVTGDGYKTE
                     GLRIRGFYAEVNPKGKQEMSWALSSTGFSMTLRSYVPGLIEADFEALLEKALVKNGLT
                     RDAVSDWCIHPGGRKILEVIEKSLSLPKNKLEAGYEVLNEFGNMSSPTILFVLDKLLK
                     KMENKKKHLVFGAAFGPGLTMETFIAGN"
     gene            1623..2312
                     /locus_tag="VGM24_11325"
     CDS             1623..2312
                     /locus_tag="VGM24_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4156015.1"
                     /translation="MNFTLRSYEPELLDRPGIPAADIERNLKELEVINRWLGGHRISL
                     AGFRTLASGSKKITVAEIGCGGGDNLLAIQKKTDYNRTHLRCIGIDLNPDCIRVAQTA
                     AWKEKPELIVSDYRLVGFSSKPDILFASLFCHHFTEEELIDMFRWMRTRAGSGFFIND
                     LHRHRLAYYAIRGLTAVFSRSYLVKNDAPLSVWRGFKRKELETILEKAGIFQYTIRWK
                     WAFRWLIIVPS"
BASE COUNT          573 a          583 c          569 g          603 t
ORIGIN      
        1 cccggttacc ttaccgtgat catttttcag ggagccctga tcttttttat ggtataccac
       61 ggcgcccacc cggaactgag tttacaggtc cctccgctgg ggatgctatc cgctgccctg
      121 ctgatcggag gtttttatcc cctcacccag atctatcagc acgcatcgga tgcccgggac
      181 ggcgtacgca ccctgagcgc catgcttgga tataaaggga cctttatttt ctccgctatt
      241 gtttacagca tggcctttgt cgtacttgcc ctgtttttcc ggatttcact ggaaataaaa
      301 gaattatggg tttttgcaac ctttatgata cccgttatcg tatattttat gtattgggcc
      361 ggtaaggttt ggcgtaatcc ggcccatgcc ggctatgcac atacgatgcg gatgaacctc
      421 attgcttcgc tctgcaccag ccttggcttt attgttgtat tcctgatgca ttgcagcctc
      481 cgctaggttg acataaaata tttattattg agccagatca tttccattgg tgtgtccctc
      541 ccccggttta aacataacca gcaggaaatc ctggatttca tgagccggat atatgccttt
      601 aatgaaaagg atcgccggaa acttaaattc ctttacaatc aaagtggtat ttccaaccgg
      661 tattccgttt tggcagacta cagcctgccc ccggaaaaat gggcctttta tccggccacc
      721 gaaaacctgg aacctttccc aaccctcgaa aaacggatgc aggtatttaa ccaatacgct
      781 cctgctttgg ccgccgaatc catccggaat tgcctggaaa acaggatttc ccctaccgaa
      841 ataacccacc ttataacggt cacctgcacg ggattaagtg cccccggcct ggatctttac
      901 ctgatggaac tgctggatct tccaaaaaat attttcagaa cttctgtaaa tttcatgggc
      961 tgctatgccg caatccatgc ccttaaactg gccgacagca tttgcaaatc cgaaccgatg
     1021 gcccgggtgg tcatcgtatg cacggaactt tgcactctcc attttcagca ggaagccagc
     1081 atggataata tcacttccag tcttttgttc ggcgacggat ctgcagcggt cctcgttacc
     1141 ggagacgggt ataaaacgga ggggctccgg atccggggat tttatgctga agtgaatccc
     1201 aagggaaaac aggagatgag ctgggcatta tcttccacag gtttttcgat gaccctgcgc
     1261 agttatgtcc ccggacttat cgaagcagat tttgaggccc tcctggagaa agcgctcgta
     1321 aaaaacggtc ttacccggga tgccgtttct gactggtgca tccacccggg gggcagaaaa
     1381 atcctggaag tcatagaaaa aagcctttcc ctgcccaaaa ataagctgga agcgggttat
     1441 gaggtgctga acgaatttgg aaatatgtct tcgccgacta tcctttttgt gctggacaaa
     1501 ctgttaaaaa agatggagaa taaaaagaag catttggtat ttggtgcagc atttgggccg
     1561 gggctgacca tggaaacttt tattgcgggt aactgacccg ccgaatcgca accgggcaaa
     1621 aaatgaattt caccttgcgt agctatgaac cggagttgct ggaccggccg ggaattccag
     1681 cagcggatat tgaacgaaac ctcaaagaac tggaagtgat caaccggtgg ctgggcggtc
     1741 accggatttc gctggccgga ttcaggaccc tggcctccgg aagtaagaaa atcacggtgg
     1801 ctgaaatcgg gtgtggcgga ggagacaacc tgctggccat ccagaaaaaa acggactata
     1861 accggacgca tctccgttgt ataggcattg acctgaaccc ggattgtatc cgggtggcgc
     1921 aaaccgctgc ctggaaggaa aaaccggaac tcatagtaag cgactaccgg ctggtgggct
     1981 tttcgtccaa accggatatt ctttttgcct ctttgttttg ccatcatttc acggaagagg
     2041 agctgatcga tatgtttcgc tggatgcgga cccgggccgg gtccggattt ttcataaatg
     2101 atctgcatcg ccatcgtctt gcctattatg cgatccgcgg actgacagct gttttttccc
     2161 ggtcttacct ggtaaaaaat gacgctcccc tttccgtatg gagaggcttt aaaagaaaag
     2221 aactggaaac tatcctggaa aaagcgggaa tttttcaata cacgatcagg tggaaatggg
     2281 cattccgctg gctgataatc gttccttcat gaccaatccc ccgaccat
//
LOCUS       DASYCU010000563         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2730762, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000563 DASYCU010000000
VERSION     DASYCU010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2730762"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VGM24_11330"
     CDS             complement(<1..577)
                     /locus_tag="VGM24_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEY4156016.1"
                     /translation="MLMAKKKSKAWRMILFVMSTAIILMLMVMLWQWWTEKRAGFVHY
                     PEFGISVPMNYSIHGIDVSKYQGSINWQLVKEMRVNDIQISFAFVKATEGLGNEDAMF
                     DRNWRKIKSAGITRGAYHFFLATKSGKAQAENFINSVTLLPGDLPPVLDIEQTYGVPP
                     DKLRERAREWLQTVQAYYGMPPIVYTNVDFYK"
     gene            668..>1587
                     /locus_tag="VGM24_11335"
     CDS             668..>1587
                     /locus_tag="VGM24_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HEY4156017.1"
                     /translation="MPDFNRNDTINLLSKLSLRRTWNGMKVLLSFYASKWTRRSVQWG
                     LPVSISFEPTTSCNLRCPECPSGLRAFTRPVGMLEKEFFRDMIDQLAPDLLYLTFYFQ
                     GEPYLNPAFLDMVEYASAKKIYTATSTNAHYLNDANARRTVESGLDRLIISIDGTTQE
                     VYSQYRIGGQLEKVLAGAAHIVKWKKKLKSKTPFVFFQFLVVRPNEHQMEDARVLAKK
                     TGVDGIRFKTAQVYDYENDPNNLIPRQSSLTRYRKKPDGSFEPKNKLHNHCWRLWHSP
                     VITWDGLVVPCCFDKDAQHRMGDLKKKSFR"
BASE COUNT          396 a          423 c          376 g          392 t
ORIGIN      
        1 ttttataaaa atcaacattg gtgtaaacga tcggcggcat tccataataa gcctggacgg
       61 tttgcagcca ttccctggcc ctttcccgga gcttatccgg gggcacgcca taggtttgtt
      121 cgatatccag cacgggcggc aagtcgccgg gcagcagggt gacggagttg atgaaatttt
      181 ccgcctgggc cttgccgctt ttggtggcca ggaaaaaatg ataggcgccc cgggtgattc
      241 cggcactttt aatttttcgc cagttgcggt cgaacatggc atcttcgttt cccagtccct
      301 ccgtggcctt gacaaaagca aaactgatct gaatatcatt cacgcgcatt tctttcacga
      361 gttgccagtt gatggatccc tggtacttgg atacatcaat gccgtgaatg gaataattca
      421 tcgggaccga gatcccgaat tcagggtaat gaacaaaacc ggcccttttt tctgtccacc
      481 attgccagag cataaccatc agcatcagga ttatagcagt ggacataaca aacaggatca
      541 ttctccaggc cttactcttc tttttggcca tcagcatatt taaacgattg caaatataaa
      601 ctcccgggaa taacccggcg gcctctattc gttctttccg gtttttaaaa ctaattttat
      661 accgattatg cccgatttta accggaacga taccataaac ctgttatcca aactgagcct
      721 gcgccggacc tggaatggca tgaaagtgct tttgagcttt tatgccagca aatggacccg
      781 ccggtccgtg caatggggat tacctgtttc catctctttc gaacccacca cttcctgcaa
      841 cctccgctgt ccggaatgcc ccagcggcct gcgggctttc acccggcccg tgggtatgct
      901 tgaaaaagaa tttttccggg atatgatcga tcagctggcc cctgacctgc tttaccttac
      961 tttttatttt cagggagaac cctatttaaa tccggctttt ctggatatgg ttgaatacgc
     1021 ctcggcgaaa aagatttata ccgccacttc caccaatgcg cattatctga acgatgcaaa
     1081 tgcacgccgg accgtggaaa gcggcctgga tcgcctgatc atctccattg acggcaccac
     1141 gcaggaggtg tacagccaat accgcatcgg aggtcaattg gagaaagtgc tggccggagc
     1201 cgcccatatc gtgaaatgga agaaaaagct caaaagcaaa acgcccttcg tttttttcca
     1261 gttcctggtg gtccgcccca acgagcacca gatggaggat gcgcgcgttt tggccaaaaa
     1321 gaccggagtg gatggtatcc ggtttaaaac cgcccaggta tatgactacg aaaatgatcc
     1381 caacaacctg atcccccgcc agagcagtct gacccgttac cggaaaaaac cggacggaag
     1441 cttcgaaccg aaaaataagt tgcacaacca ttgctggcgc ctctggcatt cccccgtaat
     1501 cacctgggac gggctggtgg ttccctgctg ttttgacaag gatgcacagc atcgcatggg
     1561 tgaccttaaa aagaaatcat tccggga
//
LOCUS       DASYCU010000564         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4761680, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000564 DASYCU010000000
VERSION     DASYCU010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4761680"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VGM24_11340"
     CDS             complement(<1..1388)
                     /locus_tag="VGM24_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY4156018.1"
                     /translation="MEILHVSAECYPVAKAGGLGDVVGALPRYQQEAGHTAKVVMPMY
                     RTKFLNSHEWDVVHKGYTNLGNWWFIYTIIKEKHNSLGFDLYLVDINGLLDRQKIYGY
                     DDDPARFTAFQIAVVDWLAAWKHRPTLIHVHDHQAALIPFMIKHCYRYQQLRDIPTVL
                     TIHNAEYQGWLGWDKSYYLPDWDTWKSGLLEWNNSINSLACGIRCAWKVTTVSWTYLE
                     ELRWSANGLEALFEYERGKCYGILNGIDTKTWDPETDTYLENHFNIKTRSEGKEVNKK
                     LLCERFSLDEKKPLIAFIGRLVGEKGADLLPQAVGDSFYYIGRKMSFLVLGSGSAAIE
                     QNLERVRTLTGGDCSIYIGYDEALSHMIYAGADFILMPSRVEPCGLNQMYAMRYGTVP
                     VVRNTGGLRDTVKDFGDPDGYGIRFNNAVVGDITQAVWRAVQLYEDPVMMDELRRRMM
                     RLDFSWETSMNH"
     gene            1533..>2474
                     /locus_tag="VGM24_11345"
     CDS             1533..>2474
                     /locus_tag="VGM24_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4156019.1"
                     /translation="MSQPKQPTNGGALATLVIVFFFWGFIAASNSIFIPFCRTHFHLN
                     QFQSQLIGSAFYGAYFIGSLILYLLSSLVGYDILNKIGYKRGIVLGLLISVAGALLII
                     PSANLNSFPAILGSFFVVALGFSLQQTAAQPFAIALGDPATGTHRLNLAGGINSLGTT
                     LGPIIVSYFLFGTPSAANESVTVTNINTLYLIVAAVFLAVALFFAFSKLPEGKDDAAF
                     EGSKKASMSLITLTVLMLVIILIGQFTKIGKLELTGITILLVFAVLFLSYSMASKNSD
                     GWGAMKYPQLILGMIGIFVFVGVEVTIDNNFGALLKSP"
BASE COUNT          588 a          633 c          547 g          706 t
ORIGIN      
        1 taatgattca tactggtttc ccagctaaaa tccagccgca tcattctcct tcgcaattca
       61 tccatcataa cggggtcttc atacaactgg acagcccgcc atacggcctg ggtgatatct
      121 ccgacgactg cattattaaa acggatcccg taaccgtccg gatccccaaa gtccttaacc
      181 gtatctctta atcctccggt attccggacc acggggacgg ttccatagcg catcgcgtac
      241 atttggttta atccgcaggg ctccacgcgc gaaggcatca ggataaaatc cgccccggca
      301 taaatcatat ggctgagtgc ctcgtcataa ccgatataga tgctgcagtc ccccccggtg
      361 agtgttctga cccgttccag attttgttca atagccgccg agccgctgcc cagcaccagg
      421 aagctcattt tcctgccgat ataatagaat gaatctccga cagcctgtgg caacagatcc
      481 gcgcccttct ctcccaccag tctcccgata aaggcaatca gcggtttttt ttcgtccagg
      541 gagaaccgct cgcatagcaa tttcttattg acctcttttc cttccgaccg ggtcttaata
      601 ttgaaatggt tttccaggta agtatctgtt tccggatccc atgttttggt atctattccg
      661 ttcagtatcc cgtaacactt tcctctttca tattcaaaaa gcgcttccag cccgttggcc
      721 gaccaccgga gctcttccag gtaagtccag cttacggtgg tcactttcca cgcgcagcga
      781 atgccgcagg ccagggagtt aatggaatta ttccattcca gcaatcccga tttccaggta
      841 tcccagtccg gcagataata acttttatcc catcccagcc atccctggta ttccgcattg
      901 tggatggtca gcacggttgg gatatccctt aactgctgat accggtaaca gtgttttatc
      961 atgaagggga tcagggcagc ctggtggtcg tgtacgtgaa tgagggtggg acgatgtttc
     1021 caggcagcga gccagtcgac cacggcaatt tgaaatgcag taaaccgggc ggggtcgtca
     1081 tcatatccgt aaatcttttg ccggtcgagc aatccgttaa tatccaccag gtagagatca
     1141 aaacccaggg aattgtgctt ttcctttatg atcgtgtaaa taaaccacca gtttcccaga
     1201 ttggtatacc ctttatgaac cacatcccac tcatgggaat tcagaaattt tgtccggtac
     1261 atgggcataa ccactttggc ggtatgtcct gcctcctgct ggtaccgggg aagggcacca
     1321 acaacatctc cgagcccccc cgctttggca accggataac attctgctga aacatgtaat
     1381 atttccattg ggatggcaaa tctggttatg gggagagatc gaattatatt caatttagga
     1441 aggatttgct gattctgacg agatatgaaa aaatttttca tccagcaaaa acttaactac
     1501 tttcaacctt atttataaat tccacccagc ttatgagtca acctaaacag ccaacgaatg
     1561 gcggagcctt agctaccttg gttatagtgt tttttttctg gggatttatt gctgcatcca
     1621 atagcatttt tattcctttc tgcagaactc attttcacct gaatcaattt cagtcccagc
     1681 tgatcggatc ggcattttac ggggcatatt tcattggctc gcttatatta tacctgcttt
     1741 ccagtttggt tggatatgat atattaaaca agataggata taaaagaggc atcgtactcg
     1801 gcttgctgat ttcggttgcc ggcgctttat tgattatccc ttctgccaac ctcaattcgt
     1861 tccctgccat cctcggttct ttctttgtgg tagccctggg attttcactt cagcagactg
     1921 ccgcccaacc cttcgctatc gccctgggag atccggccac gggcacacac cggctaaacc
     1981 ttgccggggg gattaattcg ctcggcacca cgctgggacc gatcattgtc agctatttcc
     2041 tttttggaac accgtctgct gccaatgaaa gtgtcactgt taccaatata aataccttat
     2101 acctgatcgt ggctgcggta tttctggccg ttgctttgtt ttttgcgttt tcaaaactcc
     2161 cggaagggaa agacgatgcg gctttcgaag gatcgaagaa ggcctcgatg tcactgatta
     2221 ccttaacggt gctgatgctc gtgatcatac tgatcggaca attcaccaag atcggaaagc
     2281 tggaactgac cggcatcacg atactcctgg tatttgccgt tttattcctg tcttatagca
     2341 tggcatcaaa aaacagtgat ggctggggtg ctatgaaata tccgcaattg attttgggca
     2401 tgattgggat ctttgtattc gtaggggtcg aagtgacgat cgataataat tttggcgctt
     2461 tgttaaaatc accg
//
LOCUS       DASYCU010000565         9200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_121755, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000565 DASYCU010000000
VERSION     DASYCU010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121755"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VGM24_11350"
     CDS             <1..322
                     /locus_tag="VGM24_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156020.1"
                     /translation="FLGSYKQLMKLFVAGLPYDFDDTDLKEMFELYGEVSSAKVAIDR
                     ETRKSRGFGFLEMTVALEAKEAIDALHGARLRGGKELVVREAEEKPRDSRDNRSPGGF
                     RPRR"
     gene            complement(334..1125)
                     /locus_tag="VGM24_11355"
     CDS             complement(334..1125)
                     /locus_tag="VGM24_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4156021.1"
                     /translation="MDLQLKDKVIIVTGGAKGIGEGISRVLAAEGARVAIVGRKEEDN
                     RNLVREIRESGGEAFSFQAELTRPEVCEKTINEIISSLGRINGLVNNAGLNDGVGLET
                     GNYDRFMISMHNNIVHYYLMAHFALPELIKSKGAILNISSKSAETGQGNTSAYAAANG
                     GRNALTREWAVELLKYQVRVNAIVVAECWTPQYANWIRKFPDSEKRLNEIISRIPLGN
                     RMTTIGEIGNMAAFLISERSSHTTGQLIHVDGGYVHLDRALKNEG"
     gene            complement(1150..1491)
                     /locus_tag="VGM24_11360"
     CDS             complement(1150..1491)
                     /locus_tag="VGM24_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY4156022.1"
                     /translation="MPAETKRYCLALDLKNDHRLIEAYKSHHQKVWPEIVKSIRDADI
                     VSMEIYQVENRLFMIMETGPGFSFEKKANDDLQNQKVREWEQLMETYQQLLPGTSKNE
                     KWRLMERIFLL"
     gene            complement(1496..2359)
                     /locus_tag="VGM24_11365"
     CDS             complement(1496..2359)
                     /locus_tag="VGM24_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4156023.1"
                     /translation="MKLIRFLQDGKVLPGAVIDAESYDLSAFGADYDEGFFESGGLAR
                     LRTFIDANRNKLEKLSEPVHLQSPVSRPSKIVCVGLNYADHAKETLAEKPKEPVLFMK
                     STTSLAGPFDPIVIPKNSKKTDWEVELAVVMARKASYVEEKEAADYIAGYCVHNDVSE
                     REFQIERGGTWDKGKGCDSFAPLGPWLVTADEIADTNNLKLWLKLNGKMMQNGTTAHL
                     IFKIPFLISYISQFMTLLPGDIISTGTPAGVGLGMNPPVYLKPGDVVELGIDGLGTAR
                     QEVRAYADAKK"
     gene            complement(2378..3208)
                     /locus_tag="VGM24_11370"
     CDS             complement(2378..3208)
                     /locus_tag="VGM24_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY4156024.1"
                     /translation="MQKIDSHVHFWDYDPVKNSWITEDMAVIRRSFLPQDLEAILKKQ
                     GMDGCVLVQVNQAEEENDFFLKLASEHNFVRGMVGWVDLCNGQVMDRLAHYRVSGKIR
                     GFRHILQDETDRAYMLRPDFKRGIGSLREFGFTYDLLIRPDQLPFAAQLVKSFPEQPF
                     IIDHLAKPAIRDQEIADWKKGIYRISRMENAFCKLSGMLTEADWKSWTNETFSPYIDV
                     IVECFGVDRILFGSDWPVCLLAGSYGNVLKIVEEYFSSFTETEKEKIFGGNATAFYGL
                     "
     gene            complement(3277..4737)
                     /locus_tag="VGM24_11375"
     CDS             complement(3277..4737)
                     /locus_tag="VGM24_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY4156025.1"
                     /translation="MRRIFLLIGFGFFFVAPGLKARAQAKFQANWESLKKYQTPDWFR
                     DAKFGIFIHWGVYSVPAFGSEWYPRQMYQQGSEEFKHQVEHFGPQDKSGYKDFIPLFK
                     AEHFNADAWAELFKKAGARYVVPVAEHHDGFAMYKTALSRWNAAEMGPHKDIIGLLSA
                     AVRKQGLIFGLSSHRIEHWWFMNGGRRFNSDVNDPAYADFYGPAREESETPSPEFMND
                     WLLRCTELVDKYKPRLFWFDWWIEQPAMDPYRKSFAAFYYDKGLEWNEGVVINYKNKA
                     FPDGTAVLDIERGKLGGIRTLPWQTDDAIGNNSWGYTTTNTFKTAQYVITNLIDIVSK
                     NGNLLLNIGPRPDGTITEEETTVLLKTGKWLDVNGKAIYGTRPWKIYGEGPTESASGS
                     FADQKVPFNAKDIRFTTHSDTLYATCLGLPNETTRIKSLGLNANNGLIANLELVGSGK
                     KIKWVQHKDALEISPSADYPSEDAVVYEIIFKKAGS"
     gene            4989..5543
                     /locus_tag="VGM24_11380"
     CDS             4989..5543
                     /locus_tag="VGM24_11380"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4156026.1"
                     /translation="MDIAELIREAKLGSAAAQKCLFDLLADKMQMVCRRYVKNRENAE
                     EIMLDGFYKIFRAMNAFHYEGEAAFYGWVKKIMVNECLMFLRKKNVFSLVTESAAEEI
                     IPEEEVLGQLSASEIFSLIVQLPVGYRTVFNLYAIEGLNHREVADLLNISEGTSKSQL
                     SKARSLLQKMLMQNGIEYVRRKTP"
     gene            5521..6255
                     /locus_tag="VGM24_11385"
     CDS             5521..6255
                     /locus_tag="VGM24_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156027.1"
                     /translation="MSDEKHPNPRSAAGKLDGLDFLPDETRLDKTLAWEKLYSRIRQK
                     QDRKTGGWYRLVAASVLCALLLYGITRPHTGRDLAKHNTKNRGTIIPALPQQPARKSN
                     AGGAESLALKTNKSVQGTLLPHSHNNRARLFTRDIHPAVQDFTPGTAIMAPKSDLPVS
                     EAAALPVDAGTTEKVVPPIAKRKLRVVHMNELDEPMEADDNLVKNSGRHGFRLKWIGQ
                     TSALNTFQATGQTYNELIKINLPSPN"
     gene            6262..7377
                     /locus_tag="VGM24_11390"
     CDS             6262..7377
                     /locus_tag="VGM24_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156028.1"
                     /translation="MKRIILLMMLVTSIKESLFAQENPTVSNTDKKFELSSDKFTRRF
                     LIDLGKGNKLQIELKDMADLDRLKNMDSILRVFLQEIEPLRDSLSDELSSKRIDYTMD
                     SMGRKEIRIQQFRPKGSNFLINKGDLASLKLEQDTVNFIGTVHFIAKYTLRKAFPDQR
                     KYQVSFFVNQLNELPSILAAGLNDKILSLQQNVNSGWKPGQDGRLELIKDPRISAKLP
                     KGYFTGHGDYLSPDISVNVQNYKSYFVPSVSLGIRMVLSNGSYKREIGLLWEPHFLFQ
                     NGQGRLKTFRNDFLSLTFGQGPIKDNDPLKESSLQVILSAGYLVRRQGDYFDKHTIRL
                     GFGRLSLFEGKTKIEPMIFFHDFFREVTPGIRWIQSI"
     gene            complement(7422..7781)
                     /locus_tag="VGM24_11395"
     CDS             complement(7422..7781)
                     /locus_tag="VGM24_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4156029.1"
                     /translation="MTTKEIATRLRDLCAEGKFEMAQKELFSQDAVSIEPEASPGFDK
                     LTKGLPAILEKGHTFEKMVEAVHGIKISDPLVAGNSFALVMDMDTTMKGHGRSKMSEI
                     CVYVVKDGKIVKEEFFM"
     gene            complement(8000..8395)
                     /locus_tag="VGM24_11400"
     CDS             complement(8000..8395)
                     /locus_tag="VGM24_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156030.1"
                     /translation="MKRRMLLLAFSGFMIMAGKAQFANTQWKGEMGIPQGEGQPLEAT
                     EAVWDFKPDTVIVHFPGGGQDEVMTYRVTGDILTLIKVSGSSECDPGSSGKYRYRIQG
                     DQFFIKVSEDSCAGRSAIDFSKPFERVKD"
     gene            8663..>9200
                     /locus_tag="VGM24_11405"
     CDS             8663..>9200
                     /locus_tag="VGM24_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156031.1"
                     /translation="MSTSQNLETLKDIKRMMERSSRFISLSGWSGITAGICALAGAWI
                     ASNRISTYYRNEYSTSPACPSCLKSDLLTIGSLVFIAAFASAMLFTFLKSKKEGVAIW
                     GNAARRLLWNTLLPMAAGGFLLWRMIELKQYEFIASGSLIFYGLALVNGSKYTMGEVR
                     WLGYGQIVLGIINLWAVHS"
BASE COUNT         2310 a         2412 c         2068 g         2410 t
ORIGIN      
        1 atttttggga tcttataaac agctcatgaa actttttgtt gccggtctgc cctatgactt
       61 tgacgatacc gatttaaaag aaatgtttga actctatgga gaagtgagtt ctgcgaaagt
      121 tgccattgac cgcgaaaccc ggaaaagcag gggtttcgga ttcctcgaaa tgacggtggc
      181 cctggaagcc aaggaagcga tcgacgcctt gcacggagcc cggctccgcg gaggaaagga
      241 actggttgta cgcgaggctg aagaaaaacc ccgcgatagc cgcgataacc gcagcccggg
      301 cggattcaga cccaggcgat gatccgcctt tagttatccc tcatttttca atgcccggtc
      361 caggtgtaca tatccaccgt ccacatggat caattgaccg gtcgtatgac tggatctttc
      421 tgaaatcagg aaggccgcca tattcccaat ttccccgatg gtcgtcatcc ggtttcccag
      481 cggaattctg gaaatgatct cattcaggcg tttttcactg tccgggaatt tccggatcca
      541 gttggcgtac tgcggagtcc agcattcggc aacgacaatg gcatttaccc gcacctggta
      601 cttcaataat tcaacggccc attcccgggt cagcgcattc cggcctccgt tggcagcggc
      661 ataggccgag gtattcccct gtccggtctc cgccgatttg gaactgatat tcaggatggc
      721 ccctttcgat ttgatcagct ccggcaaagc gaagtgcgcc attaaataat agtggacgat
      781 attattgtgc atgctgatca taaaacgatc ataattcccg gtttccagcc ccacgccgtc
      841 attgagaccc gcattgttga ccagcccgtt tatcctgccc agggaggaaa taatttcgtt
      901 gattgttttt tcgcacactt ccggtctcgt caattcggcc tgaaaagaaa aggcttcccc
      961 cccggattcc cgaatttctc tgaccaggtt acggttatcc tcttcctttc ttcccacaat
     1021 ggccacccgg gcgccttctg cggccaaaac ccgggaaata ccctcgccga tgccctttgc
     1081 tcccccggtt acgataatta ccttatcctt taactgaaga tccatcctta ggattttaat
     1141 tgttattttt cacaataaaa agatcctttc cataagcctc catttttcat ttttggatgt
     1201 tcccggcagc aactgctgat aggtttccat caactgttcc cattcccgga ccttttgatt
     1261 ctggagatca tcgttcgctt ttttttcaaa tgaaaacccc ggtccggtct ccatgatcat
     1321 gaacagacga ttttcaacct ggtagatctc catgcttaca atatccgcat cacgaatact
     1381 ctttacgatt tccggccaca ctttttggtg atgggattta tacgcttcga ttaaccggtg
     1441 atcattcttc agatccaggg ctaaacagta tctctttgtt tcggctggca tggaattatt
     1501 ttttggcatc cgcataggcc cttacttcct gccgggccgt ccccaggccg tcaatgccca
     1561 gttcaaccac gtctcccggt ttcagataaa ccggggggtt cattcccagg ccgacgccgg
     1621 ccggagtacc ggttgaaata atatctcccg gcagcagggt cataaactga ctgatataag
     1681 aaataaggaa aggaatttta aaaatgaggt gggccgttgt gccattctgc atcatcttcc
     1741 cattcagctt cagccatagt ttaaggttgt ttgtatcggc tatctcgtct gcggtgacca
     1801 gccagggtcc cagcggggca aagctatcac agcccttgcc tttgtcccag gtaccgcctc
     1861 tctcaatctg gaattcccgc tcgctgacat cgttatgcac gcaatacccc gcaatataat
     1921 ccgctgcctc tttttcttca acataggagg cctttctggc catgaccacc gccagctcca
     1981 cttcccagtc cgtttttttc gaattcttcg gaatgacgat cggatcaaag ggaccggcca
     2041 gcgaggtggt tgacttcata aataaaacgg gctccttcgg cttctccgcc agggtttctt
     2101 tcgcatgatc tgcatagttg agtccgacac atacaatctt ggaaggccgc gaaaccggag
     2161 attgcaagtg aaccggttcg ctcaattttt ctagtttatt ccggttggca tcaataaatg
     2221 ttctcagccg ggccaacccg cccgattcaa aaaacccttc atcataatcc gctccaaaag
     2281 cggacagatc atacgattcc gcatcgatca ccgcaccggg caatactttt ccatcctgta
     2341 aaaatctgat cagtttcata tttcgttttt ataccagtta caatccataa aaagccgtgg
     2401 cattcccgcc aaaaattttt tctttctccg tttctgtaaa ggatgaaaaa tattcctcaa
     2461 cgatcttcaa tacatttcca taagatccgg ccagtaaaca tacaggccaa tccgaaccga
     2521 ataaaatccg gtccacgcca aagcattcca caatcacatc aatatatggg ctgaaggtct
     2581 catttgtcca gcttttccaa tccgcttccg tcagcatccc ggacaatttg caaaacgcat
     2641 tttccatccg ggaaatacgg tatatcccct ttttccaatc cgctatttcc tggtcccgga
     2701 tggccggctt ggccaggtga tcaatgataa atggctgctc cggaaatgat ttcaccagtt
     2761 gggccgcaaa gggcagctga tccgggcgga tcagcagatc ataggtgaat ccaaattcac
     2821 gaagtgatcc gattccccgc ttgaaatccg gcctcagcat gtacgcccgg tccgtttcat
     2881 cctgcagaat atgccggaat ccccggatct tcccggatac ccggtagtgc gccagccggt
     2941 ccattacctg cccgttacaa agatcaaccc aacccaccat tcccctgacg aagttatgtt
     3001 cggatgcgag ttttaaaaaa aaatcatttt cctcttccgc ctgattcacc tgcaccagta
     3061 cgcagccatc cattccctgt tttttcagga ttgcctccag atcctgcgga agaaagcttc
     3121 ttcgtataac agccatatct tccgtgatcc aggaattttt cacgggatca taatcccaaa
     3181 aatgcacatg gctgtcaatt ttttgcatat aaattataaa agaaccggct gccggaagga
     3241 ggcagcctgc cggcggcgcc ggcagatctt ccgatattat gatcctgcct ttttaaaaat
     3301 aatctcataa acgacggcgt cttccgaagg ataatcagca gaaggactga tctccaaagc
     3361 atctttatgc tggacccatt ttattttctt tccgctgccc accaattcca ggttcgcgat
     3421 caggccatta ttggcattta aacccaggga ttttatccgg gttgtttcgt ttggcaatcc
     3481 caggcaggtg gcgtacaggg tatctgaatg cgtggtaaaa cggatgtctt tggcattaaa
     3541 aggaactttc tgatcggcaa aagaaccgct ggccgattcc gtcgggcctt ccccataaat
     3601 tttccagggc cttgtgccat aaatggcctt cccgtttaca tccagccatt tgccggtttt
     3661 caataaaacc gtcgtttctt cttccgttat cgttccatcc ggacgggggc ctatattaag
     3721 caggagattc ccgttcttac ttacaatatc aatcaggttc gtaattacat attgagccgt
     3781 tttaaaggta ttggtggtag tatatcccca ggaattattg ccgatggcat catcggtctg
     3841 ccagggcagg gtccggatgc cgcccagttt tcccctttca atatccagaa cggccgtacc
     3901 atccgggaac gctttgtttt tgtaattgat cactacgcct tcattccatt ccagaccctt
     3961 gtcataataa aaagcggcaa atgatttccg gtagggatcc atggcgggct gttctatcca
     4021 ccagtcgaac cagaaaagcc ggggtttata tttatccacc agctcggtgc atctgagcag
     4081 ccagtcgttc atgaactccg gggagggggt ttcactttcc tccctggccg ggccgtaaaa
     4141 atccgcatat gccggatcat tcacatccga attgaatctt cttccgccat tcataaacca
     4201 ccagtgttct atgcgatggg aggaaagccc gaaaatcaat ccctgctttc gaacggctgc
     4261 ggataataaa ccgataatat ctttgtgcgg acccatttcc gctgcattcc acctggatag
     4321 agccgtttta tacatggcaa acccgtcgtg gtgttctgct acgggcacca cataccgcgc
     4381 gcccgctttt ttaaataatt ccgcccaggc atccgcgtta aaatgttccg ctttaaaaag
     4441 cgggataaaa tccttatatc cggatttgtc ctggggccca aagtgctcca cctggtgttt
     4501 gaattcttct gatccctgct ggtacatttg acggggatac cattcgcttc caaaagccgg
     4561 aaccgaataa acgccccaat gaataaatat gccaaacttg gcgtcccgaa accaatccgg
     4621 tgtctgatat ttttttaaag attcccagtt tgcctggaat tttgcctgag cacgggcctt
     4681 gaggccggga gcaacaaaga agaagccaaa gcctataagt aaaaatattc tcctcatatg
     4741 gatcggttaa aaatttccac cggctcttac ccgccctccg gcataccttc tttaccagca
     4801 ttaaatctaa tactaatttt acagatcttt tggccggcag gacggacccg cttgccgatt
     4861 tgcggtttta tccgggtcgg cagaggaacc ttgtcccgct gaatacttta acaaagcccg
     4921 cctggccggg caaccgtttt ttcacgaacc gcattcatac ctttagaagg accaatctgc
     4981 ccgaatagat ggatatagcc gaactgatca gagaagcaaa actggggagc gcagcagcgc
     5041 agaaatgcct gtttgacctg ctggcagaca aaatgcagat ggtctgccgc agatatgtga
     5101 aaaacagaga gaatgccgag gagatcatgc tcgatgggtt ttacaaaatc ttccgggcta
     5161 tgaatgcctt tcattatgag ggagaagccg ctttttatgg ctgggtgaaa aagatcatgg
     5221 taaatgaatg cctgatgttc ctccggaaaa aaaatgtttt ttccctggtt actgagtctg
     5281 cggcagagga gatcatcccg gaggaagaag tgctgggtca gctatctgct tcggaaattt
     5341 tttccctgat cgtgcaactt ccggttggct accggacggt ctttaacctt tacgccattg
     5401 aagggctgaa tcaccgagag gtggcggacc tgctgaatat ctccgaaggt acttccaagt
     5461 cgcagctgag caaagccaga tctcttttac aaaaaatgct aatgcaaaac ggaatcgaat
     5521 atgtcagacg aaaaacaccc taacccgcgc agcgcagccg ggaaactgga cgggctggac
     5581 tttcttcccg atgaaacgcg cctggataaa accctggcct gggaaaagct ctattcccgg
     5641 atacggcaaa agcaggaccg gaaaacagga ggctggtacc ggcttgtggc agcgtctgtt
     5701 ctgtgtgccc tgctcctgta tgggatcacc cgtccgcata ccggcaggga cctggccaag
     5761 cacaatacga aaaatagagg aacgatcatc cccgccctgc cccaacaacc ggcccggaaa
     5821 tcaaatgcgg gtggagcgga gagccttgct ttgaagacca ataagtccgt ccagggtacc
     5881 cttctgcccc attcgcataa taaccgggcc cggttattta cccgcgatat ccatccggcc
     5941 gttcaggatt ttacccccgg taccgctatc atggccccga agagcgatct tcccgtttca
     6001 gaagcagcgg ccttaccggt ggacgccggc acaacggaaa aggtcgttcc gcccatagca
     6061 aaaaggaaac tccgggtcgt gcatatgaat gagctggacg aaccgatgga ggcagacgat
     6121 aatctggtaa aaaattccgg cagacacgga ttccgtctta aatggatcgg gcaaaccagt
     6181 gcattgaata ctttccaggc aaccggccag acctataatg aactgataaa aatcaatctt
     6241 ccttccccaa attaaaaaaa aatgaaaaga atcattcttt tgatgatgct tgtgacaagc
     6301 atcaaagaat ccctgtttgc ccaggaaaac ccaaccgtat ccaacacgga taaaaaattt
     6361 gagctttctt ccgataagtt cacccgccgc tttctcattg atctggggaa aggaaataaa
     6421 cttcagatag aattgaagga tatggccgat ctggacaggc ttaagaatat ggattccatc
     6481 ctccgggtct tccttcagga aatagaacct ctgagagatt ccctgtcgga tgaattatct
     6541 tccaagagaa tcgattatac catggattcc atgggaagaa aagagatccg gatccagcag
     6601 tttagaccca aaggctcgaa ttttttaatc aataagggcg acctggcctc gttgaaactg
     6661 gagcaggaca cggttaattt tatcggaacc gttcacttca tagccaaata caccctgcgg
     6721 aaggcatttc cggaccagcg taaataccag gtgagttttt ttgttaatca gctgaatgag
     6781 cttccctcca tattagccgc gggcctgaat gataaaatac tcagtttaca gcaaaatgtc
     6841 aattccggat ggaagccggg tcaggacggc cgtcttgaac tgataaaaga tccccgcatt
     6901 tccgccaaac ttcccaaagg atatttcacg gggcacgggg attatctcag tccggatatt
     6961 tcggtgaacg tccagaacta caagagttat tttgtcccct ctgtcagcct gggtatccgg
     7021 atggtcttaa gcaatggctc atataagcgg gagatcggac tgctctggga accccatttc
     7081 ctttttcaaa acggacaagg caggctgaaa acattcagga acgatttttt gagcctgacc
     7141 tttggccagg gtccgatcaa ggataatgat cccctgaaag aatcttccct gcaggtgatc
     7201 ctttccgccg gttacctggt ccggagacag ggcgattatt ttgataagca taccatccgt
     7261 ttggggttcg gacgattgtc tctgtttgaa gggaaaacga aaatagaacc aatgattttc
     7321 ttccatgatt ttttcaggga agtgacgccg ggaataaggt ggattcagag tatctgaagt
     7381 aaaaagtaag aaagggcgcc cctttcttac tttttattgg ctcacataaa gaattcttcc
     7441 ttaacgatct ttccgtcttt aaccacgtat acgcagatct ccgacatctt tgaccggccg
     7501 tgccccttca tggtggtatc catatccatg accagggcga acgaattacc ggccaccagc
     7561 ggatcagaga ttttgattcc atgcacggct tccaccattt tctcgaaagt atggcctttc
     7621 tccaagatag ccggaaggcc tttcgtcagc ttgtcaaaac cgggactggc ctccggctca
     7681 atactcacgg catcctggga gaataattcc ttttgtgcca tttcaaactt tccttccgcg
     7741 caaaggtcac gcaggcgggt tgcaatttct ttcgttgtca taatgatttg tttttgaaat
     7801 gaataatctg aaccggttca tttgactacc agttcatatt gctaaaataa ttagtttttt
     7861 atttctaaaa aaattgactt ttagcctccg tccgggaata taagagaagg ccgggtttat
     7921 aatcccgtca aatatcgaac cagtaggcca aagacggatc cggctttaac ccggagatgc
     7981 ggagcatcgg gcggggacgc tagtccttta cgcgctcaaa aggtttcgaa aaatcgatcg
     8041 ccgaccgtcc tgcacaacta tcctcggaaa cctttatgaa aaactgatcg ccctggatcc
     8101 tgtaccggta tttgccggaa ctgcccggat cacactcgct tgatccgctt acttttataa
     8161 gggtgaggat gtctccggta acgcggtaag tcattacctc gtcctgcccg ccaccgggaa
     8221 aatggacgat aacggtatcc ggcttaaaat cccataccgc ttcggttgct tccaggggtt
     8281 ggccctctcc ctgcgggatt cccatttcac ccttccattg ggtattggcg aactgggcct
     8341 taccggccat tatcataaac ccggaaaacg ccagtaacag cattcttctt ttcataagcc
     8401 tgttttttga tatttaagaa ttttaacaaa cattctgaat taaatatatt ccattccccg
     8461 cttttgcccg ctttcttccc tggaaatacc tttccgccgc ttgccggccg gccggttatt
     8521 ccgcccattc tccggctgaa tggatcggag gaccgggatg gctatctcct gccgaaggcg
     8581 gcctttccgc aggatccggg ctcaaaataa atttgcattt cttaaaaccg ttcacttact
     8641 ttgcacttca aagtactttt aaatgagcac ttcacaaaac ctcgaaaccc taaaggacat
     8701 caaacggatg atggagcgca gcagccgttt catcagctta agcggctgga gcggaatcac
     8761 cgcggggatc tgcgccctgg ccggcgcatg gatcgccagc aaccgcatca gtacctatta
     8821 ccggaatgaa tattcaacca gtccggcctg tccttcctgc ctgaaaagtg atttactgac
     8881 gatcggatcg ctggtgttca ttgctgcttt tgcttcagcc atgttattta cctttttgaa
     8941 aagtaaaaaa gagggcgtgg ctatctgggg caatgcagcc agaagattac tctggaatac
     9001 cttactgcct atggccgccg ggggcttcct cctctggcgc atgatcgagc tgaaacaata
     9061 cgaattcata gcttccggat cgcttatttt ttacggactt gccctggtta atggaagcaa
     9121 gtataccatg ggagaggtcc ggtggctcgg ttatggccaa atcgtgctgg gaatcatcaa
     9181 tctctgggct gtacattccg
//
LOCUS       DASYCU010000566         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2876922, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000566 DASYCU010000000
VERSION     DASYCU010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876922"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..880
                     /locus_tag="VGM24_11410"
     CDS             176..880
                     /locus_tag="VGM24_11410"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY4156032.1"
                     /translation="MKNLFLLTMASLLLTAGYSQQAGDKNLQNRPVAPFHGISVSDGI
                     DLYISGGPQSVAVSAADPEYRDHIKTEVENGILKIYMEKGYHRSRDNAKLKAIVSLTN
                     LDRLGASGGSDTYLETALTVNELHVDLSGGSNLKGKITAGLLDIRQSGGSRVDLNGLV
                     KNLAVEASGGSDLRGYDLVSDYVAVQASGGCNAFVTVNRELKVVASGGSGVFYKGPAN
                     IKQIISSGSSRVVKKG"
     gene            919..2745
                     /gene="yidC"
                     /locus_tag="VGM24_11415"
     CDS             919..2745
                     /gene="yidC"
                     /locus_tag="VGM24_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY4156033.1"
                     /translation="MGMDRNTIIGFVLLGLLLFTYLFTATRSSQELQRQKQHQADSLA
                     VVQKLQESRMAAHDTAAGKAVIRDTVAGWGHAMEGTEKLITVENELLKIVFSNKGGQP
                     KEVILKHFKEFDSTLVRLVDSSLSNRVNYPINTVTNQTGQIADLYFNEGELQKSPDGT
                     QKLVFRLPAPNGESIIHEYVIHPNSYLVDWNLITTQANNLFSQNNLNLDWHVQMKQTQ
                     PYVYYEKRLTNVCFSVDHEFDYISSKTDKTFEKPVQWVSATQQFFNRTLISKSGFEGG
                     NVHWTREPEDSSALLGTADVHLQAKIPGPAATLNFQWYYGPNDYHILKTAAPEMDRIV
                     DLGRDMYSFVRPINIYVIMPVFNFFKRFVSSYGIAILLLTLFIRLLTSPLVYSSYLSG
                     AKMKALRPEIDAMKKRVGGDQQQIGVEQMKLFREAGVNPLGGCIPALLQIPIFFALYT
                     FFNSNVALRGQPFLWAKDLSIYDVVVKLGFNIPAYGNHISLFATIAVITSFLISLYGM
                     SMTPDQSNPAMKYMPYFMPVMLLLFFNRLPAALTWYYTVSNLITLTLQWVIQNYIIDH
                     DKILTKLQANRNKPKTKPKWQERFEQMQEAQKKAQQLKGKKP"
     gene            2751..3398
                     /locus_tag="VGM24_11420"
     CDS             2751..3398
                     /locus_tag="VGM24_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156034.1"
                     /translation="MKNKQLYIFFLLVPGFLSAQKRVGELTLVYNYVVASGKDASKQM
                     NASTTIYIKGNLSRSEANSNLFSSATIYDSKTGSGVILKEVNGQKLLIRLNEENWNQK
                     NSRFNNIHFTNTAETRIIAGYSCTRATASTPEGYQISAFYTRDLIPENKSYDAHFKNL
                     DGLPLEYEFSKGDLHIKYVLASINLNPVPASKFDIPTNGYREMTYEESLRLKLNN"
     gene            complement(3432..>3621)
                     /locus_tag="VGM24_11425"
     CDS             complement(3432..>3621)
                     /locus_tag="VGM24_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156035.1"
                     /translation="GTLMNFRPQADNRISLNAMITYQRGNTTFILPYQYKVTIGPVTN
                     NSGKTNLQFLGVKIQMPK"
BASE COUNT          986 a          846 c          891 g          898 t
ORIGIN      
        1 tatccttttt aggaaacagc accccgggaa cttggaaaca accgccggtt gtttcactgg
       61 caaatatacg tgacatttat atccgatcat ccgtcaaata aaaaacggcc ggtgaagcaa
      121 caaaccatgc gcccggctgt cttttgctta ttcttaactc aacgaacaga tcatcatgaa
      181 aaatcttttt cttctgacaa tggccagtct cctcctgacg gcgggttatt cgcagcaggc
      241 cggcgataaa aacctgcaaa accgtccggt ggccccgttt catggcattt ccgtatccga
      301 tggaattgac ctgtatattt ccgggggtcc gcagtcagtg gccgtcagtg cagccgatcc
      361 ggaataccgg gaccatatta aaacggaagt ggaaaatgga attttgaaaa tttatatgga
      421 aaagggatat cacagatccc gggataatgc caaattgaag gcaattgttt ctctgaccaa
      481 tcttgaccgg ttgggtgctt ccggcggatc agatacttac ctggaaacag cactgaccgt
      541 aaatgaactt catgttgatt tatccggcgg aagtaattta aaaggaaaaa taacggccgg
      601 tctgctggat atccgccaat cgggtggttc ccgcgtagac ctgaacggcc tcgtgaaaaa
      661 tctggccgta gaggccagtg gaggaagcga tcttcgtgga tatgatctgg tcagcgatta
      721 cgtggccgtt caggccagcg gaggatgtaa tgcttttgtt accgtaaacc gggagctgaa
      781 agtagtggcc agcggcggaa gcggagtatt ctacaagggc ccggcaaaca taaagcaaat
      841 tatatccagc ggatccagcc gtgtggtaaa gaaaggctaa gatcccccta tctttgcact
      901 ccttaaattg agtgtaaaat gggtatggat cgcaatacga taattggttt tgtgctgttg
      961 ggcctgttat tgtttaccta tcttttcacg gccacccgga gcagccagga gttgcaaaga
     1021 caaaaacagc atcaggcaga ttccctggcc gtggttcaaa aactgcagga atcaaggatg
     1081 gccgctcatg atacggcggc cggcaaggcg gtgatccggg ataccgttgc gggatggggt
     1141 catgcaatgg agggcacgga aaaactaatt accgtggaaa atgaactgct gaaaatcgtt
     1201 ttcagcaaca aaggcggtca gcccaaagaa gttattttaa aacatttcaa agaatttgac
     1261 agtacactgg tcaggctggt ggattcctcc ctctctaacc gggtgaacta cccgatcaat
     1321 acggtcacca atcaaaccgg ccagattgcc gatctttatt tcaatgaagg agaacttcag
     1381 aaaagcccgg acggcaccca gaaactggtc ttcagacttc ccgcgcccaa cggggaatcc
     1441 attatacatg aatatgtaat ccatcccaac agttacctgg tggactggaa tctgatcacc
     1501 acgcaggcca acaatctgtt ctcccagaat aacctgaacc tcgactggca tgtgcaaatg
     1561 aagcagacac agccttatgt ttattatgaa aaaaggctga ccaacgtttg tttttcggtt
     1621 gatcatgagt ttgactatat ttcttccaaa acagacaaga cctttgaaaa accggttcag
     1681 tgggtatctg ccacccagca gtttttcaac aggaccctca tttcaaaaag cggatttgaa
     1741 ggcggaaatg ttcattggac cagggaaccg gaggacagtt ccgcgctttt gggaacagcc
     1801 gatgttcacc tgcaggcaaa gattccgggt ccggcagcca cccttaattt tcagtggtat
     1861 tatggcccga acgactatca tattctcaaa accgcggctc cggaaatgga ccggatcgtg
     1921 gatctgggca gggacatgta ttcctttgta cggccgatca atatttacgt gatcatgccg
     1981 gtcttcaatt ttttcaaaag gttcgtaagc agttatggta tcgctattct gttgctgacc
     2041 ctgtttatcc ggttgctgac gtctcccctg gtgtacagca gttacctcag cggagcaaaa
     2101 atgaaagcgc tgcgtccgga gatcgatgcg atgaagaaaa gggtcggcgg agatcagcaa
     2161 cagatagggg tggagcagat gaagctcttc cgggaagcgg gggttaatcc cctgggcggt
     2221 tgtattcccg ccctgctgca gattcccata ttctttgccc tgtatacttt ctttaactcg
     2281 aatgttgccc tgcggggcca gcccttttta tgggcgaagg atctatccat atatgatgtt
     2341 gtcgtaaaac tgggattcaa tatcccggca tatggaaacc atatcagttt atttgccacc
     2401 atcgccgtga tcaccagctt tctgatctcg ctttacggca tgagcatgac accggaccag
     2461 agcaatccgg cgatgaaata tatgccctac tttatgccgg tcatgctgct tttgtttttc
     2521 aaccggcttc cggcggcgct tacctggtac tatacggtaa gcaatctcat caccctgacc
     2581 ctgcagtggg tgatccagaa ctatattatt gatcacgata agatcctgac aaagctccag
     2641 gcgaaccgga acaagccgaa aaccaaaccc aaatggcagg agcggtttga gcagatgcag
     2701 gaggcgcaaa aaaaggcaca acagcttaag ggcaaaaaac cctgatcgga atgaagaata
     2761 agcaactgta tatcttcttt ttgctggttc ccgggtttct ttctgcccag aaacgggttg
     2821 gagaacttac cctggtatac aattatgttg tcgcgtccgg caaggatgct tccaaacaga
     2881 tgaatgcttc tactacgatc tatatcaagg gaaatctcag ccgttccgaa gcgaatagca
     2941 acctgttttc ttcggccacc atttatgatt caaaaaccgg atcgggtgtg atcctgaaag
     3001 aggtaaacgg tcagaaattg ctgatccgct taaacgaaga aaactggaat cagaagaata
     3061 gccggttcaa taatatccat tttaccaata ccgcagagac ccgcatcatt gcgggatatt
     3121 cctgtacccg ggccacggcc tcgacaccgg agggttacca gatctcggct ttttataccc
     3181 gcgatctgat tccggaaaac aaatcctatg atgctcactt taaaaatctg gatgggttac
     3241 cgctggaata tgaattcagc aaaggggatt tgcatattaa atatgtattg gcctccatta
     3301 acctgaatcc ggtgccggcc tcgaaattcg atattccaac caatggctac cgcgagatga
     3361 cctatgagga aagcctcagg ctaaaattga ataattaagg ataaaacgga aggggaagtt
     3421 gagcaaccgg cttatttcgg catctggatt tttaccccca gaaactgaag attggttttc
     3481 ccgctattgt tggtaacggg cccgatggtc actttgtatt ggtaggggag aataaacgtt
     3541 gtatttccgc gctgataggt gatcatggca ttcagggaaa tgcgattgtc cgcctggggc
     3601 cggaagttca tcagggttcc g
//
LOCUS       DASYCU010000567         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2586868, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000567 DASYCU010000000
VERSION     DASYCU010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2586868"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VGM24_11430"
     CDS             <1..225
                     /locus_tag="VGM24_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156036.1"
                     /translation="IYRNSKIRLVRDGIVIYPIGEGATAELASLKRFKDDAKEVPSGM
                     ECGLTIKNYSDIRVGDIVEAYEEEEVKRTL"
     gene            360..1721
                     /locus_tag="VGM24_11435"
     CDS             360..1721
                     /locus_tag="VGM24_11435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY4156037.1"
                     /translation="MKNIIFTGIFMLPGLAVFSQQRVVADKIIGIVGDKIVLKSEIQI
                     ANEDIRRQGGQEQGDCALLDGMLTQKALVLQAQKDSIPVTDDEVEAEIDQRIRYFISQ
                     YGSKEALEQISGRTIYQMREDFREPIKEQRMAAGMRSKIVDGIKITPTEVQEFFDKIP
                     KDSLLFFDSKLQLGQIVVYPKPSRDIELLAIEELNEYKKEVESGAKRFETLETLYSDD
                     PGTKNNGGTYQLNRNDKQWDPVFLANAFRLKEGQISPVIKSKFGYHIIQMVSRSGDDA
                     IVRHILRIPQITEKESGEAISKLDSIRNQLVDGSLSFGEAVAKYSDDENAKFTAGMLL
                     ARDGSNYLQIDELDKNLVLLLKSTNLKPGEYSKPTVFTDDRGKKGVRVIYLLSKSEPH
                     RENMRDDYNWIAQRALDEKKNAVLQKWFQSRIATYYIMIDKDYQHCPNLGKWETKQFA
                     SAN"
     gene            1847..2488
                     /locus_tag="VGM24_11440"
     CDS             1847..2488
                     /locus_tag="VGM24_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4156038.1"
                     /translation="MIRILIADDHKLIRETWTHILNNDVDARFKVIATCENGEDAVKL
                     TSQKHPDVVLMDINMVPFSGIEATRRIREIWPQSKIIGVTMHSQPSYARKMMQIGASG
                     YVTKNSSREEMMEAIVEVHQGNKYICSEIKDIISDQSEKKPVVQADINALTEREMEII
                     NLIKKGNSSKEISNFLQISLKTVEVHRHNILKKLNLKNSASLVNFMNTRTTYI"
BASE COUNT          770 a          580 c          610 g          576 t
ORIGIN      
        1 atctaccgga attccaagat caggctggtg cgcgacggca tcgtgatcta tcccatcggg
       61 gaaggagcca ccgcagaact cgcttcactg aaaagattta aggatgacgc caaggaagtg
      121 ccttccggca tggagtgtgg actcaccatc aaaaactaca gtgatatccg ggttggagac
      181 atcgttgaag catacgaaga agaagaagta aaaagaacgc tttagcccgg gattctcttc
      241 cggatagcgg gaaagtataa cgggaacaga taaccggcat aagcaatatt ttgttaaatc
      301 gcccggacct tctttcaaca ggggggaacc ggcgtatttt tgaacgtggg catttcttta
      361 tgaagaatat aatttttacc ggaattttca tgctgcctgg attggctgtt tttagtcagc
      421 agagagtggt tgcggataag atcatcggca ttgtagggga taagatcgtg ttgaaatcgg
      481 agatccagat tgccaatgag gatatccgca ggcagggcgg ccaggaacag ggcgattgtg
      541 ctttgctgga cggaatgctt acccaaaaag cactggtatt gcaggcgcaa aaagattcta
      601 tcccggtaac cgacgacgaa gtggaggcgg aaattgacca gcggatacgg tatttcattt
      661 cgcagtacgg cagcaaggag gccctggaac aaatttccgg aagaacgatc tatcagatgc
      721 gggaggattt ccgggaacct atcaaagaac aaagaatggc tgcgggcatg cgcagcaaga
      781 tcgtggacgg tatcaagatc acccctactg aagtgcagga atttttcgat aagatcccaa
      841 aagacagtct gctgttcttt gattcaaaat tacagctcgg acagatcgtg gtctatccga
      901 aacccagccg ggatatcgaa ctgctggcca tcgaagaact gaatgagtac aagaaagaag
      961 tggaaagcgg agccaaacgc tttgagaccc tggaaacttt atactccgat gatccgggaa
     1021 caaaaaacaa cggcggtacg taccagctga accggaacga caagcagtgg gatccggtat
     1081 tcctggccaa tgcgttccgg ttgaaagaag ggcagatctc tcccgttata aaatcaaaat
     1141 tcggttacca tatcatccag atggtcagcc gttccggcga cgatgccatc gtgcgccata
     1201 tcctccgcat tccgcagatc accgaaaaag aatccggcga agccatcagc aaactggatt
     1261 ccatccggaa tcagttagtg gacggttcac tcagcttcgg ggaagccgtt gccaaataca
     1321 gcgatgatga aaatgcgaaa ttcacggccg gtatgcttct tgcccgggat ggttcaaatt
     1381 atttacagat cgatgaactg gataaaaacc tggtcctcct gttgaaaagc accaacctga
     1441 aacccggcga gtattccaag ccaaccgtct ttacggacga ccggggaaag aaaggcgttc
     1501 gcgttattta tcttttatcc aagtctgaac cccatcggga aaatatgcgc gacgactata
     1561 actggatcgc ccagcgggcc ctggatgaga aaaagaatgc cgtacttcag aaatggtttc
     1621 aatccagaat tgccacctat tatatcatga tcgataagga ttaccagcat tgccccaacc
     1681 tgggaaaatg ggagacaaaa cagtttgcat cggccaacta actaagtgta aacacttagc
     1741 acccataaaa aacagctcct cagcgtttca ttttcagtgt agttatagta tattacaaat
     1801 cataataagc cgagttgtaa tcccttcacc tattaaagcc acagatatga tcagaatatt
     1861 aattgcggat gaccacaagc tgataaggga aacctggact catattctga ataacgatgt
     1921 tgacgcccgg tttaaagtga ttgcgacatg tgagaatggc gaagatgccg ttaagctcac
     1981 cagccagaag catcccgatg tcgttctcat ggatatcaac atggtaccct tttccggaat
     2041 tgaagcaact cgccggatcc gcgaaatatg gcctcagtcc aaaatcatcg gggtcaccat
     2101 gcattcacag ccttcctacg ccaggaaaat gatgcagatc ggcgccagcg gatacgtgac
     2161 taaaaattct tccagggaag agatgatgga agccattgtg gaagtgcacc agggaaataa
     2221 atatatctgc agcgagatca aggatattat ttcggaccag tccgaaaaga aaccggtggt
     2281 gcaggctgat attaacgcat tgacagagag agagatggag atcatcaacc tgatcaaaaa
     2341 gggcaattct tccaaagaaa tatccaattt cctgcagatc tccctcaaaa cagtggaagt
     2401 tcaccgccat aacatactta agaagctgaa cctcaaaaac tcagcctccc tggtcaattt
     2461 tatgaatacc cggaccacct atatctgaaa tccggtttcc catctttctt ttaattaagt
     2521 taactttgcg cttcct
//
LOCUS       DASYCU010000568         5795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4180474, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000568 DASYCU010000000
VERSION     DASYCU010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4180474"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="VGM24_11445"
     CDS             complement(<1..1708)
                     /locus_tag="VGM24_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HEY4156039.1"
                     /translation="MKRIIPLVILAFLLIPAAEAQQKHSFVFSRDQFMLDGKPFRIIS
                     GEIHPARVPAEYWRHRIEMAKAMGCNTIAAYIFWNYHETEPGKFDFQTGNRNISRFLQ
                     ICKEEKMWVIMRPGPYVCAEWDLGGIPSYLLKIPDIKFRCMDSRYIKAVGGYFRRLSQ
                     VLKPFMVVNGGNILMLQIENEYGSYGNDKQYLKTLKRLWIQNGINGPFYTSDGPTAYM
                     LDAGTLDSAAIGLDSGGSLGDFETAAKQNANVPVFSSETYPGWLTHWGEAWQRPDTND
                     LKKQVEFLLKNNKSFNFYVIHGGTNFGFTAGANAFSPTQYQPDITSYDYDAPITEQGR
                     ATPKYYLLRNLIRQYVSYSVPDVPASIKTIRVPPVKMKRTMNLWSLVKPSITSPQPEP
                     MEAYGQNQGLIVYRTRLIGHKSGTLKIWEPHDYAMVFLNGKFIDTVYRDGGKWEVKLP
                     QTNEKDPLLEIVVEGMGHINFGQFIIDRKGITDRVTLNGMTLMNWSIALLPMDEHFIR
                     TARPDLSVHPEPGKPCNFFEGKFDLKETGDTFLDVSRFSKGILYVNGHNLGRYWNKGP
                     QER"
     gene            complement(1800..2279)
                     /locus_tag="VGM24_11450"
     CDS             complement(1800..2279)
                     /locus_tag="VGM24_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY4156040.1"
                     /translation="MKITSQEEFGLRILIRIAGCRTEAGMSIPQLSKAEGLTSHYVAK
                     LTRTLRMAGFINSTPGYKGGYVLAKPAKQIRIREVLKVLGGVLFDQDFCGMHSGELNL
                     CTNSVDCSARSLWKMIQFTVDQLLDKITLHDMANTEKESTKFLTRLLEQKNEIALKA"
     gene            complement(2305..2616)
                     /locus_tag="VGM24_11455"
     CDS             complement(2305..2616)
                     /locus_tag="VGM24_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEY4156041.1"
                     /translation="MNTEELKEKIIECLKSIYDPEIPVNIYEMGLIYEVNVLPINNVQ
                     IVMTLTAPGCPAAQSLPVEVDQKLREIEGVNDVHVSVTWTPAWDRSMMSETAQLELGM
                     L"
     gene            complement(2613..3065)
                     /locus_tag="VGM24_11460"
     CDS             complement(2613..3065)
                     /locus_tag="VGM24_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY4156042.1"
                     /translation="MSTRNTIAETEHEIVEEFSLFDSWDDKYEYIIDLGKKLPPLEER
                     FKLDENRVKGCQSTVWLASDYRDGRVFYKADSDALIVKGLISMLIRVLSGHTPDEILG
                     DKLAFIQEIGMTTHLAQTRSNGLLSMVKQMKHFALAYKIKNDSLPERI"
     gene            complement(3068..4312)
                     /locus_tag="VGM24_11465"
     CDS             complement(3068..4312)
                     /locus_tag="VGM24_11465"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY4156043.1"
                     /translation="MMPSPTIRKNLDIAAIREQFPVLNREVKGRPLVYFDNAATSQKP
                     QAVIDALVNYYSGYNANIHRGIHTLAEEATAAFEETRDAVQQFIHAPSREEIIFTRGT
                     TESINLVAYTWGRQNLRAGDEIIISIMEHHSNIVPWQILCEEKGAVLKVIPLSDQGEL
                     LMDEYKKLLGPKTKLVAVVHASNVLGTINPVREIIELAHAAGAVVLVDGAQSAVHLDI
                     DVQEMDCDFFAFSAHKLFGPTGVGVLYGKKKILESMPVFLGGGEMIREVTFAKTTYND
                     LPYKFEAGTPNIADVIVFRTALAFIQETGRELIRKHEQDLLVYATEQLKMIPGLRIIG
                     EAKEKVSLISFVIDQLHPQDLGILLDNRGIAVRTGHHCAEPLMQRYHIPGTVRASFSV
                     YNTRTEIDELVTGLKKAIKILS"
     gene            complement(4309..5619)
                     /gene="sufD"
                     /locus_tag="VGM24_11470"
     CDS             complement(4309..5619)
                     /gene="sufD"
                     /locus_tag="VGM24_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY4156044.1"
                     /translation="MNTIEYFKERFDQWQPGNGSNGLGAIRQEAFNAFSRMGIPGSKN
                     EEWKYTRVAGLFNQEYQFPPDSLNTSVTAKDLDGLRLPGHEQAHELVFVNGFFSFPLS
                     IMRSSDLTILPLEDAAKNDFRELVAKEFGDSSKYIKDGIHALNTAFVHGGVFIHIRKG
                     LVLEHPVYIYNITDARSSNILAQPRSLIHINENANVQLVETYMTLGDNQSFTNQVMEV
                     TVEKDARLEYYKIQNDSDHTNQVSTTHIRQIGKSYVHTVTISLNGGLVRNNLNLVLDA
                     KYCEAHLYGLYFQTGQSHIDNHTVVDNIKPNCLSNELYKGIMNDQSTGVFNGKIFVQP
                     QAQKTNAYQSNKNVLLSNSTSVNTKPQLEIFADDVKCTHGCTVGKLNEDGLFYLQSRG
                     ISEKTARTLLLRAYASDILEHIKPAPIRAYTEQLIVERLESDIS"
BASE COUNT         1385 a         1474 c         1317 g         1619 t
ORIGIN      
        1 gtctttcctg cggtccttta ttccaatatc ttccgagatt atgaccattt acatacaata
       61 tgcctttcga aaaccggctc acgtcaagaa aagtatcccc ggtttcctta agatcgaatt
      121 taccctcgaa gaaattgcag ggcttgccgg gttccggatg aaccgacaaa tccggcctgg
      181 ccgtcctgat aaaatgttca tccatcggca gcaaagcaat actccaattc attaaggtca
      241 tcccgttaag cgtgacccgg tcagtgatcc ccttccggtc aatgataaat tgcccgaaat
      301 taatatggcc catgccttcc acgacgattt ccagcaaagg atctttttca tttgtttgcg
      361 gcagctttac ttcccatttt ccaccatccc ggtaaacggt atcaataaat ttcccattca
      421 aaaacaccat ggcataatca tgcggttccc agattttcaa cgtgccgctc ttgtggccaa
      481 tcagccgggt ccggtaaacg ataagcccct ggttttgtcc ataggcctcc atgggttcag
      541 gttgcggact ggtaatggaa ggcttcacca gactccacag attcatggtc cgtttcatct
      601 taaccggagg cacccggatt gtttttatgg atgccggcac atccggcacg gaataggaaa
      661 catattgcct gatcagatta cgcaggaggt agtatttggg tgtagcccgg ccctgttccg
      721 tgatgggcgc atcataatca taactggtta tgtccggttg gtattgcgta ggcgaaaagg
      781 cattggcacc ggccgtaaat ccgaagttgg taccaccgtg aatgacatag aaattaaagg
      841 acttattatt ttttaacagg aactcaacct gctttttcag atcattggta tccggccgct
      901 gccaggcttc accccaatgt gttaaccagc cggggtaggt ttcactgctg aaaaccggga
      961 cattggcatt ctgtttcgct gccgtttcaa aatcgccgag tgaacccccg ctgtccaaac
     1021 cgattgctgc actatccagc gtacctgcat ccagcatata ggcggttggg ccatccgaag
     1081 tataaaaagg cccgttgatt ccgttttgaa tccataatct cttcagtgtt ttcaggtatt
     1141 gcttatcgtt cccgtaactt ccgtattcat tttctatctg cagcattaaa atattgcccc
     1201 cgttgaccac cataaatggt ttcaacacct gggaaagccg gcgaaaataa cctcccactg
     1261 cctttatgta acgggaatcc atgcaccgga atttaatatc cggaatctta agcaggtagg
     1321 agggaatgcc acccaggtcc cattccgcac agacataagg gcccggacgc atgataaccc
     1381 acattttttc ttccttacat atctgcaaaa agcggcttat attcctgttg cccgtttgaa
     1441 aatcaaattt gcccggctct gtttcatggt aattccagaa gatatacgct gcgatggtat
     1501 tgcatcccat ggcttttgcc atttcaatcc ggtgccgcca gtactctgcc ggcacgcggg
     1561 caggatgaat ttcgccgctg atgatgcgga agggtttgcc atccagcata aactgatccc
     1621 tgctaaatac aaacgaatgc ttttgctgag cctccgctgc cggtatgagc aaaaaagcaa
     1681 ggatcaccag gggaattatt cttttcataa actcttttac gaaacggttt ctgatagtgg
     1741 agcgacccga tgatgatccg cgggagtccg gtggggtagg atccgctatt cgttctgcac
     1801 tacgccttca gcgcaatctc gttcttctgt tccagcaaac gggtcaggaa tttagtggat
     1861 tccttttcgg tattcgccat atcgtggaga gtaatcttat ccagtaactg atcaaccgta
     1921 aactggatca ttttccagag agaacgggca gaacagtcca cggaattggt acagaggttt
     1981 aattcaccgg aatgcatacc gcaaaaatcc tgatcaaaca aaacgccgcc caatactttc
     2041 aatacttccc ggatccggat ctgttttgcg ggtttggcga gtacatagcc gcccttataa
     2101 cccggcgtgc tgtttataaa cccggccatg cgaagcgtgc gggtcagctt ggcaacataa
     2161 tgactggtca ggccctctgc cttgctcaac tgcggaatgc tcatgccggc ttcggtccgg
     2221 caacccgcaa tgcggatcag tatgcgtaag ccgaattctt cctgcgaggt tattttcatt
     2281 tgaattttta aagtttagcc ttctttataa catacccaat tccagttgtg ctgtttcgga
     2341 catcatgctc cggtcccagg ccggggtcca ggttaccgac acgtgcacat cgttcacgcc
     2401 ttcaatttcc cggagtttct gatccacttc aaccggtaag gattgcgctg cggggcaacc
     2461 cggcgccgtt aaagtcatta cgatctgcac attattgatg ggaagcacat tgacttcata
     2521 gatcagcccc atttcgtaaa tgttcaccgg tatttccgga tcataaatgc ttttcaggca
     2581 ttcaattatt ttttctttca gctcttcggt attcatattc tttcgggtaa tgaatcgttt
     2641 ttgatcttat aggccagggc gaaatgcttc atttgtttta ccatagacaa taatccgtta
     2701 gaacgcgttt gtgccaggtg ggtcgtcatg ccgatttcct gaataaaagc cagtttatcg
     2761 cccaggatct catccggcgt atgtccggat aaaacccgga tcagcatgct gatcaatcct
     2821 tttacaataa gggcatcgct gtcggcctta taaaagaccc ttccatcgcg atagtcggat
     2881 gccagccaaa ccgtggactg acagccctta acccggttct cgtccagctt aaaccgctcc
     2941 tccagcggag gaagtttttt tcccaggtcg ataatatatt cgtatttatc atcccaggaa
     3001 tcgaacaggg aaaactcctc tacgatttca tgctccgttt cagctatcgt attccgggta
     3061 ctcattttca ggataagatc ttaattgctt tcttcagtcc cgtcaccaat tcgtctattt
     3121 ccgtccgggt attatatact gaaaaagagg cgcggaccgt gcccggaata tgataccgtt
     3181 gcatcagggg ttccgcgcaa tgatgaccgg ttcttaccgc aatgccccgg ttatccagca
     3241 ggatgcccag gtcctgcgga tggagctgat caattacaaa agagatcagg ctcacctttt
     3301 cttttgcttc gccaatgatc cggagtcccg ggatcatttt taactgctct gttgcataaa
     3361 ccagcaggtc ctgctcgtgt ttccggatca gctccctccc tgtttcctgg atgaaggcca
     3421 gcgcggtcct gaatacgatc acatcggcaa tattgggggt gcccgcctca aacttatacg
     3481 gcaggtcatt ataggttgtt tttgcaaacg tgacttcccg gatcatttcg ccgcctccca
     3541 gaaaaacggg catggattca agaatttttt tcttcccgta caatacaccc acgccggtag
     3601 gaccgaataa tttatgggcc gaaaaggcaa aaaagtcgca atccatttcc tgtacatcaa
     3661 tgtccaggtg aacggcagat tgcgcgccgt ccactaaaac cactgcaccg gctgcatggg
     3721 ccagttcgat gatttccctg acgggattaa tagtgcccag cacattggaa gcatgaacta
     3781 ccgccaccag ttttgttttc gggcccagca gttttttata ttcatccatt aacagttctc
     3841 cctggtcgct gaggggaatt accttcagga cggccccttt ttcttcacac aggatctgcc
     3901 agggaacaat attggagtgg tgttccatga tagaaatgat tatctcatcg ccggcccgga
     3961 ggttttgtct tccccaggta taggccacga ggttaatgct ttccgtggtg ccgcgggtaa
     4021 atatgatctc ttcccgggaa ggagcgtgaa taaactgctg aaccgcatcc cgggtttctt
     4081 caaacgccgc tgttgcttcc tccgccaggg tatgaatgcc ccggtggata ttggcgttat
     4141 agcctgaata ataattgacc agcgcatcaa tcacggcctg cggtttctgg gaagtggccg
     4201 cgttgtcaaa ataaaccagg ggccgtcctt tcacttcccg gttcaatacc ggaaactgtt
     4261 cgcggatggc ggctatgtcc aggttctttc ttatggtggg agaaggcatc atgatatatc
     4321 agattctaat cgttctacaa tcaattgctc agtataggcg cggatcgggg caggtttgat
     4381 atgctccagg atatcggatg cataagcccg taataataag gtgcgggccg ttttttccga
     4441 aatacccctg gattgcagat aaaataagcc atcctcattc agcttcccga cggtacagcc
     4501 atgggtgcat tttacatcgt cggcaaatat ctccagctgg ggctttgtgt ttacgctggt
     4561 gctgttggaa agcaatacat tcttattcga ctgataggca tttgtttttt gtgcctgggg
     4621 ctgtacgaat atttttccgt tgaatacacc ggtggactgg tcgttcatga ttcctttgta
     4681 cagttcattg cttaagcaat tcggttttat attgtcaacc accgtatggt tatcaatatg
     4741 gctttgaccc gtctggaaat atagcccgta caaatgcgct tcacaatatt tagcgtccag
     4801 caccaggttg agattatttc ttaccaggcc accgtttaac gaaatggtga ccgtatgcac
     4861 atagctcttt ccaatctgcc ggatatgggt ggtacttacc tggttggtat gatcgctgtc
     4921 attctgaatt ttataatatt ccaggcgggc atccttttcc acggtgacct ccatgacctg
     4981 gttggtgaaa ctttgattgt ctcccagggt catataggtc tccaccagct gcacgtttgc
     5041 attttcatta atgtgaatga ggctgcgcgg ctgggcaagg atattggaag aacgggcgtc
     5101 cgtaatatta taaatgtaaa ccggatgctc cagcaccagc ccttttctga tatggatgaa
     5161 aactcctccg tgcacaaagg cagtattaag ggcatgaata ccatctttaa tgtatttgct
     5221 gctgtcccca aattcttttg caaccagctc cctgaaatca ttttttgccg catcttccag
     5281 gggaagaatg gtcaggtcgg atgatcgcat aatggaaagc ggaaaagaaa aaaaaccgtt
     5341 tacaaatacc agttcatgtg cctgctcgtg cccgggcaat cgcaacccgt ccaggtcctt
     5401 tgccgtcacg gaagtattca gggagtccgg cggaaactga tattcctggt tgaacaaccc
     5461 ggcaacccgg gtatatttcc attcttcgtt tttggagccc ggtatgccca tccggctaaa
     5521 cgcgttgaat gcttcctgcc ggatggcgcc cagtccatta cttccgttac ccggctgcca
     5581 ctggtcaaaa cgctctttaa aatattctat cgtgttcatg cattgttttt attgatcccc
     5641 gaagggggtg ctaaacggct tccaccgcac cggcttcatt ccggataaaa tcatatcctc
     5701 tttcttccag ttcaaaggcc agttccttgg taccggattt tacgatccgg ccattgtaca
     5761 gaacgtgcac ataatccgga acaatataat ccagc
//
LOCUS       DASYCU010000569         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3021649, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000569 DASYCU010000000
VERSION     DASYCU010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3021649"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGM24_11475"
     CDS             <1..259
                     /locus_tag="VGM24_11475"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156045.1"
                     /translation="RVRFLITVPYKSDFRKARDLISEEIRKNDKILKDPAAAVTVDSF
                     ANNSVNFEVSFWVPDLSETSSLRNGLMLDIFESFSKNGIVM"
     gene            complement(291..1721)
                     /locus_tag="VGM24_11480"
     CDS             complement(291..1721)
                     /locus_tag="VGM24_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4156046.1"
                     /translation="MKIKKNNSRRDFLKSTSLAAAGYLIVPRHVLGRGFVAPSDKLTI
                     AGIGAGGKGQSDLFEFSKSPNANIAYLCDVDDRQIKASVERFPKAKRYYDFREMLDKE
                     HKHIDAVSVSIPDNCHAVAAIAAMQLGKHVYVQKPMTHDIYEARMLTEAARRYKVVTQ
                     MGNQGASGDGVRTMQEWYNAGIIGDATAIHVWTNRPVWPQGFGTPKSTDPVPPELKWD
                     IWLGPAQEQPYHKEYVPFNWRGFWAFGTGALGDMGCHLIDPAFKTVGLGYPSEVECSA
                     VNIVEKMWTPDYFPESCPAASSIILKFPGKAGKPDVKLYWTDGGILPERPDELGPDEL
                     FGDRDGCGVLITGTKGKMMCGTYGSNPTLLPTSRNKEVNVPQTLARVPEGHYVQGVNA
                     CIAGFGQKELSSSFDYAGPLTETVIMGNLALRSWYVKAPSTNGNGRRGNEFPGRKKLL
                     WDAANMKITNFDEANQFVRRTYRAGW"
     gene            complement(1785..>2546)
                     /locus_tag="VGM24_11485"
     CDS             complement(1785..>2546)
                     /locus_tag="VGM24_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4156047.1"
                     /translation="GTAPFRDYNPAYLPEIWRGWVDFGSGSLGDMGCHFIDVPYRALK
                     LGYPVAVETSIGSVYTGYFQELINPDGFPPSSKTHIQFPQRGSLVPLEMVWYDGGILP
                     RRPDELLPDEPMGEAGAGGILFDGTKGKLMAGLFGRNPTLLPTRLMKETTLPESKYPF
                     VDGGSEGHQQQWVKACKKGYGTYTSSDFSVAGPLTETVIMGNLAVRSYQYSEADAQGK
                     KIFPGRKKLLWNGEKMEISNFAPANRFVKREYRSF"
BASE COUNT          586 a          688 c          608 g          664 t
ORIGIN      
        1 aagggtccgc ttcctgatca cggttcccta taaatccgat ttccggaaag ccagggacct
       61 gatttcggaa gagatccgga agaatgataa aatattaaaa gatccggcag cagccgtaac
      121 cgttgattcc tttgcaaaca actccgtcaa ttttgaagtc agcttctggg tgccggacct
      181 gtctgaaacc agttccctcc ggaacgggct gatgctggat atttttgaat ccttttcgaa
      241 gaacgggatc gtgatgtaag gaaatcgcct ttaatcaact ttccattctg ctaccagcct
      301 gcccggtacg ttctccggac aaactgattg gcttcgtcaa aattggtgat cttcatattc
      361 gccgcatccc agagcagttt ttttcttccg ggaaattcat ttcctcttct gccatttccg
      421 ttagtggacg gagccttcac gtaccagctt ctcagcgcca ggtttcccat gatgacggtt
      481 tctgtaagcg gtccggcata atcaaaggaa gaactcagtt ccttctggcc aaagcctgcg
      541 atacaggcat tcaccccttg cacatagtgt ccttccggaa cgcgcgccag ggtctgggga
      601 acattcactt ctttatttct ggaagtgggc agtaaggttg ggttactgcc ataggtgccg
      661 cacatcattt ttcctttggt accggtgatc aaaaccccgc atccgtcccg gtcaccaaac
      721 aattcatccg gccccagttc atccggtctt tccggaagga taccgccgtc tgtccagtac
      781 agtttcacat ccggttttcc ggcttttccc gggaacttca atatgatgga agaggcggcc
      841 gggcagcttt ccgggaaata gtcaggcgtc cacatttttt caacgatatt gaccgcactg
      901 cactccactt cagaaggata tcccagtccg acggttttga aagccgggtc aatcaggtga
      961 caacccatat ctcccagagc gccggtgccg aaagcccaga aaccgcgcca gttgaaggga
     1021 acatattcct tgtgatacgg ctgctcctgt gccggtccga gccagatatc ccattttaat
     1081 tccggaggaa cgggatcggt gcttttcggc gttccgaatc cctgcggcca taccgggcgg
     1141 tttgtccata catgaatggc cgtagcgtcg ccgataatgc cggcgttata ccattcctgc
     1201 atcgtgcgca caccatctcc ggaggcgccc tggttgccca tttgggtaac cactttatag
     1261 cgtctggccg cctccgttag catacgggct tcataaatat cgtgcgtcat cggcttctga
     1321 acatacacgt gtttgcccag ttgcatggcg gcaatggcag ccacggcatg acaattatcc
     1381 ggtatggaaa cggaaaccgc gtcaatatgt ttgtgttctt tgtccagcat ttccctgaaa
     1441 tcataatacc gctttgcttt tggaaaccgc tccacggagg ccttgatctg gcggtcatcc
     1501 acatcgcaca aataggcaat attggcattc gggcttttgg aaaactcaaa caaatcgctc
     1561 tgtccctttc cgcctgcacc gatgccggct atagtgagtt tatcgctggg ggcaacgaaa
     1621 ccacgtccca gcacatgccg gggtacaatg aggtaaccgg ctgctgccaa agaagtagat
     1681 ttaagaaaat ctcttctgga attgtttttt ttgatcttca tcgaacttaa ccttttggaa
     1741 tgaaaaaata agaacctgtc tcattggtgt ggttagctat attcttaaaa ggaacggtat
     1801 tcccttttta caaaacggtt agccggggca aaattgctga tctccatttt ttcgccgttc
     1861 cagagcagtt ttttcctgcc ggggaatatc ttttttccct gtgcatcggc ttcactgtac
     1921 tgatagctgc gcaccgccag atttcccata atcaccgttt ccgtaagagg accggcaact
     1981 gaaaaatccg aactggtata cgtaccgtat ccttttttgc atgctttcac ccattgctgc
     2041 tgatggcctt ccgatcctcc atccacaaaa ggatatttcg attctggcag tgtggtttcc
     2101 ttcatgaggc gggtcggcaa tagcgttgga tttctgccga ataagccggc catgagcttg
     2161 ccttttgttc cgtcgaacag gatgccgccg gcaccggctt ctcccatcgg ctcatccgga
     2221 agaagttcat ccgggcggcg gggcagaatg ccaccatcgt accagaccat ttccagcggg
     2281 accagggaac cgcgctgcgg aaactggata tgtgttttgg aagagggcgg aaatccgtcg
     2341 ggattaatca attcctggaa atacccggta tagacggaac cgatactggt ttccacagcc
     2401 acgggatatc ccagtttaag cgcccggtaa ggcacatcta taaaatgaca acccatatct
     2461 ccgagtgatc ccgagccaaa gtcgacccac cccctccaga tttccggaag ataagcggga
     2521 ttataatccc tgaacggtgc ggttcc
//
LOCUS       DASYCU010000570         5276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3747396, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000570 DASYCU010000000
VERSION     DASYCU010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3747396"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VGM24_11490"
     CDS             <1..562
                     /locus_tag="VGM24_11490"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4156048.1"
                     /translation="EPNKEMRKKSVELLNHYPKYTTVDGKAENTNLPAGSVDAVVAGQ
                     AFHWFDAEKAKAEFKRILKPAGLVVLIWNERRTVTSFENEYDDLIRKHGKDYVKVDHR
                     NIRDTDIEIFFYPAICELNVFDNQQTLDLDGLKGRLLSSSYMPARGESGYPDMIRDLE
                     KLFEKHKENNRITINYDTKLYAGRFQ"
     gene            636..1109
                     /locus_tag="VGM24_11495"
     CDS             636..1109
                     /locus_tag="VGM24_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156049.1"
                     /translation="MTDSPDIRSLPGAYQLFLPEQVAAMLHLTQGDGKALVIIHENKM
                     QLEWLAQKIGEQYHSLYNAFIAGKARLKRKKRRVLIILLFVALAAIFNGPLAGIMQVD
                     RLYIKAFIFTLGIICCMQLLKYLAMGMAGSARGVDTELSVLELKELILKDFSPEE"
     gene            complement(1132..1665)
                     /locus_tag="VGM24_11500"
     CDS             complement(1132..1665)
                     /locus_tag="VGM24_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY4156050.1"
                     /translation="MKSILLISLFSLIGLIPPEHWNADEAKAKINFTVKGPFGTVHGS
                     FSGLKATIQFNEKEPGESALSASIEAKTVSTGISLRNSDLRNKEMWLNTDKYPTISFK
                     SRKIEKTSAGYKVSGDLTLKGTTRPVEIPFTFTSSGSTGIFKGQFTINREDYKVGKKG
                     GSVGDMITISLEVPVKK"
     gene            complement(1686..3104)
                     /locus_tag="VGM24_11505"
     CDS             complement(1686..3104)
                     /locus_tag="VGM24_11505"
                     /inference="COORDINATES: protein motif:HMM:NF040164.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HEY4156051.1"
                     /translation="MRKIVSVMLLVHNFLFIQAQQNIGIRSSNYAGIQAALLNPGAIA
                     DSKLKWDVNVLSLGTVFDNNFLYIPKGTIPVLGFRSIINGIKHEDKFYTRFDPGNPDK
                     RYNFTLSGEMLGPSFHAKIGRSQVIGFTLVSRSYANINDFTGHLAQSAFDYLRNKDLW
                     NKTFTDDKTRINGMNWLEYGFHYARVIWKNGNREVTGGISLKYLQGVAAAYVKNTSLT
                     YRVSDTTQLLFAHSNLDYGRTDYDSYRKIKNYQDLNHGHGLGGDIGIIYERLRETAAA
                     APVSKKWYEPEESKYVYRLGLSFIDVGAINFNRVSAAYHLQADSANFANWHNADFTNN
                     LQVDHTLSSVFYQGDSARSLQSDHFRMGLPTAISIQADWNFHKGFFANASIVRGFGHG
                     SRQGVVRPDIYSLAVRYESEWLEASIPVSLIYYGNWRPRIGFAVRTGYFFFGGDALGG
                     LLGLHDLQAVDFYAGIHFFITR"
     gene            3457..3885
                     /locus_tag="VGM24_11510"
     CDS             3457..3885
                     /locus_tag="VGM24_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY4156052.1"
                     /translation="MESPNKTTITVEATINAPAEKVWQLFTLPAHITQWNSPSGEWHT
                     PRAENDLRPGGKFLFRMEAKDGSAGFDFAGIYDEVNTNRQIAYTIGDGRKVKITFAGD
                     GNKTKVVETFEAETVHSIEMQRGGWQAILDNFKRHAEENK"
     gene            4346..4762
                     /locus_tag="VGM24_11515"
     CDS             4346..4762
                     /locus_tag="VGM24_11515"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY4156053.1"
                     /translation="MEKMRFDAMIRQDTITLSRLLANDLIFIHTNGVVDSKSSLLKSI
                     AARTVIYQFILPEKSTTRVEGNFGWVYGKANIRFKVASINMIIDQYISFINFYRLIHD
                     QWLLVACENAKINTDIPDFGKPLQPQVKKSIQPIIY"
     gene            complement(4800..>5276)
                     /locus_tag="VGM24_11520"
     CDS             complement(4800..>5276)
                     /locus_tag="VGM24_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156054.1"
                     /translation="INYPEQFDMSLQVNIDSLAPVYVRSRNYQQDSLETRMEYKKIFD
                     YGGPDVFDNFGAGPGVSSGIDLDLLFNGRKNRRMLAFQSRLLYDERENYVDHRFSRSL
                     VKRITGLEPPALDSFMRQYRPSYEFIQSFYNDYEYYEYILRSSKYFLLVWKQEHPD"
BASE COUNT         1492 a         1235 c         1192 g         1357 t
ORIGIN      
        1 ggaacccaat aaagagatga ggaaaaaatc ggtggagctt ttaaaccatt atccaaaata
       61 taccacggtc gacggcaaag ctgaaaatac aaaccttcct gccggcagtg tggatgcggt
      121 ggttgccggt caggcatttc attggtttga tgcggagaaa gcaaaggccg aatttaaaag
      181 gattttgaaa cctgccgggc tggtcgtact gatttggaat gaaagaagaa ccgtcacttc
      241 cttcgaaaat gaatacgacg acctcatccg gaagcatgga aaggattacg taaaagtgga
      301 tcaccggaat atccgggata cggatataga aattttcttc tatccggcaa tctgcgaact
      361 taacgttttc gataaccagc agacccttga cctggacgga ttaaaaggcc ggttattgtc
      421 ttcctcctat atgccggcaa ggggcgaatc aggatacccg gacatgatcc gggatctcga
      481 aaaattgttt gagaagcaca aagaaaataa tcggatcaca ataaattatg ataccaaatt
      541 gtatgccggc cggttccaat aacccggatt gatctggccg aaagagcagg tcgacggaaa
      601 ttgcaatttc cctttatttc tccaactttg taaaaatgac ggactctcca gacataagat
      661 cgctaccggg tgcttatcag cttttcctgc ccgagcaggt agccgctatg ctgcacctga
      721 cccaggggga tggcaaggcg ctcgtcatca tccacgaaaa taaaatgcag cttgaatggc
      781 tcgcacagaa aataggagag caatatcatt cactatacaa tgcatttata gccggcaaag
      841 caagactgaa acggaaaaaa cggagggtgc tgatcatcct gctattcgtc gccctggcag
      901 ccatcttcaa tggccctctg gccgggatta tgcaggtcga caggctctat ataaaggctt
      961 ttatttttac ccttggcatc atatgctgta tgcaactgct caaatacctt gcgatgggaa
     1021 tggcgggttc cgccaggggt gttgacaccg agctctctgt tctggagctg aaagaattaa
     1081 tcctcaaaga cttttcgccg gaagaatagc aacctggccc gtgggccccg gctatttttt
     1141 taccggcacc tccaggctga tcgttatcat gtctcccaca gacccgccct ttttgcccac
     1201 tttataatct tcgcggttta tggtaaactg tcctttgaaa atgcccgtac ttccggagga
     1261 ggtaaaagta aatgggattt caaccggccg ggtggttccc ttcagggtta agtcacccga
     1321 aaccttatat cccgcagacg ttttttcaat tttccttgat ttaaaactga tcgttggata
     1381 cttgtccgta tttaaccaca tttccttgtt tcgaaggtcg ctgttgcgca gggaaattcc
     1441 ggtgcttact gttttggcct ctatgctggc tgaaagagcg ctttcccccg gttccttttc
     1501 attaaactga atggttgcct ttaatcccga aaagctgccg tgcacggtcc caaaagggcc
     1561 ttttacagta aagttgattt tagctttagc ctcatcagca ttccaatgct ccggcgggat
     1621 caatccgatc aggctgaaaa gggagatcag taatatcgat ttcatatttg tttgttgtta
     1681 actatttaac gggtaatgaa gaaatgaata ccggcataaa aatcaacggc ctgcagatca
     1741 tgtaacccca ggagtccgcc cagtgcgtcg ccaccaaaga aaaaataacc ggtccgcacg
     1801 gcgaaaccga tgcggggcct ccaattgcca taatatatca gggagaccgg aatactggcc
     1861 tccagccatt cactctcgta tcgtactgcc agggaatata tatccggacg aacgaccccc
     1921 tgtctgcttc catggccaaa tcccctgacg atgctggcat tggcaaaaaa gcctttatgg
     1981 aaattccagt ctgcctggat gctgatcgct gtcggaagac ccatccggaa atgatccgat
     2041 tgtaatgacc tggcagaatc accctggtag aaaacggaac tcagggtatg atcaacctgt
     2101 agattatttg taaagtctgc gttgtgccag ttcgcaaaat tggcgctatc cgcctgaaga
     2161 tggtaagcgg cggacactct gttaaaattg atcgcaccga catcgataaa ggacaatccc
     2221 aaccggtaca catatttact ttcctccggt tcataccatt ttttgcttac cggtgcggcg
     2281 gcggctgttt cccttaaccg ttcatagata atgcctatat cgcccccgag tccgtgtcca
     2341 tgatttaaat cctggtagtt tttgattttt ctataggaat catagtcggt tctgccataa
     2401 tccagattgg aatgggcgaa gagaagctgc gttgtgtcgg agacccggta ggtcaggctg
     2461 gtatttttaa cataggccgc agcaacgccc tgtaaatatt tcagactgat ccccccggtt
     2521 acttcgcggt taccgttctt ccagatgacc cgggcataat gaaatccgta ttctaaccag
     2581 ttcatcccgt ttatccgggt tttatcgtcc gtaaatgtct tattccacag atccttattc
     2641 cttaaataat caaaggcgct ttgtgcgaga tggcccgtaa aatcattgat gtttgcataa
     2701 gacctggaaa ccagcgtaaa cccgataacc tgcgaccggc cgatcttcgc gtgaaaagag
     2761 ggtcccagca tttcacctga taaggtaaag ttatagcgct tgtctggatt tccgggatca
     2821 aaacgcgtat aaaatttatc ctcgtgtttt attccgttaa taatggatct aaaacccagc
     2881 accggaatag ttcctttggg tatatacaga aaattattgt cgaaaacggt tcccagggac
     2941 agcacattca catcccattt caatttagaa tccgctattg cgcccgggtt gagcaacgct
     3001 gcctgaatac cggcataatt gctgcttctt atcccgatat tttgttgcgc ctgtatgaac
     3061 aggaagttat gaacgagcag catgactgag actatttttc tcattcaact ggatttatgc
     3121 agtggccgga taaaaagatt gccgtcatca gtcagagggg aaagggctca gggggcctgg
     3181 acgaaaggga ataaatatat aaacgggaaa ttggctatcc gggttgtacg cgtccctaaa
     3241 aattaaattt aatatcgcgg gagtatcgca ttccctttcc ctggacatct tatacagcac
     3301 ctggagtata tgcaaagctt tgccgcaatc acccccttag attgtccggc tcagcacccg
     3361 gaaatataaa tcgcatttac catctttgaa gagcaatgaa atcgaaaaaa cattttcaaa
     3421 cttcttatca aatcgtaaca tctaaaaaca aataaaatgg aatctccaaa caaaacgacg
     3481 atcacggttg aagcgaccat caatgcaccg gcagaaaagg tctggcaact ctttaccttg
     3541 ccggctcata tcacgcaatg gaacagtcct tccggcgaat ggcatacgcc aagagcagaa
     3601 aacgacctgc ggccgggagg aaaatttctt tttcgcatgg aagcaaagga tggtagcgcc
     3661 ggatttgatt ttgccggtat ttatgacgaa gtgaatacca acaggcaaat tgcctatacc
     3721 atcggagatg gaagaaaagt caaaattact tttgcaggcg atggcaacaa aaccaaagtg
     3781 gtagaaacct ttgaagccga aacggtccat tccatagaaa tgcagcgtgg cggatggcag
     3841 gccatactgg acaatttcaa aaggcacgcg gaggaaaata aataatataa gggcctgtag
     3901 aaacaggccc cggatgacat cttaacgtgt cacatgagaa tagtctttaa acgagataaa
     3961 ctcaggcaaa aatcaggtat ggccggaacg ggtactccgg cgattttaag aattttcact
     4021 aagaccgtct ttcaggcaaa aaagactaag caataagaaa atattttttt acgccggata
     4081 caaatcagac agaggccgga cccggccggt atcctggcag gagtccttca ggcctgccgt
     4141 tttgttcaaa tattacatag gaaaacaaaa agaattccga catttgaccc atgtcttcgc
     4201 ctatcgaaac cgcaaacccc ctattttgaa ctgaatgaat ggaaagtcgc cattactgag
     4261 tcttatatgg tccattatta tattcctgac ggaaactgcc agcgcacagg tgcccttatc
     4321 aacacccgat caaaggttaa tctcgatgga gaaaatgcgt ttcgatgcca tgatccggca
     4381 ggatacgatc acgctttcca ggcttttggc aaatgatctt atttttattc ataccaatgg
     4441 ggtggtagac agcaagtcct cccttttaaa aagtatagcc gccaggaccg taatatatca
     4501 gttcatactc cctgaaaaat cgactacccg ggtggagggc aacttcggct gggtttacgg
     4561 gaaagcgaat atccggttta aagtagccag tattaatatg atcattgacc agtatatcag
     4621 ctttattaac ttttaccggc ttatacacga tcagtggctg ctggtggctt gtgaaaatgc
     4681 gaagatcaat acggatattc cggactttgg caaacccctc caaccccagg tcaaaaaaag
     4741 cattcaaccc attatttact gatcatccgc cggcgaaggg tcgggctttg cggatatcct
     4801 taatccggat gttcctgttt ccacaccagg agaaaatatt tactggaccg gagaatatat
     4861 tcgtaatatt cataatcatt gtaaaaggac tgtatgaatt cgtagctggg ccggtattgc
     4921 cgcataaaac tgtcgagcgc aggcggttca aggccggtaa ttctctttac gagcgatctc
     4981 gaaaaacggt gatcgacata attttcgcgt tcatcgtaaa gcaaccggct ctggaaggcc
     5041 agcatccgcc ggtttttccg gccattaaac aagaggtcta aatcaattcc ggaagaaacg
     5101 cccggacccg ctccgaaatt atcaaacacg tccggcccgc cgtagtcgaa tattttttta
     5161 tactccatcc gggtttcaag ggaatcctgc tggtaatttc tggatcttac gtacaccggt
     5221 gccagagagt ctatattcac ctgcaggctc atatcaaatt gctccggata gttaat
//
LOCUS       DASYCU010000571         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4618327, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000571 DASYCU010000000
VERSION     DASYCU010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4618327"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VGM24_11525"
     CDS             <1..801
                     /locus_tag="VGM24_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156055.1"
                     /translation="WLVMLAMTVPVFSMAQSVKKERKGEFYFSWGYNTEWYTHSNLRI
                     KQPGLGNDFTFKNIRGQDHRGWDEQLFTKALTIPQYNYRIGYIFNKKKGLGFEINFDH
                     TKFIFGDNQWVHVKGRVGNKAYDGQVLFKDNGANDSSSYYYLNNGANFLLFNIVKRWH
                     LVANKKQTILIDGLGKAGIGPVVPHVENKFFDQPKNDPHFQVGGWNVGVEGAIRATFF
                     KYLYLEYTNKLDYARYSGLKIYQGTAKQAFGTYEMILNLGLSFPVGKK"
     gene            complement(803..1564)
                     /locus_tag="VGM24_11530"
     CDS             complement(803..1564)
                     /locus_tag="VGM24_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY4156056.1"
                     /translation="MPSTTGDQPVISSRKPLYLEGPQSRGFELRFALRVFGQIIMGNR
                     ALYFVGPCITVFGSARFREDNAYYEAAREFGKRIAAMGFTVMTGGGPGIMEAANRGAF
                     ENGGPSVGCNIRLPFEQLPNRYMHKWITFDYFFVRKGMMLKYSYAFIVMPGGFGTMDE
                     FFSTLTLIQTKSITQFPMVLFGKDFYKPLMDTIELMKREGTISEEDLSLVLFTDDINE
                     AMGHISNYISRNYVIRKKRRRFWWLFEKKFLNPVR"
     gene            1623..>1968
                     /locus_tag="VGM24_11535"
     CDS             1623..>1968
                     /locus_tag="VGM24_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY4156057.1"
                     /translation="MSSLRIVSYNVNGIRSAMKKGLVDWIKTDPADVICIQETKAHKD
                     NVAHQEITGLGYHDYWFSAQKKGYSGVAVFTKILPDHVAYGTGHQVSDEEGRVIQLDF
                     GDIRLINAYFPSS"
BASE COUNT          559 a          478 c          473 g          458 t
ORIGIN      
        1 tggctggtca tgctggcaat gaccgttcct gttttcagca tggcccagtc ggtgaagaaa
       61 gaaagaaaag gagaattcta tttttcctgg ggatacaata cggaatggta cacgcatagc
      121 aatctgcgta tcaaacagcc cggtctggga aatgatttca cttttaagaa tatccgcggg
      181 caggatcacc ggggctggga tgagcaacta tttaccaagg ccctgacgat cccccagtac
      241 aattaccgca tcgggtatat tttcaataaa aagaaaggac tgggatttga aatcaacttc
      301 gaccacacga aattcatttt cggggataac caatgggtac acgtaaaagg aagggtcggg
      361 aataaggcct acgacggaca ggtactcttc aaagacaacg gcgcgaatga cagcagctcc
      421 tactactatc tcaacaacgg agcgaacttt ctcctgttca atatcgtaaa acgctggcac
      481 ctcgtcgcga acaaaaaaca aaccattctg attgacggcc tcggaaaggc aggtatcgga
      541 ccggtggttc cgcatgtgga gaataaattt tttgaccagc cgaaaaacga tccgcacttc
      601 caggtaggcg gctggaatgt gggggtggaa ggcgctatcc gggccacttt ctttaaatac
      661 ctctacctcg agtatacgaa taaactggat tacgcccgct attccgggct aaagatctac
      721 cagggaacgg ccaaacaggc gtttggaacc tatgaaatga tcctgaacct ggggctgagc
      781 tttcccgtag gtaaaaaata aattaacgga ccgggttgag aaattttttt tcaaacagcc
      841 accagaaccg tcttcttttt ttgcggatca cataattcct ggagatgtaa ttgctgatat
      901 gccccatggc ctcattaata tcatcggtga acaagaccag ggaaaggtct tcctcagaaa
      961 tggtcccttc tcttttcatc agctcaatgg tatccatcag gggcttataa aaatccttcc
     1021 cgaacaggac catcgggaac tgggtgatgg atttggtttg aataagggtg agcgtactga
     1081 aaaattcatc catcgttcca aaaccgccgg gcattacgat aaaagcatac gaatatttca
     1141 gcatcatgcc ctttctgaca aaaaaataat cgaacgtgat ccatttgtgc atataccggt
     1201 tgggcagctg ttcaaaaggg aggcggatat tgcagcctac ggaaggcccg ccattttcaa
     1261 acgcgccgcg gttggcagcc tccataatgc ccggaccgcc gccggtcatc accgtaaatc
     1321 ccatcgccgc gatccttttc ccgaattccc tggccgcctc ataataggca ttgtcttccc
     1381 ggaaccgggc agacccaaag accgttatgc agggaccaac aaaatacagg gcccggttac
     1441 ccatgataat ctggccgaaa acgcggagcg cgaaacgcag ctcaaatccc ctgctctggg
     1501 gaccttcgag gtataaaggc tttctggaag aaataaccgg ctgatctcct gttgtactgg
     1561 gcattcgcta ttcctggtta acaaagatta aattgcagtt cgaatttaat gcaattcatc
     1621 atatgtcatc cctccgcatc gtcagttata atgttaacgg catacggtca gccatgaaaa
     1681 aaggattggt ggactggata aaaacagatc cggcagacgt gatctgtatt caggaaacca
     1741 aggcccataa agacaatgtg gcgcaccagg aaatcaccgg tctcggatat cacgattact
     1801 ggttcagcgc acagaaaaaa ggatacagcg gcgtcgccgt gttcactaag atattgcccg
     1861 atcacgtggc ctacggaacc ggtcaccagg tgagtgatga agaggggagg gttatccagc
     1921 ttgactttgg cgatatccgt ctgatcaacg cttatttccc ttccagca
//
LOCUS       DASYCU010000572         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3022623, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000572 DASYCU010000000
VERSION     DASYCU010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022623"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1036
                     /gene="folP"
                     /locus_tag="VGM24_11540"
     CDS             209..1036
                     /gene="folP"
                     /locus_tag="VGM24_11540"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY4156058.1"
                     /translation="MYTFNCKGRLLKWDQPIVMGILNVTPDSFYAGSRLQTPGQLLKE
                     AEKMLQDGAKILDVGGMSSRPAAGEISVSEELSRVIPAVENIGRNFPEAFISVDSYRP
                     EVIEKALAAGACMINDISGGNEFVIPDLAARWRVPYICMHMRGTPQTMQKLTGYEDLT
                     REVLDYFIQRKSDCILRGVKDLIIDPGFGFAKNREQNFQLLKRISVLKMLELPVLAGL
                     SRKSSIYKTLGISPEEALNGTTVMNTLALENGASILRVHDVREAIETIRLWLTYSNA"
     gene            complement(1063..2001)
                     /locus_tag="VGM24_11545"
     CDS             complement(1063..2001)
                     /locus_tag="VGM24_11545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY4156059.1"
                     /translation="MKKINSFLFTLAAVLLMVACNNQGLKKTKSGLSYKIITDGKNPV
                     VRKGQFIKITFSQKLRDSVLFSSASSMPAYIPIDTPVQANYSPIEIFHLLRKGDSAVV
                     IQRGDSIEKKAGQPLPPYIKKQDKIILTMKVVDVFDNEAAVQKDREEALNAFHEKETR
                     EVADYIAKNNIQAQKTEKGTFVKIDNPGDGPAVDSGKQVSVIYTGKLFPSGKVFESND
                     SKDKAPIKFVIGRRSIIPGWDDGLRLFKKGGKGTLYIPAFLAYDMQKGPGNSPNENLI
                     FDIQIVDVTDAPAEKPAMMPNMSLPGKPAPKKTPAK"
     gene            complement(2010..>2675)
                     /locus_tag="VGM24_11550"
     CDS             complement(2010..>2675)
                     /locus_tag="VGM24_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY4156060.1"
                     /translation="LIDHHQQPQEEMFAYGISDVGKSSTCEMIFDFIIASGNGDKIHL
                     AVAECLYAGVMTDTGSFRFSSATASVHRMVAALKDTGLDHSKIHENLFDNFLENRLRF
                     TGHALLNRLEIFYEYNTALISIPWKDLVKYQIRTGDTEGLVNFPLTIQGIKFAALIID
                     RDEEIKCSFRSKGGFDVNRYARKYFEGGGHMNAAGGRSSETLEQTAHKFRASLKENAL
                     QLQ"
BASE COUNT          646 a          627 c          677 g          725 t
ORIGIN      
        1 cagggtccga acccgcctgg ccttgatgaa caattggtcg agaatggaaa tcattcgcaa
       61 tacgcgccag gaagtgccat agtccaatgt ttttttaata aagatttccc ggcaggaacc
      121 tactgctttt tcatactgac ggagcgtatc tttcatgctc aaaagtaata tttagtattg
      181 atcggcctaa atttacggat tcaaagccat gtatacattc aattgcaaag gccggttgct
      241 gaaatgggat cagcccatcg ttatgggaat tctcaatgtt acccccgatt ccttttacgc
      301 cggaagtcgt ttgcaaacac cgggtcagct gttaaaagaa gcggaaaaaa tgctgcagga
      361 cggggcaaag atcctcgatg tgggcggaat gagctccaga cctgcggctg gcgaaatttc
      421 cgtttcagaa gaattgagcc gggtgatccc tgcggttgaa aatataggaa ggaattttcc
      481 ggaagcgttt atatcggtgg acagctaccg gcccgaagtg attgaaaaag cactggcggc
      541 cggtgcgtgc atgatcaatg atatcagcgg cggcaatgag ttcgtcattc cggatctggc
      601 tgcccggtgg agggtcccct acatctgcat gcacatgaga ggcactccgc aaacgatgca
      661 gaaactgacc ggatacgaag acctgacccg ggaagtactg gattatttca tacagcggaa
      721 atcggactgt atacttcggg gcgttaagga tctgatcatt gacccgggtt ttggttttgc
      781 caaaaacagg gaacagaatt ttcaactgct gaaacgaatt tccgttttga aaatgctgga
      841 actgccggtt ctggcaggat tatccaggaa gtccagcatt tataaaacat tagggatttc
      901 cccggaggag gcgttgaacg gcactacggt aatgaacacc ctggcgcttg aaaacggggc
      961 ttccatttta agggtgcacg atgtgaggga ggccatagaa acgatccggt tatggctgac
     1021 ctactcaaac gcgtaagcca tccacaaccg gacctgttcc ctttatttgg cgggcgtctt
     1081 tttgggtgcc ggtttgccgg gaaggctcat attcggcatc atcgcgggct tttcagcggg
     1141 cgcatcggta acgtctacga tctgtatatc gaaaatcagg ttttcatttg gcgaattgcc
     1201 gggacccttt tgcatatcat aagccaggaa ggcgggaatg tacaaggtgc cttttccgcc
     1261 ttttttgaat aaacggagac catcatccca tccggggata atggatcttc tcccgatcac
     1321 aaatttgatg ggggctttgt ctttgctgtc gttggattcg aatacttttc cggaagggaa
     1381 taacttaccg gtatatataa ccgaaacctg tttcccggag tcaactgccg gtccgtctcc
     1441 cgggttatca atcttcacaa aagttccttt ttccgttttc tgggcctgga tattgttctt
     1501 ggcaatataa tctgccactt ccctggtctc tttttcgtgg aatgcattca gggcctcttc
     1561 ccggtccttc tgcacggctg cttcgttatc aaaaacatcc accactttca tggtaaggat
     1621 aatcttgtcc tgtttcttta tatagggagg aaggggctgc cccgcttttt tctcgatgct
     1681 gtctccgcgc tggatcacta cggcactgtc gcccttccgc agcagatgaa atatttcgat
     1741 cgggctgtag ttggcctgaa cgggggtgtc tatgggaata taggcaggca tgctgctggc
     1801 cgagctgaac aaaacggaat cccgcagttt ctgcgaaaat gtgattttga tgaattgccc
     1861 tttcctgaca acagggtttt tcccatcggt gatgatcttg taggacaacc cggatttggt
     1921 ctttttcagc ccctggttat tacaggcgac catcagcagg acggctgcca gggtaaaaag
     1981 gaaagaattg atttttttca ttttcagttt tattgtaatt gtaaagcgtt ttcttttagt
     2041 gaagcccgga acttgtgtgc ggtctgttcc agggtctcgc tgcttcttcc tccggcagcg
     2101 ttcatatggc cgcccccctc aaaatatttg cgggcatacc ggttaacatc aaaccctccc
     2161 ttgcttctga acgaacattt gatttcttcg tcccggtcaa tgatgagcgc tgcaaatttg
     2221 atcccctgga tcgttagcgg gaaattgacc aggccttccg tatcgcctgt ccggatctga
     2281 tattttacca gatccttcca gggaatggaa atcagggccg tgttgtattc gtagaaaatc
     2341 tcgagacggt tcaacagggc atggccggta aaccgcaaac ggttctccag aaaattatca
     2401 aagaggttct cgtgaatttt ggaatgatcc aggccggtat ccttcaatgc ggccaccatg
     2461 cggtgaacgg aggcggtggc cgaactgaaa cgaaatgatc cggtatccgt catcacgccg
     2521 gcatacaaac attccgcaac ggcaagatga attttatctc cgtttccgga agcgataatg
     2581 aaatcaaaaa tcatttcgca ggtggaactt ttgccgacat cgctgatgcc ataagcaaac
     2641 atttcttcct ggggctgctg atgatgatcg atgag
//
LOCUS       DASYCU010000573         6417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1433343, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000573 DASYCU010000000
VERSION     DASYCU010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433343"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VGM24_11555"
     CDS             complement(<1..685)
                     /locus_tag="VGM24_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4156061.1"
                     /translation="MQTASKKVIRGWAMYDWANSVYSLVITTTFFPIYFNAVTKKQFG
                     SEVIPFLGRKFINSSLYDYCLAAAYLFIALLYPVLTSIADTRGNKKAFMKFFCYMGAG
                     SCSLLFFFNGSNLWLGILCCMVAAMGYIGSVVFYNAFLPEIAAPADRDRVSAKGFSYG
                     YIGSVILQVIGFILVVTITSDPFLAPRITFLLVGLWWAGFAQITFSALPETRLSKPVS
                     RGVISEGFSE"
     gene            complement(703..1500)
                     /locus_tag="VGM24_11560"
     CDS             complement(703..1500)
                     /locus_tag="VGM24_11560"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY4156062.1"
                     /translation="MSLIQAIVIAIIEGLTEFLPISSTAHMKFANPLIGVTENAFTDL
                     FEIVIQLAAILSVVVLYYRQFFDFKKIGFYFKLIIALIPAIIAGVLLKKHIDSLLGNI
                     VFIAVVMIAGGIVLLFIDKLFSKPAVHSQDSVTNKNAFVIGCYQVLSILFPGLSRSAA
                     TIIGGLAQKLDRRTAAEFSFFLAVPTMLAASVKSCWDVHKDHPEVFSTDSMTLLAIGS
                     LVAFIVALIAIRFFITYLQRHGFKIFGYYRILAGSLILLLVWKGIIH"
     gene            complement(1497..1769)
                     /locus_tag="VGM24_11565"
     CDS             complement(1497..1769)
                     /locus_tag="VGM24_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HEY4156063.1"
                     /translation="MAKQIKTTPQTRQYVPIFGKENYRWMLIGLAAILLGLLLMIGGK
                     SKDPNVFDPSQVYSFRRITVAPILILGGLALEIVGIFKKSKARKPQ"
     gene            complement(1799..2671)
                     /locus_tag="VGM24_11570"
     CDS             complement(1799..2671)
                     /locus_tag="VGM24_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY4156064.1"
                     /translation="MANTRKTSGKRFTPSYIMAIIGVSLVLFLLGILGWIIINANKLG
                     QYFKENIAMNVYIRENVSAKDSATLVNYISAQPYVKSYEYVTKDLAKQKYLQDGNKDW
                     GTLLDRNPLPASIDFRINKEYASTDSLSKIKNELLKNIAVSDVLYPQSLVTNLNNIIN
                     KISIILLSVIVIISVLVIFLIDNTIRLAMFSNRFLIKTMQMVGATRWFISRPMDLRAF
                     LNGLISGLLAVGGLVAVIVFAENQLPYLKALRDYPMLLVLFVVIILLGIFISLISTHR
                     SVLKYLKMKLDELY"
     gene            2758..5637
                     /locus_tag="VGM24_11575"
     CDS             2758..5637
                     /locus_tag="VGM24_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY4156065.1"
                     /translation="MEYSFSGLEKKWQEKWTADQVYRVSNNSPKPKYYVLDMFPYPSG
                     TGLHVGHPLGYIASDIFARYKRLKGFNVLHPMGYDAFGLPAEQYALDHGVHPADSTRQ
                     NIKNFRDQLDRIGFSFDWSRQLNTSAPGFYKWTQWIFLQLFNSWYNRAEKKAVSIQSL
                     IQAFETQGSSIVSGYRHLPDFSAAEWKGYPVKQQQDILMEYRLAYCGYGEVNWCEALG
                     TVLANDEVVNGVSERGGYLVVKKKLRQWYLRITEYADRLLEGLEKVEFSEAMKEMQAN
                     WIGKSYGAEIEFEIAKDADQPGNLPNLRVYTTRPDTLFGVDFMVIAPEHELVSQLSTP
                     SRKEAVEAYIAYVNSRSERERMAEKKITGCFTGSYVLNPFNGRRIPVWISEYVLAGYG
                     TGAIMAVPCGDERDYKFARHFEIPVTNIIGKYFTGEEANPTKEALLENSDFLNGIPMK
                     DAIKIVTDRLESMGIGNRQVNYKMRDAAFSRQRYWGEPFPIVWKNGIAFPLDEKELPL
                     LLPEVRNYKPGPDGEGPLANIPEWTSRHLETSTMPGYAGSSWYFLRYMDPDNTREFCD
                     RKASDYWGQVDLYIGGTEHAVGHLLYSRMWTKFLYDLGYIGFDEPFRKLLNQGMIQGS
                     SRFVYRIEIKGPDQVESVLSQSPLFVSSGIKNLPSRQFSDAVREALSRIFPGHQIDEN
                     TILISAIHVDVNLVDGLELDIPSFKSWRPEYAQAEFILENGKYICGSETEKMSKSKFN
                     TVDPTGLVEKYGADTFRMYEMFLGPVEQGKPWDTKGIEGVHRFLRKLWRLFYDESKGK
                     VWTNDPASAAEFKILHKTIRKMDEDTERLSFNTAVSAMMIAVNELGDLKCHKKEVLEK
                     ILILLTPYAPHICEELWHRLGYETTILDAPYPVADQQYLVESEKRYPVAINGKTRTEL
                     NVRLDASQQEVEALVMADETVRKWLDGKSPKKLIYVRNKMINIVV"
     gene            complement(5669..>6417)
                     /locus_tag="VGM24_11580"
     CDS             complement(5669..>6417)
                     /locus_tag="VGM24_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156066.1"
                     /translation="VADFNCPGEGVMKLDGRSSLRDDEQRSKKELHLLKGPNQPIFPD
                     EWDFVMDNYAYSVSNPRIIDSIEQIALGPAPVPGVPEEAPPAPVRQETAAPLQEKEDP
                     LPEKSSLPTVAKQEPVQASPAVPVATQTNEQKFVSRARILQTVIPVKGDSIELRFYDN
                     AEIDGDSIAVFLNGKMLREHILLSDRAYSFKISVNDLAADNELVMVAENLGTIPPNTS
                     LMEAIVGDKQYEAHLQSTEGTSALVRLVKP"
BASE COUNT         1740 a         1515 c         1651 g         1511 t
ORIGIN      
        1 tttcagaaaa tccttccgag ataacccccc tgctcaccgg ttttgataac ctggtctccg
       61 gaagcgccga aaacgtgatc tgggcaaagc cggcccacca caggcccacc agcaaaaagg
      121 taatccgggg cgccaggaaa ggatcactcg taatggtcac gaccaatata aatccgatca
      181 cctggaggat gacgcttccg atatagccat aggaaaatcc tttggcactc acccggtcgc
      241 ggtcggccgg agcagcaatc tccggaagga aggcattgta aaataccacg ctgccgatat
      301 atcccatggc agccaccata cagcataaaa tacccagcca gagattggac ccattaaaga
      361 aaaaaagcag tgaacagctt ccagcgccca tataacagaa gaatttcata aaggctttct
      421 tattgccccg ggtatcggca atggaggtga gcaccgggta aagcaaagcg ataaaaagat
      481 aagcagccgc aaggcagtaa tcgtacaagg aggaattgat aaatttccgc cccaggaaag
      541 gaatcacttc actcccgaat tgttttttgg tgaccgcatt aaaatagatg ggaaagaaag
      601 tggtcgtgat aacgagactg taaacggaat tagcccagtc gtacattgcc catccgcgaa
      661 ttactttttt ggaggctgtc tgcataacct tcagaggctt ttttaatgga taattccctt
      721 ccacaccagc aacaggataa gagagccggc caggatacga tagtaaccga atattttaaa
      781 cccgtgcctt tgcaggtaag tgatgaaaaa ccgtatggct atcaaagcta cgataaaggc
      841 tacgagactg ccgatagcca gcagggtcat actgtccgtt gaaaaaacct ccggatggtc
      901 cttatgaaca tcccagcagc ttttaacgga agccgccagc atggtgggta cggcgagaaa
      961 gaaagaaaat tccgcggccg tgcggcggtc gagcttttgg gcaagccctc cgatgatagt
     1021 agcggcactc cggctgagcc cgggaaaaag gatcgataaa acctggtagc atccgatgac
     1081 aaaagcattt ttatttgtca cggaatcctg gctgtgaacc gccggtttgc tgaacagttt
     1141 atctataaat aagaggacaa tacctccggc tatcattacg accgcgataa acacgatatt
     1201 tcccaacagg gaatcgatgt gttttttaag caatacgccg gcgatgatgg cgggtatcag
     1261 ggctatgatc agcttgaaat aaaaaccgat ctttttaaaa tcgaaaaatt gccggtaata
     1321 aagcactaca accgacagga tcgcggccaa ctggatcaca atctcgaaca ggtccgtaaa
     1381 tgcattttca gtcaccccga tgagcgggtt ggcaaacttc atgtgcgccg tggatgaaat
     1441 aggcaggaat tccgtaagcc cttcgatgat ggctatgaca atggcttgaa taaggctcat
     1501 tgaggtttcc gggctttcga ttttttaaaa atgcccacaa tttcaagtgc caggccgcca
     1561 agaatgagga tcggtgcaac ggtaatccgc ctgaagctgt atacctggga agggtcaaac
     1621 acattcggat ctttgctttt tccaccgatc atcagcaaca ggccgaggag aatggcagcc
     1681 agcccaatga gcatccagcg gtaattttcc ttaccgaata tcggaacata ttgtctggtc
     1741 tggggagtgg tctttatttg ttttgccata aaaggaaata ggttgcaatg tactaaaatc
     1801 aatataactc atccagcttc atcttcaggt atttgagcac gcttcggtgg gtgctgatca
     1861 gggaaataaa aatacccagc agaataatca ccacaaaaag caccaataac atcgggtaat
     1921 cccgcagggc tttcagatag ggcagctgat tttctgcaaa aacgattacg gcaaccagtc
     1981 cgcccacggc cagcaatccg ctgatcagtc cattgagaaa agcccttaag tccatgggcc
     2041 gggaaatgaa ccagcgggtc gctccaacca tctgcatggt cttgatcaga aagcggttgc
     2101 tgaacatggc cagccggatg gtattgtcta tcaggaaaat gacgagcacc gaaatgataa
     2161 cgattaccga aagcaggatg atgctgatct tattgataat attgttcagg ttggtgacca
     2221 gggactgcgg ataaagcaca tcgctcaccg caatgttctt aagcagttca ttcttgattt
     2281 tggacaggga atcggtggaa gcatattcct tgtttatccg gaaatctatg ctggccggga
     2341 ggggattccg gtccagcagg gttccccagt ctttgttgcc atcctgcagg tatttttgct
     2401 tggccagatc cttggtaaca tactcgtatg atttaacata aggctgggcc gaaatataat
     2461 tcaccagggt ggcgctgtcc ttcgcggaaa cattctcccg gatgtacaca ttcattgcaa
     2521 tattctcttt aaaatactgt ccgagtttat tggcattgat aatgatccat cccagaatgc
     2581 ccagcaggaa aagcaccagc gacacgccaa tgatggccat aatataggaa ggcgtgaacc
     2641 gtttgcctga tgttttccgg gtattcgcca tagtggaatt atttctgttt tatctcacaa
     2701 tagtaaggga caaaaataga agatttaccc gcacgatgcg tatttttgtt ggttcatatg
     2761 gagtattcat tcagcggact ggaaaagaaa tggcaggaaa aatggacagc cgatcaggta
     2821 taccgggtaa gcaacaacag tccgaaacca aaatattatg tactggacat gttcccttat
     2881 cccagcggga ccggtttgca tgtcggccat ccgctgggtt atatcgccag tgatattttc
     2941 gcccgctata agaggctgaa aggctttaat gtgctccatc cgatgggata tgatgctttt
     3001 ggcctgcctg ctgaacaata tgcgctggat cacggagtac acccggccga cagtacccgc
     3061 cagaatatca aaaatttcag ggatcagctg gatagaatcg gtttttcctt tgactggagc
     3121 cggcagttga acacctcggc gcccggtttc tataaatgga cccagtggat ctttttacag
     3181 ttgtttaaca gttggtataa ccgggcggag aaaaaagcgg tttcgatcca atcgcttata
     3241 caggcttttg aaacgcaggg gagttccatt gtttccggat accggcatct tccggatttc
     3301 agcgctgccg aatggaaggg atacccggta aaacaacagc aggatatcct gatggaatac
     3361 cgccttgcct actgtggata tggcgaggtg aactggtgcg aagccctggg aaccgttctg
     3421 gccaatgatg aagtggtcaa cggggttagt gaacgcgggg gatacctggt ggtaaaaaag
     3481 aaattgagac aatggtacct gcgcatcacc gaatatgcgg accgcctgct ggaaggcctg
     3541 gagaaagtgg aattcagtga agccatgaag gagatgcagg caaactggat cggcaaaagt
     3601 tatggggccg agatcgaatt tgaaatagca aaggacgcag accaacccgg caacctgcct
     3661 aacctaagag tgtacacgac ccggcccgat accctgttcg gagtcgattt tatggtcatt
     3721 gcgccggaac atgagctcgt ttcccagctc agcacccctt cccggaaaga ggctgtggaa
     3781 gcctatatcg cctatgtgaa cagccgcagc gaaagggaaa ggatggcaga aaaaaagatt
     3841 acgggctgct tcacgggatc atatgtattg aacccgttta acggccgccg cattcccgtt
     3901 tggatctccg aatatgtgct tgccggatac ggaacagggg ccatcatggc ggttccctgc
     3961 ggggatgaac gggattataa attcgcgagg cattttgaga taccggttac caatatcatc
     4021 ggaaaatatt ttacgggaga ggaagccaac ccgaccaaag aagcgttgct ggaaaacagt
     4081 gattttctga acgggatccc gatgaaggat gccataaaga tcgtaaccga ccggctggag
     4141 tccatgggca tcggcaaccg ccaggtcaac tataaaatgc gggacgctgc gtttagccgt
     4201 cagcgttatt ggggcgagcc cttcccgatc gtttggaaga acgggattgc ctttcccctg
     4261 gatgaaaagg aactgcctct cctattgccg gaggtcagaa actataaacc cggaccggac
     4321 ggggaaggtc cgctagcaaa cataccggaa tggacttccc ggcatctcga aaccagcacg
     4381 atgccagggt atgcgggcag cagctggtat ttccttcgct atatggatcc ggacaatacc
     4441 cgggagttct gcgaccgcaa ggcatcggat tactggggac aggttgatct ttatatcggg
     4501 ggaaccgagc atgcggtagg tcatttgctg tacagccgga tgtggaccaa atttctgtac
     4561 gacctgggct atatcggatt tgacgaaccg ttccgcaaac tgctgaatca ggggatgatc
     4621 caggggagca gccggtttgt ttaccggatc gaaataaaag gcccggacca ggttgaatcc
     4681 gtgctgagcc aatcgcccct gtttgtgtct tccggaataa agaacctgcc ttcccggcaa
     4741 ttttccgatg ccgtccggga ggcattgagc aggatctttc cgggtcatca gatagatgaa
     4801 aatacgattt tgatttctgc cattcacgtc gacgtgaacc tggtggatgg cctggagctc
     4861 gatatcccct cctttaaatc ctggaggccc gaatatgcac aggctgaatt tatcctggag
     4921 aacgggaaat acatctgcgg ttccgaaacg gagaaaatgt ccaaatcgaa attcaatacg
     4981 gttgatccga ccgggctggt tgaaaaatac ggagccgata cttttcgaat gtatgaaatg
     5041 ttccttggac cggttgagca gggcaaaccc tgggacacca aaggaattga aggggtgcac
     5101 cggtttctgc gcaagctctg gcgtttgttt tatgacgaat ccaaaggaaa ggtctggacc
     5161 aatgatcctg catccgcggc agaattcaaa atcctgcaca aaacgatccg gaagatggac
     5221 gaggatacgg aaagactctc tttcaataca gccgtcagcg ccatgatgat agcggtgaat
     5281 gaactcgggg atctgaaatg ccataaaaag gaagtgctgg aaaaaatcct catccttctt
     5341 accccttatg cacctcatat ttgtgaggaa ctctggcatc gcctgggtta tgaaaccacg
     5401 atcctggatg cgccttaccc cgttgccgat cagcaatacc tggtcgaatc ggagaaacga
     5461 tacccggttg cgatcaatgg gaagaccagg actgaactga atgtgcgcct ggatgccagt
     5521 cagcaggagg tggaagccct tgttatggct gatgaaacgg tcagaaaatg gctggatggt
     5581 aaatcaccca aaaaactgat ctatgtgaga aataagatga tcaatatagt tgtatgatca
     5641 cttccctttc cggaatacag gcgcccggtc agggcttcac taaccgcacc agggcactgg
     5701 tcccttccgt actctgaaga tgggcttcat attgtttatc tcccacaatc gcctccatca
     5761 gggaggtgtt tggcggaatg gttccgaggt tttctgccac catcacgagt tcattgtctg
     5821 ctgccaggtc atttacggaa atcttaaagc tataagcccg gtcggaaagc aggatatgct
     5881 cccgaagcat ttttccgttt aaaaagactg caatcgaatc cccgtcgatt tccgcattat
     5941 cataaaaccg gagttcaatg gaatcccctt ttaccggaat aacggtctgt aatatcctgg
     6001 ctctgctgac gaatttttgt tcgttcgttt gtgtggccac cggaacggct ggagacgcct
     6061 gcaccggctc ctgttttgct actgttggaa gagaagattt ctccggtaaa ggatcttctt
     6121 tctcctgaag aggagcggcg gtttcctgcc ggactggtgc cggcggagct tcttccggaa
     6181 cgcctggaac cggggcagga cccaaagcga tttgttcaat gctatcgata atgcggggat
     6241 tgctgacgct gtaggcatag ttgtccatca caaaatccca ttcatccggg aaaatgggct
     6301 ggttcgggcc tttcaggagg tgcaattcct tttttgatct ttgttcatcg tccctcaggg
     6361 aacttcttcc atccagtttc atgacgccct cccccgggca attgaaatca gcgacgg
//
LOCUS       DASYCU010000574         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2442471, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000574 DASYCU010000000
VERSION     DASYCU010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442471"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="VGM24_11585"
     CDS             complement(<1..1389)
                     /locus_tag="VGM24_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156067.1"
                     /translation="MHRIFLLPLLLLLFPAPQAGAQIFGGTPTSIRWNQINVLPARII
                     FPRGLEKEARGVAFLVSRLNDSTRTTIGDREHRVNIVFHNLTTISNGYVQLAPFRSEF
                     ELTPSQNSFDLGSLPWYETLAIHEYRHVQQFNNFRVGLSKAFYYLFGEEGLALANNLS
                     VPNWFWEGDAVYQETLVSRQGRGRLPLFLAGYQALWLAGKNYSWMKLRNGSLRDYTPD
                     HYPLGYMLVAYGREKFGPDFWRKTAVDAAAFKSLFYPLQHSVQRHARTSFRTFRQEAL
                     DYFRNQLPDSAYSDANAAFGRRQLHFSADESFPQFTDSTHLIFLKSSYRLPPQFVEMD
                     LKSKKEKRIRFRAVSSDDQFSYRNGQIVYAAYEPDLRWGWRDFSVIRLLNLKTGRDIR
                     ISNRTKYFSPDLSPDGKFIAAVAYSESANCFLDILDSRSGKLIYRLPNPGNLYFTYPK
                     FFSGQELVSAVRN"
     gene            1480..>2971
                     /locus_tag="VGM24_11590"
     CDS             1480..>2971
                     /locus_tag="VGM24_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156068.1"
                     /translation="MEVKSRLFSLSTNLDSLIAAALGFGIIQVFSRHSGIGVSPDSVT
                     YISVARHLLEGNGFRAFDDLPVVDFPAGYPFFLATVSFFTRMDPLQYGAVMNGLLFGL
                     LLYLCGTVMNGFYHTSKWYKRVLLACLVLSPALLEVYSMLWSETLFLLLLILFTIAIK
                     KYLEKPGMKWLVISAALVSLACITRYAAMVLVGIGLFLVFFEGKLSWGKRFRHCIYFG
                     CISVFLLAINLVRNEMVTHLATGERQKSTSSLLNNMAYFGGVLDDWLLIPRDSGLPVV
                     LTLLVLFIFLISMLWCYFRRKAERRYEYIVAATGLFYCLFMLVSASLSRYEQFTSRLL
                     APMFIPLLWCFSFWLPSFVARKKKGFKLAYIAVSLALAAVFLNIQWSADYETYDGVKD
                     AGIPGYFEDPFPQSEIVRFIKKYKPQFRPGYRIYSNAPDAVYLFGGMPARLLPQWAFP
                     NEIQKYYQEKNNYLVWFDSLANPDLLDEKSVIRNKAMVPVKQLADGA"
BASE COUNT          691 a          726 c          786 g          768 t
ORIGIN      
        1 atttcgcacg gcggatacca gttcctgacc ggaaaagaat tttggatagg taaagtacag
       61 gttcccggga ttgggaagcc ggtatattaa tttcccggac cggctgtcga ggatgtccag
      121 aaaacagttc gctgattctg aatacgccac tgccgcgatg aattttccat cgggagaaag
      181 atccggtgaa aaatattttg ttctgttgct gatccggata tccctgccgg ttttcagatt
      241 caacagccgg ataacggaaa agtcgcgcca gccccagcgc agatcgggtt cataggcggc
      301 atagacaatt tgcccgttgc ggtaagagaa ctggtcgtcg gatgaaacgg cccggaaccg
      361 gattcgcttt tctttttttg acttcagatc catttcgaca aactgcgggg gcaaccgata
      421 ggaagacttc agaaaaataa gatgggtcga atcggtgaat tgcgggaatg attcatccgc
      481 agaaaaatgc aattgccggc ggccgaacgc cgcattcgca tccgagtaag cagaatccgg
      541 cagctggttg cggaaataat cgagtgcttc ctgccggaac gtccgaaagg aggttcgcgc
      601 atgcctttga accgagtgct gcagcggata gaaaagggat ttgaacgctg cggcatccac
      661 ggcggtcttt ctccaaaaat caggtccgaa tttttccctt ccgtacgcaa ccaacatata
      721 acccagcgga tagtggtccg gcgtatagtc ccggagagaa ccattgcgga gtttcatcca
      781 ggaatagttt tttcccgcca gccagagggc ctgatatcct gccaggaata acggaagccg
      841 tcctctgccc tgccggctga ccagtgtttc ctgatagacg gcatctcctt cccagaacca
      901 gttgggaacc gaaaggttat tggccagggc cagtccctcc tctccaaaga gataataaaa
      961 agcttttgac aggcccacgc ggaaattgtt gaattgctga acgtgccggt attcatggat
     1021 ggcgagggtt tcgtaccagg gaaggcttcc caggtcaaag ctgttctggg acggagtcag
     1081 ttcaaactca ctgcggaaag gagccagctg cacatatccg ttggaaatgg tggtcaggtt
     1141 atgaaaaaca atattcaccc ggtgttcccg gtcaccgatc gtggtccggg tgctgtcgtt
     1201 aagccggctg accaggaaag ccactccgcg cgcttctttt tccaatccac gggggaaaat
     1261 gatcctggca ggaagcacat tgatctggtt ccagcggatg gaagtaggcg ttccgccgaa
     1321 aatctgtgcg cctgcctgcg gagccggaaa aagaaggagc agcaggggca aaagaaaaat
     1381 ccggtgcatc cgaaatgagt ttctggaacg aaaattgaaa ttaccggata accacgacag
     1441 aaaaaattat gataaatact tttaccttcg gttaacggca tggaagtaaa gtcgcgttta
     1501 ttttctcttt ccaccaacct ggattccctg atcgctgctg cattgggatt cggtatcatc
     1561 caggttttct caaggcacag cgggatcggt gtttcgccgg actcggtaac gtatatcagt
     1621 gtggcccggc atctgctgga gggcaatggt ttccgggcct ttgatgatct accggtagtg
     1681 gacttcccgg caggatatcc tttttttctg gccacggttt ccttttttac ccggatggat
     1741 ccgctgcagt acggagcagt gatgaacggg ctgcttttcg gattgctcct ttatttatgc
     1801 ggaaccgtca tgaatgggtt ttaccatact tcgaaatggt ataaacgcgt tctcctggct
     1861 tgcctggtat tgagccccgc cctactggaa gtatattcca tgctatggtc ggaaacgctt
     1921 tttctcctgc tgctgatctt attcactata gccatcaaga agtacctgga aaagccgggg
     1981 atgaaatggc tggtcatcag tgccgcgctg gtctccctcg cctgcataac ccgctatgcc
     2041 gcgatggtgc tggtcggaat cggcctgttc cttgtttttt ttgaggggaa attatcgtgg
     2101 ggaaaaagat tccgccattg tatttatttc ggatgcattt ccgttttttt gcttgctatc
     2161 aacctggtcc gcaatgaaat ggtcacccac ctcgctaccg gagaacggca gaagagcacc
     2221 agttcgctgc tgaataacat ggcctatttc ggcggcgtcc tggatgattg gcttttgatt
     2281 ccaagagact ccggcctgcc cgtggtgctg accctcctcg tgctttttat ttttctgatc
     2341 agcatgctct ggtgctattt ccggcggaag gcagaacgcc ggtatgaata tattgtggcg
     2401 gcaaccggtc ttttttattg tcttttcatg ctggtctcgg ctagtctttc ccgttatgaa
     2461 cagttcacca gccggttact ggcgccgatg ttcattcccc tgttatggtg tttcagtttc
     2521 tggttgcctt cttttgtggc caggaagaaa aaagggttta agctggcata tattgcagtt
     2581 tcgctggcac tggcggctgt tttcctgaat atccagtggt cggctgacta tgaaacctat
     2641 gatggggtga aagatgccgg tattcccgga tatttcgagg atccctttcc tcaatcggag
     2701 atcgtccggt tcataaaaaa gtacaaaccg caattcaggc ccggataccg gatatactcc
     2761 aatgcacccg atgcggttta cttatttggc ggtatgccgg cgcgccttct tccccaatgg
     2821 gcctttccga acgaaatcca gaaatattac caggaaaaga ataattacct ggtttggttc
     2881 gacagcctcg ccaatccaga cctgctggac gagaaatccg ttatccggaa taaggcgatg
     2941 gtcccggtaa aacaactcgc cgacggagcg g
//
LOCUS       DASYCU010000575         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3459936, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000575 DASYCU010000000
VERSION     DASYCU010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3459936"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..535)
                     /locus_tag="VGM24_11595"
     CDS             complement(2..535)
                     /locus_tag="VGM24_11595"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY4156069.1"
                     /translation="MKGSIWLFCFFLISRISFAQLHPVEKESKISFTIKNFGFNVTGS
                     FSAPSGEIRFDPDNPADARFDVSVSAASINTDNNLRDGHLRGEDYLDTKNYPRIRFVS
                     VRVQPARKKGSYQMTGNLTIRDKTKEISFPFTADPSGTGYLFAGDFKINRKEFGVGGS
                     STVSDNLTVELKVLARQ"
     gene            complement(570..959)
                     /locus_tag="VGM24_11600"
     CDS             complement(570..959)
                     /locus_tag="VGM24_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY4156070.1"
                     /translation="MKLRNLVLWILRLVPAIILLQTLYFKFTGHPQSVKLFTQIGMEP
                     WGRIGSGIVELVAAILILIPRFTGYGAILGLVMMTGALFFHLTKIGVYFDGDPVLFIY
                     AVITFICCAVLIFAYQDRLRGLPRAQR"
     gene            complement(974..1435)
                     /locus_tag="VGM24_11605"
     CDS             complement(974..1435)
                     /locus_tag="VGM24_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HEY4156071.1"
                     /translation="MKKGLLCVLLSISGLIVFSQTDAFREKQYNLEAGVGIKGYDPVT
                     YFSPGRPQKGAKNISFPWHGITYYFSSDRNRETFRANPEKFEPQYGGWCAYAMGHDGS
                     KVEVDPETFKIIKGKLFLFYNRFFTNTLKSWNQDEGNLHARADANWQKFIH"
     gene            complement(1484..2185)
                     /locus_tag="VGM24_11610"
     CDS             complement(1484..2185)
                     /locus_tag="VGM24_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY4156072.1"
                     /translation="MPADDKMLLIRNYDLWCHLSDQDYKDLNIEHHFMEVPKGEYIYF
                     EAYNHNKLYFVKEGYIRIGYIDADGNEKVKEIIQKGELFGQFTLERDNLHGEFAQAYK
                     QDVSLCAFHIDDFEKLLKNRPDLALRYSKQVGAKLRNIENRLLNLLNKDVKTRLIHFF
                     WQLVEQKLGENMPEGFCIPNYLKHEDIANLIGSSRQTVTTLINELETDGILSYNRQQI
                     CFLNVKKLQKLVSVN"
     gene            2272..3078
                     /locus_tag="VGM24_11615"
     CDS             2272..3078
                     /locus_tag="VGM24_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY4156073.1"
                     /translation="MQVPLMAGLSANGEEPEILFWVGCSGSFDQRAQKITRAFTLILQ
                     HIGIRYAILGKEEMCTGDPARRAGNEFLFQMMAYQNIQILNNYHIRKIVTACPHCFNI
                     FKNEYPDLGGHYEVVHHSVFLQQLLDEGKIRLRGEGGFRGKKIVYHDSCYLGRANNIY
                     EAPRKVLEALDAELVEMKRCRSKGLCCGAGGAQFFKEEEKGTTRINFERTREAVDSGA
                     GIIASACPFCNTMLTDGVKHQEKEDQVAVYDLAELIAAELDNGLPASGNQ"
     gene            complement(3080..3772)
                     /locus_tag="VGM24_11620"
     CDS             complement(3080..3772)
                     /locus_tag="VGM24_11620"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEY4156074.1"
                     /translation="MTPKACLSFGNILCLNLGFCKFFMFLSDIEYFPPISFFKASYTN
                     TYIYLDQYEIYRKMSFRNRCLIAGTNGIIALSVPIRQGRNQRVAMNEVRISATEDWQA
                     RHWKSIRSAYNRSPFFDYYLDELEFLYQSKFEMLMDWNLECLHWVKAKLSWPAEIRLT
                     ENPEQGSRPGVWTDYRNLVLPKNYMQQTPVKYRQVFEEKTGFYPNLSILDLLFNAGSA
                     AGDRLKNEGSPG"
BASE COUNT         1068 a          915 c          853 g         1050 t
ORIGIN      
        1 tttactgtct ggcgagtact ttcagttcaa ccgtcaggtt atccgaaacc gtactggaac
       61 cacccacccc aaattccttc cggttaattt taaaatcacc agcaaaaagg tagccagttc
      121 cggaaggatc agcggtaaag ggaaagctga tctctttggt cttatcccgg atagttaaat
      181 ttccggtcat ttgataactt ccctttttcc tcgccggctg aacacgcacc gatacgaagc
      241 ggatgcgcgg atagtttttt gtatccagat aatcttctcc cctcaggtga ccatcccgca
      301 ggttattgtc cgtattaata ctggccgcgc tgacgctcac atcaaaacgg gcatcggcag
      361 gattatcggg gtcgaaccgg atttcccccg acggggcact gaaagaaccc gtcacattga
      421 atccaaaatt tttaatcgta aaactaatct tcgactcctt ttctacggga tgcagttgcg
      481 caaaactgat ccggctgatc agaaaaaaac aaaacagcca tatcgatcct ttcattattt
      541 ttttttaggt tggcaatctg aaccctcact taccgttgtg cgcgcggcaa gccgcgcaaa
      601 cgatcctggt atgcaaaaat aagcaccgca caacagataa aagtaattac agcatagata
      661 aaaagaacgg gatccccgtc aaaataaaca ccgattttgg tcaggtggaa gaataaagcg
      721 ccggtcatca tcaccagccc taggatcgcg ccataccccg tgaaccgcgg gatcaggatc
      781 agtatggcgg caaccagttc cactataccg gaacctatcc ggccccaggg ctccatgccg
      841 atttgagtaa ataactttac agactgggga tggccggtga atttaaaata gagggtctgc
      901 aaaagaatga tggcgggaac caggcgcagg atccaaagaa ccagatttct taatttcata
      961 tagcaaggga gatttaatga atgaattttt gccagttcgc atctgcccgg gcatgcaggt
     1021 taccctcgtc ctgattccag ctcttcaggg tgttcgtaaa aaaacggttg taaaataaaa
     1081 aaagttttcc tttaatgatc ttgaatgttt ccggatcgac ttccactttc gaaccgtcgt
     1141 gacccatcgc ataagcacac catccgccat attggggctc aaacttctcc gggttggcac
     1201 ggaaggtttc acggtttcgg tcagaagaga aataataggt aatcccgtgc cagggaaagg
     1261 aaatattctt cgcccctttt tgcggcctgc caggtgaaaa ataagtgacg gggtcatatc
     1321 ctttaatgcc gaccccggcc tccagattat actgtttttc ccggaaagcg tctgtttggg
     1381 aaaatacaat taacccggaa atagacaata aaacgcaaag gagccctttc ttcataaatc
     1441 aattttatca aaaatagagt tcctttcccc ggcaaattgt cagttaattg acagacacga
     1501 gcttttgtaa ttttttaacg ttgagaaaac agatctgctg gcggttatag gaaagaatgc
     1561 cgtccgtttc cagttcattg atcagggtag tgaccgtctg gcggctggat ccgatgaggt
     1621 tggcaatatc ctcatgcttc aaataattgg ggatgcaaaa tccctccggc atattctctc
     1681 ccagcttctg ctctacgagc tgccagaaaa aatggatcag ccgcgtcttg acgtctttat
     1741 tcagcaggtt caacagccgg ttttcaatat tcctcagttt ggcccccacc tgtttgctgt
     1801 accgaagcgc caggtcgggc cggtttttca ataatttctc gaagtcgtcg atatgaaagg
     1861 cgcagaggct tacatcctgt ttatatgcct gggcaaattc cccgtgaaga ttatctcttt
     1921 ccagcgtaaa ctgtccgaac aattctcctt tctgaatgat ctctttcact ttttcattcc
     1981 cgtctgcatc tatatatccg atccggatat acccttcctt cacgaaatac agtttattat
     2041 gattatatgc ttcaaaataa atgtactccc ctttggggac ttccataaaa tgatgttcaa
     2101 tgttcaggtc tttataatcc tgatcgctta aatggcacca cagatcataa ttccggatca
     2161 ggagcatctt atcatctgcc ggcataatcg atattaagga ttaaaattac cgattcatcc
     2221 cttaactgca ctcaggaaat ttaagctaga tttacggtat aaaatttcta catgcaagta
     2281 ccgctcatgg ccggactttc cgcaaacgga gaagaaccgg agatcctttt ctgggtcggc
     2341 tgttccggta gtttcgatca gcgcgcccag aagatcacgc gggcattcac acttattctt
     2401 cagcatatcg gtatccgtta tgcaatcctg ggaaaggaag aaatgtgtac gggggatcct
     2461 gcccgccggg ccggaaatga atttcttttc cagatgatgg cctatcagaa tatccagata
     2521 ttaaataatt atcatatccg gaagattgtg accgcctgcc cgcactgctt caatattttt
     2581 aagaatgaat atcccgattt gggcggccat tatgaagtcg tgcatcattc tgtttttctt
     2641 cagcaattgc tggatgaagg aaaaatacgg ctccgggggg aaggcggctt caggggaaag
     2701 aagatcgttt atcacgacag ttgctatctc ggccgcgcca ataacatcta cgaggcgccc
     2761 aggaaggtcc tggaggccct cgatgcggaa ctcgttgaaa tgaaacggtg ccgaagcaag
     2821 gggctttgct gcggggccgg aggcgcgcag ttttttaagg aagaagaaaa gggtaccacc
     2881 cggattaatt ttgaaagaac ccgcgaggca gtggatagcg gtgccggcat cattgcctcc
     2941 gcctgtccct tttgcaatac catgctgacc gatggagtga aacatcagga aaaagaagat
     3001 caggtggccg tatatgacct cgcagaactt attgctgcgg agttggacaa tggcctgcct
     3061 gcttccggaa atcaataaat cagccgggag atccctcatt ttttaacctg tccccagccg
     3121 cactgcccgc attaaataac aagtccagga tagataggtt aggataaaat cctgtttttt
     3181 cctcgaagac ctgtcggtat ttcaccgggg tttgctgcat gtaatttttt ggtaagacca
     3241 ggttccggta atccgtccat acacccggcc ggcttccctg ttccgggttt tctgtaagcc
     3301 ggatttcggc cggccaactc aatttcgctt tcacccagtg gaggcattcc aggttccagt
     3361 ccatcagcat ttcaaatttt gactgataga ggaattccag ttcatccagg taataatcaa
     3421 aaaaaggcga ccggttgtag gccgaccgga tggacttcca gtgcctcgcc tgccagtctt
     3481 cagtcgctga aatccgcact tcattcatcg ccacacgctg gttcctgccc tgtcttatcg
     3541 gaacggataa ggcaatgatt ccatttgtgc cggcaatcag gcaacggttc cgaaaactca
     3601 ttttacggta gatttcatat tgatctaaat aaatatatgt gttcgtatac gaagctttaa
     3661 agaagctaat cggcggaaaa tattctatat cagataaaaa cataaaaaat ttgcagaacc
     3721 ctaaattaag acataaaatg tttccaaaac tcagacaggc tttgggagtc atatttatta
     3781 ttatattaaa tgaattatat atttaattgt atatcaatat gttaataaaa ttatttgtac
     3841 taaatatatt ggcctaaaga gcatagtatc ggcccggata attttc
//
LOCUS       DASYCU010000576         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1434098, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000576 DASYCU010000000
VERSION     DASYCU010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434098"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VGM24_11625"
     CDS             <1..773
                     /locus_tag="VGM24_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4156075.1"
                     /translation="PKDYPALNFTEMKIHLIEGHNRLLPQMSEKAGNRAKKDLERMGV
                     TVHLNVRVDSYDGRRVHIKDGEPIDAFTVVWSAGVKSTSFPGLPAEWLERGRIVTDEH
                     CRIPQAGNIFAVGDIALMKTLKYPQGHPGVAQVAIQMGSHVGAHLRELSEGKPVKPFR
                     YFDKGTLATIGRGKAVADLPGNIRFGGQFAWLIWLFVHINYLISFRNKLLVFASWIWN
                     YFTYDKGNRLIIRPFTRKKNPAVAEQARSSHSGSLVAD"
     gene            865..1314
                     /locus_tag="VGM24_11630"
     CDS             865..1314
                     /locus_tag="VGM24_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156076.1"
                     /translation="MKNKLLLLLGIFFVFTGTTAFAKSGDNGIPEPVVSEFNFDFGQA
                     SQVKWEEVNTYYKASFYDQGKILFAFYTADGSFMGSAHNLLSDGLPASLRSKIKTDYS
                     GYWIDELFKFTIADQPGYFIALENADQKITLKTDANQNWRLVKTIKK"
     gene            1339..2196
                     /locus_tag="VGM24_11635"
     CDS             1339..2196
                     /locus_tag="VGM24_11635"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY4156077.1"
                     /translation="MKSISIATALFLSLCSGSFVHAQEGRWRFVRTRDEAPDCSECGL
                     AAVNGKLYLIGGDGEGPKAVQSFNPETLTWTKRAVAPLTIHHFQAVAWQNKIYVLDAF
                     SSGGFPDQVPMGHVYSYDPENNAWEKGGEIPAGRKRAGAAAVAYKNKLYLIAGIQHGH
                     SSGTTNMFDAYDPQTKTWTVLPDAPHIRDHCSAAVINDKLYVVGGRNTSYRDPENKIP
                     FFAKTVLDVDVYDFSTGKWSTHAARLPLGSGGGAVVNLNNVLYYMGGERASSTERNAP
                     RKIPTTWIR"
     gene            complement(2420..2728)
                     /locus_tag="VGM24_11640"
     CDS             complement(2420..2728)
                     /locus_tag="VGM24_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY4156078.1"
                     /translation="MKKITRVWHGITKAEHADEYLKYVEDTGLKEYKTVPGNISTKLL
                     RRIDGDVCHFLTVTEWDSYESIKKFSGPDYQKAKYYPEDKKYLLFFEEDVFHYETFEY
                     "
     gene            3001..>3934
                     /gene="ligD"
                     /locus_tag="VGM24_11645"
     CDS             3001..>3934
                     /gene="ligD"
                     /locus_tag="VGM24_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY4156079.1"
                     /translation="MLATLADRAFDEPGWLYEIKWDGYRAIAYCNRSAVSLMSRNQKS
                     FDDKFYSIREAIIQWNIHAVVDGVITVLNDQGISDFGGLQNWRSEADGELIYYVFDLL
                     WLNGHNLMDLPLLRRRELLKELLPENGPIRLSEHFETSAKEFLESARKLGLEGIIAKK
                     ADSLYQPGDRSRDWLKIKLSRRHEVVIGGYTCNEGSSKAFSSLLVGVFEKGKFRYTGK
                     VGTGFSSRQQKEMLSLFKPLIVKKTAFESEPDINKPSRFRPDPPKAKAVWLKPKLVCE
                     VHYTEITGDGVMRHPSFEAMREDKDAKDVGPEKAL"
BASE COUNT         1035 a          945 c         1041 g          913 t
ORIGIN      
        1 tgccgaagga ttatcccgcg ctgaatttta cggaaatgaa gatccacctg atcgagggac
       61 ataaccgctt attgccgcag atgtctgaaa aagcgggaaa ccgggccaaa aaggatctcg
      121 aaagaatggg cgttaccgtc cacttaaatg tgcgggtgga ttcttatgac ggcagaaggg
      181 ttcatataaa agacggggag cccattgatg ccttcaccgt ggtctggtct gctggggtaa
      241 aaagtaccag tttccccgga ctgccagccg aatggctgga acgaggccgc atcgtcaccg
      301 atgaacactg ccgcataccg caggccggga atattttcgc agtcggggat attgcgctca
      361 tgaaaacctt aaaatatccg cagggtcatc ccggcgtggc gcaggtggcc atccagatgg
      421 gcagtcacgt gggagcgcat ttgcgcgaac tgagtgaagg aaagcccgtg aaaccattcc
      481 gctattttga caagggcacg ctggcgacta tcggacgtgg caaagcggtc gccgatcttc
      541 ctgggaatat ccgttttggt ggccagtttg cctggcttat ctggcttttc gtgcacatca
      601 attatctgat cagcttccgc aacaaactgc tggtttttgc cagctggatc tggaattatt
      661 tcacctacga caagggcaac cgcctgatca tccggccttt tacaagaaaa aagaatccgg
      721 cagtggcaga acaggcccgt tccagtcatt ccggtagttt ggtcgcggat taagccgctt
      781 atgtcgcatt tccctgaatg ggttaaaagt ccggaatact tttgctccgt aattatttaa
      841 tcattcagaa caaaaatgcg aatcatgaaa aacaaattgc tcctcctcct gggaatattt
      901 tttgtattta ccggaacaac cgcctttgca aagagcgggg ataacgggat tcccgaaccc
      961 gtggtttccg aatttaattt tgattttggc caggccagcc aggtaaaatg ggaagaagtg
     1021 aatacctatt acaaggccag tttttacgac caggggaaaa ttttatttgc cttttataca
     1081 gcagacggca gctttatggg atccgcgcac aacctgctct ccgacggact cccggcttcc
     1141 cttcgctcca aaattaaaac ggattacagc ggctactgga tcgatgaatt gtttaaattc
     1201 acgatagccg accaaccggg ttatttcatc gctcttgaaa acgccgatca gaaaatcacg
     1261 ctgaaaacag acgctaatca aaactggcgc ctggttaaga cgattaagaa atgattttca
     1321 ccagttaaaa agcagcatat gaaaagcatc tccattgcca ccgccctgtt cttatctctt
     1381 tgttccggga gttttgttca tgcgcaggag ggtaggtgga ggtttgtcag aacaagggac
     1441 gaggctccgg attgcagtga atgcgggtta gcagcggtca atggaaaact ctatttgatc
     1501 ggaggagacg gagaaggacc gaaagccgtt caatctttca acccggaaac gctcacctgg
     1561 acaaagcggg ctgttgcgcc gctgaccata catcattttc aggccgtcgc ctggcagaat
     1621 aaaatatatg tgctggatgc tttttcatcc ggtggatttc cggatcaggt tcccatgggc
     1681 catgtgtaca gttacgatcc ggaaaataat gcctgggaga aagggggaga aataccggct
     1741 gggagaaaaa gggccggagc ggcagcggtt gcctacaaaa acaaactgta tttaattgcc
     1801 ggcattcagc acgggcattc cagcggaacg accaatatgt ttgatgccta tgatcctcaa
     1861 actaaaacct ggaccgtctt acccgatgcg ccgcatatcc gcgatcattg ctccgccgcc
     1921 gtaataaacg ataaattata tgttgtcggc ggaaggaaca ccagttaccg ggatccggaa
     1981 aataaaatac cttttttcgc caaaactgtg ctggacgtgg acgtttacga tttcagcacc
     2041 ggcaaatggt cgactcatgc cgccaggctt ccgctgggta gcggaggagg ggccgtggtc
     2101 aatctgaata atgtgcttta ctatatgggc ggggagcgcg ccagttccac ggaacggaat
     2161 gctcccagga aaatacctac tacctggatc cgctgacggc cgatcactgg acggaaacgg
     2221 acagtctgcg ttttgcccgc aacggaatgg cagctgccgt ttgggaaaat agaatttatg
     2281 tggcgggtgg aagcggcggt ggcccgggag gcccgggtgg tccaagtggt cctccaccgg
     2341 caaacccctc cccgggaacg gcgcagcaaa gaccggttgc ggtggaggtc tttattttga
     2401 aaggaggaac tgaaaagaat taatattcga aggtttcata atggaaaaca tcctcctcaa
     2461 aaaataaaag gtatttttta tcctccggat aatatttcgc tttctgataa tccggcccgg
     2521 agaatttttt gatgctctcg tacgaatccc attcggtcac ggttaaaaaa tgacagacat
     2581 ctccatcgat cctcctcaac aattttgttg agatatttcc cggcaccgtt ttatactctt
     2641 tcagcccggt atcctcgaca tatttcaaat attcatccgc atgttccgct ttggtgatcc
     2701 cgtgccagac cctggttatt tttttcatgg gagtgctttg attgatgtgg caaaattaag
     2761 cgatgccggg ggaaatattc ccctggtcag acttttgtag cattgttcac aaaacgcaac
     2821 cagatgcatt cagcaggcca gaaaagaagg gggaatccga taccggcggc ggaaacggca
     2881 aaaaagataa aacgggttgt ccatccgcag ggtgtaaaga aaaaggtaca aaaggccccg
     2941 ccggtccggt aggacctcag cccgatcccg gtaacggctt ttccaaaaag gatctcaccc
     3001 atgctggcta ccctggcaga tcgggcattt gacgaaccgg gatggcttta cgaaatcaag
     3061 tgggacggat accgggccat tgcttattgc aatagatccg ccgtttccct gatgtcgcgc
     3121 aaccagaaat ccttcgacga taaattctat tccatccggg aggccataat ccaatggaat
     3181 attcatgcgg tggtggatgg cgtgataacg gtgctgaacg accagggtat ttccgatttc
     3241 ggcgggctgc agaactggcg aagcgaagcg gacggggaac tgatttacta tgtatttgac
     3301 ctgctctggc tgaacggcca taacctgatg gatcttccgc tgctccgcag gagggaattg
     3361 ctgaaagaac tcctgcctga aaacggtccc atccggttaa gcgagcattt tgagaccagc
     3421 gcaaaggaat ttctggaaag cgcacggaag ctgggactgg aagggatcat tgccaaaaag
     3481 gccgacagcc tttatcaacc gggagatcgt agccgcgact ggctgaaaat aaaactgagc
     3541 agacggcatg aagtggtgat cggcggctac acctgcaacg aaggttcttc caaggccttc
     3601 agctccctgc tcgtcggcgt tttcgaaaaa ggaaaattcc ggtatacggg taaggtcggc
     3661 accggttttt cctcccggca acaaaaggaa atgctctctt tgttcaaacc gctcattgtt
     3721 aagaaaacgg cgtttgaatc ggaaccggat atcaacaaac cgtcccggtt cagacccgat
     3781 ccgccgaaag caaaagcagt atggctcaaa cccaaactgg tttgcgaagt gcattatacg
     3841 gagatcaccg gagacggggt catgcggcat ccctcctttg aggccatgcg ggaggataag
     3901 gatgcgaagg atgtggggcc ggagaaagcg ttgc
//
LOCUS       DASYCU010000577         5231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_998949, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000577 DASYCU010000000
VERSION     DASYCU010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998949"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..3130
                     /locus_tag="VGM24_11650"
     CDS             86..3130
                     /locus_tag="VGM24_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4156080.1"
                     /translation="MLLACLCMNAFAQQTIRGTVISAKDGLPMPNVSVSIKGTTSGVV
                     TDSLGNFNLPASGTNIVLVFSYTGFASREVSAAGRNQVNVTLQPETNMLSDVVVIGYG
                     TARRSDLTGAVAAIKGEDLNDKPVPNVSQALQGKIAGVEVSINSNAPGDPAKVRVRGI
                     GSINSNIDPLYVVDGVIGVDGNSINPSDIASLEVLKDASSTAIFGARGANGVIMITTK
                     RGVKGKPILSYDGNVNMVDLYRHVKTLNADQFVQVYNLAYANGQKFDPAGGVWTPPAA
                     LNHANFPLLFDTNDKPLYNTNWEKEVYKPAYSQSHHLNLQGGSDKSLYSLSLGYLDQN
                     GLMIKSWFKRYSIRFTMDNDVYSWLRVGGAINLVKNTQRVVSDANGALNVPRMVTEEV
                     PIVPVKYADGTWAGNNDIAGLEGGPNPVHIANDRYTINNTMQMLGNVYLLFHITKDLD
                     LKSDFGYDLNSEKNNFYSSTDLAHLSQDQGGVANINAFNNYYWQTENYLTWNKQINDH
                     QKLTALLGASWLTSSYERVRSETQNFNDDILQWHYQQAGSVRSANESQDDSWSMNSYY
                     ARINYNINEKYLFTATGRYDGSSKFGKNNKYAFFPSVGAAWRISEEDFMKNNPSISNL
                     KLRGSYGVSGNQEIGQARSLAQIQPSTTVLGGARTSTLLPSYIGNPDLKWEKSLQADV
                     GLEMGLFNNRISLEVDYYNRVTKDLLLQAPIPWSAGMTNANVYRNVGSVRNAGIEAGL
                     TTVNIKTPDFTWSTTFLFASNKNKVLKLNDGNADIFPGPNFLGQNFVIRVGEPIGSFY
                     GMTRLGTYSDKEANEAAVHNLKPGDRKYIYNSDGSNYYSIIGQAYPKWTGNFSSSLTF
                     RNWDFSFDIRFVQGVNTAANFKHSSEDRQTIANSLATVLGAWTPDHQNTSISQVRNYK
                     FAQDSHFDSWWVEDGSFIRGQNFILGYSLPNSLTQKMKISKLHIYASVQNLFVITKYT
                     GYDPEVDTFNASYGSNGSFSQNLDFFAYPRPRTWNLGLSVQF"
     gene            3220..4725
                     /locus_tag="VGM24_11655"
     CDS             3220..4725
                     /locus_tag="VGM24_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4156081.1"
                     /translation="MKAIYYTVAFLLLLTCSCKKFLEEKPTYFLTPETPVSNLKDARA
                     FTDASYQNLQGFLAGQPGSYGGNVWNLMEFMTGKSNSDLGQTGFINFQNLSYNATSFY
                     VDTWWQQLYLGVGACNLAIEKVPTIVAAGLTDSTKTNMLAEAHTLRALYYFWLVRMYG
                     AVPKVTETPKDLNLKLSRSPAKDIYDSVIIPDLLLAEQSGLPWRDPNGRVSMGAVKSI
                     LADVYLTYAGAAINGGDSYYTESAKRSLDVIQNGGYNLFPEYTDMIRPSNKNIGEFIF
                     QVQYAAAVPSTNPLTALTIPNYSGISKYSDEYGSVYPTPQFVASFPAGDKRAQEKQFF
                     YTHYPSISTGTTVNFKGPYIYKWFDSVAVTNTVKSDLNYTIYRLADVMLMYAEASNRA
                     EGGPNVNAVTYVNDIRDRAKLPPIGALSQNAFEHEVWLQRYFELCFENKMWFDMLRTR
                     KVHNDVTGNWDDFVGHTTVFGATFAEKQLLFPVPKQETDVNPNLLPNNPGF"
     gene            complement(4772..>5231)
                     /locus_tag="VGM24_11660"
     CDS             complement(4772..>5231)
                     /locus_tag="VGM24_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156082.1"
                     /translation="GEYHYTRHQIWASTNPSKIVNLQLIYTWGPYFNGRLNSADWKFQ
                     FAPIPNISLTGEFNRNRFFGVGEDKTYSTVDLYIIQGRFALNPRLQLIGFYQRNSGNS
                     SRNYNIRFSWEYEPLSYVYLIYNHVEFSNPLGKNPGEDHVIAKISYLKQF"
BASE COUNT         1398 a         1246 c         1295 g         1292 t
ORIGIN      
        1 agcgttctgc tgtcgcgtta tttctgtgct aaaatcactt gatcaactta acaatcctgt
       61 tatgaataaa atttttattt gtaccatgct gttggcttgc ctatgcatga acgcctttgc
      121 acaacaaacg ataaggggaa cggtaatcag cgcaaaagat ggcttgccga tgcccaatgt
      181 gagtgtttcc ataaagggaa cgaccagtgg agtagtgacc gattcactgg gaaattttaa
      241 ccttccggcg tctggtacga atatcgtgct ggtattttcc tataccgggt ttgccagccg
      301 ggaagttagt gcggccggac ggaatcaggt caatgtaaca ctgcagccgg aaaccaatat
      361 gctgagtgat gtggtggtga tcggatatgg tacggcaaga agatcggacc tgacgggagc
      421 cgttgccgcc attaagggag aggacctgaa cgataagccc gtgcccaacg tatcgcaggc
      481 cctgcaggga aaaattgccg gggtggaagt gagtatcaac agtaatgctc cgggagatcc
      541 ggccaaagtg cgggtgcgcg gcatcggatc gatcaattcc aatatcgatc ccctctatgt
      601 ggtggacgga gtgatcggag tggacggaaa ttccatcaac ccgagtgata ttgcctcgct
      661 ggaagtattg aaggacgctt cttccaccgc cattttcggc gcgcggggtg ccaatggcgt
      721 catcatgatc acaaccaaaa gaggggtgaa gggaaagccg atcctttcct acgatgggaa
      781 tgtcaatatg gtcgatttat accgccatgt aaaaaccctg aatgccgatc agttcgtcca
      841 ggtatataac ctggcttatg ccaacgggca gaaatttgat ccggccggcg gggtctggac
      901 gccgccagcc gccctcaacc acgctaattt cccgctgtta tttgatacga acgataaacc
      961 cctgtacaat accaattggg agaaagaagt atataaaccg gcttactcac aaagccatca
     1021 cctgaacctc cagggcggta gcgacaagtc cttgtatagt ctttccctcg gatacctcga
     1081 tcagaacggg ctgatgatca aatcctggtt caagcggtat tccatccggt tcacgatgga
     1141 caatgacgtg tacagctggc ttcgcgtagg aggcgccatc aacctggtga aaaatacaca
     1201 gcgcgtggtg tccgatgcca atggggcatt gaacgtgccc aggatggtaa cggaagaagt
     1261 gcccatcgtt ccggtgaaat atgccgatgg aacctgggcg ggaaacaatg atattgccgg
     1321 actcgaaggc ggacccaatc ccgtgcatat tgcgaatgac cggtatacga tcaacaatac
     1381 catgcagatg ttggggaatg tctacctgtt gtttcatatt acaaaagacc ttgacctcaa
     1441 atcggacttt ggatatgatc tcaactccga gaagaacaat ttttattcat ccaccgacct
     1501 ggcccatctg tcccaggatc agggcggggt ggccaatatc aatgccttta acaactacta
     1561 ctggcaaacg gaaaactatc tcacctggaa taaacagatc aatgatcatc agaaactgac
     1621 tgccttattg ggcgcttcct ggctcacttc cagttacgaa agggtgcgtt ccgaaacaca
     1681 gaacttcaac gacgatatcc tgcaatggca ttaccagcag gcgggatccg tgagaagtgc
     1741 caatgaatcg caggacgatt cctggtcgat gaattcctac tatgcccgga tcaattacaa
     1801 tataaacgaa aaatatctct ttacggcgac cggccgctac gacggatcgt ccaagttcgg
     1861 caagaacaat aaatacgctt tcttcccgtc tgtcggcgcc gcctggcgca tttcggaaga
     1921 ggattttatg aagaacaatc cttccatcag caatttgaaa ctgcgcggaa gctacggggt
     1981 ttccgggaac caggaaatcg gtcaggcccg ttccctggcg cagatccagc cgagtactac
     2041 ggtcctcgga ggagccagga cctccacgct gcttccgagt tatatcggca atcccgatct
     2101 gaaatgggaa aaatcgctgc aggcagatgt cggcttggaa atgggattat tcaacaaccg
     2161 gataagcctg gaagtggatt attataaccg ggtcaccaag gaccttttat tgcaggcgcc
     2221 cattccctgg tccgccggta tgaccaatgc gaatgtatac cgcaatgtcg ggtccgtaag
     2281 aaatgccggg atcgaagccg ggctaaccac ggtaaatata aaaacaccgg attttacgtg
     2341 gtcaaccaca ttcctgtttg catccaataa gaataaggtg ctgaaattaa acgacgggaa
     2401 cgccgatatt tttccaggcc ccaattttct cggacagaat tttgttatcc gggtgggtga
     2461 gcccatcggc tctttctatg gaatgacccg tctgggaacc tacagcgaca aagaggcgaa
     2521 cgaggcggct gtccataatt taaaacccgg cgaccggaaa tatatctaca actccgatgg
     2581 cagtaattat tatagcatta tcggtcaggc ctatccgaaa tggaccggaa atttttccag
     2641 cagcctgacc ttccggaact gggatttttc ttttgatatc cgttttgtgc agggggtgaa
     2701 tacggcggca aattttaaac attcatccga agaccgccag accatcgcca acagcctcgc
     2761 caccgtgctg ggcgcgtgga caccggatca tcagaatact tcgatttcgc aggtgcgcaa
     2821 ctataaattt gcccaggatt cccattttga ttcctggtgg gtggaagatg gttcatttat
     2881 ccgggggcag aatttcatat tgggatacag cctgcctaat tccctgacac aaaaaatgaa
     2941 gatcagtaag ctgcatatat atgcgagcgt tcagaatctt tttgtgatta caaaatatac
     3001 cgggtatgat ccggaagtcg atacgttcaa tgcatcttac gggtctaacg gctccttctc
     3061 gcagaacctg gatttctttg cttatcctcg tccgcgcacc tggaacctgg gattgtcggt
     3121 acagttttaa aaccggcctt gaaagcggcc gttaacggtt cagcccgtct tcgctgaagc
     3181 tccggcgggc aagcgtaaaa acaaattaaa ttaattcaga tgaaagcaat ttattatacc
     3241 gtcgcctttt tactgttgct gacctgttcc tgtaaaaaat ttctggaaga aaagcctact
     3301 tattttctga ctccggaaac tcctgtcagc aacctgaaag atgcgcgggc attcacggat
     3361 gcctcttatc agaatttgca gggattcctt gcgggtcaac ccggctccta cgggggaaat
     3421 gtatggaacc ttatggaatt catgaccgga aaatcaaaca gtgacctggg acaaaccggg
     3481 tttataaatt tccagaatct ttcctataac gctacctcat tttatgtgga tacctggtgg
     3541 cagcaattgt acctgggcgt tggggcctgt aatctggcca tcgaaaaagt gccaaccatt
     3601 gttgcagccg gactgaccga ttccacaaaa accaatatgc tggcagaagc gcataccctg
     3661 cgggcattgt attatttttg gctggtaaga atgtatggag cggttccgaa agtgacggaa
     3721 acgcccaagg acctgaacct taaactgtcc cgttcaccgg cgaaggacat ctacgacagc
     3781 gtcatcattc ccgatctgct gctggctgaa cagtcaggct taccctggag ggatcccaac
     3841 ggccgggtgt ccatgggagc ggtaaaatca attctggccg atgtatatct gacctatgca
     3901 ggtgctgcca tcaatggagg cgattcctat tatacagaat ctgcgaaacg ctccctggat
     3961 gtaattcaga acggagggta taatctgttt ccggaataca cggatatgat caggccttca
     4021 aataaaaaca tcggtgagtt tatttttcag gtgcaatatg cggcggccgt tccctccacc
     4081 aatccactca cggcccttac gatcccgaac tattcgggca tatccaaata ctcggatgaa
     4141 tatggatccg tttatcccac tccgcaattt gtggcgtcgt ttcccgcggg ggacaaaagg
     4201 gcacaggaaa aacaattttt ctatacccat taccccagca tctccaccgg aaccaccgta
     4261 aattttaaag ggccttatat ttataaatgg tttgactccg tggcggtgac aaatacggtt
     4321 aaatcggatt taaattatac gatttaccgc ctggccgatg tgatgttaat gtatgcggaa
     4381 gcatccaacc gcgcagaagg cggaccaaat gtaaatgccg tcacctatgt gaatgatatc
     4441 cgcgaccgcg ctaaactgcc gccgattggc gcattgagcc agaatgcttt tgaacatgaa
     4501 gtctggttgc agcgttattt tgaattgtgc tttgaaaaca aaatgtggtt cgacatgcta
     4561 cggacgagaa aagtacataa tgatgttacc ggaaactggg atgattttgt gggacataca
     4621 actgttttcg gagccacgtt tgctgaaaag caattgctgt tccctgttcc gaaacaggaa
     4681 acagatgtta acccgaatct gctgcccaac aatccgggat tctagggaat tgggggttga
     4741 gaatgggtac aacattctca actctcttct cttaaaactg tttgaggtag ctgattttcg
     4801 cgatcacatg atcctcgccc ggattcttcc ccagcggatt gctgaattca acatggttat
     4861 aaatcaggta tacgtaggag aggggttcat attcccagga aaaccggata ttatagttcc
     4921 gggaactgtt tccggaattt ctttggtaaa acccaatcag ctgcagcctt ggattcaatg
     4981 caaagcgccc ctggataata taaagatcca ccgttgaata ggttttgtct tcaccaactc
     5041 cgaaaaaacg gttgcggtta aactctcccg ttagtgagat gttggggatc ggggcaaact
     5101 ggaatttcca gtcagcagaa ttcaggcgac cgttaaaata gggaccccag gtatagatga
     5161 gttgcaggtt tacgatttta gacggattgg tgctggccca gatctgatgg cgtgtataat
     5221 ggtattcccc g
//
LOCUS       DASYCU010000578         3979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_707556, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000578 DASYCU010000000
VERSION     DASYCU010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707556"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGM24_11665"
     CDS             complement(<1..351)
                     /locus_tag="VGM24_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156083.1"
                     /translation="MSINSNIQSDAPGSASAPGSGSSARLTPETPIPSTPAVLASLLR
                     ADFTTQWRNRKSFMLILIVPVIIMISWKGLIDRLGSAFVLGNCITIGLIAIGLMGYSN
                     SVARDRDKGIFQRLR"
     gene            complement(374..1153)
                     /locus_tag="VGM24_11670"
     CDS             complement(374..1153)
                     /locus_tag="VGM24_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4156084.1"
                     /translation="MNLPLEAVLEVRDLNVKYGSFQAVKNVSFTVQPGEIFGLLGPNG
                     AGKTSSLSAVEGLLIPESGVIRVAGYDIKTHPLHARANMGVQLQATSFQPELNVSEIL
                     KLYAGIYGLELTRDKIESILDKINLRDAASKRFGQLSGGQQQRVSLVIALIHEPRLVL
                     LDEPTTGLDPQSRRQLWERMEYLRNQGHGILLTTHSMEEAEAVCDRIAIIDHGQVIIT
                     DTPQALIDKHRSNPEVIRASRKGRITLEDVFIGLTGSQVRA"
     gene            complement(1337..2629)
                     /locus_tag="VGM24_11675"
     CDS             complement(1337..2629)
                     /locus_tag="VGM24_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY4156085.1"
                     /translation="MNMISGMPFFRPVLLFPVLFLSLFRLNAQAGPKDKSGLPVRNAG
                     LPNGIWQASIIRNDGRQIRFNFETKDSAGKKILYVMNGKERLLVDRIRQRGDSAYIEM
                     PFFDSRFALRLSGTDRLDGIWIKNYGDRRVTIPFRAIRNRPGRFPVSRPPSFDISGRW
                     SAHFEKEEPAAVGEFKQTGARVTGTFLTTTGDYRFLEGVVDGDSLRLSTFDGGHAYYF
                     TAKIDGGDKLSGGIFYAGASSRESWEAERNEQAALPDEYSLTRLKNPHRATLDFKFKS
                     IEGPVISIKDKAFKNKVVIVQIMGSWCPNCMDETRFLSDYYRENHRRGVEIIALAYER
                     TTHFAESKRSLQSFKNRFHVSYPILVTGVAVGDSLRTEKTLPQIREIIGFPTTLFIDK
                     KGLVRKISTGFNGPGTGDHYEIFKKEFNELVDGLLKEE"
     gene            complement(2651..3898)
                     /locus_tag="VGM24_11680"
     CDS             complement(2651..3898)
                     /locus_tag="VGM24_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY4156086.1"
                     /translation="MTGLNRFKKYPLAILFIFLFIYLGNMFCEGSGTLKSLPEQAEDQ
                     TRYAGSASCKNCHKDIYDAQLLTAHYMDSRPALEKFIKGSFKKPKNRFRLNKFTEVVM
                     NKTDSGFFQSAFINDILFRQEAFGMVIGSGRKGQSYLYWDGGRMFQLPVSYFTPLNSW
                     CNSPGYNNLEAYFNKAISGRCIECHSTYAKTTIDPDGVTRFDSSSIIYGIDCERCHGP
                     SAAHVEFHLKNPNEKQARFVIDPKSLSRERRMDACALCHSGFRKEKLPPFSFLTGDQL
                     DDFSTPDYKEENSSTLDVHGNQFGLLTSSKCFKSSRMDCSSCHDTHHNEVNNTLLFSS
                     RCMNCHKEHSPDACTFNPPKGLILSNNCVDCHMPLLASRKIFLQMDDPQKSTADLVRT
                     HRIAIYPDITKKFLKSGAARGIN"
BASE COUNT          974 a          977 c          991 g         1037 t
ORIGIN      
        1 ccgaagccgc tggaaaatac ccttatcacg gtcacgggca accgaattcg aatatcccat
       61 cagaccaatg gcaatgagtc cgatcgtgat gcagttgccc agaacaaaag cgctgcccag
      121 ccggtcgatc agacctttcc aggaaatcat gataatgacc ggtacaatca ggatgagcat
      181 aaaggatttc cggttgcgcc actgggtggt aaagtcagcc cgtagtaagg aagccagcac
      241 ggccggagtg gacggtatag gggtctccgg agtgagccgg gcggaagatc cgctgcccgg
      301 cgctgatgct gaacctggcg cgtctgattg aatgtttgag tttattgaca tgctaattta
      361 ttttgaaatg cggttaagcc ctgacctggc tgccggtgag accgatgaat acgtcttcca
      421 gtgtaatcct gcctttgcga gatgcccgga tgacttccgg attgctccgg tgtttgtcaa
      481 tcagcgcctg gggcgtatcg gtgatgatca cctgcccgtg atcgatgatc gcgatccggt
      541 cgcagacggc ttctgcctct tccatggaat gggtggtcag caatatacca tggccctggt
      601 ttctcaggta ttccattctt tcccaaagct gccgcctgga ttgcgggtcg aggcccgtag
      661 tcggctcatc cagcagcacc agccggggtt cgtgaatcag cgcgatcacg agcgaaaccc
      721 gttgctgctg accaccggaa agttgcccga atcgtttgga agctgcgtcg cggagattaa
      781 tcttatccag gatggattcg attttatcgc gcgtcagttc taggccataa attccggcat
      841 agagtttcag tatttcagaa acgttaagtt ccggctgaaa actggttgcc tgaagctgta
      901 cgcccatatt ggcacgggca tgcagcggat gggtctttat gtcatatccg gcaacccgga
      961 taactccgga ttcgggaatg agcaagccct cgactgcgct cagggaggac gttttgccgg
     1021 cgccgttcgg gcccagcagt ccgaaaattt caccgggttg tacagtgaat gaaacgtttt
     1081 ttactgcctg aaaggatccg tatttcacgt tcaggtcgcg gacttccaat acagcttcta
     1141 aagggagatt cataatgatt aactttttat gggcaggcct tgtttttgtt tgaacccgca
     1201 atagctactt ctttatccgg agaaaatatc gttccctgat caggccgcga agatatttcc
     1261 gaatctcaat gaagaaaagc agtaagcggt gaatggctgg cgggaagcgg tgaaaacgcc
     1321 gggatttacg gtaggcttat tcctctttca gcaaaccatc caccagttca ttaaattctt
     1381 ttttaaatat ttcataatga tcacccgtgc cgggaccgtt aaaaccggta gaaatttttc
     1441 ttaccagtcc ttttttatct atgaacaggg ttgttggaaa tccgatgatc tccctgatct
     1501 gcgggagggt tttttccgtt cttaacgaat cgccgacggc cacgccagta accagaatcg
     1561 gataacttac gtggaaccgg tttttaaatg attgcaggga tcttttagac tcggcaaaat
     1621 gggtggttct ttcataagcc agggcgatta tttccacgcc ccgccggtga ttctctctat
     1681 aataatcgct caggaagcgg gtttcgtcca tgcagttcgg gcaccaggag cccatgatct
     1741 ggacaatcac caccttgttc ttaaacgctt tatcctttat ggagatcacc ggaccttcaa
     1801 tacttttaaa tttgaaatcc agtgttgccc ggtgagggtt tttaagacgg gtcaggctat
     1861 actcatccgg taaggccgcc tgctcatttc tttctgcctc ccaggattcc ctactgctgg
     1921 ccccggcata aaaaatgcct cccgatagtt tatccccccc gtcaatcttt gcggtaaaat
     1981 agtaggcatg gcctccatca aaagtggaca gccggaggga atccccgtcc actacaccct
     2041 ccagaaaccg gtagtctccc gtggtggtca gaaaagttcc ggttacccgg gcgcccgtct
     2101 gtttaaattc tcctaccgca gccggttctt ccttttcaaa atgcgcggac cacctgccgc
     2161 taatatcaaa ggagggcgga cgggaaacgg gaaaccgccc cggccgattc cggattgcgc
     2221 ggaagggaat ggttacccgg cggtcgccat aattttttat ccagattcca tccagccggt
     2281 ccgttccgga aaggcgcaac gcaaaccggg aatcaaaaaa cggcatttca atatatgccg
     2341 aatccccccg ttgccggatg cgatccacca ggagccgctc ttttccattc atcacataga
     2401 gtattttttt accggcactg tcctttgttt caaaattaaa acggatctgc cggccgtcgt
     2461 tgcgaataat gctcgcctgc cagattccgt ttggcagccc tgcattccgc accggcagac
     2521 cgcttttgtc ctttggcccg gcctgggcat taaggcggaa aagagaaaga aaaagaaccg
     2581 gaaaaagcaa tacgggccgg aaaaaaggca taccagagat catgttcatg taaaaattgg
     2641 aatcacaaat ttaattaatc ccccttgctg ccccgctctt taaaaatttt tttgtgatat
     2701 ccggataaat cgcgatccgg tgggtcctaa ccaggtctgc cgttgacttt tgaggatcat
     2761 ccatctgcaa aaagatcttc cgggaagcca gcaaaggcat gtggcagtcg acgcaattat
     2821 tgctcaggat caatcctttt ggcgggttga acgtgcaggc gtccggacta tgctctttgt
     2881 ggcaattcat gcatctggag gaaaataaaa gggtattgtt cacctcattg tgatgggtat
     2941 catgacagga agaacaatcc atccgggagg atttgaaaca cttgctcgat gtcaggaggc
     3001 cgaattgatt accatgtaca tccagagttg aactgttctc ttctttataa tcaggagtcg
     3061 aaaaatcatc cagctgatca cccgtaagga atgaaaaagg aggcagtttt tcctttctga
     3121 atccggaatg acagagggcg caggcatcca ttctccgctc ccgggaaagt gatttcggat
     3181 cgattacaaa ccgggcctgt ttttcatttg ggtttttgag atggaattcg acatgcgctg
     3241 cggatggtcc gtgacagcgt tcgcaatcta ttccatagat gatcgagctg ctgtcgaagc
     3301 gggtcacccc atccggatcg atggttgttt ttgcataggt ggaatgacat tcgatgcagc
     3361 ggccgctgat ggccttgttg aaataggctt ccagattatt atagcccggg ctgttgcacc
     3421 agctgttcaa gggagtgaaa taggaaaccg gaagctgaaa cattctgccg ccatcccaat
     3481 agaggtagga ttgacctttt cttcccgaac cgatcaccat cccgaaagct tcctgcctga
     3541 acaggatatc gtttataaaa gcgctttgaa agaaaccact gtccgtttta ttcataacca
     3601 cttccgtgaa tttgttaagt ctgaaacggt tcttcggttt tttgaaactc cccttaatga
     3661 atttctccag ggctggcctg gaatccatat agtgtgccgt gaggagttgt gcatcataga
     3721 tgtccttatg gcagtttttg caggacgcgg agccggcata gcgggtttga tcttcggcct
     3781 gttccggcag gctttttaat gttccggacc cctcacagaa catatttccg aggtatataa
     3841 aaaggaagat gaaaaggatc gcgagcggat actttttaaa tctgtttaag cctgtcattc
     3901 ccacagcggt taatcccgaa caatttaaat catttaaaga agacttcaaa ttgagcttaa
     3961 attagcagat cattcacgg
//
LOCUS       DASYCU010000579         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_414789, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000579 DASYCU010000000
VERSION     DASYCU010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414789"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VGM24_11685"
     CDS             <1..1207
                     /locus_tag="VGM24_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY4156087.1"
                     /translation="LLIALAPHAVPDLFDRAIQEKIKASGDFPEIGGVRGKNFRGFLP
                     TGQTAVFLLAGEDRDRQMEVEQMFWSDRELAIRKILWLEDLPPGEPMLSGRVIMASDY
                     INLFLYGMSAPPQFSTTFPARRISTHLRTEDIMMTGEIRRHYKSLQEWLQFNTSLMEE
                     WDMKKRLKQGYRVLFYGPPGTGKTLMANVLGNETAREVYKIDLSMIVSKYIGETEKNL
                     ELLFARAEDKNWILFFDEADAIFGKRTSVKDAHDKYANQEVSYLLQRIEEFNGLIILA
                     TNMKNNIDEAFLRRFNDLVKFTLPNEDERREIWEKSFPGRADYGDIANQVKRHEISGG
                     NIINAIHFAGIQAVRKKTAGVGRKELMALNGNGSHAESGPDQLVFYLEDVLEGIRREM
                     IKEGKPFSV"
     gene            complement(1285..3675)
                     /locus_tag="VGM24_11690"
     CDS             complement(1285..3675)
                     /locus_tag="VGM24_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4156088.1"
                     /translation="MLKNYFKTAFRSLSRNRVYSLINTAGLAVGIAVCLVIFIFIRYE
                     QSFDNFHPNGERIYRLLTKGDKQDSTSAVPFPLPTAIENDYPDWKVSGIFSQNNLLVN
                     IPGKDGQAEKKFKEKSGGFLVEPSFFRIFHFPWLAGDPANALLAQDDVVLTRAVAEKY
                     FGDWKAAMGKTMKINGHSMVTVTGILADPPSNTDLQLKVVFPYSRANFSKNKDWWTIN
                     SNHGCYLLLPPNTSPARVNRQLAALSKKYRTPDNKSTQVIQALREVHFDTKAGNFGGR
                     TIAPDRIRNLWLIAAFILVIACVNFINLSTAQAVNRAREVGVRKVLGGNRLQLTKQFL
                     LETGMLVLTGVVLAVLITALLLQPVGNILDIPLSYHFFLTNNVPLFLVAVTGAVTLLA
                     GFYPALVLSAFNPIIALKSKLAARNAKGVSLRRGLVVFQFIIAQGLIIGTLLISKQLS
                     YFENVPMGFDKKAIVSVPFPADSLGISKIDYLRNRLLAVKGIERVSFSGAPPADDDNW
                     WTPFNFNHAEKETNFASIQKWVDPNFLATYSLQLVAGRNFSGNDSVKEFIVNEKLVEK
                     LGFSDPHAVLNKEINLWNGFAIGRIVGVIKNYYTASMRDSLAPVFMMNLKRGCSTAGI
                     KMNATDLNSLLTSVKGIWASTFPDYVFEYQFLDEKIASFYKEETQLSNFYKLFAGIAI
                     FLSCLGLYGLASFMAVQRIKEVGIRKVLGATIGSIVYLFSREFVLLIGIAFVIAAPLA
                     WYFVHQWVQNYVYRISISGWVFILGGMGALLVALATVSFQAVKAGKANPASSLRSE"
     gene            3963..>4644
                     /locus_tag="VGM24_11695"
     CDS             3963..>4644
                     /locus_tag="VGM24_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156089.1"
                     /translation="MNPRVITLAVLMLSGALHAQRWTQLNPPASLFNNSIFSVVADHS
                     GKLYAAGGGTDSAGENAVAVLINGAWSELGVGGHTLHAKGNIYSLALDPSGNLYSAGA
                     FTDSSGNYYVAKWNGSDWSETGPASSAQNFTGQIFSLAADRLGNIYAGGEMIDSLGNY
                     YVSKFDGTQWQPLGSGLQSLRANGIIYAVLADENGNIYAAGQFTNADGNWYVAKWNGS
                     SWSELGTGA"
BASE COUNT         1182 a         1164 c         1209 g         1089 t
ORIGIN      
        1 gctgctgatc gcccttgcgc ctcacgcggt accggatttg ttcgaccggg cgatccagga
       61 aaagatcaag gcatccggcg attttcccga aatcggcggt gtgaggggga agaatttccg
      121 gggatttctg cctacgggcc agaccgctgt cttcctgttg gccggcgagg accgcgacag
      181 gcaaatggaa gtagagcaga tgttctggtc cgaccgcgaa ctggctatcc gcaaaatatt
      241 gtggctggaa gaccttccac cgggagaacc catgctcagc ggccgggtca tcatggcatc
      301 cgactatatc aatctttttt tatacggcat gtcggccccg ccgcaattca gcaccacctt
      361 cccggcgcga cggatcagta cgcatttacg cacggaagac atcatgatga ccggtgagat
      421 caggcgtcat tacaaaagct tgcaggaatg gctgcaattc aataccagcc tgatggaaga
      481 atgggacatg aaaaaaagac tgaagcaggg ataccgcgtg ttgttctacg gcccgccggg
      541 aacgggcaag accctgatgg caaatgtgct gggcaacgaa acggcaagag aggtctataa
      601 gatcgacctc tcgatgatcg tttccaaata tatcggcgaa acagaaaaaa acctggagct
      661 tctctttgcg cgcgcggagg ataagaactg gatcctgttt ttcgatgagg ccgacgccat
      721 cttcggcaag cgcaccagcg tgaaagatgc gcacgataaa tatgcgaacc aggaagtttc
      781 ctatctgttg caacgtattg aagaattcaa cgggctgatc atcctggcta cgaatatgaa
      841 gaacaatatt gatgaagcct ttttacggcg cttcaacgac ctggtgaaat tcacgctgcc
      901 aaacgaggac gagcgcaggg agatctggga gaaatcattc cccggccgcg cggactatgg
      961 cgatatcgcc aaccaggtaa agcggcatga gatctccgga gggaatatta ttaatgccat
     1021 ccatttcgcg ggtatacagg cggtcaggaa aaagactgcc ggggtaggac gcaaagaatt
     1081 gatggcgttg aatggtaacg gcagtcacgc tgagtccggg ccggatcagc tggtctttta
     1141 tctggaagat gtgctggaag gaataagaag ggaaatgatc aaggagggga agccgtttag
     1201 tgtttgagca agtctaccct cgttatcctt gacccccctt gaatctgcca gtcttcgatg
     1261 agttgaaaat gcaactaacc gtgctcattc cgaacgcagg ctgctggcgg gattagcctt
     1321 ccccgcctta accgcctgaa agctcaccgt tgccaatgca accagcaagg cgcccattcc
     1381 acctaatata aacacccacc cgctgatgga tatccggtaa acataattct gcacccactg
     1441 atgtacgaag taccaggcca gcggagccgc gatcacgaag gcaatgccga tcagcagtac
     1501 aaattcgcgg gagaaaagat aaacgatact cccgatggtt gcgccgagca ctttccggat
     1561 tcccacttcc ttgatgcgct gcaccgccat gaaagaagcc agaccataaa ggcccaggca
     1621 actcaggaag atcgcgatgc cggcaaataa tttatagaaa ttcgacagct gtgtctcctc
     1681 cttgtaaaaa gatgcaattt tttcatccag gaactggtac tcgaaaacat aatccggaaa
     1741 agtgctggcc catattcctt taacggatgt cagcaaacta ttcaggtcgg tggcattcat
     1801 ttttattcct gcggtactac aacctctctt caggttcatc ataaagaccg gagccagcga
     1861 atcccgcatg gaggcagtat aataattttt tatcactcca acgatgcggc caatggcaaa
     1921 gccgttccag agattgatct ctttgttcag cacagcatgc gggtcggaga acccgagttt
     1981 ttccaccagc ttctcgttca cgataaattc cttgaccgaa tcattcccgg agaaattcct
     2041 tccggctacc agttgcagcg agtaggtggc caggaaattc gggtccaccc atttctggat
     2101 cgaagcgaaa ttcgtttcct tctccgcatg attaaaatta aaaggtgtcc accagttatc
     2161 atcatccgcc ggcggcgcgc cgctgaagct tacccgttca attcctttga ccgccagcag
     2221 gcggttccgc aaatagtcaa tcttgctgat ccccagactg tctgcgggaa agggcaccga
     2281 cacgatggcc tttttgtcga atcccatcgg cacattttca aaatagctga gctgcttgct
     2341 gatcaggagc gttccgatga tcaggccctg cgcaatgata aactggaaca ccaccagccc
     2401 tcttctcagc gaaacccctt tcgcgttcct tgcggctagt ttgcttttca gcgctatgat
     2461 cggattaaaa gcggaaagta cgagcgcagg gtaaaagcct gccagcagcg tcactgcgcc
     2521 ggtaacggca acgaggaata agggcacgtt gttagtcagg aaaaaatgat aggagagcgg
     2581 aatatccaaa atgtttccca caggctgcag cagaagcgcg gtgatcagaa cggcgagtac
     2641 gacgccagtc agcaccagca tcccggtttc caggagaaat tgtttcgtga gctgcaaccg
     2701 gtttccgccg agtacttttc ttacgccgac ttcccgtgcg cggttcacgg cctgtgcggt
     2761 ggacaggtta ataaaattaa cgcaggcaat tacaagaata aaagcggcga tgagccagag
     2821 gttgcggatg cggtcgggcg caatggtcct gccgccgaag ttaccagcct tggtatcgaa
     2881 atgcacttcc cgcagagctt gtattacctg ggtacttttg ttatccggtg tccggtattt
     2941 tttagaaagt gcggcgagtt gcctgttcac tctggccgga ctggtattcg gtggcaacag
     3001 cagataacat ccatgattgc tattgatggt ccaccagtct ttattttttg aaaaatttgc
     3061 acgactgtat gggaaaacga ccttcagctg aagatccgta ttcgaaggcg gatctgccag
     3121 tatgcccgtg acggtgacca tggagtggcc atttattttc attgtctttc ccatagccgc
     3181 cttccagtcc ccgaaatatt tttcggccac tgccctggtc agcacaacat cgtcctgcgc
     3241 cagcaatgcg ttggccggat cgcccgccag ccaggggaaa tgaaatatgc ggaaaaaaga
     3301 aggctctacg agaaagccac cggatttctc cttaaatttt ttctccgcct gcccgtcctt
     3361 gcccggaata ttgaccagca gattattctg ggaaaatata ccggatacct tccagtccgg
     3421 atagtcattc tctatcgccg ttggcagggg aaagggaacg gctgaagtgg aatcctgctt
     3481 atctcctttc gtcagcagcc ggtagatcct ttccccgttg ggatgaaaat tatcaaaact
     3541 ctgttcgtac cggatgaaaa taaagatcac caggcaaacc gcgatgccga ccgccagccc
     3601 ggcagtattg atcagcgaat agaccctgtt ccgggacaag ctccggaaag ctgttttgaa
     3661 ataattcttc agcatacaac gccggtttat gttttgggaa gaaaaaatga caataggtga
     3721 cagcgaagaa aatgcctgaa ttgtaaagaa ctattaacca gtaatttgaa ttttcagggc
     3781 ctgcctccgg gtgtacgctt ttgatacgct ggctgggcgg ttttgcagaa ttccaatgca
     3841 tcccggcgtc tactggttat aagcttacgc taaaaaggaa gaaccgggag atttgattga
     3901 atttgcgcag aaggttagga tttaaggggg caaaagagta tttttcgccc tcaaacgatc
     3961 cgatgaaccc ccgggttata acgctggctg ttttgatgct ttccggcgca ttgcatgcgc
     4021 aacggtggac acaattaaat ccgccggcca gcctgttcaa taacagcatt ttttccgtcg
     4081 ttgcagacca ttcgggtaag ctgtatgctg ccggcggggg caccgactcg gccggtgaaa
     4141 atgccgttgc cgttttaatc aacggtgcct ggagtgaact gggcgtaggt ggccatacgc
     4201 ttcatgcgaa gggaaatatt tattcgcttg cgctcgatcc ctccggcaat ttatattccg
     4261 ccggcgcatt taccgattct tccgggaatt actatgtagc gaaatggaat ggaagcgatt
     4321 ggtcggaaac aggacctgcc agttccgcgc aaaatttcac cgggcagatc ttttctcttg
     4381 ctgctgaccg gcttggaaat atctatgccg gcggggagat gatcgactcg cttggaaatt
     4441 attatgtctc caaatttgac ggcacgcaat ggcagccgct cggatccggg ttacagtctt
     4501 tgcgtgcaaa cggtatcatt tatgctgttc ttgcggatga aaacggaaat atttatgccg
     4561 caggccagtt taccaatgcc gacgggaatt ggtatgtcgc aaaatggaat ggcagcagct
     4621 ggtcggaact gggaaccggt gcag
//
LOCUS       DASYCU010000580         7556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1866902, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000580 DASYCU010000000
VERSION     DASYCU010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866902"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VGM24_11700"
     CDS             complement(<1..87)
                     /locus_tag="VGM24_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HEY4156090.1"
                     /translation="MFAVVQISGSQFRVEKDQTLYVPRIDGKT"
     gene            231..1727
                     /locus_tag="VGM24_11705"
     CDS             231..1727
                     /locus_tag="VGM24_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HEY4156091.1"
                     /translation="MKIKSFFAKPFASYIYRSTRKEMGSALEDQENILKSLLKNGSKT
                     VFGREHHLETVKTYEEFKAAVPIRDYEEFKPYIDQIRQGKHNVLWKGQPIYFAKTSGT
                     TSGIKYIPISRDSISNHINTARNALLCYMAETGNSAFANGKMIFLSGSPELERIAGIP
                     TGRLSGIVNHHIPHYLRTNQLPSYETNCIEEWEAKLDKIVEETIRQNMTLISGIPPWM
                     QMYFDRLMQVSGKQIKDIFPNFSVLVHGGVNFEPYKDRLLDSIGKPVDTIETYPASEG
                     FFAFQDSRQEEGLLLNSNSGIFFEFIPAAEIYNESPTRISLKDVKAGVNYALVVSNNA
                     GLWGYNIGDTVRFVSTSPYRIVVTGRIKHFISAFGEHVIGEEVEYSLMKAAKEEGVQI
                     NEFTVAPFIRRQGEGKSFHEWFVEFEHRPADIHAFARKVDSNLREKNIYYDDLISGNI
                     LQPLQIHPVKKNGFIDYMKSIGKLGGQNKVPRLSNDRSFAEELEKYSD"
     gene            1784..2953
                     /locus_tag="VGM24_11710"
     CDS             1784..2953
                     /locus_tag="VGM24_11710"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY4156092.1"
                     /translation="MMNNQQKKRVALFGSTGSVGTQALEVIEANPELFSAEVLTAHQN
                     AELLIKQANKFLPDIVVIGDESKYQMVRQALEAHPIKVFCGEKALMEVASMDSYDVML
                     AAIVGFAGLMPTLKAIENGKAIALANKETLVVAGDIVMRKAVENRAPIIPVDSEHSAI
                     FQCLLGETRNRIEKIVLTASGGPFLGKKPNFLVNVKRDHALQHPNWAMGAKISIDSAT
                     LMNKGLEMIEAKWLFNLQPQQIQVVIHPQSIIHSMVQFEDGSIKAQMGLPDMKLPIQY
                     ALGFPLRLPNKMPRYSFNKPDKLSFEDPDIRTFRNLQLATDALKKGGNAGCVLNAANE
                     IAVYAFLKNRIGFLDMTDLIEKTIQKIPFLEQPTLEDYFDSDGEARNFAASLIKL"
     gene            2969..4390
                     /gene="rseP"
                     /locus_tag="VGM24_11715"
     CDS             2969..4390
                     /gene="rseP"
                     /locus_tag="VGM24_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY4156093.1"
                     /translation="MQGRALRLIFVKMNNTPILLAIDWANAGSKAAQLILSLSLIVIV
                     HEFGHYLPAKIFRCRVEKFFLFFDPWFALVKKKVGETVYGIGWLPLGGYVKIAGMVDE
                     SMDKEQMKQPPQPWEFRSKPAWQRLIIMLGGVTMNVLMAFVIYAMILFTWGEKKIPMS
                     GITYGVSVTDSLMYEMGFRNGDKILSVNGEPVTYFDDLTKTLLLGENVELERDGKKQT
                     INLPVNLIGKLVEKKRTGQPFFIPRIPVIADQVPDTSNAYKAGLRRLDQITAVEGIAT
                     PFYEEYKKAVDAHKGQSIRLTIDRNSKTDTLHADVNADGTLGFYRLGDLETLDSLGLI
                     RIERKEYSFFASFPAGIHLAGETLKSYIDQFKKVLSPKTGAYKGVGGFKAIGSIFPSM
                     WDWQSFWTITAFLSVILAFMNILPIPALDGGHVLFTLLEMITGRKPSQKVLEYAQVIG
                     MVLLLGLMIYANGNDWFGWGRGK"
     gene            4537..5601
                     /locus_tag="VGM24_11720"
     CDS             4537..5601
                     /locus_tag="VGM24_11720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY4156094.1"
                     /translation="MKQESTTGFYYIGTQSAHMAKLVSIYKRGFASDNMQQAITELTR
                     EKHSPPEVICCEARFGLAAITRFAGFLAKHPTLSRIPFILDADRMTTGDHEEYIKNKL
                     ADEILFLRDYDENLLSRKIRFLQNFKTKTTELDKLEKAEISAQVPASLYNFLKRSFEI
                     LVALFLLFTLAPLFLLIAIALKLESGGPVFYVSKRAGKGYRIFNFYKFRTMYDGSDQH
                     MVELAHLNQYRGSREPRFFKVKNDPRITRIGQFLRNTSLDELPQLINVLIGDMSLVGN
                     RPLPLYEAETLTTDALAKRFLAPAGITGLWQIKKRGKQKMTEEERISLDIEYADNSNF
                     LYDLWIIANTAQAMIQRENV"
     gene            5634..7556
                     /locus_tag="VGM24_11725"
     CDS             5634..7556
                     /locus_tag="VGM24_11725"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY4156095.1"
                     /translation="MIARTGHKYSFLYAIREFLFPCIRLRVLLVCLFLTGSHWLPAQS
                     AYVDSMIRWAESHPRTDSQKIRTLHRISYMLSETDVNRSFAYYEQVSSLSDSLHFIFG
                     KALASINLGILLSSAGNFESSTKAFFNAIDLADSCAATRVKAVALNNIGENFFSLREY
                     NKCRSYARQAIDINTRVRAWRGVAINYELMQRCDFEQGRYSDARENLDKGMPYARLAN
                     ENYIYSPFYLGYGKLKAVSNQKDSAGFFFDKAITSARQDADLRNEYQVYLAKAKYQKF
                     LPVRNKIELLSKALGLANQTHFEEGRAKAAQELSSMYDNLNREDSSLFYYRMYRSATD
                     SLFSEKNRRNMIVNESEWVVKRKELENSNLKELAAVQKKQIAFKNILLFFIGMGFVLT
                     LLVAFLIYKSIQSKKLKEELAYKQKIAETEMQALRAQMNPHFFFNSLNSIENFIMQNE
                     KKLASDYLNKFARFIRSILDSSYSELIEIKKDVESLQLYIDLERLRYNDKFRFCCRVD
                     PLLIDGEYHVPSLLVQPFLENAIIHGIGPSDRNDLKLTLLIRLKDSGIQYVIEDNGIG
                     REQSRAYKDLNKPFHKSVGMKITQDRINIFNEDAPAADSVKIIDLYDDQQRPSGTRIE
                     FTIKLVSYASPKSRIG"
BASE COUNT         2039 a         1816 c         1878 g         1823 t
ORIGIN      
        1 ggtttttcca tcaatccggg gaacgtagag cgtctggtcc ttttcgaccc tgaactggct
       61 gcctgagatt tgtacaactg cgaacataac gatgcaatat tagggctgca aaggtaaggg
      121 gattcggggt aatttcgaag gattcgggta aaaaacttga atggccgggc ccctgggcgg
      181 ggtattggct tttattgtag gtttgcgttc aactttgcct gcctgaaatt atgaagataa
      241 agtcgttttt tgccaagccc tttgcttctt atatatatag gagtacccgg aaggagatgg
      301 gatcagccct ggaggaccag gagaatatcc tcaaatccct cctgaaaaac ggttcaaaga
      361 ccgtattcgg gcgggaacat cacctggaga ccgtgaagac ctacgaggag ttcaaggcag
      421 ccgtccccat ccgcgattat gaggaattca agccttatat tgaccagatc cggcagggaa
      481 agcacaatgt gctctggaaa ggccagccga tctattttgc caagacctcc ggaaccacca
      541 gcggcatcaa atacattccg atttccaggg actctatttc caaccacatc aatacggcgc
      601 gtaatgccct gctttgctat atggcggaga cgggcaattc ggcctttgcc aacggcaaaa
      661 tgatctttct ttccggttcc cccgaactgg agcgtattgc cgggatcccc acgggcaggc
      721 tcagcggcat cgtgaaccac catattccgc attacctgcg caccaaccag ctgcccagtt
      781 acgaaaccaa ctgtattgaa gaatgggaag ccaagctcga caaaattgta gaggaaacca
      841 tccggcagaa tatgaccctc atcagcggga tacctccctg gatgcagatg tattttgacc
      901 gcctgatgca ggtatccggc aaacagatca aggatatttt cccgaatttt tccgtcctgg
      961 tccatggcgg cgtaaatttt gagccgtata aagaccgctt gctggacagt atcggcaaac
     1021 cggtggacac gatagaaacc tatcccgcat cggaaggatt ttttgcgttt caggacagcc
     1081 gtcaggaaga aggcctgtta ttaaacagca atagcggcat ctttttcgaa ttcattccgg
     1141 ccgcggaaat ttataacgag agcccgaccc gcatttcttt aaaggacgtg aaggccggcg
     1201 tgaactatgc gctggtggta agcaataatg caggactctg gggatataat atcggagaca
     1261 cggtccggtt tgtttccacc agcccgtacc ggatagtcgt tacgggccgg atcaaacatt
     1321 ttatatctgc tttcggcgaa cacgtgatcg gcgaggaagt ggaatacagt ttaatgaaag
     1381 cggccaaaga ggaaggcgtg cagatcaatg aattcacggt ggcccccttt atccggcggc
     1441 agggggaagg gaaatccttt catgaatggt ttgtggaatt tgaacaccgg ccggccgata
     1501 ttcatgcctt tgcccgtaag gtggacagta acctgcgcga gaaaaatatt tattacgacg
     1561 acctcatctc cgggaatatt ctgcaacccc tgcagattca ccccgtcaag aaaaatgggt
     1621 tcattgacta tatgaaatcc attggaaaac tcggcggaca aaataaggtg ccacgcctca
     1681 gcaacgaccg gagttttgcg gaagaacttg aaaaatacag tgactgatct ttcgcggcat
     1741 acccgtggaa attactattt ttaaatcttt atcccggaag cgtatgatga ataatcagca
     1801 aaagaaaagg gttgccctgt ttggttctac gggatcggtg ggaacccagg ccctggaagt
     1861 gattgaagcc aatccggagt tgttttccgc ggaagtgctt acggcccatc agaatgcgga
     1921 gttgctgatc aaacaggcga ataagttttt gccggatatc gtggttatcg gtgacgaatc
     1981 caaataccag atggtccgtc aggcgctgga agcacatccc atcaaggtat tttgcggtga
     2041 aaaagcgctg atggaagtcg cttccatgga ttcctatgat gtgatgctgg cggcgatcgt
     2101 cggatttgcc ggacttatgc cgaccctcaa agcgattgag aacgggaaag ccattgccct
     2161 ggccaataag gagaccctgg tggttgccgg agacatcgtg atgcgtaagg cggtggaaaa
     2221 ccgggcgccc atcattccgg tcgattcaga acactctgcc atttttcaat gcctgctggg
     2281 agaaacgcgt aaccgcattg agaaaatcgt gctcacggca tcgggaggac cctttctcgg
     2341 caagaagccc aattttctcg tcaatgtaaa aagagatcac gccctgcagc atccgaactg
     2401 ggccatgggt gcaaagatct ctatcgattc ggccacctta atgaataaag gactggaaat
     2461 gattgaggcc aaatggcttt ttaatctgca gccgcaacag atccaggtgg tgattcatcc
     2521 gcaaagcatc attcacagca tggtgcagtt tgaggacgga agtattaaag cccagatggg
     2581 attgccggat atgaaactgc ccatacagta tgcccttgga tttccgctcc gcttaccgaa
     2641 taaaatgccc aggtattcgt tcaacaaacc ggataaactg agttttgaag atccggatat
     2701 ccgcaccttc cgcaatttgc agctcgctac ggatgcgctg aaaaaaggag ggaatgccgg
     2761 atgtgtgctc aatgcggcca atgaaatagc agtatatgcc tttttaaaga accggatcgg
     2821 gtttctggac atgaccgatc tcatcgaaaa aaccatccag aagataccct ttttagaaca
     2881 acccacgctg gaagattatt ttgacagcga tggggaggcg cgtaacttcg cggcttcttt
     2941 aattaaattg taaatattcc ctaaaacatt gcagggacgc gccctgcggc tgatctttgt
     3001 aaagatgaat aatactccga tcttgttggc gatagactgg gcaaatgccg gcagcaaagc
     3061 ggcccagctg atcctttccc tttcgctgat tgtcatcgtg cacgaattcg gccactacct
     3121 tccggccaag atcttccggt gccgcgttga aaaattcttt ttgttcttcg atccctggtt
     3181 tgcgctggtc aaaaaaaaag tgggagaaac cgtttacgga atcggatggc tgccgctcgg
     3241 cggctatgtg aagattgcgg gcatggtgga cgagagcatg gataaggaac agatgaaaca
     3301 gccgccgcag ccctgggaat tcagaagcaa acccgcctgg caaaggctga tcataatgct
     3361 tggcggtgta accatgaatg tattgatggc ttttgtgatc tatgccatga tcctttttac
     3421 ctggggtgaa aaaaaaatcc cgatgtcggg cattacctac ggcgtatcgg taaccgacag
     3481 cctgatgtat gaaatgggtt tccgaaacgg cgacaaaatt ttgtcggtca atggagaacc
     3541 ggtcacctat ttcgatgacc tgaccaagac actgctgctg ggagaaaacg tggaactgga
     3601 aagagatggc aaaaaacaaa ccatcaacct gcccgtgaac ctgatcggaa aactggttga
     3661 aaaaaagagg accggtcagc ctttttttat tccgcgcatc cccgtgattg cagaccaggt
     3721 tccggatacc tccaatgcgt ataaggccgg tctcagaaga ctggaccaga taaccgccgt
     3781 ggaagggatt gccaccccgt tttatgaaga atataaaaaa gcggtggatg cccataaggg
     3841 acaaagcatc agactgacca tagaccggaa cagcaaaaca gacaccctgc atgcggacgt
     3901 aaatgcggac ggaaccttag gcttttaccg cctgggggac ctggaaaccc tggatagcct
     3961 cggactgatc cggatcgaac gaaaagaata cagcttcttt gcctcattcc cggccggaat
     4021 ccatttggcg ggtgagaccc tgaaatcgta tatcgaccag tttaaaaaag tgctttcccc
     4081 taaaacgggc gcctataaag gagttggggg ttttaaggcc atcggttcca tttttccgtc
     4141 catgtgggac tggcaatcgt tctggaccat caccgcattc ctctctgtca ttctcgcttt
     4201 catgaatatc ctgcccatcc cggcactgga cgggggacac gtattattta ccctgctgga
     4261 aatgatcacc ggccgtaaac ccagccagaa ggtattggaa tatgcccagg tgatcggcat
     4321 ggtgctgctg ctgggtctca tgatctacgc caatggaaac gattggtttg gctggggcag
     4381 gggcaaatag ggcggtacgg cctttattcc gccacttaca tactcatttc tgcaatctac
     4441 acaatccggt tccggaccgt ttttgcaaat cctaatttgt attggatttt ggactgcatg
     4501 ccatcaaatg gccattaacc ttaaaccttt actacgatga agcaggaaag cacgaccggg
     4561 ttttattata taggcaccca atccgcccac atggcgaaat tggtaagtat atataagcgc
     4621 ggttttgcgt ccgacaacat gcagcaggca atcacggagc tgacccgtga aaaacactct
     4681 ccgcctgaag ttatatgctg cgaggcccgg tttggcctcg cagcaataac tcgttttgcc
     4741 ggtttcctgg ccaaacatcc cacgctctcg cggattcctt ttatcctcga tgcggaccgg
     4801 atgacaaccg gagaccatga ggagtatatt aaaaacaaac tggcagacga aatcttattc
     4861 ctccgggatt acgatgaaaa tctgctgagc cggaagatcc ggttcctgca aaatttcaaa
     4921 accaaaacaa cggagctgga caaattggaa aaagcggaga tctctgcgca ggttccggct
     4981 tccctgtata attttttaaa acgttctttt gaaattctgg tcgctttatt cctgttattc
     5041 acactggccc ctctttttct gctgatcgct atcgcgctga aactggaatc cggggggccg
     5101 gttttttatg tttcaaaaag ggcaggtaaa ggataccgga tctttaattt ttataagttc
     5161 aggacgatgt acgatggatc ggatcagcat atggttgaat tagcgcacct caatcagtac
     5221 cggggttccc gggaaccccg gttttttaaa gtgaaaaatg atccccggat aacccggatc
     5281 ggacagttcc tccggaatac cagtctcgac gaattgccgc agctcatcaa tgtattgatc
     5341 ggagatatgt cgctggttgg caatcgcccc cttccgctgt acgaggcgga aaccctgacg
     5401 acggatgccc tggccaaaag atttcttgca ccggcgggca ttaccggcct ctggcagatt
     5461 aaaaaaagag gtaagcaaaa aatgacggag gaagaacgga tcagcctgga tattgaatac
     5521 gcggataatt ccaatttttt atatgattta tggattattg ccaacaccgc acaggccatg
     5581 atccagcggg aaaatgtata aaagggttat cttagaaaaa acgcagccgg ttaatgattg
     5641 cacgtacggg tcacaaatat tcatttcttt atgccataag ggaattcctg ttcccctgca
     5701 tccgcctgcg cgtcctttta gtttgtcttt ttctaactgg ttcccactgg ttgccggcac
     5761 agtccgctta tgtggacagc atgatccggt gggctgaatc acacccccgg accgattcgc
     5821 agaagatccg taccctccac cgcatcagtt atatgctaag cgaaacggat gtcaacagat
     5881 ccttcgccta ttacgaacag gtctccagcc tgagcgattc ccttcatttt atttttggca
     5941 aggcgctcgc cagcatcaat ctgggaatcc tgctttcttc tgcgggtaat tttgaatcca
     6001 gcaccaaagc atttttcaat gcgatcgacc tggcggattc ctgtgcggcc acccgcgtaa
     6061 aggccgttgc cctgaacaat atcggagaaa attttttctc cctgagggag tataacaaat
     6121 gccgcagtta tgccaggcag gccattgata tcaacacccg ggtcagggcc tggcgcggcg
     6181 tagccatcaa ttatgaactg atgcagcgct gtgattttga acagggccgc tattcagatg
     6241 ccagggagaa cctggataag ggcatgccct atgcgcgcct ggccaatgag aattatattt
     6301 attccccctt ttacctggga tacggaaaac tcaaggcagt cagcaaccag aaagattcgg
     6361 ccgggttttt cttcgacaag gcgattacaa gcgcccggca ggacgccgat ctccggaatg
     6421 agtaccaggt gtacctcgcc aaagcaaaat accagaaatt ccttccggtc cggaataaaa
     6481 tcgagctgct gtcaaaagcc ctggggctgg ctaaccagac ccattttgaa gagggacgag
     6541 ctaaggcggc ccaggaatta tcgtccatgt atgataacct gaaccgggaa gactcctccc
     6601 tgttctatta ccgcatgtac cggtccgcca ccgattctct cttttccgaa aagaacaggc
     6661 gtaatatgat tgtcaatgaa tcggaatggg tggtcaaaag aaaagaactg gaaaacagta
     6721 atctgaagga gctggcggcg gtgcagaaaa aacagatcgc cttcaagaat atcctgcttt
     6781 tctttatcgg tatggggttc gtgctgaccc tgctggtcgc ttttctcatt tataaatcca
     6841 ttcagtccaa aaagttgaaa gaggaattgg catacaagca aaagatcgct gaaacggaga
     6901 tgcaggcatt aagggcccag atgaatcccc atttcttttt taacagcctt aacagtattg
     6961 agaatttcat tatgcagaat gaaaagaaac tggcctcaga ctacctgaat aaatttgccc
     7021 ggttcatccg gagcatcctc gacagcagtt atagtgaact gattgaaata aaaaaagatg
     7081 tcgaatcatt gcagctctac atcgacctgg aacggctgcg ttacaatgat aaattcagat
     7141 tttgctgccg ggtcgatccc ctcctgatcg atggggaata tcatgtgcct tccctgctgg
     7201 tgcagccctt cctggaaaat gccattattc acggaatcgg cccgagcgac cgcaatgacc
     7261 tgaaattaac cttgctgatc aggctgaaag attccgggat acagtatgtc attgaagata
     7321 acggtattgg ccgtgagcaa tccagggcat ataaagacct gaacaaaccg ttccacaaaa
     7381 gcgtcggcat gaagatcacc caggatagaa taaatatatt taatgaggat gcacccgccg
     7441 cggacagcgt aaaaataatt gatctttatg atgatcagca aagaccttcc ggtacccgga
     7501 tagaatttac cattaaatta gtgtcgtatg caagccctaa aagccgtatt ggttga
//
LOCUS       DASYCU010000581         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2736475, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000581 DASYCU010000000
VERSION     DASYCU010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2736475"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /gene="rpsM"
                     /locus_tag="VGM24_11730"
     CDS             <1..318
                     /gene="rpsM"
                     /locus_tag="VGM24_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY4156096.1"
                     /translation="IYGVGPSTSKYILSKAGISLDKKVSEWNDDELNAIRNIITNELK
                     VEGALRSEVQMSIKRLLDIACYRGVRHRKGLPVRGQRTRTNSRTRKGKRRTVAGKKKA
                     PKK"
     gene            460..867
                     /gene="rpsK"
                     /locus_tag="VGM24_11735"
     CDS             460..867
                     /gene="rpsK"
                     /locus_tag="VGM24_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY4156097.1"
                     /translation="MAKAQQQTSAKAAAKKRVVKVDAYGDAHVSASFNNIIISITNKN
                     GQLISWSSAGKMGFKGSKKNTPYAAQMASADAAKTALDAGLKRVDVYVKGPGSGRESA
                     IRSLANNGIEVVMIKDVTPLPHNGCRPPKKRRV"
     gene            1022..1627
                     /gene="rpsD"
                     /locus_tag="VGM24_11740"
     CDS             1022..1627
                     /gene="rpsD"
                     /locus_tag="VGM24_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY4156098.1"
                     /translation="MARYIGPKTKISRKFGEPILGNGKWLDKNSNPPGQHGATKKRKT
                     LGEYALQLKEKQKAKYTYGVLERQFRKTFEEASRRKGVTGENLIRLLEARLDNTVFRL
                     GISPSRPGARQLVSHKHITVNGEVLNIPSYQLKPGDVIGLKPTSQSNAGITSAARGKG
                     NKINWLDWNEAELKGVFIAYPERESVPENIKEQLIVELYSK"
     gene            1664..>1966
                     /locus_tag="VGM24_11745"
     CDS             1664..>1966
                     /locus_tag="VGM24_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY4156099.1"
                     /translation="MAILNFQKPDKIILQKATDFEAQFEFRPLEPGYGVTIGNALRRV
                     LLNSLEGYAIIGVKIEGADHEFATLKGVTEDVVEIILNLKQVRFKKKVDHEVAQEKI"
BASE COUNT          560 a          490 c          516 g          400 t
ORIGIN      
        1 atctatgggg ttggtccttc cacatcgaaa tacattttgt ccaaggctgg tatcagtctg
       61 gataaaaaag tttccgaatg gaacgatgac gagctgaacg ccatccggaa catcatcacg
      121 aacgaactga aggtggaagg cgcgctccgt tctgaagtcc agatgagcat caaacgcctg
      181 ctggatatcg cctgttaccg cggtgtccgt caccgtaaag gcctgcccgt gcgtggtcag
      241 cgtacccgca ccaacagccg tacccggaaa ggaaaacgca gaacggtggc cggtaagaaa
      301 aaggcgccca agaagtaatt tgaccggcat cctgtgcggg atgctttggc ttatagcacc
      361 aaattcagaa tcaactacgg ataccgggtg cgggtggagg gttgattcga tcccgggcag
      421 accgggaaaa ttaaaaaacc agattaccta tttcgaacaa tggcaaaagc acaacagcag
      481 accagcgcca aagcagctgc aaaaaaaaga gtggttaagg tagacgccta cggcgatgcc
      541 cacgtatccg caagtttcaa caacattatc atcagcatta ccaacaagaa cggacagctg
      601 atttcctgga gcagcgccgg caagatgggt ttcaagggat caaagaagaa tacgccctat
      661 gcggcccaga tggcttctgc ggatgcagcc aagacggccc tggatgcggg gttgaaaaga
      721 gtggacgttt atgtcaaggg tccgggttcc ggtcgcgaaa gcgccatccg ctccctggcc
      781 aacaacggta tcgaagtggt gatgatcaag gatgtcaccc ccctgccgca caacggttgc
      841 cgtcctccga agaagagaag ggtttaaatg acttgccgct gttgccgcgc cgcttttcaa
      901 aacgggctgc cggcatcgga ggtggtatta tcggttcaat atattttatc aagctgggca
      961 ggattccatt ttaagatttt tcataatcct gtccgggtac tcaaaaatct gaaacttatt
     1021 tatggcacgt tacatcggtc cgaagaccaa gatctcccgc aaattcggtg agcccatttt
     1081 aggcaatggc aagtggctgg acaagaacag caatccgccc ggccagcatg gagcaaccaa
     1141 aaaacgcaaa acgctgggtg aatacgccct gcagctaaag gaaaagcaaa aggccaagta
     1201 cacctacggc gtgctcgaac ggcagttccg gaaaactttt gaagaagcgt cccgccgcaa
     1261 gggggttacc ggtgaaaacc tcatccgcct cctggaagcc aggctcgaca ataccgtatt
     1321 ccggctgggt atttcccctt cacggcccgg agcgcgtcag ctggtttcgc acaagcacat
     1381 taccgttaac ggggaagtgc tcaacattcc ttcttaccag ctgaagcccg gagatgtgat
     1441 cggtctgaaa cccacctccc agagcaatgc cggcatcacc agcgcggccc ggggaaaagg
     1501 caacaagatc aactggctgg actggaatga agcggaactg aaaggtgtgt tcatcgctta
     1561 tcctgaaagg gagagcgttc ctgaaaacat caaggagcag ctgattgtag agttgtacag
     1621 taaataagcg tattatcatt cgtaaatcat cgaaaattta aatatggcca ttttaaattt
     1681 ccagaaacca gacaaaatca ttcttcaaaa agcgactgat ttcgaagccc agttcgaatt
     1741 tcgtcccctg gaacccggtt atggggtaac cattggaaat gccctgcgca gggtactgct
     1801 gaattccctg gaagggtatg ccatcatcgg tgtaaaaatc gaaggggctg accacgaatt
     1861 tgccaccctg aaaggggtga cggaagacgt ggtggaaatc atcctcaacc tgaagcaggt
     1921 ccgtttcaag aaaaaagtgg accacgaagt tgcccaggaa aaaatt
//
LOCUS       DASYCU010000582         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4332956, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000582 DASYCU010000000
VERSION     DASYCU010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4332956"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VGM24_11750"
     CDS             complement(<1..802)
                     /locus_tag="VGM24_11750"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY4156100.1"
                     /translation="MKVAVVGATGLVGTKMLQILEERNFPVSELIPVASEKSVGKKVR
                     FKGKEYPVMSATDAIAAKPAVALFSAGGATSLEWAPKFAAAGITVIDNSSAWRMDPAK
                     KLVVPELNADVLGKEDKIIANPNCSTIQMVLALNPLHKKYKVRRIVVSTYQSVTGTGV
                     KAVNQLLNERKGVAGEMAYRYPIDMNAIPQIDIFLDNGYTKEEMKMLNETRKIMRDDS
                     IRVTATTVRIPVMGGHSESVNVEFDRDFDLGDVKQLLAAAEGVVVVDDP"
     gene            1021..>1597
                     /locus_tag="VGM24_11755"
     CDS             1021..>1597
                     /locus_tag="VGM24_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY4156101.1"
                     /translation="MAIRRLLLVLFLLRFYFPARSQSAGQYAVLIDEFLADPAPSAGL
                     PSSSFIELKNVSGFSVNLHNWKIGTGSSTATIKTEYLLGADSFLILCPSSAEAAYRPF
                     GPVLGITGFPSLNHDAGEIVLSSGEGAVIHAVQYDKSWYHNELKSDGGWTLEMIDLKN
                     PCSGFSNWTASSGANGGTPGKPNSVEAENPDQ"
BASE COUNT          336 a          443 c          377 g          441 t
ORIGIN      
        1 ccgggtcgtc caccaccacc acgccttccg ccgcggccag cagttgcttt acgtcgccga
       61 gatcaaaatc ccggtcaaat tcaacgttca cggattcact gtgtccgccc attaccggaa
      121 tacgcacggt ggtggctgtt acgcggatcg aatcatcccg catgatcttg cgcgtttcat
      181 tcagcatttt catttcttct ttggtatacc cgttgtccag gaaaatatcg atctgcggaa
      241 tcgcattcat atcgatggga tagcggtagg ccatttctcc ggcaactcct ttgcgttcat
      301 tcagtaattg attcaccgcc ttcacgccgg tgccggtaac gctctggtag gtgctcacca
      361 cgatccttct gaccttatat tttttatgca gcggatttaa ggccagcacc atctggatgg
      421 tggaacagtt ggggttggcg atgatcttat cttcttttcc cagcacatcg gcattcagtt
      481 ccggaaccac cagttttttt gccggatcca ttctccaggc cgaggaattg tcgatgaccg
      541 taatgcccgc ggccgcaaat ttcggcgccc attccagaga ggttgccccg cctgctgaaa
      601 acaaagccac ggccggctta gccgcaatgg catcggttgc gctcatcacc gggtattcct
      661 ttcctttaaa ccggaccttc tttcccacgg atttttcgga ggccacggga atcagttcac
      721 ttacgggaaa attcctttct tccagaatct gcaacatttt tgttcctacc agtccggtag
      781 cgccgacaac tgcgactttc attggttttg ttttgatgtt ttaactgttt tcaaatcaaa
      841 aagcctcccc gatccgggaa ggcttttatt atcttggtat acaaaacaat caggcacctt
      901 ccccgctcca gggcatcttt atgctgtgct ttgtatatgt ggtaatcacg ttcatcgagg
      961 gcgtaaaaat aacctccaat tggctggcag ccaaattatt tttacagaaa tgttttcccg
     1021 atggctattc gccggcttct ccttgttttg ttcctgctcc gcttttattt tcctgcccgt
     1081 tcgcaaagcg cagggcaata tgccgtgctt atcgatgagt tcctcgcgga tcccgcacct
     1141 tccgccggct tgccgtcttc ttcctttatt gaactgaaga atgtttccgg cttttccgtt
     1201 aacctgcata actggaagat cggcaccggc tcctcaacgg caaccattaa aacggaatac
     1261 ctgctgggcg cggacagttt tctgatcctt tgtccctcct ccgcggaagc tgcctaccgg
     1321 cctttcgggc ctgtcctggg aattaccggt ttcccttcgc tgaatcatga tgccggtgaa
     1381 atcgttctga gttccggaga aggagcggtt atccatgccg ttcagtatga taaaagctgg
     1441 tatcacaacg agctgaaatc ggatggcggc tggacccttg aaatgatcga tctgaaaaat
     1501 ccatgcagcg gattttccaa ctggacggcg agcagcgggg caaacggtgg cacgccggga
     1561 aaaccaaatt ccgtggaggc agagaacccg gatcagg
//
LOCUS       DASYCU010000583         4044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1868004, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000583 DASYCU010000000
VERSION     DASYCU010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868004"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VGM24_11760"
     CDS             complement(<1..334)
                     /locus_tag="VGM24_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY4156102.1"
                     /translation="MIFLTGGTGFTGSYILAELVRQGHAVRALRRKRAIPFYLSREIV
                     EKVEWVAGDILDVAGLEEQLKGCNRVIHAAGLVSFYPEDKKKLFKINIEGTANLVNAA
                     LENNITDFI"
     gene            complement(370..1218)
                     /locus_tag="VGM24_11765"
     CDS             complement(370..1218)
                     /locus_tag="VGM24_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY4156103.1"
                     /translation="MTDSFCICGAGTMGSGITLSAAQHGIPVILYDLNPEMISRASRQ
                     IEAELQQQADKKRITDKEKNEILHRIRFTTSLSECRAGIILEAIIENIEAKSILFTGL
                     EKINDSSTLFASNTSSLSINALAEQTGFPERIIGMHFFNPASRMKLVEIIKTGHLSEE
                     TIQRIRKLALRMGKTPVLCNDSPGFIVNRVARPFYLEALRLAEKGIADIDSIDKLMEA
                     AGFKMGPFHLMDLIGNDINYTVSCSVYEATGKPARLKPSSLQELKVKQGALGKKTGKG
                     FFEWGN"
     gene            1301..1684
                     /locus_tag="VGM24_11770"
     CDS             1301..1684
                     /locus_tag="VGM24_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY4156104.1"
                     /translation="MKKKIINTPDAPAPIGPYSQAVQVDQMLFLSGQVAINPQTGNLE
                     TADIQTETRQVMNNLKAVLQAAGMDFSQVVKTSIFLSDMTWFAAVNEAYGKYFSSDFP
                     ARETIAVKTLPKGVNVEISMIAVLG"
     gene            complement(1695..2882)
                     /gene="kbl"
                     /locus_tag="VGM24_11775"
     CDS             complement(1695..2882)
                     /gene="kbl"
                     /locus_tag="VGM24_11775"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY4156105.1"
                     /translation="MNEIFVQRIAGQLEEIRSAGLFKTERIISSPQGVEITANGQRVL
                     NFCANNYLGLSSQPRVIGAAHKVLDERGYGLSSVRFICGTQDIHKELEKKLSAFLGMD
                     DCLLYAAAFDANGGVFEPLFGEEDSIISDELNHASIIDGVRLCKAKRLRYKHNDMPDL
                     EEQLRLSMSSRNRIIVTDGSFSMDGTIAQLDKICDLADRYNAIVMSDESHSTGFLGKT
                     GRGTHEYRNVMGRMDIITGTLGKALGGANGGFTSGRKEIIELLRQRSRPYLFSNSLAP
                     CIVGASIAILDMLEESTALRDRLEFNTKYFRARMLEAGFEIKPGDHPIVPIMLYDAVI
                     AQEFAAKLLEEGIYVTGFFFPVVPKGQARIRVQLSAAHEKEQLDQAVEAFVKVGKALK
                     VIR"
     gene            2949..3485
                     /locus_tag="VGM24_11780"
     CDS             2949..3485
                     /locus_tag="VGM24_11780"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4156106.1"
                     /translation="MRGITVIHHGDNNRERLMTAEDYNDCVKHYADGLYRFMLKSTKQ
                     VEDARDLVQSAFEKLWEHREEVNALKSKSYLFTIAYHKMIDLNRKNRWVDFRESLPEY
                     TETERGPTPRLRQVLEEAMNRLTERQRSLVMLKDYEGYTYEEIGEITGLNAGQVKITL
                     HRARIQLKQYLGSVENVL"
     gene            3496..>4044
                     /locus_tag="VGM24_11785"
     CDS             3496..>4044
                     /locus_tag="VGM24_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156107.1"
                     /translation="MIINRNNYEEYFLLYIDRELNRDERMMVDDFVVRHPDLEKEFNA
                     LKQTIAAAPRMVYEGKEKLLKENKRRIYPVYWMRLAATLIILLAGGWFLLLVTGKTRR
                     PDGPVSPSKEVARRAPVRPQGNPSNMANPVPPAKKAVQSGNKENPVAAANPKTQPVSQ
                     QVLSSKEAGADPVKNSSDEKGSA"
BASE COUNT         1035 a         1027 c         1006 g          976 t
ORIGIN      
        1 agatgaagtc cgttatatta ttttccaatg cggcgttcac caggttagcc gttccttcaa
       61 tatttatttt gaataatttc tttttatcct ccggataaaa ggaaaccagc ccggccgcat
      121 ggatgacccg gttgcaccct ttgagctgtt cctccagccc ggccacatcc aggatatctc
      181 cggcaaccca ttccactttt tcaacgatct cccgggataa atagaagggg atggcgcgtt
      241 tgcggcgtaa agcgcggacg gcatgtcctt gccggacgag ctcagcgaga atataggatc
      301 cggtgaaacc ggtgccgccg gtgaggaaga tcattgcgta aaagtggaat taatttctac
      361 attttccaat taattccccc actcaaaaaa ccctttcccc gtcttctttc ccaatgcccc
      421 ctgtttcact tttaattcct ggagggaaga gggttttaac cgggcaggtt ttccggtcgc
      481 ttcataaaca gaacagctga cggtataatt tatatcgttt ccgatcaggt ccatcagatg
      541 gaagggcccc attttaaaac cggctgcctc catcagtttg tcaatgctgt cgatatctgc
      601 gattcctttt tctgccagtc tcaacgcttc cagataaaat ggccgcgcca cccggttaac
      661 gataaagccg gggctgtcgt tgcaaagcac gggcgtcttt cccatccgga gggccagctt
      721 gcggatgcgt tgaatcgtct cttcggaaag atgacccgtt tttatgatct cgaccagttt
      781 catgcggctt gccgggttga aaaaatgcat tccgataatc cgttccggga aaccggtttg
      841 ttccgcaagg gcatttatcg aaagggagga agtgttgctt gcgaaaaggg tcgaggaatc
      901 atttattttt tccagcccgg taaaaagtat gctcttggcc tctatattct caatgatggc
      961 ttccaggatg atccctgcgc ggcattccga taatgaggtg gtaaaccgga tgcgatgcag
     1021 aatttcattt ttctctttat ccgttatgcg ctttttgtct gcctgctgct gcagctccgc
     1081 ttcaatctgc cgggaagccc tggaaatcat ttccgggtta aggtcatata atatcacggg
     1141 aatgccatgc tgcgccgccg acagggtgat acccgatccc atggttccgg ctccgcatat
     1201 acagaaagaa tcagtcattc cggaattatt aatgactaaa gatacgcatg ccgggcgttc
     1261 gtcccgaagg gaatccggcc ccgatcctta catttgaaga atgaaaaaga aaatcatcaa
     1321 tacgcccgat gcaccggcgc ccattgggcc ttatagccag gccgtgcagg ttgatcaaat
     1381 gttgtttctt tccggacagg tcgccatcaa tccccaaaca ggaaatctgg aaacagccga
     1441 tatccaaaca gaaacccgcc aggtgatgaa taacctgaaa gcggtattgc aggccgccgg
     1501 aatggatttc agccaggtgg tgaaaaccag catctttcta tccgatatga cctggtttgc
     1561 tgccgtaaat gaggcctacg gaaaatattt cagcagcgat tttccggccc gggaaacgat
     1621 tgccgtgaaa acgcttccaa agggcgttaa tgtagaaatc agcatgattg ccgttttggg
     1681 ctgaccgcta tccgtcaacg gataaccttc aaggctttcc ccactttaac aaacgcctcc
     1741 acggcctgat ccagctgttc tttctcatgg gcggcactca gctgtacccg gatgcgggcc
     1801 tgtcctttag gaacgaccgg gaagaaaaag ccggtaacat aaattccttc ctccagtaat
     1861 ttagcggcaa actcctgcgc aataaccgca tcgtaaagca tgatcggaac aatgggatgg
     1921 tcgccaggtt tgatttcgaa tccggcttcc agcatcctgg cgcggaaata ttttgtattg
     1981 aattcgaggc ggtcacgcag cgcggtgctt tcttccagca tgtccaggat ggcaatggaa
     2041 gcccccacga tgcagggggc gaggctgttc gaaaacaaat agggccggct tcgctgacga
     2101 agtaattcaa tgatctcttt tcttccgctg gtaaagccgc cattggctcc tcccagcgcc
     2161 ttgcccaggg ttcccgtaat aatatccatc cggcccatga cattccggta ttcatgtgtg
     2221 ccgcggccgg tctttcccag aaatcccgtg gagtgggatt catcactcat cacaatcgca
     2281 ttatagcggt ctgccagatc gcagatctta tcgagctggg caatggtccc gtccatgctg
     2341 aaagaaccat cggtaacgat aatccggttg cgggaagaca tggacaggcg gagctgctct
     2401 tccaggtccg gcatatcatt atgtttatac cgcagccttt ttgccttgca taaccggacg
     2461 ccatcgataa tggatgcatg gttcagttca tcggaaatga tcgaatcttc ctcaccgaac
     2521 aggggttcga acacaccgcc attggcatca aatgcagctg catacagcag gcagtcatcc
     2581 atgcccagga aggccgacag ctttttttcg agttctttgt gaatatcctg cgttccgcag
     2641 ataaaccgca cactgcttaa tccgtaaccc cgctcatcca ggactttatg cgccgccccg
     2701 atcacccggg gctgggaaga aagtcccagg taattattgg cacagaaatt caaaacacgc
     2761 tggccattcg ccgtaatttc aaccccttgc ggactgctga ttatccgttc tgtcttaaac
     2821 aatccggctg accggatctc ctccagctgg ccggcgatgc gctgaacaaa tatctcattc
     2881 atatataata tttttgaaga ttgttgattt gcctggtgtg cgatatataa agataaggca
     2941 tgtaactttt gaggggtata accgtaatac atcatggaga caataaccgg gaacggctta
     3001 tgacggcgga ggactacaat gattgtgtaa aacattatgc agatgggctc taccggttca
     3061 tgctgaaatc cacgaaacag gtggaagacg cgcgggacct ggtccagtct gcttttgaaa
     3121 agctatggga acaccgggaa gaagtgaatg ccctgaagag taaatcctac ctctttacca
     3181 ttgcttacca taaaatgatc gatctgaacc ggaagaaccg ttgggtggat ttccgggagt
     3241 cccttcctga atataccgaa acggagcgtg gcccgacccc gaggttgcgg caggttctgg
     3301 aagaggcgat gaacaggttg accgaacgcc aaagatccct cgtcatgctc aaggattatg
     3361 aaggatatac gtatgaagag atcggggaaa tcaccggatt gaatgcaggc caggtaaaaa
     3421 taaccctgca ccgggcaagg atccagctga aacaatatct cggaagtgtg gaaaatgtgc
     3481 tttaaataaa atatcatgat catcaaccgg aataattacg aggaatattt cctgctctat
     3541 atcgaccggg aactgaatcg cgatgagcgg atgatggtgg atgattttgt tgtccggcat
     3601 ccggatctgg aaaaggagtt taacgcgctg aaacaaacca tcgccgctgc tccccggatg
     3661 gtctatgagg gcaaagaaaa actcttaaaa gaaaataaac ggcgcattta tccggtctac
     3721 tggatgcgcc tggcggcgac gctgattatt cttttggcgg gaggatggtt cctgctgctg
     3781 gtgaccggaa aaacccggag accagatggc ccggtcagcc cctcaaaaga agttgcacgc
     3841 cgtgcgcctg tccgcccgca gggaaatccg tcgaatatgg cgaacccggt tccccccgca
     3901 aaaaaagctg tccaatccgg aaataaagag aacccggtgg ctgccgccaa tccgaaaacc
     3961 caaccggtga gccagcaggt cttaagttca aaggaagccg gtgcggatcc ggtaaaaaac
     4021 agttcggacg aaaaaggatc tgca
//
LOCUS       DASYCU010000584         6092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4185776, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000584 DASYCU010000000
VERSION     DASYCU010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4185776"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /locus_tag="VGM24_11790"
     CDS             complement(<1..1976)
                     /locus_tag="VGM24_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4156108.1"
                     /translation="MSGKVFFMPLLFLVILSFKAYERDPPSFRAISRQFAKADSLFNL
                     TNTTSITDSAALEEFRQVMEQSAAFGRSAGTDSIFFQSFYKAGVLYEVSGKFRLATLA
                     YLQALVYAADERERLKMYVFAGAGYYYQNNFDSANYFLLKAEEDAGNQAGSEDLVRLY
                     NTLGVLYYDNGNYLQSKNYFNQALSIIRKKNPADRVNALSVQLNMATCFYRLGLYDQA
                     LQIYQGATRQKLFLNQIYMNMGRAYAGLRRYPQALTAFRQVQVEKLPAVLNEMARVAL
                     ESGHSDSAQRWLDLFRSEKEHLKVNMLDAGINMLYSGDLDLYRSDPESALKHLQEAVI
                     LFSGNFKDPLIRHNPTGFTGSFAYYTLFDALYKKATAWELQYKKTSRPEDLQSAFDTY
                     QSTLALLTYIERSYEMDDAKILLKQKSAQVYKNALQVCFDLYQLYPDSHYLEDAFIIS
                     ERNKASVMASNLREKNFHFSSDQEDELVSQERNIKFNIARLSIKSDQEPEAGTLEKIN
                     ADKAAYESRLAAVQKQLEKDSRYYQLKYQDDYPSIRKLQTSLNANQAMISLSNTPEAV
                     HVFVVTRSSFRHIKLDSGSAIRENVGEWIRILQNTENGKHAATRKLGQLLYTQLIKPL
                     EGLAGDKEDWIVVPDGIFFLLPFESLPADASGTL"
     gene            complement(2041..2985)
                     /locus_tag="VGM24_11795"
     CDS             complement(2041..2985)
                     /locus_tag="VGM24_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156109.1"
                     /translation="MKKNLHPGSTILILALLCLFACKKTINSTEDVPYKPPVKVDSTK
                     NKVDSLQPPKDSVQPAPKDTTSVPFPQVPLLGCTYSPDYGDSIVFTQPTNGSDYIIYP
                     VNNSGIAGTYFSWPGGLVLNSHTGAINMTKSETGARYAVGFVKSGTTDTCLTQLIVGG
                     AAYMDSVYVLGDNQTTAAPYYNADPYAPPVCTGSSGGQGCQFDYNNHAKNQGVVIDQH
                     TGIINLQQTMKNSLFGLLPIDGTTINTVVYYKLNDNSNSAPQQIGLKLMFYNKKSDIP
                     PSLLASVVDNLLNVLQNLLISKGSSSPRPPLIIITRYN"
     gene            complement(3214..4347)
                     /locus_tag="VGM24_11800"
     CDS             complement(3214..4347)
                     /locus_tag="VGM24_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY4156110.1"
                     /translation="MISPAYKLPEKNLPAGGRLISLDFYRGLIMVLLMLESTRLYGYL
                     GEGAKGGFLEPLINQFFHHPWHGLHFWDLIQPGFMFIAGVAMAYSIHKQKAQGVPWKS
                     RAGKALRRSGWLFFWGVLDYSVRGDHLSFELWDVLTQLSFTMLVAFFIFNWKTGYQIL
                     FSVGLLLLTECLYRFSHFPGFDQPFTDQHNFGNYVDLILMNKINPGGWVAINCIPTAA
                     HTIWGAVAGKWLIELRDNKSRIRRILIFAGAALVCGYLLDFCLITPIIKRIATSSFTL
                     VSGGYCLLALACLYGWIDVLGHKRFLKFFLIVGMNSIFIYVFFEIVVDRWLYDYMHTL
                     VSGIISPFALSPLPVEVMVSLAVFILEWGLCFWLYRKKIFFKL"
     gene            4534..>6092
                     /locus_tag="VGM24_11805"
     CDS             4534..>6092
                     /locus_tag="VGM24_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY4156111.1"
                     /translation="MFTSMTTPATGLICWSFLISALVCSCNPRQRLFKKIPSAVSGIH
                     FNNEIIENDSINPIDKEFLYNGGGVAVGDFNNDGLQDLYFTGSMVSNKLYLNQGNFRF
                     RDITRAAGVTGDGRWCSGVSVVDINNDGLLDIYVCTTIKNDPAERTNLLYINQGMDKK
                     GIPIFKEMAAEYGLADTSYSVQAAFFDYDNDGDLDMYLVTTKMAQRDAAQFASNSINR
                     DSADVDKLFRNDWDARLGHPVFTDVSRQAGITHQGYGLGVSIVDINRDGWKDIYVTND
                     FYGSDQLYINNKNGTFSDRVSSYFKHTSQNAMGNDIADINNDGLADVLTLDMDPPGNF
                     RKKKNMSAGNYSIYQHMANGNYMMQYVRNTLQLNQGPQVTGTDSIGNPVFSDIGFYAG
                     VAETDWSWNASLADFDNDGNRDIFITNGYPKDITDHDFAAFRRQASDLLSKKSLSAQI
                     PQIKIPNCVFKNKGNLQFENMTIPWGLEEPSFSNGAVYADLDNDGDLDYVINNINGEA
                     FVYENSTITGK"
BASE COUNT         1680 a         1422 c         1471 g         1519 t
ORIGIN      
        1 atcaatgtac cggaagcatc cgccggcagc gattcgaaag gaagaagaaa gaatatcccg
       61 tccggcacca cgatccagtc ttccttatcc ccggcaagac cctccaacgg tttgatcagt
      121 tgtgtatata agagctgccc cagttttctt gtggcggcat gttttccgtt ttccgtattt
      181 tgcaaaatcc ggatccattc acccacattc tcccgaatag cagatccgct gtcaagcttt
      241 atatgccgga atgaactccg cgtgaccaca aacacgtgca cggcttccgg ggtattgctc
      301 aaactgatca tggcctggtt tgcattcagg ctggtttgaa gtttccggat ggagggataa
      361 tcatcctggt attttaactg ataataccgg ctgtccttct ccagctgttt ctgaaccgct
      421 gccaggcggg attcataagc ggccttatcc gcattaatct tttccagcgt tccggcttcg
      481 ggctcctggt cggactttat acttaaccgg gcaatgttga attttatatt tctttcctgg
      541 ctcaccagtt catcttcctg atccgatgaa aaatgaaaat tcttttccct caaattagaa
      601 gccattacgg aagccttatt tctttcgctg ataataaaag catcttcgag gtaatgacta
      661 tccggataaa gctggtatag atcgaagcag acctgaaggg catttttata cacctgggca
      721 cttttttgct tgagcaggat cttggcatca tccatttcat aactcctttc aatataggta
      781 agcagggcta atgtgctttg atacgtgtca aaggccgatt gcaggtcctc cggtcgggaa
      841 gtttttttat attgtaattc ccaggccgtt gccttcttgt acaaggcatc aaaaagggta
      901 taataggcga aactgcccgt aaagcctgtt ggattatgcc gtatcagggg gtccttaaaa
      961 ttgccggaga agagaattac cgcttcctgc aggtgtttga gcgcggattc gggatcagac
     1021 cggtaaagat ccagatcacc actataaagc atattgattc cggcgtccag catatttact
     1081 ttcagatgtt ctttttccga ccggaacaga tccagccagc gctgggccga atcactgtga
     1141 ccggattcca gggccaccct ggccatttca ttcaacacgg caggaagctt ttccacctgc
     1201 acctgccgga atgccgtgag cgcctgggga taacgccgga ggcctgcata ggcccttccc
     1261 atattcatat agatctggtt gaggaaaagc ttctggcggg ttgccccctg atatatttgc
     1321 aaggcctggt cgtacaggcc gagccggtaa aaacaggtgg ccatattcaa ttgcacgctc
     1381 aatgcattga cccggtccgc aggatttttt ttccgaataa tgctgagcgc ctggttaaaa
     1441 taattcttgc tctggaggta attgccatta tcataataca gcacacccag ggtattgtac
     1501 aaacggacca ggtcttctga accggcctgg ttccccgcgt cctcctccgc tttcaaaaga
     1561 aaataatttg ccgaatcaaa attgttctga tagtagtatc ctgcacccgc aaatacatac
     1621 attttcagcc tttccctctc atcggcggca tagacgagtg cctggaggta ggccagggta
     1681 gccagcctga atttcccgct tacttcatac aggacgcctg ctttataaaa agactggaag
     1741 aaaatactgt ccgttccggc cgatcttcca aaggcagcgg attgttccat cacctgccgg
     1801 aactcttcca gggcagcact atcggtaatt gaggtggtat tggttaaatt gaaaagactg
     1861 tcggcctttg caaattgcct gctgatggcc cggaatgaag gcggatcccg ttcgtatgcc
     1921 ttaaaggaaa ggatgaccag gaaaagcagg ggcataaaaa aaactttccc ggacataagc
     1981 tgaatttcaa tggattcaga tttaacgtcc gggaaagggc aatattgtcg gggatacggg
     2041 ctagttgtac cgcgtaatga taatcagcgg cggcctggga gaactgctgc ctttacttat
     2101 cagcaggttc tgcaggacgt tcaacaggtt atcgaccacg gatgccaaaa gggatggagg
     2161 aatatccgat tttttattgt agaacatcag tttcaggcca atctgctgtg gggcgctgtt
     2221 gctattgtcg ttcagtttat aatagaccac ggtatttatc gtcgttccgt cgatcggaag
     2281 aagtccgaaa agactgtttt tcatggtctg ttgcaggtta atgattccgg tgtgctggtc
     2341 aatcacgaca ccctggtttt ttgcgtgatt attgtaatcg aactgacaac cctgaccacc
     2401 ggagcttcct gtacaaaccg gcggagcgta aggatctgca ttataataag gagcagccgt
     2461 ggtctggtta tcgcccagta catacacgct gtccatatag gcggctcccc ctacgatgag
     2521 ctgggttaaa caggtatccg tggttccgga ctttacaaat ccgaccgcgt aacgggcacc
     2581 ggtctcactt tttgtcatat tgatggcccc ggtatgggaa ttcaggacga gcccgccggg
     2641 ccatgaaaaa tacgtcccgg caatccctga attattaacc ggatagataa tataatcact
     2701 tccgtttgtg ggctgggtga acacgatgga atccccataa tcgggagaat aggtacatcc
     2761 cagtaaaggc acctggggaa agggaacact ggtggtatcc tttggagctg gctgtacgct
     2821 gtccttcggg ggctgcaggg aatccacctt attcttcgtg gaatccacct taaccggcgg
     2881 cttgtaggga acatcttcgg tactgttgat tgttttctta caggcgaata aacaaagcag
     2941 cgccagtatc aggatcgttg atccggggtg tagatttttt ttcatgggta ttcggggatc
     3001 tgatgaaata atactcaaac aagatagaag taaaaacctg catttgcaag ggttttcccc
     3061 gaaatcaatg taaaaaacac ggtcattgat gtttaaacat tcagccggaa acaaaggcgg
     3121 aaacgaccat cttaaatagg ggaaagcatt tcggaaacgg cccggctttc ccggataaat
     3181 aaatttccca tttggggact gcctgaacgg ttcctacaat ttgaagaata ttttttttct
     3241 gtatagccag aagcacaggc cccattccag aatgaaaaca gcaagggaaa ccatcacctc
     3301 aacgggtagc ggggacaagg caaacgggga aatgatccca ctgaccagcg tatgcatata
     3361 atcgtaaagc cagcggtcaa ccacaatctc gaaaaaaaca taaatgaaaa tggaattcat
     3421 gccgacgata agaaagaatt ttaaaaaccg cttatgcccg agcacatcaa tccatccata
     3481 cagacaggcg agtgccagca aacagtagcc accgctgacc agggtgaagg aactggtagc
     3541 aatgcgttta atgatgggcg taataaggca aaaatccaat aaatagccgc agaccagggc
     3601 cgcccctgcg aagatcagga tccggcggat ccggctttta ttgtccctga gttcaatcag
     3661 ccatttgccg gctaccgccc cccagatggt gtgcgcagca gtaggaatgc aattaatagc
     3721 cacccagccg cccggattga ttttattcat caggatcagg tccacgtagt ttccaaaatt
     3781 atgctgatcg gtaaacggct gatcaaaccc gggaaaatgc gaaaaacggt aaaggcattc
     3841 ggtcagcagc aacaagccca cgctgaaaag aatctggtag cccgttttcc agttaaaaat
     3901 aaaaaaagcg accagcatgg taaaagacag ctgggtgaga acatcccata gttcaaagga
     3961 taaatgatcg cctcttacgg agtaatccag cacgccccag aaaaatagcc agccacttct
     4021 tctcagtgct ttacctgccc gggatttcca gggaacgccc tgcgccttct gtttgtggat
     4081 cgaataggcc atggccaccc cggcgataaa catgaaaccc ggttgaatga gatcccagaa
     4141 atgcaacccg tgccagggat gatgaaaaaa ctggttgatc agcggttcca aaaatccgcc
     4201 ctttgcccct tctcccagat aaccatagag ccgcgtactt tccagcatga gcagcaccat
     4261 gatgaggccg cggtaaaaat ccagggagat caggcggccg cctgccggta aatttttttc
     4321 ggggagtttg taagcgggag aaatcatact gaccaaaagg atggttaaac aagataggaa
     4381 atttgggaga tttcattatg gtttcattac cagcaggagc ttacggggag acccgcttaa
     4441 gaaatatgcc tttaagcgtc cggaggaaag tttttaaaat aaatggcttg ccgtataatc
     4501 cggattattt atgtttacaa taacatttgc tttttgttta catctatgac aacgcccgcc
     4561 accggtctta tctgctggtc atttctgatc agtgcactgg tttgcagctg caatccaagg
     4621 cagcgtttgt tcaaaaaaat accttccgcg gtctccggca ttcatttcaa taatgagata
     4681 attgaaaatg attcgattaa cccgatcgat aaagaattcc tgtacaatgg cggaggggtt
     4741 gcagtcgggg atttcaataa tgatggcctc caggatctct attttaccgg cagcatggtc
     4801 tccaataaat tatacctgaa ccagggaaat ttcaggttcc gggatatcac gcgggcggcc
     4861 ggcgtcaccg gcgacggacg ctggtgcagc ggtgtttccg ttgtggatat taataatgac
     4921 gggctactgg atatttacgt ttgtaccacg ataaaaaatg acccggcgga aaggaccaat
     4981 ctgctctaca taaaccaggg aatggacaaa aaaggtattc ccatatttaa ggaaatggcg
     5041 gcggagtatg gcctcgcgga caccagttat tcggtgcagg cagctttttt cgactacgat
     5101 aacgatggcg acctggacat gtatctcgta accaccaaaa tggcgcaaag ggatgcggca
     5161 caatttgcca gcaatagcat aaaccgggat tcggccgatg tcgataaatt atttagaaac
     5221 gactgggatg cccgcctcgg ccatcccgta tttacagacg tttccaggca ggcaggaatt
     5281 acccaccagg gatatggatt gggcgtatcc attgtggata ttaacagaga cggatggaag
     5341 gatatttatg taaccaatga tttttacgga agcgatcaac tctatataaa taataagaac
     5401 ggcacttttt cagaccgggt gagcagttat tttaaacata cttcccagaa tgccatgggt
     5461 aatgatatcg ctgatatcaa caacgatggg ttggcagacg tactgacgct ggatatggac
     5521 ccgccgggca atttccggaa gaaaaaaaat atgagcgccg gcaattattc tatttatcag
     5581 catatggcga acgggaatta tatgatgcaa tacgtgcgca atacgcttca gctaaaccag
     5641 gggccgcagg taaccggaac cgattcgatc gggaacccgg tttttagcga tatcggtttt
     5701 tatgccggag ttgccgaaac agactggagc tggaacgctt cccttgcgga ttttgacaat
     5761 gacggcaacc gcgacatttt tatcaccaac ggatacccga aagatatcac cgaccatgat
     5821 tttgccgcgt tcagaagaca ggcatccgat ctgctgtcta aaaaaagcct gagcgctcaa
     5881 attcctcaaa tcaaaatacc aaactgtgta tttaaaaata aaggaaatct gcaatttgaa
     5941 aatatgacga taccctgggg actggaggaa ccttccttct ccaacggggc cgtttatgca
     6001 gacctggata atgacgggga cctggattac gtaatcaata acatcaatgg cgaagccttt
     6061 gtatatgaaa acagcacgat cacgggaaag aa
//
LOCUS       DASYCU010000585         5732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1286227, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000585 DASYCU010000000
VERSION     DASYCU010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286227"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..178
                     /locus_tag="VGM24_11810"
     CDS             26..178
                     /locus_tag="VGM24_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY4156112.1"
                     /translation="MDTCKPYDKAGAYAIQEWIGVTGIRAIHGDFYNVMGLPVSRVIQ
                     QLKTFS"
     gene            355..1635
                     /locus_tag="VGM24_11815"
     CDS             355..1635
                     /locus_tag="VGM24_11815"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEY4156113.1"
                     /translation="MTEKMLQFIWKYRHFNQQGLTLLSGEALTIEFPGEENKNQGPDF
                     IHARIRINNTCWVGSVELHLLSSGWDKHGHAKDENYRNVILHVVWMQDKSDPGRNIPQ
                     LELYQRVSRIMLDTYAGWMNQHAFVPCEKSLQSVARKNWDSWKQALLLIRLDRKLSSI
                     RDSLRMNHFHWEEQLWWMMAGNFGMTVNTAAFESVARSIPFNLLARHRQQRIQVEALL
                     FGQANLLEENFREAYPLMLKKEFNFLKRKYGLRKVFDQVRFLRMRPENFPGIRLSQLA
                     ALCMDGSNLFAWILSCKELPELRKKLDLSASDYWSSHYVFDKESRYKVKLLGIRTVEI
                     IIINSVIPLLYTYGMLYPDLPARQKALSWLQEIRAEQNTILSGWKQLGIKIKSAADSQ
                     ALLELRKEFCRQKKCLDCDIGKFLLLPQDKQAGG"
     gene            complement(1638..2075)
                     /locus_tag="VGM24_11820"
     CDS             complement(1638..2075)
                     /locus_tag="VGM24_11820"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY4156114.1"
                     /translation="MEKYMTSTVIYVGELRTVARHLSSGSQIETDAPTDNQGKGEKFS
                     PTDLLATSLGSCMLTIMGMKARDLGVDLNNTRIEIQKQMKADPRRIGGINLTFHFSES
                     LRVTEKQRLILERAGDTCPVRYSIHPDIEVLVVYNWPAVIAAQ"
     gene            2100..2909
                     /locus_tag="VGM24_11825"
     CDS             2100..2909
                     /locus_tag="VGM24_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156115.1"
                     /translation="MALKKFLFLVCFSLPALSLLAQQSPDVNYNSSRNSAKKERINRL
                     MRMEEEGDLIFDHHSVFGLRIATDGYGIFYERGKFKSVRKTSLLQFELNEKKDPKDHK
                     VAVGGFNGYTLNSIAIGRMNNFYQFKAAYGQQYLIGGKGNKNGVAVTGLYSGGVSVGA
                     LKPYILHVFKSQGDGTLFESRYPEILDSGYLVNDAAGLGSGWNKLSIKPGLNAKAAIR
                     FDYGRFNQTVTAIEAGATVEYYFSKIPLLALVKQKQLFFNAYISIMFGRRK"
     gene            2926..3861
                     /gene="lipA"
                     /locus_tag="VGM24_11830"
     CDS             2926..3861
                     /gene="lipA"
                     /locus_tag="VGM24_11830"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY4156116.1"
                     /translation="MQELPVLPNINPSEPFIKKPGWLRVKLPIGENYRQVRNLVDQHK
                     LHTICESGNCPNMGECWGAGTATFMILGNICTRSCGFCAVATGRPLAVDWDEPQRVAE
                     AIHLMKVKHAVITSVDRDEIPDGGSIIWQNTIRAVKALNPETTLETLIPDFKAEEENI
                     QRIIEVAPEVVSHNIETVERLTKQVRIQAKYWRSMQTLNFLKKGGMRTKSGIMLGLGE
                     TKEEVIRTIKDLAANGVDVITIGQYLQPTKKHLPVQRFVHPDEFAELREIGYQLGLDY
                     VESGPLVRSSYHSEKHVVAGFGRDQWNKQRLMVNR"
     gene            complement(4104..5090)
                     /locus_tag="VGM24_11835"
     CDS             complement(4104..5090)
                     /locus_tag="VGM24_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY4156117.1"
                     /translation="MAKATSTPASLEPLAIGIDIGGTGTKFGIVDRNGNVLFSGEIST
                     KKHRQVETFIDELHESLSALIQNVGGPGRIRGIGVGAPNGNYYTGTVEYAPNLPWKGI
                     IPLTQLIQDKFQLPGILTNDANAAAIGEMMYGAAKGMKDFIMITLGTGVGSGIVANGH
                     LILGHDGFAGELGHTIIIPDGRFHEGTGKYGSLESYASATGVTLTAKEVLEKTNAESI
                     LRQIPMEEIDSKKVYDAAILGDVLAKEIFDYTGKILGLALANFVMFSSPEAIVLFGGL
                     TKAGDLILKPTREHMEANLIQVFQNRVKILVSHLRESDAAILGASALVWEMT"
     gene            complement(5108..>5732)
                     /locus_tag="VGM24_11840"
     CDS             complement(5108..>5732)
                     /locus_tag="VGM24_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4156118.1"
                     /translation="SKYITLYWGSLMIGRWTGAIGVFNLSATARKIATILVPFIAFAF
                     VLFINHTYGNDITDLLPYAAWVAVAIFAFFFGQQKPVKTLLTVTLLGSAAMLLGVSTK
                     GLFSVYCFMAGGLFCSVMWPSIFALAVAGLGKYTSQASAFLIMMILGGAVIPPFQGSI
                     GDTTNNMHISYLVAAGCFAALALLAVRMRTILKSQGLNFDEQIGSSH"
BASE COUNT         1475 a         1445 c         1418 g         1394 t
ORIGIN      
        1 ggatatttcc ggaatcgaat attatatcga tacctgcaaa ccgtatgata aggcgggcgc
       61 gtatgccatc caggaatgga tcggggtcac cggcatccgg gcgatccacg gcgattttta
      121 caatgtgatg ggattgccgg tcagtcgcgt gatccagcag ctaaagacat tttcctgatc
      181 gtcaaagtct gttacacccc gatttttata gtttttacct aaattttttt cgtttctgcc
      241 cttgttaaat aaagggccgg aatttagatt tgtttccatc cgttattcat gtagcggata
      301 gataaagaac gggtatggat tccttccggt caccggaatc ctcaagcagc aggaatgaca
      361 gaaaaaatgc tccagttcat ctggaaatac cggcatttca atcagcaggg tttgacgctc
      421 ttgtccgggg aagcattaac catcgaattc ccgggggaag aaaataaaaa tcaggggccc
      481 gattttattc atgcccggat ccggattaat aatacctgct gggttggaag cgtggaattg
      541 cacctgcttt cctccggctg ggataaacac ggccatgcaa aggatgagaa ttaccggaat
      601 gttattttgc atgtggtctg gatgcaggat aaatccgatc cgggccggaa cattccccag
      661 ctggagttat accagcgagt ttcccgtatc atgctggaca cttatgcggg ctggatgaac
      721 cagcatgcgt tcgtgccctg cgaaaaatcc ctgcaatccg tcgcacggaa gaactgggat
      781 tcctggaaac aggctttatt gctgatccgg ctcgaccgga aattgtcgtc gataagagat
      841 tccctccgga tgaaccattt tcactgggaa gaacaactct ggtggatgat ggcgggaaat
      901 ttcgggatga cggtaaatac ggcggctttt gaatcggttg cccggagtat tccgttcaac
      961 ctgctggcca ggcaccggca gcaacgcatc caggtggaag ccctgttatt tggccaggcc
     1021 aatctgctgg aagaaaattt cagggaagcg tatcccctga tgctgaaaaa ggaatttaat
     1081 tttcttaaaa gaaagtatgg cctgagaaaa gtatttgacc aggtccggtt cctgagaatg
     1141 aggccggaga attttcccgg catccggctt tcccaactgg ccgctctctg tatggatggc
     1201 tcaaacctgt ttgcctggat tctctcctgt aaagagcttc cggaactcag gaaaaaattg
     1261 gatctgtccg ccagtgatta ctggtcttct cattatgtgt tcgataagga gtcccgttac
     1321 aaagtaaagc tgctgggaat aagaaccgtt gaaatcatta tcatcaacag tgtgatccct
     1381 ttgttatata cttatggaat gctgtacccc gacctgccgg cccggcaaaa agcgctgtcc
     1441 tggttgcagg aaatccgggc agaacagaat accatcctct ccggctggaa gcagcttggg
     1501 atcaaaataa aatccgctgc ggattcgcag gcgctgctcg aattgagaaa agaattctgc
     1561 cggcaaaaaa aatgtctgga ctgcgatatc ggtaaatttc ttcttttacc gcaggataaa
     1621 caggcaggcg gataacccta ttgcgcggca ataacggcag gccagttata gacgaccaat
     1681 acttcaatat ccggatggat gctgtatcgt accgggcacg tatcgcctgc tctttccagg
     1741 atcagccgtt gtttttcggt aacccgcagg gactcggaaa aatgaaaggt taaatttatg
     1801 cccccgatac gccggggatc cgctttcatc tgtttttgaa tttctatgcg ggtattgttc
     1861 aggtctacac ccagatcacg ggctttcatt cccatgatcg tcagcataca acttcccaaa
     1921 gaggtggcca gaagatcagt gggagagaac ttttccccct ttccctggtt gtcggtcggg
     1981 gcgtcggttt ctatctggct gcccgaagaa agatgacggg caaccgttct gagttctcct
     2041 acgtaaataa cagtggaggt catgtatttt tccataaatt tacagcagta atctcaatca
     2101 tggcgttaaa aaagttcctg ttcctggttt gtttttcctt acccgccctt tcccttcttg
     2161 cccagcaatc accggatgtc aattacaatt caagccgtaa ttcggccaaa aaagagcgga
     2221 tcaaccggct catgagaatg gaagaagaag gagacctgat cttcgatcac cacagcgttt
     2281 ttggattgcg catcgccacg gacgggtatg gtatctttta tgaacggggc aagttcaaaa
     2341 gcgtccggaa gacctccctg ctccagtttg aattaaatga aaaaaaggac ccgaaagacc
     2401 acaaagtggc ggttggcggg tttaacgggt ataccctgaa ttccatcgcc atcgggcgga
     2461 tgaataattt ttaccagttc aaggcggcct acggtcagca atatctgatc ggaggaaaag
     2521 gaaataagaa cggggttgcc gtaaccggtt tatacagcgg cggcgtgtcg gtcggcgccc
     2581 tcaaacccta tattttacat gtatttaaat cccagggaga cggcacttta ttcgaaagcc
     2641 gttatccgga gatcctggac agcggatacc tggtgaacga tgcggccggc ctgggatccg
     2701 gatggaataa attaagtata aaaccgggtt taaatgccaa agcggcaatc cgcttcgact
     2761 atggccggtt caaccagacc gttacggcca ttgaagccgg agccaccgta gagtattatt
     2821 tcagcaaaat tcccctcctg gccctggtaa aacagaaaca gctgtttttc aatgcctata
     2881 tcagcattat gttcggccgg cgaaagtgac tatttttgca tgatgatgca agaactccct
     2941 gtacttccca atataaatcc ttcggaaccc tttattaaaa agccgggctg gctccgggta
     3001 aaacttccca tcggtgaaaa ttacaggcag gtccgcaacc tggtggacca gcacaaactc
     3061 cacacgatct gtgaaagcgg caactgcccg aatatgggag agtgctgggg agccggtacg
     3121 gccacgttca tgatcctcgg taacatctgt acacggagct gtggcttctg tgccgttgcc
     3181 accggccgcc ccctggccgt tgactgggat gaaccgcagc gggtcgccga agccatacac
     3241 ctgatgaaag taaaacatgc cgtgatcacc tccgtcgacc gggatgagat cccggatgga
     3301 ggcagcatca tctggcagaa taccatccgg gcggtaaaag ccctgaatcc ggagactacg
     3361 ctcgaaacct taattccgga ttttaaggcg gaagaagaaa atattcagcg catcatagag
     3421 gtggcgcccg aggtggtgtc tcacaatatt gaaacggtgg agcgccttac caaacaggtg
     3481 cgtatccagg caaaatactg gagaagcatg caaacgctga atttcctgaa gaaaggaggc
     3541 atgcgcacga agagcggcat catgctggga cttggggaaa ccaaagaaga agtgatccgg
     3601 acgataaagg acctggctgc caatggagtg gatgtgatta ccatcggcca atatcttcag
     3661 cccaccaaaa aacatttgcc cgttcagcgg tttgtccatc ccgatgaatt tgccgaactc
     3721 cgggaaatcg gctatcaact cgggctcgat tatgtagaaa gcggtcccct cgtgcgctct
     3781 tcctatcaca gcgaaaaaca tgtggttgcc ggcttcggcc gggatcagtg gaataagcaa
     3841 agactgatgg tcaatcggta aagtctttat ccgggcagct tctgcagaga tagatgggct
     3901 ggcatgattt ttaaaattga taccgtaacg gctttgccgt ctttttaaaa atcaaatacc
     3961 ggtttcatga aacgagtcct tttcatcctg ctttgtatcg cgtttctttt ttcctgcgcc
     4021 ccggcacgcc atgctccgcg tccaccacat ccgccggttc ctccgggagc ccctgttccg
     4081 ccggcgccat agggactacg cgttcaggtc atttcccaaa ccagggcgct ggctcccagg
     4141 atcgccgcgt ccgattcccg gagatgactt accagtatct ttacccggtt ctgaaatacc
     4201 tggatcaggt tggcttccat atgttcgcgg gtcggcttga gaatgagatc acccgccttg
     4261 gttaaaccgc cgaatagtac tatggcttcc ggactggaaa acatcacgaa attggccagg
     4321 gccagaccca ggatctttcc ggtataatcg aagatctctt ttgccagcac atccccgaga
     4381 atcgcggcat catatacttt tttcgaatcg atctcttcca tgggaatctg ccggagaatg
     4441 ctttccgcat tggtcttttc caaaacctct ttcgcggtaa gcgtcacgcc ggtagccgag
     4501 gcgtagcttt ccagggatcc atatttaccc gttccctcat gaaatcttcc atccggaatg
     4561 ataatagtat gtccgagctc cccggcaaac ccgtcatgac ccaatatcag gtgcccgttc
     4621 gctacgatgc cgctgccgac gccggtaccc agcgtgatca taataaaatc cttcatccct
     4681 ttggccgcac cgtacatcat ttccccgata gcggccgcat tggcatcatt ggtcaggata
     4741 cccggcaact gaaacttatc ctggatgagc tgggttaatg gaataatgcc tttccaggga
     4801 aggtttggcg catattcaac ggtgccggta taataattcc cattgggcgc accgacgccg
     4861 atcccccgga tcctgcccgg tccgcctaca ttctgtatca gcgctgaaag cgactcatgc
     4921 agttcgtcaa taaacgtttc gacctgtctg tgcttttttg tggatatctc tccgctgaat
     4981 aagacatttc cattccggtc tacaatgccg aacttggtac ccgttcctcc gatatcaatt
     5041 ccaattgcca aaggttccag tgaagccggc gtagaagttg cttttgccat tctcttttat
     5101 tcaatattta atgggaactc ccgatttgct cgtcaaaatt aagtccctgg gatttaagaa
     5161 tggtccgcat tctgaccgcc agtaaggcca acgcagcaaa acagccagca gcaaccaggt
     5221 aggaaatatg catattattc gttgtatctc caatggaacc ctggaatggc ggaatcacgg
     5281 cgcctcctaa aatcatcata ataaggaaag ccgaagcctg gctggtatat ttacctaaac
     5341 cggccacagc gagcgcaaaa atactgggcc acatgacgga acagaaaagt ccgccggcca
     5401 taaaacagta taccgaaaac agccccttgg tggacactcc gagcagcatc gccgccgatc
     5461 ccagcagggt caccgtcagt aaggttttca ccggtttttg ctggccgaag aaaaaggcaa
     5521 aaatagccac cgccacccag gccgcatacg gaagcaggtc tgtgatatca ttcccgtagg
     5581 tgtggttaat aaaaaggaca aaggcaaagg caataaatgg aacaaggatc gtagcgatct
     5641 ttcttgccgt tgcggaaaga ttgaaaacgc cgatcgcgcc ggtccacctt ccaatcatca
     5701 ggcttcccca gtacagggta atatatttgg aa
//
LOCUS       DASYCU010000586         3613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1578664, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000586 DASYCU010000000
VERSION     DASYCU010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578664"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VGM24_11845"
     CDS             <1..726
                     /locus_tag="VGM24_11845"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4156119.1"
                     /translation="FTYGGYQQTINFGAEVSNPSRQIPRAIFFGISIIILLYLSINYA
                     YVKVIGLNGLRTSRGIASVMAAHVFGEAAGKVLSILLFLSVLAYVNVLLLSNPRVMYA
                     MSEDGVIPAVFGKKFRSNGVLFTSLSAFTAVCLLVVFWAKEFDEILSFTIFLDCFGMA
                     ASAGTIFILRKREVQPEGSKIYRMKIYPLMPLVFIAAYVYVAISIFIDRPATALTALG
                     VLALFMIIYFLGGKRGMRKADFR"
     gene            879..3404
                     /locus_tag="VGM24_11850"
     CDS             879..3404
                     /locus_tag="VGM24_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY4156120.1"
                     /translation="MRKRLLTLLCVSLSLLCWNTDYAQVAGNPRLKTPNQTALEVDKS
                     PTRLSPELKKLAAQAGGQNQRRLNANIKPIPPNDALDKYMQIQGNGVVIDITVKGNIS
                     SAKTELQKKGLQITGAFGRVISGIFPIASLAQLETLSSVQYARPAFKPIHPGRSPGHP
                     GSGNKPGKQVSPVISQGDTAQRSEIARKKYNVNGKGVKVGILSDSYNNLGTAGKGVKQ
                     GELPGPGNPFHFNKAVEVLQDLDSGGTDEGRAMLEIVHDVAPASELAFHTTSLGQANF
                     AQGIQDLANRGCQVIADDALYFAEPFFQDGIIAQSVDLAKKKGVAYFSAAGNESVRSY
                     ENDYHPSDVEFLGPGVGTAHNFSAPSDPPVYAQPIYIPPGGSFICSFQWDQSSFSASG
                     VGASSDLDIYLLDINGNVVAGAASDNIASGDPIEVFGFQNESQDPTFFLVILKYAGPD
                     PNHIKYVSFNDALFYLTDPPIPGILAPTLYGHAKAAGAIATAAAFYLQTPAYGLNVPV
                     VEPYSSVGGVANFFDVNGNRIPPLIRKKPDITAPDGGNTSFFDPFGGGDISEDADTYP
                     NFFGTSAAAPHASGVAALMIEAQKLKTITPDQIRGILSTHTSDIDDPATSGFDKGFDF
                     ASGYGLIRADKAVGTVKFPNQYFQNLKLKAACSDNPAVIRNWTISNPNPFEVEVQWFL
                     AGTSQRGSLLAAPGNTSFSTSTNGNYFVSNILIIDWQDNFGFTRVDAGFSSGARCGKD
                     AVDASDGQALEAYSKEAAQQKTVAEVYPNPATDNFNLYLSLDNDQPVEIELSSLDGKR
                     LYSSRTMKSNGVISIPASSYRPGMYIIKLKQGAFFKTFKVIKQ"
BASE COUNT          858 a          893 c          933 g          929 t
ORIGIN      
        1 tttacctatg gcggatatca gcaaaccatt aatttcggag cggaagtgtc caatcccagc
       61 cggcagatac caagggctat ttttttcggg atatccatta tcattttatt gtacctcagc
      121 attaactacg catacgtaaa agtgatcggg ctcaacgggc tccggacttc caggggcatc
      181 gcctccgtaa tggctgccca tgtatttggc gaagcggcgg ggaaagtgct ttccatttta
      241 ttattcctct ccgtgctcgc ctacgtgaat gtattgctgc tgagcaatcc gcgggtgatg
      301 tacgcgatga gcgaagacgg agttatcccg gctgtatttg ggaaaaaatt caggtccaac
      361 ggcgtcctgt tcacttcttt atctgctttt acggcagtct gcctgctggt ggtgttctgg
      421 gcgaaggagt ttgatgaaat actcagcttt accatcttcc tcgattgttt cgggatggcc
      481 gcctccgccg gcaccatttt catattgagg aagagagagg ttcaacccga aggaagtaaa
      541 atttaccgga tgaaaatata tccgctgatg ccgctggttt ttattgcggc ctacgtgtac
      601 gttgcgatca gtatttttat tgaccggccg gcgacggccc tcactgcact gggcgtgctg
      661 gctcttttca tgatcattta ttttcttgga ggaaaacgcg gaatgcggaa ggctgatttc
      721 cgatagtccc tgcgcatacc ggctgccggt cagctccgct gatctttgat cccgccgtcc
      781 aacgcccgcg aggaaattta tgggattctt ggaaaaatag gttaaattga ttaggagaaa
      841 acgcccccct attatttcac ctcttcaacc ttcagcttat gagaaaacgt ttacttaccc
      901 tcctttgcgt ctccctgtct ttattatgct ggaatacaga ttatgcgcag gtagccggca
      961 atccccggtt aaagactcca aaccagacag ccctggaagt ggacaagtcc ccgaccaggt
     1021 taagtcctga actaaaaaag ctcgctgctc aggccggcgg acagaaccag cggcgcctga
     1081 acgccaatat caaacccatt ccaccgaatg acgcgctcga taaatacatg cagatccagg
     1141 gaaacggcgt ggtgatcgat atcacggtga aggggaatat cagttcagcc aaaacggaat
     1201 tgcaaaaaaa aggattgcag ataaccggtg cttttgggag agtgatctcg ggtatctttc
     1261 ccattgcttc cctcgcccag ctggaaacgc tctcctccgt tcagtatgcc cggccggcat
     1321 ttaaacctat acatccgggc aggtcgcccg ggcatccggg aagcggaaat aaaccgggaa
     1381 aacaggtaag ccctgtcatc agccagggcg atacggcaca gcggtccgaa atagccagga
     1441 aaaaatataa tgtgaatggg aagggagtta aagtcggtat cctctcggat agttacaata
     1501 acctgggaac tgccggtaaa ggggttaagc agggagaact tccggggccc ggaaacccct
     1561 ttcattttaa taaggccgtg gaagtgctgc aggatctgga cagcggcggt acggatgaag
     1621 gccgcgccat gctggagatt gtgcatgatg tggctccggc ttctgagctg gcatttcata
     1681 ccaccagcct gggccaggcg aattttgctc aggggataca ggatctggcc aaccggggct
     1741 gccaggtgat tgccgatgat gccttgtatt ttgccgagcc ttttttccag gatgggatca
     1801 ttgcccagtc ggtcgacctg gcaaagaaaa aaggagttgc ttatttttcc gctgccggga
     1861 atgaaagtgt ccggtcatat gaaaatgatt atcatccgtc ggatgtggaa tttttgggac
     1921 cgggcgtcgg tacggcccat aatttcagcg cgccttcgga tccgccggtt tatgcgcagc
     1981 ccatttatat tccgccgggc ggctcattca tttgttcctt tcaatgggac cagtcttcct
     2041 ttagcgccag tggcgtgggc gcatcttccg acctggatat ttacttgctg gatattaatg
     2101 gaaatgtggt ggccggagca gcgagcgaca atattgccag cggcgatccc atcgaagttt
     2161 tcgggtttca gaacgaatcc caggatccca cttttttcct ggtgatttta aaatatgcgg
     2221 gccctgatcc caaccatatt aaatatgttt cttttaatga tgctttattc tatctcaccg
     2281 acccgccgat cccgggcatt ctggcgccaa ccctttatgg acatgctaaa gcggcgggag
     2341 ccattgccac ggcagctgct ttttacctgc agacgcccgc ctatggcctg aacgttccgg
     2401 tcgtggaacc gtattcatcc gtcggtggtg ttgccaattt ttttgatgtg aatggtaacc
     2461 gcatccctcc gctgatccgg aagaaaccgg atatcacggc gcccgatggc ggaaacactt
     2521 ctttcttcga tccctttggc ggcggcgata tttcggaaga cgccgatacc tatcccaatt
     2581 ttttcggcac ttcggcagcg gcgccgcatg cctccggcgt ggcggctctc atgatcgaag
     2641 cgcagaaatt gaagacgatt accccggatc agatccgggg gatattgtcc actcatactt
     2701 cggatatcga cgatccggct accagcggat ttgacaaagg atttgatttc gcttccggat
     2761 acggacttat ccgggccgac aaggcagtgg gcacagtgaa atttcccaac cagtattttc
     2821 aaaacctgaa attgaaggcc gcctgttcgg ataacccggc ggttattcgt aactggacca
     2881 tcagcaatcc aaatcctttt gaagtggaag tgcagtggtt cctggccggc acatcgcaaa
     2941 gaggcagcct gctcgcggct ccgggcaata cctctttctc gaccagtacg aatggaaatt
     3001 attttgtgtc caatattttg atcatcgact ggcaggataa ttttggattt acccgcgtgg
     3061 acgccggatt cagcagcggc gcccgctgcg gaaaggatgc agttgatgct tcggacggac
     3121 aggcgctgga agcgtacagc aaagaagcgg ctcaacaaaa aacggtggca gaagtatatc
     3181 caaatccggc aacggataat ttcaacctct atctctccct ggataatgat cagccggtgg
     3241 aaatagaact gtccagcctg gatggtaaac ggttatatag cagccggacg atgaagtcaa
     3301 acggagtgat cagtattcct gcatcttcct acaggccggg aatgtatatt atcaaactga
     3361 aacaaggcgc ttttttcaaa acgtttaaag taataaaaca gtaaattttt tccggtatgg
     3421 gataaaagcg gggctgtcct ttggtgcagc cccgcttttt attttgcccg gggcggatat
     3481 gatttttggt ctgtaaattc taccacatcg tggtattttt tccgctccgg tttgttccta
     3541 ttttgcttct tcaatatggg aattccgaaa cccatgcgtt ttgcgaagat tcgaatcaaa
     3601 taaaaatgtg cgt
//
LOCUS       DASYCU010000587         4456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5054943, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000587 DASYCU010000000
VERSION     DASYCU010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5054943"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1166)
                     /locus_tag="VGM24_11855"
     CDS             complement(192..1166)
                     /locus_tag="VGM24_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY4156121.1"
                     /translation="MDIPPKILVIGACGQIGTELTLALRQKYGGDSVVAADLKRESDL
                     LKGGGPFLPLDIMDKKILQVQVKRHGINTIYLLAAMLSASGEQQPELAWKLNMESLLN
                     TLEVAREEKINRIFWPSSIAVFGPDAPKRDCPQNAWSTPATVYGISKLSGEQWCHYYY
                     RRYGVDIRSIRYPGLISYKTLPGGGTTDYAIEIFHAAIRAESYSCFLRQDTRLPMMYM
                     DDAVRGTIELMEAPASRLTVRTAYNLAGISFTPAEVAAAISRLIPDFAIEYHPDYRQS
                     IAESWPESIDDLTARNDWGWKPEYNLHRMTGEMIRHIRPELAMSTKSK"
     gene            complement(1231..1686)
                     /locus_tag="VGM24_11860"
     CDS             complement(1231..1686)
                     /locus_tag="VGM24_11860"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY4156122.1"
                     /translation="MKKLFTLLPALLFVFAGLAQAHLRPYTWSYAAKKISGNSYELHF
                     IIDVSSPWHTYSQFTPDGGPVPTQFSFTKNPLYTLEGKVKENGKLLKKHESVFGVDVK
                     YFNGKVDFVQLVKVKGNAKTNFSGSVEFMVCNDQQCLPPATEKFSIPLN"
     gene            complement(1813..2790)
                     /locus_tag="VGM24_11865"
     CDS             complement(1813..2790)
                     /locus_tag="VGM24_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY4156123.1"
                     /translation="MKLISYLQDRQEYFGLIRDGQVYSLQELDERLPQIIDPFLLQWN
                     LLLPLVRDAEQRLIADPRLQASGLPLREVRLLPPVPRPGSFRDGYAFRQHVETARRNR
                     GLPMIPEFDRFPVFYFTNHRSVRGPGDVPCMPDHFQQLDYELEAAILISKRGRNIPAQ
                     KADEYIGGMMIMNDFSARGLQMEEMKLNLGPVKGKDFAISAGPWLVTPDELESFETAG
                     ARGHTGKAWNLEMTCSVNGVELSRGNLSDMQWTFAEIVERASYGADLYPGDLIGSGTV
                     GTGCLLELNGTATRQNPKSVPQWLKAGDRIEMKISGLGVLDNTIVQEMV"
     gene            complement(2799..3929)
                     /gene="hppD"
                     /locus_tag="VGM24_11870"
     CDS             complement(2799..3929)
                     /gene="hppD"
                     /locus_tag="VGM24_11870"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY4156124.1"
                     /translation="MERKNSVLSSSPEEKDFLPLYGTDYIEFYVGNAKQAAHYYISAF
                     GFQGIAYAGPETGVRDRASYVLRQNKLTFVLSTSLRAGTAMADHVNKHGDGVKAIALL
                     VDDAETAWRETTRRGAVSYLEPEVLTDDAGRIGISGICLYGETVHLFIDRAGYSGLFM
                     PGYRKWEPGLETKPTGLLYVDHCVGNVGWNQMNKWVSFYENVMGFKNILTFDDQDIST
                     EYSALMSKVMSNGNGFVKFPINEPAEGKNRSQVEEYLDFYAGEGVQHVALATGDIIRT
                     VSDLMKRGVAFLKVPEVYYEGLEERVGKLEEDLRPLRKLGILVDRDPEGYLLQIFSKP
                     VEDRPTIFFEIIQRKGAKSFGKGNFKALFEAIEKEQAARGNL"
     gene            complement(4088..>4456)
                     /locus_tag="VGM24_11875"
     CDS             complement(4088..>4456)
                     /locus_tag="VGM24_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156125.1"
                     /translation="KLDGLLSLLMKDFEEKNSIPLGLFHDRYGEFFGEDLERLEEILF
                     EDGCIRGTVGPEGLSIEISPKGMQFMSKGGYSSEIREEMISHEDALRAQKIAKKWNKV
                     VLAGFVVAMAFCFYLRHRSL"
BASE COUNT         1072 a         1214 c         1054 g         1116 t
ORIGIN      
        1 caaaagtaat aaatccgggt ttattacaga gaaaaatatc gaaggcccgg cggtataggc
       61 cacagccata aattttctga ttcagcatcg gatccattcc cgattttcct atcggggcca
      121 tccggaaaaa ttattgaaaa acggatctga atttttattc tccggcccgg gcacccggat
      181 tgccgccagc actatttgct tttggtagac atggccagtt ccggtctgat gtggcggatc
      241 atttctccgg tcatccggtg caggttatat tcaggcttcc agccccaatc gttgcgggcc
      301 gtcagatcat caatcgattc aggccaggac tccgcgatgg actgccggta atccggatga
      361 tattcaatgg cgaaatccgg gatcagcctg ctgatcgcgg cagccacttc agcaggcgta
      421 aaactgatgc cggcgagatt atatgccgtg cggacagtca gccgggaggc cggtgcttcc
      481 atgagctcaa tggttccccg gacggcatcg tccatataca tcatcggcag tctggtatcc
      541 tgccggagaa aacaggaata ggattctgcc cggatggccg catgaaaaat ttcaatggca
      601 tagtcggtag ttcctcctcc gggcagggtt ttataactga tcagtcccgg ataccgtata
      661 cttcttatat ccaccccgta ccgcctgtaa taataatggc accattgttc cccacttaat
      721 ttactgatgc cgtaaacggt ggccggcgtc gaccaggcgt tctggggaca atcccgtttt
      781 ggtgcgtccg gaccaaaaac agcgatgctg ctgggccaga atatccggtt aattttttct
      841 tcccgggcaa cttccagcgt gttgagcagg ctttccatat tcagtttcca ggcgagttcc
      901 ggttgctgct ccccggaagc agacaacatg gccgcgagca aataaatcgt gttgatgccg
      961 tgccgcttca cctgcacctg cagaatcttt ttatccatga tatccagcgg aagaaaaggg
     1021 ccgccccctt tcaacaggtc cgattccctt ttcaggtctg cagcaactac cgagtcccca
     1081 ccgtatttct gacgcagggc gagggtaagt tcagtgccga tctgcccgca ggcaccaatt
     1141 accaatattt tcggaggaat gtccatagca gctaaagatt attcagcaag acgatgcctg
     1201 cccgcttgtt taaagcggaa tgcgtttcct ttaattcagc ggaatcgaga acttttcagt
     1261 tgccggggga agacactgct gatcattaca aaccataaac tccaccgaac cggagaagtt
     1321 ggtctttgca tttcccttta ctttcaccag ctgcacaaaa tccactttac cattaaaata
     1381 tttaacgtcg acgccaaaaa cactttcatg ctttttgagc agcttgccgt tttcctttac
     1441 tttcccctcc agcgtataca aaggattttt tgtaaaactg aattgggtgg ggaccggccc
     1501 gccatcgggg gtgaactggg aataggtatg ccagggactg ctgacgtcga tgataaaatg
     1561 cagttcatag gaattcccgg aaattttctt cgcggcatag ctccaggtat aaggcctgag
     1621 atgggcctgg gccaatccgg caaaaacaaa taaaagggcg ggcaggaggg taaacaattt
     1681 tttcatccgt tatcttattt tttgtgagtg catgaataac catgcaaagc taattattgt
     1741 aaaagggagt ttattccatt ctcccgttaa aacggtccat cgatcctggt gaacggtaag
     1801 catttgttaa aatcaaacca tctcctgcac aatggtattg tccagaacgc ccaggccgct
     1861 gatcttcatc tcgatccggt ctcctgcttt cagccattgc ggaacagatt taggattttg
     1921 ccgcgtagcc gtcccgttca gttcaagcag gcaaccggtt ccgaccgtac cgctgccgat
     1981 caggtctccg gggtacagat cggccccata ggaagcccgt tcgacgatct cggcaaatgt
     2041 ccactgcata tcggagaggt tgccccggct gagttccacg ccattgacac tgcaggtcat
     2101 ttccaggttc catgcttttc cggtatgccc tcgggcacct gcggtttcaa aagattcaag
     2161 ttcgtccggg gtcaccagcc agggaccggc cgagatggca aaatctttcc ctttgaccgg
     2221 ccccagatta agtttcattt cctccatctg caaacctctt gcactgaaat cgttcatgat
     2281 catcattccc ccgatatatt catccgcctt ttgcgcgggg atattccttc cgcgtttgga
     2341 aatcagtata gccgcctcca gttcatagtc gagttgctga aaatgatccg gcatgcaggg
     2401 cacatctccc ggtccacgga cagaccggtg attagtgaaa tagaaaaccg ggaaccggtc
     2461 aaattcgggg atcatcggaa ggccccggtt tctcctcgct gtttccacat gctgccggaa
     2521 agcgtacccg tcgcgaaaag agcccggcct cggtaccggc ggcagaaggc ggacctcccg
     2581 caggggtagc ccggaagcct gcaaacgggg atcggcaatc aggcgctgct ctgcgtcccg
     2641 gaccaggggc aacagcaggt tccattgaag caaaaaggga tcaatgatct gcggcaggcg
     2701 ttcatccaat tcctgcaatg aataaacctg tccgtcccgg ataagtccga aatattcctg
     2761 ccggtcctgt aaatatgaaa taagtttcat tacgatggtc acaaattacc gcgcgccgcc
     2821 tgctcttttt caatggcttc gaacaaggct ttaaaatttc ccttaccgaa gcttttcgct
     2881 ccctttcgct ggatgatttc aaaaaatatg gtgggtctgt cctccaccgg tttgctgaag
     2941 atctgcagca ggtatccttc cggatcacgg tcaaccagga tgcccagctt cctaagtggt
     3001 cgaaggtctt cttccagttt tccaacccgt tcttccagac cctcataata aacctccggg
     3061 accttcagga acgccacgcc gcgcttcatc agatcactca ccgtccggat gatatccccg
     3121 gtggcaaggg ccacatgctg caccccttcg ccggcataga aatccaggta ttcctccacc
     3181 tgtgatctgt tttttccttc cgcgggttcg ttaatgggga attttacgaa accatttcca
     3241 ttgctcatca ctttgctcat gagcgctgaa tattcggttg aaatatcctg atcatcaaac
     3301 gtaaggatat tcttaaaccc catcacgttt tcataaaaag aaacccattt attcatctgg
     3361 ttccatccca cattgccgac acaatggtcc acgtacagca ggccggtggg tttggtctcc
     3421 agaccaggtt cccattttct gtagccgggc atgaacaaac cggaatagcc ggcccggtca
     3481 ataaacaggt gcacggtttc cccatagaga cagataccgc tgattccgat cctgccggca
     3541 tcatccgtca ggacctccgg ttccagataa ctgaccgcac cgcgccgggt ggtttcccgc
     3601 caggcggttt ccgcatcatc cacgagcagg gcaatggcct tgaccccgtc tccgtgctta
     3661 tttacgtggt ccgccatagc cgttcctgcg cgtagggaag tgctgagaac aaaagtcagt
     3721 ttgttttgcc ttagcacata gctggcccgg tctcttacac cggtctctgg accggcgtag
     3781 gcaattccct gaaatccaaa agccgatata taataatgcg ccgcctgttt ggcatttccc
     3841 acataaaatt caatatagtc cgtgccgtat aaggggagaa aatccttttc ctccggagat
     3901 gaagaaagga cgctgttttt gcgttccata aaaaataaat gaatggggtt gagaaagaaa
     3961 atacccggcc tggccgtgga aagatttatc gcgatgcgga agccagggtc tccagaagaa
     4021 agacataatc gccgcgctgg tcgggaaaga ttttatatgg atagtccggc ctcattcgtg
     4081 tatccgatca aagagatctg tggcgaaggt aaaaacaaaa agccatcgca acaacaaagc
     4141 ccgccagaac caccttattc catttttttg ctattttctg ggcccgtaag gcatcttcat
     4201 ggctgatcat ttcctccctg atctcactgg aataaccccc tttggacatg aactgcatgc
     4261 ccttgggact gatttctatg gagaggcctt ccggacccac cgttccccgg atacagccgt
     4321 cctcaaaaag gatttcttcc aggcgttcca ggtcttcccc aaaaaattct ccgtaccggt
     4381 cgtggaacag acccagcgga atgctgttct tttcttcgaa atccttcatc agtaaggaaa
     4441 gtaagccatc cagctt
//
LOCUS       DASYCU010000588         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1437772, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000588 DASYCU010000000
VERSION     DASYCU010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437772"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VGM24_11880"
     CDS             complement(<1..585)
                     /locus_tag="VGM24_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY4156126.1"
                     /translation="MKILVCISKTPDTTAKIAFTDNNRKFAAEGVQWIINPYDEWYAL
                     VRAIELKEKDPGLVLHLITVGGADADAILRKALALGGDEAIRVNADSQDSFFIASQIA
                     AVAAGAGYDLIFTGKETIDYNGSSVGGMIAGILDLPYVSLAAKFDLAGNTATVVREVE
                     AGEETEEVSLPLVVSCQKGMAEQRIPNMKGIMGAR"
     gene            687..995
                     /locus_tag="VGM24_11885"
     CDS             687..995
                     /locus_tag="VGM24_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4156127.1"
                     /translation="MEKIEKLHAFLQDSPDDPFLKHALALEYIKAGEDGQALRLFMEI
                     LSRDPDYVGSYYHLAGLLERTGDREAAIGWYEKGMIAAKKKGDQHSFNELRSAYEDLI
                     "
     gene            1004..2359
                     /gene="tilS"
                     /locus_tag="VGM24_11890"
     CDS             1004..2359
                     /gene="tilS"
                     /locus_tag="VGM24_11890"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY4156128.1"
                     /translation="MNNLIFRFQESVKQQQLFGKADRLLLAVSGGMDSVVLAECCYRS
                     GFDFSIAHANFRLRDAESERDEQFVKELAAGYQRPFLVKRFNTREYAAENSLSIQVAA
                     RNLRYSWFKFLLGNEPAGYRFLLTAHHLDDNIETMLMQFFRGTGIAGLRGMVPRQEKI
                     IRPLLAFPKSDLRMFAEDQKLSWVEDSSNESDKYSRNYFRNTLIPAVEKVFPETRHNL
                     EGNLRRFAETETLYLQAIQWHKKKLLKPVGEEIHIPILLLKKSVPLHTILFEILHEYG
                     FSPAQVEDCIRLMESGNGKYISGSSRRIIKNRNWLIIAPLQDAGAAHIIIEKDDPVVP
                     FPEGELIFKFFKAGANAEIEKNLSLALLDADRINFPLILRKWKAGDYFYPLGMKKKKK
                     IARFLIDRKLSKTAKEKVWVLEMDQRIIWVLGQRIDNRFCLSPGTKEILKIEMRMPHF
                     R"
     gene            complement(2324..>2720)
                     /locus_tag="VGM24_11895"
     CDS             complement(2324..>2720)
                     /locus_tag="VGM24_11895"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY4156129.1"
                     /translation="YMGMLGLQNYYYLALYIGALIVSNIPTFLKHRNDYNYRSLGASG
                     AVSAVLFAFILLKPWVTIYLIFIPIPAIIYGVLFLVYSAIMSRKGGDHINHDAHFYGA
                     LFGILFTIILHPDVVNVFLAEMRHPHFDF"
BASE COUNT          746 a          653 c          682 g          639 t
ORIGIN      
        1 gcgggcgccc ataatgcctt tcatattagg gatccgttgt tccgccattc ctttctggca
       61 actgaccacc aggggcaggc tgacttcttc cgtttcttcg ccggcttcca cttcccttac
      121 cacggtggcc gtattgccgg ccagatcaaa ttttgccgcc agggaaacat aggggaggtc
      181 aagtattccg gcaatcattc caccgacgga agatccgttg taatcgatcg tttcctttcc
      241 ggtgaaaata agatcgtatc cggctccggc agcaaccgcc gcgatctggg aagcaataaa
      301 aaaactgtcc tgactatccg catttacccg gatggcctcg tcgcccccca atgccagcgc
      361 tttccgcagg atggcatcgg cgtcggcgcc gcctaccgta atcagatgca gcaccaggcc
      421 cggatctttt tctttcagct caatggcacg caccagggca taccattcat catacggatt
      481 gatgatccac tgaacgcctt cggcagcaaa tttcctgttg ttatccgtga aggctatttt
      541 tgccgtggta tccggcgttt tactgataca tactaatatc ttcatacgaa tggttggctg
      601 aattaaggtt gtttatgcaa ctaaaacaaa ggtaagccct tgatttgaaa agaaaattaa
      661 taaataaaaa ataaaatcag gcgtatatgg agaaaataga aaagctgcac gctttcctgc
      721 aggattctcc tgatgatccg tttttaaagc acgcgctggc actcgaatat attaaagcgg
      781 gcgaagacgg gcaggccctc cggttgttta tggaaatact gagccgcgat cccgattatg
      841 tcggatcgta ctatcatctg gcgggcctat tggaaagaac cggggaccga gaggcggcga
      901 tcggctggta cgaaaaagga atgatcgcag caaagaaaaa aggcgaccag cattctttta
      961 acgaattgag atcggcatac gaagacctca tttaataagg accatgaaca atctgatttt
     1021 tcgttttcag gaatcggtaa aacagcagca gcttttcggc aaagcagatc gtttgctcct
     1081 ggccgtgagc ggaggaatgg attctgtggt tttggcggaa tgttgttacc ggtccggctt
     1141 tgatttcagc atagctcatg cgaatttccg gttgcgggat gcggaaagcg aacgcgatga
     1201 gcaatttgtg aaggaactgg ctgccggata tcagagaccc tttttggtca aacgatttaa
     1261 cacccgggaa tacgctgcgg aaaattcctt atccatacag gtggcggcca ggaatctgcg
     1321 ctattcgtgg tttaaatttt tactggggaa tgagccggcc ggctaccggt tcctcctgac
     1381 agcccatcac ctcgatgata atatcgaaac gatgctgatg caatttttcc ggggaaccgg
     1441 catcgccgga ttgcggggaa tggtacccag acaggaaaag attatccggc ccctgctggc
     1501 gtttccgaaa tccgacctcc ggatgtttgc ggaagatcaa aaattgtcct gggtggaaga
     1561 cagcagcaac gaatcggata aatacagcag gaattatttc cggaataccc tgatcccggc
     1621 cgtggaaaag gtttttccgg aaacccggca caacctggaa ggaaacctcc ggcgctttgc
     1681 ggaaacggaa accttatacc tgcaggccat acagtggcac aaaaagaaat tgctgaaacc
     1741 ggtgggagag gagatccata tccctattct cttactaaag aaatcagtcc cgctccatac
     1801 cattctattt gagatcctgc atgaatatgg tttttctccc gcccaggtgg aagactgcat
     1861 ccggctgatg gagagcggga acgggaaata catatccggc agttcccggc ggatcattaa
     1921 aaacaggaac tggctgatca tcgctccgct acaggatgcc ggggcagcac acatcattat
     1981 cgaaaaggac gatccggtgg ttccctttcc cgaaggagaa ttgattttta aatttttcaa
     2041 ggccggggcc aatgccgaaa ttgaaaaaaa tttatccctg gcgttgctcg atgccgacag
     2101 gataaatttc cccctgatcc tgagaaaatg gaaagcaggg gactatttct atccgctcgg
     2161 tatgaaaaag aaaaagaaga tcgcccgctt tctgatcgac cggaaattat ctaaaacggc
     2221 caaggaaaaa gtctgggtgc ttgaaatgga ccagcgcatc atctgggtgc tgggtcagcg
     2281 gattgacaac cgtttttgcc tttcccccgg aacaaaagag attttaaaaa tcgaaatgcg
     2341 gatgccgcat ttccgctaag aacacattga ccacgtccgg atgcaatata atcgtaaaca
     2401 ggatcccaaa gagcgcacca taaaaatgcg catcgtggtt gatatggtca ccccccttcc
     2461 tcgacataat ggcggaataa accaggaaga gaacaccgta aataattgcc ggtatgggaa
     2521 taaaaatcaa atagatggtg acccagggct ttaggagaat gaacgcaaaa agcacggccg
     2581 ataccgcccc ggatgctccc agacttctgt agttataatc attccggtgt ttcaggaagg
     2641 tgggtatatt ggatacgatc agcgcgccga tatacagcgc caggtaatag taattctgca
     2701 ggccgagcat gcccatatag
//
LOCUS       DASYCU010000589         3906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4042398, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000589 DASYCU010000000
VERSION     DASYCU010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4042398"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2527
                     /gene="ileS"
                     /locus_tag="VGM24_11900"
     CDS             <1..2527
                     /gene="ileS"
                     /locus_tag="VGM24_11900"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY4156130.1"
                     /translation="VNVVLARALLGKYFKPEEENGEFTTYGPSSKSLPWKILLSFRGS
                     DMEGCRYEQLIPTEAGSAANILRITPDADPFRVITGDFVTTEDGTGIVHTAPAFGADD
                     YKAGKKNKLGILILVDKRGKFVDDAGEFSGRYVKNYLDDPHYTDVNVDICVKLKKENR
                     AFKIEKYEHTYPHCWRTDKPILYYPLDAWFIRTTAFRDRMVELNKTIQWKPASTGEGR
                     FGNWLENMVDWNLSRSRFWGTPLPIWRTEDGKEEKCIGSIEELLEEARKARTKLGGEI
                     NAEVNAAALDLHKPFVDRIILLSESGKPMTRVADLVDVWFDSGAMPYAQWHFPFENKA
                     QFEKNFPADFIAEGVDQTRGWFYTLHALGVMLFDQVAYRTVVSNGLVLDKNGNKMSKR
                     VGNVVDPFKTIDQFGADATRWYLITNASPWDSMKFDTEGIKEVQRKFFGTLYNTYQFF
                     TLYANVDGFDFKEKYIPAEERPEIDKWILSALNSLIRKVRDSFDEYEPTVAGRLIEDF
                     VDQQLSNWYVRLSRRRFWKGEYGQDKLCAYQTLYECLETLSKLIAPVSPFFADWLFLN
                     LNGATGRSSRLSVHHTDFPEVNSRFIDEKLEERMQMAQDVSSLILSLRKKINIKVRQP
                     LQKVLIPVLDPGMTEQLKKVEALVKSEVNVKELSYLADTDGFISKKIKPNFVMLGKKL
                     GAKMKAVSNALAQFGQPEISRMEKEGGTAVSVEGEMVWIALNEVEITSEDVPGWMVAS
                     RGPLTVALDVTVTPELEKEGNARELVNRIQKIRKDSGFELTDRINVQIGGAETLQETI
                     RQYNYYICAEILADQLEMVPQVSNGTLIEVNEIPLTVFVTKKA"
     gene            complement(2528..2761)
                     /locus_tag="VGM24_11905"
     CDS             complement(2528..2761)
                     /locus_tag="VGM24_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156131.1"
                     /translation="MDELAEALDADFTGTDFAATGFLVTGTFFLCISFLTGFDGAGLL
                     AAAGFFEAGFAAFATFFGSAFFTDFTGFFLIAI"
     gene            2841..3476
                     /locus_tag="VGM24_11910"
     CDS             2841..3476
                     /locus_tag="VGM24_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY4156132.1"
                     /translation="MAAKPAQPVAAVKAPAARTNEIKKPVFKQEVKNTSPVTAKPEPK
                     KPVPPPARTGKLVIPEIKTKTVRKYEPEFTKSVLDTHQQQQTGPTMRYSDTELTEFRE
                     LITKKLEAAKKELAYLQGLITRRDEMGGDETDNRYMTMEDGSMSMEREQLSQMASRQI
                     TFIDHLEKAMMRIENKTYGICRVTGKLIDKARLKAVPHATLSIEAKQTMNK"
     gene            3496..>3906
                     /locus_tag="VGM24_11915"
     CDS             3496..>3906
                     /locus_tag="VGM24_11915"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY4156133.1"
                     /translation="MQSRLFFILMALALGAILPLQAAINTRLAKTADHNPVMAAFVSF
                     SVGTIALMIFLITAGRFNFRFVSTGSPWWIWVGGLLGTFFVAGIVILLPKLGAALAFS
                     LVIAGQMLAAVLFDQFGWMGLAVKEISAGKIAGAI"
BASE COUNT         1103 a          897 c         1059 g          847 t
ORIGIN      
        1 ggtgaatgtt gttcttgccc gggccctgct gggaaaatat tttaaaccgg aagaagaaaa
       61 cggggagttt accacctacg ggccttcatc caaatccctt ccctggaaaa tactgctttc
      121 atttcgcggt tcggatatgg aaggatgccg gtacgaacag ttgatcccca cggaagcggg
      181 aagcgccgcg aatattctcc ggatcacgcc cgatgcggac ccgttccggg tgataaccgg
      241 agattttgtt accacggaag atggaaccgg aattgtccac accgctcccg cctttggcgc
      301 ggacgactat aaagccggga agaaaaataa gctgggcatc ctgatcctgg tggataaaag
      361 aggaaaattc gtggacgatg caggagaatt cagcggccgc tacgtaaaga attacctgga
      421 tgatccgcat tacacagatg tcaatgtgga catctgtgtt aagctgaaaa aagaaaaccg
      481 tgcatttaag atcgaaaaat acgaacatac ctatcctcat tgctggcgga cggacaagcc
      541 catcttatat tatccgctgg acgcctggtt tatccggacc actgccttcc gggaccggat
      601 ggtggagttg aataagacca ttcagtggaa accggcttcc accggagagg gccgttttgg
      661 aaactggctg gaaaacatgg ttgactggaa cctgagccgg agccggttct ggggaacgcc
      721 cttgcccatc tggagaacgg aagacgggaa agaagagaaa tgcatcggtt caatcgaaga
      781 attgttggag gaagcccgga aagcgcgcac aaaactcggc ggcgaaatca atgcggaggt
      841 taatgccgcc gcgctggacc tgcacaaacc atttgtggac cgcatcatat tattaagcga
      901 atcgggaaaa ccgatgaccc gcgtggcgga cctggtggac gtctggtttg attcgggcgc
      961 catgccgtat gcgcagtggc attttccgtt tgaaaataaa gcgcagtttg aaaaaaattt
     1021 tccggccgat tttattgcgg aaggggtgga tcagacccgc ggctggtttt atactctgca
     1081 tgcgcttggg gtcatgcttt tcgaccaggt agcctaccgg acggtggtgt ccaacggact
     1141 ggttctggat aagaacggaa ataagatgag caagcgggtg ggaaatgtgg tggacccatt
     1201 caaaacgatc gatcagtttg gcgcagatgc gacccgctgg tacctgatca ccaacgcatc
     1261 cccctgggac agcatgaagt tcgatacgga aggcatcaaa gaagtgcagc gtaaattttt
     1321 cggcacctta tataatacgt atcagttttt caccctctat gccaacgtgg atgggtttga
     1381 ttttaaggaa aaatatattc cggcggagga aaggccggaa attgacaaat ggattctttc
     1441 cgcgctgaac agcctgataa gaaaagtaag ggacagtttt gatgaatatg aaccaacggt
     1501 ggcaggcaga ttgatcgaag attttgtgga tcagcagttg agtaactggt atgtcaggct
     1561 cagccgccgg cgcttctgga aaggagaata tggccaggat aagctttgtg cttatcagac
     1621 cctgtatgaa tgcctggaaa ccctgagtaa gctgatcgcg ccggtttctc ctttcttcgc
     1681 agactggctc tttctgaacc tgaacggagc aaccgggagg agcagccggc tatcggttca
     1741 tcacacggat tttccggaag tgaacagccg gttcatagat gaaaagctgg aagagcggat
     1801 gcaaatggca caggatgtat cctcgctgat cctttccctc cggaaaaaga tcaatatcaa
     1861 agtacgtcaa cccctgcaaa aggtattgat accggtgctg gatcccggga tgaccgaaca
     1921 actcaaaaag gtagaagccc tggtcaaatc ggaggtcaat gtgaaagaac tgtcttattt
     1981 agctgatacg gatgggttta taagcaaaaa gataaagccg aattttgtca tgctgggtaa
     2041 gaagctgggc gctaaaatga aagcggtcag caatgccctg gcgcagttcg gacagccgga
     2101 aatcagcagg atggaaaagg agggcggaac ggctgtttcg gttgaaggag aaatggtctg
     2161 gattgcttta aatgaggttg aaattaccag tgaggatgtg ccgggctgga tggtcgcttc
     2221 cagggggccc ctgacggtcg cattggacgt aacggtgacc ccggaactgg agaaggaagg
     2281 caatgccagg gaactggtga accggatcca gaagatccgc aaggacagcg gttttgaact
     2341 gaccgaccgg attaacgtac aaattggcgg agcggaaaca ttgcaggaga ctattagaca
     2401 atataattat tatatttgcg cggaaatttt ggcagaccag cttgaaatgg tgcctcaagt
     2461 ttccaatggc actttaattg aagtgaatga gattccttta acggtgttcg ttacaaaaaa
     2521 agcctaatta tatggcaatc aagaaaaaac ctgtaaaatc cgtgaaaaaa gcgcttccga
     2581 aaaaggtggc aaaggcggca aagccggctt caaaaaagcc cgcagccgct aaaagacctg
     2641 cgccgtcaaa acctgttaag aaggaaatac acaaaaaaaa cgtacccgtg accaagaaac
     2701 ccgtagccgc aaagtcagta ccagtaaaat cagcgtccaa agcctccgcc agttcatcca
     2761 agaaatcgcc tgttcccgcg gcatcaaaaa aaccggtttc ggctcatccc ggaacaaagc
     2821 cggtgacaca ggcagctccg gtggctgcaa aaccagccca gcctgttgct gccgtaaaag
     2881 ctccggctgc ccggacgaat gagataaaaa aaccggtatt taagcaggaa gtaaagaata
     2941 cctctccggt aaccgccaaa ccggaaccga aaaaaccggt tcctcctccg gcgagaaccg
     3001 gaaagttggt tattccggaa ataaagacaa aaaccgttcg caaatacgaa ccggagttta
     3061 caaaatccgt attagacacg caccagcagc aacaaaccgg acccaccatg agatatagtg
     3121 atacagagtt aacagaattc agggagttga tcaccaaaaa gctggaggct gcaaaaaagg
     3181 agctggctta tctccaggga ctgatcaccc gccgcgatga aatgggagga gatgaaacag
     3241 acaaccgtta tatgaccatg gaggatggca gcatgagtat ggaacgggag caactgagcc
     3301 agatggccag ccgccagatt acctttattg accatctcga aaaggccatg atgcggattg
     3361 aaaataaaac ctatggcatt tgcagggtga ccggcaagct gattgataaa gcaaggctga
     3421 aagccgttcc gcatgccact ctgagcatcg aggccaaaca aacgatgaac aaataatttc
     3481 tgttcatttg caaatatgca atcccgcctt ttttttatcc tgatggcgct ggcattggga
     3541 gcgatccttc cactgcaggc ggctattaat acccgcctgg caaaaacggc cgaccataat
     3601 ccggtcatgg cggcatttgt ttccttttcc gtgggaacaa tcgccctgat gatttttctg
     3661 atcaccgccg gccggtttaa ttttcgtttt gtttccaccg gctcgccctg gtggatctgg
     3721 gtaggcggtc tgctgggtac cttcttcgtt gccggaattg tgatcctgtt gcctaagctg
     3781 ggcgcagccc tggcgttcag cctggtgatt gccggacaaa tgctggcagc ggttttattc
     3841 gaccagttcg gatggatggg gcttgccgtg aaagaaattt ccgcaggtaa aattgccggc
     3901 gccatt
//
LOCUS       DASYCU010000590         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_418003, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000590 DASYCU010000000
VERSION     DASYCU010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418003"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2932)
                     /locus_tag="VGM24_11920"
     CDS             complement(<1..2932)
                     /locus_tag="VGM24_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEY4156134.1"
                     /translation="MNKALRKILAFSLKNKYFIYFATCIMVIYGVITFINMPIEAFPD
                     VTNTEISIITQWPGRSAEEVEKFVTIPLEIALNPVNRKTSLRSTSIFGLSSVKLTFED
                     GVDDSYARVQVNNMIRDVSLPENISPQIQPPTGPTGEVFRYTLESSFRDPMELKTIQD
                     WTVDRELRSVPGIADIVSFGGSVKSYEIRVNPQKLTGLGITPLDVYTAVSKSNINIGG
                     DVIKKNAQAYIVRGIGLLNDINEIRNIFIANINGIPVLAKDVADIQVAAMPRLGEVGR
                     SDAIDSGSSRKLTDVPDAVEAIVLMRKGENPDKVIKALRLKIQKLNDRVLPPDVKIVP
                     FYNREELIRYATHTVMHNLIEGILLVTLIVSLFMFNWRTTLIVSIIIPLALLFSFICL
                     HFMGMSANLLSLGAIDFGIIIDGAVVMVEGLFVMLDQLAKEHSMERFNKLAKMGRIRK
                     KGAELGKAIFFSKVIIITALIPIFSFQKVEGKMFSPLAYTLGFALLGALIFTLTLVPV
                     LVNALLSKNVREKHNPFTHAMTGWVMSGFRFVFHNKRLSLAISAIVIVLGLYAFKFLG
                     SEFLPELNEGSIWVRATMPYSIALEKSVQVASQMRSIMMEFPQVRKVVSQTGRPDDGT
                     DVTGFYNNEFDVSLYPQEQWKPKISKNELIEKMRKALSVIPGAELNFSQPIMDNVEEA
                     VSGVKGSICVKIFGDSLNYMEARSDEVYNILKKIRGIEDLGVIRNIGQPELDIDLDQS
                     RMALYGVSAADANAVIEMAIGGKAVTQLYEGVRRFDIRIRFPEEFRSSETDIGNLLVP
                     TSGGSKVPVREIADITLKTGPCLIFRDDNERYSAIKFSVAGRDMGSTIKEAQGKVDEQ
                     VKLKRGYNLVWQGDFENQQRASKRLLEVVPISLLLIFLLLFIMFGNLKDAGLVFLNVP
                     FAVVGGILALLLTGTNFSISAGIGFIALFGICILEGVLLITAFKQNLERLRGENPLFT
                     SVKRG"
     gene            complement(2948..>3366)
                     /locus_tag="VGM24_11925"
     CDS             complement(2948..>3366)
                     /locus_tag="VGM24_11925"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4156135.1"
                     /translation="ITSVKSGDSVQVTTLSYPGKIFRGKIDKILNVLDPTNKVMKLRI
                     VLPNPGYLLKPEMFASVLVRNTENKKMISIPSRALIFDHSQYYVLIYKSPSDITIRPV
                     QVMNTVGDQSFISGGLTQGEKLIGTQALLIYQELNS"
BASE COUNT          803 a          860 c          835 g          868 t
ORIGIN      
        1 cgcctctttt cacagaggtg aacagcggat tctccccgcg cagcctttcc aggttttgtt
       61 tgaatgcggt gatcagcaac accccttcaa ggatacaaat cccgaacagg gcgataaatc
      121 cgataccggc ggagatacta aaattagtgc cggtaaggag cagggccagt attccgccca
      181 ccacggcaaa gggaacgttg agaaacacga gtcccgcatc tttcaggttt ccaaacatga
      241 tgaatagcag gaggaagatg aggaggagac tgatcggtac tacttccagc aaccgtttgg
      301 atgcccgctg ctggttttcg aaatcgccct gccagacgag attatagcct ctttttaatt
      361 ttacctgctc gtccacctta ccctgcgctt cctttatagt gcttcccatg tcgcggccgg
      421 caacggaaaa tttaatggcc gaataccgtt cattgtcgtc ccggaaaatc aggcagggac
      481 cggtttttaa ggtgatgtcc gcaatctccc ttacgggaac tttagaaccg ccggaagtcg
      541 gcaccagcag gttgccgata tcggtttccg agcttctgaa ttcttccggg aaacgaatgc
      601 ggatatcgaa ccgccgcacg ccttcatata attgggtcac cgcttttccg ccgatcgcca
      661 tttcaattac cgcattggca tcggctgccg atacgccata cagggccatg cggctctgat
      721 ccaggtcgat gtccagttcg ggctggccga tattgcggat cacgcccagg tcctcgatcc
      781 ctcttatctt tttcaggata ttgtaaacct catcggatct ggcttccatg taattcaacg
      841 aatcgccaaa tattttcacg cagatagatc ccttaacacc cgatacggcc tcttccacgt
      901 tgtccatgat gggttgggag aaattgagct cggcgcccgg aataacggaa agcgctttcc
      961 tcattttttc gatcagttcg ttcttcgata ttttcggctt ccattgctcc tgggggtaaa
     1021 gggatacatc gaattcatta ttgtaaaacc cggtcacgtc ggttccgtcg tccggccttc
     1081 cggtttgtga gaccactttc cgaacctgtg ggaattccat cattatgctg cgcatctggc
     1141 tggccacctg gaccgatttt tccagggcga tgctataggg catggtggcg cgcacccaga
     1201 tggatccttc attcagttcc ggaaggaatt cgctacccag gaatttaaac gcatatagtc
     1261 ccagcacaat cacaatggcc gatatggcga gcgacaatcg tttattatga aatacaaaac
     1321 ggaatccgct catcacccaa ccggtcatgg catgggtgaa agggttgtgt ttttcgcgta
     1381 cgttcttact taataaagca ttgaccagca ctggcaccag ggtcagcgtg aagatcaggg
     1441 cacccaataa ggcaaatccc agggtataag ccagcggaga aaacattttc ccttccactt
     1501 tctgaaagga aaaaataggg atcagggcgg taatgatgat cactttcgaa aagaaaattg
     1561 ctttgccgag ttccgcccct tttttcctga tcctgcccat cttggccagt ttgttaaacc
     1621 gttccatgct gtgctctttt gccagttgat ccagcatcac gaacaacccc tccaccataa
     1681 ccaccgcgcc gtcaataatg atcccgaagt caatggcgcc aagcgaaagc aggttggcgg
     1741 acatgcccat aaaatgcagg caaataaaag agaacagcag ggccagcgga atgatgatgg
     1801 atacgatcag ggtcgttcgc cagttgaaca tgaacaggga aacgatcagg gttacgagca
     1861 ggatgccttc aataagattg tgcatcaccg tgtgcgtggc ataacggatc agttcttcgc
     1921 gattgtaaaa aggaacgatc tttacatccg gaggcaatac ccggtcgttc agcttctgaa
     1981 ttttgagccg gagggcttta atcactttat ccggattttc tcctttgcgc atcagcacga
     2041 tggcttccac ggcatccgga acatcggtga gcttcctgct gcttccgctg tctatggcat
     2101 ccgatctgcc cacttcgccc agccgcggca tggcggccac ctggatatcg gcgacatctt
     2161 tcgcgagcac gggaatgcca ttgatattgg cgatgaaaat attccggatc tcattgatat
     2221 cattcagcaa cccgatcccg cggactatat atgcctgcgc atttttcttg atcacatctc
     2281 cgccgatatt gatattgctt ttgcttaccg ccgtatatac atccagcggc gtaatgccaa
     2341 gcccggtgag cttttgcggg ttcacgcgga tctcatagga tttaacggat cctccgaagc
     2401 tgacgatatc ggcgatcccg ggaaccgagc gcagttcccg gtccacggtc cagtcctgaa
     2461 tcgtttttaa ttccatggga tcccggaacg aactttcgag ggtgtaacgg aacacttcac
     2521 cggtggggcc cgtaggcggt tggatctggg gagaaatatt ttcgggaagg cttacgtccc
     2581 ggatcatatt gttcacctgg acccgcgcat agctgtcgtc aaccccgtct tcaaaagtca
     2641 atttgacgga agagagtccg aagatactgg tggaccggag actggttttc cggtttacgg
     2701 gattgagcgc aatttccagc gggatggtta cgaatttttc tacttcttcg gcgctccttc
     2761 ccggccactg ggtgataatc gagatttccg tattggtcac atcgggaaat gcttcgatcg
     2821 gcatattgat gaaggtgatc acgccataga tcaccatgat gcaggtggca aaataaatga
     2881 agtatttgtt cttaagggag aaggcgagta ttttccgcag ggccttgttc atctatgaat
     2941 ggttaatcta actgttgagt tcctgataaa ttaataatgc ctgggttccg atcagttttt
     3001 ccccctgcgt taaacctccg gagataaagg actggtcgcc gaccgtattc ataacctgta
     3061 caggccggat cgtgatgtcc gaagggcttt tatagatcaa tacataatac tggctgtggt
     3121 caaagatcaa tgcccgggaa gggatgctga tcatcttttt attttcggta ttccggacca
     3181 gcacgctggc gaacatttcc ggcttgagca gataacccgg attgggcagc acaatgcgga
     3241 gcttcatgac cttgttggta gggtccagca cattcaggat cttatctatt ttcccgcgga
     3301 aaattttccc gggataggag agggtagtca cctgtacgct gtcgccgctt ttaacactgg
     3361 taatat
//
LOCUS       DASYCU010000591         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3027871, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000591 DASYCU010000000
VERSION     DASYCU010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3027871"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGM24_11930"
     CDS             complement(<1..533)
                     /locus_tag="VGM24_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY4156136.1"
                     /translation="MSTRNLSLLIILAVILILGGCACSGYNSLVSKDENTKGKWGNVQ
                     SDYQRRSDLIPNLVNTVKGAANFEQTTLTQVIEARAKATQVQVNANDLSPEKIAQFQQ
                     AQAGVTGALGRLLAVAEAYPTLQATQNFRDLQAQLEGTENRIKVSRNDFNQAVQDYNQ
                     TARTFPNNLFAGMFGFKA"
     gene            complement(612..1814)
                     /locus_tag="VGM24_11935"
     CDS             complement(612..1814)
                     /locus_tag="VGM24_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY4156137.1"
                     /translation="MSTFSQHDFKGQKALVRVDFNVPLNDRMEITDDTRMRAVVPTIQ
                     KILKDGGSAILMSHLGRPKDGPSNKFFLKHLVGHLSELLGGIPVKFAEDCIGEQARLD
                     AERLKPGEVLLLENLRFYKEEEKGDEAFAKKLARYGDVYVNDAFGTAHRAHASTAVIA
                     KFFPKDKRMFGLLMESEVTSAEKVLHAARKPFVAIIGGAKVSDKILIMENLLEKATDI
                     IVGGGMAYTFLKAQGGKIGNSLCENDRLQTALDLLAKAESKGVCIHLPADSIIADKFD
                     AHAETSASPSNQIPDGWMGLDIGPNACEQFCNVIRKSKTILWNGPMGVFEMENFRHGT
                     RTIAIAVAEATKNGAFSLVGGGDSVAAVNQFGLAGKVSYVSTGGGAMLEYFEGRELPG
                     IAAINNPS"
     gene            complement(1887..>2471)
                     /locus_tag="VGM24_11940"
     CDS             complement(1887..>2471)
                     /locus_tag="VGM24_11940"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY4156138.1"
                     /translation="ETVISCASCTTNCLAPMADTLDKKFGIELGFMTTVHAYTNDQNT
                     QDAPHPKGDLRRARAAAENIVPNSTGAAKAIGLVLPNLKGKLDGNAQRVPVLTGSLTE
                     LTSILKKKVTVAEINTAMKEAGNESFGYTEDEIVSSDIVGMHFGSLFDATQTKVITVG
                     DKQLVKTVSWYDNEMSYVSQLVRTVHYFATLISR"
BASE COUNT          507 a          661 c          652 g          651 t
ORIGIN      
        1 gccttgaatc caaacatgcc ggcaaacagg ttattcggga aggtcctcgc cgtctggttg
       61 tagtcctgga cggcctggtt gaaatcgttt cgggacacct tgatccggtt ttccgttcct
      121 tccagctgcg cctgcagatc cctgaaattc tgggtcgcct gcagggtggg ataggcttcc
      181 gccaccgcaa gcagtcttcc caaagctccg gttacgcccg cctgggcctg ctggaactgg
      241 gctatttttt cgggagaaag atcattggcg ttcacttgta cctgggtggc ttttgccctc
      301 gcttcgatca cctgcgtgag agtggtctgt tcaaaattgg cagcaccttt taccgtattg
      361 accaggttgg gtatgagatc ggaacgtctt tgataatcgc tttgcacgtt cccccattta
      421 cccttggtgt tctcatcctt gctcaccagg ctgttatagc cgctgcaggc gcaaccgcct
      481 aaaataagaa tgacggccag tataataagg agtgataaat tacgcgtact cataagttga
      541 agggtttgtt tagctgttaa atgtaacaat ttccacagaa agatgttctg tgccaacagg
      601 ttatttaatc cctaggaagg attattgatc gctgctatgc cgggaagctc cctgccttcg
      661 aaatattcca gcatggcgcc tccgccggtg gaaacataac tgaccttgcc ggcaagaccg
      721 aactggttga ccgcagctac ggaatctcct ccgccaacca gcgaaaaagc accgtttttt
      781 gtagcctccg ccacggcgat ggcaatcgtc cgggtgccgt gccggaagtt ttccatttcg
      841 aaaaccccca tgggcccgtt ccagagaatg gtctttgatt tccggatcac gttacaaaac
      901 tgttcgcagg cgttaggtcc gatatccagt cccatccagc cgtccggtat ctggttgctg
      961 ggcgaagcgg aggtttccgc gtgagcatcg aatttatcgg cgattatgga atctgcgggc
     1021 aggtgaatgc aaacgccttt ggattcggct ttggcaagca ggtcgagcgc cgtctgaagc
     1081 cggtcgttct cgcagaggga attgccgatc ttgcctccct gggctttcag aaaggtatac
     1141 gccattccgc cgccgacaat gatgtcggtc gctttttcca gcaggttttc catgatgagg
     1201 atcttatcgg agacctttgc gccgccgatg atggccacaa agggttttct ggccgcatgc
     1261 aatacttttt ccgcgctggt cacttcggat tccatgagca ggccgaacat tcttttatcc
     1321 ttcggaaaaa acttcgcgat aacggcagtg gatgcgtgtg cgcggtgagc ggtgccgaaa
     1381 gcatcattca catagacatc tccgtaccgg gccagttttt ttgcgaatgc ctcatctcct
     1441 ttttcttctt ccttataaaa acggaggttt tccagcagca gcacttctcc cggtttgagc
     1501 cgttccgcgt ccagccgggc ctgctctccg atgcagtcct ccgcaaattt gaccggaatg
     1561 cctccgagca gttccgacaa atggccgacc aggtgtttga ggaaaaactt gttggaaggc
     1621 ccgtcttttg gccgtcccag gtgcgacatc agtatggcgc tgccgccatc tttcaggatc
     1681 ttttgaatgg tgggcaccac ggcccgcatc cgggtatcgt cggtgatttc catccggtcg
     1741 ttcaggggca cgttgaaatc cacccggacc agggctttct gtcctttgaa atcatgctgg
     1801 gaaaaagttg acatgctgcg caatatttaa ggaatggata taaaaaatga tgccggaaca
     1861 attccggcat ccaggcgata aaaattttac cggctgatca gcgtggcaaa ataatggacg
     1921 gtgcgcacca gctggctgac ataactcatt tcgttgtcgt accagctcac tgtttttacc
     1981 agttgtttat ctcccaccgt gatcactttg gtttgggtgg catcaaaaag ggagccgaaa
     2041 tgcattccta cgatatcgct gctcacgatc tcgtcttccg tatatccgaa gctctcattt
     2101 ccggcttctt tcatggccgt attgatctcg gcaaccgtca ctttcttctt cagaatgctc
     2161 gtgagttcgg tgagcgatcc cgtcagcacg ggaacgcgct gcgcatttcc gtcgagtttc
     2221 cccttgagat tgggaagaac cagcccgatc gccttggccg cgccggtgct gttgggaacg
     2281 atgttttccg ctgcggccct ggccctgcgc agatctccct tgggatgcgg cgcatcctgg
     2341 gtattctgat cgttcgtata ggcatgaacg gtagtcatga agccgagttc gatgccgaat
     2401 ttcttatcga gggtatccgc catgggagcc aggcagttcg tggtgcagga agcgcagctg
     2461 atcaccgttt c
//
LOCUS       DASYCU010000592         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2162025, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000592 DASYCU010000000
VERSION     DASYCU010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162025"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VGM24_11945"
     CDS             <1..777
                     /locus_tag="VGM24_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY4156139.1"
                     /translation="RWLDEQCIHCDNPTQALEIDDLLSLALSGERMKAIALRDPARLY
                     NASQDSFFKQILQSGNAEPRADARSYLYQVMAETISGAGYIFEQSKLRPGNTVYPATE
                     IGKNLKTISSLICSDINTRVYYLSLGSFDTHVGQEERQKKLFSEMNEAIKTFISDLKA
                     SHRFDQVLLMTFSEFGRRVSQNASGGTDHGTANNMFLISGGLKRAGLLNAMPDLSNLA
                     GGDLRYRIDFKNLYATVLKKWLEADDRKILGREYECLDFI"
     gene            complement(770..>2264)
                     /locus_tag="VGM24_11950"
     CDS             complement(770..>2264)
                     /locus_tag="VGM24_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY4156140.1"
                     /translation="ALDNYGNIEDCQWHTFQITWDASTTTLSAAIDGIPRVQTKTDLV
                     KDIFHNNPEVFWGFSAATGGESNVQKFCTSLNPGYSLPADQKACAPATLTFKDSSLSF
                     GSILKWSWDFGDGTTYDGGPAPPPHDYPDPGYYTVKLNILGNNGCLSDTFMHKITIGS
                     KPSAGFTNLPKTICANSPAILYDASRVDYGSINEWNWNFNAGESQVQQSVPQLPWTFA
                     AGSQQIQLTVLTEQGCVSDPYSVTMNLMDKPQTHMSVEDACYGEPAHLNGENINPAVP
                     VRQWYWFTGDGGFDSSAQVIHYYPLAGKYPVKLYAENDAGCSSDTLQGLVTIYQTFAR
                     AGKDTVVAIGQPLQLHASGGDLYRWLPSTGLDDPFSADPVAILSTDMQYVLTAYTAFG
                     CPSYDTIRVKAYKGPDIYVPNAFTPDNNGHNDRFHAIAPGMANIDYFNIYNRSGQLLY
                     SSHNSSEGWDGTRNGQPQPPGTYIWMIKGSDYQGKIHAKKGVVVLIR"
BASE COUNT          556 a          546 c          605 g          557 t
ORIGIN      
        1 agatggctgg atgaacaatg tattcattgc gataatccga cgcaggcgct tgaaatcgat
       61 gacctgctta gcctggccct gagcggagag cggatgaaag ccattgccct ccgggatccc
      121 gcgagactct ataacgccag ccaggattca ttcttcaaac aaatcctgca atcgggcaat
      181 gcggagcccc gggcggatgc gcgttcttat ttataccagg tgatggcgga aacgatttcc
      241 ggcgccggct atatttttga gcagagcaaa cttcgtcccg gaaatacggt ttatccggct
      301 accgaaatcg ggaaaaattt aaaaaccatc tcttccctga tctgctcgga tatcaatacc
      361 cgggtttatt atctgagttt gggaagcttc gacacgcacg tcggccagga agaacgccaa
      421 aagaaattat tttcagaaat gaatgaagcg ataaagacct ttatttcaga tctgaaagcc
      481 agtcatcgtt tcgaccaggt attgctgatg acattttccg aatttggaag aagggtatca
      541 caaaacgcca gcggtggaac ggatcatggc acggccaaca atatgttcct gatcagcggc
      601 ggcttaaaaa gagccgggtt gctgaatgca atgccagacc tttcaaatct tgccggagga
      661 gatctccggt accggataga tttcaaaaac ctgtatgcaa cggtattaaa aaaatggctg
      721 gaggcggacg accggaaaat cctcggcagg gagtacgagt gcctggattt tatctgatca
      781 gcacaaccac tccttttttt gcatggatct ttccctggta atcggatcct tttatcatcc
      841 agatataggt gccgggcggc tgcggttgcc cgttccgggt cccgtcccaa ccctcgctgg
      901 aattatgact gctgtacagc agctgcccgg agcggttgta aatattgaaa taatcgatat
      961 tggccattcc cggggcaatg gcatgaaaac ggtcgttgtg gccattgtta tccggcgtaa
     1021 acgcgttggg aacataaata tccggcccct tatatgcctt aacccggatg gtatcatagc
     1081 tgggacagcc aaaggcggta taagcggtca ggacgtattg catatccgta ctgagtatcg
     1141 ctaccgggtc cgctgaaaag ggatcgtcca gccccgttga cgggagccac cggtacaaat
     1201 ctccgcccga cgcatggagc tgaagcggct ggccgatggc aacgaccgta tcttttcccg
     1261 ctctggcaaa ggtctgataa atcgtaacga gaccttgtaa agtatcggat gaacatccgg
     1321 catcgttctc tgcatatagt tttaccggat acttcccggc cagggggtaa taatgaataa
     1381 cctgtgcgct gctgtcaaaa ccgccgtcgc cggtaaacca gtaccattgc cggaccggta
     1441 cggcagggtt gatattttct ccgttgaggt gggccggctc cccataacag gcatcctcaa
     1501 ccgacatatg ggtctgtggt ttgtccatca ggttcatggt tacagaataa ggatctgaaa
     1561 cgcatccctg ctccgttagg accgtgagtt gtatttgttg tgacccggcg gcaaaagtcc
     1621 acggcaattg cggcacagat tgctgaacct ggctttcccc cgcattgaag ttccaattcc
     1681 attcattgat cgagccgtag tctacgcggg aggcatcata aagtattgcc ggagaattgg
     1741 cgcagatcgt tttggggaga ttggtaaatc cagccgatgg ttttgaacca atcgtaatct
     1801 tatgcatgaa ggtatccgag aggcagccat tatttccgag tatattgagc ttgaccgtgt
     1861 aatagcccgg gtccggataa tcgtgaggcg gaggggccgg gccgccatca tacgtggtgc
     1921 cgtctccaaa atcccaggac cattttaaga ttgatccgaa agaaagcgaa ctatctttaa
     1981 aggtcagggt tgccggtgcg caggctttct ggtcggccgg cagggaatat cccggattca
     2041 gggaagtgca gaatttctga acattactct ctccgccggt ggctgcgcta aatccccaga
     2101 acacttcggg gttgttatgg aagatatctt ttaccagatc tgtttttgtc tgaacccggg
     2161 gtattccatc aatcgctgcg gaaagagttg tggtggatgc atcccaggtg atttggaagg
     2221 tatgccactg gcagtcttcg atattgccgt aattgtcgag tgcg
//
LOCUS       DASYCU010000593         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3898899, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000593 DASYCU010000000
VERSION     DASYCU010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3898899"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..735)
                     /gene="pdeM"
                     /locus_tag="VGM24_11955"
     CDS             complement(49..735)
                     /gene="pdeM"
                     /locus_tag="VGM24_11955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY4156141.1"
                     /translation="MQSPFPHKILNQQFWLTGDRTLFWEEEKALIVSDLHFGKTGHFR
                     KSGIAVPQSVYKEDLQRLIVQIQHFQPRQLIIVGDMFHSRANLELELFLKWRADLPDL
                     DIQLVKGNHDILKEDWYENAGIRLNRTTLSMNRFHFVHDISGCQYQDEGFTGHSRLAR
                     PYFFSGHIHPGIRIQGMGKQSLCFPCFYFGKEYAVLPAFSRFTGVALIDPEEEEQVFA
                     IVNNSIVRFQ"
     gene            complement(768..3347)
                     /locus_tag="VGM24_11960"
     CDS             complement(768..3347)
                     /locus_tag="VGM24_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEY4156142.1"
                     /translation="MALQQTKGYQVILEWFESSGLTPFAFQEETWEHIGEGHSGLVNA
                     PTGCGKTFAVFLGSLIHFINEHPEDYRTRSPNQLRLLWITPLRALAKDIGRAMTEVIA
                     GLGMTWRIGIRNGDTEISEREKQKRHMPEVLIITPESLHLLLAQKGYPEIFKGLKILA
                     VDEWHELLGSKRGVQVELAVSRIAHLIESYHAPFPGMADPVEIAGNKVFPFSVWGISA
                     TIGNLEQAREVLLSPLLAKMHFPPVTVKASIDKRIEIESVIPEEIEKYPWAGHLGIKL
                     VHKVIPIIEKSRTTLIFINTRGMSERWYQEILNVAPGLAGAIALHHGSVDRELRLWVE
                     EALHSGKLKAVVCTASLDLGVDFRPVETVIQVGSPKGVARFLQRAGRSGHQPGEISKI
                     YFLPTHSLELVEAAALKLAMGLNFIESREPMMLCFDVLIQYLGTLAIGEGFYPDQIFR
                     EISSTYCFRDITREEWNQILHFITEGGEALQQYDEFRKVEVIAGLYKITGRRVAMRHR
                     LHIGTIVSDAMLKVKFMSGGYVGVIEEYFISRLNPGDVFTLAGRNLEFVMIREMTAIV
                     KKSNAKKAIVPSWNGGRMPLSANLGRMLRKKFNEAWLVAEGREKSVPDIELRSLKPLF
                     ELQQKLSHIPKDDELLIEQIETADGFHLFVYPFEGRLVHEAMAALLAYRISRIVPISF
                     SFAMNDYGFELLSDQPIPLDDTNAHDLFSDEELSADIQRSVNSAEMARRKFRDIAVIG
                     GLIFQGYPGEHKKARHLQSSAGLLFNVFSEYDPHNLLLRQAYQEVMEQQMEEVRLRNM
                     LERIKESRIVISFPEKLTPFCFPLKVDSMREDLSSEKLEDRIKKMQEQLNKDHERKRV
                     NRS"
     gene            complement(3378..4760)
                     /locus_tag="VGM24_11965"
     CDS             complement(3378..4760)
                     /locus_tag="VGM24_11965"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156143.1"
                     /translation="MNKFWVILCFALAGLAFTTVIHRENGDEPVPVPPSKQRTGDTAA
                     GYKYLTTGDYLKSGIPLSYFKMAFGKTSANYLNRGGENAAVPYDYTVVKASNGEMVVA
                     PNCMQCHAQVFDGKFIMGLGNSSADFRFSQKLDPKNAAAAEKMLKNLDPHKYEAAEPF
                     LRVVKAVGSKLNTQVRGVNIADRLASLLASHRDRNTFKWSDEPLLDVPDEVIPTDVPA
                     WWLLWKKNAMFYNGFGRGDFGRFLMASNLFTVNDTSESREVDSHFNDVLAYIQSLRPP
                     AYPKPLDKALVKQGGVLFVQNCSKCHGSYGSGGKYPNLLIPESVIGTDSLLFKSNYQD
                     SQFIAWFNNSWFAKGDHPARLEPFDGYIAPPLDGVWITAPYLHNGSVPDLETLLDSKE
                     RPKYWSRDFDNPQYNYVDPGWKYQEHSAPEGTTVYNTTLPGYGNYGHTFGDRLSSRER
                     KALIEYLKTL"
     gene            complement(4828..>5773)
                     /locus_tag="VGM24_11970"
     CDS             complement(4828..>5773)
                     /locus_tag="VGM24_11970"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEY4156144.1"
                     /translation="EWKWDGIRGQIIRRNGQLFVWSRGEELMTEKFPELALLQQRLPE
                     GIALDGEIICLSPDRSDAVDFLPLPFAVLQTRIGRKNISKKQLTEAPVSFIAYDLLEY
                     EAEDFREHPQSERRAKLEELVKNIQLPVLRLSPLIPFHEWNDLAGIRTKSRETGSEGL
                     MLKRKEAVYQTGRKRGDWWKWKIDPLVIDAVMVYAQKGHGRRSNLYTDYTFAVRNGDQ
                     LVTFTKAYSGLTDKEFNEVDYFVKRNSLEKFGPVRTVKPELVFEIAFEGIAGSKRHKS
                     GVALRFPRINRWRKDKTPDEINTLEDLQQMLKLYGKGN"
BASE COUNT         1324 a         1595 c         1395 g         1459 t
ORIGIN      
        1 ctgcaggacc aggtgagcac attaaagaga aagatgaact aggaccggct attgaaaccg
       61 tacgatcgaa ttatttacga tcgcaaagac ctgctcctct tcttccggat cgatgagcgc
      121 cacgccggta aaccggctga aagcaggcag taccgcatat tccttcccga aataaaaaca
      181 gggaaaacag agcgactgtt tgcccatacc ctggatacgg atccccggat gaatatgccc
      241 ggaaaaaaaa tagggtcggg ccagtcggga atgaccggtg aacccttcat cctggtattg
      301 acagccgctg atatcatgca caaaatgaaa ccggttcatg gaaagggttg tccggttcag
      361 ccggatcccg gcattttcat accagtcttc tttcaatata tcgtgattcc ctttcaccag
      421 ttgaatatcc agatccggaa gatccgctct ccatttcaga aaaagttcca gttccagatt
      481 tgccctgctg tgaaacatat cgccgacaat gatcagttgc ctgggctgga aatgctggat
      541 ctggacgatc agccgctgaa gatcttcttt ataaacggat tggggaaccg cgatgcccga
      601 cttgcggaaa tgacctgttt ttccgaaatg cagatcggaa acgatcaggg ctttttcttc
      661 ttcccagaac agggtccggt cgcccgtgag ccagaactgc tgatttaaaa ttttatgggg
      721 aaacggagat tgcatgataa gctgcgaaga tattttattt tttccgttca ggaacgattc
      781 acacgttttc tttcgtggtc tttattgagt tgctcctgca ttttcttaat ccggtcctcc
      841 agtttctccg aagacaggtc ttcgcgcata ctgtccactt tcaaaggaaa acaaaatgga
      901 gtaagctttt ccggaaagct gatcacgatc cggctttctt tgatgcgctc cagcatattt
      961 ctcagacgca cttcttccat ctgctgttcc atgacttcct ggtaagcctg ccggagcaat
     1021 aaattgtgcg gatcatattc gctgaacaca ttgaacagca gtcccgcgga ggattgcagg
     1081 tgacgggcct ttttatgttc gccgggataa ccctgaaaga tcaggccgcc gatcacggct
     1141 atgtcgcgaa atttccgccg ggccatctcc gcgctgttta cgctgcgctg gatatccgca
     1201 ctgagctctt catccgaaaa cagatcatgc gcattggtat catcgagtgg aatgggctgg
     1261 tcgctcagca gttcaaagcc ataatcattc atggcaaaag aaaagctgat gggcactatg
     1321 cggctgatgc ggtaggccag cagggccgcc atcgcttcat gaacgagccg gccttcaaag
     1381 ggatagacaa acaaatggaa cccgtctgcg gtttcgatct gttcgatcag caattcatcg
     1441 tccttgggga tatgagaaag tttctgctgc agttcaaaca ggggtttcag ggaacgcaat
     1501 tcaatatccg gcaccgattt ctcccggcct tccgccacga gccaggcttc gttgaatttt
     1561 tttcgcagca tgcggccgag attggccgag agcggcattc gtcccccgtt ccagctgggc
     1621 acgattgctt ttttggcatt ggattttttt acaatggccg tcatttcccg gatcatcacg
     1681 aattccaggt tgcgcccggc cagcgtaaag acatcgccgg gatttaaacg ggatatgaaa
     1741 tattcttcaa tcactcccac atatccgcca ctcatgaatt tcactttaag catggcatcg
     1801 ctcacgatgg tgccgatatg caaacggtgc cgcatggcca cccgtcttcc ggtgatcttg
     1861 tatagtccgg ctatcacttc cacttttcgg aactcatcgt attgctgcag cgcttcgccg
     1921 ccctcggtga tgaagtgaag gatctggttc cattcttccc gcgtaatatc gcggaaacaa
     1981 taagtggaag aaatttcccg gaagatctgg tccggataaa atccctcccc gatggccagg
     2041 gtccccaggt actggatcaa cacgtcaaaa caaagcatca tcggttcccg gctttcaatg
     2101 aaattcagcc ccatcgccag tttcagcgca gccgcttcca ccagttctag ggaatgcgtg
     2161 ggcagaaaat agatcttgct gatctccccg ggctggtgac cgctgcgccc cgcgcgctgc
     2221 agaaagcggg cgaccccttt cggagaacca acctggatca cggtttccac gggccggaaa
     2281 tccacgccca gatccaggct cgccgtgcag acgacggctt tcagtttacc gctgtgcagc
     2341 gcctcttcca cccataaccg aagttcccgg tccacggaac catgatgcaa tgcgatcgct
     2401 ccggccaggc cgggagccac attcaggatc tcctggtacc agcgttcgct cattccccgc
     2461 gtattgataa agatgagggt ggttctgctc ttctcaatga tgggaatcac tttatgaacc
     2521 agtttgattc ccaggtgtcc cgcccaggga tatttttcaa tctcctccgg aatgacggat
     2581 tcgatttcaa tccgcttgtc aatgcttgct tttaccgtca ccggaggaaa atgcatttta
     2641 gccagcagcg gggaaagcaa tacttcccgg gcctgctcca ggttcccgat ggtggctgaa
     2701 ataccccaga cggaaaatgg aaatacctta ttccccgcga tctccacggg atccgccatt
     2761 ccaggaaaag gagcatggta agattcaatc agatgggcaa tccgtgaaac ggccagctcc
     2821 acctgcactc cccgtttgga tccgagcagc tcatgccact cgtccaccgc gaggatcttc
     2881 aatcctttga aaatttccgg gtaacctttt tgagccagca gcagatgcag gctctccggt
     2941 gttatgatca gcacctccgg catatgtctt ttctgttttt ccctttcact gatctccgtg
     3001 tcgccgttgc ggatcccgat cctccaggtc attcccaggc cggcgatcac ctccgtcatg
     3061 gcccggccga tgtcttttgc cagggcccgc aaaggagtta tccagagcag gcgcagctga
     3121 ttcggagacc gggttctata atcctccgga tgctcgttga tgaaatgaat gagagagccc
     3181 aggaaaacgg caaatgtttt tccgcatccg gtaggcgcat tcaccaaacc ggaatggcct
     3241 tccccgatat gttcccaggt ttcttcctgg aaagcaaaag gggtcagccc gctggattcg
     3301 aaccattcca ggatcacctg gtatcctttg gtctgttgta acgccatgaa ataaaatcta
     3361 atgctgccgg caggagctta cagtgttttt aaatactcga tgagggcttt tctttccctg
     3421 ctgctgagcc ggtctccaaa ggtatggccg taattgccat aacccggaag cgtggtatta
     3481 tatacggtgg tcccttccgg agcgctatgc tcctggtatt tccagcccgg gtctacataa
     3541 ttatactgcg ggttatcaaa atcgcgcgac cagtacttcg gtctttcctt gctgtccagg
     3601 agggtttcca ggtccggcac ggatccgtta tgcagataag gcgccgtgat ccagacccca
     3661 tccagcggcg gggcaatata tccgtcaaag ggttccagcc gggccggatg gtcccccttt
     3721 gcaaaccagg aattgttgaa ccaggcaatg aactgggaat cctggtaatt gctcttgaac
     3781 aacagggaat ccgttccgat cacggattcc ggtatcagca ggttgggata ttttcccccg
     3841 cttccgtaac tgccgtggca ttttgaacag ttctgcacaa acagcacccc tccctgtttg
     3901 accagcgcct tatccagtgg tttcggatag gccggcggcc gcagcgactg tatataggcc
     3961 agcacatcat tgaaatgact gtccacttcc ctggattccg aggtatcatt tacggtgaat
     4021 aaattggagg ccatcaggaa acgtccgaag tcgccccggc cgaatccgtt ataaaacatc
     4081 gcgttctttt tccacaataa ccaccaggcc ggcacatcgg tggggatcac ttcatccggc
     4141 acatccagca gcggttcatc gctccattta aacgtgttgc ggtcgcggtg actggccagg
     4201 agggacgcca ggcggtccgc aatattcact cccctcacct gcgtatttaa ttttgaaccc
     4261 acggctttca ccacccgtaa aaaaggttct gccgcttcat atttatgcgg atccagattt
     4321 tttaacattt tctctgccgc ggctgcgttt ttgggatcca gtttctggct gaaccggaaa
     4381 tccgcgctgc tgtttcccaa tcccatgatg aatttcccgt caaacacctg ggcatggcat
     4441 tgcatgcaat tcggcgctac caccatttcg ccgttggacg ctttcaccac cgtgtaatcg
     4501 tagggcaccg ccgcgttctc gccgccccgg ttcagataat ttgccgaggt tttcccaaaa
     4561 gccattttaa aataggataa ggggatcccg cttttcaaat aatcgccggt ggtcagatat
     4621 ttataccccg ctgccgtatc ccccgttctt tgtttgctgg gcggaaccgg aacgggttca
     4681 tcaccgtttt cccggtgaat gacggtagta aaagccaggc cggccaaagc aaaacaaagg
     4741 atcacccaaa atttattcat agtgaatttt cgctctaaaa ttaagcccga acaccgggtt
     4801 gaccaagtaa tggggatcag ggaaaagtta atttcccttt ccgtaaagtt tcagcatctg
     4861 ctgcaggtct tccagggtat tgatctcatc cggtgtttta tctttccgcc agcggttgat
     4921 ccggggaaac cggagggcta cgccggattt atgtcttttg gatcccgcga tcccttcgaa
     4981 tgcaatttca aaaacgagtt cgggtttcac cgtccgcacc gggccgaatt tttcaaggga
     5041 attccgtttc acaaaataat ccacttcgtt gaattcttta tccgtcaatc cggaataggc
     5101 tttggtaaaa gtgaccagct ggtccccgtt acgcacggca aacgtgtaat ccgtatacag
     5161 gttgcttctc cggccatgac ctttttgcgc atagaccata acggcatcga tcaccagcgg
     5221 atcaatcttc cacttccacc agtcaccgcg tttgcggccc gtctgataga ccgcctcctt
     5281 ccgtttcagc atcaatcctt cgctcccggt ctcccgcgat ttggtccgga tgccagccag
     5341 atcattccat tcatgaaaag ggatcaaagg agaaagacgc aaaaccggga gctgaatatt
     5401 ctttaccagc tcttccagct tcgctcttct ttcggattga ggatgttccc tgaaatcttc
     5461 tgcttcatat tccagcagat cataggcgat gaatgacacc ggagcttcgg tgagttgttt
     5521 tttgctgata ttcttcctcc cgatgcgcgt ctgtaatacg gcaaacggca gcggtaaaaa
     5581 atcaacggca tccgaccggt ccggcgacag gcagatgatc tctccgtcca gtgcgatgcc
     5641 ttcgggaagt ctttgctgaa ggagcgccaa ttccggaaac ttttccgtca taagctcttc
     5701 tccccggctc cagacgaaga gctgcccgtt gcggcggatg atctgtccgc ggatgccatc
     5761 ccatttccat tcc
//
LOCUS       DASYCU010000594         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4770238, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000594 DASYCU010000000
VERSION     DASYCU010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4770238"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VGM24_11975"
     CDS             <1..542
                     /locus_tag="VGM24_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY4156145.1"
                     /translation="LELAKTFLASGDFLWNAGIFVWQVKNVIRAFEKYMPEMYDVFIA
                     EKERFNTPEEKKALDYIYPLCTSISIDFGIMEKADNVYVIPSSFGWSDLGTWNSIYDN
                     IEKDFLGNAIAGENVMVIDARDSIVHTGDKKLIVLQGLENFIIVDTHDVLLICKRDHE
                     QEIKDYVAEVRRNRGDEYL"
     gene            599..1741
                     /locus_tag="VGM24_11980"
     CDS             599..1741
                     /locus_tag="VGM24_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HEY4156146.1"
                     /translation="MKFESKLPSTGTTVFTVMSALASKTRAINLGQGFPDFPMNQDLI
                     EKVYQAMREGFNQYAPMEGWMPLRETIAEKIEDLYQRPVRPDSEITITPGGTYAIYTA
                     LTTLLEPGDEVIVFEPAYDSYIPGIRLNGALPIAIPLEYPSYRINWEKVRAAVSPKTK
                     LIMLNSPHNPTGAVLSYEDIEELRKVVQGTNIFIHSDEVYEHIILDGLPHLSMLRYPD
                     LFERSFVSFSLGKVFHCTGWKMGYSVAPEWMMKEFRKIHQFNAFSCHTPVQVAMASFL
                     KEKSNYLSLPGFMQKKRDKFQELMTRTRFKPLQTHGSYFQIYSYAEINDSNDFDFAKQ
                     LVAHHGVAAIPVSAFYQSGKDDKVLRFCFAKKEETLEAAVAKLRKA"
     gene            complement(1761..4220)
                     /locus_tag="VGM24_11985"
     CDS             complement(1761..4220)
                     /locus_tag="VGM24_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4156147.1"
                     /translation="MLKNYLTIALRNFRHNKIFTIINVLGLSIGISSALVIFLIVHYD
                     FSFDKFEKDGNRVYRVVTDMKFAGTPYHNRGVPAPLPEAVKKEVPGVEEVIGFHQPNG
                     DLKVSIAKPGENKPAVFKDQSGIIYADKNYFSLIPFQWLAGSPKTALDGPYKVVLTEQ
                     RAKTYFSGIPAADMLGKQVVYDDSVIATVTGIVKDLDHNTDFSFKEFISQSTIVSAGI
                     KDHYGWEMWGSINSGSALFLKLDAGTPAKAVESRLLGLLKKYSKSGNPDERNTTVFHL
                     QPLNDLHFNAVYGTFGERTANKPTLYGLLIVAAFLLLLGCINFINLTTAQASQRAKEI
                     GIRKTMGSSRKLLILQFLNETFLISLAATLISILITPLLLTVFADFIPRDLHFDLLNQ
                     PYLILFLLLLVIGVSIFSGFYPALVLSGYKPVQVLKNQVHPSGASSRKALLRKSLTVS
                     QFFIAQVLVMATLITVKQIHFMLNKDMGFKKAAIITFVSPINWNNFSHPDPKRLVLLN
                     ELKSIPGIQMLSLASAPPSASGTTSSTMTYTDGKKEVTADVVHKSGDPDYIRLYQIKL
                     LAGRNIEPSDTTKEYLVNETYMHILGIQNPADILNKRINGLPVVGVMADFNQESLHKP
                     VKPMVFSADTRFTFIFNIALKPQDAQGILWSTTIGRIEKAYKNIYPEDDFQYAIFDQS
                     IAKFYKSEEDISRLLKWAAGLAIFISCIGLLGLVIYTTRLRTREIGIRKVLGASVSHL
                     LGILSRDFIGLVLIAFLIATPLAWLVMHKWLENFAYRTSISWWLFIASGGIMMLIALV
                     TLSIQTIKTANANPVESLRTE"
BASE COUNT         1148 a         1110 c         1136 g         1100 t
ORIGIN      
        1 acctcgaact ggcaaaaact ttcctggcca gcggcgattt tctctggaac gcgggcatct
       61 ttgtatggca ggtgaaaaat gtgatccggg ccttcgagaa atacatgccc gagatgtatg
      121 acgtgttcat tgcggaaaaa gaaagattca atacccccga agagaaaaaa gcactcgatt
      181 atatttaccc gctctgcact tccatttcca tcgacttcgg catcatggag aaagcggaca
      241 atgtctacgt cattccttct tccttcggct ggagcgatct cggtacctgg aacagcatct
      301 acgacaatat cgaaaaagac ttcctcggca atgccatcgc cggcgagaac gtgatggtca
      361 tcgacgcgcg cgacagcatc gttcacaccg gcgataaaaa gctcatcgtg ctccagggcc
      421 tcgaaaattt catcatcgta gacacccacg acgtgctgct catttgcaaa agagatcatg
      481 agcaggagat caaggattat gtggcggaag tgcggaggaa ccggggcgac gaatatctct
      541 agggtggaat gttttttgat ggaatccaac cgctgtccac catatcatta ttacattcat
      601 gaaattcgaa tccaaactac cttccaccgg cacaacggtt ttcaccgtaa tgagtgcatt
      661 ggcttcgaag accagagcga tcaatctcgg tcagggcttt ccggatttcc cgatgaacca
      721 ggacctgatc gaaaaagtgt accaggccat gcgggagggc ttcaaccagt atgcgccgat
      781 ggaaggctgg atgcccctgc gcgaaaccat cgcggagaaa atagaggatc tttatcagcg
      841 tccggttcgt ccggattcgg agatcacgat aacccctggc ggtacatacg ccatttacac
      901 ggcactcacc accctgctgg aacccggcga tgaagtgatt gtttttgaac ccgcttacga
      961 cagttatatc cctggcatcc ggctgaacgg ggccctgccc atcgccattc cgctggaata
     1021 tccttcttac cggatcaact gggagaaagt gcgggccgcg gttagtccca aaacaaaact
     1081 gatcatgctg aactcgccgc ataatccgac cggcgcagtg ctttcttatg aggacatcga
     1141 agaactgcgc aaggtcgtgc aggggaccaa tatttttatt cattccgatg aagtgtatga
     1201 gcacatcatc ctggacggat tgccccacct gtccatgctg cggtacccgg atctgtttga
     1261 aagaagtttt gtgagctttt ccctcgggaa agtttttcat tgcaccggct ggaaaatggg
     1321 ctacagcgtg gcgccggaat ggatgatgaa ggagttccgg aagatccacc agtttaacgc
     1381 cttcagctgt catacgcccg tccaggtggc catggcctcc tttctcaagg aaaaaagcaa
     1441 ctatctttcc cttccgggtt tcatgcaaaa aaagcgggat aaattccagg agctcatgac
     1501 ccggaccagg ttcaaaccgc tgcagaccca cggcagctat ttccagatct attcctacgc
     1561 ggagattaac gactcaaacg atttcgactt cgccaaacag ctggtcgccc accacggcgt
     1621 tgccgccatt cccgtctccg ccttctacca gtcaggcaag gacgataaag tgctgcgctt
     1681 ctgctttgct aaaaaggagg aaacgctgga agccgcggtg gcgaaactgc gcaaagcata
     1741 agaggtatac aattcaaccc tcattcagtc ctcagactct ccacgggatt tgcgttggcc
     1801 gtttttattg tctgaatgct cagcgtgacc agggcaataa gcatcatgat cccgccgctg
     1861 gcaataaaca gccaccagct gatggatgtg cgataggcaa aattttccag ccatttatgc
     1921 atgaccagcc aggccagggg cgtggcaatg agaaaagcga tcaggaccag cccgatgaaa
     1981 tccctggaca ggataccgag gagatgactg acggaggcgc cgagcacctt gcggatgccg
     2041 atctcccggg tgcgcagccg ggtggtatag atgaccagtc ccaggaggcc gatgcagctg
     2101 ataaatatgg cgagcccggc ggcccatttt aataaacggg aaatatcctc ttcgctctta
     2161 tagaatttcg cgatgctctg gtcgaagatt gcgtattgaa aatcgtcttc gggataaata
     2221 tttttatacg ctttttcgat ccgcccgatg gtcgttgacc acaggatacc ctgcgcgtcc
     2281 tgcggtttta aagcgatatt aaaaataaaa gtgaaccggg tgtccgcaga aaagaccatg
     2341 ggctttaccg gcttgtgcag ggattcctgg ttgaagtccg ccatcacgcc aaccaccgga
     2401 agtccgttga ttcgtttatt gaggatatcc gcgggattct gaatacctaa aatatgcata
     2461 taggtttcgt tgacgaggta ctctttggtc gtatcgctgg gctctatatt tcttccggcc
     2521 agtaatttga tctggtacag cctgatataa tcgggatcgc cggatttatg aaccacatcg
     2581 gcggtcactt ctttttttcc atccgtatag gtcatggtgg atgaggtggt cccggaagcg
     2641 gaaggcggcg cgctggccag gctcagcatc tgtatgccgg gaattgattt cagttcgttc
     2701 agcaatacca gccgctttgg gtcggggtgt gaaaaattat tccagttgat tggagagaca
     2761 aaggttataa tggccgcttt tttaaatccc atgtccttgt tcagcatgaa atgaatctgt
     2821 tttaccgtga tcagggtggc cataaccaaa acctgggcga taaaaaactg ggaaacggtc
     2881 aggctctttc tcagcaatgc cttccgggag ctggcgccgc tggggtggac ctggttcttt
     2941 aaaacctgca cgggtttata tcccgacagg accagggccg gataaaaacc ggagaagata
     3001 ctcaccccaa tcaccaacag cagcaggaat aggatcaggt aaggctggtt caacagatca
     3061 aaatgcagat cccttggaat aaaatcggca aaaacggtca gcagcaaagg cgtaatgagg
     3121 atcgagatca gggtcgccgc caggctgatc aaaaaagttt cattcaggaa ttgaaggata
     3181 agcagttttc tcgaactgcc catggttttg cgtataccga tctcctttgc ccgctgcgaa
     3241 gcctgggccg ttgtcaggtt gataaaattg atgcagccga gcaaaagtaa aaaggcggct
     3301 acgatcagta atccatataa ggtgggctta ttcgctgtgc gttctccgaa ggttccatag
     3361 accgcgttaa aatgcagatc gttaagcggc tgtaagtgaa agacagtggt attcctttcg
     3421 tcgggattgc ctgatttact atatttcttc aataacccca aaagccggga ttcgactgct
     3481 ttggccggag tcccggcatc gagctttaaa aagagtgcgg aaccgctgtt gatcgaaccc
     3541 cacatttccc atccgtaatg atcttttata ccggcgctga cgatggtgga ctgggaaatg
     3601 aattctttaa aactgaaatc cgtattgtgg tccaagtcct ttacgatgcc ggttacggtt
     3661 gctatgaccg aatcgtcgta aacaacctgc tttcccagca tgtccgcggc gggtattccg
     3721 gaaaaatagg tttttgccct ttgctcggtc aacaccactt tataaggtcc gtccagtgcc
     3781 gttttcggag atccggcgag ccattgaaaa gggatcaggc tgaaataatt tttgtccgca
     3841 taaataatac cagactggtc tttgaaaaca gccggtttgt tctcccccgg ttttgcaatg
     3901 gataccttga gatcgccgtt gggctgatgg aagccaatca cttcttccac acccggcact
     3961 tcctttttta ccgcttcggg aagcggcgcg gggactcctc tattgtggta gggcgttccg
     4021 gcaaatttca tatcggtcac cacccggtaa acccggttcc cgtctttttc gaatttgtca
     4081 aagctgaaat cataatgtac gatcaggaag atcactaagg ccgaactgat cccgatggat
     4141 aaacccagca cattgatgat cgtgaatatt ttattatgcc tgaaattcct cagggcaatg
     4201 gtcaaataat ttttgagcat ttgatcgggg gttcaagaaa aatgccaacc ctgaaaacca
     4261 gtatgaatca ggcggttaga aaaaaaataa aaaagatcac tgtgcggaac cgatacaatg
     4321 agtgtacggg gaccagccgt tgatggctgg tggatcttcc ctggtgcatc aactcctctt
     4381 tcgccgtttc aggtatttct gatgaatgat ttcgctcatc gtcttctcct gcaccttgct
     4441 ttcgagccag gaaatgatgt cgagataggc aaaggagcgc gtttcgaagc ggtt
//
LOCUS       DASYCU010000595         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3465417, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000595 DASYCU010000000
VERSION     DASYCU010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465417"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VGM24_11990"
     CDS             complement(<1..772)
                     /locus_tag="VGM24_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY4156148.1"
                     /translation="MNKIQLKTTYKKILSDIYTPVGIYLRLRDRFRDTVLLESTDYHA
                     AENSYSFICIHAIGGVEICDDRQMEFKYPGRPAEKIELKSAAVPALLWEYMQKFEVEP
                     ATEKPVKTAQSLFGYTTFDAVRFFDTINLPDSKEAPLIPLMRYRLYQYVIAINHFRDE
                     LYICENVLTGLESEAALVESLIRSKDVPVYPFRVNGCEKSNLTDSGYMEIVEKGIRSC
                     LRGDVFQIVLSRRFQLPFIGDEFNVYRALRNVNPSPYLF"
     gene            complement(980..2119)
                     /gene="hisB"
                     /locus_tag="VGM24_11995"
     CDS             complement(980..2119)
                     /gene="hisB"
                     /locus_tag="VGM24_11995"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HEY4156149.1"
                     /translation="MKRILFIDRDGTLIREAPPTYQVDDFSKLEFYPSMFEFMGRIAR
                     ELDYELVMVTNQDGLGTAAFPEEKFWPVQELVMKSLENEGIHFSAVHIDKSLPAEQSP
                     NRKPGTGMLTAYLNSEDYDLPGSYVIGDRITDVQLAKNLGCAAIWIKHDVSLGGREIR
                     DSISDLQKVVALETTRWEEIYGFLKSGLRQVVHERNTNETKIHIELNIDGSGKTDICT
                     GLGFFDHMLEQIARHGKMDLRILVEGDLQVDEHHTIEDTGIALGEAFGRALADKRGME
                     RYGFALPMDDAEALVLLDFGGRNWMVWNVSFQREKVGDMPTEMVFHFFKSFSDAVRCN
                     LNIECHGENEHHKIEAIFKAFAKAVRMAVKRDPLSNYLPTTKGIV"
     gene            complement(2200..>3061)
                     /locus_tag="VGM24_12000"
     CDS             complement(2200..>3061)
                     /locus_tag="VGM24_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4156150.1"
                     /translation="IVTGLLSRGEHEAKPIALDDEGNLFVTIGAFSNSCQIEDRKTGS
                     LGMKPCPVLDSAGGIWEFKADKPNQTYGDGIKYSTGIRNAMGIDWNTATHSLFVTQHG
                     RDQLSSIFPALFTTKQNAELPAECLYELHKGSDAGWPYIYYDQIQGKKMLAPEYGGDG
                     KKTGGENAIDPIVAFPGHIAPNDLLFYTGNQFPEKYKNGAFIAFHGSWNRAPEPQEGF
                     FVAFVPFSNGKPSGKWEIFANGFAGTPEDVASGKAIHRPCGLAQGPDGALYVTDDDKG
                     TIWKITYKAK"
BASE COUNT          710 a          818 c          707 g          826 t
ORIGIN      
        1 aaaataaata gggactcggg tttacattgc gcagggcgcg gtagacattg aattcatcac
       61 caatgaaagg cagttgaaag cggcggctca gcacgatctg gaatacatct ccccgcaggc
      121 agctccggat ccctttttca acgatctcca tataaccgga gtccgtcaga ttgctttttt
      181 cgcagccgtt tacgcgaaac ggatatacgg gtacatcctt gctgcggatc agggattcca
      241 cgagggccgc ttcgctctca agtcccgtca atacgttttc acagatatac aattcatccc
      301 tgaaatgatt gatcgcgata acgtactgat aaagccggta gcgcatcagc ggaatcaggg
      361 gagcttcttt tgaatccggc aggttgatcg tatcaaaaaa acggaccgca tcgaatgtcg
      421 tatacccaaa aaggctctgg gccgttttta ccggtttctc ggttgccggt tccacttcaa
      481 atttctgcat atactcccag agcaaagccg gaaccgcggc agatttcaat tcaatttttt
      541 cagcaggcct cccgggatat ttgaattcca tctggcggtc gtcgcaaatc tccacgcccc
      601 cgatggcatg gatgcagatg aatgaatagc tgttttccgc tgcatgataa tccgtgctct
      661 ccagcagtac ggtatcgcgg aaccggtcgc gcaggcggag ataaatgcct accggtgtgt
      721 agatgtcgga aaggatcttt ttgtatgtcg tctttaactg gatcttgttc ataaacctct
      781 gcttgatgat ttttataccc ggccccttca tccggcaaaa aaaaagcccc ggataaaacc
      841 ggggcctgtt atgtttacac acagctatcg cattacgcgc cccgttcgga gttcgtatgc
      901 caccaccatg tattattgat cagctttctt gtcatgcttt gcaaatatga ggcgtaattt
      961 tcagattacc aaatcgcgtt cagacaattc ctttggtcgt cggcagataa ttgctcaacg
     1021 gatcccgttt aacggccatc cggaccgctt tggcaaaggc tttgaaaatc gcttcaattt
     1081 tatgatgctc attctctcca tgacattcaa tatttagatt gcagcgtacc gcatcggaga
     1141 aggatttgaa aaaatgaaag accatttccg tcggcatgtc tcccactttt tctctttgga
     1201 aagaaacatt ccataccatc cagttccggc cgccgaaatc cagtaggacc agggcttccg
     1261 catcgtccat gggcagggca aagccgtatc tttccattcc ccgcttatct gccagggccc
     1321 tgccaaaggc ttcaccgagc gctatcccgg tatcttcaat ggtatggtgt tcatccacct
     1381 gcagatctcc ttccaccagg atcctgaggt ccattttccc gtgccgggca atctgctcca
     1441 gcatatgatc aaaaaaacca agccccgtac agatatcggt ctttccgctt ccgtcgatat
     1501 tcaactcaat atggattttc gtttcattgg tattgcgttc gtgaaccacc tggcgaagtc
     1561 cggatttcag aaacccgtaa atctcttccc accgggtggt ttccagggcg accactttct
     1621 gcaggtcgct gatcgaatcc cggatctccc gtcctcccag gctgacatca tgctttatcc
     1681 agatggccgc gcagcccaga tttttagcca gttgcacatc ggtgatgcgg tccccgatca
     1741 cataagatcc cggaagatca tagtcttcgc tgttcagata cgcggtgagc atgccggttc
     1801 ctggtttgcg gtttggagat tgttcggccg gcaatgattt gtctatatgc actgcgctga
     1861 aatgaattcc ttcattttcg aggcttttca taaccagttc ctggaccggc cagaattttt
     1921 cttccggaaa ggcggctgtt cccaggccgt cctggttggt aaccatgacc agttcatagt
     1981 ccagttcgcg ggctatcctg cccataaatt cgaacatgga cgggtaaaat tccagcttgc
     2041 tgaagtcatc cacctgatag gtgggcggtg cttcccggat cagcgttccg tcccggtcta
     2101 taaacagaat ccttttcaaa atttcaaaaa tttaaaccct gaaaatgaat ccggaaaacg
     2161 ctgcagggac cgacgcgcag gcatgctcct ccttcccggt cattttgctt tataagttat
     2221 tttccagatg gttcctttat cgtcatccgt tacatagagt gctccgtccg gaccctgggc
     2281 cagtccgcag gggcgatgaa ttgccttgcc ggatgccaca tcctccggcg tgccggcgaa
     2341 tccattggca aatatttccc attttccgga aggtttgccg ttgctgaatg gaacaaaagc
     2401 cacaaaaaat ccttcctgtg gttccggagc ccggttccag gatccgtgaa atgcgataaa
     2461 tgccccgttc ttatattttt ccggaaactg attcccggta taaaacagca aatcgttggg
     2521 ggcaatatga cccgggaagg ccacaatggg atcgattgca ttttccccgc ctgttttctt
     2581 tccatccccg ccatattccg gtgccagcat tttctttccc tggatctggt cgtagtagat
     2641 atagggccat ccggcatcgg atcccttatg caattcataa agacattctg ccggaagttc
     2701 ggcattttgt ttcgtcgtaa ataaggcagg gaaaatagaa cttaactgat cccggccatg
     2761 ctgcgttaca aacaaactgt gcgtggccgt attccaatcg atgcccatgg cgttgcgtat
     2821 cccggttgaa tactttatac catccccgta agtctggttg ggcttgtctg ctttaaattc
     2881 ccagatgcca ccggctgaat ccaataccgg gcagggtttc atacccaaag atccggtttt
     2941 ccggtcctcg atctgacagc tgttggagaa agcgccgatc gtgacaaaca gattaccttc
     3001 atcatccagg gcgatgggtt ttgcttcgtg ttcaccccgg gagagaagcc cggttacaat
     3061 t
//
LOCUS       DASYCU010000596         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5205433, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000596 DASYCU010000000
VERSION     DASYCU010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5205433"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VGM24_12005"
     CDS             <1..720
                     /locus_tag="VGM24_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEY4156151.1"
                     /translation="LVPAIYYNFFPAAGKTSFNDMTGSSWGRIVIAGVLVCLFGIYLC
                     GKAGMGKEKEVPDAEKKKSIAEFNLTKGLIVAIISGILSACFNFGIEAGKPMAYRAVA
                     LGSNPLYQNNVIYVVLLWGGLTTNFIWCFFLNLRNRSFGKYADKTTPLLKNYGLSALA
                     GTTWFLQFFFYGMGESKLGNGASSWILHMAFIILVANMWGLVLKEWKSVSRKTKTTII
                     AGIVTIILSVCIVGYGNALKQ"
     gene            754..>2381
                     /locus_tag="VGM24_12010"
     CDS             754..>2381
                     /locus_tag="VGM24_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEY4156152.1"
                     /translation="MSDTKQFKHVSYLWDEARAASFGGDEVALLIYRSNLLGADLRLT
                     NYGGGNTSCKIPAPDPLTGKETEVMYVKGSGGDLGTLRKSGLAALYMDRLLALKKIYR
                     GIEQEDEMVELFNHCIYDLHSKAPSIDTPLHAFLPFRHIDHLHPDAAIALAAAKDGKK
                     ITKEVFNGSIGWVEWQRPGFDLGLKLKACLDENPGIRGIMLGSHGLFTWGDTAYSSYI
                     NTLEVIEQCAAYIEKNLGKGKPVFGGVKINSLSPSERKEKAASLMPLLRGYASSHTRM
                     TGHFSDSDAVMEFINSNGLEKLAPMGTSCPDHFLRTKISPLVLPLSTSDDLGNIPAIR
                     EKLLPAFEAYRKMYREYYEQCKHADSPAMRDPNPVILLFPGVGMFSFAKDKSTARIAS
                     EFYTNAIQVMKGAEAISSYISLPRQEAFNIEYWLLEEAKLQRMPKPRPLTGKIALITG
                     SAGGIGKAIAKKFIQEGAAVFINDNDASRLESAREEFQQQFGKDSFESGILDVTDSLT
                     IQEAFKKTILAFGGVDIMVNCAGLSISKPLEEHT"
BASE COUNT          645 a          540 c          625 g          571 t
ORIGIN      
        1 ctcgtgcccg cgatttatta taattttttt cctgccgcag gaaaaacttc tttcaacgac
       61 atgaccggca gtagctgggg gaggatcgtg atcgccggtg tgctggtttg tcttttcggc
      121 atttatctct gcggaaaagc aggaatggga aaagagaaag aggtgccgga tgcggagaag
      181 aagaagtcca ttgcggaatt caacctgacc aaaggactca tcgtagccat catttccggg
      241 atcctcagcg cctgttttaa ttttgggatt gaagccggca agccaatggc ctaccgggcc
      301 gtagcgctgg gatcgaaccc gctctatcag aacaatgtga tctatgtagt gctcttatgg
      361 ggcgggctga ctactaattt tatctggtgt tttttcctga atctccggaa ccgcagtttt
      421 ggaaaatatg cagataaaac gactccactg ctgaagaatt acggattatc cgcgctcgcg
      481 ggaaccacct ggttcctgca attctttttt tatggaatgg gagaaagcaa attgggaaac
      541 ggggccagct cctggatcct tcatatggct tttatcattc tcgtagcgaa tatgtgggga
      601 ctggtactta aagaatggaa atcggtgagc aggaaaacaa aaacaaccat cattgccgga
      661 attgtgacca ttattctctc ggtttgtata gtggggtatg gcaatgcatt gaaacagtga
      721 ccaattgaat tactaaaaaa taaagggatc gacatgtccg atacaaaaca gttcaaacac
      781 gtaagttatc tatgggatga ggccagggca gcctcctttg gcggcgatga ggtcgctttg
      841 ctgatttatc gctccaacct gctgggcgca gacctgcggc tcaccaatta cggaggcgga
      901 aacacatcgt gtaaaattcc agcccccgac ccgcttaccg ggaaagaaac cgaagtgatg
      961 tatgtgaaag gttcaggggg agatctggga acgctccgga aaagcggact ggccgctttg
     1021 tatatggacc gcctgctggc attgaaaaaa atataccggg gcattgaaca ggaagatgaa
     1081 atggtggaat tgttcaatca ttgcatttac gatctgcatt ccaaggcgcc gtccatcgat
     1141 acgccattgc atgcttttct tccgttccgc catattgacc acctgcatcc cgacgccgcc
     1201 attgccctgg ctgcggcgaa ggacggtaaa aagatcacga aggaggtttt taacggatcc
     1261 attggctggg tggaatggca aagaccgggg ttcgacctgg gtttaaaact gaaggcctgc
     1321 ctggatgaaa atcccggaat ccgcgggatc atgctgggat cacacggatt gttcacctgg
     1381 ggagatacgg cttacagcag ctatatcaat acactggaag tgattgaaca gtgcgcagcc
     1441 tatattgaaa aaaatctggg aaaaggaaaa ccggttttcg gaggcgttaa aataaattct
     1501 ttatcccctt cggaaagaaa ggaaaaagcg gcctcgctga tgcctttact gagagggtat
     1561 gcttcctccc atacgcggat gaccggccat ttctccgatt cggatgccgt gatggaattc
     1621 ataaattcaa atggactgga aaaactggcg cctatgggaa ccagctgtcc ggatcatttt
     1681 ttaagaacaa agatcagccc cctggtgctc cccctctcca cttcagacga cctcggaaat
     1741 attccggcga tacgggagaa actgctcccg gcatttgagg cctaccggaa aatgtaccgc
     1801 gaatattatg aacagtgcaa acacgccgat agtcccgcga tgcgcgatcc gaatccggtt
     1861 atattgctct ttcccggagt cggtatgttc agttttgcaa aagataaatc cacggcaaga
     1921 attgcttccg agttttatac caatgcgatc caggtgatga aaggagcgga ggccatttca
     1981 tcctatattt cgcttccccg gcaggaagcc ttcaatattg aatactggtt gctggaggaa
     2041 gcgaaattac agcggatgcc caaacccagg ccgcttacgg gaaagatcgc cctcatcacc
     2101 ggaagtgccg gaggaatcgg aaaggccatt gcaaagaaat tcattcagga aggtgcggca
     2161 gtatttataa atgataatga tgccagccgg ctcgaatctg cgcgggagga atttcaacag
     2221 cagttcggga aagacagttt tgaatccggg atcctggatg tgacagacag cctcaccatt
     2281 caggaggctt ttaaaaagac catattggca ttcggaggcg tggatattat ggtgaactgc
     2341 gcgggccttt ccatttcgaa accattggaa gaacatacgg a
//
LOCUS       DASYCU010000597         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_275217, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000597 DASYCU010000000
VERSION     DASYCU010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275217"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VGM24_12015"
     CDS             complement(<1..796)
                     /locus_tag="VGM24_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156153.1"
                     /translation="MACYKRVIFFFIGLTGLSAYGQTVARLDSISQHFIESRRSEAGE
                     KIFLQTDKWYYAAGEDIWFRAYCMNAISHKPSRHSKTLFVDLVNDRDSPVCQLLLNNQ
                     ELQPGGRISLPSFLPEGYYWLRAYTRDILRDDSNRIYVQPVYLLSRNKPGSSRLTGTP
                     ESPAAPVSESGPPQLIFYPEGGAIVQGTNTTVGIYAQDKSGKPLTVSGNMADGRGIVL
                     SGFKTSFQGLGKSVFFATKSGVYTAQLRDSGNTGINYPLPRTDPFSS"
     gene            complement(839..2101)
                     /locus_tag="VGM24_12020"
     CDS             complement(839..2101)
                     /locus_tag="VGM24_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEY4156154.1"
                     /translation="MQRFQGLIGVFLILGLAFLFSNNRRRINGRLVASGLFLQLMIAV
                     LVLKVGPVTRFFQFLGRGMERLEHFAFQGASFVFSGVAVPTPGGGAKDYAMGGFVFAF
                     NVTATIILVCALVALLYHVGLMQKIVALIAKAMNFIMRVSGAEALSNVASAFVGQIEA
                     QIMIQPYLPGMTKSELLASMSGSLACIAGGILIVYVNMGAQAGMDLAPKLIAASLMAA
                     PGALVISKIVWPETEESQTLGAVKLKVASRYTNLLDAITHGAGEGFKIAMNVIAMLIG
                     FIAMIALVDYLLKKTGHLFNPSFDLSLNWIFGKIFYPFAWSMGVPEQDIGNVATLLGQ
                     KLTVNEFVAFKNLTSGAVPVLSQKGLMIVSIAICGFANFSSVGMQIGGIGAIVPERRA
                     DLARLGMKALLCGTLASYLSATIAGILI"
BASE COUNT          590 a          626 c          604 g          543 t
ORIGIN      
        1 gactggaaaa cggatccgtc cggggaagcg gataatttat tccggtgttg ccggaatccc
       61 ggagctgagc ggtatagacg cctgatttcg tcgcaaaaaa aaccgatttt cccaatccct
      121 ggaaggatgt tttgaatccg gaaagaacaa ttccccttcc atcggccata tttccggata
      181 ccgtcagcgg ctttcccgat ttgtcctgcg cataaatccc caccgtcgta ttcgttccct
      241 gcactatggc gccgccttcc gggtaaaata tcaattgcgg tgggcctgat tccgaaacag
      301 gcgccgccgg gctttccggc gttccggtta accgggagct tcccggttta ttccggctca
      361 gcaggtaaac cggctgaacg tagatccgat tgctgtcgtc ccgcaatata tcgcgcgtat
      421 atgccctgag ccaataatac ccttccggta aaaaggaagg cagggagatc ctccccccgg
      481 gctgcaactc ctgattgttc aggagcaatt ggcataccgg actgtcccgg tcattgacca
      541 ggtccacaaa aagggtttta ctatggcggg aaggtttatg agaaatggca ttcatacaat
      601 aggccctgaa ccagatgtcc tcgcccgcgg cataatacca tttgtccgtt tgaaggaaaa
      661 tcttttctcc ggcttccgat ctccggcttt cgataaaatg ctgtgaaatg gaatccagcc
      721 gggcaactgt ttggccgtat gcggaaagac ccgttaaccc gatgaaaaaa aatattaccc
      781 gtttataaca ggccatatcg tttgttttag atgccgtcct aacggcaaac aaattaattc
      841 atatcaggat tccggctatc gttgccgaaa gataggatgc cagggtgccg cagagcaatg
      901 ctttcatgcc cagccgcgcg agatcggcgc gtctttcggg aacgatggcg ccgataccgc
      961 ctatctgcat gcccacgctg ctgaaattgg caaagccgca gatcgcgata ctgacgatca
     1021 tcaatccttt ctgggaaagg accggcacgg cgccggaggt gaggttctta aacgcgacga
     1081 actcgttgac cgttagtttt tgtccgagca gggtggccac attcccgata tcctgttccg
     1141 gcacgcccat cgaccaggcg aagggataaa atatttttcc gaagatccag ttcagactca
     1201 ggtcaaaaga aggattgaac agatgaccgg ttttcttcag cagataatcc accagggcga
     1261 tcatggcgat aaacccgatc agcatggcta tcacgttcat ggctattttg aatccttctc
     1321 ccgcgccatg ggtaatggca tcgaggagat tggtgtaccg gctggccact ttcagtttta
     1381 ccgcgcccag cgtctggctt tcttctgttt ccggccaaac gatcttggaa atgaccagtg
     1441 caccgggagc cgccatcagg ctggcggcaa tgagcttggg cgccagatcc atgccggcct
     1501 gtgcacccat gttcacgtaa acgatcagga tgccgccggc aatgcaggcc aggctgccgg
     1561 acatactcgc cagcaattcg cttttggtca ttcccggcaa ataaggctgg atcatgatct
     1621 gcgcttcaat ttgccctacg aatgcgctgg ccacattact cagcgcttcc gccccgctca
     1681 cccgcatgat gaaattcatc gcctttgcaa tcagggccac gatcttttgc ataagtccca
     1741 catggtataa aagagcgacc agcgcacaga ccagtatgat cgtggccgtt acattgaacg
     1801 cgaatacaaa accgcccatc gcatagtcct ttgctccccc gcccggagtg ggaacggcaa
     1861 cgcctgaaaa aacaaacgag gcgccctgaa aggcaaaatg ttcaagcctt tccattccgc
     1921 ggccgaggaa ctgaaagaac ctggttacgg gccccacttt caataccagc acggcaatca
     1981 tcagttgcag gaatagtccg ctggccacca ggcggccgtt gatgcgccgg cggttgttcg
     2041 agaaaagaaa tgccagtccg agtatcagaa aaaccccgat cagtccctga aatctttgca
     2101 tgaaccccgg tttattttat cagggaagat aaaggaatta aacaagaaac ggaatacgga
     2161 tgccgcatcc cgtacctgat caaactggat tttccttcgt aattttgtgc ctcatttaaa
     2221 acaggttgga atggatgctg gtaatcggga aatgtatcgg ttggtggaga atcagtccca
     2281 cgcggggctt cggaaaatcg gggaaaaaat agtagcgggg aaaagaattg attttgatga
     2341 aggggtgctg ttgtttgaaa aag
//
LOCUS       DASYCU010000598         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1580497, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000598 DASYCU010000000
VERSION     DASYCU010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580497"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VGM24_12025"
     CDS             <1..1609
                     /locus_tag="VGM24_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY4156155.1"
                     /translation="IHLRSKMDYEAEPNGDITRVYVFSLIGFFILLIACINYMNLSTA
                     RSALRAREIGIRKVIGARKKGLIIQFLSESVLISWVALVIAVLLTAFLLPWLNKLSGQ
                     SISFDILLKWQVLIPLLLMPFVIGFVSGIYPALFMSSFQPVKTLKGLFKMGGNGISFR
                     KALVVIQFAISIMLIITTAIVFQQLHYMQNKSLGFDKEQILILPYGTDINKRYDAFRT
                     AILQNAAFREVGRSSRIPTGRLLDASSASTLVGDSLRPVTTDIKMVNVDYDFIPTYGI
                     RLLAGRNFSREFGTDTAGFVLNESAIKAVGWKSPRDAVGKEFKYGRTDGRVIGVINDF
                     NFESMHQKIIPLVLIMTAPSMGYYGNISIKLRGRNIYAALDYLEKTWKTLLPETAYQY
                     SFLDDNFDKLYQSEQRQGTLFILFACIAIFIACLGLFGLSAFAISQRVKEIGVRKVLG
                     ARVSRIVALLATDFLRLVVIAAFLAFPIAWYAMHNWLKDFAYRVSIQWWIFVLAGVLA
                     ALIAFLTVSFQAVKAANANPVKSLRTE"
     gene            1745..>1994
                     /locus_tag="VGM24_12030"
     CDS             1745..>1994
                     /locus_tag="VGM24_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156156.1"
                     /translation="MNYHYGRIRLNEAIIAIQLGNIRKAENLLAETEEKIKKWDDLMG
                     NLAMAYLLLHEESKAVAVIRTALSQNSKWEEHLPSFFLL"
BASE COUNT          479 a          479 c          487 g          549 t
ORIGIN      
        1 tatccacctg cgttccaaaa tggattatga agcggagccc aacggggaca tcacccgtgt
       61 atatgttttt tccctgattg gtttttttat tctcctgatt gcctgtatca attacatgaa
      121 cctatccact gcccggtctg cgcttcgggc ccgtgaaatc gggatccgca aagtaatcgg
      181 agcaagaaag aaagggctga tcatccagtt tctcagtgag tcggtactga tcagctgggt
      241 tgccctggtc atcgctgtgc tgctgaccgc ctttttgctg ccctggctta ataaactatc
      301 cgggcagtca atttccttcg acatattatt aaaatggcag gtgctgattc ccctgctgct
      361 catgcctttt gtcatcggtt ttgtctcggg gatttatccg gctctcttca tgtcctcctt
      421 tcagccggta aaaacactga aaggcctgtt taaaatgggg ggcaacggca tttctttcag
      481 gaaggccctg gtcgtcatcc agttcgccat aagcatcatg ctcatcatca cgacggcgat
      541 cgtatttcag cagttgcatt acatgcagaa caaatccctg ggctttgata aggaacagat
      601 cctgatcctc ccttacggaa ccgatatcaa caaacgatac gatgccttcc gcaccgccat
      661 tttacaaaat gccgccttca gggaagtcgg acgttcgtcg cgcataccca ccggcagatt
      721 attggatgct tcaagcgcgt ctacgctggt tggcgattcc ctgcgtccgg taacgacgga
      781 catcaaaatg gtgaatgtgg attacgattt cattcccact tatggtatcc ggctattggc
      841 cggaaggaat ttttcccggg aattcgggac cgatactgcc ggttttgtgc tgaacgaatc
      901 ggccataaaa gcggttggct ggaaaagccc ccgggatgcg gtcggaaagg aattcaaata
      961 tggccgcacg gacggccgtg tcataggcgt gatcaatgat ttcaatttcg aatccatgca
     1021 tcagaaaatc attccgctgg tgctgatcat gaccgcaccg tcgatggggt attacggtaa
     1081 tatttccata aaactgcgtg gccggaatat ttacgctgcc ctggattatc tggaaaagac
     1141 ctggaaaacg cttttgcccg aaacagctta tcagtacagc ttcctcgatg ataattttga
     1201 taagctgtac cagagtgaac aaagacaggg caccctcttc atcctgttcg cctgcatcgc
     1261 catatttatc gcctgtcttg gtctgtttgg attgtctgca tttgccatca gccagcgggt
     1321 caaggaaatc ggtgtgcgga aagtattggg tgcgcgtgtc agcaggatcg tggcccttct
     1381 ggcgacggat tttttaaggt tggtggtcat cgcggccttc ctcgctttcc cgatagcctg
     1441 gtatgcaatg cacaactggc tgaaggattt tgcctaccgc gtcagcattc aatggtggat
     1501 ctttgtgctg gcgggcgttt tagccgcgct gatcgctttt cttacggtaa gctttcaggc
     1561 cgttaaagcc gccaatgcca atccggtaaa aagcctgaga accgaatagg gggttatttc
     1621 agaaacactt taacatatta ttgagcctgt tgtccggatt tgtgtgcaac aaattaccgg
     1681 atgccgcgtc ttctttacct agtcagactc attttcttta ttctgtcctg ttaatcacga
     1741 aacaatgaat tatcactacg gaagaatccg gctgaacgag gccattatcg ccattcagct
     1801 ggggaatatc aggaaagctg aaaatttact ggcggaaacg gaagaaaaaa taaaaaaatg
     1861 ggacgatctg atgggtaatc tggcaatggc ttacctgttg ttgcacgagg aatcaaaagc
     1921 tgtggccgtg atccggactg ccttaagtca aaattcaaaa tgggaagagc atttaccttc
     1981 atttttcctg ctta
//
LOCUS       DASYCU010000599         7369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4481019, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000599 DASYCU010000000
VERSION     DASYCU010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4481019"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1382
                     /locus_tag="VGM24_12035"
     CDS             219..1382
                     /locus_tag="VGM24_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4156157.1"
                     /translation="MDLSYIINELGEDREHYFSAVAPPIIQTSNFRIEKVEELKKLFE
                     DEYSGFLYSRGLNPTVEILRKKLAALDQAEDCLVFNSGIAAIFAAVLANIKAGDHIIS
                     VEKPYSWVQKIFNHILSRFGVETTYIDGRRFENFELARRSNTRFIYLESPNSWTYAIQ
                     DIEAVAAFAKKHGIVTLMDNSYCTPLYQLPVPMGIDLCMQTATKYIGGHSDTLGGILT
                     GSGKGIKKIFDCEYMAVGSGIQPFNAWLLIRGLRTLESRISRITENTRKILDWLKRNS
                     RVDGVLFPLDPDFPQYELARKQMKNACGLISFYVKAGSRLQIVQFCENLKHIMMAVSW
                     GGHESLILPWCASLEDQEFDPGIPEHRMLRLYVGLENPEYIIRDLDQSFQLSF"
     gene            1503..2846
                     /locus_tag="VGM24_12040"
     CDS             1503..2846
                     /locus_tag="VGM24_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY4156158.1"
                     /translation="MTKTKKLVLFLCSVIFYLSAAAQDKWDLRRCVEYAVVNNISIKQ
                     ADIDSRVSKLAYDQAKWSQFGNVSFNSALGLTFGRSIDRTTNIYVNTQSLFHQYNLNA
                     GISLFNWFALRRAEESNKFSYQAQVVNIDKIKNDITLNVAAAYLQALLSREQVTLAKT
                     KLELTRQQLENTRKLVDAGSVPELNAAELEVQFATDTSAVISAQETYDINNLQLKAIL
                     NLDAAAPFDLDTPPVENIPVEPIAGLQPDIVYAVAVGTFPQQKMNDLRIRAAQKNVDY
                     NRGLLYPSLSLFAGLGDNFANSLQPFGKVFNGYGNQLSNNFGQNVGLQLTVPIFNGNL
                     SRTNYKRAQLGVITAQLTKENDLLTLKQNIYQSYYNAVASLQKYDANQKAVTVAEKSF
                     DLSTKRYNIGMLNTIDYLTNQNNLFSARINALIAHYDYVFRMKVLEYYKGLGIKL"
     gene            2869..4251
                     /locus_tag="VGM24_12045"
     CDS             2869..4251
                     /locus_tag="VGM24_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY4156159.1"
                     /translation="MNKKLLWIIISLVVIVVLLVILKKSGVIGKEEGVPVSMEKVEYR
                     SITETVTASGKVYPEIEVKMSSDVSGEIVELNVQEGDSVHKGQQLAKIYADIYGNQKN
                     QAAAQVSQQEAVVSNYTAQLPGLKATMDAAQAQFDRQKQLLAQKVISQSEFETAETTL
                     RTAQANYNAALQNVQSNKAAVASAKANLAIQAENVSRTTITSPISGVISLLSVKKGER
                     VVGNSMMAGTEMMRVADMSKIEAIVDVGENDIPKVLLGDSATVEVDAYNNRKFTGVVT
                     QIASSIVATSASSTSSSGSSTNDVTNYKVHIRLDPKSYKDLIDPKRPKSLVFRPGMTA
                     SADIQTKTHPRVLSVPINAVTTREKTAAAKPAAKAKEANDPEDQANKVIGSGGDLDEV
                     VFLLQPDKSVKQVSVKTSIQDINYIEIVQGLKEGDQVITGPYGTVSKVLGNGTKVKVV
                     PKEQLFETKK"
     gene            4294..5331
                     /locus_tag="VGM24_12050"
     CDS             4294..5331
                     /locus_tag="VGM24_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY4156160.1"
                     /translation="MRFGILGSGSWATAIAKILTDSHHSLNWWIRNEAVIQHLEKHRH
                     NPQYLSSVYFDTGKLKLSPKLGQVIADSGCIIVAMPSAYIEKIFGELPPDTFEGKQVL
                     SAVKGILPGKDLLLNEYLKNQFGFGTDQYFTLMGPCHAEEIASEKLSYLTFSGLSETS
                     TAELASHFTTDYLNTITNTDIYGVQYASVLKNIYALGAGMAHGLEYGDNFLSVFIANC
                     ADEMAGFLHKAGILHIEVGIHENSKPESVKKSANYAASVYLGDLLVTCYSLFSRNRTF
                     GNMIGKGYSVQAAKLELNMVAEGYYGSSCIYRINRQMKADMPVAETVYKILWENLPVR
                     EGFKRMENLLV"
     gene            complement(5497..6624)
                     /gene="hemW"
                     /locus_tag="VGM24_12055"
     CDS             complement(5497..6624)
                     /gene="hemW"
                     /locus_tag="VGM24_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY4156161.1"
                     /translation="MAGIYIHIPFCKQACHYCNFHFSTSLALKNDFTAALLKEIGLRS
                     DYLQNEIIRTIYFGGGTPSLLEESEMSAILQSLYRNFQVDQEAEVTLEANPDDVGAAA
                     LNGWKRAGINRLSLGVQSFFREDLIWMNRAHDEKQALRSVLDAQAAGLDNISIDLIYG
                     LPTLTDEKWKENTATAVSLGIPHLSCYALTVEPKTALFRRIELGKMQDTVPEDQARQF
                     LEGIQWLEESGYEHYEISNFAKPGKRSRHNSAYWLSEKYLGLGPSAHSFNGKKRQWNI
                     ASNTRYIRSLAANELSFETETLRPVDQLNEYIMTSLRTLEGLDLRQVNREFGSEKAEQ
                     LEKNTARYIAAAQMKKENEKLFLTPEGKLFADGIASGLFFD"
     gene            6672..>7369
                     /locus_tag="VGM24_12060"
     CDS             6672..>7369
                     /locus_tag="VGM24_12060"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY4156162.1"
                     /translation="MVKLRLYGCTVLILLISAFNGYSQYALRVSMQSPDSILSAKALG
                     IPASFKDRNECTDYVYKLPGILQDKGFASASVDSVHFDSASAFVHLYVGDRFSWSAIR
                     IRPEDAALLRAAGWSTKKLSGKPARLDRLQGEEVLLLDYLENNGYPFARISMDSVRFE
                     RGSLSAVVKIEKGPLYRIDSIRVHGTARISSSFLERYLGIPEGSIYRKDKLEAISKRI
                     LELPYVQEEHPWNVT"
BASE COUNT         1968 a         1776 c         1828 g         1797 t
ORIGIN      
        1 tcagtggttt tataggcgga agaaaacgat tattgtgatt gcccaccgcc tgtctgtcgt
       61 aaaacgctgc caccggatca tcctgatgaa ggaagggaag cccgtggccg atggaacgca
      121 tgaacaattg atcagggaaa atgaagaata tgccggctgg tgggagaata attagattat
      181 ctaacttgct ccttcaggtc ttaaattgcc cgattaccat ggatctttca tacatcataa
      241 atgaattagg ggaagacaga gagcattatt tcagtgcggt ggctccgccc attatccaaa
      301 ccagcaattt ccggatagaa aaggtggagg aactgaagaa gttgtttgaa gacgagtaca
      361 gcggcttcct ctatagccgg ggactcaacc ctacggtgga aatcctgaga aaaaagctcg
      421 ccgcactgga ccaggctgag gactgcctgg tcttcaacag cggcattgcc gctatttttg
      481 cggctgttct ggctaatatc aaagcaggcg accatatcat ctcggtggaa aaaccatatt
      541 cctgggtaca aaagatcttt aatcatatcc tgtcgcgctt cggagtggaa acaacttata
      601 tcgacggcag gcgcttcgaa aattttgaac tggcccgacg gagcaatacc cggttcattt
      661 acctggaaag tcccaattcc tggacttatg ccatacagga tattgaagca gtggccgcct
      721 ttgcgaaaaa acacgggatc gtcacgctga tggataacag ctattgcacc cccctgtacc
      781 agttgccggt cccgatggga atagaccttt gcatgcaaac ggcaaccaag tatatcggcg
      841 ggcacagcga caccctggga gggatcctga ccggatccgg aaaagggata aaaaagatat
      901 ttgattgcga atatatggcc gtcggaagcg gcatccagcc gtttaatgcc tggctgttga
      961 tccggggact gcggaccctg gaatcccgga tttcgagaat cacggaaaac acccggaaga
     1021 tattggattg gcttaaaagg aactcgcggg tagacggggt cttattcccg cttgacccgg
     1081 atttcccgca atatgaactg gccaggaaac aaatgaaaaa tgcctgcgga ttaattagtt
     1141 tttatgtgaa agcgggttcc cgcctccaga tcgtgcaatt ctgcgaaaac ctgaaacata
     1201 tcatgatggc tgtcagctgg ggcggtcatg aatccctgat ccttccatgg tgtgcttccc
     1261 tggaagatca ggaatttgac ccgggtattc cggaacaccg gatgctgaga ctgtatgtcg
     1321 ggttggaaaa cccggaatat atcatcaggg atctggacca gtctttccag ctttcgttct
     1381 aaaaggattt taagcatttt ttaatggaga atgagaatgg attctttact ttagcaatat
     1441 ttctgacaaa cccccttatc cggtaagctg tgaaggggat gggttcccaa taaacttctt
     1501 tcatgaccaa gaccaaaaaa ctagtcttat tcctatgttc cgtcatcttc tatttaagcg
     1561 ctgctgccca ggataaatgg gacctgcggc gctgcgtgga atatgccgtc gtaaataata
     1621 tttccatcaa acaggccgat atcgattccc gggtatcgaa actcgcttac gaccaggcaa
     1681 aatggagcca gtttggaaac gtcagtttca atagtgccct gggcctgacc tttggccggt
     1741 caattgaccg gaccaccaat atatatgtga atacgcagag ccttttccat cagtataacc
     1801 tgaatgccgg tatttccctg tttaactggt ttgccttacg ccgcgccgag gaatcaaata
     1861 aattcagcta ccaggcccag gtggtgaata ttgataaaat aaaaaatgat attaccctga
     1921 atgtggcggc cgcctatctt caggccctgc tgtccaggga acaggttacc ctggcaaaaa
     1981 ccaaactgga attaacccgt cagcaactgg aaaatacccg caaactcgta gatgcgggat
     2041 cggttccgga actgaatgcc gcggagctgg aagtacaatt tgccacggat acctcagccg
     2101 taatctctgc tcaggaaacc tacgatatca ataatctcca gttaaaggcc attctgaatc
     2161 tggatgccgc tgcgcccttt gacctggaca cacctcctgt ggaaaacatc ccggtagaac
     2221 ccatcgccgg tcttcagccc gatatcgtgt atgccgttgc cgttggaact ttcccgcaac
     2281 aaaaaatgaa cgacctgcga atccgtgctg cgcagaaaaa cgtggactat aaccggggcc
     2341 ttctttatcc gtcgctttcg ctttttgccg gcctgggcga taattttgcg aatagcctcc
     2401 agcctttcgg taaggtattc aacggatatg gaaaccagct gagcaataat tttggccaga
     2461 atgtgggttt gcagctcacc gttcccatct tcaatggaaa tttatcccgc accaattata
     2521 aaagggcgca attgggagtg attaccgccc aactgaccaa ggagaatgac ctgctcacgc
     2581 tgaagcagaa tatttaccaa tcttattata atgcagtggc ttcgcttcag aaatacgatg
     2641 ccaaccaaaa ggcggtcacc gttgctgaaa agtcttttga cctgtctacc aaacggtaca
     2701 atatcggcat gctgaacacc atcgattacc tcaccaatca gaataacctg ttttccgccc
     2761 gtattaatgc acttattgcc cattacgact atgtattccg gatgaaagtg ctcgaatact
     2821 ataaggggct tgggattaaa ttataaaaaa aacttacaac ctgatcgtat gaataagaaa
     2881 ctgctttgga ttataattag tctggtcgta attgtggtcc tcctggtcat tttgaaaaaa
     2941 agcggcgtga tcggcaagga ggaaggggtt ccggtttcca tggaaaaggt ggaataccgc
     3001 tccattacgg aaaccgttac cgccagcgga aaggtctatc ccgaaattga agtgaaaatg
     3061 agttctgacg tttccggcga aatcgtcgaa ctcaacgtgc aggaaggaga tagtgtccac
     3121 aaaggccagc agttggctaa aatttatgcc gatatctatg gaaatcaaaa gaatcaggct
     3181 gctgcccagg taagccagca ggaagccgtg gtatcgaatt acaccgccca gctgccgggg
     3241 ttaaaggcaa ccatggatgc cgctcaggcg cagttcgacc ggcagaaaca actgcttgcc
     3301 caaaaagtga tctcccagtc ggaatttgag acggcggaaa ccacgctgag aaccgcccag
     3361 gcaaattata atgcagccct tcaaaacgtg cagagcaata aggcggcagt ggccagcgca
     3421 aaggcaaacc tggccatcca ggcagaaaac gtgagccgta ccaccattac ttcccccatt
     3481 tccggcgtga tctcgctgct cagtgtcaaa aaaggcgagc gcgtggtggg aaattccatg
     3541 atggcaggaa cagaaatgat gcgcgtggca gatatgagca aaattgaagc gatcgtggac
     3601 gtgggggaaa acgatatccc caaagtgctg ctcggtgatt cggccaccgt ggaagtggat
     3661 gcctataaca accggaaatt cactggggtt gtcacccaga tagccagcag tatcgtggcc
     3721 acctccgcct cctctacctc cagcagcggc agcagcacaa atgacgtgac caactacaaa
     3781 gtacatatcc ggctggatcc gaaatcctac aaggacctga tagatcccaa gagacctaaa
     3841 agcctggtct ttcgtccggg aatgaccgcc agtgcggata ttcaaacgaa aacacatccc
     3901 cgggtgctgt ccgttccgat caatgcggta actacccggg agaaaacggc tgctgcgaag
     3961 ccggccgcca aagccaaaga agcaaacgat ccggaagatc aggccaacaa ggtaattggt
     4021 tccggtggag atctggacga agtggttttt ctgcttcagc cggataaatc cgttaaacag
     4081 gtgagcgtga aaacgtctat ccaggacatc aactatatag aaattgttca gggtctgaaa
     4141 gaaggggacc aggtgatcac gggcccttat ggtacagtga gcaaggtact cggtaacgga
     4201 acaaaagtta aggtagtgcc aaaggagcaa ctcttcgaaa ctaaaaaatg accccgttca
     4261 aaggctgtat ctttacgcta aattcaagcg tagatgcgtt ttggaatcct tggaagcggg
     4321 agctgggcaa ccgctattgc aaaaatcctt accgacagcc atcattccct gaattggtgg
     4381 atcaggaatg aagccgtcat tcagcatctc gaaaaacatc ggcacaatcc ccaatacctt
     4441 agttcggtat atttcgatac cggtaaactg aaactcagcc caaagcttgg ccaggtgatc
     4501 gcggactccg ggtgcattat cgtggcaatg ccctccgcat atattgaaaa aatctttggg
     4561 gagcttcccc cggacacttt tgaaggtaag caggttcttt cggccgtcaa gggcattctt
     4621 cccgggaaag acctgctgct caatgaatat ctcaagaacc aattcgggtt cggaacagat
     4681 cagtatttca ccctgatggg cccctgtcat gcagaggaaa ttgcttcaga gaaattgtct
     4741 tatctcactt tttcgggact ttcggaaacc tccacggcag aactggcgtc tcatttcaca
     4801 acggattatc tgaataccat tacgaatacg gatatctatg gcgttcagta tgcatcggtg
     4861 ttaaaaaata tttatgcgct tggagccggc atggcgcatg gcctggaata cggggataat
     4921 tttttaagcg tatttattgc caattgtgcg gatgagatgg cgggatttct tcataaggcc
     4981 ggaatcctgc atattgaggt ggggatccat gaaaacagca agccggaatc ggtaaagaaa
     5041 agtgccaatt atgcagcatc cgtatacctg ggcgatctgc tggtaacctg ctattctctt
     5101 ttcagccgga accgtacgtt tgggaatatg atcgggaaag gatattccgt tcaggcagca
     5161 aagcttgaac tcaatatggt ggcagaagga tattatggaa gcagttgcat ttaccggatc
     5221 aaccggcaaa tgaaagcgga tatgccggtc gccgaaacgg tttataaaat cctgtgggag
     5281 aatttaccgg tcagggaagg ttttaaacga atggagaatt tgctggtctg atatataaag
     5341 gaaattttat gatgccgctt tcgtttcagg gttttggatt taccgcgatt cttattatgg
     5401 tggcagtcct ctccctttct cacggggtaa gctacccgag agagagattc cgggattcgc
     5461 ggcataaggc cgatggctga tggccccggc agattttcag tcaaaaaaca aacccgaagc
     5521 gatcccgtcg gcaaacaatt ttccttcagg cgtaagaaat aatttttcat tttctttttt
     5581 catctgggcc gcggcgatat aacgcgctgt atttttttca agctgctccg ctttctctga
     5641 accaaattcc cggttcacct gccggagatc aagcccttcc aatgttcgga gggaagtcat
     5701 aatatattca ttcagctgat cgacgggtct cagggtctcc gtttcaaaac tcagttcatt
     5761 ggccgcaagg gaccggatat agcgggtatt gctggctata ttccattgtc ttttttttcc
     5821 attgaatgaa tgggcggaag gacccagccc gaggtatttt tccgataacc agtaagcgct
     5881 gttatgccgg cttctttttc cgggtttggc aaaatttgaa atttcataat gctcgtaacc
     5941 gctttcctcc agccactgaa ttccctccag gaactgccgg gcctggtctt cgggaacggt
     6001 atcctgcatt ttacccagtt caattctcct gaacaaggcc gttttgggtt ctaccgtaag
     6061 ggcataacag gaaagatgcg gaatgccgag ggaaacggcg gtcgcggtat tctctttcca
     6121 tttttcatcc gttaacgtgg gcaggccgta gatcaggtct atgctgatat tgtccagccc
     6181 ggctgcctgg gcgtccaaaa ccgagcgtag cgcctgcttt tcgtcatgcg cccggttcat
     6241 ccagatcaga tcttcccgga aaaaggactg gacgcccagg ctcaggcgat tgattcccgc
     6301 ccgcttccag ccgttcagcg ccgccgcgcc gacatcgtcc ggattagcct ccagcgtcac
     6361 ttcagcctcc tgatctacct ggaaattccg gtaaagactt tgcagaatgg cgctcatctc
     6421 cgattcctcc agcaaagacg gcgtgccccc gccgaaataa atggttctga ttatttcgtt
     6481 ttgcaggtaa tccgatctca aaccgatttc tttcaataaa gccgctgtaa aatcgttttt
     6541 caaagccagg gaagtggaga aatgaaaatt acaataatga caggcctgtt tgcaaaaagg
     6601 gatatgaata taaatacctg ccatgccggt tcgctaattt tatcgaatat tatgatgatg
     6661 aaggagcacg aatggtaaaa ttaagactat atggctgcac ggtcctgatc ctgctgatat
     6721 ccgcctttaa cggatattcc caatacgcat tacgggtaag catgcagtcg cctgattcca
     6781 tcctgtctgc aaaagccctg ggtattccgg cttcctttaa ggaccggaat gaatgcacgg
     6841 actatgtgta caaacttccc gggatccttc aggacaaggg gtttgcctcc gcttcggtcg
     6901 acagcgtgca tttcgattcc gcgtctgcct ttgttcatct gtacgtaggg gaccgcttct
     6961 cctggtctgc catcaggatc cggcccgaag acgctgcctt attgcgtgcg gcgggatgga
     7021 gtaccaaaaa gctcagcgga aaacccgccc gcctggacag gttacaggga gaggaagtgc
     7081 tgctgctcga ttatctggaa aataatggtt atccttttgc ccgcatttcc atggatagtg
     7141 tccgctttga gcgtggatcc ctgagcgcgg tggtaaaaat cgagaaaggg cctttgtacc
     7201 ggatcgacag catccgggtt cacggaacgg cgcggatctc ctcttccttt ttggaaagat
     7261 atctcggtat cccggaaggg agcatatacc ggaaggataa actggaagcg atcagtaagc
     7321 gcatcctcga actgccctat gttcaggaag agcatccctg gaatgttac
//
LOCUS       DASYCU010000600         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3176893, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000600 DASYCU010000000
VERSION     DASYCU010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176893"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VGM24_12065"
     CDS             complement(<1..1164)
                     /locus_tag="VGM24_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY4156163.1"
                     /translation="MAQRSKSKKNQSSGPASLKTEKEAKLTVSQVIRDERTHKITGAV
                     FLLFSIFLFIAFTSFLFTWEEDQSIVRGISVFSASQNDRVANLLGSVGAHVSYFFFFN
                     GFGVASYFICSFFFITGVNWLFAKKIFSIRRNMRYVIIGLLFLSAALAFITRGSSFPW
                     GGAVGNIMSDWMIHFLGRIGTACVLFAGALSYFIWRFNPEFKVPAIPKKDRPAGPLPL
                     NEEEGGPSLFIEEPAPKNKKNKFGKEADIPLIFPDDEQQPDMAPFINEEFKPKMPVPP
                     VIPPRLPKKEPVTNPLELEIRAVPEKEPDEILTEGTPDKLPDYEPTLDLKSYKYPTLD
                     LLGAHGSDRIIQDPAELEANKNQIIGTLKNYDIDIQKISATVGPTVTLYEIVPS"
     gene            complement(1233..1628)
                     /locus_tag="VGM24_12070"
     CDS             complement(1233..1628)
                     /locus_tag="VGM24_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEY4156164.1"
                     /translation="MNKLFLTFAAVAGGIAVLLGALAAHQLREHMSAQALEVFETAVR
                     YQFYHVFALLAAGILSERFPGKWMNRSGSCFIGGILLFSGSLYIISALMAAGTTVPVG
                     LGILTPLGGLLFILGWIFLSTGLMKGRSS"
     gene            complement(1711..2214)
                     /locus_tag="VGM24_12075"
     CDS             complement(1711..2214)
                     /locus_tag="VGM24_12075"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY4156165.1"
                     /translation="MRIFLIGFMGSGKTYWGTRIAEALKIPFYDLDAVIVNGEGMPVT
                     EIFKEKGEEYFRYKEKQVLEELVAKNKSFVLSAGGGTPCFFNNIKFMKKHGKVIWLNT
                     STDLLKHRLMKERLTRPLLRDISEAGLHTYIVRKLGERKIYYEQADVTVLEDNILLDP
                     LISLLQN"
     gene            complement(2268..>3460)
                     /locus_tag="VGM24_12080"
     CDS             complement(2268..>3460)
                     /locus_tag="VGM24_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY4156166.1"
                     /translation="LPMAARTDAFQSPDYFELDELLTDEHRLIRESIRAYVKKEISPI
                     IEDYAQRAEFPKHIVPQLGALGCFGPTIPEVYGGGGLDYISYGLMMQELERGDSGVRS
                     TASVQGSLVMFPIHAYATEEMKKKMLPRLASGEWLGCFGLTEPDHGSDPGSMLSNIKD
                     AGDHVILNGSKMWISNAPFAQVAVVWAKDETGDIRGMLVERGMEGFSTPVTHGKWSLR
                     ASATGELVFDQVKVPKANLFPDIRGLKGPLSCLTKARYGIAWGSVGAAMDCYDTALRY
                     SKERVQFDRPIGGFQLQQKKLAEMITEITKAQLMNWRLGVLMNANRATPAQVSMAKRN
                     GCEIAANIAREARQVLGGMGITGEYSVMRHMMNMESVLTYEGTHDVHLLITGMDVTGL
                     NAFK"
BASE COUNT          884 a          933 c          777 g          866 t
ORIGIN      
        1 cgaagggacg atttcataca aggtgaccgt tggcccaacg gtggcgctga tcttctgaat
       61 atcaatatca taattcttca gggtgccgat gatctggttt ttattagctt ccagttctgc
      121 cggatcctga atgatccggt cgctcccatg cgctcccagc aaatccagcg tgggatattt
      181 atatgatttt aaatccaggg tgggttcgta atccggcaat ttgtccgggg ttccttcggt
      241 aagtatttca tccggttcct tttcaggcac cgcccggatc tccaattcca gcggattggt
      301 cacgggttcc tttttgggta accggggcgg gataacggga ggtaccggca tcttcggttt
      361 gaattcctca tttataaaag gggccatgtc cggctgctgt tcatcgtccg ggaaaatcag
      421 cggtatgtct gcttctttac cgaacttatt ctttttattt tttggcgcgg gctcttcaat
      481 aaacaacgag ggaccgcctt cctcttcatt caacggtaaa ggacctgccg gcctatcttt
      541 ttttggtatc gccggcacct tgaattcggg attaaatctc cagataaaat aagacagcgc
      601 cccggcaaaa agcacacagg ccgtaccgat cctgcccaaa aaatggatca tccaatcgct
      661 cattatattt ccaaccgccc cgccccaggg aaaggaggat cctctggtta taaaggcaag
      721 cgcagcagaa agaaacagga gaccgatgat cacatacctc atgttcctgc ggatagagaa
      781 tattttttta gcaaatagcc agttaactcc ggtaatgaaa aaaaagctgc agataaaata
      841 actggccacg ccgaagccat taaaaaagaa aaagtaagaa acatgggctc ccacgctgcc
      901 cagcaggttg gccaccctat cgttttggga tgcggaaaaa acggaaatcc cgcgaacaat
      961 cgactggtct tcctcccagg taaacaaaaa ggaagtaaat gcaatgaaaa gaaaaatgga
     1021 gaataataaa aaaacagccc ctgtgatctt atgtgtccgt tcgtcccgga taacctggga
     1081 aacagtcagc ttggcttcct tttcagtttt cagggaggct ggtccggagg attggttttt
     1141 cttcgattta gaacgctgag ccatggcaac aaagataaga aacccgaatg catcttttat
     1201 tccttaattc cctgatgccg gcctctgccg gctcaggagg aacgcccttt catcagcccg
     1261 gtgctcagaa aaatccatcc gagaataaat aacaggccac ccagcggagt aaggattccc
     1321 aagcccaccg ggacggtcgt tccggcagcc atcagcgcgg aaattatata aagggaaccg
     1381 gaaaaaagaa ggattccgcc aatgaaacag ctcccggaac ggttcatcca ttttcccggg
     1441 aaacgttcac ttaatattcc ggcggccagc agggcaaaca catgataaaa ctggtatctc
     1501 acggcggttt caaaaacctc cagggcctgg gcagacatat gctcgcggag ctgatgggcg
     1561 gccagcgcac ccagcagaac ggctatgccg ccggccaccg cagcaaaagt taaaaatagt
     1621 ttattcattt tcttatcatt cggaaagaga caggtaaaaa attgaagata cgcttcggcc
     1681 ggattaatcc gcgcaccctg ctctttaatt ttaattctgc aataaactga tcagcggatc
     1741 aaggagaata ttatcttcca ggaccgttac atccgcctgt tcataatata ttttccgttc
     1801 tcccagtttt cgaacgatat aggtatgcag gccggcttcc gaaatatccc gcaggagggg
     1861 acgtgtcaaa cgttccttca ttaaccggtg cttaagtaaa tcggtcgacg tattcagcca
     1921 gatcactttg ccgtgcttct tcatgaattt tatattattg aagaagcagg gcgttcctcc
     1981 tcctgcagaa agaacaaaac ttttattttt agccaccagc tcttcgagca cctgtttttc
     2041 cttgtaccgg aaatactctt cccctttttc tttaaaaatt tctgttaccg gcatcccttc
     2101 cccattcaca atcacggcat ccagatcgta aaaggggatt ttgagggctt ccgcaatacg
     2161 ggttccccaa tacgttttgc ccgaacccat gaagccaatg agaaatatgc gcatgtcaat
     2221 taagcaattt tgaaatccga taaccagcgt ctgccaaaga aaattattta tttaaaggca
     2281 ttcaaccccg tcacatccat tcccgtaatc agcaaatgga catcatgcgt tccttcatag
     2341 gtgagcacac tttccatatt catcatgtgg cgcatgacgg aatattcccc ggtgatgccc
     2401 attccgccca ggacctgacg ggcctcccgg gcaatatttg cggcaatttc gcatccattc
     2461 cgtttggcca tggaaacctg tgccggcgtt gcccggttcg cattcatcaa tacccccagc
     2521 cgccagttca ttaactgggc cttggtgatt tcggtgatca tctctgccaa ttttttttgc
     2581 tgaagctgaa acccgccgat cggccggtca aactgcaccc gctctttgct gtaccggaga
     2641 gcggtatcat aacaatccat ggcagcgccg acagatcccc aggcgattcc ataccgtgct
     2701 ttggtaagac aactcagcgg gcctttcaat ccgcggatat ccggaaaaag attggctttc
     2761 ggaactttta cctgatcaaa taccagctct cccgtggccg aagcccggag actccattta
     2821 ccatgcgtaa caggcgtact gaacccttcc ataccccgct ccaccagcat cccccggatg
     2881 tcaccggttt cgtcttttgc ccataccacg gcaacctggg caaacggcgc attgctgatc
     2941 cacatcttgc tcccgttcag gatcacatgg tctcccgcat cttttatatt gctgagcata
     3001 ctacccggat ccgatccgtg gtcgggctcc gtcagaccga aacagcccag ccattcaccc
     3061 gaagccaggc gcggaagcat cttctttttc atttcttccg ttgcataggc atggatcggg
     3121 aacattacga gggaaccctg gaccgaagcg gttgagcgaa ccccgctgtc tcccctttcc
     3181 aattcctgca tcatgagccc gtagctgata tagtccagcc ccccgccacc ataaacttcg
     3241 ggaatggtgg gaccaaaaca gcccagcgcg cccagttgtg gaacaatatg cttggggaat
     3301 tcagcccgct gggcgtagtc ttcgatgatc ggactgatct ctttttttac ataggccctt
     3361 atggactccc ggatcagcct gtgctcatct gtcagcaatt cgtccagctc aaaatagtca
     3421 ggactttgga aagcatctgt tcttgcagcc atgggtaaat
//
LOCUS       DASYCU010000601         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2304509, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000601 DASYCU010000000
VERSION     DASYCU010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304509"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VGM24_12085"
     CDS             complement(<1..753)
                     /locus_tag="VGM24_12085"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4156167.1"
                     /translation="MSGLNKWFSYFVLLLLLFSMQEAVAQQKLLRGVILDAQSGERVP
                     FASMRFLQSGNGKLSDSAGNFVFRFENWPKDTLEITYVGFQNYHLVLSDSLFRRYRKD
                     SISVVIQLQRGRYNNEVVVSRKVDFGLLLWRKIVRRKKFNDRYRFDNFSYELYNKLEL
                     DLNKVNKDKLESIRLLRPFDFVLNNIDSSEDHPFLPIYVTETLSDYYYQRSPLKRREV
                     ILGSKTVGLDNESVSKFLGGMDQNIDFYNNFIP"
     gene            848..2266
                     /locus_tag="VGM24_12090"
     CDS             848..2266
                     /locus_tag="VGM24_12090"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY4156168.1"
                     /translation="MQAIISHCKEYGFIFPSSEIYDGLSAVYDYGQNGSELKKNIRDY
                     WWKSMTQLQDNIVGIDAAIFMHPTTWKASGHVDNFSDPMIDNKDSQKRYRVDHLIEGY
                     ADELEAAGKPGEADELRAALDRLLAAGDFDGLKKLIMEKNIRCGISGTANWSEVRQFN
                     LMFSTQLGSVSEEASEIYLRPETAQGIFVNFQNVQKTGRLKIPFGIAQVGKAFRNEIV
                     ARQFIFRMREFEQMEMQFFIKPGTQKEWYEFWKKERMRWHLSLGIPAGKYRFHDHVKL
                     AHYADAATDIEYDFPMGFKEVEGIHSRTDFDLKNHQEFSRKKLAYFDTELNQHYIPYV
                     IETSIGLDRTVLMILSEAYAEEDLSTAEKKDSRVLLKLPPKFSPVKLAILPLVKKDGL
                     PEIAREILKTCRPFFRCFYEEKDTIGKRYRRMDAIGTPFCVTVDHQSREDQTVTIRYR
                     DSMEQERVPISGVRKIVEKEIL"
     gene            2407..2754
                     /locus_tag="VGM24_12095"
     CDS             2407..2754
                     /locus_tag="VGM24_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156169.1"
                     /translation="MLTNEVTLNIYLLAFVVFLAALAGFGLRATRLIKSRAKIEELER
                     EILNNYERILELERETFNMEAQLQDIKSPVIAMKTPAAKKEEMRASQKFPDISLRKQL
                     LGKKNIQQSASGL"
     gene            complement(2779..>3484)
                     /locus_tag="VGM24_12100"
     CDS             complement(2779..>3484)
                     /locus_tag="VGM24_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY4156170.1"
                     /translation="FWIELDSGKPFDFAPGQFVTLDLPVHEKPNKRWRSYSVASWPDG
                     SAIFELLIVYLEGGLGTNYLFNEVRLGSELVFRGPLGLFTLPDPIEKDLFMVCTGTGI
                     APFRSMIHHILNHNIAHQDIHLIFGCRRLSDSLYQKEMEELQEQVPRFQYIPVFSREV
                     PENQNHLIRTGYVHPVYEEICKKNSSAEFPQEVPVILKPAHFYLCGWKNMIDEAKKRI
                     QALGYDRKAIHLELYG"
BASE COUNT          960 a          840 c          865 g          819 t
ORIGIN      
        1 cggaatgaaa ttgttgtaga agtcaatgtt ctgatccatt ccacccagga acttgctgac
       61 gctctcattg tccaggccca cggttttact gcccaggatc acttccctcc gcttcagcgg
      121 ggagcgctga taatagtaat cggaaagggt ttcggtaacg tagatcggca ggaagggatg
      181 gtcctccgaa ctgtcaatat tattcagcac aaaatcgaag gggcgaagaa gccggatact
      241 ttccagctta tccttattca ccttgttgag gtcgagctcg agtttattat acagctcata
      301 ggaaaaatta tcgaagcggt accggtcgtt gaattttttc cggcgaacga tctttctcca
      361 taatagcagg ccgaaatcca ccttccgact caccaccacc tcgttattgt accttcctct
      421 ttgcaactgg atgaccacgg aaatactgtc tttccggtac ctccggaaca gggaatcgga
      481 aagaacgagg tggtagttct gaaagccgac ataggtgatc tccagggtat ctttgggcca
      541 gttttcaaaa cggaaaacaa aattccccgc tgaatcactc agctttccgt tcccggactg
      601 caggaaacgc atggaggcaa agggaacgcg ctctccgctc tgggcatcca gaataacccc
      661 ccggagtagt ttttgctggg caaccgcttc ctgcatggag aacaggagaa ggaggagtac
      721 gaaataagaa aaccatttat tcaatccgga cataccccca aaaatactac aatgcggcct
      781 aaggagttgc ttcctcatct tcattctcct tacttttgca gccattatgg ctaatgatgt
      841 tcaaaaattg caggcaatca tatcccactg caaggaatac ggttttattt ttccatccag
      901 cgaaatctac gacgggctca gtgccgtgta tgactatggc cagaatggca gcgaattgaa
      961 aaagaatatc cgggactact ggtggaaaag catgacccag ctccaggata atattgttgg
     1021 cattgatgcg gctattttta tgcatcccac cacctggaag gcctcgggac atgtggataa
     1081 tttcagcgat ccgatgattg ataacaaaga cagtcagaag cgctatcggg tggatcacct
     1141 catagaagga tatgccgatg aactggaagc cgccggaaaa ccaggcgaag cggacgaact
     1201 gcgggcggcc ttggaccgcc tgctggcagc cggcgatttc gacggactga aaaagctgat
     1261 catggagaaa aacatccggt gcggcatcag cggaacggcc aactggagcg aggtacgcca
     1321 gttcaacctc atgttctcca cccagctggg gagcgtatcg gaagaagcca gcgagattta
     1381 cctccggccg gagaccgcac agggcatatt tgtcaatttt cagaacgtac agaaaacggg
     1441 ccgtctgaaa attccctttg gtattgccca ggtgggaaaa gcgttccgta atgagatcgt
     1501 ggccaggcaa tttattttcc gcatgcgtga gtttgaacag atggaaatgc agtttttcat
     1561 taaacccggc acccagaagg aatggtatga gttctggaag aaagagcgca tgcggtggca
     1621 tctcagcctg ggtatcccgg caggcaaata ccggtttcat gaccacgtga agctggccca
     1681 ctacgcggat gccgccaccg acattgaata tgactttccg atgggtttta aagaagtgga
     1741 gggtatccat tcccggaccg atttcgattt gaagaaccac caggaattca gccggaaaaa
     1801 actggcttat ttcgataccg aactgaacca gcattatatt ccatatgtga tcgaaacctc
     1861 cattgggctg gatcgcaccg tgctgatgat cctcagcgag gcctatgccg aagaagacct
     1921 gagcacggcg gagaaaaaag acagccgggt gctgctgaaa cttcccccga aattctcgcc
     1981 ggtcaaactg gccattctgc cgctcgtaaa aaaagacggc ttgccggaga tcgcccgtga
     2041 aatcctgaaa acctgcagac catttttccg ctgcttttat gaggaaaagg acacgatcgg
     2101 aaagcggtat cgccggatgg atgccatcgg gacccccttc tgtgttaccg tagatcatca
     2161 gagccgggag gatcagacgg tgaccatccg gtatcgcgac agtatggagc aggaacgagt
     2221 gcccatttcg ggggtgcgta aaattgtgga aaaagaaatt ttataaaatt tttatttcaa
     2281 agggatgcct tacatttggg catcacctcc aattgatgat aaaccccgtt ggctgataaa
     2341 gaagatcctg accccagcgt gtacctgttg aagatctatt gactgattct ttaattaatg
     2401 aatgccatgc ttaccaacga ggttacactc aatatttacc tgcttgcttt cgttgttttt
     2461 ttagctgccc ttgccggatt tggtttaaga gcaacacggc tcataaaaag ccgcgctaaa
     2521 atcgaagaac tcgaaagaga gatcctgaat aattacgaac ggatcctgga gctggagcgc
     2581 gaaaccttca atatggaagc ccaattgcag gatattaaaa gtccggtgat cgccatgaaa
     2641 acgcctgctg caaaaaaaga agaaatgaga gcctcccaga aatttcccga tatctccctc
     2701 cgcaaacaac tgctcggcaa aaagaatatt cagcaatcgg cctccgggct ctgaggccgg
     2761 acaaccgatc gcattccgct aaccatacag ctcaagatga atggcctttc tgtcgtaacc
     2821 aagcgcctga atgcgttttt tggcttcatc gatcatattc ttccagccgc ataaataaaa
     2881 atgggccggt tttaggatga ccggaacttc ttgcgggaat tcagcggaag aatttttttt
     2941 gcagatctct tcataaacag gatgaacgta accggtccgg atcaggtgat tctgattttc
     3001 cggcacttcg cgcgagaaaa cgggaatgta ttgaaaacgg ggaacctgct cctgaagttc
     3061 ttccatttct ttctggtaaa gggaatcgct cagtctccgg cagccaaaaa tcaggtgaat
     3121 gtcctggtgt gcgatattgt gattcagaat gtgatgaatc atggaacgga acggggcgat
     3181 tcctgtaccg gtgcaaacca tgaacaggtc tttttcaatc ggatccggga gggtaaacag
     3241 acccaggggt ccccggaata ccagttcgct gcccagcctg acttcattga aaagataatt
     3301 ggtgcccagg ccaccctcca ggtaaacaat gagtaattca aatatggcgg acccgtcggg
     3361 ccaggaggca acggaataac ttctccagcg tttatttggc ttttcgtgaa cgggaagatc
     3421 caatgttaca aactgaccgg gagcaaaatc aaacggcttt ccggaatcca gttcgatcca
     3481 gaat
//
LOCUS       DASYCU010000602         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3900092, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000602 DASYCU010000000
VERSION     DASYCU010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3900092"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(552..642)
                     /locus_tag="VGM24_12105"
     tRNA            complement(552..642)
                     /locus_tag="VGM24_12105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(606..608),aa:Ser,seq:gct)
     gene            817..972
                     /locus_tag="VGM24_12110"
     CDS             817..972
                     /locus_tag="VGM24_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156171.1"
                     /translation="MKKLFTAVLQRTQPVSKPLLLVSFGAGYALATLLFIFYGNQIHR
                     LLESLFS"
     gene            complement(1115..>2343)
                     /gene="dnaA"
                     /locus_tag="VGM24_12115"
     CDS             complement(1115..>2343)
                     /gene="dnaA"
                     /locus_tag="VGM24_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY4156172.1"
                     /translation="ILGKEARLEYRIMVDGGNKQNKPLTMDVPAHSYKTVSNNEMDFP
                     LIINNPVKNPFVIPGLKKMQIDAQLNPVYTFDAFIEGDCNRVARRAGKTVAEKPGANS
                     FNPLVIYGGVGLGKTHLAQAIGNEVRRTHHNKVVLYVSSEKFINQFIDHSRNNAVNDF
                     IHFYQLVDVLIIDDVQFFNRAERSQDAFFAIFNHLHQSGKQLILTSDKPPKDLEGVQE
                     RLLSRFRWGLSADLQIPDYETRIEILERKMKNDGLEMPKEVVKYLAYNINSNVRELEG
                     ALISLLAQSSLNKREIDLELSKKVLRNFVKTSSKEITIETIQRMVCEYFDLPYDKLLQ
                     KTRKREIVQARQITMFLAKAFTKNSLKTIGEHFGGRDHTTVIHSCQTVKDLMDTDSMF
                     RENVTELQQKVQLAAM"
BASE COUNT          542 a          609 c          521 g          671 t
ORIGIN      
        1 tctcccattt tttcagttcg gctgcccttt cagcagcagt gagcggcttt ggggttgctt
       61 tttttgaatt ttcatttttg atttcgcgat tagcgaatac caggctgcct accacaacca
      121 gcggtaagat cagcacataa ataatttttt tcatatcctt ttaaagttga tgcaacaaaa
      181 atactttgat atatttcagc gcaggcaact tgcattgtaa atagaaatgt aacctttgta
      241 aatatattgt aaagggtagg tttgacagcc ggaatttact tttcggggaa cgcaatacct
      301 gttgcagatt ggttctaccc gtgtttatct ctttaacagg cgtcgctttc ggtacgaccg
      361 gcagtgacgg gagggaggga ttattccgcg ccctggcggg cggctccatg atcccgggtg
      421 ggggaacctg cagaaaagat gaagtgccgg cgctacgcgc ctgtgccttt ttcgttcctc
      481 cgctgcggcg aacccgttcg cctcgcgccg gaacgaaaaa tgctcccttc aggagcactt
      541 catcttttct gcggagaggg agggattcga accctcggta caagtttaag ctcgtacaac
      601 ggtttagcaa accggccctt tcggccactc aggcacctct ccggacactc ccctttttcc
      661 gggagcaccc gtcttttccg ggctggcaaa aataaagatt aattggaagc cggccttatt
      721 tttatccggg ccggtaaaga aaaacaggcc gggttaaaca atatttcaac cccgatatca
      781 gttttaatct gatccaatta tccgaataaa cccgagatga aaaaattatt taccgctgtc
      841 ctccaacgca cacagcccgt atccaaaccc ctgcttttag tcagttttgg cgccggttat
      901 gcgctggcca ccctcctttt cattttttac ggaaatcaga tccatcgcct gctcgagtcc
      961 ctgtttagct gacggatccc ggctgcttcg gtctgcggcc aaattcatcc cttagccccg
     1021 gtctgaaccc ggcagtttcc aggttttcct gcataaaaaa aacccaccgg gataccagtg
     1081 ggcaatacgt tttaggtttt gatcaacccc ggccttacat cgcggccaat tgcacctttt
     1141 gttgcaattc cgtgacattt tcccggaaca tgctgtccgt atccatcagg tcctttacgg
     1201 tctggcagga atgaatgacc gtggtatggt cccttccgcc aaaatgctct ccaatggttt
     1261 ttaacgaatt cttggtaaat gccttggcta aaaacatcgt aatctgcctg gcctgcacaa
     1321 tttcccgttt ccgggtcttt tgaaggagct tgtcgtaagg caggtcaaaa tactcgcata
     1381 ccatccgctg tatggtttca atcgtaattt ccttactact ggtctttaca aaatttctta
     1441 acactttctt cgacaattcc aggtcaattt ctctcttgtt tagcgaagat tgtgccagaa
     1501 gagatatcag ggcgccttcc agttcccgca cgttgctgtt aatattataa gccaggtatt
     1561 taaccacttc cttcggcatc tccagtccgt cattcttcat ctttctttcc aggatctcga
     1621 tccgggtttc ataatccggg atctgaaggt ccgcactaag cccccagcgg aagcggctga
     1681 gcagtctttc ctgcacccct tccagatctt tgggcggctt gtccgaggtc aggatcagct
     1741 gtttaccgga ctggtgcaga tggttgaaaa tggcaaagaa ggcatcctgg gaccgttccg
     1801 cgcggttgaa gaactgcaca tcatcaatga tcagcacatc caccagctgg taaaaatgaa
     1861 tgaaatcatt gacggcatta ttgcggctgt gatcaataaa ctgattgatg aatttctccg
     1921 aactcacata caataccact ttattgtggt gtgtgcgccg gacttcgttg ccaatggcct
     1981 gggccaggtg tgtttttccc aaaccgaccc cgccgtaaat gaccagggga ttaaaggaat
     2041 tggctcccgg tttttccgcc accgtttttc ccgcccggcg ggccacacgg ttgcagtctc
     2101 cttctataaa ggcatcaaag gtataaacgg gattcagctg ggcatcgatc tgcattttct
     2161 tgagacccgg aataacaaaa gggtttttga ccggattatt gataatcagc ggaaaatcca
     2221 tttcattatt tgaaaccgtt ttataactat gtgccggtac gtccatcgtc aaaggtttgt
     2281 tttgcttgtt ccccccatcc accatgatgc gatattccag tctcgcttct tttcccagaa
     2341 tcc
//
LOCUS       DASYCU010000603         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4337098, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000603 DASYCU010000000
VERSION     DASYCU010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4337098"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VGM24_12120"
     CDS             complement(<1..561)
                     /locus_tag="VGM24_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4156173.1"
                     /translation="MIVNQPEQSRKKIALVANSTWSMYNFRIDLIRHLLLSFEVLIIA
                     PGDEFAEDLKKAGCSHIDIRFNNRSENPLQDYALLKSLERIYRTEQPDFIFHYVIKPN
                     IYGSMAAARCGILSVAVITGLGYTFDRPNWLNRIVRFLYRKALKKAREVWFLNQEDAD
                     IFIQRKLVHREKIKILPGEGINTSYFF"
     gene            620..1903
                     /locus_tag="VGM24_12125"
     CDS             620..1903
                     /locus_tag="VGM24_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156174.1"
                     /translation="MLTYFLFFFYLFSGTILLHIIVRRKVSPFTIYHTVAIVLFKVFM
                     GCLYGWVFLHYYNGDDTWNYFNESKDETTLLMQHPLRFFHPFSPLYALQVNGNHGWDA
                     FAFYVNHFERLFVIKGLAILNLLTGKSYYTDVLLFEFLVIAGPFLLFKLLAREFPRRT
                     GMNFLLVFFIPSISFWCSGIRSEALILLFITLAIYNGNEYVQNPNGKSAAGIGLAFAG
                     FLLFRFQYLVFFLPAFLAYLISVRKNRSGPVYFNRVYAAVLLIFIGSLFLPPAYQLSR
                     PMIHSQQSFFELHGNTRYALDSLQPGPLSFFKILPQAIANSSFRPYPWEGKGLLQSLS
                     SIESIFLFAGFLFFILSFPAKNKGKGPLLWFFIYYGVSQIIAIGYSVPFPGAIVRYRS
                     IPLLFIFLFLFSANNILHQKLTRGLFPKTLNKIIM"
     gene            2051..>2943
                     /locus_tag="VGM24_12130"
     CDS             2051..>2943
                     /locus_tag="VGM24_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEY4156175.1"
                     /translation="MESLRVKAINDLSTQSYQPELDGSPRITVYYGENVFNLKTARAF
                     LSDEAYKSLSGSVKGGKRIDRAVANQIAAGLLQWSEGKGVTHYTHWFQPLTGSSAEKH
                     DSFFTIKGDGTPIEQFDGDALIQQEPDASSFPSGGIRATFEARGYTAWDPSSPPFIME
                     IGSGKTLCIPTIFVSYTGETLDYKAPLLKSLEAMNKAAVEVCNYFDKNISRVNATLGW
                     EQEYFVVDEALYNGRPDLVMSGRTVFGHAPAKGQQLEDHYFGAIPERVYVFMRDFEEE
                     AYKLGIPLKTRHNEVAPSQFE"
BASE COUNT          741 a          688 c          643 g          871 t
ORIGIN      
        1 aaaaaaatag ctggtattaa tcccttcgcc gggtaatatc tttatttttt ccctatgcac
       61 cagttttcgc tgaataaaaa tatccgcatc ttcctggttc aggaaccata cttcccgggc
      121 tttcttcaac gcctttcgat aaagaaaccg gacaatcctg tttagccagt tcggacgatc
      181 gaatgtataa ccaagtccgg taatgacggc cacggacaga attccgcacc gggctgctgc
      241 catcgaacca tagatattcg gcttgatcac ataatgaaaa ataaaatcag gctgttcagt
      301 ccggtagatc ctttcaaggg actttaataa agcataatcc tgtaaaggat tctcggaacg
      361 gttattgaat cgaatatcta tgtgggaaca acctgctttc ttcagatctt cggcgaattc
      421 atctccgggt gctatgatga gtacctcaaa actaagcaga agatgacgaa taaggtctat
      481 ccggaaatta tacattgacc atgtactatt ggcaacaagt gcgattttct ttctgctctg
      541 ttcgggctga tttacgatca tccggtttta ttaaattcaa atttatagat ttgggtaatc
      601 aaaatcaggt aaagttccta tgctgaccta ctttctgttt ttcttttacc tgttttccgg
      661 aactatttta ctgcatatta ttgttcggcg gaaagtgtct ccatttacga tttatcatac
      721 agtagccatt gttttattta aggtgttcat gggctgtctg tatggatggg tatttcttca
      781 ttattataac ggcgacgaca cgtggaatta tttcaatgaa tcaaaagatg agactaccct
      841 gctgatgcag catccgctcc ggttcttcca tcccttttca ccactctatg cacttcaggt
      901 aaatgggaat catggctggg acgcttttgc attttatgta aatcattttg aaagattgtt
      961 tgtgatcaaa ggattggcga tactgaatct gttgaccgga aaaagttatt ataccgatgt
     1021 tctgctattt gaatttttag tgatcgccgg tccgtttctg ttgtttaaac tgctggccag
     1081 ggaattcccg cgccgcaccg gcatgaattt tctgctggtc ttttttattc catccatcag
     1141 cttctggtgc agcggtatcc ggtccgaggc cctgatcctt ttattcatca ccctcgccat
     1201 ttataacggc aatgaatatg tgcaaaatcc gaacgggaaa agcgcggcgg gaatcgggct
     1261 ggccttcgcc ggtttcctcc tcttccggtt ccagtacctg gtctttttcc tgccggcttt
     1321 tcttgcctac ctgatcagcg taagaaagaa ccggtccggc ccggtttatt tcaaccgggt
     1381 ttacgcggcc gtcctgctga tatttatcgg aagcctgttt cttcccccgg cttatcagtt
     1441 gtcgcggccg atgattcatt cccagcagag ttttttcgag ttacacggta atacccggta
     1501 tgcgctcgac agccttcagc cggggcctct ctcttttttt aagatacttc cccaggcaat
     1561 cgccaatagc agctttcgac cttatccctg ggaagggaaa ggtttattgc aatccctctc
     1621 ctcgatagaa agcatttttt tatttgccgg ttttctcttt tttattttaa gctttcccgc
     1681 aaagaacaaa gggaaagggc ccttactctg gtttttcatc tattacggag tcagccagat
     1741 cattgccatc gggtatagcg tgccctttcc gggagccatc gtccggtatc gctccattcc
     1801 gcttcttttt atattcctgt tccttttctc cgcaaataat atcctgcatc aaaaactgac
     1861 aagaggcttg ttcccaaaaa cattaaacaa aatcataatg tgaatatcgg ccttaaaaaa
     1921 tattgaataa atggcaaaaa acgggcttat tattgcagga aaccggggta aatcataaaa
     1981 aacgaataga aaatcgattt tttttgcttc ttatgaatat tcgcctacat tcgttcccaa
     2041 atttatttaa atggaatcct taagagtaaa agccattaat gatctgtcga cacagtctta
     2101 tcaacccgaa cttgacggat ctcccagaat cactgtttat tatggagaaa acgtattcaa
     2161 tctcaaaact gcccgtgcat ttctgagcga cgaagcatat aaaagtcttt ccggctcggt
     2221 gaagggagga aaaagaattg accgtgcggt ggcgaaccag attgccgcag gtcttcttca
     2281 atggtctgaa ggcaagggcg tgactcacta tacccattgg ttccagccgc tgacggggtc
     2341 ctctgcagaa aagcatgatt cctttttcac gataaaggga gacggaactc cgatcgagca
     2401 attcgacggg gatgccttga tccagcagga accggacgct tcctcttttc caagcggtgg
     2461 tatccgggcc acgtttgaag cccgcggata taccgcctgg gatccttctt cccccccctt
     2521 tatcatggag atcgggtcgg gaaaaacact ttgcataccg actattttcg tctcgtatac
     2581 cggggagacc ctcgattaca aagctcctct cctgaaatcg ctggaggcca tgaacaaggc
     2641 cgcggtggaa gtctgtaatt attttgacaa gaatatttcc cgcgtaaatg ccaccctggg
     2701 ctgggaacag gaatatttcg tggtggatga ggctttgtat aacggccggc ccgacctggt
     2761 gatgagcggc agaaccgtat ttggacatgc tccggcaaaa ggacagcagc tcgaagacca
     2821 ttattttggt gcgatcccgg aacgcgttta tgtctttatg cgggactttg aagaagaagc
     2881 gtataagctc ggcattccgc ttaaaacgcg gcataacgaa gtggcgcctt cccagtttga
     2941 atg
//
LOCUS       DASYCU010000604         5163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_566731, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000604 DASYCU010000000
VERSION     DASYCU010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566731"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VGM24_12135"
     CDS             complement(<1..343)
                     /locus_tag="VGM24_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4156176.1"
                     /translation="MITTGLTDTEIISRVLHGEQAIFAQLVERYQNYVFTLVLRLIEN
                     REDAEETAQDVFVKAYRSLADFRGESKFSTWLFTIARTSSISFLRKKKLDIRSLDNER
                     TGLQVENKESGF"
     gene            686..1084
                     /locus_tag="VGM24_12140"
     CDS             686..1084
                     /locus_tag="VGM24_12140"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEY4156177.1"
                     /translation="MGPDQLVFFWLITSSLALFAMIFGIRYLNNKENMSMIEKGIDPK
                     IKRDRPAPAPFRNLKWGLVLVGAGIGLFLAYLLDNFLLSAVRHAGDNFSDDGANVPIY
                     FALIAIGGGLGLIISYRIEKKEVLDKPESR"
     gene            complement(1092..2708)
                     /locus_tag="VGM24_12145"
     CDS             complement(1092..2708)
                     /locus_tag="VGM24_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY4156178.1"
                     /translation="MKKLNVWLPLLFAVVMVLGMLIGFRLYPNVHTGGFFKTGKLNRV
                     QEVLDIINSKYVDTVYADSLSEDAIDGMLGHLDPHSYFIPAVDREEMNEDLQGNFEGI
                     GVEFQILSDTVNVTNILSGGPSDRAGLQVGDQFIKVNDSLVAGNGITAEKIKKMLRGP
                     GASIAHLDLLREHTRKLIDVARGTIPLPALDAAYMLNKESGYIRINKFSRTTYQEFQT
                     ALDKLLKQGLKKLVLDIRGNGGGIVDEAVNMADEFLGNDELIVYTEGRKSPRKEYHAQ
                     RPGNFETGKLILLVDEGTASASEILAGALQDWDRASIVGRRTFGKGLVQEPFMLSDGS
                     ELRLTIAHYYTPLGRSIQKPYNNGREAYDDEVIERFHNGEAIHGDTSTTHPGPVYKTP
                     AGRLVYGGGGITPDVFVGFDTNRISKSITSLYVNGTLGNYIYEYYRYHLSEFRQFRDE
                     ADFSARFNKDDELWAGLSNYVKKDSVSLASISPGDKALLQTRIRALLARLIWRTEGYF
                     MVMNQKDPVIKKALELLNDNSPMPSAAKTH"
     gene            2840..3682
                     /locus_tag="VGM24_12150"
     CDS             2840..3682
                     /locus_tag="VGM24_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HEY4156179.1"
                     /translation="MSMLIADSGSTKCEWCLVREGRKRVMETQGASPYFLNEIQLVEL
                     MRNELLPGLKKDHPEEIFFYGTGCAAPANRALVKRALKTVFPGAWIEVEHDLTGAARG
                     LCGKDKGVVAILGTGSNSGYYNGKRIMKNSPGLGFILGDEGSGAYLGRKVLQYYLYGS
                     FEEELSSKFDHKYHTDAAGILDRVYKKPLPNRYIASYALFLAENRGHYMIENIIEDGL
                     NDFFFNHLCRYPEIWKVPVHFVGSVAYGFQDILKELCNTYKWEMGRVLRKPMDGLIAY
                     HKSK"
     gene            3682..4494
                     /locus_tag="VGM24_12155"
     CDS             3682..4494
                     /locus_tag="VGM24_12155"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEY4156180.1"
                     /translation="MEFEKITEEQGSNRHLEKMSIHELLTLMNREDRQVPKAVEKAIP
                     QIEKLAELITDKMLAGGRLFYLGAGTSGRLGILDASEIPPTFGMPYGLIIGLIAGGEE
                     AIRTPVENAEDDRNQGWLDLCEYEVNEKDVVIGIAASGTTPYVIGALETCRGNKISTG
                     CVTCNRGTPLAGVSDYPVEVVVGPEFVTGSTRMKSGTAQKMVLNMISTSVMIRIGRVE
                     DTRMVNMQLTNSKLIDRGTRMLMNQTGIRDYPEAKALLLKYGSVKKAAESRR"
     gene            4564..5148
                     /locus_tag="VGM24_12160"
     CDS             4564..5148
                     /locus_tag="VGM24_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156181.1"
                     /translation="MHDIEPYYNWRHIYVSEDDERSPFFGRIYSEFEYTQAIYNYLIH
                     PQWDDFGSRTLYLKVLLADYEEKYVIIELIGEWNDAIENDIMSLKREVIDPLMLQGIY
                     KFILIAENVLNFHSGDKDYYEEWQEELQEHDGWAVCINMPEQTQYDFSRAKLDRYIDL
                     LDQPDWRIYKPFHLFRLVEAYEKNRLGGSGGTAK"
BASE COUNT         1310 a         1254 c         1360 g         1239 t
ORIGIN      
        1 ggaatccgga ttctttattc tccacctgaa gcccggtccg ctcattgtca agggaccgga
       61 tatcaagctt cttttttctt aaaaagctga tactgctggt ccttgcaatg gtaaataacc
      121 aggtgctgaa cttggattct ccccggaaat cggcgaggga ccggtaggcc tttacaaaaa
      181 catcctgggc ggtctcttct gcgtcttccc ggttttcgat aagccggagc acgagcgtga
      241 atacataatt ttgataacgc tccaccagct gggcgaatat agcctgttcc ccatgcagta
      301 ccctgctgat gatctcggta tcggtgagtc cggttgttat cacaaaaata agacggcaac
      361 cccgcctgtg aggttacaca gtaagttacg ctcaagtgcc taattataaa agaatgaggt
      421 tccgcttacc ccgtttaggt aaattgggtg atcttttgcc ggacagaggt ggaatggaac
      481 gggaaggggg gtgttcactc cttaattctc taaaaaccgg caggatgggg gccagggctc
      541 ctgtaacctg ctgagttcag gcttcgtcct atctccaggc aggagactgc atgcaggaac
      601 aggagaaaac agctgtaacc tttgttccga acaggtagtc tgataacaat gaacctgtat
      661 ttccgtaaaa ataaaattca ttaaaatggg acccgatcaa ttggtatttt tttggctgat
      721 cacgagtagc ctggcacttt tcgctatgat attcggcatc cggtatctga acaataagga
      781 gaatatgtcg atgatagaaa agggcataga ccctaaaatc aagcgggacc gtccggcacc
      841 cgctcccttc cgcaacctga aatggggatt ggtactggta ggagccggta tcgggttgtt
      901 cctggcttac ctgctggata actttttact cagtgcggtc cgccatgcag gcgacaattt
      961 ttccgacgac ggggcgaacg tacccatcta tttcgcactg atcgccatcg gtggcggttt
     1021 gggcctcatc atcagttacc ggattgaaaa aaaggaagtg ctggataagc cggaatccag
     1081 gtaacctgaa attaatgcgt ctttgctgcg gaaggcattg gactgttgtc attcaatagc
     1141 tccaatgcct tttttatcac cgggtctttt tgattcatca ccatgaaata tccttcggtt
     1201 cgccagatca gcctggctaa cagggccctg atccgcgttt gcagcagggc cttatcgccg
     1261 ggagaaatgc tggcaagcga aacagaatcc ttcttcacat agttactcag gcccgcccat
     1321 aattcatcat ctttattgaa ccgggccgaa aagtcggcct catcccggaa ctgcctgaat
     1381 tcagacaggt gatacctgta atattcgtaa atataattgc cgagggtacc attcacatat
     1441 aaggaggtta tgcttttcga aatccggttg gtatcgaagc ccacaaaaac atccggcgta
     1501 attccgcccc cgccgtacac caatcttccc gcaggcgttt tatacacggg tccgggatgg
     1561 gtggttgagg tatctccgtg aatagcctct ccattgtgaa accgttcaat gacttcgtca
     1621 tcgtacgcct cccgtccatt gttatacggc ttttgaatac ttcgtcccag cggagtataa
     1681 taatgggcga tggttaaacg cagttcgctg ccatcgctca gcataaaagg ttcctgcacg
     1741 agcccctttc cgaaagtccg ccggccgaca atgctggccc ggtcccagtc ctgcaacgcc
     1801 ccggccagaa tttcgctcgc agaagcggtg ccttcgtcca cgagcaggat cagttttccg
     1861 gtttcaaaat tccccggccg ctgggcatgg tattccttac gcgggctctt tctgccttcc
     1921 gtataaacga tcagttcgtc atttccgaga aattcatcgg ccatatttac ggcttcgtcc
     1981 acgatgccgc cgccattgcc ccggatgtcg aggaccagtt ttttcaaccc ctgcttgagg
     2041 agtttatcca gtgcagtctg gaattcctga taggtggtgc gggaaaattt attgatccgg
     2101 atatagccgg attccttgtt cagcatataa gccgcgtcca gcgcgggcag cggaatggtt
     2161 ccgcgcgcta cgtcgatcaa tttccgggta tgctcccgga gcaggtccag gtgggcaatg
     2221 ctggcccccg gaccccgcag catcttcttg attttttctg cggtgatccc gttgccggcc
     2281 accagggagt cattcacttt gatgaactgg tcgccaacct gtaatccggc ccggtcgctc
     2341 gggccgccgc tgagaatatt tgttacgttc acggtatcgc tgagaatctg gaattcaact
     2401 ccgatccctt caaaatttcc ctggaggtcc tcattcatct cctcccggtc aacggccgga
     2461 ataaaatagg aatgcggatc caggtggcca agcattccgt cgatcgcatc ttcgctcagg
     2521 gaatccgcat aaaccgtgtc cacatacttg ctgttgatga tatcgagcac ctcctggacc
     2581 cggttgagct tgcccgtttt aaagaatccg ccggtatgga cattgggata aagcctgaac
     2641 ccgatcagca tcccgaggac catgaccacc gcaaacaaga ggggaagcca tacattgagt
     2701 tttttcattt actttatttc tccctggtgg gctgcaaatt aagcaaatga gcggaaccgg
     2761 aagaatgttc agggtgaatg gtttgtcaaa aattgcatta taccttcgcc ccggaattta
     2821 aaggaagaaa aacattaata tgagtatgct gatcgctgac agcgggtcca ccaaatgcga
     2881 atggtgcctg gtccgggaag gtaggaaaag ggttatggag acccaggggg ccagccccta
     2941 ttttttgaat gagatccagc tggtggagct gatgcggaat gaattattgc cgggcctgaa
     3001 aaaggatcat ccggaagaaa tattttttta tggaaccggc tgcgcggccc ccgccaaccg
     3061 ggccctggtg aaaagagctc ttaaaacggt ttttccgggc gcctggattg aagtggaaca
     3121 tgacctgacc ggagcggccc gggggctttg cggaaaggac aagggcgttg tggccatcct
     3181 gggaacggga agcaactcgg gatattacaa tgggaaacgc attatgaaaa acagcccggg
     3241 cctgggtttt atcctggggg atgaggggag cggagcctat ctgggcagaa aagtgcttca
     3301 atattatttg tacggaagct ttgaagagga gctttcctcc aaattcgatc ataaatacca
     3361 tacggacgct gccgggatcc tggaccgggt atataaaaaa ccactgccta accgttatat
     3421 tgcttcttat gccctgttcc tggccgaaaa ccggggacac tatatgatcg aaaatattat
     3481 tgaagatggc ctcaatgatt ttttctttaa tcacctttgc agatatccgg agatctggaa
     3541 agtgccggtg cattttgtgg gcagcgtggc gtatggattt caggatattc ttaaagaact
     3601 ttgcaacacg tataaatggg aaatgggaag ggtcttgcgg aagccgatgg acggactgat
     3661 tgcctatcat aaatcaaaat aatggaattc gaaaaaatca cggaagaaca gggtagcaac
     3721 cggcatctgg aaaagatgag cattcatgaa ctgcttaccc tgatgaaccg ggaagaccgg
     3781 caggtgccga aagcagtaga aaaagcaatc ccgcaaatcg aaaaactcgc tgaactcatt
     3841 accgacaaga tgctggccgg aggaagattg ttttatctgg gagccggaac cagcggccgc
     3901 ctgggcatat tggatgccag tgaaattccg cctactttcg gaatgcccta cgggttaatc
     3961 atcggactga tcgcgggcgg ggaagaagcc atccggaccc ccgtagaaaa tgcggaagac
     4021 gaccggaacc agggctggct ggacttgtgt gaatatgaag tcaatgaaaa agatgtggtc
     4081 atcggcattg ccgcaagcgg tacgacccct tatgtgatcg gcgcgctgga aacctgcagg
     4141 ggaaataaga tcagtaccgg ttgtgttacc tgcaaccgcg gaacgccgct ggccggcgta
     4201 tcggattacc cggtggaagt ggtggtagga cctgaattcg ttaccggaag cacccgtatg
     4261 aaaagtggca ctgcccagaa aatggtgctg aatatgatct ctacttcggt catgatccgg
     4321 atcggccggg tggaagatac ccgtatggtg aatatgcagc tgaccaatag caaactgatt
     4381 gaccgcggta cccggatgct gatgaaccag accggcatca gggactatcc cgaagcaaaa
     4441 gctctgctcc tgaaatacgg aagtgtgaag aaggctgcgg aaagccggcg gtaggccgtg
     4501 ctcatccggg ttcgccagga cagccgtcaa attcctgaat ttattaattt cgcccccggc
     4561 caaatgcacg acatagaacc atattacaac tggcggcata tttatgtgag tgaggatgac
     4621 gaacggtcac ccttcttcgg aaggatctat tccgaatttg agtataccca ggccatttat
     4681 aattacctga tccatccgca atgggatgat ttcggttccc gcactttata cctgaaagtg
     4741 ctcctggcgg attatgaaga gaaatatgtg atcatcgaat tgatcggtga atggaacgac
     4801 gccattgaaa acgacatcat gagcctgaag cgggaggtta ttgacccgct gatgcttcag
     4861 gggatttata aatttatcct catcgccgaa aacgtactca acttccacag cggcgacaag
     4921 gattattatg aagaatggca ggaagaactg caggagcacg acgggtgggc ggtttgtatc
     4981 aatatgccgg aacaaacaca gtacgatttc agccgggcaa agctcgaccg ctatatcgac
     5041 ctgctggacc agccggactg gcgcatctat aaacccttcc atctgtttcg cctggtggag
     5101 gcttatgaga agaacaggct ggggggtagc ggcggaacag ccaaataaaa atttgcggga
     5161 tcg
//
LOCUS       DASYCU010000605         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3900610, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000605 DASYCU010000000
VERSION     DASYCU010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3900610"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1338
                     /locus_tag="VGM24_12165"
     CDS             205..1338
                     /locus_tag="VGM24_12165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4156182.1"
                     /translation="MVQREKIRVLECIRQGKIGGGESYLLGLAENLDKTAFEPIVLAF
                     TDGPMVARLKAQNIQTHIIYTERPFDWRVWKKVMELMIKERIDIVHAHGTRANSNVFW
                     AAKKLKLPLVYTCHAWSFHRDQNYFVKKIRIGSERFLTGKADVNVCGSVANGQEGRKW
                     FKHFSPRIIYNSIDPKKFNPYGRYKNVRAELGVGENELVLTSIARFTLQKQPLKLIQA
                     FKIIAEQVPDIRLLMVGEGEEKEEAVKFINQWGLKDKIILQPFREDIPDILSASDIFI
                     LPSLWEAFPIALLEAMSMGKAVVGTRVDGTPEIIEDRVNGLLIPVENMVKHMVDAIRL
                     LAEDSSLRKNLQLKAIETIYGKYNVDVLAKENEKIYYELMANA"
     gene            1340..2089
                     /locus_tag="VGM24_12170"
     CDS             1340..2089
                     /locus_tag="VGM24_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY4156183.1"
                     /translation="MEYNSVIDIKRLQFITKELQGRLPPGAEVLDVGCGNGIISRSLG
                     KSGFKVKGIDVSDKAIAKARQLNTLPNVQFEVVSAETLVADGHRYHAVICSEVLEHLN
                     KPEKLVQVLYEVLDPGGVLIVTVPNGRGPRETFVTKPFIALRKKDNWLWKLANKIKTF
                     LGYSGSTVQSDADDLTHVQFFTKNSLKELARKNNFEIVRFGKTNFIEDVFPFSFVTKR
                     IKILQKLDCAVAEILPYRFTGGFVTVWEKAK"
     gene            2094..>3066
                     /locus_tag="VGM24_12175"
     CDS             2094..>3066
                     /locus_tag="VGM24_12175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4156184.1"
                     /translation="MEKNKIALLAEKVRTITIPGAKIVNITYVPGKYVKELDGWRGIS
                     IFFVALLHYFPSYFMGGWIGMELIFVVSGFLITGILLDSKSSKNYYKKFITRRVLRIF
                     PLYYLCLVGLFFLLPRSWLDLSYYRSHQAWFWLYAENWLFSFEGWPTVKALRHFWSLA
                     IEEQFYITWPLFVWLFSSKGLIRFCIFLFFFSFFFRNFGMHMGFVMPFPYVATLGRME
                     PIVLGAIIAILVRTNKSILERMVYPVTLITAILATTVFLIAGTMNMEYSLIYMVNYTL
                     LDIFFAGIIVLTICKDEMIYFKRWVLNPPFFKQLGLMSYCIYIFHRPI"
BASE COUNT          789 a          676 c          770 g          831 t
ORIGIN      
        1 ttgaaccgga cccctgtcat ccctgctatt ttcaccctaa gccgttccgg gctttattcg
       61 gtgacctgcc ctgttttcag gcctgtcatt gaccgcgcat ccacgcgctt aagctctttc
      121 ttcaaaggca tagccgggaa tccgggcggt tccaaacatt caatcattaa ttaagcttat
      181 ccatttaatt attttccagc ctgcatggtt caaagagaaa aaatacgcgt attggaatgc
      241 atccggcagg gcaagattgg cgggggagaa tcctatttgc ttggccttgc ggagaacctg
      301 gataaaaccg cttttgaacc catcgtgctg gcgtttaccg acggacccat ggtcgccagg
      361 ctgaaggcac agaatattca gacccatatt atatatacgg aaagaccgtt tgactggcgg
      421 gtatggaaaa aggtgatgga attgatgata aaagaacgga tcgatattgt gcatgcgcat
      481 ggaacccggg ccaattccaa tgttttctgg gcggcaaaaa agttgaaact tcccctggta
      541 tatacctgcc atgcctggtc atttcaccgg gatcaaaatt attttgtcaa aaaaataaga
      601 attggttccg aacgtttctt aacgggcaaa gccgacgtga atgtctgcgg atccgtggcg
      661 aacggacagg aaggtagaaa atggtttaag catttcagcc caaggattat ttataattca
      721 atagacccta aaaagttcaa tccgtacggc aggtataaga atgtccgggc agaactgggt
      781 gtgggagaaa atgagcttgt cctgacgtcc attgcccggt tcacgctgca aaaacagccg
      841 ctaaagctca ttcaggcatt taaaataatt gcggagcagg taccggatat ccggttatta
      901 atggttgggg aaggggagga aaaggaagaa gcggtaaagt tcatcaacca gtgggggtta
      961 aaggataaga tcatcctgca gccctttcgg gaggatatcc cggacatcct ttccgcttcg
     1021 gatatattta ttctgccttc cctctgggaa gcttttccca tcgcgctgct ggaagccatg
     1081 tccatgggta aagcggtggt gggcacccgg gtagacggta cgccggagat cattgaggac
     1141 cgggtgaacg gtttgctcat ccccgtggaa aatatggtga agcatatggt ggatgcgata
     1201 agattgctgg cagaggatag cagtctccgg aagaatcttc agcttaaggc gattgaaacc
     1261 atttacggga agtataatgt ggatgtactg gcgaaagaaa atgaaaagat ttattacgag
     1321 ttaatggcaa atgcttaata tggagtataa cagtgtaatt gatatcaaaa gattacagtt
     1381 cattacgaag gaattacagg gacggctgcc gccgggggcc gaggtgctgg atgtgggttg
     1441 cggcaatggc atcatttcca ggagcctggg aaaatccggt tttaaggtga aagggatcga
     1501 cgtgagcgat aaggcgattg caaaagccag gcagttgaat accctcccga atgtgcagtt
     1561 tgaggtagtg agtgcggaaa cgctggtggc cgacggacac cggtatcatg ccgtaatctg
     1621 cagtgaagtg ctggagcatt tgaacaaacc ggagaaactc gtgcaggtgt tatacgaggt
     1681 gctggatccc ggcggggttt taatcgtaac cgtgcccaac ggccggggtc cccgcgaaac
     1741 ctttgtgacc aaacccttta tcgcccttcg aaaaaaggac aactggctgt ggaaactcgc
     1801 aaataaaata aagacttttc tcgggtacag cggaagcacc gtccaatcgg atgcagatga
     1861 cctgacgcat gttcagtttt tcacgaagaa ttcgttaaaa gaacttgccc ggaaaaataa
     1921 tttcgagatc gtgcgttttg gcaagaccaa tttcattgaa gatgtgtttc cgttttcctt
     1981 tgtaactaaa agaataaaga tcctgcagaa gctggattgt gccgttgccg aaatattgcc
     2041 ttaccggttt accggcggct ttgtaacggt gtgggaaaaa gcaaaatgac cttatggaaa
     2101 agaacaagat cgctttactg gccgagaaag tgcgtacgat aactataccc ggagccaaaa
     2161 ttgtaaacat cacctatgtt cccggaaaat atgtaaagga gctggacggc tggcgcggca
     2221 tatccatatt ctttgtagcc ctgctgcatt atttcccttc ctattttatg ggcggctgga
     2281 tcggcatgga actgattttt gtggtaagcg gtttcctcat caccggcatt ttgctggatt
     2341 ccaaatccag caagaactat tacaagaaat ttatcacccg gcgcgttctg cggatatttc
     2401 ctttatacta tctctgcctt gtgggtctat tctttctgct accccggtcc tggcttgatc
     2461 tttcttatta ccggtcgcat caggcctggt tttggctgta tgccgaaaac tggctttttt
     2521 cttttgaagg ctggcctacg gtaaaagccc ttcgccattt ctggtccctg gccatcgaag
     2581 agcaattcta tattacctgg cccttgtttg tctggctttt ttcttccaag ggattgatca
     2641 ggttttgcat tttccttttc tttttcagtt tctttttcag gaatttcgga atgcacatgg
     2701 gttttgtaat gccttttccc tatgtggcca cgctcggcag gatggaaccc atcgtattgg
     2761 gcgccatcat agccatcctg gtccggacca ataaatccat tctggaaaga atggtgtacc
     2821 cggtgacgct gataacggcc atcctggcga cgaccgtttt tcttatcgcc ggcaccatga
     2881 acatggaata ttcactgatc tacatggtga attatacgct cctggatatt ttctttgccg
     2941 gtattatcgt actcaccatc tgtaaggatg agatgatcta ttttaaacga tgggtactga
     3001 atcctccatt ctttaaacaa ctgggcctga tgtcctattg catctatatc ttccaccgcc
     3061 cgatcc
//
LOCUS       DASYCU010000606         4220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3467149, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000606 DASYCU010000000
VERSION     DASYCU010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3467149"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1234
                     /locus_tag="VGM24_12180"
     CDS             281..1234
                     /locus_tag="VGM24_12180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4156185.1"
                     /translation="MIPCYNCSSYLIETLRAVLQQDPGPEIMQIEVVDDFSTDTNVEA
                     LVDYIGKGRVKYYRQPENVGSLRNFETCLNRSTGEYIHLLHGDDLVKPGFYEEIRQVF
                     SRFPQAGAAFTGYTYINGQNTELYHNNEFLTEPGVLNNWLLEIGRTQHIQPPAMVVKR
                     EVYEKLGGFFAVHYGEDWEMWVRIAAHYPVAHSPKRLAFYRVHEDNITSRYLLSGQSI
                     TDVLTVIDIIQQYFPKSERKRMRRKARKHFSNYFSHTSDRIYHGYFRPDVALMQAKRA
                     LKIHFSYTTLFYFVKTYLKIRAGYKTEKERKFSFRPLNLFR"
     gene            complement(1205..2410)
                     /locus_tag="VGM24_12185"
     CDS             complement(1205..2410)
                     /locus_tag="VGM24_12185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4156186.1"
                     /translation="MTAIQPEIPRTGLKYQPQLSGLRFCAVLFVVVYHFSNALTNLKY
                     VYDLGVFIVFFFVLSSYLITRILLDAKQKAIKNGVGKWKVAVAFLTRRTLRIFPAYYL
                     YLIILILLPLEGREVREHLGIYFFYISNIWLFITKTWQPFVVHLWTLAVEEQFYLLWP
                     WILLFVPDKHLPKVFIAMILAGMIFRVASVTRMTNQNIEIFPMLVLLPACIDCFAAGA
                     LLAYFHNRGFTRNRWLKWLLIAIIPVWFYLIHFHSYHRRIFIGMDRVFVSLFAVTIID
                     TANRGYKGITKKFLENSVVQYLSKISYGIYLYHLIVTVFFWKILDAAQHYFSGCDLSV
                     IGRAAESRYGGFWIYMLLAIACASISWYGLEQPIDRLRKRIFYVRPKDQAGKGKTADS
                     LPEQVKRPE"
     gene            complement(2635..3906)
                     /locus_tag="VGM24_12190"
     CDS             complement(2635..3906)
                     /locus_tag="VGM24_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156187.1"
                     /translation="MKRIFLSLALAGLFSGLFAQSLDKAKEYLKGKKLPEAKTEIDNA
                     LTVEKNQKNAEVWYTKVKIYNAIAADSLLRSTVPDAYVQSLEALKKYAALDDKKLVLL
                     TLDQYKPINEIYQGLFQRGATNYNEKKYAEAYTDFTNAIQAITFMNEKGWIKQNMDTT
                     STLYAGISAEKAGKRDDAAIYYKKIADSGITKIGGNDMAEIYKWIADYYNRKGDKANT
                     EKYVDIGKAKYPSELFFDEIILDDLRKNGPKDSLFARYEMINKEHPDSAIYFFNYGLE
                     LYQYASDTTKGAPPANADELIKRSQEKLSKSLQLNPNYPQASLVMGQISYNQGIELQQ
                     QAKAIKGSKPEDLKKRADLKAEAIKKFDEAIPYFEKIDQLLGKEGVLKRADKNALRDS
                     YDLMVTIYEQKKDKEKAAAWTDKYNNVEKIH"
     gene            complement(3939..>4220)
                     /locus_tag="VGM24_12195"
     CDS             complement(3939..>4220)
                     /locus_tag="VGM24_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY4156188.1"
                     /translation="GGKGIKTINVTEKTGKLVGILDVKEKEDLMITCKSGITIRMPVN
                     GISELGRATQGVKLIRLDDGDGIAAITQLDEESTAEENPDPASSEISPL"
BASE COUNT         1079 a         1030 c          950 g         1161 t
ORIGIN      
        1 gaagtccccc gcggttttct agtggctgat tctgaacaat tagcagctgg aaactgtccg
       61 tttgatccaa atccggaatg aatgcattca tcgaaatgag ccgcggtgga tatgaaccaa
      121 tcgtaattct ttttacctta gtatcggata agtatttact cttatgccta tccctgaacc
      181 ctggttaacg aaagcttatt tcatgacaga tcgtattcca caaactcccc cggtaatcag
      241 gccggtacct gatcaggtaa gaaggccatt gtggtccgtc atgatcccct gctataattg
      301 cagttcctat ctgattgaaa ccctgcgggc ggtattacag caggatcccg gccctgaaat
      361 catgcagatc gaagtggtgg atgacttcag cacggatacc aatgtggaag cgctggtaga
      421 ttatattgga aaaggccgcg tgaaatacta ccgccagccg gaaaatgtcg gaagcctccg
      481 caattttgaa acctgtctca accgttctac cggtgaatat attcatctat tgcatgggga
      541 cgacctggta aaacccggct tctatgagga gatccggcaa gtcttttccc ggtttccgca
      601 agcaggcgca gcctttaccg gctataccta tatcaacgga caaaacacgg agttatatca
      661 taataatgaa ttcctgaccg aaccgggcgt gttgaataac tggcttttgg aaatcggccg
      721 gacccagcat atacagccac cggcgatggt ggtcaaaagg gaggtctacg aaaaactggg
      781 cggctttttt gccgttcact acggagagga ttgggaaatg tgggtcagaa ttgcagctca
      841 ttatcccgtt gcgcactcac ctaaaagact ggctttttac cgtgttcatg aggataatat
      901 cacgagccgt tacctgctat ccggacagag tatcaccgat gtgctgacgg tgatcgatat
      961 cattcagcaa tatttcccca aatccgaaag aaaacggatg cggcgaaaag caagaaaaca
     1021 tttctcaaac tatttctccc acacttcaga ccggatttac cacggatatt tccggcccga
     1081 cgttgccctt atgcaggcaa aacgggcgct gaagattcat ttcagttata ccacgctttt
     1141 ctattttgta aagacctacc tgaaaatccg ggccggatat aaaacggaga aagagagaaa
     1201 attttcattc cggccgctta acctgttcag gtagcgaatc cgctgttttt cccttgcctg
     1261 cctgatcttt aggccgaaca taaaagatcc gcttccgtaa ccggtcgatg ggttgttcca
     1321 ggccatacca ggaaatgctg gcgcaggcaa tggccagtag catatagatc cagaaaccgc
     1381 catatcggga ctccgccgcc cggccaatga cagagagatc gcagccgctg aaatagtgct
     1441 gcgcggcatc taaaattttc cagaagaata ccgtaacgat caggtgatac aaataaatgc
     1501 cataacttat tttggagaga tattgcacta cgctgttttc caggaatttt tttgtgatcc
     1561 ccttatagcc gcggttggcg gtgtctatga tcgtaacggc aaaaagggaa acaaataccc
     1621 ggtccattcc gataaatatc cgccgatggt acgaatgaaa atggatcagg taaaaccata
     1681 ccggaataat ggcaatcagc agccatttca gccagcggtt ccgggtaaat ccccggttat
     1741 gaaaataggc cagcagggca cccgcggcaa aacaatctat gcaggccggt aatagaacca
     1801 gcatcggaaa tatttcaata ttctggtttg tcattcgcgt aacggacgca acacggaaga
     1861 tcatcccggc caggatcatc gcgatgaaaa ctttggggag atgcttgtcc ggcacgaaca
     1921 gaaggatcca gggccataac aaataaaatt gttcttccac cgcaagggtc cacaaatgaa
     1981 cgacaaaggg ctgccaggtc tttgtaatga acagccatat attggatata tagaaaaaat
     2041 agattcccag gtgttcccgg acttcgcgac cttccagcgg caacaaaatc aaaatgatca
     2101 ggtaaagata ataggccgga aaaatgcgca gcgttctccg ggtgagaaac gcaaccgcca
     2161 ctttccattt gccgacacca ttttttatgg ccttttgttt ggcatccagt aaaatgcggg
     2221 tgatgagata gctgcttaaa acaaaaaaga aaacgataaa tacgcccagg tcgtaaacat
     2281 atttgaggtt ggtcagcgca ttggaaaaat gataaacgac cacaaacagc acagcacaga
     2341 accggagtcc ggaaagctgg ggttgatatt tcagacccgt gcgggggatc tcaggttgaa
     2401 tggctgtcaa gggtattctt tagtttaaat agatcatcgg gaaatccagg tcttttatag
     2461 aacggatgat tccccaattt agtacagttt tacattatct gccgcatcgt ccggtgggtc
     2521 cgcccttccg ctttccaggg ccgggattcc aatttttcca atccggttcg aagtaaataa
     2581 aaaatccgcg cagcggttgt cagcaaccat tgcgcggatc accggaaaaa gtcctcagtg
     2641 gattttttcc acgttattat acttgtccgt ccaggcagct gccttttcct tatccttttt
     2701 ctgctcgtaa atcgtcacca tcaggtcata ggaatctctc aacgcgtttt tatctgcccg
     2761 tttcaacacc ccttctttac ccagcagctg atcaatcttt tcaaagtagg gaatggcttc
     2821 atcgaacttc ttgatggcct ctgccttcag gtctgcccgc tttttcaaat cctccggttt
     2881 acttcccttg atcgcttttg cctgctgctg cagttcgatc ccctggttgt aggagatctg
     2941 acccattacg agggaggcct gcggataatt gggattcagt tgcaggcttt tactcagctt
     3001 ttcctgggat cgcttaatca gctcgtctgc gttggccgga ggcgccccct tggtcgtatc
     3061 ggaggcatac tgataaagtt ccaggccata attaaagaaa taaatcgcgc tgtccgggtg
     3121 ttccttattg atcatttcat accgcgcaaa aagcgaatcc ttcgggccat tttttctcag
     3181 gtcgtccagt atgatctcgt caaagaaaag ttcgctggga tattttgcct tcccgatgtc
     3241 tacatatttc tccgtattgg ccttatcccc tttccggttg taatagtctg ctatccattt
     3301 atagatttca gccatgtcat tccccccgat cttggtgatc ccggagtctg cgatcttttt
     3361 atagtaaatg gccgcatcgt cccgttttcc cgctttctcg gccgagatac ccgcatacaa
     3421 ggtggacgtg gtatccatat tctgctttat ccagcctttt tcattcatga acgtaatggc
     3481 ctggatcgca ttggtaaaat cagtatacgc ttccgcgtat ttcttttcat tataattggt
     3541 agctccccgc tgaaacaggc cctggtaaat ttcattaatg ggtttatact gatcgagcgt
     3601 gagcaatacc agtttcttat catccagggc ggcatatttt ttaagagctt ccaaagattg
     3661 gacgtaggcg tccggaacgg ttgaccgcag cagactgtcc gcagcaatgg cattgtatat
     3721 ttttactttg gtataccata cttcggcatt tttctgattt ttttcgaccg tcagtgcatt
     3781 atcaatttcc gttttggctt ccggcagttt tttccctttc aggtattctt ttgctttatc
     3841 caaggactga gcaaataatc ccgaaaaaag gcctgccagt gcaaggctta aaaagatccg
     3901 tttcatgaga ttttttattt gaagttttca gatgacaatt ataacgggct aatttcggat
     3961 gacgccggat ccggattttc ctccgccgtg ctttcctcat ccagttgggt gatcgcagcg
     4021 attccatccc cgtcgtccag ccggataagt tttacgccct gggtagcacg gcccagttca
     4081 ctgattccat tgaccggcat ccggatggta atacctgact tgcaggtgat catcaggtct
     4141 tctttttcct tcacatccag gatcccgaca agtttaccgg tcttttcagt aacgttaatt
     4201 gtttttatgc cctttcctcc
//
LOCUS       DASYCU010000607         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2305503, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000607 DASYCU010000000
VERSION     DASYCU010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305503"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2481
                     /locus_tag="VGM24_12200"
     CDS             <1..2481
                     /locus_tag="VGM24_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4156189.1"
                     /translation="HHRMKSFLLIHLFGLVSGFSFAQTNSFNPPLTGLSGSLSGIVSD
                     SSSGNPVSSATVYIADLKLGAIADAAGRYHFSNLPSGTYLIEVHAIGYSATTRDVQVS
                     GVMEQDFKLLQEYLEESPVVITGLSKATQIRRSPVPVVSVSHRAIVDNLSTNIIDAIA
                     KVPGLSTLTTGPNVSKPFIRGLGYNRILTLYDGVRQEGQQWGDEHGIEVDQYGIDRVE
                     VIKGPASLTYGSDALAGVVNLIPTPPAPDGKVIGDVVAEYQTNNGMFGGSGMVGGNSG
                     GFEWMGRVSHKQAADYQNKIDGRVYGTAFDENDATLSLGLHRAFGYSHLSMSLYDDLQ
                     EIPDGSRDSVTRKFTKQISEDDLYRPIVSDAELKSYAITPLHQHVQHYRIYSSNHFIV
                     GNGSIGVNLGFQRSIRREYDHPEQADIPGLDLRLNSYVYDLKYFFPSFNGWELTTGIN
                     GMYQQNRVTNGTEFIIPSYQQFDLGPFAMIKKTFNKLDLSGGIRYDSRSFSNQELYTA
                     ENPVTHFDGPVYGADTVGAAKPFSGYHHVFSGASGSIGASYSFSNKLSAKLNISRGYR
                     APNISEISSNGVHPGTGIFQIGNPSFKPEFSLQEDAGITYTNRYMVINASVFYNAISN
                     YIFNEKLVGADGKDSILSGNSAYAFKQGKAALYGGEFSVDIHPVKPLHFENSISLVYG
                     NLLGASGGFVKNDSNRYLPFITPVHGLSELKYDFQNKRLHLVNGFVKIEADWYAAQDR
                     VFLQGNTETPTPGYTLINVGAGTGITNRKNRTVMNVYVMANNLFDVAYQDHLSRLKYF
                     EPYPTDPRPYHGIYNMGRNLSLKLDFPF"
     gene            complement(2506..>3286)
                     /gene="ruvB"
                     /locus_tag="VGM24_12205"
     CDS             complement(2506..>3286)
                     /gene="ruvB"
                     /locus_tag="VGM24_12205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY4156190.1"
                     /translation="SIKETSGPVIEKPSDLAGLLTNLGPHDVLFIDEIHRLSTVVEEY
                     LYAAMEDYRIDIMIDTGPNARSIQLSLNPFTLIGATTRSGLLTAPLLSRFGIKSRLEY
                     YDSMTLQKIIQRSASILHTRITSDASAEISRRSRGTPRIANGLLRRVRDFAQVLGNGI
                     IDMDITKHALRALNVDEHGLDEMDNRILNTIIGKFKGGPVGLNTISTAVGEEAGTIED
                     VYEPFLIQEGFLMRTPRGREATAKAYAHLGKKPGSDEILLF"
BASE COUNT          756 a          797 c          911 g          822 t
ORIGIN      
        1 catcatcgta tgaaatcatt cttacttatc catctgtttg gcctggtttc cggattttct
       61 tttgctcaga ccaattcttt caacccgccc ctgacgggcc ttagtggttc cctttcggga
      121 atagtgagcg acagcagcag cggcaatccc gtatcctccg ccaccgttta tattgcggat
      181 ctcaaactgg gggccattgc cgatgctgcg ggccgttacc atttctccaa tcttccttcg
      241 ggaacctatc tcatcgaagt gcatgcgatc ggttattcgg caaccacccg ggatgtgcag
      301 gtatccggcg tgatggaaca ggattttaag ttactccagg aatatctgga agaaagtccg
      361 gtggtgatca ccggcctgtc caaagccact cagatcagga gaagtccggt tccggtggta
      421 tcggtgagtc acagagccat cgtggataac ctgagcacca atattattga tgccattgcg
      481 aaggttcccg gcctgtcaac gcttaccacc ggaccgaatg tttccaaacc ctttatccgg
      541 ggactgggtt ataatcgcat actcacgctg tatgacgggg tgcggcagga gggtcagcaa
      601 tggggggatg agcatggcat tgaggtagac cagtacggaa ttgaccgggt ggaagtgatc
      661 aagggccccg ccagtctcac gtatggatcg gacgcgttgg ccggcgtggt gaacctgatc
      721 ccgacgccgc ctgcgccgga cgggaaagtg atcggggatg tggtggcgga atatcaaacg
      781 aacaatggca tgtttggcgg atcgggtatg gtaggcggca attccggggg gtttgaatgg
      841 atgggaaggg tttctcataa gcaggctgcg gattatcaga ataaaataga cggaagggtg
      901 tacggaaccg catttgacga gaacgacgcc accctgtcct taggtcttca ccgggccttc
      961 ggctattcgc atctgagtat gtcactctat gacgatctgc aggagatccc ggatggaagc
     1021 cgggattccg ttacccgcaa attcaccaaa cagatttcag aagacgattt gtaccgtccc
     1081 atcgtatcgg atgccgaatt gaaatcttat gccattacgc cattgcatca gcatgtgcag
     1141 cattaccgga tctattcttc gaatcatttt attgtcggga atggcagtat cggcgtgaac
     1201 ctgggctttc agcgaagtat ccgccgcgaa tacgatcatc cggagcaggc ggatattccc
     1261 ggattggatc ttcggctcaa ttcctatgtg tacgacctga aatatttttt tccatccttt
     1321 aacggatggg aactgaccac cgggatcaat ggcatgtatc agcaaaaccg ggtaacgaac
     1381 gggacggagt ttatcattcc atcttatcag cagttcgacc tggggccctt tgccatgatc
     1441 aaaaaaacat ttaataaact ggatctttcg ggaggcatcc ggtacgatag ccgatccttc
     1501 agcaatcagg aactgtatac ggcggaaaac ccggtgaccc atttcgacgg gccggtttat
     1561 ggggcggata ccgtgggtgc ggccaaaccg ttctccggtt atcatcatgt attttccgga
     1621 gcatccggaa gtatcggcgc cagctactct ttttcgaata aattatcggc caagctgaat
     1681 atttccaggg gataccgggc gccgaatatt tcggagatct cctccaacgg tgttcatccg
     1741 ggaaccggca tttttcagat cggcaaccct tcgttcaaac cggaattcag tttacaggaa
     1801 gatgccggta taacgtatac caatcggtat atggttatca acgcgagtgt tttttacaat
     1861 gcgatttcca actatatatt caatgaaaaa ttagtgggcg ccgatggaaa ggattcgatc
     1921 ctgtccggaa acagtgcgta tgcctttaag cagggaaagg cggcattgta cggtggcgag
     1981 ttcagtgtgg acatccatcc ggttaaaccg ctgcattttg agaacagcat ttccctggtt
     2041 tacggaaatc tgctgggtgc ttccggtggt tttgtgaaga acgacagcaa ccggtatctg
     2101 cctttcataa cgcccgtgca tgggctgtct gaattgaaat atgattttca aaacaagcgc
     2161 cttcacctgg tgaatggatt cgtgaaaata gaagcggatt ggtacgcggc ccaggaccgg
     2221 gttttcctgc aaggcaatac cgaaacgccc acaccaggat acacgcttat caatgtcggt
     2281 gccggcacgg gaataacgaa ccggaagaac cgcacggtga tgaatgttta tgtgatggct
     2341 aacaacctgt ttgacgtggc ataccaggat cacctgagcc ggctgaaata tttcgagcct
     2401 tatccgactg acccgcggcc ttatcatgga atatataata tggggaggaa tctgagcctg
     2461 aagctggatt ttccgttttg acatccttca ttctaaaaac gggtcctaaa ataacaggat
     2521 ctcatcgctg ccgggttttt ttccgagatg cgcataggct ttggcagttg cttcgcggcc
     2581 ccggggggtg cgcataagaa atccttcctg tatgaggaag ggttcataaa catcctcgat
     2641 ggtgccggct tcttccccca ccgcggtgga aatggtattg agcccgacgg gccctccctt
     2701 gaattttccg ataatggtgt tgaggatgcg gttgtccatt tcatccagcc cgtgctcatc
     2761 cacattgagt gcgcgcaaag cgtgtttggt gatatccatg tcgatgatgc cgttaccgag
     2821 cacctgggcg aaatcgcgca cgcggcgcag tagcccgttg gctatcctgg gtgttccgcg
     2881 gctgcggcgg gaaatttctg ctgaagcgtc tgatgtgatc cgggtatgaa gtatggaggc
     2941 cgaccgctgg atgatctttt gcagggtcat gctgtcgtaa tattccagcc tggatttgat
     3001 cccgaaccgc gacagaagcg gggcggtaag caggccgctc cgggtagtgg ccccgatcag
     3061 ggtgaacgga ttcagcgaaa gctggatgct cctggcattg ggaccggtgt cgatcatgat
     3121 gtcgatccgg tagtcttcca tcgccgcata gagatattct tccacgaccg tgctgaggcg
     3181 gtgaatttca tcaatgaaga gtacgtcgtg gggcccgaga ttggtgagga gccccgcgag
     3241 gtcgctgggt ttttcgatta cgggccctga agtttcttta atgctt
//
LOCUS       DASYCU010000608         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3900916, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000608 DASYCU010000000
VERSION     DASYCU010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3900916"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VGM24_12210"
     CDS             complement(<1..1124)
                     /locus_tag="VGM24_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HEY4156191.1"
                     /translation="MNTLLSIARLFPFCAICLSLAMGSCRKSPSASTSDPGAGDTSTI
                     TPPVEPPLANTVGFFLDDWTPATFSPPFFEDVQVSGTATNTVTVDPSAIITKIPQPVF
                     GQNAVFWLGPADAEPLFMNPLKNLQPHILRFPGGSASDAYFWNAPQGINPPDAPALIM
                     DKDGHQTAPGYNYGMTDLNWQCSLNNYYHILQQSNSEGLLTVNYGYARYGTGANPVAA
                     AAHLAADWVRYDNGRTRYWEIGNENFGDWEWGYRIDLGANKDGQPEYITGALYATHFQ
                     VFADSMRQAASETGKKIYIGAVTSEAPATESWQTNTLKTWNSGMMSGINEKADFYVVH
                     NYFTPFNTNSNAADVLASAAGVPAQMISYVTQTLQSNGATV"
     gene            complement(1121..1357)
                     /locus_tag="VGM24_12215"
     CDS             complement(1121..1357)
                     /locus_tag="VGM24_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156192.1"
                     /translation="MRIVKYLLLFIPVALASCVAGSGHYVYSQPVYVYPGVGLGTIIA
                     VVVSWSRNKSILWAIIHGILGWLYVIYALLIPKR"
     gene            complement(1483..>2990)
                     /locus_tag="VGM24_12220"
     CDS             complement(1483..>2990)
                     /locus_tag="VGM24_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEY4156193.1"
                     /translation="LKIIPPGKVIVPDEKIFELPEKVLQFGTGMLLRGLPDYFIDKAN
                     RMDIFNGRVIIVKTTSRGDADVFDKQDGLYTLCVRGVEDGKKIGENSINSSISRVLNA
                     QHEWKQILACAHNPDLQVIISNTTEIGIKLVNDDIRHQPPVSYPGKLLAFLYERFKAF
                     NGRAQSGFVIVPTELIPDNGNKLQSIVLELAHINKLEEAFIGWVERCNYFCNSLVDRI
                     VTGTPDAEIKAATEKELGYTDQLITFCEVYRLWAIEGDERIRKILSFCQADEGVRIEP
                     DIDLYRELKLRLLNGTHTLSCGVAFLAGLDTVKEAMDDEMTGKYIAGLMQEEIGPSIP
                     YEIEPAVWRLYITKVCDRFRNPHIKHYWINITLNYSYKIRMRCLPLLKNYYKNNGSVP
                     ERIALGFAAYLRFMKAVKQADNKYYGEFNGKSYIIDDETAGEFYAIWQEQSPDKLVAD
                     VLKNTALWGEDLSVLPGFQESVTGKLNSILRDGMRITMESAYQKNVLSGTS"
BASE COUNT          722 a          743 c          748 g          777 t
ORIGIN      
        1 acggtcgctc cgttggactg aagggtttgc gttacgtagc tgatcatttg tgccggcacg
       61 ccggcggcgc ttgccagcac gtctgccgca tttgaattgg tattaaaagg agtgaaataa
      121 ttatggacga cataaaaatc cgctttttca tttatgccgg acatcattcc ggaattccag
      181 gttttcagtg tattggtctg ccaggattct gttgccggag cttctgatgt cacggcgccg
      241 atataaatct ttttaccggt ttcggatgcg gcctgccgca tggaatcggc aaacacctgg
      301 aaatgggtcg catataatgc gccggtaata tattcgggct gcccatcttt attcgcgccc
      361 aggtcgatcc ggtagcccca ttcccagtct ccgaaatttt catttccgat ttcccaatac
      421 cgggtgcggc cattgtcgta ccggacccag tctgccgcca gatgagccgc agccgctacg
      481 ggattcgcac cggtcccata acgggcatag ccatagttca ccgtaaggag gccctcgctg
      541 ttgctctgct gaagaatgtg gtaataattg ttcagggaac attgccagtt cagatcggtc
      601 attccgtaat tataaccggg tgcggtctgg tgtccatctt tatccatgat cagcgcgggt
      661 gcatccggag gattgattcc ctgcggggca ttccagaaat acgcatcgct tgcgctgcct
      721 ccgggaaatc gcagaatatg aggctgtaga tttttgagcg ggttcatgaa taagggctct
      781 gcatctgccg gacccagcca gaaaacagcg ttttgtccga agaccggctg cgggattttg
      841 gtaatgatgg cggaggggtc caccgttacg gtattcgttg ccgttccgga aacctggaca
      901 tcttcgaaaa agggcgggct gaaggtggcg ggggtccagt cgtctaaaaa gaatcccacc
      961 gtattcgcaa gcggcggttc caccggagga gtaatggtgc tggtatctcc ggcacccgga
     1021 tctgaagtgc tcgcggaggg gcttttcctg caggagccca tggccaggga caggcagatg
     1081 gcgcagaagg ggaaaagacg ggcaatggac agaagcgtgt tcatctttta ggaatcagta
     1141 aggcataaat aacatagagc cacccgagaa taccatgaat gatcgcccac aaaatggatt
     1201 tattcctgct ccaggaaacc accacggcga taatggttcc aagccctact ccgggataaa
     1261 catatacggg ttgtgaatag acgtaatgac cggaaccggc aacgcaggaa gccagcgcaa
     1321 ccggaatgaa caaaagaaga tattttacaa tacgcatggg cgattatttt tatcggagga
     1381 ataggcgcat ataaaaataa ggcagattat ggcgataagg gaatatattg aaaaataaac
     1441 cggatcctgt tccggtcgca aaagccgata accgccggca tttcatgaag tgccggagag
     1501 aacattcttt tgataagcgc tttccatagt aatccgcatt ccatcccgga ggatgctgtt
     1561 taatttgccg gtaactgatt cttgaaatcc gggtagaacg ctcaggtctt ccccccagag
     1621 tgcagtattt tttaagacat ccgcgaccag tttgtccgga gactgctctt gccagatcgc
     1681 gtaaaattct ccggctgttt catcatctat tatataggat ttaccgttga actctccgta
     1741 atatttattg tcggcttgct ttaccgcttt cataaaacgg aggtaggcag cgaatccaag
     1801 ggcaatccgt tcggggacag aaccgttatt tttgtaataa ttcttcagca ggggcaggca
     1861 tcgcatcctt attttatagc tgtagttgag cgtaatattt atccagtagt gtttgatatg
     1921 cggattccgg aaacggtcgc acactttcgt gatataaagc cgccatacag ccggctcgat
     1981 ctcatagggt atggacggtc cgatttcctc ctgcatcaga ccggcaatat attttccggt
     2041 catttcatcg tccatagcct cttttacggt atccaggccg gcaagaaagg caaccccgca
     2101 actcagggta tgcgttccgt tcaataaacg caatttcaat tcacggtata aatcgatatc
     2161 cggctcaatg cggacccctt catccgcttg gcaaaaggac aatatcttcc ggatccgttc
     2221 atcgccttcg attgcccaaa gccggtagac ctcgcaaaag gtgatcagct ggtccgtata
     2281 tcccagttcc ttttcggtag cggcttttat ttcagcatcg ggggtgccgg tgacaatccg
     2341 gtccaccagc gaattgcaga agtaattaca gcgttccacc catccgatga atgcttcttc
     2401 cagtttgttt atatgcgcca gctcgagaac gatggattgc agtttatttc cgttgtccgg
     2461 gatcaattcg gtcgggacaa tcacaaaccc gctttgcgcc ctcccgttaa acgctttaaa
     2521 ccgttcatac agaaaagcaa gtaatttccc ggggtatgaa acagggggtt gatgacggat
     2581 atcgtcattt accagcttga tccctatttc tgtcgtattg gaaatgatca cctgtaaatc
     2641 cgggttgtgc gcgcaggcta agatctgctt ccattcgtgc tgggcattca gcacgcggct
     2701 tatcgatgaa ttgatactgt tttccccgat ctttttgccg tcctccactc cgcgcacgca
     2761 caaggtatag aggccgtcct gtttgtcaaa gacatccgca tcgccccgcg aagtcgtttt
     2821 tacgataatc acccttccgt tgaagatgtc catccggttt gccttgtcaa taaaataatc
     2881 gggcaggccc cggagcaaca tcccggtccc gaactgtaaa accttttccg gtaactcaaa
     2941 tattttctca tctggcacga tgactttgcc gggcggtata atttttaatg
//
LOCUS       DASYCU010000609         5651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5058002, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000609 DASYCU010000000
VERSION     DASYCU010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5058002"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..904
                     /locus_tag="VGM24_12225"
     CDS             2..904
                     /locus_tag="VGM24_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY4156194.1"
                     /translation="MSYGAALAKQTGVQANGVLLGTVTEDITALGKYGIQQVHQVNDE
                     SLNALDPQVYIRLIAEVAQATQATVVVFSHNSSGKAIAPGLSARLKAGLVTGAIALPD
                     TGNGFLVKKNVFSGKASATVSIKTAIKIISLNPNAYPIVSAEGSAKLVTESIRPEPSR
                     VKLKSVSKASGEIPLTEAEIVVSGGRGMKGPENWGPIEELAKLLHAATACSRPVADAG
                     WRPHGEHVGQTGISIAPNLYIAIGISGAIQHLAGVNRSKVIVVINKDPEAPFFKAADY
                     GIAGDAFEVLPKLIQAVRKLRGLA"
     gene            1051..1620
                     /locus_tag="VGM24_12230"
     CDS             1051..1620
                     /locus_tag="VGM24_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEY4156195.1"
                     /translation="MSHSITQTHSYAVVLGEINGLRRLPIVIGGFEAQAIAVALEKMH
                     PSRPLTHDLMKNFMSAFTIDLQEIIISDLQEGIFYSKLICTSEHDTVEIDSRTSDALA
                     LAVRFGCPIFTYENIIESAGILMEDSAGKKKTPKETVTTEGGAKEDLGGLSLEDLNAL
                     LSEVLEQEDYIRAIKIRDEINSRKKGRHG"
     gene            1617..2426
                     /gene="ispE"
                     /locus_tag="VGM24_12235"
     CDS             1617..2426
                     /gene="ispE"
                     /locus_tag="VGM24_12235"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY4156196.1"
                     /translation="MIGFPPAKINLGLSVLSRRSDGFHNLETVFYPVRLRDILEIIPA
                     SGNTLRISGLPVPGDPKDNLLWRAYKLLKSGFPQIPPVEIQLFKSIPSGAGLGGGSSD
                     AACMLNLLNTCFDLNIPGEKLASYALSLGSDCPFFLNPSPSFARGRGEILEPVSLDLS
                     GYSILLVHPGKPVSTAWAYSRIRPAGATFSIKELMAEPPANCRDRLQNDFEIPVFQQY
                     PELQKIKTALYEAGALYASLSGSGSSLFGIFKKTQVPPNIISAELQQSFLS"
     gene            2664..3893
                     /gene="rocD"
                     /locus_tag="VGM24_12240"
     CDS             2664..3893
                     /gene="rocD"
                     /locus_tag="VGM24_12240"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEY4156197.1"
                     /translation="MTSLYSEKTGRFLDLENEYGAHNYHPLPVVLSKGEGVFVWDVDG
                     KKYYDFLSGYSALNQGHCHPKIVASLIQQVQKLTLTSRAFCNDQFGGFARFATSLFGY
                     AKLLPMNTGVEAVETAIKLCRRWGYDVKGIPENKARIIVCSGNFHGRTSSVISFSTDP
                     AAFTGFGPYMPGFDIIPFNDLEALADALQNREVAGFLVEPIQGEAGVVVPDEGYLKTA
                     ARYCKDANVLFIADEIQTGLARTGKMLACDHEGVRPDLLILGKALSGGMLPVSAVLAD
                     DDVMLTIKPGEHGSTYGGNPLASAIAITSLQVILEEGLAANAAVQGEYLRKGIESFHS
                     PFIEAVRGKGLLNAMVIRHPDPEAAWHVCLNLMENGLLAKPTHGDKIRFAPPLTITRT
                     QVDECLDIIGDSLRILK"
     gene            3936..4679
                     /locus_tag="VGM24_12245"
     CDS             3936..4679
                     /locus_tag="VGM24_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY4156198.1"
                     /translation="MNSPEKADLPVKLPDHAHNEEHVFGADVLRDVVIGMSDGLTVPF
                     ALAAGLSGAVSSTSLIIIAGLAEIAAGSIAMGLGGYLAGKTEAEHYHSELQREYDEVE
                     KVPEMEKQELRTFFSSLGLSREVQEQAVEEVSQDKEKWVELMMKYELGLEKPDPRRAK
                     KSAFNIGFSYIVGGLIPLSPYFFVSNGIEGLKVSAVITLICLFVFGYIKSKFTGVNRW
                     EGAMRVMIIGAIAAGCAFAIARLIQTGHY"
     gene            complement(4673..5074)
                     /locus_tag="VGM24_12250"
     CDS             complement(4673..5074)
                     /locus_tag="VGM24_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156199.1"
                     /translation="MKYAPYIGILAVIVLILSCFMSWAYYPDINQTFTGFYSNQNIYG
                     KPGRAFVFLAVCICILFLIPKLWAKRISQFLGILILAYAIKSFILFTSCYNGICPVKK
                     TGVFLMLISSLFILLAGLFTGVRLPDESKNQ"
     gene            complement(5090..>5651)
                     /gene="dapF"
                     /locus_tag="VGM24_12255"
     CDS             complement(5090..>5651)
                     /gene="dapF"
                     /locus_tag="VGM24_12255"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY4156200.1"
                     /translation="GRCMVRFAYHLGMHKTMYHFLASDGDHEAEIDDGTSIINLKMRD
                     VSGIREENGDYILNTGSPHYVKIVDDVMDFDVLKNGIDIRYNEEYTREGINVNFVEQK
                     KTDEIIVRTYERGVEDETFSCGTGVTASAIVCYHNEVGYNDVTVITKGGKLVVKYDRS
                     NNNDYTNIWLCGPAEKVFEGKAESRD"
BASE COUNT         1380 a         1392 c         1458 g         1421 t
ORIGIN      
        1 gctgagttat ggagcggcgc tggctaaaca aacgggcgtg caggcgaacg gagttttgct
       61 gggtaccgtc acggaagata tcaccgccct gggtaaatat ggtattcagc aggtccatca
      121 ggtgaatgac gaatccctga atgcgctcga tcctcaggtt tatatccgcc ttatcgctga
      181 agttgcgcag gcaacgcagg caacggtagt tgtgttttcg cataacagtt cggggaaagc
      241 cattgccccg ggattgtctg cccgtttaaa agcagggctt gttaccggag caattgccct
      301 gccggatacc ggcaatggat ttctcgtgaa gaaaaacgtg ttttcgggca aggcctctgc
      361 tacggtttcg ataaaaacag ccataaaaat catttcactg aatcccaatg cctatcctat
      421 tgtttccgcc gaaggttccg caaaactggt aacggaatcc attcgtccgg aaccatccag
      481 ggtgaaatta aaatccgttt ccaaggcttc cggggagata ccgcttaccg aagctgaaat
      541 agtggtcagt ggcggacggg gcatgaaagg tccggaaaac tggggtccta ttgaagaact
      601 cgcaaagctt ttacatgcgg ccactgcctg cagccggccg gtcgccgatg ccggctggcg
      661 tccgcacgga gagcatgttg gacaaaccgg aatttccatt gcccccaacc tctatattgc
      721 catcggcatt tcgggagcga ttcaacatct ggccggtgta aaccggagta aagtgattgt
      781 agtgatcaac aaagatccgg aagcgccatt tttcaaggcg gctgactatg gaattgccgg
      841 ggatgctttt gaggtgctgc ccaaattaat tcaggcggtc aggaagctcc ggggcctggc
      901 ctgaatagag aattaatcct ttgattctct ctaaattata tttggccttc tgaaattttt
      961 ggaaggcttt agttttttat atttgccctt ttaatattag cctccgggcg ggtccgtttt
     1021 atgaagaaga tcgaattaga aattgtcgct ttgtctcata gcattacaca aacgcattcc
     1081 tatgccgtgg ttttgggcga gatcaatggt ctccggcgcc tgccgatcgt gatcgggggc
     1141 tttgaggccc aggccattgc cgtggccctg gagaaaatgc atcccagccg gccgcttacg
     1201 cacgatctca tgaaaaactt catgagcgcg tttacgattg acctgcagga gattattatc
     1261 tcggacctgc aggaaggaat tttttattcc aagctcatct gcaccagtga acacgatacc
     1321 gttgagatag attcccgcac gtccgatgcc ctggccctgg cagtccggtt cggctgtcct
     1381 attttcacct atgaaaatat cattgaaagc gcgggtatcc tgatggaaga cagcgccggc
     1441 aagaaaaaga cgcccaagga aacggtcacc accgaaggag gtgcgaaaga agatctgggc
     1501 ggtctatccc tggaagacct gaacgccctg ctttccgaag ttctggaaca ggaagattat
     1561 atccgggcca ttaagatacg ggacgagatc aacagccgga aaaaaggccg tcacggatga
     1621 tcggctttcc cccggcgaaa attaacctcg gtctttccgt cctctcccga agatccgacg
     1681 ggtttcacaa tctggaaacg gttttttatc cggtgcgact ccgggatata cttgaaataa
     1741 ttccggcctc cgggaatacc ctccgtatat ccggacttcc cgtgcccgga gatccaaagg
     1801 ataatctgct ctggcgggcc tataaactgc tgaagtccgg gtttccgcag atccctccgg
     1861 tagaaattca actttttaaa tccattccat ccggtgcagg tcttggcggc ggatcttcag
     1921 atgccgcctg tatgctgaac ctgttaaata cctgtttcga cctgaatatc cccggggaaa
     1981 aattagcttc ttatgctttg agcctgggca gcgattgtcc tttttttctg aatccctctc
     2041 ccagttttgc ccggggaagg ggagaaatac tggagccggt ttccctggac ctttccggat
     2101 attccatttt gctcgttcat cccggaaagc ccgtctccac cgcctgggcc tattcccgga
     2161 tccggccggc cggggcaaca ttctccatta aggaactgat ggccgaacca ccggccaatt
     2221 gtcgggaccg gctgcaaaat gattttgaaa ttcctgtttt tcaacaatac ccggaattgc
     2281 aaaaaataaa aacagcatta tatgaggccg gcgcccttta tgcctcgctt agcggcagcg
     2341 gctccagtct gtttggaatt tttaaaaaaa cccaggtccc tccgaatata atttctgcgg
     2401 aactgcagca aagttttctt tcctgatgcg cttaggaatg aaccggaatg caactccacg
     2461 gaatgaccgg ttttgaaaaa ccggatgatt tcgtatcttg cgatacaagc tattcatatc
     2521 tataccagat cttccatacc atttctctcg ggtaacctcg attttctcga tcatcgatga
     2581 gatgaactga tcgtttgccc ggctgcccgg gccttccgtt taagccttct ttttcaataa
     2641 caacaagcgc tatacttatg caaatgacat cactgtattc tgagaagacg ggccgttttc
     2701 tcgacctgga aaatgaatac ggcgcccata actatcatcc gctcccggtg gtcctcagca
     2761 aaggggaagg agtttttgta tgggacgtgg atggaaaaaa atactatgat tttctgagcg
     2821 gttattctgc tctcaaccag ggacattgcc atcccaaaat cgttgccagc ctgattcagc
     2881 aggtgcaaaa gctcacgctt acctcaaggg ctttttgcaa tgatcagttc gggggatttg
     2941 cccggtttgc gacttccctt ttcggttatg cgaaactgtt acccatgaat accggcgtgg
     3001 aggccgtgga gacggcgatt aaactttgcc gccgctgggg atacgacgtc aaaggcattc
     3061 cggaaaacaa agcgcggatc attgtatgtt ccggaaattt tcatggccgc accagctcgg
     3121 tcatttcttt cagcaccgat ccggctgcct ttaccggatt tggcccgtac atgcccggat
     3181 tcgatatcat tccgtttaat gacctggagg cgctggcgga tgcactgcaa aacagggagg
     3241 tggccggatt cctggtggaa cccatccagg gggaagcagg cgtagtggtt ccggacgaag
     3301 gctatttaaa aacagctgcc cggtattgta aggatgccaa cgttttattt attgccgatg
     3361 aaatacagac cggactggca aggaccggca aaatgctggc ctgcgatcat gaaggggttc
     3421 gaccggatct gctgattttg ggaaaagccc tgagcggagg catgctgccg gtctccgccg
     3481 tactggcgga tgatgacgtg atgttaacca taaaacccgg ggagcacgga tccacttatg
     3541 gtggaaatcc gctggcctcc gccattgcca tcacttcgct ccaggtgatc ctggaggaag
     3601 ggctcgccgc caacgcagcc gttcagggag aatatttgcg aaaggggatc gaatccttcc
     3661 attccccttt cattgaagcc gttcgcggaa agggattgct gaatgcgatg gtgatccgtc
     3721 atccggatcc cgaggcagca tggcatgtat gtctgaacct gatggagaac ggattactgg
     3781 ccaaacccac acatggtgat aagatacgct ttgctccgcc gctgaccatc acgcggacgc
     3841 aggtggatga atgcctggat atcattggag acagcctccg tattttaaaa tagtcagccg
     3901 cattaatgct taacttgagc gtctaaattt cttgaatgaa cagcccggaa aaggccgatt
     3961 tgccggttaa gcttcccgat catgcccata atgaagagca tgttttcggg gctgatgtcc
     4021 tgagggatgt ggtcatcggg atgtctgacg gactcaccgt tccctttgcg ctggcagccg
     4081 ggttgagcgg ggcggtgtcc agcaccagcc tgatcatcat cgccggtctt gccgagattg
     4141 ctgcgggttc tattgccatg gggcttgggg gatacctggc aggtaaaacg gaagcggaac
     4201 attaccattc cgagctgcaa cgggaatacg atgaagtgga aaaagttccg gaaatggaaa
     4261 agcaggaact acgcactttt ttctcttcgc ttggcctgag ccgggaagtg caggaacagg
     4321 ctgtggaaga agtgagtcag gataaggaaa aatgggtgga actgatgatg aaatatgaac
     4381 tggggctgga gaagcccgat ccgcggcgcg ccaagaaaag cgctttcaac attggatttt
     4441 cctatatcgt cggcgggctg attccgctca gtccctattt ttttgtatcg aatggtattg
     4501 agggtctgaa agtctcggcg gtaattacgc tgatctgcct gtttgtattc ggatatatca
     4561 agagcaaatt taccggcgtg aaccgctggg aaggcgccat gcgggtaatg attatcggtg
     4621 ctatcgctgc gggttgcgct tttgccatcg ccaggctgat tcaaaccggg cactattgat
     4681 ttttcgattc atccggaagc cgcaccccgg tgaacaaacc ggccagcaat ataaaaagag
     4741 aggaaatcag catcaggaag acaccggttt tttttaccgg gcagattccg ttatagcagg
     4801 aagtgaaaag aataaatgat ttaatggcat aagccaggat aagaatgcct aaaaactggc
     4861 tgatccgttt agcccataat ttcgggatga ggaataatat gcagatacag accgccagaa
     4921 aaacaaatgc cctcccgggc ttcccgtaaa tattctgatt agaataaaag ccggtgaatg
     4981 tctgattaat atccggataa taagcccagg acataaaaca ggagagaatg aggacgataa
     5041 cagccaggat gccaatatag ggagcgtatt tcatcggatg gaatccattt tagtcccggg
     5101 attcggcttt tccttcaaat actttttccg ccggcccgca taaccagata ttcgtatagt
     5161 cgttattgtt actccggtca tatttaacca ccagcttccc tcctttagta attaccgtta
     5221 cgtcgttgta cccgacctca ttgtgataac acacgatagc gctggcggtg actccagtcc
     5281 cgcaggaaaa ggtttcatcc tccacgcccc gttcataggt gcggacaatg atctcatccg
     5341 ttttcttctg ttcgacaaag ttgacattga tcccttctct ggtatattcc tcgttatacc
     5401 ggatatcgat cccgtttttc aggacgtcga agtccatgac atcgtccacg atttttacat
     5461 agtgcggtga tccggtgttc agaatataat cgccgttctc ttcccggatc ccggaaacat
     5521 ctctcatctt caggttgatg atgctggttc cgtcgtcgat ctccgcttca tggtccccgt
     5581 ctgaagcgag aaaatgatac atggttttgt gcatgccgag atggtaggca aaccggacca
     5641 tgcagcgacc g
//
LOCUS       DASYCU010000610         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3467568, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000610 DASYCU010000000
VERSION     DASYCU010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3467568"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VGM24_12260"
     CDS             complement(<1..1109)
                     /locus_tag="VGM24_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156201.1"
                     /translation="MPLRTRAKRLPFLIALLAICSLGLAQSKKRVHHNRKSDSALLEM
                     NSRLSDSLLQEAEIRDTVVPNMVNKVESYSFRLNRAESFLNRKLDTTGILKSLSRLER
                     GLNFLHNRLERNDNSLNLRNLNTASVLLGESQEKLNDWQNLLDGFSDQMDKNHDHIRK
                     IKHDSTLLNTSLDSVLHGEMKTVYQRALSLDSVQHLASAKINALRNRISINNLLLRDL
                     KTEIQYRTQQLRSGMWKPEEPPILNAGVSDYDSSLPSVISEAMSRSLQVLRIFMASTW
                     DTRLINIGIWIVLLLWFLINLYHLKKKKDNESVLKNVKFISRSALFSSLLLLFTYGPF
                     LYESLPAIYLHGNGICRLLVLSVLLLPYLSKTGKW"
     gene            complement(1182..2054)
                     /locus_tag="VGM24_12265"
     CDS             complement(1182..2054)
                     /locus_tag="VGM24_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY4156202.1"
                     /translation="MQESKEIKALFHLIDDPDEEVFTTISDRIISYGRGIIPNLEYLW
                     ENTVQTTIQERIEMLIHRLHYRDLTEDFQRWKGSSYQDLLTATLLVSKFQYPDLSTSA
                     VLQEMEKLRRNIWLELNSYLTPLEQVNVLTSIIYGYYNLKGTEVGYQHPEEFLINKLI
                     ETKRGNTIINGIVYLLLCELLDIPVKVINIPQQFVLAYIKPDFSEEAAMKDPRDRIEF
                     FIDPMSGQVFTHKDVNNYFTRISVTAGPAHFRPANHIRIIQSLLEEFSKCFDNESNLY
                     KRTELLMLSRLLNE"
     gene            complement(2225..4570)
                     /gene="topA"
                     /locus_tag="VGM24_12270"
     CDS             complement(2225..4570)
                     /gene="topA"
                     /locus_tag="VGM24_12270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY4156203.1"
                     /translation="MAKNLLIVESPAKAKTIELILGEDFEVKSCYGHIRDLEKLDMGI
                     DIKNKYKPRYIVPEEKQKVVKELKGLAKKSGEIWLATDEDREGEAISWHLCEVLGLDP
                     KSTKRIVFHEITKPAILSAVQRPRTVDMNLVNAQQARRILDRIVGFELSPVLWRKMSM
                     KNNLSAGRVQSVAVRIIAEREREINAFSPVSTFRIEASFTSRDISGKDVVFPAEGKKF
                     GQEKGAEEFLKSCINAEYRVSDIQVKPGKKSPAAPFTTSTLQQEASRKLGYSVSRTML
                     LAQKLYESGKITYMRTDSVSLSETAMENLKSEINNKFGKKYYQQRTFKNKNESAQEAH
                     EAIRPTYMENANVEDADCRRLYELIWKRTMASQMADAELEKTTAIIQISTNREELKAS
                     GEVLKFDGFLKVYREDRDDEDIPEEDQQEGMLPPLSVNQKLPLLEISATERFSRPQPR
                     YTEASLVKKLEELGIGRPSTYAPTISTILKRGYVEKRDKEGAIRLFRILKLKDNILTK
                     SSGQETTGAEKSKLFPTDLGLVVTDFLKLYFDDIMDYNFTARIEAEFDEVASGKLEWN
                     NMIDEFYLPFKKDVDSTIEKAERISGERELGTDPVTGKKVIARMGRYGPMVQIGEQND
                     EEKPKFAKLKNTQSIETINLDEAMELFRLPRNLGQFEDNEVSVNIGRFGPYIAHDKKF
                     YSLGKEFDPYTISFEESTPIIVEKRAAKEQRTIKVFEKEKIQLLRGPYGPYIKQGLRN
                     YKIPKEKQENVEGLELNEIKAIIEEVKANPPKRVPRKKKSS"
     gene            complement(4682..>5571)
                     /locus_tag="VGM24_12275"
     CDS             complement(4682..>5571)
                     /locus_tag="VGM24_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HEY4156204.1"
                     /translation="DNDELQVNYTRRVDRPNFFQLFPYTDYSDSLNLNKGNPNLKPQF
                     TNSLELNYEKNYGNNSFLASVYYKYTTDLITRYQNKEINPITDSLVLINTFINASSSY
                     VGGLELTARNNVTPWWELTSNLNLFTSKIFTDDSIETAPANYSWFAKINNSFKLPKNF
                     TLQLSGNYTSKTVLPPGGSSGQGGRGWGPTVSGNAQGYSKPTGSVDAALKFEFLKNKA
                     ASLTLSIDDIFKTRQYDVFINSAYSNQEAYRIRDPQFVRLQFHYRFGKFDTSLFKRKN
                     NRNGDQDNMPQNGVEGPMN"
BASE COUNT         1254 a         1352 c         1353 g         1612 t
ORIGIN      
        1 atccatttcc cggttttgct gagatagggt aaaagcagaa cactcagaac gagaagccgg
       61 caaattccgt tgccgtgcag gtatatggcc ggcaggcttt catagagaaa aggaccataa
      121 gtgaataaaa gcagcaggct ggaaaacagg gcggagcggc ttatgaattt tacgttcttt
      181 aaaacggatt cattatcctt tttctttttc aggtgataga gattgatcag gaaccacagc
      241 agcaggacga tccagatgcc gatattgatc aaccgcgtat cccaggtgct ggccataaag
      301 atcctcaata cctgaagaga tcttgacatg gcctcgctga tcaccgaagg cagggaggaa
      361 tcgtaatctg ataccccggc atttaaaatg ggcggctctt ccggtttcca cattccggaa
      421 cgcagttgtt gcgtccggta ctggatctct gttttcaggt ctctcagcaa taaattattt
      481 atggaaatgc ggttccgtag ggcattgatc tttgccgaag cgaggtgctg aacagagtcc
      541 agggacaaag cccgttgata cacagtcttc atttctccgt gcagcacgga atccagcgag
      601 gtgttcagca gggtgctgtc gtgcttgatc ttacggatat gatcatggtt tttatccatt
      661 tgatcgctaa atccgtccaa caggttttgc cagtcgttca gtttttcctg ggattctccg
      721 agcagtacgg aagcggtatt cagattgcga aggttcaggg aattgtcatt tcgctccagc
      781 cggttatgca aaaaattcaa tccgcgctcc agtcttgata aggattttaa tattccggtg
      841 gtatccagtt tccggtttaa aaaggattcc gcccggttca gccggaagga gtaggactcc
      901 accttgttca ccatattggg aaccaccgta tccctgatct ctgcttcctg aagcagggaa
      961 tcggagagcc gcgaattcat ttccagcagc gcgctatcgc ttttccggtt gtggtgaacg
     1021 cgtttcttgg attgtgccag ccccaggctg caaatggcca acagggcaat taaaaaaggg
     1081 agacgtttcg cccgagtcct tagcggcatg atgctggtgt tttatgccgc aaattaagct
     1141 aaatagggag tttgtcggga tccggtctgc catttccggc cttattcatt cagcagccgg
     1201 gaaagcatca ggagctcggt ccttttatac agattggatt cgttgtcgaa gcatttgctg
     1261 aattcctcca acaggctttg gatgatgcgg atatgatttg ccggcctgaa atgagcagga
     1321 cctgcagtaa cggagatccg ggtaaaatag ttgttgacgt ctttgtgcgt aaatacctgg
     1381 ccgctcatgg gatcaataaa aaattcgatc cggtcgcgtg ggtccttcat ggctgcctct
     1441 tcgctaaaat cgggtttgat gtacgccagg acaaactgct gcgggatatt gatgaccttc
     1501 accgggatat ccagcaattc gcatagcagg aggtaaacaa taccgttgat gatcgtattt
     1561 ccccgcttgg tttcaatcag cttattgatc aggaattctt ccggatgctg gtaccccact
     1621 tccgttccct tcaggttata atatccataa ataatactgg tcagcacatt gacctgctcg
     1681 aggggagtca ggtaactgtt cagctcaagc cagatattgc ggcgtaattt ttccatttcc
     1741 tgcagtacgg cactggtact gagatctggg tattgaaatt tggaaaccag cagggtggcc
     1801 gtgagcaaat cctggtaact gctgcctttc cagcgttgga aatcttcggt caggtcccgg
     1861 tagtgcaggc ggtgaatcag catttcaatc cgctcctgga tagtggtctg aacggtattc
     1921 tcccaaagat attccaggtt cgggatgatg cctctcccat aactgataat gcggtcactg
     1981 atcgtggtga acacttcttc gtcgggatca tcgatgagat gaaacagggc tttgatttct
     2041 ttggattcct gcatgggttg gatgttttat cttcaggcga tatacagccg gggataacgg
     2101 agttgaatat cgcggaagtt aaaaaaacgg caatgacaat cttatccaca aacccttccg
     2161 ggttgtttat ttcatggaga aaatgcagtc cgctacattg gataacgctg gaatccggaa
     2221 aatcttatga ggatttcttt ttccggggaa ctcttttggg cgggttggcc ttcacttcct
     2281 caatgatggc tttgatctcg ttcaactcaa gcccttccac gttctcctgt ttttccttcg
     2341 ggatcttgta attgcgcaat ccctgtttta tatagggccc gtaggggccg cgaagtaact
     2401 ggatcttttc tttttcaaat accttaatgg tcctttgttc tttggctgct ctcttttcaa
     2461 cgatgatggg cgttgattcc tcaaagctga tggtgtacgg gtcaaattcc tttccgaggg
     2521 aatagaattt cttgtcatgc gcgatgtagg ggccgaatct tccgatattc acacttacct
     2581 cgttgtcttc aaactgtccg agattccgcg gcagccggaa taattccatc gcttcatcca
     2641 ggttgatggt ttcgatgctc tgggtgttct tgagcttcgc gaatttcggc ttctcctcat
     2701 cgttttgctc tccgatctgc accatcggtc cgtaacgccc catgcgggca atcaccttct
     2761 tacccgtgac cggatcggtg cccagttctc tctccccgct gatccgctct gccttttcga
     2821 tggtactgtc gacgtccttt ttaaaaggca gatagaattc atcgatcata ttgttccatt
     2881 ccagcttacc actggccact tcatcaaatt cggcttcaat ccgcgccgtg aaattgtaat
     2941 ccatgatatc gtcaaaatac aatttcagaa aatcggttac caccagcccc agatcggtgg
     3001 gaaacaattt ggatttttcc gctccggtgg tttcctgccc gctggatttg gtcaggatat
     3061 tgtcctttaa tttcaggatc cggaacaggc ggatcgcccc ttctttatcg cgcttctcca
     3121 catatcctct ttttaatatg gtggatatgg tgggagcata cgtggaaggc ctgccaattc
     3181 ctagctcctc cagtttcttt accagggatg cttcggtata cctgggctgc ggacggctga
     3241 atctttcggt ggcgctgatc tccagcaggg ggagcttttg attgacgctg agcggaggca
     3301 gcatcccttc ctgctgatcc tcttccggta tatcctcatc atcccggtct tcccggtaga
     3361 ctttcaaaaa tccatcaaat ttcaatactt ctccggatgc tttcagttct tccctgttgg
     3421 tggagatctg tatgatcgcc gtggtctttt ccagttccgc atcagccatt tgcgaagcca
     3481 tggttctttt ccagatcaat tcgtaaagac ggcggcagtc cgcatcttcc acgttggcat
     3541 tttccatata ggtcgggcga atggcttcat gcgcttcctg tgcgctttca tttttatttt
     3601 tgaaagtccg ctgctgataa tattttttgc cgaatttgtt attgatctca ctcttcaggt
     3661 tctccatcgc cgtttcactc aggctgacgc tgtcggtccg catgtacgtg atcttgccgc
     3721 tttcatataa tttctgggcg agcagcatcg tgcggctaac cgaataaccc agcttgcggg
     3781 aggcttcctg ttgcagggtg gacgtagtga acggagcggc cggggatttc tttccgggtt
     3841 taacctgaat atccgaaacg cggtattctg catttataca gcttttcagg aactcctctg
     3901 cccccttttc ctgtccgaat tttttacctt ccgccgggaa gacgacatcc tttccggaaa
     3961 tatccctgga agtaaaagaa gcttcgatcc ggaaggtgct cacgggagaa aaagcattga
     4021 tctctctttc ccgttctgca ataatgcgaa ccgccacact ctgaaccctt ccggcactca
     4081 ggttgttttt catgctcatt ttccgccaga gtacggggct gagctcaaat cccacgatgc
     4141 ggtccaggat gcggcgcgcc tgctgggcgt tcaccaggtt catatccacc gttctgggtc
     4201 tctgaacggc ggatagaata gcgggtttgg ttatttcgtg gaaaacaatc cttttggtgc
     4261 ttttgggatc cagccccagc acttcgcaaa gatgccagct gatggcttct ccttcccggt
     4321 cctcatccgt tgccagccag atctctccgg attttttggc caggcccttg agctctttga
     4381 ccactttctg cttttcttcg ggaacaatat atctgggctt atatttattc ttgatatcaa
     4441 tgcccatatc cagtttttcc aggtcccgga tatggccgta gcaacttttc acttcaaaat
     4501 cttctcctaa aatcagttca atcgtctttg cctttgccgg ggactccacg atcaataagt
     4561 tcttcgccat attctaatat aatggggttt acacgcccga aggcgtgcaa ttttacagta
     4621 taacggtgaa agtaaaaaat tgatgagccc cgggagaacg aataaaacag ttcagaactg
     4681 cttagttcat aggtccttcc actccatttt gcggcatatt atcctggtcg ccgttgcggt
     4741 tattcttccg tttaaaaagt gaagtatcga atttaccgaa ccggtaatgg aactgaagcc
     4801 gcacaaactg cggatcccgg atccggtatg cttcctgatt ggaataagcg gagttgataa
     4861 acacgtcgta ttgccgcgtc ttaaagatat cgtcaatgct cagagtgagg ctggccgctt
     4921 tatttttcag gaattcaaat ttcagcgcgg catccacgga acctgttggc ttggaataac
     4981 cctgggcatt tccgctgacg gtagggcccc agccccgtcc gccctgacca gacgatcccc
     5041 cgggaggaag aacggtcttg gaagtatagt taccggaaag ctgaagggta aaattcttcg
     5101 gcagtttaaa gctgttgttg atcttggcaa accagctgta attcgcggga gccgtttcaa
     5161 tggaatcgtc cgtaaagatc ttggacgtaa acagattgag attgctggtg agctcccacc
     5221 agggtgtaac attattcctt gccgtcagtt ccaggccgcc gacataactc gagctggcat
     5281 taataaaggt attgatcagc accagcgaat ccgtaatggg attgatttcc ttattctggt
     5341 aacgggtgat cagatcggtg gtatacttat aatataccga tgccagaaag ctattgttgc
     5401 cgtaattctt ctcatagttc agctccaggg aattggtgaa ctggggcttg aggttcggat
     5461 ttcccttgtt caggttcagc gagtcagaat agtcggtata gggaaagagc tggaaaaaat
     5521 tcggacggtc cacccggcgt gtgtaattca cctgcagctc atcgttgtca t
//
LOCUS       DASYCU010000611         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4189699, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000611 DASYCU010000000
VERSION     DASYCU010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189699"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VGM24_12280"
     CDS             complement(<1..241)
                     /locus_tag="VGM24_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156205.1"
                     /translation="MASLKNMLPDKRRTTVLVSLVFLLAIFLVPYFFVYIPANADSLK
                     RQAFLKLNRAAQNMIAKTNDIGNYYQNNSAGAVTLD"
     gene            783..1271
                     /locus_tag="VGM24_12285"
     CDS             783..1271
                     /locus_tag="VGM24_12285"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEY4156206.1"
                     /translation="MPILILVTGGTFDKEYNELTGELFFKETHLPEMLRLGRNLVETK
                     AVILEMIDSLYMTDEYRERLAVRCREAAEENIIITHGTDTMAETAVYLDKAVRDKTIV
                     LTGAMIPYKFGSSDGLFNLGSAMAFVQSLPQGVYVAMNGSCFPAGKVRKNKVTGLFEK
                     LS"
     gene            1320..2006
                     /locus_tag="VGM24_12290"
     CDS             1320..2006
                     /locus_tag="VGM24_12290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4156207.1"
                     /translation="MDHILDNPIYNALGSGNENFSSGNAGARCFPDDISPLAGLKENS
                     PRDFDALYELSAAGRFFILFTPGEIEVPGHWKVLERMKLLQMVCERLVAGAGRKGEFT
                     TLEERHVQDMLALTKLTNPGPFLPGTIKLGNYIGILSEGRLIAMAGQRLQPGPYIEIS
                     AVCTHPDHLGHGYASLLIREQIRRILAASAIPVLHSREDNSSAVRLYQKLGFTTRRRM
                     MAYALQKQGS"
     gene            2122..2586
                     /locus_tag="VGM24_12295"
     CDS             2122..2586
                     /locus_tag="VGM24_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156208.1"
                     /translation="MKTILTCLECGKRLTEGVEEFSHNVYGYPLCLRHQAYITDSGAS
                     PQAVDVYFALKAKKIPVILEYWDGYKQVDIALPGRLHIEVNTPSSYETVQAFVDLKRS
                     VYSLKENISTIIIPDYLLDDPAMFQLTVEELAKACRSVMNAVPFPNLTISMN"
     gene            complement(2596..3288)
                     /locus_tag="VGM24_12300"
     CDS             complement(2596..3288)
                     /locus_tag="VGM24_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEY4156209.1"
                     /translation="MASIIIKKESLDFLRMLSKNNNREWFNKRKDKYLEARNNIIGFA
                     DALLTEMNKHDRIETQSGKDALFRIYKDVRFSKDKTPYNTHWSGAFKRATKKLRGGYY
                     FNIKPGSSLLAGGFWGPEPQDMKRIREDIGANYEDWRKLLAARPLSNTFGKMTGDQLG
                     TAPRGYYKDHPAIDLLRYKQFILRYEFSDKEVCSPGFLNKMNEVYKKMRPFLNYLSEV
                     LTTDANGVSFIE"
     gene            3497..>4077
                     /locus_tag="VGM24_12305"
     CDS             3497..>4077
                     /locus_tag="VGM24_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HEY4156210.1"
                     /translation="MKTTFLLFIFLAGMISVQAQKPNWQNLDLQKDSVFGISTEKAYS
                     DLLKHKQKKKKYKPVVVAVIDGGVDTGHEDLRSVIWTNPKEIAGNGRDDDNDGYIDDL
                     HGWDFIGGPKGDIDQETLELTRLIRDGKGTPAMLAEYHKQVIKDSAIVKNIQGFQRVL
                     DGMVSKMGKDTPTLADFKSYNAVEPGEQQIKKI"
BASE COUNT         1055 a          967 c          991 g         1064 t
ORIGIN      
        1 gatccagggt aaccgccccg gcgctattgt tctggtaata attgccgatg tcattggttt
       61 tggcaatcat attttgcgcc gcccggttga gcttcagaaa ggcctgtcgc ttcaggctgt
      121 ccgcgttggc cgggatataa acaaaaaaat aagggaccag gaaaattgcc agcaggaaaa
      181 caagggatac caggacggtg gtcctgcgtt tatccggcag catatttttt agactggcca
      241 taattctgaa ggtgagcaac gacgtacaat tctccgggaa tacggatgct aagcaattta
      301 aatagggctg atcatttgtc gcgaagaatg ggtaaaaggg aaacactcca taaaaaatac
      361 ggggtaataa ataagtgttt tcttttatca ccctggactg cagtaaggga agcggatagg
      421 cagccctgtt aaatttaact attttaagga gaaaatctgc ctgaaacgga gttcattttt
      481 aataatcgtc tgcctgaagg cgtttacaaa tagcagttga acttcatcat tcacccgttc
      541 atcccaatga cggtgaatta acattgccct taacagcatg atcggtaatt ttgtatctga
      601 gagagagaaa aaatacaatt tattaaggat ttacaactgt atcctatttc aactttttct
      661 cataagcagt tagttttggt taacggcccc tgtttccaca ggggcttctt tttttgagca
      721 ggaccccgcc cattaagagc tattctcccc ctgtcttata tttgttcccg aaaacataag
      781 gaatgcccat actcatcctg gtcaccggcg gaacgttcga caaagagtat aacgaactga
      841 cgggagagct gttttttaaa gagacccacc tgcccgagat gctgcgcctg ggccgcaacc
      901 tcgttgaaac caaagccgtg atcctggaga tgatcgacag cctgtacatg acggatgaat
      961 accgggagcg cctggccgtc cggtgccggg aggcggccga agaaaatatt attataaccc
     1021 atggcaccga caccatggcc gaaacagcgg tttacctgga caaagcggtt cgggacaaaa
     1081 cgatcgttct caccggcgcc atgatcccct ataaattcgg cagttcggac ggcctgttta
     1141 atttgggaag cgcgatggct tttgtccagt ccctgccgca aggcgtgtac gtggcgatga
     1201 acggcagctg ttttccggcc ggaaaggtgc ggaagaataa ggtaacggga ctgtttgaga
     1261 agctcagctg aaacggttgt attttcatat tctgacggat tccgaaacgg cacaatcaaa
     1321 tggatcatat tctcgacaac cccatatata atgcgcttgg ttcagggaat gaaaatttct
     1381 cttccggaaa tgccggcgcc cgatgcttcc cggacgatat atctccgctg gcggggctaa
     1441 aagaaaattc gccccgggac tttgatgcgc tatatgagct cagtgcggcc ggccgctttt
     1501 tcattttatt tacaccgggg gaaattgaag tccccggcca ttggaaagtc ctggagcgga
     1561 tgaaattact tcagatggtc tgtgagcggc tggtagcggg cgccggaagg aagggagaat
     1621 tcactaccct cgaagaacgg cacgtgcagg acatgcttgc cttaacgaag ctgacaaacc
     1681 ccggtccctt tcttcccgga acgataaagc tgggaaacta tatcgggatt ctttctgaag
     1741 gccggttgat cgctatggcc ggccagcgcc tgcaacccgg accctatatt gagatcagtg
     1801 cggtctgcac ccatccggac catctcgggc acggttatgc gagcctcctg atcagggaac
     1861 agatcaggcg gatcctcgcc gcatcggcca ttccggtgct tcactcccgg gaagacaaca
     1921 gcagcgccgt caggttatat caaaaactgg gttttaccac ccggagacga atgatggcct
     1981 atgcccttca gaaacaggga tcctagttcc ttatccttcg ccttttcccc gtctgccaaa
     2041 acccactttt catcccatat aaaccgccat tcggctaatt gattatgaga tcagcggacc
     2101 atttttgatc tttattctat tatgaaaacg atcctgacct gcctggaatg tggaaagcgt
     2161 cttacagaag gcgtggagga gttttcccat aatgtatacg gttatccgtt atgcctgagg
     2221 caccaggcat atatcaccga ttccggggcg agcccgcagg cagtggatgt ttattttgct
     2281 ttaaaggcga agaagattcc ggttatcctg gaatattggg atggatacaa gcaggtggat
     2341 attgcgcttc cgggcagact gcatatcgag gtcaataccc cctcttcgta cgaaactgtc
     2401 caggcatttg tcgatctgaa acgctcggtt tattcattaa aggaaaatat ttcaaccatt
     2461 atcattccgg attatttact ggacgatccg gccatgttcc agctgacggt tgaagaactc
     2521 gcaaaagcct gccggtccgt aatgaacgcg gtcccttttc caaacctgac catctcgatg
     2581 aattaggcga aactttcact caataaaaga tacgccgttc gcgtccgtcg taagcacttc
     2641 actcaggtaa ttaaggaatg gccgcatttt tttataaacc tcattcattt tatttaaaaa
     2701 accgggactg caaacctcct tgtccgaaaa ttcatagcgg aggataaatt gtttgtaccg
     2761 gagcagatcg atggcgggat gatctttata atatccccgg ggagctgtac ccagctggtc
     2821 cccggtcatt ttaccaaacg tatttgaaag gggcctggca gccagcagtt ttctccagtc
     2881 ttcataattc gccccgatat cctccctgat tcttttcatg tcctggggtt ccggccccca
     2941 aaagccgccg gccaaaaggg aggagccggg cttgatattg aaatagtagc ccccgcgcaa
     3001 ttttttggtt gcccgtttaa aagctccgct ccagtgggta ttgtacggcg tcttgtcttt
     3061 ggaaaagcga acgtccttgt aaatccggaa taaagcgtcc ttgccggact gggtttcgat
     3121 ccggtcatgc ttattcattt ccgtcagcag ggcatccgca aacccgatga tattattgcg
     3181 cgcttcgaga tatttgtcct tccgcttatt gaaccattcc cggttattgt tttttgaaag
     3241 cattctcaaa aaatccaggg attctttttt tattattatg gatgccatag cttgtttgtg
     3301 gtctgttatt tttttatcgt ttccttacga agggccgaat ttagcgtata attctaaatc
     3361 cctttatttc agcctcttaa gtttcaaccg gccctgcctc tttttaaccg ctcctccttt
     3421 tttatgaaga ttgtttttta tgcgcattgt attttcagcc ttaagctgcg tactttcaga
     3481 cttaaattgt aaaaatttga aaacgacttt ccttttattc atattcctgg cgggtatgat
     3541 ttccgtacag gcccagaaac ccaactggca gaacctcgac ctgcaaaaag acagtgtttt
     3601 cggcatcagt acggaaaaag cgtattccga cctgctcaaa cataaacaaa agaagaagaa
     3661 atacaagccg gtcgtagtgg ccgtcatcga cggcggggtg gataccggtc atgaagattt
     3721 aagatcggtg atctggacca atccgaagga aattgccggc aacggccggg atgatgacaa
     3781 cgacggatat atcgacgacc tgcatggatg ggattttatc ggtgggccga aaggagatat
     3841 cgatcaggag acgctggaac ttacgcggct gatccgagac gggaagggca cgcccgccat
     3901 gctggccgaa tatcataaac aggtcataaa agacagcgcc atcgttaaaa atatacaggg
     3961 ttttcagcgg gtgctggacg gcatggtatc gaagatgggc aaggatacgc cgaccctggc
     4021 ggattttaaa agttataacg cggttgagcc cggagaacag cagatcaaaa agattat
//
LOCUS       DASYCU010000612         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_421076, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000612 DASYCU010000000
VERSION     DASYCU010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421076"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="VGM24_12310"
     CDS             <1..1978
                     /locus_tag="VGM24_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY4156211.1"
                     /translation="GASFVASRIFPNREFLTSTDAWSRPVNLYTGPDGALYVLDYYRR
                     VIESPEWMSAEAIAAGGLYDGSDKGRIYRITAAGAGKADWTKGLQLGSATNEELVKNL
                     ANPNEWWRINAQRLLVDRADKSALPALISMAMQQAAPLGRLHALWTLEGMNALTADLI
                     KAALKDSVAGIRENAIRLAERHLSAAPDLAGALLPLQTDPDAKVRFQLLLTLGYLNTP
                     EAVQARNRLLFKDINDKWVQVAALSAPASQTASLLGMVLEKFRENDPAYASLVQRLTT
                     MAGASAGPEDIHRLIQKATDIHTKKQAWQAPILEGLTRGMKNRKPSAAISDADQHLLV
                     KTFFENPSGDLRRASLQLLKLNGISNSSLKTGSDLKAVSMVKDTGIAEERRADAIDWL
                     ALYNPSAHVLLLEKLLVPQEGSLVQLAALRVLNQVPSDSVSRFLIQQWPVLTKEIRDE
                     AIGVLVSKPERIALLIDALEKGKILTSSVSFDRSVQLMMMKDDNLRNRARALFTKNET
                     EAKEINKSYQAALELNGDPLKGKQIYLENCARCHTVRGKLGISFGPDLGTIHNWKKED
                     ILANILNPSLSIAAGYELWEAELKNNETVQGIIASETTSAITLKNSEGNVRTISRQDI
                     RSLKSLNISAMTPGLEKKIDKQQMADILAFLRQN"
     gene            complement(1999..>2623)
                     /locus_tag="VGM24_12315"
     CDS             complement(1999..>2623)
                     /locus_tag="VGM24_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEY4156212.1"
                     /translation="AAFKSAGGDWVSQMATFITTNSLITLPINLLSFTGQCNNDHIVL
                     DWTTASEMNNDYFAIERNDDGTTWKPAGTVKSAGNSSTAQKYSLTTDKANSASSYFRL
                     KQTDVDGNTKYFNTIQVNNCHTDQVGISIYPNPTNGVSLFGRIDLKANEVYSIVIYDT
                     FGRMISSSTSNQPEFTVNFPHTLASGVYYAKFSSEGFSKAACFLVKH"
BASE COUNT          689 a          635 c          722 g          577 t
ORIGIN      
        1 cggggccagt tttgtcgcca gccgtatttt tccgaacagg gaatttttaa cgtcaaccga
       61 tgcgtggtcg cgcccggtga atttatacac cggaccggac ggagcgctgt atgtcctgga
      121 ttattaccgg cgcgtgatcg aatcaccgga atggatgtct gccgaagcga ttgcagccgg
      181 cggcctgtac gatggttccg acaaaggccg tatctaccgc atcaccgcgg ccggcgcggg
      241 aaaggccgac tggacaaaag gactccagct gggaagtgca acaaacgaag agctcgtaaa
      301 aaaccttgcg aatccgaacg agtggtggag aataaatgca cagcgtttgc tggttgaccg
      361 cgcggacaag tcggccctgc cggcgctgat cagcatggcc atgcagcagg ctgctccatt
      421 gggccgcctg catgccctct ggaccctgga aggaatgaat gcattaacgg ctgatcttat
      481 taaagccgca ttgaaggata gcgtggccgg gatccgggag aatgccatcc gactggcgga
      541 acggcattta tcggctgcgc cggatctggc cggggcgctg ctgccgctac aaaccgaccc
      601 ggatgcaaaa gtgaggtttc agctcctgct caccctcgga tatcttaaca ctccagaagc
      661 ggtgcaggcg aggaacaggc ttttgtttaa agatataaat gataaatggg tgcaggttgc
      721 cgcactgtcc gcccccgctt cgcaaacggc ttctttgctg ggaatggtgc tggaaaaatt
      781 ccgggagaat gatccggcct atgcctccct ggttcaacgg ttgacgacaa tggccggagc
      841 aagcgcggga ccggaagata ttcaccggct cattcaaaaa gccacggata ttcacacgaa
      901 aaaacaagcc tggcaggcgc ccatactgga agggcttacc cggggaatga aaaacagaaa
      961 accttccgca gctatttcgg atgcagacca gcatttgctc gttaaaacat tctttgaaaa
     1021 tccttccgga gacctgcggc gggcttccct gcaattgtta aagctgaacg ggatctccaa
     1081 ttcatcgctg aagacgggtt cggaccttaa agcagtaagc atggtaaagg ataccggcat
     1141 cgcggaagag agaagagccg atgcgataga ctggctggcc ctgtacaacc cttccgcgca
     1201 cgtactcctc ctggagaaat tactcgttcc ccaggaaggt tctcttgtgc agctggctgc
     1261 ccttcgtgta ttgaatcagg tgccttccga cagcgtgagc agattcctga tacagcagtg
     1321 gccggtgctg accaaagaaa tacgggacga ggccatcggg gtattggttt caaaaccgga
     1381 aaggatcgcc ctgctgattg atgcgctgga aaaaggtaaa atattaacca gcagcgtgag
     1441 ctttgaccgg agcgtccagc tgatgatgat gaaagacgat aacctgcgca accgcgcgcg
     1501 ggctttgttt acgaaaaacg aaacggaggc gaaagaaata aataaatctt accaggcggc
     1561 gctggaattg aacggcgatc ccctgaaagg caaacagata taccttgaga attgcgccag
     1621 atgtcatacc gttcggggaa aactgggcat ttcgtttggg cccgacctgg gcaccattca
     1681 caattggaaa aaggaggaca tactggcgaa catactcaat cccagcctct ccattgccgc
     1741 aggctatgaa ctgtgggaag cggaactgaa aaacaatgaa accgtccagg gaattattgc
     1801 cagtgaaacc acctcggcga tcacattgaa gaacagtgaa ggaaatgtca ggacgatcag
     1861 ccggcaggat atccggtctc taaaatcgct caatatctcc gccatgaccc cggggctcga
     1921 aaagaaaatt gacaaacagc aaatggcgga tatcctggct tttctccggc agaactgagg
     1981 cggataccgc tgccggtttt aatgtttcac cagaaaacag gccgcctttg aaaaaccttc
     2041 ggaagaaaat tttgcataat agacaccgga agcaagggtg tgcggaaagt tgacggtaaa
     2101 ctccggttga ttggatgtgg agctgctgat catcctgccg aacgtatcgt aaatgacaat
     2161 ggaatatact tcattggctt taagatcaat ccggccgaaa agggaaactc cgtttgtggg
     2221 gttgggatag atgcttattc ctacctgatc cgtatgacag ttatttacct ggatcgtgtt
     2281 gaaatatttc gtattaccgt cgacgtccgt ttgcttcagg cggaaataag aacttgcact
     2341 attagcctta tccgtagtga gcgaatattt ctgcgccgtg gatgaattac cggcactctt
     2401 tactgttccg gcaggtttcc aggtggtgcc gtcatcattg cgctcaatag cgaagtaatc
     2461 attgttcatt tcggatgcgg tggtccagtc taaaacaata tggtcattat tgcattgccc
     2521 ggtaaaggat aaaagattaa tgggcagggt gatgaggctg ttggtagtga taaaggttgc
     2581 catttggctg acccaatcac ctccggcgct tttaaaagcg gcg
//
LOCUS       DASYCU010000613         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5208182, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000613 DASYCU010000000
VERSION     DASYCU010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5208182"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VGM24_12320"
     CDS             complement(<1..343)
                     /locus_tag="VGM24_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEY4156213.1"
                     /translation="MFNTVTVVGVGLISGSFSLALKDHQLVKKVIGVSRTGGSAQRAL
                     DLGIIDEVLPLEEAVRQSDLIYVAIPVDATLPVMKTILDGITEKQIVADAGSTKQALC
                     EMLADHPMRERF"
     gene            complement(336..1529)
                     /locus_tag="VGM24_12325"
     CDS             complement(336..1529)
                     /locus_tag="VGM24_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY4156214.1"
                     /translation="MVTAQRLNGIGEYYFSKKLREIELLNREGKRIISLGIGSPDQPP
                     HPDVLKTLNEESVKPGVHGYQSYRGSPVLRNAMAAWYRQWYGVELDPEQEILPLAGSK
                     EGIMHICMTYINPGDLVLIPDPGYPTYRSAAGLASGQVAVYHLRAENDWYPDFGELKS
                     LLATKRAGQEGVLMFVNYPQMPTGQLPDKKRFEDLVSFAKENGILLVHDNPYSFILNE
                     EPMSLMSIAGAKEQVIELNSLSKSHNMAGWRVGMLLASRERINEVLRFKTNMDSGMFL
                     PLQLAAARALMLPASWYKSVNDTYRKRKAAVEALLDEMGCRYSRKQAGLFVWARVPEG
                     IKDGYALSDLVLYKAGVFITPGGIFGDSGNEYVRVSLCATEENIWESLARVKQADILK
                     PVQHV"
     gene            complement(1560..2399)
                     /locus_tag="VGM24_12330"
     CDS             complement(1560..2399)
                     /locus_tag="VGM24_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY4156215.1"
                     /translation="MLQQIQRVAIQGYEGSFHQEAARILFGEEIGILACARFQEVVNA
                     AADKTLCDAAVMAIENSIAGSILPNYSLLRNSDLQISGELYLQIRQNLLVNPGVTVKD
                     IREVHSHPMAILQCMDFLNRYDWKLVETEDTALSAKHIHQRKSKHIAGIASALAARLF
                     GLDILGPDIQTMQNNYTRFLVLQRKETVQPAKEANKASVTFHTDHSRGSLARVLTLIA
                     GGGINLSKLQSYPIPGSDWKYSFHADMEFDSMDQFNDVVKTIKPATSELKIYGVYKKG
                     ESV"
BASE COUNT          594 a          708 c          596 g          695 t
ORIGIN      
        1 caaaacgctc ccgcatcgga tgatccgcca gcatttcgca aagagcctgt ttggtggatc
       61 cggcatctgc tacgatctgt ttttccgtaa tcccgtccag gatcgttttc atcaccggca
      121 gcgtagcgtc caccggaatg gccacgtaga tcagatcgct ttgccttacg gcttcttcca
      181 gcggaagcac ttcatcaatg atccccaggt caagggcccg ttgtgcactg cctcccgtac
      241 gactcacccc gatcactttt ttaaccagct gatgatcttt gagagccagg ctgaaggatc
      301 cgctgatcaa tccaaccccg accacggtaa cggtattaaa catgttgaac cggttttaag
      361 atatcagcct gttttacccg cgccaggctt tcccatatat tttcttccgt tgcacataaa
      421 ctgaccctca catattcatt gcccgaatct ccgaaaatgc ctcccggagt aatgaatacc
      481 ccggccttat ataaaaccag gtcactcagg gcatatccgt cctttattcc ttccggaacc
      541 ctggcccaga caaacaggcc ggcctgtttc cgggaatagc ggcaacccat ttcatccagc
      601 agcgcttcca ccgcagcctt tcttttccgg taggtgtcgt tgaccgattt ataccaggag
      661 gcaggcagca tcagcgccct ggcggcggcc agctgaagcg gaagaaacat accgctgtcc
      721 atattcgttt tgaaacgcaa cacttcgttt atccgttccc gtgatgccag cagcatgccc
      781 acccgccacc cggccatatt gtgcgatttg ctcagggaat tcaattcgat cacctgttcc
      841 ttagccccgg caatgctcat caggctcatc ggttcctcat tcagaataaa actgtatgga
      901 ttgtcgtgca cgagcaaaat cccgttttcc ttcgcgaagg aaaccaaatc ttcaaatcgc
      961 tttttgtccg gcaattgccc ggtcggcatc tgcggataat tcacaaacat cagcacgccc
     1021 tcctgccctg ctcttttggt cgccagcaga cttttcaact ctccgaaatc cggataccag
     1081 tcgttttccg cacggagatg atagacggct acctggccgg aggccaaacc ggcagcactc
     1141 cggtaagtgg gatatcccgg atcgggaata agcaccaggt cgcccggatt aatataggtc
     1201 atacaaatat gcatgatgcc ttcctttgat ccggccagcg gcaggatttc ctgctccgga
     1261 tccagttcca cgccatacca ttgccggtac caggcagcca tcgcattcct caataccgga
     1321 gaaccccggt aactctggta accatgcacg ccgggtttta cgctttcttc attcaatgtt
     1381 tttagcacgt cgggatgtgg cggctgatcg ggactgccaa tacccaaact gataatccgt
     1441 ttcccttccc ggttgagcaa ctcgatctcc cgcagttttt tggagaaata atattctcct
     1501 attccgttta gccgttgagc agtcaccata atactgattg atctttgatg gatttcaaat
     1561 taaacacttt ctcctttttt ataaacaccg taaattttaa gctccgacgt ggccggcttt
     1621 attgttttca ctacgtcatt aaactgatcc atagaatcga attccatatc ggcatgaaag
     1681 ctgtatttcc agtcgcttcc cggaatcgga taggattgca gtttactcag gttgatccct
     1741 ccgccggcaa tcagggtgag tacccgcgcc aggctgcccc gcgagtggtc cgtatgaaaa
     1801 gtaaccgatg ccttgttggc ctctttggcc ggctgaaccg tttcttttct ctggaggacc
     1861 aggaaacggg tgtaattatt ttgcatggtc tggatgtcgg ggcctaaaat atccaggccg
     1921 aaaagccggg cagccagcgc gcttgcaatc ccggcaatat gtttgctctt tctttgatga
     1981 atgtgttttg cacttagtgc agtgtcttcc gtttcaacca gtttccagtc gtaccggttc
     2041 agaaaatcca tgcattgcag gatcgccatg ggatgggaat gcacttcccg gatatctttt
     2101 accgttactc ccggattgac caggagattc tggcggattt gcaggtaaag ttctccggat
     2161 atctgcaggt cgctgttgcg cagtaaagaa tagttgggga gaatgctgcc ggcgatggag
     2221 ttctcgatgg ccatcacggc ggcatcgcaa agggttttgt cggccgccgc gtttaccacc
     2281 tcctggaacc gggcacaggc aaggattccg atctcttccc cgaaaaggat cctggccgcc
     2341 tcctggtgaa aactgccttc atatccctgg atggccacgc gttgtatttg ctggagcata
     2401 tatggtaaaa aaaaatcccg gcttttgacc gggacgttat tgttaagatt ttagtttagt
     2461 tgtctgttaa caaaagcttt cccggtctgc tggtaaagta gaaaaaatag aatccaaaaa
     2521 aacggtatgc ttttgcgttg tgcatatgag ttgtcaaaaa taattgattc tgctgatcct
     2581 gcaaattttt att
//
LOCUS       DASYCU010000614         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1732006, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000614 DASYCU010000000
VERSION     DASYCU010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732006"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="bioA"
                     /locus_tag="VGM24_12335"
     CDS             complement(<1..1023)
                     /gene="bioA"
                     /locus_tag="VGM24_12335"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY4156216.1"
                     /translation="MNQMNQPSLSERDQQVIWHPYTQHQQADLPIPIVRGEAALLFDE
                     AGNAYIDAISSWWVNIHGHAHPYIAEKIYRQAAKLEHVIFTGFTHEPAVQLAERLLKI
                     LPGNLEKIFYSDNGSTAVEVALKMGIQYWKNKQQPKRNKILAFRHAYHGDTFGAMSVS
                     SRGLFTEAFHDFLFDVLFIDTPDDRNAGELKTFIESRKEEIACFIYEPLLQAAGGMRI
                     YPASGLDALLKTARENQILCIADEVLTGFGRTGTLFATESLSHQADIMCLSKGLTGGT
                     LALGATACSQEIFEAFLSDDRRHTLFHGHSYTANPIACAAGLASLDLLQEKACMDQIR
                     SLTEKNN"
     gene            complement(1020..1967)
                     /gene="queG"
                     /locus_tag="VGM24_12340"
     CDS             complement(1020..1967)
                     /gene="queG"
                     /locus_tag="VGM24_12340"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY4156217.1"
                     /translation="MKNLAPHTRLIRSLAASAGFDHCGIAAARPLDDDARKLEQWLHH
                     GLHGGMQYMEKHFDLRVNPYKLLPGAKSVISFLLNYYPEEKQDEGVPKISRYAYGKDY
                     HEVIRPKLNFILKRMKEEIGDFQGRGFVDSAPVMERSWAVRSGLGWVGKNGNLIHKKQ
                     GSYFFIATLIVDIELEYDAPYTRDFCGDCTRCIDACPTGAIHDDKVVDGSQCISYFTI
                     ELRELLIPDTAKGKMDNWMFGCDVCQEVCPWNRFSRPTAEVDFSPVPELLHYNQKDWE
                     EITEEGFKKIFSASPLKRAKYQGIRRNLLFLKQGNTNSA"
     gene            2217..>2739
                     /locus_tag="VGM24_12345"
     CDS             2217..>2739
                     /locus_tag="VGM24_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEY4156218.1"
                     /translation="MKKYVTLFLALSAFFNLRAQSYPASAIPDSLKQNADMVVRLDER
                     VTEIKSPGRIIEREHHVYTVLNENADRYATYKTGYDKFTSINYVNATLYDASGKELKH
                     FRKKDMQDFASESAESLVTDVRYKIASFSCHTYPYTVDFEEEDEIESALRIHGWSLSR
                     SVKISLECSRYSII"
BASE COUNT          671 a          687 c          677 g          704 t
ORIGIN      
        1 attatttttt tccgtcaggg accgtatctg atccatacag gctttttcct gcagcagatc
       61 caggctggcc agcccggccg cgcaggcgat cggatttgcc gtatacgaat gcccgtgaaa
      121 caaagtgtgc cggcggtcat cactcagaaa ggcttcgaag atttcctgcg agcaggcggt
      181 ggcgcccagc gccagggtcc ccccggtcag ccctttgctc aggcacatga tgtctgcctg
      241 gtgggaaagg gattccgttg caaaaagggt tcctgttctg ccgaagccgg taagtacctc
      301 gtcggcaata caaaggatct ggttttccct ggcagttttg agtaaggcat ccagaccgga
      361 agccgggtat atcctcattc ctcctgcggc ctgcagcagg ggctcataaa taaaacaggc
      421 aatttcctct ttccgggatt cgataaaggt tttcagttcg cccgcattgc gatcgtccgg
      481 cgtatcaata aacagaacat caaataagaa atcatgaaat gcctcggtaa aaagaccgcg
      541 gctgctaacg ctcattgcac cgaatgtatc gccgtggtag gcatggcgaa aagccaggat
      601 tttatttctt ttgggttgct gtttgttttt ccagtactgg atacccattt tgagggccac
      661 ttccaccgcc gtcgacccgt tgtcggaata aaatattttc tccaaatttc ccggaaggat
      721 ttttagcaac cgttccgcca gttgcacggc cggctcatgc gtaaaaccgg taaagatcac
      781 atgttccagt ttcgccgcct gccggtaaat cttttcagca atatacggat gtgcatgacc
      841 gtgaatattc acccaccagc tgctgatggc gtcaatatag gcattacccg cctcgtcaaa
      901 aaggagggcc gcttctccgc gtacgatggg tataggcagg tcagcctgct gatgctgggt
      961 atagggatgc cagattacct gctggtcccg ctcactgagt gaaggctggt tcatttgatt
     1021 catgcggagt tggtatttcc ctgtttcaga aaaagaagat ttctccggat cccctggtat
     1081 tttgcacgtt tcaggggaga ggcggagaaa atttttttaa atccttcttc cgttatttct
     1141 tcccagtctt tctgattata atgaagtaat tcgggaacgg gcgagaagtc cacttccgcc
     1201 gttggcctgg agaaccggtt ccacgggcaa acctcctggc agacatcaca cccgaacatc
     1261 cagttgtcca tttttccttt cgcggtatcc gggatcagga gttccctgag ctcgatggta
     1321 aaataggaaa tgcactggct gccgtccacg accttgtcgt catggatggc gccggtcggg
     1381 caggcatcaa tgcaacgcgt gcaatcgccg cagaaatccc gggtatatgg agcatcgtat
     1441 tccagttcaa tatccacgat cagcgtagca ataaaaaaat aagaaccctg ttttttgtga
     1501 atgaggttgc cgttttttcc gacccagccc aggccgcttc gtactgccca ggacctttcc
     1561 ataaccgggg cgctgtccac gaaccccctt ccctgaaaat cgccgatttc ctctttcatc
     1621 cgtttcagga tgaaattcag tttcggccgg atcacctcat gataatcctt cccataggca
     1681 tagcgggaga ttttgggaac gccttcatcc tgtttttctt ccggataata attcagaaga
     1741 aagctgatca cagactttgc cccgggaagg agcttgtagg ggtttactct taagtcgaag
     1801 tgtttttcca tgtattgcat gccgccatgc agtccgtgat gcagccattg ctcgagtttt
     1861 cgcgcatcat cgtcaagcgg acgggctgcg gctattccgc aatggtcaaa tcccgcggaa
     1921 gcggccagcg aacggatgag acgggtatga ggtgccagat tcttcatgca aaaatcaaat
     1981 cattgacggg gaggcaaaat ttttttattt ttaacgcgtt taacaagttt ataacagccc
     2041 ctgaacaaag ttaccgctct gcacaggaaa agcccgagat aaaatcagca gttggagcag
     2101 ccgctgattt tgaacttttc ccggattaat cgctatggac tcaccgggtg ctgaattcgg
     2161 caagggactt ccttcaatca tcgggaagaa gtcagcctta aaatgaaatc cggatcatga
     2221 agaaatatgt gaccttattc ctggccttat ccgctttctt taatctgaga gcgcaatctt
     2281 atccggcttc cgctatcccc gattcgctga agcaaaatgc cgatatggtt gtccggctgg
     2341 atgaacgggt aactgaaatt aaatcccccg gccgtatcat agaacgcgaa caccatgtat
     2401 acacggtgct gaatgaaaat gccgaccggt atgccacgta taagaccggg tatgataaat
     2461 ttacgtcgat caactatgtc aatgccaccc tttacgatgc cagcggaaaa gagctgaaac
     2521 atttcaggaa aaaggatatg caggattttg cgtccgaaag cgcggagtcc ctggttaccg
     2581 acgtccggta caaaatagcc agtttcagct gtcatactta tccgtatacg gtagattttg
     2641 aagaagagga cgaaatagag agcgcgctgc ggattcatgg ctggtctcta tctagatccg
     2701 ttaagatttc actggaatgc agccggtaca gcattatcg
//
LOCUS       DASYCU010000615         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2887685, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000615 DASYCU010000000
VERSION     DASYCU010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887685"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGM24_12350"
     CDS             <1..312
                     /locus_tag="VGM24_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEY4156219.1"
                     /translation="IELTSHEELHLVIGMVKDKAIENILRHLPQTANYYFTRAQIPRA
                     LPETELAAKAETLGLKGKNFPDVHQALQAAVTRARPKDLILVCGSVFLAGEVNLREIV
                     F"
     gene            complement(293..844)
                     /locus_tag="VGM24_12355"
     CDS             complement(293..844)
                     /locus_tag="VGM24_12355"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY4156220.1"
                     /translation="MKDLRWKKLSSRYLLRETWGTVRRDVCETREGKIIDPYFVYEFP
                     TWVTAVAVTADHKIILERQYRYALDTVNYEIPGGCVDDTDESLEAAIARELMEETGYR
                     FDHYEYLGKTSANPSTNNNWMHMYLATGGRYEREQQLDHNEEIEVYLFSVDEVKKMLD
                     ENQIIQSMHVTALYYGLPKIQFP"
     gene            complement(881..1405)
                     /locus_tag="VGM24_12360"
     CDS             complement(881..1405)
                     /locus_tag="VGM24_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HEY4156221.1"
                     /translation="MNNPSYPIGRYQPLPYSDEQKKIWLNQIRQLPVELEEVIRNLDA
                     AQLHSPYREGGWTVHQLVHHIADSHMNAYVRFKLGLTEHQPVLKSYEEKLWAELADVE
                     LLPVNVSITLLHALHSRWYEAIKNLTELQWLRTVIYPSTQKETTLWYMLGMYAWHGKH
                     HVAHITVFREKNGL"
     gene            complement(1455..2219)
                     /locus_tag="VGM24_12365"
     CDS             complement(1455..2219)
                     /locus_tag="VGM24_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4156222.1"
                     /translation="MDKEVIIKIDQLTKNYGEFEAVKGISFEVYQGEIFGLLGPNGAG
                     KSTTLEIIETLRKKTSGKVMVDGLDLDKSPEDIKKIIGVQLQTSGYYPNLSLTELLIL
                     FGGLYNEHIRPADFLAKVNLTDKAKAKYKELSGGQKQRFSIATTLINKPKIIFLDEPT
                     TGLDPQARRNLWDLILEIRSSGTTVIITTHYMDEAEVLCDRVAIIDSGKIIAMDSPDQ
                     LIDNLVATGFERPREVKKANLEDVFIQLTGKAWREE"
     gene            complement(2262..2774)
                     /locus_tag="VGM24_12370"
     CDS             complement(2262..2774)
                     /locus_tag="VGM24_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY4156223.1"
                     /translation="MRRDLPKLSFFFLVFIPARFAWTTGIPRPDIPVQQEKLPVKESR
                     LLFSMVTDILTYVNEDRKQQGLAPLTLNEVESDIAARHSQDMASGRVPFGHSGINTRA
                     KAIRKALGAIRSVGENVASGPMNAREVVDGWLHSPGHKRNIEGDFTLTGIGYAPDKKG
                     NIFFTQIFSK"
     gene            2962..>3238
                     /locus_tag="VGM24_12375"
     CDS             2962..>3238
                     /locus_tag="VGM24_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156224.1"
                     /translation="MKVFKFGGASVSNTERVRRLPDILRSFAKEKLLIVISAMGKTTN
                     ALEKVATAFYSGNTEEAVRQFNLIKKDHLEMAGALLSSGSPGGPSRLP"
BASE COUNT          772 a          827 c          775 g          864 t
ORIGIN      
        1 attgaattga cttcccacga agaattacat ctggtcattg gcatggtgaa ggataaagcg
       61 attgaaaaca ttctccggca tctgcctcaa accgcaaatt attattttac ccgggcacag
      121 atcccgcgcg ccttacccga aacggaactg gcggcaaagg cggagaccct cggcctgaaa
      181 ggaaaaaatt tcccggatgt gcatcaggcc ctgcaagccg ctgtcacccg cgcgcgtccg
      241 aaagacctca tcctggtctg cggaagcgtt tttcttgcag gagaagtgaa tctcagggaa
      301 attgtatttt aggtaatccg tagtataatg ccgtcacgtg catactctga atgatctggt
      361 tttcatccag catttttttg acctcatcca ctgaaaaaag gtatacttcg atttcttcat
      421 tgtggtccag ctgctgctct ctttcatagc ggccgccggt agccaggtac atgtgcatcc
      481 agttattatt ggtggacggg ttggcagacg ttttgccaag gtattcataa tgatcgaagc
      541 ggtaacccgt ttcttccatc agttcccgcg cgatagccgc ttccagggac tcatccgtat
      601 cgtccacaca gcctccgggg atctcgtaat taaccgtatc cagcgcataa cggtactgtc
      661 tttccagaat aattttatga tcggccgtaa cggcaaccgc cgtcacccag gtgggaaatt
      721 catacacaaa ataagggtca atgatcttgc cttcccgggt ttcgcataca tctctcctga
      781 ccgtacccca ggtttcgcgc agcaggtacc gggaggatag ttttttccat cggagatcct
      841 tcataggccg atatccattt tcaggtgaat tttatttttg ctaaagacca tttttttcac
      901 ggaaaacagt aatatgcgcc acatgatgtt ttccatgcca ggcatacatg ccgagcatat
      961 accagagcgt ggtttctttt tgggtggaag gatagatcac ggtgcggagc cactgcaatt
     1021 cagtcaggtt tttaatggct tcataccagc gggaatgcaa ggcatgcagc agggtgatgg
     1081 acacattcac cggaagcagc tccacgtcag cgagttcagc ccaaagtttc tcctcgtacg
     1141 atttcaggac gggctgatgc tcggttagcc cgagtttaaa ccggacatag gcgttcatat
     1201 ggctgtcggc aatatgatgg accagctggt ggacggtcca gccgccttcc cggtaggggc
     1261 tgtggagttg cgctgcatcc aggttgcgga tgacttcttc cagttccaca ggaagctgcc
     1321 ggatctggtt cagccagatt ttcttctgct catcagaata aggcagcggt tgatatcttc
     1381 cgatgggata agagggatta ttcatgccgg ttgatcatta ataattgatg gttaatagtg
     1441 gacaaacgtt gatttcactc ctccctccag gctttgccgg taagttgaat aaacacatct
     1501 tcgagatttg ccttcttcac ttccctgggt ctttcgaaac cggtggccac caggttgtca
     1561 attaattgat cgggagaatc catggcgatg attttacctg aatcaatgat tgcgacccgg
     1621 tcgcacaata cctccgcctc atccatataa tgcgtggtta tgatcacggt cgtgcccgaa
     1681 gagcggattt ccaggatcag atcccagaga tttcttcttg cctgcggatc gagcccggtg
     1741 gtgggttcat ccaggaagat aatctttggt ttattgatca gcgtcgtagc gatggaaaag
     1801 cgttgcttct gccccccgct taattccttg tatttggctt tcgctttgtc cgtcagattc
     1861 acttttgcca gaaaatctgc cggcctgatg tgttcattgt acagtccgcc gaaaaggatc
     1921 agcaattcag tcaggctgag gttgggataa tacccggagg tctgcaactg gaccccgatg
     1981 atctttttga tgtcttccgg cgatttatcg agatccaacc catccaccat cactttcccg
     2041 gaggtttttt ttcgaagcgt ttcaataatt tccagggtgg tggatttacc ggccccattg
     2101 ggacccagca ggccaaagat ttctccctgg tacacctcaa aactgattcc tttaaccgcc
     2161 tcaaactcac cataattttt tgtaagctga tcgattttta ttatgacctc tttgtccatt
     2221 aggaggcaag ttacggcagc cgcgctattt aaccccgtcc tttacttcga aaaaatctgg
     2281 gtaaaaaaaa tattcccttt tttatccggg gcgtatccga tccccgtaag ggtaaagtcc
     2341 ccctcaatat tgcgtttatg tcccggactg tgcaaccagc catccactac ctcccgggca
     2401 ttcatcggcc cggacgccac gttctcgccc accgaacgga ttgcacccag tgccttccgg
     2461 atggcctttg cccgagtatt tattccgcta tgtccgaagg ggaccctgcc gctggccata
     2521 tcctggctat gccgggcagc aatatccgat tccacctcat taagggtaag cggtgccagc
     2581 ccctgttgtt tgcggtcttc attgacatag gttaatatgt cagtcaccat ggaaaaaagt
     2641 agccgggatt ccttgaccgg aagcttttcc tgctgaaccg ggatatcagg gcggggaata
     2701 ccggttgtcc acgcaaaacg ggccgggatg aataccagga agaaaaaaga aagtttagga
     2761 agatcgcggc gcatagagaa agatatattt atttaaaata caaataattt accggcaggc
     2821 ggatgggctc caatcttttt aacatagtta tcacgatccg gttccgggca cattatcttt
     2881 gcgccggaac gctgaacgca gcctgcggca tgcttatttc gatcttccgg cgttccgcat
     2941 taaagaagta taaaacctga aatgaaagtt ttcaaattcg gcggcgccag cgtcagcaat
     3001 acagaaaggg tccgccgtct tccggacatc ctgcgcagct tcgccaaaga aaaattgctg
     3061 atcgttattt cggctatggg taaaacaacc aatgccctgg aaaaagtagc cactgctttt
     3121 tattccggaa atacggaaga agccgttcgt caattcaacc tgataaaaaa ggatcacctg
     3181 gagatggccg gcgccctgct ttcttccggc tcccccggag ggccttcccg cttacctg
//
LOCUS       DASYCU010000616         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1442387, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000616 DASYCU010000000
VERSION     DASYCU010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442387"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VGM24_12380"
     CDS             <1..640
                     /locus_tag="VGM24_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEY4156225.1"
                     /translation="AKMNHHVVMTPWDYCYLDLYQGETTAEPPTYGMCRLSDSYNYDP
                     VPDSVDEKYILGGQGNLWTERVPNFRQVEYMTWPRALALAEVYWSPKASRNWDDFIKR
                     MEVQFERFDAADIKYARSAYNVIFTPVRNAGREFSVKLSTEIKGLDIYYTFDDTNPDS
                     DYPKYQGEPIQFPFGATQLNVVTYRDGKPVGKQINIKKEELASRLNARRHEY"
     gene            complement(637..1818)
                     /locus_tag="VGM24_12385"
     CDS             complement(637..1818)
                     /locus_tag="VGM24_12385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4156226.1"
                     /translation="MKHQWKPLMLVAYVFIFTGAIICMNDVLLPSLKDFFHLSYVQAS
                     FVQQSFYLVYLIFPIPIAYYISRYGYKTGLITALLICSFGSLLFIPAYFSASYAICLT
                     ALFVISIGVTLINVAANPLAALLGDPSGAHIRVNFVQLFSRIGYSLTPILATRLIYGN
                     HNTVSFHIPYLILGVGTLLLAIFIVFSALPSFKPEVLKGFSFFKILKESRQYPQLFWG
                     AIIMFFYVGAEACTAGFFISYLREVSGFNPAQTARFLTYYYIAATLVSLVGIYLLRII
                     SAGRLVALFGVGMVSMYLLAALTSSALNPYYMIALGVFISIMFPSLFSLGIEGVGSFT
                     EKGSALIKIAVVGGAVFPPLQGMIADAKGVQLSYLVPCCCFVLIVIYGIFCDRRYLRG
                     R"
     gene            complement(1837..>2091)
                     /locus_tag="VGM24_12390"
     CDS             complement(1837..>2091)
                     /locus_tag="VGM24_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156227.1"
                     /translation="TGKRYFAHNGNDPGAFEPAVLFWFEFKPGKSPVWIPHRIDDNSG
                     VGLQVVVKDMNRDGFPDIIIANKKGVFLFERIPHPSRAGD"
BASE COUNT          636 a          486 c          541 g          428 t
ORIGIN      
        1 agcaaaaatg aatcaccacg tcgtgatgac gccctgggat tactgttacc tggacttgta
       61 ccagggagaa acgacagccg aaccgcccac ttacggaatg tgccgtttga gcgattccta
      121 taattatgat cccgtaccgg acagtgtgga tgaaaaatat attttaggag gacagggaaa
      181 tctgtggaca gaaagagtgc ccaatttccg tcaggtggaa tacatgacct ggccccgcgc
      241 gctcgccctc gcggaagttt actggagccc gaaagccagc cgcaactggg atgattttat
      301 taagcggatg gaagtccagt ttgagcgctt cgatgcggcg gatatcaagt atgcccgcag
      361 cgcctacaat gtcatcttca ccccggtccg gaacgccggc cgggaatttt ccgtgaaact
      421 gtcaacggaa ataaaagggc tcgatattta ctatacgttc gacgatacca atccggacag
      481 cgactatccg aaataccagg gcgagccgat acaattccca ttcggcgcca ctcaattaaa
      541 cgtagttact taccgcgatg gtaaacccgt cggcaaacag ataaacatca aaaaagagga
      601 actggcttcc aggctgaatg cccggcggca tgaatattaa cggccccgga ggtatcttct
      661 gtcgcagaat attccgtaaa tgacgatgag tacaaagcaa cagcagggaa cgagataaga
      721 aagctgaact ccttttgcat ctgcaatcat gccctggagg ggagggaaaa cagcgcctcc
      781 caccacggct attttgatca gcgcggaacc cttctcggta aaacttccaa cgccttcaat
      841 acccagactg aacagggaag ggaacatgat ggatatgaac acccccaggg cgatcatata
      901 ataaggattc agggcggagc tggtaagggc ggctaacagg tacatcgata ccatgccgac
      961 accgaacagc gcgaccagcc ggccggcgga tatgattcgt aaaagataga ttccaaccag
     1021 ggaaaccagt gtagctgcaa tataatagta agtgaggaac ctggccgttt gtgcaggatt
     1081 aaatccggaa acctcccgca ggtagctgat gaaaaatccg gcggtgcagg cttccgcgcc
     1141 cacataaaaa aacatgatga tagcgcccca gaacagctgc gggtactgcc tggattcctt
     1201 caggatctta aaaaaagaaa aacctttgag cacctccggt ttgaaagacg gaagggcgga
     1261 gaagacgatg aaaatcgcga gaagcaaagt tccgacgccc aggatcaggt aaggaatatg
     1321 gaaggatacc gtattgtggt tgccgtaaat aagtctggtg gcaaggatcg gggtcaggga
     1381 ataaccgatc cggctgaaca gctgaacgaa attcactctt atatgggcgc cggacggatc
     1441 accgagcaag gcggctaacg ggttggcggc aacgttaata agagtcaccc caatggagat
     1501 cacaaaaaga gccgttaagc aaatggcata ggaagcacta aaataggccg gaatgaaaag
     1561 taaggatcca aaactgcaga tcagcagcgc cgtgatcaaa ccggtcttgt atccatagcg
     1621 tgaaatatag taagcaatcg gtatggggaa aatcaggtaa accaggtaga aggattgctg
     1681 gacaaaagag gcctggacgt aagaaagatg aaaaaaatct tttagtgacg gaaggagcac
     1741 atcattcatg caaatgatcg caccggtgaa tatgaataca taggcgacga gcattaacgg
     1801 tttccactga tgtttcattt gattgtgttg caatgattaa tctccggccc gggaagggtg
     1861 gggaattctt tcaaaaagaa aaacgccttt tttatttgct ataataatgt ccgggaatcc
     1921 gtcccggttc atatccttca cgaccacctg caggcccaca cctgaattat catcgatccg
     1981 gtgcggtatc cataccggtg atttaccggg tttaaattca aaccaaaata aaaccgccgg
     2041 ttcgaatgcg ccgggatcat tgccattatg ggcaaaataa cgtttcccgg t
//
LOCUS       DASYCU010000617         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3613105, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000617 DASYCU010000000
VERSION     DASYCU010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3613105"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VGM24_12395"
     CDS             complement(<1..662)
                     /locus_tag="VGM24_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156228.1"
                     /translation="MTHSDTYKEFEARKNRLASFYTFLICTSLFLLFFLVSWTLPFNP
                     PQPVEEGLEVNLGNSDQGLGTDQPYLPGDPSAQNQQKYTPPSPVAPQKEALKSVETDD
                     ANPDPAPAVKQPPVTKPEATKIPEKDLAVKKTRVTKQTSVNPNPTPPRPKAVFQGVNG
                     SGNGGNDADHYKPGGNQGIAGGKGDQGAPGGDPNSTNYKGGGHGSGVAILRGLQGRSW
                     RSL"
     gene            complement(746..>1555)
                     /locus_tag="VGM24_12400"
     CDS             complement(746..>1555)
                     /locus_tag="VGM24_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY4156229.1"
                     /translation="NCCMTYHCPAARNLSLGRWECPVPVSPACNANCVGCISFQPQGE
                     TIHSTQDRLTFRPTAEEIVEFTVPHLQTAPYPIVSFGQGCEGEPLLMWETIREAIIEI
                     RKHTKRGSININTNGSKPAAVQALTDAGLNSIRVSMNSARKHIYEAYYRPNNYVFEDI
                     VESLCVMREAGGWTSINYFVFPGMTDSTEEYEALRKLIRTTGLAMIQWRNFNIDPDWY
                     LGKIGSSETGEAMGIRNLMNLLKEEFPLLKFGYFNPPMERIRGNFEKDFAS"
BASE COUNT          347 a          397 c          398 g          413 t
ORIGIN      
        1 aggctgcgcc aggaccttcc ctgcagaccc cgcaggatag cgacgccgga tccgtgtccg
       61 ccgcccttat aattggttga attcggatct cctccgggtg ctccctgatc ccctttgccg
      121 cctgcaatgc cctgattccc gccgggttta taatggtcag catcgtttcc gccattgccg
      181 gaaccgttta ccccctgaaa tacggctttg ggacggggag gcgtgggatt tggattaaca
      241 gaagtctgtt ttgtcaccct tgttttcttt acggccaggt ctttctccgg aatttttgtg
      301 gcttcaggtt tggtgaccgg cggctgtttt actgcaggag ccggatcggg atttgcatca
      361 tcggtctcca cactttttaa agcctctttt tgtggcgcaa ccggactcgg tggcgtatat
      421 ttttgctgat tttgagcaga tggatctcca ggcaggtatg gctggtcagt cccaagcccc
      481 tggtcgctgt tccccagatt tacttccaga ccttcttcca ccggttgggg cggattaaag
      541 ggcagggtcc aggataccag gaaaaaaagc agaaacagac tggtacagat gaggaaggta
      601 taaaaagaag ccagccggtt tttccgggct tcaaactcct tatatgtatc ggaatgcgtc
      661 acggtacgaa gttagttcaa acctttggga atatacattc tggttcgggg actgccggat
      721 gaaagtaaac ggcggacctt gcgtttcaag atgcaaaatc tttttcaaaa ttgccccgga
      781 tccgctccat cggcggattg aaataaccaa attttaacag cgggaattcc tcctttaaaa
      841 gattcatcag gtttctgatg cccatcgctt cgccggtttc cgaactgccg atttttccca
      901 gataccaatc cggatcaata ttaaaattcc gccactggat catggccagg ccagtcgtcc
      961 gaatgagttt tctcagcgct tcatattctt cggtactgtc cgtcatcccg ggaaaaacaa
     1021 aataattgat ggatgtccat ccgccggcct cccgcattac gcagaggctt tccacgatgt
     1081 cttcaaatac ataattattc ggccggtagt aagcttcata aatatgtttt cttgccgaat
     1141 tcatgctcac ccggattgaa ttcagccccg catcagttaa agcctgaaca gccgcgggtt
     1201 tggatccgtt cgtgttgatg ttgatgctgc ccctttttgt atgtttccgg atttcgatga
     1261 tggcttcgcg aatggtttcc cacatgagca ggggttctcc ctcacagccc tgaccgaaac
     1321 tgacaatggg atagggggcc gtttgcaaat gaggaacagt aaattccacg atctcttctg
     1381 cggtcggtct gaacgtgagc cggtcctgcg tggaatgaat ggtttctccc tggggctgaa
     1441 aagaaatgca acccacacag ttggcattgc aggcagggga cacgggaacc ggacattccc
     1501 aacggcccag ggaaaggttg cgcgcggcag gacaatgata ggtcatgcag caatt
//
LOCUS       DASYCU010000618         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4046516, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000618 DASYCU010000000
VERSION     DASYCU010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4046516"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VGM24_12405"
     CDS             <1..242
                     /locus_tag="VGM24_12405"
                     /inference="COORDINATES: protein motif:HMM:NF025665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEY4156230.1"
                     /translation="SWDNTARRGESAFLFKNATPQKYQEWIQFHADHFKPPSAEENFL
                     FINAWNEWAEGNHLEPCQRWGSQYLEATRKVFGHD"
     gene            235..1194
                     /locus_tag="VGM24_12410"
     CDS             235..1194
                     /locus_tag="VGM24_12410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4156231.1"
                     /translation="MIEVSVIVPNFNHAKYLRKRMDSILSQTYREFELIILDDCSSDD
                     SKTIIEEYRGNPLVSHIVYNDANSGSTFRQWNKGLDLARGELIWIAESDDWCEPTFLE
                     KTVQKMAEYPTAGLVYTQSMEFEETTGKSFLSFQDHSLFSETFLRSYFAKGRSEIMSK
                     LAYENTIPNASAVLFRRTIYEKAGGADETMKLCGDWFLWTRMLFHSDVYFIAEPLNYF
                     RLTAASARSRFSPEQTFPERMKVLHYIKEHHVKAGDKAAMKLIQRLINSYRLMEIKKP
                     IQTIWAQKKLIWFPEGKIALALFLSLFHRVFRKMKRHPASALL"
     gene            1199..>2363
                     /locus_tag="VGM24_12415"
     CDS             1199..>2363
                     /locus_tag="VGM24_12415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4156232.1"
                     /translation="MKLFYFTSSYPYGLGERWKTNELKELVHHFDQITVVPYSYEHNF
                     DNPKKLPEGVALSGPLFRDSSLSLSRLKAPVQILFSRHRKIFISEFFKKRVYRKKSHF
                     VSWLMATLNALRLLDHPVIRRILQDGDDQTILYFFWGKGSAEILPFIDTTRFYRTFVR
                     MHRYDLFETENNNYIPYRSFLLNKADRIAPSSEAGRIHLTRLYPGFQDKIFVYRMGTN
                     GNGKRSSPSADPVFRIVSCSFLSPVKRVDIMVESLRYVDFPVLWRHIGDGKQRDQIEE
                     LIGRYSLQEKFILEGMIDSDQVLDFYTGNAIDLFVNTSASEGVPFSIMEAFSVGIPVM
                     ATQVGGTAEIVDESVGNILPAGLDPRLLARYLKEYYSLPEAEKVQKRNNAYRRY"
BASE COUNT          622 a          531 c          580 g          630 t
ORIGIN      
        1 catcctggga caatacggcg cggagaggcg aatctgcgtt cttatttaag aatgcaacac
       61 cgcaaaagta ccaggagtgg attcagtttc atgcggatca tttcaagccg ccgtcggcag
      121 aggagaattt tttgtttatc aatgcctgga atgaatgggc ggagggaaat catctcgaac
      181 cctgccagcg ctggggttca caatatttag aggccacgag gaaagttttt gggcatgatt
      241 gaggtgtcgg tcatagtccc caattttaac cacgcaaaat acctgagaaa gagaatggat
      301 tccatcctgt cgcagactta ccgggaattt gaattgatca ttcttgatga ttgttcttcg
      361 gatgacagta aaacaattat agaagagtac cggggcaatc cgcttgtttc tcacatcgtt
      421 tacaatgacg caaacagcgg ttctaccttc cggcagtgga ataagggtct cgacctggcc
      481 cgcggagagt tgatctggat agccgagagc gatgattggt gcgagccgac ctttctggaa
      541 aagaccgtgc aaaagatggc agaatatccg acggccggcc tggtttacac gcaaagcatg
      601 gaatttgagg aaacgacagg aaaaagcttt ctgagtttcc aggaccattc cctgttttcc
      661 gaaacttttc tccggtctta ttttgccaaa ggaagaagcg agatcatgag taaactggcc
      721 tatgagaaca ctatcccgaa tgccagtgcc gttttattca gaaggacgat ctatgaaaag
      781 gcgggtggcg cagatgaaac catgaagctc tgcggtgatt ggtttttgtg gaccaggatg
      841 ctgtttcatt cggatgttta ttttattgcg gaaccactca attatttcag gctcaccgcc
      901 gcttccgcca gatcaagatt ttcgccggaa cagacctttc ccgaacgaat gaaagtcctg
      961 cattatatta aggaacacca tgtaaaagcc ggggacaagg cggccatgaa actgattcag
     1021 cggttgatta attcttaccg gcttatggaa ataaaaaagc cgattcagac catatgggcc
     1081 caaaaaaaac tgatttggtt ccccgaagga aaaattgcac tcgccctttt cctttccttg
     1141 tttcaccggg ttttccggaa gatgaaaaga catcccgcat ctgcattgct gtagatcaat
     1201 gaaattattt tattttactt cttcctatcc ttatggcctt ggcgaacgct ggaaaacaaa
     1261 tgagctgaaa gaactggttc atcattttga tcagatcacg gtggtcccct attcgtacga
     1321 gcataatttc gataatccga aaaagcttcc cgaaggggtg gctttatccg ggcctttgtt
     1381 ccgggactcc tctctttctt taagccggtt aaaggctccg gttcagatct tattcagccg
     1441 gcaccggaag atctttatat cggaattttt taaaaaaagg gtttacagga aaaagagtca
     1501 tttcgtaagc tggttaatgg caaccctgaa tgcgcttcgc ttgctggacc atccggttat
     1561 ccggcggatc ctgcaggacg gggacgatca gaccatctta tattttttct ggggtaaggg
     1621 atctgcggag atccttccct tcatagatac cacccggttc tacagaacat ttgtgcggat
     1681 gcaccggtat gacctttttg aaacggaaaa caacaattac attccttacc gttcattcct
     1741 gctgaacaag gccgatcgga ttgctccttc ttcggaggcc ggaaggatcc atttaacccg
     1801 gctttatccc ggatttcagg ataaaatttt tgtataccgg atgggaacga atggaaatgg
     1861 gaaaagatcg tcaccctccg cagatcctgt tttcaggatc gtcagctgtt cctttctttc
     1921 tccggttaaa cgcgtggata tcatggttga aagtttgcgg tatgtcgact tccctgtttt
     1981 atggcgccat atcggggacg gcaaacagcg cgaccagata gaggaactga tcggccggta
     2041 ctccctgcag gaaaaattta ttctggaggg catgattgac tccgatcagg tgcttgattt
     2101 ttataccgga aacgccattg acctgtttgt aaatacgagt gccagtgagg gggttccctt
     2161 ctcgatcatg gaagcgttct ctgtcggaat tcccgtaatg gccacccaag tgggcggaac
     2221 cgctgaaatt gtagatgaat cagtcggaaa cattttgccc gccggcctgg atccccggtt
     2281 gctggcccgg tatttaaaag aatactactc tttaccggag gctgaaaaag ttcaaaagag
     2341 aaacaatgcc taccggagat ata
//
LOCUS       DASYCU010000619         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_857805, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000619 DASYCU010000000
VERSION     DASYCU010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857805"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VGM24_12420"
     CDS             <1..335
                     /locus_tag="VGM24_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY4156233.1"
                     /translation="MNIYVSNLSFNVTDEDLNEYFAEYGEVSSAKVILDKFTQKSRGF
                     AFVEMTDEEAAKKAIQELDGASVDGRTINVSVAKPREERTGGGAGNRAGGGGGNFQKR
                     NNFSNSRY"
     gene            931..>1532
                     /locus_tag="VGM24_12425"
     CDS             931..>1532
                     /locus_tag="VGM24_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY4156234.1"
                     /translation="MPIEAGMASPPLTAKDFTTDQEVRWCPGCGDYSILAQVQKVMPQ
                     LGIPRENIVIISGIGCNSRFPYYMNTYGMHSIHGRATAIASGLKASRPELSVWVVTGD
                     GDSLSIGGNHTIHLLRRNFDLNILLFNNQIYGLTKGQYSPTSETDKLTKSTPFGSIDH
                     PFNPLALAMGADASFVARSMDRDPKHLQQMLMRSHQHRGSS"
BASE COUNT          392 a          389 c          362 g          389 t
ORIGIN      
        1 gtatgaacat ttatgtttca aatttaagct tcaacgtaac cgatgaagac ttaaacgagt
       61 attttgcaga gtacggtgaa gtttcatctg ctaaagttat tcttgacaaa ttcacgcaga
      121 aatctcgcgg atttgccttt gttgaaatga cagacgaaga agcggctaaa aaagcaatcc
      181 aggaactgga tggcgcttca gtggatggca gaacgatcaa cgtttctgta gctaagcccc
      241 gtgaagaacg cacaggcggc ggtgctggta acagggccgg cggcggcggt ggtaatttcc
      301 agaaaagaaa taacttcagc aacagcagat attaatcgct tttaattcaa agccgttgat
      361 ccggagtttt ccgggtcaac ggcttttttg ttgttgggca gatggtttca gggcggatcg
      421 ggattaccaa tgacccctcc tccatctcca tccattttta tgaccttccc aatggccccc
      481 gtgccagctg ccgccggtcc ggcgccgttc ccagcgtcct tcctgccagc gatagctccc
      541 ccctgcccat atccagtcgc cgctaatcca aacataatcg gggcccggag caggagtcct
      601 gctgtaaacg acatcatgag gacgggtagc tacgacctct gtgctacagc cccccaggcc
      661 gatcaatatg cctgcacatg ctgccaataa ccattttttc attttgctgt tatttgattg
      721 aattaaagca aaacctgttc tccttttgga ctgaagagta aggatcaaaa agtaaaaaac
      781 tggttaccat tgatttataa gaattttatg gaccgcatct tttttacttt taacattgta
      841 cccttgcccc ggcgggaaga ttttccacag aaattgccga aatagaaaat gaaccgggat
      901 agatatgttt aatttgcaaa aatttttata atgcccattg aagccggaat ggcctccccc
      961 ccgttaaccg ccaaggattt tacaaccgac caggaggtcc gctggtgtcc gggttgtggc
     1021 gactattcca ttttagccca ggttcagaaa gtgatgccgc agctcggtat cccccgggag
     1081 aatatcgtga tcatttcagg gatcggctgc aacagccggt ttccctatta tatgaatact
     1141 tatggtatgc attccattca cggaagggca acggccatcg cttccggatt aaaggccagc
     1201 aggccggaac tgagtgtgtg ggttgttacc ggcgacggag attcgttaag tatcggagga
     1261 aatcatacca ttcacctgct tcgccggaat tttgacctga acatcctttt gtttaataac
     1321 cagatctacg ggcttaccaa gggtcagtat tcacccacat ccgaaactga taagctcacc
     1381 aagtctactc cattcggaag catcgaccat ccctttaacc ccctggcgct ggccatgggt
     1441 gcggatgcca gctttgtggc ccggagtatg gaccgggacc cgaagcacct gcagcaaatg
     1501 ctcatgcgca gccatcagca cagaggctcc tc
//
LOCUS       DASYCU010000620         6574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1147247, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000620 DASYCU010000000
VERSION     DASYCU010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147247"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VGM24_12430"
     CDS             complement(<1..566)
                     /locus_tag="VGM24_12430"
                     /inference="COORDINATES: protein motif:HMM:NF025680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4156235.1"
                     /translation="MKRFKFSKYIIISLASATMFYACSKNFLTVAPVGTLNPDILANQ
                     KGVQGLLVGAYSILDGQGGNNGGWGSAISNWVYGSVCADEAYKGSTSDDQGDIVPLET
                     WSANPANSYPSQKWDVNYDGIQRANQVLRVAKLATDISSTDLAVIQGEARFLRGFYHF
                     ELKKVFNNIPYVDESIADTSGQASVSNV"
     gene            complement(588..3803)
                     /locus_tag="VGM24_12435"
     CDS             complement(588..3803)
                     /locus_tag="VGM24_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY4156236.1"
                     /translation="MLSKTACRSRAYALVFLLFVSSGLFAQTVITGRVINNTDKQPVA
                     GATVQVRGSKLATQTGTDGTFSLSSNKPVTSLVITVVGFNPETVEVSGRTALGDIGLS
                     ISTTSLNDVVVTGYTSQKKKDITGSVSVVNVADMKSVPSGSTQSLLQGQASGVTVINS
                     GMPGGGSNVRIRGITSLGSSDPLYVIDGVQSINGMQDLNPSDIESVQILKDAGAAAIY
                     GVQGSNGVIVITTRKGRSGKTHISYDGYVGTQRPLPGNPFHLANTTEYATAIYDMETN
                     GGTADSNRTLQFGRAPGAGPVVPDYIFPEGAMNGDPGTDPSTYEVPGHQIMKANKAGT
                     DWFHEVFKPAFIQSHTISATGGGDRSSYYFSVNYLDQQGTLINTYLKRYSVRMNTVFN
                     VKDHIRVGENAYVFYKVNPGFTNQNEGNAISFVYRMPPIIPVYDIMGNFAGTQAPHLS
                     NSENPVAHQVRTKDNKGNDWQANGNVFAEVDFLKHFTARTSFGGTFDNYYYYYFNYTH
                     YEDAEGNTNANSFTEAAGYNSQWLWTNTLNYSNVWGKHDLKVLIGQEANKIYQRSFQA
                     GRTNYFLTDPNYWTLSTGDPGGVSNSGNPPTDKHLSSYFGRLDYNYDDKYLVSGVVRR
                     DASSVFAPDQQVGYFPSVTAGWRVSKETFFSDVSFINDLKIRGGWGKLGSISNINPTN
                     AYTLFASGVGYSWYPLDGAPNTSTQGFYPSQIGNAATTWEQDVITNIGLDGTILNNKL
                     EFSVEWYKKEIKGLLFRPDNSLGAMAGGATPAFINGGNIENKGIDANLTYHATVSSDF
                     HFDINANITSYHSKVVSLPGDKKYYDEGSAGSSRIGAFTRAQIGQPIGEFFGYKVIGL
                     FQSADDVSKSPTQDAAKAGRFKYADINHDGKISDSDRTFFGNPNPKFSYGFTLNASYK
                     NFDLSIFLYGTYGNDVINYVKYWTDFPNVFRGNVSKDAVYNSWTPSNPNAKVPILETN
                     GNFSNSGAFNSYYMEKGSYLRCKNLSIGYTVAPALLKNVGVDKLRIYIQAANLFTITK
                     YTGLDPELQGSDLGNNSNFGIDFGNYPANQKNYNVGVSLSF"
     gene            4193..4972
                     /locus_tag="VGM24_12440"
     CDS             4193..4972
                     /locus_tag="VGM24_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156237.1"
                     /translation="MKLKILLSLLSAALLTVSCNRYYYKPNAINTPLFTGGGQAHLNL
                     AGSVGNPDNGKNSEGNTYFFDIQGSVSPVNHLAFIANYSTYSYRVNNPDILSGHVDAS
                     AHLLEGGIGGYYASHGRKVQFVSDLFVGYGGGPVRSDVDMNASRFFIQPGIGMHSPWV
                     DAAFNLRFSVLKFSDFNSKGRDDDYLLDAKLIDGYGRRIDQRTYSFAEPGITLRAGYK
                     FAKVQLQMVLANEISNVAWRYNAARFTAGFYFSLEDALKMK"
     gene            complement(5076..6404)
                     /locus_tag="VGM24_12445"
     CDS             complement(5076..6404)
                     /locus_tag="VGM24_12445"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY4156238.1"
                     /translation="MKKLFLFLCLASGFAACNTKKDYEKVFKDPNLYCNAVHELNTVV
                     MGNNFSPIVASRNYLYSSVAGYEVIAGGYPEKYNSLGGQIHGLKPAPKPESGKQINFE
                     LASLLAFCKLGESVTFPEGSMKDYVDSLKNLAKDHGMPEEVLDNSIAYSDTVASGILE
                     WSKQDNYAQTRGAPEYSVNDSPGRWVPTPPAYAPAMEPHWSSIRYLVLDSLNQFMPPP
                     PYKFDITNKKSPYYLEVKKIEDKVDSLTPEEAKIADFWDDNPFKLNVSGHLMFGTKKF
                     SPGGHWMGIAGIAAIKSGADFSKTVSGFAETAIAMFDAFIQCWNFKYIYNTLRPETAI
                     NKYIDPNWKPHLQTPPFPEYTCGHCTISAASAEALSSVYGDQFPYTDSTELEFGIPKR
                     SFKSFRDAAEETQKSRFYGGIHYEYCTVISKNMGKDIGALVVKRLKMKKD"
BASE COUNT         1646 a         1541 c         1574 g         1813 t
ORIGIN      
        1 tccacattgg atacggaagc ctgtccggaa gtgtctgcaa tggattcatc cacatagggg
       61 atattattga agaccttttt cagttcaaaa tgataaaaac cacgcaggaa ccgggcttct
      121 ccctgtatta cggccagatc ggttgacgaa atatccgttg ccagtttcgc cacgcgaagc
      181 acctggttgg cgcgttgaat tccatcgtag ttaacatccc atttttggga aggataagaa
      241 ttggccggat tggcggacca ggtttccagc ggaacgatat ccccctggtc atctgaagta
      301 gatcccttgt acgcttcatc ggcgcataca cttccataaa cccaattgga aatggctgaa
      361 ccccatccgc cgttattacc gccctggccg tccaggatag aataggctcc gacgagcaga
      421 ccctgaactc ccttttggtt agccaggata tccggattga gggtgccgac cggcgcaacg
      481 gtcaggaagt tcttgctaca cgcgtaaaac atagttgcgg aagcaagtga tatgataatg
      541 tatttactga atttaaatcg tttcatatgg aaaaaattta agactgatta aaaggatagg
      601 cttacaccta cgttgtagtt tttctgattt gccggatagt taccgaagtc aattccaaaa
      661 ttggagttat tacccagatc ggatccctga agttccggat caagcccggt gtatttagtg
      721 atcgtaaaca gattggccgc ctgaatataa atcctcagtt tatcgacgcc cacatttttc
      781 aacaatgcag gagccacggt atatccgatt gaaaggttct tacaacgtaa ataggaccct
      841 ttttccatat aatacgagtt aaaggctccg ctgttgctga aattaccatt cgtttccagg
      901 ataggcactt tcgcattcgg attggaagga gtccagctgt tatatacagc atccttgctc
      961 acgttgcccc ggaatacatt ggggaagtcg gtccagtact ttacgtaatt gatgacatcg
     1021 ttcccgtacg ttccgtaaag gaaaatggaa aggtcgaagt tcttatagga agcattcagc
     1081 gtgaacccgt aagagaattt tggatttggg ttaccgaaga atgtccggtc gctgtcggaa
     1141 attttaccat cgtggttaat gtccgcatat ttaaatctgc cggcttttgc cgcatcctgg
     1201 gtgggtgact tggaaacgtc atctgcactc tggaataaac caatcacctt atatccgaaa
     1261 aactcgccga tcggctgtcc gatctgggcc ctggtaaagg ctccgatacg gcttgagcct
     1321 gcactacctt catcatagta tttcttgtct cccgggaggc ttacgacctt actatggtat
     1381 gaagtgatat tggcattgat atcgaagtgg aaatcgctgc ttaccgtcgc atgataggta
     1441 aggttcgcgt cgatcccttt gttttcaata ttaccaccgt ttataaaggc gggggttgcg
     1501 ccgccggcca tggcgccgag tgaattatcg ggacggaaga gcaggccctt gatttctttc
     1561 ttgtaccatt caaccgagaa ttccagttta ttgttcagga tggttccgtc aagtccgata
     1621 ttggtaatta cgtcctgctc ccaggtggtg gccgcattcc cgatctggct tggataaaat
     1681 ccctgggtgg aggtattggg agctccgtca agcggatacc aggaatatcc tactccggat
     1741 gcaaaaagcg tataggcatt agtggggttg atattgctga tagaaccgag cttgccccag
     1801 ccgccgcgga tcttcaggtc gtttatgaaa ctgacatcgc tgaagaaagt ttcctttgaa
     1861 acgcgccagc cggcggtaac agaaggaaag taacctacct gttggtcggg cgcaaatacc
     1921 gagctggcat cccggcggac tacgccgctc accaggtact tgtcatcata attataatct
     1981 aacctgccga aataggaaga aagatgtttg tcggtgggcg gatttcccga gttactcacg
     2041 cctccgggat ccccggtgct gagtgtccag tagttgggat ctgtaaggaa atagttcgtt
     2101 ctgcccgcct ggaaacttct ttgataaatc ttattggctt cctgtccgat cagcactttc
     2161 aaatcatgct tgccccagac attggaatag ttaagggtgt ttgtccataa ccactggcta
     2221 ttgtatccgg ccgcttcggt aaaggaattg gcattcgtat ttccttccgc gtcttcgtaa
     2281 tgggtatagt tgaagtaata gtagtaataa ttatcaaagg ttcctccgaa agaagtcctg
     2341 gccgtaaaat gtttgaggaa atccacttcc gcaaatacat ttccgttggc ctgccagtcg
     2401 ttccctttat tatccttggt ccgtacctgg tgcgccacgg ggttttccga attgctgaga
     2461 tgcggcgcct gcgtaccggc aaaattaccc atgatatcat ataccggaat aattggcggc
     2521 atgcggtaaa caaacgaaat ggcattgcct tcattctggt tggtaaaacc cgggttcact
     2581 ttgtagaaca cataggcatt ttccccaacc cggatatgat cctttacatt gaataccgta
     2641 ttcattcgca cggaatatct tttcaggtag gtattgatca gcgtaccctg ctgatcgaga
     2701 taattcacgg agaaataata agaggatctg tccccgcctc cggttgcact gatggtatgg
     2761 gactgaatga acgccggttt aaatacttca tggaaccaat cggtaccggc tttatttgcc
     2821 ttcatgatct gatggccggg aacctcatag gtggagggat ccgttccggg atctccattc
     2881 atggctcctt ccggaaatat ataatccgga accacagggc cggcaccggg tgcgcgtccg
     2941 aactgcagtg tccggtttga atcggctgtt ccgccgttcg tttccatatc ataaatggcc
     3001 gtcgcatatt ccgtggtatt cgccaggtgg aacggattcc cggggagagg ccgctgggtt
     3061 cccacataac cgtcgtaact gatatgggtc ttgccgcttc ttcctttgcg cgtggtgata
     3121 acgattactc cattggaacc ctgtactccg taaatggcgg ctgcgccggc atctttcagg
     3181 atctgaacgg attcgatatc actcggattc aagtcctgca ttccgttgat ggactgaacc
     3241 ccgtcgataa cataaagagg gtctgaactt ccgagggaag taataccccg gatccggacg
     3301 ttacttccac cacccggcat ccccgagttg atgaccgtta ccccggacgc ctgtccctgt
     3361 aacagggatt gcgtggaacc ggaaggaacc gatttcatat cggctacatt caccaccgaa
     3421 acggaaccgg tgatgtcctt tttcttctga gaggtgtatc ctgttaccac cacatcattc
     3481 agggaggtgg tggaaatgct caatccaata tctcccaaag ccgttctgcc tgaaacttca
     3541 accgtttcag gattaaatcc cacaacggta attaccaggg aggtaaccgg tttattggag
     3601 ctgagcgaaa atgttccgtc ggtacccgtc tgggtagcca atttggaacc tctcacctgg
     3661 acggtagcac ccgctacggg ttgtttatcc gtgttgttga taacacggcc tgtaattact
     3721 gtctgggcaa aaagtccgct ggaaacaaag aggagaaaaa ctaacgcata cgcgcgggag
     3781 cggcaagcag tttttgatag cataagcaat gtttaggttt aaataacact taaagctatg
     3841 ttaatttcga attgctgcca taaaaaattt caggtttttt gaaaaaacgg ggaaaatttc
     3901 aaaaatccat acatttttac ctcgttctat acaaaattga ccgtgattta atccttttta
     3961 aattttttta atttatccgg caggaattag attctctatc gctccccgcg agatcttttt
     4021 tgttcgctga ccggcagaat aaaatcctcc ttgcccgctg acccggatcc tctttgattc
     4081 cggggaattg cccgctgccg gccattcatg acctgaaaaa gacaatgttt ggcctgtttg
     4141 aacgttttaa atagattttg tccgggcatt gttcaattta aaatccagtc gtatgaaatt
     4201 aaaaattcta ctctctctac tatcggcagc actgctgacg gtcagttgca accggtatta
     4261 ttataagccc aatgcgatca atacgccttt gtttacggga ggtggtcagg cccacctgaa
     4321 tctggcaggc agcgtgggta atccggacaa tgggaaaaac agcgaaggaa atacctattt
     4381 tttcgatatc cagggatccg tttctccggt gaatcacctg gcatttattg cgaattacag
     4441 cacttatagt taccgggtga acaatccgga cattttaagt ggtcatgtgg atgcttcggc
     4501 ccatctgctg gaaggaggca tcgggggata ctacgcgagc catggcagaa aagttcaatt
     4561 cgtatccgat ctttttgtgg gctatggcgg tggtcccgtc cggtcagatg tggacatgaa
     4621 tgcctcgcgg ttctttattc aacctggtat cggcatgcat tccccctggg ttgatgccgc
     4681 ctttaacctt cgcttcagcg tgttgaaatt ttctgatttc aattcaaagg gaagggatga
     4741 tgattacctg ctggatgcca aactgattga cggttatgga agaagaattg accagcggac
     4801 ctatagtttt gccgaaccgg ggattaccct ccgggccgga tataaatttg ccaaggtcca
     4861 gctgcaaatg gtgctggcca atgaaatcag caatgtggcc tggcgttata atgccgcccg
     4921 ctttaccgca gggttttatt tcagtctcga ggatgccttg aaaatgaagt agggaaacag
     4981 atcccggaac aggctcctct ttcaggagac ggcagcctgc cggaaccgat aggcgctgaa
     5041 caggttctgg aatagtactc cctgtaaaat ttttattaat cctttttcat tttcaggcgc
     5101 tttaccacca gcgctccgat atcttttccc atattcttgc ttatcaccgt acaatactcg
     5161 tagtgtatgc ctccgtaaaa gcgtgacttt tgggtttcct cggccgcatc gcggaaagat
     5221 ttgaaagatc tttttggaat gccgaattcg agttcggtag aatccgtata gggaaactgg
     5281 tcgccataca cactggacaa cgcttctgcg gaggcagccg aaattgtaca atgaccgcag
     5341 gtatattccg ggaaaggagg cgtttgtaaa tggggtttcc agtttggatc aatgtattta
     5401 tttattgccg tttcgggtct aagggtattg tagatgtact taaaattcca gcactgaata
     5461 aatgcatcga acatagcgat ggcagtctct gcaaaaccgg atacggtttt ggaaaaatcg
     5521 gcgccggatt taatggcagc gataccggca attcccatcc agtgcccacc gggtgaaaac
     5581 ttcttggtgc caaacatcag gtggccggac acattcagtt taaatggatt gtcgtcccag
     5641 aaatcagcaa ttttcgcttc ttccggtgtg aggctgtcca ccttgtcctc aatctttttc
     5701 acctccaggt aataagggct ttttttgttg gttatatcga atttataagg cggcggcggc
     5761 ataaattgat tcaaactatc cagcaccagg tagcggatgg aactccagtg cggctccatg
     5821 gccggcgcat atgccggtgg cgtgggcacc cagcggccgg gagaatcatt taccgaatat
     5881 tctggtgctc cgcgggtttg cgcataatta tcctgtttgc tccattccag gataccggaa
     5941 gcgaccgtat ccgaataggc aatcgagtta tctaatactt cttccggcat cccatggtct
     6001 tttgccaggt tcttcaggct gtccacataa tctttcatgc tgccttccgg aaaagttact
     6061 gattccccta acttgcaaaa tgccagcagt gatgccagtt caaagtttat ctgcttgcca
     6121 gactccggtt tcggagccgg ttttagacca tgtatttgtc cgccaagtga attatacttt
     6181 tccggataac cgccggcaat cacttcatag cctgccacgg aagaatacag gtaattccgg
     6241 gaggcgacga tcgggctgaa attattgccc atcaccaccg tattcaattc gtgaacagcg
     6301 ttacagtaca ggttcggatc tttgaaaacc ttttcataat ccttttttgt gttgcatgcg
     6361 gcaaaacccg atgccaggca taagaacagg aatagcttct tcatcttacg atttgattta
     6421 aaaaagcgtg aacatacaaa ttaggggtca gtaaaaaaca cggcaatata aacggtattt
     6481 tttttcacta cgggaattaa ttttcccggt tgacctggtg tcaatagtaa ataagcgaag
     6541 aataagcgct ccgaagatat acaattattc acat
//
LOCUS       DASYCU010000621         6606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2166172, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000621 DASYCU010000000
VERSION     DASYCU010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166172"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..594)
                     /locus_tag="VGM24_12450"
     CDS             complement(118..594)
                     /locus_tag="VGM24_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEY4156239.1"
                     /translation="MIIHFDYDKKQVLRALRYHFISRPEIKIILIILNIFTILSAVLF
                     YFHKIQPLSFLSFSLLWFVLLITIWKLLPLSVYKRSQTFRDQFSMDFNEQDVVLQTER
                     GSHTWQWKEFSSFLESPHFFHLYFNSRSFFLVPKDAFKDLSVLQEARALLKEKLRK"
     gene            complement(1006..4374)
                     /locus_tag="VGM24_12455"
     CDS             complement(1006..4374)
                     /locus_tag="VGM24_12455"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEY4156240.1"
                     /translation="MVTHTQYASGLRFSRRFTREDIDVFDQFEYDYRSSVIRNPSGEI
                     VFEMTNVEVPRTWSQIATDILAQKYFRKAGVPQPDGSLGRETSVKQVAHRMANCWKVW
                     GIRYGYFASDADARIFYEELVYSILNQACVPNSPQWFNTGLYESYGITGKPQGHYYVD
                     QLDGQLKKSTSAYERPQPHACFILSVEDDLVNDGGIMDLWVREARIFKYGSGVGTNFS
                     NIRGEGEKLSGGGTSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDHPEIMDFI
                     NWKMEEEKKVGALIAAGYPSDYEGEAYKTVSGQNSNNSVRIPNSFFEKLENEEDWELK
                     ARSDGRIMKKIPAKEVWNQINYAAWRCADPGTQYDTTINEWHTCPNGGRIRASNPCSE
                     YMFLDNTACNLASVNLRRFYDETSNIIDVEGFEYTCRLWTAVLEISVLMAQFPSREVA
                     QLSYDYRTLGLGFANLGSMLMVMGIPYDSEEARGIAGAISAIMTGIAYKTSAEMAGAL
                     GAFPKFEENRESMLQVMRNHRLAAYDADEYEHLQTKPQGIKAKYCPDYLLTAATRAWD
                     DAVQLGEKNGYRNAQATVIAPTGTIGLLMDCDTTGVEPDFALVKFKKLSGGGYFKIIN
                     QSVPAALHNLGYGEREIDSIIKYAVGAGTFAGAPHINHQTLSEKGFFAEEIKKLDSAV
                     ASAFEIGFVFNVYNLGEECLKRLGFEPEQYYNFEWNLLEGLGFTDDQIAEANDYVCGT
                     MTLEGAPFLKDEHLPVFDCANKCGKIGERFIHAHGHIRMMSAVQPFISGAISKTINLP
                     NEATIEEIADAYLLSWKLGLKACALYRDGSKLSQPLSNKSDKKKKSDNTEEAKAKIPN
                     EPLVAMEPNIVDMSRLSVGELLEEVQKRVQSSPDTKLKRELATIVERRTLPAKRRGFT
                     QKAKINGQAIFLRTGEYADGTVGEIFIDMAKEGATMRSMLNCFAISISIGLQYGVPLE
                     EFVEKFVFTRFEPSGMVDHPNIKSATSIVDFIFRSLAYEYLGRADLVHVPDKPEVQNT
                     GDADWDHPIPEAEKKTPELSEVRLSKPVLSPAGQPMQKIVKNIKVETGLDAVNAAAKS
                     MQSDAPACNTCGHITIRSGTCYKCLNCGNSMGCS"
     gene            4577..5464
                     /locus_tag="VGM24_12460"
     CDS             4577..5464
                     /locus_tag="VGM24_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEY4156241.1"
                     /translation="MKKTRIVKKNFGRNGIINYRHPTRPGFQNRESFRTFVRPTASMK
                     DAIYSSLLHKKRNRRKSFAVLIDPDKTDPSNTARIIELSARARVDYLFVGGSLVISHQ
                     LDEVVQQIKASCHIPVILFPGSPSQISPYADGLLYLSLISGRNPELLIGQHVISAPIV
                     KKSGLEIISTGYMVIDGGAPTTVSYISNAAPIPADKNEIALCTAMAGEMLGMKLIYMD
                     AGSGARRPISEEMIQLVSSFIEAPLLIGGGIRDPEKVYLNAKAGADLVVVGSAIEKEA
                     SLLAEMTAAVHSVSVPERP"
     gene            5825..6547
                     /locus_tag="VGM24_12465"
     CDS             5825..6547
                     /locus_tag="VGM24_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4156242.1"
                     /translation="MEQEQKFKILVANASHQDFAQIICDEMESSAKARGTGIAKRSPD
                     YIKTKMREGKAVIAFTSDDIWAGFCYIETWSHGEYVANSGLIVAPHFRKSGLAKQIKN
                     KIFELSRKYYPDAKIFGLTTGLAVMKINSDLGYEPVTYSELTQDEAFWAGCKSCVNYE
                     ILMSKDRKNCMCTAMLYDPKEHAKPEETSRDFVRKSKLYERFMKFKQWGLLKHLIRKE
                     KVDMVLAEIGWLRPGKWKKQNG"
BASE COUNT         1664 a         1657 c         1613 g         1672 t
ORIGIN      
        1 gaagtttttt cttatgcact gtttcattcc gggagaccgt cgcttccgtt cgccggtttt
       61 cgcgtataaa atgtacgaaa ttcaaactgt tttccggccg atttttaaaa atacctgcta
      121 cttccttaat ttttccttta ataaggcccg ggcctcctga agcacgctca ggtctttaaa
      181 cgcgtctttc ggaacgagaa aaaaggaacg gctgttgaaa taaagatgga aaaaatgcgg
      241 gctttcgaga aagctgctga actccttcca ttgccaggta tgcgatccgc gctcggtttg
      301 caaaacgaca tcctgttcat tgaaatccat gctgaactga tcccggaaag tctgcgatct
      361 tttataaacg ctcagcggca gtaatttcca gatggtaatc aaaagcacaa accacagcaa
      421 tgaaaacgaa aggaaagaaa ggggctgaat tttatgaaag taaaaaagga cggcactcaa
      481 tatggtgaat atattcagga tgatgaggat aatcttaatc tccggcctgg aaataaaatg
      541 ataccgcaag gcacgaagca cctgcttttt gtcgtagtca aaatgaatga tcatccgctg
      601 aaattatggt acgcaatgta ctaaagaagc ggtatgattg atgatcaata agggggaaag
      661 aaaggattac ctcatattcc ggcagctgtt ataatggtgc gttgccgcct gatagggagt
      721 tacggagcgg ctgcaggaat acaacaggga aagaagtaat gcaagagcaa ggatcttttt
      781 catggggttc atttgaggta atagattaac ggacggatgc aatgaaaatt gctgaattaa
      841 taccaatttc cctgccagga tcagtgaaaa tcctgtccct tttccggaaa aaatgctgaa
      901 ttgggaacgg ggaggggatc cgggaaaagg ctaaaataaa gatcccgcat agatatgcgg
      961 gacctgtggt ttagtaaaac cgtccaaagt atcttcaaaa tgattttaac tgcagcccat
     1021 gctgtttccg cagttcaggc atttataaca ggtgccgctg cgtatggtga tatgaccgca
     1081 ggtattgcat gccggcgcat cgctctgcat actcttggct gccgcattca ctgcgtccag
     1141 gcctgtttcc acctttatat tcttaacaat tttctgcatc ggctggccgg caggcgacag
     1201 caccggcttg gacaacctga cttcacttaa ttccggcgtt tttttttcgg cctcgggaat
     1261 ggggtgatcc caatccgcat caccggtatt ctgcacttcg ggtttatcgg gcacatgcac
     1321 cagatcggcc cttcccaggt attcataagc cagggaccgg aaaataaaat caacaatgga
     1381 agtcgcactc ttaatattcg ggtgatccac catgccggat ggttcaaagc gcgtgaaaac
     1441 aaatttctcc acgaattctt ccaggggtac cccatattgc agcccgatgg aaatcgagat
     1501 ggcaaagcaa ttcagcatgc tccgcatggt agcgccttct ttggccatat caatgaagat
     1561 ctctcccacg gtgccatctg catattcacc ggtccggagg aaaatggcct ggccgttgat
     1621 ctttgctttt tgggtgaagc cccgccgttt tgccggcagc gttctgcgtt ccacgatagt
     1681 agccagttct ctttttaatt tggtatccgg ggaggactga actctttttt gcacttcttc
     1741 cagcagttcc ccaacggaaa gccggctcat gtccacgata ttcggttcca tggcgaccag
     1801 gggctcattg ggaatttttg ctttggcttc ttccgtattg tcggattttt tctttttgtc
     1861 ggatttattg ctgaggggtt gggaaagctt ggatccgtcg cggtaaagag cacaggcttt
     1921 caggcctaat ttccagctca gcaggtaagc gtcggctatc tcttctatcg tggcttcatt
     1981 gggaagattg atcgttttgg agatcgcgcc gctgataaag ggttgaacag cgctcatcat
     2041 ccggatatgc ccgtgcgcgt gaataaaccg ttcgccgatt tttccgcatt tgttggcgca
     2101 atcgaaaacc ggtaggtgtt catctttcag gaaaggggcg ccttccaggg tcatggtgcc
     2161 acagacgtaa tcattcgctt cggcgatctg gtcgtccgta aatccgagac cttccaggag
     2221 gttccattca aaattataat attgttccgg ctcgaatcct aaccgtttca gacactcttc
     2281 tcccaggttg tagacgttga aaacaaaacc gatttcaaag gcggaggcaa cggctgaatc
     2341 cagcttttta atttcttctg caaaaaatcc cttttcgctg agggtctggt gattgatatg
     2401 cggtgcgccg gcaaatgttc cggctcccac tgcgtattta atgatggaat cgatttccct
     2461 ttccccgtaa cccagattat ggagggcggc gggaacagac tggttgatga tcttgaaata
     2521 cccgccaccg gacaatttct taaatttcac cagcgcaaaa tcgggctcga caccggtggt
     2581 atcacagtcc atcaggaggc cgatggtgcc ggtaggcgcg atcacggtcg cctgcgcgtt
     2641 ccggtagccg tttttctctc cgagctgaac ggcgtcatcc caggccctgg tggctgccgt
     2701 tagcaggtaa tccgggcagt acttcgcctt aattccctgg ggtttggtct gcagatgttc
     2761 atattcatct gcatcataag ctgcaagccg gtgattgcgc atgacctgca gcatcgattc
     2821 gcggttctct tcaaactttg gaaatgcacc cagcgctccg gccatttcag cagaagtctt
     2881 gtaggcaatc ccggtcatga ttgcactgat ggcaccggca atacctctgg cctcttcact
     2941 atcatacgga atgcccatca ccattagcat ggaaccgagg ttggcaaagc caagcccgag
     3001 ggtccggtaa tcgtaggata actgagccac ctctctggat ggaaattgtg ccattaaaac
     3061 ggaaatttcg agcacggcgg tccataaacg acaggtgtac tcaaaccctt ccacatcaat
     3121 gatattgctg gtttcatcat agaacctgcg cagatttacg gaagccagat tacatgctgt
     3181 attgtccagg aacatgtatt cggagcaggg gttggatgcc cggatacggc caccattcgg
     3241 gcaggtatgc cactcgttga tggtagtatc gtattgggta cccgggtcag cacaacgcca
     3301 tgctgcgtaa ttaatctggt tccaaacctc ctttgcagga atctttttca tgatccttcc
     3361 gtcacttctt gctttcagct cccagtcttc ctcgttctcc agcttttcaa aaaatgagtt
     3421 ggggatgcgt acagaattat ttgagttttg tccactgacg gttttgtagg cctctccttc
     3481 gtaatcactc ggatatcccg ctgcgatcag tgcgcccact ttcttttcct cctccatctt
     3541 ccagttgatg aagtccatga tttccggatg atcgaggtcg aggcaaacca tttttgccgc
     3601 acggcgggtg gttccccccg atttaatggc tccggcagcg cggtcgccga tctttaaaaa
     3661 actcatcagg ccgctggatg tcccgccgcc gctgagtttt tctccttctc cgcggatatt
     3721 ggagaaattg gtgcctacgc cggatccgta tttgaaaatg cgtgcttccc ggacccagag
     3781 gtccataatg ccgccatcgt ttaccaggtc gtcctcgacg ctcaggataa agcaggcatg
     3841 aggctggggc cgttcgtaag ccgaagtgga ttttttcagc tggccgtcca gttgatccac
     3901 atagtaatgt ccctggggtt ttccggtgat cccgtaactt tcgtaaagac cggtattgaa
     3961 ccactgcggg ctgttgggca cacaggcctg attcaggatg gaataaacca attcctcgta
     4021 gaaaatccgg gcatcggcat cgctggcgaa atagccatac cggatgcccc ataccttcca
     4081 gcagttggcc atccggtgag ccacctgttt taccgatgtt tcccggccaa gggatccgtc
     4141 cggctggggc acgccggctt tgcggaaata tttctgggca agaatatccg tggcgatctg
     4201 gctccaggtc ctgggcactt ccacattggt catctcaaat acgatctccc ccgacggatt
     4261 ccggataacc gaactgcggt aatcatactc aaactgatca aacacatcaa tgtcttcccg
     4321 ggtgaagcgc cggctgaacc gcaggccgct cgcatattgc gtatgagtaa ccatgagaat
     4381 gaaggcttta actgtttttt ggaaaaaagg acgattgctc taaaaactac cggctgctta
     4441 ttaacgaatt gttaagtgct tacgaaagta ttttttgatt ttgaattttc ctcccctgaa
     4501 atattaacac atgtggaaaa gaatagttaa taattcatga agcacaagcg ggcattaccc
     4561 gggatgattt atgcacatga aaaagacccg gattgttaaa aagaattttg gccggaatgg
     4621 aataatcaat taccggcatc cgacccgccc gggttttcaa aaccgggaaa gttttcgcac
     4681 atttgtaaga ccaacagcca gcatgaagga cgcgatctac agctcattac tccacaaaaa
     4741 aagaaaccgg cggaagtcct tcgccgtact catcgatccc gataagacag atccgtcaaa
     4801 tacggcaaga attattgagc tctcggcccg ggcgcgggtc gactaccttt ttgtcggcgg
     4861 cagtctggtg atctcccacc agctggatga agtggttcag cagatcaaag caagctgtca
     4921 tatcccggtg atcctgtttc ccggatcccc ctcgcagatt tctccttatg ctgacggcct
     4981 cctttacctt tccctgatct ccggccgcaa cccggagctg ctgatcgggc agcacgtgat
     5041 ctccgccccg atcgtcaaaa aaagcggcct ggaaatcata tccaccggat atatggtgat
     5101 tgacggggga gcgcccacca ccgtttccta tatcagcaat gccgccccca ttccggcaga
     5161 caaaaatgag atcgcccttt gcacggccat ggcgggtgaa atgctgggaa tgaaactgat
     5221 ctatatggat gccggaagcg gcgccagacg gcccatttcc gaagaaatga tccagctggt
     5281 ctcttctttc atcgaagccc cgctgctcat cggcggaggc atccgggacc cggagaaagt
     5341 gtaccttaac gccaaagcgg gcgccgacct ggtggtcgtt ggcagcgcaa tcgaaaaaga
     5401 agcttccctg ctggccgaaa tgaccgccgc cgttcattcg gtttcagtgc cggaaaggcc
     5461 ctgatcctca agctcccgca aagaaaaaat gccccctgca attttagcaa aaattattgc
     5521 ccgcactttc taaaaaagct gtttctttgt atggttaaaa aaatcagtca tcccattttt
     5581 ctctgaaatg aaaaaaaact cacccatatc aatcctcacc ggaatggtat tgcacgcctt
     5641 caataagcgt gggttttttg tagcgcccgt atgcgttatt ttttctgttc gtcccattcc
     5701 cttttgggta cgacgttgat atattccatc catagaagtc cggcaggacc tatgccccgt
     5761 acccagtcgg gtcaatcaat caatggcaac gtcattggca tttcttatct catgtaatca
     5821 ttcaatggaa caggaacaaa agttcaagat acttgtcgca aatgcctctc accaggattt
     5881 tgcacagatc atttgcgatg aaatggaatc ttccgcaaag gcccgcggaa cagggatcgc
     5941 aaagagaagc cctgactata tcaagaccaa aatgcgggaa ggcaaagcgg tgatcgcttt
     6001 cacctccgac gatatctggg cgggcttctg ctatatcgaa acctggagcc acggcgaata
     6061 cgtggccaat tccgggctta tcgtggcacc gcatttccgg aaaagcgggc tggccaaaca
     6121 gatcaagaac aagatcttcg agctcagccg taaatactat cccgatgcca agatcttcgg
     6181 cctcaccacc ggcctggccg taatgaagat caattcggac ctgggctacg aaccggttac
     6241 ctattcggaa ctgacccagg acgaagcctt ctgggcagga tgtaaaagtt gcgtcaacta
     6301 tgagatcctg atgagcaagg atcgcaagaa ctgtatgtgt acagccatgc tctatgatcc
     6361 aaaagaacac gccaaacccg aagagacctc ccgggatttc gttcggaaga gcaagcttta
     6421 tgaacgcttt atgaagttca agcaatgggg tctcctgaaa cacctgatcc gcaaggagaa
     6481 agtggatatg gtgctggctg aaatcggatg gctgcggccg ggtaaatgga aaaaacaaaa
     6541 cgggtaaaaa caggctaaaa cggatcagat cgatgagtaa agaaaataag aaagtggttc
     6601 tggctt
//
LOCUS       DASYCU010000622         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1443245, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000622 DASYCU010000000
VERSION     DASYCU010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443245"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2636
                     /locus_tag="VGM24_12470"
     CDS             <1..2636
                     /locus_tag="VGM24_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY4156243.1"
                     /translation="PVSIDPELMALGDSKTPHEYNLAGIKITGSKYLDEALLISVSGL
                     TIGDKVMIPGGDNFSKAITKLWNQRLFSNVAIYYTKISGNDIYIEIHVTERPRLTNFI
                     FKGASKTESDDLKVKTGLIKGRVITENMKQTADENIKKYYADKGFQGVQVKIVETVDS
                     KTPNSENLTFNIDKGSKVRINQISFYGNEAIPSPKLKKQLKDTKEMTRFTLFPPKDSS
                     SYGVNKPVSFKEYIDGWGFLSFTRSKEFIDPYFRFKLFSSAKFNQKKYEADKDKVLDY
                     YNSFGYRDATIVSDTTYYNSKNQLNIGIKVDEGKKYYFGNVVWKGNTKYTDSVLNLIL
                     GIKKGDVYNLDILNKKLGKQVTQEGGGISDLYLDDGYLFFRIDPVETAVYNDTIDFEI
                     RIVEGPQATIKNVTIAGNEKTKEYVIRRELRTIPGEKFSRSDIIRSTREIVNLGFFNQ
                     EKVTPNIVPNQEDGTVDINWSVEEKSADQLELSAGWGGGIGVTGTVGVSFNNFSINNI
                     WKKSAWDPLPTGDGQKLSLRIQANGRAYQSYNLSFTEPWLGGKKRNSLTVSLYRSAFR
                     TGGYNYTTAKYDFSDTNSLKNFGVVLALGKQLKWPDDYFTLTYSLNYTRYDLTNYALF
                     SQDFRNGFSTNLSFKLALARYSIDQPIYPRSGSSFLASAEVTPPYSLFNKNYTDYQNF
                     KLPEFTKFRLNSEWYIPIGKAMGADKSRQFVLKAAVKYGFMGKYNSKMDYSPFERFQL
                     GDAGLTNNFGLLGYDIISQRGYPVYQNSDPRVNPDQQSASQFFVMFNKYTLELRYPFT
                     TNPSSTIYGEMFFEAANGWYDYKDYNPFRLRRDAGVGLRFFLPMFGLLGFDYAIGLDR
                     IQNGSLKGASRFTFMLGFEPE"
     gene            2659..3171
                     /locus_tag="VGM24_12475"
     CDS             2659..3171
                     /locus_tag="VGM24_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY4156244.1"
                     /translation="MKKIFLSFLFVSAVCLAASAQRIIVVDTKYILDKLPDYKTAQKQ
                     LDQTSAQWQKEIDDKQSTLDKMYKDYDGEKAMLTEELKKKREDELFNKEKEVRDLQRQ
                     RFGFEGDLFKKRQELIKPIQDKVYNAIQKIAVNRMYDIILDKSEGITVIFADPKLDKS
                     DDILKELGVK"
     gene            3236..3745
                     /locus_tag="VGM24_12480"
     CDS             3236..3745
                     /locus_tag="VGM24_12480"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY4156245.1"
                     /translation="MKKIVAILLVATGCLFAGSPLHAQTKMGYISLNELITAMPEYKK
                     ADTSMADFQSALNQNFDDMKKEFNEKDSLLSSKDTAKYTRAQIEIKRKQLGELYLRLQ
                     GYQQNASQQYQQKQQELMAPIQKRAQETVQQVAKENGYTYVFLKDALLVSPPAEDLLP
                     LVKKKLGLK"
BASE COUNT         1140 a          884 c          875 g          914 t
ORIGIN      
        1 atccggtttc cattgatccc gaactgatgg ccctcggcga ttccaaaaca cctcatgaat
       61 ataacctggc cggcattaag attaccggca gtaaatacct cgatgaagcc ctgttgattt
      121 ccgtatccgg attgactatt ggcgacaaag tgatgatccc cggcggcgat aatttcagca
      181 aggccattac caaactctgg aaccagcgtc tcttctccaa cgttgcgatt tattatacta
      241 aaatttccgg caacgacatc tatatcgaga tccatgtaac ggaaaggccc cggctgacca
      301 attttatttt caaaggagca agtaaaaccg aatcggatga tctgaaagta aaaaccggtt
      361 tgatcaaagg ccgcgtgatt acggaaaaca tgaagcagac cgctgatgaa aatattaaga
      421 aatactacgc tgacaaaggg tttcaggggg ttcaggtaaa aattgttgaa acggtcgatt
      481 ccaaaacgcc caattcggaa aaccttacgt tcaatatcga caagggatcc aaggtcagga
      541 taaaccagat cagtttttat ggcaatgaag cgatcccttc ccccaaattg aaaaagcagc
      601 taaaggatac caaggaaatg acccgcttca cgcttttccc gccgaaggat tccagttcct
      661 atggggtgaa caagcccgtt tcttttaaag aatatatcga cggctggggc tttctttcct
      721 ttacccggtc gaaggaattt attgatccgt atttccgctt taaattattc agttccgcca
      781 agttcaatca aaaaaaatac gaagcggaca aggacaaggt cctggattat tataattcct
      841 tcggataccg cgatgcaacc atcgtttccg ataccactta ctataacagc aaaaaccagt
      901 tgaatatcgg catcaaggtg gatgaaggaa aaaaatatta tttcgggaat gtagtctgga
      961 aggggaatac aaaatatacc gactccgtgc tcaaccttat cctcggcatt aagaaaggag
     1021 atgtatataa cctggatatc ctcaataaaa aactggggaa acaggttacc caagaaggcg
     1081 gcgggatcag cgacctctac ctggatgacg gatatttgtt tttccgtata gatccggtgg
     1141 agaccgccgt ttacaatgat acgatcgatt tcgagatccg gatcgtggaa ggcccccagg
     1201 caaccattaa aaacgtgacc atcgccggca acgaaaaaac caaggaatat gttatccgga
     1261 gggagctgcg cacgattccc ggagaaaagt tcagccgttc cgatatcatc cgttcaaccc
     1321 gcgaaatcgt aaaccttgga ttctttaacc aggaaaaagt aacgccgaat atcgttccga
     1381 accaggaaga cggaaccgtg gatatcaact ggtccgtgga ggagaaatct gccgatcagc
     1441 tcgaactctc tgcaggctgg ggcggcggga tcggggtcac cggtaccgtt ggggtatcct
     1501 tcaataactt ttcgatcaac aatatctgga agaaatcggc ctgggatccc ttacccaccg
     1561 gagacggaca aaaactgagc ctccgtatcc aggccaacgg aagggcctat cagtcctata
     1621 atctgtcctt tactgaaccc tggctgggcg gtaaaaaacg gaactccctg acggtgagct
     1681 tataccggag cgccttccgg acgggcggtt ataattatac cacggcaaaa tatgatttca
     1741 gcgataccaa ttcattgaaa aacttcgggg tggttcttgc cctgggcaaa cagttaaaat
     1801 ggccggatga ttattttacc ctcacgtatt ccctgaatta tacccgctac gacctgacga
     1861 attatgccct gttttcacag gatttcagaa atggattttc aacgaacctg agtttcaagc
     1921 ttgccctggc ccgatattcc atagatcagc ccatctatcc cagaagcgga tccagtttcc
     1981 tggccagcgc cgaggtcacg ccgccctatt ccctgttcaa taagaattac acggattatc
     2041 agaattttaa attgcctgaa ttcaccaaat tccggctcaa cagcgaatgg tatattccca
     2101 tcgggaaagc catgggcgcc gacaagagcc gtcagtttgt gctgaaagct gcggtgaagt
     2161 acggatttat gggcaagtat aacagcaaaa tggactattc tccctttgaa agatttcagt
     2221 tgggagatgc cggtttaacc aataacttcg gtttgctggg atatgatatc atttcccaaa
     2281 gggggtaccc ggtttaccag aattcagatc cccgggtgaa tccggaccag cagtcggctt
     2341 cccagttctt tgtcatgttt aacaaataca cgctcgaact gcggtatccg ttcaccacca
     2401 atcccagcag caccatttat ggggaaatgt tttttgaagc cgccaacggc tggtacgact
     2461 ataaggatta taatcctttc cgtctgagac gtgacgccgg tgtagggctg cggttcttcc
     2521 tgcccatgtt cggattgctc ggatttgact atgccatcgg gctggaccgt attcagaatg
     2581 gttctctgaa aggggcatcc cgattcacct ttatgcttgg atttgaacct gagtaaagca
     2641 ggcttaaaaa caaacgatat gaaaaaaata ttcctgtcct ttctctttgt ttccgctgtc
     2701 tgcctcgcgg catctgccca gcgtattatc gtggtcgata ccaaatacat cctggataaa
     2761 ttgccggact acaaaacggc tcaaaagcag ttggatcaga ccagtgccca gtggcagaag
     2821 gaaatagacg ataaacagtc gaccctggat aaaatgtata aggattatga tggggaaaag
     2881 gcgatgctga cggaagaact gaagaaaaaa agggaggatg aactcttcaa caaggaaaaa
     2941 gaagtcaggg acctgcagcg ccagcgcttt ggctttgagg gggacctgtt taaaaagcgc
     3001 caggagctca tcaaacccat acaggataag gtttataatg ccattcagaa gatagccgtt
     3061 aaccggatgt acgacattat tttggataaa agtgaaggaa ttaccgttat ctttgccgac
     3121 cccaaactgg acaagagtga cgacatttta aaagaactgg gggttaaata gcctgtatcc
     3181 aactattaca cacaatttca actattaaaa aacaaaaaga aaactgaacg caattatgaa
     3241 aaaaattgtt gccatcctat tagtagcaac cggttgcctg tttgcaggat ccccgttaca
     3301 tgcgcaaacc aagatgggtt atatcagcct gaatgaattg atcacggcca tgccggaata
     3361 caagaaagcg gatacctcca tggccgattt tcagagcgcc ctgaatcaga atttcgatga
     3421 tatgaagaag gaattcaatg aaaaggacag cctgctttct tccaaggaca ccgcaaagta
     3481 cacccgggcc cagatcgaga tcaaaagaaa acaactggga gaactttacc tgaggttaca
     3541 gggataccag cagaatgcca gccagcagta tcagcaaaaa cagcaggaac tgatggcgcc
     3601 cattcagaaa agagcccagg aaacggttca gcaggttgca aaagaaaatg gctacaccta
     3661 tgtgttcctg aaagatgccc tgctggtttc gccgcctgcc gaagatttgc ttcccctggt
     3721 gaaaaagaag ctcggactga aatgatcccg gtataaaaat attacaggcc atcctgtctc
     3781 cggcaggatg gtttttttat tttttacttt tta
//
LOCUS       DASYCU010000623         7684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5060500, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000623 DASYCU010000000
VERSION     DASYCU010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5060500"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2551
                     /locus_tag="VGM24_12485"
     CDS             <1..2551
                     /locus_tag="VGM24_12485"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY4156246.1"
                     /translation="GLQEFKNLVGIKFQLYNSLFTSLPFHRIEKTGIFLSLLLTYCEE
                     GYHKKLSPLQIINDFFSKHSPDGTDREKADTLFRFVQYVERQVVLFDALEDAAFTGVN
                     DMGGPGTLKQLLSEVVQYNKEEELKEKLKDYAVRLVLTAHPTQFYPGPVLGIINDLSR
                     AVIENNAAQINVFMQQLGKTPFLKKHKPTPFDEAISLIWYLENVFYHAAGKILSSLKN
                     QFPEAVSEQNPVIQMGFWPGGDRDGNPFVNTATTLKVAESLRGTILRCYYLDVRRLKR
                     RLTFKEVDVIIADLEEKLYQELFKPDPKSPLNVGYMLTAMARIREILIYQNNGLFLHL
                     AENFISKIRIFGFHFATLDLRQDSSVHTAVLKRVNEREKLFDENYESLPEQEKIKALS
                     ELKPVAETSFPDEMVNDTLESIRAVKKIQEKNGPLGCNRYIISQCNSALNMMEVYGLF
                     LLGGWKKESLPVDIIPLFETVEDLRSAAEIMASLYSNADYRSHLERRKNTQTIMVGFS
                     DGTKDGGYLMANWGIYKAKEELTRISDQYGVRVVFFDGRGGPPARGGGKTHRFYASLG
                     KNIANKEIQLTVQGQTVSSNFGTVDSAQFNIEQLVHAGIANDLFSARKVTLNEEEESL
                     MEEMAGISYHSYTALRDHPDFMEYLNEVSPLQFYGETNIGSRPSKRKNSSKLELKDLR
                     AIPFVGSWSQLKQNVTGYYGVGTALQEMEKIGKWNAVKQLYAHSPYFKTLLDNSEMAM
                     KKCFFPLTESFSRHPRFGKIWTLIYNEFELTKKFLVKLSGNPDLMSDYPIEQLSIQMR
                     ERIVLPLSTIQQYAIMKYREAEKSGSSMKDIYEKLIIRCSFGVINAARNSA"
     gene            2681..3565
                     /locus_tag="VGM24_12490"
     CDS             2681..3565
                     /locus_tag="VGM24_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156247.1"
                     /translation="MIKRIFWNLYFLTFLNGFLLATLFYFKMEADYETELFQAIRTDV
                     NSKIDRGNSQDSVVVKVMHACHDLLTNREAVFEGKSLGGVKADLLHPTTIDLMTANGA
                     CGSFSIVLARLFEGYNLPVRIAQMKAKGIYAAHNIVEVKTNHGWAVMDPLFDVYFVNP
                     GHHLASFAEVQADWEHYRQQLPKGYDSNYRYADVRYSNWTKIPVIFPAVKKILDLTIG
                     KPAADTISIRTYFLRMYDICFGITLALFILVFFMTMMKLIKAKVFPQKNIPFTFSNIF
                     KYLRLRLVDRRIAGQGQS"
     gene            3666..4640
                     /locus_tag="VGM24_12495"
     CDS             3666..4640
                     /locus_tag="VGM24_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY4156248.1"
                     /translation="MLKVGVFGTGHLGKFHLNNWKEIREAEIVGFYDPDPVTAAEVSE
                     KFRIKSFDSPAELMDLCDAADIIAPTNFHFELCEQAIRRGKHVFVEKPLANNMEEARE
                     LLKLVKESNIKLQVGHVERFNPAFLALGDTPLHPMFIEVHRLAEFNPRGTEVSVILDL
                     MIHDIDIILHIVDSEVKNIYSSGVAVMTDTPDIANVRIEFNNGCVANLTSSRISMKKM
                     RKMRIFQKDAYIGVDFLAKKTEIISLNQADDADGFTFDIETRNGTKSISVSNPPVPEV
                     NAIKMELEKFTESIIKNKATMVSEMDGYRAMDIAHQILQKIKYNSVNS"
     gene            4649..6061
                     /locus_tag="VGM24_12500"
     CDS             4649..6061
                     /locus_tag="VGM24_12500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY4156249.1"
                     /translation="MGPGKKIHIIWYGLSDFLCAVISWIILYFTRRILLSEPVYLNGA
                     IYLNNRFWLGLSLIPAGWLIFYGLTGAYHSLYKKSRLNEFTSTGVCSLIGCTVLFFAI
                     VINDPQTDYRYYYKAYFIFLLAHFLITWLGRWVLLTITKRQLHEGKIIFNSLLLGSGP
                     IALKTYEESLVGLKGSGYHYSGYINVGPSDINSIPIPSLGRPADLEKIIDRENIRLVV
                     IAMEKTENDYAESIIQRLSEKDVEIKITTGIMEILSGSVKTSNIFSAVLGNIHTGLMP
                     EWQQHIKRLLDVLISGIALIVFSPILIYSAIRVRIGSAGPIIYSQERIGYKGRKFLIH
                     KFRSMEKDAEKNGPSLSSEKDGRITKWGRTMRKWRIDELPQLLNVIRGEMSLVGPRPE
                     RKYYIDLIMEKTPYFKYLLKVKPGVTSWGMVQFGYAENIEEMIERMKYDLIYIENISL
                     GLDLKIMLFTLLTIFKGKGR"
     gene            6083..>7684
                     /locus_tag="VGM24_12505"
     CDS             6083..>7684
                     /locus_tag="VGM24_12505"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY4156250.1"
                     /translation="MKKFILFPALLLSLLASGQEAWRQGLKYRLQVKLDDKQKTLEGS
                     MDLRYTNHSPDTLHFIWFHVWPNAYRNDRTGYSEQLLENGKTGFYFSDKESKGYINRL
                     EFRVNGIMADIQDHPEYIDVIKLILPAALAPGDSIRVDASFHEKLPYNFSGYGFRGHN
                     FQVSNWYPEPAVYDREGWHTMPFLEQGGAYHEAADYEVGIDVPDTYTLAAGNSADTVL
                     RQDNSDIKTYRFSLRKANSFAWIAGQRLTEKKDTVQLETGKQIRLNMYYFRRDSSLMQ
                     DQLGLSKSYIRQLSSLVSEYPYSSLTLIETLQPQEQSFSGMILMDVPGLYKNPERILR
                     RALAAQWFQAICLSDERLNPWMSGGFIRYYDQRLQPNPTPSRYQKHRVLDDRIWLRVA
                     EREKTAQPIRTPAEKMSPENYSLIAGTKSALWIKSLEDSLGEIKFDRGMRSYFEKWKF
                     GHPGPEDYKAAMEESGGIHLDSEFEELNTTRSLFPAGPKKMLKPAYGFSRINQDKYNY
                     INIAPAFGYNRYDDFMIGAIVHNYTL"
BASE COUNT         2075 a         1890 c         1885 g         1834 t
ORIGIN      
        1 cggattgcag gagttcaaga acctcgttgg aattaaattc cagctctaca atagtttgtt
       61 cacttcgctg ccctttcacc gcattgaaaa gaccgggatt tttctatccc tgctgcttac
      121 gtattgcgaa gagggatacc ataaaaagct cagtccgctg cagatcataa atgatttttt
      181 cagcaagcat tccccggatg gaacggaccg ggagaaagcc gatacccttt tccgttttgt
      241 ccaatatgtg gaaagacagg tggtgctttt tgacgcgctg gaagatgcag cctttaccgg
      301 agtgaacgat atggggggac ccggtacgct caaacagctt ttgtcggaag tggtgcaata
      361 caacaaggaa gaggagttga aggagaagct gaaggactat gccgtgcggt tggttttaac
      421 cgctcatccc acccagttct atccgggacc ggtgctcggc atcatcaacg atctttcgag
      481 ggctgtaatc gaaaacaatg cggctcagat caatgtcttc atgcagcaat tgggcaaaac
      541 ccccttcctc aaaaaacaca aacccacgcc tttcgacgag gccatcagcc tgatctggta
      601 tctggagaat gtcttttatc atgccgcggg caagatcttg tcttccttaa aaaaccagtt
      661 ccccgaagcc gtctccgagc agaatccggt cattcaaatg ggattctggc ctggcgggga
      721 ccgggacgga aatccctttg taaataccgc gaccaccctc aaggtcgccg aatccctccg
      781 cggcacgatc ctgagatgtt attacctgga tgtgcgaagg ctgaaacgcc ggctcacctt
      841 caaggaagtg gatgtgatca tcgcagacct cgaggaaaaa ctataccagg agctcttcaa
      901 acccgatccg aaatcaccct tgaacgtcgg gtacatgctg acggccatgg cccggatcag
      961 ggagatcctg atctaccaga acaacggcct tttccttcac ctggcagaaa acttcatcag
     1021 taagatccgg attttcggat ttcactttgc aacgcttgat ctgcgccagg acagttccgt
     1081 gcataccgca gtattaaaac gggtgaacga acgggaaaaa ctatttgacg aaaactatga
     1141 atcccttccg gaacaggaaa agatcaaggc actgagtgag ctgaaacccg ttgcggaaac
     1201 ttcattcccg gacgaaatgg tgaacgatac cctggagagc atccgggcag tcaaaaaaat
     1261 ccaggaaaaa aacggcccgc tgggctgtaa ccggtatatc atcagccagt gcaacagcgc
     1321 actgaatatg atggaggtgt acggattgtt cctgctgggc ggctggaaaa aagaatccct
     1381 gcccgtcgat atcattcccc tctttgaaac cgtggaggat ctgagaagcg ctgcggaaat
     1441 catggcctcc ctgtacagca atgcggatta ccggtcacac ctggaacgga gaaaaaatac
     1501 gcaaacgatt atggtcgggt tttcagacgg caccaaggat ggcggctacc tgatggcaaa
     1561 ctggggcatt tacaaggcaa aggaagagct caccaggatt tccgaccagt acggggtcag
     1621 ggtggtattt tttgacggca ggggcggtcc gcctgcccgc ggtggcggaa aaacacaccg
     1681 cttttacgcc tccctgggga agaatatcgc gaacaaggaa atacaactca ccgttcaggg
     1741 gcagaccgtg agctccaatt tcggaacggt ggattctgcc cagttcaata tcgagcagct
     1801 ggtacatgcc ggtattgcca atgacctgtt ttccgcccgg aaagtaaccc tgaatgaaga
     1861 agaggagtcc ctgatggaag aaatggccgg gatcagctat cattcctata cagcccttcg
     1921 ggatcacccc gacttcatgg aatatctcaa tgaagtgagt ccgcttcaat tttatggcga
     1981 aaccaatatc ggcagccgcc ccagtaaaag gaaaaattcc agtaagctgg aacttaagga
     2041 cctgcgcgcc attccttttg tcggttcctg gagccagctc aagcaaaacg tgaccggata
     2101 ttacggggtc ggaacagccc ttcaggagat ggaaaaaata ggtaaatgga atgcggtcaa
     2161 acagttatat gctcattccc cctatttcaa aaccctgctg gacaacagcg aaatggccat
     2221 gaaaaaatgt tttttcccat tgactgaaag tttttccagg catccccggt tcggcaaaat
     2281 ctggaccctt atttacaatg aattcgaact caccaaaaag ttcctggtca aactgtccgg
     2341 aaacccggat ctgatgtccg attatccgat cgagcagctc tcgatccaga tgagggaaag
     2401 aatcgtgctg ccgctcagca ccatacagca atacgccatc atgaaatacc gggaagctga
     2461 aaaatcgggt tcttccatga aagatatata tgagaagctc attatccgtt gctctttcgg
     2521 agtaataaat gcggccagaa attctgcctg accaccggta gccgcccgaa cttcggccgt
     2581 actggtatga aaattgccgt tttatcacag catttcgcgt gaatacccgt gttttggcta
     2641 actttgaaca ggccgattaa tcagtaagat tcgcatcaga atgataaaac gtattttctg
     2701 gaacctgtat tttcttactt tcctgaatgg ctttttactg gctacacttt tctatttcaa
     2761 gatggaagcg gattacgaaa cggaattgtt ccaggccatc cggacggacg tgaacagcaa
     2821 gatcgaccgc ggcaacagcc aggattccgt cgtggttaag gtcatgcatg cctgccatga
     2881 cctgcttacg aaccgggagg ccgtttttga aggcaagtcc ctgggcggtg tcaaggcgga
     2941 tctcctgcat ccgaccacca tcgatcttat gacggccaac ggggcctgcg gcagcttttc
     3001 cattgtactt gcccgtctct ttgaaggata taatcttccg gtccgcatag cccagatgaa
     3061 ggcaaaggga atctatgcag cgcataatat tgtggaagta aaaaccaacc acggatgggc
     3121 cgtgatggac ccgctttttg atgtctattt tgtaaaccca ggtcaccacc tggccagctt
     3181 cgccgaggtg caggccgatt gggaacacta ccgccagcaa ttgccgaaag ggtatgacag
     3241 caattaccgg tatgctgatg tccgttattc caactggacg aagatcccgg tcatctttcc
     3301 ggccgtaaag aaaattctgg accttaccat cggaaagccg gcagcggata ccatttcaat
     3361 cagaacctat tttctgagaa tgtatgatat ctgtttcggg atcacacttg cccttttcat
     3421 cctggtattc tttatgacga tgatgaaact gataaaggca aaagtattcc cccaaaaaaa
     3481 tattcccttt actttttcca atatttttaa atacctccgc ctccggcttg ttgaccgccg
     3541 gatagccggg caggggcagt cctgaccgca gatttcccac atttccacat ttgcagcggt
     3601 ttaagccaga tcgttcctgc ccacgtcttg cacattattt atatttgact acttaaattc
     3661 tttggatgct aaaagttggc gtattcggaa ccggacatct gggaaaattt catttgaata
     3721 actggaagga gatccgggaa gcggaaatag ttggttttta tgacccggac cccgtgaccg
     3781 cggcggaggt ttcagaaaaa ttccgcatta aatcctttga cagcccggcg gagctgatgg
     3841 atttatgcga tgccgcagat attatagctc ccaccaattt ccattttgaa ctttgtgaac
     3901 aggccatccg gaggggtaaa cacgtgtttg ttgaaaaacc cctggccaac aacatggaag
     3961 aggcgcggga attgttaaag ctggtaaaag aatccaatat taaacttcag gtcgggcacg
     4021 tggaacggtt caatccggca tttcttgccc tcggcgacac gccgctccac cccatgttta
     4081 ttgaagtcca ccgcctggcc gaattcaacc cgcggggtac agaagtgagt gtgatcctgg
     4141 acctgatgat ccatgatatc gatatcatcc tccatattgt ggatagtgaa gtgaaaaata
     4201 tatattccag cggagtggcg gtaatgaccg atacgccgga tattgccaat gtgcgcattg
     4261 aatttaataa tggctgcgta gccaatctca cgtccagccg gatctccatg aagaaaatgc
     4321 gaaagatgcg catcttccag aaggatgcct atatcggcgt ggattttctg gcaaaaaaaa
     4381 cggagatcat cagtttgaat caggcggatg atgccgacgg tttcaccttc gatattgaaa
     4441 cccgcaacgg gacaaaaagc atttcagtat ccaacccgcc cgtgccggag gtcaatgcca
     4501 tcaaaatgga actggaaaaa tttaccgaat ccataataaa gaacaaggcg acgatggttt
     4561 cggagatgga tggctaccgg gccatggata tcgcccacca gatccttcaa aaaataaaat
     4621 acaactccgt caatagctga caaaatgcat gggaccggga aaaaaaatcc atatcatctg
     4681 gtacgggctg agcgattttt tatgcgccgt aatttcatgg atcatcctgt attttacccg
     4741 ccggatattg ctttccgaac ccgtttatct gaatggcgcc atttatctca ataaccggtt
     4801 ctggctggga ctgagcctga ttcccgcggg gtggctgatc ttttacgggc ttaccggcgc
     4861 ttaccattcg ctctataaaa aatcgagact gaatgagttt accagcacgg gggtttgcag
     4921 cctgatcggc tgcaccgtcc tgttttttgc catcgtgatc aacgatccgc aaaccgatta
     4981 ccggtattat tacaaagcgt attttatctt tctgctggcc catttcctga tcacctggtt
     5041 aggccgctgg gtcctgctga ccattacgaa aaggcagctt catgaaggga agatcatttt
     5101 caacagcctc cttctgggtt cggggccgat tgcattgaaa acctacgagg aaagccttgt
     5161 cggacttaaa ggaagcggct atcattattc cggatatatc aatgtcggtc cttccgatat
     5221 aaacagcatt cccattccct ctctcggccg gccggcagac ctggaaaaga tcattgaccg
     5281 ggaaaatatc cgcctggtgg tgatcgccat ggagaaaacc gagaacgatt acgctgaaag
     5341 tatcattcag cggttgagtg aaaaagatgt ggagatcaaa atcacgaccg gcattatgga
     5401 gatcctttcg ggttccgtaa agaccagcaa tattttcagc gcagtgctgg gcaatatcca
     5461 tacggggttg atgcctgaat ggcaacagca tatcaagcgt ctcctggatg tgctgatctc
     5521 aggtattgcg ctgatcgttt tttcccccat cctcatatat tcagcgatcc gggtaagaat
     5581 cggatcagcc gggcccatta tctattcaca ggaaaggatc ggctacaaag gaagaaaatt
     5641 tcttatccat aaattccgtt ccatggagaa agacgcggaa aagaacggcc cttccctttc
     5701 ttcggaaaag gacggccgca tcacgaaatg gggccggacc atgagaaagt ggcgcattga
     5761 cgagcttccc cagttattaa acgtgatccg gggagaaatg agcctggtgg ggccccggcc
     5821 ggaacgcaag tattacattg acctcatcat ggaaaaaacg ccttatttca aatacctgtt
     5881 gaaagtaaag cccggagtga cttcctgggg aatggtacaa ttcggttatg cagaaaacat
     5941 tgaagaaatg atcgaaagga tgaaatacga tctgatttat atcgagaata tttcgctggg
     6001 gctcgacttg aaaatcatgt tgtttacctt gctaactatt tttaaaggca aaggccgata
     6061 acccgttcaa accggcttct cattgaaaaa atttattctc tttcccgccc tgctcttatc
     6121 gctgctggcg tccggtcagg aggcctggcg gcagggcctg aaataccggc tgcaggtgaa
     6181 actggatgat aaacaaaaaa cgctggaagg cagcatggat ctccggtata cgaaccactc
     6241 cccggacacg cttcatttca tctggtttca tgtttggccg aatgcatacc ggaacgacag
     6301 aacgggctat agcgagcaac tgcttgaaaa tggaaagacc ggtttttatt tctccgataa
     6361 ggaaagcaaa ggatatatca accgcctgga attccgggtc aatgggatca tggccgatat
     6421 tcaggatcat ccggaatata tcgatgtgat caaattgata ttaccggccg cgttggctcc
     6481 gggcgacagc atccgggtgg atgcttcctt tcatgagaaa ctgccctata atttttcagg
     6541 ttacggattc cgggggcata acttccaggt gagtaactgg tatcctgagc ccgcagtata
     6601 tgaccgcgaa ggctggcata caatgccttt cctggaacag ggcggtgctt accacgaggc
     6661 ggccgattat gaggtcggga tcgacgttcc ggatacctat accctggccg ccggcaattc
     6721 ggctgatacc gttttacgcc aggataacag cgatatcaag acctaccgct tttctttacg
     6781 aaaggccaat tcttttgcct ggatagccgg ccagcggctg acggaaaaaa aggataccgt
     6841 tcaactggaa acagggaaac agatcaggct gaatatgtac tatttccgaa gggacagcag
     6901 tctgatgcag gaccagctcg gcctttcgaa atcgtatatc cggcaacttt cctccctggt
     6961 ttccgaatat ccctattcca gcctgacgct gatcgaaacc ctacaaccac aggagcaaag
     7021 tttttccggc atgatcctga tggatgtgcc cggtctgtat aaaaacccgg aaagaatact
     7081 acgacgggcg ctggcggcgc aatggtttca ggccatctgc ctttccgatg aaagattaaa
     7141 tccgtggatg tccggcggat tcatccggta ttatgaccag cggctgcaac caaaccctac
     7201 cccgagccgt taccagaagc accgcgtact ggatgaccgg atctggctgc gtgttgccga
     7261 aagggaaaaa accgcgcaac ccatccgcac gcctgccgaa aaaatgagcc cggagaatta
     7321 ttcactgatt gccggcacca agtctgccct gtggataaaa tccctggaag attcactggg
     7381 agaaataaaa ttcgaccggg gcatgcggtc ctattttgaa aaatggaaat tcggtcatcc
     7441 cggcccggag gattataagg ccgctatgga agaatcggga gggatccatc tggattccga
     7501 atttgaagaa ctgaatacca cccggagcct ttttccggca gggccaaaaa aaatgctgaa
     7561 gccggcctat ggattttcca ggataaacca ggacaaatac aattacatta atatagcccc
     7621 cgctttcggt tataaccggt acgacgattt catgatcggt gcgatcgttc ataattacac
     7681 gctg
//
LOCUS       DASYCU010000624         6370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1007952, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000624 DASYCU010000000
VERSION     DASYCU010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007952"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1176
                     /locus_tag="VGM24_12510"
     CDS             160..1176
                     /locus_tag="VGM24_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEY4156251.1"
                     /translation="MIDLRSDTVTRPGPGMLQAMMQAAVGDDVFREDPTVIELEQTVA
                     GLFEMEAALYCASGTMSNQVAIKCHTHPGDEVICEQEAHVYIYEGGGIAFNSGSQVKA
                     IAGDRGRIQAGQIPGSLNPDDVHKARTSLVCLENTSNRGGGSCYDIGEIKKIRAVCDE
                     KKLNLHLDGARLFNALVARNEKPPEYGRLFHSISVCFNKGLGCPVGSVLLGTAGFIHQ
                     ARRIRKVFGGGLRQAGYLAATALYALEHQVERLAEDHAHAKQIAAALESMPVVQSILP
                     VETNLIIFELVPGEDPKLFAARMKAEGILLLPVSSTRLRMVTHLDISSEMTDQVCRAI
                     RNLS"
     gene            complement(1219..1437)
                     /locus_tag="VGM24_12515"
     CDS             complement(1219..1437)
                     /locus_tag="VGM24_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156252.1"
                     /translation="MSQAFVKENEEQWLHEVPPSVTALIYYLTRENNGIRVYEKKMVR
                     DTESGREVHVMSNGLSYSKDEKSHWYVI"
     gene            1544..3172
                     /gene="pgi"
                     /locus_tag="VGM24_12520"
     CDS             1544..3172
                     /gene="pgi"
                     /locus_tag="VGM24_12520"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY4156253.1"
                     /translation="MFPKISPTQTVSWKKLQEQQQLLKGIQMKSLFSSDPGRFDHYSL
                     VFGDILFDYSKNLITSEILGLLQDLARESRLEEAIRAMFSGEKINQTENRSVLHTALR
                     NLSGEPVFSDGKDVMPEVLGVLNQMKSFSEKIHSGEWTGYSGKPIRNIVNIGIGGSDL
                     GPVMVTEALKHYWISGMQAYFVSNVDATQLVETLKKINPEETLFLIASKTFTTQETMT
                     NAFSAREWFLQAAGDEAFITKHFVALSTNESEVIKFGIDPANMFVFWDWVGGRYSLWS
                     AIGLSIVLMVGYPLFEELLYGAHTTDQHFKNTPFEKNIPVIMALIGIWYRNFFGSQTE
                     AILPYDQYMHRFAAYFQQGNMESNGKSIDRNGEPVTYSTGPVVWGEPGTNGQHAFYQL
                     IHQGTVLIPCDFIAPVISHNPLGDHQAKLLSNFFAQTEALMNGRSPEELMEAKVKPEL
                     IPFKFFSGNRPTNSFLIREITPFTLGQLIALYEHKIFVQGVIWNIYSFDQWGVELGKE
                     LAGKILPELHDADKTSNHDSSTNGLINWYKNIRY"
     gene            3256..4356
                     /locus_tag="VGM24_12525"
     CDS             3256..4356
                     /locus_tag="VGM24_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156254.1"
                     /translation="MQFIQKTSEKLRLITRNQYRLFLGILLGIAVIFFVISSFNSGGP
                     FRNGFSGLLSPGDSALNKADSNGRQTVVPVLDTALYNQKLISMANGDSSGRWPVKGGY
                     PAAGAVLPFNRIVAYYGNFYSKKMGILGEYPTDEMLAKLKSDIKKWEAADSTLPVIPA
                     LHYIAVTAQGSPGKGNKYRLRMPFKQIDSLIRMASRINALVFLDVQVGLSTVEEEIPL
                     LEPYLKMPQVHLGIDPEFSMKTGKKPGSAIGTLDASDVNFVIKYLSDLARTNHLPPKI
                     LVVHRFTQAMVTNYKKIRPDSLVQVVMDMDGWGAQSRKFTTYREFIYKEPVQFTGFKL
                     FYKNDYREPNSRMLTPAEVLKLKPRPLYIQYQ"
     gene            complement(4362..4724)
                     /locus_tag="VGM24_12530"
     CDS             complement(4362..4724)
                     /locus_tag="VGM24_12530"
                     /inference="COORDINATES: protein motif:HMM:NF020559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase docking domain-containing
                     protein"
                     /protein_id="HEY4156255.1"
                     /translation="MTEEKDDMISGESAEPDEKKLLDYLRGSLSDAERAQFENRVPSD
                     PFLQDAVDGLHSIENQEDLPKLVNHLNQQLRHHLSNTRTRKGKKDLRFEDWIYWSIGL
                     ILLLALAGFLVLRLLLKH"
     gene            complement(4750..5310)
                     /locus_tag="VGM24_12535"
     CDS             complement(4750..5310)
                     /locus_tag="VGM24_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4156256.1"
                     /translation="MTVPSHINLTDKELLQLFYADHNNEWLGVLLERYTLLLYGVSMK
                     YLKNPEEAKDAVQQIFLKAIPELHKYEVTYFSSWLYMIARNYCLMKLRDKKNPSRPVD
                     RMEDLPATAEEPDFLIRENMLQQLETALNALSGEQKLCINLFYLQKKSYQEIAAETGY
                     SLLQVKSYIQNGKRNLRLLIEKRTGK"
     gene            complement(5399..5950)
                     /locus_tag="VGM24_12540"
     CDS             complement(5399..5950)
                     /locus_tag="VGM24_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY4156257.1"
                     /translation="MNKSTVSHPWHGVPFGSHAPRVVNAIIEIPQGSRAKYEIDKESG
                     LLKLDRVIYSSFYYPVNYGFIPQTYGDDKDPLDILVITSLPVVPLCLMEAKVMGVMQM
                     IDSGDADDKIIAVAARDPSVNHYNNIEELPKHFFDELRHFFEEYKKLENKTVVVEEFG
                     DKTTALKVIEDAIRAYDDSFRNK"
     gene            complement(5953..>6370)
                     /locus_tag="VGM24_12545"
     CDS             complement(5953..>6370)
                     /locus_tag="VGM24_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156258.1"
                     /translation="LKKILLKTLFALASLLLIVACNAGKTFKKAEDAQDAGRQFIRAS
                     LDGDYEKARFYLLNDSVNLLLINKWQRDFDKLDRNTRQQYREASILPISISAINDSVT
                     SYTFSNSFRKDTTTVHIVRTKGEWLVDLKEILNEKR"
BASE COUNT         1588 a         1544 c         1551 g         1687 t
ORIGIN      
        1 acttgtccat aataactggt tttataccgt aatgggttca atcatcatgc catggggtat
       61 tttccggttt ctgttaacag ttcattgcaa tctgaattac ttttattgta gaagaatagt
      121 cccttaccgg attcacgttg attaaatatt tccattttta tgattgattt aagatccgat
      181 accgttaccc gtccggggcc cggcatgttg caggcgatga tgcaggccgc tgtcggcgat
      241 gatgttttca gagaagatcc caccgtgatt gagcttgaac agaccgttgc gggccttttt
      301 gaaatggaag ctgccttgta ttgtgccagc ggaaccatgt cgaaccaggt cgccattaaa
      361 tgccataccc atccgggaga tgaagtgatc tgtgagcagg aggcccatgt gtatatttac
      421 gaaggggggg gtattgcttt taattccggg tcacaggtaa aagccattgc gggcgaccgg
      481 ggcagaatcc aggccggcca gattcccgga tcactcaatc cggatgatgt acataaggca
      541 agaacttcac tggtctgcct ggagaatacc tccaaccggg gaggaggaag ttgttatgac
      601 atcggggaga taaaaaaaat cagagcggtc tgcgatgaaa aaaaattgaa cctacacctg
      661 gatggggcac gcttattcaa tgccctggtt gcgcggaatg aaaagcctcc ggagtacggc
      721 agattattcc acagcatttc cgtttgcttt aacaaggggc tcggctgccc ggtgggaagt
      781 gttttgctgg gcacagccgg tttcatccat caggccagga ggatccggaa ggtattcggc
      841 ggcggtctga gacaggccgg gtacctcgcc gccaccgctt tatatgccct ggagcaccag
      901 gtggaacgac tcgccgaaga tcatgcgcac gcaaagcaga tcgcagcggc gctggaaagc
      961 atgccggttg tccaatccat tcttccggta gagaccaacc ttattatttt tgaattggtg
     1021 ccgggggaag atccgaaact atttgcagcc cggatgaaag cagaaggcat tcttctgctg
     1081 ccggtttcgt ctacccgtct ccgtatggtc acccacctgg atatcagttc cgaaatgacg
     1141 gaccaggtgt gccgggcgat cagaaatcta tcctgatcaa ccctaagtgg ttttacttaa
     1201 ccgcctgttg ttttattatt aaatcacgta ccaatggctt ttttcatcct tggaatagga
     1261 aagcccgtta ctcataacat gcacctccct tccgctttcg gtgtcccgaa ccattttctt
     1321 ttcgtatacc ctgatcccgt tgttttcccg cgtgaggtag taaatcaggg cggtcacgga
     1381 cgggggcact tcatgtaacc actgttcctc attttcctta acaaaagcct ggctcataaa
     1441 gtgtattttt ttgagtatga aaaaatcatt agaaggctaa cttgcattaa ccagatcagg
     1501 ttgtgtattt ttgcatcagg atataaacaa tctaaaccca aatatgtttc ccaagatttc
     1561 cccgacccag acggtttcct ggaaaaagct tcaggaacag cagcagctgt taaaaggcat
     1621 tcagatgaaa agtcttttta gcagtgatcc gggccggttt gatcattatt cccttgtatt
     1681 cggggacatc ctgttcgatt attccaagaa cctgatcacg tcggagatcc tgggtcttct
     1741 gcaggacctc gcaagggaaa gccggctgga ggaagctatc cgggccatgt tttccggaga
     1801 aaagatcaat cagacggaga accgttccgt attgcatacc gccctgcgga atctttccgg
     1861 agagccggtc ttttctgatg gaaaggatgt gatgcccgag gtattggggg ttctgaacca
     1921 gatgaaatcc ttcagcgaaa aaattcattc gggagaatgg accggctata gcggcaagcc
     1981 gatccggaat attgtgaaca tcgggatcgg cggaagcgat ctgggccccg ttatggtaac
     2041 cgaagccctg aagcattact ggataagcgg catgcaggct tattttgttt cgaacgtgga
     2101 tgccacccag ctggtggaaa cacttaagaa aatcaacccg gaagaaactc ttttcctgat
     2161 cgcttccaaa acgttcacga cgcaggaaac gatgacgaat gcctttagcg cccgggaatg
     2221 gttccttcag gctgccgggg atgaagcgtt tataaccaag cacttcgtgg ccttatctac
     2281 taatgaatct gaggtgatca aatttggaat cgatccggcg aacatgtttg ttttctggga
     2341 ttgggtaggt ggcaggtatt cgctttggag cgccatcggg ctttccatcg tactgatggt
     2401 cggttatccg ctttttgagg aacttctcta tggcgcgcat acgacggacc agcattttaa
     2461 gaataccccc ttcgaaaaga atatcccggt aatcatggcc ctgatcggga tctggtacag
     2521 gaactttttt ggctcccaga ccgaggccat tctgccgtat gatcagtata tgcaccgttt
     2581 tgccgcttat ttccagcagg gaaatatgga gagcaatgga aagagcattg accggaatgg
     2641 agaaccggtc acttattcta ccggtcccgt ggtctgggga gagccgggaa caaacgggca
     2701 acatgccttt taccagctga tccatcaggg aacggtattg atcccctgcg attttattgc
     2761 ccctgtgatc agccacaacc ccctgggaga tcatcaggcc aaattgctat cgaacttttt
     2821 cgcacaaaca gaagccctca tgaacggccg ctcgccggaa gagctgatgg aggctaaagt
     2881 aaagcccgaa ctcattccgt ttaagttttt cagcggcaac cgcccgacaa attcatttct
     2941 gatcagggaa atcactccgt ttacgctggg acagctcatt gccctgtatg aacacaagat
     3001 ctttgttcag ggagtcatct ggaatatcta tagtttcgac cagtgggggg ttgaactcgg
     3061 aaaggagctg gccgggaaaa tactccccga actgcacgat gcggataaaa ccagcaacca
     3121 cgattcctcc accaacggcc tgataaactg gtataaaaat atcaggtatt aatccggcca
     3181 agccaaaata aagaatccct gttcccgttt ttgttttcag cgaatccatt tatttcgcca
     3241 ctaagctttt attctatgca attcattcag aaaacctcag agaaactccg tttaatcacc
     3301 aggaatcagt accgtttatt tttggggatc cttttgggaa ttgcggttat ctttttcgtc
     3361 atttcctcgt tcaattcggg tggccctttc cgcaatggtt tcagcgggtt gctttctccc
     3421 ggggacagcg cccttaataa ggcggattca aacggccggc aaacggttgt tcccgtcctg
     3481 gatacggctt tatacaatca aaagctgatc agcatggcca atggggattc ttccggaagg
     3541 tggccggtaa aagggggcta tccggcggct ggagccgtcc ttccctttaa ccggatcgta
     3601 gcttattatg gaaattttta ttccaaaaaa atgggcattc tgggagaata tcccaccgat
     3661 gaaatgctgg caaagctgaa gagcgatata aaaaaatggg aagcggctga ttccacccta
     3721 cccgttattc cggccctcca ttatattgcc gttactgccc agggctcgcc tggaaaagga
     3781 aacaaatacc gattgagaat gccttttaaa caaattgatt ccctgatccg gatggcttcc
     3841 cggatcaacg cgctggtctt cctggacgtg caggtgggcc tgagcaccgt ggaggaagag
     3901 attcccctgc tggaacccta tctcaaaatg ccccaggtgc acctggggat tgacccggaa
     3961 ttctccatga agaccgggaa aaaaccgggt tctgccatcg gaacgctgga tgcatcggat
     4021 gttaattttg taattaaata cctgtctgat ctggccagga caaaccattt gccaccgaaa
     4081 attctggtgg tgcaccggtt tacgcaggcc atggtgacca actacaaaaa aatccggccc
     4141 gattccctcg tgcaggtggt gatggatatg gatgggtggg gtgcccagtc gagaaaattc
     4201 accacgtacc gggaattcat ttataaggaa ccggtacaat tcaccggatt caaactgttc
     4261 tacaaaaatg actaccggga acccaacagc cgcatgctta cgcccgcgga ggttcttaaa
     4321 ctgaaaccca gaccgcttta tatccagtac cagtaaaatg cttaatgctt cagcaggagc
     4381 cggagcacga gaaatccggc cagggcaagt aataaaatta ggccgatact ccagtaaatc
     4441 caatcttcaa accggagatc ttttttccct ttcctcgttc ttgtattgga aagatgatgc
     4501 ctcagctgct ggttcaggtg gttcaccagc ttaggcaaat cctcctggtt ttcgatggaa
     4561 tgcagcccat ccaccgcatc ctgcaaaaac ggatctgacg gaacccggtt ttcaaattga
     4621 gcccgttcgg cgtcggaaag ggatcccctg aggtaatcca aaagcttttt ttcatccggc
     4681 tccgcggatt cacccgatat catatcgtct ttttcttctg tcataacgtt ttattcaacg
     4741 catcatttat catttgcctg ttctcttttc tatcagcagc cgcagattcc gttttccgtt
     4801 ttgaatgtaa cttttaacct gcagcagact atagccggtt tccgctgcaa tttcctggta
     4861 ggattttttt tgaaggtaga acaggttgat acataatttc tgttctccgc tcagcgcatt
     4921 caatgcggtc tccagctgct gaagcatatt ctccctgatc agaaaatccg gttcttccgc
     4981 ggttgccggc aggtcttcca tccggtcaac cggtctggaa ggattttttt tatcgcgcag
     5041 tttcatcagg cagtaattcc gcgcaatcat atacagccag gagctgaaat aagtgacttc
     5101 gtatttatgc aattccggga ttgccttcag aaaaatctgt tgtacggcgt ctttggcttc
     5161 ttccggattt ttcaggtatt tcatggaaac cccgtaaagc agcagggtat agcgttccag
     5221 cagaaccccc aaccattcat tgttatggtc agcataaaac agctgcaaaa gctctttatc
     5281 cgttaaattg atatgggagg gaaccgtcac gctttacaat cagctttctg tagagatgcg
     5341 gaaaacagga aaatgcacag gccggagcat cccgtttgta aaaattaaca ccggaacgtc
     5401 atttgttccg gaaactgtca tcatatgccc ggatggcatc ttcgatcact ttaagggccg
     5461 ttgtcttatc cccgaattct tccacgacaa cggttttgtt ttccagtttt ttatattctt
     5521 caaagaaatg ccgcaattca tcaaaaaaat gtttcgggag ttcttcgata ttgttataat
     5581 ggtttacgct ggggtcccgg gcggccacag caatgatctt atcatccgca tcgccactgt
     5641 caatcatctg catgaccccc atcactttcg cttccatgag gcaaagggga accaccggca
     5701 gggaggttat gaccaggata tccagcgggt ccttatcatc gccgtaagtc tgcggaatga
     5761 acccgtagtt aacgggataa taaaaagaag aataaatgac ccggtcgagc ttgagcagac
     5821 cgctctcttt atcaatctcg tatttggccc gggagccctg gggaatttcg atgatcgcat
     5881 ttacgactct cggggcatga ctgccaaagg gaactccgtg ccagggatgc gaaacggtgc
     5941 ttttgttcat ggttaccttt tttcattgag gatctctttg aggtccacca gccattctcc
     6001 ctttgtgcgc acaatatgga ccgtcgtggt atccttccgg aaactgttgg agaaggtata
     6061 gctggtcacg gaatcgttga tggcgctgat actgatcgga aggatgctgg cctcccggta
     6121 ttgctggcgc gtgttccggt cgagcttgtc aaaatcccgc tgccatttgt tgatcagaag
     6181 caggtttacc gaatcgttaa gcaggtaaaa cctggctttt tcataatctc catccagcga
     6241 tgcccggatg aactgtcttc cggcatcctg ggcatcttct gcctttttaa atgttttccc
     6301 cgcgttgcag gctacaatca acaggaggga agccagggcg aaaagggttt tcaacaaaat
     6361 cttttttagt
//
LOCUS       DASYCU010000625         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4915273, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000625 DASYCU010000000
VERSION     DASYCU010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4915273"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGM24_12550"
     CDS             <1..203
                     /locus_tag="VGM24_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="S1/P1 Nuclease"
                     /protein_id="HEY4156259.1"
                     /translation="NGMVERRMRQSIFAVASFWYTAWVNAGQPPLDRLSHDSLSLGEQ
                     QEYQLLDLEWKKGKVLGRNEEE"
     gene            308..1360
                     /gene="serC"
                     /locus_tag="VGM24_12555"
     CDS             308..1360
                     /gene="serC"
                     /locus_tag="VGM24_12555"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY4156260.1"
                     /translation="MKHNFNAGPSILPKEVFEEASRAILGFRDTGLSILEIGHRTPLF
                     QSVMDETVSLTKELMHLDEDHSVLFLHGGATTQFFQVPMNLLNENETAAYLDCGVWGI
                     KAIKEAKIFGQVNTVASSKEKNYTCIPKGYSIPADSKYFHYTTNNTIEGTEMFEVPET
                     DVPLVADMSSDILSRSMDFNRFALIYAGAQKNIGAAGVNMVVLRRDILGKVNRKLPGM
                     MDYRQHIEAGSMLNTPPVFAIYVCLLTLRWLKNMGGVEGIEKINKAKADLLYKTLEAK
                     PGFELPVAKEDRSRMNVVFRIPEPVLEKKFLDACQQQGMVGVKGHRSVGGFRVSLYNA
                     LPLESVKALTTLINEL"
     gene            1371..>1609
                     /locus_tag="VGM24_12560"
     CDS             1371..>1609
                     /locus_tag="VGM24_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEY4156261.1"
                     /translation="MAIISPFRALRPQPALASKVASPPYDVLNSGEAKASAGGNPYSF
                     LHITKAEIDLPEGTDIHSEKVYRKAEDNLFIFRDQ"
BASE COUNT          425 a          396 c          427 g          361 t
ORIGIN      
        1 taaacggcat ggtggagcgg cggatgcggc aatccatctt tgccgttgcc tctttctggt
       61 ataccgcctg ggtcaacgcc ggccagcctc cgctggaccg gcttagccac gacagtctca
      121 gcctgggcga acagcaggaa taccagttgc ttgacctgga atggaaaaaa gggaaagtgc
      181 tcgggcggaa tgaggaggaa tgatttacgg ggccggcagc accggagccg caaaaaaacc
      241 cttccggaaa atcatccggc agcccggttt tcgtaaattt gcggctcctt caaataaatg
      301 ataaccgatg aagcataatt tcaatgcggg accgagtatt ttaccgaaag aagtttttga
      361 agaggcgagc cgtgccatac tgggtttccg ggacaccggg ctttctatcc tggagatcgg
      421 ccacagaaca ccgctgtttc aatccgtaat ggatgaaacg gtcagtctga cgaaagaact
      481 gatgcatctc gatgaagacc attccgtatt gtttctgcac ggcggggcga ccacccagtt
      541 tttccaggtt cccatgaacc tcctgaatga aaatgaaacc gcggcctatc tggattgcgg
      601 ggtctgggga atcaaggcca ttaaagaggc aaaaatcttc ggccaggtaa atactgtagc
      661 ttcctcaaag gaaaaaaact atacgtgcat tccaaagggc tacagcattc cggccgattc
      721 aaaatatttc cattacacca ccaataacac gattgaagga acggaaatgt ttgaggtccc
      781 tgaaacggat gtgccgctgg tggcggatat gagcagcgat atcctcagcc gttcaatgga
      841 tttcaaccgc ttcgccctga tctatgccgg tgcccagaaa aatatcggtg ctgcaggcgt
      901 taatatggtg gttctgcgca gagacatcct cggaaaagtg aaccgcaaac ttccgggaat
      961 gatggattac cggcagcata ttgaagcagg ttccatgctg aacacccctc cggtttttgc
     1021 gatctatgtt tgtttgctga ccctgcgctg gctaaagaat atgggagggg tggaaggaat
     1081 cgaaaaaata aataaggcca aggcagatct gctctataag acattggagg cgaagccggg
     1141 atttgaactt cccgttgcga aggaagaccg cagtcgcatg aacgtggtgt tccgcatccc
     1201 cgagcccgtt ctggaaaaga aattcctgga tgcctgccag caacagggaa tggttggtgt
     1261 gaaagggcat cgcagcgtcg gcgggttccg ggtctcgctg tacaatgcat tgcctttgga
     1321 aagcgtaaaa gcactgacca ccctgatcaa cgaattataa ttttaacggt atggcaatca
     1381 tttctccctt tcgtgcactc cgcccacagc cggcactggc ttccaaagtg gcttctcctc
     1441 cctacgatgt gttaaacagc ggggaagcaa aagcttcggc cggcggcaat ccctattcat
     1501 tcctgcacat caccaaggcg gaaattgatc tgccggaggg aaccgatatc catagtgaaa
     1561 aagtttaccg caaggcggag gacaatcttt ttattttccg cgatcagaa
//
LOCUS       DASYCU010000626         2808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1584760, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000626 DASYCU010000000
VERSION     DASYCU010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584760"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..615
                     /locus_tag="VGM24_12565"
     CDS             271..615
                     /locus_tag="VGM24_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156262.1"
                     /translation="MALTFQMHFPLLNRVGELRISPYSLVILREGLYSVSVDKVPLIE
                     YYVYFDSPFKTEQIKLKFYKTADGKWYDKTYSEEAELHSPEFGIPEMNAELKSTIDAY
                     ESTHTQMKTFSH"
     gene            complement(760..1431)
                     /locus_tag="VGM24_12570"
     CDS             complement(760..1431)
                     /locus_tag="VGM24_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156263.1"
                     /translation="MRHSSENQIKILYQEFTKLKSYIDKLLFFDLHFGIISFRFPDFD
                     PSLSFFFEDGKTGFLPDILKKERNNPSLHERLFSFSGEYRFSIKPTNSNSSIYSRYIL
                     SKFLSSAPDFDVLIKKRKIQAAPRDAFDKPLLEEANGMINRIEYKLRNEYDKSLTLHG
                     MAVFYKGFSDSFSGQVVLPAKKRKFIELFLYAQGILYSKYIHALKMYYTAQSPGCRDR
                     RKEIG"
     gene            1658..>2808
                     /locus_tag="VGM24_12575"
     CDS             1658..>2808
                     /locus_tag="VGM24_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156264.1"
                     /translation="MNRKQTIKALLKSAFFLFSFTLMVKASDAQFLLNSDSAFKAGSP
                     NSGRIWGYAFGDYYYKAHSDSLVRGGSNQYTGVPKNRSAFAMRRIYLGYDYNISRKFS
                     AELLLAAEDNFTQTNLAGTTTTSGDLLTNNKLSFYIKLLNIRWKNIWKGTDLVVGQVA
                     TPAFPLLSEKIWGYRSIERTIADIRRTPSYDLGVSLQGKFDEKGNYGYNLMVGNGSSA
                     KPEGDNFKWFYGDVFAMFLDKKLVFDLYADYERLNWTPNWHHSRNMIKGYVAYTTPAF
                     TVGVEGFVNNLKNDNVATRIGGGADTLSVVAKGISAYVHGDIVKSKLGFFARFDSFNP
                     ENKIDNNVYLKYAGNTGNYNDPSTKEEFITAGLDITPAKNVHFMPNVWY"
BASE COUNT          759 a          605 c          655 g          789 t
ORIGIN      
        1 atgatagatg gtttttgaac cgtctgccgt ggtaataaac tgcaatgcat ctgccttaga
       61 tcctggtgcc tgcatttgat ttatttccat tttttaaagg tcgtaaataa agaatattga
      121 aaatggcacg gatttctata aaacgggaat ggtataaggt gttctatccc agccggatta
      181 cccgttgatc ctattttggt aacacaaagt taatatgggg tttaggctct agtggcattt
      241 ttgctatagc ttttcaaaat aatcagcact atggcattaa catttcaaat gcattttccc
      301 ctcctgaacc gggtgggaga attgagaatc tctccctatt ccctcgtcat attaagggag
      361 ggcctgtatt ccgtttcggt ggataaggtt cctctgatcg aatactatgt gtatttcgac
      421 agtcccttta agacagaaca gataaagctg aaattttaca aaaccgctga cggtaaatgg
      481 tatgataaaa cgtactccga agaagcagaa ttgcattctc cggaattcgg cattccggaa
      541 atgaatgcgg aattgaaatc aactattgat gcctacgagt ccacgcatac ccagatgaag
      601 accttttcac actagtcagc cttccgccag acttatcact tccgttgttg ttgcggcggg
      661 caggggattt ttaggcccgt tgatgattcc ggggcagaca actgcttccg gacttcattt
      721 ttattatttg aatctccccc ggtaataaga cttatcagtt cagccaattt cttttctacg
      781 atcccggcat ccaggtgact gcgcggtata atacattttc agggcatgga tgtatttcga
      841 gtacaggatt ccctgggcat acagaaagag ttcgatgaat tttcttttct tggcgggaag
      901 caccacctgg cctgaaaacg aatcggaaaa ccccttgtaa aatacggcca taccatgaag
      961 ggtcaggctt ttatcgtact cattccggag tttatattct atccggttga tcatcccgtt
     1021 tgcctcttcc aggaggggtt tgtcaaacgc atcgcggggg gcggcctgta tttttctttt
     1081 tttaatcagc acatcgaagt caggcgccga agaaaggaat ttggaaagga tataccggct
     1141 gtaaatcgaa ctgttggaat tggtcggttt gatactgaag cggtattcgc cgctaaagga
     1201 gaaaagcctt tcgtgaaggg agggattgtt cctttccttt ttaaggatat ccgggaggaa
     1261 tccggttttg ccgtcttcaa aaaagaacga tagcgacgga tcgaaatccg gaaaccggaa
     1321 agagatgatt ccaaaatgaa gatcgaaaaa gaggagctta tcaatatagc ttttgagctt
     1381 agtgaattcc tggtaaagaa tttttatttg gttttctgaa gaatgtctca tactggcatc
     1441 gcctgtccgg attcgccaaa tatacagtgg ggagtttctg caaaaaaccg gaccggatgc
     1501 tggattacct gattgttaac agcggaaggc cggtgccgct ttccaaaccg cgactgaccg
     1561 ggccgaagta atagtttggt aatatatcca taacaatcct ttcacatgcg ttgatttcat
     1621 ttgcatccgg attaattctt ttaaaaatta gctttttatg aatagaaaac aaactatcaa
     1681 agccctgctc aagtctgctt tttttctttt ttcgtttacc ttaatggtta aagcctccga
     1741 tgcgcagttc ctgctgaatt cggattcggc tttcaaggcc ggctccccga attccggaag
     1801 aatatggggg tatgcatttg gagattatta ttataaggca cacagtgact ccctggtaag
     1861 agggggttcg aatcaatata ccggtgtccc taaaaacagg tccgccttcg ccatgcggcg
     1921 gatctacctg ggctatgatt ataatatttc caggaaattc tctgcggaat tattattggc
     1981 tgcggaagac aatttcaccc agaccaacct ggccggcaca accaccacat cgggggacct
     2041 gctgaccaac aacaagctca gcttttatat aaagctgctg aatatccgct ggaagaatat
     2101 ctggaaagga accgatctgg tcgtggggca ggtggccaca ccggccttcc ctttgctttc
     2161 tgaaaagatc tggggttacc ggtccattga aagaaccatc gcggatatcc ggcgtacgcc
     2221 ttcttatgat ctgggcgttt ccctgcaggg taagtttgat gaaaaaggaa attacggcta
     2281 caacctgatg gttggaaacg gaagcagcgc aaaaccggag ggcgataatt tcaaatggtt
     2341 ctatggagat gtttttgcca tgttcctgga taagaaactg gtattcgatc tctatgccga
     2401 ctatgagaga ctgaactgga cgccaaactg gcaccattcc aggaatatga tcaagggtta
     2461 tgttgcctac acgactcctg ccttcaccgt cggcgtggaa ggttttgtga acaatctgaa
     2521 aaatgacaac gtggccaccc ggatcggagg gggcgccgat accctttctg tagttgcaaa
     2581 agggatttcc gcttatgttc atggagacat cgttaaatcg aaattgggat tctttgcccg
     2641 ttttgattcc ttcaatccgg aaaacaagat cgataacaat gtttatttaa aatacgccgg
     2701 caacacaggc aattacaacg atccctccac caaagaagaa ttcataacgg cgggcctgga
     2761 tatcaccccg gccaagaacg tgcattttat gccgaatgtc tggtataa
//
LOCUS       DASYCU010000627         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2308286, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000627 DASYCU010000000
VERSION     DASYCU010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308286"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /gene="dnaE"
                     /locus_tag="VGM24_12580"
     CDS             complement(<1..1799)
                     /gene="dnaE"
                     /locus_tag="VGM24_12580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY4156265.1"
                     /translation="MSTFSHLHVHTQYSLLDGAASIQNLYNKAVKDRMPALAITDHGN
                     MFGAFSFVAEAYKHKNEDGSLKVKPIVGCEFYVVADRFRKTFTKEQKDLRFHQVLLAK
                     NKTGYQNLTKLTSLGYIEGIYSKYPRIDKSLIEKYHEGLIATTCCIGAYVPQTILHEG
                     EEKAEMEFKWWLDLFGEDYFIELQRHNLPEQEKINQFLLKLSKKYNVPVIATNDSHYT
                     DQEDYNAHDILLCINTGEKQSTPGYDDFVNDDVNLKNRRFKFPNDQFYLKTTAEMSEL
                     FRDIPEAIFNTGMVADKVEVLNLKKDILLPAFPIPEAFRIHADSNLNQWEYLKEITYE
                     GSRKRYSELSPEIQERIDFELYTIKTMGFAGYFLIVSDFIRAGREMGVFVGPGRGSAA
                     GSVVAYCIGITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDEGRQKVIDYVVKKYGK
                     NQVAQIVTYGTMAAKMSIKDVARVMDLPLSEANALAKLVPDRPGISLKRVLHAPLTVK
                     EGEKSLEEKEGVGGDDRELIKKIRELYQGTDLRSKVLHEAERLEGSVRNTGLHAAGII
                     IAPDDLANIIPVCTARDSELLVTQIEGSIIEDAG"
     gene            2002..>2461
                     /locus_tag="VGM24_12585"
     CDS             2002..>2461
                     /locus_tag="VGM24_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156266.1"
                     /translation="MKNQLVLFIILVCASGCSVFRPGTTSDGQGASTYQTGSSGRMTS
                     SRSKFINTISTDENTAAGEKSSGVEMNGSSRERYPVTGSSYKKSAGTEKNEKFNELQF
                     KYAILTNTPIELLTNQKLLDFMDEWYGVPYHYGGHDKSGIDCSAFASLLIS"
BASE COUNT          591 a          614 c          597 g          659 t
ORIGIN      
        1 ccggcatctt caatgatact gccttcgatt tgcgttacca gcaattcaga atcccttgcg
       61 gtgcagaccg ggatgatatt ggccagatca tccggagcga tgatgatccc tgcggcatgc
      121 aggccggtat tccgtacgga accttccagg cgttccgctt catgcagcac cttgctgcga
      181 agatcagtgc cctggtagag ttcccggatc tttttaatga gttcgcggtc gtccccgccg
      241 acgccttctt tttcttccag cgatttttcc ccttccttaa ccgtgagcgg agcatgcaat
      301 actctcttga ggctgattcc cggccggtcc ggaaccagtt tcgccagggc attggcttcg
      361 gataaaggaa ggtccattac ccgcgccacg tccttgatgc tcatttttgc cgccatggta
      421 ccgtaggtga cgatctgggc aacctggttc ttcccgtatt ttttcacgac gtagtcaatc
      481 actttctggc ggccttcatc atcaaaatcc gtgtcgatat cgggcatgga ctttctatcc
      541 gggttcagga accgttcaaa aagcaggttg tacttgatcg gatctatatt ggtgatgccg
      601 atgcaatagg ccaccacgct gccggcggcc gaaccgcgcc cgggaccgac aaacacaccc
      661 atctcgcggc cggcccggat gaaatcgctc acgatcagaa aatatccggc aaaacccatg
      721 gtcttgatgg tgtacaattc aaaatcgatc cgctcctgaa tttccgggct gagctcgctg
      781 tatctttttc ttgaaccctc gtaggtgatc tctttcagat actcccattg gttgagattg
      841 ctgtccgcat gaatccggaa cgcttcgggg atcggaaacg ccggaagcag aatatctttt
      901 ttcaggttca gcacctccac tttatccgca accatgccgg tattgaagat cgcttccgga
      961 atatccctga aaagctccga catttcagcg gttgttttca gataaaactg atcgttcggg
     1021 aatttaaaac gccggttctt caggttgaca tcatcattta caaagtcgtc gtaaccgggt
     1081 gtggactgtt tttcgccggt attgatgcaa agcaggatat cgtgcgcgtt gtaatcttcc
     1141 tgatcggtat agtgactgtc gttggtggca attaccggga cattatattt tttagaaagc
     1201 ttcagcagga actggttgat cttttcctgt tcgggaagat tatggcgctg cagttcgatg
     1261 aaataatcct ctccaaacag atccagccac catttgaatt ccatttccgc cttttcttcg
     1321 ccctcgtgta aaatggtctg cggaacatag gcgccgatgc aacaggtggt ggcgatcagg
     1381 ccctcatggt atttttcaat cagggatttg tcgatccggg gatatttgct gtagatccct
     1441 tcgatatacc ccagggaggt cagcttcgtc aggttctggt acccggtctt attttttgcg
     1501 agcaatacct ggtggaaacg gaggtccttt tgttccttgg tgaatgtttt ccggaagcgg
     1561 tccgccacca cataaaattc gcagcccacg atgggtttta ccttcaggct gccgtcctca
     1621 tttttatgct tgtaggcttc tgcgacaaaa gagaaggcgc caaacatgtt cccgtgatcc
     1681 gtaatggcca gggccggcat ccggtccttt acggccttat tatagagatt ctggatggaa
     1741 gcggccccat ccagcagaga gtattgggta tgtacgtgca gatgtgaaaa tgtggacatt
     1801 ggcctggaac ttgcgattgg agtgaaacct gcaattaaca aagaaaaaat tcgttctgtc
     1861 ttttttaacg ggagctatct tctcacatag aacccacaat tattataata atttgataat
     1921 caggagaaaa catctttacc gtaaagagga taattttaac aaaaagaata tctttacagg
     1981 ctcccggtaa gtaaacaaca aatgaaaaat cagttagttt tatttattat actcgtctgc
     2041 gcatccggct gttccgtgtt ccgcccggga accacgtcgg acggacaggg agcatccacg
     2101 tatcaaacag gatcttccgg gagaatgact tcttccagat caaaattcat caataccatt
     2161 tcaacggatg aaaatacggc agcaggggag aaatcctccg gcgtggagat gaatggaagc
     2221 agccgggagc gctacccggt cacgggttcg tcctacaaaa agagcgcggg aacggaaaag
     2281 aacgaaaagt tcaacgagct gcagttcaaa tatgcgattc tcaccaatac cccgatagag
     2341 ctcctgacca accagaagct cctggatttt atggacgaat ggtatggggt tccctaccac
     2401 tacggagggc atgacaaatc cgggattgat tgttctgcct ttgcgtcact cctcatttcg
     2461 g
//
LOCUS       DASYCU010000628         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3033818, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000628 DASYCU010000000
VERSION     DASYCU010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033818"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1158
                     /locus_tag="VGM24_12590"
     CDS             259..1158
                     /locus_tag="VGM24_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY4156267.1"
                     /translation="MKKIFIRILFIFAAFLIFSCQRQSAIPRPDHVIVVVEENHNYEQ
                     IIGSPDAPFINQLARESAVFTNAHGVIHPSQPNYLALYSGSLQGVMGDKCLKDSTPFS
                     TPNLGAGLISKGFSFKGFAETMPEPGFLGCRQDTSASTMGFLYARKHAPWVNWQGKGK
                     NDIPDSASQPMTAFPGDFNQLPDVSFVIPNMDNDMHNIGEPGDSAAIRRGDDWLKAKL
                     QPYIEWAKDHNSLLILTFDEDQFTVKNHMLTLITGAGVIPGKYGERVDHYSLLHTIEG
                     MYDIPVSDSNREAPITYIWKKKG"
     gene            1275..1511
                     /locus_tag="VGM24_12595"
     CDS             1275..1511
                     /locus_tag="VGM24_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156268.1"
                     /translation="MDTIKAMRQEVKRYIETADAKVVKMMHAMLEVDADSSWWESLPD
                     EIKTDVEAALAESEKGEVTPHAEIQKRYKKWVAR"
     gene            1496..1804
                     /locus_tag="VGM24_12600"
     CDS             1496..1804
                     /locus_tag="VGM24_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY4156269.1"
                     /translation="MGCKINWTPRAWLTYEANLLYLQKAWTAKEISNFVILVDKKIAS
                     LSRQPAIGKPRNKKYPQIRFTVIHKRVALIYRHKPSKNEIDLLVFWNAYRNPGKLKLK
                     "
     gene            1994..>2510
                     /locus_tag="VGM24_12605"
     CDS             1994..>2510
                     /locus_tag="VGM24_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY4156270.1"
                     /translation="MKGILFLFCLLTAGLSLVSCQRQTATPNPATPVSTDSLAAAIQD
                     SLPPDTGGAHTYLALGDSYTIGAFVQVQDRYASQTVALLRAAGYPFQDPDIIATSGWT
                     TTNLLNAIKNKTATVPYDVVTLLIGVNNQYQGGSEDNYRQEFTQLLKQSIQLAGDRPS
                     HVIVISIPDYSV"
BASE COUNT          679 a          617 c          617 g          597 t
ORIGIN      
        1 gagggtcacc tgctggtgga gccgatgaag atggaattgt tcgcagaaac ggaaaaggac
       61 ttttatgtga agggagcttt tatcaagctg cattttaaga cgggcgaaaa gggaaagacc
      121 tccggctttc agctcgaaca atttgaaggc agcctgtttg tgaaaaaact ggattaattg
      181 tcagccgtac acttcaacat ccattcggat tcatttaaga tttatatttt ttatcttgca
      241 ggccgaaata caagtttcat gaaaaaaata tttatccgca tcttatttat tttcgctgca
      301 tttttaattt tttcctgtca gcgccagagc gccattccgc gtcccgatca tgttatagtg
      361 gtcgttgaag aaaaccacaa ttacgaacag atcatcggct cgccagatgc cccgttcatc
      421 aatcagctgg cccgggaatc tgctgtgttc accaatgcgc acggcgtgat ccatcccagt
      481 cagcctaatt atcttgcgct gtattccggc agcctgcagg gcgtgatggg ggataaatgc
      541 ctgaaggatt caacgccttt cagcacaccg aacctgggag cgggactgat ttcaaaggga
      601 ttcagcttta aaggatttgc tgaaacgatg ccggagcccg gcttcctggg atgccggcag
      661 gataccagcg cgtccacgat gggttttctc tatgcccgga aacatgctcc ctgggtgaac
      721 tggcagggga aggggaaaaa cgatattccg gattcggcca gccagccgat gacagccttt
      781 cccggcgatt tcaaccagct gccggatgtt tcttttgtta taccgaatat ggataacgac
      841 atgcataata tcggcgagcc gggagattcg gccgccatcc gccgggggga cgactggctg
      901 aaagcaaaac tgcaacctta tattgaatgg gccaaagatc acaacagcct gctgatcctg
      961 acttttgacg aagaccagtt cacggttaaa aatcatatgc tgacccttat taccggagcc
     1021 ggcgtgattc ccggcaaata cggagagcgg gtagatcatt attcgctttt gcacaccatt
     1081 gaaggaatgt acgatatccc cgtatcggat tccaaccggg aggcgccgat cacttatatc
     1141 tggaaaaaaa aaggttgagg gtaagcagta ttcctataac cttcaaaacg agttccggca
     1201 gcatcaccca tggaagtaaa agggtatttt aaacatgaaa ttttaagagt aaatttgtag
     1261 aaacgacagc agatatggat accattaaag ccatgcggca ggaagtaaaa agatacattg
     1321 agacagcgga tgcgaaagtg gtaaagatga tgcatgcgat gctggaagtg gatgcagaca
     1381 gcagttggtg ggagagtctg ccggatgaaa taaagacgga tgtggaagcg gcccttgcgg
     1441 aatccgaaaa aggagaagtg actcctcacg cggaaataca aaagcggtat aagaaatggg
     1501 ttgcaagata aactggacac ccagggcatg gctcacctat gaagccaacc tactctacct
     1561 ccaaaaagca tggacagcca aagagatcag caactttgta atattggtgg ataaaaagat
     1621 cgccagtttg tccaggcaac ctgcgatagg caaaccccga aataagaaat accctcagat
     1681 acgctttacc gtcatccaca aacgggtggc cctgatctat aggcacaagc cgtcaaagaa
     1741 tgaaatagat ctgttggtct tttggaatgc ttaccgcaat cccggtaaat taaaactaaa
     1801 ataacttctt cgctactgac cacggtctct ctggttgtgc atctgcgaat caagatctct
     1861 ctggttgttt gttcttgtat tcttgcaata agcggatgct tgtttatcct tatatccggg
     1921 aaaatattgg ctttattttc tcgctccacc gggtccgcta aaccgcaacc ccgttttccc
     1981 cgtatatagg aaaatgaaag ggattttgtt tcttttctgc ttactcaccg ccggcctctc
     2041 ccttgtctcc tgtcaaaggc agaccgcaac ccccaatccg gcaacgccgg tcagcaccga
     2101 ttccctggcg gctgccattc aggatagcct gcccccggat acgggagggg cccataccta
     2161 tcttgcgctg ggagattcct acaccatcgg ggcatttgtc caggttcagg accggtacgc
     2221 ttcccagacc gtcgccctgc tccgggccgc agggtatccc ttccaggatc cggatattat
     2281 cgccaccagc ggctggacaa ccaccaatct cctgaacgcg ataaaaaaca aaacggcgac
     2341 ggttccctat gatgtggtta cactcctgat cggggtgaat aatcaatacc agggtgggag
     2401 cgaagacaat taccgccagg aattcacgca gctcctgaag cagtccatcc agctggccgg
     2461 agaccggcct tcccacgtga tcgtgatctc gatcccggat tattccgtta
//
LOCUS       DASYCU010000629        14086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1008628, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000629 DASYCU010000000
VERSION     DASYCU010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008628"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1472)
                     /locus_tag="VGM24_12610"
     CDS             complement(111..1472)
                     /locus_tag="VGM24_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156271.1"
                     /translation="MKKTFIIFCLALCLLLMFSSSLSILESLSVAVVVYAFLEFLHDL
                     GKRIVIMDISILALFLTCILMPVIFYHVYTRENHLARLWVKYMPVESDDYFSFALPAV
                     FAMAWALRIRIKKLRFRKTPLVYLENVKRSLSKKPTIGISLIGIGLVSGLLDFLAPDS
                     LKQIFYLADHLTYVGVFYIIYSPFQYKRIIVPGVIALMLGQSVVTGMFGEMIYILACS
                     LTLILLGSKISFYAKLTFAVLGIFMILVLQSIKMEYRHQNWELGKGADPVYFAALIAS
                     RVTDVSSLMDPDNMFFTAVRMNQGWLVAVTMKRVPARFPFGYGETIWKSVASSFIPRF
                     IWPDKPKAGGKENLLRYWGFIIVGYSMNIGTLGEAYANFGKFGGVAYMFAYGFFFNFF
                     LSSILKLAEKRPTIILWLPFLFFYSISVETDLFTTMGALVKSMIFTWIIFKFYEIAFH
                     IEL"
     gene            complement(1469..3808)
                     /locus_tag="VGM24_12615"
     CDS             complement(1469..3808)
                     /locus_tag="VGM24_12615"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4156272.1"
                     /translation="MVTIAGSTKLWAFNMAEQMEKYGLLDKLMTSYSYSKNTFARKFI
                     RRIDKENIPLSKIQTNLLLAFPIGTFREKAYWWNILFDRWVAWNLRKSDSKIFIGWSG
                     MSLHSIRAAKKRGMVTIVERGSSHILTQNKILHEEYKRYNRDFSINASLIRKELKEYD
                     EADYIAVPSYFVRDSFIEQGIPKEKLFLNPYGANSYFKASVSKKKDNKFRIVYLGTLS
                     IRKGLIYFFEAIKELQVPMESYEVLLIGKIEPELEKIVSSFRQENWRVMGHIDHYQLP
                     DILATCDVGVQPSVEEGLSMVIPQMMACGVPVIITPNTGGQNIVNDGVNGFVVPIRDA
                     TEIRNKITWSFDHPRELETMKQNAAASIQDNFTWDAYGSRYHQFLKKLLLLAPPFRGY
                     PDKGKSIAIAIYTHPEYYPPLLNAIDELSQNTDQLFVISRNLKINNWKYPEGVELISS
                     GELIDIRVAEQTSIAWKFRSFLRFTLDFYKLLLRKKHEWIICQDPISLLSYRLIHRLI
                     WYKPRLWYHNHDIVERSAVRKYSVGYFSVVSEKRFFKHIDLFSLPSVERLAAFPFEQL
                     KRPYFIIPNYPSTRRVGYETISKTDPAQVLKLIYQGHLGNDHGLEAFINFIEQEKDIT
                     LTLIGPGNPEFILHVKQLIIQKRLHDRVFLRRPVPYASLKEITAAHHIGLAVHEPVNI
                     AFRTAAMASNKIYEYAASGLPVIYYDDEHYKKYLSIYPWAFSTDLSMESIRNAIHFIR
                     SNYEELSARALKDFRTKLNFGSVYKPVMEYMKAFNQDAK"
     gene            complement(3810..4838)
                     /locus_tag="VGM24_12620"
     CDS             complement(3810..4838)
                     /locus_tag="VGM24_12620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4156273.1"
                     /translation="MKILLAGSDFEYGIEQYYIKYLRQLGAEVIHYPAPDRVIQQRSK
                     NIISRILFKTRMRTGYAIVNRELIRLAEENDPDVIWIFKGMEIYPSTLRHLGKKYRIA
                     NYNPDHPFIISSRGSGNDNVTGSVGLYQLHFCYNRNLQKQIEKKYGIPTVFLPFGFEL
                     SPEDYLTARQEPEINRICFIGNPDRIRIHLLTLLAKNGFGVDVYGHGWDKTPLRKFPG
                     INISDAVYGQGFWKRLRQYRVQVNIFRKHNAGSHNMRSFEIPAVGGIQLAPWSEEQAD
                     FFSEGKEIFFYRDEDDLLKQVKNLLLATAETANGYREAARKRSLDAHYRYQDRALTVY
                     ENFKILIG"
     gene            complement(4854..6008)
                     /locus_tag="VGM24_12625"
     CDS             complement(4854..6008)
                     /locus_tag="VGM24_12625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4156274.1"
                     /translation="MTDLIVVSHACFTAINRNLYHLFEKDGWKLEIVVPKTLIFSSGE
                     KKAEPPQPGDPPLHYLDLIGKNPRTYYFKNLIGLLDEKRPRIILLDNDPVSRLSIQLG
                     RWTKKNNAWLFCVSNENLPLDISSAVTRRGWKAAPAAILKRMMLRETKALVDGIFTVN
                     HDGEKLFKQEGYVNVRHMPLGFDPVYFYPDREARERIRKELALNGCVVSYFGRLTPEK
                     GIHILIQALGELKQYAWQLLMDSFDPSTSSYHAEINRCLAEAGIADRVVFINPSHTGI
                     AAYMNAADIVVVPSVTTPAWKEQYGRVAAEAMACGKTVIASDSGALPDLLNGYGYLFE
                     EGNAARLKDILEKLLSGSGNGLLNESAISGYARENLSIQKQKTVIEEAFR"
     gene            complement(6005..6949)
                     /locus_tag="VGM24_12630"
     CDS             complement(6005..6949)
                     /locus_tag="VGM24_12630"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY4156275.1"
                     /translation="MSRILITGGFGFVGGRITRRLAAEHEVWVSSRNKVQDMILRLHG
                     NVRCFDHALLLSEDTFPAFIDTVIHLAALNEQDCVRYPSEAIRVNVDETRKILENSIT
                     RGVEKFIYFSTAHIYGAPLQGSISEETLAFPVHPYAITHRAAEDYVVAATKQKRIQGA
                     VFRLSNSFGAPVSPLVNRWTLLANDLCRQAVEKGKLTLNSNGCQYRDFICLSDVEEVV
                     TRMVNRAKENKHIIYNLGSGISTRVMDMADGIVRAAVTVLKKNIPLELPAGCPPSSEP
                     ELFYSVDRLLSEGYRIQNDVGMELERLMQFCKENFSRH"
     gene            complement(6946..7392)
                     /locus_tag="VGM24_12635"
     CDS             complement(6946..7392)
                     /locus_tag="VGM24_12635"
                     /inference="COORDINATES: protein motif:HMM:NF017345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY4156276.1"
                     /translation="MGVIKEVVLTPLRIIPGDSGEVLHALKQNEISFQGFGEAYFSTV
                     RKHAVKGWKKHRRMLLNLVVPSGVIRFVLYDDRPESKSFQTIQEVVLSKENYQRLTVP
                     PGVWMAFQGMSDELNILLNIASIPHDPAEADSLPLRNGLIPYPGFS"
     gene            complement(7380..8486)
                     /gene="rfbG"
                     /locus_tag="VGM24_12640"
     CDS             complement(7380..8486)
                     /gene="rfbG"
                     /locus_tag="VGM24_12640"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEY4156277.1"
                     /translation="MFNNIYKDKKIIVTGNTGFKGSWLSLWLHYLGAKVTGISKDIPS
                     TPSLFEELDLAKKTEHHFANICDGSRMKEIFAQVQPDFVFHLAAQPIVSVAYDDPVGT
                     FNTNTIGTANILEALRTLEGPCHAVIITSDKCYDNLEWIWGYREEDALGGKDPYSASK
                     GAAELVIKTYYHSYFKKPGSRTRLTTVRAGNVIGGGDWALNRIVPDCFRSWNRQKAVQ
                     VRNPNSTRPWQHVLEPLSGYLRAGQILAETNGEINGEPFNIGPAADQNHTVLELLLAV
                     NKFWNAGSLKEHFEIVENTGFHEAGLLKLNCDKALYYLQWRPALDFAATARFTGEWYN
                     HYYNNNKEDISGYSLRQIEEYAEAARKKSISWVL"
     gene            complement(8490..9341)
                     /locus_tag="VGM24_12645"
     CDS             complement(8490..9341)
                     /locus_tag="VGM24_12645"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEY4156278.1"
                     /translation="MNIRKLLYFHFNSSLARSILALFFKEDHFYKIPFGAIKGEKLYY
                     RKDINFHSTMGVWEKDSLEVLNKLFLRFGLNQPDIVIADVGSNIGYYCLFFSKYLDPS
                     ARIFAFEPSTSILPVLRKNLVVNNVSNVKVLELALADHTGNEEFFLGQHHHESSLLRE
                     WSSNAVSGTKTTVATVTLDYFFENFNQGRYPDLIKMDIEGGGIFALKGCVNCLSRKRP
                     FILIESHNPAEDEAVSHVLLQYNYEALRVNNNKWIKNKETHYPDPNGVWGTMLLMPAE
                     KKPDFIN"
     gene            complement(9341..10111)
                     /gene="rfbF"
                     /locus_tag="VGM24_12650"
     CDS             complement(9341..10111)
                     /gene="rfbF"
                     /locus_tag="VGM24_12650"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEY4156279.1"
                     /translation="MKVLLLAGGFGTRLSEETDIRPKPMAEIGGKPILWHIMKGYSHY
                     GYTEFVVLLGYKGYYIKEYFANYFLHQSDVTIDLSTNELQVHNNTSEPWKVTLLETGQ
                     DTMTGGRIKRAKEFIGKEPFLLTYGDGVSDINLKELVSFHQKHGKYITMTAVQPEGRF
                     GALETEGDRVDSFLEKPRGDGNWINGGFFACQPEVLDYIAGDDTVFEQGPLQKLAMEG
                     QLYNYRHSGFWKCMDTLRDKKMLNELWEGGHAKWKTWI"
     gene            complement(10116..11264)
                     /locus_tag="VGM24_12655"
     CDS             complement(10116..11264)
                     /locus_tag="VGM24_12655"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4156280.1"
                     /translation="MIPGSEERMNRIYFKGLNGIRAMAAFIVITFHIDQFLRLFGLKS
                     FGYHNTGMAGFGVTLFFVLSGYLITYLLLTEKKKYEKVDLLKFYKRRILRIWPIYYLA
                     VGFVILLILFKFLIVPEKHLGLNLFFYIFLLSNVAYSGILPITPLWSIGVEEQFYLFW
                     PLLLNRAGNVFRALLGIIVIYLAIKIGFRIFENGPIYSVIYLTSFDCMAIGGIMANYV
                     FRKHSILKLFYTPLLQVIAWSFLAVSIFYKPVHFSSLFDPELHAVVYAIIIVNVSTNS
                     RSLLNLENKLLNFLGRISYGLYVYHMLIIWLLSRFLKPYIPAIPGRWLPYITVYGLVY
                     AITIFVAYLSYHYFESYFLKLKTRFAKIHSTNEARPDLPDLKEPILIQ"
     gene            complement(11267..12400)
                     /locus_tag="VGM24_12660"
     CDS             complement(11267..12400)
                     /locus_tag="VGM24_12660"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY4156281.1"
                     /translation="MKTQNSRIPAIDGLRTLAVLGVLWTHVWMFFKNIPMKVGGLDLN
                     RGMAFGRHGVDLFFVISGFCMYLMYASRIDHFSASNFFGFLKKRWLRIAPAFYMIVVF
                     DSFLLLYHTGIFPLKQMSYHLLFINIFLDQYDFAPHYWSLATEFHFYLLFPFLFLRLR
                     SQRELSLRVFALMAVCLIFRLILFYNHTNDILEKETITSTAIWYRFIEFGFGIIAAQL
                     YVLKRVLPRWAIGSSGFILGLLISYAGKIIITTEFVRHFGTKAFIFRAFAEPIMTFGF
                     GWMLISLITSGGIFTRVLSSRPFLFLGKISYSFYLWHWFVAHLVSNWLIAWGGMNLKT
                     FYLSLLISLVVAIPIAFLSFRLFEAPYFKRNPKLKKMAYVEVV"
     gene            complement(12397..13566)
                     /locus_tag="VGM24_12665"
     CDS             complement(12397..13566)
                     /locus_tag="VGM24_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156282.1"
                     /translation="MDAGFLSNKVILILSPQSWGKMLLAKHHYAIELASRGNEVYFLN
                     PPDNDHWSWKRATQRISVLKSADHSNLFLIDQQLFFPYKIIFHARRLYNFFIRKQIAD
                     ILRVIGKPVDVIWSFDLGNLFPLRYFNPSVYKVFHPLDEPRTPEAIRASAGADVLFSV
                     TGEILARYKYPLSRHLINHGISDEFIKEQPDHRNGDRIQVGFSGNLLRRDIDRDTLLS
                     IIRDNQELDFNFFGSYEIGQSNIGAGMDNRTAGFIKDLKILANVKLHGVLDSAALAAA
                     LNEMQVLLICYDIEKDQSRGTNYHKVMEYISTGKVIVSNNITAYAGEPALVRMVAERN
                     NNKNLGTLFLDTVRNLDTHNSPELFHYRVNFARNNTYKRQLDRIEELIRQTRKPS"
     gene            complement(13580..>14086)
                     /locus_tag="VGM24_12670"
     CDS             complement(13580..>14086)
                     /locus_tag="VGM24_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY4156283.1"
                     /translation="ALADKCRMYSHHGALKKHQHQMEGINSRLDGLQAAILSAKLPHL
                     GDWTEKRIQSAALYDKWLADIPGIILPRQRPDTKHTWHLYVIRSGQREKLAAYLSGKG
                     IETAVHYPVALPNLVVYQYLGYKPSDFPVATRLQNEILSLPMFPELTEEMIAYVADGV
                     RSFIASLR"
BASE COUNT         3766 a         3479 c         3205 g         3636 t
ORIGIN      
        1 aaactgattc ctttcctttt ccctgatgct gccggtaatc tcctggaata gtttctcaat
       61 actaaagtaa atcgggtggg ggtgacgata aaagaaatga atcgatttca tcaaagctcg
      121 atatggaatg cgatttcata aaatttgaaa ataatccagg taaagatcat ggatttaacc
      181 aatgcaccca tcgttgtaaa tagatcggtc tccacgctga tggaatagaa aaaaagaaag
      241 ggcagccaga gaatgatggt gggtcttttt tcagccagct ttaaaatgga ggataaaaag
      301 aaattaaaaa aaaatccata cgcgaacatg taggcgaccc ctccgaattt tccgaagttc
      361 gcatacgctt cccccagcgt accaatattc atgctgtatc cgactatgat gaatccccag
      421 tatcgtaaga ggttctcctt tccccctgcc ttgggtttat ccggccatat aaatcgcggt
      481 ataaaggagg aggcgactga cttccagatg gtttctccat atccaaacgg gaacctggcc
      541 gggacccgtt tcatggtcac tgccaccagc cagccctggt tcattctgac ggccgtgaaa
      601 aacatattat cgggatccat taaagaactg acatccgtta cccggctggc gatcaacgcc
      661 gcaaaatata ccggatctgc gccttttccc aactcccagt tctgatggcg gtattccatc
      721 ttaatggact gaagcaccag gatcataaat atccccagta cggcaaaggt gagcttggca
      781 taaaagctga ttttcgatcc cagcaaaatc aaggttaggg agcaggccag gatatagatc
      841 atttccccga acattccggt caccacggat tgccccagca tcagcgcaat caccccgggt
      901 acgatgatgc gcttatattg aaatggagaa taaataatat agaacacccc cacataggtc
      961 aggtgatcag ccagataaaa gatctgcttt aaactgtccg gggctaaaaa atcaagaagt
     1021 ccggaaacaa gaccgattcc aataaggctt atcccgatgg tgggtttttt gctcaggctc
     1081 cgttttacat tttccagata aacgagtggc gttttccgga agcgtaattt tttaatgcgt
     1141 atccgaagcg cccaggccat cgcaaatacg gcgggcaggg caaaggaaaa atagtcgtcc
     1201 gattcaaccg gcatatattt tacccataac ctggccagat gattttctct cgtatagacg
     1261 tgataaaaaa taacgggcat taggatgcag gtcaggaaga gggccagaat ggaaatatcc
     1321 ataatgacga tccgtttgcc aagatcatgt aaaaattcca gaaatgcata taccaccacg
     1381 gcaacactca gggattccag gatgctgagc gagcttgaaa acataagcag caaacacaaa
     1441 gccaggcaga atataataaa tgttttcttc atttggcgtc ctgattaaag gccttcatat
     1501 actccattac cggtttataa acggacccaa aattgagttt tgtacggaag tctttcaatg
     1561 ccctggcaga aagctcttcg taatttgaac ggataaagtg gatcgcgttc cggatacttt
     1621 ccatggaaag gtcggttgaa aacgcccagg gatagatgga aagatatttc ttatagtgct
     1681 catcatcata gtaaataacc ggcaggcccg aagccgcata ttcatagatc ttgttggaag
     1741 ccatggcagc ggtacgaaat gcgatattga ccggttcgtg cacggctaat ccgatgtggt
     1801 gggcggcggt gatttctttc agcgaggcgt agggtaccgg ccgccggaga aatacgcggt
     1861 catgcaggcg cttttgtatt ataagttgct tgacgtgcag tatgaattcc ggattgcccg
     1921 gcccgatcag tgtgagcgtt atgtctttct cctgttcaat gaaattaatg aaggcttcca
     1981 gtccgtggtc attccccaag tggccctggt aaatcagttt cagcacctga gccggatccg
     2041 tttttgaaat ggtctcgtac cccacccgcc gggtagacgg ataattggga ataataaaat
     2101 atggcctttt cagctgttca aacgggaagg ccgccagtct ttccaccgaa ggaagcgaga
     2161 acaggtcgat atgtttaaaa aaacgctttt ccgaaaccac agagaaataa ccgacagaat
     2221 attttcttac tgccgatcgt tcaacaatat catggttatg ataccatagc ctgggtttgt
     2281 accagatcag ccgatggatc agccggtagg aaagcaggga aatcgggtcc tggcagatta
     2341 tccattcatg ctttttgcgc aggagcaatt tataaaagtc cagggtgaac cggagaaaag
     2401 aacggaattt ccaggcgatg gatgtctgtt ccgccacccg gatgtcaatc agttcgccgc
     2461 tgctgatcag ttccactcct tccgggtatt tccagttatt tattttcagg ttcctggaaa
     2521 tgacaaacaa ttgatcggtg ttctgcgaaa gctcgtctat cgcattcagc agcgggggat
     2581 aatattccgg gtgggtatat atagcgatcg caatgctttt ccccttatcc ggatagccgc
     2641 ggaacggagg agcgagcagc agcaatttct taaggaactg atggtaccgg ctgccatagg
     2701 catcccaggt aaaattatcc tgaatggatg cggctgcatt ctgcttcatc gtttccagtt
     2761 cccgggggtg atcgaatgac caggtaatct tattcctgat ttccgttgca tcccggatgg
     2821 gcaccacaaa tccgttcacg ccgtcattga caatattctg accacccgtg ttcggcgtga
     2881 tgatcaccgg aaccccgcaa gccatcatct ggggtatgac catgctcagc ccttcttcaa
     2941 cggatggctg aacgcccaca tcacaggttg ccagtatatc cggcagctgg taatgatcaa
     3001 tatgacccat aacccgccag ttctcctgcc tgaaagatga aactattttt tccagttccg
     3061 gttcgatctt tccgatcagc aacacctcat aggattccat tggtacctgc aactccttaa
     3121 tggcctcaaa gaaataaatt agccctttgc gaatggacag cgttcccagg taaacgatcc
     3181 ggaatttatt atcctttttt tttgaaacag acgctttaaa atagctattg gccccatagg
     3241 gattcaggaa aagcttttct ttggggattc cctgttcaat aaaactgtcc cggacaaaat
     3301 aagaaggaac ggctatgtaa tccgcctcat catattcttt tagttccttc ctgatcagag
     3361 aggcattaat ggaaaaatcg cggttatacc gcttatattc ttcgtgcagt atcttatttt
     3421 gagtgaggat atgggaagaa cctctttcca cgatggttac cattcccctt ttttttgccg
     3481 cccggatgct gtgcaggctc atgccgctcc agccgatgaa tatcttactg tcgctttttc
     3541 tcagattcca ggcaacccag cggtcgaaca ggatattcca ccagtacgct ttttcccgga
     3601 atgttccgat cggaaatgca agcaggaggt tcgtctgtat cttggaaagc gggatattct
     3661 ccttgtcaat tctccttatg aatttccgtg caaaggtgtt tttggaatag gaataggaag
     3721 tcatcagctt atccagcaga ccgtactttt ccatctgttc agccatattg aaggcccata
     3781 atttcgtact gcccgcgatc gttaccattt caaccgatca atattttaaa attttcatag
     3841 actgtcaaag cccggtcctg gtaacggtag tgagcatcca gcgaccgttt cctggctgcc
     3901 tcccggtacc cgttcgccgt ttccgcggta gccagcaata aattcttcac ctgctttagc
     3961 aggtcgtcct catctctgta gaagaatatt tctttccctt cagaaaaaaa atcagcctgc
     4021 tcctcactcc agggcgccag ctgaatgccg cccacggcgg ggatctcaaa actgcgcatg
     4081 ttgtgggagc cggcattgtg cttccggaag atattcacct gtacccggta ttgccttaac
     4141 cttttccaga atccctgtcc gtatacggca tcggaaatat ttatacccgg aaatttccgg
     4201 agcggggtct tatcccagcc atgaccgtaa acatccaccc cgaatccgtt tttcgccaat
     4261 agtgtcagga ggtggatccg gatccggtcc gggttaccga taaaacaaat ccggtttatt
     4321 tccggctcct gccgggcggt gagataatcc tcgggggaaa gttcaaaacc aaatggcaga
     4381 aacaccgtgg gaatcccata ttttttttca atctgctttt gcaagttccg gttatagcaa
     4441 aagtgcagct gatataaacc aacactgccc gtgacattat catttccact accccggctg
     4501 gaaattataa acggatggtc cgggttataa ttggcgatcc ggtatttttt tcccaggtgc
     4561 cgaagggtgg aaggatatat ttccatcccc ttgaagatcc agatcacgtc cggatcattt
     4621 tcttccgcca gccggatcag ttcccggttt acgatagcgt aacccgtgcg catacgggtt
     4681 ttgaaaagga tccggcttat tatatttttt gagcgctgct ggatcacccg gtcaggcgcc
     4741 ggataatgaa tgacctccgc tccaagctgc cggagatatt ttatatagta ctgttctatt
     4801 ccgtattcaa aatcacttcc tgccagcaaa atcttcatag ataaaataag gacctaccgg
     4861 aatgcttcct cgatcaccgt tttctgtttc tgaatgctta aattttccct tgcgtacccg
     4921 gaaatagcgg attcatttaa aagaccattg ccggaacccg atagtaattt ttccaggatg
     4981 tctttcagcc gcgccgcatt cccttcttca aacaaatatc catatccgtt cagcagatcc
     5041 ggcagcgcgc cgctatcgga ggcgatcact gtttttccgc aggccatggc ttctgcggcc
     5101 acccggccat attgttcctt ccatgccgga gtggtcacgg aaggtactac cacgatgtcc
     5161 gccgcattca tataagccgc aattcccgta tgggacggat tgatgaacac cacccggtca
     5221 gcgatacccg cctctgccag acagcggttg atctccgcgt ggtaagagga ggtagacgga
     5281 tcaaatgaat ccatcaggag ttgccacgcg tactgtttca attcgcccag tgcctgaatc
     5341 aggatatgaa ttcccttttc cggtgtgagc cttccaaaat aagaaacgac gcatccgttc
     5401 aaggccaatt ctttccgtat ccgctccctg gcctcgcggt ccgggtaaaa atagaccggg
     5461 tcaaaaccca gcggcatatg ccttacattc acatatcctt cctgtttgaa taatttctca
     5521 ccgtcgtggt taacggtgaa gataccatcc accagtgcct ttgtttcccg aagcatcatt
     5581 cgcttcagaa tggccgccgg cgccgctttc cagcctctcc gggttactgc agaagagata
     5641 tccaggggca ggttttcgtt cgaaacgcaa aataaccagg cattgttttt ctttgtccac
     5701 ctacccagct gaatgctgag gcggctcacc ggatcgttgt ccagcagaat gatccggggg
     5761 cgtttttcat ccagcaaccc gatcaggttt ttaaaataat aggtacgggg attctttccg
     5821 atcaaatcca gataatgcaa tggcgggtct ccgggttgcg gtggctccgc ctttttttcc
     5881 ccggaagaaa aaataagcgt tttcggaacc acgatctcca gcttccatcc gtccttttca
     5941 aacaaatgat acaggttccg gttgatggcg gtgaaacagg cgtgggagac gacgatcaga
     6001 tcagtcaatg cctggaaaaa ttttctttac agaattgcat aagtctttcc agttccatgc
     6061 caacatcatt ctgaatccgg tagccttccg ataacaggcg gtctaccgaa taaaataatt
     6121 cgggttccga ggaaggcggg cagcctgccg gcaattccag cggaatattt tttttgagta
     6181 cggtaactgc agcgcgcaca atgccatcgg ccatatccat taccctggtg ctgatacccg
     6241 aacccagatt ataaataatg tgtttgttct cttttgccct gttgaccatc cgggtaacca
     6301 cttcttccac atcggacaaa catatgaaat cgcggtactg gcatccgttg gaattcaggg
     6361 tcagtttgcc cttctccacg gcttgtctgc ataaatcgtt tgccagcagg gtccagcggt
     6421 tgaccagcgg ggaaaccggt gcgccaaatg aattggaaag gcggaacacg gcgccctgta
     6481 tccgtttttg ttttgttgcg gccaccacat aatcctcggc tgcccgatgg gtaatggcat
     6541 agggatggac gggaaaagcc agggtttctt ctgaaatgct cccctgtaag ggtgcgccat
     6601 aaatatgcgc ggtggaaaaa taaataaact tctccacccc gcgcgttatg ctgttctcca
     6661 ggattttgcg tgtttcatcc acattcaccc gaatggcttc cgaaggatac cggacacaat
     6721 cctgctcgtt cagtgcagcc agatgaatga ccgtatcgat gaaagcagga aacgtatctt
     6781 ccgataataa gagcgcatga tcgaagcaac gcacgtttcc atgcagacga aggatcatgt
     6841 cctgcacttt attcctggag gacacccata cttcgtgctc cgctgcgagc cgccgggtga
     6901 tccttccccc aacaaatccg aatccacctg taattaaaat cctgctcatg aaaaacccgg
     6961 gtagggaatc aacccgttcc gcaggggtaa gctgtccgcc tcggccggat catgcggaat
     7021 gctggcaatg ttcagcaata tgtttaattc gtccgacatt ccctgaaagg ccatccagac
     7081 accgggcggc acggtcaacc gctgataatt ttctttggaa aggaccactt cctgaatcgt
     7141 ttgaaagctc ttgctttcgg gacggtcgtc atacaacaca aaccggatga cgcccgaagg
     7201 cacaaccagg ttgagtaaca tccgccggtg ttttttccag cccttgaccg cgtgtttgcg
     7261 aaccgtggaa aaataagctt ccccgaatcc ctgaaaagaa atctcatttt gttttaacgc
     7321 atgaagtact tctccggaat ccccgggaat gatgcgcagc ggggtgagca ccacttcctt
     7381 tataacaccc atgaaattga ttttttccgg gcggcttccg catattcctc aatctggcga
     7441 agggaataac cgctgatatc ttccttattg ttgttataat aatggttata ccattctccc
     7501 gtgaaccggg ccgtggctgc aaaatccagg gccggccgcc attgcaggta gtataatgcc
     7561 ttgtcacaat tcagtttgag aagacccgct tcatgaaaac cggtgttttc cacaatttca
     7621 aaatgctctt tcagggaacc ggcgttccag aatttattga cggcgagtaa caattcaagc
     7681 acggtatgat tctgatccgc cgccggcccg atattgaagg gttctccatt gatctccccg
     7741 ttcgtttccg caagaatttg gccggccctc aggtaaccgc tcaatggttc cagcacatgc
     7801 tgccaggggc gcgtggaatt gggattccgg acctgaactg ccttttgccg gttccaggaa
     7861 cggaagcaat ccggcacgat gcggttaagc gcccaatcgc cgccgccaat cacgtttcct
     7921 gcgcgaaccg tcgttaaccg cgtacgcgat ccgggttttt tgaaataaga atgataatac
     7981 gttttgatga ccagttctgc cgctcctttg gaagcgctgt acggatcctt tcctcccagc
     8041 gcatcctctt cccggtagcc ccatatccat tccaggttat cgtaacattt atcgctcgtg
     8101 atgattacgg catggcaggg cccttccagc gttctcaggg cttcgagaat atttgccgtg
     8161 ccgatggtat tggtattgaa tgttcccacg ggatcgtcat aagcaaccga aacgatgggc
     8221 tgggccgcca ggtgaaaaac aaaatcgggc tggacctgcg cgaagatctc tttcatccgg
     8281 gacccgtcgc aaatgttggc aaaatgatgt tctgttttct tggcgagatc cagttcttca
     8341 aaaagggaag gggtgctggg aatgtctttg gaaatgccgg tcactttcgc acccagataa
     8401 tgcagccaga gactaagcca ggagcccttg aaaccggtgt ttccggttac gatgatcttt
     8461 ttatccttgt aaatattgtt aaacatagat caattgataa aatcgggttt tttttctgcc
     8521 ggcatcagta acatggttcc ccaaacccca ttggggtcgg gataatgagt ctccttattt
     8581 ttgatccact tattattatt caccctcagc gcttcataat tatattgcag cagcacatgg
     8641 cttaccgcct cgtcctccgc cgggttgtgc gattcaatca gtataaacgg tcttttccgg
     8701 gaaaggcaat ttacgcatcc tttcaaggca aatattcccc ctccctcgat atccattttg
     8761 atcaggtccg ggtaacggcc ctggttgaaa ttttcaaaga aatagtccag ggttaccgtg
     8821 gccaccgtgg tttttgtgcc ggaaacggca ttgcttgacc attccctcaa taaggaggac
     8881 tcatggtgat gttgcccgag aaagaattct tcgttcccgg tatgatcggc tagggcaagt
     8941 tccagcactt ttacattgga aacattgttg acgaccaggt tctttctcag caccggcagg
     9001 atggaggtgg atggttcaaa agcgaatatc cgggcggagg gatccaggta tttcgaaaag
     9061 aaaagacaat aatagccgat attggagccc acgtctgcaa tcacgatatc cggttgattc
     9121 aatccaaaac gaaggaacag tttgtttaac acttccaggg agtctttctc ccagacaccc
     9181 atcgtggagt ggaaattaat atccttccga taatagagtt tttccccttt gatggcgccg
     9241 aaagggatct tatagaaatg gtcttcttta aaaaataagg caagaatact cctggccagg
     9301 gatgaattga agtggaaata gagtagtttt ctgatattca ttagatccag gttttccatt
     9361 ttgcatgtcc gccttcccac aattcattca gcattttttt atcgcggagg gtgtccatgc
     9421 atttccaaaa tccggaatgc cggtaattgt ataattgtcc ttccatagcc agcttctgca
     9481 aagggccctg ttcaaacacc gtatcatctc cggcaatata gtccagcact tcgggctggc
     9541 aggcaaaaaa accgccgttg atccaatttc catctcccct cggcttttcc agaaaagaat
     9601 ccacccggtc gccttccgtt tccaacgccc cgaaacggcc ctccggctga accgccgtca
     9661 tggtgatata tttcccgtgc ttctgatgaa agctgaccag ctcttttaga tttatgtccg
     9721 atacgccatc cccataagtc aggagaaatg gttccttccc gataaattcc tttgcccttt
     9781 taatacggcc acccgtcatc gtgtcctgtc cggtttccag cagggtcact ttccagggtt
     9841 cggaggtatt attgtgcacc tgcagttcgt tggtgctgag gtctatcgtg acatcgcttt
     9901 gatgcaaaaa ataattcgcg aaatattctt taatataata ccccttatat cccagcagca
     9961 caacgaactc ggtatatccg tagtgggaat atcctttcat tatatgccag agaatgggct
    10021 tgcctccgat ctctgccatc ggttttggtc tgatatcggt ctcttcgctc aggcgtgtac
    10081 caaaaccgcc ggccagtaaa agcactttca taaattcatt gtattaatat tggttcttta
    10141 agatcgggca aatccggacg ggcttcattg gtactatgaa tctttgcaaa tctcgttttc
    10201 aatttcaaaa aataagattc gaaataatga taagaaagat aagccacaaa aatcgttatt
    10261 gcatatacca ggccgtaaac ggtgatatag gggagccagc ggcccgggat cgccgggata
    10321 tatggcttta aaaacctgga caaaagccag attatcagca tatgatatac ataaagaccg
    10381 taagatatcc tgcccaggaa attcagcaat ttattttcca ggttcagcag cgaccgggaa
    10441 ttggtgctca cattcacaat aattatggca tagacaacag catgcagctc gggatcgaat
    10501 aaggaagaaa aatgcaccgg cttgtaaaaa atggaaaccg ccagaaaaga ccaggcgatc
    10561 acctgcagca gcggggtata aaataatttt aatatggaat gcttacggaa cacatagttc
    10621 gccattattc ctccgattgc catacagtca aaagaggtaa ggtaaatcac ggaataaatg
    10681 ggaccgttct caaaaatccg gaacccgatc ttgatagcca gataaatgac gataattccc
    10741 aataaggcgc ggaaaacatt tcccgcccgg ttaagcaaca agggccagaa taaataaaat
    10801 tgctcctcaa cgccaataga ccagagcggc gtaatgggca gaatgccgga ataagccacg
    10861 ttggacaata gaaaaatgta aaaaaataaa ttcaggccga gatgcttttc cgggactata
    10921 aggaatttaa acagaatgag cagaataaca aacccgaccg ccaggtaata aatcggccag
    10981 atcctcaaaa tccttctttt atagaacttc agcagatcga ccttttcata ttttttcttt
    11041 tccgtcaaca aaagataggt gatcaggtag ccgctcagta caaagaaaag cgttaccccg
    11101 aaaccggcca tcccggtatt atgataaccg aacgatttca atccaaatag ccggaggaac
    11161 tgatcgatat gaaaagtaat cacaataaat gccgccattg cccggatccc atttaaacct
    11221 ttgaaatata tcctgttcat tctttcttcg ctgccgggga tcatttttaa acgacctcca
    11281 cgtacgccat ctttttcaat tttgggttcc ttttaaaata cggggcttca aataatctga
    11341 atgacaaaaa ggcaatgggg atggccacga ccagcgaaat caaaagggaa agataaaacg
    11401 ttttcaggtt cattcctccc cacgcaataa gccagttgga gaccaggtgc gcgacaaacc
    11461 aatgccataa ataaaagctg taggagatct tgcccagaaa taaaaaaggc ctgctggata
    11521 agacccgggt aaaaatacct cccgaagtga tcaggcttat taacatccat ccgaacccaa
    11581 atgtcataat gggttcggca aaagccctga aaataaaagc ctttgttccg aagtgccgga
    11641 caaattccgt cgttataatg atttttcccg cgtaagaaat gagcagcccc agaataaagc
    11701 cggaagatcc aatggcccac cgcggcaata cccgtttcaa aacgtatagc tgggcggcaa
    11761 taatgccaaa cccgaattcg ataaaccggt accagatggc cgtgctggtg atggtctcct
    11821 tttccaggat atcattcgta tggttataga acaggatcag ccgaaaaata aggcagacgg
    11881 ccatcaatgc gaaaacccgc aaactcagtt ctctctgcga tctcagccgg aggaacaaga
    11941 aagggaaaag caggtaaaag tgaaattccg ttgccaggga ccagtaatgg ggggcgaaat
    12001 catactgatc caaaaaaata ttgatgaaga gcaggtgata actcatctgt ttcagcggaa
    12061 agatgcccgt atggtacagc aataagaacg aatcaaaaac tacgatcata taaaaagccg
    12121 gggcaatcct taaccatctt tttttcaaaa agccaaaaaa attggacgcg ctgaaatgat
    12181 cgatcctaga agcgtacatc agatacatac agaatccgct gatgacaaaa aacagatcaa
    12241 ccccgtgccg tccgaaggcc attccccggt taaggtcgag gccgcctact ttcatcggga
    12301 tatttttaaa aaacatccag acatgcgtcc acaaaacgcc cagtaccgca agggttctca
    12361 gcccgtcgat ggccgggatc cggctattct gtgttttcat gatggttttc gcgtctgccg
    12421 gatcagttct tcgattctgt ccagctgccg cttataggtg ttattcctgg caaagttgac
    12481 ccggtaatga aaaagctccg gagaattatg ggtatccagg ttacggaccg tatccaggaa
    12541 aagcgtgccc aaatttttgt tgttgttccg ctcggcaacc attctgacga gcgcgggctc
    12601 acccgcataa gccgtaatat tattggagac gatcaccttt ccggtactta tatactccat
    12661 caccttatgg taatttgttc ccctgctctg atccttctcg atatcataac agatcagcag
    12721 aacctgcatt tcattcagtg cggcggccag tgccgcggaa tctaaaacac catgcaattt
    12781 tacattagcg aggattttca gatctttaat aaaaccggcc gtccgattgt ccatacctgc
    12841 tccgatattg gattgcccga tttcataact cccgaaaaaa ttaaaatcca gttcctgatt
    12901 atcccggata atgcttaaca gggtgtcccg gtcaatatcc cttctcagca aattgccgga
    12961 aaacccgacc tgtatccggt ctccattacg gtggtccggc tgttctttga tgaattcatc
    13021 cgagatccca tggttaatca aatgccggga caaaggatat ttataccggg caaggatctc
    13081 cccggtgacc gaaaaaagca cgtcggcgcc ggcggaagcc cggattgcct ccggagtcct
    13141 gggttcatcc agcggatgga ataccttgta cacagacgga ttgaaatagc gtagcgggaa
    13201 caggtttccc agatcaaaag accagatcac gtcaacgggc ttgccgataa cccgcaaaat
    13261 gtccgcgatt tgtttccgga taaagaagtt atacaacctc ctggcgtgaa atattatttt
    13321 atagggaaaa aataattgct ggtcgatcaa aaaaaggttt gaatgatctg cggatttcaa
    13381 cacgctaatt cgctgcgtgg cccttttcca gctccagtga tcattatccg gcgggttcag
    13441 aaaataaacc tcatttcctc tactggccag ttcaatcgca taatggtgct tggcaagaag
    13501 catttttccc caggattggg gagaaaggat cagtattact ttatttgaaa ggaacccggc
    13561 atccattgat tttatcggtt tagcgtaagg atgcaataaa agacctgacc ccgtcggcaa
    13621 cataggcaat catttcctct gtcagttccg gaaacatggg taaagaaaga atttcattct
    13681 gtaaccgggt ggccaccggg aagtcggacg gcttataacc caaatattga tagaccacca
    13741 gattaggtaa ggccaccgga taatgcaccg ctgtttcaat tccctttccg gataaatagg
    13801 ccgccagttt ttcccgctgg ccgctgcgga tcacgtagag atgccaggta tgtttggtgt
    13861 ccgggcgctg cctgggaaga attattccgg gaatatcggc cagccattta tcataaagcg
    13921 cggcagattg aatcctcttt tcggtccagt ccccaaggtg gggcagtttt gcgctgagaa
    13981 tggcagcctg caaaccgtct aggcggctgt taatcccctc catttggtgc tgatgttttt
    14041 tcaaggcccc gtgatgggaa tacatcctgc atttatccgc aagggc
//
LOCUS       DASYCU010000630         7649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4340748, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000630 DASYCU010000000
VERSION     DASYCU010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4340748"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /gene="gatA"
                     /locus_tag="VGM24_12675"
     CDS             <1..1342
                     /gene="gatA"
                     /locus_tag="VGM24_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY4156284.1"
                     /translation="AFIQVYAEEAISTASRLDKERKSGKPPGKLHGVIIGIKDVLCYK
                     DHPVSASSRILQGFNPLYHATAVERLLNEGAILLGHLNCDEFAMGSSNENSIYGRARN
                     ALDPGLVAGGSSGGSAVAVQAGLCMAALGSDTGGSVRQPADFCGVIGLKPTYGRVSRY
                     GLIAYASSFDQIGIFGKNIDDTALLLQVISGADSRDSTCSARPVPEYREALDKPVTGF
                     RIAILKEALRHPSLDPEIRDQLTGFIDDLRSRGFQVEEIPFDLLDYIVPAYYILTTAE
                     ASSNLSRFDGIRFGRKTSDPEAPYPDFYRKTRSEGFGKEVKRRILLGTFVLSSGYYDA
                     YFTRAQQIRKILSTGTKLIFKQFDLILTPTVPTTAFRAGEKISDPVAMYLADLYTVYA
                     NLVGIPGISLPLFRHSNGMPFGVQAMTNQFQELSLLQFSSYLMQQYKSYEIPTL"
     gene            1448..2512
                     /locus_tag="VGM24_12680"
     CDS             1448..2512
                     /locus_tag="VGM24_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY4156285.1"
                     /translation="MKRLLLSGIAVIGFVFITQANALKRDKTSVSLYLPIDTSDVKKG
                     FDNLFLASVSGSNGARLNPRALSYVQDFILKNKKDLEELKSWGQPYLNLIDHILTQYS
                     LPSELKYLAVIESELKPTAVSWAGAVGPWQLMPATARELGLQVNRRVDERKNYRKSTR
                     AAALYLRDLYSEFGDWLLVIAAYNGGSANVCRAIKKSHSRNFWDLQYYLPAESRNHVK
                     KFIGTQYTFEGQGSVTTLTRKEAADQLSGSSMYVFKRNISKEEAANTKTVTVSGKYFS
                     TVISRHVMMDLDAFNRYNPDFDKIMASENNSYDLKLPADKMDLFISDKYQILNDSVQQ
                     LLLEDNDESNSKSLAGIASK"
     gene            complement(2552..3853)
                     /locus_tag="VGM24_12685"
     CDS             complement(2552..3853)
                     /locus_tag="VGM24_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEY4156286.1"
                     /translation="MSTRAFVSFPVSDLNKLKQQVLNWSAQFNTCCFLDNQQYKSPTH
                     SFECVVAAGEISRLETGAGTAFEQLRLFSSKQNDWLFGHFSFDLKSETESVPSGHPDQ
                     IGFPDLFFFIPEIVVLLNHDRLSIGLMNGNHDQVLNAIQNEPVNGTRERQKPVTLRQR
                     IQKEEYLDLIEKLKGHILFGDCYEINFCQEFYAEQADIDPIPLFKALTRVSPSPFSAC
                     YTVDGKYLLCASPERYLKRKEDLILSQPIKGTAPRDLQNKMNDEKLKQDLFTSEKDRT
                     ENVMIVDLVRNDLSRICKPDTVKVEELFGIYSFPQVHQMISTVQGSLKAGLDWTEMIA
                     ATFPMGSMTGAPKKRVLELIEQYEKTKRGLFSGAVGYVNPNQDFDFNVVIRSLLYNRH
                     NRYLACFAGSGITIGSDPEKEYEECIVKMAAIEELLKNPAG"
     gene            complement(3868..4326)
                     /locus_tag="VGM24_12690"
     CDS             complement(3868..4326)
                     /locus_tag="VGM24_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156287.1"
                     /translation="MATKSDKEKKDSKKSAPAPSKPTTAKPSSKKAKEEEEEDDDDDE
                     VETPKTNGKKGAKTPASKSKKKDDDEDDDDDGEEEDEVDEWDKVEEDEEWDPDFDEFD
                     LPRSRAKKGPVTGGKKPAKGGDEDDLGLDDDYKNLDLFNEGGFDEEEDDF"
     gene            complement(4416..5681)
                     /locus_tag="VGM24_12695"
     CDS             complement(4416..5681)
                     /locus_tag="VGM24_12695"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY4156288.1"
                     /translation="MVDVILGLQWGDEGKGKIVDYFAGRYDIIARFQGGPNAGHTLYV
                     NDQKIVLHQIPSGIFHENTINLIGNGVVLDPITLKRECESVKSFGVDYRKNLYIAQRA
                     HIILPTHRALDKASEFSKGNEKIGSTLKGIGPAYMDKTGRNGLRVGDLQDKNFTTQYI
                     RLRLKHQKLLDGLHFQEDVSAWEEEFFEALDFMRQFKIVPGEYFINDKIGQGKKVLAE
                     GAQGSMLDVDFGTFPFVTSSNTISAGVCTGLGVAPQQIRDVIGVTKAYCTRVGSGPFP
                     TELMDETGDRLRKAGSEFGATTGRPRRCGWIDLVALRYACMINGVTRLVMTKADVLDD
                     FEKLEVCTRYRIDGRETAEVPFQMLQQVIEPVLSSYTGWKSDITAINTYDGLPAAMKE
                     YISFIDSYLKVKVSYISNGPGRNQLIENT"
     gene            complement(5760..>7649)
                     /locus_tag="VGM24_12700"
     CDS             complement(5760..>7649)
                     /locus_tag="VGM24_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEY4156289.1"
                     /translation="RIVDGLTKISNVIDVNASQQAENFKKILLTLTDDPRVILIKLAD
                     RLHNMRTLDSMKRDKQLKISSETVYVFAPLAHRMGLYNIKTELEDLAMKYLEPEEYKE
                     IARKLAETKRERSRYINEFIKPLRENMERSNFDFEIYGRPKSIHSIWTKMRKKGVVFE
                     EVYDLFAIRIIINSPPEREKADCWKVYSMITDEYSPSPERLRDWLSNPKSNGYEALHT
                     TVMGPQGKWVEIQIRSKRMNEIAEKGLAAHWKYKEGASDESRFDKWFQQIREVLNLED
                     NDSINFLQDFKMSFLAEEIYVYTPKGDVKMMPIGSTALDFAFSIHSAIGEQCIGAKVN
                     HKLVPISHKLRSGDQVEIITSSKQHPTEDWLSFAVTAKAKSKIKDSLKDEKRKIADEG
                     KYIVQKKLENFGAAYNQHNIDTLTAFYKLGSSLDLYYQAAIKNLDLKELKEFQLLGDR
                     IEPPKPAKPVVEIKPEPGLTPAQRKDTELIIFGESSDKIVYTLANCCKPIPGDDVFGF
                     VTTGKGLTIHRTNCPNATRLLSSYGHRVVKTKWAKNKEISFLTGIRIVGIDDVGVINK
                     ITNLISGELRININALTVEAKEGLFEGSIKVYVHDKEELDEAVGKLKQLSGIHSVDRF
                     DTENG"
BASE COUNT         1786 a         1976 c         1791 g         2096 t
ORIGIN      
        1 cgcctttatc caggtttacg cggaagaagc gatttccacc gcgagccgcc tggacaaaga
       61 acggaaatcc ggaaagcccc ccggcaaact gcatggcgtg atcataggga taaaagacgt
      121 gctctgttat aaggatcatc ccgtatcggc ttcttcccgc atcctgcagg gatttaaccc
      181 gctctatcat gccaccgcag tggaacgcct gctgaatgaa ggcgcgattt tgctggggca
      241 cctgaactgc gatgaatttg ccatgggctc cagtaatgaa aattccattt atggcagggc
      301 ccggaacgcc ctggatcccg ggctcgttgc cgggggctcc tcgggaggct ctgcggtggc
      361 cgtacaggcc ggtctttgca tggccgccct gggctcggat accggcggct ccgtgcgtca
      421 gccggctgat ttttgcggcg taataggcct taaaccgacc tatggacggg tttcccgtta
      481 cgggctgatt gcctatgcct cgtcttttga ccagatcggc attttcggga aaaatataga
      541 cgataccgcc cttctcctgc aagtgatttc cggcgctgac agccgggaca gcacctgttc
      601 ggcccggccc gttccggaat accgggaagc cctggacaag ccggtcaccg gattccggat
      661 tgctatctta aaagaggccc tccgccaccc ttccctggac ccggagatcc gggaccagct
      721 caccggattt atagatgatc ttcgttccag gggctttcag gtggaagaaa tcccgtttga
      781 tctcctggat tatatcgttc ccgcttatta tatcctgacc accgccgagg cttcctcgaa
      841 tttatcccgg tttgacggca tccggtttgg ccggaaaacc agcgatccgg aagctcctta
      901 tccggatttt tatcgcaaaa ccaggtcgga aggctttgga aaagaggtaa aaagacggat
      961 cctgctggga acctttgttt tgagctccgg ttattatgat gcctatttta cccgggcaca
     1021 acaaattaga aaaattttat caacaggaac aaagctgatc ttcaagcagt tcgacctaat
     1081 tctcactcct accgtcccaa ccaccgcctt tcgcgccgga gagaagattt ctgatcccgt
     1141 cgccatgtat ctcgctgatt tatacacagt ttatgcaaat ctggtgggca ttccgggcat
     1201 ttctttaccc cttttccggc attcaaatgg catgcctttt ggtgttcagg ctatgacaaa
     1261 ccagtttcag gaattatctt tgcttcagtt ctcctcctac ctgatgcaac aatataagtc
     1321 ctacgaaatt ccgaccttat gagaccggat tcccgagcta ctatttccgg caaatacaat
     1381 ccgtaatcca tccagtgaaa aatttataaa ccggatttcc cggtctataa atctttaagc
     1441 atttgagatg aaaagacttc tattgtcggg cattgccgtg attggttttg tgttcattac
     1501 gcaggcaaac gccttaaaga gggacaaaac ttccgtttca ctttatttac cgatcgacac
     1561 cagcgatgtc aaaaaaggct ttgacaacct gttcctggct tcagtcagtg gcagcaacgg
     1621 agcccgatta aatccccggg ctctcagcta tgtccaggat ttcatcctga agaataagaa
     1681 agacctggag gaattaaaat cctggggtca gccatattta aacctgatcg accatattct
     1741 tacccagtac agccttcctt ccgaactcaa atacctggct gttatagaat ccgagctgaa
     1801 accgacggct gtttcctggg ctggtgcggt gggtccctgg cagctgatgc cggctaccgc
     1861 ccgtgaactg ggtctccagg tgaaccgccg ggtggatgaa cggaagaact accggaagag
     1921 cacccgggcc gccgctcttt acctgcgcga cctctattca gaatttggcg actggctgct
     1981 ggtgatagcg gcctataacg gaggttcggc caatgtatgc cgggccatta aaaaaagcca
     2041 tagccgtaat ttctgggatc ttcaatatta cctgccggca gaatccagga accacgtgaa
     2101 aaaattcatc ggaacccagt atacttttga agggcaggga agtgtgacca cccttacccg
     2161 gaaagaagcc gccgaccagc tctccggttc ctccatgtat gtgttcaaaa gaaacatcag
     2221 caaggaagag gcagcgaata caaaaaccgt gaccgtttca ggtaaatatt tttcaacggt
     2281 tatctccaga catgtaatga tggatttgga tgcgttcaac cggtataacc cggattttga
     2341 taaaataatg gccagtgaga ataacagtta cgatctgaaa ctgcctgccg acaagatgga
     2401 tctttttatc tccgataaat accagatcct gaatgattcc gtgcagcagc tgctgctgga
     2461 agataatgac gaaagcaaca gcaagtccct ggcgggaatt gccagcaaat agcctctttt
     2521 tgcatttgaa aatcacgaac cctgcccgaa ttcatccggc agggtttttt aataactctt
     2581 caatggcggc cattttcacg atgcattctt catactcttt ttccggatca ctgccgatgg
     2641 taattccgga accggcaaaa catgccagat agcggttgtg ccggttgtac agcaggcttc
     2701 tgatgaccac attaaaatca aaatcctggt tggggtttac atagcctacg gctcccgaaa
     2761 ataatccgcg ctttgtcttt tcatattgtt caatcaattc cagcacccgt ttcttgggtg
     2821 ctcccgtcat ggatcccatg gggaaggtag cggcgatcat ttccgtccag tccaacccgg
     2881 cttttaaact tccctgaacg gtagagatca tctgatggac ctgcggaaag gaataaattc
     2941 cgaacagctc ttccactttc acggtgtcgg gtttgcagat ccgggaaaga tcgttgcgga
     3001 ccagatccac gatcatcaca ttttcggtac ggtccttctc actggtgaac aaatcctgtt
     3061 tcagcttttc atcgttcatt ttattttgca ggtcccgggg agccgttcct ttgatgggct
     3121 gggacagtat cagatcctcc tttcttttca gatatctttc gggactggcg cagagcaggt
     3181 atttcccgtc caccgtgtaa caggcagaaa acggggaagg ggaaacccgg gtcagggcct
     3241 taaacaaagg gatgggatcg atatcagcct gctccgcata aaactcctgg caaaaattaa
     3301 tttcataaca atcgccaaat aaaatatgcc ctttcagttt ctcaatgaga tcaaggtact
     3361 cttctttctg tatccgttgc cggagggtca ccggcttctg cctttcccgt gtcccgttca
     3421 cgggttcgtt ttgaatggca ttcaacacct gatcgtgatt gccattcatt aatccgatgc
     3481 ttaaccggtc atgattcagc aggacaacaa tctccggaat aaaaaagaaa agatccggga
     3541 agccgatctg atccggatgc cccgagggaa cggattcggt ttctgatttc aggtcaaagg
     3601 aaaaatgacc gaagagccag tcgttttgtt ttgaggagaa taaccggagt tgttcaaagg
     3661 cagtcccggc gccggtttcc agccgggaaa tttctcccgc cgcaacaacg cattcaaaag
     3721 aatgcgtggg ggatttgtat tgctgattgt ctaaaaagca acaggtatta aattgggctg
     3781 accagtttaa tacctgttgc tttaatttat tcagatcgct tacgggaaaa gaaacaaaag
     3841 cgcgggtaga catttcattc ctccggatta gaaatcatct tcttcctcat caaaaccgcc
     3901 ctcgttaaag aggtccaggt ttttataatc atcatccagg cccagatcat cttcatcgcc
     3961 tccttttgcc ggttttttcc ctccggtaac aggacctttc ttggcgcggg agcggggaag
     4021 atcgaattca tcaaaatcgg gatcccactc ctcatcctct tccactttat cccattcatc
     4081 cacttcatcc tcttcctcgc catcgtcgtc atcgtcctcg tcgtcgtctt tcttttttga
     4141 tttggaggct ggagtttttg cacccttttt cccgtttgtt ttaggagttt ccacctcatc
     4201 atcatcgtca tcctcttcct cttcttcttt ggcttttttt gaagaaggtt tcgcagttgt
     4261 tggctttgat ggtgcaggtg cggatttttt tgaatccttt ttctccttat cagattttgt
     4321 tgccatgttc caataagatt aggttgtaaa atttcagcga agtttttaat ccgccaaatt
     4381 tttttaaaaa agattttttt aatccccttg acggattaag tattttcaat caattggtta
     4441 cgaccggggc catttgatat atatgaaacc ttaactttca ggtaactatc tataaaagaa
     4501 atatactctt tcatcgcggc cggcagaccg tcgtaggtgt ttattgctgt aatatccgat
     4561 ttccagcccg tataggagga gagaacgggt tcaataacct gctgcaacat ctggaaggga
     4621 acttcggcgg tttccctgcc gtcaatccgg taccgggtac aaacttccag tttttcaaaa
     4681 tcatccagca catccgcctt ggtcatcacc aggcgggtca ctccgttgat catgcaggca
     4741 taccgtagcg ccaccaggtc aatccagcca cagcgcctcg gtctgccggt ggtggcgccg
     4801 aattcgcttc ccgctttacg caggcggtct cctgtttcat ccatcagctc ggtggggaaa
     4861 ggaccgctcc ccacccgggt gcaataagct ttggttaccc cgatcacgtc ccggatctgc
     4921 tgcggggcta ctcccagtcc cgtgcagaca cccgcagaaa tggtattgga ggaagtgaca
     4981 aacgggaagg ttccgaaatc cacatccagc atactgccct gcgctccttc tgcaagaacc
     5041 tttttaccct ggccgatctt gtcgttgata aaatattctc ccggaacaat tttaaactgg
     5101 cgcataaaat ccagggcttc gaaaaactct tcttcccagg cggaaacatc ttcctgaaag
     5161 tgaagaccat ccagtaattt ctggtgcttc aaccggagcc gtatatactg ggtggtgaaa
     5221 ttcttgtcct gcaggtcgcc cacccgtaaa ccgttccggc cggttttgtc catatatgcc
     5281 gggccaattc cctttaaggt ggatccgatc ttttcattcc cttttgaaaa ctcggatgct
     5341 ttatccagcg cgcgatgggt gggcagaatg atatgcgccc gttgggcgat gtacagattc
     5401 ttacggtagt caacgccaaa tgatttgacc gactcacatt cccgcttgag ggtaatcgga
     5461 tccagcacca cgccgttccc gatcaggttg attgtatttt cgtgaaagat cccggatgga
     5521 atctggtgaa gaacgatctt ctggtcattg acatataggg tatgtccggc atttggccct
     5581 ccctggaaac gggcgataat atcatacctg cccgcgaaat agtccacaat cttgccttta
     5641 ccctcatccc cccattgcaa gcctaaaata acatcaacca tcagtagtga tttgcgattt
     5701 aacggaaagc cgcaaaaata agcgaatgga acccaatgtt aaattatttt catccgccct
     5761 cagccgtttt cggtatcaaa ccggtccacg gaatggattc cgctaagctg cttcagcttg
     5821 ccgacggctt catccagttc ttccttatca tgaacataga ctttgatgct cccttcaaac
     5881 aggccttctt tcgcctccac ggtgagggca ttgatattga tgcgcagttc accggatatc
     5941 aggttggtga tcttattgat cacccccaca tcatcgattc ccacaattcg gataccggtg
     6001 agaaaggaga tctctttgtt cttggcccat tttgttttaa ccacgcggtg accatagctc
     6061 gacaggagac gggtggcatt cgggcaattg gtgcgatgga tggttaatcc tttcccggtg
     6121 gttacaaacc cgaatacatc gtctccgggt atgggcttgc agcagttggc cagggtgtac
     6181 acaatcttgt cactgctctc tccgaaaata atgagttcgg tatccttgcg ctgggcgggc
     6241 gtaagacccg gctcgggctt tatttcgacc acgggtttgg ccggcttcgg cggttcgatc
     6301 cggtctccca gcagctggaa ttccttcagc tccttcagat ccagattctt gatcgccgcc
     6361 tgatagtaaa gatccaggga cgagccgagc ttataaaaag cggtcagcgt gtctatatta
     6421 tgctgattgt atgccgcacc gaaattttcg agtttttttt gtacgatata ttttccttca
     6481 tcggcgatct tccgcttttc atccttgagg gaatccttta tcttactttt agctttggcc
     6541 gttaccgcaa agctgagcca gtcttctgta ggatgctgtt tgctggaagt aatgatctcc
     6601 acctggtcgc cgcttctcag cttatgactg atgggcacca gtttgtggtt cactttggca
     6661 cctatacact gttccccgat agcactatgg atggagaagg caaaatcaag ggcggtcgat
     6721 ccgatgggca tcattttcac atcgcccttg ggcgtgtata cataaatttc ctccgccaga
     6781 aaactcattt tgaaatcctg caggaagttg atggaatcat tgtcttccag attcagcact
     6841 tcgcggatct gctggaacca tttatcgaag cggctttcat cgcttgcccc ctccttgtat
     6901 ttccagtggg cggcaagacc tttctccgca atctcattca tccttttgga acggatctgg
     6961 atctccaccc attttccctg ggggcccatg accgtggtgt gcagggcttc atatccattg
     7021 gatttgggat tgctaagcca gtcgcggagt ctttccggcg aaggcgaata ttcatccgtg
     7081 atcatggaat agaccttcca gcaatcggct ttttcccttt cgggcgggga attaataata
     7141 atacggatcg caaagaggtc gtaaacttct tcaaagacca cgcccttttt tcgcattttg
     7201 gtccagatgg aatgaatgct tttgggccgg ccgtagatct cgaaatcaaa atttgaccgc
     7261 tccatattct cgcgcagcgg tttgatgaat tcattgatat accgggaccg ctctcttttg
     7321 gtttctgcca gcttgcgggc gatctcctta tattcttccg gctcgaggta tttcatcgcc
     7381 aggtcctcca gctcggtttt gatattgtac aggcccatgc gatgcgccag gggggcaaat
     7441 acataaacgg tttccgaaga gatcttgagt tgcttgtcgc gcttcatgct gtccagcgtg
     7501 cgcatattat gcaggcggtc agccagtttg atcaggatca cccggggatc atcggtaagg
     7561 gtgagcagga ttttcttgaa attctccgcc tgctgggagg cattcacatc aattacattg
     7621 gagatcttgg tgagcccgtc cacgatccg
//
LOCUS       DASYCU010000631         7662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_718608, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000631 DASYCU010000000
VERSION     DASYCU010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718608"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGM24_12705"
     CDS             <1..366
                     /locus_tag="VGM24_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY4156290.1"
                     /translation="ATGWKDEKLMVEKINDVLDKVGLKSKGFKMAYEMSGGEQQRVDI
                     ARALLNSPKLILADEPTGNLDPETADEIMQLLFQISKEYGTTVIMATHDYRVVHKFPA
                     RTIRTEKGKVFDNATISAL"
     gene            complement(347..1477)
                     /locus_tag="VGM24_12710"
     CDS             complement(347..1477)
                     /locus_tag="VGM24_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY4156291.1"
                     /translation="MQVQGKKLHIVCHDVPYPPDYGGVFDLFYKIRTLHEEGIRILLH
                     CFEYGRGEQPVLNEYCEKVYYYPRRTGHKGFSYHLPYIVCSRSNPELLDRLLEDDFPI
                     LLEGVHCTYLMNDARFAGRKVFLRLHNVESVYYKQLAGCTSSWFKKLYYLHESSVLRK
                     YEQRIASRWPLLAVSKTDAEAARSAYHAKEVLVIPVFLPYQQIDSPQGTGCYCLYHGN
                     LSVEENEKAVCWLLENVFDDLPLPFLIAGKKPSSHLRRTAARYRHACIIADPTGPELQ
                     DIIAKAQLHVIPSFNCTGIKLKLLNALYNGRHCVVNAEAVEGTGLKEVCHVAQEAGQF
                     KEVIETLYERPFTLLDLQFRQEKLLKQFNNQVSARRLIERIW"
     gene            complement(1479..2711)
                     /locus_tag="VGM24_12715"
     CDS             complement(1479..2711)
                     /locus_tag="VGM24_12715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY4156292.1"
                     /translation="MTFSPENPGENPNGFMTNSSHPTQGKLIIATVISDLATDYRVHR
                     ICQTLREAGYRVLLTGSVRRNSLRLEKRDYQTDRLKLWFNRTALFYAEFNIRLFFRLL
                     FKRGDIYLGNDLDVMPATRLLAWLHKKPLVYDSHEYFLGMAGMDQKPFRRGIWKFIER
                     RLFRRLKYAYTVCDSIQALYRQDYDKTLSVVRNLPLLEISPGASGSGEKDRIHSIDRQ
                     IPPGKWLLLLQGAGINTQRGAEELVLSMLFLDEARFHLLIIGGGDIFPDLQKIVAQNK
                     LQSKVSLVSRVPFALLNHFTRQAHLGLSIDKPSVINHRYSLPNKLFEYLHAGVPVLAS
                     RLVEQEKIINHYQVGTFIENHQPEHIAQKIREIFENQDQLNYWKENTRLVRKELNWEN
                     ESKIIIDIFKQVETDSVT"
     gene            2785..3057
                     /gene="rpsO"
                     /locus_tag="VGM24_12720"
     CDS             2785..3057
                     /gene="rpsO"
                     /locus_tag="VGM24_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY4156293.1"
                     /translation="MPYLTKEKTTEFFTKFGTSATNTGSIEAQVALLTERINRISEHL
                     QANKKDFSTHRGLMQLVGKRKRLLTYLSNTNLQSYRQLIEKIGLRK"
     gene            3194..5404
                     /gene="pnp"
                     /locus_tag="VGM24_12725"
     CDS             3194..5404
                     /gene="pnp"
                     /locus_tag="VGM24_12725"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY4156294.1"
                     /translation="MLSQPISVTFTTGEGQPITIETGKLARQADGAVTVRQGNCIILA
                     TVVANKDPREGQDFFPLSVDYQEKFASAGRIPGSFFKREARLNDYEVLTSRLIDRALR
                     PLFPDDYLCDVQVLVSLVSSDPEIMPDALAGLAASSALAVSDIPIKEVISEVRVAKLN
                     GKFIVNPTRTELETADLEFLIAATEKNLLMVEGEANECSEEDLIQALQVAHDAIRIQI
                     AGQEELRRKAGSKTKRDYKKPEQNEELQAKVNAFAKDKVYQISRAGSSKHERSEAYDA
                     VKKELIESLGEEVSDTDKKLAKKYYADLQWEVVRNMILDESVRLDGRKLDQVRPLAME
                     TDPLPTPHGSALFTRGETQSLTTVTLGTPLDELLVESAAKSDYTKFILHYNFPPFSTG
                     EVKMMRAPARREVGHGNLAMRSLKKMMPGKDYPYTVRVVSDVLESNGSSSMATVCAGS
                     LALMDAGVPFPKHVGGVAMGLITRPSDNKHAVLTDILGDEDHLGDMDFKVTGTREGIC
                     GVQMDIKVDGLSMETMREALEQARKGRLHILEAMYTCMPAAREEVKPHAPRMEKIFID
                     KEFIGAVIGPQGKIIQEIQRETGATINLEEVGNYGEVSIFSPAKEGLDKAVAWIKGIV
                     AVPEVGTVYEAKVKSVMPYGAFVEFLPGKQGLLHISEVSWKRLESMDGVLNENDMVKV
                     KLIGVDNKTGKFKLSRKALMPKPEGRPEGRPEGRPEGRPEIRPQAERQESGKKE"
     gene            5443..6285
                     /gene="prmA"
                     /locus_tag="VGM24_12730"
     CDS             5443..6285
                     /gene="prmA"
                     /locus_tag="VGM24_12730"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY4156295.1"
                     /translation="MNAYVEVSLPVKSDLESDLMVAQLSRIGFEGFEEEEGRLLAFIP
                     QTFYDESQLLRTVLTSKGLERGDPDQLYSIRVIEEKNWNEEWEKDFHPVIIDDFCAIR
                     AHFHEPVTTVKHELIITPKMSFGTGHHASTYMMIEAMRGLDFYAEKVLDFGTGTGILA
                     ILAEKLGSVDIRAIDEDPRSIENARENMAANRTIHISLEQKDSLSGEAVFDIILANIS
                     LSVILRNLGPLWQHLDENGVILASGVLQTDEEEIRQNARKEGFLMNILFIRDNWMSFS
                     LKKR"
     gene            6356..7030
                     /gene="plsY"
                     /locus_tag="VGM24_12735"
     CDS             6356..7030
                     /gene="plsY"
                     /locus_tag="VGM24_12735"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY4156296.1"
                     /translation="MKEVLFIFIAYLIGSIPTAVWISRYFFGVDIREYGSGNAGATNT
                     FRVLGSKWGSIVMSVDVLKGVVATSLYIVLPFYMNNEWDRTNLMVGLGLAAVVGHIFP
                     LWADFRGGKGVATLFGMILAIQPIVAVYCVGVFLLCLYLTRFVSLSSILASIAFAVLI
                     LFIFNEKEPLYRAFAIAVALLVILTHQKNISRLLRGSESKIPILKYRDKRRHRRPDSA
                     IDDDKH"
     gene            7165..7599
                     /locus_tag="VGM24_12740"
     CDS             7165..7599
                     /locus_tag="VGM24_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156297.1"
                     /translation="MSQTLLDKLELKDEKSVLIQGLPSSIEKQFAKLAFAKNVTPLLK
                     IRKIDFALVFAVNENQLRGIILEVLPALSKGGKFWVAYPKTSSKIATTLNRECSWTCI
                     TQAGFEGINEITLDHVWTATRFEKASRQGILSTRSLTAMVDR"
BASE COUNT         2043 a         1796 c         1961 g         1862 t
ORIGIN      
        1 gccaccggct ggaaagatga aaaactgatg gttgaaaaga tcaacgatgt attggataag
       61 gtcggtttaa aatcgaaggg gtttaaaatg gcctatgaaa tgagtggcgg ggaacagcaa
      121 agggtggaca tcgccagggc cttactgaat tctcccaaac tgatcctggc cgacgaaccg
      181 accggaaacc tggatccgga aaccgctgat gaaatcatgc aattgttatt tcagatctcg
      241 aaagaatatg ggacaaccgt gattatggct acccatgatt accgggtggt tcataaattt
      301 cccgcgagaa caatccgcac ggaaaaaggc aaagtctttg acaatgctac catatccgct
      361 ctataagtct gcgcgcggaa acctggttgt tgaactgctt gagtaatttt tcctggcgga
      421 attgtaaatc cagcagggtg aacggcctct catacagggt ttcaatcact tccttgaact
      481 ggcctgcttc ctgcgccaca tggcatacct ctttcaggcc ggttccttcc accgcttctg
      541 cattcaccac gcaatgcctt ccgttgtaaa gggcattcag cagtttcaat ttgatcccgg
      601 tgcaattaaa agacgggatt acgtgcaatt gggcttttgc aatgatatcc tggagttctg
      661 gaccggtggg atctgcgatg atgcaggcgt ggcggtagcg ggccgccgta cgacgcaaat
      721 gggaggatgg ctttttgccg gcgatgagaa agggtaaggg gaggtcgtca aaaacatttt
      781 ccagcagcca gcataccgct ttttcatttt cttccacaga caaattgccg tgatataaac
      841 aataacaacc ggttccctgc ggggaatcga tctgctgata gggaagaaag accgggatga
      901 ccaaaacctc ttttgcgtga taagccgacc tggctgcttc ggcgtccgtt ttacttactg
      961 ccagcagggg ccagcgcgaa gcgattcttt gttcgtattt tctaagcacg gaactttcat
     1021 gcagatagta taatttctta aaccaggagg aggtacagcc ggcgagctgt ttataataaa
     1081 ccgattccac attgtgcaac cggaggaata ccttccggcc cgcgaaacgg gcatcattca
     1141 tcagatacgt gcagtggacc ccttccagga gaatggggaa atcgtcttcc agcaaccggt
     1201 ccagcaactc cggattggaa cgggaacaaa cgatataggg caggtggtag gaaaatccct
     1261 tatgtccggt gcggcggggg taataataga ctttctcaca gtattcattc agaaccggct
     1321 gctctcctct tccgtattca aaacaatgca ggaggatacg gattccttct tcatgaagtg
     1381 tcctgatttt ataaaataaa tcgaatacgc ctccgtaatc cggcggatac ggaacgtcat
     1441 ggcaaacaat atgtagtttt ttcccctgca cctgcatatt aagtaaccga atccgtttcc
     1501 acttgtttaa aaatgtcgat gattatttta ctttcattct cccagttcag ctccttccgg
     1561 accaaacggg tattttcttt ccagtaattc aattgatcct ggttttcaaa tatttccctg
     1621 attttttggg caatatgttc cggctggtga ttctcgataa aggttcctac ctggtagtga
     1681 ttgatgatct tttcctgttc aaccagccgg ctggcgagaa cgggaacgcc ggcatgcagg
     1741 tattcaaata atttgttggg cagactgtag cggtgattga tcacggacgg tttatcgatc
     1801 gacagcccga ggtgcgcctg gcgtgtgaaa tgattcaata aggcgaaagg aacccgggaa
     1861 accagactaa ccttgctttg aagtttgttt tgggccacta ttttctgcag gtccggaaaa
     1921 atatctcccc cgccgatgat cagcaggtga aagcgggctt cgtccagaaa aagcatggaa
     1981 agcacgagtt cttcggcgcc gcgctgggta tttattccgg ccccctgcaa gagaagcagc
     2041 catttgccgg gcggtatctg gcggtcaatg gaatggatcc ggtctttttc gccggaaccg
     2101 gatgctccgg ggctaatttc aagaaggggc aggttcctga ccacggaaag ggtcttatca
     2161 taatcctgcc ggtacagggc ctgaatggaa tcgcaaaccg tataggcata tttcagccgc
     2221 cggaagagcc tcctttcaat gaatttccag atcccgcgcc ggaagggttt ttggtccatt
     2281 ccggccattc cgaggaaata ctcgtggctg tcgtagacca ggggtttttt gtgcaaccag
     2341 gccagcaagc gggtggccgg cataacgtcc aggtcgttgc ccagatatat atcccctctt
     2401 ttaaaaagaa gccggaaaaa tagccggata ttgaactctg catagaacag ggcggtcctg
     2461 ttaaaccata gttttagccg atctgtctga taatcacgtt tttctaaccg gagactgttc
     2521 cggcggaccg aaccggtcaa aaggacccgg taaccggctt cccggagggt ctggcaaatc
     2581 cggtgcaccc ggtagtcggt cgcaaggtcg gaaatgaccg ttgcgatgat cagctttcct
     2641 tgtgttgggt ggctggaatt ggtcataaag ccgtttgggt tctccccggg attttcaggt
     2701 gaaaacgtca taattctcaa ataattctat accttcgcgc cgaaattagc agaatattta
     2761 tctggatgcc ttaaaaaaat tgagatgcct tatttgacta aagaaaaaac aacagaattc
     2821 ttcactaagt tcggaaccag tgcgacgaat accggatcca ttgaagcgca ggtggcctta
     2881 ctgaccgagc ggattaaccg catttccgag cacctgcagg ccaataaaaa agatttttcc
     2941 actcaccggg gactgatgca gctggttgga aagagaaaac gccttttaac ttacctgagt
     3001 aataccaacc tgcaaagcta ccgccagctg atcgaaaaga tcggtctaag aaaataaaac
     3061 atttaagtaa aaagtgaaat tcccaatacc tgactgttgg gaatcatttt ttagaggttc
     3121 acatctttta cgttaactat tcatggatcc cgcccttcac caaccagatc catcgatttc
     3181 aaaagcatca tttatgttat cacagcctat cagtgttacc tttactaccg gtgagggtca
     3241 gcctattact atagaaaccg gtaaactcgc ccgacaggcg gacggtgccg ttaccgtgcg
     3301 tcagggtaac tgcattatac tggccacggt agtggccaac aaggatcccc gggaaggcca
     3361 ggatttcttt ccgctttccg tcgactacca ggaaaaattt gcatctgccg gcaggattcc
     3421 cggttctttt ttcaaacggg aagcccggct caatgattat gaagtgctca cgagccggct
     3481 gattgaccgc gccctgcgtc cgctgtttcc cgatgattat ttatgcgatg tgcaggtatt
     3541 ggtcagcctg gtttccagcg atccggaaat tatgccggat gcgctggcag gacttgctgc
     3601 ttcttccgca ctggctgttt ccgacattcc catcaaggaa gtcatcagcg aagtacgggt
     3661 ggccaagcta aacggtaaat tcattgtgaa tccgacacgg acggaactgg aaacggccga
     3721 cctggaattc ctgatcgccg cgacagaaaa aaatctcctg atggtcgaag gagaagccaa
     3781 tgaatgcagc gaggaagacc tgatccaggc attgcaggtg gcgcatgatg ccattcgcat
     3841 ccagatcgcg ggccaggagg aacttcgccg gaaggccggt tcaaaaacga aacgggatta
     3901 taaaaaaccc gaacagaatg aagagctgca ggctaaagta aatgcctttg ccaaagataa
     3961 ggtctaccag atctcccgcg ccgggagcag caagcacgaa cgcagcgaag cctatgatgc
     4021 agtaaaaaaa gaattgatcg aaagtctcgg tgaagaagtc agcgataccg ataaaaagct
     4081 tgcaaaaaaa tattatgccg atcttcaatg ggaagtggtc cgtaatatga tactggacga
     4141 aagtgtccgg ctggacggac ggaagctgga tcaggtcaga ccgctggcta tggaaacgga
     4201 tccattgccg acaccgcacg gatcggcctt gtttacccgc ggagaaaccc aatctcttac
     4261 cacggtgacc ctgggcacgc ccctggatga attgctggtg gaaagcgcgg caaaatcaga
     4321 ctataccaaa tttatcttgc actataattt cccccccttt tccaccggtg aagtaaaaat
     4381 gatgcgggca ccggcaagac gcgaagtagg gcacggaaac ctggcgatgc gttccttaaa
     4441 gaaaatgatg cccggaaagg attatccgta tacggtccgt gtggtcagtg atgtactgga
     4501 atccaacggt tcttcctcca tggctacggt ttgtgccgga tctcttgcgc tgatggatgc
     4561 cggtgttcct tttccaaaac acgttggcgg tgttgcgatg ggattaatca cccggccttc
     4621 ggataacaaa catgcggtgc ttaccgatat tctcggagat gaagatcatc tcggggacat
     4681 ggacttcaag gtgaccggaa cccgcgaagg gatctgcggt gtgcagatgg atatcaaggt
     4741 cgacggactc agcatggaga ccatgcggga agcgctcgaa caggcacgga aaggaaggct
     4801 tcatatcctg gaggccatgt atacctgtat gcctgccgcc agggaagaag tgaaaccgca
     4861 cgccccccgg atggaaaaaa tatttatcga taaggaattt atcggagccg tgatcgggcc
     4921 gcagggaaag atcattcagg agattcagag agaaaccggt gcgaccataa acctggaaga
     4981 agtcgggaat tatggtgaag tgagcatatt cagtcctgcg aaagaaggcc tggataaggc
     5041 ggtggcctgg atcaaaggta tcgtcgccgt gcctgaagta ggcaccgtat acgaggcaaa
     5101 ggtaaaatcc gttatgccgt atggcgcatt tgtagaattc ctgccgggaa aacagggatt
     5161 gctgcatatc agtgaggtga gctggaaaag actggaaagc atggacggtg tattgaatga
     5221 aaatgacatg gtgaaagtta aattgatcgg cgtggataat aagaccggca aatttaagct
     5281 cagccggaaa gcgctcatgc caaaacccga aggcaggccg gaaggaagac ccgaaggcag
     5341 accggaagga agaccggaga tccggcccca ggcggagcgg caggaatccg ggaaaaaaga
     5401 ataagcacag cagtcgatag gccggccgaa taaaaacatt ccatgaacgc ctatgttgaa
     5461 gtctcccttc cggtaaaaag cgatctggaa tctgacctga tggtggctca attaagccgg
     5521 atcggtttcg aaggttttga agaagaggaa ggccggctgc tcgccttcat acctcaaacc
     5581 ttctatgatg aatcgcagtt gctgcgaacg gttctgacat caaagggatt ggagagagga
     5641 gatccagacc agttatattc tataagagta attgaagaga agaactggaa tgaagaatgg
     5701 gagaaagatt ttcatcccgt gatcatcgat gatttttgcg ccatccgggc gcactttcat
     5761 gaaccggtta caacggtaaa acatgaattg atcatcaccc ctaaaatgag ttttggtacg
     5821 ggtcatcatg ccagcacgta tatgatgatt gaagccatgc ggggcctgga tttctacgcc
     5881 gaaaaggttt tggatttcgg aacgggtacc ggcattctgg ccatactggc cgaaaaactc
     5941 ggatccgtcg atatccgtgc catcgacgag gacccccgga gtatcgagaa tgcgagggaa
     6001 aatatggcgg ccaaccggac aatccatata tccctggaac agaaggattc cttatccgga
     6061 gaggccgtct ttgacattat tctggccaat atcagcctgt ccgtcattct ccggaacctg
     6121 ggtcctttat ggcaacattt ggacgaaaat ggcgttatcc tagccagtgg cgtgctgcaa
     6181 acggatgaag aagaaatccg gcaaaacgcc aggaaagaag ggtttttaat gaatatttta
     6241 tttatccggg ataactggat gagtttcagt ttaaaaaaac ggtaactctc cggtaatttg
     6301 cggggaataa tctatttttt cgttattttt gaaatccatt tatccctaag ctttcatgaa
     6361 ggaagtttta ttcattttca ttgcctacct gattggctca attcccacgg ctgtttggat
     6421 aagccgttac tttttcgggg tagatatccg ggaatacggc agcgggaatg ccggagcaac
     6481 caataccttt cgtgttcttg gttcaaaatg gggatccata gtcatgtctg tcgacgtact
     6541 caagggagtg gtcgccacaa gcctttatat cgtccttccc ttttatatga acaatgaatg
     6601 ggaccgcacc aacctgatgg tcggactggg actggccgcc gtcgtcggac acatatttcc
     6661 gctctgggcg gatttccggg gagggaaggg ggttgccact ttattcggaa tgattctcgc
     6721 cattcagccg atcgtagcgg tatattgcgt gggtgtcttt cttttatgtc tttacctgac
     6781 ccgctttgta tccttaagct ccatattggc cagcatcgcc tttgccgtac tgatcttatt
     6841 tatttttaat gaaaaagagc ctttgtacag ggcgtttgcc atagccgtgg ccctgctcgt
     6901 catactaacc caccagaaaa acatcagccg cctgctgaga ggaagcgaaa gcaagattcc
     6961 cattttgaaa tatcgtgaca aaaggaggca ccgccggccc gattcagcga tagatgatga
     7021 caagcattag cgtagttcct gttaaatccc ctgaataacc gctttatttg agaattggta
     7081 agtgtttttc ttttacttcc gggtggattt ttctaccttt gccccccgct gaccgcgttt
     7141 ccggaaatta aaaactgttg aagcatgtca caaactttat tggataaact ggaacttaaa
     7201 gatgaaaaaa gtgtgttaat tcagggcctt ccttcctcca tagagaaaca gtttgccaag
     7261 ctggcttttg caaaaaatgt gaccccccta cttaaaatca gaaagataga tttcgccctg
     7321 gtttttgctg taaacgaaaa tcagctccgg ggaatcatcc tggaagtgct tcccgcgctc
     7381 agcaaaggcg gaaagttctg ggtagcttat cccaaaacga gctcaaaaat tgctaccacc
     7441 cttaaccgcg agtgcagctg gacctgcatc acccaggcgg gttttgaagg aatcaatgaa
     7501 ataacgctgg accatgtctg gacggccacc cgctttgaaa aagccagccg tcagggtatc
     7561 ctgagcaccc gcagccttac ggcgatggtt gaccgttgac cggtcattcc ccctgatccc
     7621 ggttcagact ttaacaatca ccgttcccga gttatccatc ag
//
LOCUS       DASYCU010000632         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1294941, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000632 DASYCU010000000
VERSION     DASYCU010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294941"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1095
                     /locus_tag="VGM24_12745"
     CDS             70..1095
                     /locus_tag="VGM24_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY4156298.1"
                     /translation="MGLFNFLTQEIAMDLGTANTLIIHNDEIVVNEPSIVALDRNNPK
                     TVLAVGKRALMMHEKTHESIRTVRPLKDGVIADFNAAELMIREMIKMIYPKKPLFPPS
                     WRMMICIPSSITEVEKRAVRDSAEQAGAKEVYLIHEPMAAALGIGIDVEEPVGNMIID
                     IGGGTTGITVIALAGIVCDQSIRIAGDEFTADIMEALRRYHSLLIGERTAEQIKIQIG
                     AALKDLDNPPDDIPVNGRDLVTGIPKQILVSYQEIAEALDKSIFKIEEAILKALETTP
                     PELAGDIYRRGLYLTGGGALLRGLDKRLSQKIKLPVHIADDPLKSVVRGTGISLKNYD
                     RYPFVMR"
     gene            1136..1990
                     /gene="mreC"
                     /locus_tag="VGM24_12750"
     CDS             1136..1990
                     /gene="mreC"
                     /locus_tag="VGM24_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY4156299.1"
                     /translation="MRNVFLFIRKYANFLFFLVLQIIALSFLFRYNKYHEAVFMNFAG
                     EITGSLDQRYSTVEYYFKLKKINEQLATENARLNQMLKENYERPDANGKLVMDTVQLD
                     SLRQVQKYTWLGARVVGSTVSTQTNFITIHRGSNQGVQPNMGVVSPLGIVGTVVNVSQ
                     NFASVMTILHRQYKVVVKLKNSGDRGTVEWDGISPSFVTLKDIPKSAKVQKGDSVVTS
                     PTSSLFPGNIMVGTVEEIADDKSSNFYTLKLKPATNFFNIEFVYVIANSQVVEQKRME
                     DSTHKKFQ"
     gene            1987..2520
                     /locus_tag="VGM24_12755"
     CDS             1987..2520
                     /locus_tag="VGM24_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY4156300.1"
                     /translation="MSELLKNIIRFILFIFIQVFILFKMPPLHRFISPYLYYLYILWL
                     PFSMSRAGLMSLAFLFGLTLDYFMHTPGLHAAACVLIAYLRPFVVNMLISQEGAEKNY
                     LSPSITSMGWAPYGTYVFVLTLFHHGYLVFLEWLSFGSFLYFIGKVVATTGISMLLIL
                     LTELLFFRKEKFRTNTV"
     gene            2539..>3982
                     /locus_tag="VGM24_12760"
     CDS             2539..>3982
                     /locus_tag="VGM24_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY4156301.1"
                     /translation="MSVFNQSRSNIVRLIFAGMFLIIFAQLFNLQVISDKYKKMAQEN
                     ALFPKRIYPTRGIIFDRHSRAILNNTIMYDLMVTPSQVKNMDTAYFCQLMEIDTAEFR
                     SRVIDARIRNGPFRPTIFEDLLPPDLYAKLEENIWRFPGFNLQERPVRVYPYDAGAHI
                     MGYVGEVDSNILRRSNYYYQLGDYVGRSGLEQFYEKQLMGQRGIEYWIKDNKNRLVGH
                     YANRDLDTPAIAGRNLHTYLDIELQELAEKLLANKVGAIVAIEPKTGGILAMASSPDY
                     DPNSLTGPDKQKNYGKLVLDVRAPLLNRAIKGLYQPGSTYKPLAALIGLDEGVITPAS
                     GIACTGFYYGCNKVYKCTEKNPGHAANLRLAIANSCNSFFYNAFRLTLDNPRLHGPRD
                     GLMKWKEYANKFGLGERVGVDLPSEDAGNIPDTLTYDKEYHGSWNSCTMVTMGIGQDK
                     MQVTPLQIANAMCIVANKGSFYIPHFVEKVD"
BASE COUNT         1052 a          941 c          995 g          994 t
ORIGIN      
        1 tttgttaatt cggattattt ccatcaattt tgcatgcatc gtaacagaat ttaaatctga
       61 ccttttaaaa tgggattatt caatttcctg acgcaagaga ttgctatgga tctggggaca
      121 gccaacaccc tgatcattca caatgatgag atcgtcgtaa acgaacccag tatcgtggct
      181 ctggaccgga ataatccgaa aacagtactc gctgtcggaa aaagggccct gatgatgcat
      241 gaaaaaacgc atgagagcat acggaccgtc cgccccctga aagacggggt tattgccgat
      301 tttaacgctg ccgagctcat gatccgggaa atgatcaaaa tgatctatcc caaaaaaccc
      361 ttatttcccc ccagctggcg aatgatgatc tgcattccct ccagcattac ggaagtggaa
      421 aaaagggccg tacgcgacag tgccgaacag gcgggggcca aagaggttta cctgattcat
      481 gagccgatgg cggcagccct gggcatcggg atagacgtgg aagaaccagt aggcaatatg
      541 atcatcgata tcgggggtgg taccaccggc attaccgtaa tcgcccttgc cggaatcgtc
      601 tgcgaccagt ccatccggat tgccggagat gagttcacgg ctgatatcat ggaggccctg
      661 cgccgctatc acagtttact gatcggggaa agaacggccg agcagatcaa aatacagatt
      721 ggtgcggctt tgaaagacct ggacaatccg ccggatgata ttccggtgaa tggtcgtgat
      781 ctggtaacgg gtattcccaa acagatcctg gtgagttatc aggaaattgc agaagcattg
      841 gacaaaagca ttttcaaaat agaagaagcc attctgaaag cactcgaaac gacgcctcct
      901 gaacttgccg gggacatcta ccggcgcggt ttgtacctta ccggcggggg agccctgctc
      961 aggggattgg ataaaaggct aagccagaaa atcaaacttc cggtgcatat tgccgatgat
     1021 ccgctgaaga gtgtggttag gggaacgggg atttccctga aaaactacga caggtacccc
     1081 tttgttatgc ggtagttctg atcatgctgt tttattatta taaattctag cacctgtgcg
     1141 caatgttttt ctcttcatca gaaagtacgc caacttcctt tttttcctgg tgttgcagat
     1201 catagcgctc tcttttctct tccgatacaa caaataccac gaagccgttt ttatgaattt
     1261 tgccggggaa atcaccggca gtctcgacca gcgttacagc acggtcgaat actatttcaa
     1321 attgaaaaag attaatgaac agctggcaac cgaaaatgca cggctgaacc agatgctgaa
     1381 ggaaaattat gagaggcccg atgcaaacgg caaactggtg atggatacgg tgcagcttga
     1441 ttccctccgc caggttcaaa aatatacctg gctcggtgcc agagtggtgg gaagtaccgt
     1501 aagcacccag accaatttta tcacgatcca tcgcgggtcc aaccagggcg ttcagccaaa
     1561 catgggcgta gtcagtccgc tgggaattgt gggcacggtc gtgaatgtca gccagaattt
     1621 tgccagtgtc atgaccatac tccaccggca gtataaagtc gtggtgaaat tgaagaatag
     1681 cggagaccgc ggcacggtcg agtgggacgg aatcagtccc agctttgtta cgttaaaaga
     1741 cattcccaaa agcgcgaaag ttcaaaaggg agattccgtg gttaccagcc cgacatcctc
     1801 tttgtttccg ggcaatatca tggtgggaac cgtggaagag atcgcagacg ataagagcag
     1861 caacttttat acactgaagc tgaagccggc cactaatttc tttaatattg aattcgtgta
     1921 tgtgattgcc aattcgcagg tcgtcgaaca aaagcgaatg gaggattcca cacacaaaaa
     1981 attccaatga gcgaattgct gaaaaatatt atccggttca tcctttttat ctttatacag
     2041 gtgttcatcc tgtttaagat gccaccgctg caccgtttta tctctcctta tttatattac
     2101 ctctatatat tgtggctgcc tttcagcatg tcccgtgccg gattgatgag cctggccttc
     2161 ctgtttgggt taaccctcga ctatttcatg catacgccgg gcctgcatgc agctgcctgt
     2221 gtactgatcg cttatctgag gccatttgtg gtgaatatgc tgatctccca ggaaggagct
     2281 gagaaaaact atttgtcccc gtccattaca agcatgggct gggcgccgta cggaacctat
     2341 gtttttgtgc ttaccctgtt tcatcacggc tatcttgttt ttctggaatg gctgagtttc
     2401 ggatcttttt tgtattttat cggtaaggtg gtcgcaacaa ccgggatcag catgttgttg
     2461 atactgctta cggagctatt attcttccgg aaggaaaaat tcaggacgaa tacagtataa
     2521 ttaaatccca tatccggaat gtctgttttt aaccaatcgc gcagcaatat cgttcgcctc
     2581 atctttgcgg ggatgttcct gattattttt gcgcagctct tcaatctcca ggttatttca
     2641 gacaagtaca aaaagatggc tcaggaaaat gcactttttc ccaaacgcat ttatccaacc
     2701 cggggcatca tcttcgaccg ccacagccgg gccatcctca acaacacgat catgtacgat
     2761 ctgatggtaa caccatccca ggtcaaaaat atggatacgg catatttctg tcagttgatg
     2821 gaaattgata ccgctgagtt ccgctcgcgc gtaattgatg cgcgcatccg caatggtcct
     2881 ttccgtccca ccattttcga ggatctgctg ccgcccgatc tctatgccaa gctggaagaa
     2941 aatatctggc ggttcccggg cttcaacctc caggaaaggc cggtgcgtgt ttacccctat
     3001 gatgcgggtg cgcacatcat gggttatgtg ggcgaagtgg attcgaatat tttaaggcgt
     3061 tcaaattatt attatcagct gggagactat gtcgggagaa gcggattgga gcagttttat
     3121 gaaaaacagc tgatgggcca gcgcgggatc gagtattgga tcaaggacaa taagaaccgg
     3181 ttggtcggcc actacgcgaa ccgcgacctg gatacgccgg ccatcgccgg aaggaacctg
     3241 catacctatc tcgatattga actgcaggaa ctggctgaaa aattacttgc aaacaaagtc
     3301 ggcgccatcg ttgccattga acccaaaacg ggaggaatcc tggccatggc ctccagtccg
     3361 gattacgatc caaattctct aaccggaccg gataagcaga aaaactatgg gaagctggta
     3421 ctggatgtaa gggcaccact gttgaaccgg gccatcaaag gactttatca gccaggatct
     3481 acctataaac cgctggcagc cctgattggc ctggatgaag gcgtgattac accggccagc
     3541 ggcatcgcct gcaccggatt ttattacggt tgcaacaaag tgtataaatg taccgagaaa
     3601 aatccgggac atgcagccaa cctcaggctg gccattgcca attcctgcaa ctcctttttt
     3661 tataatgctt tccggctgac gctggataat ccccggctcc acggaccgag ggacgggtta
     3721 atgaaatgga aagagtacgc caataaattt ggcctggggg agcgggtggg tgtggatctt
     3781 ccgagtgaag acgcagggaa tattcccgac accctgacct acgataagga atatcacggc
     3841 tcctggaatt cctgtacgat ggtgactatg ggcatcgggc aggataaaat gcaggtaacg
     3901 cccctgcaga tcgcgaacgc catgtgtatt gttgccaata agggatcttt ttacattccg
     3961 cattttgtag aaaaagtgga cg
//
LOCUS       DASYCU010000633         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2891501, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000633 DASYCU010000000
VERSION     DASYCU010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2891501"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VGM24_12765"
     CDS             complement(<1..983)
                     /locus_tag="VGM24_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156302.1"
                     /translation="MSVKKATLVFFVFLSLLTFSLMLAPGHNGDMPFYIAAVFSRSGS
                     TDAEALSRAKEVIRAEMPPGQAAAHINRLDQAEKNLLDFYRIKPLYISTVSVLHRMGI
                     PFIPATLIPSLLSFFLIGCIVFSWSSRVFDPLPALVFSILLMLINPSIILSRLSSPDP
                     LSNLFLFGCFYRIYFERDYRPTLLFLILSVFTRLDNIISVFILLTLMRKWPSRASKTR
                     IPLTVYLPVILLTAVAALWINYYFEGNFWWYTRISYIRSFAAYGHQVLIYFLSLSESF
                     FPALLLFCLLVIFKGRLKPGMKSARILISIGLILLVRFLMFPSFEERFQTA"
     gene            complement(1001..2590)
                     /locus_tag="VGM24_12770"
     CDS             complement(1001..2590)
                     /locus_tag="VGM24_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156303.1"
                     /translation="MPNRSSDTLFQLVKSLKKSEKRNFKLYVKRSSSSENLKIIQLFD
                     ALDKMEVYDEIQLLKKNKDFSKQQLSNSKAHLYRQILSSLRLIRNENDLAIQLHEQLD
                     QAEILYSQGLYLQSLKVLDRIKEYARGLHQISYVLQILFFEQKIESLHITRSMLDRAG
                     RLAGELKTANEQLRLTGSLSSLSLQLYSWYIQHGVARNEKDEKAVSAFFESQLPVLHP
                     SSVTFYSNLYLYQSYSWNAFIGQDFLSYYRYTRRWVDLFEKEPQMIAIETAHYIKGMH
                     NLLGAHFNLLNYPRFVETLNRFEAFFHSDPVQENDNNRIQTFVYLHIAKINRHFIDGT
                     FAEGLDLVPYIEKKLKEYEIYLDGHRILIFYYKFASLYFGSGDYEKTIKYLNKIINWK
                     MDLRTDLQCYSRLLHLIAHYELGNYELLEYLIKTVYRFMAKMENLSRVEEEIFRFLRK
                     SFKLSPKQLKPEFGSLLEKLKAYQKNRFETRAFVYLDIISWLESKIREVPVQEILREK
                     FLRRNSVKPASGILSSEPGVE"
     gene            2734..3906
                     /locus_tag="VGM24_12775"
     CDS             2734..3906
                     /locus_tag="VGM24_12775"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY4156304.1"
                     /translation="MKSDKIFIFDTTLRDGEQVPGCKLNTKEKLELALELEDLGVDII
                     EAGFPISSPGDFLSVTEISKIIKRATVCALSRAVEKDIEVAADALKNARRPRIHTGIG
                     TSDYHIKSKFNSNREDILGRAVQAVTKARNYVDDVEFYAEDAGRTDNEYLARVVEAVI
                     TAGATVVNIPDTTGYCLPHQYGEKIAYLVEHVKNIDRATISCHCHNDLGLATANSIAG
                     VIAGARQVECTINGLGERAGNTSLEEVVMIIRQHAHLGFTTDIKTRRLNPVSRLVADT
                     MRMAVQPNKAIVGANAFSHSSGIHQDGFLKNSQTYEIINPDEVGADGSKIVLTARSGR
                     SALAHRLHKIGYEFTRDEIDLLYVEFLRMADTKKEVGEEDLHKLAAVTAAPAGNVR"
     gene            3922..>4851
                     /locus_tag="VGM24_12780"
     CDS             3922..>4851
                     /locus_tag="VGM24_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY4156305.1"
                     /translation="MPRTLFEKIWDTHVVKTIQDGPSIFYIDKHFIHEVTSPQAFKGL
                     EQRGLRVFRPGQVVATADHNVPTLDQHLPIREELSRLQVDTLQKNCEKFGIELYGLGH
                     PYQGIVHVIGPELGITRPGMTIVCGDSHTSTHGAFGTIAFGIGTSEVEMVLATQCLMQ
                     NKPGLMRISVEGKLNKGVVSKDIILYIISKISASGATGYAVEYAGSAIRDLSMEARMT
                     ICNMSIEMGARCGMIAPDDTTFNYIKGRKFAPEGKDWDQSLACWKTLFTDEGARFDRE
                     LYIDASDIEPMITYGTNPGMGIGISGQVPAFRDV"
BASE COUNT         1313 a         1197 c         1261 g         1080 t
ORIGIN      
        1 aaggcggtct gaaaccgttc ttcaaaggat gggaacatca ggaacctcac caaaaggatc
       61 aacccgatgc tgatgaggat ccgggcagac ttcattcccg gtttcagtct gcctttgaaa
      121 ataaccagga ggcagaataa aagcagggcc ggaaaaaagc tctcactcag ggataaaaaa
      181 tagatcagca cctggtgtcc gtaagcggca aaggagcgga tataggaaat ccgggtatac
      241 caccagaaat ttccttcaaa ataataattg atccaaagcg cggcaacagc cgtcagcagt
      301 ataaccggca gataaacggt tagcggtatc cgtgtttttg aagcgcggga gggccatttt
      361 ctcattaagg tcagaagaat gaataccgaa atgatattat cgagccgggt aaagacggac
      421 aggatcagga aaagaagcgt cggccggtaa tccctttcaa aataaatccg gtaaaaacag
      481 ccgaaaagaa agagattcga caaggggtcc ggagaggata aacgggaaag gataatggac
      541 ggattgatca gcatcagcag gattgagaaa accagtgccg gcaaaggatc aaaaacccgg
      601 gacgaccagg aaaatacaat gcatccgatc aggaaaaaag acagcaggga gggaataagc
      661 gtagccggaa tgaagggaat tcccatccgg tggagaacgg aaaccgtaga aatataaagc
      721 ggctttatcc ggtaaaaatc cagcaggttt ttctctgcct gatcaagccg gttaatgtgg
      781 gcggcggcct gaccgggcgg catttccgcc cggatcactt ctttggcgcg ggacagcgcc
      841 tccgcgtccg tcgatccgga gcgggaaaac acagcggcta tataaaacgg catgtccccg
      901 ttgtgtcccg gcgcgagcat cagggagaag gtaagcagcg agagaaaaac gaaaaataca
      961 agagttgctt tttttactga catagcggaa aataaatggg ctattcaacg ccgggctctg
     1021 aagataagat tccggacgcg ggtttaacgg aattgcgccg cagaaatttt tcccgcagga
     1081 tctcctgcac cggcacttcc cggatcttgc tttccagcca ggatatgatg tccaggtata
     1141 caaaagcccg ggtttcgaac cggttcttct gatacgcttt taacttttcc agcaaactgc
     1201 caaattcggg cttcagctgt ttgggagaaa gtttaaagga tttgcgcaga aacctgaaaa
     1261 tttcctcttc cacccggctc aggttttcca tttttgccat gaaacggtat accgttttga
     1321 tcaggtattc cagcagttca tagtttccca gttcatagtg cgcaatcaga tgcagcaggc
     1381 gggaataaca ctgcagatcc gtacgcagat ccattttcca gttgatgatc ttgttcaggt
     1441 acttgatcgt tttttcataa tccccgctgc cgaaataaag agatgcaaac ttgtaataga
     1501 atatcaggat ccggtggccg tccagataaa tttcatactc cttcagtttc ttttcgatat
     1561 agggtaccag gtcgaggcct tcggcaaacg ttccgtcaat aaaatgccgg ttgatcttgg
     1621 cgatatgcag gtaaacaaag gtctgaatgc ggttattatc attttcctga accgggtccg
     1681 aatggaagaa ggcttcaaag cggttcaggg tttcaacaaa gcggggatag ttcagcaaat
     1741 taaaatgggc gcccagcaga ttatgcatgc ccttgatata atgggccgtt tctatcgcga
     1801 tcatctgggg ctccttttca aaaagatcaa cccagcggcg ggtatagcgg taataggaga
     1861 gaaaatcctg tccgataaag gcattccagc tataactctg atacaggtat aaattggaat
     1921 agaaggtaac ggaagaagga tgcagaaccg gaagctgcga ctcaaaaaat gcgctcaccg
     1981 ccttttcatc tttttcattc ctggccacgc cgtgctgaat ataccagctg tacaactgca
     2041 gggacaggct ggacaagcta ccggtcagcc ttagctgctc attggcggtt tttaattcgc
     2101 cggcaagccg cccggcacgg tccagcatgc tgcgggtaat atgaagcgat tcgattttct
     2161 gttcaaaaaa gaggatctga agcacataac tgatctgatg cagaccccgt gcgtattcct
     2221 tgatgcggtc gagcactttc aggctttgca gataaaggcc ctgactatac aggatctccg
     2281 cctggtcgag ttgttcatgc aactggatcg ccaaatcgtt ttcattgcgg atcagcctca
     2341 ggctggaaag gatctgccgg tacaaatgcg ccttgctgtt tgaaagctgc tgcttgctga
     2401 aatccttatt tttcttcagc aactggatct catcgtaaac ctccattttg tccagtgcat
     2461 cgaacaactg gatgattttc aggttttcgg acgaggaact ccgcttcaca tagagcttaa
     2521 aattccgctt ctccgacttt ttcagagatt taacaagctg gaacagggta tctgaggagc
     2581 ggttgggcat cgtattttaa actggtttaa tccttatccg gcctgcattt cagccgtcaa
     2641 acccgcgttt caagcctgta attgaaggcc gaatttgatt tttctgccgg gctaaacaaa
     2701 aataacttcg aattcactca ttcccaaaac gatatgaaat ccgataaaat cttcattttc
     2761 gacacgaccc tccgcgacgg agaacaggtt cccggatgca agctgaatac gaaggaaaag
     2821 cttgaactcg ccctcgaact ggaagacctt ggagtagaca tcatcgaggc gggatttccc
     2881 atttccagtc cgggagattt cctttccgtg accgaaatct ccaagatcat aaagcgcgct
     2941 accgtgtgcg ccctcagccg ggcagtggag aaagatattg aagtggccgc ggacgcgctg
     3001 aaaaatgccc gccgcccccg gatccatacc ggtatcggaa catccgatta tcatattaaa
     3061 tcgaaattca attcaaaccg ggaagatatt ctcgggcggg cggtccaggc cgtaaccaag
     3121 gccagaaatt atgtggatga cgtagagttc tatgcggaag atgccggccg gaccgataat
     3181 gaataccttg cccgggtagt ggaggcggtg atcacagccg gcgccacggt ggtgaatatt
     3241 ccggatacta ccggctattg ccttccgcat caatacgggg aaaagatcgc ctacctcgtg
     3301 gagcacgtaa aaaatattga ccgggcaacc atctcctgcc attgtcataa tgatctgggc
     3361 ctggctacgg ccaattccat agccggcgtc attgccgggg cccggcaggt cgaatgcacc
     3421 atcaacggac tgggcgaacg cgccggcaac acttcgctgg aagaagtggt gatgatcatc
     3481 cgtcagcacg cccatctcgg gttcacgaca gatatcaaaa cccggcgcct gaatccggtg
     3541 agccgcctgg tcgccgacac catgcgcatg gccgtacagc cgaacaaagc gattgtgggc
     3601 gccaatgcct tttctcattc ttccggcatt caccaggatg gatttctgaa gaattcccaa
     3661 acgtacgaga tcatcaatcc cgatgaagtg ggggccgacg ggtcgaagat tgtactcacg
     3721 gccaggagcg gaagaagtgc cctggcgcac cgcctgcaca agatcggata cgaattcacg
     3781 cgggatgaaa ttgaccttct ctacgttgaa tttctccgga tggccgatac gaaaaaggaa
     3841 gtgggtgaag aagacctgca caaactggcg gcggtaaccg ctgcaccggc cggaaacgtc
     3901 cgctaacgtt aaattaaacc gatgccaaga acattattcg aaaaaatatg ggacacccat
     3961 gtcgtgaaga ccatccagga cggtccctcc attttttata tcgataaaca ttttattcat
     4021 gaagtgacca gtccgcaggc gtttaaggga ctggaacagc ggggcctccg ggtgttccgt
     4081 cccggccagg tggtggccac tgccgatcac aacgtgccga ccctcgacca gcaccttccc
     4141 atccgggaag aactttcccg gcttcaggtg gatacgcttc aaaagaactg cgagaaattc
     4201 gggatcgaac tctatggcct ggggcatcct tatcagggca tcgtccatgt aatcggaccg
     4261 gagctcggca tcacccggcc gggaatgacg atcgtatgcg gagacagtca tacctccacc
     4321 cacggcgcat tcggcacgat cgcctttggc atcggcacca gtgaagtgga aatggtactg
     4381 gccacccagt gcctgatgca gaacaaaccc gggctgatgc ggatcagcgt ggagggcaag
     4441 ctgaacaagg gagtcgtatc aaaggacatc attctctata tcatatcaaa aatttccgcc
     4501 agcggagcca ccggttatgc cgttgaatat gcgggctccg caatccgtga tctgtcgatg
     4561 gaggcgcgca tgaccatctg caatatgagc attgaaatgg gcgcccggtg cgggatgatt
     4621 gcccccgatg acaccacttt caattatatc aagggaagaa agtttgcgcc cgaaggaaaa
     4681 gattgggatc aatcgctggc ctgttggaaa accttattta cggatgaagg tgcgcggttc
     4741 gaccgggaac tctatatcga tgcctctgac atcgaaccca tgatcaccta cggcaccaac
     4801 ccgggcatgg gaatcggcat ttcgggccag gtgccggctt ttcgggatgt g
//
LOCUS       DASYCU010000634         3380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1150449, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000634 DASYCU010000000
VERSION     DASYCU010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150449"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VGM24_12785"
     CDS             complement(<1..900)
                     /locus_tag="VGM24_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY4156306.1"
                     /translation="MIPKKFVPLVLVALSVFSSVSAQVKTNITVLRQAALQAAAKQHD
                     MAGRLLRLSRQNRWPLFVSLRNGNRAVLYGIDPKGRPLYVATNDNIISAATIGTNQLW
                     PGGSTGLNLNGSTVPAGKIAVWDGGRVRGTHQELSGRILQKDNPDELNDHSTHVSGTL
                     IASGVNPAAKGMSFGAKQLVAYDFNNHLTEMFNEAPNLLISNHSYGTIAGWNFNADEN
                     RWEFSAAPGDTADFKFGYYSDEAQVWDSIAYNAPYYLIVKSAGNNRDVNGPEVGQPYY
                     RNNSSGVMIPAGNRPAGLSSNNGY"
     gene            999..1454
                     /locus_tag="VGM24_12790"
     CDS             999..1454
                     /locus_tag="VGM24_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4156307.1"
                     /translation="MLPKKPLVWIALGLLVTLLPGFRQARRTGIEGYIFKLSGNRMPS
                     PDAKLSPPKGIAASLYIYELTNLSQLTREGESPFYSNIKTRLVKTIQSDASGYFSVSL
                     PPGQYSLFTKKDALFYANYFDGQNNVAPVKVSPGKVTQVNVNVDYEASY"
     gene            1548..>3380
                     /locus_tag="VGM24_12795"
     CDS             1548..>3380
                     /locus_tag="VGM24_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY4156308.1"
                     /translation="MLTFALRMSNEVLLNLYENSSRLFQLADRITMSRPQHLQLKNLH
                     GSASAFVLAGIFNLETTSQLNHLLICEDSESAAYLHNSVENLTRGLDIFYFPSSFKNS
                     KSYRQLNSSHVMLRTEALTRWSAGGNKKMLVTYPEALFEKVVLPETLAGNMIRIKSGE
                     TIDPTALMESLVGFGFERSDFVYEPGQFALRGGILDIYSFGNEKPYRIELFGNEVDSI
                     RIFDPESQLSERRLLLVNIIPNIETLFETEKKVSLFEFLPENTVIWMHDRRFIREKLE
                     IQAAELKVFLESGAGVETDAGGDAPVKKTISADDFISLDSFDACTVSRHLVEFGHYRS
                     LDSGVFPIEFHTQPQPVFNRQFDRLIRDLESWESKKFRIFLFAENPKQLERLHTIFED
                     LKSGIQFEPIATSIHEGFIDEDLKIVCYTDHQIFQRYHKYRIKQAYNKSKAITLRTLR
                     ELQPGDYVTHIDHGVGIFSGLQKMEVNGRVQEAVRILYKDSDILYVNINSLHKISKYT
                     GKDGTVPKVSKLGSDAWQKLKEKTKTRVKEIAFDLIKLYAARKAQQGFAFSPDNYMQT
                     ELEASFIYEDTPDQSKSTADVKKDMESGSPMDRLVCGDVGFGKTE"
BASE COUNT          848 a          836 c          852 g          844 t
ORIGIN      
        1 ataaccgtta ttgctgctaa gccctgccgg ccggtttcct gccggtatca tgaccccgct
       61 ggaattgttg cggtaatagg gttggccgac ttccggaccg ttcacatccc ggttattgcc
      121 ggcggatttt acgatcaggt aataaggggc gttgtaggca atagaatccc agacctgcgc
      181 ttcgtccgaa taatagccga atttgaaatc agcggtatct cccggggccg cactgaattc
      241 ccaccggttt tcatcggcat tgaagttcca gccggcaatg gttccgtagg aatgatttga
      301 gatcagcagg ttgggcgcct cattaaacat ttctgtcagg tgattattaa aatcataggc
      361 aaccagttgc ttggcgccga aagacatgcc tttggccgcc ggattcaccc cggaagcgat
      421 cagggttccg gaaacatggg tggaatgatc gtttaactca tccgggttat ccttttgcag
      481 gatcctgccg gacaattcct ggtgtgtgcc gcggaccctt cccccatccc atacggcgat
      541 tttgcctgcg ggaacagtgg agccattcag gtttaatccc gtgctgccac cgggccaaag
      601 ctggttggtg ccaatggtcg ctgcggaaat gatattgtcg ttggtggcta cataaagcgg
      661 ccggcccttg ggatcaatac cgtaaagaac ggcgcggttt ccatttcgga gggaaacgaa
      721 aagcggccac ctgttttgcc gggaaagacg aagcagtctc ccggccatat catgctgttt
      781 ggcagcagcc tggagggcgg cctggcggag tacggtaata ttcgttttga cttgtgcaga
      841 aacactggaa aagacagata aggcaactag aaccagtggt acaaacttct tgggtatcat
      901 gaggttcatt aaatggtaaa taccttttaa aattacatat ttttaatcag caccgggttt
      961 tttgatttaa ctttcagtta gttagatatc aacggcgtat gctacctaaa aaaccacttg
     1021 tatggattgc cctcggcctc ctggttacgc tgcttcccgg attcaggcag gcgagaagaa
     1081 ccggcataga agggtatatt ttcaagctgt cgggcaaccg gatgccctcc cccgatgcaa
     1141 aacttagtcc gcccaaaggc atcgccgcct ccctttatat ttacgaactg accaatctca
     1201 gccagttgac ccgggaaggt gaaagcccct tctacagcaa tatcaaaaca cggctcgtaa
     1261 aaaccatcca atccgatgcc agcggttatt tttcagtatc ccttcctccc ggtcagtact
     1321 cgctgtttac caagaaggat gctttattct atgccaatta ttttgatggt cagaacaatg
     1381 tggctccggt aaaagtaagc ccgggcaagg ttacacaggt taatgtgaac gtggattacg
     1441 aagcaagcta ttaaaatccg gttctgaaac ggtgaatccg tttccctttc tccttccctc
     1501 cccattggcc cggccctcca ttgaatactg caaaactaag cccccggttg cttacctttg
     1561 ccctccggat gagtaatgag gtgttattga acttgtatga aaattcatcc cgactttttc
     1621 agctggcgga caggatcact atgtcccgcc cccagcacct tcagctgaaa aacctccacg
     1681 gaagcgcctc cgcttttgtc cttgccggga tcttcaatct cgaaacgacc agccagttga
     1741 accacctgct gatctgtgag gattcggaat cagccgctta tctgcacaat tcggtggaaa
     1801 atctgaccag gggactggat atcttttatt ttccttcttc ttttaagaat tcgaagtctt
     1861 accggcagct taacagcagt cacgtgatgc tccgcaccga agccctgacc cgctggagcg
     1921 ccggaggcaa caagaaaatg ctggtcacct acccggaagc cctgtttgaa aaggtggtgc
     1981 ttccggaaac cctggccgga aacatgatcc gcattaaatc gggcgaaacc atcgatccca
     2041 ctgccttgat ggaatccctg gtcggtttcg gatttgaaag atccgatttt gtatatgaac
     2101 cgggacagtt tgctttgcgc ggaggcatcc tggatatcta ttctttcggc aatgaaaagc
     2161 cttaccggat tgaacttttt ggaaatgaag tggactcgat ccgcattttt gaccccgaat
     2221 cgcagcttag tgaacgcagg ttgttgctgg tcaatatcat tcctaatata gaaaccctgt
     2281 tcgaaacaga aaaaaaggta tctctctttg aattccttcc ggagaatacg gtgatctgga
     2341 tgcacgaccg gcggttcatc cgggaaaaac tggagatcca ggcggctgag ctaaaggtat
     2401 ttctggaatc gggcgccgga gtggagacgg atgccggcgg ggatgcgcct gtaaaaaaaa
     2461 caatttccgc ggatgatttt atttccctgg attcttttga tgcgtgcacg gtttccaggc
     2521 acctggtgga attcggccat taccgctccc tggactcagg agttttcccc attgaattcc
     2581 atacgcagcc gcaacccgta tttaaccggc aattcgaccg gctgatcagg gatctggaaa
     2641 gctgggagtc gaagaaattc cggatctttc tttttgcgga aaatccaaaa caactggaaa
     2701 gactgcatac tattttcgag gacctgaaat cgggcattca gttcgaaccc attgcgactt
     2761 ccattcacga gggatttatt gacgaggacc tgaagatcgt ttgttatacg gatcatcaga
     2821 ttttccagcg gtatcataag tataggatca agcaggcgta taataagagc aaggccatta
     2881 cccttcggac gcttcgcgag ttgcagccgg gcgactatgt aacgcatatc gatcacgggg
     2941 ttggcatatt cagcggattg cagaagatgg aagtaaacgg tcgcgtccag gaagcggtcc
     3001 ggatcctgta taaggacagt gatatccttt acgtgaatat caattccctg cataagattt
     3061 cgaagtatac cggcaaggac ggcaccgtgc ccaaagtgag taagctggga agcgatgcct
     3121 ggcaaaagct gaaggaaaaa accaagacca gggtaaagga aattgccttt gatctcatca
     3181 aattatacgc tgcccgcaag gcgcagcagg gttttgcatt ttctcccgac aattacatgc
     3241 agaccgagct cgaggcatcc tttatttatg aggatacccc cgaccagagt aaatcgaccg
     3301 ccgacgtgaa aaaggatatg gaatccggat ctcccatgga ccgcctggtt tgcggggatg
     3361 tggggttcgg caaaacggag
//
LOCUS       DASYCU010000635         4103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2746925, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000635 DASYCU010000000
VERSION     DASYCU010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2746925"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..916
                     /locus_tag="VGM24_12800"
     CDS             143..916
                     /locus_tag="VGM24_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY4156309.1"
                     /translation="MYQTIITSLEENIFIIHINRPDKLNALNRTVIRELGEAVDEVYQ
                     QDTVRAAIITGSGNRAFVAGADISGFAGLSREEGRELAREGLDVFFKIENSPKPIVAA
                     VNGFALGGGCELAMACHFRLCSETAQFGQPEVNLGLIPGYGGTQRLTRLIGKGRAMEA
                     LLTGNLINAPLALQYGLVNYITTPEKLMEKTKLILIQIISKAPLAIAKCIRAANAAAG
                     NEEGYALETEGFGECFGTNDMREGVAAFLEKRKPVFKGS"
     gene            962..>4103
                     /gene="treS"
                     /locus_tag="VGM24_12805"
     CDS             962..>4103
                     /gene="treS"
                     /locus_tag="VGM24_12805"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY4156310.1"
                     /translation="MYVGPPLNDKLHWYKDAILYELHIKAFRDGNGDGIGDFEGLLQT
                     LDYLQDIGVTAIWLLPFYPSPLKDDGYDIADYYQIHPAYGNIEQFKQFLDEAHNRNLK
                     VITELVINHTSDQHPWFQKARRSPKGSPERNYYVWSDDPSQYKDVRIIFQDFEPSNWS
                     WDPVAEQYYWHRFFHHQPDLNFENPEVQEEILKIISYWCRMGVDGFRLDAVPYLFERE
                     GTNGENLPETHVFLKKLRKHVDEHFPSTVFLAEANMWPEDAAAYFGNGDECHMNFHFP
                     LMPRMFMALQMEDRYPVTDIFDQTPAIPDTCQWAIFLRNHDELTLEMVTDEERDYMYK
                     VYAKDPKARINLGIRHRLAPLLKNNRHKIELLNCLLFSLPGTPVIYYGDEIGMGDNFY
                     LGDRDGVRTPMQWSRDRNAGFSTANPQQLYLPLILDPEYHYESVNAETQRLNTSSLLW
                     FMKRIINMRKRYKAFGRGNLNVLNLENPKVFAFTRTYEEQTLLVVVNLSKFPQPAEVD
                     LSDYKGYSLIEVVSRNKFPKVKEDGCYFFTLAAYAFHWFALEKMRDELHSGSELPFLE
                     ISRWEDMQQLSNVGKLESEILPGYLPKTKWFNGLHKNLYTLKISNHEIISFSENPVYW
                     ILFDVVYQSGLPETYQLPLRFVPDQEREKMRELHPDSLIAVMECNGKRGFLCDAFFTS
                     SLQQDLINRMVSQQSLPLQLGKMDFYGGHSLKEHWKETDEIKTRIHTSDELNTSITYD
                     NRFLLRMYRKIDISINPDLEITRFLTEKAKFRYMPQFIGAIEWKYDQKAIVLGIMQTM
                     IENHGDGYTYMRERVNNFIERVMARDKSQLNPYEYIGTLVNPVSFDQLPEHLQELIGT
                     NTAGQTRLLGKRTGQMHLALASGMEDAFRPEPFSLHYQRSLYSHMQSLLREAFQNLTR
                     YEKKATGEPAKKAALILSQRNILVEEMKKIYTKKLDVIKIRIHGNYHLSQVLLTGKDL
                     AIQDYGGNAAHSYSERRLKRSALRDVASMVTSIYEVARETFLNNDHVSKESPAGLLPF
                     ASLWAHYISGIFINSWK"
BASE COUNT         1103 a         1011 c         1041 g          948 t
ORIGIN      
        1 catcattcct gaacaggata taaaaaggct tcaggaaatg ggcgttggcc ggttatttgc
       61 tcccggcacc aataccacag agatcgctga atatatccgg gaatgggtgc tggaaaataa
      121 atggaacgca aacaactgaa agatgtacca gacaataata accagtctcg aagaaaatat
      181 ttttatcatc cacattaacc ggcccgataa attgaatgcg ctgaacagaa cagtgatccg
      241 ggaactgggg gaagcggtgg acgaagtata tcagcaggac acggtccggg cggcgatcat
      301 tacgggttcc ggaaacaggg catttgtggc cggtgcggac atcagcggct tcgccggact
      361 aagcagggaa gaaggccgcg agctggccag ggaagggctg gacgtttttt ttaaaattga
      421 aaattcgccc aaacccatcg tggccgccgt gaatggtttt gccctgggcg gaggttgcga
      481 actcgccatg gcctgtcatt tcaggctctg cagcgaaacg gcgcagttcg gtcagccgga
      541 agtaaacctc ggactgatac cgggttatgg cggaacgcag cggctgaccc ggttgatcgg
      601 aaagggaagg gcgatggagg ccctgctcac gggaaacctt atcaatgcgc cccttgcgct
      661 tcaatacggc ctggtcaatt atataacgac cccggagaaa ttaatggaaa agacaaagct
      721 gatcctgatt cagatcatca gcaaggctcc gctggccatt gcaaaatgca taagggcagc
      781 caatgccgct gccggaaatg aagaaggata cgcattggaa acggagggtt tcggcgaatg
      841 ttttggaacg aatgatatgc gggagggtgt ggcggcattc ctcgaaaaaa gaaagccggt
      901 atttaaagga tcctgaatgg gcctggcatg ctaattttac tcattacttt aaacctggaa
      961 tatgtatgtc ggtccgcccc tgaacgataa actgcattgg tataaggatg cgatcctcta
     1021 tgagttgcac atcaaggcct tccgggacgg taacggagac ggcatcggcg actttgaagg
     1081 acttctccag accctcgatt atctccagga tatcggcgta accgccatct ggctcctgcc
     1141 tttttatccg agtcccctga aagacgacgg atatgatata gcggattatt atcagatcca
     1201 tcccgcttac gggaatattg agcagttcaa acaattcctg gacgaagccc ataaccgcaa
     1261 cctgaaagtg atcacggaac tggtcatcaa ccatacttcc gaccagcatc cctggttcca
     1321 gaaggcacgc cggtcaccca agggttcccc ggaaagaaat tattacgtgt ggtcggatga
     1381 cccctcccaa tacaaggatg tgcgcatcat tttccaggat tttgagcctt ccaactggag
     1441 ctgggatccg gttgcggagc aatattactg gcaccgtttc tttcatcatc agcccgatct
     1501 gaattttgag aatcccgagg tacaggaaga gatcctcaaa attatttctt actggtgccg
     1561 catgggggtg gacggtttcc ggctggatgc cgttccctat ctgtttgagc gggaaggaac
     1621 gaatggggag aatcttcccg aaacgcatgt atttctgaag aaattaagaa aacatgtgga
     1681 tgagcatttt ccttcaaccg tatttctggc cgaagccaat atgtggccgg aagacgctgc
     1741 ggcctatttc ggcaacggcg atgaatgcca tatgaatttc catttcccct taatgccccg
     1801 gatgttcatg gcattgcaaa tggaagaccg ctatccggta acggatattt ttgatcagac
     1861 gcctgccatc ccggatacct gccagtgggc gatttttctg cgtaaccacg atgagctcac
     1921 gctggaaatg gtaacggacg aagaacggga ttacatgtac aaggtctatg caaaagatcc
     1981 caaggcccgg atcaacctgg gcatccggca ccggctggca ccgctgctga aaaataaccg
     2041 ccataaaata gaattgctga actgtctgct tttttcgcta cccggaaccc cggtgatata
     2101 ttatggcgat gagatcggga tgggggataa tttttatctc ggcgaccggg acggggtaag
     2161 aacgcccatg caatggtcgc gcgaccggaa tgccggattt tccactgcca atccccagca
     2221 attatacctg ccccttattc ttgatccgga atatcattat gaatcggtga atgcggaaac
     2281 gcagcgtctg aatacttctt ccctgctctg gtttatgaag cggatcatca atatgcggaa
     2341 gcggtacaaa gccttcgggc ggggtaatct gaatgtgctc aacctcgaga atccgaaagt
     2401 atttgcattc acccgtacgt atgaggaaca gaccctgctg gtggtcgtga acctttccaa
     2461 attcccccag cctgcagaag tggatctttc ggactacaag ggatattccc tgatcgaagt
     2521 ggtgagccgg aacaaatttc ccaaagtgaa ggaagacggc tgttattttt tcaccctggc
     2581 cgcctatgcc ttccactggt ttgccctcga gaaaatgcgg gatgaattgc attccggctc
     2641 cgaactgcct ttccttgaaa tatcccggtg ggaggacatg caacagcttt ccaatgttgg
     2701 aaaactggaa tcggagattc tgcccgggta ccttcccaaa acaaaatggt tcaacgggct
     2761 tcataaaaac ctctacacct taaagatcag caatcacgaa atcatatctt tcagcgaaaa
     2821 cccggtgtac tggatcctct tcgatgtggt ctaccagagc ggattacccg aaacctatca
     2881 gctgccgttg cgttttgttc cggaccagga aagggaaaag atgcgggagc ttcatcccga
     2941 ttccctgatc gccgttatgg aatgcaacgg gaaaagagga tttctctgtg atgcattttt
     3001 tacttcctcc cttcagcagg atcttatcaa ccggatggtg agccagcagt cgcttcccct
     3061 gcagcttgga aaaatggatt tctacggcgg gcattcgcta aaagaacatt ggaaagaaac
     3121 cgatgagatc aaaacgcgca ttcatacctc ggatgaatta aatacttcta tcacctacga
     3181 caatcgcttt ctcctgcgga tgtaccgtaa aatagatatt tccatcaatc ctgacctgga
     3241 gatcacccgt ttcctgacgg aaaaagcaaa attccggtat atgccccaat tcatcggggc
     3301 gatcgaatgg aaatacgatc agaaagcgat tgtgctcggt atcatgcaaa cgatgatcga
     3361 gaatcatggg gacggatata cctatatgag ggaaagagtg aacaatttta ttgaacgcgt
     3421 gatggccagg gacaaatcac agctcaatcc gtatgaatac ataggaaccc tggtgaatcc
     3481 ggtgagtttc gatcagctgc cggaacattt gcaggaactg atcgggacca atacagccgg
     3541 tcaaacccgg ctgctgggta aacggaccgg acaaatgcac ctggccctgg cttccggcat
     3601 ggaagacgca ttcagaccgg aacccttttc cctgcattat cagcgatctc tctactcgca
     3661 tatgcagtct ttactccggg aagcctttca gaatctgacc cgctacgaga aaaaagcgac
     3721 cggcgagccg gcgaaaaagg cagcgctgat cctctcccaa cggaatatac ttgtagagga
     3781 gatgaaaaag atctacacaa aaaaactgga tgtgatcaag atccggatcc acggcaacta
     3841 tcatttatcc caggtattgc ttacgggaaa agacctggcc atccaggatt atggcggaaa
     3901 cgccgcccat agctatagcg agcgccggct gaaacggtct gccctgcggg atgtggcatc
     3961 catggtcacc tctatctatg aagtagcccg ggaaaccttt ctgaacaacg accatgtttc
     4021 aaaagaatcc cctgccggcc tgcttccttt tgcttccttg tgggcacact atatcagcgg
     4081 gatctttatc aattcctgga aag
//
LOCUS       DASYCU010000636         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1150998, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000636 DASYCU010000000
VERSION     DASYCU010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150998"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VGM24_12810"
     CDS             <1..749
                     /locus_tag="VGM24_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY4156311.1"
                     /translation="NELKYPWMDEGFTQYAGARVWGFLNGDSSAAQTFSHEYNNYKIL
                     AKSNFEEPLSTYADFYNSNAGYNYSSYYKGAVFMEQLAYITGDQVLDKILLEYYREWR
                     FRHPDANDFMRVAEKVSGLQLGWYKDFWINSTKKVDYAIDSLWEVNGKTRIRLRMIGK
                     VPMPIDVGLQFKDGSRELAYIPQYLMFGVKPEEDKSVPRVVYEPWKWTSPEYEFEISH
                     RLTDLKTIEIDPSQRMADVNRSNNRLDLKW"
     gene            complement(768..1430)
                     /locus_tag="VGM24_12815"
     CDS             complement(768..1430)
                     /locus_tag="VGM24_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY4156312.1"
                     /translation="MSITVCIVDDHPGIRNALEHLILCADGFDLAGSCSGFREAVIRI
                     PALRPQVVLMDINLGENENGIDIIRELKPNYPELLFMMCTVYEEDEKIFDALNAGASG
                     YILKKTHPDRLLEAIRELKEGGAPMSSLIARKVINVFRYTTCAQGIWPGSFSGKARPL
                     SVLSNRENEILEQLSLGLLYKEIAANLFISQETVRKHVYHIYEKLHVNNRVEAINKFF
                     RR"
     gene            complement(1536..2186)
                     /gene="rsmG"
                     /locus_tag="VGM24_12820"
     CDS             complement(1536..2186)
                     /gene="rsmG"
                     /locus_tag="VGM24_12820"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY4156313.1"
                     /translation="MEIVTRYFSGFTEEQLAQFAGLESLYTQWNARINVISRKDIGSL
                     YEKHVLHSLSLAAVFNFRRGSRVIDIGTGGGFPGIPLAIFFPETHFHLVDSIGKKLNV
                     VDAVSESLGLKNVSTRHIRAEEIQERKFDVAVSRAVAPLKDLWAWSKALLKNQPPPKG
                     VDGSAEGLPFAGGLICLKGGDLAMEISESNTRPKIREIYPLFPEEVFRGKYIVQLR"
     gene            complement(2177..>2803)
                     /locus_tag="VGM24_12825"
     CDS             complement(2177..>2803)
                     /locus_tag="VGM24_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEY4156314.1"
                     /translation="YGPYRKRPGLLNKLIRFETFHSALQYLSFALAGHPYMGVGRNLS
                     YKRELFFKNKGFSSVNHLPGGDDDLFINQVADRRNTRIVIDAESFTLSEPKKTFREWV
                     QQKTRHYSTGKFYRPKQKWLLGIYSVSQFLFYPAFVISLIYAHWSWTLAVFALRFFLQ
                     GIVYFLSMKKLNESDLFPFWWLLDIWMIGYYLIFAPALLWKKPRMEWR"
BASE COUNT          740 a          691 c          688 g          684 t
ORIGIN      
        1 ccaatgaatt aaaatatccc tggatggatg aaggatttac ccagtacgcc ggggcccgcg
       61 tctgggggtt tttaaacgga gacagttcgg ctgcccagac cttttcccat gaatacaaca
      121 attataaaat cctggcaaaa agcaatttcg aagagccttt gagcacgtac gcggattttt
      181 acaattccaa tgcgggatat aattattctt cttattacaa gggggctgtt tttatggaac
      241 agctggccta tatcaccggc gatcaggtgc tggataaaat cctgcttgaa tattaccggg
      301 agtggcggtt caggcatccc gatgccaacg attttatgcg tgttgcggaa aaagtgagcg
      361 gtctgcagct gggttggtac aaggacttct ggatcaacag caccaagaaa gtggactacg
      421 cgatagacag tctgtgggaa gtgaatggaa agacccggat ccgtttacgc atgattggca
      481 aagtgccgat gcccatcgat gtggggttgc agtttaagga tggcagccgt gaactggcgt
      541 atattcccca atacctgatg tttggcgtga aacccgaaga agacaagtcc gttcctcggg
      601 ttgtttatga gccatggaaa tggaccagtc ccgaatatga attcgagatc agtcaccggc
      661 ttacggatct gaaaacaatt gaaatagatc cttcacaaag gatggcggat gtgaaccggt
      721 cgaataaccg gctggatcta aaatggtgaa taccgcaccg gatcccctca ccttctgaaa
      781 aatttgttga tggcctccac ccggttatta acatgcagtt tttcatagat atgataaacg
      841 tgtttgcgga cggtttcctg gctgatgaag aggttggccg caatttcctt atacagaagc
      901 ccaagggaga gttgttccag gatctcattc tcccggttcg ataaaacgga caggggcctt
      961 gctttcccgg aaaaggatcc cggccagatc ccctgggcgc aggtggtata ccggaagaca
     1021 ttaatcactt tccgcgcgat caggctgctc atgggcgctc ctccttcctt cagttcccgg
     1081 atggcttcca gcaggcggtc gggatgcgtt ttcttcagaa tataaccgct cgcgcccgca
     1141 ttcagcgcat cgaatatttt ttcgtcttct tcatatacgg tacacatcat aaacaaaagc
     1201 tcaggataat tcggcttcag ttcgcggatg atgtcaatcc cgttttcatt ttctcccagg
     1261 ttaatatcca tcagcaccac ctgcggccgc agggccggaa tccggatgac cgcttcccgg
     1321 aatccggaac aactgcccgc cagatcaaaa ccatctgcac acagaatcag atgctccagc
     1381 gcattgcgga ttcccgggtg atcgtcaact atacataccg tgatggacat ggcctcgaat
     1441 ataaaaatca ggaacgtcta aatttacctg accggccatt tgataaaaca gtaaaatcac
     1501 cctgaaacaa taccggcttt ccatttggca ggtattcagc gcagctgcac aatatatttt
     1561 ccccggaaga cctcttcggg aaaaagcgga tagatctccc ttatttttgg ccgcgtattg
     1621 ctttcggaaa tttccatagc cagatcgcca ccctttaaac agatcagtcc gccggcaaag
     1681 ggaagtccct ctgctgaacc atccaccccc tttggagggg gctgattttt gagcagggct
     1741 ttggaccagg cccatagatc ctttaaaggg gccaccgccc gggaaacggc cacatcgaat
     1801 tttctttcct gaatttcttc tgcccggata tgccgggtgc tcacattttt caaaccgagg
     1861 gattcgctta ccgcatccac cacatttagt ttcttgccga tgctgtccac caggtgaaaa
     1921 tgggtttcgg ggaaaaatat agccagcggg atgcccggaa accctccacc ggtgccgatg
     1981 tcgataaccc ggcttccccg gcggaaattg aaaaccgcag caagactcag ggaatgcagc
     2041 acgtgctttt cataaagact gccgatatct tttcgagaga tcacattgat ccgggcattc
     2101 cattgggtat agagggattc cagcccggca aactgagcca gttgttcttc cgtaaaaccg
     2161 gagaaatacc gggtgactat ctccattcca tgcggggctt cttccataat aatgcagggg
     2221 caaaaatgag ataatatccg atcatccaga tatccagcaa ccaccagaat ggaaagagat
     2281 ccgattcatt cagttttttc atggataaaa aatagacgat gccctgtaaa aaaaagcgaa
     2341 gggcgaatac ggccagggtc caggaccagt gcgcatagat caggctgatg acaaaggccg
     2401 ggtaaaataa aaactggctg accgaataaa tgccgagcag ccatttctgt ttgggccggt
     2461 agaatttccc ggtactgtaa tgccttgttt tctgctgaac ccattcccgg aatgtttttt
     2521 tgggttctga taaagtgaag gactccgcgt caataacgat ccgggtattt ctccggtcgg
     2581 cgacctgatt gataaacagg tcgtcgtccc ctcccggcaa atggttcacg gaggagaagc
     2641 ccttgttttt gaaaaataat tcccgcttat aagaaaggtt tcttcccacg cccatatagg
     2701 ggtgaccggc cagggcaaag gacaaatatt gcaatgcgga atgaaaggtt tcaaaacgga
     2761 tcagcttatt gagcaaaccg ggccgcttcc ggtaaggacc gta
//
LOCUS       DASYCU010000637         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4633182, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000637 DASYCU010000000
VERSION     DASYCU010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4633182"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VGM24_12830"
     CDS             complement(<1..947)
                     /locus_tag="VGM24_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY4156315.1"
                     /translation="MLRHLQIQNYAIMEEISMDLTSGLQVITGETGAGKSIMAGALSL
                     VLGERADNTVLMHRDKKCIVEASFGVEGKKEIAQFLAENGLDTEPELILRREISANGK
                     SRAFINDTPVNLDQLRRLSIQLVDLHQQFDMILLGESGFQMSVLDALAGHSAQLKEYR
                     QGFQQLRLLQKDLAELKEKQARADKENDYNTFLYTELQEAGLQENEFEKAEADLKLLS
                     HSEGIKSVLAGIHFEMQLSEQPLVQQVRMIAQQLEPYAAYHPEFPEIIRRIQSAYIEL
                     QDIAGEVESVNEDIRYDPAQIETLNERIHTGYRLFKKHNV"
     gene            complement(988..2499)
                     /locus_tag="VGM24_12835"
     CDS             complement(988..2499)
                     /locus_tag="VGM24_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4156316.1"
                     /translation="MFFRGRFPGLLICLSLLMGCLPGNPVFAQYHYDFNPVCQKAYGQ
                     IIALRLDSGKALIEAEKKTDPRNLIPYFLENYIDFFTLFFNEDPAEYQRRKPGEDQRL
                     NLMKKGDPSSPFFLFTRSVIYFQWAAADIKFGNRWEAAWSFRRSFLNGKENLVKFPGF
                     QPSRMLQGAMQVAAGTIPPGYQWLGDLLGIQGSIAEGMKVLENVLTGSDRFARLFHDE
                     AVFYFLYLKFHIQNQPGEVFQYIRQHQLDTRNNHLFTYLAANLYRNNQQAASAEQVIR
                     NRNPDPGYLDMPVWDLEMGYALADHLDPGAIMYLERFLRRFKGKFYVREVLQKLGWIY
                     YLQGQKEKAENARARIFRMGNSETEADKQALKDAQSGRWPNELLLKARLLDDGGYFHE
                     ALQLLSGKSSSSFNNPADQLEFAYRLGRLYDALNRKQEAIDAYLTTIKLGEHRKEYYA
                     ARAALQTGYIYEARGDKKTAIPFFQKVLALKGHDYKNALDQKAKAAIERCNNG"
     gene            complement(2537..3292)
                     /locus_tag="VGM24_12840"
     CDS             complement(2537..3292)
                     /locus_tag="VGM24_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY4156317.1"
                     /translation="MPNVKEFSVEEKLASLISLQKIESKLDEYKVLKGELPMEVSDLE
                     DEILGLNARQTRIEEEINGIQEFISQKKQGMKDAEELIKKYDKQSQNVKNSREFEAIN
                     KEIEMQQLEVKLSEKHVKDANEEVAEKVILLDKARKGIASKETVLKNKKEELDKIIAA
                     TEKEERHFAKLSAEAREKIEPRLLHSYDRIRTNYRNGLAVVPVVRDACGGCFNAIPPQ
                     RQSEIRQRKKIIVCENCGRILVDNDLNDVVEVK"
     gene            complement(3296..4096)
                     /locus_tag="VGM24_12845"
     CDS             complement(3296..4096)
                     /locus_tag="VGM24_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY4156318.1"
                     /translation="MKISAVTSLLESLAPPAFQEDYDNSGLQTGDLEKECSGVLVSLD
                     TTEAVIEEAVQKGCNLLISHHPLIFHGLKQISRETGTGKAILTAVKNDITVYAIHTNL
                     DNVLPGVSSTIADKLGLRNRSILAPKPPGETGGLQEAGSGLIGNLERPLAEKQFLDLL
                     REKFGVPVVRHSPLTGKAVNRVALCGGSGSFLIPNALAQGADFFVSADIRYHSFFEAE
                     GSLVIADIGHYESEQFAVDLLCDVILKKFPNFAVLKAGTATNPVHYYL"
     gene            4162..4791
                     /locus_tag="VGM24_12850"
     CDS             4162..4791
                     /locus_tag="VGM24_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY4156319.1"
                     /translation="MKNTLFMLATLAIFAFTATPKTNSGKAGTLTPEERAFMVKYMTE
                     SRDALIADVAGLSPNQLSFKASPERWSVADCVEHIALAETKLTPAVKQGLSAPAEPGR
                     RDSIRITDEQLMNGLVDRSHKFQAPEMLRPSGNFKTTQEALDVFVEQRNKNIEYVKTT
                     NDDLRNHFFMHPAVGTIDIYQGMIFITGHSKRHTLQLEEVKADPNFPKN"
     gene            complement(4851..>5197)
                     /locus_tag="VGM24_12855"
     CDS             complement(4851..>5197)
                     /locus_tag="VGM24_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156320.1"
                     /translation="GVGFGGGAGGVIAVYTKKGSERRADPMIKGLDQARIIGYSPIRQ
                     FYSPDYLRHPEDLTQNVRTTLYWNPYVLTEKGNTKLSFTFYNNDVTHKLRVVLEGFNQ
                     EGKLTRVEKIIE"
BASE COUNT         1188 a         1374 c         1281 g         1354 t
ORIGIN      
        1 acgttgtgct ttttaaagag ccggtatccc gtatggatgc gttcattgag ggtttcgatc
       61 tgcgccggat catagcggat atcctcatta acggattcca cctcgccggc aatatcctgc
      121 aattcgatgt aggccgactg aatccggcgg ataatttcgg gaaattccgg atgataagca
      181 gcataaggct ccagctgctg ggcgatcatc cggacctgct gaaccagcgg ctgttccgat
      241 aattgcattt cgaaatggat gccggccaga acggatttaa tgccttcgga atgactaagc
      301 aatttcaaat ccgcttccgc tttttcaaat tcattttcct gcaaaccggc ttcctgcagc
      361 tcggtgtata aaaacgtatt ataatcattt tctttgtccg cccgcgcctg tttttctttt
      421 aactccgcga gatctttttg caataaacgc aattgctgaa aaccttgccg gtattccttc
      481 agctgggcgg aatgaccggc cagggcatcc agcacggaca tctgaaaacc cgattctccc
      541 agcaggatca tatcgaattg ctggtgcagg tccacgagct ggatgctcag cctgcgaagc
      601 tggtcgagat ttaccggcgt atcgttgatg aatgccctgg atttcccatt ggcgctgatc
      661 tcccggcgaa ggataagttc cggttccgta tccagaccgt tctctgcaag gaactgcgcg
      721 atctcctttt taccttccac cccgaaggac gcttccacaa tgcatttttt atcccgatgc
      781 atcagaacgg tattgtccgc acgttctccc aacaccaggg agagtgcgcc ggccataatg
      841 gatttgcctg cgcccgtttc accggtaatg acctggagac cggaagtaag atccatggag
      901 atctcttcca taatcgcgta attctgtatc tgaagatgcc ggagcataaa atttctttcg
      961 gtgttcaatt aaatatagga aatattttca tccgttatta caacgttcga tcgccgcttt
     1021 tgctttctga tccagggcat ttttataatc gtgccctttc agcgcaagca ctttctgaaa
     1081 aaagggaatg gctgtttttt tatcgccccg cgcttcatag atataaccgg tttgcaaggc
     1141 ggcgcgggct gcataatatt cctttctgtg ctcacccagt ttaatagtgg tcaggtaggc
     1201 atcaatggcc tcctgcttcc ggttgagcgc atcatacaac ctgcccaacc ggtaggcaaa
     1261 ttccagttga tcggccggat tgttgaagga agaagaggat ttgccgctga gcagctgcag
     1321 ggcctcgtga aaatagccgc catcgtccag caggcgggct tttagcagca gttcgttggg
     1381 ccagcggccc gactgcgcat ctttcagcgc ctgcttgtcg gcttccgttt ccgaattccc
     1441 catccggaaa atgcgggcgc gggcattttc cgccttttcc ttctgcccct gcaaataata
     1501 gatccagccc agtttctgca atacctccct gacataaaat ttccccttga accggcggag
     1561 aaagcgctcc agatacataa tggccccggg atccaggtgg tcggccaggg cataacccat
     1621 ttccaggtcc cagaccggca tatccaggta acccggatcc ggattcctgt tccggatgac
     1681 ctgttccgca gaagccgcct gctggttgtt ccggtaaaga ttggctgcga gataggtgaa
     1741 caggtggtta ttcctcgtgt cgagctggtg ctggcggata tactgaaaaa cttctccggg
     1801 ctggttctga atatggaatt tcaggtaaag aaaatagaaa accgcttcat cgtgaaacag
     1861 ccgggcaaac cggtcggagc ccgtcagtac attttccaga accttcattc cctccgcgat
     1921 gcttccctgg atccccagca gatcgcccag ccactgatat cccgggggaa tggttcctgc
     1981 ggccacctgc atcgcgccct ggagcatccg ggaaggctgg aatccgggaa atttcaccag
     2041 gttttccttc ccgttcagaa aagacctgcg gaaagaccag gccgcctccc agcggttgcc
     2101 aaatttgata tcggctgccg cccactgaaa atagatcacg gaacgggtga acaggaaaaa
     2161 aggagacgag ggatcccctt tcttcatgag gttaaggcgc tggtcctctc cgggttttct
     2221 tcgctggtat tccgccggat cctcattaaa aaaaagggtg aaaaaatcaa tgtagttctc
     2281 gagaaaataa ggaatgagat tgcggggatc tgtttttttt tccgcttcga tcagggcttt
     2341 gccggagtcc agccggagcg ctatgatctg accataggct ttctggcaga cgggattgaa
     2401 atcgtaatgg tattgggcga aaacaggatt acccgggagg catcccatca gcagggaaag
     2461 acaaattaaa aggcccggaa atcgcccgcg gaaaaacatg accgcaagtt aaaggagtca
     2521 gccctccggc gcaaggttat ttaacttcca cgacgtcatt gaggtcgtta tccacgagga
     2581 tacgtccgca gttttcgcaa acgataatct ttttacgctg gcggatctcg ctctgacgct
     2641 ggggcggaat ggcattgaaa caaccgccgc aggcatcgcg gacaaccggc accacggcca
     2701 gtccgttccg gtaattggtg cggatccggt cataagagtg gagtaagcgg ggctcgatct
     2761 tttccctcgc ttccgcggag agttttgcaa aatgacgttc ttccttttcc gtcgccgcaa
     2821 tgatcttgtc gagttcttct ttcttgttct tcagaacggt ctccttgctg gctatgccct
     2881 ttctggcctt gtccaggagg atcacttttt cggccacttc ctcattggcg tccttaacat
     2941 gtttctcaga caacttcacc tcgagctgct gcatttcaat ttctttattg atcgcttcaa
     3001 actcacggct gttcttgacg ttctggctct gcttgtcgta cttcttgatc agttcttcgg
     3061 catccttcat tccctgcttt ttctgggaaa tgaattcctg aataccgttg atctcttctt
     3121 cgatgcgggt ctgccgggcg ttgaggccca ggatctcatc ttccaggtcg ctcacttcca
     3181 tcggaagctc tcctttcagt actttatatt cgtctaactt ggattctatc ttttgaaggg
     3241 aaatgagtga ggctaacttt tcttcgacag aaaattcttt aacgttgggc atagtttata
     3301 aataataatg gacgggatta gttgcggttc ccgctttaag gacggcaaag ttaggaaatt
     3361 ttttgaggat aacatcacag agcaggtcca ccgcgaactg ctcgctttca taatgaccga
     3421 tatcggcgat caccaggctg ccttccgcct caaaaaacga atgatagcgg atatccgcgc
     3481 tgacaaagaa atcagctccc tgagccaagg cattgggaat caggaagcta cctgagccgc
     3541 cgcagagggc cacccggttc accgctttgc cggtcagggg actgtgccgg accacgggca
     3601 ccccgaactt ctcccggaga aggtccagga attgtttttc cgcgaggggc cgctccaggt
     3661 tcccgataag ccctgacccg gcttcctgaa ggcccccggt ttccccaggg ggtttgggcg
     3721 ccagtatgct ccggttccgc aatcccagtt tgtcggcgat ggtgctgctc acccccggca
     3781 gaacattgtc caggttggta tgaatggcat aaacggttat atcgtttttg acggcggtca
     3841 ggatcgcttt cccggttccg gtttcccggc tgatctgttt aagtccgtgg aagatcaggg
     3901 gatggtggga gatcagcaga ttgcatccct tctgcacggc ttcttcaata accgcttccg
     3961 tcgtatccag gctgaccagg acgccgctgc actccttttc caggtctcct gtctggaggc
     4021 cgctgttatc gtaatcttcc tggaacgccg gtggcgcaag ggattccagg agactggtga
     4081 ccgctgagat tttcatttgc tgaaattaat atgtttttat ttttcacccg gaaacttcta
     4141 actttcgcaa aactgcagtt tatgaaaaac accttattca tgctggccac cctggccatt
     4201 tttgcgttta ccgcaacacc caaaacaaat tccggaaaag caggcacact tacacctgaa
     4261 gagcgcgcct ttatggtcaa atacatgacc gagagccggg acgcgcttat cgccgatgta
     4321 gccggacttt cgccaaatca gttaagcttt aaggcctctc cggagcggtg gagcgtggcc
     4381 gattgcgtgg agcacatcgc cctggcggaa acaaaactta ctccggccgt caaacaggga
     4441 ctttccgctc cggcagaacc gggcagaagg gattcgatca ggatcaccga tgaacagctg
     4501 atgaatggat tggttgaccg ctctcacaaa ttccaggcgc ctgaaatgct aaggccttcg
     4561 ggaaatttta aaaccaccca ggaggcgctg gatgtttttg ttgaacagcg aaataaaaat
     4621 attgaatacg tgaagaccac caacgacgat ttgcggaatc attttttcat gcatcccgcc
     4681 gtgggaacga tcgatattta tcagggcatg atctttatca ccgggcacag caagcggcat
     4741 acccttcagc tggaagaggt gaaggcggat ccgaatttcc caaagaactg atcccatccg
     4801 gctttaaaat aaaaaagtaa aaaggagttc cctttttact tttttattta tcattcaatg
     4861 atcttttcca ccctggtcag tttcccctcc tggttaaatc cttccaggac cacgcgcagt
     4921 ttatgcgtca cgtcattatt ataaaaagtg aaactgagtt ttgtatttcc tttttccgtg
     4981 agcacatagg gattccagta cagggttgtc cgcacatttt gcgtcagatc ctcgggatga
     5041 cggagataat ccggagaata aaactgccgg atgggggaat acccgatgat gcgtgcctga
     5101 tccagtcctt taatcatcgg atccgccctt cgttcactgc cttttttggt atagaccgca
     5161 atcactccgc cggctcctcc gccaaagccc actcctg
//
LOCUS       DASYCU010000638         9665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4919353, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000638 DASYCU010000000
VERSION     DASYCU010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4919353"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VGM24_12860"
     CDS             <1..764
                     /locus_tag="VGM24_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEY4156321.1"
                     /translation="NQFERKPAPLDKLCVGVKCGGSDGFSGITANPAVGYCADLLVAL
                     GAKVLLAEFPELNGVEQELIDRSVSEPVAKKFIRLMNDYSAAAARVGSGFDMNPSPGN
                     IRDGLITDAMKSAGAAKKGGSSPVVDVLDYTEPATKAGLNLVCTPGNDVEATTGQAAA
                     GATLILFTTGLGTPTGNPVSPTIKISTNTPLAKRMADIIDIDTGVILEGEKTIEEMGE
                     EILRYCIRAAGGEVIPKAVLLNQHDFIPWKRGVSL"
     gene            781..2187
                     /gene="uxaC"
                     /locus_tag="VGM24_12865"
     CDS             781..2187
                     /gene="uxaC"
                     /locus_tag="VGM24_12865"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEY4156322.1"
                     /translation="MKQFLGSDFLLNTKTADRLYHEFAAPMPIIDYHCHLPAQQITDD
                     HRFGNITEIWLKGDHYKWRAMRTNGVNEQFITGEKNDWEKFQQWALTVPYTWRNPLFH
                     WTHLELQRYFHIDKILGPDTAKEIYEETNRKLRDPEFSVKNLLKSMQVELLCTTDDPV
                     DSLEQHRALKKVGFGIRVLPAFRPDNALRVENPALFNGYLEKLEKSSGVSIHSFQHFL
                     DALKNRHDFFHDSGCTVSDHGLEQLVAEDFTEAEIKGIFDKIRKGLPPDKLSVKKINT
                     AVLLQLAEWNHERGWVQQFHLGALRNVNRRLVDSLGADSGLDSIGDFQQGIVLAAFLN
                     RLDKRNRLAKTILYNVNPSDNEVMATMTGNFNDGTAAGKIQWGSAWWFLDQKDGMTKQ
                     LNALSNMGLLSRFIGMITDSRSFLSYPRHEYFRRILCDLFGTEIENGELPNDIPWAGK
                     MIRDICYYNAKNYFNWNA"
     gene            2222..3241
                     /locus_tag="VGM24_12870"
     CDS             2222..3241
                     /locus_tag="VGM24_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY4156323.1"
                     /translation="MGKTLCFGELLLRLSPAPQGEWLKTRTMPVFLGGAELNVASALG
                     NWNQPVSYCTALPDNFLSADISGAIRERNISTDGIFYSGSRLGTYFLSQGDDMKHAGV
                     IYDRDHSSFGELKRGMLDWDLILKDISWFHFSAISPALNASVAEVCREGLEAASKKNI
                     TISVDLNYRAKLWKERNPQDAMRVLCAYCDVIMGNIWSAQQLLGIPLQENQLDQARQE
                     SYLEQAEQASLEIMKRFPRCKTVANTFRFDWKEKGIEYYATLYSEIRHFFRSSVFRAE
                     TIVDKVGTGDCFMAGLIFGLLRHWPEEQIIQFAAAAAFGKFMETGDATAQPVEIIQKR
                     AAQYV"
     gene            3234..3899
                     /locus_tag="VGM24_12875"
     CDS             3234..3899
                     /locus_tag="VGM24_12875"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY4156324.1"
                     /translation="MYKKEEILQAILDQAVLPLYFHPDAERSVEIMKSLYAAGIRIIE
                     YTNRGPSAIENLKSLKLAIKKDCKGMMLAAGTITDGKTAELALNAGADFLISPGFVKE
                     VLAVSRKHDVLWIPGCMTPTELIKASHAEIRLVKLFPGSLLGPEFVSSVKDIFPDLMF
                     MPTGGVEPTMENLRAWFGAGVSAVGMGSRLITKSIIDKKEFAELTRQTEILLNQIDTI
                     RHS"
     gene            3922..5205
                     /locus_tag="VGM24_12880"
     CDS             3922..5205
                     /locus_tag="VGM24_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY4156325.1"
                     /translation="MRQPMGKYRWVVVTLLFFATTINYLDRQVIGLLKDTLARDFQWS
                     EKDYSDIVMAFTAAYAISLLGFGRLIDRIGTKLGYTISLIIWSVAAMLHALARGTLGF
                     GMARTALGLGEGGNFPAAIKAVAEWFPRKERAFATGIFNSGSNIAAFVGPPVIAWIFT
                     SYGWKEAFIWTGALGFIWLIFWWWLYDIPAKQKRLSAAEFQYIHSDVPDIEREPDAKV
                     RWQHLLGLRQTWAFIFGKFLTDPVWWFYLFWIPSYFNSTYHLNLQSSAIHVSTVYVVA
                     SFGSILGGYLSGWLIKRGMPVYKARKTTMLLVAFCVVPVFFVRYSTSIWPAVWLISLA
                     AAAHQAWSANIFTTASDMFPKRAVSSVVGIGGMAGSIGGVLFPLLIGVILDHFKLLGK
                     LGTGYNIIFLICSSAYLVAWLVMHFLSPKMKFVEM"
     gene            5238..6551
                     /locus_tag="VGM24_12885"
     CDS             5238..6551
                     /locus_tag="VGM24_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY4156326.1"
                     /translation="MNDFIAARSQMALSLAFHIVFSCIGMVMPFFMAVSHFYYLRTGK
                     PVYLNVTRAWSKGVAIFFATGAVSGTVLSFELGLLWPTFMKHAGPIFGLPFSLEGTAF
                     FIEAIALGFFLYGWGRFNKWFHWFTGLVVGISGLVSGILVVAANAWMNSPAGFEYQNG
                     KYFHIDPVKAMFNEAWFHQALHMCIAAFAATGFAVAGVHAYMILRNKNAAFHRKAFRI
                     AAVFACTAAVLQPFSGDHSAKEVAIHQPAKLAAMEAVFQTKRNSPLLIGGIPDEKNKT
                     VHYGIEIPGMLSFMISGNARTEVKGLDQIPEKDHPPVLITHLAFQLMVALGVLMFALS
                     IGYFFSLWKKRNWQEKRSLLKLFALATPIGFIAVEAGWTVTEVGRQPWIIYGFMRTAD
                     AVTPMPGIMYSFYLFTAVYFSLALIVCFMLYRQIKMVPVLYDREE"
     gene            6599..7612
                     /locus_tag="VGM24_12890"
     CDS             6599..7612
                     /locus_tag="VGM24_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY4156327.1"
                     /translation="MLYVVIIYLWMALLLYLLLGGADFGAGIIELFTSDKNKDKTSNT
                     LYRAIGPVWEANHMWLIIVIVILFTGFPEIYSTVSTYLHIPLAIMLLGIIARGTAFAF
                     RHYDAVQDEMQKVYNKIFRYSSFITPLFLGIIAGSAVDGRIDPKAGSFAGAFLFSWLS
                     FFSVSVGLFTVSICGYLAAIFIIGEARTEAGRKRYIVKARSMNLAAVFAGSLVFIAAT
                     RSNIPLASWLFGNPVGLCAILAALLSLIVQWWLISRGKIIIIRALAGFQIAMILLATT
                     YYHYPRIILMKGGAYLSLLEHRGDDKSITALALALLAGSLFILPALLYLIYSFQKKKP
                     VGI"
     gene            7704..8030
                     /locus_tag="VGM24_12895"
     CDS             7704..8030
                     /locus_tag="VGM24_12895"
                     /inference="COORDINATES: protein motif:HMM:NF025862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 C-terminal
                     domain-containing protein"
                     /protein_id="HEY4156328.1"
                     /translation="MELLGSVPTTSDETSILDGKVGEYIVTAGKNKGNWFVGALTDWS
                     ARDISLNFDFLDQGTYKMTLCKDGVNADRYAADYLLTDSPVKKGDLIKIHLAPGGGFL
                     LRLERQ"
     gene            complement(8050..>9665)
                     /locus_tag="VGM24_12900"
     CDS             complement(8050..>9665)
                     /locus_tag="VGM24_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HEY4156329.1"
                     /translation="NYKFKDPGSYSVWLIAKDGSCSDTTEKFNFPVYDPTIDGTVSLE
                     NAECYQQTRAIVRIKICNYGYAPVPPGTAVSFYDADPKSDTAHKLSPVFYTPDPVEGN
                     CCFTFTDTVNVSKPGLNQLYAVFNDNGSGSPLSLPNTNLPETNYTNNISSKSDFQFHV
                     QSLPDSATLQPGDSVLLSAKAGPGQVASYVWSSGQDLSCTACDSTYFLAAYRVYNMTK
                     KVVATSSNGCTDSAFTVLHMPPADDYRISLESLACAGEDSMHARFMICNNFIRGNIPQ
                     QLKVSFYDADPTGPGAHFLGPVFSTAEANADRCSAYEGFFKRTTTGKVFAVVNNDGQN
                     GTAASGILYEEARSDNNWDSLSAIPFVVTLSPSDTSISRLTSIRLNPQIEGGQALAFK
                     WQPAQYLSCSDCSSPVATPANSIDYRLTVQNEYACTATGTASIKTFSGGSVNIPTGFT
                     PNNDGHNDVFYVLGSEEVKMLKDFSIFNRWGQKVFQVTNASANDPRFGWNGLLNGRPA
                     DTGTYVYFVTIAFADGTTRLFKGTITLIR"
BASE COUNT         2310 a         2350 c         2591 g         2414 t
ORIGIN      
        1 taaatcagtt tgaaagaaaa cccgctccgc tggataaatt gtgcgtgggg gtaaaatgcg
       61 gcggaagcga cgggttcagc ggcattacgg ccaacccggc cgtcggctat tgtgcagacc
      121 tgctcgttgc attaggggcg aaggtattgc tcgcggaatt tccggaactc aacggagtgg
      181 agcaggaatt aattgaccgg tcggtgagtg agccggtagc aaaaaaattc atccggctga
      241 tgaatgatta ttcggcggct gccgcccggg tcggttcagg attcgatatg aatccttccc
      301 ccggaaatat ccgggacgga ttaattaccg acgcgatgaa gtctgccggc gccgccaaaa
      361 agggcgggag ctcaccggta gtggatgtgc tcgattacac agagcctgcc actaaagccg
      421 gactcaacct ggtttgtacg cccggtaacg atgtggaagc aactaccggg caggcagctg
      481 ccggagccac gctgatcctg tttaccaccg gcctgggaac gccgaccggg aatcccgtga
      541 gtccgaccat taagatttcg accaatacgc cgctggccaa aagaatggct gatatcatcg
      601 atatcgatac cggcgtgatc ctggagggag agaaaacgat agaagaaatg ggagaagaaa
      661 ttttaaggta ttgtatccgt gcggccggcg gggaggtgat tccaaaagcg gtattgctga
      721 atcaacacga ttttattccc tggaaaaggg gagtttcatt ataaattttt attccctttc
      781 atgaaacagt ttctgggaag cgatttttta ctgaatacga aaactgcaga ccggctctac
      841 catgaatttg ccgctccgat gcctattatt gactaccatt gccatttgcc tgcgcagcaa
      901 ataacggatg atcaccggtt tggaaatata acggagatct ggctgaaagg ggaccattat
      961 aaatggcgcg ccatgcgcac caacggcgtg aacgagcaat tcatcaccgg cgaaaaaaac
     1021 gattgggaaa aatttcagca atgggcttta accgttccct atacctggcg caatccgctg
     1081 tttcactgga cccatctgga actacagcgg tatttccaca tcgataaaat actgggcccg
     1141 gacacagcga aagaaattta tgaggagacc aacaggaaac tccgggaccc ggaatttagc
     1201 gttaagaacc tgctaaagtc catgcaggta gaattactct gcaccacgga cgatccggtg
     1261 gattcgctgg aacagcaccg ggcattaaaa aaggtaggat ttggaatccg cgtgcttcct
     1321 gctttccgcc cggataacgc cctccgggtt gaaaatccgg cgctgttcaa cggctatctg
     1381 gaaaagctcg aaaaatcgtc cggcgtttcg atccattcct ttcaacattt tttggatgcg
     1441 ttgaaaaacc gtcatgattt ttttcacgat tccggttgca cggtttccga tcacggactt
     1501 gagcagctgg tggcggaaga ttttacggaa gcagaaataa aaggcatttt cgataagata
     1561 agaaaagggc tgccgcccga taaactttcc gtgaaaaaga tcaatacggc cgtactgctt
     1621 caattggctg aatggaatca cgagagggga tgggttcagc aattccatct cggcgccctg
     1681 cgcaatgtca accgccggct cgtggactcg ctcggcgcgg acagcggact ggattccatc
     1741 ggcgactttc aacaggggat cgtgctggct gcattcctga accggctgga caagcgtaac
     1801 cggctggcaa aaacaatcct gtataatgtc aatccatcgg ataatgaggt aatggccacc
     1861 atgaccggca acttcaacga cggaacggca gcgggtaaga tacaatgggg ctcggcctgg
     1921 tggttcctgg atcagaagga cggaatgacc aaacagctga atgccctgtc caatatgggt
     1981 ctgctcagcc ggttcatcgg catgatcaca gattccagga gctttctctc ttatccaaga
     2041 catgaatatt tccggcggat cctctgcgat ctgttcggaa cagaaattga aaatggcgaa
     2101 ttgccaaacg atattccctg ggcggggaaa atgatccggg atatctgcta ttataatgcg
     2161 aagaattatt ttaactggaa tgcatgaatt cagggcccgg ttgcggaatt aaaataatac
     2221 catgggaaaa actttatgct tcggagagct gctgctcagg ctttcaccgg cacctcaggg
     2281 tgaatggctg aagacccgga cgatgcccgt ttttctcgga ggcgctgagc tcaacgtggc
     2341 ttcggctctg ggcaattgga atcagccggt ttcctattgt acggccctgc cggataattt
     2401 tctttcggcg gatatctccg gagccatccg ggagagaaat atttctaccg atggcatttt
     2461 ttattccggc agccgcctgg gaacttattt cctttcccag ggagacgata tgaaacatgc
     2521 cggcgtgata tacgaccgcg accactcttc gttcggggag ttaaagcggg gtatgctgga
     2581 ctgggacctt atcttaaagg atatttcctg gtttcatttc agcgcgatca gcccggcgtt
     2641 aaatgcatcc gtggcagaag tctgccgcga aggattggaa gcggcttcca aaaaaaatat
     2701 aaccatctcg gttgatctca actacagggc caaactttgg aaagagagaa atccgcagga
     2761 tgccatgagg gttttatgcg catactgcga tgtgatcatg ggaaatatct ggtcggctca
     2821 gcagctgctg ggaattccat tgcaggaaaa ccagctggat caggcccggc aggaatctta
     2881 tctcgaacag gcagaacagg cttccctgga gatcatgaaa agatttccgc gctgtaaaac
     2941 ggtggccaat acgttccgct ttgactggaa ggagaagggg atcgaatatt atgcaaccct
     3001 gtattcggag ataaggcatt ttttccggtc ttctgtattc agggcagaga ccatcgtgga
     3061 taaagtggga acgggggatt gttttatggc gggcctgata ttcgggctgc tccggcactg
     3121 gccggaagaa cagatcatcc agtttgcggc ggcggcggca tttgggaaat ttatggaaac
     3181 cggggacgcc accgcccagc cggttgaaat cattcagaaa agagctgcac agtatgtata
     3241 aaaaagaaga aatcctccag gccatcctgg accaggccgt cctgcccctg tactttcacc
     3301 cggatgcgga acggagcgtt gaaatcatga agtcattgta cgcggccggc atccgcataa
     3361 ttgaatatac caaccgggga ccgtctgcta ttgaaaatct gaaaagcctc aaactggcca
     3421 taaaaaaaga ttgcaaagga atgatgctgg ccgcgggtac gataacggat ggaaaaacgg
     3481 cggagctggc actgaatgcc ggcgccgatt tcctgatcag tccgggtttt gtaaaggaag
     3541 tgctggccgt ttcccgaaaa catgatgtgc tctggattcc aggctgcatg accccgacgg
     3601 aattgattaa agccagtcat gcggaaatcc gcctggtaaa attatttccc ggcagtctgc
     3661 tggggccgga gtttgtttca tcggtcaaag atatattccc tgatctgatg ttcatgccta
     3721 cgggcggagt tgagccaaca atggaaaacc tcagggcctg gttcggcgcc ggtgtttctg
     3781 cggttggtat gggaagcagg ctgatcacaa aatccatcat agataaaaaa gaatttgcgg
     3841 aattaacgag gcagacagaa atcctcttaa accaaattga tacgataaga cattcctgat
     3901 tttttaaacc agtctccgac gatgcggcag ccaatgggaa aatacagatg ggtagtggtt
     3961 accctgcttt tttttgctac cactataaat tacctggaca ggcaggtgat cggcttactg
     4021 aaagatacgc tcgccaggga ttttcagtgg tctgaaaaag attacagcga tatcgtaatg
     4081 gcttttaccg ctgcgtatgc cattagcctg cttgggtttg gaaggctgat cgaccggatc
     4141 ggcacaaagc tgggttatac catatcactc attatctgga gcgtggcggc catgctgcat
     4201 gcgctggcca ggggaacgct gggttttggc atggcgagaa cggcactggg attgggagaa
     4261 ggaggaaatt ttcccgcagc catcaaggca gtggcggaat ggttccccag gaaggaacgg
     4321 gcattcgcca ccggcatttt taacagcggc tccaatattg ccgcatttgt cggcccgccc
     4381 gtgattgcct ggatcttcac ttcttacggc tggaaggaag cgtttatctg gacgggcgcc
     4441 ctgggtttca tctggttgat attctggtgg tggttatacg atatcccggc aaaacagaaa
     4501 agactgtccg cagcagaatt tcagtatata cacagcgatg tgccggacat agaacgcgag
     4561 cccgatgcaa aagtcaggtg gcagcacctg ctgggcttgc ggcagacctg ggcttttatt
     4621 tttggaaaat tcctcacgga tccggtctgg tggttttatc ttttctggat cccgtcttat
     4681 tttaacagca cctatcacct gaacctgcaa agctcggcga tccatgtgtc caccgtatac
     4741 gtagtggcca gcttcggcag tattttgggc ggttatcttt ccggatggct gatcaaaaga
     4801 ggaatgccgg tttacaaagc aagaaagacc acgatgctcc tggttgcctt ttgtgtcgtt
     4861 cccgtgtttt ttgtgcgata cagcaccagc atctggccgg cagtctggct gatcagtctg
     4921 gccgcggctg cgcaccaggc ctggagtgcc aatattttca ccaccgcgtc agacatgttc
     4981 cccaagcggg cggtgagttc tgttgtcggt atcggtggca tggccggctc tatcgggggt
     5041 gtgctttttc ccttgctcat cggcgttatc ctggaccatt ttaaattatt gggcaagctg
     5101 ggaacgggat ataatattat ttttctgatc tgttcgagcg cttacctcgt tgcctggctg
     5161 gtcatgcatt ttctttcgcc gaaaatgaag tttgttgaaa tgtagcaata ccttcgtgca
     5221 accatcattc tctccacatg aatgatttta tcgcggcgcg gtcccagatg gcgctttcgc
     5281 tggcctttca cattgtcttt tcctgcatcg gcatggtcat gccctttttc atggccgtct
     5341 cgcactttta ttatctgaga accggaaaac cggtgtacct caatgtgacc cgggcctgga
     5401 gcaagggagt ggctattttt tttgctacgg gcgccgtttc cggaacggtg ctttcttttg
     5461 aactgggcct gctctggccg acctttatga aacacgcggg cccgatattc gggcttccgt
     5521 tttccctcga gggaacggct ttttttatag aggccattgc gcttggattt tttttgtatg
     5581 gctggggccg gttcaataaa tggtttcact ggtttaccgg gctggtcgtg ggcatcagcg
     5641 gattggtatc cggaatcctc gtggtagcgg ccaacgcctg gatgaacagc ccggcgggat
     5701 ttgaatatca gaacggaaaa tatttccata tcgatccggt aaaagccatg ttcaatgaag
     5761 cctggttcca ccaggccctg cacatgtgca tcgccgcgtt tgccgccacc ggatttgccg
     5821 tcgccggggt gcatgcgtat atgatcctgc ggaacaaaaa tgctgccttc caccggaagg
     5881 ccttccggat cgcagctgtt tttgcgtgta ccgcagctgt cttgcagccc ttcagcggag
     5941 accattcggc aaaagaagtc gccatacacc agcccgccaa gctggcggcg atggaggcgg
     6001 tatttcaaac caaaagaaat tctcccttac tgatcggcgg aattcccgat gaaaaaaaca
     6061 agaccgtgca ttacggaatt gaaattcccg gaatgctcag ctttatgatt tccggaaacg
     6121 cccggacaga agtaaagggc ctggatcaga ttccggaaaa agaccatccg ccggtactca
     6181 tcacacacct ggcttttcag ctgatggttg ccctgggtgt actgatgttc gccctcagca
     6241 tcgggtattt tttcagctta tggaaaaaac ggaactggca ggaaaaacga agcttattaa
     6301 agttatttgc gctcgcaacg cccatcgggt tcatcgccgt ggaagccggc tggaccgtca
     6361 ccgaggtagg gcgtcagccc tggatcattt acgggtttat gcgtacggcc gatgccgtta
     6421 ccccgatgcc cgggatcatg tattcttttt atctgttcac tgccgtttat ttttcactgg
     6481 cgctgatcgt gtgctttatg ctttaccggc agatcaaaat ggttccggta ttgtatgacc
     6541 gggaagaatg aaacggccgc cgcctgtgaa aagcggtgat attccataat gagcccttat
     6601 gctttatgta gtgatcattt atttatggat ggccctcctg ctctacctgc tgctcggggg
     6661 ggctgatttc ggtgcgggca taatcgaact ctttacttcg gataagaaca aagataaaac
     6721 cagcaacacg ctttaccgcg ccatcggtcc ggtctgggaa gcgaatcaca tgtggctgat
     6781 tatcgtgatc gtgatcttgt tcaccggatt tccggagatc tattccacgg tttccaccta
     6841 cctgcacatt cccctggcca ttatgctcct gggaattatt gcccgtggaa ccgcattcgc
     6901 ctttcgtcat tatgatgcgg ttcaggatga aatgcagaag gtctataata agatcttccg
     6961 ttattccagt ttcatcaccc cgttgttcct gggcatcatt gccggaagcg cagtggacgg
     7021 aaggatagat cccaaggccg gcagctttgc cggcgcattt ttattttcct ggctaagctt
     7081 tttctccgtt tccgtaggct tattcaccgt tagcatctgc ggatatctgg cagctatttt
     7141 catcatcggg gaagcgagaa cagaagcggg aagaaagcgc tacatcgtca aagcgcgatc
     7201 catgaacctg gcggccgtct ttgccggttc gctcgttttc attgccgcca cccggagtaa
     7261 tataccgctg gcctcctggc tttttggaaa cccggtaggc ctctgcgcca tcctggccgc
     7321 cctgctctcg ctgatcgtgc aatggtggct gatatcgagg ggaaaaataa tcatcatccg
     7381 ggcgcttgcg gggtttcaga tagccatgat cctgctggcc accacctact atcactatcc
     7441 gcggatcatt ctaatgaagg gcggggccta tctttctctc ctcgaacatc ggggagacga
     7501 caagtccatt acggcattgg cgctggccct gctggccggc agcctgttta ttctccctgc
     7561 cctcctgtac ctgatttaca gttttcaaaa gaaaaaaccg gtagggattt aatgattacc
     7621 agctggccat gtttgtcgtt tacgataacc ccatggaaat attctccggc aatccgtccc
     7681 aggcttagct ggaaccggaa ttcatggagc tcctgggtag cgttccgacc acctcggacg
     7741 aaaccagcat actggatggg aaagtggggg aatacatcgt gaccgcggga aaaaataaag
     7801 ggaactggtt tgtcggggcg ctgacggact ggtccgccag ggatatcagc ctgaattttg
     7861 atttccttga tcagggtacg tataaaatga ccctttgtaa agatggcgtc aacgcagatc
     7921 gctatgcggc ggattattta ttgacggatt cgccggtaaa aaaaggggat ctgatcaaaa
     7981 ttcacctggc tcccggcggc ggttttcttc tgaggctgga aaggcagtga ggcttatgaa
     8041 aactcctcct cagcggatca gggtgatcgt tcctttaaac aggcgggtcg ttccatccgc
     8101 aaaagcgatc gttacaaaat aaacatacgt gccggtatcg gccggtcttc cgttcaaaag
     8161 tccgttccag ccaaatcggg gatcattggc agatgcattg gtgacctgaa atactttctg
     8221 gccccaccgg ttaaatattg aaaaatcctt cagcatttta acctcctcgc tgcccagcac
     8281 ataaaagacg tcgttgtgac cgtcattatt gggcgtgaag cccgttggaa tatttacgct
     8341 tcccccggaa aaagttttta tgctggccgt tccggtggcc gtgcaggcat attcattctg
     8401 aaccgttaac cggtaatcta tactgttagc cggagtggcc acgggactgg agcagtccga
     8461 gcaggacagg tattgggccg gttgccattt aaacgcaagg gcctgccccc cttcgatctg
     8521 cggatttagc cggatgctgg ttaagcggct gatgctggtg tcggaaggac tgagggtcac
     8581 tacaaaggga atggcagaga ggctgtccca gttattgtcg gacctggctt cctcatacag
     8641 gataccggaa gcagccgtgc cgttctgtcc gtcgttattc accacggcga aaaccttacc
     8701 ggtggttgtc cgtttaaaaa aaccttcgta ggccgaacat ctgtctgcat tcgcctcggc
     8761 cgttgaaaac accggaccca ggaagtgcgc acctggtccc gtgggatccg catcataaaa
     8821 agatactttt agctgctgcg gaatattgcc ccgtataaaa ttattgcaga tcataaacct
     8881 tgcgtgcatg ctgtcttcac cggcacaggc caggctttcc aaactgatcc ggtagtcgtc
     8941 tgccggcggc atatgtaaaa cggtgaaggc actatccgta cagccgttag aactggtggc
     9001 caccactttt ttagtcatgt tatagactct gtacgcagca agaaaatatg tgctgtcgca
     9061 tgccgtgcaa cttaaatcct gaccagaact ccagacatac gaggcaacct gtccgggccc
     9121 ggcttttgcg cttaacagca cactgtcgcc gggctgaagg gttgcgctgt cgggaagcga
     9181 ctgaacatga aattgaaaat cacttttgga gctgatgtta ttcgtgtaat tggtttccgg
     9241 caggttcgtg ttgggcaggc ttaacggact gccgctgccg ttatcgttaa agacagcgta
     9301 tagctgatta aggccgggtt tactgacatt gacggtgtct gtaaatgtaa aacagcagtt
     9361 gccctccacg ggatcaggcg tataaaacac cggggataat ttatgggccg tatcgctttt
     9421 cgggtcagca tcataaaaag ataccgccgt gcctggcggt accggggcat acccgtaatt
     9481 gcagatcttg attcttacga tagcgcgggt ctgctggtaa cattcggcat tttccaggga
     9541 taccgtaccg tcaatggtag gatcatatac cgggaaattg aatttttcgg tggtgtcgct
     9601 gcaggaacca tcttttgcga tgagccaaac ggaataggaa ccggggtctt taaatttata
     9661 gttta
//
LOCUS       DASYCU010000639         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4344774, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000639 DASYCU010000000
VERSION     DASYCU010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4344774"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..>2906
                     /locus_tag="VGM24_12905"
     CDS             112..>2906
                     /locus_tag="VGM24_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEY4156330.1"
                     /translation="MTLKTTWLLAITLISYKVFFGQTPFPYEKTWKRIDSLIQKKGLS
                     KSALEEVNKLYAAAKKEKQEAQWVKAVIYRQQLLGTVDEDINRSRLQGEEEIRLAPPR
                     VSAWLKSLEAEQLYDYLRQNRYRLSDRTEVQNDSSEDMGTWTAGKLNKKIRELYLGSL
                     ENETLLQQTGLAGFDVLITKGNSRYLRPTLYDLLANRALTYFQNDDPEQPLAEDAFVM
                     DDPAIFLPGTQFMDHSFRSGGRSGNHLTALNIFQSLERFHSRDARPDARLDATVSRIA
                     FAYRYAVMPEKDSLYFSALRRITDQYPSLPAAAQAWYLQATFYSQNAASFDPLSDSSH
                     QYDYVKAKTICEQVLLQKDSSEGKTNCSTLLNNITRPAFSLETEKVNLPDQPFRILLT
                     YKNVGHLYARIIRVDDATVDALTVRGPDQKFWKKLTHFPVLKTFDQSLPETGDYQQHR
                     VEIRADALPPGQYALFTSSDPSFDDKAVLAAQFFFCSSIAFVNYGQDYFVADRNTGEP
                     LTELEVRSLYRVYDSRQQKYVFRKSKIYKTDQHGYFRLAPDKEDRYLERRLEFYHGKD
                     YLSSIGARVYSYSNTEEEGGKSDDARTYEKKMRKDILFTDRAIYRPGQTIYFKGLLVT
                     RDFLSGRYKIVTGSESRLYLLDANDQRVDSVLVSPDEFGSFHGSFHLPVNLLNGEFRL
                     RDSLTEDSKSFSVEEYKRPKFFVAYDPVKGSYRIGDTIHISGYAKAYAGNALDGSKVS
                     YRVFRETRFPYSWFFRRIPSNAEQEITHGESVTNAGGQFKIDFKANADQNIDKKSGPV
                     FSYRIETDITDLNGETRSAFTSVAASYRSFEIFSPLPSESRIELDSFHQIAVSTRNAS
                     GEFLPELLTLSFYRLKTPDRLIRKRYWDQPDQFVMTKEEYISSFPHDEYKNETSKASW
                     EKGKMVLEKTDSTRSNG"
BASE COUNT          778 a          705 c          720 g          703 t
ORIGIN      
        1 cccgttataa tttagttaaa gggtatggaa accggataag acaggcatcc ataggctgga
       61 tgcttcgtta aaccccacca tatgcccgct ttttggatta aaaagtatga tatgacctta
      121 aaaacgacct ggttactggc aattaccctt atcagctata aagttttttt tgggcagacc
      181 ccatttcctt acgaaaagac ctggaaaagg attgattccc tgatccagaa aaaaggcctt
      241 tcaaaatccg cactggaaga agtaaacaag ctctatgccg cggcaaaaaa ggaaaaacag
      301 gaagcgcagt gggtgaaggc cgtcatctac cggcagcagc tgctgggaac ggttgacgag
      361 gacataaaca gatcccggct gcaaggggaa gaagagatcc gtttggcgcc gccacgggta
      421 tccgcctggt tgaaaagcct ggaagcagaa cagctttacg attacctgcg gcaaaaccgg
      481 tatcgtttat cggacagaac cgaagttcaa aatgattctt cggaagatat gggcacctgg
      541 actgccggga aactgaataa aaaaatccgc gaactctacc tgggttcgct ggaaaatgaa
      601 acgcttttac agcaaaccgg tctggcgggc tttgatgtgc tgatcacgaa agggaacagc
      661 cgttacctgc ggccaacgtt gtatgacctc ctggccaacc gcgctctgac ctatttccag
      721 aatgatgatc cggaacagcc cctggcggaa gatgctttcg tgatggatga cccggcgatt
      781 tttttgccgg gcacacagtt catggatcat tccttccgct ccggcggccg gtccggcaat
      841 catctgaccg ctttaaacat attccaaagc ctggagcggt ttcattcccg ggatgcaaga
      901 cccgatgcac ggctggatgc aacggtcagc cggatcgctt ttgcttaccg gtatgcggtt
      961 atgccggaaa aggactcttt atatttttct gcgctccgcc gtatcacgga tcaatatcct
     1021 tccctgccgg ccgccgccca ggcctggtat ttacaggcta ccttctattc tcaaaatgcc
     1081 gcttccttcg atccgctatc ggacagcagc catcaatacg attatgtaaa agcaaagacg
     1141 atctgcgagc aggtgctttt gcaaaaagat tcttcagagg gaaaaacaaa ttgcagcacc
     1201 ctgctgaata atatcacccg gccagccttt tccctggaaa cggaaaaggt aaatcttccg
     1261 gaccagcctt tccggatcct gttgacgtat aagaatgtcg gtcatctcta tgcccgcatc
     1321 atccgcgtgg atgatgctac cgtcgacgca ttgaccgtgc ggggtccgga ccagaagttc
     1381 tggaaaaagc tgacccattt tcccgtgtta aaaacttttg accagtccct tccggaaacc
     1441 ggcgattacc agcaacaccg ggtggagatc agggcggatg cgctgccgcc gggccagtat
     1501 gccttattta ccagttcaga tccttctttt gacgacaagg ctgtgctggc cgcccaattc
     1561 tttttttgtt catccatcgc ttttgtgaat tacggccagg attattttgt ggctgatcgt
     1621 aataccggtg aaccgctgac ggagctggaa gtccggtccc tttaccgagt ctatgactcc
     1681 aggcagcaga aatatgtttt ccgtaaaagc aaaatataca aaaccgatca gcatggatat
     1741 ttccggctgg cgccggataa agaagatcgc tacctggaaa gaaggctgga attttaccat
     1801 gggaaagatt atctttcttc catcggcgca agggtttatt catacagcaa cacggaagaa
     1861 gaaggcggaa aatcggatga tgcacggact tatgaaaaaa aaatgaggaa ggatattctt
     1921 ttcacagacc gcgccatcta ccggccgggc cagaccattt atttcaaggg cctgctggtt
     1981 acccgggatt ttctcagcgg ccggtataaa atcgtaaccg gatcggagtc caggttatat
     2041 ctgctggatg ccaatgacca acgggtggat tctgtattgg tttctccgga tgaatttggc
     2101 tcttttcacg gaagtttcca tttgccggtc aatttattga atggggaatt tcgcctccgg
     2161 gattccctga cggaagattc caaaagtttt tcggtggaag aatataagcg cccgaaattc
     2221 tttgtggcct atgacccggt aaaaggatcc taccggatag gggataccat ccacatcagc
     2281 ggatacgcaa aagcctatgc ggggaatgcc ctggatggat cgaaagtcag ctaccgggta
     2341 ttccgtgaaa cccgttttcc atattcctgg tttttccgca ggatcccttc gaatgcagag
     2401 caggaaatca cacacgggga aagtgtaaca aatgccggcg gacagtttaa aatcgatttc
     2461 aaagcaaacg cggatcaaaa cattgacaag aaatccggtc cggttttcag ctaccggatt
     2521 gaaacggata ttaccgattt gaacggcgaa acaaggtctg cctttacttc ggtggctgcc
     2581 agctatcggt cgtttgagat cttttccccc cttccgtccg aaagccggat agaattggat
     2641 agttttcatc agatcgccgt cagcacccgg aatgcttcgg gcgaatttct gccggaactt
     2701 cttaccctta gcttttatcg cttaaaaaca ccggatcggc tgatcaggaa aagatattgg
     2761 gaccagccgg accagtttgt tatgacgaaa gaggaatata tcagctcctt tccccatgat
     2821 gaatataaaa atgaaaccag taaggcatcc tgggaaaaag ggaaaatggt gttggagaaa
     2881 acagacagta cccggagcaa tggttt
//
LOCUS       DASYCU010000640         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3038221, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000640 DASYCU010000000
VERSION     DASYCU010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3038221"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VGM24_12910"
     CDS             <1..307
                     /locus_tag="VGM24_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156331.1"
                     /translation="VFADNSIGPIVSTISIVIVFTLLSEMQIPLYDRTIKPFLFTSHM
                     LAWKGFFYTKVDETGSSLPGTIENFHAILKSFWVLIAYITGFVGSAIFIFNRKDILS"
     gene            317..1009
                     /locus_tag="VGM24_12915"
     CDS             317..1009
                     /locus_tag="VGM24_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156332.1"
                     /translation="MTRFLVLFPALLFFTPVLRAQDAGGYIKTIREKLEKINDYQADA
                     ELKTNIPFLRVPDAAVKVYYKKPDKIKIKNEKGISLVPRESVSISLYSLLNGQYRVID
                     AGTAKIGNIPVHLLKLLPENDNADLVLATLYIDAARLLVLKAKITTRENGTDEVNLSY
                     GKFASASLPDKIVFSFNTQDYKLPKGVTFDYDDGSQKNKSTGDRKNQRGKIEITFHSY
                     LINKGLPVDIFK"
     gene            1111..>1960
                     /locus_tag="VGM24_12920"
     CDS             1111..>1960
                     /locus_tag="VGM24_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEY4156333.1"
                     /translation="MADIGARVLSLEKFENILFKDEKLTLDKPALEKVDASFQFLKNF
                     STGKLIYGINTGFGPMAQYKVSDANRLQLQYNLIRSHSSGMGNLMSPLMGKAVLLARL
                     NSLMQAHSGIHPSLVELLAALINNDIIPCIYEHGGVGASGDLIQLAHVALALLGEGNV
                     LHEGNVRPAAEILKKFGLKPLEIHIREGISILNGTSAMTGIGLLNLIHARKLLHWSVV
                     LSAMTNEIVEAYDDHFSKELNMVKQHQGQNRIAAMLRHVLKDSKMIHSRSEHLYNPAN
                     IQHDVFE"
BASE COUNT          503 a          544 c          459 g          454 t
ORIGIN      
        1 ggtcttcgcg gataattcga tcggtcccat cgtatccact atcagcatcg tgatcgtatt
       61 caccctgctt tccgaaatgc agatcccttt gtacgaccgg accatcaagc cctttctgtt
      121 cacctcgcat atgctggcct ggaaaggttt tttctatacc aaagtggatg agaccggttc
      181 ctcccttccg ggaactatcg agaacttcca cgccatcctg aaaagtttct gggtgctcat
      241 cgcttatatt accggattcg tcggatcggc tatttttatt tttaaccgga aagatattct
      301 gagctaaatt ttacctatga ccaggttcct tgttcttttc ccggctctct tattcttcac
      361 cccggtgctc cgcgcccagg atgccggcgg atacattaaa acgatccggg aaaaactgga
      421 gaaaataaac gactaccagg cggacgcgga actgaaaacc aatatccctt ttttgcgggt
      481 gccggacgca gcggtgaaag tctattacaa aaaacccgac aagatcaaaa ttaaaaatga
      541 aaagggcatt tccctggtcc cccgcgaatc ggttagcatc agcctctata gcctgctcaa
      601 cgggcagtac cgggtgatcg acgcgggtac cgctaaaata gggaatatcc ccgtgcacct
      661 gctcaagctc ctgcccgaaa acgacaacgc cgacctggtg ctcgccaccc tatatatcga
      721 tgccgcccgc ctgctggtcc tgaaagctaa gatcaccacc cgggagaacg gcaccgacga
      781 agtgaacctg agctatggca aattcgcttc ggcttccttg ccggacaaga tcgtcttttc
      841 cttcaatacc caggactata aactgcccaa gggcgtcacc ttcgattatg atgatgggtc
      901 gcagaaaaac aaatccaccg gagaccggaa gaaccagcgc ggtaaaattg aaatcacttt
      961 ccattcctat ctgattaata agggattgcc cgtggatatt ttcaagtgat tcaaccctcg
     1021 acaggcccgt caaatcaaca aaaatttgac gtcttggcct tatttcgctt tttttgctct
     1081 ttggtgagac tttaaacaga ccgcacaaaa atggcagaca tcggagcccg tgttctttct
     1141 ctggaaaaat ttgagaatat actatttaag gatgaaaagc taaccctgga caagcccgcg
     1201 ctggaaaaag tggatgccag ctttcagttc ctgaaaaact tttctaccgg aaaactgatc
     1261 tatgggatca atacgggttt tggccccatg gcccagtaca aggtaagcga cgccaatcgc
     1321 ctccagctcc agtacaacct gatccgcagc catagctccg gcatgggcaa cctgatgagc
     1381 ccgctgatgg gaaaagccgt gctgctggcc cggctcaaca gcctgatgca ggctcattcc
     1441 ggcatccatc ccagcctcgt ggaattgctc gctgccttaa taaacaatga tattattcca
     1501 tgtatatatg aacacggcgg cgtgggcgcc agcggggacc tgatccagct ggctcatgta
     1561 gcgctcgccc tgctcgggga aggaaatgtg ttgcacgaag ggaatgtccg tccggccgca
     1621 gagatcctga agaaattcgg gctcaaaccg ctggagatcc atatccggga aggcatttcc
     1681 attctgaacg gaacctccgc catgacgggg atcggcctgc tgaacctcat tcacgcccgg
     1741 aaactgttac actggtccgt ggtgctttcc gccatgacca acgagatcgt cgaagcctac
     1801 gacgaccatt tttcaaagga actcaatatg gtgaagcagc accagggcca gaaccggatc
     1861 gcggccatgc ttcgccacgt gctcaaggac agtaagatga tccacagccg ctcggaacac
     1921 ctctataacc cggccaatat ccagcacgat gtttttgaag
//
LOCUS       DASYCU010000641         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_140686, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000641 DASYCU010000000
VERSION     DASYCU010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140686"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1324)
                     /locus_tag="VGM24_12925"
     CDS             complement(119..1324)
                     /locus_tag="VGM24_12925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY4156334.1"
                     /translation="MDNPALPYDEGKRLAALKEYRILDSSPEEAFDGITRLAAAICQT
                     AAAAILLIDSQSLWFKSVLGLEIRETPLNNTLVAEARNPGIFIVPDARKEERFAGKPL
                     IAGDPELVFYAGVPLVDPEGWLLGLLCVMDRKPGELSEMQQESLQMLAGQVLQLLKWR
                     KAALDIEKLKEDLDETKYELEQFTYVISHDIKSPLSSIVLSAEMLRENFGDAIDENND
                     QLLNVLSRASFKIRNLVEAVHSFYRGKRVLNEPAEPFELRSSIESVVLLLKAGRSAQI
                     NYPAEDVFMHGNRVALEQILINLLQNAIRYSDKEITVVDIRFRETESFYYFEVQDNGR
                     GIALADQQKIFEPFTSLGIPDRAGIRGTGLGLTTVKNLVEKQGGEIGLHSTPGEGTQF
                     EFSIRKNVR"
     gene            complement(1396..3216)
                     /locus_tag="VGM24_12930"
     CDS             complement(1396..3216)
                     /locus_tag="VGM24_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156335.1"
                     /translation="MEKSFIEVFFGRMNGKLYFGLVILVQLIFIFQGLDFTDSGFAAV
                     FYQRIFSDPSTVQYNFTYWLTGIIGGLWLKLFPGLGLLGLRIAGVICTTFTFAISYNL
                     LKKYLQTASLRLGLLLIVLFLGNGIKEFNYRDISALFFICAAWFLFTGLTRQKQFRIF
                     ISGMFVAANMFARLPNGLDFFLILAIVFSGYLNRIPLYRILFQCLLFLTGFLLMACGL
                     FLVMKDLHHDVFFFNNLEILRQTGRNQVNSRNLQNSLKLYLLQYSEGLTVSITVLVGL
                     WSFAAIWRRMKMEIYFLRGFWIFGKYGILVILTALCIIHAWVDGRSWYYLFLFYSGTS
                     LIIGFLIITGRQPKDMQLLSAIGCLMLLILPVGSHVVLMTVGKYAAWIMVPIAVDYLL
                     NIRSLSSSIVVSENNRHTYEQVINANQMNSLRNGFIFLTLTYILSVCYFYPHADRSNR
                     ARMRYEINNEHASYISTTPERAKTINELLEASSKYVRPDDWVLAYDCLPMYYFLTDSR
                     PFMHNSWVWLYDDAHFKTELTESLQETHVCPVVIMQKRSTLDNNWPRNDPDDSVIRKN
                     TRNDLQTFLKRYQYRQVWENDFFRLYVPAVKNLPVIASGL"
     gene            complement(3233..>3854)
                     /locus_tag="VGM24_12935"
     CDS             complement(3233..>3854)
                     /locus_tag="VGM24_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY4156336.1"
                     /translation="LLSCTYSSKATKKLYQKALEKKPYDAIIVPGIPFENGKWGRVMK
                     GRIYWSKFLYDHGVTRNIIFSGSAVYSPYVEADIMAAYAVALGIPRDHVFTETRAEHS
                     TENVYYAYRLSQTLGFKKIALASDPSQTRLLRRFTRKKVSAGIDLIPMVEDSLKAMEP
                     EMKDPVIDPAPAYRKDFISLQKREGFFKRLRGTLGKNIDSTAHNNP"
BASE COUNT         1002 a          982 c          944 g          926 t
ORIGIN      
        1 tggtctccat tgaagtgaat tatgaagcaa aagaagattt ggtcctggcg accggaaaga
       61 tctttcatga agaaaccata ttcatgaagt acagcctgga acttattcca atgccggctt
      121 aacggacatt cttcctaata gaaaattcaa actgcgttcc ttccccgggc gtggaatgca
      181 gtccgatctc tcctccctgt ttttcaacca gatttttaac cgttgtcagt ccgaggccgg
      241 tgccccggat gccggcgcgg tcgggaatcc ccaggctggt aaagggctca aagatctttt
      301 gctgatctgc cagggcaatg ccccgcccgt tatcctgcac ttcaaaataa taaaaggact
      361 ccgtttcccg gaaccggata tccaccacgg ttatttcctt gtcgctgtac cggattgcat
      421 tttgcagcag gttgatcagg atctgttcca gtgccacccg gttgccatgc ataaaaacat
      481 cctctgcggg ataattgatt tgggcggaac ggcctgcctt gagcagcagc acgacggatt
      541 cgatgctgga tctcagttca aacggttcgg ccggttcatt caaaacacgc tttccccggt
      601 aaaaagaatg aacggcttcc acgaggttcc tgatcttaaa agacgcccgg ctgagcacat
      661 tcagcaactg atcattgttt tcatcaatcg cgtccccaaa attctcgcgc agcatttctg
      721 cgcttagcac gatgctggac aaaggcgatt ttatatcatg cgatatcaca taggtaaatt
      781 gttccagttc atattttgtt tcatccagat cttctttcag cttttcgata tccagggcgg
      841 cctttcgcca tttcagcagt tgcaggacct gtccggccag catttggaga gactcctgct
      901 gcatctcgga aagttccccg ggcttccggt ccattacgca gagaaggccc aggagccaac
      961 cttccgggtc cacgagcgga actccggcat aaaaaaccag ttccggatct ccggcgatca
     1021 gcggcttgcc ggcaaaccgt tcttctttgc gcgcatcggg aacaataaat attccgggat
     1081 tccgtgcttc ggcaaccaaa gtattattca gcggggtttc ccggatctcc agaccaagca
     1141 ctgatttaaa ccagaggctt tgcgaatcga tcagcagaat ggcagctgct gcagtctggc
     1201 agattgcggc cgccagccgg gtgatcccgt caaatgcctc ttcgggacta gaatccagga
     1261 tccggtattc ctttagtgcg gccaggcgct ttccttcatc atagggcagt gctggattat
     1321 ccatcagctc tttatttttc ctgaattgaa tttgaattcg gtagtgtaaa atacaaaagg
     1381 gcggctacat atcatttaaa gcccggaggc aatgacgggt aaatttttaa ccgccggaac
     1441 atagaggcgg aaaaagtcat tctcccatac ctgccggtac tggtatcttt tcaggaaggt
     1501 ctgcaggtcg ttccgggtat ttttccggat aacggaatca tcgggatcat tccgtggcca
     1561 gttattgtcc aatgtacttc gtttctgcat gatgaccacc gggcagacat gcgtttcctg
     1621 cagggattcc gtcagttccg ttttgaaatg ggcatcatca tatagccaga cccaggaatt
     1681 gtgcatgaag ggacggctgt cggtcagaaa ataatacatc ggaagacagt cataagccag
     1741 tacccaatcg tccggcctca cgtattttga actggcctcc agcaattcat tgatcgtctt
     1801 tgcccgttcg ggcgtggtgc tgatatagga agcgtgctca ttgttgatct cataccgcat
     1861 cctggcgcgg ttgctgcggt cggcatgggg ataaaaataa caaacgctca atatatacgt
     1921 aagcgtaagg aagatgaatc cgttccggag ggaattcatc tggtttgcat tgataacctg
     1981 ctcgtaagta tgccggttgt tctcgctcac tacgatggaa ctgctcaggg aacggatatt
     2041 caacaggtaa tccacggcga tgggcaccat gatccaggcg gcatattttc caacggtcat
     2101 cagcaccaca tgacttccca ccggcaggat cagcagcatg aggcagccaa tggccgaaag
     2161 cagctgcatg tccttgggct gccgtcccgt aatgatgaga aaaccgatga tcaggctcgt
     2221 tccggaatag aataaaaaaa gatagtacca ggaacggccg tctacccagg catggataat
     2281 acagagggcc gtcaggataa ccagtattcc gtatttccca aaaatccaaa agccccggag
     2341 aaaatagatt tccattttca tccttctcca gatggccgca aaactccaga ggccaacgag
     2401 cacggtgatg gaaaccgtca gcccttcgct gtactgcagc aaatacagtt tcaggctatt
     2461 ttgtaaattc cgggagttca cctggttcct gcccgtttgc ctcagtattt cgagattgtt
     2521 aaaaaagaaa acgtcgtgat gcaggtcttt catgaccaga aaaagtccgc aggccatcaa
     2581 caggaaaccc gtcaggaata aaaggcattg aaacaggatc cggtaaaggg gaatgcggtt
     2641 taaatagccg gagaatacaa tcgccaggat caggaaaaag tccagcccgt tgggaagccg
     2701 cgcaaacata ttggccgcaa caaacatccc ggaaataaaa atccggaatt gtttctggcg
     2761 ggtcagcccg gtaaaaagaa accaggcggc gcaaataaaa aacagggcgg aaatatcgcg
     2821 gtagttgaac tccttgatac cgttcccgag aaaaagaaca atcagcagca ggccgagccg
     2881 cagggatgcc gtctgcaggt attttttcag caggttatag cttatggcaa aagtgaaggt
     2941 ggtacagatc acgccggcaa tgcgcaggcc cagcagaccc aggccaggga acagcttcag
     3001 ccagagtccg ccgatgatcc cggtaagcca gtacgtaaaa ttatattgca cggtagaagg
     3061 gtcgctgaag atccgctggt agaaaaccgc tgcaaaaccg gagtccgtaa aatcaagtcc
     3121 ctggaaaata aaaataagct gtaccaggat aaccagcccg aaatacagct tcccattcat
     3181 tcgcccaaaa aaaacctcga taaaactctt ttccatgctc caatacttcg ggttaaggat
     3241 tattatgcgc ggtgctgtca atatttttac ccagggtgcc ccgcagtctt ttgaagaaac
     3301 cctcccgctt ttgcaggctg ataaaatctt tgcggtatgc cggagccggg tctatgaccg
     3361 gatctttcat ttccggttcc atagctttca gactatcttc caccatcggg atcaggtcga
     3421 tccccgcact cactttctta cgggtgaatc gccggagcag ccgggtctgc gaaggatcgg
     3481 aggcgagggc gatcttttta aaacccaggg tctgcgagag ccggtaagca taatatacat
     3541 tctccgtgct gtgctcggcc cgggtctcgg tgaaaacatg atcccttgga atgcccaggg
     3601 ccacggcata ggccgccatg atatccgctt ccacataggg cgagtacacc gccgatccgg
     3661 agaaaataat attgcgggtc actccgtgat cgtataaaaa tttagaccag tagatccgcc
     3721 ctttcatcac cctgccccat ttgccgttct caaaaggaat ccccggaacg atgatcgcat
     3781 cgtatggctt tttttccagc gccttctgat ataatttttt tgtggctttt gaagagtagg
     3841 tacaggataa aagc
//
LOCUS       DASYCU010000642         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4198051, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000642 DASYCU010000000
VERSION     DASYCU010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4198051"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VGM24_12940"
     CDS             <1..664
                     /locus_tag="VGM24_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156337.1"
                     /translation="IPGSGKPYGFRAEGPAGMVCTVVNPSQETVSMNLPATAKISAVL
                     YSDGGYRPVLQGSRLSLGPEQLAVIGFDGYASDKYNLGRDETISIPVTISKINASFTQ
                     TGKNKIETTIGKLPIKNIRILFQQFDSNGFPYRTWGGAPPDGKKMDQLFTIRVSQNSK
                     PVPIHIEYDKMIWSGLSWAAGEISEDDLDFGRPLTIECGSAETKELSLKATVYAVGYS
                     TR"
     gene            639..2069
                     /locus_tag="VGM24_12945"
     CDS             639..2069
                     /locus_tag="VGM24_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY4156338.1"
                     /translation="MQLDTAPVKCIKPILAFLTGIPFLVLPVAAQNNHSFIRRLHPAP
                     LSVLQVDLKVKVSPAPVKACAFLRFYDSLDHELLEYKSAPVSSDTLQETGFYTEAPPY
                     TNYATVSVEPDSGKPAEITVEGFHADPQEGVPEKKFAPLCDLDQYMRPFWKSDTIFNE
                     TVLLYSRNGMAATGRLLFQPDKILSVQRFDLKTTYSEKEDYFVNGRTIGRRPNSKMPF
                     RADTSFNTRDDLAWFNLQSQWVVVTYTHRDKWEGQVPAYKGNSMPRTLAKLRSKSPLR
                     IVGYGMSITRGLDVSSYDTVAPFMPTYLQLFTRALKKQFGYGDIQLYNAGLPGAKADW
                     GASYADQYINPLKPDLVIIDFGMNDFWIYKPEEFSAFIQTIIQKVKRANPEAEFLLLS
                     NMKFDPDYVLDPDKNKAFYLGNMEGYNTGLQKLVVTGITDLDMTTISGYIYSRKKAKD
                     CLANPLHPNDYLARWYAQGMAAIFRK"
     gene            complement(2072..>3757)
                     /locus_tag="VGM24_12950"
     CDS             complement(2072..>3757)
                     /locus_tag="VGM24_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEY4156339.1"
                     /translation="SLDDISREIESLFKLLAGPKESILDKLRILPRLSRFAAWMPRVI
                     SGRGACQEVLMKSPDLNKLPVITCWPKDGGPFITLPVIHTKDPETGIRNVGMYRMQIL
                     DATRTGMHWHKHKVSARHYQAYKKLNRRMPVAVALGGDPVYAYSATAPLPDNVDEYML
                     AGFLRKKKVELVKCLTQPEVEVPADADFVIEGYVDPMEDPIWEGPFGDHTGYYSLADW
                     YPSFHVTAITHKKNPVYPATIVGIPPQEDAWLGKATERIFLAPMKMMMIPEMVDMEMP
                     VEGVFHNLVIAQIKKEFAGHAQKVMSAMWGAGQMMFNKILVLVDENVSITHYKSLAQY
                     VLKNLNPATDISFAQGPMDVLDHSCSKLGFGGKMCIDGTKKFPEERNERYADSGNPDK
                     IPEKELLKAYPEIRAVNTQLLKYQIPCLVVSVAKTRKGQVGELHQAISAQLLPDAPVK
                     LILYIDAMIDPNNLPVALWRFCNNLDPRRDHILFEGKKENKMISCMGLDGTTKTEALD
                     DFHRDWPNIIVADDETISVVDGKWASLGLGPFISSPSRLYRDQMYGPEAVASV"
BASE COUNT          942 a          949 c          925 g          941 t
ORIGIN      
        1 aattcccggt tccggcaaac cctacggttt ccgggcggaa ggaccggccg gcatggtttg
       61 tacggtagtt aatccttccc aggagaccgt atccatgaac cttccggcta ctgctaaaat
      121 ttctgccgta ttatactcgg atggcggata ccggcctgta ttgcagggca gccggctttc
      181 cctggggccg gaacaactgg cggttatcgg atttgatgga tatgcttcgg ataaatataa
      241 tctgggaaga gatgagacca tctcgattcc ggtgaccatt tcaaaaatca acgcttcctt
      301 tacacaaacc ggaaagaata aaattgaaac gacgatcggg aaattaccca taaagaatat
      361 ccggattctg tttcagcaat tcgattcaaa cggatttcct taccgcacct ggggcggcgc
      421 accgccggat ggaaaaaaaa tggaccagct cttcacgatc agggtatcgc aaaatagcaa
      481 gccggttccc atacatatag aatatgataa aatgatatgg agcggcctgt cctgggctgc
      541 cggagaaatc agcgaagatg acctggattt tggcaggccg ctgaccattg aatgcgggtc
      601 cgcggaaaca aaggagctca gcttaaaagc aaccgtatat gcagttggat acagcacccg
      661 ttaaatgtat aaaaccaatc ctggctttcc tgaccggcat tccttttttg gttttgccgg
      721 tcgctgcgca aaataatcat tcgttcattc gccgcctgca tccggcgccc ttgtccgttc
      781 ttcaggttga tctgaaagtg aaagtcagcc cggctccggt aaaagcctgt gccttcctgc
      841 ggttttatga ttcgcttgat catgaactgc tggaatataa aagcgcgccg gtttcttccg
      901 atacccttca ggaaacggga ttttataccg aagccccgcc ctatacaaac tatgctacgg
      961 tttccgtgga accggattcc ggaaaaccgg cagaaataac ggtcgaagga tttcatgccg
     1021 atccgcagga gggcgttccg gaaaagaaat ttgccccctt atgcgatctg gatcaatata
     1081 tgcgcccttt ctggaaatcc gacacgattt ttaatgaaac cgttttactg tacagcagga
     1141 atggaatggc cgccacgggc cggcttcttt ttcagcctga caaaattttg tccgttcaac
     1201 gttttgatct gaagaccacg tattcggaaa aagaagacta tttcgtgaat ggaagaacga
     1261 tcgggcggcg gccaaattca aaaatgccct ttcgcgccga tacttccttt aacacaaggg
     1321 acgacctggc ctggtttaac ctgcagtccc aatgggtggt ggtcacctat acgcacagag
     1381 ataaatggga aggccaggtt cctgcatata aaggaaacag catgccgcgg acgctggcaa
     1441 agctcagatc aaaatctcct ttgcgcatcg tgggttacgg aatgagcatt acccggggac
     1501 tggacgttag tagttacgat accgttgctc cctttatgcc cacctacctg cagctgttta
     1561 ccagggcgct gaaaaaacaa tttggatacg gggatattca actttacaat gccggtcttc
     1621 cgggcgccaa agccgactgg ggcgcgagct acgcggatca gtacatcaat ccgctgaaac
     1681 cggatctggt gatcattgat tttggaatga atgatttctg gatttataaa ccggaggaat
     1741 tcagcgcctt tatccaaacc atcatacaga aagtgaagcg ggccaacccg gaggcggaat
     1801 tcctgctgtt atcgaacatg aaattcgatc ccgattatgt actggacccg gataaaaaca
     1861 aagcatttta tctggggaat atggaagggt ataataccgg gcttcagaaa ttggtggtga
     1921 ccggaatcac ggacctggac atgaccacta tcagcgggta tatttactcc cgaaaaaaag
     1981 ccaaagattg tcttgccaac ccgctccatc cgaacgacta tctggccagg tggtatgcac
     2041 agggcatggc tgctatattc aggaaataat ctcagaccga agccacggcc tccggtccat
     2101 acatttgatc ccggtacaaa cgcgacggag aagaaatgaa cggtcccagt cccagggaag
     2161 cccatttccc atccactaca gatatggttt catcgtccgc cacgatgatg ttcggccagt
     2221 cccggtgaaa atcatccagg gcttctgttt tagtggttcc gtccaggccc atgcaggaaa
     2281 tcattttatt ctcctttttt ccttcaaata agatatgatc gcgcctggga tccaggttat
     2341 tacaaaaacg ccatagagca accggaaggt tgtttggatc gatcatcgca tcaatataca
     2401 ggatcagttt aaccggagcg tcaggcagca gttgggcgga tatggcctga tgcagctcgc
     2461 ccacctgccc cttacgggtc tttgcaaccg aaactacgag gcaggggatc tgatatttaa
     2521 gcaactgcgt gtttaccgcc cggatctccg gatatgcttt caataattct ttttccggaa
     2581 tcttatccgg attgccggaa tccgcatacc gttcgttcct ttcttccgga aattttttgg
     2641 ttccgtcgat gcacattttc cctccgaaac ccagcttgct gcagctgtga tcgaggacgt
     2701 ccataggtcc ctgtgcgaaa gaaatatccg ttgccggatt caggtttttt aaaacatatt
     2761 gggcaaggga tttataatgg gtgattgata cgttttcatc cacgagcacc aggatcttgt
     2821 tgaacatcat ttgtccggcg ccccacatag cgctcattac cttttgcgca tgtccggcaa
     2881 attccttttt tatctgtgca atcaccaggt tatgaaacac gccttccacc ggcatttcca
     2941 tgtccaccat ttccgggatc atcatcattt tcatcggagc cagaaagatc cgttcggtgg
     3001 cttttcccag ccacgcatct tcctgcggag ggatgcccac aatggtagcc ggatagaccg
     3061 gatttttttt gtgcgtaatg gccgtgacat gaaaagacgg ataccagtcg gccagggaat
     3121 aataccccgt atggtctccg aagggtcctt cccagatggg atcttccatg ggatccacat
     3181 agccttcgat cacaaaatcg gcatcagcgg gtacttccac ctcgggttgg gtaaggcatt
     3241 tcaccaattc cacttttttc ttccgcaaaa acccggcaag catatattca tccacgttat
     3301 cgggcaatgg agcggtggcg gaataagcat acaccggatc tccgccaagc gcaacggcga
     3361 cgggcattcg ccggtttaat tttttgtaag cctggtaatg ccgcgcactt actttatgtt
     3421 tgtgccagtg catgccggtc cgggttgcat cgagtatttg catacggtac atgcctacat
     3481 tccggatgcc ggtttcggga tccttggtgt gaatgacggg tagtgtgata aaggggccgc
     3541 catctttggg ccagcaggta atgaccggaa gtttgttcag atccggtgat ttcatcagta
     3601 cttcctggca ggcaccccgg ccactgatca cccggggcat ccaggcagca aaccgggaca
     3661 gccggggcaa tatccggagt ttgtccagga tggattcttt aggtccggcc agcagtttaa
     3721 agagagattc aatctcccgg ctgatatcat ccagact
//
LOCUS       DASYCU010000643         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2896129, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000643 DASYCU010000000
VERSION     DASYCU010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896129"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGM24_12955"
     CDS             <1..572
                     /locus_tag="VGM24_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156340.1"
                     /translation="FRATPITLNKLSQYAADFTEIYNKAWSGHGGMKQLRLEQVSQMF
                     RQMKAVIDPRLIWFVYHLDKPIAMFVNIPDVNQWFKLLNGKFGLAAKLKFLWVKKTRP
                     NKKFTGVAFGIIPEYQAKGVDAFMIVETGKLVNTTHYSEYEMQWIGDFNPKMINLVKA
                     LGDVYQSRQLTTYRYIFDRTREFKRHPIF"
     gene            587..2479
                     /locus_tag="VGM24_12960"
     CDS             587..2479
                     /locus_tag="VGM24_12960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY4156341.1"
                     /translation="MQRKVTGGCLVMDKFIVSARKYRPQNFSTVVGQSHITTTLKNAI
                     RNQQLAHAFLFCGPRGVGKTTCARILAKAINCAHPADDGEACNECHSCISFNEGTSLN
                     IHELDAASNNSVDDIRSLVEQVRIPPQAGKYKVYIIDEVHMLSSFAFNAFLKTLEEPP
                     PYAIFILATTEKHKILPTILSRCQVFDFKRISARDIIEHLEEICQKESILADRSAIEM
                     IAQKSEGCMRDALSIMDKLVSFSNGNLEYADTLEHLNILDAGYYFKLMDAVLGQNLTD
                     ALLLYDEINKKGFEGDLLLEGFTEFIRNLLICKDEKAASLLDVVAGFKEKYHSVAQEI
                     SAGYLISALNVLNDSELRYKAARNKRLHVELALIKLSYLGQALHLLDDGTADKKKAGD
                     AVKPVAFRSLPPLEIKEKRPAALPEKQPATEKTAVVESGRGARLIIEEPVKPVSGLSA
                     SAVSAPPDSRKEVSPAAAPVSPAHKISSPATSPGALSKIRQQVINRKQAGSPDLPKPL
                     DLPLLQESWNQFTRDLKENKNSAAQSFDGAILKILDDQTFEILTNNNLEQRFLEQEKR
                     NLSDLLQQVFNNKNLSFSISIRENANQHIHLEKTLSKRDQYLQMAEQYPLLKELKEKL
                     KLELDY"
     gene            2584..>4266
                     /locus_tag="VGM24_12965"
     CDS             2584..>4266
                     /locus_tag="VGM24_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY4156342.1"
                     /translation="MAGNSTTQVFQTSSSSRWSRFKWSSRLLALFLVLGIIIIVLTMT
                     RVYTPSLPNMLGAKEKEVLLDSSGWLFSKSKIGRQYGGFRKYINEKEAYKAGGYPIPK
                     KFRKKNGIIVQADSSYYSFKKFAAGIRAAFYVNWAPSSFTSLEQNIGRLNLVFPEWFF
                     INPAADTLTTDIDKKALDIMNKSGVKIVPMLTNNFNQVFRGDVVHRILGNQDKRERLI
                     NDILRNLEKYKLDGINIDFEDLQEKKNEVLVSFQKELYEKMHARGLMVTQDVTPFNSD
                     YNFTELAKYNDYVVVMAYDQYTEETQPGPICHQKWIEGAVDDIARRVPSSKLILALAG
                     FGYNWKMDDNNRAVSATSISYQDALTLALSFDGKIIFDNDSYNLHFSYDDDNGVQHQV
                     HFTDAATTFNSMRFAVESGLPGVALWRLGSEDSRMWDFYDHDMSKDSLKNFDFRLFST
                     VKSFSLDETPAYSGEGEVLDVIGGPTSGKIRSELDTSELLISEEKYDSLPSKWVARKY
                     GTKDKKKLVLTFDDGPDPLYTPQILDILSREKVPAAFFLVGINAENNIPIVKR"
BASE COUNT         1173 a         1096 c         1028 g          969 t
ORIGIN      
        1 ccttccgggc aacccctatc acgctgaaca aactatccca atacgccgcg gactttacag
       61 aaatttataa taaggcctgg tccgggcatg gcggcatgaa gcagctccgc ctggaacagg
      121 tttcccaaat gttcaggcaa atgaaagccg tgatcgaccc caggctgatc tggttcgtgt
      181 atcatctgga caaacccatt gcgatgtttg tgaatattcc cgatgtcaac caatggttca
      241 aacttctcaa tggaaaattc ggattagcgg ccaagctaaa attcctctgg gtaaaaaaaa
      301 cgcggccaaa caaaaaattt accggcgttg cttttggaat tattccggaa taccaggcaa
      361 agggggtgga tgcctttatg attgtggaaa ccggaaaact ggtcaataca acccattatt
      421 ccgaatatga aatgcaatgg atcggcgatt tcaacccaaa aatgatcaac ctggtgaaag
      481 ccctgggcga tgtgtatcaa agcaggcagc tcactaccta ccggtatatt tttgaccgca
      541 cccgggaatt taaaaggcat cccatcttct gaaactttct gtattattgc agcgaaaagt
      601 taccggaggt tgcctggtta tggataaatt cattgtttcc gctagaaaat acagaccaca
      661 gaatttttct accgtggtgg gccagtcgca tatcaccacg accctcaaaa atgccatccg
      721 caatcagcag ctcgcgcatg cgtttttatt ttgcggtccg cgcggagtag gcaaaaccac
      781 ctgtgcccgc atcctggcaa aagccatcaa ttgcgcacat ccggccgacg acggggaagc
      841 ctgcaatgaa tgtcattcgt gcatttcctt caatgaaggc acctcgctga atattcacga
      901 gctcgatgcg gccagcaaca attccgtaga cgatatccgt tccctggtgg aacaggttcg
      961 gattcccccg caggccggaa aatacaaagt gtatatcata gatgaagtgc acatgctcag
     1021 ttctttcgcc ttcaatgcct ttttgaaaac actggaagaa ccgccaccct atgccatttt
     1081 catcctggcc acgacagaga aacacaagat ccttcccacg atcctcagcc gttgccaggt
     1141 attcgatttt aaacggatca gcgcccggga catcatcgag cacctggaag agatctgcca
     1201 gaaagaaagc atcctggcgg accggtcggc catcgaaatg atcgcacaaa aaagcgaagg
     1261 ctgcatgcgc gatgcgctga gcatcatgga taagctggtc agtttttcaa acggaaatct
     1321 ggagtatgcc gataccctcg aacacctgaa tatcctggat gccggctatt attttaagtt
     1381 gatggatgcc gtgcttgggc aaaacctgac ggatgccctt ctcctctatg atgagattaa
     1441 caagaaaggc tttgaagggg atctgctgct ggagggtttt actgaattta tccgcaacct
     1501 gctgatctgc aaagatgaaa aagcggccag cctgctggat gtggttgccg gattcaaaga
     1561 aaaatatcat tccgttgcgc aggaaatcag cgccggttac ctgatcagtg cgctgaatgt
     1621 gctgaatgat tctgaactgc ggtataaagc ggcccgcaac aaaagattac atgtcgaact
     1681 ggccctgatc aaattatctt atctcggaca ggcccttcac ctgctggacg acggcacggc
     1741 cgataaaaaa aaagcgggtg atgcggttaa gcccgtggcc ttccgctctc ttcctccgct
     1801 ggagatcaaa gaaaaaagac cggcagccct gccggaaaaa cagccggcaa cagaaaaaac
     1861 agccgtcgtt gaatcaggcc ggggcgcccg gctgataatt gaagagcctg ttaaaccggt
     1921 ctccggttta agtgcatctg ccgtgtccgc cccaccggat tcgcggaagg aggtttcccc
     1981 ggcggcagcc cccgtttccc cggcgcataa aatatcttcc cctgcaacca gtccgggcgc
     2041 cttgtccaaa atccggcaac aggtgattaa ccgcaaacag gcaggaagcc cggaccttcc
     2101 aaaaccactg gatctgccgc ttttgcagga aagctggaat cagtttaccc gggacctgaa
     2161 agaaaataaa aattcggcgg cacagagctt cgacggggcg attcttaaga tactggatga
     2221 tcagacgttt gagatcctca ccaacaataa cctggaacaa cgattccttg aacaggaaaa
     2281 acggaacctg tcggatttgc tgcaacaggt tttcaacaat aaaaatcttt ctttttccat
     2341 ttccatccgg gaaaatgcaa atcagcacat tcacctggaa aagaccctga gcaaacggga
     2401 tcaatacctg cagatggcag agcaataccc gctgctgaaa gaacttaagg aaaagctgaa
     2461 actggaactg gattattaaa ccggtagcgc aaaaatcatt ttcacccatt tgctaaaaat
     2521 cctataattt gacctgcctg atttggctgg cccgccgttt cgcgggaact ttatcttctt
     2581 cttatggccg gaaattctac gactcaggtt tttcaaacca gttcatcttc ccgctggtcc
     2641 cgttttaagt ggagcagccg tctgctagcc ctcttcctgg tattgggaat aatcatcatc
     2701 gtcctcacca tgacccgcgt gtatacgccg tccctgccca atatgcttgg cgcaaaagaa
     2761 aaggaggttc tgctggacag ttcgggatgg cttttcagca aaagcaagat tggccggcaa
     2821 tatggcggct tccggaaata catcaatgaa aaagaagcct ataaggcagg cggttacccg
     2881 atcccgaaaa aattccggaa aaagaacggg atcatcgtac aggccgattc cagttactat
     2941 tccttcaaga aattcgcagc gggcatccgg gccgcttttt acgtgaactg ggccccctct
     3001 tccttcactt ccctggaaca gaatataggc cggctcaacc ttgtgtttcc ggaatggttc
     3061 tttatcaatc ccgcagccga tacgctcacg accgacatcg acaaaaaagc gctcgatatc
     3121 atgaataaat cgggcgtaaa gatcgtaccc atgctcacca ataatttcaa tcaggtgttc
     3181 cggggcgatg tggtgcaccg catactcggc aatcaggata aaagagaacg gctgatcaac
     3241 gatatccttc gcaacctgga gaagtacaaa ctggacggga tcaacataga cttcgaagat
     3301 ctgcaggaaa aaaagaatga agtgctggtg tctttccaga aagagctcta cgaaaaaatg
     3361 cacgcccggg gtctgatggt tacacaggat gttactccct ttaacagcga ttataatttt
     3421 acggaactgg ccaaatacaa cgactacgtg gtggtaatgg cctatgacca gtacacggag
     3481 gaaacgcaac ccgggcccat ctgccaccag aaatggatcg agggggcagt ggatgatata
     3541 gcgagaaggg ttccctcttc caaactgatc ctggccctcg ccggtttcgg gtataactgg
     3601 aaaatggatg ataacaacag agcggtgtct gccacctcca tcagctacca ggatgccctc
     3661 accctggccc tgagttttga cggcaagatc atttttgata atgattctta caatcttcat
     3721 ttcagttacg acgatgacaa cggcgtacag caccaggtgc atttcacgga tgccgccacc
     3781 actttcaatt ccatgcgttt tgccgtggaa tccggcctgc ccggagtggc cctttggcgg
     3841 ctgggcagcg aggattcgcg catgtgggat ttttatgacc acgacatgag taaggacagc
     3901 ctgaagaatt tcgattttcg gttgttctcg accgttaagt cattcagcct ggacgaaacg
     3961 ccggcttatt cgggagaagg cgaagtgctc gatgtgatcg gcggccccac cagcggaaag
     4021 atccggtctg aactggatac gagtgaactc ctgatcagtg aagaaaaata cgacagcctg
     4081 ccctccaaat gggttgcccg gaaatacggc acaaaggata agaaaaagct ggtgcttacc
     4141 ttcgacgacg ggcccgatcc cctgtatacg ccccagatac tggatatcct cagccgcgaa
     4201 aaagtgccgg ccgctttttt cctggtcgga ataaatgctg aaaataatat cccgatcgtg
     4261 aagcgc
//
LOCUS       DASYCU010000644         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4921578, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000644 DASYCU010000000
VERSION     DASYCU010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4921578"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VGM24_12970"
     CDS             complement(<1..824)
                     /locus_tag="VGM24_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156343.1"
                     /translation="MKQILTMILTLSLASQLQAASIYNISSNSSWSSTIPSYCGNCTI
                     NIASGATLNLNNSSMCDNCKISGGAIKITGGFSFQSSTISNTSITATSSFTLNNTNSF
                     SDVTAVISGSSSFTANGALTLTNSSFTFKNTASFTSNGQLNIDNSQLDLNDNSHFTSN
                     SSTVNLKNGSTLVAGDGSKSSKAYMIFYGQLNLVDAASMLIISNSNNYYYSWNTYKSQ
                     SNGTSYTTTSNYQAYYYGGAVFSSQGPLPISILPITIDGFKAAEINPHSVKLTWTLT"
     gene            complement(956..2470)
                     /locus_tag="VGM24_12975"
     CDS             complement(956..2470)
                     /locus_tag="VGM24_12975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY4156344.1"
                     /translation="MATLNILPGTQAPKSDWKTLAWLVSGIIIIRLLFIGIMGMMPQD
                     AYYDFYAQHLALSYYDHPPVIAYLLRGFTTVFGKKVFVLKLADSLVTAFSVLAFYSLA
                     KRFLSIPRARTGLILLLSTLMISILSLVSTPDVPLILCWTVTLIFLHNAVFYEKKSYW
                     IWAGILTGLSFDSKYTAVFLIFGLVGFLLISRKYRHNLFSGWFLLFLVSFAVTVLPVY
                     IWNARNGFASFKFQSEARVNSMEGFHISITDFGGVIGHQSALLSPILLFSFFYLLYRL
                     IRKYKWKFALIPDGQLFLLSFFVPLFLGFLFISFFYWVKINWMMPAYISGIIWLCRYW
                     KKKWIRIQLVFSLILHLAFAAEVIFYIVPVRSDDTWFGWKDFARQVSLVRTEHPAAFI
                     FSADDYKTSAVLNFFLDEMVYSKNVVGERALQFDFIGTDLNQLQGRDALFIDSNPRFS
                     NLENESDKIPSFYNRYFDRIIPLKPILVKRNGRVERKFSVFLCRNYHPAAIPSR"
     gene            complement(2473..>4029)
                     /locus_tag="VGM24_12980"
     CDS             complement(2473..>4029)
                     /locus_tag="VGM24_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY4156345.1"
                     /translation="EGAESLSATGKGTICNMGAEIGATCSIFTYDSKMSDYLKATGRA
                     EVAALADGIREQLRPDPEVYASPADYYDQLIEINLDELEPHVNGPFTPDLAWPISKFA
                     EAVKANQWPDKLEVALIGSCTNSSYEDISRSASIASQAIQKKLKTRAEFTITPGSEQV
                     RFTIEKDGFLKTFEKIGGVVLANACGPCIGQWARHIDDPSRKNSIITSFNRNFARRND
                     GLASTHAFVASPEIVTAFAIAGDLKFNPLKDTLRNEQGVDVMLDEPTGYELPPKGFSV
                     DDPGYQAAAKDGSGVQVVVKPDSQRLQLLEPFKAWEGADLKGLRLLIKAKGKCTTDHI
                     SMAGPWLKFRGHLDNISNNMLIGAVNFFNEQTDLVKNGLTGEYGPVPQTARAYKAAGV
                     GSIVVGDENYGEGSSREHAAMEPRHLGVRAILVRSFARIHETNLKKQGMLALTFSNKS
                     DYDKVLEDDLIDINGLVNFREGKPLTVILHHGNGSTEEIPVNHTYNEQQIEWFKAGGA
                     LNVIRAEFAR"
BASE COUNT         1050 a          914 c         1039 g         1026 t
ORIGIN      
        1 gtcagggtcc aggtaagttt aacggaatgg ggattgattt cagctgcttt aaaaccgtcg
       61 atggttatcg gcagaatgga aatgggcaga ggaccctggc tgctgaaaac ggcgcctccg
      121 taataataag cctggtaatt actggtggtg gtataactcg ttccgttgct ctgtgactta
      181 tacgtattcc agctgtagta atagttattg ctgttggata taatgagcat tgatgcggca
      241 tcaaccaggt tcagctgacc gtagaagatc atataagcct tgctgctttt actgccatcc
      301 cctgctacaa gggtacttcc gtttttcaga tttacggtgg aagagttgga agtaaaatgg
      361 gaattatcgt taagatctaa ctggctgtta tcaatgttca gctgaccgtt actggtaaag
      421 gaagcggtat tcttaaaggt aaagcttgaa ttggttaagg ttaaagcccc gtttgccgta
      481 aagctggaag aaccagagat gaccgcagtc acatcactaa agctgttcgt gttattcagc
      541 gtaaaggaag aagttgcggt aatgctggta ttgctgatgg tagatgactg aaaggaaaac
      601 cctccggtta tttttattgc tccgccgctg atcttgcaat tatcacacat actgctgttg
      661 ttcaggttaa gtgttgcacc ggaagcaata ttgatggtac aattgccgca ataagaaggt
      721 atggtagaac tccatgagct gttggatgaa atattataga tacttgccgc ctgtaattgg
      781 cttgctaaac tcagggtcaa aatcattgtc aggatctgtt tcatatttat tgaattgaaa
      841 caggagaggc caatcgtata ccaacttgta acttatttat tattaatgat atacgtatta
      901 tgtatttttg cgtttattcc tgatttggga aactattcca aattagtaag cgagttcagc
      961 gcgaaggaat ggcggccggg tggtaattcc ggcacaggaa tacggagaat ttcctttcca
     1021 cccgtccgtt tcttttgacc aggattggct tcaggggaat aatccggtcg aaatatctgt
     1081 tatagaagga agggatttta tccgattcat tttcgaggtt cgaaaaccgg gggttggagt
     1141 ctatgaaaag ggcatcccgg ccttgaagct ggttcaggtc ggttccgata aaatcaaact
     1201 gaagcgcccg ttcccccacg acattttttg aatagaccat ttcgtccaga aagaaattga
     1261 gtacggccga ggtcttgtaa tcatccgccg aaaatataaa agctgcggga tgttccgttc
     1321 ttaccaggct gacctgccgg gcaaaatcct tccagccgaa ccaggtatcg tcagaccgga
     1381 cgggaacaat ataaaaaatt acttctgccg caaacgccag atggagaata agtgaaaaga
     1441 ccagctggat ccgtatccat ttttttttcc aataccggca gagccagata atcccggaaa
     1501 tataggcggg catcatccag ttgattttca cccagtaaaa aaaagaaatg aataaaaaac
     1561 ccagaaaaag cggcacaaaa aaacttagca ggaataactg accgtccggg atgagcgcga
     1621 atttccattt atattttcgg atcaggcggt atagcaaata aaagaaagag aataacagga
     1681 taggcgagag gagggccgac tggtgtccga tcaccccacc gaaatcggtg atgctgatat
     1741 ggaatccttc catggaattt acccgggcct ccgactggaa tttaaaagag gcaaacccgt
     1801 tgcgggcatt ccagatatac accggcagaa cggtaaccgc aaaactgacc aggaatagta
     1861 agaaccatcc ggagaacaga ttatgccggt atttgcggga gatcagcagg aacccgacca
     1921 gaccgaagat caggaatacg gcggtgtatt tgctgtcgaa gctgagcccg gtcaggatgc
     1981 ctgcccagat ccaataggat ttcttttcat aaaaaacggc gttgtgtaaa aagatcagcg
     2041 tgaccgtcca gcaaaggatt aagggaacgt ccggggtgga gaccagggag agaatgctga
     2101 tcatcaaggt agacaacagg aggatcagac cggtccgggc cctgggtatg ctaaggaatc
     2161 ttttggcaag ggagtaaaag gccagaacgc taaacgcggt gaccaggctg tcagccagtt
     2221 tcagcacgaa cactttcttt ccaaaaacgg tggtgaagcc tctcaggaga taggcgatca
     2281 ccggaggatg atcgtaatag gagagggcca ggtgctgcgc ataaaaatca taatatgcat
     2341 cctggggcat catgcccatg atcccgataa acagcaaacg aataatgata atgcccgaca
     2401 ccagccaggc cagggttttc caatcggatt tgggtgcctg ggttcccggc agaatattca
     2461 gagttgccat gattagcggg caaattcggc ccggatcaca ttcagcgcgc caccggcttt
     2521 aaaccattct atctgttgtt cattgtacgt gtgattcacg ggaatctctt ccgttgaacc
     2581 gtttccatga tgcaggataa cggtaagcgg ctttccttcc cggaaattga ccagcccgtt
     2641 aatatcgatc agatcatctt cgagaacttt atcataatcc gatttgttgc tgaaggtcag
     2701 cgccagcatc ccctgttttt tcaggttggt ttcgtggatg cgggcaaagg accggaccag
     2761 gatggcacgc accccgagat gccgcggttc catggccgca tgttcgcggg aagatccttc
     2821 tccataattt tcatcaccca ccacgatact tcccactccg gctgccttat acgcccgggc
     2881 tgtttgcggt acgggcccgt attcgccggt cagtccgttc ttgacgagat ccgtttgttc
     2941 gttgaaaaaa ttcactgccc cgatcagcat attgttggaa atattatcga gatgtccacg
     3001 gaatttcagc cagggtccgg ccatggaaat atgatccgtg gtacatttcc ctttggcttt
     3061 gatgagcagg cggagccctt tcaggtccgc gccctcccag gctttgaagg gttcgagcag
     3121 ttgcagtcgc tgggaatccg gtttcacaac cacctggacc ccgcttccat cttttgcagc
     3181 ggcctggtat cccggatcgt cgacagagaa tccttttggc ggtaattcgt atccggttgg
     3241 ttcatccagc ataacatcca cgccctgctc attgcgaagg gtatctttta gcggattaaa
     3301 tttcagatcg cccgcaatgg cgaaggcggt aacgatttcc ggagaagcca caaaagcatg
     3361 cgttgaggcc agtccgtcgt tgcgccgggc aaagtttctg ttgaaagaag tgatgatcga
     3421 atttttacgg gagggatcat caatatgcct tgcccactgg ccgatgcagg ggccgcaggc
     3481 attggccagc accaccccgc cgattttttc gaaagttttc agaaagccgt ctttttcaat
     3541 ggtaaaacga acctgttcgc ttccgggggt gatggtaaat tcggccctgg tctttaattt
     3601 tttctgaatg gcctgactgg caatggaagc ggagcggctg atgtcttcgt aggaagaatt
     3661 ggtgcaggaa ccgatgaggg cgacttccag tttatccggc cattgatttg cttttacggc
     3721 ttctgcaaat tttgagatgg gccaggcaag atcaggggtg aacgggccat tcacatgcgg
     3781 ctccagttca tccaggttga tttcaattaa ctgatcataa tagtcagcgg gactggcata
     3841 aacttccgga tccgggcgaa gctgttcgcg gatgccatct gctaaagcgg ccacttctgc
     3901 gcggcctgtc gctttcaggt aatccgacat tttggaatcg taggtgaaga tggaacaggt
     3961 tgccccgatc tccgcgccca tattacagat ggtccctttt ccggttgcgc tcaggctttc
     4021 agcgccttc
//
LOCUS       DASYCU010000645         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3187924, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000645 DASYCU010000000
VERSION     DASYCU010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3187924"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VGM24_12985"
     CDS             <1..980
                     /locus_tag="VGM24_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156346.1"
                     /translation="EKLFSHVRQPSLPIITDDSILNRFFPHNSVPHHVWIDKEGSVQY
                     ITDGYNAVADNISKFLEGRDLALHYKNESPDFHPDSSLLEETAGSLYKDMIGYSIALH
                     KIEEYGGNRIAFSTDSIQHTTGFKLLNIPLLDAYKMAWAKSIYTEGGEFRKNNRVVLL
                     TRDSNNFKYPARVSQIPAWESQNIISYESKWPLKSQCIAYLFLQKDLNELFPYQAGVE
                     ERETPYYALLRTKLVKRIKNTNPRFVYNDTLYDLQNMPLSNLVQSLNDLECFEKRPVI
                     SPYPSDLKISLTLHGSLSDFKTLQAELKKNGFQLRKKRGKLKMLVVRDK"
     gene            complement(1029..1322)
                     /locus_tag="VGM24_12990"
     CDS             complement(1029..1322)
                     /locus_tag="VGM24_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156347.1"
                     /translation="MKKYLGIFIVLLAVAASAFTGTHKKAVFSGNSYTYNLYGQPHQD
                     DGVNLNNPVNYTLVGTGPLSCSGTAHRCGVENATDDGTNHPDFNLPYTIKKRN"
     gene            complement(1422..1883)
                     /locus_tag="VGM24_12995"
     CDS             complement(1422..1883)
                     /locus_tag="VGM24_12995"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY4156348.1"
                     /translation="MRKQKNNKFRLPDIVTLALLFLFVYTASGKLSDHSAFYSKLILF
                     PFIKSYAFFISWGIPLTELLIATLLILPSKRITGLYGSVFILILFSVYILLMIRNGGR
                     LPCSCGGVIEYFSWTQHFLFNLFFIALTVIAIRLERKRPLPEAGKTNNRPV"
     gene            complement(1999..2979)
                     /locus_tag="VGM24_13000"
     CDS             complement(1999..2979)
                     /locus_tag="VGM24_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY4156349.1"
                     /translation="MYPDFNHPKLNHFRLADSLPPNYRGMVLRAGQVTAADANQGIMV
                     LQDIIQPEFQIHFHLYDLLKPLFIPARQEKTFLVAFLALRNSIQYFIGGLGKFQLRQG
                     EFALLHSPEQEGILRIEKPGIYQSLEVGWSETLVQQAFPYFPGLQSGLDGKDPKHPFY
                     LYPPGRVAGSKSLDIVREMLKAPYDGPVSLLYFEHKVREYLLSLLTEAGKTAEVRLHL
                     TPEEKERVIVLAEKLRAGPDRKFPIAELARETGMNEMKLKMAFKELFGSGIFEYHLEI
                     RMKEAHRLLEETGLNTKAIAAQVGYELTTSFITKFREYFGYPPSEVRRNS"
     gene            3238..>3457
                     /locus_tag="VGM24_13005"
     CDS             3238..>3457
                     /locus_tag="VGM24_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY4156350.1"
                     /translation="MKKKPAKSKIDLFIISRVKERRENLLKTQEDIAVHLDVSTGYIG
                     HIESPNFRAKYNTGHLNELAKLLKCSPRD"
BASE COUNT          981 a          773 c          779 g          924 t
ORIGIN      
        1 tggaaaaact cttttcccat gtccggcaac ccagcctgcc cattattacg gatgacagca
       61 ttttaaaccg gtttttcccc cacaattcgg ttccccatca tgtgtggata gataaggaag
      121 gttcggttca atatatcacg gatggttata atgccgttgc cgataatatc agcaaatttc
      181 tggaaggccg ggatctggcc ctgcattata aaaacgagtc tcctgatttt catccggatt
      241 cctccctgct ggaagaaact gccggaagct tatataaaga catgatcggt tattccatcg
      301 ccctgcataa aatcgaagag tatggcggaa atcgcatcgc tttcagcaca gacagtattc
      361 agcataccac cgggtttaaa ctattgaata ttcccctgct ggatgcctac aaaatggcct
      421 gggcgaaatc catttatacc gaagggggtg agttccggaa aaataaccgg gttgttctgt
      481 tgaccaggga cagcaataat ttcaaatatc ccgcccgggt gagccagata ccggcatggg
      541 aatctcaaaa cataatttcc tatgaatcaa aatggccgct gaagagtcaa tgcatcgcct
      601 atttatttct tcagaaagac cttaatgagc tatttccgta ccaggcgggt gttgaagaaa
      661 gggaaacccc gtactatgcg cttctccgga ctaagctggt aaaaagaata aaaaacacga
      721 acccccgatt tgtttacaac gatacccttt acgatctgca aaatatgcct ttgagcaacc
      781 tggtccaatc cttaaacgat cttgaatgtt tcgaaaaaag gccggtaatc agtccatatc
      841 cgtcagattt aaagattagt ctgaccctgc acggttcatt gtcagatttc aaaacacttc
      901 aggcggaact aaaaaagaac ggatttcagt tgcgaaaaaa aagggggaaa ttaaaaatgc
      961 tggtggtccg ggataaatga ataagaacta atatggcatt tgatttatgc catattagtt
     1021 taccggatct aattcctttt tttaatcgta taaggaagat taaaatcagg atgattggtc
     1081 ccgtcatcgg tggcattttc cacaccgcac cggtgtgccg tcccactgca ggaaagcggg
     1141 ccggttccaa ccagcgtata attgaccgga ttgttcagat tcaccccatc atcctggtgc
     1201 ggttggccat acaggttata ggtatagctg tttccgctaa agaccgcttt tttgtgcgtg
     1261 ccggtaaatg cgcttgcagc aaccgcaagc agaacgatga aaatacccag atactttttc
     1321 atgattgaac gttttagatt tgtaatcgct tactttttta tacaggtttt cagcatcatg
     1381 cctgcttaat tgcaattaat aagttatatc cattgaatga ttcagaccgg tcggttattc
     1441 gttttgccgg cttccggtaa cggcctcttt ctttccagac ggatagctat tacggtcagg
     1501 gcaataaaaa agagattgaa cagaaagtgc tgggtccagc taaaatattc tatgacgccg
     1561 ccacaggagc agggaagcct gcctccgttc cggatcatga gcaggatgta tacggaaaac
     1621 aaaatcagaa taaacacgga tccgtataag cccgttatcc tttttgatgg aagaatcagc
     1681 aaggtagcta tcaggagctc agtaagagga attccccagg aaataaagaa agcataactt
     1741 tttatgaaag gaaacaagat cagttttgaa taaaaagcac tgtggtccga tagtttaccg
     1801 gaggcggtat acacaaaaag aaagagcagg gccagcgtta cgatatccgg caatctgaat
     1861 ttattgtttt tttgctttct catttgtacc gggtgggttc cggacaaaga tggggcgggg
     1921 ggccggcggc ttccaaataa atggtattgg caattggaag tttgttgttg gcaaatattt
     1981 cggaccggag gacaactatt aactgttccg cctcacttca ctcggcgggt aaccaaaata
     2041 ttcccggaac ttggtgataa aactggtcgt gagttcatag ccgacctgcg cggcaatcgc
     2101 tttggtattg agcccggttt cttccaacag acggtgtgcc tctttcatcc tgatctccag
     2161 atggtattcg aaaattccgg atccgaataa ttccttaaat gccattttta atttcatctc
     2221 attcatcccg gtctccctcg cgagttcggc aatgggaaat ttccggtccg ggccggcgcg
     2281 caatttttca gccaggacaa taaccctttc cttttcttcc ggcgtgagat gaagcctgac
     2341 ctcagcggtt tttccggctt ctgtcaaaag ggataataaa tattccctga ccttatgctc
     2401 aaaatacaaa agggaaacgg gaccgtcata gggcgctttc agcatctccc gcacaatatc
     2461 cagcgacttg gatccggcaa cccgccccgg aggatagaga taaaaggggt gctttggatc
     2521 tttgccgtcg aggccggact gcaggccggg aaaataagga aatgcctgct gcaccagggt
     2581 ttctgaccag cccacttcca ggctttgata gataccgggc ttttcgatcc gcagaatgcc
     2641 ttcctgctcc gggctgtgta atagcgcaaa ttctccctgg cgcaactgaa attttcccaa
     2701 tccgccgatg aagtattgta tggaattcct taaggcgaga aaagcgacca ggaatgtctt
     2761 ctcctgccgg gcgggtataa ataagggttt caacagatca tataaatgaa aatggatttg
     2821 aaactcaggc tgaatgatat cctgtaaaac catgattccc tgatttgcat cagcagccgt
     2881 tacctggccg gcccgcaaaa ccattccccg gtaattcggc ggaagcgaat ccgctaaccg
     2941 gaaatggttt agtttggggt ggttgaagtc gggatacata aaatgccctt cgggataaac
     3001 aggatttgat aaagcaatga gtaaaatact taattcttcc agaaaagatt agggttattt
     3061 cacggctgac tcctgacttt atttccttat tcaggaaaat aacttttccg gtggcctttt
     3121 taaaaacctt tgtcgttcta agtgtattta cttaaaggaa ttgttttccc ctgcagaatt
     3181 ctcctatacc agtatagtac gttaaaataa attcttaggc ttccttgcaa cctgagaatg
     3241 aagaagaaac ctgcaaaatc taaaattgac ctgttcatta tctcccgggt aaaagaacgg
     3301 agagagaacc tgcttaagac ccaggaggat attgccgtac atctggatgt ttccaccggt
     3361 tatatcggac atatcgaaag cccgaacttc agagctaaat ataataccgg acacctgaat
     3421 gagctggcga aattgcttaa atgctcaccc agggatt
//
LOCUS       DASYCU010000646         3785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_286729, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000646 DASYCU010000000
VERSION     DASYCU010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286729"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..2829
                     /locus_tag="VGM24_13010"
     CDS             103..2829
                     /locus_tag="VGM24_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEY4156351.1"
                     /translation="MKKGFSVLLLCFSIFLLQAQVSVTDLLCENRENPLGVDEQAPRF
                     TWQLQSAGRNISQSSYEIRIAMSAADLNRGKNLAWSSSKVLSPASVRVRYEGKKLEPG
                     RNYYWEVRVWDAAGKPSAWSPVAYWQTGLLDPSHWTAKWIEAGFAEDSVTRPSPMFRR
                     EFNAVKKISSATLFITAHGLYEAFINGKKTGDAFLTPGFTSYNKRLQYQAYDVSSLIH
                     PGKNVIGALLGSGWYRGHLAWSGHKDSYGNRVALLAQLEIRYTDGSRETVVSDGKWKS
                     STSDILSSEIYNGETIDARKQQKGWMEPGFNDESWPGVYLRDYPVSQIIATENEMIKK
                     HETFKPVRIFKTPEGDQVIDFGQNLVGWVELRVNGKSGDQVRISHAEVLDKQGNFYTE
                     NLRAAKQQDTYILNVEGEEHFEPHFTFQGFRFIKIEGFPGEIKPENFTAVALYSDMPE
                     TGTFSSSNPLINQLQHNIQWGQKGNFLDIPTDCPQRDERLGWTGDAQVFSRTASFNMG
                     VNNFFSKWLKDVRADQNPDGGVPFVVPNVLGADAASSAGWADVATIIPWNMYLAYGDT
                     SLLANQYSSMKAWVNYMKDHSENDLWNKGFHFGDWLFYRPEDDNDGRSAITDKYLIAQ
                     CFYAHSTQLLINSAKVLGFKEDAASYTDLLDKIKKAYLTEYVTPSGRLVSGTQTAYVL
                     ALQFDMLPESLRPQAVERLVENIRSYDDHLTTGFLGTPYLCAVLTRFGHPEIAYQLLL
                     QETYPSWLYPVKMGATTIWERWDGEKPDSSFETPGMNSFNHYAYGAIGDFMYRDIAGI
                     DTYDSFPGYKKIRIKPHPGGNFSYVNASLKTYYGKVSANWKISGNEFILDAEIPANTR
                     AGIYLPTSDAGSVTMNGEPVSGKNGLNLKTTADGYLFFETGSGAYHFTCTRK"
     gene            complement(2833..>3785)
                     /locus_tag="VGM24_13015"
     CDS             complement(2833..>3785)
                     /locus_tag="VGM24_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY4156352.1"
                     /translation="NNSIKNGTLKKGDRLLSINELSNEFFLSRDTVQKAYDLLEKKGV
                     ILPVRGKGFYINRTDIHTAYRVLLLFNKISNYKKQIYNSFIQTIGNNAVVDLKIHYSN
                     VRVFNNLLENHLNEYDYYVIMPHFYEKAEEAYATIRRIPPKKLLILDKDLSNLNTGYA
                     AVYQDFKNDTRDALQTGLPLLKKYNRLVLVFPRMVAYPKEITTSFINFCMEFNLKYDI
                     IPEINPGQEIRSKEAYIVIEETDLVNLIKICRSKKWAVGKEVGIISFNETPLKEILLD
                     GITVLSTDHAKMGETAARLILENKKEKIKNPFSLLIRKSL"
BASE COUNT          954 a          870 c          982 g          979 t
ORIGIN      
        1 gctttgctga accggtatgc tgcggtaatt ttaccggact ggccagcaca gtggatctgg
       61 tgaaatgctt caacttcgaa ccggtctttt caaattgaac atatgaaaaa aggattttcc
      121 gttttactgc tttgtttttc cattttctta ttgcaggcgc aggtttctgt aaccgacctt
      181 ctgtgtgaaa acagggagaa ccccctgggt gtggatgaac aggctccccg tttcacctgg
      241 cagctgcagt ctgccggcag aaatattagc cagtcatcat atgagatccg catagcgatg
      301 tcggctgccg atctgaaccg tgggaaaaac ctggcctgga gcagcagtaa agttttgtcg
      361 ccagcgtctg tccgtgtacg gtatgagggc aaaaagctgg aacccggaag aaactattac
      421 tgggaagtcc gggtgtggga tgcagcgggt aaaccatccg cctggtctcc ggtggcttac
      481 tggcagaccg gtttactgga tccgtcccac tggacggcaa aatggattga agccggattt
      541 gcagaagatt cggtaacccg gcccagtccg atgttccggc gggaattcaa tgccgtgaaa
      601 aagatttcat cagcgacgct gtttattacg gcccatggat tgtacgaagc ctttatcaat
      661 ggcaaaaaaa caggggatgc cttcctgaca ccgggcttca cgagttataa caaaaggctt
      721 caatatcagg cttacgatgt ttcctctctt attcatccgg gcaaaaatgt gatcggcgcc
      781 ctgctgggct ccggatggta cagggggcat cttgcctgga gcggtcataa ggattcttac
      841 ggaaaccggg tggccctgct ggcgcagttg gaaatccgct atactgacgg cagccgggaa
      901 accgttgttt ccgacggaaa atggaagagt tccaccagcg acatcctcag ttctgaaata
      961 tacaacggag aaacaattga tgccagaaaa cagcaaaagg gatggatgga accggggttc
     1021 aatgatgaaa gctggccggg ggtttacctg agggattacc cggtaagcca gattatcgcc
     1081 accgaaaatg aaatgatcaa aaagcatgaa acctttaaac cggtaaggat cttcaagacg
     1141 ccggaaggag accaggtgat cgacttcggg caaaacctgg tgggatgggt tgagctgcgg
     1201 gtaaatggga agagcggcga ccaggttcgc atatctcatg cggaagtatt ggataagcag
     1261 ggaaattttt atacggagaa cctgcgcgcg gcaaaacaac aggacaccta tattctcaat
     1321 gtagaaggag aggaacattt tgaacctcat tttacttttc agggattccg gttcattaaa
     1381 atagaaggtt ttccgggaga gatcaagccg gaaaatttta ctgcggtcgc gctctattca
     1441 gatatgccgg aaacgggaac tttctccagc tccaatccac tcatcaacca gctccagcac
     1501 aatattcaat ggggacagaa agggaatttc ctggatatcc cgacagactg cccccagcgg
     1561 gatgagcgtt tgggatggac cggcgacgcg caagtatttt cccggacggc atcttttaac
     1621 atgggagtca ataacttttt ttccaaatgg ctgaaggacg ttcgtgccga ccagaacccg
     1681 gacggcggag ttccgttcgt cgtgcccaat gtactgggtg cggatgcagc ttcctctgcg
     1741 ggatgggcgg atgtggccac gatcattccc tggaatatgt atctcgcgta cggggacacc
     1801 tccctgctgg cgaatcagta cagctcgatg aaggcctggg tgaattacat gaaagaccat
     1861 agcgaaaatg atctatggaa caagggattt cattttggcg actggctgtt ttaccgcccg
     1921 gaagatgata atgacggccg gtccgccatc accgataaat acctgatcgc tcaatgcttt
     1981 tacgcacatt ccacccagct tttgatcaat tcggcaaagg tgctgggatt taaggaagat
     2041 gcagcttctt acaccgatct gctggacaaa attaaaaagg cctatttgac cgaatatgtt
     2101 actccttccg gaagactggt atccggaacc cagactgcct atgtactggc attgcaattt
     2161 gacatgctgc cggaatccct gcgtccacag gcggtggaac ggctggttga aaatatccgc
     2221 tcttacgatg accacctgac taccggtttt ttgggaactc cctatttatg tgccgtgctg
     2281 acccggtttg gtcatccgga gatcgcctat caattattat tgcaggagac ctatccttcc
     2341 tggttgtatc cggtaaagat gggagctact acgatttggg aacgctggga cggagaaaag
     2401 cccgacagca gttttgaaac cccgggaatg aattccttca accattacgc ttacggcgcg
     2461 atcggagatt ttatgtaccg ggatatagcg ggcattgata cgtatgattc tttcccggga
     2521 tataaaaaga tccggatcaa accgcatccc ggaggaaatt tcagctatgt gaatgccagc
     2581 ctgaaaacct attacggaaa ggtatccgca aactggaaga tcagcgggaa cgaatttatc
     2641 ctggatgcgg aaattccggc taatacccgg gccggtattt atctgcccac ttcagacgcg
     2701 ggatccgtca cgatgaacgg ggagcctgta tccggaaaga atggcctgaa tttgaaaacg
     2761 acggcggacg ggtacctgtt ttttgaaacc ggttccggcg cgtatcattt tacctgtacc
     2821 cggaaatagc ggtcacaagg atttccggat caggagactg aacgggtttt tgattttttc
     2881 tttcttattt tccaggatca gcctggccgc ggtttccccc attttggcat gatcggtgga
     2941 gagtacggta atgccatcca gcaggatttc tttcaacggg gtttcgttaa aggaaatgat
     3001 gccgacctct ttgccgactg cccatttttt gctccggcag atcttgatca ggttaaccag
     3061 gtcggtttct tcgataacaa tataagcttc tttggatctg atctcctggc cgggatttat
     3121 ttcgggaatg atatcgtatt tcaggttaaa ctccatgcag aaattgataa agctggtcgt
     3181 gatctccttg ggataggcaa ccatccgggg aaataccagc accagtctgt tatatttttt
     3241 cagcagggga agcccggttt gcaaggcgtc ccgcgtatca tttttaaaat cctggtaaac
     3301 ggccgcgtat cccgtattca ggttggaaag gtccttgtcg aggatcagta gtttttttgg
     3361 cgggatccgg cggatggtgg catatgcttc ttccgctttt tcataaaaat gcggcatgat
     3421 cacgtaataa tcatattcgt tcagatggtt ttccagcagg ttgttgaaga cccggacatt
     3481 ggaataatgg attttcaggt caaccaccgc attattgccg atggtctgaa taaatgaatt
     3541 gtagatctgt tttttataat tgctgatctt attaaatagc aataataccc ggtaagccgt
     3601 gtgaatgtcc gtccggttga tatagaatcc ctttcccctt accggcagga tcacgccttt
     3661 tttctccagc aggtcatagg ctttctgaac cgtatccctc gacagaaaaa attcgttgct
     3721 cagctcattg atggaaagca ggcgatcccc ttttttcaac gttccgttct ttatggaatt
     3781 gttga
//
LOCUS       DASYCU010000647         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4492432, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000647 DASYCU010000000
VERSION     DASYCU010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4492432"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGM24_13020"
     CDS             <1..210
                     /locus_tag="VGM24_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156353.1"
                     /translation="FIDEDVPGGAAAYMFNKVMEEQGGFRWLDVSPRTLTAKAHRPAY
                     ASDGDYFSKPNAEEMVDAVMEMMKE"
     gene            complement(232..954)
                     /locus_tag="VGM24_13025"
     CDS             complement(232..954)
                     /locus_tag="VGM24_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4156354.1"
                     /translation="MGRIFEVRKATMFARWDKMAKQFTRIGKDIAIAVKAGGPDPATN
                     SALRRCFQNAKSVNMPKDRVEAAIKRAMGKEMANYEEILYEGYAPHGIAVLVETATDN
                     PTRTVANVRSYFNKGNGVLGTSGSVSFQFKKLGVFKLRPEGLNMEELELELIDYGLED
                     MGEGEGQHNEPVIILHTAFNDFGNMQRALESRNITPLTAEAEWIPTSPVELSEESAQD
                     VLKLVDKLEQDEDVQKVFHNLA"
     gene            complement(986..2629)
                     /locus_tag="VGM24_13030"
     CDS             complement(986..2629)
                     /locus_tag="VGM24_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY4156355.1"
                     /translation="MSLFVKKPLAGLLAEAADDSGHSLKKTLGAGSLVALGIGAIIGA
                     GLFSITGVAAANQAGPAITISFVVAALGCCFAGLCYAEFASMIPVAGSAYTYSYATMG
                     EFVAWIIGWDLVLEYAVGAATVSISWSRYLIKFLEGFHIHLPDALATGPWDGGMINLP
                     AVFIVVLVSLLLIKGTKESAGVNAVIVIIKVAVVLVFIFLGWKYINTSNYNPYIPKNT
                     GTFGEFGFSGVIRAAAIVFFAYIGFDAVSTAAQEARNPKRDMPIGILGSLAICTILYI
                     LFAHVMTGVTSYTSFRGKDGIAPVAVAIEHMGKADASGILHPDYPWLNRAIIVAILGG
                     YSSVILVMLMGQSRVFFSMSKDGLLPRVFSEVHSKFRTPFKSNFLFMVFVSLFAAFVP
                     ARVVGEMTSIGTLFAFILVCIGVLVMRKNMPDAPRGFKTPFVPVVPILGIATCLFMMV
                     FLPLDTWIRLILWMIIGLDVYLWYGIRHSLLSGGQQNTLSQANKIGGSAGIILSVLLA
                     IVAFAHHRQTGANDLGIYYFSMIFAVAHILVFGRRRSRK"
     gene            2779..>3072
                     /locus_tag="VGM24_13035"
     CDS             2779..>3072
                     /locus_tag="VGM24_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEY4156356.1"
                     /translation="MKKIGLTGWILIAMAAGVAAGLTIHYFAGPAGVASIDRAMSVVT
                     EVFLRLIKMIIAPLVFSTLVTGIAKMGDVRAVGRIGGKTLAWFVCASLLSLILG"
BASE COUNT          731 a          814 c          786 g          741 t
ORIGIN      
        1 ttcattgatg aagatgtgcc ggggggcgcc gcggcctata tgttcaataa ggtgatggaa
       61 gagcagggcg gtttccggtg gctggatgtt tctccgcgaa ccctcacggc aaaagcccac
      121 cggccggcgt atgccagcga cggcgattat ttcagcaagc cgaatgcgga ggagatggtg
      181 gacgcggtta tggaaatgat gaaagaataa tttccagacg ggcaattatt ttcatgccag
      241 gttatgaaac actttctgca cgtcttcatc ctgttctaac ttatcaacca gtttcagcac
      301 atcctgcgcg ctttcctcag aaagttcaac cggagaggtc gggatccatt cggcttcggc
      361 tgtcagggga gttatattcc gggactcgag ggcccgctgc atatttccga aatcattgaa
      421 ggccgtgtgt aatatgataa cgggttcatt gtgttgtcct tctccttcac ccatatcttc
      481 cagcccgtag tcaatcagtt ccagttccag ctcctccata ttgagccctt ccggccttaa
      541 tttgaaaacg cccagtttct taaactgaaa acttacgctt ccgctggtgc ccagcacgcc
      601 atttcccttg ttaaaatagg agcggacatt ggcgaccgtc cgggttgggt tatcggtggc
      661 tgtttccacc agcacagcga tgccgtgcgg cgcatagcct tcatataaga tctcttcata
      721 attcgccatt tccttgccca tggcccgctt tatggcggcc tcgacccggt ccttcggcat
      781 attgaccgac ttggcattct ggaaacatcg cctgagggcc gaattggtgg ccggatccgg
      841 acctccggcc ttgaccgcaa tagctatatc ttttccgatc cgggtgaatt gcttggccat
      901 cttgtcccag cgggcaaaca tggttgcctt tcgtacttca aaaatgcgac ccatagatat
      961 ctgtacttaa ataatagttg aatttttatt ttcttgaccg cctcctgccg aaaaccagga
     1021 tatgcgctac cgcaaagatc atggaaaaat agtaaatgcc cagatcgttg gctcccgttt
     1081 gacggtggtg cgcaaaagcc actatggcca gaagcaccga caggattatt ccggcggaac
     1141 cgcctatttt gtttgcctgt gaaagggtat tttgctgacc tcccgacaac aggctgtgtc
     1201 tgatcccata ccagaggtat acatcaagac caatgatcat ccagaggatc aaccggatcc
     1261 aggtatccag gggcaggaat accatcatga acaggcaggt ggcgataccc agtatgggca
     1321 ctaccggcac gaacggagtc ttaaatcccc gcggggcatc cggcatattt ttccgcatca
     1381 ccagtacccc gatacagacc aggataaagg cgaataaagt gccgatgctc gtcatctcgc
     1441 ccaccacccg ggcaggaaca aatgcagcaa acaggctcac aaacaccatg aacaggaaat
     1501 tgctcttaaa aggtgtcctg aatttggaat gcacttcgga gaagaccctg ggaagcagcc
     1561 cgtccttgct catgctgaag aacacccggc tctggcccat cagcatcacc aggatcacgg
     1621 aagaatatcc gcccaggata gccacgatga tggcgcggtt caaccaggga taatcgggat
     1681 gcaggatgcc gctggcatct gctttaccca tatgttcgat ggcgaccgca accggtgcaa
     1741 tgccgtcttt tcccctgaaa gaagtataac tggtaacacc cgtcatcaca tgggcaaaca
     1801 ggatgtacag gatcgtgcaa atggccaggg agcccagtat cccgatcggc atatcccttt
     1861 ttgggttcct ggcctcctgc gccgcggtac tcaccgcatc aaatccgata taggcaaaaa
     1921 atacaatggc tgcggcgcgt atgaccccgc tgaagccgaa ctccccgaac gttccggtgt
     1981 ttttggggat atatggattg tagttggagg tgtttatata tttccagccc aggaagataa
     2041 aaacaagcac aacggccact ttaatgatca cgataacggc attgacgccg gcgctttctt
     2101 ttgttccctt gatgagcagg aggctcacca gaaccacaat aaaaacagcc ggcagattga
     2161 tcattccgcc gtcccagggc ccggtggcga gtgcatccgg caaatgaata tggaatccct
     2221 ccaggaattt tatcaggtaa cggctccagc tgatacttac cgtggcagcg ccgaccgcat
     2281 attccagcac caggtcccat ccgattatcc aggctacgaa ttcccccatg gtggcgtagg
     2341 aatacgtgta ggcgcttccg gcaaccggaa tcatggaggc aaattctgca tagcaaagtc
     2401 cggcaaagca acagcccagg gcagctacca caaatgaaat ggtgattgcg gggcctgcct
     2461 ggttggccgc cgctacgccg gttatggaaa aaagtccggc cccgatgatg gcgccgatcc
     2521 ccagggcaac cagtgatcct gcgcctaatg tctttttcaa tgagtggccg ctatcgtcag
     2581 ccgcttccgc aagaagaccg gccagcggct tttttacaaa tagactcatg gtgattactt
     2641 attggtgggg tggatgatct ttgagctcgt aaaataagca aatattccgc agacggatat
     2701 tctttttccg tttccgcaaa aacggacatt ttgtgaattg aactatattc gattctttgc
     2761 agccttatta ctgccagtat gaaaaagatc ggattaaccg gatggatcct tattgccatg
     2821 gccgcgggtg ttgctgccgg tttgacgatc cattattttg cgggtcctgc cggggtcgcg
     2881 tccatcgacc gggcgatgtc tgtagtgaca gaggtgtttt taaggctgat taaaatgatc
     2941 atcgcccccc tggttttttc caccctggtg accggcattg cgaaaatggg agatgtgcgg
     3001 gcagtggggc gcattggcgg taaaaccctg gcctggtttg tctgcgcttc tctgctttcc
     3061 ctgatcctgg gt
//
LOCUS       DASYCU010000648         5198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2174022, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000648 DASYCU010000000
VERSION     DASYCU010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174022"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1870
                     /locus_tag="VGM24_13040"
     CDS             <1..1870
                     /locus_tag="VGM24_13040"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEY4156357.1"
                     /translation="GNMGGEIWDARKEIPDWNGPSCDESSWMTAAVYHPRLMLTAQMV
                     EPNRKFYDIRPVSIEKRNDSVFRVDMGVNFAGWTEIKVKGQPGQRVDFLFSERENEEM
                     TFRNHSAYIIGTSGEGIFRNRFNYSSGRWITIKGLKTKPSLTDIRGWVVHTAYESGTS
                     FTCSDSLQNWIYGRTRWNFENLSIGGYVVDCPQRERMGYGGDANATSETGMYNYRLGA
                     FYSKWMEDWRDVQGTKSMEASNYGGNSDDGILPHTAPTYFGGGGPPWGSIVIALPWNF
                     YQHENDKRILEKNFHLIKRWLAFLDSHVKDGLLLRFGGKWDFLGDWLWPNAHAEGMNN
                     DKPENLCFNNCFRIYALRTAAKIARVLGREEEAAQWEGQASASAAVVQTRFFNASDNS
                     YADSSMGNLAIALLAEVPPPALRAAVMHRLEKEILVVRHGHIHAGITGGAVLFKLLRE
                     AGRDDLLFSMTSQTDYPGWGYMKANGATSLWEMWEKDLPGHSLLHSSYLYPGAWYIEG
                     VGGIRPDSAHPGFRRFKVRPPLLKPSQLAWAKTSFESPAGVISTYWIRRAGRLTLDVT
                     VPPNCTATVYFPSGHPDEIKISSPQAKNAGKEDHYSLYSLPAGKYELSGKEDPVTE"
     gene            complement(1882..2343)
                     /locus_tag="VGM24_13045"
     CDS             complement(1882..2343)
                     /locus_tag="VGM24_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY4156358.1"
                     /translation="MVTTKIKSGISVKSAAFSQGGQIPKKYSCEGENINPPLEISGFP
                     PDTRSLTIILEDPDAIKGIFYHWIAWNIPPDEFIPENSAPGTTGRNSGGEQKYMGPCP
                     PTGSHRYHFRVFALDAPLDLPAGSDIKGLEEAMHGHQLSTGEIMAHYRKGG"
     gene            2725..4698
                     /locus_tag="VGM24_13050"
     CDS             2725..4698
                     /locus_tag="VGM24_13050"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEY4156359.1"
                     /translation="MNRITTVFLLYSLLSTTPLSAQKKISLNSPDKKIGFAFTLVAGA
                     PEYSVLFNKQPLIGHSRLSLSFENNEGWGSDLTIDHATFTDGEDNYTLPEGKTSRVHD
                     FYKEVSIPLRERSGKKRLIILRVRAFNDGLAFRYEFPAQDNWKAYALTDENTQVNLTG
                     DPTARVAFLENFLTSHEHRYNILPLKDIKNDTLMDMPALFEFPGKIWLAVTEANLVNY
                     AGMSLIKRNGILTSQLSPLPGQTEIKVKAVLPHQTPWRVLMISNRIGALIESNILTSL
                     SEPCKIKDLSWLKPGKATFHWWNGDTLPDTTFEAGINFDFNKYYIDFASRNGIEYHTI
                     IGNRGVAWYQNDGMEYQPGPNTDVTKPRPTLDIQAICDYAKSKGVGIRFWIHWQALYP
                     KLDTAFALFQKWGVKGMMVDFMDRDDQEMVRIQEEILQKAAMHHLEIQFHGAFKPTGL
                     SRTYPNESTREGTLNYENDKWGNLITPDDDIEIPFTRGLAGPTDYHLGGFRAMPASRF
                     KVQVSRPHVLATRCHMLAMYVVLENHLSMVCDEPEAYEGQDGFDFLRKVPTVWDQTVV
                     PGAETGEWVTLARRKGTDWWLGTINNSKPRTVSIPLSFLPAGNYHADIYSDAPDVAEN
                     PNHLVKETREVSSSGSLTINLPAGGGQVVILRP"
     gene            4788..5165
                     /locus_tag="VGM24_13055"
     CDS             4788..5165
                     /locus_tag="VGM24_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY4156360.1"
                     /translation="MKSEKAGGVDQYIAGFPKETQKLLQEIRMTIRQVAPDAEEGISY
                     QMPAYKDHGILVYFAGYKNHIGFYPTASGIAAFRKELSAYKGAKGSVQFPLDKPLPLA
                     LISKILAFRLKENLKKAEIKTKK"
BASE COUNT         1301 a         1335 c         1374 g         1188 t
ORIGIN      
        1 cggaaatatg ggcggagaga tctgggacgc caggaaagaa ataccggact ggaacggacc
       61 gtcctgcgac gaaagctcgt ggatgactgc ggctgtctat catccgcggc tgatgctgac
      121 ggcccagatg gtcgaaccca accggaagtt ctatgacatc cgcccggttt ccattgaaaa
      181 gcgaaacgac agtgtatttc gggtagacat gggcgttaat tttgccggat ggacggaaat
      241 taaagtgaaa ggacagcccg gccaacgggt cgacttttta ttctccgagc gggaaaatga
      301 agagatgact ttccggaacc acagcgccta tattatcgga acatccgggg aaggcatttt
      361 ccgcaaccgc tttaattaca gttccggaag atggataacg ataaaaggat tgaaaacaaa
      421 accgtcactc accgatatcc ggggctgggt tgtccatacc gcttatgaat cgggaacttc
      481 gtttacgtgt tcggattccc tgcagaactg gatttacggg cgcacccgct ggaattttga
      541 aaatctttcc atcggcggat acgtagtcga ttgcccgcaa cgcgaacgga tgggctatgg
      601 cggcgatgcc aatgccacat cggaaaccgg aatgtacaat taccggctgg gtgcgttcta
      661 ttcaaaatgg atggaagact ggcgggatgt gcagggaaca aaatcgatgg aggcaagcaa
      721 ttacggcggc aacagcgacg acgggatatt accgcacacg gcaccaacct atttcggggg
      781 cggcggcccg ccctggggta gcatcgtcat cgctcttccc tggaactttt atcagcatga
      841 aaacgataag cggatcctgg aaaagaattt tcacctgatc aaaagatggc tcgctttcct
      901 ggattcccat gtaaaagacg gactgctgct tcgtttcgga gggaaatggg atttcctggg
      961 cgactggcta tggcctaacg cccatgccga gggaatgaat aatgacaagc cggaaaatct
     1021 ttgttttaat aactgcttcc gcatatatgc gctgcgcacg gctgccaaga ttgcgcgggt
     1081 attaggcagg gaggaagaag cggcccagtg ggaaggacag gcatccgcct ccgctgctgt
     1141 ggtgcagacg cggttcttca atgcatccga taacagttat gccgacagtt ccatgggcaa
     1201 cctggccatc gccctgctgg cggaagtgcc gccgccggcg cttcgcgcgg cggtgatgca
     1261 tcgcctggaa aaagagatcc tggtggtccg gcatggccat atccatgcgg gcattacggg
     1321 cggcgcggtc ctgttcaagt tattgcgcga agctggccgg gacgacctgc ttttcagcat
     1381 gacctcgcaa acggattatc ccggatgggg ctatatgaaa gcaaacggag caaccagtct
     1441 ctgggaaatg tgggagaagg acctgcccgg tcattccctc ctgcatagct cgtatttgta
     1501 tccgggggcc tggtatatcg agggtgttgg cggaatccgg ccggatagcg cccatcccgg
     1561 cttccgccgt ttcaaagtcc gacctcccct gcttaaacca tcgcaactcg cctgggccaa
     1621 aacttccttc gaatcgccgg caggcgtgat cagcacctac tggatccgta gggccggcag
     1681 gctgaccctg gatgtaaccg ttccacccaa ttgcacggct acggtctatt tcccctcggg
     1741 ccatccggat gaaatcaaaa tatcctcacc acaggccaag aatgcgggca aagaggatca
     1801 ttacagcttg tattcgcttc ctgccggtaa atatgaactg tccggaaaag aagatccggt
     1861 gacggaatag ggctttattt ttcagcctcc tttccggtaa tgggccatta tttccccggt
     1921 ggaaagctgg tggccatgca tggcttcttc caaccctttt atatccgaac cggccggaag
     1981 atccagcggg gcatccagcg caaacacccg gaaatgatac cggtgggatc cggtcggcgg
     2041 acagggcccc atatattttt gctcaccccc gctgtttcgg ccggtagtcc cgggagcgct
     2101 gttttccgga atgaattcgt cgggcggaat attccaggcg atccagtgat agaaaatccc
     2161 tttgatcgcg tccggatctt ccaggataat ggttagtgac cgggtatccg gggggaaccc
     2221 actgatttcg agcggtgggt tgatgttctc cccttcgcag gaatattttt tcggaatttg
     2281 tccgccctgg ctgaaggccg cactcttaac agatatgccg gattttattt ttgtcgttac
     2341 cataacttaa agattgacgg tttctaattt tctccttata aaccgagctt caaaaaacag
     2401 accaacccgg cctgcctggg aaattcccgc aagactattt tcatcctcct ccatgaggtc
     2461 ctttcctacc atcccaaaac ccactggcag ccctaccgga catcccgcct tcttcccggt
     2521 tttacgcaaa gaaaatcatc agcaggccgg atgattttca aagaaattat tacaccacac
     2581 tcactcttat taacctgttg tccatcctgg tcgtattgtg gatgaccttc gtaatcgtgc
     2641 atgaccgcgt tcttcatgac tgttgctgaa accgggaacg gattggggtt tatttcgttt
     2701 atttgttgct aattcgccgt aataatgaac cggataacga ctgtctttct tctttattca
     2761 ttgctcagca caaccccgct ttcggcacaa aaaaagatca gcctcaactc tcccgataaa
     2821 aaaatcggct ttgcttttac cctggtggcc ggtgctcctg aatattctgt actgttcaat
     2881 aaacagccac tgatcgggca cagccggctc agtctctcct ttgagaacaa cgagggctgg
     2941 ggttcggatc ttacgattga tcacgctacg ttcaccgacg gagaagataa ttataccctg
     3001 ccggagggaa agaccagccg ggtgcacgat ttttataaag aagtgagcat tccgctgcgc
     3061 gagcgcagcg ggaaaaaaag actgatcatt ttacgggtcc gggcgtttaa tgacggcctt
     3121 gccttccggt atgagttccc tgcccaggat aactggaagg cctatgcgct taccgatgaa
     3181 aatacgcagg ttaacctgac cggcgatccg accgcccggg tcgccttcct ggaaaatttt
     3241 cttacttccc acgagcaccg ctacaatatc cttcccctga aagatattaa aaacgatacc
     3301 ctgatggaca tgcccgccct ctttgagttc ccgggaaaaa tatggctggc ggtaacggaa
     3361 gccaacctgg tcaattatgc cggcatgtcc ctgatcaaac gaaacggcat actcaccagc
     3421 cagctcagtc cgctgcccgg acaaaccgaa atcaaagtga aagccgtatt gccgcaccaa
     3481 acgccgtggc gggtattgat gatcagcaat cgcatcggcg cgctgatcga gtccaacata
     3541 ctgaccagcc tgagtgagcc ctgcaaaata aaagatctct cctggctcaa gccggggaag
     3601 gccacttttc actggtggaa cggggatacg ctgcccgata ccacgttcga agccgggatt
     3661 aattttgatt tcaataaata ttatatcgat ttcgcttcgc ggaacgggat tgaataccac
     3721 acgatcatcg ggaaccgcgg cgtggcctgg tatcagaatg acggcatgga ataccagccc
     3781 ggcccgaata cggatgtcac caaacccagg ccgaccctgg atatccaggc gatctgcgat
     3841 tacgccaaat ccaaaggcgt gggtatccgc ttctggattc actggcaggc cctgtatccc
     3901 aaactggata ctgcattcgc tttatttcag aaatggggtg tgaaagggat gatggttgat
     3961 tttatggacc gggatgacca ggagatggtc aggatacagg aagagatcct gcagaaagct
     4021 gctatgcacc atctcgagat ccagtttcac ggggcattca aaccgaccgg tctgagtcgc
     4081 acttatccca atgaatccac ccgggaggga acgctcaact atgaaaatga taaatgggga
     4141 aacctgatca cgccggacga cgatattgaa attccattca cgaggggact cgcaggaccg
     4201 accgattatc atttgggggg cttccgcgcc atgccggcct cgcggttcaa ggtgcaggtc
     4261 agccgtcctc atgtactcgc aacgcgctgt catatgctgg cgatgtatgt ggtcctggaa
     4321 aatcatttgt ccatggtatg cgacgaaccg gaagcgtatg aaggccagga tggttttgat
     4381 tttttgagaa aagtgccgac agtatgggat caaacggttg ttcccggagc agaaacgggt
     4441 gagtgggtga cccttgcccg gcggaaagga acggactggt ggctgggaac cattaacaac
     4501 agtaaaccca ggaccgtaag cataccgctg agttttctgc cggcgggtaa ttatcatgcc
     4561 gacatttatt ccgatgcccc ggacgtggca gagaatccga atcacctggt aaaagaaacg
     4621 cgggaggttt cctcgtccgg cagcctgacg attaacctgc cggcgggagg cgggcaggtt
     4681 gttattctgc ggccctgatt tgacgcgcta taggaaatcg tttttcagca gcaagcaatc
     4741 ttcggaaggc tggcattttg ttaatgcttt aaataagaca actcatcatg aaatctgaaa
     4801 aagcgggcgg agtagaccag tatattgccg gttttccaaa agaaacacaa aaactgttgc
     4861 aggagatccg gatgaccatc cggcaagtgg cgccggatgc agaggaaggg atcagctacc
     4921 aaatgccggc ctataaagat catggaatac tggtttattt tgcgggatat aaaaaccata
     4981 tcggatttta tccgaccgct tccgggatcg ccgcgttccg gaaagaactc tctgcttata
     5041 agggagcaaa aggttctgtt cagttcccgc tggacaagcc ccttccgctg gccctgatca
     5101 gcaaaatact ggccttccgg ttaaaggaaa atttgaaaaa agccgaaata aaaacaaaga
     5161 aataaattca attctttcag atccgccggg caatgaag
//
LOCUS       DASYCU010000649         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4492535, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000649 DASYCU010000000
VERSION     DASYCU010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4492535"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGM24_13060"
     CDS             <1..211
                     /locus_tag="VGM24_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156361.1"
                     /translation="KGSDFQLNRFYKEFAVGAGTGLRIDFNYFLIRLDWAYKIRDPQR
                     IDNPDKSVYKFLAGGQLQLGINYPF"
     gene            complement(188..865)
                     /gene="truB"
                     /locus_tag="VGM24_13065"
     CDS             complement(188..865)
                     /gene="truB"
                     /locus_tag="VGM24_13065"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY4156362.1"
                     /translation="MENPFEAGRMILINKPQHWTSADVVKKIRNLVNAKVGHAGTLDP
                     LATGLLILCTGKFTKKISEYMSQEKEYTGTFTLGATTASDDLENDPGNFKDYSGITDF
                     QLQAAALSLTGEILQMPPAFSAVKKNGRRAYDLARQGKTVVLDPRKITIRHFELRSSA
                     LPVIDFRVICSTGTYIRSLARDLGEALGCGAYLSKLCRTRIGSYTLEDSLTVEEAEKK
                     LKGIIDA"
     gene            complement(887..2233)
                     /locus_tag="VGM24_13070"
     CDS             complement(887..2233)
                     /locus_tag="VGM24_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY4156363.1"
                     /translation="MNLLKRFYLSLFFGKWFFRLLASLIFLFVLSYGLPFLFEITQLL
                     LLLFLMACLLDWSLLFLNRKGITVQRNLPERLSNGDENHIQLSIKNDYPFRILLQLID
                     ELPEQLQIRDFLIEDFILPQDKKILPYTVRPKERGEYFFGNIQAFVKSPLRLIVRRKT
                     FYAKQAVKVYPSFLLLRQFEFMAHITDPGNIGFKQVRKTGHSLEFEQIREYVNGDDIR
                     SINWKATGRTGGQLMINSYADEKSQQVYCIIDKGRVMKMPFGGLTLLDYAVHATLIMS
                     GVALSRQDKSGLITFAEKGGDFIPANHRAIQMNSILNALYKLETRFLESDYAFLHALV
                     KTRIPQRSLLILFTNFESLNSLGRQLPYLRNISKKHLLLVVFFENTPLQALASGNAEH
                     IDRLYEKVIAEKFVLEKKLIVKELQRYGIHALLTAPEKLTVDVVNKYLQIKARREI"
     gene            complement(2236..>2871)
                     /locus_tag="VGM24_13075"
     CDS             complement(2236..>2871)
                     /locus_tag="VGM24_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY4156364.1"
                     /translation="LIDEINRAPAKTQAALFEVMEERQVTVDGQTMKMQQPFLVVATQ
                     NPVEQEGTYHLPEAQLDRFLFKIKVDYPNAEEEYEVVMKHHSSNGSSLGDNVEPVLNA
                     SIIASLRKQVSTIHVDEKLIRFIVSVVASTREHKSIYLGASPRASIGILQASKAIAAM
                     NGRDFVVPEDILYVIPAVLRHRLVLTPEKEMEGDTPDEVIREVIQSLEIPR"
BASE COUNT          714 a          732 c          668 g          757 t
ORIGIN      
        1 aaagggaagt gattttcagc ttaaccggtt ctataaagaa tttgccgttg gcgccggcac
       61 cggcttacgc atagatttta attattttct gatccgcctg gactgggcct acaagatccg
      121 ggatccgcag cgcattgaca atccggataa atccgtttac aaattcctgg ctggcggcca
      181 gttgcagtta ggcatcaatt atccctttta attttttttc agcttcctcc accgtaaggg
      241 aatcttccag ggtataagaa ccgatcctcg tccggcatag tttgctcagg taagccccgc
      301 agcccagggc ttcccccagg tcccgggcca aactgcggat gtacgtgccc gtggaacaga
      361 tcacccgaaa atcaataacc ggtaaggcgg aagaccgcaa ttcgaaatgg cggatcgtga
      421 ttttcctggg atccagaacg actgttttac cctggcgcgc caggtcataa gcccggcggc
      481 catttttttt aacggctgaa aaggccggag gcatctgcag gatttccccg gtgagggaca
      541 aagcggcggc ctgtaactga aaatccgtta tccccgagta atccttaaaa tttccgggat
      601 cgttttccag gtcatcagat gccgtggtcg cacccagggt aaaggttccc gtatattcct
      661 tctcctggct catatactca ctgatctttt ttgtaaactt tccggtgcac aggatgagca
      721 aacccgtagc gagcggatcg agggtaccgg catgacccac ttttgcattc accaggttcc
      781 tgatcttctt caccacatcg gcggaggtcc agtgctgcgg cttgttgatc agtatcatcc
      841 ggccggcttc aaacggattt tccatattct caaatgtatg tagaaattaa atttcgcggc
      901 gtgctttgat ctgcaggtat ttgtttacga catccaccgt taatttttcg ggtgccgtta
      961 ataaggcatg aatgccgtac cgctgcagtt cttttacgat caattttttt tccaggacga
     1021 acttttcagc aattaccttt tcgtacaacc ggtcaatatg ttccgcgttc ccggaagcca
     1081 gggcctgaag cggcgtattt tcaaaaaaaa ccaccagcag caaatgtttt tttgagatat
     1141 tccggagata aggcaattgc ctgccaaggg aattgaggga ttcaaaattg gtgaacagga
     1201 tcagtaagct tctctggggt atccttgttt ttaccagcgc atgcaaaaaa gcataatccg
     1261 attccaggaa acgggtctcc agtttataca aggcattcag aatactgttc atctggatag
     1321 cgcggtggtt ggccggaata aaatcgcctc ccttctctgc aaaagtgatc aggcccgatt
     1381 tatcctgccg ggataaggcc actccgctca tgatcagcgt ggcatgcacg gcataatcca
     1441 gcagggtcag cccgccgaaa ggcattttca ttacccgccc cttatcgatg atgcaataga
     1501 cctgctggct tttttcatcc gcataactat tgatcatgag ctgtccgccc gtccttccgg
     1561 ttgctttcca gttgatgctc cggatgtcat ctccgttaac gtattcccgg atctgttcga
     1621 attccagact atggccggtt ttccgaacct gtttaaaccc gatattaccc ggatcggtga
     1681 tgtgtgccat aaactcgaac tgccgcagca gcaaaaaact gggatagact ttcactgcct
     1741 gttttgcgta aaacgttttc cgccgcacga tcaggcgtaa agggcttttc acaaaggcct
     1801 ggatattccc gaagaaatat tctccccttt cctttggccg gacggtataa ggcagtattt
     1861 ttttatcctg cggaagaata aaatcctcta tcagaaaatc ccggatctgc aattgttccg
     1921 ggagctcgtc aatcaactgg agcaatatcc ggaaagggta atcatttttt atgctcagct
     1981 gaatatgatt ctcatctccg ttgctcaatc gctccggcag atttctctga accgttatgc
     2041 ccttccggtt taaaaataaa agagaccagt ccagaaggca ggccatcaga aacagaagaa
     2101 gcagcagctg cgttatttca aacaggaaag gtaggccata ggaaaggaca aataaaaaga
     2161 taagcgaagc cagcaaacgg aaaaaccatt tgccaaaaaa aagagaaaga taaaatcgtt
     2221 taagaaggtt catttttatc tcggaatttc caatgactga atcacttccc ggatcacctc
     2281 atcgggggta tctccctcca tttccttttc cggcgtcaat accaggcgat gccgcaaaac
     2341 agccggaatc acgtacagga tatcttcggg aacaacaaag tcccttccat tcattgccgc
     2401 aatggcctta gaggcctgca gaatgccaat ggaagcgcgg ggggaggcgc ccaggtaaat
     2461 ggatttatgt tcccgcgtgg aagccaccac ggatacaata aaacggatca gtttctcgtc
     2521 cacgtggatc gtgctcacct gtttgcggag ggaagcaatt atggaagcat tcagcaccgg
     2581 ttccacgttg tccccgaggg aactgccgtt actgctatgg tgtttcataa ccacttcgta
     2641 ttcttcttct gcattggggt agtccacttt tattttgaac aggaaccggt cgagctgcgc
     2701 ttccggcaaa tgataagttc cttcctgctc caccggattc tgggtagcca ccaccaggaa
     2761 aggctgctgc attttcatcg tctgcccgtc tacggtaacc tgccgttctt ccatcacttc
     2821 aaacagcgcg gcctgcgttt ttgccggggc cctgttaatt tcatcaatga g
//
LOCUS       DASYCU010000650         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4348045, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000650 DASYCU010000000
VERSION     DASYCU010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4348045"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VGM24_13080"
     CDS             <1..671
                     /locus_tag="VGM24_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156365.1"
                     /translation="LTMKFFLPAGLLMVFSFIGPSRTTAQSAEPWKPYRVQPSHPYFL
                     QSRKLLNSKKKIELGNNIADFYLYRNGELVKPPKNGHYDFFPAGCLCFKYDDTLLLNS
                     GVGASVGVGVGIKIYSGMFTSSLHANNKNRQIYKLSEEDSVYQESILAEPETQSLKLL
                     RQPDFVPNEVIIGEYRATYKKFYRKNARSRDEACRYTVRIVFKCRVTGGIDSMKSLTG
                     MNTQ"
     gene            709..2091
                     /locus_tag="VGM24_13085"
     CDS             709..2091
                     /locus_tag="VGM24_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEY4156366.1"
                     /translation="MATTDQSASFLDKSQTKAIDLEHRKKINFNIGKYEAIVPQGKQQ
                     FENVTLARERAKNIKWRALETLDQQLERFEQNFSSRGGKVIWAENAEEALNEIISICR
                     EKQCKTIVKSKSMVTEEIHLNDFLEKHGIESVETDLGEYIQQLDHEPPYHIVTPAMHK
                     SKEDIAQLFHEKLHSVPGLSPAELTLVAREKLRAKYLEATIGITGANFIIAETGSIAI
                     SENEGNGRLSCSFPQTHIVITGIEKLLPSLTDLALFWPLLSTFGTGQRVTTYNSIVSG
                     PKQPGENDGPDEMYVILLDNGRTNLLSNVKVRESLYCIRCGACLNACPVYKNIGGHTY
                     GTTYSGPIGAVITPHLKGIKQWNHLSYASSLCGNCTEVCPVKINLHEILLENRYEAVQ
                     EGDASWMEKIAWRGWKQASLHRKWMNMGNHKMKNWMINRMFKGWTRQRADLRFPEKTF
                     NEYWKERKNR"
     gene            2146..>2732
                     /locus_tag="VGM24_13090"
     CDS             2146..>2732
                     /locus_tag="VGM24_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156367.1"
                     /translation="MIGREIFYEPISLTTDKTRFLEESGPKINYSSSRLTADEFFIRN
                     HPLLFETGIREQKVDCFSASGQVAFFETGGDFPFDIFAASFYLLSRYEEWLPFKPDKY
                     GRFPYHTSLAFRENFLDIPLVNYWLEHFKKRLQEKFPELLFRIKDFKIIPSYDIDIAY
                     SYKYKGLFRNAGGFARSLLKGEWARLLDRWDVLFQ"
BASE COUNT          776 a          647 c          651 g          658 t
ORIGIN      
        1 ccctgactat gaaatttttc ttaccggctg gtttattgat ggttttttct ttcatcggtc
       61 cttcccgcac tacggcccag tccgcggagc cctggaaacc ataccgtgtt cagccttccc
      121 acccgtattt cctccagtcg agaaaactcc ttaacagtaa aaagaagatt gaactcggaa
      181 ataatattgc agatttttat ctgtaccgga atggggaact ggtcaaaccc ccaaaaaatg
      241 ggcactacga ttttttcccg gcgggatgcc tttgttttaa atacgacgat actttattgc
      301 tgaacagcgg ggtgggtgct tccgtgggcg tgggggtcgg aattaaaatt tattccggta
      361 tgttcaccag ctccctgcac gcaaataata agaacaggca gatttataaa ttgtccgaag
      421 aggacagcgt ttatcaggaa agtatcctgg ccgaaccgga aacccagtca ctcaaattac
      481 tccggcaacc tgattttgtg cccaatgaag tgattatcgg ggaataccgg gcaacctata
      541 agaaattcta cagaaagaat gcccggtccc gggatgaagc ctgccggtat acggtcagga
      601 tcgtattcaa atgccgggta accgggggaa ttgacagcat gaagagcctt accggcatga
      661 acacgcagta gccgccagcc cctccaacat atttattaac ttcatcccat ggccacaaca
      721 gatcagtcag ctagtttttt agataaaagc cagaccaaag caattgacct ggaacaccgc
      781 aagaagatca attttaatat tggtaagtat gaagccattg ttcctcaggg aaagcagcag
      841 tttgaaaatg taaccctcgc aagggaacgg gcaaaaaata ttaaatggcg tgcgctggag
      901 accctggacc agcaactcga acgatttgaa cagaacttta gcagcagggg cggaaaagtg
      961 atctgggcgg aaaatgcgga agaagcgctg aatgaaatta tctctatctg ccgcgaaaaa
     1021 caatgcaaga ccatcgtcaa gagcaaaagt atggtcaccg aagaaattca cctcaatgat
     1081 tttctggaga agcacggcat tgaaagtgtt gaaacggacc tgggagaata tatccagcag
     1141 ttggaccatg agccccctta ccatattgta acccctgcga tgcacaaaag caaggaagac
     1201 atcgcacagc tttttcatga aaaacttcat tcggtccccg ggctttcgcc ggctgaactc
     1261 accctggtag cgcgggaaaa actgagggca aaatatctgg aagcgaccat cggcatcacc
     1321 ggggcgaatt ttatcattgc cgaaacggga agcattgcca tttccgagaa cgaaggcaac
     1381 ggaaggctca gctgttcatt cccccaaacc catattgtaa ttacaggaat tgaaaaactg
     1441 cttccttccc tgaccgatct cgccctgttc tggcccctgc tatccacttt tggtaccgga
     1501 cagcgggtaa ccacgtacaa ttccatcgtt tccggaccga aacagcccgg agaaaatgac
     1561 ggtccggacg agatgtacgt cattctgctg gacaatggaa gaaccaactt actttccaat
     1621 gtaaaggtgc gggaaagcct gtattgtatc cgttgcggag cctgcctgaa tgcctgcccg
     1681 gtttataaaa atatcggcgg ccatacttac ggtactacct atagcggacc gatcggtgcc
     1741 gtgatcacgc cgcatcttaa gggaataaag cagtggaatc acctcagtta cgcatcctcc
     1801 ctctgcggga attgcacgga agtttgtccc gtgaaaatca acctgcatga aattctgctg
     1861 gaaaacaggt atgaagccgt tcaggaaggg gatgcctcct ggatggaaaa gatcgcctgg
     1921 cgcggatgga aacaggccag cctgcaccgg aaatggatga atatgggtaa tcataaaatg
     1981 aaaaactgga tgatcaaccg gatgtttaag ggatggacca ggcaaagagc agatctccgg
     2041 tttcctgaaa agacgttcaa tgaatattgg aaagaaagaa agaacaggtg acccatgctg
     2101 ctccatagcc actatacctc tccgcggcta tcttatataa tcgggatgat cggaagggaa
     2161 attttctacg aaccgatcag cctgactacc gataaaacaa gatttcttga agaatccgga
     2221 ccaaagatta actactcttc ctcccgctta actgcggatg agttttttat tcggaaccat
     2281 ccgctcctct ttgaaaccgg tatccgggaa caaaaggtcg attgtttttc cgcctccggt
     2341 caggtggcgt tttttgaaac ggggggtgat tttccgttcg atatttttgc ggccagtttt
     2401 tacctcctga gccgctatga agaatggctc ccgtttaaac cggataaata cggaaggttt
     2461 ccctatcata catcactggc tttccgggag aatttcctgg atatccccct ggtcaattac
     2521 tggcttgaac actttaaaaa acgactgcag gagaaatttc cggaactgct tttccggata
     2581 aaggatttca agattatacc cagttacgat atagacatcg cttattcata taaatacaag
     2641 gggcttttcc ggaatgccgg aggttttgca cgatcccttc tgaaaggcga atgggcccgg
     2701 ttgcttgacc gctgggatgt tttgtttcag aa
//
LOCUS       DASYCU010000651         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3332236, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000651 DASYCU010000000
VERSION     DASYCU010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3332236"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGM24_13095"
     CDS             complement(<1..294)
                     /locus_tag="VGM24_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY4156368.1"
                     /translation="MGAIAFSAEKLAKVKELISHYPEGKQKSAILPVLHLAQDNAGGW
                     LSTETMDYVAELLHILPVEVYEVATFYSMYNMKPVGKYVFEVCQTGPCMINGSD"
     gene            complement(331..1557)
                     /locus_tag="VGM24_13100"
     CDS             complement(331..1557)
                     /locus_tag="VGM24_13100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY4156369.1"
                     /translation="MPDQQHIKLTEGSIEKTTTTLNLGPTHPATHGVFQNILELDGER
                     IVSAEQTVGYIHRAFEKIAERRPLYQITPLTDRLNYCSSPINNMGWHLTCEKLLNVKT
                     PKRVDYLRVVIMELARISDHLVCNSVVGVDSGAFTGFLYVMQYRELIYEIYEEVCGSR
                     LTTNMGRIGGFERNFSNTAFEKLERFLKEYPGVLKEFENLFNRNRIFMDRTIGTGGIS
                     GERALNYGFTGPNLRATGVDYDVRVHTPYSSYEDFDFNIPVGKTGDNYDRFLVRNQEM
                     WESLKIIEQAYRKLQEFKGADAEVYHADAPEYFLPPKQEVYTKMEALIWHFKIVMGET
                     EIPPGEVYQSVEGGNGELGFYFISDGGRAPYRLHFRRPCFIYYQAYSELTKGSMLSDA
                     VLTMSSLNLIAGEMDA"
     gene            complement(1666..2172)
                     /locus_tag="VGM24_13105"
     CDS             complement(1666..2172)
                     /locus_tag="VGM24_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY4156370.1"
                     /translation="MALTNETIKERLEKHFEGRLSRFEEPYGMLSFEAPKELNLKVMQ
                     FLYDDEELRFQFLTDLTAVHYPHQPGRELAVLYHLHNLRDNVRIRFRIYTDIKTPDVF
                     TATQLYAASNWMERETYDFFGVNFVGHPNLKRILNVDEMDYFPMRKEYPLEDQTRTDK
                     DDEMFGRG"
     gene            complement(2196..2723)
                     /locus_tag="VGM24_13110"
     CDS             complement(2196..2723)
                     /locus_tag="VGM24_13110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEY4156371.1"
                     /translation="MVDMPEGYTGEGFFATKLSDVVGLARKNSLWPLPFATSCCGIEF
                     MATMASHYDLARFGAERLGFSPRQCDLLMVIGTIAKKMGPIVKQVYLQMAEPRWVMAV
                     GACASSGGIFDTYSVLQGIDQIIPVDVYVPGCPPRPEGILDGFMRIQDLVGGESVRRR
                     SGEKYRELMQSYGIK"
     gene            complement(2881..2982)
                     /gene="ndhC"
                     /locus_tag="VGM24_13115"
     CDS             complement(2881..2982)
                     /gene="ndhC"
                     /locus_tag="VGM24_13115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY4156372.1"
                     /translation="MGWSGFAEVLLFVAFFLIGFVYIIKKGALQWED"
BASE COUNT          686 a          826 c          675 g          796 t
ORIGIN      
        1 atcgctgccg ttgatcatgc agggcccggt ctggcacact tcaaatacat atttccccac
       61 cggcttcatg ttgtacatgg aataaaaggt ggccacttca tacacttcca ccggcagaat
      121 atgcagcagt tccgccacat agtccatggt ttccgtgctc agccagccgc cggcattgtc
      181 ctgggccaga tgaagtaccg gaagaatggc actcttctgc tttccttcag gataatgact
      241 gatcagttct tttactttgg ccagcttctc ggctgaaaag gcaatagcac ccacggcttg
      301 atatttttaa taaaagacta aggttaacga ctacgcatcc atttcgcccg cgatcaggtt
      361 cagactgctc atggtcagca cggcgtcact cagcatggag cctttggtca gttcggaata
      421 agcctggtaa taaataaaac agggcctgcg gaaatgcagc cggtaaggcg cccgtccgcc
      481 atcgctgata aaataaaatc caagttcccc gtttccgcct tccacactct ggtaaacttc
      541 tcccggaggt atttccgttt cccccatcac gatcttgaag tgccagatca gcgcttccat
      601 cttggtatat acttcctgtt tgggcggaag aaaatattcc ggggcatccg catgatacac
      661 ttcggcatcc gcccccttga attcctgcag tttccggtaa gcctgttcga taattttcag
      721 cgactcccac atttcctggt tacggacgag gaaccggtcg taattatctc ctgttttgcc
      781 taccgggata ttaaaatcaa aatcctcata gctggaatag ggagtgtgca cgcgaacatc
      841 gtaatcaacc cccgtggcgc gcaggttagg tccggtaaat ccgtaattga gcgcgcgttc
      901 tccgctgatg ccgccggtgc cgatcgtacg gtccatgaag atgcggttcc ggttgaacag
      961 gttttcaaat tccttcagca cgccgggata ttctttcaga aaacgctcga gtttttcaaa
     1021 ggcggtattg ctgaaatttc tttcaaaccc gccgatccgt cccatattgg tggtgagccg
     1081 cgatccgcaa acctcttcat agatttcata aatgagctcc cggtactgca ttacgtagag
     1141 gaacccggtg aatgcgcccg aatccacgcc caccacggaa ttgcagacca ggtggtcgga
     1201 gatcctcgcc agctccatga tcaccacccg caggtaatcc acccgcttgg gcgtttttac
     1261 gttcaataat ttttcgcagg tcaggtgcca gcccatatta ttaatcggag aagaacaata
     1321 attcaggcgg tcggtgagcg gggtgatctg atacagcggc cttctttcag cgatcttttc
     1381 aaaggcccgg tgaatatagc cgacggtttg ttctgccgat acgatccgtt cgccgtccag
     1441 ctccaggata ttttggaata ccccgtgggt cgccggatgc gtgggaccca ggttcagggt
     1501 cgttgtggtc ttctcaatac tcccctcagt taattttata tgttgctgat ccggcatggt
     1561 aaagctggtt tgatgcaata ttttttgcag tgctcctttt tcaatttgcg ttcatcgttg
     1621 ttcccgggtt ccgttttcat atccccaccc cggcgcattt tcagatcatc ctctcccgaa
     1681 catctcgtcg tccttatccg tccgggtctg atcctccagc gggtattcct tccgcatggg
     1741 gaagtaatcc atttcatcta cattcaatat ccgtttgagg ttcgggtggc cgacaaaatt
     1801 aactccgaaa aaatcatagg tttccctttc catccagttc gacgcagcgt ataattgggt
     1861 ggccgtaaac acgtccgggg ttttaatatc cgtatagatt ctgaaccgga tccggacatt
     1921 atcccgcaaa ttgtgcaggt gatacagcac ggccagctcc cggcccggct gatgggggta
     1981 atgaaccgcc gtcaggtcgg tcaaaaattg aaaacgcagt tcctcatcat cgtagagaaa
     2041 ctgcattact ttcagattca gctctttcgg agcttcgaaa ctgagcattc cgtaaggttc
     2101 ttcaaaacgg gacaaccgtc cttcaaaatg tttttccagt ctttccttta ttgtttcgtt
     2161 cgtcaacgcc atggcgttaa tttgataatt tcaaattact tgatcccgta actctgcatc
     2221 agttcccggt atttctcccc gctccttctc ctcacggatt ccccgccgac caggtcctgg
     2281 atccgcataa agccgtcgag gattccttcc gggcggggcg gacatccggg cacgtacacg
     2341 tctaccggta tgatctgatc gatcccctgc aatacggaat aggtgtcgaa tattcccccc
     2401 gaagaggcgc aggcaccaac ggccattacc cagcggggtt cagccatctg gagatacacc
     2461 tgttttacaa tcgggcccat cttttttgcg atcgtgccga tcaccatcag caggtcgcac
     2521 tgccggggag aaaatcccaa cctttccgca ccaaaacgcg ccaggtcgta gtgggaggcc
     2581 atggtggcca taaactcgat accgcagcag gaagtggcaa aaggcagggg ccacaaagaa
     2641 ttctttctgg ccagtcccac cacgtcactc agcttcgtag caaaaaaacc ttcaccggta
     2701 tatccttccg gcatatccac catgctgata tggtctttgg aatcggccgg gttggtcttt
     2761 acattaaatc tgactggtct tgccattttt attcatttta catccccgga ggatgattca
     2821 ttctaaaact gcattccatt ctctcctatg gcattccgca tcctccattt tttattcgga
     2881 tcagtcttcc cattgcaatg cccctttttt aatgatatac acaaatccga tcaggaaaaa
     2941 ggcgacaaat aaaagcactt cggcaaatcc gctccatccc agt
//
LOCUS       DASYCU010000652         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1592951, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000652 DASYCU010000000
VERSION     DASYCU010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592951"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VGM24_13120"
     CDS             complement(<1..329)
                     /locus_tag="VGM24_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156373.1"
                     /translation="MTRAKNRQLLGAVTRKQCGEAGLAAVLIALILALHFKKELYVVL
                     AFVFTLITLTIPLILYPFALLWFGLAKWLNRISTTVMMALVFFLVVVPVGILRKRMGR
                     DRMKIKE"
     gene            complement(310..2394)
                     /locus_tag="VGM24_13125"
     CDS             complement(310..2394)
                     /locus_tag="VGM24_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY4156374.1"
                     /translation="MNKREPGTPAPGRKYSKKRLLWFKVIGLFIPLLVLFFLEISLRL
                     FHYGYNPALFIEYPPDHRYLYFNPEASKKYFADQAIATTGNIEAFKKEKDENTTRIFV
                     LGESTTIGYPYFHNASFHRWLQFRLTHSIPGRNFEIINCSLTAVNSYTVLGFARQLVD
                     YQPDAVLIYTGHNEYYGALGVGSTDRIGGNVHLVNTLIWLRGLKLVQLMTNGYGKLVG
                     LFGKKKPIGEKTRMELMVQDRQIPYGSKLFNRGITQFESNMEATLKCLSGHHIPVFIS
                     NLVRNEKDLKPFISIPPDRVRFPSFQENLQAGMKAFEEGNFTQARKYLEEAEKTYPLH
                     AACNYYLGKLAWRQGNFEEAQAFFSKAADLDALRFRAPGRLNEIISGLCGKYPDTHLV
                     DSRSVFESRADHRIIGDKLILEHVHPNLLGYALLSDAFYGAMKKAGIFYIAPDREISF
                     EQLRERMPITLLDSLTGAVRIERLRKTWPFSQTQAGEPEEKDSMSSAAEEENLAYSVA
                     FKKLSWPQANETLYNYYIAHQEFRKAKTVVETLVLEYPQEEAYYEKAANLCGQLKEDE
                     EAVFYFRKAFDLSPSFEKARFLFVLYLKMDRPEESVPYLDYAIRNGNDSRLPAIKRLV
                     GEISGLRKSYAADSSNLALPNEIAGKYAQMGNPDAAMKYLNKVLHSDSKNRAALALRA
                     KINNGKDDQGKK"
     gene            complement(2463..>3552)
                     /locus_tag="VGM24_13130"
     CDS             complement(2463..>3552)
                     /locus_tag="VGM24_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="G-D-S-L family lipolytic protein"
                     /protein_id="HEY4156375.1"
                     /translation="LGDFEKDGRALIHSFKERPTHPRIILLLPLPCFLKDTTQINDRI
                     IVSQIIPALRQLAYDENIEVLDMHSLFMDRESGMPDKIHPDGPGSHLIAERLFHLIAQ
                     TGDWQFDIFRALHHDFKSGSFNGYASANFTLDGKACLVVKPKWTAAGRPWIWRARFWG
                     HEPQTDIALLERGFHLVYCDVSELFGNEEAVRCWNDFYDLLHRAGLGKKAVLEGMSRG
                     AVYALNWAAVNPDKVNGVYIDNPVLDLKSWPAGLGRQPASPEELEILKADYRLKGEDD
                     LRRFKGSPVDNIPQIASGHYPILILCADADDAVPPEENTLLFERKFRALQGDVWVLHK
                     PGFKHHPHSLPDPTPIVDFILKATAIDQ"
BASE COUNT          866 a          936 c          850 g          900 t
ORIGIN      
        1 aattccttta tcttcatccg gtccctgccc atcctctttc tcaaaattcc taccggcacc
       61 accaccagaa aaaatacaag ggccatcatg accgtcgtac tgatccggtt cagccatttc
      121 gccaaaccaa accagagcag cgcaaaagga taaagaataa gcggaatggt aagggtgatg
      181 agcgtaaaca caaaagccag caccacatac agctcctttt taaaatgcag agccaggata
      241 agggcaatca acaccgccgc caggcccgct tccccgcatt gtttccgggt gactgccccc
      301 aataattgtc tattttttgc cctggtcatc tttcccgtta ttgattttag cccgcaaagc
      361 aagcgcggcc cggtttttac tatctgaatg taacacctta ttgagatatt tcatggcagc
      421 gtccggattt cccatttggg catactttcc ggcaatctca tttggcaggg ccagattact
      481 gctgtctgct gcataagatt tccggagacc gctgatctct ccgaccagtc ttttaatggc
      541 gggcagtctg gaatcattcc cgtttcgtat ggcataatcc aggtagggaa cagattcctc
      601 cggtcggtcc attttaagat aaaggacaaa aaggaacctc gctttttcga aagaaggcga
      661 aagatcaaat gctttcctga aataaaaaac agcctcctca tcttccttca gctgaccgca
      721 gagatttgcg gctttttcat aatacgcttc ctcctgcggg tactccagta cgagtgtttc
      781 gaccaccgtt tttgccttac ggaattcctg gtgagcgata taataattat agagtgtttc
      841 gttcgcctgc ggccagctca gcttcttaaa agcgaccgaa taggccagat tttcttcctc
      901 tgcagcggaa gacatgctgt ctttttcctc cggctctccg gcctgtgtct ggctgaaagg
      961 ccaggttttc cggagccgct cgattctgac ggccccggtt aatgaatcca gaagggtaat
     1021 gggcattcgt tcccgcaact gctcaaaaga aatttcccgg tccggtgcta tgtaaaatat
     1081 tccggccttt ttcatggccc cgtaaaaggc gtctgataga agcgcatagc caagcaggtt
     1141 aggatgaacg tgctcgagaa tcagcttatc gccgataatc cgatgatctg cccggctttc
     1201 aaaaacagac cggctgtcca ccaggtgcgt atcaggatat ttgccgcaca gtccggaaat
     1261 tatttcattg agccgtcccg gtgcgcggaa ccgcagggca tccagatcgg ccgccttcga
     1321 aaaaaacgcc tgggcttctt caaaattccc ctgccgccag gccagtttcc ccaggtaata
     1381 attacaggct gcatgcaacg gataggtttt ttccgcctct tccagatatt tacgggcttg
     1441 ggtaaaatta ccctcttcaa atgctttcat gcctgcctgc aggttctcct gaaaggacgg
     1501 aaaccggaca cgatctggcg gaatactgat aaagggtttc aggtcctttt cattgcggac
     1561 gagattactg ataaagaccg ggatatggtg cccgctcagg catttcaggg tggcctccat
     1621 attcgattcg aactgggtga taccgcggtt gaacaacttc gaaccatagg gaatctgccg
     1681 gtcctgcacc atcagttcca tgcgtgtctt ttcccctatg ggcttttttt tgccgaaaag
     1741 gcccaccagc ttcccgtaac cgtttgtcat cagttgtacc agcttcagcc cgcgcaacca
     1801 aataagcgta ttgaccaggt gaacattccc gccgatccgg tccgtggagc ccacacccaa
     1861 tgcaccataa tattcattgt gcccggtata gatcagcacg gcatcgggct gatagtccac
     1921 cagctgccgg gcaaaaccca gaacggtata ggagtttacc gcggtgagcg aacaattgat
     1981 gatctcaaaa ttccggcccg gtatgctgtg ggtaaggcgg aattgcagcc agcgatgaaa
     2041 ggaggcattg tgaaaatagg gatagccgat ggtcgttgat tcccctaaaa caaaaatgcg
     2101 ggtcgtattt tcatcctttt cttttttgaa tgcctcgata ttgccggtgg tggcaatggc
     2161 ctggtcggca aaatattttt tagaggcctc cgggttgaag tacaggtagc ggtgatccgg
     2221 cggatattct atgaaaagcg ctgggttata cccgtaatga aaaagccgca gggaaatttc
     2281 caggaagaat aaaacaagca agggaatgaa gagaccgatc actttaaacc agagcaatct
     2341 ttttttggag tatttccttc cgggggccgg cgttcctggt tctcttttat tcatacggct
     2401 gtattcgttc tgctgtcttc ccttccggtt ccccggaaac agaagatacg cattcccgtc
     2461 gtttattgat caatggctgt cgctttcaaa ataaaatcaa caattggagt cggatccggc
     2521 aggctgtggg ggtggtgttt aaatcccggt ttatgtaaaa cccagacgtc tccctgcagc
     2581 gccctgaatt tccgttcgaa caaaagagta ttctcttccg gaggcaccgc atcatctgca
     2641 tccgcgcaaa ggatcaggat cggataatga ccggacgcta tctgcgggat gttatcgacc
     2701 ggacttcctt taaaccgccg gagatcgtcc tctcctttaa ggcgataatc tgctttaaga
     2761 atttccagtt cctccggaga agcgggttgt cttcccagtc ccgcgggcca gctctttaaa
     2821 tccaaaaccg gattgtcaat atacacccca ttcactttgt ccgggttgac cgctgcccag
     2881 ttgagcgcat agaccgcacc ccggctcatt ccttccagaa ccgctttctt tcccagcccc
     2941 gcgcggtgca gcaggtcata gaaatcattc cagcatctca cggcttcctc attcccaaat
     3001 aattccgaca catcgcagta aaccaggtgg aaaccccgct ccagcaaggc aatatccgtc
     3061 tggggctcat gcccccagaa gcgggcgcgc cagatccagg gtcgtccggc ggcggtccat
     3121 ttgggtttta ccacgaggca ggctttcccg tccaacgtaa aattcgctga tgcataaccg
     3181 ttaaatgaac cggatttgaa atcatgatgg agagcccgaa aaatatcgaa ttgccagtct
     3241 ccggtttgtg caatcaggtg aaaaagtctt tcggcgatca ggtgggaacc cggcccgtcc
     3301 ggatgtatct tgtccggcat tccggattcc ctatccatga acagggaatg catatccagc
     3361 acttcgatgt tttcatcata ggccagctga cggagagccg gaatgatctg tgaaacaatt
     3421 atccggtcat tgatctgcgt agtatctttc agaaagcagg gcaatggcaa taataagatt
     3481 atacgcgggt gcgtgggccg ttctttgaaa gaatggataa gggcgcgacc gtctttttcg
     3541 aaatcaccga ga
//
LOCUS       DASYCU010000653         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4199486, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000653 DASYCU010000000
VERSION     DASYCU010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4199486"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGM24_13135"
     CDS             <1..648
                     /locus_tag="VGM24_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEY4156376.1"
                     /translation="FWGLIIGYWLLLKFTSAPGFPTGDLTMQGNFTSYVDRLIMPGKL
                     YLGIHDPEGLLSTIPAISTGLLGILTGNLLKNGNMSSRKKARWMAVAGIIFLVLAQIW
                     NLDFPINKNLWTSSFVLHVGGISLLLMAFFYYVIDILGHKRWAFFFRIIGMNSILIYI
                     SGHFINWGYMTNSFFHWLGQLVGDPFGAVVLAACLIFVKWLFLYFMYRKRVFLRV"
     gene            complement(689..2908)
                     /locus_tag="VGM24_13140"
     CDS             complement(689..2908)
                     /locus_tag="VGM24_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEY4156377.1"
                     /translation="MRRIYLLLSGLLLLNANLPAQTKPITGFTPSSSLTESRTEAAFD
                     QSISRTSIGNTIRELSEKPHHLGSPGSREVAEKIRQKLTEYGFDTRMDVYQVLFPTPK
                     KRILEMTSPGIYRAVLKEPALKEDASSGQEGQLPTYNAWSNDGNVTAPLVFVNYGLPA
                     DYDELARMGVDVKGKIVIAKYGMSWRGIKPKVAQEHGAVGCIIYSDPKEDGYFQGDVY
                     PKGPYKNEYGVQRGSVMDMPIYPGDPLTPGVGATKDAKRIPSHTEAPNLLKIPVLPIS
                     YHDAKPLLEALTGPVAPESWRGALPITYHVGPGKTTVHLQLEFDWKLVPCYDVIAKIK
                     GSDFPDEWVIRGNHHDAWVNGAQDPISGQASLLEEAKAIGELVKSGWKPRRTLVYCAW
                     DGEEPALLGSTEWAEDHDSLLKEKAVLYINSDGNGRGFLYAGGSHALEGLVDEVAKEI
                     TDPQTRVSVYERLKAHKIAGAANPKQAKEEMDKKSLSLDALGSGSDFSSFLQHLGVPS
                     LNIGYGGEDDGGEYHSIYDSYDNFIRFKDPGFQYEALLSKTAGHLTLRMANADLLPFD
                     FRSLLTKIKTYSTELQTALNNLRENTSIENEVLKGNYLTLAADTAKPFFSPKPKAEVP
                     FIDFSPLLNAIPVLEKAVEHAHDAWENAKLSKGNHDALNQLLYRAEQQLLLENGLPKR
                     PWYKHVLYAPGLYTGYGVKTMPGIREAIEQRDFREAELEIKRTAASLLKLSGYLNNM"
     gene            3021..4046
                     /locus_tag="VGM24_13145"
     CDS             3021..4046
                     /locus_tag="VGM24_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY4156378.1"
                     /translation="MNTFNPEKVRQFFFLAVLVLMGALLFSKLQTFLPSLLGAVTLYV
                     LLGKYMKRLLTRKWKAWTAALLLMLLSFLIILLPVFFLINTVSSNLSYAISHVQELTS
                     SLLVFLRNVESRIGFHFLNEETVRRLSEMAIQELPLILGATFNSVLTLFMMYFLLYFM
                     LTGCEQMEAWLDDILPLRRDNQVKVQQEVSRMVISNAVGIPLIAILQGIVGLIAYLVL
                     GVNQPFLWFALTCFTSMVPILGSALAYVPLSIVLFAGGETAKGVIILIYGLVVIGTTD
                     NVFRFWLQRKIGNVHPLITVFGVLVGVPLFGFIGLIFGPLLLSLFILLMRIYHVEFIA
                     PDKRIAD"
BASE COUNT          890 a          993 c          988 g         1189 t
ORIGIN      
        1 ttctggggtt tgatcatcgg ttactggttg ctgttgaaat tcacttcggc tcccggtttt
       61 ccaacagggg atcttaccat gcagggaaat tttacgtcgt acgtagatcg tttgatcatg
      121 ccgggcaaac tgtacctggg cattcatgat cccgaaggcc tgctttcaac cataccggca
      181 atcagtacgg ggttactggg tatcctgacc gggaacctgc tgaaaaatgg caacatgtct
      241 tcccggaaga aagcccgatg gatggctgtt gccggaatta tttttcttgt gcttgcgcag
      301 atctggaacc tggattttcc cattaataaa aacctgtgga caagctcgtt tgttttgcac
      361 gttggcggca tcagcctgtt gctgatggca tttttttact atgtcatcga cattttaggt
      421 cataaaagat gggcgttctt cttcaggata atcggcatga actccatatt gatctacatc
      481 tccggacatt ttataaactg gggatacatg accaacagtt tcttccattg gctcggtcag
      541 ctggtcggag atcctttcgg tgccgttgtg ctagcagcct gccttatttt tgttaaatgg
      601 ctattcctgt attttatgta ccggaagaga gtctttttgc gggtctgaca ttataaattt
      661 tgagcaggcg gcaaattaat tttcacattt acatattatt caggtatcca ctcagcttga
      721 gcagggaagc ggcagttcgt tttatttcca gctcagcttc cctgaagtct ctttgttcaa
      781 tggcttcccg gatgccgggc atggttttta ccccatagcc ggtgtagagc cccggtgcgt
      841 atagcacatg tttgtaccag ggtcttttcg gaaggccgtt ctccagcagc aactgctgct
      901 ctgcccggta cagcaattga ttcaacgcat catgattccc tttgctgagt ttcgcatttt
      961 cccaggcatc atgagcgtgt tccacggctt tttcgagcac aggaattgca ttcaggagtg
     1021 gtgaaaaatc tataaaagga acctccgctt ttggcttcgg cgaaaagaaa ggttttgccg
     1081 tatctgctgc cagggtcagg taatttcctt tcagcacctc attctcgatg ctggtgtttt
     1141 cgcgcaaatt gttcagggca gtttgcaatt ccgtgctgta tgtttttatt ttagtcagca
     1201 ggcttctgaa atcaaaagga agcagatcgg cgttggccat ccggagtgtc agatgtcccg
     1261 ccgtttttga aagcagggct tcatattgaa agcccggatc tttgaagcgg ataaaattat
     1321 cgtaggaatc atagatggaa tgatattccc ctccatcatc ttccccgcca taaccaatat
     1381 tcagcgaagg gacgcccaga tgctgaagga aagaagagaa gtctgatccg ctgcccaaag
     1441 cgtccagtga caatgacttt ttgtccattt cttctttagc ctgttttggg tttgcggcac
     1501 cagctatctt atgcgctttc aatctttcat aaacactcac ccgtgtttgc ggatccgtaa
     1561 tttccttggc cacttcatcc acaagccctt ccagggcatg cgacccgccg gcgtaaagaa
     1621 atccgcggcc gttgccgtct gaattaatat acagaacagc tttctctttt aaaagtgaat
     1681 catggtcttc ggcccattcg gtggaaccaa gcagggcagg ttcttctcca tcccaggcac
     1741 aataaaccag cgtacgcctg ggtttccagc cggattttac aagctcgccg atggcttttg
     1801 cttcttctaa cagcgatgcc tgaccgctaa ttggatcctg ggcgccgtta acccaggcat
     1861 cgtggtgatt gccccggatc acccattcat cggggaaatc agatccctta atctttgcaa
     1921 tcacatcata acagggaacg agtttccaat cgaattccaa ttgtaaatga acggtcgttt
     1981 taccgggtcc cacatggtag gtaatgggca gggcgcctcg ccagctttcc ggagcaacgg
     2041 gaccggtcag cgcttccagt aatggtttgg cgtcgtgata actgatgggc aataccggta
     2101 tcttcaagag gttgggcgct tcggtatgcg agggtattct tttggcgtct ttcgttgcgc
     2161 cgactccggg cgtgagcgga tcgccgggat agatgggcat gtccataaca ctgccgcgct
     2221 gcacgccata ttcattttta taaggacctt tcggatacac atctccctgg aaatagccat
     2281 cctctttcgg atcagaataa ataatgcagc cgacggcgcc atgttcctgc gctactttgg
     2341 gtttgatccc gcgccagctc attccatatt ttgcgataac gatctttcct ttcacatcga
     2401 ctcccatccg ggccagttca tcgtaatcgg ccggaagccc ataattgaca aacaccaatg
     2461 gcgccgttac attgccgtca ttactccagg cattgtacgt gggaagctgg ccttcctgcc
     2521 cgctgctggc atcttccttc aaagcgggtt ctttcaatac agcccggtaa atgccgggtg
     2581 aagtcatttc gagaattctc tttttaggcg tcggaaacag gacctgataa acatccatcc
     2641 gggtatcaaa gccatattcc gtgagtttct gcctgatttt ttcagccacc tccctgctgc
     2701 cgggtgaacc cagatgatgg ggtttttcgg ataattcgcg gatggtattt ccaatgctcg
     2761 tcctgctgat cgactggtca aatgcagcct ctgttctgga ttcagtcaaa ctggaagatg
     2821 gagtaaatcc ggtgatcggt tttgtttgcg cgggcaggtt tgcattcagc agcagaagac
     2881 cggagagcag gagatatatt cttctcataa atttttcgct tatcttaaaa ttacggcaaa
     2941 atcgggtatg aaagcatttt gcctgtgcgc gcagaattca acggattact ctttgtacct
     3001 tgtaggtaaa atcgcgggcc atgaacacct ttaatccgga aaaagtaagg cagttctttt
     3061 tcctggcagt ccttgtgctg atgggggccc ttcttttttc aaaactgcaa accttcctcc
     3121 cctcgctttt gggagcggtc acgctttatg tgctcctggg aaaatacatg aaaagattgc
     3181 tgacccggaa atggaaagcc tggacggccg ccctgcttct gatgctgctg tctttcctga
     3241 tcatcctgct gccggtattc tttctcatca atacggtcag ttcaaacctg agttatgcca
     3301 tcagtcatgt ccaggaactg accagctcat tgctggtttt tttgcggaat gtggaatccc
     3361 ggatcggatt tcattttctt aatgaagaaa cggttcgcag gctgagtgaa atggctattc
     3421 aggaacttcc gctaatcctg ggggcgacct ttaattcggt gttgaccctg ttcatgatgt
     3481 acttcctgct ttattttatg ctgactggct gtgagcaaat ggaagcctgg ctggatgata
     3541 tcctgcccct gagaagggac aaccaggtga aagtgcagca ggaagtaagc agaatggtca
     3601 tctccaacgc cgtgggtatt cccctgatcg ccattctgca gggtatcgtt ggtctgatcg
     3661 cttacctggt gcttggggtg aaccagcctt ttctctggtt tgcgcttacc tgctttacct
     3721 ccatggtccc catactgggt tctgcccttg cctatgtacc gctttctatt gtattatttg
     3781 ccggcggtga aacggcaaag ggagtgatca tcttaattta cggactggtc gttatcggaa
     3841 caacggataa tgtgttccgg ttttggctac aacgaaaaat agggaatgtg catcccctga
     3901 tcacggtttt tggcgtgctc gtgggcgttc cgctattcgg gttcatcgga ctcattttcg
     3961 gaccgctgct gctttccctg ttcatcctgc ttatgcgcat ctatcacgtt gaatttattg
     4021 caccggataa gcgaattgcc gattagtgcc gaatgcaggc
//
LOCUS       DASYCU010000654         7266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_431490, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000654 DASYCU010000000
VERSION     DASYCU010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431490"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGM24_13150"
     CDS             <1..289
                     /locus_tag="VGM24_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156379.1"
                     /translation="GKALAELRGTKNQTLVAATQNRGDLKLFELRKSQPLIPVTGQSM
                     DFILSLSSGQKRKQELYYGASFLSQSGRYLKRTSQVTGIQKKDKSGNWVDF"
     gene            complement(309..497)
                     /locus_tag="VGM24_13155"
     CDS             complement(309..497)
                     /locus_tag="VGM24_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156380.1"
                     /translation="MKTISTLIYLLVIVSICAASAVLYLKQEYNISALLTVAWIISLT
                     VWIKANGILEDKKSETAQ"
     gene            860..1393
                     /locus_tag="VGM24_13160"
     CDS             860..1393
                     /locus_tag="VGM24_13160"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY4156381.1"
                     /translation="MIDTIFCLVLLMAVIKGWRSGLILALFSVICCLLGLAAAVKLSA
                     VVAAHLESGLKLSSRWLPVIAFVIVFVTVVILVRWAANLIEKGVKMLFLGWLNKLGGI
                     LLFILLYISIYSVALFYGTRVSIISPEAVKASHVYPLIAPWGPSVIRFIEKFIPFGQD
                     MFAVLETFFGKIARHMN"
     gene            1564..2340
                     /locus_tag="VGM24_13165"
     CDS             1564..2340
                     /locus_tag="VGM24_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY4156382.1"
                     /translation="MQYEIKQVDKFRFIEEGEGQPLVLLHGLFGALSNFVDLVEYFRK
                     HYKVVVPMLPLFDLDILHTSVGGLAKFVHKFIEIRNYRNVHLLGNSLGGHVALIYVLK
                     NPERIKSIILTGSSGLFESAMGDTYPKRGDYDYIKKKTELTFYDPKIASKELVDEVYE
                     IVNNRIKAIKIIALAKSAIRNNLGEELNQIMQPALLIWGSNDSITPPFVGREFQRLIP
                     HSELHFIDKCGHAPMMEVPAEFNEILHQFLTKLNEPAAVA"
     gene            2375..3037
                     /locus_tag="VGM24_13170"
     CDS             2375..3037
                     /locus_tag="VGM24_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY4156383.1"
                     /translation="MLNQEILSISIPPLHPDDSVLLAREAMEDHHVAHLPVILDQKLL
                     GIISEDSLLNSESDSMPLTRLQSSFSGAAVQGSNHIIETIQLLNEFNLTLVPVINKES
                     DYLGAITALELLRNAGILIGAGDPGALVVLELDKVNFSFAEISKLVETNDAQITQLNT
                     YFDAIHSTFYITLRINKKEISDIIATFQRYEYKIKHYFGEEHYDNELRSNYNHLMNYL
                     SI"
     gene            3079..4536
                     /locus_tag="VGM24_13175"
     CDS             3079..4536
                     /locus_tag="VGM24_13175"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY4156384.1"
                     /translation="MIRKPGKYLVLIFALLGFLTGRANAIRSGKEWPACRSDSLPPLP
                     QNHLFKIGRINIHGNRVTRGYIIERELPFKTGDSLMLSDITESFVKIRERLINTRLFN
                     EASISLDEFRGYTIDIRIDVKERWYIFPLPYVRPVDRNFTAWADQHYSLKRFDYGLKY
                     SQYNFTGRNDFLRIWLITGYTREVELAYDHPYGGRELKHGFGGGFQYAGQQELNLATV
                     SNKQVFVNRDSLPYTGKFLREQLNFSLRYYYRPGLRTRHFVKLSFNDVRIDSAVLNYN
                     SNYFSGNRRNVFYPELSYSISYNNIDYVPYPTRGFYFEGGLIRRGLTADMNMWQLNAK
                     SSEAFSFAPKMYFVTDNLALIRVPFNQPFYNKQLLGYNDFYMRGLERYVVDGVACITA
                     RNTVLRELTHFSIPFLRGTSHDHIPFRIYAKAYLDLGYVYDKYDNINSLVNKGLYSGG
                     FGVDVVTFYDFVFRAEFSMNQLGEKGVFFHIRNDF"
     gene            4653..5531
                     /locus_tag="VGM24_13180"
     CDS             4653..5531
                     /locus_tag="VGM24_13180"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY4156385.1"
                     /translation="MKVAIYSRVIDENERVEVQQLLDELERMQIRVMIYQPFYDQISG
                     AFRFPGNMIFFRDSGQLDDSVDFLISLGGDGTLLDTISLVRDKNIPVIGINFGRLGFL
                     ASIGKGNMLEAVKALVNRTFVADKRSLIHLDANKALFGEVPYGLNEFAIHKTDTSPMI
                     KIHTYLNGEFLNTYWADGLIVSTPTGSTGYSLSCGGPLVFPESGSFVINPVAPHNLNV
                     RPIIVPDDNIISFEVEGRAENFICALDSRKEIVDKNVQLAVRRESFNISLVRLNENNF
                     LQTLRNKLSWGLDRRN"
     gene            5624..6493
                     /locus_tag="VGM24_13185"
     CDS             5624..6493
                     /locus_tag="VGM24_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HEY4156386.1"
                     /translation="MKQILITGMGLFSFLFQAGAQDRYVPEDNSLVHEGEFGISAGAA
                     HYFGDLNPDYHFNRPKPAIGIFFRKQFGNYIAIRVAGHYAQVGYSDIYNTQNEFEHRR
                     NLSFNSNVWELGVQGDFNFFRFIPGDGYHRATPYITFGVGMFSFDPYAYYQGQKIFLR
                     ELGTEGQGTAAFPTRKPYNNISAYIPLGVGYKYNLNPKMNIGFEVVYRFTTTDYLDDV
                     STTYAGIENFPTLPGGDPSIAAIMQDRSNETGTPIGIKGKQRGYSNQNDGYVFAEIMF
                     SFNITSYHCPTAK"
     gene            6570..>7266
                     /locus_tag="VGM24_13190"
     CDS             6570..>7266
                     /locus_tag="VGM24_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY4156387.1"
                     /translation="MESLKSKLDPVRLPTHIAIIMDGNGRWAKVQGQDRLFGHYHGVE
                     SVREVVEGCAELGIGYLTLYAFSTENWDRPPGEVSGLMELLVQTIRKEVPTLNKNNIR
                     LQVIGNTHMLPDSTLEALDEAILETATNSGLNLIMALSYSSRWEMGEAARRLGRDVKA
                     GKLDPEAITEQTFEKYLCTRGIPDPELMIRTSGEYRISNFLLYQLAYAELYFTDTRWP
                     DFRKENLYEAILDF"
BASE COUNT         1922 a         1631 c         1794 g         1919 t
ORIGIN      
        1 tggtaaggcg ctggctgaac tgcggggaac aaaaaaccag acactcgttg ccgctacgca
       61 aaaccgcggg gatctgaaac tatttgaatt gcgaaaatcc caaccgctga tcccggtcac
      121 cggtcagtca atggatttta ttcttagttt gagcagcgga caaaaacgca aacaggaatt
      181 gtattacggg gcttccttcc tgtcgcagtc cggtcgttat ctgaaaagaa ccagccaggt
      241 aacggggata caaaaaaaag ataagtcggg aaactgggta gatttttaaa tctggcggtc
      301 cgccgatctt actgggcggt ctccgatttt ttatcttcca gaatgccatt ggccttgatc
      361 caaacggtga gtgaaatgat ccaggcaacg gttaacaggg cggaaatatt atattcctgt
      421 ttcaggtaaa gaacggcact ggccgcgcag atggatacaa tgaccagaag gtaaatcagg
      481 gtagaaatcg ttttcatttt gtacgcttta ggtatgtaaa aaaataaatt acggattgat
      541 cggggctgat cacgaaattg gcacgtaatt cagaggaatg gaaaacgatt aaaaagcaaa
      601 actaaaattc agaagggatg attggaggaa ttatcaggag gagattgggt acagatccgg
      661 ggttgataaa acaaagatta aacacagaat tcaaaaatgc aagggaagag aagaattttt
      721 ttgatttttt gttgccggag tttcggcgcc ggctcaccgg caggcagatt cccaaattga
      781 gaattggcgg aacgccattt gaaatgaacc ggcgtccggg tgaatcgtta atccctacat
      841 ttatacctgt attattctta tgatagacac gatattctgc ctggtcctgt taatggccgt
      901 cataaaaggt tggagaagcg gcttaatact ggcgctgttt tcggtgatct gctgcctgct
      961 gggacttgcc gccgctgtaa aattatcggc cgtcgtcgct gcccacctgg aatcaggcct
     1021 gaagctgtct tcccgctggc tgccggtgat tgcatttgtt attgtatttg ttaccgtggt
     1081 cattttagtc aggtgggctg ccaatcttat tgaaaagggg gtaaaaatgc tgtttttagg
     1141 atggcttaac aaattgggag gtattttact attcatttta ttatatattt cgatttacag
     1201 tgtggccctg ttttatggaa cgcgggtttc catcatatcg cctgaagcgg taaaggcatc
     1261 acatgtatat cccttgattg ccccctgggg accttcagtg atccgtttta tagagaaatt
     1321 catccccttt ggtcaggata tgtttgcggt gctcgaaaca ttttttggta aaattgcccg
     1381 tcatatgaat taaccggcat tcagcgttcg ggccggcgga aagggccgga gagatcccga
     1441 aagcattata aacgacttat cgtacatttg taatgaaatc attgttagcc ttttgaaaga
     1501 gatgattttt gcagtaaatc gtttatttta gcgaatattt attggattta ttttaattgt
     1561 gtgatgcagt acgagatcaa acaggtggat aagtttaggt tcattgaaga gggcgaaggg
     1621 caacccctgg tattgctgca tgggctattt ggggcactca gcaatttcgt tgacctggtc
     1681 gagtatttca ggaagcacta taaagtagtg gttcccatgc ttcctttatt tgatctggat
     1741 atcctgcaca cttcggttgg aggattggct aagtttgtcc ataagtttat cgaaatccgt
     1801 aattaccgca acgtccatct cctgggaaat tcgctgggag ggcatgttgc cctgatctat
     1861 gtgctgaaaa atcctgaaag gataaaatcc attatcctga cgggcagttc gggccttttt
     1921 gagagcgcta tgggagacac ctacccgaaa cggggagatt atgattatat caagaagaaa
     1981 acggagctca cgttttatga tccgaaaatt gcttcaaagg aactggtaga tgaggtatat
     2041 gaaattgtta acaaccggat caaagccatc aaaattatcg ccctggcaaa aagtgcgatc
     2101 agaaataatt taggagagga actcaatcaa atcatgcagc cggcgttatt gatctgggga
     2161 agcaacgaca gcattactcc gccctttgta ggcagggaat ttcagcgttt gatcccgcat
     2221 agcgagctgc attttatcga caaatgcggc catgcgccca tgatggaggt tccggccgaa
     2281 ttcaatgaaa tattacacca gtttctgacc aaattaaacg aacctgcagc agtggcatga
     2341 gacagatagg atcttatcac taaaacccct tgctgtgtta aatcaggaaa ttttatccat
     2401 tagtattccg ccgcttcacc cggacgacag cgtcctgctg gccagggaag ccatggaaga
     2461 tcatcatgta gcccatctcc cggtcattct ggatcaaaaa ctgctgggaa taatcagtga
     2521 ggacagcctg ctgaattcgg aatccgattc gatgcccctt acccgcctgc agtcttcctt
     2581 ttccggagcc gctgtccagg gctcaaacca tatcatcgaa accatccagc ttctgaatga
     2641 attcaacctg accctggtac cggttatcaa taaggagtcc gattacctgg gagctatcac
     2701 cgcgctggaa ttgctgagga atgcgggaat actgatcggt gccggggatc ccggtgcgct
     2761 ggtggtgctt gaactggata aggtcaattt ttcttttgct gaaatcagca agctggtgga
     2821 aacaaatgac gcgcagatca cccagctcaa cacctatttt gatgccatcc attctacctt
     2881 ttacatcact ctccggatca ataaaaagga aatctcggac atcatcgcta cttttcagcg
     2941 gtatgaatac aagatcaaac attattttgg cgaagagcac tatgataatg aattgcgcag
     3001 taattacaat cacctgatga attatctgag catttaatcc atagctcata ttatccatgt
     3061 aaagaccggg aaaattctat gatccgaaaa ccgggtaaat atcttgtact gatctttgca
     3121 ttactcggat tccttaccgg aagggcaaac gccatccgtt ccgggaagga atggccggcc
     3181 tgccggtcag acagtcttcc tcctctgcca cagaaccatt tatttaagat cggaaggatt
     3241 aacatccatg gcaaccgggt gacccggggt tatatcatcg agcgggaact tccgtttaaa
     3301 accggggatt ccctgatgct ttccgatatc acggagtcct ttgtgaagat ccgcgagcgc
     3361 ctcatcaata cccggctctt taatgaagcc agcatatccc tcgatgaatt caggggctat
     3421 acgattgaca tccgcatcga cgtaaaagaa cgctggtata tttttccttt gccttatgtg
     3481 cgcccggtcg accggaattt tactgcctgg gcggatcagc actatagttt aaagcggttc
     3541 gattacgggt tgaaatattc gcaatataat tttacgggac gcaatgattt tttgcggatc
     3601 tggctgatca cggggtatac ccgcgaagta gaattagcct acgaccaccc ttatggcggc
     3661 agggagctga aacatggatt tggcggaggt ttccagtatg ccggacagca ggaactgaac
     3721 ctggcaacgg tctccaacaa gcaggtgttc gtaaatcgcg acagcctgcc ctataccgga
     3781 aaatttcttc gggaacagct gaatttttcc ctgcggtatt attaccgtcc ggggctacgc
     3841 acccggcatt ttgtaaaact gagtttcaat gatgtccgga tagacagcgc cgtattgaat
     3901 tacaattcca attatttctc cggcaaccgg cgaaatgtct tttatcccga actttcctac
     3961 agcatcagtt ataataatat tgactatgtt ccttacccca cccggggatt ttatttcgaa
     4021 ggcggattga tccgtcgcgg attaacggcg gatatgaata tgtggcagct gaatgcaaag
     4081 agcagcgaag cgttttcctt tgcgcccaaa atgtattttg ttacagataa cctggccctg
     4141 atccgggttc cctttaacca acctttttat aacaaacagc tgctgggata taatgatttt
     4201 tatatgcggg gcctggaaag atatgtggtg gatggagtag cttgcatcac ggcgcgcaat
     4261 accgtgctcc gggaactgac ccatttcagt attccctttc tccggggcac ttcgcacgat
     4321 catattcctt tccgtatcta tgcaaaagct tatttggatc tgggttatgt gtatgacaaa
     4381 tatgataaca tcaattcgct ggtgaacaaa ggactttatt ccggcggttt tggcgttgat
     4441 gtggtcactt tttacgattt tgtattccgg gccgaattca gcatgaacca attaggagaa
     4501 aaaggagtat tttttcatat cagaaatgat ttttaaagag attataaaag ggaaatgaaa
     4561 tcaaagaatg aatccttcac cgcagcattt gcattttcac ggtagatcct gcattgatgg
     4621 gaacattctg taaataaaaa aatatacaag ggatgaaagt agcgatatac agccgggtca
     4681 ttgacgagaa tgaacgggtt gaagtgcagc agctccttga cgagctggag cgcatgcaga
     4741 tccgggtcat gatctatcag cctttttatg atcagatctc cggggctttc cgctttcccg
     4801 ggaacatgat ctttttccgg gattccggtc agctggacga ttcggttgac tttctgatca
     4861 gcctgggcgg agacggcacc ctgctcgata ccatttccct ggtcagggat aaaaatattc
     4921 cggtgatcgg gatcaatttc gggcggctgg ggttcctggc cagtatcggg aagggaaata
     4981 tgctggaagc cgtaaaagcg ctcgtaaacc ggacctttgt ggcagataag cgatcgctga
     5041 tccacctgga cgccaataaa gccctttttg gggaagtgcc ctacgggttg aatgaatttg
     5101 ccattcataa gaccgatact tcgcccatga tcaagattca tacttacctc aatggtgaat
     5161 ttctaaatac ctactgggcg gatggattga tcgtttccac gccgacgggt tccaccggtt
     5221 attccttgag ctgcggcggc cccctggtgt ttccggaatc gggcagtttt gtgatcaatc
     5281 ccgtggcgcc gcataacctg aatgttcggc cgatcattgt gcccgacgac aacattattt
     5341 cttttgaagt ggagggaagg gcggagaatt ttatctgtgc cctggattcc aggaaagaaa
     5401 ttgtggataa aaatgtacaa ctcgcggtca ggcgggaatc ctttaacatc tcactcgtcc
     5461 gcctgaacga aaataatttc cttcaaacct tgcggaacaa gctgtcctgg ggcctggacc
     5521 ggaggaatta acgtatcctt tataagaaaa aacccccaac ttcgttaagc cggctaatgg
     5581 aataactttt tataagcaaa ggattatcta ttattttgcg ggtatgaagc agatattaat
     5641 taccgggatg ggcctgttca gtttcttatt tcaggcgggt gcgcaggacc ggtatgttcc
     5701 ggaggataac agcctggtcc atgaaggcga attcgggatc tcggccggag ctgcccacta
     5761 tttcggtgat ctgaacccgg attatcactt caaccggccg aaaccggcca tcgggatatt
     5821 tttcaggaag caatttggta attatattgc aataagggtg gccggtcatt atgcccaggt
     5881 cggatattct gatatctaca acacccagaa tgagtttgaa caccggcgca acctcagttt
     5941 taacagcaat gtctgggaac tgggtgttca gggtgacttt aattttttcc ggttcattcc
     6001 cggggatggc tatcaccggg ctacaccgta tatcacgttt ggcgtcggca tgttcagttt
     6061 tgacccctat gcgtattacc agggacagaa gatctttctc cgggaactgg gcaccgaggg
     6121 ccagggtacg gcggcttttc ccacccgcaa gccctataat aatatctcgg cctatattcc
     6181 gctcggcgtg ggctacaaat acaatctcaa tccaaagatg aatatcgggt ttgaagtcgt
     6241 ttaccgcttc accactaccg attatctgga tgatgtgagc acgacctatg ccggtattga
     6301 gaacttcccc acgctgcccg gcggggaccc ctccattgcc gccatcatgc aggatcgctc
     6361 caacgaaacc ggaacaccga tcggcatcaa aggcaaacaa agaggctatt ccaaccagaa
     6421 tgacggctat gtttttgccg aaatcatgtt ttccttcaac atcacctcct atcattgtcc
     6481 tacggccaag taagctcctg atcctgagct aatttacatc cgcactgaat tgcgaaaatt
     6541 gtataccttc gtgcccttca aaaaggagga tggagtctct gaaaagcaag ctggatccgg
     6601 tacgattgcc tactcacatt gccatcatta tggatggaaa cgggagatgg gccaaggtac
     6661 agggtcagga caggctgttt ggacactacc acggggtgga aagcgtgcgg gaagtggtgg
     6721 agggttgtgc tgaattggga atcggatatc tgaccttata cgctttttca accgaaaact
     6781 gggacaggcc gccgggagaa gtgagcggat taatggaact gctggtgcaa acgatccgca
     6841 aggaagtgcc tacgctcaac aagaataata tccggcttca ggtgatcgga aacacgcata
     6901 tgttgccgga ttcgaccctg gaagccctcg atgaggccat cctggaaacg gcaaccaaca
     6961 gcgggctgaa tctcattatg gccttgagtt acagcagccg ctgggagatg ggggaagctg
     7021 cccgccggct gggaagggat gtaaaggcgg gaaagctgga cccggaagcc attaccgagc
     7081 aaacctttga aaaatatctc tgcacccggg gtatccctga tcctgaactg atgatcagga
     7141 ccagcgggga ataccggatc agtaatttcc ttttatacca gctcgcttat gccgaattgt
     7201 acttcaccga tacccgctgg cccgacttcc ggaaagaaaa tctctacgag gccatcctgg
     7261 acttcc
//
LOCUS       DASYCU010000655         6299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2316732, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000655 DASYCU010000000
VERSION     DASYCU010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316732"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2064
                     /locus_tag="VGM24_13195"
     CDS             <1..2064
                     /locus_tag="VGM24_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEY4156388.1"
                     /translation="ARVNLDAKINKYISTSFGLTLREESRFFPTQSAQSIFRMMMRGR
                     PTDPEVWPNGLPGPDIENGQNPIVITTNQTGYDRDKRDYFLGIGKVEINIPWVQGLKL
                     TGSVSLDNQTKRTKTWQTPWYLYFWDHATYEADGKTPLLTKSIRSTFTNPQLSESDAK
                     YLNILTSGFLNYDRTFGPHTVNLMAAVTKETDDEDDFNAYRTNFISPAIDQLFAGGAN
                     QQTIGGSAYDRARLSYFGRAAYNYKEKYLAEFLWRYDGSYLFPEAHRWGFFPGVLAGW
                     RISEEDFFKNAVPFINYLKIRGSWGRMGNDQVYYGGLLKEYQYLATYGFSNYVIGNQG
                     VTTLYETVVPNPNFTWEVTNNTDVGLEGQLMKGKINFEFDYFYNKRNNILWQKQGSTP
                     GSSGIAYLLPPENIGKIQNTGYEFVLGYNGDFANGKFRYSVSVNGGYAKNKILFFDEN
                     PGAPEWQKATGHPFTANGGAAFLAYQYDGVFVDQADIDKTTIDYTGLTNTLRPGDMKF
                     KDVNGDGKINADDQERLNKTGDPTFTGGLNIQLQYSNFDLSILFQGATGGLLYIGTES
                     GDIGNYLQYSYDHQWTIDHPSSVDPRIANRNNTYYAGGAAGNNTYFLRSSDYFRLKNV
                     ELGYNLPSELGKKVGISNLRLYVNGLNLVTWDKMKIWDPESTRGDGQYYPQARIINVG
                     ARVTF"
     gene            2075..3958
                     /locus_tag="VGM24_13200"
     CDS             2075..3958
                     /locus_tag="VGM24_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEY4156389.1"
                     /translation="MKKIFINIILLISGVVLISSCKRDFLNTEPLNAISSDATWKDAG
                     LSQAFVTNIYNGLQNGGFYEQELASLSDEAVFVHPGRGINVINEGTLSPSNLGWQDGT
                     YEWVNMYNYIRPCNLSLENLATAGFDDQELNDRLRGEAHFLRAYYYQQLLRYYGAVPI
                     VDHTYGINEDYSIARNTYEECVNFIVNDCDSAAMLLDGKTLDKGRATSLSAMALKSRV
                     LLYAASDLHDIPTASAKSSVIAGYANPELLGYVSGDRVARWQAAKDAAKAVLDASSGY
                     KLDLTAPASAADAQAYYTSIAMGGGSNGPGIDKAASVELLFERDFSSQLVSGGPGTDG
                     TSVGLDNGPNGYHNWAGNVPIQELVDDYEMMDGSKFDWSNGAEKAAPYKNRDPRLYAT
                     ILFDGADWKPRDKITSNPDPANQIQSGQYQGAGGTTIAGLDTRQSVVENWNASWTGYY
                     MRKFIDPDPAVVDNNTRQYIPWPFLRYTEAVLNYAEACIELGQEAEAVTWLNKIRFRA
                     GMPAITESGSALMQRYQNERRVELAYEEHRYHDARRWMIAPTTLGRPVIDINVVGTLK
                     PGKVAPSPYVHDESIYNYSYTPFSDNGLENRIWVDKMYFRPISRDEVNKNAKLIQNPG
                     YDQ"
     gene            4052..>6299
                     /locus_tag="VGM24_13205"
     CDS             4052..>6299
                     /locus_tag="VGM24_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY4156390.1"
                     /translation="MNHSRSGPFCLLLLLSIIAFSGCTNNPIEKKNTAAPSNTLFTLL
                     QPEKTHVSFSNTLTEGLNTNVLIYEYFYNGGGVALGDVNGDGLTDIYFTGNMTDNKLY
                     LNKGHMEFEDITGSAGVSGRPGPWKTGVTLVDINGDGRLDIYVCYSGKLRGEKRVNQL
                     FINDGNDNQGRPHFSEQAEQYGLADSSYSTQAVFFDYDRDGDLDMLLLNHSITRRTSL
                     DEATIQKLLLENDHLSGLKLFKNDHGHFTEVTAAAGIKSTVLSHGLGAGIADLNGDGW
                     PDIYISNDYLEPDYLYINNKNGTFTDQLQKTLGHTSFFSMGNDIADINNDALPDIFTL
                     DMLPEDNHRQKLLFAPDNYEKIEVNIHSGFYYQYMCNMLHLNNGNGSFSDIAQLAGIS
                     NTDWSWAPLFADYDNDGWKDLYVTNGYLRDYTNMDFLKYMGDALKDRQVMRKDLLALV
                     QEMPSSRVKNYMFRNNGDLTFQNTGKQWGFDTASNSNGAAYADLDNDGDLDLVVNNIN
                     QPAFIYENQSNRLLNNHYLELRLEGSGQNTQGIGAKVTIYLKNKQQYLEQMPARGFQS
                     SVSPVLHFGLGKERTIDSLKIVWQSGKTETLRDIKGDQLLALKEKDALIKPQPVRIAS
                     PVFKEVRSPISFHAVTNEINDFKRQPLLINPLSFQGPCLVKGDVNGDHLEDIYAGGGS
                     GQAGALYIQQKNGQFTRKAEPAFEADKGSEDADALFIDVNGDGFPDLYVASGGYHNYL
                     PDDLLLQDR"
BASE COUNT         1736 a         1550 c         1581 g         1432 t
ORIGIN      
        1 gcccgggtga acctggatgc caaaataaat aaatacatca gtaccagttt cgggctgacc
       61 ctgcgggaag aatcccggtt ttttccaacg caatccgccc agtccatctt ccggatgatg
      121 atgcgcggac ggcccaccga cccggaagtt tggccaaacg gattaccggg tcccgatatc
      181 gaaaatggtc agaacccgat cgtgatcacc accaaccaga ccgggtacga ccgggataaa
      241 agagattatt ttctcggaat aggcaaagtg gaaataaaca tcccctgggt acagggactc
      301 aaactcaccg gatcggtttc cctggataat cagaccaagc gtaccaagac ctggcaaact
      361 ccctggtacc tctacttctg ggatcacgcg acttatgaag cagacggaaa gactcccctg
      421 ctcacaaaaa gcatccggtc aacgttcacc aatccgcagt tgagtgaaag cgatgccaag
      481 tatttaaata tcctgacctc cggtttttta aattacgacc gcacattcgg cccgcatacg
      541 gttaacctga tggcagccgt caccaaggaa accgatgatg aagacgattt caatgcttac
      601 cgcaccaact tcatttcccc tgcgatcgac cagttatttg ccgggggcgc aaaccagcaa
      661 acgatcggag gttctgccta tgacagggcc cgcctgagct attttggccg ggccgcctat
      721 aactacaagg aaaaatacct ggctgaattc ctctggcgct atgatgggtc ctacctgttc
      781 cccgaagcgc accgctgggg atttttcccg ggcgttctgg ccggatggcg gatttcagaa
      841 gaagatttct ttaagaatgc cgttccattt attaattatt taaaaatcag aggatcctgg
      901 ggaaggatgg ggaacgatca ggtctattac ggaggtctcc ttaaggaata ccaatacctg
      961 gccacctacg gcttcagcaa ttatgtgatc ggcaaccagg gcgtgaccac cctgtatgaa
     1021 accgttgtgc ccaatccgaa ttttacctgg gaagtaacga acaatacgga tgtcggcctg
     1081 gaaggacagt taatgaaagg gaagatcaat tttgaatttg attatttcta taacaagcgg
     1141 aataatatct tatggcagaa acagggatcc acacccggtt cctccggtat tgcctattta
     1201 ctgcctccgg aaaacatcgg gaaaatacag aacacgggat atgaatttgt gttaggctat
     1261 aacggagatt tcgcgaacgg caagttcagg tattccgtga gcgtgaacgg cggatacgcg
     1321 aaaaacaaga tcctgttctt tgacgagaat cccggcgcac cggaatggca gaaagccacc
     1381 ggccacccct tcactgccaa tggaggcgcc gcctttcttg cctatcagta tgatggcgta
     1441 ttcgtagatc aggcggatat cgataagacc acgatcgatt acaccggcct gaccaatacc
     1501 ctccggcccg gcgacatgaa atttaaagac gtgaacggag acggcaagat caatgcggac
     1561 gaccaggaga gattaaataa aacgggagac ccgacattta ccgggggact gaatatccag
     1621 cttcagtaca gcaacttcga tctgtccatc cttttccagg gcgccaccgg aggcctgctc
     1681 tacatcggca cagaatccgg ggatatcggg aactacctgc aatactcata cgatcatcaa
     1741 tggaccatcg atcaccccag cagtgtggac ccgcgcatcg cgaacaggaa caatacctac
     1801 tatgccggtg gcgctgcggg taacaatacc tatttcctgc ggagcagcga ttatttccgc
     1861 ctgaagaatg ttgaactggg ttataatctt ccttccgagc tcggaaaaaa agtgggcatc
     1921 agtaacctga gattatatgt caatggcctg aacctggtaa cctgggacaa gatgaaaata
     1981 tgggatcccg aatccacccg cggagacgga caatattacc cgcaggccag aattatcaac
     2041 gtcggggcaa gagtaacttt ttaaaacgaa gaatatgaaa aaaatattta taaatataat
     2101 attgctcatc tccggggtcg tcctgatcag ttcctgtaag cgggattttt taaacacgga
     2161 gcccttaaat gcgatttcct ctgatgccac ctggaaagat gccggattgt cccaggcttt
     2221 tgtcacgaat atctataacg gcttgcagaa cggaggtttc tatgaacagg aactggcctc
     2281 cctctctgac gaagccgtat tcgtgcatcc gggacgcggc attaatgtta ttaatgaagg
     2341 aacgctgagc ccttccaatc tcggctggca ggatggcacg tatgaatggg ttaacatgta
     2401 caattacatc cggccctgca atctcagtct ggaaaacctg gcgactgccg gttttgatga
     2461 ccaggaatta aatgaccgcc tccgcgggga ggcgcatttt ctgcgcgcat attactacca
     2521 gcaactgctc cgttattacg gggccgtgcc catcgttgac catacctacg gcattaacga
     2581 agactacagc atcgcgagaa atacctacga agaatgtgtg aattttatcg tgaatgactg
     2641 tgacagtgcc gccatgttat tagacggcaa aacgctggac aagggtcgcg ccaccagtct
     2701 ttctgcaatg gcattgaaat cacgcgttct gttatatgca gccagtgacc tgcacgatat
     2761 tcctactgcc agcgcaaaat caagcgttat agccggctac gccaatccgg agttgctggg
     2821 atatgtgagc ggcgaccgcg ttgcccgatg gcaggctgca aaagatgctg ccaaagccgt
     2881 tctggatgcc agcagcggtt ataagctgga tctgactgca cccgcttctg ccgccgacgc
     2941 acaggcatac tatacttcga tcgccatggg cggtggaagc aatggccccg gtatagataa
     3001 agcggcttcc gttgaacttt tatttgaacg tgatttttcc tcccagctgg taagcggagg
     3061 ccccggcaca gacggcacat ccgtaggact ggacaatggg ccgaacggct atcataactg
     3121 ggcgggcaat gtgcccatcc aggaattggt ggatgattat gaaatgatgg acggttcaaa
     3181 attcgactgg agcaacggcg cagaaaaagc agccccttat aagaaccggg atccccgcct
     3241 ttatgctact attttgttcg atggtgcgga ctggaaaccc cgcgataaga tcaccagcaa
     3301 tcccgatccc gccaaccaga tccagagcgg ccagtaccag ggagccggcg gaacaaccat
     3361 tgccggtctg gatacacggc aaagcgttgt ggaaaactgg aatgccagct ggaccggcta
     3421 ttacatgaga aaattcattg atcccgatcc ggctgtcgtg gataacaata cacgtcaata
     3481 cataccctgg ccctttctcc ggtatacgga agccgtgttg aattatgcgg aagcctgtat
     3541 tgaactgggc caggaagcgg aagcagtgac ctggttaaac aagatccgtt tccgtgcggg
     3601 tatgcccgcg atcacggaat ccggatccgc gctcatgcag cgctaccaga atgaacgaag
     3661 agtagaactg gcctatgagg aacaccggta tcacgatgcc cgccgctgga tgatcgcgcc
     3721 aacaaccctg ggcaggccgg tcatagacat caatgtggtg ggcacgctga aacccggcaa
     3781 ggtggcgccc tcgccttatg tgcatgatga aagcatttat aactacagct atactccttt
     3841 ctcagataat ggtctcgaaa acaggatatg ggtggacaaa atgtatttca ggcccatcag
     3901 ccgggatgag gtaaacaaga acgcgaagct gattcaaaac cccggttacg atcaataagg
     3961 aataatcctt cctgatatta ctctgaaact atgccggtcg cccacgagac tggcatagtt
     4021 gttttatctt catcctttaa ttctgtaaaa attgaatcat tccagatctg gaccgttttg
     4081 tcttttactc ctcctttcaa tcatcgcttt ttccggatgt acaaataatc ccatagagaa
     4141 aaaaaatacg gccgcgcctt ccaataccct gttcacttta ctgcaaccgg agaaaacaca
     4201 tgtcagcttc agcaatacgt tgaccgaagg actcaatacg aatgtattga tctatgaata
     4261 cttctataac ggcggaggag tggcgctcgg ggatgtgaat ggagatggcc tgaccgatat
     4321 ctattttaca ggcaacatga ccgataacaa actctacctg aataaaggtc atatggaatt
     4381 tgaggatata accggctctg ccggcgtttc cggaaggccc ggcccctgga aaaccggggt
     4441 caccctggtt gatatcaacg gagacggccg cctggatatt tatgtttgtt attcgggcaa
     4501 actgagaggg gaaaaaagag tcaaccagct gttcattaac gacggcaacg acaatcaggg
     4561 aaggcctcat ttcagcgaac aggcagagca atacggcctg gccgattctt cctacagcac
     4621 gcaggctgtt ttttttgatt atgaccgcga cggagacctg gacatgctgc tgctcaatca
     4681 cagcattacc cggcgaacct cgcttgatga ggcgaccatt caaaaactgc tgcttgagaa
     4741 cgatcacctg agcggattga aattatttaa aaacgaccac gggcatttta cagaagtcac
     4801 tgccgccgcc ggtataaaga gcacggtcct atcccatgga ctgggcgccg ggatcgctga
     4861 cctgaatggg gatgggtggc cggatattta tatctccaat gattatcttg aaccggatta
     4921 tctgtatatc aataataaga acgggacgtt caccgatcag ctgcagaaaa cgcttggcca
     4981 cacttccttt ttttcgatgg gcaatgatat tgcggacatc aacaatgatg cattgcctga
     5041 tatttttacg ctggatatgc tgccggaaga caatcaccgg cagaaattat tattcgctcc
     5101 ggataattat gaaaaaatcg aggtcaacat tcattccgga ttctattacc agtatatgtg
     5161 caatatgctg cacctgaata acggcaacgg gagtttcagc gacattgcgc agctggcagg
     5221 catttcgaac acggactgga gctgggctcc cctgttcgca gactatgata atgacggatg
     5281 gaaggatctg tacgtgacca atggttacct gcgtgactat accaatatgg attttctgaa
     5341 atatatggga gatgcgctta aggacaggca ggtaatgaga aaggacctgc tggcgctcgt
     5401 acaggaaatg ccttcttccc gggtaaagaa ttatatgttc agaaataacg gcgaccttac
     5461 ctttcaaaac acaggcaaac aatggggatt tgacacggcc tccaatagta acggggccgc
     5521 ctatgcggac ctggacaatg atggcgacct ggatctggtc gtgaataata tcaatcagcc
     5581 cgcgttcatt tatgaaaatc aatcgaacag actgctcaac aatcattatc tggagctcag
     5641 gctggaggga tctggacaga atacccaggg cataggagct aaagtgacca tctaccttaa
     5701 gaataaacag cagtacctgg agcaaatgcc ggcccggggt tttcaatcca gtgtttcacc
     5761 cgttttgcac tttggactgg ggaaggaaag aactatcgat tctttaaaaa tcgtatggca
     5821 aagcggaaaa acggaaacgc tccgggatat taaaggagat caattgcttg cgctaaaaga
     5881 gaaagacgcc ctgataaagc cgcaaccggt taggatagca agtccggttt ttaaagaggt
     5941 aagatctccc atcagctttc atgcggtaac caatgagatc aatgatttca agaggcagcc
     6001 gctgctgatt aatcccttgt cgtttcaggg tccctgcctg gtgaaagggg atgtgaacgg
     6061 agatcatctg gaagatatct atgcgggtgg cggaagcggc caggccgggg ccttatatat
     6121 ccagcaaaaa aacgggcaat ttacccggaa agccgaaccg gcctttgaag ccgataaggg
     6181 gagcgaagat gccgacgccc tgttcatcga cgtaaacgga gacggatttc cggatctgta
     6241 cgtggccagc ggcgggtatc ataattattt accggatgac ctattattgc aggaccgcc
//
LOCUS       DASYCU010000656         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_431574, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000656 DASYCU010000000
VERSION     DASYCU010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431574"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VGM24_13210"
     CDS             <1..1007
                     /locus_tag="VGM24_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156391.1"
                     /translation="APYNVMPASIYNDSEYLSVPESRRESFRKQVLNGIPLGKGNYLR
                     LFPVWMDYRGHFGTILPQAQALASAALLRGDLASAELSMHQPEWITGENPFSQSTMYG
                     EGYDFTPLYSPSSGDMVGALPVGIQTRDEKDIPYWPVQSMWTYKEVWVHPVCRWIWLM
                     KDLAGPALIEGQADGKVEFKEITTGDSTLVEPDPLTSRFRISLPQGKYILRSNGKEQL
                     QTFLSTATYHPDLRSSHLLDFEISQAGNGTGKFKIRITARGNGKHVFRILTDNLITGN
                     AQKEVTLNPGMESTLEWPVSVSSAHIPWIAVIIPDGDMSRRKEIIESAPESRSSTKSF
                     "
     gene            complement(1020..1505)
                     /locus_tag="VGM24_13215"
     CDS             complement(1020..1505)
                     /locus_tag="VGM24_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY4156392.1"
                     /translation="MPEQKELFIKIALDAWNVYVDRAGKLFESLTDEQLREQVAPNRN
                     TAVYLLGHLIAVHDGILPLLGFRDKLYPSLEEVFIKNPDRSGLEKPPIPVLRQYWKDV
                     HNILAQHFDKLNTEEWLQKHNSISAEDFAREPHRNRLNVLVNRTNHLASHYGQLIFLK
                     K"
     gene            complement(1714..2826)
                     /gene="pstS"
                     /locus_tag="VGM24_13220"
     CDS             complement(1714..2826)
                     /gene="pstS"
                     /locus_tag="VGM24_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY4156393.1"
                     /translation="MEILSRVKGLFHLLVVACGFGFLMTGCNGTGGGEQGGDSTQAKA
                     NSGNTLLGAGSTFVYPLFSKMFSEYHDATGIKVNYQSIGSGGGILQITNKTVDFGGTD
                     APLNEEQTQKAGAEMLHIPVASGAAVVSYNLPGFQDTLKLTPQLLADIFLGKIKTWTD
                     PGITSANPGAKIPSLPIIVVHRSDGSGTTFIWTDYLSKVSEEWKAKVGKGTAVNWPAG
                     LGGKGNEGVSGLVKQTPGGIGYMELAYAVQNKMPAAAVKNKAGHFIYPDLSSITAAGN
                     IQLPADAKVSITNTDAADGYPVSSFTWAIIYKDQKYGDRNMDRAEKLVKLLWWAIHEG
                     QAYCKQLNYAPLSLSALQVGESILKSATYGGKPVLP"
     gene            complement(2929..>3466)
                     /locus_tag="VGM24_13225"
     CDS             complement(2929..>3466)
                     /locus_tag="VGM24_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY4156394.1"
                     /translation="QRDTLTGYYKEGIRRVWEKARQNAFYRGIPFIWCSNNFTVAFAI
                     QCLWYHQLSGDPEFDELEQANIDWLLGCNPWGTSMIYGLPAHGDTPDQPHSSLNHLAG
                     YPIDGGLVDGPVYGNIFNNLIGITLSHPDEYAAFQSQLAVYHDDAGDYSTNEPTMDGT
                     ASLIYLLASKENESRARR"
BASE COUNT          881 a          881 c          808 g          896 t
ORIGIN      
        1 cagcacccta taacgtaatg cccgcatcta tttacaatga cagtgaatac ctgagtgtac
       61 cggaaagccg ccgggaatca ttccgcaaac aggtactcaa cggaattccc ctgggaaaag
      121 gaaattacct gcgcctgttt cccgtatgga tggattatcg cgggcatttt ggaacgatac
      181 ttcctcaggc ccaggcatta gccagcgccg ctcttttacg cggagacctg gcatcagcag
      241 aactttccat gcatcagccc gagtggataa ccggagaaaa tcctttttcg caaagtacca
      301 tgtacggaga aggctacgat tttactcccc tctactcacc ttcttccggg gatatggtcg
      361 gcgctttgcc ggtaggtatt cagacccggg atgaaaaaga tattccctac tggccggtgc
      421 agagtatgtg gacttacaaa gaagtatggg tgcatccggt ttgccggtgg atctggctga
      481 tgaaagatct tgccggtccg gctcttatag aaggacaggc cgatgggaaa gttgaattta
      541 aagagatcac tacaggagat tcaaccctgg tggaaccgga tccgctaacc agccgtttcc
      601 ggatcagcct gccgcaaggg aaatacattc tgcgttcaaa tggaaaagaa caattgcaga
      661 ccttcttatc caccgctacg tatcatcccg acctgcgctc ctcacacctg cttgattttg
      721 aaatttcaca agccggaaac ggcacgggga aatttaagat ccggattact gcgcggggaa
      781 acgggaagca tgtgttccgc atcctgacgg acaatctcat aactggcaac gcgcagaaag
      841 aagtaacgct taatccgggt atggaatcca cgctggagtg gcccgtcagc gtttcgtctg
      901 cgcatatccc ctggatcgca gtaatcatac cggatgggga tatgagccgg cgaaaggaaa
      961 taatcgaatc tgccccggag tcccggagct caaccaaatc tttttaattc aacgggttgt
     1021 tattttttta aaaaaatcag ctgaccgtag tgtgaagcca ggtggtttgt ccggttgacc
     1081 aaaacattta accggttgcg gtggggttcc ctggcaaaat cttcggcaga aatggaattg
     1141 tgcttttgca gccattcttc cgtattcaat ttatcgaagt gttgtgccag gatattgtgc
     1201 acatccttcc aatactgtct gagcacgggt ataggcggtt tctcaagtcc cgacctatcc
     1261 ggatttttta taaatacttc ctccagtgaa gggtatagtt tgtcccggaa accaagcaat
     1321 ggcaatattc catcatgaac agcaataaga tgccccaata aatatacggc cgtattcctg
     1381 tttggcgcaa cctgctccct gagttgttcg tctgtaaggg attcaaacaa tttacctgcc
     1441 cggtctacat aaacattcca ggcatccaaa gcgatcttaa taaataattc cttttgttcc
     1501 ggcattttct ttttttaatt ggttattaga ttatcccggg gagctcacta ccgtttctcc
     1561 ggtgtaatcc atccttaata ttaacccttt tcagaaaatt atgaaaaaat atcccgacca
     1621 taatttggcg ggtggggtaa aagcaaaaat aagggctttg gcgtttaacc aaacccctaa
     1681 gtaatttcgt tattcgctcc ggcagcaggc ggtctaaggc aaaaccggct tgccgccgta
     1741 agttgccgac ttcagaatgg attctcctac ctgcaaagcg gacagggaca ggggtgcata
     1801 gttcagctgt ttgcaatagg cctgtccttc atggatcgcc caccataaga gttttaccag
     1861 tttttccgcc cggtccatat tgcggtcgcc gtatttctga tctttataaa taatcgccca
     1921 ggtaaaactg ctgaccggat acccatccgc ggcatccgta ttggtaatgg acactttagc
     1981 gtctgccggc agctgaatat ttccggcggc cgtaatggaa gaaagatcag gataaataaa
     2041 atgaccggcc ttatttttga ctgcagcagc cggcattttg ttttgcactg cataagccag
     2101 ttccatatac ccgatacctc cgggagtttg tttaaccagg ccggaaacgc cttcattgcc
     2161 tttccctccc aacccggcgg gccagttgac ggccgtgccc ttgcccactt tggccttcca
     2221 ttcctcactc accttggaaa gataatccgt ccagataaag gttgttccgc ttccatcgga
     2281 ccggtgcacc acaataatcg gcaatgacgg tattttagca cccggatttg cagaagtgat
     2341 tcccggatcg gtccatgttt ttatttttcc cagaaaaata tcagcaagca gttgcggcgt
     2401 cagtttcagg gtgtcctgga aacccggcag attatagctg accacggcag caccggaagc
     2461 taccggaata tgcagcatct cagcgccggc cttttgggtt tgctcctcat tcaatggagc
     2521 atccgtaccc ccgaaatcaa ccgttttatt ggttatctga aggattccgc cgcccgatcc
     2581 gatggattga taatttactt tgattccggt ggcatcatgg tactcgctga acatctttga
     2641 aaagagcgga taaacaaagg tgcttcccgc ccccagtaat gtattgccgg aatttgcttt
     2701 cgcctgtgtg ctgtcgcctc cctgctcccc tccgcccgtt ccattacagc cggtcatcaa
     2761 aaaaccgaaa ccacacgcca ctacaagaag atggaataac ccttttaccc gggataatat
     2821 ttccatggaa attacaatta aggatgaata agttgccagg acctcataaa ggtatagccg
     2881 atccgctctt taataagcgg cccaatgtaa aggaattatg aactttaact agcggcgcgc
     2941 ccggctctcg ttttcttttg aggccagcag ataaatcagg gatgccgtgc catccatggt
     3001 aggttcgttg gtgctgtaat ctccggcatc atcatgataa acggcgagct ggctttggaa
     3061 tgccgcatat tcatccggat gggataaggt gattccgatc aggttattaa aaatattacc
     3121 gtaaaccgga ccatcaacca gcccgccgtc gatcggatac ccggccagat gatttaatga
     3181 ggaatgcggc tggtcaggcg tatctccatg cgccggcaaa ccatagatca tggaggttcc
     3241 ccatggattg caacccaaaa gccagtcgat atttgcctgt tccagttcat caaattccgg
     3301 gtccccgctc agttgatgat accacaaaca ttgaatggca aaggcgaccg tgaaattatt
     3361 actgcaccag ataaatggga tgccgcggta aaaagcattt tgtctggctt tttcccatac
     3421 gcgccggatc ccttccttat aatatccggt cagggtgtcc cgctga
//
LOCUS       DASYCU010000657         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1156053, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000657 DASYCU010000000
VERSION     DASYCU010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156053"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..562
                     /locus_tag="VGM24_13230"
     CDS             155..562
                     /locus_tag="VGM24_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156395.1"
                     /translation="MKKEEIPQDLGALGKITQEVCYAVDEAGKYSTALSNGWEVKSSA
                     LDVAWKDVQQRIQTARQRVLDKEASPLLFFMERHVMDIPIVASYTGFWKWQVKRHLKY
                     AVFEKLSEARLEKYATLFEVSVADLKTMKTNET"
     gene            552..1568
                     /locus_tag="VGM24_13235"
     CDS             552..1568
                     /locus_tag="VGM24_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HEY4156396.1"
                     /translation="MKLDFQHTQAAHCENGVTTSLLRHHGVNQITEPLAFGIGSGLFF
                     IYIPFMKVNNGPAIAFRTQPGLIFKRTCRALDIPVERKKFRSKEEAERVLMECLKAGQ
                     PAGCQVGVYYLTYFPKEYRFHFNAHNLIVYGKEDDHYLISDPVMEMATSLSSYELERV
                     RFAKGAFAPRGQLYYPKEKRTVTDEQMKKAIRSGIKHNIRDMLHIPGNIAGIRGIRYT
                     AKKIRGWRDKLGLEKAGSYLAQLVRMQEEIGTGGGGFRFIYGAFLQEAFAYHPLDELL
                     EISRQFTRSGDVWRSAAVQAAGIYKGRIGSQADFNTMSDYLNQAADIEKQAFVALSKI
                     KWPA"
     gene            1565..2311
                     /locus_tag="VGM24_13240"
     CDS             1565..2311
                     /locus_tag="VGM24_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY4156397.1"
                     /translation="MSIPAIAIRSLEFSYPGQENMLFEGLSLEVADGERFGLFGPNAA
                     GKTTLMNLMTGLLRYKHGSIRLLGNEMNRHAKPVNRLFGFVPQDFSFYAELSPRENLS
                     FFGAWYGLSAAQIKKRTSELLEVLGLADVGNRLLHTFSGGMKRRVNLAIGVMHQPRVL
                     FLDEPTVGVDVQSRHAIIHYLKELNRQGTTLIYTSHQLEEAEGLCERIAMMDNGRIIA
                     HDQLDNLLEEHRQEGLEGLFLGLTGKRFRD"
     gene            2314..>3010
                     /locus_tag="VGM24_13245"
     CDS             2314..>3010
                     /locus_tag="VGM24_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY4156398.1"
                     /translation="MQTLTATIIKDLKILMRDRLGLLMMFFMPVLLVIIITSVQNSTF
                     ELVNNNKVTMLACNRDKGPEGARLMEAMEKIGLFKLLPVDPSVTANEISTRMYARDAL
                     VALVIPEDFSSSIQNKATDIASRALRDSESPEEAIKQNTDQPDSLIMFYHPVMQQSFR
                     GSIEGALHSAVQVIQGEAIVKNLYSAINSKTIPKDLEKEILFGQIPITEFPVTRSGSR
                     VIPNASQHNVPAWT"
BASE COUNT          772 a          737 c          820 g          681 t
ORIGIN      
        1 atcggtctat tttatggtgg atgaagtttt aaagaccagg gacctgaaaa agggtcagaa
       61 aatattgttg ctggttccgg aaagctcccg gttctcctat atgtatgcgc tgctgacggt
      121 gtgctgacgg gtgatcggcc aaaaaatact ctgcatgaaa aaagaagaaa tcccgcagga
      181 tctcggtgcg ttggggaaaa tcacccagga ggtctgttat gcggtggacg aagccggaaa
      241 atacagcacg gcgctcagca atggctggga agtaaagtcc tctgcgcttg atgttgcctg
      301 gaaagacgtg caacagcgca ttcagacggc ccgccagcgg gtgctcgata aagaggccag
      361 tcccctgctg ttttttatgg aacggcatgt catggacatc cccatcgtag cctcctatac
      421 cggtttctgg aaatggcagg tgaaaaggca tttgaaatat gccgttttcg aaaaactttc
      481 agaagccagg ctggaaaaat atgcgaccct ttttgaggtg agcgtggccg atcttaaaac
      541 gatgaaaaca aatgaaactt gattttcagc atacccaggc cgctcattgt gagaatggtg
      601 tcaccaccag tctgcttcgc catcatgggg taaaccagat cacggagcct ttggcgttcg
      661 gtatcgggtc cgggctgttt tttatttata ttccattcat gaaggtgaat aacgggccgg
      721 ccatcgcctt ccggacccag cccgggctga tttttaaaag gacctgcagg gcactggata
      781 ttcccgtcga aagaaaaaaa ttcagatcga aggaagaagc cgaaagggtc ttaatggaat
      841 gcctgaaagc aggccagccc gccggttgtc aggtgggcgt atattacctc acttactttc
      901 cgaaggaata ccgttttcat ttcaatgccc ataacctgat cgtttacggc aaagaagatg
      961 accattacct gatcagtgat ccggtgatgg aaatggccac ctccttgagc agttatgagc
     1021 tggaaagagt gcgctttgcc aaaggggctt ttgcgccgcg cgggcaactg tattatccaa
     1081 aggaaaaacg caccgtaacg gatgagcaaa tgaaaaaagc catccggtcg ggaataaaac
     1141 ataatatccg cgatatgctg catatcccgg gaaatattgc cggaatccgg ggcatccgtt
     1201 ataccgcaaa aaaaatccgc ggctggcgcg acaagcttgg cctggagaaa gcgggaagtt
     1261 acctggccca gctggtcagg atgcaggaag agatcggcac gggaggaggc ggattccgtt
     1321 ttatctatgg cgctttttta caggaagcat ttgcctacca tccgctggat gagttgcttg
     1381 aaatttcaag gcagttcacg cggtcaggag atgtgtggcg ttccgctgcc gtgcaggcag
     1441 ccggcatata taagggccgt atcggaagcc aggccgattt caataccatg agcgactacc
     1501 tgaaccaggc ggccgatatt gagaagcagg catttgttgc cctgtcaaaa ataaagtggc
     1561 cggcatgagc attcccgcca tagcgatccg gtcgcttgaa ttcagttatc ccggccagga
     1621 aaatatgctg tttgaggggc tcagcctgga agtggccgac ggcgaacgtt tcggattatt
     1681 cggccccaat gctgccggaa aaacaacgtt gatgaacctc atgacggggc tgctccggta
     1741 taaacacggg agcatccgcc tgctgggaaa tgagatgaac cgccatgcga aaccggtgaa
     1801 tagattgttc ggattcgttc cgcaggattt ttccttttat gccgaactga gtccccggga
     1861 aaacctttcc ttttttggcg cctggtatgg attaagtgcc gcacaaatca agaagagaac
     1921 gtccgaatta ctggaagtgt tgggtctggc ggatgtcggc aaccgcctgt tacatacttt
     1981 ttctggcggc atgaaacgcc gggtaaacct ggccatcggc gtaatgcatc agccccgggt
     2041 ccttttcctg gatgaaccga cggtgggagt ggatgtgcag tcccgccatg ccattattca
     2101 ttatctcaaa gaactgaacc ggcagggaac cacgctgatc tatacttccc atcaactgga
     2161 ggaggcggag ggtttgtgcg agcggattgc catgatggac aacggcagga tcattgccca
     2221 tgaccagctc gacaatctgc tggaagaaca ccggcaggaa ggactggagg gtttattcct
     2281 tgggctcacc ggtaaacgat tcagggactg accatgcaaa cactaaccgc caccattatt
     2341 aaggatctga aaatactcat gcgggaccgt ctgggcctgc tcatgatgtt tttcatgccg
     2401 gtattactgg tgatcattat taccagtgtg cagaacagca cgtttgagct ggtcaataac
     2461 aacaaggtta cgatgctggc ctgtaaccgc gacaaagggc cggagggagc ccgtctgatg
     2521 gaagccatgg aaaagatcgg gctgtttaaa ctgctgccgg tcgatccttc cgtcacagcc
     2581 aatgaaatct ccacccgcat gtatgcccgg gacgccctgg tggctttggt gataccggag
     2641 gatttttctt cttccattca aaacaaggca acggatatcg cgtcccgcgc cctgcgggat
     2701 tcggagtccc ccgaagaggc aataaaacaa aatacggatc aaccggattc cctgatcatg
     2761 ttttatcatc cggtaatgca gcaatccttt cgcggctcta ttgaaggcgc attgcacagc
     2821 gccgtacagg tgatccaggg agaggccatc gtgaaaaacc tgtacagcgc cattaacagc
     2881 aagaccattc caaaagatct ggagaaggag atcctcttcg ggcagatccc catcaccgaa
     2941 ttcccggtga cccgatccgg cagccgggtc attcccaatg cttcccagca taatgtaccg
     3001 gcctggacca
//
LOCUS       DASYCU010000658         6333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3041912, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000658 DASYCU010000000
VERSION     DASYCU010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3041912"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /gene="ftsH"
                     /locus_tag="VGM24_13250"
     CDS             complement(<1..1822)
                     /gene="ftsH"
                     /locus_tag="VGM24_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY4156399.1"
                     /translation="MKQNERPGFPRFRPKNGDDNNPKKGPRFSIYWVWGIIALILFSY
                     SYFGAFSPDARKLDSEMEFRNNMLDKGDVSRLVLVTNKNLVRVYIKKDSLDKPYYKDK
                     LGKSYQVVKTEGPQFEFQVTKIDDFEKRMSDIFAKNPSMEVPMSPIQEGDWIPGLLQI
                     ALPILIILLIWVMLMRKMGGQGGSGGPGGIFNIGKSKATLFDKGTKVNITFNDVAGLD
                     EAKVEVMEIVDFLKNPKKYTALGGKIPKGALLVGPPGTGKTLLAKAMAGEAQVPFFSM
                     SGSDFVELFVGVGASRVRDLFKQAREKAPCIIFIDEIDAIGRARGKNAIMSNDEREST
                     LNQLLVEMDGFGGDSGIIILAATNRPDVLDSALLRPGRFDRQISIDKPDLKGREAIFK
                     VHLKPIKISSKLDIYKLAEQTPGFAGADIANVCNEAALIAARKGKDSVEMEDFQDAVD
                     RVIGGLEKKNKIISPEEKRIIAYHEAGHAICGWYLEHAYPLLKVTIVPRGVAALGYAQ
                     YTPKEQYLYNTDQLFDQICMTLGGRASESLNFNKISTGAQNDLQQVTRIAYSMVTVYG
                     MNEKIGNVSFYDPAQENQFTKPYSEETSKLIDEEVRILIDK"
     gene            complement(1922..2326)
                     /gene="rsfS"
                     /locus_tag="VGM24_13255"
     CDS             complement(1922..2326)
                     /gene="rsfS"
                     /locus_tag="VGM24_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY4156400.1"
                     /translation="MEQLSMLVSRKPNPLARLAKNAKILKTIIQAIQEKKGEKIISLD
                     LRKIHEAVADFFIVCEAGSTTQVRAIADFVETEVKHKCGELPYKHEGKQVLQWVIIDY
                     INVVVHIMLPETRRFYKLEEMWSDAVMDEHEG"
     gene            2433..3194
                     /locus_tag="VGM24_13260"
     CDS             2433..3194
                     /locus_tag="VGM24_13260"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY4156401.1"
                     /translation="MDKLPGTVFDNQLIELDSVDSTNNYAMARIHAGLAFPGQVYFAH
                     RQWAGKGQRGKNWISEPGQNISMSLVLDPRSLPLSGQFLLGAAVSLACLKKVQEYVPE
                     GWSLKWPNDLYWRDRKAAGILIENIIKGKEWLYAVIGVGMNLNQTSFPPELSHAVSLK
                     QISSQSFEPLVFARELAARIGEHIAVLQQDAAGILKSFNQSLYKRGETVQLKKGNTRF
                     ETRIRSVDAQGRLLTVDSAERKFSHGEVEFILDGE"
     gene            complement(3210..4910)
                     /locus_tag="VGM24_13265"
     CDS             complement(3210..4910)
                     /locus_tag="VGM24_13265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY4156402.1"
                     /translation="MGKLSICLILVLLCGRLHAQLFQPVNYPKTEFRNPLSIPMSLAA
                     NFGALRSNHYHMGLDIRTRQRVNLPVYAAADGYIWKIKIEPFGFGQAVYIRHYNGYIT
                     LYAHLNAFYPALAAYIRKKQYELERWNVFLDLPSGLFPVKKGDLIAYSGSTGGSEGPH
                     LHFEIREYPADINLNPMLFGLPVPDNTAPVVRKLAVYDRNKSIYEQSPAVYAVNRSGG
                     LYRPVPPLIYVHSTQVGFGISGFDTQTGSSNPNGIYQAILYDGQQPVSGFRMNEISYN
                     DTRGINAHIDYRTKARGGPYYQLLFQLPGYVHSIYQQKAGGRIPLGDGKIHAIRIELK
                     DPYGNSSDLRFNVQYKPGIPSNTVFEGKTFYPFMVNGFEAEDCAFYLGERSLYDSVHV
                     SYSATDGNTAQAVSRLHHIGTATVPIQDSMTVRIKMTRELGDKNQVLMQRFDRGNSEV
                     RKVQWLGDWATAAFRNFGNFQLVLDTVPPGISFPGTAENANLQRSPGITVLAHDNFKQ
                     IENFRATLDGKWLLFTNDKAKAFIYHFDEHCPPGKHELKVSVEDEAGNGSQKVLHFIR
                     "
     gene            5101..5316
                     /locus_tag="VGM24_13270"
     CDS             5101..5316
                     /locus_tag="VGM24_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156403.1"
                     /translation="MTRSSNKMAVFFAISVGSLIGMVRGEEAKQKWVFKIRSKYDNKR
                     IIAEKRQRAREGGVLLDDIEMASFHDN"
     gene            5436..>6333
                     /locus_tag="VGM24_13275"
     CDS             5436..>6333
                     /locus_tag="VGM24_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY4156404.1"
                     /translation="MPKNSSIGSVLIIGSGPIIIGQACEFDYAGSQAARSLREEGIKV
                     ILINSNPATIMTDPMMADRVYLLPLTVESIEQILEENQIDAVLPTMGGQTALNLCKEA
                     AELGVWEKYSVQLIGVDIDAIDTAEDREKFRQLMVKIGIAVAPSRVANSLLEGKEFAQ
                     DIGFPLVIRPSFTLGGTGGSFVLNKDELDGALDKGLKASPIHEVLVEKAVLGWKEFEL
                     ELLRDKNDNVTIICTVENMDPMGVHTGDSITVAPAMTLSDTAFQDMRNKAILMMRSLG
                     NFAGGCNVQFALNPETEEIISVE"
BASE COUNT         1571 a         1539 c         1541 g         1682 t
ORIGIN      
        1 ctttatcgat cagtatccgg acctcttcat caatcaattt ggacgtctcc tccgagtaag
       61 gtttggtgaa ctgattttcc tgggccggat cataaaagct cacgttcccg attttctcgt
      121 tcattccgta aaccgttacc atggaataag caatccgggt aacctgttgc agatcgttct
      181 gggccccggt ggagatctta ttaaaattca gggactcact tgcccttccg ccaagggtca
      241 tgcagatctg atcgaacaac tgatccgtgt tatagagata ttgctctttg ggtgtatact
      301 gggcataacc gagcgcagcc acgccgcgcg gaacgatggt caccttcagg agcggatagg
      361 catgttccag gtaccagccg caaatggcat gaccggcttc gtgataggca atgatccgtt
      421 tttcctccgg ggaaatgatc ttgttctttt tttccagccc gccgatgacc cggtccaccg
      481 catcctggaa atcttccatc tccacgctgt cttttccttt tctggccgct attaatgcgg
      541 cttcattgca aacattggcg atatccgcgc cggcaaatcc gggggtttgc tcggccagtt
      601 tataaatatc gagcttggag gaaattttta tcggcttgag atgcaccttg aagatcgctt
      661 cccttccctt cagatccggt ttgtcgatgg agatctgccg gtcgaacctt cccggccgaa
      721 gcagcgctga atccagtaca tccggtcggt tggtggcagc cagtatgatg atcccgctgt
      781 ctccgccgaa accgtccatt tccaccagca gctggtttaa ggtgctttca cgttcgtcat
      841 tgctcatgat cgcattctta ccgcgggccc ggccgatagc atcaatttca tcaatgaaaa
      901 tgatacaggg cgccttttcg cgggcttgtt tgaacaggtc gcgaacccgg cttgcgccca
      961 cacccacaaa gagttccacg aaatctgaac cggacatgga aaaaaacgga acctgcgctt
     1021 cacccgccat cgcttttgcg agcagggttt tacccgtgcc cggaggacca accagcaaag
     1081 cccctttggg gatctttccg ccgagggccg tatatttttt aggatttttc aggaagtcga
     1141 cgatttccat cacctccacc ttcgcttcat ccagacccgc aacgtcgttg aatgtgatat
     1201 tcaccttcgt tcccttatca aataaggtcg cttttgattt cccgatatta aagatcccgc
     1261 ccggaccgcc actccctccc tgtccaccca ttttgcgcat cagcatcacc cagatcagca
     1321 gaatgatgag gattggcagg gcaatctgca ataaacccgg gatccagtct ccctcctgta
     1381 tgggagacat gggcacttcc atagaaggat tcttggcgaa aatatcgctc atcctttttt
     1441 caaaatcgtc gatcttggta acctggaatt caaattgggg cccttcggtt ttcaccacct
     1501 ggtaactctt tccgagcttg tccttgtaat agggtttgtc cagactgtcc ttctttatgt
     1561 aaaccctgac caggttttta ttggtgacca gcaccagccg ggaaacatcc cccttgtcca
     1621 gcatattatt tctgaattcc atttcagaat ccagcttgcg ggcatccggt gaaaaagccc
     1681 cgaaataact atagctgaag agaatcaggg cgatgatgcc ccatacccag taaatactga
     1741 accggggacc ctttttgggg ttattgtcat ctccattctt cggtctgaac cggggaaatc
     1801 caggcctttc attttgtttc atattatttt gtggcatgtg tggtcttcct tgtcttagta
     1861 attaatgttt ggggaataaa aaagttcgga aaatgcgttt aaaacatagc tagatcgttg
     1921 attatccttc atgctcgtcc ataaccgcat cgctccacat ttcttccagt ttgtagaagc
     1981 ggcgggtttc aggaagcata atgtggacaa ccacgttaat ataatcaatg atcacccatt
     2041 gtaatacctg ttttccttcg tgcttatagg gaagttctcc gcatttatgt tttacctcag
     2101 tttcaacgaa atccgctatg gctctgactt gtgttgtgct gcctgcctca cagacaataa
     2161 agaagtctgc taccgcttca tgaattttcc ggagatcaag agatattatt ttttcgcctt
     2221 ttttttcctg gatggcctga ataatggttt ttaagatttt agcgttcttc gctaaccggg
     2281 ctaacggatt tggtttccgg ctaaccaaca tagacaattg ttccaataat cagaatattt
     2341 tgatgataaa tagtgttaag cgaagtatca gaaactgtag gaatactaat ttcgcaatgc
     2401 ccaaaaatag taatcttttg ccacatccca ctatggataa actacccggg accgtatttg
     2461 acaatcagtt gattgagctg gattcggtgg acagcaccaa caactatgcc atggcccgga
     2521 tccacgccgg gctggctttt ccgggccagg tttattttgc tcaccgtcag tgggccggca
     2581 aaggacagcg cggaaagaac tggatcagcg agcccggtca gaatatcagc atgagcctgg
     2641 tgctggaccc ccggtccttg cccctgtccg gccaattcct tctcggggca gccgtgtccc
     2701 tggcctgcct gaagaaagtc caggaatatg ttccggaagg atggagtttg aaatggccaa
     2761 atgatttgta ctggcgtgac agaaaggcag cagggattct gatcgaaaat ataataaagg
     2821 gtaaggaatg gctttatgcc gtgatcggcg tgggaatgaa tctgaaccag accagctttc
     2881 caccggagct ttcccatgcc gtttccctaa aacaaatcag cagtcagtct ttcgaacccc
     2941 tggtattcgc gcgcgaactg gccgcccgga tcggggagca tatcgctgtt ttgcagcagg
     3001 acgccgcagg cattctgaaa tctttcaatc aatccctgta taaacggggt gaaaccgtac
     3061 aacttaaaaa gggaaacacc cggtttgaaa cccgtatccg gtcggtggat gcgcagggca
     3121 ggttgctgac ggtggattct gccgaacgga aattttcaca tggcgaggtg gagtttattt
     3181 tggatgggga atgaccactc agccatcgcc tagcggataa aatgcaaaac cttttgcgac
     3241 ccgtttccgg cctcatcttc cacactgacc tttaattcgt gtttgcccgg cgggcagtgt
     3301 tcatcgaaat gataaatgaa cgcttttgcc ttatcgttgg taaataaaag ccatttgccg
     3361 tccagggttg cccggaagtt ttctatctgt ttgaaattat cgtgcgcgag tacggtgatt
     3421 cccgggctcc tttgcaggtt tgcattttcg gctgttccgg gaaaagagat cccgggcggt
     3481 acggtatcga ggaccagttg aaagttcccg aaattccgga aagctgccgt agcccagtca
     3541 cccagccatt gcacttttct gacttcagaa tttccccggt cgaaccgttg catcagcacc
     3601 tggtttttat cgcccaactc cctggtcatc tttattctta ccgtcatgga atcctgaata
     3661 ggaacggttg ccgttccgat atgatgaagg cgtgaaacgg cctgggctgt attcccatcg
     3721 gttgccgaat aggaaacatg aacagaatca tacagggatc tttctcccag ataaaaagca
     3781 caatcttccg cttcaaatcc gtttaccatg aaggggtaaa aagtcttccc ttcgaaaacg
     3841 gtatttgacg ggataccggg tttatactgc acattaaatc ttaaatcgga gctgtttccg
     3901 tacggatctt tgagctcgat ccggatagca tgtattttcc cgtccccaag cggtattcgg
     3961 cctcctgctt tttgctggta aatggagtgc acatagcccg gcaattgaaa taaaagctga
     4021 taataaggtc cgcccctggc tttcgtccgg taatcaatat gcgcattaat gccgcgggta
     4081 tcattgtaac tgatttcgtt catcctgaaa cccgagaccg gttgctggcc gtcataaagg
     4141 atcgcctggt agatcccgtt cggattggac gatccggttt gggtatcgaa tcccgaaata
     4201 ccaaaaccca cctgggtgga atggacatag atcagcggcg ggacgggccg gtataggcca
     4261 ccggaccggt tcacggcgta aacagcagga gattgttcat atatactttt gttccggtcg
     4321 taaacggcca gctttcgaac caccggtgcg gtgttgtccg gaaccggtaa tccgaaaagc
     4381 attggattca ggtttatgtc cgcgggatat tcgcggatct cgaaatgcag gtggggaccc
     4441 tcggagccac ctgtacttcc gctgtaggca ataaggtctc ccttctttac gggaaatagt
     4501 ccggaaggca gatccagaaa aacattccac cgttccagct cgtattgttt tttcctgata
     4561 taggctgcca gcgcgggata gaaagcattc agatgagcat acagggtaat atatccgttg
     4621 tagtgcctga tgtaaactgc ctgtccaaaa ccaaagggtt caatttttat cttccatata
     4681 tacccgtcgg cagcggcata cacgggcagg ttaacccgtt gccgggttcg gatatcaagc
     4741 cccatatggt agtgattgct tcggagcgcg ccgaaattgg ccgccaggct catcggaatg
     4801 gaaagtggat ttctaaattc cgttttcggg taattaaccg gttgaaaaag ctgggcatga
     4861 agccggccgc aaagcaaaac caggatcaga cagatactca atttccccat gaattatccc
     4921 atttttagaa tggcgaaggt ccttaatccc aaccgaggat tccaaggcaa tcggtaaata
     4981 gatcataaat atttaatacc gcctgaaaat caatgatttt aaaaaaatgt caaaattggt
     5041 acagcttttg gcataaataa gctgtctata tattgacaag aacattatta aaaggaggtt
     5101 atgaccaggt cctcaaataa aatggctgtt ttttttgcta tcagcgttgg ctcgctgatc
     5161 ggaatggttc gcggcgaaga agcgaaacaa aaatgggtct ttaagatcag atcaaaatac
     5221 gataataaga gaataattgc cgaaaaacgc cagcgtgcca gggaaggggg cgttttactg
     5281 gatgatattg aaatggcctc ttttcacgat aattgatatt ccccgaagat ttttatatgg
     5341 tcccgctgat gcgggacttt ttttatttcc cccgatttac cgataaattt ctccgggaac
     5401 ccgtacaacc ttacttttgc ggaatttgtt ttgacatgcc aaaaaactcc tcgatcggtt
     5461 cagtactcat tatcggctcc ggccccatta ttatcggaca ggcttgcgaa tttgattatg
     5521 caggctccca ggccgccaga agcctccggg aagaaggaat aaaagtgatc ctgattaaca
     5581 gcaatccggc gacgatcatg acggatccca tgatggcaga ccgggtctac ctccttccgt
     5641 taaccgtgga aagcatcgag cagatcctgg aagagaacca gattgatgcg gtgttgccaa
     5701 ccatgggcgg acaaaccgcc ctgaatttat gtaaggaggc tgccgagctg ggcgtttggg
     5761 aaaaatatag cgttcaattg atcggcgtcg atatagatgc cattgatacg gcggaggacc
     5821 gggaaaaatt ccggcaactg atggtaaaga tcggtattgc cgtggctcct tcaagggttg
     5881 ccaattccct cctggaagga aaagaatttg cccaggacat cggcttcccc ctggtgatcc
     5941 ggccctcctt taccctgggt ggcacgggcg gtagtttcgt tttgaataag gatgaactgg
     6001 atggagcgct ggacaaaggg ctgaaagcat ctccgattca tgaagtgctg gtggaaaaag
     6061 ccgttctggg ctggaaagag ttcgaactgg agttattgcg ggataagaat gacaatgtaa
     6121 ccattatctg caccgtggag aatatggacc cgatgggcgt tcataccggg gattcgataa
     6181 cggttgcacc ggccatgacg ctaagcgata ccgcattcca ggatatgcgg aataaggcca
     6241 tcctgatgat gcgttcgctc ggaaactttg ccggcggatg taatgttcag tttgcattga
     6301 acccggaaac ggaagaaatt atttctgtgg aga
//
LOCUS       DASYCU010000659         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_3478799, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000659 DASYCU010000000
VERSION     DASYCU010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3478799"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2283)
                     /locus_tag="VGM24_13280"
     CDS             complement(<1..2283)
                     /locus_tag="VGM24_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156405.1"
                     /translation="MRKRLFAAVKLFLFCSFLNAGYTSAQPLSGFNYNGSFHPLENSI
                     RNKVQFNGYTTYWHDDYRQWYRYGNLFKMTAPDPSLTIMQSKRDIAEELGLPGLQMQE
                     GFLEGLLSEEFSAAEEPSLSELEAKIRTGNVLVFTSPESETGKKLMGKFSPDSSWRRE
                     LKSHQYGAVDFREVNAFYLDNGSRKIFVVCAIDSGMRRKVEELIGNTSRILREYDLHK
                     GWFGAETLLKSVTCEPGHPLEIIGKGMNEGNDWFTFGGYMEFLEKDELASWIAKVDLP
                     VVADVGFSPVYGCKDYNGLQVQDMNGREPWVKFAHEKGGYVFRRVYDTAADAYHYDGY
                     IATEGNKEQIDNENIPFISTTGSLQDGLVSSMVLFIKKGEKLTKPGMWDAIMHRREVA
                     ILEEGKMMGPAEYRHALQMLLLDRVFLEEYFGDRIDLQAEMDNYTLRISLSNTYDHPV
                     SGSLEIKLPPELKLAAAIPTELALPANSTKNIEIRILPMAEAMDKTNPVAVRFQWGKK
                     TKATLAILDLPPAISVHRLLYGLAPKIKYPVTIHNFSTANSYPVEVQVYEKNNPAKPV
                     FTSRQNGTAASGSFQNMLFELALPQGNYTVKVSALGLDYSSQLGVGKPEGQTQAYTID
                     LNHDGVSEYRMENDSVLVTLLATGGRVIEYIVKSRKDNIFFKLWPEKADDDKRPFRQG
                     REYYPYGGFEDFLGQPSMETHHVYDAEIIKSKGDYVQVKMTADYFGNKIEKIFTLYGN
                     SPLLEIRYALTFKNPEANVIG"
     gene            complement(2319..2813)
                     /locus_tag="VGM24_13285"
     CDS             complement(2319..2813)
                     /locus_tag="VGM24_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY4156406.1"
                     /translation="MLNLSIRPLEARDNAEIASIIREVLTEFGANKPGTVYADPATDD
                     LFGLFRMPRSYYAIAEVEGRIIGGSGVFPTPALPEDYCELVKLYLVPEARGLGIGRRL
                     MNICFEKAGEFGFKRIYLESMPELRNALGLYEKAGFTYLPGPLGKSGHYGCGLWMTKD
                     LFNS"
     gene            complement(2807..3616)
                     /locus_tag="VGM24_13290"
     CDS             complement(2807..3616)
                     /locus_tag="VGM24_13290"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY4156407.1"
                     /translation="MEQNKNYFETNRKIWNEKTEIHIRSAFYDNKSFLEGKSSLKSIE
                     LNLLGNLEGRSVLHLQCHFGQDSISMSRLGAKVTGIDLSDQAIRRAREFATQTGTQTK
                     FICCNIYDLPDHLDEQFDIVFTSYGTITWLPDLEKWAGIVSQYLKPNGRFILVEFHPV
                     VWMFDDEFDKVGYSYFNAGPIIEKEKGDYAEPEALAGQEFVGWNHGMSEILNSLINNG
                     LEINSFNEFDYSPYNCFRNTVEAEPGKYRIGHLGNKIPMLYSIGATKKRRC"
BASE COUNT          944 a         1001 c          802 g         1045 t
ORIGIN      
        1 cccgataacg tttgcctccg gatttttaaa agttagggca tagcggattt caagcagggg
       61 cgagttgccg tatagcgtaa atatcttttc tattttattt ccaaaataat ctgccgtcat
      121 tttcacctga acataatccc ctttgctttt gatgatttcc gcatcataca catggtgtgt
      181 ttccatgctg ggctggccaa gaaaatcttc aaaccctccg taaggataat attcccttcc
      241 ctggcggaaa ggacgtttgt catcgtccgc cttttcgggc cataatttaa aaaagatatt
      301 gtccttcctg cttttcacaa tatattcaat aacccgcccg cccgtggcca gcagtgttac
      361 gagaacactg tcattttcca tccggtattc cgaaacgccg tcatggttca gatcaatcgt
      421 atacgcctgg gtttgtccct ccggttttcc aacccccaat tgactggaat aatcaagtcc
      481 caaagctgag actttaacgg tataattccc ctggggcagg gccagctcaa acagcatgtt
      541 ttgaaaagag ccgctagccg ccgttccgtt ctgccggctg gtaaaaaccg gctttgccgg
      601 attatttttt tcatacacct gtacttccac cgggtaggaa ttggccgtgc tgaaattatg
      661 aatggtgacc ggatacttta tttttggagc cagcccatac agcagccggt gaacggagat
      721 agccggcggc agatccagga tggccagggt tgctttcgtc ttttttcccc attgaaaccg
      781 gacggcaacc ggattggttt tgtccatggc ttctgccata ggaagtatcc tgatctctat
      841 attttttgtg ctgttcgccg gaagggccaa ttcagtgggt atggcagcag ccaatttcaa
      901 ttccgggggc aatttgattt caagagaacc ggaaaccgga tgatcatatg tatttgacag
      961 gctgatccga agcgtataat tatccatctc cgcctgcagg tcaatccggt cgccaaaata
     1021 ttcttccaga aaaacccggt cgagcaatag catttgcagc gcatggcggt attcagccgg
     1081 ccccatcatt tttccttcct ccagaatggc cacctcccgg cgatgcatga tcgcatccca
     1141 cattcccggt ttggttaatt tctctccctt ctttataaac aggaccatac tcgaaaccag
     1201 gccgtcctgc aatgaaccgg tagttgaaat aaacggaata ttttcattat ctatctgttc
     1261 cttattccct tctgttgcaa tatatccgtc ataatgatac gcatcggctg ccgtatcata
     1321 tacccgccgg aacacatatc cccctttttc atgtgcgaat ttgacccagg gctctctccc
     1381 gttcatatcc tggacctgca gtccgttata gtccttacag ccataaacag gcgaaaatcc
     1441 gacatcggca actaccggaa gatcgacttt cgctatccag gatgccagtt catccttttc
     1501 caggaattcc atatatccgc cgaaggtaaa ccagtcattc ccctcgttca tcccctttcc
     1561 gatgatctcc agcgggtgac cgggttcaca ggtgacgctt ttcaagaggg tttcagcacc
     1621 aaaccagcct ttatgcaggt catattcacg gagtattctg cttgtgtttc cgatcagctc
     1681 ttccaccttc ctgcgcatac cggaatcaat ggcacagacc acaaatatct tccggctccc
     1741 attgtccagg taaaaagcat ttacctccct gaagtccacc gcaccgtatt gatggctttt
     1801 caattcccgc ctccaggaac tgtccggaga aaattttccc attaatttct tcccggtctc
     1861 tgattccggg ctcgtaaata ccagcacatt cccggtacgg attttcgctt ccagctcact
     1921 taaggaaggc tcttctgccg cggagaactc ttccgacagc aggccctcca gaaatccttc
     1981 ctgcatctgc aaaccgggca agcccaactc ctccgctatg tcccgtttgc tttgcatgat
     2041 ggtcagcgac ggatccggag cggtcatttt aaaaaggttc ccgtagcgat accattggcg
     2101 gtaatcatca tgccagtaag tggtgtaccc gttaaattgc accttattcc ggatgctatt
     2161 ttcaagggga tgaaaggatc cgttataatt aaatccggac agcggctggg cgctcgtata
     2221 tccggcattc agaaaagagc aaaataaaaa taatttaacg gcagcgaaca gacgttttct
     2281 catatcagca attttagaat ccggataaag ttataggatt aggaattaaa aagatctttt
     2341 gtcatccaca aaccacatcc gtaatggccg gacttaccca ggggcccggg taaataagtg
     2401 aaaccggcct tttcgtagag gccaagggca ttgcggagtt ccggcatgga ttccaggtaa
     2461 attcttttaa atccgaactc cccggctttc tcaaaacaga tattcatcag gcgccggcca
     2521 attcccagac cgcgcgcctc cggaaccagg tataatttca ccagttcaca atagtcttcc
     2581 ggaagggcgg gcgttggaaa aacgccggaa cccccgatga tccgcccttc cacttcggca
     2641 atcgcataat aggaacgggg catgcggaac agcccaaaca aatcatccgt ggccggatca
     2701 gcataaacgg ttccgggttt attggccccg aattccgtca ggacttcccg gatgatcgaa
     2761 gcgatctctg cattatccct ggcctctaac ggccggatgg aaaggttcag catcttcttt
     2821 tttttgttgc cccgatagaa tacagcatcg ggattttatt tccgagatgc ccgatccggt
     2881 attttcccgg ttccgcttcc acggtgttcc ggaaacaatt atacggtgaa taatcaaatt
     2941 cattaaatga attgatttca agaccgttat taatcaggct gttcagtatt tcactcatgc
     3001 cgtggttcca gcctacaaat tcctgcccgg cgagcgcctc cggctcggca tagtcccctt
     3061 tttctttttc gattatgggg cctgcattga aataggaata acctacttta tcaaattcat
     3121 catcaaacat ccagaccacc ggatggaact caaccaggat aaaccgcccg ttgggtttca
     3181 ggtattggga aacaattccg gcccatttct ccagatcagg caaccaggta atggtcccgt
     3241 agctcgtgaa tacgatatca aactgttcat ccaggtggtc cggcagatca taaatattgc
     3301 aacaaatgaa tttcgtttgc gtgccggtct gcgtggcaaa ttcccgggcc ctgcggatgg
     3361 cctgatcgga taaatcaata ccggtaacct ttgcgccaag cctgctcatt gaaatactat
     3421 cctgaccgaa atggcattgc agatgtaaaa cgctcctgcc ttccaggttg cccagtaaat
     3481 tcaattctat gcttttcagt gaagattttc cctccagaaa actcttatta tcataaaacg
     3541 ccgatctgat atgaatttcc gttttttcat tccagatctt ccggttcgtt tcaaaataat
     3601 ttttattctg ttccatcttt aataaactgt tatcccgaca caaaaataga gaaagaggac
     3661 aattacccgg aagaatccgg actttgcggt gtcgatgaag atttggatat tacattcaca
     3721 tcatccgcct tgtactacct tctgcgcata ttcgtttggc ttggactgaa attctattgc
     3781 aggacgatcc gg
//
LOCUS       DASYCU010000660         4519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2462461, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000660 DASYCU010000000
VERSION     DASYCU010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462461"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VGM24_13295"
     CDS             complement(<1..467)
                     /locus_tag="VGM24_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY4156408.1"
                     /translation="MAEQITDTELLAMFREPATKEKAFGTLVKKYQERLYWHIRRMLV
                     EHEDTNDVLQNVFIRVWNALANFREDAQLYTWLYRIATNESLTFLEQQKRKATLSFND
                     LEAGLSNKIRADKDFDASKLEWKLQLAIQQLPDKQRVVFTLRYYDEMPYEEMS"
     gene            complement(502..1452)
                     /locus_tag="VGM24_13300"
     CDS             complement(502..1452)
                     /locus_tag="VGM24_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY4156409.1"
                     /translation="MITVTGQKDTRSGFGDGIVEIARKNPDIVALTADLAGSLKLNQF
                     IKEFPERFVQCGISEANMISIAAGMTIGGKIPYTTSFANFSTGRVYDQIRQSVAYSGK
                     NVKICASHAGLTLGEDGATHQILEDIGMMKMVPGMTVIVPCDYAQTRAATIAIAEFTG
                     PVYLRFGRPVWPVFTSGMDFKIGKAQLFTEGKDVSIFACGHLVWIALEAEKILREKGI
                     SAEVINIHTIKPLDTEAILKSIRKTGCAVSAEEHNILGGLGDSIAQTASKNFPVPQEY
                     VGTNDTFGESGTPAQLLEKYGLDAAHIVASAEKAIFRKNK"
     gene            complement(1486..2541)
                     /gene="galE"
                     /locus_tag="VGM24_13305"
     CDS             complement(1486..2541)
                     /gene="galE"
                     /locus_tag="VGM24_13305"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY4156410.1"
                     /translation="MKKILVTGGCGYIGSHTIIDLLENGFDVISVDNNSRSNPRILEG
                     VEKITGKKIKNYKVDLCNFDDTFAIFHENPDIAGIIHFAAFKSVEESVSFPLMYFENN
                     LMSLINLLKCVQEFNVPHFVFSSSCTIYGNPAEIPVSETTPPKPAESPYGYTKQMGEQ
                     IINEFFKSTDLNAILLRYFNPVGAHPSAIIGELPVGRPANLVPAITQTAIGKLPQMQV
                     FGNDYPTRDGSCIRDFIHVCDIAHAHTLSLQYLIQGKGTKKCKVFNLGSGTGVTVLEA
                     IHAFEKVSGVSLNYVIGPRRAGDVIAIYANNDLARNELGWNPKFSLEDMLSTAWRWEQ
                     KIKMDETFYNSKFSELN"
     gene            complement(2558..3421)
                     /gene="rfbA"
                     /locus_tag="VGM24_13310"
     CDS             complement(2558..3421)
                     /gene="rfbA"
                     /locus_tag="VGM24_13310"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEY4156411.1"
                     /translation="MKGIILAGGSGTRLHPITLAISKQLMPVYDKPMIYYPLSTLMMA
                     GIREILIISTPNDLPLFKKLFEDGSRYGLEISYAEQPQPDGLAQAFVIGEDFIGKDPV
                     ALVLGDNIFYGAGLGDQLTANSRPDGGIVYAYQVSDPERYGVVEFDEGMKVVSIEEKP
                     AKPKSNFAVPGLYFYDNEVVAIAKGLKPSPRGEYEITDINKEYLKRGKLRVSIMARGT
                     AWLDTGTFDSLMQATQFVQVIEQRQGIKIACIEEIAYRKKFITKEQVKAIAQPLLKSG
                     YGQYLLDLINT"
     gene            complement(3471..4511)
                     /locus_tag="VGM24_13315"
     CDS             complement(3471..4511)
                     /locus_tag="VGM24_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY4156412.1"
                     /translation="MTFLVIIIILALIFDFINGFHDAANSIATIVSTKVLTPFQAVLW
                     AAMFNFIAFFISKYLIGEFKIGNTIASSVHKEFITLEVIFSGLIGAIAWNLLTWWFGI
                     PSSSSHTLIGGFIGAAIAHAGTLSKHGLDVINYAKVIPTFLFIFLAPVIGMIIALVIT
                     ITIVNICRRANPYRADKWFRRLQLVSSALFSLGHGGNDAQKVMGIIGAAMIFYQHGRG
                     VDISFNQFVDQNMWVPLASFTAIALGTLSGGWKIVKTMGTRITKVTPLEGVSAETAGA
                     ITLFLTEKLGIPVSTTHTIAGSIIGVGATKRLSAVRWGVTINLLWAWILTIPVSALFS
                     AIIYYLLVLIRK"
BASE COUNT         1095 a         1090 c         1143 g         1191 t
ORIGIN      
        1 tggctcattt cttcataagg catttcgtca taatatcgta gcgtaaatac aactcgttgt
       61 ttatcaggta gttgctgaat agctaattgc agtttccact ccagtttgct ggcgtcaaaa
      121 tccttgtcgg cccggatctt attgcttaac ccggcttcca ggtcattgaa ggaaagggta
      181 gcctttcgtt tctgctgttc aagaaaagtg aggctttcat tggtggctat ccggtaaagc
      241 caggtgtaaa gctgcgcatc ttcccggaaa ttggccaggg cattccagac acggatgaat
      301 acattttgca ggacatcgtt agtatcctca tgctccacca gcatgcggcg gatatgccag
      361 tagagccgtt cctgatattt ctttaccagg gtaccgaatg ctttttcctt tgttgccggt
      421 tcacggaaca tggccaataa ttcagtatcc gttatttgtt ccgccatcgg gttagccaga
      481 ttaattacag ggatcaaacc gtcatttgtt tttccggaaa atggcctttt cagctgaagc
      541 cacgatatgc gcggcatcga gcccgtattt ttccaatagc tgggcaggcg ttccactctc
      601 gccaaacgta tcattcgtgc ccacatactc ctggggaacg gggaaatttt tggaggctgt
      661 ctgcgcgatg ctgtcaccca gtcccccgag gatattgtgc tcttctgcag aaacggcgca
      721 tccggttttg cggatggatt ttaatatggc ttctgtatcc aggggtttaa tggtatgaat
      781 gtttatcact tccgcggaaa tgcctttttc cctcaggatt ttttctgctt ccagggcgat
      841 ccagaccagg tggccgcagg caaagatgga aacatccttt ccttcggtaa aaagctgggc
      901 ctttccaatt ttgaaatcca tcccggaggt aaaaaccggc cagaccggcc ttccaaagcg
      961 cagatatacc gggccggtaa attccgctat ggctatggtg gccgccctgg tctgggcata
     1021 atcacagggc acgatcacgg tcatcccggg caccattttc atcatcccga tatcttccag
     1081 gatctggtgg gtggctccat cttcccccag ggttaatccg gcgtgggaag cgcagatctt
     1141 tacatttttt ccggaatagg ccacggactg ccggatctgg tcatacacgc ggccggtaga
     1201 aaaattggca aagctggtgg tatacgggat ttttccgccg atcgtcattc cggccgcaat
     1261 gctgatcata ttggcttctg aaataccgca ctggacaaac cgttcaggaa attccttaat
     1321 gaactgattg agtttaagtg agccggccag atcggccgtt agcgcgacga tgtcggggtt
     1381 cttccgggct atttccacaa tgccgtctcc aaagccgctc cgggtatctt tctgtcctgt
     1441 taccgtaatc attttgttgc tgattgtata aaatataaat ggttattaat tgagttctga
     1501 aaacttgctg ttataaaagg tctcgtccat tttaattttc tgttcccagc gccaggcggt
     1561 ggacaacata tcttccaatg aaaatttcgg gttccagccc agttcattgc gggccaggtc
     1621 gttattggca taaatggcta tgacgtctcc ggccctgcgc ggaccgataa catagttaag
     1681 agaaaccccg ctgacttttt caaaagcatg aatggcttcc agcacggtta cgccggttcc
     1741 gctgcccaga ttaaagacct tgcacttttt ggttcccttt ccctggatca ggtattggag
     1801 ggaaagggtg tgggcgtgcg caatatcaca aacatggatg aagtcgcgga tgcaggagcc
     1861 gtcgcgcgtc gggtaatcat ttccaaaaac ctgcatctgc ggtaattttc caattgccgt
     1921 ttgcgtaatg gcgggcacga ggttggcggg cctgcccacg ggaagttcgc caatgatggc
     1981 ggacgggtga gcgcccacgg ggttaaagta tctcagcagg atggcattca gatcagtgga
     2041 tttaaaaaat tcatttataa tctgctcccc catttgtttg gtatatccgt aaggcgattc
     2101 cgccggtttg gggggagtgg tttcggatac cgggatttct gccggattgc catagatggt
     2161 acaggaagaa gagaatacaa aatgaggaac attgaattcc tgtacgcatt tgagcaggtt
     2221 gatgagcgac atcaggttgt tctcaaaata catcaggggg aaactgaccg attcctctac
     2281 ggatttaaat gctgcaaaat ggataatgcc cgcgatgtcc ggattttcat gaaagatggc
     2341 aaaggtgtca tcaaaattgc aaaggtccac cttgtagttc ttgatctttt tccccgttat
     2401 tttttccacc ccttccagga tacgggggtt ggagcgggag ttattgtcta cggagatcac
     2461 atcaaatcca ttctctaaaa gatcgataat ggtgtgcgac ccgatatatc cgcagccgcc
     2521 ggtaacaaga atttttttca tagttgagtc agcaaaatta ggtattgatc aaatccagca
     2581 ggtattgtcc gtacccgctt ttcaacaggg gttgtgcaat ggcttttacc tgttcttttg
     2641 taataaattt tttccggtag gcaatttctt ctatgcaggc gatcttgatg ccctgccgtt
     2701 gttctatgac ctgcacaaac tgggttgcct gcatcaggga atcaaacgtt ccggtatcca
     2761 gccaggccgt tccgcgggcc ataatggata cccggagttt tcctcttttt aaatattcct
     2821 tattaatatc cgtgatttcg tattctccgc ggggactcgg tttcagccct ttggcgatgg
     2881 ccaccacctc gttatcataa aaatataatc ccggcaccgc aaaattgctc tttggttttg
     2941 ccggtttttc ttctatggaa accaccttca tcccctcgtc aaattccacg acgccataac
     3001 gttccggatc actcacctgg taggcataca cgattccacc gtcgggacgg gaatttgcgg
     3061 taagctgatc gcccagaccg gccccgtaaa agatattgtc tcccagcacc agggccacag
     3121 ggtctttccc gataaaatcc tctccgatca caaaagcctg cgccaggccg tccggctgcg
     3181 gctgctcggc ataggagatc tccaaaccgt agcgggaacc gtcttcgaat aattttttga
     3241 agagaggcag gtcattggga gtggaaatga taagaatctc ccggattccg gccatcatca
     3301 aagtggacag gggataatag atcatcggtt tatcgtaaac cggcatgagt tgcttgctga
     3361 tggcaagcgt aatgggatgt aatcgggtgc cagatccacc ggccagaata attcctttca
     3421 ttaagccctg ttttaaaccg gcgcaaagta cccaaaaaac ggcacatatt ttatttccgg
     3481 ataagcacca acagataata tatgatggcg gagaacaggg cgctgaccgg aatggtcagg
     3541 atccaggccc agagcaggtt aatggtcact ccccatctta cggccgagag tcttttggtg
     3601 gcgccgacgc cgatgatgga tccggcaatg gtatgcgtgg tgctcacggg gatacccagt
     3661 ttttcagtaa ggaaaagggt gatggcgcct gcggtttctg cgcttacccc ttccagcggg
     3721 gtcactttgg tgatccgggt gcccatggtt tttacgatct tccaccctcc gctgagcgtg
     3781 cccagggcga tggcggtaaa acttgccagc ggaacccaca tattctggtc tacaaattga
     3841 ttaaagctga tatcgacgcc tctcccgtgc tggtaaaaaa tcatggctgc gcctataatt
     3901 cccattactt tttgtgcgtc attacccccg tgccccaggc tgaataaggc ggaagacacc
     3961 agctgcaaac gccggaacca tttgtccgcg cggtatggat ttgcccgccg gcagatattg
     4021 acgatcgtta tggtaatgac cagcgcaatg atcatcccga tgacgggggc aagaaaaata
     4081 aaaaggaagg tgggtatcac cttggcatag ttaattacgt caagaccgtg tttgcttagg
     4141 gtgccggcat gggcgatggc agcgccgata aagccaccga tcagggtatg ggaagaactg
     4201 gaaggaatgc caaaccacca ggttaacagg ttccaggcaa tggcgccgat cagtccggaa
     4261 aagatcactt ccagcgtgat aaactctttg tgaacggaag aagcaatggt gttcccgatc
     4321 ttaaactcgc cgatcaggta ttttgaaata aagaatgcga taaagttgaa catggcggcc
     4381 cacaaaacgg cctggaacgg cgtcagcacc ttggtggaaa caatggtggc tatcgagttg
     4441 gccgcatcat ggaaaccatt gataaagtca aaaatgagag ccaggatgat aataattacc
     4501 aggaaggtca tgcgtttga
//
LOCUS       DASYCU010000661         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_2608634, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000661 DASYCU010000000
VERSION     DASYCU010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2608634"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1356
                     /locus_tag="VGM24_13320"
     CDS             37..1356
                     /locus_tag="VGM24_13320"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY4156413.1"
                     /translation="MKKRVAALLITLISCLPGCKQKAGNQASFNNKDISRVISYMTGL
                     MLHDITNPPLAARFFSYACLAGYEVVSQNDHSVRKMHSILNAYPDLQKPDSISGYSYP
                     LAALLAMMETAAKMQPSGKLFAGYEKRFLDSCSLAGYPEELIENSKRYALAVSKQILA
                     YAKADGYNKISNLPRYTPLNKDSSWYPTPPAFINAVEPYFNTIRSFTLDSASQFKPRR
                     PVSFSTDKHSAFYKLMMDNYREILNQPQEHKDIAGFWDCNPFAVQDEGHLMVGLKKIS
                     PGAHWLGIAGIACEKANTSFAKTMEIYTMVSITLTDAFICCWDEKYRSNRIRPETAIR
                     RYIDASWKPLLQTPPFPEYTSGHSVVSASSSVILTHYLGNNFSFRDTVEEAYGLKPRS
                     FTSFHQAADEAAISRFYGGIHYTDANIIGKELGNQIGDWVLLKVNKH"
     gene            1353..>1855
                     /locus_tag="VGM24_13325"
     CDS             1353..>1855
                     /locus_tag="VGM24_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY4156414.1"
                     /translation="MNYKVTISDIAKELNTTPATVSRALSNHPAISSKTKESVQKAAK
                     RLNYKRNRIASSLRSGKTHVIGVIIPSAEINFFGSVVHGIESIANSNGFNVLIYQSNE
                     NRDHEVRGIETFISARVDGILASIAKNTLDYSHFLDIKARNIPLVFFDRANIDLGIPS
                     VVIDDYKG"
BASE COUNT          509 a          466 c          430 g          450 t
ORIGIN      
        1 tcaaaaggaa ataaaagcat ataaatcgaa gtagaaatga aaaaacgggt tgcagcctta
       61 ttgataactc tgatctcctg tttgcccgga tgtaaacaaa aggccggaaa ccaggcatcc
      121 ttcaataata aagacatcag ccgggttatt tcctatatga ccgggctcat gcttcatgat
      181 attaccaatc ccccgctggc cgcccggttt ttttcctacg cctgtctggc gggctatgag
      241 gtcgtatccc agaacgatca ttccgtcaga aaaatgcaca gcatcctgaa tgcctatccg
      301 gatctgcaaa agccggattc catctccggt tattcctatc cgttagctgc cctgctggcc
      361 atgatggaaa cagcggcgaa aatgcagccg tcgggaaaac tttttgccgg ttacgagaaa
      421 agatttctgg attcctgcag tctcgcaggt tacccggaag agctgattga aaattcgaaa
      481 agatatgcgc tggcggtcag caaacagatc ctcgcttatg ccaaagccga tggttacaac
      541 aagatcagta acctgccgag atatacgcca ttgaacaagg acagcagctg gtaccccacc
      601 ccgccggcat tcatcaatgc cgtggaacct tatttcaata ccatccggtc atttaccctg
      661 gattctgcct cgcaatttaa accccggcgt cccgtttcgt tttcgacgga taaacactct
      721 gctttttata aactgatgat ggataattac cgggaaatcc tgaatcagcc ccaggagcac
      781 aaggatatcg ccggtttctg ggattgcaat ccgtttgccg ttcaggacga aggacatttg
      841 atggtggggt tgaaaaaaat ctccccgggc gcacactggc tcggaatagc cggcatcgcc
      901 tgtgaaaagg cgaatacaag tttcgcaaag accatggaaa tatataccat ggtatccata
      961 accctgacgg atgcctttat ctgttgctgg gatgaaaagt acaggagtaa ccggatacgg
     1021 cctgaaacag ccattcgccg ctatatagac gccagctgga agcccctgct gcagacccct
     1081 ccgtttcccg aatacacaag cggccattcg gtggtctcgg cttcctcttc ggttatactg
     1141 acacattact tagggaataa tttttctttc cgggacacgg tggaagaagc gtatggactg
     1201 aagccgcgct ccttcacttc ttttcatcag gcggcggacg aagccgccat atcccgcttt
     1261 tacggaggaa ttcattatac ggacgccaat atcattggca aggaactggg gaatcagatc
     1321 ggggattggg tcctgcttaa agtaaacaag cattgaatta taaagtaacc atatcggata
     1381 ttgccaaaga gctcaacacc acgccagcta cggtttcccg ggcattaagc aaccatccgg
     1441 ccatcagctc caaaactaaa gaatccgttc agaaagcagc caagcggctg aattataaaa
     1501 ggaaccgcat cgcttcatcg ctccggtccg gcaaaacaca tgtgatcggg gtgatcattc
     1561 cgagtgccga aatcaatttt ttcggttcgg tcgttcatgg catcgagagc atcgccaact
     1621 caaacgggtt taatgttctt atttatcagt ccaatgaaaa cagggaccat gaagtaagag
     1681 gaattgaaac gttcatcagc gcccgggtgg atggcatcct ggcttccatt gcaaaaaata
     1741 ccctcgacta ttcccatttc ctcgatataa aagccaggaa tattccgctg gttttctttg
     1801 accgggccaa tattgattta ggaattccct cggtggtgat cgatgattat aaggg
//
LOCUS       DASYCU010000662         5516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_4201801, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000662 DASYCU010000000
VERSION     DASYCU010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4201801"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VGM24_13330"
     CDS             <1..779
                     /locus_tag="VGM24_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY4156415.1"
                     /translation="KLPSLKYIGVLATGYNIIDTETAKKNNVVVANVPGYGTASVVQM
                     TFSLLLELCLHVQKHSDAVRSGKWSASPDFCFWDFPLIELAGKTMGIIGFGNIGEKVG
                     DLATAFGMNLLGNRRNPTEQSHRKNFRWASVDEILEASDVLSLHCPLSPETEGFLNRQ
                     SLKKMKKSAFLINTSRGQLVVDEDLAEALNKDLIAGAGIDVLSVEPPPPDNPLLRAKN
                     CIITPHIAWATKEARARLMNTAVNNLSAFIAGSPVHVVNR"
     gene            785..1915
                     /locus_tag="VGM24_13335"
     CDS             785..1915
                     /locus_tag="VGM24_13335"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY4156416.1"
                     /translation="MHILIAPNAFKNSLPATAAAEAILAGLKQSRLRFSHECFPIGDG
                     GNGTAELLIRNQRGRMISGIAHDPLGRKIRASFGLIREDRTAVIEMADAAGLQFLKQD
                     ELDPLKTSSSGCGEMIRQALDQGVRKIILGMGGSATVDGGTGILRALGIRFLDACGNE
                     LPDRPESLINLDAIDLSGMDKRILEAELIILCDVNNPLLGSQGAAAVFGPQKGATKEG
                     VKKLEAALTKFQELTLRQTGKEIASLRSGGTAGGAAAGLHAFLNARLVSGIEYFLELT
                     GFDEALRKAGLLITGEGAIDEQTLQGKAPFGVASRAKERNLPVIALAGKVPLEKSAAL
                     DQYFDVLLAIGNEPADLTSAMKETTENLTRISKSIGNLLALP"
     gene            2067..2753
                     /locus_tag="VGM24_13340"
     CDS             2067..2753
                     /locus_tag="VGM24_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156417.1"
                     /translation="MNKEWIQAPLNGTHKGPPLGMLAIVYVVLFAVSTVIMPILTHGA
                     PMPMPYGPAELSRHYYLQFPDALRINAWLQFGSIIPLGIFTAAITSRLKFLGINASGV
                     NIALFGGIGASLFLGFSGLASWVQSQPGISNDIGSMRVVQLSGFFCGGVAHVVLFGLL
                     LAGVSVTARFRGLISSGLFWFGMITAAFAELSSLSIIFPILSIFIPLGRYPGFIWMII
                     VGFKLPLSVK"
     gene            2760..3779
                     /locus_tag="VGM24_13345"
     CDS             2760..3779
                     /locus_tag="VGM24_13345"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY4156418.1"
                     /translation="MTGISRNRYFTPSIHLLIWGLLLAIPAVLSIGVSIKTGLPPHFF
                     LLTNLYHIGLFYLNANFLYPRLLNRRFWWLYFPVIAGVIAASWYMKLFFIGLADPGFE
                     CNDFNCRIIFFGTIPFLFASFIFRLLVNRIREEKAKKEAEAERLASELKFLRSQVSPH
                     FLFNMMTNMVALARQKSDLLEPSLIKLSGLLRYMLYEKDEEKFTLSKELEYLESYIEL
                     QQLRFGEGVDLEMQKEAGDDEMKCRIEPMLLVPFVENAFKHGIGLVENPFIKIALRVK
                     DAHLFFSVSNNYSRDEFSKDKNSGIGLLNVQNRLKLLYPDKYSLRIRDNGEIYGVELN
                     LDLSC"
     gene            3773..4477
                     /locus_tag="VGM24_13350"
     CDS             3773..4477
                     /locus_tag="VGM24_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY4156419.1"
                     /translation="MLNCIAVDDEKYVLDLLVDNIQKVPFLQLKARCRNAMEAAAVLR
                     EEEIDLIFLDIQMPGLNGLQFLRSLQHPPMVIFVTAYKDYAFDGFDLNAVDYLLKPVS
                     FERFLKACNKAYDLYKLQQQPPLQKGEAPAYFFVYVEYNQVKISIPDILYIEGLKDYV
                     KIFLGSSPKPVITRMSLKELEEKLFSSRFVRIHKSYIVASDKVTAIKRDLICIGPTEL
                     PLSETYKPAVEKMLAL"
     gene            4658..4873
                     /locus_tag="VGM24_13355"
     CDS             4658..4873
                     /locus_tag="VGM24_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156420.1"
                     /translation="MKKIVILLASAILLNTGIKAEDASPSLMQAYVSRLEKVSSINEY
                     QQLAHDFLRFARIVQPLYEPGPGLEPG"
     gene            4842..5060
                     /locus_tag="VGM24_13360"
     CDS             4842..5060
                     /locus_tag="VGM24_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156421.1"
                     /translation="MNLALALNPDNPRAEYLAGWEKYYTPKMWGGDKLKAKELLESAR
                     QKLDKDPARGLAPHWGKKEVDELLQKIK"
     gene            5310..>5516
                     /locus_tag="VGM24_13365"
     CDS             5310..>5516
                     /locus_tag="VGM24_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY4156422.1"
                     /translation="MAEKSLTYKKRILKQLYFYDTLSSADLSAQINLSLPITTKMVNE
                     LIEEGHVLETGYAPSTGGRRPLMYS"
BASE COUNT         1394 a         1338 c         1386 g         1398 t
ORIGIN      
        1 aaaaacttcc ttccctgaaa tacatcgggg tcctcgccac gggttataat atcatcgaca
       61 cggaaacggc aaagaaaaat aatgtggtcg tggcgaatgt tccgggatac ggcaccgcat
      121 ccgtggtgca aatgacattc agcctgttgc tggagctttg ccttcatgtg caaaaacata
      181 gcgatgcggt caggtcggga aaatggtctg cttctcccga tttttgtttc tgggattttc
      241 cgctgatcga actggccggc aagaccatgg gtattatcgg ttttgggaat atcggtgaga
      301 aagtcggaga cctggctacg gccttcggca tgaacctgct gggaaacagg agaaacccga
      361 cggagcagtc gcaccggaaa aatttcaggt gggccagcgt cgacgaaatc ctggaagcct
      421 ccgatgtgct gagcctccat tgcccgcttt cacccgaaac agaaggattt cttaaccggc
      481 aaagcctgaa gaagatgaaa aaaagcgctt tcttaataaa tacatcgaga ggtcagctcg
      541 tcgtggacga agaccttgcc gaagcattaa ataaggatct tattgccggg gcaggaatag
      601 atgtgctttc tgttgaaccg ccgccgccgg acaatcccct gctgcgggca aaaaattgta
      661 tcatcactcc ccatattgcc tgggcaacca aagaagcaag ggcgcggtta atgaataccg
      721 cagtgaataa cctgagcgcc tttatcgccg gaagcccggt tcatgtggtg aacagataac
      781 ggacatgcat atactcattg cccccaatgc atttaaaaac agtctcccgg ccaccgcggc
      841 agcggaagcc attctggccg ggctcaagca aagccggctc cggttcagcc atgaatgttt
      901 tcccatcggc gacggaggga acggaaccgc cgagttgctg atcagaaacc agcgcggccg
      961 catgatctcc gggattgccc atgatcccct cggaagaaag atcagggcat cattcggatt
     1021 gatccgggaa gaccggacag cagttattga aatggccgat gcggccggct tgcaattttt
     1081 aaaacaggat gaactggatc cgttaaagac cagttcctcc ggctgcgggg aaatgatccg
     1141 gcaggccctg gatcagggag tgcgtaaaat aatcctgggc atgggcggct ccgctacagt
     1201 tgacggcggc accggaatcc tgcgggcact gggcatccgc tttctggatg cctgtggcaa
     1261 tgaattgccc gaccggccgg aaagcctgat taacctggat gcgatcgatc tatcgggaat
     1321 ggataaacga attttggagg cggagctgat catcctttgt gatgtgaata atccgctcct
     1381 gggcagccag ggagcagcag cggtttttgg cccgcagaag ggcgcaacaa aagaaggggt
     1441 aaaaaagctc gaagccgcat taacgaaatt tcaggaattg acccttcgtc aaaccggtaa
     1501 ggagatcgct tctctgcggt cggggggaac cgccggcggg gctgcggccg gactccatgc
     1561 ttttctgaat gcccgcctgg tgagcggaat tgaatatttc cttgaattga ccggttttga
     1621 tgaagcgcta aggaaggccg gtctcctgat caccggggaa ggagccattg atgaacagac
     1681 cctgcaggga aaagctccgt ttggtgtggc ttccagggcc aaggaaagaa accttccggt
     1741 cattgcgctg gcgggcaaag tacccctgga gaaaagcgcg gcgctggatc aatattttga
     1801 cgtgctgctc gccatcggaa atgaaccggc ggacctgacc tccgccatga aagaaaccac
     1861 tgaaaattta acccggatct cgaagtcgat agggaattta ctggcccttc cctgaaccgc
     1921 ttccctccac aagccggcag acggccggtt tccctccgca atttcacttt tataagcagg
     1981 gaatgtctac acatttccgg ctttggtcta cgcttgcccc ataacggcgt ttaagcggac
     2041 tatttttatt gattaaaatt ttcattatga acaaagaatg gattcaggct ccgctgaatg
     2101 gcacgcacaa gggacctcca ttaggcatgc tggccatcgt gtatgtggta ttgtttgccg
     2161 tcagcaccgt catcatgccg atattaaccc atggagcgcc gatgcccatg ccctatggcc
     2221 cggcagaact ttcacgccat tattatttac agtttccgga tgcattaaga ataaatgcct
     2281 ggttgcagtt tggttccatt attccccttg gaatttttac cgccgccata accagccgcc
     2341 tgaagttcct gggcataaat gcctccggtg tgaacattgc tttattcggt ggaatcgggg
     2401 cgtcattatt tcttggattt tccggcctgg cctcctgggt gcagtcccag cccggtattt
     2461 caaatgacat cggcagtatg cgggtggttc agctgtccgg atttttctgc ggaggggttg
     2521 cgcacgtcgt gctgtttggg ttactcctgg ccggcgtttc cgtcacggcc cggttccgcg
     2581 gcctgatatc gtccggctta ttctggtttg gaatgatcac tgcggccttt gcggaactat
     2641 ccagcctgag catcattttt cccattcttt ccatatttat tcctttgggc aggtatcccg
     2701 gatttatctg gatgatcatc gttggattta agcttccttt gtcagtcaaa taaccgaaga
     2761 tgaccggcat atcccgcaac agatacttca cgccctccat acacctcctc atctggggtt
     2821 tgctgctggc cattccggcg gtattgtcca tcggggtttc gataaagacc ggtcttcccc
     2881 cgcacttctt tttgctcacc aacctgtatc atatcggctt gttttacctg aatgccaatt
     2941 tcctgtatcc ccgcttgctg aaccgcaggt tctggtggtt gtatttcccg gttatcgcag
     3001 gggtcattgc ggcttcctgg tatatgaaat tatttttcat aggactggcc gatccgggtt
     3061 ttgaatgcaa tgactttaac tgccgcatta tttttttcgg caccattccc tttttattcg
     3121 cttcttttat tttccgactg ctggtcaacc ggatcaggga ggaaaaagca aaaaaggaag
     3181 cagaggccga acggctggct tccgagctaa aattcctgcg ttcccaggta agccctcatt
     3241 ttctttttaa tatgatgacc aatatggtcg ccctcgccag gcaaaaatcg gatctgctcg
     3301 aaccttcact aattaaactt tccggcttat tgcgctatat gttgtatgaa aaggacgaag
     3361 agaaatttac cttaagcaaa gaactggaat acctggaaag ctatatcgaa ctgcaacagc
     3421 tgcgcttcgg ggaaggggtg gacctggaaa tgcagaagga ggccggcgac gacgaaatga
     3481 aatgccggat tgagccgatg ctgctggtgc cttttgttga aaatgcgttc aagcacggga
     3541 tcgggctggt ggaaaaccct tttataaaga tcgcgcttcg cgtcaaggat gcgcatctgt
     3601 ttttttcagt cagtaataac tacagcaggg acgaattttc aaaggataaa aattccggga
     3661 tcgggttgct caatgtgcag aaccggttga agttattgta cccggacaaa tattcactcc
     3721 gcatccggga taatggcgaa atttacgggg tggaattaaa cctggatctt tcatgctgaa
     3781 ttgtattgcc gttgacgatg aaaaatacgt gctggatctt ctggtcgata atattcagaa
     3841 ggtcccgttt ctgcaattga aggcgaggtg ccggaatgcc atggaagccg ctgccgttct
     3901 ccgcgaggag gaaatagacc tgatttttct ggatatccag atgccgggac tgaacggcct
     3961 gcaattcctg cgctccctgc agcatccgcc catggtcatt tttgtgaccg catacaagga
     4021 ctatgcattt gacggattcg atttgaatgc cgttgattat ttattgaagc cggtttcctt
     4081 cgagcgtttt ttaaaagcct gcaacaaggc ctatgacctg tataaattgc aacaacagcc
     4141 gccgctacag aaaggagaag cgccggctta cttttttgtc tacgtggaat acaaccaggt
     4201 taagatcagc atccccgata ttctttatat tgaagggctc aaggattatg ttaagatttt
     4261 tctcggttct tctcccaaac ccgtcattac gcggatgagc ctgaaggaac tggaagaaaa
     4321 gcttttctcc agccggttcg tccgcataca taaatcgtat atcgtcgcct cggataaggt
     4381 gaccgcgatc aaacgcgacc tgatctgcat cggtcccacc gagcttccgt taagcgagac
     4441 ctataagccc gcggtcgaaa aaatgctcgc tttgtaaatc gcatcatctt tcccgaatat
     4501 ctattcaacc cgcctgctgg tagattctgg atctcccccg gtttttttgt gaaatagttt
     4561 tacggtgtaa ataaaaacag atgaaatcgc atcgacacgg gaaatgattt catcgatcgg
     4621 aacctgtaat tcatttaatc acaaaaaaaa taataaaatg aaaaagatcg ttatcctgct
     4681 ggcatccgcc atccttttaa ataccggaat aaaggcagag gatgcttccc cgagcctgat
     4741 gcaggcttat gtttcgcggc tggagaaagt ttcttccatc aatgaatatc agcaactggc
     4801 tcatgatttt ctgcgtttcg cgaggatcgt ccaaccgcta tatgaacctg gccctggcct
     4861 tgaacccgga taatccgagg gcggagtacc tggcgggttg ggaaaagtat tataccccga
     4921 aaatgtgggg cggcgataag ctaaaagcca aagaattgct cgagtctgcc aggcaaaagc
     4981 tggataagga ccccgcccgg ggcctggccc cgcattgggg gaagaaagag gtggacgagc
     5041 tgctccagaa aatcaaatag gctccggccg ggacccggcc tccattaaat cctgcccttt
     5101 tccaccggat ggggcaggat ttaaataccg gtagacactg attctcagct aaatggctat
     5161 tcccttccac caaaaagacc aatccataag attttacaga actatcacca cttatcatta
     5221 aaatggaata ctttaaataa atttttaaaa agtttaatta cttaacatac atttatcgaa
     5281 attttcacca ggaaataaca agacctcgaa tggctgagaa aagtcttacc tataaaaaaa
     5341 ggatacttaa gcagctttat ttctatgata ccctgtccag tgcagatttg agcgcccaga
     5401 taaatttgag tttgccaata acgacaaaaa tggtgaatga attgattgaa gaaggtcatg
     5461 ttttggaaac gggttatgct ccttccaccg gtgggagaag gccattgatg tattcg
//
LOCUS       DASYCU010000663         5215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_1158389, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000663 DASYCU010000000
VERSION     DASYCU010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158389"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..3240
                     /locus_tag="VGM24_13370"
     CDS             73..3240
                     /locus_tag="VGM24_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEY4156423.1"
                     /translation="MIADTFIKRPVTAIVISIVIVLAGILALLTLPVSQYPEITPPTV
                     SVTSTFTGADAQTVEETVTTPIESQINGTPGMAYLQSNSTSDGQSNITVTYDVGADVN
                     IGMLDVQNRVGIATPQLPDEVKRLGVITRKRNPSILLLVAIYSPNGTHSVPFMDNYSN
                     IFVRDVLLRVPGVGDVIKRADDFSMRIWLKPDKLAQLGLSASDVTSALQEQNVQISAG
                     SVGAPPQANQQAFEFTVVANSRLTTEKQFDDVIVRANPKDGSLVHLSDVARVQLGKFS
                     YSANSFVDGKRSSYLLVYQAPGSNALETAKGVYAAMEKLKKSFPADIDYVVPFESVSV
                     VQVSIHEVVQTLLEALGLVVLVVFLFLQSWRATLIPILAIPVSIIGTFIFFIPLGFTI
                     NTLTLFGFVLAIGIVVDDAIVVVEAVQHYVDNEHLTAKEATVKAMKDISGPVVAIALI
                     LAAVFVPVGFIPGIVGRLYQQFAITIAISVLISAFIALSLTPALCTMLLRPMNLDKSS
                     KGFNQFFYRFNKWFTNVTNRYTHGVETWIKKAPYVIVLLACICVGAFLLFKAKPSGFI
                     PQEDEGRLYITYDLPEASSTNRSLVTLNKIMNIVQNTKGIAHFAALGGLNVITFASKP
                     NSGTIFTSLTPWDQRKDKSLQLSGIMATLQKKFAAIKEANVLVISPPAIPGLGRTGGF
                     SFELQQKESTDDIPHFEQVMHDFIGEINKRPEINRAYSFFTAKTPGYKLDVDRDQCKK
                     LGVSLTEVYNTIQTYLGSQYVNDFTIYGRNFHVVEQADSSYRGDIKQINNYYVRNMAG
                     TMLPLSSVVSYKLIESAPMISHFNIFRTAEINGDAKAGYSSGQALRALQEVAAKSLPT
                     GYGYEFAGLSREELKAGSSTLMIFALSIVFVFLFLAALYESWQVPFSVLLAVPIGLTG
                     AILTLTIIPHMVNNVYAQIGMITLIGLSAKNAILIVEFAKERVDRGLPIIQSTLDAVK
                     LRLRPILMTSLAFILGVLPLALATGAASVARSTIGWTVLGGMLAATSLAIFIVPVLFV
                     VITRFSYSKRKLNELKEHVVSGLTE"
     gene            complement(3327..4223)
                     /locus_tag="VGM24_13375"
     CDS             complement(3327..4223)
                     /locus_tag="VGM24_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156424.1"
                     /translation="MRFRSVYLSGKILAISALFFTTSCKKDIGNSLKSDDAAAASISD
                     SSTVADNAYNDVFVNAYYGYSDNLLTGGSGVLRQGGTTTNAVGVMNHIYLSCASYTVD
                     TTQGTGYPITLTLDFGADGCSNSIDSIRRQGKIIYVFTGPLVLPGSSVSATFDHYFVN
                     GYGLQGSCTITNTTTLAGFSFTSQVSNGIFTFPDLTNFHYSGNKTITLTAGSGTPYNV
                     LDDVYSITGNSNFSASDGTSLVSTVSTPLTKAFICPYVSAGVISFVFNQSLNGTIDFG
                     DGTCDHSAELAVGSITRQINLR"
     gene            complement(4370..4786)
                     /locus_tag="VGM24_13380"
     CDS             complement(4370..4786)
                     /locus_tag="VGM24_13380"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY4156425.1"
                     /translation="MSNRTFTMIKPDAFANGHSGAILDIIIKAGFKLTALKMTTLSEA
                     KAGEFYAVHQARPFYGELVGFMSRGPIVAAILEKENAVADFRKLIGATDPAKADPGTI
                     RQLFAASLGENAIHGSDSDENARIECDFFFSNLERI"
     gene            4875..>5215
                     /locus_tag="VGM24_13385"
     CDS             4875..>5215
                     /locus_tag="VGM24_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY4156426.1"
                     /translation="MQQITEIFPLLRTPKSMVIVMHQKPDADAMGSSLGLYHILVALG
                     HSVTVISPTNWADWLKWMKAADKVIDYELNTAKGNEALEKAEWIFCLDFNTLARSRKM
                     AEPIARSSAVR"
BASE COUNT         1284 a         1300 c         1298 g         1333 t
ORIGIN      
        1 cccgggcgga aatattccgg gaacgtccgc ggcagattca acaaaaggaa aataattgta
       61 acctgatata aaatgattgc agatactttt attaaaagac cggtaacggc cattgtgatt
      121 tcgatcgtta ttgtgctggc aggtattctg gctttactta cattaccggt gagccagtat
      181 ccggaaatta cgccacccac cgtatccgtt accagcacct ttaccggcgc cgacgcgcaa
      241 accgtggagg aaacggttac cacaccaatt gaatcccaga tcaacggaac gccgggcatg
      301 gcctatttac aatccaacag cacgagcgac gggcaaagca atatcaccgt cacctacgat
      361 gtcggggcgg atgtaaatat cggaatgctg gacgtccaga accgggtagg gatcgccacg
      421 ccgcagcttc ccgatgaggt aaaaaggctg ggcgtgatca cgcggaaaag aaatccaagt
      481 attttattac tggtggccat ttattctccc aatggcacgc attcggtccc tttcatggat
      541 aattactcca atatttttgt ccgggatgtg cttctgcgtg tgccgggtgt gggagatgtt
      601 atcaagcgcg cggacgattt cagtatgcgg atctggctga aacccgataa acttgcccag
      661 ctcgggcttt ccgcttcgga tgtcaccagc gccctgcagg aacagaatgt tcagatttcg
      721 gcaggttctg taggggcgcc cccgcaggca aaccagcagg catttgaatt caccgttgta
      781 gccaacagcc gcctcaccac ggaaaagcag ttcgacgatg tgatcgttcg tgccaacccg
      841 aaagacggtt cgctggttca cctgagtgat gtggcccgcg ttcaactcgg aaaattcagt
      901 tattctgcca attcttttgt agacggaaaa agatcctcct atctcctggt ttatcaggcg
      961 cccggaagca atgccctgga aacggccaaa ggcgtgtatg cggccatgga aaagctcaag
     1021 aaatcctttc cggccgatat tgactatgta gttccttttg aatccgtttc ggttgtccag
     1081 gtgtccattc acgaagtggt acaaaccctg ctggaagctc tcggactggt cgtgctggtc
     1141 gtttttcttt ttctgcaaag ctggcgcgcg acccttatac cgattctggc cattccggtt
     1201 tccattatcg gaacctttat attttttatt cccctggggt tcaccattaa cacgcttacc
     1261 ctttttgggt tcgtactggc gatcggtatt gtggtggatg atgcgatcgt agtggtggag
     1321 gccgtgcagc attatgtgga caatgaacac ctgacggcaa aggaggctac cgtaaaagcg
     1381 atgaaagaca tctccggtcc ggtcgttgcc atcgcgctga tcctcgccgc cgtattcgtt
     1441 ccggttggct ttattcccgg tatcgtcgga aggctgtacc agcagtttgc catcaccatt
     1501 gctatttccg tgctgatctc cgcgttcatc gccctttccc ttacgccggc tctctgcacg
     1561 atgcttttac gcccgatgaa cctggataaa tcttcaaaag ggtttaacca gtttttctac
     1621 cgtttcaata aatggtttac gaatgtgacc aaccggtata cccatggggt ggaaacctgg
     1681 ataaagaaag ccccgtatgt aatcgtgctg ctggcttgta tctgcgttgg cgccttcctt
     1741 ttattcaagg ccaagcccag cggctttatt ccgcaggaag atgaaggaag actctatatc
     1801 acctatgatt tgccggaagc ttcttccaca aaccggagcc tggtgaccct gaacaagatc
     1861 atgaatattg ttcagaacac aaaagggatt gcccattttg ccgcgctcgg cggactcaac
     1921 gtcataacct ttgccagtaa acccaatagc ggaaccatct tcacctccct gacgccctgg
     1981 gatcaaagaa aggataaatc cctgcagctg agtgggatca tggctaccct gcagaaaaaa
     2041 tttgccgcca tcaaagaagc caacgtgctg gtgatttccc caccggccat tccgggcctg
     2101 ggaaggactg gcggattcag tttcgaattg cagcaaaagg aaagcaccga tgatatcccg
     2161 cactttgaac aggtgatgca cgatttcatc ggggaaatca acaagagacc ggagatcaat
     2221 cgcgcctatt ctttcttcac cgccaaaaca cccggatata agctggatgt tgaccgcgac
     2281 cagtgcaaaa aactcggggt ttccctaacc gaagtctata ataccataca aacctatctc
     2341 ggcagccagt atgtgaatga cttcaccatt tacggaagga atttccatgt agtggagcag
     2401 gccgactcct cttaccgcgg agacatcaag cagatcaaca attattatgt ccgcaatatg
     2461 gccggtacga tgctgcccct gagctcggtc gtttcttata agcttattga aagtgctccg
     2521 atgatttccc actttaatat attcagaaca gccgagatca acggagatgc caaggccgga
     2581 tacagcagcg gtcaggccct ccgggccttg caggaggtag ccgccaaatc acttcctacc
     2641 ggttatggat atgagtttgc cgggctgagc cgggaagaat tgaaagcagg cagcagcacg
     2701 ctcatgatct ttgccctctc cattgttttc gtttttctgt ttctcgccgc tttgtatgaa
     2761 agctggcagg tccccttctc cgtattgctc gcagtgccta ttggcctgac cggagccata
     2821 ctcacgttga cgatcattcc ccatatggtc aataatgtgt atgcccagat cggaatgatt
     2881 accctgatcg ggttgtcggc gaaaaatgcg atccttattg tggaattcgc caaagagcgg
     2941 gtggataggg gattacctat cattcaatcg acgctggatg cagttaagct caggctaaga
     3001 ccgatcctga tgacttccct ggctttcatc ctgggcgtat tgccgctcgc gctggctacg
     3061 ggcgcagcaa gcgtagcgcg ttccaccata ggatggacag tattgggggg aatgcttgcc
     3121 gccacctcgc tggctatttt cattgttccg gttttgttcg tggtgatcac gcgtttttcc
     3181 tacagcaagc ggaaattgaa tgaattgaaa gaacatgtgg tttcggggct gaccgaatga
     3241 taaccgggtc cggctgaata aaataaaaga ggctgtctcc attaaatgag acagcctctt
     3301 ttattttatt tttttgcggt taccttttat ctcaggttaa tctggcgggt aatacttccc
     3361 accgccaatt cagcggaatg atcacaggtg ccatctccga aatcaatggt gccgttcagg
     3421 ctctgattga aaacaaaaga aatcacaccg gcactaacat aggggcatat gaacgctttg
     3481 gtcagcggcg tgctgacggt cgaaaccagg ctggtgccat ccgatgcgga aaaattactg
     3541 ttaccggtaa tgctgtaaac gtcgtccaat acattatagg gagtgccgct gccggcggta
     3601 agcgtgattg ttttattccc gctgtaatgg aaattggtca gatcaggaaa ggtgaatatc
     3661 ccgttgctaa cctgggacgt gaagctaaaa ccagccaggg tcgttgtatt ggtgatcgtg
     3721 caggatccct gaagtccgta accattcaca aaataatggt cgaaggttgc cgaaaccgat
     3781 gaaccgggga gaaccagggg accggtaaag acataaatga tctttccctg gcgcctgatg
     3841 ctgtcgatac tgttggaaca tccatccgct ccaaaatcca gggtaagcgt gatcggatat
     3901 cctgtaccct gggtggtatc cacggtatag gacgcacagc tcaaataaat atgattcatc
     3961 acgccaaccg cattagtggt tgttcctccc tgcctcaata cgccggatcc tccggtcaac
     4021 aggttgtctg aataaccata ataggcattt acgaatacat cgttataggc attgtcggcc
     4081 acggttgatg aatcggaaat actggctgcc gccgcatcgt cgctcttaag tgaattgccg
     4141 atatcctttt tgcaggaagt cgtaaaaaac agtgctgaaa tagcaaggat cttgccggac
     4201 aggtaaacgg aacggaatct catattttct ggatatttag ttaatctatt ctaacgtatc
     4261 cgatttccgc ttattttacc gggttcctaa aggaagggag atttattcct aatgcattga
     4321 taactaatag ataattcttc cgtcccgctt tggggagaaa aaaccggcct tagattcttt
     4381 ccaggtttga gaagaaaaaa tcacattcaa tccgtgcatt ttcatcactg tcgctgccat
     4441 gaatggcatt ttcacccagg gaggcggcaa aaagttggcg gatggtgccc gggtcggctt
     4501 ttgccggatc ggtcgccccg atgagcttcc gaaaatcagc aacggcattc tctttttcga
     4561 ggatggcggc gacgatgggt ccccggctca tgaagccgac cagttcgccg taaaaaggac
     4621 gggcctggtg aacggcatag aattctcctg cctttgcctc agaaagcgtg gtcattttca
     4681 acgcggtcag tttgaatcct gccttgataa taatgtccag gattgcgccc gaatggccgt
     4741 tcgcaaaggc atcgggcttg atcatcgtga acgtacggtt actcatttgt ttttgtttgg
     4801 cgggcaaagg taatacattt tgcagcagat ttgggaatgg tcatattatt ctgcaccttt
     4861 gccaaccatt ttatatgcag cagattacgg aaatatttcc cttactcagg acgcccaaat
     4921 ccatggtgat tgtaatgcac cagaaaccgg atgcggatgc catgggttca tccctcgggc
     4981 tttatcatat actcgtcgcc ctgggtcact cggttacggt tatttctccg accaactggg
     5041 ccgactggct caaatggatg aaagctgccg ataaggtgat cgactacgaa ctgaacacgg
     5101 caaaaggcaa tgaggcgttg gaaaaggcag aatggatttt ctgcctcgac ttcaataccc
     5161 tggcccgttc aagaaaaatg gctgaaccga tcgcccgctc ctccgctgtc cggat
//
LOCUS       DASYCU010000664         7936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Puia sp. isolate SMAG_U4941 k141_5071788, whole genome
            shotgun sequence.
ACCESSION   DASYCU010000664 DASYCU010000000
VERSION     DASYCU010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958215
            Sequence Read Archive: SRR11833507
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Puia sp. (soil metagenome)
  ORGANISM  Puia sp.
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Puia.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 12:33:16
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,686
            CDSs (total)                      :: 2,672
            Genes (coding)                    :: 2,671
            CDSs (with protein)               :: 2,671
            Genes (RNA)                       :: 14
            tRNAs                             :: 12
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Puia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5071788"
                     /isolate="SMAG_U4941"
                     /isolation_source="soil"
                     /db_xref="taxon:2045100"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="37 N 122.2 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..601
                     /locus_tag="VGM24_13390"
     CDS             134..601
                     /locus_tag="VGM24_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY4156427.1"
                     /translation="MKKIILALAVILIAGLSSGFANTPVDINKKAAAAFHADFRKAKD
                     VKWVESSKYIQVQFNLDDKIRYAYYSPDGRFICQTRNILTTDLPANLRYDVKKNYGSY
                     WVSELFELSTDQGVSYYVRLENGEGSVTLSADSGDSWRTYKMPESSAKRTVSL"
     gene            738..1136
                     /locus_tag="VGM24_13395"
     CDS             738..1136
                     /locus_tag="VGM24_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY4156428.1"
                     /translation="MDDQTVSVRYIVHDVDEAIAFYKDFLGFELLMHPAPPFAIIARG
                     NLRLLLSQPGSGGGGHAASDGQLPEPGGWNRIHIPVDDLETVFRDLKSKGAGFKSEMV
                     QGVGGKQVLLLDPSGNLVELFEYTMEKGKG"
     gene            1203..2594
                     /gene="rmuC"
                     /locus_tag="VGM24_13400"
     CDS             1203..2594
                     /gene="rmuC"
                     /locus_tag="VGM24_13400"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY4156429.1"
                     /translation="MSTTVILLCLVLALLIFGAGWLLAAFRWKSRSDREAEEKIRSQI
                     LYEKLQQDYSTLNRENSRLKDQEESLRLKEKETAHSLAGITAKYESALVAIQEQKEFV
                     GQAQQSLRDSFHALSASALDQNNKSFLDLAKTALTSHVSETKNDLDKRQQAIDNLVKP
                     LGTSLEKMEEKISQLELKREGAYGHLNGLLDQMRLTTQALDKETRNLVSALKTSHTRG
                     RYGEIALRRLVEFSGMLDQCDFLEQQSVDGENGRLRPDLIIRLPGSRSVVVDSKVPLS
                     AYLQIFETEDALLQKQLVAEHILAIKTHLRQLSAKAYWDQFDEAPDFVVLFMQIESSF
                     GAALQAWPGLIEEALNNRIIVATPTTLITILRSIGYSWNQLRTMENIETIREAAVELY
                     ERSATLMEHIAHIGKGLQGTLTHYNKAVGSLESQFLPQARRIQQLSQAYVKKNLPGME
                     PVETMVRELPEKQ"
     gene            2631..3863
                     /locus_tag="VGM24_13405"
     CDS             2631..3863
                     /locus_tag="VGM24_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY4156430.1"
                     /translation="MQKTLVVAGGGAAGFFCAVTAARLNPQWRVLIVEKTGKLLSKVK
                     VSGGGRCNVTHACFSIPEMIRKYPRGASFLKKTFHQFFTTDIIQWFEERGVKLKQEAD
                     GRMFPVSNSSQTIIDCLLEEAAKYKVEVRLHHEIRSIHQDESGSPFTLHFSNDWILEA
                     DFLCIASGGYPKASMFEWIKQMGHGIEEPVPSLFTFNMPGNSITKLMGVSVPDALVKV
                     NGTALKESGPLLITHWGLSGPAVLKLSAWGARLLKEKNYRFEISVNWLPQYHEQSLRE
                     EFQQARQESGARKITHKNPWDLPQRLWEWLLQQTGIGDIHWADLPAALQNKLIQNLCH
                     QAFAVEGKTTFKEEFVTAGGVRLAEINPQTMESRIRPGLFFAGEVMDVDGITGGFNFQ
                     HAWTSGFIAGKSVAGYRT"
     gene            3950..4483
                     /locus_tag="VGM24_13410"
     CDS             3950..4483
                     /locus_tag="VGM24_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY4156431.1"
                     /translation="MKPIIAILLLLAGTGSSNAQNANTMTLKEQMLKQLKTTHNQEEW
                     FAPLNAAIEGLTPAQANWKQNGADHSIAELTTHIIFWNSERLAKFMDQPPPAFSGNND
                     ETFSAVSENDWPVIVKRADSVLAAWEKAIEGADETKLAQWYETIGHISTHNAYHTGQI
                     VYIRKVQGSWNPAKGVK"
     gene            4494..4667
                     /locus_tag="VGM24_13415"
     CDS             4494..4667
                     /locus_tag="VGM24_13415"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HEY4156432.1"
                     /translation="MEEIEVPTEHLHETIEERVEETIAISAIAILTRKKILWFAGLCI
                     SCVGLFFFISGLF"
     gene            4790..5161
                     /locus_tag="VGM24_13420"
     CDS             4790..5161
                     /locus_tag="VGM24_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY4156433.1"
                     /translation="MRGIKTIVFLLISNTFMTFAWYGHLKFKDFSWGKSLGLFTVILI
                     SWGLAFFEYLFQVPANRAGFKEDGGPFTLVQLKTIQETITLTVFVGFTLLFFKEEKLA
                     WNHLVGFCFLIAAVFFIFKKW"
     gene            5271..7529
                     /locus_tag="VGM24_13425"
     CDS             5271..7529
                     /locus_tag="VGM24_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY4156434.1"
                     /translation="MRKIASILLLFLIFFSLSCKHKRSGNPAVLVYAQNLSSSGSGSW
                     VQAINKLGQKNGFDVRVTDTSLLFSDDSLQNFAAVVFLNCSGMPLNYMARIALERYIQ
                     AGGGFAGIHGPVKTFDWRWYGRMIAADSSVPAIFHDFDGGRAFYTSDTINEQSVDSPD
                     DLKRILNGIEYAIGDYQPLDYSKAKTQYPPSENHFTKHVLVQGTFFEPTEMTILPNLD
                     ILIIQRRGGILIYKHESGEVKQAGFLNVYWKTLHTKGVNAEEGVLGLSKDPSFSKNHW
                     VYIYYSPVDSSVNRLSRFTLLNDSIQNSSEKVILEVKAQREICCHTGGSIAFGPDSLL
                     YFSAGDNSTPFDEPGARYVSHGFAPLNDVPGHQQYDAERSAGNSNDLRGKIMRIRVHD
                     DGTYEIPKGNLFPPGTPKTRPEIYVMGDRNPYRISVDQKNSFLYWGEVGPDAGSDSLA
                     TRGPRGYDEINQARKAGFFGWPFFVGNNYPYRAYDYETGKSGPPFDPLKPENHSRNNT
                     GLVDLPPAQPAFIWYPYAHSPEFPDVGTGGRNAMAGPVYYTDMFPEATRYPDYYNGKL
                     FIYDWIRGWIKAVTLQPNGDFEQMDPFMEHTQLHNCIDMEVGPDGKLYLLEYGTGWFA
                     KNPDAGLSRIDYSAAVPTPAQPEAAATADSSAAAHGPFAAGYALTQVLDCKSCHKENA
                     VSIGPSFMKVAEKYAKDKKAEAYLSQKIMKGGSGVWGDVAMSAHPDIAKKDLDAIISY
                     ILSLNGTGKAKH"
     gene            7575..>7936
                     /locus_tag="VGM24_13430"
     CDS             7575..>7936
                     /locus_tag="VGM24_13430"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY4156435.1"
                     /translation="MKRLVCVLAGAILFLAGRSQVIGKISGIVTDENKVPVKWATIHI
                     WNTDISVISNAKGEFNIRPLPEGRYTIEISAVGFATVNREVNASGSESALAIRLKEAG
                     TRLDEVLVTAQKKEEDLQ"
BASE COUNT         2093 a         1937 c         2050 g         1856 t
ORIGIN      
        1 caaaatgaaa atcactgatc aggattccgt tccagaaaaa aaaataaaaa atatttcaac
       61 aagctgcaac atcattcaat aacccgcgtc ttattaagca gagttaaatt aaaacaacgt
      121 aaaaatctga aaaatgaaaa agatcattct ggcacttgca gtgatcctga tagccggcct
      181 cagcagcggc tttgccaata ctcctgttga tatcaataaa aaagccgcag ctgcatttca
      241 tgcggatttc agaaaggcaa aagatgtaaa atgggtggag tcttcaaaat acatacaggt
      301 tcagttcaac ctggacgata agatcaggta tgcctattat agtcccgacg gccgctttat
      361 ctgccagacg cgcaatatcc tgaccaccga tctgccggcc aatctgcgtt acgacgtcaa
      421 aaagaattat ggttcatact gggtttccga actctttgaa ctgagcacag accagggcgt
      481 ttcttactac gtcagactgg aaaatggaga aggctccgta acgctcagtg cggatagtgg
      541 agatagctgg agaacctata agatgccgga atcatccgcc aaacgaactg tatcactata
      601 gcaactttgt aattctgagt ttaggtttaa attcctccct ccgtttttac ggggggattt
      661 tttttactcc catgtcttcc ggatacaagg ccggcccctg attatttgta gctttattcc
      721 attaaacagc actatttatg gacgatcaaa cagtaagcgt tcggtacatt gttcatgatg
      781 tggatgaagc cattgctttt tacaaggatt ttctgggatt tgaattgctg atgcatcccg
      841 ctcctccgtt tgccatcatc gcccggggca atctccggct gctgctcagc cagcccggct
      901 ccggaggcgg aggccatgcc gcatccgacg gacaattgcc ggaacccggc ggttggaacc
      961 gcatccatat tccggtggac gatctggaaa ccgttttccg ggatctgaaa tcgaaaggag
     1021 ccggatttaa aagtgaaatg gtccagggag tcggcggcaa gcaggtcctt ctcctggatc
     1081 cttccggaaa cctggtcgaa ctttttgaat ataccatgga aaaaggcaag ggctaaagat
     1141 aaaagcggca aggggcggta tgctaatatt tttagtatct ttaccggtac aaaaattaac
     1201 ccatgagcac gactgtcatc ctgctatgcc tggtccttgc cctgctgatc tttggtgccg
     1261 gctggcttct ggccgctttc cgctggaaat ccaggtcgga ccgggaagcg gaagagaaga
     1321 tccgcagcca gatcttatat gaaaaactcc agcaggatta ttctacgctg aaccgggaga
     1381 acagccggtt aaaggaccag gaagaaagtt tacggttgaa agagaaagag accgctcact
     1441 cactggcagg gataaccgca aaatatgaat cggccctggt cgccattcag gaacaaaagg
     1501 agtttgtggg ccaggcccag caatccctca gagactcctt tcatgcatta tctgcttctg
     1561 cactggacca gaacaacaaa tcctttctcg acctggccaa aacagcgctc accagccatg
     1621 tttcggaaac aaaaaatgac ctggataaaa gacagcaggc catcgataac ctggtaaagc
     1681 cgctgggcac ttccctcgaa aaaatggagg agaagatcag tcagctcgaa ttaaaaaggg
     1741 aaggcgctta cggacatctc aacggcctgc tggaccagat gaggctgacc acgcaggcgc
     1801 tggacaagga aaccagaaac ctcgtcagtg ccttaaagac aagccatacg cgcggaagat
     1861 acggggagat tgccctgcgc cgcctggttg aattttccgg catgctggac cagtgcgatt
     1921 tcctggagca gcaatcggtg gacggagaga acgggagatt gcgccccgac ctgatcatcc
     1981 gcctgcccgg aagccggtcg gtggtggtgg acagcaaggt tccgctgagc gcctatctgc
     2041 agatatttga aacggaagat gcccttctgc aaaaacagtt agtggccgaa catattctgg
     2101 ccattaaaac ccatttgcgc cagctgagcg ccaaagccta ctgggatcag tttgacgaag
     2161 cacctgattt tgtggtcctg ttcatgcaga tcgaatcatc gttcggtgcg gcgctgcagg
     2221 cctggcccgg gctgatcgag gaagcgctga ataaccggat catcgtggcc accccgacca
     2281 cgctgatcac cattttaaga agcatcggct atagctggaa ccagctgcgc accatggaaa
     2341 atatcgaaac catacgcgag gcggcagtgg agctgtatga acgaagcgcc accctgatgg
     2401 aacatattgc ccatatcgga aagggattgc agggaaccct gacgcattat aataaagccg
     2461 tgggaagcct ggaaagccag ttccttcccc aggcccggcg gatacagcag cttagccagg
     2521 catatgtaaa gaagaatctg ccgggcatgg aaccggtgga aacgatggtc cgggagcttc
     2581 cggaaaaaca ataaaatttt tctcaccttc acggccgaca aacagaaagg atgcaaaaaa
     2641 cactggtggt tgccggaggc ggagcagccg gatttttctg tgccgttacg gccgcccgtc
     2701 tcaatccgca atggagggtg ctcatcgtgg agaagaccgg gaagttattg agcaaggtaa
     2761 aagtgagtgg cggcggacgc tgcaatgtaa cgcatgcctg tttttccatc ccggagatga
     2821 tccggaaata tccgagaggc gcttctttcc tgaagaaaac ctttcatcag ttctttacca
     2881 ccgatatcat tcaatggttt gaagaaaggg gagtgaagtt gaaacaggaa gccgacggga
     2941 gaatgttccc ggttagtaat tcctcccaaa ccattatcga ttgcctgctg gaggaagcgg
     3001 caaaatataa ggttgaggtc cggctgcatc atgagatccg gtccattcat caggatgaaa
     3061 gcgggtcgcc cttcaccctg catttttcca acgactggat cctggaggcc gactttctct
     3121 gcattgccag cggcggatat cccaaagcgt ccatgtttga atggataaaa caaatgggac
     3181 atggcatcga ggagccggtt ccctctttat ttacgtttaa tatgcccgga aattccatca
     3241 ctaaactaat gggggtttcc gttccggatg ccctggtaaa agtaaacggc accgccctga
     3301 aagaaagcgg ccctttattg atcacccatt ggggcctgag tggtcccgcc gttttaaaac
     3361 tctccgcctg gggagcccgg ttgctgaaag aaaagaatta ccggtttgag atctcggtaa
     3421 actggttgcc gcaataccat gaacaatccc ttcgggagga atttcagcag gcccgccagg
     3481 aaagcggcgc aagaaaaata acacacaaaa atccctggga ccttccccag agactctggg
     3541 aatggctcct tcagcagacc ggcatcgggg atattcattg ggccgacctt ccggcggccc
     3601 ttcagaataa gcttattcag aacctctgcc atcaggcttt tgccgtggaa ggaaaaacaa
     3661 cattcaaaga ggagtttgtg acagccggcg gcgttcgcct ggcggagatc aatccccaga
     3721 ccatggaaag ccggatcagg ccgggactat tttttgccgg agaggtcatg gacgtggacg
     3781 ggatcaccgg cggatttaat tttcagcatg cctggaccag cggtttcatt gcgggaaaat
     3841 ccgtcgccgg ataccggacc tgatccggcc ttattgcaaa attcttttcc ccggtaatcg
     3901 gcctttcact aacttatatc ttctttgttt ttcgcttaat tttctaacca tgaaacccat
     3961 cattgccata ctgctcctgc ttgcgggaac aggttcttcc aacgctcaaa atgcaaacac
     4021 catgacttta aaagagcaga tgctcaaaca actgaaaacg acccataacc aggaagaatg
     4081 gtttgcgccg ctgaatgcgg cgatcgaggg gttgacgccc gcgcaggcca attggaaaca
     4141 gaacggagcc gatcattcca tcgcggaact gaccactcat atcatcttct ggaattcaga
     4201 gcgcctggct aaatttatgg atcagccgcc ccctgctttt tcgggaaata acgatgaaac
     4261 cttctctgcg gtaagtgaaa acgactggcc ggttattgta aaacgggccg acagcgtgct
     4321 ggctgcctgg gaaaaagcga ttgaaggggc ggatgaaacc aaactggccc aatggtacga
     4381 aaccatcggg catatcagta cgcacaatgc gtatcatacc ggacagatcg tttatatccg
     4441 caaagtgcag ggttcctgga atccggccaa aggagtaaaa tagaaccgtt accatggaag
     4501 aaatagaagt gccgaccgaa catcttcacg aaaccattga agagcgggtg gaagagacca
     4561 ttgccatctc cgccatcgcc attctgaccc ggaaaaaaat actttggttt gccggccttt
     4621 gtatatcctg tgtcggatta ttctttttta tttccgggct gttctgatat cccgatcccc
     4681 gctgtatttt taattcaccc ggcaggtaaa tcatcctgcc ggattatggc gtaaatttga
     4741 agccgggacg ctttgtttcg ggtaaaacgc cgcagccata ttcatccata tgcgtggaat
     4801 caagaccatt gtatttctgc tgatatcaaa tacattcatg acgttcgcct ggtacgggca
     4861 tctcaagttc aaagattttt cctgggggaa gagcctgggt ttgtttaccg ttattctcat
     4921 cagctgggga ctggcttttt ttgaatacct gttccaggtc ccggccaacc gggcgggatt
     4981 caaagaggac ggtggtccct tcaccctggt tcagttgaaa acgattcagg aaacgatcac
     5041 ccttactgtt tttgtcggat tcacgctgct tttttttaag gaagaaaaac tggcctggaa
     5101 tcatctggtg gggttttgct tcctgatcgc cgctgttttt tttattttta agaaatggta
     5161 aacggcaaca gcgccgggat ttttttcgtc attaaccggc catttgtata aaaaatccgg
     5221 aaatccttgg ggttagtcct taagtttgca actatttaac ttttgccact atgaggaaaa
     5281 ttgccagcat cctgctcctg tttctgatct tcttttcatt atcctgcaaa cacaagcggt
     5341 ctggaaaccc ggccgtactg gtctacgctc agaacttaag ctcttccgga tccggatcct
     5401 gggttcaggc cataaacaaa ctggggcaga agaacgggtt tgatgtgcgg gttaccgata
     5461 cctcgctgct tttctcggat gactcccttc agaattttgc agccgtcgtg ttcttaaatt
     5521 gttccggcat gccgctcaat tacatggcgc ggatcgcact ggaaagatat atacaggccg
     5581 gcggcggttt tgccggtatt cacggacccg tgaaaacctt cgactggcgc tggtatggca
     5641 gaatgatcgc ggcggattcc agcgttccgg cgattttcca cgattttgac gggggaaggg
     5701 ctttctatac ttccgatacc atcaacgagc aaagcgttga cagtcccgac gatctgaaga
     5761 gaatactcaa cggtattgaa tatgcgatcg gggattatca gccgctggat tattccaaag
     5821 caaaaacgca atatcctcct tcggaaaatc attttacaaa acatgtcctg gtgcagggta
     5881 ctttttttga acccacggaa atgaccatcc ttcccaatct ggacatcctg attatccagc
     5941 ggcgcggagg gatcctcata tacaaacatg aatcgggtga ggtaaagcag gcgggatttc
     6001 tgaatgttta ctggaagacc cttcatacca aaggggtcaa tgcggaagaa ggcgtcctgg
     6061 gcctttccaa agatccctca ttcagcaaaa atcactgggt atatatttat tatagtccgg
     6121 tggacagttc ggtaaaccgg ttgtcccggt tcacgctcct caacgacagc atccagaatt
     6181 catcggagaa ggtgatcctg gaagttaaag cacaaagaga gatctgttgc catacggggg
     6241 gatcgattgc tttcggcccg gacagcttat tgtatttttc cgccggtgat aactctacgc
     6301 cgtttgatga acccggagca agatatgtca gtcatggttt tgccccgtta aatgacgtgc
     6361 ccggtcatca gcagtatgat gcggagcggt cggccggaaa ttcgaatgac ctgcggggaa
     6421 agatcatgcg tatccgggtt catgatgatg ggacctatga aattccgaaa gggaatctct
     6481 ttcccccggg aacgcccaaa accaggcccg agatttatgt gatgggcgac cgcaatccgt
     6541 accggatttc cgttgaccag aaaaatagtt tcttatactg gggagaagtt ggcccggatg
     6601 ccgggagtga cagcctggct acgcgcggtc cccggggata cgatgagata aaccaggcga
     6661 gaaaagccgg gttcttcgga tggccctttt ttgtcggtaa taattatcct taccgggcct
     6721 atgattatga aaccggcaag agcgggccgc ccttcgatcc gttgaaaccg gagaatcact
     6781 ccaggaacaa taccggtctc gttgacctgc ctccggcaca gccggccttc atctggtatc
     6841 cttacgctca ttcccccgaa ttcccggacg tgggcacggg tggacggaat gcgatggccg
     6901 gaccggtcta ttatacggat atgtttccgg aagccacccg ttatcccgat tattacaatg
     6961 gaaaattatt catatacgac tggatacgcg gatggatcaa ggccgtaacg cttcagccca
     7021 acggggattt cgaacagatg gatccgttca tggaacatac ccagctgcat aattgcattg
     7081 atatggaagt ggggcccgac ggaaaattat atttactgga atacggtacg ggctggtttg
     7141 caaagaatcc cgatgccggt ctttcccgga tagattatag cgcggccgta cccacgcctg
     7201 cccagccgga agccgcagcg actgccgatt cctctgccgc agcccatggg ccctttgcgg
     7261 cgggatatgc gcttacccag gttttggact gcaaaagctg ccataaggag aatgccgttt
     7321 ctatcggacc ttcctttatg aaggtggcgg aaaaatatgc aaaggacaag aaggcggagg
     7381 cctacctgag ccagaaaatt atgaaaggcg gaagcggggt gtggggcgat gtggcgatgt
     7441 cggcacatcc ggatatcgcc aaaaaggatt tggatgctat catcagttat attctctccc
     7501 tgaacggaac cgggaaagcc aaacactaag gcagcgatga caaagaaaag gagagaccaa
     7561 aatttgaaat ggaaatgaag agattagtgt gtgtcctggc aggagctata ctgtttttgg
     7621 cgggccggag tcaggtaatc gggaaaattt cagggatcgt tacggatgaa aacaaggtcc
     7681 cggttaaatg ggcgacgatc catatctgga atacggatat atccgtgatc agcaatgcaa
     7741 aaggggaatt caatatccgg ccgctgcccg aaggccggta taccatagaa atttccgccg
     7801 tcgggtttgc taccgtcaac cgggaagtga acgcttccgg atcggaaagc gcgctcgcca
     7861 ttcggcttaa ggaagccggg acccgactgg atgaagtgct ggtgaccgca caaaaaaagg
     7921 aagaggacct gcaaca
//
